研究者詳細

顔写真

ウヌマ タツヤ
鵜沼 辰哉
Tatsuya Unuma
所属
大学院農学研究科 生物生産科学専攻 水圏生産科学講座(水圏動物生理学分野)
職名
教授
学位
  • 博士(農学)(東北大学)

  • 修士(農学)(東北大学)

経歴 11

  • 2023年4月 ~ 継続中
    東北大学 大学院農学研究科

  • 2021年4月 ~ 2023年3月
    国立研究開発法人水産研究・教育機構水産資源研究所 社会・生態系システム部

  • 2020年7月 ~ 2021年3月
    国立研究開発法人水産研究・教育機構水産技術研究所 沿岸生態システム部

  • 2016年4月 ~ 2020年6月
    国立研究開発法人水産研究・教育機構北海道区水産研究所 生産環境部

  • 2011年4月 ~ 2016年3月
    独立行政法人水産総合研究センター北海道区水産研究所 生産環境部

  • 2009年4月 ~ 2011年3月
    独立行政法人水産総合研究センター北海道区水産研究所 海区水産業研究部

  • 2006年4月 ~ 2009年3月
    独立行政法人水産総合研究センター日本海区水産研究所 業務推進部

  • 2003年4月 ~ 2006年3月
    独立行政法人水産総合研究センター養殖研究所 生産技術部

  • 2002年4月 ~ 2003年3月
    独立行政法人水産総合研究センター養殖研究所 繁殖部

  • 1998年10月 ~ 2002年3月
    水産庁養殖研究所 繁殖部

  • 1992年4月 ~ 1998年9月
    水産庁養殖研究所 栄養代謝部

︎全件表示 ︎最初の5件までを表示

学歴 2

  • 東北大学 大学院農学研究科

    1990年4月 ~ 1992年3月

  • 東北大学 農学部 水産学科

    1986年4月 ~ 1990年3月

研究キーワード 4

  • 栄養生理

  • 繁殖生理

  • 魚類

  • 棘皮動物

研究分野 1

  • ライフサイエンス / 水圏生産科学 / 水圏動物生理学

論文 100

  1. Changes in ATP-related compounds and freshness indices in sea urchin gonads during cold storage

    Yuko Murata, Takashi Matsuda, Hiroshi Hoshikawa, Tatsuya Unuma

    Fisheries Science 91 (4) 833-844 2025年4月26日

    出版者・発行元: Springer Science and Business Media LLC

    DOI: 10.1007/s12562-025-01876-4  

    ISSN:0919-9268

    eISSN:1444-2906

  2. The Sea Cucumber-Infecting Parasite Apostichocystis gudetama gen. nov. sp. nov. Expands Marine-Host-Specific Clade of Apicomplexans. 国際誌

    Anthony M Bonacolta, Joana Krause-Massaguer, Tatsuya Unuma, Javier Del Campo

    The Journal of eukaryotic microbiology 72 (3) e70013 2025年

    DOI: 10.1111/jeu.70013  

    詳細を見る 詳細を閉じる

    Unknown ellipsoid bodies, later classified as apicomplexan cysts, are prevalent in the ovaries of Japanese sea cucumbers (Apostichopus japonicus), where they can lead to lower fecundity in infected individuals and adverse effects on wild populations as well as aquaculture efforts for this endangered species. Apicomplexans are widespread and essential to marine environments, where they can affect the health and fitness of host populations. We performed genomic sequencing of recovered cysts to gain more ecological and evolutionary information on this parasite. We recovered this apicomplexan's complete nuclear ribosomal RNA (rrn) operon, the entire mitochondrial genome, and a partial apicoplast (relic chloroplast) genome. The rrn operon phylogeny revealed this parasite as being closely related to coccidian-like parasites of marine fish (ichthyocolids) and cnidarians (corallicolids), while organelle phylogenomics hint at a closer relation to the protococcidian Eleutheroschizon. Using this new phylogenetic context and previous morphological descriptions, we describe this parasite as Apostichocystis gudetama gen. nov. sp. nov. Mining available microbiomes reveal the presence of Apostichocystis spp. beyond its host range, alluding to other potential hosts or cryptic, closely related lineages. Its phylogenetic placement has important implications concerning the evolution of parasitism within Apicomplexa and the divergence of a marine-host-specific clade of coccidian-like parasites.

  3. ベニザケ養殖における効果的な色揚げ方法の検討

    山本剛史, 泉田大介, 矢野豊, 松成宏之, 伴真俊, 黒川忠英, 鵜沼辰哉

    日本水産学会誌 90 (5) 2024年

    ISSN:0021-5392

  4. Distribution pattern and habitat use of the protandrous shrimp Pandalus latirostris in relation to environmental characteristics in Akkeshi waters on the pacific coast of eastern Hokkaido, Japan

    Sayaka Ito, Toru Nakagawa, Norio Shirafuji, Takashi Yokota, Daisuke Izumida, Toshihiro Onitsuka, Hiroshi Kuroda, Tatsuya Unuma, Tsuyoshi Watanabe

    Regional Studies in Marine Science 67 2023年12月20日

    DOI: 10.1016/j.rsma.2023.103198  

    eISSN:2352-4855

  5. Dietary protein requirement for somatic growth and gonad production in the sea urchin Strongylocentrotus intermedius at different life stages

    Satomi Takagi, Natsuki Hasegawa, Tsuyoshi Watanabe, Yuichi Sakai, Tatsuya Unuma

    AQUACULTURE 575 2023年10月

    DOI: 10.1016/j.aquaculture.2023.739748  

    ISSN:0044-8486

    eISSN:1873-5622

  6. 高齢ウニ(キタムラサキウニ)生殖巣の大きさと色調の給餌飼育による改善 査読有り

    干川裕, 菅原玲, 奥村裕弥, 中島幹二, 鵜沼辰哉

    北海道水産試験場研究報告 (104) 1-11 2023年9月

  7. Extension of immature stage of Hemicentrotus pulcherrimus ovary for reduction of the bitter compound (pulcherrimine) by water temperature manipulation

    Yuko Murata, Hiroshi Hoshikawa, Tomonori Kaneta, Satoshi Katayama, Tomonori Azumaya, Tatsuya Unuma

    Fisheries Science 2023年

    DOI: 10.1007/s12562-023-01708-3  

    ISSN:0919-9268

    eISSN:1444-2906

  8. Repeated reaching of the harmful algal bloom of Karenia spp. around the Pacific shoreline of Kushiro, eastern Hokkaido, Japan, during autumn 2021

    Natsuki Hasegawa, Tsuyoshi Watanabe, Tatsuya Unuma, Takashi Yokota, Daisuke Izumida, Toru Nakagawa, Tadahide Kurokawa, Satomi Takagi, Tomonori Azumaya, Yukiko Taniuchi, Hiroshi Kuroda, Saho Kitatsuji, Kazuo Abe

    FISHERIES SCIENCE 88 (6) 787-803 2022年11月

    DOI: 10.1007/s12562-022-01642-w  

    ISSN:0919-9268

    eISSN:1444-2906

  9. 光周期調節によるキタムラサキウニMesocentrotus nudusの成熟促進—Promoting gonadal maturation by photoperiod manipulation in the sea urchin Mesocentrotus nudus

    石井 正孝, 鵜沼 辰哉, 向阪 信一, 増田 篤稔, 村上 克介

    Eco-engineering 34 (3) 59-69 2022年7月

    出版者・発行元: 生態工学会

    ISSN:1347-0485

  10. Controlling factors of large-scale harmful algal blooms with Karenia selliformis after record-breaking marine heatwaves

    Satomi Takagi, Hiroshi Kuroda, Natsuki Hasegawa, Tsuyoshi Watanabe, Tatsuya Unuma, Yukiko Taniuchi, Takashi Yokota, Daisuke Izumida, Toru Nakagawa, Tadahide Kurokawa, Tomonori Azumaya

    FRONTIERS IN MARINE SCIENCE 9 2022年7月

    DOI: 10.3389/fmars.2022.939393  

    eISSN:2296-7745

  11. Accelerated photoperiod promotes gonadal maturation in the sea urchin Strongylocentrotus intermedius

    Masataka Ishii, Tatsuya Unuma, Akio Masadate, Hiroshi Hoshikawa, Kazuhiro Takahashi, Shinichi Kosaka, Atsunori Masuda, Katsusuke Murakami

    FISHERIES SCIENCE 88 (2) 299-310 2022年3月

    DOI: 10.1007/s12562-022-01583-4  

    ISSN:0919-9268

    eISSN:1444-2906

  12. Modest Dietary Protein Requirement for Sea Urchin Gonad Production Demonstrated by Feeding Trials with Consideration of Protein Leaching

