東北大学研究者紹介

研究者詳細

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タナカ　テツヤ

田仲　哲也

Tetsuya Tanaka

所属

大学院農学研究科　生物生産科学専攻　動物生命科学講座（動物微生物学分野）

職名

教授

学位

博士（獣医学）（岐阜大学）
修士（農学）（帯広畜産大学）

researchmap

https://researchmap.jp/read0065424

J-GLOBAL ID

200901067973854530

プロフィール

岐阜大学大学院連合獣医学研究科博士課程修了。博士（獣医学）。微生物化学研究所、帯広畜産大学原虫病分子免疫研究センター、北海道大学大学院農学研究科、ブリュッセル自由大学分子生物医科学研究所を経て、鹿児島大学農学部助教授、鹿児島大学共同獣医学部教授、2024年より現職。現在の専門分野は、節足動物を含めた病原体のタンパク質機能化学。主な論文に「マダニ、原虫、ウイルス、細菌、ミルクタンパク質」など169報。趣味は本屋巡り、食べ飲み歩き、山歩き。

経歴 9

2026年～継続中

東北大学食と農免疫国際教育研究センター　教授（兼任）
2024年～継続中

東北大学　大学院農学研究科･農学部　教授
2021年～継続中

フィリピン大学ロスバニョス校　客員教授（兼任）
2002年～ 2025年

帯広畜産大学原虫病研究センター共同研究員（兼任）
2018年～ 2024年

鹿児島大学　共同獣医学部　教授
2013年～ 2024年

今村学園ライセンスアカデミー　講師
2013年～ 2018年

鹿児島大学　共同獣医学部　准教授
2006年～ 2012年

鹿児島大学農学部獣医学科准教授
2006年～ 2012年

鹿児島大学フロンティアサンエンス研究推進センター准教授（兼任）

︎全件表示 ︎最初の5件までを表示

学歴 3

岐阜大学大学院　連合獣医学研究科　基礎獣医学専攻

1993年～ 1997年
帯広畜産大学大学院　畜産学研究科　家畜生産科学科専攻

1991年～ 1993年
帯広畜産大学　畜産学部　家畜生産科学科

1987年～ 1991年

委員歴 1

日本獣医学会、日本獣医寄生虫学会、日本寄生虫学会　評議員

所属学協会 4

日本獣医寄生虫学会
日本分子生物学会
日本寄生虫学会
日本獣医学会

研究キーワード 3

感染症学
節足動物分子工学
タンパク質機能化学

研究分野 3

ライフサイエンス / 寄生虫学 /
環境・農学 / 昆虫科学 /
ライフサイエンス / 獣医学 /

論文 187

Molecular survey and phylogenetic analysis of Borrelia theileri, Rickettsia aeschlimannii, Mycoplasma ovis, and Mycoplasma wenyonii in sheep and goats from southern Egypt. 国際誌査読有り

Hassan Y A H Mahmoud, Ahmed M Soliman, Wafa Abdullah I Al-Megrin, Hind Alzaylaee, Tetsuya Tanaka, Walaa F A Emeish

Scientific reports　2025年12月29日

DOI： 10.1038/s41598-025-32438-4 　
Molecular detection and phylogenetic characterization of Borrelia theileri and a putative novel Borrelia genotype in Hyalomma dromedarii ticks infesting camels in southern Egypt. 国際誌査読有り

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Samah Mohamed, Tatsuro Hifumi, Kyoko Tsukiyama-Kohara, Tetsuya Tanaka

Comparative immunology, microbiology and infectious diseases　124　102403-102403　2025年9月5日

DOI： 10.1016/j.cimid.2025.102403 　

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Ticks are important vectors of various pathogens affecting human and animal health globally. In Egypt, camels are frequently infested with Hyalomma dromedarii, yet their role in harboring and transmitting tick-borne pathogens remains poorly understood. This study aimed to molecularly detect and characterize Borrelia species in H. dromedarii ticks collected from camels in three cities in southern Egypt: Esna, Draw, and Edfu. Of 300 ticks analyzed using PCR targeting the 16S rRNA and flaB genes, 21.3 % (64/300) tested positive for Borrelia species. The highest prevalence was recorded in Edfu (37 %), followed by Draw (19 %) and Esna (13.3 %). Sequencing of the flaB gene revealed two distinct genetic groups. Phylogenetic analysis demonstrated that one group clustered closely with Borrelia theileri, while the second formed a distinct clade within the relapsing fever group of Borrelia species, showing sufficient genetic divergence to suggest a putative novel genotype or locally adapted strain. This clade was supported by high bootstrap values (99 %) and showed no close relation to previously described species. To our knowledge, this study represents the first detection of B. theileri in camel-associated ticks in Egypt. These findings expand the known genetic diversity of Borrelia species in North Africa and highlight the need for further studies to assess the pathogenic potential and epidemiological significance of these strains in animal and human populations.
First detection of Rickettsia japonica in Hyalomma dromedarii ticks infesting camels from Egypt: A call for enhanced surveillance of tick-borne pathogens. 国際誌査読有り

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Samah Mohamed, Tatsuro Hifumi, Kyoko Tsukiyama-Kohara, Tetsuya Tanaka

Medical and veterinary entomology　40　(1)　163-169　2025年9月4日

DOI： 10.1111/mve.70013 　

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Tick-borne rickettsiosis has posed a significant threat to Egypt, with various pathogenic Rickettsia species being reported. In this study, 134 ticks were collected from camels in Esna City, Luxor, Egypt and all were identified as Hyalomma dromedarii through both morphological and molecular techniques. Using specific primers targeting the citrate synthase (gltA), outer membrane protein A (ompA) and 17 kD antigen (17 kDa) genes, Rickettsia japonica was detected via conventional and nested PCR assays. Remarkably, two samples tested positive for R. japonica across all three genes, indicating a prevalence of 1.5%. Phylogenetic analysis based on the gltA, ompA and 17 kDa genes confirmed the presence of R. japonica within H. dromedarii ticks. This is the first reported detection of R. japonica in Egypt. These findings highlight the urgent need to investigate the origin and spread of R. japonica in the country and underscore the importance of continuous surveillance and data collection on lesser-known pathogens circulating in ticks as part of Egypt's public health efforts to manage tick-borne diseases.
First detection of Colpodella spp. in Rhipicephalus annulatus and molecular characterization of piroplasmids in southern Egypt. 国際誌査読有り

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Samah Mohamed, Tatsuro Hifumi, Kyoko Tsukiyama-Kohara, Tetsuya Tanaka

Frontiers in veterinary science　12　1617204-1617204　2025年7月5日

DOI： 10.3389/fvets.2025.1617204 　

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Piroplasmosis is a tick-borne disease that can affect livestock, particularly cattle. Its causative pathogens are intracellular apicomplexan parasites belonging to the order Piroplasmida. We recently identified one such emergent pathogen (Colpodella spp.) in ticks infesting camel in Egypt. Accordingly, we aimed to ascertain the presence of hemoprotozoan parasites in ticks infesting cattle. We removed ticks from household cattle during veterinary examinations, and submitted them for morphological examination and PCR analyses for species identification. Ticks and hemoprotozoan species obtained from tick samples were also evaluated using BLAST analysis, followed by confirmatory phylogenetic analyses. The collected ticks were identified as belonging to three species: Hyalomma dromedarii, Hyalomma marginatum, and Rhipicephalus annulatus. Phylogenetic analysis based on the 16S rRNA gene revealed that these ticks were clustered with those of the relevant species previously documented in Egypt. Molecular analysis targeting the 18S rRNA gene revealed Colpodella spp., the second such report in Egypt and the first in R. annulatus ticks infesting cattle. The Colpodella minimum infection rate (MIR) was 2.3% (per sample of pooled ticks from a single bovine host). Furthermore, Babesia bovis, Theileria. annulata, and Theileria orientalis were detected with MIRs of 3.5%, 4.7%, and 0.39%, respectively. In the phylogenetic analysis, each detected pathogen clustered with its corresponding species. Specifically, the Colpodella spp. were grouped with Colpodella spp. previously detected in Rhipicephalus microplus, Rhipicephalus haemaphysaloides ticks, and humans in China (accession numbers MH208620, MH208621, and GQ411073), and H. dromedarii ticks infesting camel in southern Egypt (accession numbers LC775361 and LC775361). We confirmed the detection of B. bovis and T. annulata through PCR assays with specific primers targeting the spherical body protein-4 gene and the major merozoite surface antigen gene, respectively. The detection of Colpodella spp. in ticks infesting cattle highlights the need for ongoing surveillance of this parasites. Both cattle and camels may serve as sentinel species, emphasizing the importance of monitoring these livestock for emerging parasites.
Vaccinomics-based identification of immunodominant epitopes in the tick-borne encephalitis virus polyprotein for multi-epitope vaccine development 査読有り

Sudais Rahman, Da-Wei Huang, Mohibullah Shah, Mashal M. Almutairi, Iram Liaqat, Tetsuya Tanaka, Chien-Chin Chen, Sher Bahadar, Abid Ali

Computational and Structural Biotechnology Reports　2　100047-100047　2025年7月1日
出版者・発行元： Elsevier BV
DOI： 10.1016/j.csbr.2025.100047 　

ISSN：2950-3639
Molecular prevalence and phylogenetic characterization of Francisella-like endosymbionts in ticks infesting camels and cattle from Southern Egypt. 国際誌査読有り

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Samah Mohamed, Tatsuro Hifumi, Tetsuya Tanaka

Microbial pathogenesis　205　107639-107639　2025年4月25日

DOI： 10.1016/j.micpath.2025.107639 　

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Ticks serve as vectors for numerous pathogens affecting human and animal health and harbor various microorganisms, including Francisella-like endosymbionts (FLEs), whose potential pathogenicity remains uncertain. In Egypt, data on FLEs, particularly in Southern Egypt, are limited, despite the importance of tick-borne pathogen surveillance in livestock. This study represents the first report of FLEs in Southern Egypt and the first detection of FLEs in Rhipicephalus annulatus in Egypt. A total of 726 ticks infesting cattle and camels were collected from various locations in Southern Egypt. Ticks were identified using both morphological and molecular techniques based on the 16S rRNA gene, and FLE detection was performed using PCR assays targeting a fragment of the Francisella 16S rRNA gene. Francisella DNA was detected in 60.1 % (288/479) of the examined ticks, with the highest prevalence in Hyalomma marginatum (100 %, 4/4), followed by Hyalomma dromedarii (74.8 %, 243/325), and R. annulatus (27.3 %, 41/150). Sequencing of 19 positive samples revealed that H. dromedarii (n = 13), H. marginatum (n = 2), and R. annulatus (n = 4) carried FLE sequences with 99-100 % similarity to strains previously identified in ticks worldwide. Given the high prevalence of FLEs in livestock-associated ticks, further research should focus on characterizing these strains, assessing their role in tick-borne pathogen ecology, and evaluating potential implications for human and animal health.
Molecular detection of Rickettsia aeschlimannii, Borrelia theileri, and Francisella-like endosymbionts in Camelus dromedarius and dogs in Luxor, Egypt. 国際誌査読有り

Hassan Y A H Mahmoud, Ahmed M Soliman, Moshera S Shahat, Ali A Hroobi, Ali H Alghamdi, Abdullah M Almotayri, Tetsuya Tanaka, Walaa F A Emeish

Scientific reports　15　(1)　12872-12872　2025年4月15日

DOI： 10.1038/s41598-025-91530-x 　

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Vector-borne bacterial pathogens can cause disease in a range of animals, including dromedary camels and dogs, but epidemiological and molecular studies on these pathogens are scarce in southern Egypt. In this study, we screened camels and dogs in southern Egypt (Luxor) for vector-borne bacterial pathogens, with molecular analysis of 200 blood samples collected from camels and dogs in the region. The Rickettsia aeschlimannii gltA gene was detected in 5% (5/100) of camel blood samples and 1% (1/100) of dog blood samples. This study is the first report of Rickettsia aeschlimannii in camel blood in southern Egypt. Additionally, the 16S rRNA gene of a Francisella-like endosymbiont was detected in both camel and dog blood for the first time, with infection rates of 2% (2/100) in camels and 2% (2/100) in dogs. In dog blood, the Borrelia theileri flaB gene was detected for the first time in southern Egypt at a positivity rate of 5% (5/100). Neither Coxiella nor Bartonella species were detected in this study. In southern Egypt, Rickettsia aeschlimannii, Borrelia theileri, and Francisella-like endosymbionts were detected in camels and dogs, providing valuable information about their infection rate and these findings contribute to a better understanding of their transmission dynamics.
First detection and molecular characterization of Mycoplasma species in camels, cattle, buffalo, and their associated ticks from southern Egypt. 国際誌査読有り

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Samah Mohamed, Tatsuro Hifumi, Abdelrahman A Rady, Alsagher O Ali, Walaa F A Emeish, Tetsuya Tanaka

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases　105750-105750　2025年4月8日

DOI： 10.1016/j.meegid.2025.105750 　

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Mycoplasma species are wall-less bacteria that infect multiple mammalian orders, including livestock, and are associated with economically significant diseases. In Egypt, molecular data on the presence and diversity of Mycoplasma spp. in camels, cattle, buffalo, and their associated ticks remain limited, despite their potential impact on livestock health and productivity. This study aimed to detect and genetically characterize Mycoplasma spp. in cattle, buffalo, camels, and their associated ticks in southern Egypt. A total of 571 samples (384 ticks, 50 buffalo, 68 cattle, and 69 camels) were screened using PCR targeting the 16S rRNA gene, followed by phylogenetic analysis of representative amplicons. Hyalomma dromedarii, Hyalomma marginatum, and Rhipicephalus annulatus were the tick species collected. Overall, Mycoplasma DNA was detected in 10.3 % of the samples. Mycoplasma ovis-like was identified in 2.9 % of camel blood, 9.4 % of H. dromedarii, 75 % of H. marginatum, and 13.8 % of R. annulatus ticks. Candidatus Mycoplasma haematovis was found in 0.4 % of H. dromedarii, and Mycoplasma wenyonii-like in 13.2 % of cattle and 4 % of buffalo blood. Remarkably, this is the first global detection of M. ovis in camel blood, as well as the detection of M. ovis-like and Ca. M. haematovis in ticks circulating on camels. This study also reports the first detection of M. wenyonii-like in bovines in Egypt and documents M. ovis-like in three tick species parasitizing cattle. The genetic similarities of Mycoplasma sequences from ticks and livestock suggest that these arthropods may serve as potential vectors. These findings highlight the need for additional studies on the zoonotic potential, infectivity, and vector competence of Mycoplasma spp. in Egypt.
Prediction of potential drug targets and key inhibitors (ZINC67974679, ZINC67982856, and ZINC05668040) against Rickettsia felis using integrated computational approaches. 国際誌査読有り

Sudais Rahman, Hsien Liu, Mohibuallah Shah, Mashal M Almutairi, Iram Liaqat, Tetsuya Tanaka, Chien-Chin Chen, Abdulaziz Alouffi, Abid Ali

Frontiers in veterinary science　11　1507496-1507496　2025年1月16日

DOI： 10.3389/fvets.2024.1507496 　

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Rickettsia felis, responsible for flea-borne spotted fever, is a rising zoonotic pathogen posing an increasing global threat due to its expanding geographical distribution. The rise in antibiotic-resistant strains of this pathogen underscores the urgent need for new therapeutic interventions. This study employed a comprehensive subtractive proteomics analysis of the R. felis proteome, aiming to identify essential, non-host homologous, and pathogen-specific proteins, which were subsequently evaluated as potential new drug targets. These findings offer valuable insights into the development of therapeutic strategies against rickettsiosis. The analysis revealed 343 proteins that are non-homologous to the host, including 108 essential proteins, 25 unique metabolic pathways, and 11 distinct proteins. Out of these, 10 proteins were druggable in which two associated with virulence, and one related to resistance (succinate dehydrogenase). Through a rigorous screening process and extensive literature review, succinate dehydrogenase emerged as a promising drug target. Protein interaction partners for succinate dehydrogenase were identified using the STRING database. To further assess the functionality of succinate dehydrogenase, structure-based studies were conducted. Approximately 18,000 ZINC compounds were screened, leading to the finding of six potential inhibitors: ZINC67847806, ZINC67982856, ZINC67974679, ZINC67895371, ZINC05668040, and ZINC05670149. Absorption, distribution, metabolism, excretion, and toxicity (ADMET) profiling confirmed that most compounds met the preferred pharmacokinetic properties, except for ZINC67895371 and ZINC67847806, which exhibited positive ames test results, and ZINC05670149, ZINC67895371, and ZINC67847806, showed hepatotoxicity. All compounds were found to be non-sensitizing to the skin. Based on these findings, further experimental validation of ZINC67974679, ZINC67982856, and ZINC05668040 is recommended.
Teratological abnormalities in hard ticks (Acari: Ixodidae) from Pakistan. 国際誌査読有り

Ejaz Akbar, Shafi Ullah, Mashal M Almutairi, Iram Liaqat, Muhammad Adnan, Abdulaziz Alouffi, Tetsuya Tanaka, Abid Ali

Experimental & applied acarology　94　(1)　18-18　2024年12月26日

DOI： 10.1007/s10493-024-00989-2 　

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Studying teratological abnormalities in ticks are taxonomically important because this poorly understood biological phenomenon causes difficulties in tick's identification. Globally, reports regarding these abnormalities in ticks, reasons of their causes and their impacts are scarce. According to the available published data, there are no studies regarding teratological abnormalities in ticks from Pakistan. Herein, ticks examined for these abnormalities were museum deposited, collected from buffalos, cattle, camels, goats and sheep in 17 districts of Pakistan during 2017-2023. The abnormalities were observed in 88 out of 3279 (2.68%) ticks, representing eight ixodid species (Rhipicephalus microplus, Rhipicephalus turanicus, Rhipicephalus haemaphysaloides, Haemaphysalis bispinosa, Haemaphysalis montgomeryi, Hyalomma anatolicum, Hyalomma scupense, Hyalomma dromedarii). Among abnormal ticks, 67 abnormalities were characterized as local, 12 as general and nine as a combination of both. Local abnormalities were observed in the scutum, palpal segments, anal aperture, spiracular plates, ventral plates, caudal appendage, festoons and posterior grooves. Also, the constrictions and excavations of body parts were observed. General abnormalities included irregular idiosoma and lateral idiosomal bifurcation. This study, for the first time, highlights the diversity of teratological abnormalities in Pakistani ticks, and are reported in in Hy. anatolicum, Ha. bispinosa and Ha. montgomeryi for the first time.
Investigation of Hyalomma turanicum and Hyalomma asiaticum in Pakistan, with notes on the detection of tickborne Rickettsiales. 国際誌査読有り

Zafar Ullah, Iram Liaqat, Mehran Khan, Abdulaziz Alouffi, Mashal M Almutairi, Dmitry A Apanaskevich, Tetsuya Tanaka, Abid Ali

Frontiers in veterinary science　11　1500930-1500930　2024年12月23日

DOI： 10.3389/fvets.2024.1500930 　

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There is limited information on the occurrence of Hyalomma turanicum and Hyalomma asiaticum ticks, as well as associated Rickettsia, Anaplasma, and Ehrlichia species in Pakistan. Addressing this knowledge gap, the current study aimed at morphomolecular confirmation of these ticks and molecular assessment of associated Rickettsiales bacteria (Rickettsia, Anaplasma, and Ehrlichia spp.) in Balochistan, Pakistan. A total of 314 ticks were collected from 74 of 117 (63.2%) hosts, including 41 of 74 (55.4%) goats and 33 of 74 (44.5%) sheep. Subsequently, a subset of microscopically identified ticks was subjected to DNA extraction and PCR to amplify 16S rDNA and cox1 fragments. Additionally, gltA, ompA, and ompB fragments were targeted for Rickettsia spp. and 16S rDNA fragments for both Anaplasma and Ehrlichia spp. The 16S rDNA and cox1 sequences of Hy. turanicum demonstrated 100% identity with those of the same species previously reported from Pakistan. The 16S rDNA and cox1 sequences of Hy. asiaticum exhibited 99.52 and 100% identities, respectively, with corresponding species reported from China, Kazakhstan, and Turkey. The gltA, ompA, and ompB fragments associated with Hy. turanicum showed 100% identities with Rickettsia aeschlimannii reported from Egypt, Italy, Kazakhstan, Kenya, Pakistan, and Senegal. The 16S rDNA sequences of Anaplasma sp. and Ehrlichia sp. associated with both Hy. asiaticum and Hy. turanicum exhibited 99.67 and 100% identities with unknown Anaplasma sp. and Ehrlichia sp. reported from Morocco and Pakistan, respectively. In the 16S rDNA and cox1 phylogenetic trees of ticks, Hy. turanicum and Hy. asiaticum from the current study clustered with their respective species. Similarly, in gltA, ompA, and ompB phylogenetic trees of Rickettsia, R. aeschlimannii of the present study clustered with the same species, whereas Anaplasma sp. and Ehrlichia sp. of this study clustered with undetermined Anaplasma spp. and Ehrlichia spp. in the 16S rDNA phylogenetic tree of Anaplasmataceae. Among the DNA samples from the screened ticks, a coinfection rate of R. aeschlimannii, Anaplasma sp., and Ehrlichia sp. (2 of 80, 2.5%) was observed in Hy. turanicum, whereas individual infection rates were noted as follows: R. aeschlimannii (8 of 80, 10%), Anaplasma sp. (5 of 80, 6.3%), and Ehrlichia sp. (5 of 80, 6.3%). This study marks the first record of molecular characterization of Hy. turanicum and Hy. asiaticum as well as the detection of associated R. aeschlimannii, Anaplasma sp., and Ehrlichia sp. in Balochistan, Pakistan.
Unveiling Misconceptions among Small-Scale Farmers Regarding Ticks and Tick-Borne Diseases in Balochistan, Pakistan. 国際誌査読有り

Zafar Ullah, Mehran Khan, Iram Liaqat, Kashif Kamran, Abdulaziz Alouffi, Mashal M Almutairi, Tetsuya Tanaka, Abid Ali

Veterinary sciences　11　(10)　2024年10月12日

DOI： 10.3390/vetsci11100497 　

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Ticks and tick-borne diseases (TBDs) pose potential health threats to small-scale farmers of grazing animals in the upper highlands of Balochistan, Pakistan. This study was conducted based on a questionnaire survey involving 153 farmers of grazing animals in seven districts to access their knowledge, attitudes, and practices regarding ticks and TBDs. Odds ratios and 95% confidence intervals, based on Fisher's test, were used to assess risk factors for determining preventive measures. The findings revealed a low level of knowledge among the participants. For instance, there was a lack of awareness of the effects of climate change and the economic impact of ticks on animal health. The essential precautions, such as the non-indiscriminate use of acaricides, wearing dark-colored clothing, and limiting children's interaction with grazing animals, were often overlooked. However, the farmers had a positive attitude towards tick control, but they mostly relied on the knowledge of local communities. The neglect of such measures places these farmers and their children at risk of contracting TBDs. This study also indicates minimal involvement from the government in educating farmers and controlling ticks. The role of stakeholders, including the government, non-governmental organizations, veterinary doctors, and local farmer communities, is crucial to address these issues and to implement effective training programs that address misconceptions about ticks and TBDs. Overall, this study highlights the importance of implementing awareness and education programs to address the misconceptions about ticks and TBDs among farmers.
Molecular detection of Rickettsia aeschlimannii, Candidatus Rickettsia shennongii, Rickettsia sp. and Coxiella burnetii in ticks collected from camels. 国際誌査読有り

Shafi Ullah, Abdulaziz Alouffi, Majid Khan, Momin Khan, Hadia Tila, Tetsuya Tanaka, Itabajara da Silva Vaz Jr, Mashal M Almutairi, Abid Ali

Scientific reports　14　(1)　22129-22129　2024年9月27日

DOI： 10.1038/s41598-024-73663-7 　

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Tick-borne bacteria of the genera Rickettsia and Coxiella cause several emerging veterinary and human infectious diseases. Ticks of the genus Hyalomma are medically important vectors due to their potential role in the transmission of pathogens to vertebrate hosts. There is an inadequate knowledge on tick-borne Rickettsia spp. and Coxiella spp. in ticks infesting transhumant camels in Pakistan. In this study, we conducted a molecular survey for screening of Rickettsia spp. and Coxiella spp. in ticks infesting camels. Seven hard tick species including Hyalomma dromedarii, Hyalomma anatolicum, Hyalomma scupense, Hyalomma isaaci, Hyalomma turanicum, Hyalomma asiaticum, and Rhipicephalus sanguineus s.l were confirmed on camels in three distinct physiographic regions of Khyber Pakhtunkhwa, Pakistan. A subset of morphologically identified ticks were subjected to molecular assays for the genetic characterization of ticks and the detection and genetic characterization of Rickettsia and Coxiella species using standard genetic markers. Ticks screened for pathogens resulted in the detection of Rickettsia aeschlimannii and Candidatus Rickettsia shennongii and Coxiella burnetii. The molecular analysis further reveals the presences of an undetermined Rickettsia aeschlimannii-like species, that is making a distinct phylogenetic clade with R. aeschlimannii. The detection of pathogens in camel ticks poses potential health hazards as these ticks frequently bites humans. Molecular screening of Rickettsia spp. and Coxiella spp. associated with camel ticks is a preliminary step toward the surveillance of evaluating their zoonotic threats in the region.
Babesia bigemina and Theileria annulata infections in cattle: molecular detection, phylogenetic analysis, and assessment of risk factors. 国際誌査読有り

Majid Khan, Mehran Khan, Momin Khan, Sidra Batool, Tetsuya Tanaka, Abdulaziz Aloufi, Mashal M Almutairi, Abid Ali

Tropical animal health and production　56　(8)　282-282　2024年9月26日

DOI： 10.1007/s11250-024-04122-8 　

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Babesia bigemina and Theileria annulata are tick-borne protozoans that cause piroplasmosis in cattle, resulting in huge damages to the livestock industry. The prevalence of these infections depends on various intrinsic and extrinsic risk factors. In Pakistan, there is no information regarding the molecular characterization of Babesia bigemina and the risk factors associated with piroplasmosis. This study aimed to molecularly characterize Babesia spp. and Theileria spp. infecting various cattle breeds in Khyber Pakhtunkhwa, Pakistan, and to shed light on risk factors associated with these infections. Altogether, 219 blood samples were collected from various symptomatic cattle breeds, including Holstein Friesian (65.3%; 143/219), Jersey (21.5%; 47/219) and Sahiwal (13.2%; 29/219). Isolated genomic DNA from these blood samples was used in PCR for the amplification of the 18S rRNA fragment of apicomplexan parasites. Obtained 18S rDNA sequences from cattle hosts showed 99.5% identity with B. bigemina, or 100% with T. annulata. Having an overall infection rate of 61.6% (135/219), the highest infection rate was recorded for T. annulata (43.8%; 95/219), followed by B. bigemina (18.3%; 40/219). Phylogenetic analysis of 18S rDNA sequences revealed that B. bigemina clustered with corresponding species reported from Bolivia, and South Africa, while T. annulata grouped with same species from Italy, India, and Turkey. Among the different risk factors, the breed, season, and tick infestation were found to have a significant (P < 0.05) association with the piroplasmid infections. The information obtained in this study can be employed for effective surveillance and control of babesiosis and theileriosis in Pakistan. In addition to confirming our previous molecular detection of T. annulata in cattle, this study provides the first molecular surveillance and phylogenetic position of B. bigemina and associated risk factors in the study region.
Molecular detection of Anaplasma bovis, Candidatus Anaplasma boleense and Rickettsia spp. in ticks infesting small ruminants. 国際誌査読有り

Zaibullah Khan, Farman Ullah, Shafi Ullah, Mohammed Ibrahim, Momin Khan, Gauhar Rehman, Tetsuya Tanaka, Mashal M Almutairi, Abdulaziz Alouffi, Abid Ali

