Details of the Researcher

PHOTO

Tatsuya Unuma
Section
Graduate School of Agricultural Science
Job title
Professor

Research History 11

  • 2023/04 - Present
    Tohoku University　Graduate School of Agricultural Science

  • 2021/04 - 2023/03
    国立研究開発法人水産研究・教育機構水産資源研究所　社会・生態系システム部

  • 2020/07 - 2021/03
    国立研究開発法人水産研究・教育機構水産技術研究所　沿岸生態システム部

  • 2016/04 - 2020/06
    国立研究開発法人水産研究・教育機構北海道区水産研究所　生産環境部

  • 2011/04 - 2016/03
    独立行政法人水産総合研究センター北海道区水産研究所　生産環境部

  • 2009/04 - 2011/03
    独立行政法人水産総合研究センター北海道区水産研究所　海区水産業研究部

  • 2006/04 - 2009/03
    独立行政法人水産総合研究センター日本海区水産研究所　業務推進部

  • 2003/04 - 2006/03
    独立行政法人水産総合研究センター養殖研究所　生産技術部

  • 2002/04 - 2003/03
    独立行政法人水産総合研究センター養殖研究所　繁殖部

  • 1998/10 - 2002/03
    水産庁養殖研究所　繁殖部

  • 1992/04 - 1998/09
    水産庁養殖研究所　栄養代謝部

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Education 2

  • Tohoku University　Graduate School of Agricultural Science

    1990/04 - 1992/03

  • Tohoku University

    1986/04 - 1990/03

Research Interests 4

  • 栄養生理

  • 繁殖生理

  • 魚類

  • 棘皮動物

Research Areas 1

  • Life sciences / Aquaculture /

Papers 100

  1. Changes in ATP-related compounds and freshness indices in sea urchin gonads during cold storage

    Yuko Murata, Takashi Matsuda, Hiroshi Hoshikawa, Tatsuya Unuma

    Fisheries Science　91　(4)　833-844　2025/04/26

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s12562-025-01876-4 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  2. The Sea Cucumber-Infecting Parasite Apostichocystis gudetama gen. nov. sp. nov. Expands Marine-Host-Specific Clade of Apicomplexans. International-journal

    Anthony M Bonacolta, Joana Krause-Massaguer, Tatsuya Unuma, Javier Del Campo

    The Journal of eukaryotic microbiology　72　(3)　e70013　2025

    DOI： 10.1111/jeu.70013 　

    More details Close

    Unknown ellipsoid bodies, later classified as apicomplexan cysts, are prevalent in the ovaries of Japanese sea cucumbers (Apostichopus japonicus), where they can lead to lower fecundity in infected individuals and adverse effects on wild populations as well as aquaculture efforts for this endangered species. Apicomplexans are widespread and essential to marine environments, where they can affect the health and fitness of host populations. We performed genomic sequencing of recovered cysts to gain more ecological and evolutionary information on this parasite. We recovered this apicomplexan's complete nuclear ribosomal RNA (rrn) operon, the entire mitochondrial genome, and a partial apicoplast (relic chloroplast) genome. The rrn operon phylogeny revealed this parasite as being closely related to coccidian-like parasites of marine fish (ichthyocolids) and cnidarians (corallicolids), while organelle phylogenomics hint at a closer relation to the protococcidian Eleutheroschizon. Using this new phylogenetic context and previous morphological descriptions, we describe this parasite as Apostichocystis gudetama gen. nov. sp. nov. Mining available microbiomes reveal the presence of Apostichocystis spp. beyond its host range, alluding to other potential hosts or cryptic, closely related lineages. Its phylogenetic placement has important implications concerning the evolution of parasitism within Apicomplexa and the divergence of a marine-host-specific clade of coccidian-like parasites.

  3. An investigation on developing an effective method for improving pigmentation in the muscle of sockeye salmon Oncorhynchus nerka

    山本剛史, 泉田大介, 矢野豊, 松成宏之, 伴真俊, 黒川忠英, 鵜沼辰哉

    日本水産学会誌　90　(5)　2024

    ISSN： 0021-5392

  4. Distribution pattern and habitat use of the protandrous shrimp Pandalus latirostris in relation to environmental characteristics in Akkeshi waters on the pacific coast of eastern Hokkaido, Japan

    Sayaka Ito, Toru Nakagawa, Norio Shirafuji, Takashi Yokota, Daisuke Izumida, Toshihiro Onitsuka, Hiroshi Kuroda, Tatsuya Unuma, Tsuyoshi Watanabe

    Regional Studies in Marine Science　67　2023/12/20

    DOI： 10.1016/j.rsma.2023.103198 　

    eISSN： 2352-4855

  5. Dietary protein requirement for somatic growth and gonad production in the sea urchin Strongylocentrotus intermedius at different life stages

    Satomi Takagi, Natsuki Hasegawa, Tsuyoshi Watanabe, Yuichi Sakai, Tatsuya Unuma

    AQUACULTURE　575　2023/10

    DOI： 10.1016/j.aquaculture.2023.739748 　

    ISSN： 0044-8486

    eISSN： 1873-5622

  6. Improving the gonad size and color of old sea urchins (Mesocentrotus nudus) by cultivation Peer-reviewed

    Hiroshi HOSHIKAWA, Akira SUGAWARA, Hiroya OKUMURA, Kanji NAKAJIMA, Tatsuya UNUMA

    SCIENTIFIC REPORTS OF HOKKAIDO FISHERIES RESEARCH INSTITUTES　(104)　1-11　2023/09

  7. Extension of immature stage of Hemicentrotus pulcherrimus ovary for reduction of the bitter compound (pulcherrimine) by water temperature manipulation

    Yuko Murata, Hiroshi Hoshikawa, Tomonori Kaneta, Satoshi Katayama, Tomonori Azumaya, Tatsuya Unuma

    Fisheries Science　2023

    DOI： 10.1007/s12562-023-01708-3 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  8. Repeated reaching of the harmful algal bloom of Karenia spp. around the Pacific shoreline of Kushiro, eastern Hokkaido, Japan, during autumn 2021

    Natsuki Hasegawa, Tsuyoshi Watanabe, Tatsuya Unuma, Takashi Yokota, Daisuke Izumida, Toru Nakagawa, Tadahide Kurokawa, Satomi Takagi, Tomonori Azumaya, Yukiko Taniuchi, Hiroshi Kuroda, Saho Kitatsuji, Kazuo Abe

    FISHERIES SCIENCE　88　(6)　787-803　2022/11

    DOI： 10.1007/s12562-022-01642-w 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  9. 光周期調節によるキタムラサキウニMesocentrotus nudusの成熟促進—Promoting gonadal maturation by photoperiod manipulation in the sea urchin Mesocentrotus nudus

    石井 正孝, 鵜沼 辰哉, 向阪 信一, 増田 篤稔, 村上 克介

    Eco-engineering　34　(3)　59-69　2022/07

    Publisher: 生態工学会

    ISSN： 1347-0485

  10. Controlling factors of large-scale harmful algal blooms with Karenia selliformis after record-breaking marine heatwaves

    Satomi Takagi, Hiroshi Kuroda, Natsuki Hasegawa, Tsuyoshi Watanabe, Tatsuya Unuma, Yukiko Taniuchi, Takashi Yokota, Daisuke Izumida, Toru Nakagawa, Tadahide Kurokawa, Tomonori Azumaya

    FRONTIERS IN MARINE SCIENCE　9　2022/07

    DOI： 10.3389/fmars.2022.939393 　

    eISSN： 2296-7745

  11. Accelerated photoperiod promotes gonadal maturation in the sea urchin Strongylocentrotus intermedius

    Masataka Ishii, Tatsuya Unuma, Akio Masadate, Hiroshi Hoshikawa, Kazuhiro Takahashi, Shinichi Kosaka, Atsunori Masuda, Katsusuke Murakami

    FISHERIES SCIENCE　88　(2)　299-310　2022/03

    DOI： 10.1007/s12562-022-01583-4 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  12. Modest Dietary Protein Requirement for Sea Urchin Gonad Production Demonstrated by Feeding Trials with Consideration of Protein Leaching

    Satomi Takagi, Kazuhiro Takahashi, Tomonori Kaneta, Akira Sugawara, Masanao Narita, Shinji Kato, Masaki Akino, Hirofumi Takeda, Natsuki Hasegawa, Yuji Machiguchi, Tatsuya Unuma

    AQUACULTURE NUTRITION　2022　2022/03

    DOI： 10.1155/2022/3140222 　

    ISSN： 1353-5773

    eISSN： 1365-2095

  13. Correction to: Effects of rearing temperature manipulation on oocyte maturation progress in Japanese eel (Fisheries Science, (2021), 87, 5, (681-691), 10.1007/s12562-021-01531-8)