    Satomi Takagi, Kazuhiro Takahashi, Tomonori Kaneta, Akira Sugawara, Masanao Narita, Shinji Kato, Masaki Akino, Hirofumi Takeda, Natsuki Hasegawa, Yuji Machiguchi, Tatsuya Unuma

    AQUACULTURE NUTRITION 2022 2022年3月

    DOI: 10.1155/2022/3140222  

    ISSN:1353-5773

    eISSN:1365-2095

  13. Correction to: Effects of rearing temperature manipulation on oocyte maturation progress in Japanese eel (Fisheries Science, (2021), 87, 5, (681-691), 10.1007/s12562-021-01531-8)

    Toshiomi Tanaka, Shinji Adachi, Kazuharu Nomura, Hideki Tanaka, Tatsuya Unuma

    Fisheries Science 87 (5) 693-695 2021年9月

    DOI: 10.1007/s12562-021-01537-2  

    ISSN:0919-9268

    eISSN:1444-2906

  14. Effects of rearing temperature manipulation on oocyte maturation progress in Japanese eel

    Toshiomi Tanaka, Shinji Adachi, Kazuharu Nomura, Hideki Tanaka, Tatsuya Unuma

    FISHERIES SCIENCE 87 (5) 681-691 2021年9月

    DOI: 10.1007/s12562-021-01531-8  

    ISSN:0919-9268

    eISSN:1444-2906

  15. Accumulation of a bitter amino acid, pulcherrimine, in sea urchin ovaries and its relevance to the annual reproductive cycle

    Yuko Murata, Ken Saito, Katashi Sugimoto, Masato Kiyomoto, Tatsuya Unuma

    FISHERIES SCIENCE 87 (3) 371-381 2021年5月

    DOI: 10.1007/s12562-021-01496-8  

    ISSN:0919-9268

    eISSN:1444-2906

  16. Coccidian Parasite in Sea Cucumber (Apostichopus japonicus) Ovaries

    Tatsuya Unuma, Noriko Tsuda, Yuichi Sakai, Takashi Kamaishi, Sayumi Sawaguchi, Naoki Itoh, Keisuke Yamano

    BIOLOGICAL BULLETIN 238 (1) 64-71 2020年2月

    DOI: 10.1086/707807  

    ISSN:0006-3185

    eISSN:1939-8697

  17. Gametogenesis in regular sea urchins: Structural, functional, and molecular/genomic biology

    Charles W. Walker, Michael P. Lesser, Tatsuya Unuma

    Developments in Aquaculture and Fisheries Science 43 29-50 2020年

    DOI: 10.1016/B978-0-12-819570-3.00003-2  

    ISSN:0167-9309

  18. Compositions of extractive components in the testes and ovaries of various sea urchins: comparisons among species, sexes, and maturational status

    Yuko Murata, Hirokazu Yoshimura, Tatsuya Unuma

    FISHERIES SCIENCE 86 (1) 203-213 2020年1月

    DOI: 10.1007/s12562-019-01388-y  

    ISSN:0919-9268

    eISSN:1444-2906

  19. Effects of taurine-enriched early live food (rotifer and Artemia nauplii) on the survival and growth of walleye pollock larvae

    Toru Nakagawa, Hiroyuki Matsunari, Takashi Yokota, Hiroshige Tanaka, Tetsuichiro Funamoto, Sayaka Ito, Takeshi Yamamoto, Tatsuya Unuma

    Aquaculture Science 67 (2) 157-170 2019年6月

  20. A microplate incubation method for assessing egg quality of the barfin flounder: effects of well size and rearing medium on larval viability

    Tatsuya Unuma, Takashi Ichikawa, Sayumi Sawaguchi, Natsuki Hasegawa

    AQUACULTURE INTERNATIONAL 27 (1) 195-208 2019年2月

    DOI: 10.1007/s10499-018-0316-5  

    ISSN:0967-6120

    eISSN:1573-143X

  21. Enhancing the Commercial Quality of Edible Sea Urchin Gonads - Technologies Emphasizing Nutritive Phagocytes

    Charles W. Walker, S. A. Böttger, Tatsuya Unuma, Stephen A. Watts, Larry G. Harris, Addison L. Lawrence, Stephen D. Eddy

    Echinoderm Aquaculture 263-286 2015年11月6日

    DOI: 10.1002/9781119005810.ch12  

  22. Sea Urchin Aquaculture in Japan

    Tatsuya Unuma, Yuichi Sakai, Yukio Agatsuma, Takaaki Kayaba

    Echinoderm Aquaculture 75-126 2015年11月6日

    DOI: 10.1002/9781119005810.ch5  

  23. Dietary shifts in the juvenile sea urchin Strongylocentrotus intermedius associated with the development of digestive enzymes

    Toshihiro Onitsuka, Kentaro Niwa, Tatsuya Unuma, Yuya Umezu

    MARINE BIOLOGY 162 (4) 869-880 2015年4月

    DOI: 10.1007/s00227-015-2630-z  

    ISSN:0025-3162

    eISSN:1432-1793

  24. Fatty acid composition in sea cucumber Apostichopus japonicus fed with microbially degraded dietary sources

    Natsuki Hasegawa, Sayumi Sawaguchi, Masaharu Tokuda, Tatsuya Unuma

    AQUACULTURE RESEARCH 45 (12) 2021-2031 2014年11月

    DOI: 10.1111/are.12149  

    ISSN:1355-557X

    eISSN:1365-2109

  25. VARIATION IN MANILA CLAM (RUDITAPES PHILIPPINARUM) FECUNDITY IN EASTERN HOKKAIDO, JAPAN

    Natsuki Hasegawa, Sayumi Sawaguchi, Tatsuya Unuma, Toshihiro Onitsuka, Masami Hamaguchi

    JOURNAL OF SHELLFISH RESEARCH 33 (3) 739-746 2014年11月

    DOI: 10.2983/035.033.0308  

    ISSN:0730-8000

    eISSN:1943-6319

  26. Effects of diet and temperature on post-settlement growth and survival of the short-spined sea urchin Strongylocentrotus intermedius

    Toshihiro Onitsuka, Tatsuya Unuma, Tomohiko Kawamura

    AQUACULTURE RESEARCH 45 (1) 1-15 2013年12月

    DOI: 10.1111/j.1365-2109.2012.03198.x  

    ISSN:1355-557X

    eISSN:1365-2109

  27. Post-ovulatory oocyte aging induces spontaneous occurrence of polyploids and mosaics in artificial fertilization of Japanese eel, Anguilla japonica

    Kazuharu Nomura, Yukako Takeda, Tatsuya Unuma, Kagayaki Morishima, Hideki Tanaka, Katsutoshi Arai, Hiromi Ohta

    AQUACULTURE 404 15-21 2013年8月

    DOI: 10.1016/j.aquaculture.2013.04.016  

    ISSN:0044-8486

  28. Sea urchin gametogenesis - Structural, functional and molecular/genomic biology

    Charles W. Walker, M. P. Lesser, Tatsuya Unuma

    Developments in Aquaculture and Fisheries Science 38 25-43 2013年

    DOI: 10.1016/B978-0-12-396491-5.00003-4  

    ISSN:0167-9309

  29. 珪藻土とゼオライトを添加したフロック状のマナマコ用配合飼料

    鵜沼 辰哉, 野口 浩介, 澤口 小有美, 長谷川 夏樹, 町口 裕二

    水産増殖 61 (4) 331-339 2013年

    出版者・発行元: 日本水産増殖学会

    DOI: 10.11233/aquaculturesci.61.331  

    ISSN:0371-4217

    eISSN:2185-0194

    詳細を見る 詳細を閉じる

    フロック状のマナマコ用配合飼料を開発した。海藻粉末と市販のナマコ用配合飼料を混合し,ここに約2.4倍量の無機成分(珪藻土とゼオライト)を加え,海藻粉末に含まれるアルギン酸を粘結剤として攪拌しながら塩化カルシウムでゲル化することにより,フロック状に粗い粒子の集合した飼料を調製した。この試験飼料を体長約7.8 mm の稚ナマコに29日間与えたところ,生残率,日間成長率とも海藻粉末区,市販ナマコ用配合飼料区よりも有意に高かった。また,体重約96 g の親ナマコに42日間与えたところ,日間成長率は市販ナマコ用配合飼料区よりも有意に高く,生殖巣指数も高い傾向を示し,組織学的観察から卵形成がより進んだと考えられた。これらの結果から,本飼料は稚ナマコ育成,採卵用親ナマコ養成の双方に有効であると考えられた。