BMC veterinary research　20　(1)　408-408　2024年9月11日

DOI： 10.1186/s12917-024-04259-6 　

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Anaplasma spp. and Rickettsia spp. are intracellular vector-borne pathogens and harbored by a wide range of ticks and vertebrate hosts. Aim of this study was to molecularly characterize Anaplasma spp. and Rickettsia spp. in different ticks collected from livestock hosts in nine districts of Khyber Pakhtunkhwa (KP), Pakistan. In total, 862 ticks were collected from cattle, goats and sheep. Highest tick's infestation was observed on cattle 56.14% (32/57), followed by goats 45.45% (40/88), and sheep 42.05% (45/107). Rhipicephalus microplus (305/862, 35.38%) was predominant species, followed by Haemaphysalis sulcata (243/862, 28.19%), Hyalomma anatolicum (133/862, 15.42%), Haemaphysalis bispinosa (120/862, 13.92%), and Hyalomma kumari (61/862, 7.07%). A subset of 135 ticks were screened for Anaplasma spp. and Rickettsia spp. based on the amplification of partial 16 S rDNA and outer-membrane protein A (ompA) fragments, respectively. In total, 16 ticks (11.85%) were positive for Anaplasma spp. and Rickettsia spp. Obtained 16 S rDNA sequences for Anaplasma spp. detected in Ha. bispinosa and Ha. sulcata showed 99.98% identity with Anaplasma bovis, while other detected in Rh. microplus showed 99.84% identity with Candidatus Anaplasma boleense. Similarly, detected ompA sequence in Ha. sulcata showed 100% identity with Rickettsia sp. and 97.93% with Rickettsia slovaca, and another sequence detected in Rh. microplus showed 100% identity with Candidatus Rickettsia shennongii. In phylogenetic trees, these sequences clustered with corresponding species from Pakistan, China, Turkey, South Korea, South Africa, and Herzegovina. This is the first study reporting detection of A. bovis in Ha. bispinosa and Ha. sulcata, Ca. A. boleense in Rh. microplus collected from goats, and R. slovaca-like in Ha. sulcata. Our results enforce the need for regular surveillance of Rickettsiales in hard ticks infesting livestock in the region.
Targeting Yezo Virus Structural Proteins for Multi-Epitope Vaccine Design Using Immunoinformatics Approach. 国際誌査読有り

Sudais Rahman, Chien-Chun Chiou, Mashal M Almutairi, Amar Ajmal, Sidra Batool, Bushra Javed, Tetsuya Tanaka, Chien-Chin Chen, Abdulaziz Alouffi, Abid Ali

Viruses　16　(9)　2024年9月3日

DOI： 10.3390/v16091408 　

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A novel tick-borne orthonairovirus called the Yezo virus (YEZV), primarily transmitted by the Ixodes persulcatus tick, has been recently discovered and poses significant threats to human health. The YEZV is considered endemic in Japan and China. Clinical symptoms associated with this virus include thrombocytopenia, fatigue, headache, leukopenia, fever, depression, and neurological complications ranging from mild febrile illness to severe outcomes like meningitis and encephalitis. At present, there is no treatment or vaccine readily accessible for this pathogenic virus. Therefore, this research employed an immunoinformatics approach to pinpoint potential vaccine targets within the YEZV through an extensive examination of its structural proteins. Three structural proteins were chosen using specific criteria to pinpoint T-cell and B-cell epitopes, which were subsequently validated through interferon-gamma induction. Six overlapping epitopes for cytotoxic T-lymphocytes (CTL), helper T-lymphocytes (HTL), and linear B-lymphocytes (LBL) were selected to construct a multi-epitope vaccine, achieving a 92.29% coverage of the global population. These epitopes were then fused with the 50S ribosomal protein L7/L12 adjuvant to improve protection against international strains. The three-dimensional structure of the designed vaccine construct underwent an extensive evaluation through structural analysis. Following molecular docking studies, the YEZV vaccine construct emerged as a candidate for further investigation, showing the lowest binding energy (-78.7 kcal/mol) along with favorable physiochemical and immunological properties. Immune simulation and molecular dynamics studies demonstrated its stability and potential to induce a strong immune response within the host cells. This comprehensive analysis indicates that the designed vaccine construct could offer protection against the YEZV. It is crucial to conduct additional in vitro and in vivo experiments to verify its safety and effectiveness.
Molecular detection and characterization of Anaplasma marginale and Babesia canis vogeli infecting dogs in Luxor, Egypt. 国際誌査読有り

Hassan Y A H Mahmoud, Moshera S Shahat, Ragab M Fereig, Alsagher O Ali, Walaa F A Emeish, Ahmed M Soliman, Fatma A Khalifa, Tetsuya Tanaka

Scientific reports　14　(1)　16888-16888　2024年7月23日

DOI： 10.1038/s41598-024-67009-6 　

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Tick-borne diseases in animals are increasing rapidly worldwide, but there is insufficient information about tick-borne diseases infecting dogs in southern Egypt. Thus, in the current study, we detected the presence of Anaplasma marginale (A. marginale) and Babesia canis vogeli (B. canis vogeli) in the blood of dogs. The results revealed that 4/100 (4%) were positive, and a higher infection rate was found in males (75%), than females (25%). The phylogenetic analysis for the major surface protein 4 (msp4) gene in this study was compared with amplicons separate from other reported isolates with alignment by identity 100% with cattle and camels from Egypt, and the phylogenetic analysis for the B. canis vogeli small subunit ribosomal RNA (SSU rRNA) gene in this study identified identity by 99.89% with dogs from Egypt. This report is considered the first report in southern Egypt about A. marginale in dogs based on the sequence analysis of the msp4 gene, providing new data for the classification and identification of A. marginale in dogs compared to A. marginale isolated from other animals in southern Egypt.
Molecular detection and characterization of Anaplasma ovis, Theileria ovis, and Theileria lestoquardi in sheep and goats in Luxor, Egypt. 国際誌査読有り

Hassan Y A H Mahmoud, Tetsuya Tanaka, Alsagher O Ali, Walaa F A Emeish

BMC veterinary research　20　(1)　260-260　2024年6月17日

DOI： 10.1186/s12917-024-04109-5 　

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BACKGROUND: Tick-borne diseases cause economically significant losses to animal production globally, and anaplasmosis and theileriosis are associated with the greatest losses. However, the spread of the relevant pathogens in flocks of domesticated animals in southern Egypt is little understood. Accordingly, in this study, we aimed to determine the prevalences of Anaplasma ovis, Theileria ovis, and Theileria lestoquardi in southern Egyptian sheep and goats through blood tests, and to make a molecular characterization of the A. ovis detected in sheep targeting a specific gene. RESULTS: We collected blood samples collected from 300 sheep and goats (n=150 /species) in Luxor Province in southern Egypt, and analyzed them for the presence of A. ovis, T. ovis and T. lestoquardi with screening by conventional and nested PCR targeting the msp4 and msp5, 18S rRNA, and merozoite surface protein genes. For A. ovis 140/300 samples (46.66%) were positive overall, with 90/150 (60%) and 50/150 (33.33%) positive samples in sheep and goats, respectively. Two major surface protein genes of A. ovis, msp4 and msp5, were sequenced using DNA extracted from sheep and goat blood samples, for phylogenetic analysis and genotyping. The msp4 gene sequence revealed no significant genetic diversity, to contrast to data on A. ovis strains from other countries. For T. lestoquardi, 8/150 (5.33%) samples were positive in sheep, but no samples were positive in goats (0%). For T. ovis, 32/150 (21.33%) samples were positive in sheep, but no samples were positive in goats (0%). Sequencing targeting the merozoite surface protein gene for T. lestoquardi and the small subunit ribosomal RNA gene for T. ovis revealed no significant genetic diversity in the study, another contrast to data on A. ovis strains from other countries. CONCLUSION: This study provides valuable data on phylogenetic and molecular classifications of A. ovis, T. ovis and T. lestoquardi found in southern Egyptian sheep and goats. It also represents the first report on detection and molecular characterization of T. lestoquardi in southern Egyptian sheep based on the specific merozoite surface protein gene, thus providing valuable data for molecular characterization of this pathogen in southern Egypt.
Subtractive Proteomics and Reverse-Vaccinology Approaches for Novel Drug Target Identification and Chimeric Vaccine Development against Bartonella henselae Strain Houston-1. 国際誌査読有り

Sudais Rahman, Chien-Chun Chiou, Shabir Ahmad, Zia Ul Islam, Tetsuya Tanaka, Abdulaziz Alouffi, Chien-Chin Chen, Mashal M Almutairi, Abid Ali

Bioengineering (Basel, Switzerland)　11　(5)　2024年5月17日

DOI： 10.3390/bioengineering11050505 　

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Bartonella henselae is a Gram-negative bacterium causing a variety of clinical symptoms, ranging from cat-scratch disease to severe systemic infections, and it is primarily transmitted by infected fleas. Its status as an emerging zoonotic pathogen and its capacity to persist within host erythrocytes and endothelial cells emphasize its clinical significance. Despite progress in understanding its pathogenesis, limited knowledge exists about the virulence factors and regulatory mechanisms specific to the B. henselae strain Houston-1. Exploring these aspects is crucial for targeted therapeutic strategies against this versatile pathogen. Using reverse-vaccinology-based subtractive proteomics, this research aimed to identify the most antigenic proteins for formulating a multi-epitope vaccine against the B. henselae strain Houston-1. One crucial virulent and antigenic protein, the PAS domain-containing sensor histidine kinase protein, was identified. Subsequently, the identification of B-cell and T-cell epitopes for the specified protein was carried out and the evaluated epitopes were checked for their antigenicity, allergenicity, solubility, MHC binding capability, and toxicity. The filtered epitopes were merged using linkers and an adjuvant to create a multi-epitope vaccine construct. The structure was then refined, with 92.3% of amino acids falling within the allowed regions. Docking of the human receptor (TLR4) with the vaccine construct was performed and demonstrated a binding energy of -1047.2 Kcal/mol with more interactions. Molecular dynamic simulations confirmed the stability of this docked complex, emphasizing the conformation and interactions between the molecules. Further experimental validation is necessary to evaluate its effectiveness against B. henselae.
Discovery of Colpodella spp. in ticks (Hyalomma dromedarii) infesting camels in southern Egypt. 国際誌査読有り

Ahmed M Soliman, Hassan Y A H Mahmoud, Tatsuro Hifumi, Tetsuya Tanaka

Ticks and tick-borne diseases　15　(5)　102352-102352　2024年5月16日

DOI： 10.1016/j.ttbdis.2024.102352 　

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In Egypt, tick-borne diseases pose a significant threat to human and animal health, and the threat to dromedaries (Camelus dromedarius), the country's dominant camelid species, is of particular concern. These animals are frequently infested with ticks, and may thus develop tick-borne diseases or become reservoirs of tick-borne pathogens. However, there is a paucity of data on tick infestation in Egyptian camels, especially in the south of the country. Accordingly, we aimed to determine the prevalence of tick infestation in southern Egyptian camel populations (in Luxor and Aswan governorates), and identify the hemoprotozoan parasites carried by camel-infesting ticks. Camels were checked for ticks during veterinary examination at quarantine and household checks, and ticks were collected from infested camels for species identification using morphological examination and PCR analyses. Tick and hemoprotozoan species were identified using Basic Local Alignment Search Tool analysis with subsequent confirmation in phylogenetic analyses. All camel-infesting ticks belonged to the species Hyalomma dromedarii, and were clustered with ticks of this species previously found in Egypt in a phylogenetic tree based on the 16S rRNA gene. Molecular analysis targeting the 18S rRNA gene revealed the presence of hitherto undetected hemoprotozoan parasites, Colpodella spp., in 30/297 (10.1 %) camel-infesting ticks. In phylogenetic analysis, these Colpodella spp. were highly homologous (94-98.6 %) with Colpodella spp. previously deposited in GenBank with accession numbers OQ540590Q, MH208621, and GQ411073, which relate to Colpodella spp. previously detected from Haemaphysalis longicornis, Rhipicephalus haemaphysaloides, and humans in China. PCR analyses with spherical body protein-4 (SBP-4) gene-specific primers revealed Babesia bovis in 16/297 (5 %) of camel-infesting ticks, however, Babesia bigemina and Theileria annulata were not detected. Here, we report the first detection of Colpodella spp. in H. dromedarii in Egypt. Further epidemiological studies are needed to assess the risk to camels and humans, and the transmission dynamics. Based on the high tick infestation rates in Egyptian camels and the identification of previously unreported protozoan hemoparasites in ticks, we consider that the dromedary should be subject to surveillance as a sentinel species for tick-borne diseases in Egypt. Our findings underline the need for surveillance and collecting data on lesser known pathogens circulating in camel-infesting ticks, as part of a public health strategy for dealing with tick-borne diseases in Egypt.
Molecular detection and characterization of Theileria annulata, Babesia bovis, and Babesia bigemina infecting cattle and buffalo in southern Egypt. 国際誌査読有り

Hassan Y A H Mahmoud, Abdelrahman A Rady, Tetsuya Tanaka

Parasite epidemiology and control　25　e00340　2024年5月

DOI： 10.1016/j.parepi.2024.e00340 　

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Tick-borne diseases have a major adverse effect on livestock worldwide, causing enormous economic losses in meat and milk production as well threatening animal and public health. In this study, we aimed to detect and characterize piroplasms isolated from cattle and buffalo in southern Egypt, using molecular techniques. Three hundred blood samples were collected from cattle and buffalo in two governorates in southern Egypt. All 300 samples (100%) were confirmed to contain DNA, as they exhibited bands of bovine β-actin gene at the expected 227 bp for cattle and buffalo. The samples were analyzed by PCR for the presence of piroplasms, specifically Babesia bovis, Babesia bigemina, and Theileria annulata. Samples positive for the piroplasma 18S ribosomal RNA gene were further examined for two additional genes, spherical body protein 4 gene, to provide an enhanced degree of specificity for the identification of B. bovis and B. bigemina, and the major merozoite surface antigen gene for T. annulata. The infection rate for piroplasma spp. was 60/300 (20%). The positivity rates were 10.7% (32/300) for T. annulata, 5.3% (16/300) for B. bovis, and 4% (12/300) for B. bigemina. By host species, 42/150 (28%) cattle and 18/150 (12%) buffalo were positive for piroplasms. None of the isolates sequenced for the B. bovis isolates from buffalo in this study showed 100% identity with any sequence deposited in GenBank for the small subunit ribosomal RNA gene (maximum identity value = 99.74%). Similarly, no T. annulata small subunit ribosomal RNA gene sequence identified in this study exhibited 100% identity with any sequence deposited in GenBank (maximum identity value = 99.89%). The current study provides a partial sequence of the T. annulata merozoite-piroplasm surface antigen gene, as well as the B. bovis and B. bigemina spherical body protein 4 genes, in cattle and buffalo in southern Egypt, and is the first report on these piroplasma genes in cattle and buffalo in southern Egypt.
Molecular detection and diversity of tick-borne rickettsial pathogens in ticks collected from camel (Camelus dromedarius) in Upper Egypt. 国際誌査読有り

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Tatsuro Hifumi, Tetsuya Tanaka

Acta tropica　253　107172-107172　2024年3月5日

DOI： 10.1016/j.actatropica.2024.107172 　

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Tick-borne rickettsial pathogens pose significant threats to public and animal health. In Upper Egypt, limited information exists regarding the prevalence and diversity of such tick-borne pathogens. Therefore, this study aimed to conduct a comprehensive investigation to elucidate the presence and variety of tick-borne rickettsial pathogens in Upper Egyptian camels. Our results revealed a prevalence of 2.96 % for Anaplasma marginale and 0.34 % for Candidatus Anaplasma camelii among Hyalomma ticks. However, Ehrlichia spp. weren't detected in our study. The identification of Ca. A. camelii in H. dromedari ticks was documented for the first time, suggesting a potential mode of transmission in camels. Notably, this study marks the first documentation of Rickettsia aeschlimannii with a prevalence of 6.06 % in the study area. Furthermore, we detected Coxiella burnetii in a prevalence of 8.08 % in Hyalomma ticks, indicating a potential risk of Q fever transmission. Molecular techniques results were confirmed by sequencing and phylogenetic analysis and provided valuable insights into the epidemiology of these pathogens, revealing their diversity. This study is vital in understanding tick-borne rickettsial pathogens' prevalence, distribution, and transmission dynamics in Upper Egypt. In conclusion, our findings emphasize the importance of continued research to enhance our understanding of the epidemiology and impact of these pathogens on both animal and human populations.
Molecular phylogenetic analysis of Echinococcus multilocularis from horses raised in Canada or Japan, using mitochondrial cytochrome b gene-targeted PCR. 国際誌査読有り

Tatsuro Hifumi, Tetsuya Tanaka, Ichinosuke Suzu, Miho Sato, Kohei Akioka, Chiaki Fujimata, Ryohei Shinkai, Tsutomu Maeda, Kodai Kusakisako, Hiromi Ikadai, Noriaki Miyoshi

Food and waterborne parasitology　34　e00219　2024年3月

DOI： 10.1016/j.fawpar.2024.e00219 　

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Alveolar echinococcosis is a zoonotic disease caused by a larval-stage Echinococcus multilocularis infection. Geographical haplotyping targeting the parasite's mitochondrial cytochrome b (cob) gene has been reported for isolates from definitive and intermediate hosts (wild canids and rodents); however, there are limited reports on strain typing for the dead-end host, the horse, which could act as a sentinel for E. multilocularis. Accordingly, we investigated the diversity of E. multilocularis in isolates obtained from slaughtered Japanese and Canadian horses originating from the Iburi and Hidaka regions in Hokkaido and from Alberta, respectively, with PCR and haplogroup analyses targeting cob gene sequences obtained. Seventy horses were diagnosed with alveolar echinococcosis based on histopathology and cob-gene PCR testing. The E. multilocularis detected in these horses was classified as either an Asian (for Hokkaido-raised horses) or a European (for Alberta-raised horses) haplogroup, based on the obtained cob-gene sequence analysis. In addition, haplotype network analysis revealed that E. multilocularis isolated from Hokkaido-raised horses is highly homologous to Kazakhstan isolates, and E. multilocularis isolated from Alberta-raised horses is highly homologous to Austrian isolates. The results of this study suggest that cob-gene-targeted PCR analysis could be useful for the geographical genetic characterization of E. multilocularis isolated from horses.
First report of spotted fever group Rickettsia aeschlimannii in Hyalomma turanicum, Haemaphysalis bispinosa, and Haemaphysalis montgomeryi infesting domestic animals: updates on the epidemiology of tick-borne Rickettsia aeschlimannii. 国際誌査読有り

Abdul Majid, Mashal M Almutairi, Abdulaziz Alouffi, Tetsuya Tanaka, Tsai-Ying Yen, Kun-Hsien Tsai, Abid Ali

Frontiers in microbiology　14　1283814-1283814　2023年12月

DOI： 10.3389/fmicb.2023.1283814 　

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Tick-borne Rickettsia spp. have long been known as causative agents for zoonotic diseases. We have previously characterized Rickettsia spp. in different ticks infesting a broad range of hosts in Pakistan; however, knowledge regarding Rickettsia aeschlimannii in Haemaphysalis and Hyalomma ticks is missing. This study aimed to obtain a better understanding about R. aeschlimannii in Pakistan and update the knowledge about its worldwide epidemiology. Among 369 examined domestic animals, 247 (66%) were infested by 872 ticks. Collected ticks were morphologically delineated into three genera, namely, Rhipicephalus, Hyalomma, and Haemaphysalis. Adult females were the most prevalent (number ₌ 376, 43.1%), followed by nymphs (303, 34.74%) and males (193, 22.13%). Overall, genomic DNA samples of 223 tick were isolated and screened for Rickettsia spp. by the amplification of rickettsial gltA, ompA, and ompB partial genes using conventional PCR. Rickettsial DNA was detected in 8 of 223 (3.58%) ticks including nymphs (5 of 122, 4.0%) and adult females (3 of 86, 3.48%). The rickettsial gltA, ompA, and ompB sequences were detected in Hyalomma turanicum (2 nymphs and 1 adult female), Haemaphysalis bispinosa (1 nymph and 1 adult female), and Haemaphysalis montgomeryi (2 nymphs and 1 adult female). These rickettsial sequences showed 99.71-100% identity with R. aeschlimannii and phylogenetically clustered with the same species. None of the tested Rhipicephalus microplus, Hyalomma isaaci, Hyalomma scupense, Rhipicephalus turanicus, Hyalomma anatolicum, Rhipicephalus haemaphysaloides, Rhipicephalus sanguineus, Haemaphysalis cornupunctata, and Haemaphysalis sulcata ticks were found positive for rickettsial DNA. Comprehensive surveillance studies should be adopted to update the knowledge regarding tick-borne zoonotic Rickettsia species, evaluate their risks to humans and livestock, and investigate the unexamined cases of illness after tick bite among livestock holders in the country.
Isolation, identification and antimicrobial susceptibility of the bacteria isolated from Hyalomma dromedarii infesting camels in Al-Jouf province, Saudi Arabia. 国際誌査読有り

Alanoud T Aljasham, Eman M Damra, Nora S Alkahtani, Abdulaziz Alouffi, Waleed S Al Salem, Aljoharah O Alshabanah, Moureq Alotaibi, Tetsuya Tanaka, Abid Ali, Mashal M Almutairi

Frontiers in veterinary science　10　1227908-1227908　2023年12月

DOI： 10.3389/fvets.2023.1227908 　

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Ticks are important ectoparasites that transmit various pathogens causing morbidity and mortality in humans and animals. Saudi Arabia faces several challenges that can contribute to the emergence and spread of antimicrobial resistance (AMR) bacteria. These challenges require collaborative efforts to successfully achieve significant control of AMR in the country. The present study aims to isolate bacteria from camels' tick Hyalomma dromedarii in Al-Jouf province to identify and determine these isolates' antimicrobial susceptibilities. Forty-nine ticks were collected from dromedary camels and morphologically classified as H. dromedarii. Ticks were then homogenized and plated individually, which resulted in the isolation of 55 bacteria. The results showed that the bacterial isolates belong to 20 different species. About 71% (n = 39) of the total isolates were identified as Gram-positive bacteria comprised of 11 different species, while 29% (n = 16) of the total isolates were Gram-negative bacteria comprised of 9 different species. The most prevalent isolate within the total samples was Staphylococcus lentus (22.45%, 11/49), followed by Staphylococcus pseudintermedius (18.37%, 9/49) and Sphingomonas paucimobilis (16.33% 8/49). The antimicrobial susceptibility profile of Gram-positive bacteria showed that 100% (n = 31) were resistant to benzylpenicillin; 90.3% (n = 28) were resistant to oxacillin; 58.1% (n = 18) were resistant to clindamycin; 48.4% (n = 15) were resistant to vancomycin. In addition, 32.3% (n = 10) were resistant to trimethoprim/sulfamethoxazole and rifampicin; 25.8% (n = 8) were resistant to erythromycin; 16.1% (n = 5) were resistant to teicoplanin; 6.5% (n = 2) were resistant to tetracycline. All Gram-positive bacteria were 100% susceptible to linezolid, gentamicin, tobramycin, levofloxacin, moxifloxacin, tigecycline, and nitrofurantoin. In antimicrobial susceptibility tests for the Gram-negative bacteria, 57.14% (n = 8) of the identified bacteria were resistant to ampicillin, whereas 50% (n = 7) were resistant to cefoxitin and ceftazidime. About 28.57% (n = 4) of the Gram-negative bacteria were resistant to ceftriaxone, trimethoprim/sulfamethoxazole. In addition, 21.43% (n = 3) were resistant to amoxicillin/clavulanic acid and cephalothin; 14.29% (n = 2) were resistant to cefepime and nitrofurantoin; 7.14% (n = 1) were resistant to piperacillin/tazobactam and tigecycline. However, all Gram-negative bacteria were susceptible to other examined antimicrobials. This is the first study that investigates the role of the hard tick as a potential reservoir for AMR pathogens within our region.
Molecular evidence of Borrelia theileri and closely related Borrelia spp. in hard ticks infesting domestic animals. 国際誌査読有り

Mehran Khan, Mashal M Almutairi, Abdulaziz Alouffi, Tetsuya Tanaka, Shun-Chung Chang, Chien-Chin Chen, Abid Ali

Frontiers in veterinary science　10　1297928-1297928　2023年12月

DOI： 10.3389/fvets.2023.1297928 　

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Ticks pose significant threats to hosts by transmitting Borrelia spp., which are grouped into Lyme borreliae, relapsing fever borreliae (RF), and reptiles- and monotremes-associated borreliae. The RF borreliae encompass a group of Borrelia species predominantly transmitted by soft ticks, but some of its members can also be transmitted by hard ticks. Information on the detection and genetic characterization of tick-borne RF borreliae, including Borrelia theileri, is notably rare in Asia, particularly in Pakistan. Herein, we employed molecular techniques to detect borreliae in hard ticks collected from domestic animals in Khyber Pakhtunkhwa, Pakistan. Ticks were subjected to morphological analysis, followed by DNA extraction and PCR amplification of partial fragments of borrelial 16S rRNA and flaB genes. A total of 729 ticks were collected from 264 hosts, with Haemaphysalis cornupunctata (12.9%; 94/729) being the most prevalent, followed by Hyalomma anatolicum (11.7%; 85/729), Rhipicephalus microplus (10.0%; 73/729), Haemaphysalis kashmirensis (9.1%; 66/729), Haemaphysalis bispinosa (8.5%; 62/729), Rhipicephalus sanguineus (8%; 58/729), Haemaphysalis montgomeryi (6.2%; 45/729), Rhipicephalus turanicus (5.5%; 40/729), Hyalomma dromedarii and Ixodes kashmirensis (4.4%; 32/729 each), Rhipicephalus haemaphysaloides (4.1%; 30/729), Haemaphysalis sulcata and Hyalomma scupense (3.8%; 28/729 each), Haemaphysalis danieli (2.9%; 21/729), Hyalomma kumari (2.6%; 19/729), and Hyalomma isaaci (2.2%; 16/729). Based on 16S rRNA detection of Borrelia spp., only R. turanicus yielded positive results, resulting in an overall infection rate of 0.3% (2/160), while using flaB-based detection, four tick species including R. microplus, R. turanicus, Ha. sulcata, and Ha. cornupunctata showed positive results, yielding an overall infection rate of 6.9% (11/160). The amplified DNA fragments of borrelial 16S rRNA and flaB in R. turanicus from goats shared maximum identities of 100 and 99.40% with Borrelia theileri, respectively. Amplified borrelial flaB fragments in R. microplus from cows and sheep displayed 100% identity with B. theileri, while flaB fragments in Ha. cornupunctata and Ha. sulcata from goats revealed identities of 99.32 and 99.75% with undetermined RF Borrelia spp., respectively. Phylogenetic analysis revealed clustering of B. theileri from R. microplus and R. turanicus with the same species, while Borrelia spp. from Ha. cornupunctata and Ha. sulcata with undetermined RF Borrelia spp. Notably, this research marks the first documentation of B. theileri in R. turanicus and the identification of RF Borrelia spp. in Ha. cornupunctata and Ha. sulcata.
First Molecular-Based Confirmation of Dermacentor marginatus and Associated Rickettsia raoultii and Anaplasma marginale in the Hindu Kush Mountain Range. 国際誌査読有り

Iftikhar Ahmad, Shafi Ullah, Abdulaziz Alouffi, Mashal M Almutairi, Muhammad Numan, Tetsuya Tanaka, Shun-Chung Chang, Chien-Chin Chen, Abid Ali

Animals : an open access journal from MDPI　13　(23)　2023年11月28日

DOI： 10.3390/ani13233686 　

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Ticks of the genus Dermacentor Koch, 1844 (Acari: Ixodidae) are poorly known systematically due to their habitation in harsh topographic environments and high mountains. Dermacentor ticks are diversely distributed in the Palearctic, Nearctic, and Oriental regions. There is no available information on the occurrence of Dermacentor marginatus in Pakistan; thus, the current investigation aimed the first morphological and molecular confirmation of this species and associated Anaplasma marginale and Rickettsia raoultii. Ticks were collected from goats (Capra hircus) and morphologically identified. Genomic DNA was extracted from 18/26 (69.23%) tick specimens, including 11 males and 7 females (1 unfed and 6 fed females). Extracted DNA was subjected to PCR for the amplification of genetic markers like 16S rDNA and cox1 for ticks, 16S rDNA for Anaplasma spp., and gltA and ompB for Rickettsia spp. A total of 26 D. marginatus ticks composed of 19 males (73.07%) and 7 females (26.9%) [1 (3.84%) unfed and 6 (23.07%) fed females] were collected from goats. According to amplicons via BLAST analysis, the 16S rDNA sequence showed 97.28-98.85% identity and the cox1 sequence showed 95.82-98.03% identity with D. marginatus. Additionally, the 16S rDNA sequence for Anaplasma sp. was detected in D. marginatus that showed 100% identity with Anaplasma marginale. Rickettsial gltA and ompB sequences for Rickettsia sp. showed 100% identity with Rickettsia raoultii. In phylogenetic analysis, ticks' 16S rDNA and cox1 sequences clustered with the same species. In phylogenetic analysis, A. marginale based on 16 rDNA clustered with A. marginale, while gltA and ompB sequences clustered with R. raoultii. This is the first study on the genetic characterization of D. marginatus and associated A. marginale and R. raoultii in Pakistan. The northern areas of Pakistan, which need to be explored in terms of ticks and associated pathogens due to their zoonotic threats, have been neglected due to the inaccessible climatic conditions.
Molecular Survey of Rickettsia raoultii in Ticks Infesting Livestock from Pakistan with Notes on Pathogen Distribution in Palearctic and Oriental Regions. 国際誌査読有り