    Toshiomi Tanaka, Shinji Adachi, Kazuharu Nomura, Hideki Tanaka, Tatsuya Unuma

    Fisheries Science　87　(5)　693-695　2021/09

    DOI： 10.1007/s12562-021-01537-2 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  14. Effects of rearing temperature manipulation on oocyte maturation progress in Japanese eel

    Toshiomi Tanaka, Shinji Adachi, Kazuharu Nomura, Hideki Tanaka, Tatsuya Unuma

    FISHERIES SCIENCE　87　(5)　681-691　2021/09

    DOI： 10.1007/s12562-021-01531-8 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  15. Accumulation of a bitter amino acid, pulcherrimine, in sea urchin ovaries and its relevance to the annual reproductive cycle

    Yuko Murata, Ken Saito, Katashi Sugimoto, Masato Kiyomoto, Tatsuya Unuma

    FISHERIES SCIENCE　87　(3)　371-381　2021/05

    DOI： 10.1007/s12562-021-01496-8 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  16. Coccidian Parasite in Sea Cucumber (Apostichopus japonicus) Ovaries

    Tatsuya Unuma, Noriko Tsuda, Yuichi Sakai, Takashi Kamaishi, Sayumi Sawaguchi, Naoki Itoh, Keisuke Yamano

    BIOLOGICAL BULLETIN　238　(1)　64-71　2020/02

    DOI： 10.1086/707807 　

    ISSN： 0006-3185

    eISSN： 1939-8697

  17. Gametogenesis in regular sea urchins: Structural, functional, and molecular/genomic biology

    Charles W. Walker, Michael P. Lesser, Tatsuya Unuma

    Developments in Aquaculture and Fisheries Science　43　29-50　2020

    DOI： 10.1016/B978-0-12-819570-3.00003-2 　

    ISSN： 0167-9309

  18. Compositions of extractive components in the testes and ovaries of various sea urchins: comparisons among species, sexes, and maturational status

    Yuko Murata, Hirokazu Yoshimura, Tatsuya Unuma

    FISHERIES SCIENCE　86　(1)　203-213　2020/01

    DOI： 10.1007/s12562-019-01388-y 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  19. Effects of taurine-enriched early live food (rotifer and Artemia nauplii) on the survival and growth of walleye pollock larvae

    Toru Nakagawa, Hiroyuki Matsunari, Takashi Yokota, Hiroshige Tanaka, Tetsuichiro Funamoto, Sayaka Ito, Takeshi Yamamoto, Tatsuya Unuma

    Aquaculture Science　67　(2)　157-170　2019/06

  20. A microplate incubation method for assessing egg quality of the barfin flounder: effects of well size and rearing medium on larval viability

    Tatsuya Unuma, Takashi Ichikawa, Sayumi Sawaguchi, Natsuki Hasegawa

    AQUACULTURE INTERNATIONAL　27　(1)　195-208　2019/02

    DOI： 10.1007/s10499-018-0316-5 　

    ISSN： 0967-6120

    eISSN： 1573-143X

  21. Enhancing the Commercial Quality of Edible Sea Urchin Gonads - Technologies Emphasizing Nutritive Phagocytes

    Charles W. Walker, S. A. Böttger, Tatsuya Unuma, Stephen A. Watts, Larry G. Harris, Addison L. Lawrence, Stephen D. Eddy

    Echinoderm Aquaculture　263-286　2015/11/06

    DOI： 10.1002/9781119005810.ch12 　

  22. Sea Urchin Aquaculture in Japan

    Tatsuya Unuma, Yuichi Sakai, Yukio Agatsuma, Takaaki Kayaba

    Echinoderm Aquaculture　75-126　2015/11/06

    DOI： 10.1002/9781119005810.ch5 　

  23. Dietary shifts in the juvenile sea urchin Strongylocentrotus intermedius associated with the development of digestive enzymes

    Toshihiro Onitsuka, Kentaro Niwa, Tatsuya Unuma, Yuya Umezu

    MARINE BIOLOGY　162　(4)　869-880　2015/04

    DOI： 10.1007/s00227-015-2630-z 　

    ISSN： 0025-3162

    eISSN： 1432-1793

  24. Fatty acid composition in sea cucumber Apostichopus japonicus fed with microbially degraded dietary sources

    Natsuki Hasegawa, Sayumi Sawaguchi, Masaharu Tokuda, Tatsuya Unuma

    AQUACULTURE RESEARCH　45　(12)　2021-2031　2014/11

    DOI： 10.1111/are.12149 　

    ISSN： 1355-557X

    eISSN： 1365-2109

  25. VARIATION IN MANILA CLAM (RUDITAPES PHILIPPINARUM) FECUNDITY IN EASTERN HOKKAIDO, JAPAN

    Natsuki Hasegawa, Sayumi Sawaguchi, Tatsuya Unuma, Toshihiro Onitsuka, Masami Hamaguchi

    JOURNAL OF SHELLFISH RESEARCH　33　(3)　739-746　2014/11

    DOI： 10.2983/035.033.0308 　

    ISSN： 0730-8000

    eISSN： 1943-6319

  26. Effects of diet and temperature on post-settlement growth and survival of the short-spined sea urchin Strongylocentrotus intermedius

    Toshihiro Onitsuka, Tatsuya Unuma, Tomohiko Kawamura

    AQUACULTURE RESEARCH　45　(1)　1-15　2013/12

    DOI： 10.1111/j.1365-2109.2012.03198.x 　

    ISSN： 1355-557X

    eISSN： 1365-2109

  27. Post-ovulatory oocyte aging induces spontaneous occurrence of polyploids and mosaics in artificial fertilization of Japanese eel, Anguilla japonica

    Kazuharu Nomura, Yukako Takeda, Tatsuya Unuma, Kagayaki Morishima, Hideki Tanaka, Katsutoshi Arai, Hiromi Ohta

    AQUACULTURE　404　15-21　2013/08

    DOI： 10.1016/j.aquaculture.2013.04.016 　

    ISSN： 0044-8486

  28. Sea urchin gametogenesis - Structural, functional and molecular/genomic biology

    Charles W. Walker, M. P. Lesser, Tatsuya Unuma

    Developments in Aquaculture and Fisheries Science　38　25-43　2013

    DOI： 10.1016/B978-0-12-396491-5.00003-4 　

    ISSN： 0167-9309

  29. Flocculent Feed for the Sea Cucumber Apostichopus japonicus Containing Diatomite and Zeolite

    Unuma Tatsuya, Noguchi Kohsuke, Sawaguchi Sayumi, Hasegawa Natsuki, Machiguchi Yuji

    Aquaculture Science　61　(4)　331-339　2013

    Publisher: Japanese Society for Aquaculture Science

    DOI： 10.11233/aquaculturesci.61.331 　

    ISSN： 0371-4217

    eISSN： 2185-0194

    More details Close

    A flocculent feed for the sea cucumber Aposticopus japonicus was developed. Mixture of powdered algae and commercial sea cucumber feed was added with 2.4 fold of inorganic matter (diatomite and zeolite) and gelatinized using calcium chloride with the alginate contained in the powdered algae as a binder, which led to a loosely aggregated mass of feed. In a 29-day feeding trial using juvenile A. japonicus of 7.8 mm body length, the flocculent test diet showed a significantly higher survival rate and specific growth rate than the powdered algae and the commercial sea cucumber feed. In a 42-day feeding trial using adult A. japonicus of 96 g body weight, the test diet resulted in significantly higher specific growth rate than two types of commercial sea cucumber feeds. Gonad index and histological observations of gonads suggested that oogenesis advanced more in females fed the test diet compared to those fed the other diets. These results indicate that the flocculent test diet is effective for rearing both juveniles and broodstock of A. japonicus.