  30. Optimum temperature of rearing water during artificial induction of ovulation in Japanese eel

    Tatsuya Unuma, Sayumi Sawaguchi, Natsuki Hasegawa, Noriko Tsuda, Toshiomi Tanaka, Kazuharu Nomura, Hideki Tanaka

    AQUACULTURE 358 216-223 2012年8月

    DOI: 10.1016/j.aquaculture.2012.07.004  

    ISSN:0044-8486

  31. Fusion of lipid droplets in Japanese eel oocytes: Stage classification and its use as a biomarker for induction of final oocyte maturation and ovulation

    Tatsuya Unuma, Natsuki Hasegawa, Sayumi Sawaguchi, Toshiomi Tanaka, Takahiro Matsubara, Kazuharu Nomura, Hideki Tanaka

    AQUACULTURE 322 142-148 2011年12月

    DOI: 10.1016/j.aquaculture.2011.10.001  

    ISSN:0044-8486

  32. Accumulation of the Major Yolk Protein and Zinc in the Agametogenic Sea Urchin Gonad

    Tatsuya Unuma, Sayumi Sawaguchi, Keisuke Yamano, Hiromi Ohta

    BIOLOGICAL BULLETIN 221 (2) 227-237 2011年10月

    DOI: 10.1086/BBLv221n2p227  

    ISSN:0006-3185

    eISSN:1939-8697

  33. 個別飼育法によるウナギの卵質評価で用いる添加物が形態異常に及ぼす影響

    鵜沼 辰哉, 黒川 忠英, 徳田 雅治, 野村 和晴, 田中 秀樹

    水産増殖 59 (2) 307-313 2011年

    出版者・発行元: 日本水産増殖学会

    DOI: 10.11233/aquaculturesci.59.307  

    ISSN:0371-4217

    eISSN:2185-0194

    詳細を見る 詳細を閉じる

    マイクロプレートを用いた個別飼育法はウナギの受精・ふ化・生残率測定に有効だが,ふ化直後の浮上斃死を防止する物質および抗生物質が欠かせないため,形態異常の解析に応用するにはこれらの物質が異常を誘発しないことが条件となる。48穴プレートにポリエチレングリコール6,000(PEG),ウシ血清アルブミン(BSA),ペニシリンGカリウム・硫酸ストレプトマイシン混合液(それぞれ200,000 IU/ml,0.2 g/ml)を加えた海水と受精卵を入れ,摂餌開始期まで飼育した。PEG と BSA は1μg/ml 以上で浮上斃死を止め,濃度上昇にともなう異常の増加はなかった。抗生物質混合液は0.25μl/ml 以上で細菌の増殖を止め,1μl/ml までは異常の増加はなかったが,2.5μl/ml 以上では体屈曲増加の傾向があった。以上の結果から,これらの物質を有効濃度内で低濃度で用いれば,本法により形態異常出現状況を評価できると判断された。

  34. A genetic linkage map of the Japanese eel (Anguilla japonica) based on AFLP and microsatellite markers

    Kazuharu Nomura, Akiyuki Ozaki, Kagayaki Morishima, Yukio Yoshikawa, Hideki Tanaka, Tatsuya Unuma, Hiromi Ohta, Katsutoshi Arai

    AQUACULTURE 310 (3-4) 329-342 2011年1月

    DOI: 10.1016/j.aquaculture.2010.11.006  

    ISSN:0044-8486

    eISSN:1873-5622

  35. The role of the major yolk protein in sea urchin reproduction and its relevance to aquaculture

    T. Unuma, C. W. Walker

    ECHINODERMS: DURHAM 437-444 2010年

  36. Molecular characterization of the major yolk protein of the Japanese common sea cucumber (Apostichopus japonicus) and its expression profile during ovarian development

    Atushi Fujiwara, Tatsuya Unuma, Kaoru Ohno, Keisuke Yamano

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 155 (1) 34-40 2010年1月

    DOI: 10.1016/j.cbpa.2009.09.002  

    ISSN:1095-6433

    eISSN:1531-4332

  37. The Sea Urchin Major Yolk Protein Is Synthesized Mainly in the Gut Inner Epithelium and the Gonadal Nutritive Phagocytes Before and During Gametogenesis

    Tatsuya Unuma, Akifumi Nakamura, Keisuke Yamano, Yukio Yokota

    MOLECULAR REPRODUCTION AND DEVELOPMENT 77 (1) 59-68 2010年1月

    DOI: 10.1002/mrd.21103  

    ISSN:1040-452X

    eISSN:1098-2795

  38. Vitamin contents of eggs that produce larvae showing a high survival rate in the Japanese eel Anguilla japonica

    Hirofumi Furuita, Tatsuya Unuma, Kazuharu Nomura, Hideki Tanaka, Tsuyoshi Sugita, Takeshi Yamamoto

    AQUACULTURE RESEARCH 40 (11) 1270-1278 2009年7月

    DOI: 10.1111/j.1365-2109.2009.02225.x  

    ISSN:1355-557X

  39. Vitamin content and quality of eggs produced by broodstock injected with vitamins C and E during artificial maturation in Japanese eel Anguilla japonica

    H. Furuita, T. Ishida, T. Suzuki, T. Unuma, T. Kurokawa, T. Sugita, T. Yamamoto

    AQUACULTURE 289 (3-4) 334-339 2009年4月

    DOI: 10.1016/j.aquaculture.2009.01.032  

    ISSN:0044-8486

  40. The Major Yolk Protein is Synthesized in the Digestive Tract and Secreted Into the Body Cavities in Sea Urchin Larvae

    Tatsuya Unuma, Kooichi Konishi, Masato Kiyomoto, Valeria Matranga, Keisuke Yamano, Hiromi Ohta, Yukio Yokota

    MOLECULAR REPRODUCTION AND DEVELOPMENT 76 (2) 142-150 2009年2月

    DOI: 10.1002/mrd.20939  

    ISSN:1040-452X

    eISSN:1098-2795

  41. Exogastrulation and interference with the expression of major yolk protein by estrogens administered to sea urchins. 国際誌

    Masato Kiyomoto, Ayumi Kikuchi, Seiko Morinaga, Tatuya Unuma, Yukio Yokota

    Cell biology and toxicology 24 (6) 611-20 2008年12月

    DOI: 10.1007/s10565-008-9073-y  

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    Although estrogens have been detected in some echinoderm species, their role is not clearly understood; so we examined the effects of estrogens administered to sea urchin embryos and larvae. A typical malformation was exogastrulation, induced by the exposure to ethynylestradiol (EER) in a defined period of 12 h from 12 h after fertilization (HAF). Morphogenesis for gastrulation was delayed in the treated embryos: protrusion of the archenteron started at 30 HAF when gastrulation had already finished in normal embryos. Exogastrulation induced by EER was cancelled by the antiestrogen chemical, ICI182,780. Feeding larvae were less sensitive to estrogens than those in early embryogenesis and, at certain concentrations, developed without abnormal morphology. The effect of estrogens was examined at the level of gene expression of the major yolk protein (MYP). MYP expression started during the larval stage and was suppressed by estrone at the six-armed stage, but not by beta-estradiol, and in later stage larvae, the expression was not affected by treatment with either estrogen. Estrogens affect sea urchins in the early stage of embryogenesis, leading to abnormal morphogenesis and interference with gene expression.

  42. Influence of Water Temperature on Morphological Deformities in Cultured Larvae of Japanese Eel, Anguilla japonica, at Completion of Yolk Resorption

    Tadahide Kurokawa, Takuma Okamoto, Koichiro Gen, Susumu Uji, Koji Murashita, Tatsuya Unuma, Kazuharu Nomura, Hajime Matsubara, Shin-Kwon Kim, Hiromi Ohta, Hideki Tanaka

    JOURNAL OF THE WORLD AQUACULTURE SOCIETY 39 (6) 726-735 2008年12月

    DOI: 10.1111/j.1749-7345.2008.00208.x  

    ISSN:0893-8849

  43. Occurrence of spontaneously spermiating eels in captivity

    Hajime Matsubara, Hideki Tanaka, Kazuharu Nomura, Tohru Kobayashi, Koji Murashita, Tadahide Kurokawa, Tatsuya Unuma, Shin-Kwon Kim, Mark P. Lokman, Takahiro Matsubara, Hirohiko Kagawa, Hiromi Ohta

    CYBIUM 32 (2) 174-175 2008年7月

    ISSN:0399-0974

  44. Zinc-binding property of the major yolk protein in the sea urchin - implications of its role as a zinc transporter for gametogenesis