Shehla Shehla, Mashal M Almutairi, Abdulaziz Alouffi, Tetsuya Tanaka, Shun-Chung Chang, Chien-Chin Chen, Abid Ali

Veterinary sciences　10　(11)　2023年10月29日

DOI： 10.3390/vetsci10110636 　

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Ticks are hematophagous ectoparasites that transmit different pathogens such as Rickettsia spp. to domestic and wild animals as well as humans. Genetic characterizations of Rickettsia spp. from different regions of Pakistan are mostly based on one or two genetic markers and are confined to small sampling areas and limited host ranges. Therefore, this study aimed to molecularly screen and genetically characterize Rickettsia spp. in various tick species infesting camels, sheep, and goats. All the collected tick specimens were morphologically identified, and randomly selected tick species (148) were screened molecularly for the detection of Rickettsia spp. by amplifying three rickettsial DNA fragments, namely, the citrate-synthase gene (gltA), outer-membrane protein A (ompA), and outer-membrane protein B (ompB). After examining 261 hosts, 161 (61.7%) hosts were found infested by 564 ticks, including 287 (50.9%) nymphs, 171 (30.3%) females, and 106 (18.8%) males in five districts (Kohat, Dera Ismail Khan, Lower Dir, Bajaur, and Mansehra). The highest occurrence was noted for Hyalomma dromedarii (number = 72, 12.8%), followed by Haemaphysalis sulcata (n = 70, 12.4%), Rhipicephalus turanicus (n = 64, 11.3%), Rhipicephalus microplus (n = 55, 9.7%), Haemaphysalis cornupunctata (n = 49, 8.7%), Hyalomma turanicum (n = 48, 8.5%), Hyalomma isaaci (n = 45, 8.0%), Haemaphysalis montgomeryi (n = 44, 7.8%), Hyalomma anatolicum (n = 42, 7.5%), Haemaphysalis bispinosa (n = 38, 6.7%), and Rhipicephalus haemaphysaloides (n = 37, 6.6%). A subset of 148 ticks were tested, in which eight (5.4%) ticks, including four Hy. turanicum, two Ha. cornupunctata, one Ha. montgomeryi, and one Ha. bispinosa, were found positive for Rickettsia sp. The gltA, ompA, and ompB sequences revealed 100% identity and were phylogenetically clustered with Rickettsia raoultii reported in China, Russia, USA, Turkey, Denmark, Austria, Italy, and France. Additionally, various reports on R. raoultii from Palearctic and Oriental regions were summarized in this study. To the best of our knowledge, this is the first report regarding genetic characterization and phylogenetic analysis of R. raoultii from Pakistan. Further studies to investigate the association between Rickettsia spp. and ticks should be encouraged to apprise effective management of zoonotic consequences.
Argas persicus and Carios vespertilionis Ticks Infesting Ducks, Domestic Fowls and Bats in Pakistan: First Report on Molecular Survey and Phylogenetic Position of Borrelia anserina. 国際誌査読有り

Hafsa Zahid, Abdulaziz Alouffi, Mashal M Almutairi, Muhammad Ateeq, Tetsuya Tanaka, Shun-Chung Chang, Chien-Chin Chen, Abid Ali

Veterinary sciences　10　(10)　2023年10月20日

DOI： 10.3390/vetsci10100628 　

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Argasid ticks have the vectorial potential for transmitting disease-causing pathogens to avian hosts, resulting in economic losses that may not be fully estimated. Borrelia species are the responsible agents of borreliosis in poultry, animals and humans. Our previous studies have reported a high prevalence of Argas persicus infesting domestic fowls in Khyber Pakhtunkhwa (KP), Pakistan. However, molecular screening and genetic characterization of Borrelia spp. in A. persicus have been neglected in Pakistan. In this study, we focused on the molecular epidemiology and genetic characterization of Borrelia spp. associated with A. persicus ticks infesting domestic fowls and ducks, and Carios vespertilionis infesting bats in selected districts of KP. Overall, 1818 ticks, including females (415; 23%), males (345; 19%), nymphs (475; 26%) and larvae (583; 32%), were collected from 27 locations in nine districts (Peshawar, Mardan, Swabi, Charsadda, Chitral, Lakki Marwat, Bannu, Bajaur and Hangu) from domestic fowls, ducks and their shelters, and bats. A subset of 197 ticks was selected for DNA extraction and PCR to amplify fragments of the cytochrome c oxidase (cox) gene for ticks and flagellin B (flaB) for the detection and genetic characterization of associated Borrelia spp. Among these, only Borrelia anserina DNA was detected in 40 ticks (27.2%) of different life stages, where highest prevalence was found in female ticks (18; 45%), followed by nymphs (12; 30%), larvae (7; 17.5%) and males (3; 7.5%). Tick infestation in shelters (1081; 77%) was higher than on hosts (323; 23%). The resultant cox amplicons of A. persicus showed 100% identity with the same species reported from Pakistan, China, Iran, Kenya, Kazakhstan, Algeria and Egypt and C. vespertilionis show 100% identity with the species reported from Pakistan, China, Japan, Kenya, Vietnam, Spain, Netherlands, the United Kingdom and Hungry, and clustered with the aforementioned species in the phylogenetic tree. The obtained Borrelia sequences showed 100% identity with B. anserina and revealed a close resemblance to the relapsing fever group and clustered in a monophyletic clade with B. anserina from India, Iran and Brazil in a phylogenetic tree. These results establish the first molecular characterization of B. anserina in A. persicus infesting domestic fowls and ducks in the region, as well as their shelters. To effectively control zoonotic consequences, country-wide surveillance research should be encouraged to screen soft ticks infesting various birds for associated pathogens.
First Detection of Theileria sinensis-like and Anaplasma capra in Ixodes kashmiricus: With Notes on cox1-Based Phylogenetic Position and New Locality Records. 国際誌査読有り

Muhammad Numan, Abdulaziz Alouffi, Mashal M Almutairi, Tetsuya Tanaka, Haroon Ahmed, Haroon Akbar, Muhammad Imran Rashid, Kun-Hsien Tsai, Abid Ali

Animals : an open access journal from MDPI　13　(20)　2023年10月17日

DOI： 10.3390/ani13203232 　

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Ixodes ticks transmit Theileria and Anaplasma species to a wide range of animals. The spreading of ticks and tick-borne pathogens has been attributed to transhumant herds, and research on these uninvestigated issues has been neglected in many countries, including Pakistan. Recently, we used internal transcribed spacer (ITS) and 16S ribosomal DNA partial sequences to genetically characterize Ixodes kashmiricus ticks and their associated Rickettsia spp. However, the data on its cox1 sequence and associated Theileria spp. and Anaplasma spp. are missing. This study aimed to genetically characterize I. kashmiricus based on the cox1 sequence and their associated Theileria spp. and Anaplasma spp. The I. kashmiricus ticks were collected from small ruminants: sheep (Ovis aries) and goats (Capra hircus) of transhumant herds in district Shangla, Dir Upper and Chitral, Khyber Pakhtunkhwa (KP), Pakistan. Out of 129 examined hosts, 94 (72.87%) (56 sheep and 38 goats) were infested by 352 ticks, including adult females (175; 49.7%) followed by nymphs (115; 32.7%) and males (62; 17.6%). For molecular analyses, 121 ticks were subjected to DNA isolation and PCR for the amplification of the cox1 sequence for I. kashmiricus, 18S rDNA for Theileria spp. and 16S rDNA sequences for Anaplasma spp. The obtained cox1 sequence showed 89.29%, 88.78%, and 88.71% identity with Ixodes scapularis, Ixodes gibbosus, and Ixodes apronophorus, respectively. Phylogenetically, the present cox1 sequence clustered with the Ixodes ricinus complex. Additionally, the 18S rDNA sequence showed 98.11% maximum identity with Theileria cf. sinensis and 97.99% identity with Theileria sinensis. Phylogenetically, Theileria spp. clustered with the T. cf. sinensis and T. sinensis. In the case of Anaplasma spp., the 16S rDNA sequence showed 100% identity with Anaplasma capra and phylogenetically clustered with the A. capra. PCR-based DNA detection targeting the amplification of groEL and flaB sequences of Coxiella spp. and Borrelia spp., respectively, was unsuccessful. This is the first phylogenetic report based on cox1 and new locality records of I. kashmiricus, and the associated T. sinensis-like and A. capra. Significant tick surveillance studies are needed in order to determine the epidemiology of Ixodes ticks and their associated pathogens.
Molecular Detection of Rickettsia hoogstraalii in Hyalomma anatolicum and Haemaphysalis sulcata: Updated Knowledge on the Epidemiology of Tick-Borne Rickettsia hoogstraalii. 国際誌査読有り

Aneela Aneela, Mashal M Almutairi, Abdulaziz Alouffi, Haroon Ahmed, Tetsuya Tanaka, Itabajara da Silva Vaz Junior, Shun-Chung Chang, Chien-Chin Chen, Abid Ali

Veterinary sciences　10　(10)　2023年10月4日

DOI： 10.3390/vetsci10100605 　

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Ticks are hematophagous ectoparasites that transmit pathogens to animals and humans. Updated knowledge regarding the global epidemiology of tick-borne Rickettsia hoogstraalii is dispersed, and its molecular detection and genetic characterization are missing in Pakistan. The current study objectives were to molecularly detect and genetically characterize Rickettsia species, especially R. hoogstraalii, in hard ticks infesting livestock in Pakistan, and to provide updated knowledge regarding their global epidemiology. Ticks were collected from livestock, including goats, sheep, and cattle, in six districts of Khyber Pakhtunkhwa (KP) Pakistan. Overall, 183 hosts were examined, of which 134 (73.2%), including goats (number = 39/54, 72.2%), sheep (23/40, 57.5%), and cattle (71/89, 80%) were infested by 823 ticks. The most prevalent tick species was Rhipicephalus microplus (number = 283, 34.3%), followed by Hyalomma anatolicum (223, 27.0%), Rhipicephalus turanicus (122, 14.8%), Haemaphysalis sulcata (104, 12.6%), Haemaphysalis montgomeryi (66, 8.0%), and Haemaphysalis bispinosa (25, 3.03%). A subset of 210 ticks was selected and screened for Rickettsia spp. using PCR-based amplification and subsequent sequencing of rickettsial gltA and ompB fragments. The overall occurrence rate of R. hoogstraalii was 4.3% (number = 9/210). The DNA of Rickettsia was detected in Hy. anatolicum (3/35, 8.5%) and Ha. sulcata (6/49, 12.2%). However, no rickettsial DNA was detected in Rh. microplus (35), Rh. turanicus (35), Ha. montgomeryi (42), and Ha. bispinosa (14). The gltA and ompB fragments showed 99-100% identity with R. hoogstraalii and clustered phylogenetically with the corresponding species from Pakistan, Italy, Georgia, and China. R. hoogstraalii was genetically characterized for the first time in Pakistan and Hy. anatolicum globally. Further studies should be encouraged to determine the role of ticks in the maintenance and transmission of R. hoogstraalii in different hosts.
Association of SFG Rickettsia massiliae and Candidatus Rickettsia shennongii with Different Hard Ticks Infesting Livestock Hosts. 国際誌査読有り

Shehla Shehla, Farman Ullah, Abdulaziz Alouffi, Mashal M Almutairi, Zaibullah Khan, Tetsuya Tanaka, Marcelo B Labruna, Kun-Hsien Tsai, Abid Ali

Pathogens (Basel, Switzerland)　12　(9)　2023年8月24日

DOI： 10.3390/pathogens12091080 　

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Ixodid ticks are responsible for the transmission of various intracellular bacteria, such as the Rickettsia species. Little Information is available about the genetic characterization and epidemiology of Rickettsia spp. The current study was designed to assess the tick species infesting various livestock hosts and the associated Rickettsia spp. in Pakistan. Ticks were collected from different livestock hosts (equids, cattle, buffaloes, sheep, goats, and camels); morphologically identified; and screened for the genetic characterization of Rickettsia spp. by the amplification of partial fragments of the gltA, ompA and ompB genes. Altogether, 707 ticks were collected from 373 infested hosts out of 575 observed hosts. The infested hosts comprised 105 cattle, 71 buffaloes, 70 sheep, 60 goats, 34 camels, and 33 equids. The overall occurrence of Rickettsia spp. was 7.6% (25/330) in the tested ticks. Rickettsia DNA was detected in Rhipicephalus haemaphysaloides (9/50, 18.0%), followed by Rhipicephalus turanicus (13/99, 13.1%), Haemaphysalis cornupunctata (1/18, 5.5%), and Rhipicephalus microplus (2/49, 4.1%); however, no rickettsial DNA was detected in Hyalomma anatolicum (71), Hyalomma dromedarii (35), and Haemaphysalis sulcata (8). Two Rickettsia agents were identified based on partial gltA, ompA, and ompB DNA sequences. The Rickettsia species detected in Rh. haemaphysaloides, Rh. turanicus, and Rh. microplus showed 99-100% identity with Rickettsia sp. and Candidatus Rickettsia shennongii, and in the phylogenetic trees clustered with the corresponding Rickettsia spp. The Rickettsia species detected in Rh. haemaphysaloides, Rh. turanicus, Rh. microplus, and Ha. cornupunctata showed 100% identity with R. massiliae, and in the phylogenetic trees it was clustered with the same species. Candidatus R. shennongii was characterized for the first time in Rh. haemaphysaloides, Rh. turanicus, and Rh. microplus. The presence of SFG Rickettsia spp., including the human pathogen R. massiliae, indicates a zoonotic risk in the study region, thus stressing the need for regular surveillance.
RNA activation in ticks. 国際誌査読有り

Kofi Dadzie Kwofie, Emmanuel Pacia Hernandez, Anisuzzaman, Hayato Kawada, Yuki Koike, Sana Sasaki, Takahiro Inoue, Kei Jimbo, Fusako Mikami, Danielle Ladzekpo, Rika Umemiya-Shirafuji, Kayoko Yamaji, Tetsuya Tanaka, Makoto Matsubayashi, Md Abdul Alim, Samuel Kweku Dadzie, Shiroh Iwanaga, Naotoshi Tsuji, Takeshi Hatta

Scientific reports　13　(1)　9341-9341　2023年6月8日

DOI： 10.1038/s41598-023-36523-4 　

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RNA activation (RNAa) is a burgeoning area of research in which double-stranded RNAs (dsRNAs) or small activating RNAs mediate the upregulation of specific genes by targeting the promoter sequence and/or AU-rich elements in the 3'- untranslated region (3'-UTR) of mRNA molecules. So far, studies on the phenomenon have been limited to mammals, plants, bacteria, Caenorhabditis elegans, and recently, Aedes aegypti. However, it is yet to be applied in other arthropods, including ticks, despite the ubiquitous presence of argonaute 2 protein, which is an indispensable requirement for the formation of RNA-induced transcriptional activation complex to enable a dsRNA-mediated gene activation. In this study, we demonstrated for the first time the possible presence of RNAa phenomenon in the tick vector, Haemaphysalis longicornis (Asian longhorned tick). We targeted the 3'-UTR of a novel endochitinase-like gene (HlemCHT) identified previously in H. longicornis eggs for dsRNA-mediated gene activation. Our results showed an increased gene expression in eggs of H. longicornis endochitinase-dsRNA-injected (dsHlemCHT) ticks on day-13 post-oviposition. Furthermore, we observed that eggs of dsHlemCHT ticks exhibited relatively early egg development and hatching, suggesting a dsRNA-mediated activation of the HlemCHT gene in the eggs. This is the first attempt to provide evidence of RNAa in ticks. Although further studies are required to elucidate the detailed mechanism by which RNAa occurs in ticks, the outcome of this study provides new opportunities for the use of RNAa as a gene overexpression tool in future studies on tick biology, to reduce the global burden of ticks and tick-borne diseases.
Rapid detection of alveolar echinococcosis in hepatic nodules of horses by recombinase polymerase amplification assay. 国際誌査読有り

Tatsuro Hifumi, Tetsuya Tanaka, Miho Sato, Kohei Akioka, Chiaki Fujimata, Noriaki Miyoshi

Veterinary and animal science　20　100291-100291　2023年6月

DOI： 10.1016/j.vas.2023.100291 　

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Alveolar echinococcosis in slaughtered horses remains a public health issue. This study aimed to develop a Recombinase polymerase amplification (RPA) assay targeting the mitochondrial NADH dehydrogenase subunit 5 (Nad5) gene of Echinococcus multilocularis for the rapid detection of equine alveolar echinococcosis. Thirty-six hepatic solid nodules obtained from each horse (n = 36) were evaluated based on histopathological examination and Nad5-targeted PCR and then submitted to the RPA assay. The results of the developed RPA assay were 94.4% consistent with those of Nad5 PCR and Cohen's kappa coefficient value was 0.89 statistically, indicating high agreement. In addition, the RPA assay using the plasmid samples was one hundredfold more sensitive than PCR testing. Consequently, these results suggest that the performance of the RPA assay developed in this study is equal to that of conventional PCR testing.
Phylogenetic Position of Haemaphysalis kashmirensis and Haemaphysalis cornupunctata, with Notes on Rickettsia spp. 国際誌査読有り

Shah Masood Khan, Mehran Khan, Abdulaziz Alouffi, Mashal M Almutairi, Muhmmad Numan, Shafi Ullah, Muhammad Kashif Obaid, Zia Ul Islam, Haroon Ahmed, Tetsuya Tanaka, Abid Ali

Genes　14　(2)　2023年1月30日

DOI： 10.3390/genes14020360 　

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Despite high diversity in the Oriental region, ticks of the genus Haemaphysalis have been neglected regarding their genetic data and vector potential. This study aimed to genetically characterize three species of the genus Haemaphysalis: Haemaphysalis cornupunctata, Haemaphysalis kashmirensis, and Haemaphysalis montgomeryi infesting goats and sheep, and Rickettsia spp. associated with these tick species in the Hindu Kush Himalayan range of Pakistan. Altogether, 834 ticks were collected by examining 120 hosts including goats (64/120, 53.3%) and sheep (56/120, 46.6%), in which 86 (71.6%) hosts were found to be tick-infested. The morphologically identified ticks were subjected to DNA extraction and PCR for the amplification of partial 16S rDNA and cox fragments. Rickettsia spp. associated with the collected ticks were detected through the amplification of gltA, ompA and ompB partial fragments. The 16S rDNA of H. cornupunctata and H. montgomeryi showed a maximum identity of 100% with the sequences of the same species, whereas the 16S rDNA of H. kashmirensis showed the highest identity of 93-95% with Haemaphysalis sulcata. The cox sequence of H. montgomeryi displayed 100% identity with the same species. In comparison, the cox sequences of H. cornupunctata and H. kashmirensis showed maximum identities of 87.65-89.22% with Haemaphysalis punctata and 89.34% with H. sulcata, respectively. The gltA sequence of Rickettsia sp. from H. kashmirensis showed the highest identity of 97.89% with Rickettsia conorii subsp. raoultii, while the ompA and ompB fragments from the same DNA samples revealed 100% and 98.16% identity with Rickettsia sp. and "Candidatus Rickettsia longicornii", respectively. Another gltA sequence amplified from H. montgomeryi ticks showed 100% identity with Rickettsia hoogstraalii, while the attempts to amplify ompA and ompB for R. hoogstraalii were unsuccessful. In the phylogenetic tree, the 16S rDNA of H. cornupunctata clustered with the corresponding species while its cox clustered with H. punctata. Both 16S rDNA and cox sequences of H. kashmirensis clustered with H. sulcata. The gltA sequence of Rickettsia sp. was clustered individually in the spotted fever (SF) group of Rickettsia, while the gltA sequence of R. hoogstraalii was clustered with the same species in the transition group of Rickettsia. In the SF group, the rickettsial ompA and ompB sequence clustered with undetermined Rickettsia sp. and "Candidatus Rickettsia longicornii", respectively. This is the earliest study regarding the genetic characterization of H. kashmirensis. This study indicated that ticks belong to the genus Haemaphysalis have the potential of harboring and/or transmitting Rickettsia spp. in the region.
Localization of secreted ferritin (FER2) in the embryos of the tick Haemaphysalis longicornis. 国際誌査読有り

Emmanuel Pacia Hernandez, Kei Shimazaki, Hiroko Niihara, Rika Umemiya-Shirafuji, Kozo Fujisaki, Tetsuya Tanaka

Parasites & vectors　16　(1)　42-42　2023年1月30日

DOI： 10.1186/s13071-023-05669-5 　

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Despite the absence of a blood meal, embryogenesis involves many processes that require nutrients and other essential elements, including iron. Due to the lack of an external source of these nutrients, these requirements are acquired maternally. Because of the toxic nature of iron, they are transferred through iron transport molecules such as secreted ferritin (FER2). Here we tried to follow the trail of the FER2 through indirect immunofluorescence, and we observed an apparent shift of FER2 from the germ layer at the early part of development to the appendages during the late stage of embryogenesis. FER2 is also found in the middle part of the legs of the embryo. The apparent movement not only sheds light on iron processing events during embryogenesis but also indirectly guides organogenesis in the tick.
First record of Alectorobius coniceps (Ixodoidea: Argasidae) and Dermacentor sp. (Ixodoidea: Ixodidae) in Pakistan. 国際誌査読有り

Abid Ali, Mehran Khan, Zafar Ullah, Muhammad Numan, Kun-Hsien Tsai, Abdulaziz Alouffi, Mashal M Almutairi, Tetsuya Tanaka

Frontiers in veterinary science　10　1326734-1326734　2023年

DOI： 10.3389/fvets.2023.1326734 　

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Alectorobius species are soft ticks primarily infesting birds, such as swallows, while Dermacentor species are hard ticks mainly infesting mammals, such as small ruminants. This study for the first time reported on the morphological and molecular bases of two tick species, namely A. coniceps and a Dermacentor sp. in Pakistan. The former species was examined in swallows' nests in Khyber Pakhtunkhwa province, while the latter species was examined in small ruminants in Balochistan province. In total, 25 ticks were collected, with 14 ticks morphologically identified as A. coniceps (males = 9 and females = 5) and 11 ticks identified as Dermacentor sp. (males = 7 and females = 4). Following morphological identification, molecular identification was gained by obtaining 16S rDNA and cox1 sequences for these ticks. The BLAST results for the 16S rDNA and cox1 sequences from A. coniceps shared a maximum identity of 97.46% and 96.49% with the same species from Malta. The BLAST analysis of the 16S rDNA and cox1 sequences from Dermacentor sp. showed maximum identities of 98.42% and 97.45% with Dermacentor pavlovskyi from China. The phylogenetic analysis based on 16S rDNA and cox1 of A. coniceps showed a close evolutionary relationship with the same species. The case of Dermacentor sp., based on 16S DNA and cox1, indicated a close evolutionary relationship with Dermacentor pavlovskyi from China.
First report on detection of Hepatozoon ayorgbor in Rhipicephalus haemaphysaloides and Hepatozoon colubri in Haemaphysalis sulcata and Hyalomma anatolicum: risks of spillover of Hepatozoon spp. from wildlife to domestic animals. 国際誌査読有り

Hadia Tila, Mehran Khan, Mashal M Almutairi, Abdulaziz Alouffi, Haroon Ahmed, Tetsuya Tanaka, Kun-Hsien Tsai, Abid Ali

Frontiers in veterinary science　10　1255482-1255482　2023年

DOI： 10.3389/fvets.2023.1255482 　

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This study aimed to detect Hepatozoon spp. in ticks infesting asymptomatic domestic animals and to provide insight into their potential spillover from wild to domestic animals. In total, 537 tick specimens were collected in Khyber Pakhtunkhwa, Pakistan, and morphologically identified. The most prevalent tick species was Haemaphysalis cornupunctata (69; 12.8%), followed by Haemaphysalis kashmirensis (62; 11.5%), Rhipicephalus microplus (58; 10.8%), Haemaphysalis montgomeryi (51; 9.5%), Rhipicephalus sanguineus (49; 9.1%), each Haemaphysalis bispinosa and Haemaphysalis sulcata (43; 8.0%), each Hyalomma anatolicum and Rhipicephalus turanicus (37; 6.9%), Rhipicephalus haemaphysaloides (33; 6.1%) Hyalomma scupense (30; 5.6%), and Hyalomma isaaci (25; 4.7%). The extracted DNA from a subset of each tick species was subjected to PCR to amplify 18S rRNA fragments of Hepatozoon spp. By BLAST analysis, the Hepatozoon sp. detected in Hy. anatolicum infesting cows and in Ha. sulcata infesting sheep showed 99.7% maximum identity with Hepatozoon colubri. Similarly, the Hepatozoon sp. detected in R. haemaphysaloides infesting goats shared 99.49% maximum identity with Hepatozoon ayorgbor, and the Hepatozoon sp. detected in R. sanguineus infesting dogs exhibited 99.7% identity with Hepatozoon canis. Having an overall infection rate (9.3%; 16/172), the highest infection rate was recorded for each H. canis, and H. colubri (3.5%; 6/172), followed by H. ayorgbor (2.3%; 4/172). In the phylogenetic tree, H. colubri clustered with corresponding species from Iran, H. ayorgbor clustered with the same species from Croatia, Ghana, and Portugal, and H. canis clustered with the conspecifics from Iran, Israel, Romania, and Zambia. Regarding the potential spillover of Hepatozoon spp. from wildlife through ticks, free ranging animals was at higher risk compared to confined animals (RR = 3.05), animals consuming food from wildlife habitats were at higher risk compared to those consuming domestic food (RR = 3.06), and animals residing in farm buildings located in wildlife habitats were at higher risk compared to those residing in farm buildings located in villages (RR = 3.28). In addition to the first report on H. canis in R. sanguineus in Pakistan, this is the earliest data showing H. ayorgbor in R. haemaphysaloides and H. colubri in Ha. sulcata and Hy. anatolicum. These preliminary findings suggest a potential spillover of Hepatozoon spp. from wild to domestic animals via ticks under certain risk factors.
First report on tick-borne pathogens detected in ticks infesting stray dogs near butcher shops. 国際誌査読有り

Abid Ali, Shafi Ullah, Muhammad Numan, Mashal M Almutairi, Abdulaziz Alouffi, Tetsuya Tanaka

Frontiers in veterinary science　10　1246871-1246871　2023年

DOI： 10.3389/fvets.2023.1246871 　

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Public health is a major concern for several developing countries due to infectious agents transmitted by hematophagous arthropods such as ticks. Health risks due to infectious agents transmitted by ticks infesting butcher-associated stray dogs (BASDs) in urban and peri-urban regions have been neglected in several developing countries. To the best of the authors' knowledge, this is the first study assessing public health risks due to ticks infesting BASDs in Pakistan's urban and peri-urban areas. A total of 575 ticks (390 from symptomatic and 183 from asymptomatic BASDs) were collected from 117 BASDs (63 symptomatic and 54 asymptomatic); the ticks belonged to 4 hard tick species. A subset of each tick species' extracted DNA was subjected to polymerase chain reaction (PCR) to amplify the 16S rDNA and cox1 sequences of the reported tick species, as well as bacterial and protozoal agents. The ticks' 16S rDNA and cox1 sequences showed 99-100% identities, and they were clustered with the sequence of corresponding species from Pakistan and other countries in phylogenetic trees. Among the screened 271 ticks' DNA samples, Anaplasma spp. was detected in 54/271 (19.92%) samples, followed by Ehrlichia spp. (n = 40/271, 14.76%), Rickettsia spp. (n = 33/271, 12.17%), Coxiella spp. (n = 23/271, 4.48%), and Hepatozoon canis (n = 9/271, 3.32%). The obtained sequences and phylogenetic analyzes revealed that the pathogens detected in ticks were Ehrlichia minasensis, Ehrlichia sp., Hepatozoon canis, Coxiella burnetii, Coxiella sp., Anaplasma capra, Anaplasma platys, Anaplasma sp., Rickettsia massiliae, "Candidatus Rickettsia shennongii" and Rickettsia aeschlimannii. Tick-borne pathogens such as E. minasensis, H. canis, A. capra, A. platys, and R. aeschlimannii, were detected based on the DNA for the first time in Pakistan. This is the first report on public health risks due to ticks infesting BASDs. These results not only provided insights into the occurrence of novel tick-borne pathogens in the region but also revealed initial evidence of zoonotic threats to both public health and domestic life.
Molecular detection of Coxiella spp. in ticks (Ixodidae and Argasidae) infesting domestic and wild animals: with notes on the epidemiology of tick-borne Coxiella burnetii in Asia. 国際誌査読有り