  30. Optimum temperature of rearing water during artificial induction of ovulation in Japanese eel

    Tatsuya Unuma, Sayumi Sawaguchi, Natsuki Hasegawa, Noriko Tsuda, Toshiomi Tanaka, Kazuharu Nomura, Hideki Tanaka

    AQUACULTURE　358　216-223　2012/08

    DOI： 10.1016/j.aquaculture.2012.07.004 　

    ISSN： 0044-8486

  31. Fusion of lipid droplets in Japanese eel oocytes: Stage classification and its use as a biomarker for induction of final oocyte maturation and ovulation

    Tatsuya Unuma, Natsuki Hasegawa, Sayumi Sawaguchi, Toshiomi Tanaka, Takahiro Matsubara, Kazuharu Nomura, Hideki Tanaka

    AQUACULTURE　322　142-148　2011/12

    DOI： 10.1016/j.aquaculture.2011.10.001 　

    ISSN： 0044-8486

  32. Accumulation of the Major Yolk Protein and Zinc in the Agametogenic Sea Urchin Gonad

    Tatsuya Unuma, Sayumi Sawaguchi, Keisuke Yamano, Hiromi Ohta

    BIOLOGICAL BULLETIN　221　(2)　227-237　2011/10

    DOI： 10.1086/BBLv221n2p227 　

    ISSN： 0006-3185

    eISSN： 1939-8697

  33. Teratogenic Effects of the Chemicals Used for Assessing Egg Quality of Japanese Eel with Tissue Culture Microplates

    Unuma Tatsuya, Kurokawa Tadahide, Tokuda Masaharu, Nomura Kazuharu, Tanaka Hideki

    Aquaculture Science　59　(2)　307-313　2011

    Publisher: Japanese Society for Aquaculture Science

    DOI： 10.11233/aquaculturesci.59.307 　

    ISSN： 0371-4217

    eISSN： 2185-0194

    More details Close

    The individual rearing method using tissue culture microplates is useful for assessing fertility, hatchability, and larval survival in the Japanese eel Anguilla japonica. However, the rearing water needs to contain chemicals to suppress surface tension-related death that occurs upon hatching and antibiotics to prevent bacterial proliferation. To apply this method for assessing the occurrence of morphological deformities, it is essential to ensure that these additives do not induce malformations. Fertilized eggs were individually stocked in 48-well plates and reared until completion of yolk absorption. Polyethylene glycol 6,000 (PEG 6,000), bovine serum albumin (BSA), and an antibiotic solution containing penicillin G potassium (200,000 IU/ml) and streptomycin sulfate (0.2 g/ml) were added to the rearing water at various concentrations. PEG and BSA fully suppressed surface tension-related death at 1μg/ml and above without induction of dose-dependent malformations. The antibiotic solution fully prevented bacterial proliferation at 0.25μl/ml and above and did not induce dose-dependent malformations at 1μl/ml and below. However, it tended to increase the spinal curvature at 2.5μl/ml and above. We conclude that the individual rearing method can be applied to assess morphological deformities in eel larvae at the yolk absorption stage with minimum use of these additives within their effective concentrations.

  34. A genetic linkage map of the Japanese eel (Anguilla japonica) based on AFLP and microsatellite markers

    Kazuharu Nomura, Akiyuki Ozaki, Kagayaki Morishima, Yukio Yoshikawa, Hideki Tanaka, Tatsuya Unuma, Hiromi Ohta, Katsutoshi Arai

    AQUACULTURE　310　(3-4)　329-342　2011/01

    DOI： 10.1016/j.aquaculture.2010.11.006 　

    ISSN： 0044-8486

    eISSN： 1873-5622

  35. The role of the major yolk protein in sea urchin reproduction and its relevance to aquaculture

    T. Unuma, C. W. Walker

    ECHINODERMS: DURHAM　437-444　2010

  36. Molecular characterization of the major yolk protein of the Japanese common sea cucumber (Apostichopus japonicus) and its expression profile during ovarian development

    Atushi Fujiwara, Tatsuya Unuma, Kaoru Ohno, Keisuke Yamano

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY　155　(1)　34-40　2010/01

    DOI： 10.1016/j.cbpa.2009.09.002 　

    ISSN： 1095-6433

    eISSN： 1531-4332

  37. The Sea Urchin Major Yolk Protein Is Synthesized Mainly in the Gut Inner Epithelium and the Gonadal Nutritive Phagocytes Before and During Gametogenesis

    Tatsuya Unuma, Akifumi Nakamura, Keisuke Yamano, Yukio Yokota

    MOLECULAR REPRODUCTION AND DEVELOPMENT　77　(1)　59-68　2010/01

    DOI： 10.1002/mrd.21103 　

    ISSN： 1040-452X

    eISSN： 1098-2795

  38. Vitamin contents of eggs that produce larvae showing a high survival rate in the Japanese eel Anguilla japonica

    Hirofumi Furuita, Tatsuya Unuma, Kazuharu Nomura, Hideki Tanaka, Tsuyoshi Sugita, Takeshi Yamamoto

    AQUACULTURE RESEARCH　40　(11)　1270-1278　2009/07

    DOI： 10.1111/j.1365-2109.2009.02225.x 　

    ISSN： 1355-557X

  39. Vitamin content and quality of eggs produced by broodstock injected with vitamins C and E during artificial maturation in Japanese eel Anguilla japonica

    H. Furuita, T. Ishida, T. Suzuki, T. Unuma, T. Kurokawa, T. Sugita, T. Yamamoto

    AQUACULTURE　289　(3-4)　334-339　2009/04

    DOI： 10.1016/j.aquaculture.2009.01.032 　

    ISSN： 0044-8486

  40. The Major Yolk Protein is Synthesized in the Digestive Tract and Secreted Into the Body Cavities in Sea Urchin Larvae

    Tatsuya Unuma, Kooichi Konishi, Masato Kiyomoto, Valeria Matranga, Keisuke Yamano, Hiromi Ohta, Yukio Yokota

    MOLECULAR REPRODUCTION AND DEVELOPMENT　76　(2)　142-150　2009/02

    DOI： 10.1002/mrd.20939 　

    ISSN： 1040-452X

    eISSN： 1098-2795

  41. Exogastrulation and interference with the expression of major yolk protein by estrogens administered to sea urchins. International-journal

    Masato Kiyomoto, Ayumi Kikuchi, Seiko Morinaga, Tatuya Unuma, Yukio Yokota

    Cell biology and toxicology　24　(6)　611-20　2008/12

    DOI： 10.1007/s10565-008-9073-y 　

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    Although estrogens have been detected in some echinoderm species, their role is not clearly understood; so we examined the effects of estrogens administered to sea urchin embryos and larvae. A typical malformation was exogastrulation, induced by the exposure to ethynylestradiol (EER) in a defined period of 12 h from 12 h after fertilization (HAF). Morphogenesis for gastrulation was delayed in the treated embryos: protrusion of the archenteron started at 30 HAF when gastrulation had already finished in normal embryos. Exogastrulation induced by EER was cancelled by the antiestrogen chemical, ICI182,780. Feeding larvae were less sensitive to estrogens than those in early embryogenesis and, at certain concentrations, developed without abnormal morphology. The effect of estrogens was examined at the level of gene expression of the major yolk protein (MYP). MYP expression started during the larval stage and was suppressed by estrone at the six-armed stage, but not by beta-estradiol, and in later stage larvae, the expression was not affected by treatment with either estrogen. Estrogens affect sea urchins in the early stage of embryogenesis, leading to abnormal morphogenesis and interference with gene expression.

  42. Influence of Water Temperature on Morphological Deformities in Cultured Larvae of Japanese Eel, Anguilla japonica, at Completion of Yolk Resorption

    Tadahide Kurokawa, Takuma Okamoto, Koichiro Gen, Susumu Uji, Koji Murashita, Tatsuya Unuma, Kazuharu Nomura, Hajime Matsubara, Shin-Kwon Kim, Hiromi Ohta, Hideki Tanaka

    JOURNAL OF THE WORLD AQUACULTURE SOCIETY　39　(6)　726-735　2008/12

    DOI： 10.1111/j.1749-7345.2008.00208.x 　

    ISSN： 0893-8849

  43. Occurrence of spontaneously spermiating eels in captivity

    Hajime Matsubara, Hideki Tanaka, Kazuharu Nomura, Tohru Kobayashi, Koji Murashita, Tadahide Kurokawa, Tatsuya Unuma, Shin-Kwon Kim, Mark P. Lokman, Takahiro Matsubara, Hirohiko Kagawa, Hiromi Ohta

    CYBIUM　32　(2)　174-175　2008/07

    ISSN： 0399-0974

  44. Zinc-binding property of the major yolk protein in the sea urchin - implications of its role as a zinc transporter for gametogenesis