    Tatsuya Unuma, Kazuo Ikeda, Keisuke Yamano, Akihiko Moriyama, Hiromi Ohta

    FEBS JOURNAL 274 (19) 4985-4998 2007年10月

    DOI: 10.1111/j.1742-4658.2007.06014.x  

    ISSN:1742-464X

  45. Chapter 2 Gametogenesis and reproduction of sea urchins

    Charles W Walker, Tatsuya Unuma, Michael P Lesser

    Developments in Aquaculture and Fisheries Science 37 11-33 2007年

    DOI: 10.1016/S0167-9309(07)80066-4  

    ISSN:0167-9309

  46. Lipid and fatty acid composition of eggs producing larvae with high survival rate in the Japanese eel

    H. Furuita, T. Unuma, K. Nomura, H. Tanaka, K. Okuzawa, T. Sugita, T. Yamamoto

    JOURNAL OF FISH BIOLOGY 69 (4) 1178-1189 2006年10月

    DOI: 10.1111/j.1095-8649.2006.01196.x  

    ISSN:0022-1112

  47. Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families

    K Nomura, K Morishima, H Tanaka, T Unuma, K Okuzawa, H Ohta, K Arai

    AQUACULTURE 257 (1-4) 53-67 2006年6月

    DOI: 10.1016/j.aquaculture.2006.03.011  

    ISSN:0044-8486

  48. Effects of ethynylestradiol and bisphenol A on the development of sea urchin embryos and juveniles

    M Kiyomoto, A Kikuchi, T Unuma, Y Yokota

    MARINE BIOLOGY 149 (1) 57-63 2006年4月

    DOI: 10.1007/s00227-005-0208-x  

    ISSN:0025-3162

  49. Expressed sequence tags from eyestalk of kuruma prawn, Marsupenaeus japonicus. 国際誌

    Keisuke Yamano, Tatsuya Unuma

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 143 (2) 155-61 2006年2月

    ISSN:1095-6433

    詳細を見る 詳細を閉じる

    We analyzed the expressed sequence tags (ESTs) obtained from a cDNA library of the eyestalk of the kuruma prawn, Marsupenaeus japonicus, to examine gene expression profile with special focus on female reproduction. The assembly of 1988 ESTs created 136 contigs from 738 ESTs; however 1250 ESTs remained singletons. Significant similarities (blast score > or = 50 bits) to the DNA sequences in the databank were found for only 16.7% of the 1386 sequences (136 contigs plus 1250 singletons), suggesting that the eyestalk library contains many unknown genes. Ribosomal RNA and mitochondrial respiration enzymes with significant similarities were found abundantly in the ESTs, whereas genes related to maturation or endocrine systems were scarce. Three ESTs were assumed to encode novel eyestalk hormones with marked similarities to pigment-dispersing hormone, molt-inhibiting hormone and crustacean hyperglycemic hormone. Sequences encoding a product highly homologous to farnesoic acid O-methyltransferase, an enzyme that produces methyl farnesoate, were also found.

  50. Microsatellite genotypes in triploid Japanese eel (Anguilla japonica) induced by heat shock 査読有り

    K Nomura, H Kagawa, H Tanaka, K Okuzawa, T Unuma, K Arai

    AQUACULTURE 247 (1-4) 20-20 2005年6月

    ISSN:0044-8486

  51. Relationship between egg specific gravity and egg quality in the Japanese eel, Anguilla japonica

    T Unuma, S Kondo, H Tanaka, H Kagawa, K Nomura, H Ohta

    AQUACULTURE 246 (1-4) 493-500 2005年5月

    DOI: 10.1016/j.aquaculture.2005.01.011  

    ISSN:0044-8486

    eISSN:1873-5622

  52. The first success of glass eel production in the world: basic biology on fish reproduction advances new applied technology in aquaculture

    Hirohiko Kagawa, Hideki Tanaka, Hiromi Ohta, Tatsuya Unuma, Kazuharu Nomura

    FISH PHYSIOLOGY AND BIOCHEMISTRY 31 (2-3) 193-199 2005年4月

    DOI: 10.1007/s10695-006-0024-3  

    ISSN:0920-1742

    eISSN:1573-5168

  53. Cathepsin C transcripts are differentially expressed in the final stages of oocyte maturation in kuruma prawn Marsupenaeus japonicus. 国際誌

    Gao-Feng Qiu, Keisuke Yamano, Tatsuya Unuma

    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 140 (2) 171-81 2005年2月

    ISSN:1096-4959

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    To elucidate the molecular mechanism of oocyte maturation in the kuruma prawn (Marsupenaeus japonicus), subtractive suppression hybridization (SSH) was initially used to identify novel up-regulated genes during the final stages of oocyte maturation, followed by evaluation of the differential expression profile by macroarray and quantitative real-time RT-PCR analyses. The cathepsin C (dipeptidyl peptidase I) gene was thus found to exhibit a significantly higher expression around the onset of cortical rod (CR) formation (early CR stage, appearance of round CRs), progress to a higher mRNA level until the middle CR stage (elongation of CRs), then rapidly revert to a low expression level at the late CR stage (occurrence of germinal vesicle breakdown, GVBD), as also observed at the non-CR stage (previtellogenesis and vitellogenesis). In situ hybridization analyses revealed that the sites of the expression of cathepsin C transcripts in the ovary were distributed in both oocyte and follicle cells, particularly at the early CR stage. A full-length cDNA sequence of this stage-specific gene was subsequently determined by rapid amplification of the cDNA 3' and 5' ends (3' and 5' RACE). The deduced amino acid sequence of the 230-residue mature peptide shared 67-70% identity to the known cathepsin C in mammals. Western blot analysis showed that expression of procathepsin C protein was exclusively at CR stages. The storage site of procathepsin C protein was localized in CRs as revealed by immunohistochemical analysis. This is the first report on the full-length cDNA sequence of cathepsin C and a demonstration of its involvement in the final stages of oocyte maturation in crustacean species.

  54. Determination of the rates of fertilization, hatching and larval survival in the Japanese eel, Anguilla japonica, using tissue culture microplates

    T Unuma, S Kondo, H Tanaka, H Kagawa, K Nomura, H Ohta

    AQUACULTURE 241 (1-4) 345-356 2004年11月

    DOI: 10.1016/j.aquaculture.2004.08.005  

    ISSN:0044-8486

  55. Molecular cloning and ovarian expression profiles of thrombospondin, a major component of cortical rods in mature oocytes of penaeid shrimp, Marsupenaeus japonicus. 国際誌

    Keisuke Yamano, Gao-Feng Qiu, Tatsuya Unuma

    Biology of reproduction 70 (6) 1670-8 2004年6月

    ISSN:0006-3363

    詳細を見る 詳細を閉じる

    In penaeid shrimp, cortical rods (CRs) are formed in peripheral crypts of the oocyte after completion of yolk accumulation; subsequently the CRs are utilized as a source of jelly materials that surround fertilized eggs. In our previous study, of five major components, three CR proteins displayed quite similar immunological characteristics. In this study, cDNA sequences and developmental expression profiles at both transcriptional and protein levels were examined to elucidate the molecular characteristics of CR proteins and the process of CR formation. Sequencing cDNAs exhibited the presence of three related forms that have identical sequences except for the loss of 246 and 369 bp in medium and short forms, respectively, suggesting that a single gene generates three transcriptional variants corresponding to the three CR proteins. Their deduced amino acid sequences revealed similarities to those of extracellular matrix proteins in a thrombospondin (TSP) 3,4/cartilage oligomeric protein family, and thereby the CR proteins were designated mjTSP. Semiquantitative analysis by real-time polymerase chain reaction revealed the presence of mjTSP transcripts, at similar levels, in immature, vitellogenic, and mature ovaries. Furthermore, in situ hybridization localized the majority of transcripts in previtellogenic oocytes in ovaries at all developmental stages. By the Western blot, on the other hand, mjTSP proteins were undetectable in immature ovaries but became obvious at the early vitellogenic stage. The immunosignals were enhanced during vitellogenic stages and maintained a high intensity in mature ovaries. Thus, transcription, translation of mjTSP, and formation of the CR structure occurred at different stages of ovarian development.