Abid Ali, Muhammad Kashif Obaid, Mashal M Almutairi, Abdulaziz Alouffi, Muhammad Numan, Shafi Ullah, Gauhar Rehman, Zia Ul Islam, Sher Bahadar Khan, Tetsuya Tanaka

Frontiers in microbiology　14　1229950-1229950　2023年

DOI： 10.3389/fmicb.2023.1229950 　

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Tick-borne Coxiella spp. are emerging in novel regions infecting different hosts, but information regarding their occurrence is limited. The purpose of this study was the molecular screening of Coxiella spp. in various ticks infesting goats, sheep, camels, cattle, wild mice, and domestic fowls (Gallus gallus domesticus) in various districts of Khyber Pakhtunkhwa, Pakistan. Morphologically identified tick species were confirmed by obtaining their cox1 sequences and were molecularly screened for Coxiella spp. by sequencing GroEL fragments. Almost 345 out of 678 (50.9%) hosts were infested by nine tick species. Regarding the age groups, the hosts having an age >3 years were highly infested (192/345, 55.6%), while gender-wise infestation was higher in female hosts (237/345, 68.7%). In collected ticks, the nymphs were outnumbered (613/1,119, 54.8%), followed by adult females (293/1,119, 26.2%) and males (213/1,119, 19.7%). A total of 227 ticks were processed for molecular identification and detection of Coxiella spp. The obtained cox1 sequences of nine tick species such as Hyalomma dromedarii, Hyalomma anatolicum, Haemaphysalis cornupunctata, Haemaphysalis bispinosa, Haemaphysalis danieli, Haemaphysalis montgomeryi, Rhipicephalus haemaphysaloides, Rhipicephalus microplus, and Argas persicus showed maximum identities between 99.6% and 100% with the same species and in the phylogenetic tree, clustered to the corresponding species. All the tick species except Ha. danieli and R. microplus were found positive for Coxiella spp. (40/227, 17.6%), including Coxiella burnetii (15/40, 6.7%), Coxiella endosymbionts (14/40, 6.3%), and different Coxiella spp. (11/40, 4.9%). By the BLAST results, the GroEL fragments of Coxiella spp. showed maximum identity to C. burnetii, Coxiella endosymbionts, and Coxiella sp., and phylogenetically clustered to the corresponding species. This is the first comprehensive report regarding the genetic characterization of Coxiella spp. in Pakistan's ticks infesting domestic and wild hosts. Proper surveillance and management measures should be undertaken to avoid health risks.
Molecular detection of Bartonella and Borrelia in pet dogs in Metro Manila and Laguna, Philippines. 国際誌査読有り

Chae Eun Lee, Jeong Hee Ikeda, Mikaella Andrea M Manongdo, Dan Rica T Romerosa, Kristina Andrea C Sandalo-De Ramos, Tetsuya Tanaka, Remil L Galay

Veterinary world　16　(7)　1546-1551　2023年

DOI： 10.14202/vetworld.2023.1546-1551 　

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BACKGROUND AND AIM: Bartonella and Borrelia are zoonotic vector-borne pathogens that can infect dogs and humans. Data on Bartonella and Borrelia in dogs in the Philippines are lacking. This study was conducted to validate previous reports and further investigate the occurrence of Bartonella and Borrelia spp. in cities of Metro Manila. MATERIALS AND METHODS: A total of 182 canine blood samples were acquired with DNA using a commercial extraction kit from selected veterinary clinics in the cities of Metro Manila and Laguna, Philippines. The mammalian actin was amplified through polymerase chain reaction (PCR), followed by PCR assays targeting Bartonella gltA and Borrelia flaB. Further PCR assays targeting 16S of Borrelia and ospA and ospC of Borrelia burgdorferi were performed for those that showed flaB bands. RESULTS: A positive band for gltA of Bartonella was observed in 9 (4.95%) samples, whereas a positive band for flaB of Borrelia was observed in 15 (8.24%) samples. Subsequent PCR assays for other genes of Borrelia were negative. CONCLUSION: These results confirmed the presence of Bartonella and warranted further investigation for the possible presence of other Borrelia species.
Molecular detection and characterization of Anaplasma marginale infecting cattle, buffalo, and camel populations in southern Egypt. 国際誌査読有り

Hassan Y A H Mahmoud, Alsagher O Ali, Tetsuya Tanaka

Frontiers in veterinary science　10　1169323-1169323　2023年

DOI： 10.3389/fvets.2023.1169323 　

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Anaplasmosis is a severe tickborne disease of ruminants caused by Anaplasma marginale. A. marginale is distributed worldwide and attacks erythrocytes, resulting in an increased body temperature, anemia, jaundice, abortion, and, in some cases, death. Animals infected with this pathogen become lifelong carriers. In this study, we aimed to detect and characterize A. marginale isolated from cattle, buffalo, and camel populations using novel molecular techniques in southern Egypt. In total, 250 samples (from 100 cattle, 75 water buffaloes, and 75 camels) were analyzed by PCR for the presence of Anaplasmataceae, specifically A. marginale. The animals varied in breed, age, and gender, with most showing no signs of severe disease. By species, A. marginale was found in 61 out of 100 (61%) cattle, 9 out of 75 (12%) buffaloes, and only 5 out of 75 (6.66%) camels. All A. marginale-positive samples were examined for the heat-shock protein groEL gene and, additionally, for major surface protein 4 (msp4) and major surface protein 5 (msp5) genes to enhance specificity. Phylogenetic analysis of A. marginale targeted three genes (groEL, msp4, and msp5). This study provides the first report on using three genes for A. marginale detection in Camelus dromedarius in southern Egypt and generated new phylogenetic data for A. marginale infections in camels. A. marginale infection is endemic in different animal species in southern Egypt. Screening herds for A. marginale is recommended even when the signs of anaplasmosis are absent.
Assessment of cypermethrin and amitraz resistance and molecular profiling of voltage-gated sodium channel and octopamine tyramine genes of Rhipicephalus microplus. 国際誌査読有り

Muhammad Kashif Obaid, Mashal M Almutairi, Abdulaziz Alouffi, Sher Zaman Safi, Tetsuya Tanaka, Abid Ali

Frontiers in cellular and infection microbiology　13　1176013-1176013　2023年

DOI： 10.3389/fcimb.2023.1176013 　

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Control of ticks and tick-borne pathogens is a priority for human and animal health. Livestock-holders extensively rely on acaricide applications for tick control. Different groups of acaricides including cypermethrin and amitraz have been consistently used in Pakistan. There has been a gap in understanding the susceptibility or resistance of Rhipicephalus microplus, the most prevalent tick in Pakistan, to acaricides. The present study aimed to molecularly characterize cypermethrin and amitraz targeted genes such as voltage-gated sodium channel (VGSC) and octopamine tyramine (OCT/Tyr) of R. microplus ticks in Khyber Pakhtunkhwa (KP), Pakistan to monitor the acaricides resistance. Tick specimens were collected from cattle and buffaloes in northern (Chitral, Shangla, Swat, Dir, and Buner), central (Peshawar, Mardan, Charsadda, Swabi, and Nowshera), and southern districts (Kohat, Karak, Lakki Marwat, Tank, and Dera Ismail Khan) of KP, Pakistan. Different concentrations of commercially available cypermethrin (10%) and amitraz (12.5%) were prepared for in vitro larval immersion tests (LIT). In LIT, the average mortality rate of immersed larvae was recorded that was increased gradually with an increase in the concentration of specific acaricide. The larvae's highest mortality rates (94.5% and 79.5%) were observed at 100-ppm of cypermethrin and amitraz, respectively. A subset of 82 R. microplus ticks was subjected to extract genomic DNA, followed by PCR to amplify partial fragments of VGSC (domain-II) and OCT/Tyr genes. The BLAST results of the consensus sequence of VGSC gene (domain-II) showed 100% identity with the acaricides susceptible tick sequence from the United States (reference sequence). Obtained identical sequences of OCT/Tyr genes showed maximum identity (94-100%) with the identical sequences reported from Australia (reference sequence), India, Brazil, Philippines, USA, South Africa, and China. Thirteen single nucleotide polymorphisms (10 synonymous and three non-synonymous) were observed at various positions of partial OCT/Tyr gene fragments. The SNP at position A-22-C (T-8-P) in OCT/Tyr gene has been linked to amitraz resistance in R. microplus ticks. Molecular analysis and LIT bioassay's findings indicate the availability of resistant R. microplus ticks in the KP region. To our understanding, this is the first preliminary study to monitor cypermethrin and amitraz resistance via molecular profiling of cypermethrin and amitraz targeted genes (VGSC and OCT/Tyr) in combination with in vitro bioassays (LIT) in R. microplus ticks from Pakistan.
Clinical and Pathological Findings in Fatal Cases of Severe Fever With Thrombocytopenia Syndrome With High Viremia in Cats. 国際誌査読有り

Aya Matsuu, Hitoshi Hatai, Tatsuro Hifumi, Emu Hamakubo, Maho Take, Tetsuya Tanaka, Yasuyuki Momoi, Yasuyuki Endo, Ai Koyoshi, Yuka Kamikubo, Raye Kamatsuki, Natsuki Kurusu, Haruna Tabata, Hiroshi Matsuyama, Mei Yanaidani, Michiyo Kawabata, Takahiro Kawabata

Topics in companion animal medicine　52　100756-100756　2023年

DOI： 10.1016/j.tcam.2022.100756 　

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Severe fever with thrombocytopenia syndrome (SFTS) is an emerging zoonotic disease caused by the SFTS virus (SFTSV). SFTSV causes severe symptoms both in humans and cats. In this study, we report the clinical and pathological findings of 4 fatal cases of cats with high SFTS viremia levels. These cats showed an acute onset of fever, leukopenia, thrombocytopenia, and increased serum amyloid A and pro-inflammatory cytokine levels. A high viral copy number was detected in the blood, oral swabs, rectal swabs, conjunctiva swabs, and urine. Histopathologically, necrotizing lymphadenitis, splenitis with lymphoblastoid cell proliferation, and hemophagocytosis were observed in all 4 cats. Immunohistochemistry revealed the presence of SFTSV antigen on lymphoblastoid B cells. SFTSV-RNA was detected in systemic tissues, including the brain. The present findings provide useful information for understanding the features of fatal SFTS in cats. To elucidate the mechanisms of severe progress of SFTS cats, as well as its role as a source of human infection, further research is needed.
Editorial: Methods and applications in invertebrate physiology. 国際誌査読有り

Natraj Krishnan, Tetsuya Tanaka, Graziano Fiorito, Fernando Ariel Genta, Pamela Imperadore

Frontiers in physiology　14　1158972-1158972　2023年

DOI： 10.3389/fphys.2023.1158972 　
A novel eukaryotic RdRP-dependent small RNA pathway represses antiviral immunity by controlling an ERK pathway component in the black-legged tick. 国際誌査読有り

Canran Feng, Kyosuke Torimaru, Mandy Yu Theng Lim, Li-Ling Chak, Masami Shiimori, Kosuke Tsuji, Tetsuya Tanaka, Junko Iida, Katsutomo Okamura

PloS one　18　(3)　e0281195　2023年

DOI： 10.1371/journal.pone.0281195 　

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Small regulatory RNAs (sRNAs) are involved in antiviral defense and gene regulation. Although roles of RNA-dependent RNA Polymerases (RdRPs) in sRNA biology are extensively studied in nematodes, plants and fungi, understanding of RdRP homologs in other animals is still lacking. Here, we study sRNAs in the ISE6 cell line, which is derived from the black-legged tick, an important vector of human and animal pathogens. We find abundant classes of ~22nt sRNAs that require specific combinations of RdRPs and sRNA effector proteins (Argonautes or AGOs). RdRP1-dependent sRNAs possess 5'-monophosphates and are mainly derived from RNA polymerase III-transcribed genes and repetitive elements. Knockdown of some RdRP homologs misregulates genes including RNAi-related genes and the regulator of immune response Dsor1. Sensor assays demonstrate that Dsor1 is downregulated by RdRP1 through the 3'UTR that contains a target site of RdRP1-dependent repeat-derived sRNAs. Consistent with viral gene repression by the RNAi mechanism using virus-derived small interfering RNAs, viral transcripts are upregulated by AGO knockdown. On the other hand, RdRP1 knockdown unexpectedly results in downregulation of viral transcripts. This effect is dependent on Dsor1, suggesting that antiviral immunity is enhanced by RdRP1 knockdown through Dsor1 upregulation. We propose that tick sRNA pathways control multiple aspects of immune response via RNAi and regulation of signaling pathways.
Description of Male, Redescription of Female, Host Record, and Phylogenetic Position of Haemaphysalis danieli. 国際誌査読有り

Iftikhar Ahmad, Shafi Ullah, Abdulaziz Alouffi, Mashal M Almutairi, Mehran Khan, Muhammad Numan, Sher Zaman Safi, Lidia Chitimia-Dobler, Tetsuya Tanaka, Abid Ali

Pathogens (Basel, Switzerland)　11　(12)　2022年12月8日

DOI： 10.3390/pathogens11121495 　

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Haemaphysalis ticks are globally distributed with the greatest diversity in the Oriental region. This study aimed to primarily provide information on the morphology, host record, and preliminary phylogenetic position of a poorly known tick Haemaphysalis danieli. Herds comprised of goats and sheep were examined for this tick species in Upper Dir, Khyber Pakhtunkhwa, Pakistan. A total of 127 ticks, including males (n = 15, 11.8%) and females (n = 112, 88.2%), were collected, and morphologically identified as H. danieli. The morphological identification was confirmed through the 16S rDNA and cytochrome c oxidase (cox1) sequences. Phylogenetic analysis inferred based on 16S rDNA and cox1 showed a close evolutionary relationship of H. danieli with a conspecific from China and an undetermined Haemaphysalis sp. from China and Anatolia. A total of 32/223 (14.3%) goats in two different herds were the only host infested by H. danieli. The earliest study provided the morphological description of H. danieli male, host record, and phylogenetic position. The information provided herein could assist in minimizing the knowledge gap regarding the systematic and taxonomy of Haemaphysalis species.
Low Genetic Polymorphism in the Immunogenic Sequences of Rhipicephalus microplus Clade C. 国際誌査読有り

Ismail Zeb, Mashal M Almutairi, Abdulaziz Alouffi, Nabila Islam, Luís Fernando Parizi, Sher Zaman Safi, Tetsuya Tanaka, Itabajara da Silva Vaz Jr, Abid Ali

Vaccines　10　(11)　2022年11月11日

DOI： 10.3390/vaccines10111909 　

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Rhipicephalus microplus tick highly affects the veterinary sector throughout the world. Different tick control methods have been adopted, and the identification of tick-derived highly immunogenic sequences for the development of an anti-tick vaccine has emerged as a successful alternate. This study aimed to characterize immunogenic sequences from R. microplus ticks prevalent in Pakistan. Ticks collected in the field were morphologically identified and subjected to DNA and RNA extraction. Ticks were molecularly identified based on the partial mitochondrial cytochrome C oxidase subunit (cox) sequence and screened for piroplasms (Theileria/Babesia spp.), Rickettsia spp., and Anaplasma spp. PCR-based pathogens-free R. microplus-derived cDNA was used for the amplification of full-length cysteine protease inhibitor (cystatin 2b), cathepsin L-like cysteine proteinase (cathepsin-L), glutathione S-transferase (GST), ferritin 1, 60S acidic ribosomal protein (P0), aquaporin 2, ATAQ, and R. microplus 05 antigen (Rm05Uy) coding sequences. The cox sequence revealed 100% identity with the nucleotide sequences of Pakistan's formerly reported R. microplus, and full-length immunogenic sequences revealed maximum identities to the most similar sequences reported from India, China, Cuba, USA, Brazil, Egypt, Mexico, Israel, and Uruguay. Low nonsynonymous polymorphisms were observed in ATAQ (1.5%), cathepsin-L (0.6%), and aquaporin 2 (0.4%) sequences compared to the homologous sequences from Mexico, India, and the USA, respectively. Based on the cox sequence, R. microplus was phylogenetically assembled in clade C, which includes R. microplus from Pakistan, Myanmar, Malaysia, Thailand, Bangladesh, and India. In the phylogenetic trees, the cystatin 2b, cathepsin-L, ferritin 1, and aquaporin 2 sequences were clustered with the most similar available sequences of R. microplus, P0 with R. microplus, R. sanguineus and R. haemaphysaloides, and GST, ATAQ, and Rm05Uy with R. microplus and R. annulatus. This is the first report on the molecular characterization of clade C R. microplus-derived immunogenic sequences.
Spatio-Temporal Patterns of Ticks and Molecular Survey of Anaplasma marginale, with Notes on Their Phylogeny. 国際誌査読有り

Shumaila Alam, Mehran Khan, Abdulaziz Alouffi, Mashal M Almutairi, Shafi Ullah, Muhammad Numan, Nabila Islam, Zaibullah Khan, Ome Aiman, Sher Zaman Safi, Tetsuya Tanaka, Abid Ali

Microorganisms　10　(8)　2022年8月17日

DOI： 10.3390/microorganisms10081663 　

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Hard ticks (Ixodida: Ixodidae) are medically important ectoparasites that feed on all classes of terrestrial vertebrates. Recently, we molecularly characterized hard ticks and associated Anaplasma spp. in the northern and central regions of Khyber Pakhtunkhwa (KP), Pakistan; however, this knowledge was missing in the southern regions. This study aimed to investigate tick prevalence, host range, genetic diversity, and molecular survey of Anaplasma spp. in a wide range of tick species in two distinct physiographic regions of southern KP. A total of 1873 hard ticks were randomly collected from 443/837 hosts (cattle, Asian water buffaloes, horses, goats, sheep, dogs, and camels) in Lakki Marwat, Bannu, and Orakzai districts of KP. Overall, 12 tick species were morphologically identified, among which Hyalomma dromedarii was the most prevalent species (390/1873, 20.9%), followed by Hy. anatolicum (294, 15.7%), Rhipicephalus microplus (262, 14%), Hy. scupense (207, 11.1%), R. sanguineus (136, 7.3%), R. turanicus (121, 6.5%), Haemaphysalis cornupunctata (107, 5.7%), R. haemaphysaloides (110, 5.9%), Ha. montgomeryi (87, 4.6%), Hy. isaaci (58, 3.1%), Ha. bispinosa (54, 2.9%), and Ha. sulcata (47, 2.5%). The extracted DNA from a subset of each tick species was subjected to PCR to amplify cox1 or 16S rRNA sequences of ticks and 16S rRNA sequences of Anaplasma spp. The tick cox1 sequences showed 99-100% identities with the sequences of the same species, whereas 16S rRNA sequences of R. turanicus, Ha. montgomeryi and Ha. sulcata showed 97-100% identities with the corresponding species. The 16S rRNA sequence of Ha. cornupunctata showed 92% identity with the species from the same subgenus, such as Ha. punctata. The 16S rRNA sequence of Anaplasma spp. showed 100% identity with Anaplasma marginale. Moreover, 54 ticks were found positive for A. marginale with a total infection rate of 17.2%. The highest infection rate was recorded in Hy. dromedarii (31.1%) and the lowest in each R. haemaphysaloides and R. sanguineus (20%). All the cox1 or 16S rRNA sequences in phylogenetic trees clustered with the same species, except Ha. cornupunctata, which clustered with the Ha. (Aboimisalis) punctata. In this study, Ha. cornupunctata was reported for the first time at the molecular level. The genetic characterization of ixodid ticks and molecular detection of associated A. marginale will assist in the epidemiological surveillance of these parasites in the region.
Molecular Characterization and Assessment of Risk Factors Associated with Theileria annulata Infection. 国際誌査読有り

Karim Ullah, Muhammad Numan, Abdulaziz Alouffi, Mashal M Almutairi, Hafsa Zahid, Majid Khan, Zia Ul Islam, Atif Kamil, Sher Zaman Safi, Haroon Ahmed, Tetsuya Tanaka, Abid Ali

Microorganisms　10　(8)　2022年8月9日

DOI： 10.3390/microorganisms10081614 　

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Theileria annulata is a tick-associated parasite that causes tropical theileriosis in livestock and is responsible for huge economic losses. Studies have been neglected on the effect of Theileria spp. on cattle in Khyber Pakhtunkhwa (KP), Pakistan. The present study was designed to determine the genetic diversity and assess the risk factors associated with Theileria infection in selected districts of KP. Information on the risk factors related to the Theileria infection was collected through a questionnaire. Blood samples were collected from symptomatic cattle from January 2019 to February 2020, identified morphologically through microscopic examination, and processed for molecular characterization using the 18S rRNA gene as a genetic marker. Of the 555 cattle examined (136, 24.5%) and (294, 53%) were found positive for Theileria spp. by microscopic examination and a PCR test, respectively. Based on the PCR test, the highest prevalence of infection was found in district Upper Dir (46/75, 61.3%), followed by Lower Dir (54/90, 60%); Malakand (51/88, 57.9%); Peshawar (40/80, 50%); and Charsadda (52/112, 46.4%), with the lowest in Bajaur (51/110, 46.34%). A BLAST analysis of the 18S rDNA sequences showed 99.5% identity with T. annulata. In a phylogenetic tree, the 18S rDNA sequence of T. annulata clustered with sequences from Pakistan, China, and Italy. A significant association was observed between the prevalence of infection and different host characteristics. The highest infection was found in adult cattle (216/360, 60%); females (218/377, 57.8%); and Holstein Friesian (120/180, 66.6%). Theileria infection was significantly associated with management practices. Higher infection rates were observed in free-grazing cattle (190/412, 42.2%); those kept in unhygienic conditions (246/405, 60.7%); cattle in combined farming systems (165/255, 64.8%); and those in congested stall systems (150/218, 68.8%). Seasonal patterns were found to be significantly associated with infection, and a higher infection rate was observed in summer (215/350, 61.4%) than in winter (79/205, 38.5%). Identified risk factors should be considered in designing practical control approaches to reduce the burden of Theileria infection. Large scale studies are required to explore the diversity of Theileria species in KP, Pakistan.
Morphological identification and molecular characterization of economically important ticks (Acari: Ixodidae) from North and North-Western Egypt. 国際誌査読有り

Haytham Senbill, Tetsuya Tanaka, Donia Karawia, Sahidur Rahman, Jehan Zeb, Olivier Sparagano, Aiswarya Baruah

Acta tropica　231　106438-106438　2022年7月

DOI： 10.1016/j.actatropica.2022.106438 　

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Ticks represent a major source of growing economic and public health concern, especially in the tropics and sub-tropics. Towards evidencing ticks' invasion in the North and North-Western parts of Egypt, the present study aimed to investigate the morpho-molecular aspects of those ectoparasites using stereomicroscopy, scanning electron microscopy (SEM) and sequencing of the mitochondrial 16S ribosomal DNA gene (rDNA) and nuclear second internal transcribed spacer (ITS2). Our analysis confirmed the presence and well-distribution of Rhipicephalus sanguineus s.l. infesting dogs and sheep (Alexandria governorate), Rhipicephalus annulatus infesting cattle (Beheira governorate), and Hyalomma dromedarii infesting camels (Marsa Matruh governorate) from North/North-Western Egypt. 16S rDNA and ITS2 sequences of the ticks were amplified using universal and gene-specific sets of primers, sequenced and analyzed. Lengths of amplified 16S rDNA sequences in all examined tick species were found to be similar in size (approximately 460 bp); however, they differed in base pair constitutions, whereas ITS2 lengths were 1,500 bp, 1,550 bp, and 1,800 bp for Rh. annulatus, Rh. sanguineus s.l., and Hy. dromedarii, respectively. Phylogenetically, based on the 16S rDNA results, Rh. sanguineus s.l. ticks clustered with the southeastern Europe lineage from Romania and Greece, Rh. annulatus ticks were similar to Turkish populations, and Hy. dromedarii were close to the isolates from Tunisia. Similarly, based on ITS2 sequences, Rh. sanguineus s.l. from dogs were showing 99% similarity to Nigerian populations; however, those collected from sheep were closer to Iranian populations with 4.1% nucleotide divergence between the two populations of different hosts. Rh. annulatus ticks were identical to a population from Romania, whereas Hy. dromedarii was close by 99.7% similarity to a population from Kenya. This is the first study reporting nucleotide sequences of 16S rDNA and ITS2 in integration with morphological identification of ticks from this part of Egypt.
Molecular Survey and Genetic Characterization of Anaplasma marginale in Ticks Collected from Livestock Hosts in Pakistan. 国際誌査読有り

Zaibullah Khan, Shehla Shehla, Abdulaziz Alouffi, Muhammad Kashif Obaid, Alam Zeb Khan, Mashal M Almutairi, Muhammad Numan, Ome Aiman, Shumaila Alam, Shafi Ullah, Sher Zaman Safi, Tetsuya Tanaka, Abid Ali

Animals : an open access journal from MDPI　12　(13)　2022年7月1日

DOI： 10.3390/ani12131708 　

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Ticks transmit pathogens to animals and humans more often than any other arthropod vector. The rural economy of Pakistan mainly depends on livestock farming, and tick infestations cause severe problems in this sector. The present study aimed to molecularly characterize the Anaplasma spp. in hard ticks collected from six districts of Khyber Pakhtunkhwa, Pakistan. Ticks were collected from various livestock hosts, including cattle breeds (Holstein-Friesian, Jersey, Sahiwal, and Achai), Asian water buffaloes, sheep, and goats from March 2018 to February 2019. Collected ticks were morphologically identified and subjected to molecular screening of Anaplasma spp. by amplifying 16S rDNA sequences. Six hundred seventy-six ticks were collected from infested hosts (224/350, 64%). Among the nine morphologically identified tick species, the highest occurrence was noted for Rhipicephalus microplus (254, 37.6%), followed by Hyalomma anatolicum (136, 20.1%), Rhipicephalus haemaphysaloides (119, 17.6%), Rhipicephalus turanicus (116, 17.1%), Haemaphysalis montgomeryi (14, 2.1%), Hyalomma dromedarii (11, 1.6%), Haemaphysalis bispinosa (10, 1.5%), Hyalomma scupense (8, 1.2%), and Haemaphysalis kashmirensis (8, 1.2%). The occurrence of tick females was highest (260, 38.5%), followed by nymphs (246, 36.4%) and males (170, 25.1%). Overall, the highest occurrence of ticks was recorded in the Peshawar district (239, 35.3%), followed by Mardan (183, 27.1%), Charsadda (110, 16.3%), Swat (52, 7.7%), Shangla (48, 7.1%), and Chitral (44, 6.5%). Among these ticks, Anaplasma marginale was detected in R. microplus, R. turanicus, and R. haemaphysaloides. The 16S rDNA sequences showed high identity (98-100%) with A. marginale reported from Australia, China, Japan, Pakistan, Thailand, Uganda, and the USA. In phylogenetic analysis, the sequence of A. marginale clustered with the same species reported from Australia, China, Pakistan, Thailand, Uruguay, and the USA. Further molecular work regarding the diversity of tick species and associated pathogens is essential across the country.
Ambivalent Roles of Oxidative Stress in Triangular Relationships among Arthropod Vectors, Pathogens and Hosts. 国際誌査読有り

Emmanuel Pacia Hernandez, Anisuzzaman, Md Abdul Alim, Hayato Kawada, Kofi Dadzie Kwofie, Danielle Ladzekpo, Yuki Koike, Takahiro Inoue, Sana Sasaki, Fusako Mikami, Makoto Matsubayashi, Tetsuya Tanaka, Naotoshi Tsuji, Takeshi Hatta

Antioxidants (Basel, Switzerland)　11　(7)　2022年6月25日

DOI： 10.3390/antiox11071254 　

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Blood-feeding arthropods, particularly ticks and mosquitoes are considered the most important vectors of arthropod-borne diseases affecting humans and animals. While feeding on blood meals, arthropods are exposed to high levels of reactive oxygen species (ROS) since heme and other blood components can induce oxidative stress. Different ROS have important roles in interactions among the pathogens, vectors, and hosts. ROS influence various metabolic processes of the arthropods and some have detrimental effects. In this review, we investigate the various roles of ROS in these arthropods, including their innate immunity and the homeostasis of their microbiomes, that is, how ROS are utilized to maintain the balance between the natural microbiota and potential pathogens. We elucidate the mechanism of how ROS are utilized to fight off invading pathogens and how the arthropod-borne pathogens use the arthropods' antioxidant mechanism to defend against these ROS attacks and their possible impact on their vector potentials or their ability to acquire and transmit pathogens. In addition, we describe the possible roles of ROS in chemical insecticide/acaricide activity and/or in the development of resistance. Overall, this underscores the importance of the antioxidant system as a potential target for the control of arthropod and arthropod-borne pathogens.
Molecular Detection and Characterization of Babesia and Theileria in Cattle and Water Buffaloes from Southern Luzon, Philippines. 国際誌査読有り