    Tatsuya Unuma, Kazuo Ikeda, Keisuke Yamano, Akihiko Moriyama, Hiromi Ohta

    FEBS JOURNAL　274　(19)　4985-4998　2007/10

    DOI： 10.1111/j.1742-4658.2007.06014.x 　

    ISSN： 1742-464X

  45. Chapter 2 Gametogenesis and reproduction of sea urchins

    Charles W Walker, Tatsuya Unuma, Michael P Lesser

    Developments in Aquaculture and Fisheries Science　37　11-33　2007

    DOI： 10.1016/S0167-9309(07)80066-4 　

    ISSN： 0167-9309

  46. Lipid and fatty acid composition of eggs producing larvae with high survival rate in the Japanese eel

    H. Furuita, T. Unuma, K. Nomura, H. Tanaka, K. Okuzawa, T. Sugita, T. Yamamoto

    JOURNAL OF FISH BIOLOGY　69　(4)　1178-1189　2006/10

    DOI： 10.1111/j.1095-8649.2006.01196.x 　

    ISSN： 0022-1112

  47. Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families

    K Nomura, K Morishima, H Tanaka, T Unuma, K Okuzawa, H Ohta, K Arai

    AQUACULTURE　257　(1-4)　53-67　2006/06

    DOI： 10.1016/j.aquaculture.2006.03.011 　

    ISSN： 0044-8486

  48. Effects of ethynylestradiol and bisphenol A on the development of sea urchin embryos and juveniles

    M Kiyomoto, A Kikuchi, T Unuma, Y Yokota

    MARINE BIOLOGY　149　(1)　57-63　2006/04

    DOI： 10.1007/s00227-005-0208-x 　

    ISSN： 0025-3162

  49. Expressed sequence tags from eyestalk of kuruma prawn, Marsupenaeus japonicus. International-journal

    Keisuke Yamano, Tatsuya Unuma

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology　143　(2)　155-61　2006/02

    ISSN： 1095-6433

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    We analyzed the expressed sequence tags (ESTs) obtained from a cDNA library of the eyestalk of the kuruma prawn, Marsupenaeus japonicus, to examine gene expression profile with special focus on female reproduction. The assembly of 1988 ESTs created 136 contigs from 738 ESTs; however 1250 ESTs remained singletons. Significant similarities (blast score > or = 50 bits) to the DNA sequences in the databank were found for only 16.7% of the 1386 sequences (136 contigs plus 1250 singletons), suggesting that the eyestalk library contains many unknown genes. Ribosomal RNA and mitochondrial respiration enzymes with significant similarities were found abundantly in the ESTs, whereas genes related to maturation or endocrine systems were scarce. Three ESTs were assumed to encode novel eyestalk hormones with marked similarities to pigment-dispersing hormone, molt-inhibiting hormone and crustacean hyperglycemic hormone. Sequences encoding a product highly homologous to farnesoic acid O-methyltransferase, an enzyme that produces methyl farnesoate, were also found.

  50. Microsatellite genotypes in triploid Japanese eel (Anguilla japonica) induced by heat shock Peer-reviewed

    K Nomura, H Kagawa, H Tanaka, K Okuzawa, T Unuma, K Arai

    AQUACULTURE　247　(1-4)　20-20　2005/06

    ISSN： 0044-8486

  51. Relationship between egg specific gravity and egg quality in the Japanese eel, Anguilla japonica

    T Unuma, S Kondo, H Tanaka, H Kagawa, K Nomura, H Ohta

    AQUACULTURE　246　(1-4)　493-500　2005/05

    DOI： 10.1016/j.aquaculture.2005.01.011 　

    ISSN： 0044-8486

    eISSN： 1873-5622

  52. The first success of glass eel production in the world: basic biology on fish reproduction advances new applied technology in aquaculture

    Hirohiko Kagawa, Hideki Tanaka, Hiromi Ohta, Tatsuya Unuma, Kazuharu Nomura

    FISH PHYSIOLOGY AND BIOCHEMISTRY　31　(2-3)　193-199　2005/04

    DOI： 10.1007/s10695-006-0024-3 　

    ISSN： 0920-1742

    eISSN： 1573-5168

  53. Cathepsin C transcripts are differentially expressed in the final stages of oocyte maturation in kuruma prawn Marsupenaeus japonicus. International-journal

    Gao-Feng Qiu, Keisuke Yamano, Tatsuya Unuma

    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology　140　(2)　171-81　2005/02

    ISSN： 1096-4959

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    To elucidate the molecular mechanism of oocyte maturation in the kuruma prawn (Marsupenaeus japonicus), subtractive suppression hybridization (SSH) was initially used to identify novel up-regulated genes during the final stages of oocyte maturation, followed by evaluation of the differential expression profile by macroarray and quantitative real-time RT-PCR analyses. The cathepsin C (dipeptidyl peptidase I) gene was thus found to exhibit a significantly higher expression around the onset of cortical rod (CR) formation (early CR stage, appearance of round CRs), progress to a higher mRNA level until the middle CR stage (elongation of CRs), then rapidly revert to a low expression level at the late CR stage (occurrence of germinal vesicle breakdown, GVBD), as also observed at the non-CR stage (previtellogenesis and vitellogenesis). In situ hybridization analyses revealed that the sites of the expression of cathepsin C transcripts in the ovary were distributed in both oocyte and follicle cells, particularly at the early CR stage. A full-length cDNA sequence of this stage-specific gene was subsequently determined by rapid amplification of the cDNA 3' and 5' ends (3' and 5' RACE). The deduced amino acid sequence of the 230-residue mature peptide shared 67-70% identity to the known cathepsin C in mammals. Western blot analysis showed that expression of procathepsin C protein was exclusively at CR stages. The storage site of procathepsin C protein was localized in CRs as revealed by immunohistochemical analysis. This is the first report on the full-length cDNA sequence of cathepsin C and a demonstration of its involvement in the final stages of oocyte maturation in crustacean species.

  54. Determination of the rates of fertilization, hatching and larval survival in the Japanese eel, Anguilla japonica, using tissue culture microplates

    T Unuma, S Kondo, H Tanaka, H Kagawa, K Nomura, H Ohta

    AQUACULTURE　241　(1-4)　345-356　2004/11

    DOI： 10.1016/j.aquaculture.2004.08.005 　

    ISSN： 0044-8486

  55. Molecular cloning and ovarian expression profiles of thrombospondin, a major component of cortical rods in mature oocytes of penaeid shrimp, Marsupenaeus japonicus. International-journal

    Keisuke Yamano, Gao-Feng Qiu, Tatsuya Unuma

    Biology of reproduction　70　(6)　1670-8　2004/06

    ISSN： 0006-3363

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    In penaeid shrimp, cortical rods (CRs) are formed in peripheral crypts of the oocyte after completion of yolk accumulation; subsequently the CRs are utilized as a source of jelly materials that surround fertilized eggs. In our previous study, of five major components, three CR proteins displayed quite similar immunological characteristics. In this study, cDNA sequences and developmental expression profiles at both transcriptional and protein levels were examined to elucidate the molecular characteristics of CR proteins and the process of CR formation. Sequencing cDNAs exhibited the presence of three related forms that have identical sequences except for the loss of 246 and 369 bp in medium and short forms, respectively, suggesting that a single gene generates three transcriptional variants corresponding to the three CR proteins. Their deduced amino acid sequences revealed similarities to those of extracellular matrix proteins in a thrombospondin (TSP) 3,4/cartilage oligomeric protein family, and thereby the CR proteins were designated mjTSP. Semiquantitative analysis by real-time polymerase chain reaction revealed the presence of mjTSP transcripts, at similar levels, in immature, vitellogenic, and mature ovaries. Furthermore, in situ hybridization localized the majority of transcripts in previtellogenic oocytes in ovaries at all developmental stages. By the Western blot, on the other hand, mjTSP proteins were undetectable in immature ovaries but became obvious at the early vitellogenic stage. The immunosignals were enhanced during vitellogenic stages and maintained a high intensity in mature ovaries. Thus, transcription, translation of mjTSP, and formation of the CR structure occurred at different stages of ovarian development.