  56. Development of endocrine system regulating exocrine pancreas and estimation of feeding and digestive ability in Japanese eel larvae

    T Kurokawa, N Iinuma, T Unuma, H Tanaka, H Kagawa, H Ohta, T Suzuki

    AQUACULTURE 234 (1-4) 513-525 2004年5月

    DOI: 10.1016/j.aquaculture.2003.12.002  

    ISSN:0044-8486

  57. Induction of triploidy by heat shock in the Japanese eel Anguilla japonica

    K Nomura, J Nakajima, H Ohta, H Kagawa, H Tanaka, T Unuma, K Yamauchi, K Arai

    FISHERIES SCIENCE 70 (2) 247-255 2004年4月

    DOI: 10.1111/j.1444-2906.2003.00798.x  

    ISSN:0919-9268

  58. Occurrence of the sea urchin with pulcherrimine, a novel bitter amino acid

    Y Murata, M Yokoyama, T Unuma, NU Sata, R Kuwahara, M Kaneniwa, Oohara, I

    MORE EFFICIENT UTILIZATION OF FISH AND FISHERIES PRODUCTS 42 289-300 2004年

    ISSN:0167-4501

  59. Care and maintenance of adult echinoderms 国際誌

    S Anne Böttger, Charles W Walker, Tatsuya Unuma

    Methods in cell biology 74 17-38 2004年

    ISSN:0091-679X

  60. Immunological characterization of cortical rod proteins of kuruma prawn, Marsupenaeus japonicus. 国際誌

    Keisuke Yamano, Hisatake Seto, Gao-Feng Qiu, Tatsuya Unuma

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 136 (2) 371-377 2003年10月

    ISSN:1095-6433

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    The penaeid shrimp develops cortical rods (CRs) in the oocyte after completing yolk accumulation during ovarian maturation. In this study, CRs from the mature ovary of the kuruma prawn (Marsupenaeus japonicus) were isolated and their proteins were characterized. Under reducing conditions of SDS-PAGE, the CR demonstrated five major bands of approximately 210, 150, 140, 130 and 30 kDa. Of 32 clones of monoclonal antibodies raised against the CR proteins, 21 were found to be immunoreactive by Western blotting, demonstrating 150, 140 and 130 kDa proteins simultaneously. This result suggested that the proteins were post-translational or post-transcriptional products from the same gene, or that they originated from the same gene family. CRs were identified by immunohistochemical analysis by 31 clones of monoclonal antibodies in paraffin sections and by 26 clones in cryostatic ones. Most of the antibodies also stained the yolk in both developing and mature oocytes, suggesting that CR proteins accumulated in developing oocytes, then assembled to form the CR structure.

  61. Cleavage site of a major yolk protein (MYP) determined by cDNA isolation and amino acid sequencing in sea urchin, Hemicentrotus pulcherrimus. 国際誌

    Yukio Yokota, Tatsuya Unuma, Akihiko Moriyama, Keisuke Yamano

    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 135 (1) 71-81 2003年5月

    ISSN:1096-4959

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    The overall sequence of cDNA encoding vitellogenin (Vg), a precursor to major yolk protein (MYP), of Hemicentrotus pulcherrimus was determined. Its nucleotide sequence has an open reading frame of 4041 bp encoding 1346 amino acids. The amino acid sequence showed little similarity to other Vgs in vertebrates, insects or nematodes, but resembled members of the vertebrate and invertebrate transferrin family. The N-terminal amino acid sequence of the protein fragments dominant in the later embryonic stage was analyzed in order to determine the cleavage site of MYP. Determination of the cleavage site in MYP and analysis of MYP proteolysis in vitro suggested that MYP has a specific molecular shape to permit its proteolytic fragmentation at a definite site. The functional region of transferrin in MYP is conserved after proteolytic processing. Considering these results and those from other work, the protein called sea urchin Vg is not a true Vg. Therefore, a new name, echinoferrin, is proposed for this protein.

  62. Role of prostaglandin in the control of ovulation in the Japanese eel Anguilla japonica

    H Kagawa, H Tanaka, T Unuma, H Ohta, K Gen, K Okuzawa

    FISHERIES SCIENCE 69 (2) 234-241 2003年4月

    DOI: 10.1046/j.1444-2906.2003.00613.x  

    ISSN:0919-9268

  63. Changes in fertility of rainbow trout eggs retained in coelom

    T Azuma, H Ohta, S Oda, K Muto, T Yada, T Unuma

    FISHERIES SCIENCE 69 (1) 131-136 2003年2月

    DOI: 10.1046/j.1444-2906.2003.00597.x  

    ISSN:0919-9268

  64. Induction of sperm maturation

    H Ohta, T Unuma

    EEL BIOLOGY 415-423 2003年

    DOI: 10.1007/978-4-431-65907-5_28  

  65. Occurrence of spontaneous polyploids from the eggs obtained by artificial induction of maturation in the Japanese eel (Anguilla japonica)

    H Ohta, Y Higashimoto, S Koga, T Unuma, K Nomura, H Tanaka, H Kagawa, K Arai

    FISH PHYSIOLOGY AND BIOCHEMISTRY 28 (1-4) 517-518 2003年

    DOI: 10.1023/B:FISH.0000030648.83531.0c  

    ISSN:0920-1742

  66. The first production of glass eel in captivity: fish reproductive physiology facilitates great progress in aquaculture

    H Tanaka, H Kagawa, H Ohta, T Unuma, K Nomura

    FISH PHYSIOLOGY AND BIOCHEMISTRY 28 (1-4) 493-497 2003年

    DOI: 10.1023/B:FISH.0000030638.56031.ed  

    ISSN:0920-1742

  67. Biochemical composition of eggs in relation to egg quality in the Japanese eel, Anguilla japonica

    H Furuita, H Ohta, T Unuma, H Tanaka, H Kagawa, N Suzuki, T Yamamoto

    FISH PHYSIOLOGY AND BIOCHEMISTRY 29 (1) 37-46 2003年

    DOI: 10.1023/B:FISH.0000035897.58924.9d  

    ISSN:0920-1742

    eISSN:1573-5168

  68. Quantitative changes in yolk protein and other components in the ovary and testis of the sea urchin Pseudocentrotus depressus. 国際誌

    T Unuma, T Yamamoto, T Akiyama, M Shiraishi, H Ohta

    Journal of Experimental Biology 206 (2) 365-372 2003年1月

    ISSN:0022-0949

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    Both male and female sea urchins accumulate the major yolk protein (MYP; the most abundant yolk granule protein in sea urchin eggs) in the nutritive phagocytes of immature gonads before gametogenesis. In this study, quantitative changes in MYP as well as in other biochemical components in the ovary and testis were examined in the course of gametogenesis in Pseudocentrotus depressus. Before gametogenesis, both the ovary and testis contained large quantities of proteins, lipids and polysaccharides. MYP reached about 80% of total protein in both sexes. In the testis, MYP decreased rapidly as spermatogenesis proceeded, and the fully mature testis contained little MYP; the levels of lipids and polysaccharides also decreased. In contrast, the levels of nucleic acids and proteins other than MYP increased markedly. In the ovary, MYP decreased gradually as oogenesis proceeded, and the fully mature ovary contained less than half of the initial amount of MYP. Polysaccharides also decreased, whereas proteins other than MYP increased. These results, taken together with those from other studies, suggest that MYP serves as a protein reserve that accumulates before gametogenesis and is used as material for synthesizing new substances constituting gametes in both male and female sea urchins.

  69. Expression of pancreatic enzyme genes during the early larval stage of Japanese eel Anguilla japonica

    T Kurokawa, T Suzuki, H Ohta, H Kagawa, H Tanaka, T Unuma

    FISHERIES SCIENCE 68 (4) 736-744 2002年8月

    DOI: 10.1046/j.1444-2906.2002.00487.x  

    ISSN:0919-9268

  70. Seasonal changes of bitterness and pulcherrimine content in gonads of green sea urchin Hemicentrotus pulcherrimus at Iwaki in Fukushima Prefecture

    Y Murata, M Yokoyama, T Unuma, NU Sata, R Kuwahara, M Kaneniwa

    FISHERIES SCIENCE 68 (1) 184-189 2002年2月

    DOI: 10.1046/j.1444-2906.2002.00406.x  

    ISSN:0919-9268

  71. Gonadal growth and its relationship to aquaculture in sea urchins

    Tatsuya Unuma

    The Sea Urchin : from Basic Biology to Aquaculture 115-127 2002年

  72. Control by the environmental concentration of ions of the potential for motility in Japanese eel spermatozoa

    H Ohta, H Kagawa, H Tanaka, T Unuma

    AQUACULTURE 198 (3-4) 339-351 2001年7月

    DOI: 10.1016/S0044-8486(00)00597-4  

    ISSN:0044-8486

  73. Effects of bicarbonate ions and pH on acquisition and maintenance of potential for motility in ayu, Plecoglossus altivelis Temminck et Schlegel (Osmeridae), spermatozoa