Ian Cary B Prado, Larry Xerxes B Capuno Jr, Princess Dlp Collera, Aaron Paul D Cabralda, Kristina Andrea S De Ramos, John Michael G Bernardo, Billy P Divina, Tatsunori Masatani, Tetsuya Tanaka, Remil L Galay

Microorganisms　10　(4)　2022年3月22日

DOI： 10.3390/microorganisms10040678 　

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Babesia and Theileria are tick-borne protozoan parasites that can cause significant economic losses in the cattle industry. This study aimed to contribute to the limited epidemiological data on Theileria orientalis as well as Babesia bigemina and B. bovis in large ruminants in the Philippines. Blood samples of 412 cattle and 108 water buffalo collected from four provinces in Southern Luzon, Philippines, were initially tested for the 18S rRNA gene of piroplasms through nested PCR. Positive samples were further subjected to species-specific PCR. The 18s rRNA of piroplasms was detected in 123 (29.9%) cattle and three (2.8%) water buffaloes. Theileria orientalis was found to be the most common piroplasm in cattle with a detection rate of 17.5%, followed by Babesia bovis and B. bigemina. Co-infections were also observed. Two water buffaloes were found infected with B. bovis, while one was positive for B. bigemina. The phylogenetic tree for B. bovis showed clustering of the isolates in two clades together with isolates from other countries, and a third separate clade. Meanwhile, the T. orientalis isolates in this study were distributed in three clades together with reported isolates from other countries. This study confirms the presence of T. orientalis in the Philippines and reports the genetic diversity of B. bovis and T. orientalis.
Rapid Spread of Severe Fever with Thrombocytopenia Syndrome Virus by Parthenogenetic Asian Longhorned Ticks. 国際誌国際共著査読有り

Xing Zhang, Chaoyue Zhao, Chaoyuan Cheng, Guogang Zhang, Tao Yu, Kevin Lawrence, Hongyue Li, Jimin Sun, Zeyu Yang, Ling Ye, Hongliang Chu, Ying Wang, Xiaohu Han, Yongchao Jia, Shuozhang Fan, Hirotaka Kanuka, Tetsuya Tanaka, Cheryl Jenkins, Kristene Gedye, Shona Chandra, Dana C Price, Qiyong Liu, Young Ki Choi, Xiangjiang Zhan, Zhibin Zhang, Aihua Zheng

Emerging infectious diseases　28　(2)　363-372　2022年2月

DOI： 10.3201/eid2802.211532 　

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Severe fever with thrombocytopenia syndrome virus (SFTSV) is spreading rapidly in Asia. This virus is transmitted by the Asian longhorned tick (Haemaphysalis longicornis), which has parthenogenetically and sexually reproducing populations. Parthenogenetic populations were found in ≥15 provinces in China and strongly correlated with the distribution of severe fever with thrombocytopenia syndrome cases. However, distribution of these cases was poorly correlated with the distribution of populations of bisexual ticks. Phylogeographic analysis suggested that the parthenogenetic population spread much faster than bisexual population because colonization is independent of sexual reproduction. A higher proportion of parthenogenetic ticks was collected from migratory birds captured at an SFTSV-endemic area, implicating the contribution to the long-range movement of these ticks in China. The SFTSV susceptibility of parthenogenetic females was similar to that of bisexual females under laboratory conditions. These results suggest that parthenogenetic Asian longhorned ticks, probably transported by migratory birds, play a major role in the rapid spread of SFTSV.
Molecular Survey and Spatial Distribution of Rickettsia spp. in Ticks Infesting Free-Ranging Wild Animals in Pakistan (2017-2021). 国際誌査読有り

Abid Ali, Shehla Shehla, Hafsa Zahid, Farman Ullah, Ismail Zeb, Haroon Ahmed, Itabajara da Silva Vaz Jr, Tetsuya Tanaka

Pathogens (Basel, Switzerland)　11　(2)　2022年1月26日

DOI： 10.3390/pathogens11020162 　

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Rickettsia spp. associated with ticks infesting wild animals have been mostly neglected in several countries, including Pakistan. To address this knowledge gap, ticks were collected during 2017 to 2021 from wild animals including cats (Felis chaus), Indian hedgehogs (Paraechinus micropus), and wild boars (Sus scrofa). The collected ticks were morpho-molecularly identified and screened for the detection of Rickettsia spp. Morphologically identified ticks were categorized into four species of the genus Rhipicephalus: Rhipicephalus haemaphysaloides, Rh. turanicus, Rh. sanguineus sensu lato (s.l), and Rh. microplus. Among 53 wild animals examined, 31 were infested by 531 ticks, an overall prevalence of 58.4%. Adult female ticks were predominant (242 out of 513 ticks collected, corresponding to 46%) in comparison with males (172, 32%), nymphs (80, 15%) and larvae (37, 7%). The most prevalent tick species was Rh. turanicus (266, 50%), followed by Rh. microplus (123, 23%), Rh. sanguineus (106, 20%), and Rh. haemaphysaloides (36, 7%). Among the screened wild animals, wild boars were the most highly infested, with 268 ticks being collected from these animals (50.4%), followed by cats (145, 27.3%) and hedgehogs (118, 22.3%). Tick species Rh. haemaphysaloides, Rh. turanicus, and Rh. sanguineus were found on wild boars, Rh. haemaphysaloides, and Rh. microplus on cats, and Rh. turanicus on hedgehogs. In a phylogenetic analysis, mitochondrial cytochrome C oxidase 1 (cox1) sequences obtained from a subsample (120) of the collected ticks clustered with sequences from Bangladesh, China, India, Iran, Myanmar, and Pakistan, while 16S ribosomal DNA (16S rDNA) sequences clustered with sequences reported from Afghanistan, Egypt, India, Pakistan, Romania, Serbia, and Taiwan. Among Rickettsia infected ticks (10/120, 8.3%), Rh. turanicus (7/10, 70%), and Rh. haemaphysaloides (3/10, 30%) were found infesting wild boars in the districts Mardan and Charsadda. The obtained rickettsial gltA gene sequences showed 99% and ompA gene sequences showed 100% identity with Rickettsia massiliae, and the phylogenetic tree shows ompA clustered with the same species reported from France, Greece, Spain, and USA. This study emphasizes the need for effective surveillance and control programs in the region to prevent health risks due to tick-borne pathogens, and that healthy infested wild animals may play a role in the spread of these parasites.
Host Immune Responses to Salivary Components - A Critical Facet of Tick-Host Interactions. 国際誌査読有り

Abid Ali, Ismail Zeb, Abdulaziz Alouffi, Hafsa Zahid, Mashal M Almutairi, Fahdah Ayed Alshammari, Mohammed Alrouji, Carlos Termignoni, Itabajara da Silva Vaz Jr, Tetsuya Tanaka

Frontiers in cellular and infection microbiology　12　809052-809052　2022年

DOI： 10.3389/fcimb.2022.809052 　

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Tick sialome is comprised of a rich cocktail of bioactive molecules that function as a tool to disarm host immunity, assist blood-feeding, and play a vibrant role in pathogen transmission. The adaptation of the tick's blood-feeding behavior has lead to the evolution of bioactive molecules in its saliva to assist them to overwhelm hosts' defense mechanisms. During a blood meal, a tick secretes different salivary molecules including vasodilators, platelet aggregation inhibitors, anticoagulants, anti-inflammatory proteins, and inhibitors of complement activation; the salivary repertoire changes to meet various needs such as tick attachment, feeding, and modulation or impairment of the local dynamic and vigorous host responses. For instance, the tick's salivary immunomodulatory and cement proteins facilitate the tick's attachment to the host to enhance prolonged blood-feeding and to modulate the host's innate and adaptive immune responses. Recent advances implemented in the field of "omics" have substantially assisted our understanding of host immune modulation and immune inhibition against the molecular dynamics of tick salivary molecules in a crosstalk between the tick-host interface. A deep understanding of the tick salivary molecules, their substantial roles in multifactorial immunological cascades, variations in secretion, and host immune responses against these molecules is necessary to control these parasites. In this article, we reviewed updated knowledge about the molecular mechanisms underlying host responses to diverse elements in tick saliva throughout tick invasion, as well as host defense strategies. In conclusion, understanding the mechanisms involved in the complex interactions between the tick salivary components and host responses is essential to decipher the host defense mechanisms against the tick evasion strategies at tick-host interface which is promising in the development of effective anti-tick vaccines and drug therapeutics.
Acaricides Resistance in Ticks: Selection, Diagnosis, Mechanisms, and Mitigation. 国際誌査読有り

Muhammad Kashif Obaid, Nabila Islam, Abdulaziz Alouffi, Alam Zeb Khan, Itabajara da Silva Vaz Jr, Tetsuya Tanaka, Abid Ali

Frontiers in cellular and infection microbiology　12　941831-941831　2022年

DOI： 10.3389/fcimb.2022.941831 　

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Ticks are blood-feeding ecto-parasites that have a cosmopolitan distribution in tropical and subtropical regions of the world. Ticks cause economic losses in the form of reduced blood, meat and dairy products, as well as pathogen transmission. Different acaricides such as organochlorines, organophosphates, formamidines (e.g. amitraz), synthetic pyrethroids, macrocyclic lactones, fipronil, and fluazuron are currently used sequentially or simultaneously to control tick infestations. Most acaricide treatments now face increasingly high chances of failure, due to the resistance selection in different tick populations against these drugs. Acaricide resistance in ticks can be developed in different ways, including amino acid substitutions that result in morphological changes in the acaricide target, metabolic detoxification, and reduced acaricide entry through the outer layer of the tick body. The current literature brings a plethora of information regarding the use of different acaricides for tick control, resistance selection, analysis of mutations in target sites, and resistance mitigation. Alternatives such as synergistic use of different acaricides, plant-derived phytochemicals, fungi as biological control agents, and anti-tick vaccines have been recommended to avoid and mitigate acaricide resistance. The purpose of this review was to summarize and discuss different acaricides applied for tick control, their mechanisms of action and resistance selection, genetic polymorphisms in their target molecules, as well as the approaches used for diagnosis and mitigation of acaricide resistance, specifically in Rhipicephalus microplus ticks.
Host Immunization with Recombinant Tick Antigen and Evaluation of Host Immune Response. 国際誌査読有り

Kodai Kusakisako, Takeshi Miyata, Kozo Fujisaki, Tetsuya Tanaka

Methods in molecular biology (Clifton, N.J.)　2411　331-341　2022年

DOI： 10.1007/978-1-0716-1888-2_19 　

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Ticks are classified as hematophagous arthropods and transfer a variety of pathogens-such as viruses, bacteria, and protozoans-to vertebrate hosts during blood feeding. These transmitted pathogens cause infectious diseases that continue to affect both humans and animals worldwide. Chemical acaricides are commonly used for tick control to prevent infectious diseases. However, the continuous use of acaricides leads to the emergence of acaricide-resistant tick species; thus, alternative methods for tick control are necessary. Vaccination of vertebrate hosts with tick-derived molecules is considered to be a better alternative against ticks than chemical acaricides because ticks feed on host blood for several days and also concentrate the host blood with antibodies. On the other hand, the host's immune responses against pathogens mainly take two pathways-Th1 (cell-mediated immunity) and Th2 (humoral immunity) pathways. Thus, the vaccine can suggest which immune pathway is more important for vaccination. This chapter describes the procedures of immunizing laboratory animals-mice-with a recombinant tick protein for the preliminary evaluation of its potential as an anti-tick vaccine candidate. In addition, the method of evaluating the antigen-specific antibody production in the host using ELISA is described, as is the subsequent tick-infestation challenge for determining the effectiveness of vaccination.
Fundamental Tick Vaccinomic Approach to Evade Host Autoimmune Reaction. 国際誌査読有り

Seham H M Hendawy, Heba F Alzan, Tetsuya Tanaka, Mona S Mahmoud

Methods in molecular biology (Clifton, N.J.)　2411　343-358　2022年

DOI： 10.1007/978-1-0716-1888-2_20 　

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Ticks are obligate hematophagous ectoparasites that infect domestic animals, humans, and wildlife. Ticks can transmit a wide range of pathogens (viruses, rickettsia, bacteria, parasites, etc.), and some of those are of zoonotic importance. Tick-borne diseases have a negative economic impact in several tropical and subtropical countries. With climate change, tick distribution and tick-associated pathogens have increased. Currently, tick control procedures have more environmental drawbacks and there are pitfalls in vaccination process. Since vaccinations have helped to prevent several diseases and infections, several vaccination trials are ongoing to control ticks and tick-borne pathogens. However, autoimmune reactions to vaccinations are reported as an adverse reaction since vaccines were used to protect against disease in humans and animals. The antibodies against the vaccine antigen might harm similar antigen in the host. Therefore, in this chapter, we attempt to shed light on the importance of raising awareness of possible adverse events associated with vaccinations and the methods that should be used to address this problem. In silico and lab work should be performed ahead of the vaccination process to evaluate the vaccine candidates and avoid the vaccination opposing consequences.
Development of a loop-mediated isothermal amplification (LAMP) assay targeting the mitochondrial cytochrome b gene for the rapid detection of alveolar echinococcosis in hepatic nodules of horses. 国際誌査読有り

Tatsuro Hifumi, Kohei Akioka, Tetsuya Tanaka, Noriaki Miyoshi

Veterinary parasitology　299　109573-109573　2021年9月11日

DOI： 10.1016/j.vetpar.2021.109573 　

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Alveolar echinococcosis, which is caused by a larval-stage infection of Echinococcus multilocularis, is a zoonosis with public health importance. Recently, alveolar echinococcosis in slaughtered horses has been reported in Japan and Poland. In terms of public health, a highly sensitive and specific diagnostic method is essential for early detection during meat inspection. In this study, the loop-mediated isothermal amplification (LAMP) assay was developed and validated to target the mitochondrial cytochrome b (cob) gene of E. multilocularis. Forty-one hepatic solid nodules obtained from each horse were evaluated based on histopathological examination and cob-targeted PCR and then submitted to the LAMP assay. The optimal condition of the developed LAMP assay was 64℃ for 30 min. The results of the developed LAMP assay were completely consistent with those of cob PCR. In addition, the detection limit for the number of copies of the cob gene was 135 copies/μL in the LAMP assay. These findings suggest that the ability of the LAMP assay developed in this study is equivalent to that of the conventional PCR testing. The LAMP assay developed in this study can be used as an alternative to PCR testing for the routine genetic diagnosis of alveolar echinococcosis in horses.
Chromosome-Level Genome Sequence of Aspergillus puulaauensis MK2, a Fungus Isolated from a Dead Hard Tick. 国際誌査読有り

Taiki Futagami, Kazuki Mori, Chihiro Kadooka, Hiroko Niihara, Kosuke Tashiro, Hisanori Tamaki, Tetsuya Tanaka

Microbiology resource announcements　10　(36)　e0037221　2021年9月9日

DOI： 10.1128/MRA.00372-21 　

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Aspergillus puulaauensis strain MK2 was isolated from a dead hard tick (Haemaphysalis longicornis). Here, we determined the chromosome-level genome sequence of A. puulaauensis MK2.
Promoter analysis of elongation factor I-alpha(EFI α) derived from Haemaphysalis longicornis(和訳中)

Hernandez Emmanuel Pacia, 川田逸人, 山本大介, 加藤大智, 松林誠, 田仲哲也, 辻尚利, 八田岳士

日本寄生虫学会・日本臨床寄生虫学会合同大会プログラム・抄録集　90回・32回　58-58　2021年4月
出版者・発行元：日本寄生虫学会・日本臨床寄生虫学会
Relationship between hepatic grayish-white solid nodules in horses imported from Canada and larval Echinococcus multilocularis infection. 国際誌査読有り

Tatsuro Hifumi, Tetsuya Tanaka, Emmanuel Pacia Hernandez, Kohei Akioka, Kaori Yamada, Yasutaka Imamura, Hitoshi Hatai, Noriaki Miyoshi

The Canadian veterinary journal = La revue veterinaire canadienne　62　(3)　285-288　2021年3月

ISSN：0008-5286

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Histopathological and genetic examinations were conducted on grayish-white solid hepatic nodules in 150 horses imported from Canada, in order to investigate larval Echinococcus multilocularis infection. Ten of the 150 horses (6.7%) were diagnosed with alveolar hydatid disease. The sequences of the mitochondrial cytochrome b genes obtained from all 10 polymerase chain reaction positive samples had 99 to 100% identity with the European haplotype E1 of E. multilocularis. Therefore, we concluded that the infections likely originated in Canada.
Determination of resistance status to amitraz in the cattle tick Rhipicephalus (Boophilus) microplus from Luzon, Philippines, through bioassay and molecular analysis. 国際誌査読有り

Sherwin L Alota, Tisha Rogelle J Edquiban, Remil L Galay, John Michael G Bernardo, Kristina Andrea C Sandalo, Billy P Divina, Tetsuya Tanaka

Experimental & applied acarology　83　(3)　399-409　2021年2月16日

DOI： 10.1007/s10493-021-00593-8 　

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Tick infestations and tick-borne diseases are among the leading causes of economic losses in the cattle industry worldwide. Amitraz is one of the most commonly used chemical acaricides against the cattle tick Rhipicephalus (Boophilus) microplus. Resistance to amitraz has been reported in many countries but not in the Philippines. This study aimed to determine whether cattle ticks from provinces in northern and southern Luzon, Philippines, are resistant to amitraz. Engorged or nearly engorged female ticks were collected from 21 farms and allowed to lay eggs to produce larvae. Larval packet test (LPT) was performed using three concentrations of amitraz, as well as a negative control (diluent). Reverse-transcription polymerase chain reaction (RT-PCR) was also performed to amplify a fragment of the octopamine/tyramine receptor gene and was subjected to sequence analysis by multiple nucleotide and amino acid sequence alignments together with reference strain, amitraz-susceptible strain, and amitraz-resistant strain reported in other countries. LPT results suggest the absence of resistance in all of the tested populations. Interestingly, analysis of the octopamine/tyramine receptor amino acid sequence revealed four out of 14 larval pools having substitutions similar to that of the reported amitraz-resistant strains. Although no apparent resistance was observed in this study, prudent use of amitraz should be practiced as the development of resistance is still likely to occur in the future.
The antiviral immunity of ticks against transmitted viral pathogens. 国際誌査読有り

Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Takeshi Hatta, Kentaro Yoshii, Kodai Kusakisako, Naotoshi Tsuji, Tetsuya Tanaka

Developmental and comparative immunology　119　104012-104012　2021年1月21日

DOI： 10.1016/j.dci.2021.104012 　

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Ticks, being obligate hematophagous arthropods, are exposed to various blood-borne pathogens, including arboviruses. Consequently, their feeding behavior can readily transmit economically important viral pathogens to humans and animals. With this tightly knit vector and pathogen interaction, the replication and transmission of tick-borne viruses (TBVs) must be highly regulated by their respective tick vectors to avoid any adverse effect on the ticks' biological development and viability. Knowledge about the tick-virus interface, although gaining relevant advances in recent years, is advancing at a slower pace than the scientific developments related to mosquito-virus interactions. The unique and complicated feeding behavior of ticks, compared to that of other blood-feeding arthropods, also limits the studies that would further elaborate the antiviral immunity of ticks against TBVs. Hence, knowledge of molecular and cellular immune mechanisms at the tick-virus interface, will further elucidate the successful viral replication of TBVs in ticks and their effective transmission to human and animal hosts.
Molecular Prevalence of Anaplasma marginale and Ehrlichia in Domestic Large Ruminants and Rhipicephalus (Boophilus) microplus Ticks From Southern Luzon, Philippines. 国際誌査読有り

Remil L Galay, Carina R Llaneta, Maria Karla Faye B Monreal, Antero L Armero 3rd, Arianne Bel D Baluyut, Czarina Marie F Regino, Kristina Andrea C Sandalo, Billy P Divina, Melbourne R Talactac, Lennox P Tapawan, Maarten Czar L Mojares, Cherry R Alvarez, Emmanuel R Mago, Noemi D Encarnacion, Masako Andoh, Tetsuya Tanaka

Frontiers in veterinary science　8　746705-746705　2021年

DOI： 10.3389/fvets.2021.746705 　

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Anaplasmosis and ehrlichiosis are tick-borne rickettsial diseases that cause significant economic losses in the livestock industry worldwide. Although bovine anaplasmosis is known to be endemic in the Philippines, epidemiological data is fragmented. Moreover, little is known about bovine ehrlichiosis in the country. In this study, the prevalence of Anaplasma marginale and Ehrlichia in cattle and water buffalo from provinces in the southern part of Luzon, Philippines, was investigated through PCR. Blood samples from 620 animals comprised of 512 cattle and 108 water buffalo and 195 tick samples were subjected to nested PCR targeting the groESL gene of Anaplasmataceae. Positive samples were further subjected to another nested PCR and conventional PCR to amplify the A. marginale groEL gene and the Ehrlichia dsbA gene, respectively. Selected A. marginale-positive samples were also subjected to nested PCR targeting the msp5 gene. Regardless of the animal host, the overall prevalence in blood samples obtained was 51.9% for Anaplasmataceae, 43% for A. marginale, and 1.1% for Ehrlichia. No water buffalo were positive for Ehrlichia. Meanwhile, 15.9, 6.7, and 2% of the tick samples, all morphologically identified as Rhipicephalus (Boophilus) microplus, were positive for Anaplasmataceae, A. marginale, and Ehrlichia, respectively. Sequence analysis of selected A. marginale msp5 amplicons showed that the isolates from the region share 94-98% identity to reported A. marginale from other countries. The phylogenetic tree showed clustering of isolates in the region and a close relationship with A. marginale isolates from other countries. Sequences of Ehrlichia amplicons from cattle and ticks were 97-100% similar to reported Ehrlichia minasensis isolates. This study showed the high prevalence of A. marginale in Luzon, Philippines, and provided the first molecular evidence of E. minasensis in the country.
An Ixodes scapularis glutathione S-transferase plays a role in cell survival and viability during Langat virus infection of a tick cell line. 国際誌査読有り

Emmanuel Pacia Hernandez, Melbourne Rio Talactac, Rodel Jonathan Santos Vitor, Kentaro Yoshii, Tetsuya Tanaka

Acta tropica　214　105763-105763　2020年11月23日

DOI： 10.1016/j.actatropica.2020.105763 　

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Ticks are important vectors of diseases affecting both humans and animals. To be an efficient vector, ticks have to survive infection by pathogens such as the Langat virus (LGTV). One method utilized by ticks is their complex antioxidant mechanism. Included in the vast antioxidant processes are several enzymes involved in redox homeostasis. The ubiquitous glutathione S-transferases (GSTs) belong to the antioxidant family of enzymes. In this study, we evaluated the role of a GST during LGTV infection. ISE6 cells were infected with LGTV with a multiplicity of infection (MOI) of 0.01 and observed daily. The infection success was monitored via indirect immunofluorescent antibody test (IFAT) for LGTV for up to 4 days. The gene expression of IsGST1 was determined by real-time polymerase chain reaction (PCR) using IsGST1 gene-specific primers. Knockdown of the IsGST1 gene with subsequent LGTV infection was also performed. Afterward, ISE6 cell mortality and viability were checked daily until the fourth day. The virus titer from supernatants of IsGST1-knockdown cells was quantified using a focus-formation assay. IFAT data showed that LGTV infects ISE6 cells in a time-dependent manner with increasing infection from day 0 to day 4. The IsGST1 genes showed an increasing expression until day 2 of infection, while decreased expression was observed from day 3 to day 4 post-infection. Knockdown of the IsGST1 resulted in increased mortality on the third day of infection, while the cell viability was also negatively affected by the knockdown of the IsGST1 genes from day 0 to day 4 post-infection. Knockdown of the IsGST1 genes also resulted in a decreased viral titer from the supernatants of the ISE6 cells infected with LGTV. Based on the results, GSTs are possibly utilized both by cells and the virus for mutual survival and proliferation.
Development of a Loop-Mediated Isothermal Amplification (LAMP) Assay Targeting the Citrate Synthase Gene for Detection of Ehrlichia canis in Dogs 査読有り

Angela Patricia B. Chua, Remil L. Galay, Tetsuya Tanaka, Wataru Yamazaki

Veterinary Sciences　7　(4)　156-156　2020年10月15日
出版者・発行元： MDPI AG
DOI： 10.3390/vetsci7040156 　

eISSN：2306-7381

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Canine monocytic ehrlichiosis caused by Ehrlichia canis is one of the leading tick-borne diseases of dogs, particularly in tropical countries. A highly sensitive and specific diagnostic method is essential for early detection to facilitate treatment. This study was conducted to develop E. canis loop-mediated isothermal amplification (LAMP) assay, a highly sensitive yet simple molecular technique, targeting the citrate synthase (gltA) gene of E. canis. Canine blood samples were subjected to conventional PCR targeting E. canis gltA. After analysis of the sequences of PCR amplicons, LAMP primers were generated. The optimum temperature and time for the LAMP assay were determined using eight samples—after which, the effectiveness and reproducibility of LAMP were verified by testing 40 samples, which included PCR-positive and negative samples. The detection limit was also established. The optimal condition for the assay was 61 °C for 60 min. Compared to PCR, the LAMP assay had a relative sensitivity and specificity of 92.5 and 100%, respectively. Statistical analysis using McNemar’s test showed that the E. canis LAMP assay has no significant difference with PCR. Therefore, the LAMP assay developed in this study may be used as an alternative to PCR in the detection of E. canis.
ダニ研究の最前線とダニ媒介性感染症制御の可能性を探るマダニの吸血生理と抗マダニワクチン査読有り

田仲哲也

衛生動物　71　(2)　57-64　2020年6月
出版者・発行元：日本衛生動物学会
ISSN：0424-7086

eISSN：2185-5609
Molecular Detection of Rickettsia Spp. and Coxiella Burnetii in Cattle, Water Buffalo, and Rhipicephalus (Boophilus) Microplus Ticks in Luzon Island of the Philippines. 国際誌査読有り

Remil L Galay, Melbourne R Talactac, Bea V Ambita-Salem, Dawn Maureen M Chu, Lali Marie O Dela Costa, Cinnamon Mae A Salangsang, Darwin Kyle B Caracas, Florante H Generoso, Jonathan A Babelonia, Joeneil L Vergano, Lena C Berana, Kristina Andrea C Sandalo, Billy P Divina, Cherry R Alvarez, Emmanuel R Mago, Masako Andoh, Tetsuya Tanaka

Tropical medicine and infectious disease　5　(2)　2020年4月4日

DOI： 10.3390/tropicalmed5020054 　

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Rickettsia and Coxiella burnetii are zoonotic, tick-borne pathogens that can cause febrile illnesses with or without other symptoms in humans, but may cause subclinical infections in animals. There are only a few reports on the occurrence of these pathogens in cattle and water buffalo in Southeast Asia, including the Philippines. In this study, molecular detection of Rickettsia and C. burnetii in the blood and in the Rhipicephalus (Boophilus) microplus ticks of cattle and water buffalo from five provinces in Luzon Island of the Philippines was done. A total of 620 blood samples of cattle and water buffalo and 206 tick samples were collected and subjected to DNA extraction. After successful amplification of control genes, nested PCR was performed to detect gltA of Rickettsia and com1 of C. burnetii. No samples were positive for Rickettsia, while 10 (cattle = 7, water buffaloes = 3), or 1.6% of blood, and five, or 1.8% of tick samples, were C. burnetii-positive. Sequence analysis of the positive amplicons showed 99-100% similarity to reported C. burnetii isolates. This molecular evidence on the occurrence of C. burnetii in Philippine ruminants and cattle ticks and its zoonotic nature should prompt further investigation and surveillance to facilitate its effective control.
Expression analysis of glutathione S-transferases and ferritins during the embryogenesis of the tick Haemaphysalis longicornis. 国際誌査読有り

Emmanuel Pacia Hernandez, Kei Shimazaki, Hiroko Niihara, Rika Umemiya-Shirafuji, Kozo Fujisaki, Tetsuya Tanaka