  56. Development of endocrine system regulating exocrine pancreas and estimation of feeding and digestive ability in Japanese eel larvae

    T Kurokawa, N Iinuma, T Unuma, H Tanaka, H Kagawa, H Ohta, T Suzuki

    AQUACULTURE　234　(1-4)　513-525　2004/05

    DOI： 10.1016/j.aquaculture.2003.12.002 　

    ISSN： 0044-8486

  57. Induction of triploidy by heat shock in the Japanese eel Anguilla japonica

    K Nomura, J Nakajima, H Ohta, H Kagawa, H Tanaka, T Unuma, K Yamauchi, K Arai

    FISHERIES SCIENCE　70　(2)　247-255　2004/04

    DOI： 10.1111/j.1444-2906.2003.00798.x 　

    ISSN： 0919-9268

  58. Occurrence of the sea urchin with pulcherrimine, a novel bitter amino acid

    Y Murata, M Yokoyama, T Unuma, NU Sata, R Kuwahara, M Kaneniwa, Oohara, I

    MORE EFFICIENT UTILIZATION OF FISH AND FISHERIES PRODUCTS　42　289-300　2004

    ISSN： 0167-4501

  59. Care and maintenance of adult echinoderms International-journal

    S Anne Böttger, Charles W Walker, Tatsuya Unuma

    Methods in cell biology　74　17-38　2004

    ISSN： 0091-679X

  60. Immunological characterization of cortical rod proteins of kuruma prawn, Marsupenaeus japonicus. International-journal

    Keisuke Yamano, Hisatake Seto, Gao-Feng Qiu, Tatsuya Unuma

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology　136　(2)　371-377　2003/10

    ISSN： 1095-6433

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    The penaeid shrimp develops cortical rods (CRs) in the oocyte after completing yolk accumulation during ovarian maturation. In this study, CRs from the mature ovary of the kuruma prawn (Marsupenaeus japonicus) were isolated and their proteins were characterized. Under reducing conditions of SDS-PAGE, the CR demonstrated five major bands of approximately 210, 150, 140, 130 and 30 kDa. Of 32 clones of monoclonal antibodies raised against the CR proteins, 21 were found to be immunoreactive by Western blotting, demonstrating 150, 140 and 130 kDa proteins simultaneously. This result suggested that the proteins were post-translational or post-transcriptional products from the same gene, or that they originated from the same gene family. CRs were identified by immunohistochemical analysis by 31 clones of monoclonal antibodies in paraffin sections and by 26 clones in cryostatic ones. Most of the antibodies also stained the yolk in both developing and mature oocytes, suggesting that CR proteins accumulated in developing oocytes, then assembled to form the CR structure.

  61. Cleavage site of a major yolk protein (MYP) determined by cDNA isolation and amino acid sequencing in sea urchin, Hemicentrotus pulcherrimus. International-journal

    Yukio Yokota, Tatsuya Unuma, Akihiko Moriyama, Keisuke Yamano

    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology　135　(1)　71-81　2003/05

    ISSN： 1096-4959

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    The overall sequence of cDNA encoding vitellogenin (Vg), a precursor to major yolk protein (MYP), of Hemicentrotus pulcherrimus was determined. Its nucleotide sequence has an open reading frame of 4041 bp encoding 1346 amino acids. The amino acid sequence showed little similarity to other Vgs in vertebrates, insects or nematodes, but resembled members of the vertebrate and invertebrate transferrin family. The N-terminal amino acid sequence of the protein fragments dominant in the later embryonic stage was analyzed in order to determine the cleavage site of MYP. Determination of the cleavage site in MYP and analysis of MYP proteolysis in vitro suggested that MYP has a specific molecular shape to permit its proteolytic fragmentation at a definite site. The functional region of transferrin in MYP is conserved after proteolytic processing. Considering these results and those from other work, the protein called sea urchin Vg is not a true Vg. Therefore, a new name, echinoferrin, is proposed for this protein.

  62. Role of prostaglandin in the control of ovulation in the Japanese eel Anguilla japonica

    H Kagawa, H Tanaka, T Unuma, H Ohta, K Gen, K Okuzawa

    FISHERIES SCIENCE　69　(2)　234-241　2003/04

    DOI： 10.1046/j.1444-2906.2003.00613.x 　

    ISSN： 0919-9268

  63. Changes in fertility of rainbow trout eggs retained in coelom

    T Azuma, H Ohta, S Oda, K Muto, T Yada, T Unuma

    FISHERIES SCIENCE　69　(1)　131-136　2003/02

    DOI： 10.1046/j.1444-2906.2003.00597.x 　

    ISSN： 0919-9268

  64. Induction of sperm maturation

    H Ohta, T Unuma

    EEL BIOLOGY　415-423　2003

    DOI： 10.1007/978-4-431-65907-5_28 　

  65. Occurrence of spontaneous polyploids from the eggs obtained by artificial induction of maturation in the Japanese eel (Anguilla japonica)

    H Ohta, Y Higashimoto, S Koga, T Unuma, K Nomura, H Tanaka, H Kagawa, K Arai

    FISH PHYSIOLOGY AND BIOCHEMISTRY　28　(1-4)　517-518　2003

    DOI： 10.1023/B:FISH.0000030648.83531.0c 　

    ISSN： 0920-1742

  66. The first production of glass eel in captivity: fish reproductive physiology facilitates great progress in aquaculture

    H Tanaka, H Kagawa, H Ohta, T Unuma, K Nomura

    FISH PHYSIOLOGY AND BIOCHEMISTRY　28　(1-4)　493-497　2003

    DOI： 10.1023/B:FISH.0000030638.56031.ed 　

    ISSN： 0920-1742

  67. Biochemical composition of eggs in relation to egg quality in the Japanese eel, Anguilla japonica

    H Furuita, H Ohta, T Unuma, H Tanaka, H Kagawa, N Suzuki, T Yamamoto

    FISH PHYSIOLOGY AND BIOCHEMISTRY　29　(1)　37-46　2003

    DOI： 10.1023/B:FISH.0000035897.58924.9d 　

    ISSN： 0920-1742

    eISSN： 1573-5168

  68. Quantitative changes in yolk protein and other components in the ovary and testis of the sea urchin Pseudocentrotus depressus. International-journal

    T Unuma, T Yamamoto, T Akiyama, M Shiraishi, H Ohta

    Journal of Experimental Biology　206　(2)　365-372　2003/01

    ISSN： 0022-0949

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    Both male and female sea urchins accumulate the major yolk protein (MYP; the most abundant yolk granule protein in sea urchin eggs) in the nutritive phagocytes of immature gonads before gametogenesis. In this study, quantitative changes in MYP as well as in other biochemical components in the ovary and testis were examined in the course of gametogenesis in Pseudocentrotus depressus. Before gametogenesis, both the ovary and testis contained large quantities of proteins, lipids and polysaccharides. MYP reached about 80% of total protein in both sexes. In the testis, MYP decreased rapidly as spermatogenesis proceeded, and the fully mature testis contained little MYP; the levels of lipids and polysaccharides also decreased. In contrast, the levels of nucleic acids and proteins other than MYP increased markedly. In the ovary, MYP decreased gradually as oogenesis proceeded, and the fully mature ovary contained less than half of the initial amount of MYP. Polysaccharides also decreased, whereas proteins other than MYP increased. These results, taken together with those from other studies, suggest that MYP serves as a protein reserve that accumulates before gametogenesis and is used as material for synthesizing new substances constituting gametes in both male and female sea urchins.

  69. Expression of pancreatic enzyme genes during the early larval stage of Japanese eel Anguilla japonica

    T Kurokawa, T Suzuki, H Ohta, H Kagawa, H Tanaka, T Unuma

    FISHERIES SCIENCE　68　(4)　736-744　2002/08

    DOI： 10.1046/j.1444-2906.2002.00487.x 　

    ISSN： 0919-9268

  70. Seasonal changes of bitterness and pulcherrimine content in gonads of green sea urchin Hemicentrotus pulcherrimus at Iwaki in Fukushima Prefecture

    Y Murata, M Yokoyama, T Unuma, NU Sata, R Kuwahara, M Kaneniwa

    FISHERIES SCIENCE　68　(1)　184-189　2002/02

    DOI： 10.1046/j.1444-2906.2002.00406.x 　

    ISSN： 0919-9268

  71. Gonadal growth and its relationship to aquaculture in sea urchins

    Tatsuya Unuma

    The Sea Urchin : from Basic Biology to Aquaculture　115-127　2002

  72. Control by the environmental concentration of ions of the potential for motility in Japanese eel spermatozoa

    H Ohta, H Kagawa, H Tanaka, T Unuma

    AQUACULTURE　198　(3-4)　339-351　2001/07

    DOI： 10.1016/S0044-8486(00)00597-4 　

    ISSN： 0044-8486

  73. Effects of bicarbonate ions and pH on acquisition and maintenance of potential for motility in ayu, Plecoglossus altivelis Temminck et Schlegel (Osmeridae), spermatozoa