    H Ohta, T Unuma, M Tsuji, M Yoshioka, M Kashiwagi

    AQUACULTURE RESEARCH 32 (5) 385-392 2001年5月

    DOI: 10.1046/j.1365-2109.2001.00571.x  

    ISSN:1355-557X

  74. Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depresses

    T Unuma, H Okamoto, K Konishi, H Ohta, K Mori

    ZOOLOGICAL SCIENCE 18 (4) 559-565 2001年5月

    DOI: 10.2108/zsj.18.559  

    ISSN:0289-0003

  75. Optimum protein level in a purified diet for young red sea urchin Pseudocentrotus depressus

    T Akiyama, T Unuma, T Yamamoto

    FISHERIES SCIENCE 67 (2) 361-363 2001年4月

    DOI: 10.1046/j.1444-2906.2001.00252.x  

    ISSN:0919-9268

  76. Cryopreservation of the sperm of the Japanese bitterling

    H Ohta, K Kawamura, T Unuma, Y Takegoshi

    JOURNAL OF FISH BIOLOGY 58 (3) 670-681 2001年3月

    DOI: 10.1006/jfbi.2000.1477  

    ISSN:0022-1112

  77. Reproduction of sea urchins

    C. W. Walker, T. Unuma, N. A. McGinn, L. M. Harrington, M. P. Lesser

    Developments in Aquaculture and Fisheries Science 32 (C) 5-26 2001年

    DOI: 10.1016/S0167-9309(01)80003-X  

    ISSN:0167-9309

  78. アカウニ餌料としてのアラメ栄養価の季節,海域および葉体部位による変化

    秋山 敏男, 山本 剛史, 鵜沼 辰哉, 篠原 直哉

    水産増殖 49 (4) 475-482 2001年

    出版者・発行元: 日本水産増殖学会

    DOI: 10.11233/aquaculturesci1953.49.475  

    ISSN:0371-4217

    eISSN:2185-0194

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    アカウニの主要な餌料海藻の一つであるアラメについて,粗タンパク量やエネルギー量の周年変化,アミノ酸組成の地理的・季節的差違,葉状体の部位による成分の違いを調査し,主としてタンパク質栄養の観点から評価を行った。<BR>三重県阿児町で採取されたアラメ葉体部の水分量,タンパク質量,およびエネルギー量は周年変動した。7-10月に乾物重量,粗タンパク量,およびエネルギー量はそれぞれ29-32%,3-4%,および360-370kcal/100g乾物試料と最も高くなった。一方,2月にはそれぞれ14%,2%,および270kcalと最も低い値が観察された。また,年間を通してアラメが稚ウニの餌料としてそのタンパク要求量(餌料中の20%)を満足した月は全くなかった。<BR>アラメ葉体中のアミノ酸の含有量やバランスには,海域(福岡県・三重県問)よりも季節の違い(三重県の5月と10月)の方が大きく影響した。また,葉体の部位によっても違いが認められ,葉片の新生部位の方が古い部位よりも必須アミノ酸バランスが良かったが,タンパク質量は低かった。<BR>必須アミノ酸バランスの点では,アラメのウニに対する栄養価はワカメやカゼインよりも劣っており,その最大の原因はアルギニン含量の低さにあった。従って,アカウニ養成の主要餌料としてアラメを使用する際にはアルギニン含量の高い配合飼料の併用が成長の促進に有効であるかもしれない。

  79. Voluntary intake of diets with varying digestible energy contents and energy sources, by juvenile rainbow trout Oncorhynchus mykiss, using self-feeders

    T Yamamoto, T Shima, T Unuma, M Shiraishi, T Akiyama, M Tabata

    FISHERIES SCIENCE 66 (3) 528-534 2000年6月

    DOI: 10.1046/j.1444-2906.2000.00083.x  

    ISSN:0919-9268

    eISSN:1444-2906

  80. The influence of dietary protein sources on tissue free amino acid levels of fingerling rainbow trout

    T Yamamoto, T Unuma, T Akiyama

    FISHERIES SCIENCE 66 (2) 310-320 2000年4月

    DOI: 10.1046/j.1444-2906.2000.00049.x  

    ISSN:0919-9268

  81. The influence of dietary protein and fat levels on tissue free amino acid levels of fingerling rainbow trout (Oncorhynchus mykiss)

    T Yamamoto, T Unuma, T Akiyama

    AQUACULTURE 182 (3-4) 353-372 2000年2月

    DOI: 10.1016/S0044-8486(99)00277-X  

    ISSN:0044-8486

  82. アマゴ精子の冷蔵保存用希釈液と媒精溶液の検討

    太田 博巳, 鵜沼 辰哉, 名古屋 博之

    日本水産学会誌 66 (1) 88-96 2000年

    出版者・発行元: 公益社団法人 日本水産学会

    DOI: 10.2331/suisan.66.88   10.1007/s12562-015-0899-y_references_DOI_GgopNbe2jc83C6vMS9U039e5uks  

    ISSN:0021-5392

    eISSN:1349-998X

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    精子の冷蔵保存用希釈溶液と保存精子の運動開始を導くための溶液の組成について検討した。保存用希釈液のK濃度は30mMが最も高い運動能を維持し, これは希釈時の運動開始を抑制する濃度と一致していた。また, 希釈液にはKに加えてCaの添加とpHの調整が不可欠であった。保存した精子の運動開始率をDW(pH8.0)と120mM NaHCO3液で比較したところ, ほぼ全ての保存条件でNaHCO3液の方が高い値を示した。この運動率の高さは同濃度のNaCl液, 人工体腔液でも認められ, 3者に有意な差は認められなかった。NaHCO3液の濃度を変えて比較したところ, 運動開始率の向上には30mM以上が, 運動の持続には60mM以上の濃度が有効であった。

  83. Effect of Steroids on Gonadal Growth and Gametogenesis in the Juvenile Red Sea Urchin Pseudocentrotus depressus. 国際誌

    T Unuma, T Yamamoto, T Akiyama

    The Biological bulletin 196 (2) 199-204 1999年4月

    DOI: 10.2307/1542565  

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    Red sea urchins, 10 months old, were fed for 30 days on a casein-based diet containing progesterone, androstenedione, testosterone, estrone, or estradiol-17{beta}. The mean gonad indices of male animals in the androstenedione-and the estrone-treated groups were significantly higher than those in the control group, suggesting that these steroids promote gonadal growth in male animals. Histological observations indicated that spermatogenesis in the estrone-treated group was also promoted compared to that in the control group. In contrast, female urchins were not obviously affected by the steroid-treated diets, probably because yearling female P. depressus are not otherwise ready to carry out gametogenesis. We conclude that androstenedione, estrone, and possibly their derivatives, are involved in the reproduction of male P. depressus.

  84. Postprandial changes in plasma free amino acid concentrations of rainbow trout fed diets containing different protein sources

    T Yamamoto, T Unuma, T Akiyama

    FISHERIES SCIENCE 64 (3) 474-481 1998年6月

    DOI: 10.2331/fishsci.64.474  

    ISSN:0919-9268

  85. Apparent and true availabilities of amino acids from several protein sources for fingerling rainbow trout, common carp, and red sea bream

    T Yamamoto, A Akimoto, S Kishi, T Unuma, T Akiyama

    FISHERIES SCIENCE 64 (3) 448-458 1998年6月

    DOI: 10.2331/fishsci.64.448  

    ISSN:0919-9268

    eISSN:1444-2906

  86. Apparent availabilities of amino acids from several protein sources for fingerling Japanese flounder

    Takeshi Yamamoto, Tatsuya Unuma, Toshio Akiyama

    Bulletin of National Research Institute of Aquaculture (27) 27-35 1998年3月

  87. A Protein Identical to the Yolk Protein Is Stored in the Testis in Male Red Sea Urchin, Pseudocentrotus depressus. 国際誌

    T Unuma, T Suzuki, T Kurokawa, T Yamamoto, T Akiyama

    The Biological bulletin 194 (1) 92-97 1998年2月

    DOI: 10.2307/1542517  

    詳細を見る 詳細を閉じる

    Female sea urchins store the major yolk protein (MYP) in ovarian nutritive phagocytes before vitellogenesis. Using immunological procedures, we detected MYP in the testicular nutritive phagocytes of Pseudocentrotus depressus, the red sea urchin, and then compared the distribution of MYP between sexes during gametogenesis. MYP was purified from unfertilized eggs by ion exchange chromatography (Q Sepharose) and gel filtration (Superdex 200), and an antiserum (anti-MYP) was raised against MYP. Immunoblot analysis demonstrated that immature testes, as well as ovaries, contained a large quantity of MYP. Immunohistochemistry showed that MYP was distributed in the nutritive phagocytes occupying the follicular lumen in both males and females. In both sexes, as gametogenesis proceeded, the nutritive phagocytes degenerated and the gonadal lumen filled with gametes. MYP accumulated in ripe ova as a yolk protein in the mature ovary. In contrast, MYP was not detected in mature testes, because stored spermatozoa did not react with anti-MYP. We conclude that in male P. depressus, MYP is stored in the testicular nutritive phagocytes and utilized as the nutrient source for spermatogenesis.