Heliyon　6　(3)　e03644　2020年3月

DOI： 10.1016/j.heliyon.2020.e03644 　

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In the tick life cycle, embryogenesis is the only stage of development wherein no blood meal is required. Nevertheless, even in the absence of a blood meal, which is the source of nutrients as well as the ferrous iron and heme that could cause oxidative stress in ticks, malondialdehyde (MDA) has been reported to increase during this period. Additionally, the knockdown of some oxidative stress-related molecules such as ferritin has resulted in abnormal eggs and embryonic death. Here, we investigate the gene and protein expression profiles of the identified glutathione S-transferases (GSTs) and ferritins (Fers) of the tick H. longicornis during embryogenesis through quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blotting, respectively. We also confirm the lipid peroxidation and ferrous iron concentration level using a thiobarbituric acid reactive substances (TBARS) assay. Finally, we attempt to correlate these findings with the events occurring by establishing a staging process in H. longicornis embryos. Lipid peroxidation increased during the course of embryogenesis, as does the amount of GST proteins. On the other hand, the GST genes have high expression at the 1st day post-oviposition, during the early stage of embryogenesis and at day 10 during the period wherein the germ band is observable. Fer gene expression also starts to increase at day 10 and peaks at day 15. In the ferritin proteins, only the secretory ferritin (Fer2) is detected and constitutively expressed during embryogenesis. Events occurring during embryogenesis, such as energy production and iron metabolism for cellular proliferation and differentiation cause oxidative stress in the embryo. To counteract oxidative stress, it is possible that the embryo may utilize oxidative stress-related molecules such as GSTs and Fer2, which could be either maternally or embryo-derived.
A Peroxiredoxin From the Haemaphysalis longicornis Tick Affects Langat Virus Replication in a Hamster Cell Line. 国際誌査読有り

Kodai Kusakisako, Haruki Morokuma, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Kentaro Yoshii, Tetsuya Tanaka

Frontiers in cellular and infection microbiology　10　7-7　2020年

DOI： 10.3389/fcimb.2020.00007 　

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Ticks are hematophagous arthropods, and their blood feeding on vertebrate hosts is essential for their development. The vertebrate blood contains high levels of free iron that can react with oxygen in ticks, resulting in the production of hydrogen peroxide (H2O2), one of the reactive oxygen species. Peroxiredoxins (Prxs), H2O2-scavenging enzymes, take on an important role in the ticks' oxidative stress coping mechanism. Ticks also transmit several disease-causing pathogens, including tick-borne encephalitis virus (TBEV), in animals and humans. Therefore, the control of ticks and tick-borne pathogens is a key issue that needs to be addressed. Infection with an arthropod-borne flavivirus is known to induce oxidative stress in insect cells. We hypothesize that vector-derived Prxs could have an effect on the infection and/or replication of flaviviruses in the hosts, since ticks Prxs are possibly transmitted from ticks to their hosts. In this study, we established stable strains of baby hamster kidney (BHK) cells expressing two types of H2O2-scavenging Prxs from the hard tick Haemaphysalis longicornis (BHK-HlPrx and BHK-HlPrx2 cells). Although the infection of TBEV surrogate Langat virus (LGTV) did not induce H2O2 production in normal BHK cells, the mortality rate and the virus titer of LGTV infected BHK-HlPrx cells increased. In addition, HlPrx proteins in BHK cells can facilitate LGTV replication in cells, while HlPrx2 proteins in BHK cells cannot. The results also demonstrated that this facilitation of LGTV replication by the 1-Cys Prx in the BHK cells is not by scavenging H2O2 but by an unknown mechanism. In order to understand this mechanism, more studies using tick-derived cells and ticks are necessary.
The case for oxidative stress molecule involvement in the tick-pathogen interactions -an omics approach. 査読有り

Hernandez EP, Talactac MR, Fujisaki K, Tanaka T

Developmental and comparative immunology　100　103409　2019年11月

DOI： 10.1016/j.dci.2019.103409 　

ISSN：0145-305X
Characterization of an iron-inducible Haemaphysalis longicornis tick-derived promoter in an Ixodes scapularis-derived tick cell line (ISE6). 国際誌査読有り

Hernandez EP, Kusakisako K, Hatta T, Tanaka T

Parasites & vectors　12　(1)　321-321　2019年6月25日

DOI： 10.1186/s13071-019-3574-9 　

ISSN：1756-3305
Molecular detection of Anaplasma spp. In blood and milk of dairy cattle in the Philippines 査読有り

Angelito Pangan, Dela Cruz, Remil Linggatong Galay, Kristina Andrea, Claveria Sandalo, Flor Marie, Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka

Turkish Journal of Veterinary and Animal Sciences　43　540-545　2019年1月1日

DOI： 10.3906/vet-1903-27 　

ISSN：1300-0128
Molecular detection of tick-borne pathogens in canine population and Rhipicephalus sanguineus (sensu lato) ticks from southern Metro Manila and Laguna, Philippines. 査読有り

Galay RL, Manalo AAL, Dolores SLD, Aguilar IPM, Sandalo KAC, Cruz KB, Divina BP, Andoh M, Masatani T, Tanaka T

Parasites & vectors　11　(1)　643　2018年12月17日

DOI： 10.1186/s13071-018-3192-y 　

ISSN：1756-3305
A Continuing Exploration of Tick-Virus Interactions Using Various Experimental Viral Infections of Hard Ticks. 査読有り

Talactac MR, Hernandez EP, Fujisaki K, Tanaka T

Frontiers in physiology　9　1728　2018年12月4日

DOI： 10.3389/fphys.2018.01728 　

ISSN：1664-042X
Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6). 国際誌査読有り

Emmanuel Pacia Hernandez, Kodai Kusakisako, Melbourne Rio Talactac, Remil Linggatong Galay, Kentaro Yoshii, Tetsuya Tanaka

Scientific reports　8　(1)　16566-16566　2018年11月8日

DOI： 10.1038/s41598-018-34860-3 　

ISSN：2045-2322
Transcriptional activities of two newly identified Haemaphysalis longicornis tick-derived promoter regions in the Ixodes scapularis tick cell line (ISE6) 査読有り

Kusakisako K, Ido A, Masatani T, Morokuma H, Hernandez EP, Talactac MR, Yoshii K, Tanaka T

Insect Molecular Biology　27　(5)　590-602　2018年10月

DOI： 10.1111/imb.12497 　

ISSN：0962-1075
Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis. 国際誌査読有り

Hernandez EP, Kusakisako K, Talactac MR, Galay RL, Hatta T, Fujisaki K, Tsuji N, Tanaka T

Parasites & vectors　11　(1)　460-460　2018年8月9日

DOI： 10.1186/s13071-018-3044-9 　

ISSN：1756-3305
Hemolymph defensin from the hard tick Haemaphysalis longicornis attacks Gram-positive bacteria. 査読有り

Yada Y, Talactac MR, Kusakisako K, Hernandez EP, Galay RL, Andoh M, Fujisaki K, Tanaka T

Journal of invertebrate pathology　156　14-18　2018年7月

DOI： 10.1016/j.jip.2018.07.005 　

ISSN：0022-2011
The multiple roles of peroxiredoxins in tick blood feeding. 査読有り

Kusakisako K, Fujisaki K, Tanaka T

Experimental & applied acarology　75　(3)　269-280　2018年7月

DOI： 10.1007/s10493-018-0273-8 　

ISSN：0168-8162
Vector competence of Haemaphysalis longicornis ticks for a Japanese isolate of the Thogoto virus. 国際誌査読有り

Melbourne Rio Talactac, Kentaro Yoshii, Emmanuel Pacia Hernandez, Kodai Kusakisako, Remil Linggatong Galay, Kozo Fujisaki, Masami Mochizuki, Tetsuya Tanaka

Scientific reports　8　(1)　9300-9300　2018年6月18日

DOI： 10.1038/s41598-018-27483-1 　

ISSN：2045-2322
Peroxiredoxins are important for the regulation of hydrogen peroxide concentrations in ticks and tick cell line 査読有り

Kodai Kusakisako, Emmanuel Pacia Hernandez, Melbourne Rio Talactac, Kentaro Yoshii, Rika Umemiya-Shirafuji, Kozo Fujisaki, Tetsuya Tanaka

Ticks and Tick-borne Diseases　9　(4)　872-881　2018年5月

DOI： 10.1016/j.ttbdis.2018.03.016 　

ISSN：1877-959X
Immunofluorescent detection in the ovary of host antibodies against a secretory ferritin injected into female Haemaphysalis longicornis ticks 査読有り

Remil Linggatong Galay, Tomohide Matsuo, Emmanuel Pacia Hernandez, Melbourne Rio Talactac, Kodai Kusakisako, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

Parasitology International　67　(2)　119-122　2018年4月1日

DOI： 10.1016/j.parint.2017.10.006 　

ISSN：1873-0329 1383-5769
Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feeding 査読有り

Emmanuel Pacia Hernandez, Kodai Kusakisako, Melbourne Rio Talactac, Remil Linggatong Galay, Takeshi Hatta, Tomohide Matsuo, Kozo Fujisaki, Naotoshi Tsuji, Tetsuya Tanaka

Parasites and Vectors　11　(1)　91　2018年2月8日

DOI： 10.1186/s13071-018-2667-1 　

ISSN：1756-3305
Evaluation of vaccine potential of 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis. 国際誌査読有り

Kodai Kusakisako, Takeshi Miyata, Masashi Tsujio, Remil Linggatong Galay, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Kozo Fujisaki, Tetsuya Tanaka

Experimental & applied acarology　74　(1)　73-84　2018年1月

DOI： 10.1007/s10493-018-0209-3 　

ISSN：0168-8162
マダニペルオキシレドキシンの役割特に吸血における抗酸化機構と宿主の免疫応答について

草木迫浩大, 藤崎幸藏, 田仲哲也

獣医寄生虫学会誌　16　(1-2)　22-28　2017年12月
出版者・発行元：日本獣医寄生虫学会
ISSN：1347-961X
Topics from special edition マダニの鉄代謝を制御するフェリチンの展望

田仲哲也, ガライレミル, リングガートン, 白藤(梅宮) 梨可, 藤﨑幸蔵

細胞　49　(14)　715-719　2017年12月
出版者・発行元：ニューサイエンス社
ISSN：1346-7557
フタトゲチマダニヘモリンフ由来ディフェンシンの同定とその抗菌作用

屋田友里花, 草木迫浩大, Talactac Melbourne R., Hernandez Emmanuel P., 前田大輝, 安藤匡子, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　68　(2)　75-75　2017年7月
出版者・発行元：日本衛生動物学会
ISSN：0424-7086

eISSN：2185-5609
Synchronous Langat Virus Infection of Haemaphysalis longicornis Using Anal Pore Microinjection 査読有り

Melbourne Rio Talactac, Kentaro Yoshii, Emmanuel Pacia Hernandez, Kodai Kusakisako, Remil Linggatong Galay, Kozo Fujisaki, Masami Mochizuki, Tetsuya Tanaka

VIRUSES-BASEL　9　(7)　2017年7月

DOI： 10.3390/v9070189 　

ISSN：1999-4915
Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis 査読有り

Melbourne Rio Talactac, Yurika Yada, Kentaro Yoshii, Emmanuel Pacia Hernandez, Kodai Kusakisako, Hiroki Maeda, Remil Linggatong Galay, Kozo Fujisaki, Masami Mochizuki, Tetsuya Tanaka

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY　68　98-107　2017年3月

DOI： 10.1016/j.dci.2016.11.013 　

ISSN：0145-305X

eISSN：1879-0089
Initial development of Babesia ovata in the tick midgut 査読有り

Hiroki Maeda, Takeshi Hatta, M. Abdul Alim, Daigo Tsubokawa, Fusako Mikami, Kodai Kusakisako, Makoto Matsubayashi, Rika Umemiya-Shirafuji, Naotoshi Tsuji, Tetsuya Tanaka

VETERINARY PARASITOLOGY　233　39-42　2017年1月

DOI： 10.1016/j.vetpar.2016.11.020 　

ISSN：0304-4017

eISSN：1873-2550
Detection and molecular characterization of Babesia, Theileria, and Hepatozoon species in hard ticks collected from Kagoshima, the southern region in Japan 査読有り

Tatsunori Masatani, Kei Hayashi, Masako Andoh, Morihiro Tateno, Yasuyuki Endo, Masahito Asada, Kodai Kusakisako, Tetsuya Tanaka, Mutsuyo Gokuden, Nodoka Hozumi, Fumiko Nakadohzono, Tomohide Matsuo

TICKS AND TICK-BORNE DISEASES　8　(4)　581-587　2017年

DOI： 10.1016/j.ttbdis.2017.03.007 　

ISSN：1877-959X

eISSN：1877-9603
Establishment of a novel tick-Babesia experimental infection model 査読有り

Hiroki Maeda, Takeshi Hatta, M. Abdul Alim, Daigo Tsubokawa, Fusako Mikami, Makoto Matsubayashi, Takeharu Miyoshi, Rika Umemiya-Shirafuji, Shin-ichiro Kawazu, Ikuo Igarashi, Masami Mochizuki, Naotoshi Tsuji, Tetsuya Tanaka

SCIENTIFIC REPORTS　6　37039　2016年11月

DOI： 10.1038/srep37039 　

ISSN：2045-2322
Improvement of the cryopreservation method for the Babesia gibsoni parasite by using commercial freezing media 査読有り

Kodai Kusakisako, Tatsunori Masatani, Yurika Yada, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Hiroki Maeda, Masami Mochizuki, Tetsuya Tanaka

PARASITOLOGY INTERNATIONAL　65　(5)　532-535　2016年10月

DOI： 10.1016/j.parint.2016.02.012 　

ISSN：1383-5769
2-Cys peroxiredoxin is required in successful blood-feeding, reproduction, and antioxidant response in the hard tick Haemaphysalis longicornis 査読有り

Kodai Kusakisako, Remil Linggatong Galay, Rika Umemiya-Shirafuji, Emmanuel Pacia Hernandez, Hiroki Maeda, Melbourne Rio Talactac, Naotoshi Tsuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PARASITES & VECTORS　9　457　2016年8月

DOI： 10.1186/s13071-016-1748-2 　

ISSN：1756-3305
Impaired cellular immune response to injected bacteria after knockdown of ferritin genes in the hard tick Haemaphysalis longicornis 査読有り

Remil Linggatong Galay, Rie Takechi, Rika Umemiya-Shirafuji, Melbourne Rio Talactac, Hiroki Maeda, Kodai Kusakisako, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PARASITOLOGY INTERNATIONAL　65　(3)　251-257　2016年6月

DOI： 10.1016/j.parint.2016.01.007 　

ISSN：1383-5769
鹿児島県屋久島のヒゲナガチマダニから分離されたリケッチアHKT-1株の遺伝子解析

安藤匡子, 本田俊郎, 御供田睦代, 藤田博己, 矢野泰弘, 正谷達謄, 田仲哲也, 松尾智英, 遠藤泰之, 安藤秀二, 川端寛樹

衛生動物　67　(Suppl.)　91-91　2016年4月
出版者・発行元：日本衛生動物学会
ISSN：0424-7086

eISSN：2185-5609
A novel C-type lectin with triple carbohydrate recognition domains has critical roles for the hard tick Haemaphysalis longicornis against Gram-negative bacteria 査読有り

Hiroki Maeda, Takeshi Miyata, Kodai Kusakisako, Remil Linggatong Galay, Melbourne Rio Talactac, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY　57　38-47　2016年4月

DOI： 10.1016/j.dci.2015.12.015 　

ISSN：0145-305X

eISSN：1879-0089
Dynamics of Theileria orientalis genotype population in cattle in a year-round grazing system 査読有り

Tatsunori Masatani, Shunpei Yoshihara, Atsuko Matsubara, Takafumi Gotoh, Hideyuki Takahashi, Tetsuya Tanaka, Masako Andoh, Yasuyuki Endo, Tomohide Matsuo

ACTA PARASITOLOGICA　61　(2)　419-424　2016年3月

DOI： 10.1515/ap-2016-0056 　

ISSN：1230-2821

eISSN：1896-1851
Functional analysis of recombinant 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis 査読有り

K. Kusakisako, T. Masatani, T. Miyata, R. L. Galay, H. Maeda, M. R. Talactac, N. Tsuji, M. Mochizuki, K. Fujisaki, T. Tanaka

INSECT MOLECULAR BIOLOGY　25　(1)　16-23　2016年2月

DOI： 10.1111/imb.12193 　

ISSN：0962-1075

eISSN：1365-2583
Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus 査読有り

Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami Mochizuki

PARASITES & VECTORS　9　59　2016年2月

DOI： 10.1186/s13071-016-1344-5 　

ISSN：1756-3305
Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice 査読有り

Yuina Ono, Makoto Matsubayashi, Hiroaki Kawaguchi, Masashi Tsujio, Masanobu Mizuno, Tetsuya Tanaka, Tatsunori Masatani, Toshihiro Matsui, Tomohide Matsuo

PARASITOLOGY RESEARCH　115　(1)　211-215　2016年1月

DOI： 10.1007/s00436-015-4737-z 　

ISSN：0932-0113

eISSN：1432-1955
Role of the tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) from the hard tick Haemaphysalis longicornis in immunity against bacterial infection 査読有り

Rie Takechi, Remil Linggatong Galay, Tomohide Matsuo, Hiroki Maeda, Kodai Kusakisako, Melbourne Rio Talactac, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

TICKS AND TICK-BORNE DISEASES　7　(1)　36-45　2016年

DOI： 10.1016/j.ttbdis.2015.08.002 　

ISSN：1877-959X

eISSN：1877-9603
Induction of gene silencing in Haemaphysalis longicornis ticks through immersion in double-stranded RNA 査読有り

Remil Linggatong Galay, Emmanuel Pacia Hernandez, Melbourne Rio Talactac, Hiroki Maeda, Kodai Kusakisako, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

TICKS AND TICK-BORNE DISEASES　7　(5)　813-816　2016年

DOI： 10.1016/j.ttbdis.2016.03.018 　

ISSN：1877-959X

eISSN：1877-9603
Host Immunization with Recombinant Proteins to Screen Antigens for Tick Control. 国際誌査読有り

Galay RL, Miyata T, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T

Methods in molecular biology (Clifton, N.J.)　1404　261-273　2016年

DOI： 10.1007/978-1-4939-3389-1_18 　

ISSN：1064-3745
The vector potential of Haemaphysalis longicornis ticks for Babesia microti parasites under experimental condition 査読有り

K. Kusakisako, H. Maeda, R. L. Galay, T. Matsuo, M. Tsujio, R. Umemiya-Shirafuji, M. Mochizuki, K. Fujisaki, T. Tanaka

JOURNAL OF PROTOZOOLOGY RESEARCH　25　(1-2)　8-17　2015年12月

ISSN：0917-4427
Evaluation of Eimeria krijgsmanni as a murine model for testing the efficacy of anti-parasitic agents 査読有り

Toshinori Takeo, Tetsuya Tanaka, Makoto Matsubayashi, Masashi Tsujio, Rika Umemiya-Shirafuji, Naotoshi Tsuji, Kozo Fujisaki, Toshihiro Matsui, Tomohide Matsuo

ACTA PARASITOLOGICA　60　(2)　190-195　2015年6月

DOI： 10.1515/ap-2015-0027 　

ISSN：1230-2821

eISSN：1896-1851
Identification of the Babesia-responsive leucine-rich repeat domain-containing protein from the hard tick Haemaphysalis longicornis 査読有り

Hiroki Maeda, Koshi Kurisu, Takeshi Miyata, Kodai Kusakisako, Remil Linggatong Galay, Talactac Melbourne Rio, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PARASITOLOGY RESEARCH　114　(5)　1793-1802　2015年5月

DOI： 10.1007/s00436-015-4365-7 　

ISSN：0932-0113

eISSN：1432-1955
Iron metabolism in hard ticks (Acari: Ixodidae): The antidote to their toxic diet 査読有り

Remil Linggatong Galay, Rika Umemiya-Shirafuji, Masami Mochizuld, Kozo Fujisald, Tetsuya Tanaka

PARASITOLOGY INTERNATIONAL　64　(2)　182-189　2015年4月

DOI： 10.1016/j.parint.2014.12.005 　

ISSN：1383-5769
The widely distributed hard tick, Haemaphysalis longicornis, can retain canine parvovirus, but not be infected in laboratory condition 査読有り

Hiroyuki Mori, Tetsuya Tanaka, Masami Mochizuki

JOURNAL OF VETERINARY MEDICAL SCIENCE　77　(4)　405-411　2015年4月

DOI： 10.1292/jvms.14-0199 　

ISSN：0916-7250

eISSN：1347-7439
Evaluation and comparison of the potential of two ferritins as anti-tick vaccines against Haemaphysalis longicornis 査読有り

Remil Linggatong Galay, Takeshi Miyata, Rika Umemiya-Shirafuji, Hiroki Maeda, Kodai Kusakisako, Naotoshi Tsuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PARASITES & VECTORS　7　482　2014年10月

DOI： 10.1186/s13071-014-0482-x 　

ISSN：1756-3305
Molecular and phylogenetic characterizations of an Eimeria krijgsmanni Yakimoff & Gouseff, 1938 (Apicomplexa: Eimeriidae) mouse intestinal protozoan parasite by partial 18S ribosomal RNA gene sequence analysis 査読有り

Toshinori Takeo, Tetsuya Tanaka, Makoto Matsubayashi, Hiroki Maeda, Kodai Kusakisako, Toshihiro Matsui, Masami Mochizuki, Tomohide Matsuo

PARASITOLOGY INTERNATIONAL　63　(4)　627-630　2014年8月

DOI： 10.1016/j.parint.2014.04.004 　

ISSN：1383-5769
Host specificity and in vivo infectivities of the mouse coccidian parasites Eimeria krijgsmanni 査読有り

Kazuki Hashimoto, Tetsuya Tanaka, Makoto Matsubayashi, Kyoko Endo, Rika Umemiya-Shirafuji, Toshihiro Matsui, Tomohide Matsuo

ACTA PARASITOLOGICA　59　(2)　337-342　2014年6月

DOI： 10.2478/s11686-014-0251-1 　

ISSN：1230-2821

eISSN：1896-1851
Expression analysis of autophagy-related genes in the hard tick Haemaphysalis longicornis 査読有り

Rika Umemiya-Shirafuji, Remil Linggatong Galay, Hiroki Maeda, Suguru Kawano, Tetsuya Tanaka, Shinya Fukumoto, Hiroshi Suzuki, Naotoshi Tsuji, Kozo Fujisaki

VETERINARY PARASITOLOGY　201　(1-2)　169-175　2014年3月

DOI： 10.1016/j.vetpar.2014.01.024 　

ISSN：0304-4017

eISSN：1873-2550
Two Kinds of Ferritin Protect Ixodid Ticks from Iron Overload and Consequent Oxidative Stress 査読有り

Remil Linggatong Galay, Rika Umemiya-Shirafuji, Eugene T. Bacolod, Hiroki Maeda, Kodai Kusakisako, Jiro Koyama, Naotoshi Tsuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PLOS ONE　9　(3)　e90661　2014年3月

DOI： 10.1371/journal.pone.0090661 　

ISSN：1932-6203
Host-derived transferrin is maintained and transferred from midgut to ovary in Haemaphysalis longicomis ticks 査読有り

Hiroyuki Mori, Remil Linggatong Galay, Hiroki Maeda, Tomohide Matsuo, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

TICKS AND TICK-BORNE DISEASES　5　(2)　121-126　2014年

DOI： 10.1016/j.ttbdis.2013.09.004 　

ISSN：1877-959X

eISSN：1877-9603
TgGRA23, a novel Toxoplasma gondii dense granule protein associated with the parasitophorous vacuole membrane and intravacuolar network 査読有り

Tatsunori Masatani, Tomohide Matsuo, Tetsuya Tanaka, Mohamad Alaa Terkawi, Eung-Goo Lee, Youn-Kyoung Goo, Gabriel Oluga Aboge, Junya Yamagishi, Kei Hayashi, Kyohko Kameyama, Shinuo Cao, Yoshifumi Nishikawa, Xuenan Xuan

PARASITOLOGY INTERNATIONAL　62　(4)　372-379　2013年8月

DOI： 10.1016/j.parint.2013.04.003 　

ISSN：1383-5769
Inhibitory effect of cyclophilin A from the hard tick Haemaphysalis longicornis on the growth of Babesia bovis and Babesia bigemina 査読有り

Hiroki Maeda, Damdinsuren Boldbaatar, Kodai Kusakisako, Remil Linggatong Galay, Kyaw Min Aung, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PARASITOLOGY RESEARCH　112　(6)　2207-2213　2013年6月

DOI： 10.1007/s00436-013-3390-7 　

ISSN：0932-0113
Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis 査読有り

Remil Linggatong Galay, Kyaw Min Aung, Rika Umemiya-Shirafuji, Hiroki Maeda, Tomohide Matsuo, Hiroaki Kawaguchi, Noriaki Miyoshi, Hiroshi Suzuki, Xuenan Xuan, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

JOURNAL OF EXPERIMENTAL BIOLOGY　216　(10)　1905-1915　2013年5月

DOI： 10.1242/jeb.081240 　

ISSN：0022-0949
Target of rapamycin (TOR) controls vitellogenesis via activation of the S6 kinase in the fat body of the tick, Haemaphysalis longicornis 査読有り

Rika Umemiya-Shirafuji, Damdinsuren Boldbaatar, Min Liao, Banzragch Battur, Md. Morshedur Rahman, Thasaneeya Kuboki, Remit Linggatong Galay, Tetsuya Tanaka, Kozo Fujisaki

INTERNATIONAL JOURNAL FOR PARASITOLOGY　42　(11)　991-998　2012年10月

DOI： 10.1016/j.ijpara.2012.08.002 　

ISSN：0020-7519

eISSN：1879-0135
Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice 査読有り

Longzheng Yu, Junya Yamagishi, Shoufa Zhang, Chunmei Jin, Gabriel Oluga Aboge, Houshuang Zhang, Guohong Zhang, Tetsuya Tanaka, Kozo Fujisaki, Yoshifumi Nishikawa, Xuenan Xuan

PARASITOLOGY INTERNATIONAL　61　(3)　481-486　2012年9月

DOI： 10.1016/j.parint.2012.04.001 　

ISSN：1383-5769
RNAi of the translation inhibition gene 4E-BP identified from the hard tick, Haemaphysalis longicornis, affects lipid storage during the off-host starvation period of ticks 査読有り

Aiko Kume, Damdinsuren Boldbaatar, Yuko Takazawa, Rika Umemiya-Shirafuji, Tetsuya Tanaka, Kozo Fujisaki

PARASITOLOGY RESEARCH　111　(2)　889-896　2012年8月

DOI： 10.1007/s00436-012-2915-9 　

ISSN：0932-0113
Parasiticidal activity of Haemaphysalis longicornis longicin P4 peptide against Toxoplasma gondii 査読有り

Tetsuya Tanaka, Hiroki Maeda, Tomohide Matsuo, Damdinsuren Boldbattar, Rika Umemiya-Shirafuji, Aiko Kume, Hiroshi Suzuki, Xuenan Xuan, Naotoshi Tsuji, Kozo Fujisaki

PEPTIDES　34　(1)　242-250　2012年3月

DOI： 10.1016/j.peptides.2011.07.027 　

ISSN：0196-9781
Akt is an essential player in regulating cell/organ growth at the adult stage in the hard tick Haemaphysalis longicornis 査読有り

Rika Umemiya-Shirafuji, Toru Tanaka, Damdinsuren Boldbaatar, Tetsuya Tanaka, Kozo Fujisaki

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY　42　(3)　164-173　2012年3月

DOI： 10.1016/j.ibmb.2011.12.001 　

ISSN：0965-1748
HlSRB, a Class B Scavenger Receptor, Is Key to the Granulocyte-Mediated Microbial Phagocytosis in Ticks 査読有り

Kyaw Min Aung, Damdinsuren Boldbaatar, Rika Umemiya-Shirafuji, Min Liao, Naotoshi Tsuji, Xuan Xuenan, Hiroshi Suzuki, Aiko Kume, Remil Linggatong Galay, Tetsuya Tanaka, Kozo Fujisaki

PLOS ONE　7　(3)　e33504　2012年3月

DOI： 10.1371/journal.pone.0033504 　

ISSN：1932-6203
Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaphysalis longicornis 査読有り

Remil Linggatong Galay, Hiroki Maeda, Kyaw Min Aung, Rika Umemiya-Shirafuji, Xuenan Xuan, Ikuo Igarashi, Naotoshi Tsuji, Tetsuya Tanaka, Kozo Fujisaki

TROPICAL ANIMAL HEALTH AND PRODUCTION　44　(2)　343-348　2012年2月

DOI： 10.1007/s11250-011-0027-7 　

ISSN：0049-4747
Tick longicin implicated in the arthropod transmission of toxoplasma gondii 査読有り

Tetsuya Tanaka, Hiroki Maeda, Remil Linggatong Galay, Damdinsuren Boldbattar, Rika Umemiya-Shirafuji, Hiroshi Suzuki, Xuenan Xuan, Naotoshi Tsuji, Kozo Fujisaki