    H Ohta, T Unuma, M Tsuji, M Yoshioka, M Kashiwagi

    AQUACULTURE RESEARCH　32　(5)　385-392　2001/05

    DOI： 10.1046/j.1365-2109.2001.00571.x 　

    ISSN： 1355-557X

  74. Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depresses

    T Unuma, H Okamoto, K Konishi, H Ohta, K Mori

    ZOOLOGICAL SCIENCE　18　(4)　559-565　2001/05

    DOI： 10.2108/zsj.18.559 　

    ISSN： 0289-0003

  75. Optimum protein level in a purified diet for young red sea urchin Pseudocentrotus depressus

    T Akiyama, T Unuma, T Yamamoto

    FISHERIES SCIENCE　67　(2)　361-363　2001/04

    DOI： 10.1046/j.1444-2906.2001.00252.x 　

    ISSN： 0919-9268

  76. Cryopreservation of the sperm of the Japanese bitterling

    H Ohta, K Kawamura, T Unuma, Y Takegoshi

    JOURNAL OF FISH BIOLOGY　58　(3)　670-681　2001/03

    DOI： 10.1006/jfbi.2000.1477 　

    ISSN： 0022-1112

  77. Reproduction of sea urchins

    C. W. Walker, T. Unuma, N. A. McGinn, L. M. Harrington, M. P. Lesser

    Developments in Aquaculture and Fisheries Science　32　(C)　5-26　2001

    DOI： 10.1016/S0167-9309(01)80003-X 　

    ISSN： 0167-9309

  78. Nutritional Evaluation of Brown Algae Arame Eisenia bicyclis Sampled at Different Seasons and Places as a Feed for Red Sea Urchin Pseudocentrotus depressus

    AKIYAMA Toshio, YAMAMOTO Takeshe, UNUMA Tatsuya, SHINOHARA Naoya

    Aquaculture Science　49　(4)　475-482　2001

    Publisher: Japanese Society for Aquaculture Science

    DOI： 10.11233/aquaculturesci1953.49.475 　

    ISSN： 0371-4217

    eISSN： 2185-0194

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    Protein, amino acid and energy contents of arame Eisenia bicyclis, which is one of main feeds for red sea urchin, were examined at different seasons, geographic places, and sections of a leaf to evaluate the nutritional values.<BR>Composition of the algae leaves seasonally changed. The highest values in the ratio of dry matter, crude protein and energy contents were obtained in July to October, whose values were 29-32%, 3-4% and 360-370 kcal/100g dry matter, respectively. Whereas the lowest values were 14%, 1.9%, and 270 kcal in February. The crude protein contents, however, did not reach the optimum protein level for red sea urchin diet throughout the whole year. The quantity and balance of essential amino acids in the leaves were influenced by season rather than geographic difference between Mie and Fukuoka. Moreover, there were some nutritional differences among the segments on a leaf, and the budding segment had much better essential amino acid balance than the shedding segments but was obviously lower in the protein content.<BR>The protein nutritional values of Eisenia were inferior to those of casein and wakame Undaria pinnatifida, mainly due to the low level of arginine. Consequently, supplemental feeding of higharginine containing diet may be effective to promote the growth when a main feed is Eisenia for urchin culture.

  79. Voluntary intake of diets with varying digestible energy contents and energy sources, by juvenile rainbow trout Oncorhynchus mykiss, using self-feeders

    T Yamamoto, T Shima, T Unuma, M Shiraishi, T Akiyama, M Tabata

    FISHERIES SCIENCE　66　(3)　528-534　2000/06

    DOI： 10.1046/j.1444-2906.2000.00083.x 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  80. The influence of dietary protein sources on tissue free amino acid levels of fingerling rainbow trout

    T Yamamoto, T Unuma, T Akiyama

    FISHERIES SCIENCE　66　(2)　310-320　2000/04

    DOI： 10.1046/j.1444-2906.2000.00049.x 　

    ISSN： 0919-9268

  81. The influence of dietary protein and fat levels on tissue free amino acid levels of fingerling rainbow trout (Oncorhynchus mykiss)

    T Yamamoto, T Unuma, T Akiyama

    AQUACULTURE　182　(3-4)　353-372　2000/02

    DOI： 10.1016/S0044-8486(99)00277-X 　

    ISSN： 0044-8486

  82. Diluents for Cool Storage of Milt and for Artificial Fertilization in the Amago Salmon Oncorhynchus masou ishikawae

    Ohta Hiromi, Unuma Tatsuya, Nagoya Hiroyuki

    NIPPON SUISAN GAKKAISHI　66　(1)　88-96　2000

    Publisher: The Japanese Society of Fisheries Science

    DOI： 10.2331/suisan.66.88 　 10.1007/s12562-015-0899-y_references_DOI_GgopNbe2jc83C6vMS9U039e5uks 　

    ISSN： 0021-5392

    eISSN： 1349-998X

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    Diluents for cool storage of milt and for artificial fertilization which induce a high percentage of motility of stored spermatozoa were examined. Milt was stored in artificial seminal plasma (ASP; NaCl+KCl=140mM, CaCl2 1.6mM, MgCl2 1.0mM and NaHCO3 10mM buffered with 20mM TAPS-NaOH at pH 8.0) of various KCl concentrations (0 to 50mM) at 4°C for 10 days. The sperm motility was examined after dilution with DW (buffered with 10mM TAPS at pH 8.0) or 120mM NaHCO3. Increasing the potassium concentration of ASP to 30mM increased the percent motility. The spermatozoa stored for two days in Ca-free ASP showed remarkably decreased motility. The motility of stored spermatozoa is also influenced by the pH of ASP. A 120mM NaHCO3 solution induced a higher percentage and longer duration of motility of stored spermatozoa than DW. A 120mM NaCl solution and an artificial coelomic fluid also induced a higher percent motility; no significant differences in motility were detected among these three solutions. Adding 5mM IBMX (3-isobutyl-1-methylxanthine) to 120mM NaHCO3 or 120mM NaCl failed to increase the percent motility. Changing NaHCO3 concentration from 60 to 180mM induced no significant difference of percent motility either 5 to 7 sec or 15 to 17 sec after the dilution.

  83. Effect of Steroids on Gonadal Growth and Gametogenesis in the Juvenile Red Sea Urchin Pseudocentrotus depressus. International-journal

    T Unuma, T Yamamoto, T Akiyama

    The Biological bulletin　196　(2)　199-204　1999/04

    DOI： 10.2307/1542565 　

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    Red sea urchins, 10 months old, were fed for 30 days on a casein-based diet containing progesterone, androstenedione, testosterone, estrone, or estradiol-17{beta}. The mean gonad indices of male animals in the androstenedione-and the estrone-treated groups were significantly higher than those in the control group, suggesting that these steroids promote gonadal growth in male animals. Histological observations indicated that spermatogenesis in the estrone-treated group was also promoted compared to that in the control group. In contrast, female urchins were not obviously affected by the steroid-treated diets, probably because yearling female P. depressus are not otherwise ready to carry out gametogenesis. We conclude that androstenedione, estrone, and possibly their derivatives, are involved in the reproduction of male P. depressus.

  84. Postprandial changes in plasma free amino acid concentrations of rainbow trout fed diets containing different protein sources

    T Yamamoto, T Unuma, T Akiyama

    FISHERIES SCIENCE　64　(3)　474-481　1998/06

    DOI： 10.2331/fishsci.64.474 　

    ISSN： 0919-9268

  85. Apparent and true availabilities of amino acids from several protein sources for fingerling rainbow trout, common carp, and red sea bream

    T Yamamoto, A Akimoto, S Kishi, T Unuma, T Akiyama

    FISHERIES SCIENCE　64　(3)　448-458　1998/06

    DOI： 10.2331/fishsci.64.448 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  86. Apparent availabilities of amino acids from several protein sources for fingerling Japanese flounder

    Takeshi Yamamoto, Tatsuya Unuma, Toshio Akiyama

    Bulletin of National Research Institute of Aquaculture　(27)　27-35　1998/03

  87. A Protein Identical to the Yolk Protein Is Stored in the Testis in Male Red Sea Urchin, Pseudocentrotus depressus. International-journal

    T Unuma, T Suzuki, T Kurokawa, T Yamamoto, T Akiyama

    The Biological bulletin　194　(1)　92-97　1998/02

    DOI： 10.2307/1542517 　

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    Female sea urchins store the major yolk protein (MYP) in ovarian nutritive phagocytes before vitellogenesis. Using immunological procedures, we detected MYP in the testicular nutritive phagocytes of Pseudocentrotus depressus, the red sea urchin, and then compared the distribution of MYP between sexes during gametogenesis. MYP was purified from unfertilized eggs by ion exchange chromatography (Q Sepharose) and gel filtration (Superdex 200), and an antiserum (anti-MYP) was raised against MYP. Immunoblot analysis demonstrated that immature testes, as well as ovaries, contained a large quantity of MYP. Immunohistochemistry showed that MYP was distributed in the nutritive phagocytes occupying the follicular lumen in both males and females. In both sexes, as gametogenesis proceeded, the nutritive phagocytes degenerated and the gonadal lumen filled with gametes. MYP accumulated in ripe ova as a yolk protein in the mature ovary. In contrast, MYP was not detected in mature testes, because stored spermatozoa did not react with anti-MYP. We conclude that in male P. depressus, MYP is stored in the testicular nutritive phagocytes and utilized as the nutrient source for spermatogenesis.