  88. Apparent availabilities of amino acids and minerals from several protein sources for fingerling rainbow trout

    T Yamamoto, K Ikeda, T Unuma, T Akiyama

    FISHERIES SCIENCE 63 (6) 995-1001 1997年12月

    DOI: 10.2331/fishsci.63.995  

    ISSN:0919-9268

    eISSN:1444-2906

  89. An evaluation of amino acid sources and binders in semipurified diet for red sea urchin Pseudocentrotus depressus

    T Akiyama, T Unuma, T Yamamoto, H Furuita, K Konishi

    FISHERIES SCIENCE 63 (6) 881-886 1997年12月

    DOI: 10.2331/fishsci.63.881  

    ISSN:0919-9268

  90. Effect of dietary tryptophan on maturation of ayu Plecoglossus altivelis

    T Akiyama, M Shiraishi, T Yamamoto, T Unuma

    FISHERIES SCIENCE 62 (5) 776-782 1996年10月

    DOI: 10.2331/fishsci.62.776  

    ISSN:0919-9268

  91. Utilization of malt protein flour in fingerling carp diets

    T Yamamoto, T Unuma, T Akiyama, S Kishi

    FISHERIES SCIENCE 62 (5) 783-789 1996年10月

    DOI: 10.2331/fishsci.62.783  

    ISSN:0919-9268

  92. 飼料へのクロレラエキス添加が早熟系アマゴの成熟に及ぼす影響

    鵜沼, 辰哉, 秋山, 敏男, 山本, 剛史, 新間, 脩子

    養殖研究所研究報告 = Bulletin of National Research Institute of Aquaculture (25) 55-58 1996年3月

    出版者・発行元: 水産庁養殖研究所

    ISSN:0389-5858

  93. Utilization of malt protein flour in the diets for fingerling red sea bream

    T Yamamoto, T Unuma, T Akiyama, S Kishi

    FISHERIES SCIENCE 62 (1) 59-63 1996年2月

    DOI: 10.2331/fishsci.62.59  

    ISSN:0919-9268

  94. Seasonal Changes in Gonads of Cultured and Wild Red Sea Urchin, Pseudocentrotus depressus

    Tatsuya UNUMA, Kooichi KONISHI, Hirofumi FURUITA, Takeshi YAMAMOTO, Toshio AKIYAMA

    Suisanzoshoku 44 (2) 169-175 1996年

  95. ステロイドの経口投与がアカウニ稚ウニの成長に及ぼす影響

    鵜沼 辰哉, 山本 剛史, 秋山 敏男

    水産増殖 44 (1) 79-83 1996年

    出版者・発行元: 日本水産増殖学会

    DOI: 10.11233/aquaculturesci1953.44.79  

    ISSN:0371-4217

    eISSN:2185-0194

    詳細を見る 詳細を閉じる

    テストステロン, エストロン, エストラジオールー17β, エタノール (対照区) を4.55×10-6g steroid/g飼料 (湿重量) の濃度で添加した4種類のウニ用精製飼料を調製して体重0.7gのアカウニ稚ウニを29日間給餌飼育し, 成長に及ぼす影響を調べた。エストロン添加区では飼育終了時の体重が対照区と比較して有意に増大し, 摂餌率・飼料効率は4試験区のうちで最も高かった。テストステロン添加区の体重も対照区よりやや上昇したものの, 有意差はなかった。エストラジオール-17βは成長・摂餌率・飼料効率に対して効果が認められなかった。

  96. The effect of combined use of several alternative protein sources in fingerling rainbow trout diets

    T Yamamoto, T Unuma, T Akiyama

    FISHERIES SCIENCE 61 (6) 915-920 1995年12月

    DOI: 10.2331/fishsci.61.915  

    ISSN:0919-9268

  97. COMBINATIONAL USE OF MALT PROTEIN FLOUR AND SOYBEAN-MEAL AS ALTERNATIVE PROTEIN-SOURCES OF FISH-MEAL IN FINGERLING RAINBOW-TROUT DIETS

    T AKIYAMA, T UNUMA, T YAMAMOTO, P MARCOULI, S KISHI

    FISHERIES SCIENCE 61 (5) 828-832 1995年10月

    DOI: 10.2331/fishsci.61.828  

    ISSN:0919-9268

  98. Utilization of malt protein flour in fingerling Japanese flounder diets

    Takeshi Yamamoto, Tatsuya Unuma, Toshio Akiyama

    Bulletin of National Research Institute of Aquaculture (24) 33-42 1995年3月

  99. Utilization of Malt Protein Flour in Fingerling Rainbow Trout Diets

    Takeshi Yamamoto, Panayiota A. Marcouli, Tatsuya Unuma, Toshio Akiyama

    FISHERIES SCIENCE 60 (4) 455-460 1994年

  100. Purification and Characterization of a Yolk Protein from the Scallop Ovary

    Makoto Osada, Katsuyoshi Mori, Tatsuya Unuma

    Nippon Suisan Gakkaishi 58 (12) 2283-2289 1992年

︎全件表示 ︎最初の5件までを表示

MISC 32

  1. ウニの生理学(第8回/最終回)光周期調節によるウニの成熟促進技術の開発とその展開

    石井正孝, 鵜沼辰哉

    養殖ビジネス = Aquaculture business 61 (7) 42-47 2024年6月

    ISSN: 2187-1442

  2. ウニの生理学(第6回)ウニを健全に育てる : 飼育成績を上げるための環境条件

    高橋 和寛, 鵜沼 辰哉

    養殖ビジネス = Aquaculture business 61 (2) 57-62 2024年2月

    出版者・発行元: 東京 : 緑書房

    ISSN: 2187-1442

  3. ウニの生理学(第5回)身が小さく色も悪い高齢ウニの短期養殖による商品化

    干川 裕, 鵜沼 辰哉

    養殖ビジネス = Aquaculture business 60 (13) 51-56 2023年12月

    出版者・発行元: 緑書房

    ISSN: 2187-1442

  4. ウニの生理学(第4回)北のバフンウニが苦くて食べられない理由とその解決策

    村田 裕子, 鵜沼 辰哉

    養殖ビジネス = Aquaculture business 60 (11) 62-67 2023年10月

    出版者・発行元: 緑書房

    ISSN: 2187-1442

  5. ウニの生理学(第3回)ウニのエサは低タンパク質で十分

    髙木 聖実, 鵜沼 辰哉

    養殖ビジネス = Aquaculture business 60 (9) 37-42 2023年8月

    出版者・発行元: 緑書房

    ISSN: 2187-1442

  6. ウニの生理学(第1回)ウニ漁業の現況と生理学的視点で見たウニの身

    鵜沼 辰哉

    養殖ビジネス = Aquaculture business 60 (2) 42-46 2023年2月

    出版者・発行元: 緑書房

    ISSN: 2187-1442

  7. Ⅱ-2. 成熟を抑制してウニを養殖する利点とその方法

    鵜沼 辰哉

    日本水産学会誌 87 (6) 692-692 2021年11月15日

    出版者・発行元: 公益社団法人 日本水産学会

    DOI: 10.2331/suisan.wa2867-7  

    ISSN: 0021-5392

    eISSN: 1349-998X

  8. Factors affecting the migration of juvenile chum salmon (Oncorhynchus keta) from the coast of Hokkaido to the Okhotsk Sea

    Tomonori Azumaya, Hiroshi Kuroda, Tatuya Unuma, Takashi Yokota, Shigehiko Urawa

    NPAFC Technical Report (17) 63-64 2021年

  9. 特性・流通面から見るウニ養殖 成功の鍵はエサの確保

    鵜沼 辰哉

    養殖ビジネス = Aquaculture business 56 (13) 4-8 2019年12月

    出版者・発行元: 緑書房

    ISSN: 2187-1442

  10. 流体モデルによるサケ稚魚の回遊シミュレーション—Migration routes of juvenile chum salmon simulated with a hydrodynamic model—特集 環境変動下におけるサケの持続可能な資源管理(2)