Journal of Veterinary Science and Technology　3　(2)　2012年

DOI： 10.4172/2157-7579.1000112 　

ISSN：2157-7579
Scavenger Receptor Mediates Systemic RNA Interference in Ticks 査読有り

Kyaw Min Aung, Damdinsuren Boldbaatar, Rika Umemiya-Shirafuji, Min Liao, Xuan Xuenan, Hiroshi Suzuki, Remil Linggatong Galay, Tetsuya Tanaka, Kozo Fujisaki

PLOS ONE　6　(12)　e28407　2011年12月

DOI： 10.1371/journal.pone.0028407 　

ISSN：1932-6203
Cloning and characterization of the autophagy-related gene 6 from the hard tick, Haemaphysalis longicornis 査読有り

Suguru Kawano, Rika Umemiya-Shirafuji, Damdinsuren Boldbaatar, Kenji Matsuoka, Tetsuya Tanaka, Kozo Fujisaki

PARASITOLOGY RESEARCH　109　(5)　1341-1349　2011年11月

DOI： 10.1007/s00436-011-2429-x 　

ISSN：0932-0113
Effects of Daily Oral Administration of Difructose Anhydride III on Health Status, Blood Parameters and Faecal Shedding of Coliform Bacteria of Japanese Black Calves during the Pre-weaning Period 査読有り

M. Takagi, H. Hasunuma, D. Matsumoto, T. Obi, K. Takase, M. Ohtani, T. Sato, U. Watanabe, K. Okamoto, T. Tanaka, C. Tshering, E. Deguchi

ANIMAL NUTRITION AND FEED TECHNOLOGY　11　(2)　147-158　2011年7月

ISSN：0972-2963
ウシラクトペルオキシダーゼのin vitroでの細胞傷害防止効果について

石川稿大郎, 田仲哲也, 島崎敬一

ミルクサイエンス　60　(1)　1-6　2011年4月
出版者・発行元：日本酪農科学会
ISSN：1343-0289
Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis 査読有り

Kyaw Min Aung, Damdinsuren Boldbaatar, Min Liao, Rika Umemiya-Shirafuji, Sumihiro Nakao, Terushige Matsuoka, Tetsuya Tanaka, Kozo Fujisaki

PARASITOLOGY RESEARCH　108　(2)　273-285　2011年2月

DOI： 10.1007/s00436-010-2053-1 　

ISSN：0932-0113
Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development 査読有り

Damdinsuren Boldbaatar, Rika Umemiya-Shirafuji, Min Liao, Tetsuya Tanaka, Xuenan Xuan, Kozo Fujisaki

JOURNAL OF INSECT PHYSIOLOGY　56　(11)　1587-1598　2010年11月

DOI： 10.1016/j.jinsphys.2010.05.019 　

ISSN：0022-1910

eISSN：1879-1611
Parasiticidal activity of human alpha-defensin-5 against Toxoplasma gondii 査読有り

Tetsuya Tanaka, Md Morshedur Rahman, Banzragch Battur, Damdinsuren Boldbaatar, Min Liao, Rika Umemiya-Shirafuji, Xuenan Xuan, Kozo Fujisaki

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL　46　(6)　560-565　2010年6月

DOI： 10.1007/s11626-009-9271-9 　

ISSN：1071-2690

eISSN：1543-706X
Structural Characterization and Cytolytic Activity of a Potent Antimicrobial Motif in Longicin, a Defensin-Like Peptide in the Tick Haemaphysalis longicornis 査読有り

Md. Morshedur Rahman, Naotoshi Tsuji, Damdinsuren Boldbaatar, Banzragch Battur, Min Liao, Rika Umemiya-Shirafuji, Myungjo You, Tetsuya Tanaka, Kozo Fujisaki

JOURNAL OF VETERINARY MEDICAL SCIENCE　72　(2)　149-156　2010年2月

DOI： 10.1292/jvms.09-0167 　

ISSN：0916-7250
GATA transcription, translation and regulation in Haemaphysalis longicornis tick: Analysis of the cDNA and an essential role for vitellogenesis 査読有り

Damdinsuren Boldbaatar, Banzragch Battur, Rika Umemiya-Shirafuji, Min Liao, Tetsuya Tanaka, Kozo Fujisaki

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY　40　(1)　49-57　2010年1月

DOI： 10.1016/j.ibmb.2009.12.009 　

ISSN：0965-1748

eISSN：1879-0240
The identification and characterization of lysozyme from the hard tick Haemaphysalis longicornis 査読有り

Tetsuya Tanaka, Suguru Kawano, Sumihiro Nakao, Rika Umemiya-Shirafuji, Md. Morshedur Rahman, Damdinsuren Boldbaatar, Banzragch Battur, Min Liao, Kozo Fujisaki

TICKS AND TICK-BORNE DISEASES　1　(4)　178-185　2010年

DOI： 10.1016/j.ttbdis.2010.09.001 　

ISSN：1877-959X

eISSN：1877-9603
Effects of Oral Administration of Difructose Anhydride III on Selected Health and Blood Parameters of Group-housed Japanese Black Calves during the Preweaning Period 査読有り

Daisaku Matsumoto, Mitsuhiro Takagi, Hiroshi Hasunuma, Yasuo Fushimi, Masayuki Ohtani, Tadashi Sato, Koji Okamoto, Francis Shahada, Tetsuya Tanaka, Eisaburo Deguchi

ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　22　(12)　1640-1647　2009年12月

DOI： 10.5713/ajas.2009.90199 　

ISSN：1011-2367

eISSN：1976-5517
Activation mechanism of recombinant Der p 3 allergen zymogen - Contribution of cysteine protease Der p 1 and effect of propeptide glycosylation 査読有り

Marie-Eve Dumez, Nathalie Teller, Frederic Mercier, Tetsuya Tanaka, Isabel Vandenberghe, Michel Vandenbranden, Bart Devreese, Andre Luxen, Jean-Marie Frere, Andre Matagne, Alain Jacquet, Moreno Galleni, Andy Chevigne

JOURNAL OF BIOLOGICAL CHEMISTRY　283　(45)　30606-30617　2008年11月

DOI： 10.1074/jbc.M803041200 　

ISSN：0021-9258
Expression and characterization of bovine lactoperoxidase by recombinant vaccinia virus 査読有り

Tetsuya Tanaka, Xuenan Xuan, Asato Kojima, Ikuo Igarashi, Kozo Fujisaki, Kei-ichi Shimazaki

CYTOTECHNOLOGY　58　(3)　127-133　2008年11月

DOI： 10.1007/s10616-009-9181-4 　

ISSN：0920-9069
Identification of two forms of cyclophilin from the hard tick Haemaphysalis longicornis 査読有り

Damdinsuren Boldbaatar, Robert Musyoka Kilonzo, Banzragch Battur, Rika Umemiya, Min Liao, Tetsuya Tanaka, Xuenan Xuan, Kozo Fujisaki

PROCESS BIOCHEMISTRY　43　(6)　615-625　2008年6月

DOI： 10.1016/j.procbio.2008.01.026 　

ISSN：1359-5113
DiC14-amidine cationic liposomes stimulate myeloid dendritic cells through Toll-like receptor 4 査読有り

Tetsuya Tanaka, Amandine Legat, Emmanuelle Adam, Jonathan Steuve, Jean-Stephane Gatot, Michel Vandenbranden, Liliana Ulianov, Caroline Lonez, Jean-Marie Ruysschaert, Eric Muraille, Marcel Tuynder, Michel Goldman, Alain Jacquet

EUROPEAN JOURNAL OF IMMUNOLOGY　38　(5)　1351-1357　2008年5月

DOI： 10.1002/eji.200737998 　

ISSN：0014-2980
Functional analysis of protein disulfide isomerases in blood feeding, viability and oocyte development in Haemaphysalis longicornis ticks 査読有り

Min Liao, Daindinsuren Boldbaatar, Haiyan Gong, Penglong Huang, Rika Umemiya, Thasaneeya Harnnoi, Jinlin Zhou, Tetsuya Tanaka, Hiroshi Suzuki, Xuenan Xuan, Kozo Fujisaki

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY　38　(3)　285-295　2008年3月

DOI： 10.1016/j.ibmb.2007.11.006 　

ISSN：0965-1748
Profiles of bovine lactoferrin in the gastrointestinal tracts of rats as observed by ELISA, Western blotting and SELDI-affinity MS 査読有り

Ran E. Yoshise, M. Matsumoto, H. Chiji, H. Kuwata, K. Shin, K. Yamauchi, Y. Tamura, T. Tanaka, H. Kumura, K. Shimazaki

MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL　62　(4)　446-450　2007年

ISSN：0026-3788
Reactivity of synthetic SAG1 (p30) peptide sequences with RH, S273 and beverley strain-induced anti-Toxoplasma gondii antibodies 査読有り

M. Kato, F. G. Claveria, Y. Maki, K. Sanda, T. Tanaka, Y. Omata, H. Nagasawa, N. Suzuki

PATHOBIOLOGY　74　(1)　50-56　2007年

DOI： 10.1159/000101051 　

ISSN：1015-2008
Parasiticidal activity of bovine lactoperoxidase against Toxoplasma gondii 査読有り

Tetsuya Tanaka, Shin Murakami, Haruto Kumura, Ikuo Igarashi, Kei-ichi Shimazaki

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE　84　(5)　774-779　2006年10月

DOI： 10.1139/O06-142 　

ISSN：0829-8211
Parasiticidal activity of bovine lactoperoxidase against Toxoplasma gondii

Tetsuya Tanaka, Shin Murakami, Haruto Kumura, Ikuo Igarashi, Kei-ichi Shimazaki

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE　84　(5)　774-779　2006年10月

DOI： 10.1139/O06-142 　

ISSN：0829-8211
Short report: Inhibitory effect of lactoferrin on in vitro growth of Babesia caballi 査読有り

H Ikadai, T Tanaka, N Shibahara, H Tanaka, A Matsuu, N Kudo, K Shimazaki, Igarashi, I, T Oyamada

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE　73　(4)　710-712　2005年10月

ISSN：0002-9637
Toxoplasma gondii antigens GRA1 (p24) and SAG1 (p30): A comparison of their stimulatory influence on T-cell activation and cytokine expression in in vitro cultures

M Kato, FG Claveria, Y Maki, T Tanaka, N Suzuki, H Nagasawa

PATHOBIOLOGY　72　(3)　160-164　2005年

DOI： 10.1159/000084120 　

ISSN：1015-2008

eISSN：1423-0291
Susceptibility of bovine osteopontin to chymosin 査読有り

H Kumura, A Miura, E Sato, T Tanaka, K Shimazaki

JOURNAL OF DAIRY RESEARCH　71　(4)　500-504　2004年11月

DOI： 10.1017/S0022029904000391 　

ISSN：0022-0299
Susceptibilities against bovine lactoferrin with microorganisms isolated from mastitic milk 査読有り

NY Lee, K Kawai, Nakamura, I, T Tanaka, H Kumura, K Shimazaki

JOURNAL OF VETERINARY MEDICAL SCIENCE　66　(10)　1267-1269　2004年10月

DOI： 10.1292/jvms.66.1267 　

ISSN：0916-7250
Detection of bovine lactoferrin binding protein on Jurkat human lymphoblastic T cell line 査読有り

T Tanaka, H Morita, YC Yoo, WS Kim, H Kumura, KI Shimazaki

JOURNAL OF VETERINARY MEDICAL SCIENCE　66　(7)　865-869　2004年7月

DOI： 10.1292/jvms.66.865 　

ISSN：0916-7250
Growth-promoting effects of lactoferrin on L-acidophilus and Bifidobacterium spp. 査読有り

WS Kim, M Ohashi, T Tanaka, H Kumura, GY Kim, IK Kwon, JS Goh, K Shimazaki

BIOMETALS　17　(3)　279-283　2004年6月

ISSN：0966-0844
The detection of bovine lactoferrin binding protein on Trypanosoma brucei 査読有り

T Tanaka, Y Abe, N Inoue, WS Kim, H Kumura, H Nagasawa, Igarashi, I, K Shimazaki

JOURNAL OF VETERINARY MEDICAL SCIENCE　66　(6)　619-625　2004年6月

DOI： 10.1292/jvms.66.619 　

ISSN：0916-7250
Transferrin family proteins bind to Bifidobacterium spp.

WS Kim, T Tanaka, K Shimazaki

MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL　59　(9-10)　491-494　2004年

ISSN：0026-3788
Detection of binding-protein in Bifidobacterium bifidum Bb-11 by various kinds of lactoferrin

WS Kim, T Tanaka, K Shimazaki

MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL　59　(7-8)　375-377　2004年

ISSN：0026-3788
Comparison of Reactivity of Polyclonal and Monoclonal Antibodies against Bovine Lactoferrin and its Fragments (ラクトフェリン研究--基礎から応用への掛け橋)

Tun Khin Mg, 田仲哲也, 玖村朗人

ミルクサイエンス　53　(4)　239-241　2004年
出版者・発行元：日本酪農科学会
ISSN：1343-0289
Partial characterization of dextran-degrading enzyme obtained from blue cheese

Y. Wang, A. Suzuki, T. Tanaka, H. Kumura, K. Shimazaki

Journal of Dairy Science　87　(6)　1627-1633　2004年
出版者・発行元： American Dairy Science Association
DOI： 10.3168/jds.S0022-0302(04)73317-2 　

ISSN：0022-0302
Screening of dairy yeast strains for probiotic applications

H. Kumura, Y. Tanoue, M. Tsukahara, T. Tanaka, K. Shimazaki

Journal of Dairy Science　87　(12)　4050-4056　2004年
出版者・発行元： American Dairy Science Association
DOI： 10.3168/jds.S0022-0302(04)73546-8 　

ISSN：0022-0302
The detection of bovine lactoferrin binding protein on Toxoplasma gondii 査読有り

T Tanaka, Y Abe, WS Kim, Xuan, X, H Nagasawa, Igarashi, I, H Kumura, K Shimazaki

JOURNAL OF VETERINARY MEDICAL SCIENCE　65　(12)　1377-1380　2003年12月

DOI： 10.1292/jvms.65.1377 　

ISSN：0916-7250
Antiviral activity of lactoferrin against canine herpesvirus. 国際誌査読有り

Tetsuya Tanaka, Syogo Nakatani, Xuenan Xuan, Haruto Kumura, Ikuo Igarashi, Kei ichi Shimazaki

Antiviral research　60　(3)　193-9　2003年11月

DOI： 10.1016/S0166-3542(03)00157-8 　

ISSN：0166-3542
Expression and characterization of bovine lactoperoxidase by recombinant baculovirus 査読有り

T Tanaka, S Sato, H Kumura, K Shimazaki

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　67　(10)　2254-2261　2003年10月

DOI： 10.1271/bbb.67.2254 　

ISSN：0916-8451

eISSN：1347-6947
Expression of bovine lactoferrin and lactoferrin N-lobe by recombinant baculovirus and its antimicrobial activity against Prototheca zopfii 査読有り

T Tanaka, Nakamura, I, NY Lee, H Kumura, K Shimazaki

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE　81　(5)　349-354　2003年10月

DOI： 10.1139/O03-062 　

ISSN：0829-8211
Preliminary study and characterization of dextran degradation by the water-soluble fraction extracted from blue cheese 査読有り

K Shimazaki, S Maki, Y Wang, M Sato, T Tanaka, H Kumura

MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL　58　(7-8)　379-382　2003年

ISSN：0026-3788
Casein digestion by Debaryomyces hansenii isolated from cheese 査読有り

H Kumura, K Takagaki, T Sone, M Tsukahara, T Tanaka, K Shimazaki

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　66　(6)　1370-1373　2002年6月

DOI： 10.1271/bbb.66.1370 　

ISSN：0916-8451

eISSN：1347-6947
Fine structures of epitopic sites in human and bovine lactoferrin recognized by anti-bovine lactoferrin C-lobe monoclonal antibody

MS Nam, M Kamio, KI Shimazaki, S Harakawa, T Tanaka, Y Omata, A Saito, H Kumura, Igarashi, I, N Suzuki

FOOD AND AGRICULTURAL IMMUNOLOGY　14　(2)　139-146　2002年6月

DOI： 10.1080/09540100220145160 　

ISSN：0954-0105
Lactoferrin-binding proteins in Bifidobacterium bifidum 査読有り

WS Kim, T Tanaka, H Kumura, K Shimazaki

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE　80　(1)　91-94　2002年2月

ISSN：0829-8211

eISSN：1208-6002
Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1 査読有り

XN Xuan, Igarashi, I, T Tanaka, S Fukumoto, H Nagasawa, K Fujisaki, T Mikami

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY　8　(3)　645-646　2001年5月

DOI： 10.1128/CDLI.8.3.645-646.2001 　

ISSN：1071-412X
Expression of Babesia equi merozoite antigen 1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay

XA Xuan, A Larsen, H Ikadai, T Tanaka, Igarashi, I, H Nagasawa, K Fujisaki, Y Toyoda, N Suzuki, T Mikami

JOURNAL OF CLINICAL MICROBIOLOGY　39　(2)　705-709　2001年2月

DOI： 10.1128/JCM.39.2.705-709.2001 　

ISSN：0095-1137
The role of CD4(+) or CD8(+) T cells in the protective immune response of BALB/c mice to Neospora caninum infection

T Tanaka, T Hamada, N Inoue, H Nagasawa, K Fujisaki, N Suzuki, T Mikami

VETERINARY PARASITOLOGY　90　(3)　183-191　2000年6月

DOI： 10.1016/S0304-4017(00)00238-7 　

ISSN：0304-4017
Growth-inhibitory effects of interferon-γ on Neospora caninum in murine macrophages by a nitric oxide mechanism

Tetsuya Tanaka, Hideyuki Nagasawa, Kozo Fujisaki, Naoyoshi Suzuki, Takeshi Mikami

Parasitology Research　86　(9)　768-771　2000年
出版者・発行元：
DOI： 10.1007/s004360000242 　

ISSN：0932-0113
Detection of Antibodies to Babesia equi by the Latex Agglutination Test with Recombinant Merozoite Antigen-2

T. Tanaka, X. Xuan, I. Igarashi, H. Nagasawa, K. Fujisaki, N. Suzuki, T. Mikami

Journal of Protozoology Research　9　152-156　1999年12月1日

ISSN：0917-4427
Expression of Recombinant Toxoplasma gondii P24 査読有り

Tetsuya Tanaka, Xuenan Xuan, Mihoko Kato, Hideyuki Nagasawa, Kozo Fujisaki, Takeshi Mikami, Naoyoshi Suzuki

Journal of Veterinary Medical Science　61　(11)　1235-1239　1999年11月

ISSN：1782-155X
Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA 査読有り

Tetsuya Tanaka, Xuenan Xuan, Hiromi Ikadai, Ikuo Igarashi, Hideyuki Nagasawa, Kozo Fujisaki, Takeshi Mikami, Naoyoshi Suzuki

International Journal for Parasitology　29　(11)　1803-1808　1999年11月

DOI： 10.1016/S0020-7519(99)00146-0 　

ISSN：0020-7519
Expression of recombinant Toxoplasma gondii P24

T Tanaka, Xuan, X, M Kato, H Nagasawa, K Fujisaki, T Mikami, N Suzuki

JOURNAL OF VETERINARY MEDICAL SCIENCE　61　(11)　1235-1239　1999年11月

ISSN：0916-7250

eISSN：1347-7439
Growth Inhibitory Effect of Bovine Lactoferrin to Toxoplasma gondii Tachyzoites in Murine Macrophages: Tyrosine Phosphorylation in Murine Macrophages Induced by Bovine Lactoferrin

Tetsuya Tanaka, Yoshitaka Omata, Tsuya Isamida, Atsushi Saito, Keiichi Shimazaki, Koji Yamauchi, Naoyoshi Suzuki

Journal of Veterinary Medical Science　60　(3)　369-371　1998年
出版者・発行元： Maruzen Co. Ltd.
DOI： 10.1292/jvms.60.369 　

ISSN：0916-7250
Protective Effect of Lactoferricin against Toxoplasma gondii Infection in Mice

Tsuya Isamida, Tetsuya Tanaka, Yoshitaka Omata, Koji Yamauchi, Kei-Ichi Shimazaki, Atsushi Saito

Journal of Veterinary Medical Science　60　(2)　241-244　1998年
出版者・発行元： Maruzen Co. Ltd.
DOI： 10.1292/jvms.60.241 　

ISSN：0916-7250
Structural and immunochemical studies on bovine lactoferrin fragments

K Shimazaki, M Kamio, MS Nam, S Harakawa, T Tanaka, Y Omata, A Saito, H Kumura, K Mikawa, Igarashi, I, N Suzuki

ADVANCES IN LACTOFERRIN RESEARCH　443　41-48　1998年

ISSN：0065-2598
Growth inhibitory effect of bovine lactoferrin on Toxoplasma gondii tachyzoites in murine macrophages: Role of radical oxygen and inorganic nitrogen oxide in Toxoplasma growth-inhibitory activity

T Tanaka, Y Omata, M Narisawa, A Saito, K Shimazaki, Igarashi, I, H Hirumi, N Suzuki

VETERINARY PARASITOLOGY　68　(1-2)　27-33　1997年1月

DOI： 10.1016/S0304-4017(96)01069-2 　

ISSN：0304-4017
抗ラクトフェリシンモノクローナル抗体の作成とエピトープの推定査読有り

島崎敬一, Nam Myoung Soo, 原川信二, 田仲哲也, 小俣吉孝, 齋藤篤志, 玖村朗人, 三河勝彦, 五十嵐郁夫, 鈴木直義

The journal of veterinary medical science　58　(12)　1227-1229　1996年12月

ISSN：0916-7250
ウシラクトフェリンのToxoplasma gondii増殖抑制効果査読有り

田仲哲也, 小俣吉孝, 齋藤篤志

The Journal of Veterinary Medical Science　58　(1)　61-65　1996年1月25日
出版者・発行元： (公社)日本獣医学会
ISSN：0916-7250

詳細を見る詳細を閉じる

Toxoplasma gondii虫体の細胞内増殖に対し, ウシラクトフェリン(LF)の及ぼす影響について検討すべく, マウス腹腔内マクロファージ及びマウス胎仔細胞を用い, LF添加培養時における虫体の細胞内増殖について経時的観察を行った. 虫体の細胞内侵入能力には, LFの添加は影響を示さなかった. 一方, 虫体の細胞内増殖に対してはLF添加により阻害効果が観察された. アポ型LF添加並びにホロ型LF添加においても同様の阻害効果が観察された事から, LFによる阻害効果には鉄分子は関与しない事が示唆された. また, LF前培養マクロファージでは, 増殖阻害を示さなかったのに対し, 同処理胎仔細胞では虫体の増殖阻害を示した事から, 両者におけるLFの効果は異なる機序が推察された.
Toxoplasma gondii: Parasiticidal effects of bovine lactoferricin against parasites 査読有り

Tetsuya Tanaka, Yoshitaka Omata, Atushi Saito, Keiichi Shimazaki, Koji Yamauchi, Mitsunori Takase, Kouzou Kawase, Ikuo Igarashi, Naoyoshi Suzuki

Experimental Parasitology　81　(4)　614-617　1995年

DOI： 10.1006/expr.1995.1157 　

ISSN：0014-4894
SEPARATION OF TRYPTIC FRAGMENTS OF BOVINE LACTOFERRIN AND THEIR ABILITY TO BIND TO TRYPANOSOMA-CRUZI 査読有り

K SHIMAZAKI, T TANAKA, IGARASHI, I, A SAITO, H KANBARA, N SUZUKI

INDIGENOUS ANTIMICROBIAL AGENTS OF MILK - RECENT DEVELOPMENTS　122-130　1994年
Separation and Characterization of the C-Terminal Half Molecule of Bovine Lactoferrin 査読有り

Kei-Ichi Shimazaki, Tetsuya Tanaka, Hiroshi Kon, Kazunary Oota, Ayano Kawaguchi, Yoshiyuki Maki, Tetsuya Sato, Yukari Ueda, Toshio Tomimura, Seiichi Shimamura

Journal of Dairy Science　76　(4)　946-955　1993年

DOI： 10.3168/jds.S0022-0302(93)77421-4 　

ISSN：0022-0302

︎全件表示 ︎最初の5件までを表示

MISC 123

マダニから分離した糸状菌の二次代謝ポテンシャルの開拓に関する研究

土岐俊輔, 森下陽平, 本間悠人, 古村翔, 二神泰基, 田仲哲也, 尾崎太郎, 浅井禎吾

日本薬学会年会要旨集(Web)　146th　2026年

ISSN： 0918-9823
ウシ乳房炎由来の黄色ブドウ球菌が特異的に分泌する凝乳タンパク質の解析

間嶋朱里, 金森萌, 下田蒼, 松田敬一, 金子淳, 米山裕, 田仲哲也, 戸部隆太

東北畜産学会報　75　(2)　31-31　2025年8月
出版者・発行元：東北畜産学会
ISSN： 1341-626X
抗菌ペプチドPersulcatusinおよびMyticofensin A2の微生物発現系構築と機能特性の解析

渡辺朋弥, 金井大地, 下田蒼, 米山裕, 田仲哲也, 戸部隆太

日本農芸化学会東北支部大会プログラム・講演要旨集　160th　2025年
ウシ乳房炎由来の黄色ブドウ球菌が特異的に分泌する凝乳因子の誘導機構

金森萌, 間嶋朱里, 下田蒼, 松田敬一, 栗原達夫, 金子淳, 米山裕, 田仲哲也, 戸部隆太

日本農芸化学会大会講演要旨集(Web)　2025　2025年

ISSN： 2186-7976
ウシ乳房炎由来の黄色ブドウ球菌が示す「凝乳」による薬剤回避機能

間嶋朱里, 下田蒼, 松田敬一, 栗原達夫, 金子淳, 米山裕, 田仲哲也, 金森萌, 戸部隆太

日本農芸化学会大会講演要旨集(Web)　2025　2025年

ISSN： 2186-7976
フタトゲチマダニにおけるアクアポリンの機能解析

佐藤萌子, 佐藤成子, 水野寛太, 田仲哲也, XUAN X., 鈴木宏志, 白藤(梅宮)梨可

日本ダニ学会誌　33　(1)　2024年

ISSN： 0918-1067
鹿児島県大隅地域におけるRickettsia spp.の疫学調査ならびにgltA遺伝子を標的とした分子系統解析

鈴一之将, 横山雅大, 芦原誠一, 榮村奈緒子, 田仲哲也

衛生動物　74　(2)　85-85　2023年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
鹿児島県大隅地域の異なる環境におけるマダニ類の出現状況とリケッチア保有率

栄村奈緒子, 横山雅大, 畑邦彦, 芦原誠一, 鈴一之将, 田仲哲也

日本生態学会大会講演要旨(Web)　70th　2023年
マダニ唾液由来HISG-g22様タンパク質の計算化学的解析

川田逸人, 川田逸人, 小池優貴, 佐々木紗己, 中島政斗, 佐々木萌可, 上松史尚, 三角修斗, KWOFIE Kofi Dadzie, LADZEKPO Danielle, LADZEKPO Danielle, BEHRI Meryem, 井上貴裕, 神保恵, 三上房子, 田仲哲也, 辻尚利, 辻尚利, 八田岳士, 八田岳士

日本寄生虫学会大会プログラム・抄録集　92nd　2023年

ISSN： 1348-4613
リコンビナーゼポリメラーゼ増幅を用いた牛の小型ピロプラズマ病の迅速的な遺伝子検出法の開発

樋ノ口峰大, 叶有斗, 志賀慧, 堀江真行, 一二三達郎, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　92nd　2023年

ISSN： 1348-4613
マダニ胚発生におけるフェリチンの発現動態

島崎慧, HERNANDEZ Emmanuel, HERNANDEZ Emmanuel, 新原博子, 白藤(梅宮)梨可, 藤崎幸蔵, 田仲哲也

日本分子生物学会年会プログラム・要旨集(Web)　46th　2023年
鹿児島県大隅地域の異なる環境におけるマダニ類の季節消長とリケッチア保有状況

榮村奈緒子, 鈴一之将, 横山雅大, 木村仁美, 芦原誠一, 畑邦彦, 一二三達郎, 田仲哲也

九州森林学会大会(Web)　79th　2023年
日本の食用馬の多包虫症における多包条虫のミトコンドリア・シトクロームb(cob)遺伝子を標的とした分子系統解析

佐藤美穂, 一二三達郎, 田仲哲也, 鈴一之将, 秋岡幸兵, 藤又千晶, 新飼竜平, 前田勉, 草木迫浩大, 筏井宏実, 三好宣彰

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2023　2023年
鹿児島県大隅地域から検出されたRickettsia spp.におけるgltA,ompB,htrA遺伝子の分子疫学的特徴