  88. Apparent availabilities of amino acids and minerals from several protein sources for fingerling rainbow trout

    T Yamamoto, K Ikeda, T Unuma, T Akiyama

    FISHERIES SCIENCE　63　(6)　995-1001　1997/12

    DOI： 10.2331/fishsci.63.995 　

    ISSN： 0919-9268

    eISSN： 1444-2906

  89. An evaluation of amino acid sources and binders in semipurified diet for red sea urchin Pseudocentrotus depressus

    T Akiyama, T Unuma, T Yamamoto, H Furuita, K Konishi

    FISHERIES SCIENCE　63　(6)　881-886　1997/12

    DOI： 10.2331/fishsci.63.881 　

    ISSN： 0919-9268

  90. Effect of dietary tryptophan on maturation of ayu Plecoglossus altivelis

    T Akiyama, M Shiraishi, T Yamamoto, T Unuma

    FISHERIES SCIENCE　62　(5)　776-782　1996/10

    DOI： 10.2331/fishsci.62.776 　

    ISSN： 0919-9268

  91. Utilization of malt protein flour in fingerling carp diets

    T Yamamoto, T Unuma, T Akiyama, S Kishi

    FISHERIES SCIENCE　62　(5)　783-789　1996/10

    DOI： 10.2331/fishsci.62.783 　

    ISSN： 0919-9268

  92. 飼料へのクロレラエキス添加が早熟系アマゴの成熟に及ぼす影響

    鵜沼, 辰哉, 秋山, 敏男, 山本, 剛史, 新間, 脩子

    養殖研究所研究報告 = Bulletin of National Research Institute of Aquaculture　(25)　55-58　1996/03

    Publisher: 水産庁養殖研究所

    ISSN： 0389-5858

  93. Utilization of malt protein flour in the diets for fingerling red sea bream

    T Yamamoto, T Unuma, T Akiyama, S Kishi

    FISHERIES SCIENCE　62　(1)　59-63　1996/02

    DOI： 10.2331/fishsci.62.59 　

    ISSN： 0919-9268

  94. Seasonal Changes in Gonads of Cultured and Wild Red Sea Urchin, Pseudocentrotus depressus

    Tatsuya UNUMA, Kooichi KONISHI, Hirofumi FURUITA, Takeshi YAMAMOTO, Toshio AKIYAMA

    Suisanzoshoku　44　(2)　169-175　1996

  95. Effects of Oral Administration of Steroids on the Growth of Juvenile Sea Urchin, Pseudocentrotus depressus.

    UNUMA Tatsuya, YAMAMOTO Takeshi, AKIYAMA Toshio

    Aquaculture Science　44　(1)　79-83　1996

    Publisher: Japanese Society for Aquaculture Science

    DOI： 10.11233/aquaculturesci1953.44.79 　

    ISSN： 0371-4217

    eISSN： 2185-0194

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    Three purified diets each containing testosterone, estrone, or estradiol at the level of 4.55×10-6 g steroid/g wet diet and an ethanol-treated diet as a control were fed to juvenile sea urchin, Pseudocentrotus depressus for 29 days to examine their influence on growth and food utilization. The sea urchins treated with estrone showed significantly higher body weight than the control group (p < 0.05) . Food intake and feed efficiency in the estrone-treated group were the highest among the four groups. The growth of the sea urchins treated with testosterone was slightly higher but not significantly different from that of the control group. Estradiol had no effect on growth and food utilization of juvenile sea urchin.

  96. The effect of combined use of several alternative protein sources in fingerling rainbow trout diets

    T Yamamoto, T Unuma, T Akiyama

    FISHERIES SCIENCE　61　(6)　915-920　1995/12

    DOI： 10.2331/fishsci.61.915 　

    ISSN： 0919-9268

  97. COMBINATIONAL USE OF MALT PROTEIN FLOUR AND SOYBEAN-MEAL AS ALTERNATIVE PROTEIN-SOURCES OF FISH-MEAL IN FINGERLING RAINBOW-TROUT DIETS

    T AKIYAMA, T UNUMA, T YAMAMOTO, P MARCOULI, S KISHI

    FISHERIES SCIENCE　61　(5)　828-832　1995/10

    DOI： 10.2331/fishsci.61.828 　

    ISSN： 0919-9268

  98. Utilization of malt protein flour in fingerling Japanese flounder diets

    Takeshi Yamamoto, Tatsuya Unuma, Toshio Akiyama

    Bulletin of National Research Institute of Aquaculture　(24)　33-42　1995/03

  99. Utilization of Malt Protein Flour in Fingerling Rainbow Trout Diets

    Takeshi Yamamoto, Panayiota A. Marcouli, Tatsuya Unuma, Toshio Akiyama

    FISHERIES SCIENCE　60　(4)　455-460　1994

  100. Purification and Characterization of a Yolk Protein from the Scallop Ovary

    Makoto Osada, Katsuyoshi Mori, Tatsuya Unuma

    Nippon Suisan Gakkaishi　58　(12)　2283-2289　1992

Show all ︎Show first 5

Misc. 32

  1. ウニの生理学(第8回/最終回)光周期調節によるウニの成熟促進技術の開発とその展開

    石井正孝, 鵜沼辰哉

    養殖ビジネス = Aquaculture business　61　(7)　42-47　2024/06

    ISSN： 2187-1442

  2. ウニの生理学(第6回)ウニを健全に育てる : 飼育成績を上げるための環境条件

    高橋 和寛, 鵜沼 辰哉

    養殖ビジネス = Aquaculture business　61　(2)　57-62　2024/02

    Publisher: 東京 : 緑書房

    ISSN： 2187-1442

  3. ウニの生理学(第5回)身が小さく色も悪い高齢ウニの短期養殖による商品化

    干川 裕, 鵜沼 辰哉

    養殖ビジネス = Aquaculture business　60　(13)　51-56　2023/12

    Publisher: 緑書房

    ISSN： 2187-1442

  4. ウニの生理学(第4回)北のバフンウニが苦くて食べられない理由とその解決策

    村田 裕子, 鵜沼 辰哉

    養殖ビジネス = Aquaculture business　60　(11)　62-67　2023/10

    Publisher: 緑書房

    ISSN： 2187-1442

  5. ウニの生理学(第3回)ウニのエサは低タンパク質で十分

    髙木 聖実, 鵜沼 辰哉

    養殖ビジネス = Aquaculture business　60　(9)　37-42　2023/08

    Publisher: 緑書房

    ISSN： 2187-1442

  6. ウニの生理学(第1回)ウニ漁業の現況と生理学的視点で見たウニの身

    鵜沼 辰哉

    養殖ビジネス = Aquaculture business　60　(2)　42-46　2023/02

    Publisher: 緑書房

    ISSN： 2187-1442

  7. Ⅱ-2. Suppressing maturation in sea urchin aquaculture: potential benefits and methods

    UNUMA TATSUYA

    NIPPON SUISAN GAKKAISHI　87　(6)　692-692　2021/11/15

    Publisher: The Japanese Society of Fisheries Science

    DOI： 10.2331/suisan.wa2867-7 　

    ISSN： 0021-5392

    eISSN： 1349-998X

  8. Factors affecting the migration of juvenile chum salmon (Oncorhynchus keta) from the coast of Hokkaido to the Okhotsk Sea

    Tomonori Azumaya, Hiroshi Kuroda, Tatuya Unuma, Takashi Yokota, Shigehiko Urawa

    NPAFC Technical Report　(17)　63-64　2021

  9. 特性・流通面から見るウニ養殖 成功の鍵はエサの確保

    鵜沼 辰哉

    養殖ビジネス = Aquaculture business　56　(13)　4-8　2019/12

    Publisher: 緑書房

    ISSN： 2187-1442

  10. 流体モデルによるサケ稚魚の回遊シミュレーション—Migration routes of juvenile chum salmon simulated with a hydrodynamic model—特集 環境変動下におけるサケの持続可能な資源管理(2)

    東屋 知範, 黒田 寛, 高橋 大介, 鵜沼 辰哉, 横田 高士, 浦和 茂彦

    海洋と生物 = Aquabiology　40　(5)　453-458　2018/10

    Publisher: 生物研究社

    ISSN： 0285-4376

  11. Ⅳ-4. Migration routes of juvenile chum salmon simulated with hydrodynamic model