    東屋 知範, 黒田 寛, 高橋 大介, 鵜沼 辰哉, 横田 高士, 浦和 茂彦

    海洋と生物 = Aquabiology 40 (5) 453-458 2018年10月

    出版者・発行元: 生物研究社

    ISSN: 0285-4376

  11. Ⅳ-4. 流体モデルによるサケの回遊シミュレーション

    東屋 知範, 黒田 寛, 高橋 大介, 鵜沼 辰哉, 横田 高士, 浦和 茂彦

    日本水産学会誌 84 (5) 935-935 2018年9月15日

    出版者・発行元: 公益社団法人 日本水産学会

    DOI: 10.2331/suisan.wa2555-15  

    ISSN: 0021-5392

    eISSN: 1349-998X

  12. 3. 雑海藻を原料とした水産無脊椎動物用餌料

    鵜沼 辰哉

    日本水産学会誌 84 (1) 148-148 2018年1月

  13. Improving the food quality of sea urchins collected from barren grounds by short-term aquaculture under controlled temperature

    Tatsuya UNUMA, Yuko MURATA, Natsuki HASEGAWA, Sayumi SAWAGUCHI, Kazuhiro TAKAHASHI

    Bulletin of Fisheries Research Agency (40) 145-153 2015年

  14. ウニとナマコの種苗生産のための餌開発試験~スクリーニングに適した飼育法の活用~

    鵜沼 辰哉, 鬼塚 年弘, 酒井 勇一, 山野 恵祐

    育てる漁業 (469) 3-7 2015年

  15. 低温飼育による空ウニの品質改善

    鵜沼 辰哉

    北の海から (22) 2-2 2015年

  16. 研究グループ紹介 生産環境部資源増殖グループ

    鵜沼 辰哉

    北の海から (18) 4-4 2013年

  17. 研究室紹介 資源培養研究室

    鵜沼 辰哉

    北の海から (9) 4-4 2010年

  18. バリン、メチオニンおよびイノシン酸の国産ウニ類生殖巣における分布

    村田 裕子, 鵜沼 辰哉

    日本味と匂学会誌 = The Japanese journal of taste and smell research 16 (3) 445-446 2009年12月1日

    出版者・発行元: 日本味と匂学会

    ISSN: 1340-4806

  19. Relationship between gametogenesis and food quality in sea urchin gonads

    Tatsuya Unuma, Charles W. Walker

    Proceedings of the 36th U.S.-Japan Aquaculture Panel Symposium 45-54 2009年

  20. ウニの成熟機構

    鵜沼 辰哉

    磯焼け対策シリーズ2 磯焼けを起こすウニ−生態・利用から藻場回復まで− 211-217 2008年4月

  21. シラスウナギの人工生産へ向けて

    鵜沼 辰哉

    水産総合研究センター研究報告 = Bulletin of Fisheries Research Agency 別冊5 31-32 2006年3月

  22. ウナギサブチーム 個別飼育法によるニホンウナギの卵質判定—特別号:独立行政法人水産総合研究センター交付金プロジェクト 生態系保全型増養殖システム確立のための種苗生産・放流技術の開発--第1期成果 ; 生育・成熟等の生化学・分子生物学的解明に基づく種苗生産技術の高度化

    鵜沼 辰哉, 野村 和晴

    水産総合研究センター研究報告 = Bulletin of Fisheries Research Agency 別冊5 (-) 51-55 2006年3月

    出版者・発行元: 水産総合研究センター

    ISSN: 1346-9894

  23. クルマエビサブチーム クルマエビの卵の発達過程を探る--成熟卵を特徴づける物質の生合成過程の解明—特別号:独立行政法人水産総合研究センター交付金プロジェクト 生態系保全型増養殖システム確立のための種苗生産・放流技術の開発--第1期成果 ; 生育・成熟等の生化学・分子生物学的解明に基づく種苗生産技術の高度化

    山野 恵祐, 鵜沼 辰哉

    水産総合研究センター研究報告 = Bulletin of Fisheries Research Agency 別冊5 (-) 25-29 2006年3月

    出版者・発行元: 水産総合研究センター

    ISSN: 1346-9894

  24. A novel bitter amino acid, pulcherrimine isolated from sea urchin ovaries: relationship between maturation and their contents

    Yuko Murata, Ryuji Kuwahara, Oohara Ichiro, Masahito Yokoyama, Noriko U. Sata, Tatsuya Unuma, Masaki Kaneniwa, Tatsuya Yamamoto, Katashi Sugimoto, Hiroo Yamada, Yurie Kura

    Proceedings of the 32th U.S.-Japan Aquaculture Panel Symposium 158-164 2005年

  25. 第11回国際棘皮動物学会議

    鵜沼 辰哉

    日本水産學會誌 = Bulletin of the Japanese Society of Scientific Fisheries 70 (2) 217-217 2004年3月15日

    ISSN: 0021-5392

  26. Echinoferrin: a newly proposed name for a precursor to yolk protein in sea urchin

    Y Yokota, T Unuma, A. Moriyama

    Echinoderms: Munchen 79-81 2004年

  27. イタリアでのワークショップ「Sea Urchin Aquaculture」体験記

    鵜沼 辰哉

    養殖研ニュース (47) 17-19 2001年

  28. Acquisition and loss of potential for motility of spermatozoa of the Japanese eel Anguilla japonica

    Hiromi Ohta, Kazuo Ikeda, Hirohiko Kagawa, Hideki Tanaka, Tatsuya Unuma

    UJNR Technical Report (28) 77-81 1999年

  29. 二枚貝体内における炭素の流れ

    鵜沼 辰哉, 秋山 敏男

    養殖研ニュース (37) 6-8 1998年

  30. 魚の科学最先端(2)ウニの身入りを促進するには

    鵜沼 辰哉

    養殖 = Aquaculture magazine :つくる漁業の総合情報誌 34 (14) 93-95 1997年12月

    出版者・発行元: 緑書房

    ISSN: 0044-0671

  31. 二枚貝による栄養素の消化吸収率を求める

    鵜沼 辰哉

    養殖 = Aquaculture magazine :つくる漁業の総合情報誌 34 (4) 128-129 1997年

  32. 栄養物質貯蔵器官としての役割を果たすウニの生殖巣

    鵜沼 辰哉

    養殖研ニュース (30) 2-5 1995年

︎全件表示 ︎最初の5件までを表示

書籍等出版物 4

  1. 北海道マナマコ資源管理ガイドライン (北海道マナマコ資源管理技術開発共同研究機関 編著)

    鵜沼 辰哉, 佐野 稔, 長谷川 夏樹, 鬼塚 年弘

    北海道立総合研究機構稚内水産試験場 2014年

  2. 北海道マナマコ資源管理ガイドライン (北海道マナマコ資源管理技術開発共同研究機関 編著)

    鵜沼 辰哉, 長谷川 夏樹, 鬼塚 年弘

    北海道立総合研究機構稚内水産試験場 2014年

  3. ウニ学 (本川 達雄 編著)

    鵜沼 辰哉

    東海大学出版会 2009年3月

    ISBN: 9784486018100

  4. 磯焼けを起こすウニ : 生態・利用から藻場回復まで (藤田 大介, 町口 裕二, 桑原 久実 編著)

    鵜沼 辰哉

    成山堂書店 2008年4月

    ISBN: 9784425884117

共同研究・競争的資金等の研究課題 2

  1. 光周期調節による暖水系ウニの成熟制御手法の開発:増養殖現場での水温調節からの脱却

    鵜沼 辰哉

    2024年4月1日 ~ 2027年3月31日

  2. 地場種苗・健康診断・経営戦略でピンチを チャンスにかえるマガキ養殖システムの確立

    長谷川 夏樹, 宮田 勉, 若松 宏樹, 若松 宏樹, 神山 龍太郎, 山口 惠, 山口 慶子, 近田 靖子, 武山 悟, 藤原 正嗣, 奥村 宏征, 久野 正博, 栗山 功, 矢野 央樹, 谷田 圭亮, 安信 秀樹, 岡本 繁好, 増田 恵一, 五利江 重昭, 村上 倫哉, 永井 崇裕, 水野 健一郎, 黒田 麻美, 加川 真行, 若野 真, 柳川 建, 宇都宮 のぞみ, 森本 遼平, 木村 聡一郎, 岩野 英樹, 金澤 健, 白樫 真, 山田 英俊, 林 亨次, 濱田 真悠子, 﨑山 和昭, 圦本 達也, 松山 幸彦, 松本 才絵, 渡部 諭史, 石樋 由香, 坂見 知子, 馬久地 みゆき, 小林 正裕, 鬼塚 年弘, 伊藤 直樹, 北川 健斗, 鵜沼 辰哉, 東屋 知範, 伊藤 篤, 山崎 英樹, 前田 雪, 山田 充哉

    2018年4月 ~ 2021年3月