    AZUMAYA TOMONORI, KURODA HIROSHI, TAKAHASHI DAISUKE, UNUMA TATSUYA, YOKOTA TAKASHI, URAWA SHIGEHIKO

    NIPPON SUISAN GAKKAISHI　84　(5)　935-935　2018/09/15

    Publisher: The Japanese Society of Fisheries Science

    DOI： 10.2331/suisan.wa2555-15 　

    ISSN： 0021-5392

    eISSN： 1349-998X

  12. 3. 雑海藻を原料とした水産無脊椎動物用餌料

    鵜沼 辰哉

    日本水産学会誌　84　(1)　148-148　2018/01

  13. Improving the food quality of sea urchins collected from barren grounds by short-term aquaculture under controlled temperature

    Tatsuya UNUMA, Yuko MURATA, Natsuki HASEGAWA, Sayumi SAWAGUCHI, Kazuhiro TAKAHASHI

    Bulletin of Fisheries Research Agency　(40)　145-153　2015

  14. ウニとナマコの種苗生産のための餌開発試験～スクリーニングに適した飼育法の活用～

    鵜沼 辰哉, 鬼塚 年弘, 酒井 勇一, 山野 恵祐

    育てる漁業　(469)　3-7　2015

  15. 低温飼育による空ウニの品質改善

    鵜沼 辰哉

    北の海から　(22)　2-2　2015

  16. 研究グループ紹介 生産環境部資源増殖グループ

    鵜沼 辰哉

    北の海から　(18)　4-4　2013

  17. 研究室紹介 資源培養研究室

    鵜沼 辰哉

    北の海から　(9)　4-4　2010

  18. Concentration of Val, Met and IMP in gonads of seven edible species of sea urchin in Japan

    MURATA Yuko, UNUMA Tatsuya

    The Japanese journal of taste and smell research　16　(3)　445-446　2009/12/01

    Publisher: The Japanese Association for the Study of Taste and Smell

    ISSN： 1340-4806

  19. Relationship between gametogenesis and food quality in sea urchin gonads

    Tatsuya Unuma, Charles W. Walker

    Proceedings of the 36th U.S.-Japan Aquaculture Panel Symposium　45-54　2009

  20. ウニの成熟機構

    鵜沼 辰哉

    磯焼け対策シリーズ2 磯焼けを起こすウニ−生態・利用から藻場回復まで−　211-217　2008/04

  21. シラスウナギの人工生産へ向けて

    鵜沼 辰哉

    水産総合研究センター研究報告 = Bulletin of Fisheries Research Agency　別冊5　31-32　2006/03

  22. ウナギサブチーム 個別飼育法によるニホンウナギの卵質判定—特別号:独立行政法人水産総合研究センター交付金プロジェクト 生態系保全型増養殖システム確立のための種苗生産・放流技術の開発--第1期成果 ; 生育・成熟等の生化学・分子生物学的解明に基づく種苗生産技術の高度化

    鵜沼 辰哉, 野村 和晴

    水産総合研究センター研究報告 = Bulletin of Fisheries Research Agency　別冊5　(-)　51-55　2006/03

    Publisher: 水産総合研究センター

    ISSN： 1346-9894

  23. クルマエビサブチーム クルマエビの卵の発達過程を探る--成熟卵を特徴づける物質の生合成過程の解明—特別号:独立行政法人水産総合研究センター交付金プロジェクト 生態系保全型増養殖システム確立のための種苗生産・放流技術の開発--第1期成果 ; 生育・成熟等の生化学・分子生物学的解明に基づく種苗生産技術の高度化

    山野 恵祐, 鵜沼 辰哉

    水産総合研究センター研究報告 = Bulletin of Fisheries Research Agency　別冊5　(-)　25-29　2006/03

    Publisher: 水産総合研究センター

    ISSN： 1346-9894

  24. A novel bitter amino acid, pulcherrimine isolated from sea urchin ovaries: relationship between maturation and their contents

    Yuko Murata, Ryuji Kuwahara, Oohara Ichiro, Masahito Yokoyama, Noriko U. Sata, Tatsuya Unuma, Masaki Kaneniwa, Tatsuya Yamamoto, Katashi Sugimoto, Hiroo Yamada, Yurie Kura

    Proceedings of the 32th U.S.-Japan Aquaculture Panel Symposium　158-164　2005

  25. 11th International Echinoderm Conference

    70　(2)　217-217　2004/03/15

    ISSN： 0021-5392

  26. Echinoferrin: a newly proposed name for a precursor to yolk protein in sea urchin

    Y Yokota, T Unuma, A. Moriyama

    Echinoderms: Munchen　79-81　2004

  27. イタリアでのワークショップ「Sea Urchin Aquaculture」体験記

    鵜沼 辰哉

    養殖研ニュース　(47)　17-19　2001

  28. Acquisition and loss of potential for motility of spermatozoa of the Japanese eel Anguilla japonica

    Hiromi Ohta, Kazuo Ikeda, Hirohiko Kagawa, Hideki Tanaka, Tatsuya Unuma

    UJNR Technical Report　(28)　77-81　1999

  29. 二枚貝体内における炭素の流れ

    鵜沼 辰哉, 秋山 敏男

    養殖研ニュース　(37)　6-8　1998

  30. 魚の科学最先端(2)ウニの身入りを促進するには

    鵜沼 辰哉

    養殖 = Aquaculture magazine :つくる漁業の総合情報誌　34　(14)　93-95　1997/12

    Publisher: 緑書房

    ISSN： 0044-0671

  31. 二枚貝による栄養素の消化吸収率を求める

    鵜沼 辰哉

    養殖 = Aquaculture magazine :つくる漁業の総合情報誌　34　(4)　128-129　1997

  32. 栄養物質貯蔵器官としての役割を果たすウニの生殖巣

    鵜沼 辰哉

    養殖研ニュース　(30)　2-5　1995

Show all ︎Show first 5

Books and Other Publications 4

  1. 北海道マナマコ資源管理ガイドライン (北海道マナマコ資源管理技術開発共同研究機関 編著)

    鵜沼 辰哉, 佐野 稔, 長谷川 夏樹, 鬼塚 年弘

    北海道立総合研究機構稚内水産試験場　2014

  2. 北海道マナマコ資源管理ガイドライン (北海道マナマコ資源管理技術開発共同研究機関 編著)

    鵜沼 辰哉, 長谷川 夏樹, 鬼塚 年弘

    北海道立総合研究機構稚内水産試験場　2014

  3. ウニ学 (本川 達雄 編著)

    鵜沼 辰哉

    東海大学出版会　2009/03

    ISBN: 9784486018100

  4. 磯焼けを起こすウニ : 生態・利用から藻場回復まで (藤田 大介, 町口 裕二, 桑原 久実 編著)

    鵜沼 辰哉

    成山堂書店　2008/04

    ISBN: 9784425884117

Research Projects 2

  1. 光周期調節による暖水系ウニの成熟制御手法の開発：増養殖現場での水温調節からの脱却

    鵜沼 辰哉

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2024/04/01 - 2027/03/31

  2. 地場種苗・健康診断・経営戦略でピンチを チャンスにかえるマガキ養殖システムの確立

    長谷川 夏樹, 宮田 勉, 若松 宏樹, 若松 宏樹, 神山 龍太郎, 山口 惠, 山口 慶子, 近田 靖子, 武山 悟, 藤原 正嗣, 奥村 宏征, 久野 正博, 栗山 功, 矢野 央樹, 谷田 圭亮, 安信 秀樹, 岡本 繁好, 増田 恵一, 五利江 重昭, 村上 倫哉, 永井 崇裕, 水野 健一郎, 黒田 麻美, 加川 真行, 若野 真, 柳川 建, 宇都宮 のぞみ, 森本 遼平, 木村 聡一郎, 岩野 英樹, 金澤 健, 白樫 真, 山田 英俊, 林 亨次, 濱田 真悠子, 﨑山 和昭, 圦本 達也, 松山 幸彦, 松本 才絵, 渡部 諭史, 石樋 由香, 坂見 知子, 馬久地 みゆき, 小林 正裕, 鬼塚 年弘, 伊藤 直樹, 北川 健斗, 鵜沼 辰哉, 東屋 知範, 伊藤 篤, 山崎 英樹, 前田 雪, 山田 充哉

    Offer Organization: 生物系

    System: イノベーション創出強化研究推進事業（開発研究ステージ）

    Institution: 水産機構、道総研釧水試、三重水研、兵庫水技セ、 広島水海技セ、大分水研、厚岸町、東大院、ケアシェル（株）

    2018/04 - 2021/03