鈴一之将, 木村仁美, 芦原誠一, 榮村奈緒子, 一二三達郎, 田仲哲也

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2023　2023年
マダニにおけるReMOT Controlの試み(Attempt of ReMOT Control for tick, Haemaphysalis longicornis)

Hernandez Emmanuel Pacia, 川田逸人, 辻尚利, 田仲哲也, 八田岳士

衛生動物　73　(Suppl.)　56-56　2022年3月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ胚発生におけるフェリチンの発現動態

島崎慧, ヘルナンデズエマヌエル, ヘルナンデズエマヌエル, 新原博子, 白藤(梅宮)梨可, 藤崎幸蔵, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　91st　2022年

ISSN： 1348-4613
ダニ媒介性ウイルスのマダニ感染モデルの構築および感染・増殖機構の解析

田仲哲也, 好井健太朗

長崎大学熱帯医学研究拠点共同研究報告集　2021　2022年
リコンビナーゼポリメラーゼ増幅法を用いた牛のTheileria orientalis遺伝子型の同定

樋ノ口峰大, 叶有斗, 志賀慧, 堀江真行, 一二三達郎, 田仲哲也

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2022 (CD-ROM)　2022年
馬の多包虫症におけるリコンビナーゼポリメラーゼ増幅法を用いた迅速な診断法の開発

佐藤美穂, 佐藤美穂, 一二三達郎, 一二三達郎, 田仲哲也, 秋岡幸兵, 藤又千晶, 三好宣彰

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2022 (CD-ROM)　2022年
Haemaphysalis longicornisに由来するelongation factor I-α(EFIα)のプロモータ分析(Promoter analysis of elongation factor I-alpha(EFI α) derived from Haemaphysalis longicornis)

Hernandez Emmanuel Pacia, 川田逸人, 山本大介, 加藤大智, 松林誠, 田仲哲也, 辻尚利, 八田岳士

日本寄生虫学会・日本臨床寄生虫学会合同大会プログラム・抄録集　90回・32回　58-58　2021年4月
出版者・発行元：日本寄生虫学会・日本臨床寄生虫学会
馬の多包虫症におけるLAMP法を用いた迅速的な診断方法の開発

一二三達郎, 一二三達郎, 秋岡幸兵, 田仲哲也, 三好宣彰

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2021　2021年
マダニフェリチンの機能解明

田仲哲也

衛生動物　72　(Supplement)　2021年

ISSN： 0424-7086
ダニ研究の最前線とダニ媒介性感染症制御の可能性を探るマダニの吸血生理と抗マダニワクチン

田仲哲也

衛生動物　71　(2)　57-64　2020年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ由来カタラーゼ遺伝子の同定ならびに組換え体を用いた抗酸化活性の検証

草木迫浩大, 中尾亮, 田仲哲也, 野中成晃

衛生動物　71　(2)　113-113　2020年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
ランガットウイルスに感染したフタトゲチマダニにおける抗酸化分子の発現動態

山田智大, Pacia Hernandez Emmanuel, 新原博子, 藤崎幸蔵, 好井健太郎, 田仲哲也

衛生動物　71　(2)　132-132　2020年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ由来カタラーゼ遺伝子の同定ならびに組換え体を用いた抗酸化活性の検証

草木迫浩大, 中尾亮, 田仲哲也, 野中成晃

衛生動物　71　(2)　113-113　2020年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
単為生殖系フタトゲチマダニ胚発生における鉄イオンの動態と抗酸化分子の局在

島崎慧, Pacia Hernandez Emmanuel, 新原博子, 藤崎幸蔵, 田仲哲也

衛生動物　71　(2)　132-132　2020年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
ランガットウイルスに感染したフタトゲチマダニにおける抗酸化分子の発現動態

山田智大, Pacia Hernandez Emmanuel, 新原博子, 藤崎幸蔵, 好井健太郎, 田仲哲也

衛生動物　71　(2)　132-132　2020年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ伸長因子1α(EF1α)遺伝子の上流領域解析(Promoter analysis of elongation factor 1-alpha(EF1α) derived from Haemaphysalis longicornis tick)

エマニュエル・ヘルナンデス, コフィ・コフィ, 坪川大悟, 三上房子, 松林誠, 辻尚利, 田仲哲也, 八田岳士

衛生動物　71　(Suppl.)　52-52　2020年3月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ胚発生における鉄イオンの動態と抗酸化分子の局在

島崎慧, HERNANDEZ Emmanuel, 新原博子, 白藤(梅宮)梨可, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　163rd　2020年

ISSN： 1347-8621
マダニ胚由来細胞におけるフタトゲチマダニプロモータ活性の評価

HERNANDEZ Emmanuel Pacia, HERNANDEZ Emmanuel Pacia, 草木迫浩大, 八田岳士, 田仲哲也

日本分子生物学会年会プログラム・要旨集(Web)　43rd　2020年
グルタチオンSトランスフェラーゼはフタトゲチマダニの酸化ストレス管理とダニ駆除剤代謝に重要である(Glutathione S-transferases are important in oxidative stress management and acaricide metabolism in the hard ticks Haemaphysalis longicornis)

Hernandez Emmanuel, 草木迫浩大, Talactac Melbourne, Galay Remil, 八田岳士, 藤崎幸蔵, 辻尚利, 田仲哲也

日本獣医学会学術集会講演要旨集　162回　218-218　2019年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
単為生殖系フタトゲチマダニの胚発生における抗酸化分子の発現プロファイルの作成

島崎慧, Hernandez Emmanuel Pacia, 新原博子, 藤崎幸蔵, 田仲哲也

衛生動物　70　(2)　107-107　2019年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ由来抗酸化蛋白質を発現する哺乳類細胞の発現安定株の作製

諸熊遥樹, 草木迫浩大, Talactac Melbourne R., Hernandez Emmanuel P., 田仲哲也

衛生動物　70　(2)　107-107　2019年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
カナダから輸入された馬にみられた肝臓灰白色硬結節と多包虫感染との関連性

一二三達郎, 一二三達郎, 田仲哲也, HERNANDEZ Emmanuel Pacia, 秋岡幸兵, 山田薫理, 今村安孝, 畑井仁, 三好宣彰

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2019　2019年
哺乳類細胞におけるマダニアクチン遺伝子プロモーターを用いた外来遺伝子の発現

諸熊遥樹, 草木迫浩大, Talactac Melbourne R., Hernandez Emmanuel P., 田仲哲也

衛生動物　69　(2)　123-123　2018年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
マダニ胚由来細胞における遺伝子発現ベクターの探索並びに構築

田仲哲也, 井戸明子, 草木迫浩大, 諸熊遥樹, 正谷達謄, Hernandez Emmanuel, Talactac Melbourne, 好井健太朗

生命科学系学会合同年次大会　2017年度　[1P-1317]　2017年12月
出版者・発行元：生命科学系学会合同年次大会運営事務局
マダニ‐バベシア実験感染モデルの確立

前田大輝, 前田大輝, 前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林誠, 三好猛晴, 白藤(梅宮, 梨可, 河津信一郎, 五十嵐郁男, 望月雅美, 辻尚利, 田仲哲也

日本獣医学会学術集会講演要旨集　160th　203-203　2017年8月30日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
シカダニIxodes scapularis胚由来細胞株(ISE6)におけるFerritin 1発現誘導(Induction of Ferritin1 expression in Ixodes scapularis embryo derived cell line(ISE6))

Hernandez Emmanuel, 草木迫浩大, Galay Remil, Talactac Melbourne, 好井健太朗, 田仲哲也

日本獣医学会学術集会講演要旨集　160回　346-346　2017年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニHaemaphysalis longicornisにおいて新規同定されたディフェンシン様ペプチドHEdefensinの性状解析と抗ウイルス活性(Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis)

Talactac Melbourne, 屋田友里花, 好井健太朗, Hernandez Emmanuel, 草木迫浩大, 前田大輝, 藤崎幸蔵, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　160回　390-390　2017年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
マダニペルオキシレドキシンの特性解明(ペルオキシレドキシンの性状解析並びに吸血・産卵に果たす役割について)

草木迫浩大, 正谷達謄, 宮田健, Galay Remil, 白藤梨可, 宮, 前田大輝, Talactac Melbourne, Hernandez Emmanuel, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　160回　202-202　2017年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
マダニ胚由来細胞における遺伝子発現ベクターの検証

井戸明子, 正谷達謄, 好井健太朗, Galay Remil L., 草木迫浩大, Talactac Melbourne R., Hernandez Emmanuel P., 望月雅美, 田仲哲也

衛生動物　68　(2)　75-75　2017年7月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
ペルオキシレドキシンは連携して細胞内過酸化水素濃度を制御する

草木迫浩大, HERNANDEZ Emmanuel P., 草木迫浩大, HERNANDEZ Emmanuel P., TALACTAC Melbourne R., TALACTAC Melbourne R., TALACTAC Melbourne R., 好井健太朗, 田仲哲也, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　86th　2017年

ISSN： 1348-4613
マダニの酸化ストレス制御分子を標的とした抗マダニワクチンの開発

田仲哲也, 草木迫浩大, 宮田健

食肉に関する助成研究調査成果報告書　34　253‐257　2016年11月
Babesia ovata原虫におけるマダニ中腸形態へのステージシフト誘導実験

前田大輝, 前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林誠, 白藤(梅宮)梨可, 辻尚利, 田仲哲也, 田仲哲也

日本獣医学会学術集会講演要旨集　159th　334-334　2016年8月30日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
鹿児島県各地で採取されたマダニにおけるマダニ媒介性原虫の分子疫学的解析

正谷達謄, 林慶, 立野守洋, 遠藤泰之, 松尾智英, 田仲哲也, 御供田睦代, 安藤匡子

日本獣医学会学術集会講演要旨集　159th　338-338　2016年8月30日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
マダニISE6細胞を用いた過酸化水素発生の可視化についての検討

草木迫浩大, Talactac Melbourne, Hernandez Emmanuel, 前田大輝, 好井健太朗, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　159回　339-339　2016年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
Haemaphysalis longicornisにおけるThogotoウイルス(HI-Kamigamo-25)日本単離株の生存動態(Survival dynamics of Japan isolate Thogoto virus(HI-Kamigamo-25) in Haemaphysalis longicornis)

Talactac Melbourne, 好井健太朗, Hernandez Emmanuel, 草木迫浩大, 前田大輝, 藤崎幸蔵, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　159回　383-383　2016年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
鹿児島県各地で採取されたマダニにおけるTheileria,Babesia及びHepatozoonの分子疫学

正谷達謄, 林慶, 立野守洋, 遠藤泰之, 松尾智英, 田仲哲也, 御供田睦代, 関(市川)まどか, 安藤匡子

日本寄生虫学会大会プログラム・抄録集　85th　83　2016年2月

ISSN： 1348-4613
汎用的な細胞凍結保存液を用いたBabesia gibsoni原虫凍結保存法の検討およびその改良

草木迫浩大, 草木迫浩大, 正谷達謄, 屋田友里花, TALACTAC Melbourne Rio, TALACTAC Melbourne Rio, HERNANDEZ Emmanuel Pacia, 前田大輝, 前田大輝, 望月雅美, 望月雅美, 田仲哲也, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　85th　2016年

ISSN： 1348-4613
フタトゲチマダニ体内におけるBabesia ovata移行動態について

前田大輝, 前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林誠, 三好猛晴, 白藤(梅宮)梨可, 大久保和洋, 五十嵐郁男, 望月雅美, 望月雅美, 藤崎幸蔵, 辻尚利, 田仲哲也, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　85th　2016年

ISSN： 1348-4613
過酸化水素消去酵素2-Cysペルオキシレドキシンはマダニの吸血および産卵時の抗酸化機構に関与する

田仲哲也, 草木迫浩大, 正谷達謄, 宮田健, Galay Remil Linggatong, 白藤梨可, 宮, 前田大輝, Talactac Melbourne Rio, 辻尚利, 望月雅美, 藤崎幸蔵

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　88回・38回　[4T10L-07(3P0466)]　2015年12月
出版者・発行元： (公社)日本生化学会
ベクターマダニ体内におけるBabesia ovata増殖動態についての一考察

前田大輝, 前田大輝, 八田岳士, 松林誠, 三好猛晴, 白藤(梅宮, 梨可, 大久保和洋, 五十嵐郁男, 辻尚利, 望月雅美, 望月雅美, 藤崎幸蔵, 田仲哲也, 田仲哲也

日本獣医学会学術集会講演要旨集　158th　302-302　2015年8月30日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニにおけるペルオキシレドキシンの役割について

草木迫浩大, GALAY Remil Linggatong, 白藤(梅宮)梨可, 前田大輝, TALACTAC Melbourne Rio, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　158th　301-301　2015年8月30日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
ダニ媒介性脳炎ウイルスの代替としてのLangatウイルスに対するHaemaphysalis longicornisロンギシンP4ペプチドの殺ウイルス活性(Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus)

Talactac Melbourne Rio, 好井健太朗, 辻尚利, 藤崎幸蔵, 田仲哲也, 望月雅美

日本獣医学会学術集会講演要旨集　158回　345-345　2015年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニ由来2-Cys型ペルオキシレドキシン(HIPrx2)の抗酸化部位の検索

草木迫浩大, 正谷達謄, 宮田健, GALAY Remil Linggatong, 前田大輝, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　84th　2015年

ISSN： 1348-4613
フタトゲチマダニ(Haemaphysalis longicornis)におけるフェリチン類の重要な機能とダニ駆除の標的分子としての可能性(The critical functions of ferritins in Haemaphysalis longicornis and their potential as target molecules for tick control)

Galay Remil Linggatong, 白藤梨可, 宮, 宮田健, 草木迫浩大, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　157回　238-238　2014年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
マウス寄生性コクシジウムEimeria krijgsmanniの生活環に関する形態学的観察

小野結菜, 松林誠, 川口博明, 辻尾祐志, 田仲哲也, 三好宣彰, 松井利博, 松尾智英

日本獣医学会学術集会講演要旨集　157回　355-355　2014年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニ脂肪体の卵黄タンパク質前駆体合成におけるtarget of rapamycin(TOR)の役割

白藤(梅宮)梨可, DAMDMSUREN Boldbaatar, MIN Liao, BANZRAGCH Battur, MORSHEDUR Rahman Md, THASANEEYA Kuboki, LINGGATONG, Galay Remil, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　83rd　79　2014年2月

ISSN： 1348-4613
フタトゲチマダニ由来2-Cys型ペルオキシレドキシンの機能解析

草木迫浩大, 草木迫浩大, 正谷達謄, GALAY Remil Linggatong, GALAY Remil Linggatong, 前田大輝, 前田大輝, 望月雅美, 望月雅美, 藤崎幸蔵, 田仲哲也, 田仲哲也

日本獣医学会学術集会講演要旨集　157th　2014年

ISSN： 1347-8621
フタトゲチマダニ由来ロイシンリッチリピートドメイン保有蛋白質の動態と機能解析

栗巣孔士, 前田大輝, REMIL Linggatong Galay, 武智理恵, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　65　(2)　2014年

ISSN： 0424-7086
フタトゲチマダニ由来TNF Receptor Associated Factor(TRAF)の同定とその役割について

武智理恵, GALAY Remil Linggatong, 前田大輝, 草木迫浩大, 松尾智英, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　157th　2014年

ISSN： 1347-8621
フタトゲチマダニ由来TNF Receptor Associated Factorの同定とその役割について

武智理恵, REMIL Linggatong Galay, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　65　(2)　2014年

ISSN： 0424-7086
ラクトフェリンによる抗マラリア原虫機能の解析

小森園亮, 柿本麻美子, 田仲哲也, LI Changchun, 渡部久実, HISHAM Ibrahim, 若林裕之, 山内恒治, 宮田健

日本フードファクター学会学術集会講演要旨集　19th　112　2014年
フタトゲチマダニ体内におけるB.microtiの検出

草木迫浩大, 前田大輝, GALAY Remil Linggatong, 松尾智英, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　156th　236-236　2013年8月30日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニ由来C型レクチン様蛋白質の同定とその特性解明

前田大輝, 草木迫浩大, GALAY Remil Linggatong, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　156th　235-235　2013年8月30日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニHaemaphysalis longicornisにおいてフェリチンは鉄介在性酸化ストレスを防御する(Ferritins protect the hard tick Haemaphysalis longicornis from iron-mediated oxidative stress)

Galay Remil Linggatong, 草木迫浩大, 前田大輝, 白藤梨可, 梅宮, Tan Bacolod Eugene, 小山次郎, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　156回　235-235　2013年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニロンギシンにおける抗バベシア活性を持つペプチド部位の検索

田仲哲也, GALAY Remil Linggatong, 前田大輝, MIN Aung Kyaw, 白藤(梅宮)梨可, 玄学南, 五十嵐郁男, 望月雅美, 辻尚利, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　155th　217-217　2013年3月4日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
マダニの卵黄タンパク質前駆体合成を制御する分子機構の解明

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, LIAO Min, BATTUR Banzragch, RAHMAN Morshedur Md, KUBOKI Thasaneeya, GALAY Remil Linggatong, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　155th　82-82　2013年3月4日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニにおけるウサギ血液由来トランスフェリンの動態.

森寛行, GALAY R. L., 前田大輝, 松尾智英, 白藤(梅宮)梨可, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　64　(2)　2013年

ISSN： 0424-7086
フタトゲチマダニの鉄依存性酸化ストレスから保護するフェリチンの重要性

田仲哲也, GALAY Remil L, 白藤(梅宮)梨可, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵

日本分子生物学会年会プログラム・要旨集(Web)　36th　2P-0295 (WEB ONLY)　2013年
マダニのオートファジー関連遺伝子とその役割

白藤梨可, 梅宮, 川野優, D, ボルドバータル, 松尾智英, 田仲哲也, 藤崎幸蔵

獣医寄生虫学会誌　11　(1)　25-25　2012年10月
出版者・発行元：日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニにおける宿主血液由来トランスフェリンの動態について

田仲哲也, 森寛行, R.L.・ガレイ, 前田大輝, 白藤梨可, 宮, 藤崎幸蔵

獣医寄生虫学会誌　11　(1)　52-52　2012年10月
出版者・発行元：日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニ由来サイクロフィリンAとバベシア原虫の関連性

前田大輝, DAMDINSUREN Boldbaatar, 草木迫浩大, KYAW MIN Aung, GALAY Remil, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　154th　216-216　2012年8月31日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニ卵黄タンパク質前駆体合成におけるTOR阻害剤ラパマイシンの影響

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　154th　204-204　2012年8月31日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
カタダニHaemaphysalis longicornisの吸血と生殖の遂行における鉄蓄積タンパク質であるフェリチンの重要性(The importance of iron storage protein ferritin on successfu blood feeding and reproduction of the hard tick Haemaphysalis longicornis)

Galay Remil, Aung Kyaw Min, 白藤梨可, 宮, 前田大輝, 松尾智英, 川口博明, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　154回　203-203　2012年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニ由来サイクロフィリンAの抗バベシア原虫作用

前田大輝, 田仲哲也, BOLDBAATAR D, 草木迫浩大, AUNG KM, GALAY RL, 白藤(梅宮)梨可, 藤崎幸蔵

衛生動物　63　(2)　166-166　2012年6月30日
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086
微生物貪食におけるフタトゲチマダニクラスBスカベンジャー受容体の重要性

キャウ・ミン・アウン, ダミディスレン・ボルドバータル, 白藤梨可, 宮, ガライ・レミル・リンガートン, 久米愛子, 田仲哲也, 藤崎幸蔵

衛生動物　63　(2)　166-166　2012年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086
フタトゲチマダニにおける宿主血液由来トランスフェリンの動態について

田仲哲也, 森寛行, GALAY Remil Linggatong, 前田大輝, 白藤(梅宮)梨可, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　153rd　225-225　2012年3月1日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
マダニのオートファジー関連遺伝子とその役割

白藤(梅宮)梨可, 川野優, BOLDBAATAR Damdinsuren, 松尾智英, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　153rd　111-111　2012年3月1日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニの吸血および産卵におけるフェリチンの重要性

GALAY Remil L, KYAW MIN Aung, 白藤(梅宮)梨可, 前田大輝, 松尾智英, 川口博明, 三好宣彰, 鈴木宏志, XUAN Xuenan, 望月雅美, 藤崎幸蔵, 田仲哲也

日本分子生物学会年会プログラム・要旨集(Web)　35th　2P-0080 (WEB ONLY)　2012年
フタトゲチマダニにおけるオートファジー関連遺伝子HlATG6発現抑制の産卵への影響

川野優, 白藤(梅宮)梨可, BOLDOBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　62　(2)　158-158　2011年6月15日
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086
フタトゲチマダニにおけるセリン/スレオニンキナーゼAktの発現及び機能解析

田中徹, 白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　62　(2)　158-158　2011年6月15日
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086
TOR経路はフタトゲチマダニのビテロジェニン合成に必須である.

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　62　(2)　157-157　2011年6月15日
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086
フタトゲチマダニにおけるクラスBスカベンジャー受容体CD36の機能解析

Aung Kyaw Min, Boldobaatar Damdinsuren, 白藤梨可, 宮, 田仲哲也, 藤崎幸蔵

衛生動物　62　(2)　158-158　2011年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086
フタトゲチマダニロンギシンのトキソプラズマ原虫に及ぼす影響について

田仲哲也, 前田大輝, 松尾智英, DAMDINSUREN Boldbaatar, 白藤(梅宮)梨可, 玄学南, 辻尚利, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　151st　214-214　2011年3月1日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
マダニにおけるオートファジーの役割

白藤(梅宮)梨可, 松尾智英, 川野優, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　151st　107-107　2011年3月1日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニのビテロジェニン合成におけるTOR経路の役割

白藤梨可, 梅宮, ボルドバータル D, 田仲哲也, 藤崎幸蔵

獣医寄生虫学会誌　9　(2)　169-169　2011年3月
出版者・発行元：日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニ由来クラスBスカベンジャー受容体CD36の同定とその特性(Identification and characterization of class B scavenger receptor CD 36 from the hard tick, Haemaphysalis longicornis)

アウン K.M, ボルドバータル D, 寥敏, 白藤梨可, 宮, 中尾純大, 松岡輝重, 田仲哲也, 藤崎幸蔵

獣医寄生虫学会誌　9　(2)　170-170　2011年3月
出版者・発行元：日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニにおけるオートファジー関連遺伝子H1ATG6の機能解析

川野優, 白藤梨可, 宮, ボルドバータル D, 田仲哲也, 藤崎幸蔵

獣医寄生虫学会誌　9　(2)　171-171　2011年3月
出版者・発行元：日本獣医寄生虫学会
ISSN： 1347-961X
elF4E遺伝子の機能解明;フタトゲチマダニの生殖系細胞の発育に必須であると思われる

高澤木綿子, BOLDBAATAR Damdinsuren, 久米愛子, 白藤(梅宮)梨可, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　80th　84　2011年2月

ISSN： 1348-4613
フタトゲチマダニの翻訳抑制遺伝子HI4EBPの特性解明

久米愛子, BOLDBAATAR Damdinsuren, 高澤木綿子, 白藤(梅宮)梨可, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　80th　84　2011年2月

ISSN： 1348-4613
フタトゲチマダニロンギシンP4のトキソプラズマに対する抗原虫作用

田仲哲也, 松尾智英, MORSHEDUR Rahman Md, BOLDBAATAR Damdinsuren, 白藤(梅宮)梨可, XUAN Xuenan, 辻尚利, 藤崎幸蔵

生化学　83回・33回　ROMBUNNO.4P-1168-1168　2010年12月
出版者・発行元： (公社)日本生化学会
ISSN： 0037-1017
フタトゲチマダニHaemaphysalis longicornis由来のクラスBスカベンジャー受容体CD36の同定と性状解析(Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis)

Aung Kyaw Min, Boldbaatar Damdinsuren, Liao Min, 白藤梨可, 宮, 中尾純大, 松岡輝重, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　150回　192-192　2010年9月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニのビテロジェニン合成におけるTOR経路の役割

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　150th　192-192　2010年9月1日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
ヒトデフェンシンのトキソプラズマに対する抗原虫作用

田仲哲也, M.・ラハマン, B, バッドゥル, D, ボルドバータル, 玄学南, 藤崎幸蔵

獣医寄生虫学会誌　9　(1)　66-66　2010年9月
出版者・発行元：日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニForkhead転写因子の同定と特性解明(Identification and characterization of Forkhead transcription factor from the hard tick, Haemaphysalis longicornis)

D.・ボルドバータル, 白藤梨可, 宮, 田仲哲也, 松岡輝重, 藤崎幸蔵

獣医寄生虫学会誌　9　(1)　72-72　2010年9月
出版者・発行元：日本獣医寄生虫学会
ISSN： 1347-961X
2 フタトゲチマダニにおけるリゾチームの発現動態(第59回日本衛生動物学会南日本支部大会講演要旨)

田仲哲也, 川野優, 中尾純大, 白藤(梅宮, 梨可, Rahman, Md. Morshedur, Battur Banzragch, Boldbaata Damdinsuren, 廖敏, 藤崎幸蔵

衞生動物　61　(2)　181-181　2010年6月15日
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086
3 Tick Cathepsin B-like Protease Involved in Embryonic Degradation of Vitellin(Proceedings of the 59th Annual Meeting of Southern Region)

Boldbaatar Damdinsuren, Battur Banzragch, 白藤(梅宮) 梨可, 廖敏, Rahman, Md. Morshedur, 田仲哲也, 藤崎幸蔵

衞生動物　61　(2)　181-181　2010年6月15日
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086
マダニカテプシンB様プロテアーゼはビテリンの胎生期分解と関係がある(Tick Cathepsin B-like Protease Involved in Embryonic Degradation of Vitellin)

Boldbaatar Damdinsuren, Battur Banzragch, 白藤梨可, 梅宮, 廖敏, Rahman, Md. Morshedur, 田仲哲也, 藤崎幸蔵

衛生動物　61　(2)　181-181　2010年6月
出版者・発行元：日本衛生動物学会
ISSN： 0424-7086
フタトゲチマダニにおけるセリン/スレオニンキナーゼAktの発現解析

田中徹, 白藤(梅宮)梨可, DAMDINSUREN Boldbaatar, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　79th　66　2010年5月

ISSN： 1348-4613
マダニ類(フタトゲチマダニ)由来のForkhead転写因子の同定と性状解析(Identification and characterization of Forkhead transcription factor from the hard tick, Haemaphysalis longicornis)

Damdinsuren Boldbaatar, 白藤梨可, 宮, 田仲哲也, 松岡輝重, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　149回　242-242　2010年3月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
マダニの遺伝子活性化におけるGATAタンパクの翻訳:フタトゲチマダニのGATA転写因子の同定

BOLDBAATAR Damdinsuren, BATTUR Banzragch, 白藤(梅宮)梨可, LIAO Min, 田仲哲也, 藤崎幸蔵

日本ダニ学会誌　18　(1)　39-40　2009年5月25日

ISSN： 0918-1067
フタトゲチマダニのディフェンシン様ペプチド(ロンギシン)の構造とその細胞溶解活性

RAHMAN Md.Morshedur, BOLDBAATAR Damdinsuren, LIAO Min, 白藤(梅宮)梨可, 辻尚利, 田仲哲也, 田原口智士, 藤崎幸蔵

日本ダニ学会誌　18　(1)　39　2009年5月25日

ISSN： 0918-1067
フタトゲチマダニにおけるリゾチームの性状解析

田仲哲也, 川野優, 中尾純大, 白藤(梅宮)梨可, MD.MORSHEDUR Rahman, BOLDBAATAR Damdinsuren, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　78th　89　2009年2月

ISSN： 1348-4613
フタトゲチマダニのオートファジー関連遺伝子の発現解析

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, LIAO Min, MD.MORSHEDUR Rahman, BATTUR Banzragch, 松尾智英, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　78th　86　2009年2月

ISSN： 1348-4613
フタトゲチマダニのFK506結合タンパクの同定および特性解明(一般講演,第16回日本ダニ学会大会講演要旨)

Boldbaatar Damdinsuren, Kilonzo Robert Musyoka, Battur Banzragch, 梅宮梨可, Liao Min, 田仲哲也, 玄学南, 藤崎幸蔵

日本ダニ学会誌　17　(1)　41-41　2008年5月25日
出版者・発行元：日本ダニ学会
ISSN： 0918-1067
フタトゲチマダニ由来新規オートファジー関連遺伝子の単離・同定および未吸血期における発現(一般講演,第16回日本ダニ学会大会講演要旨)

梅宮梨可, 松尾智英, 八田岳士, Boldbaatar Damdinsuren, 田仲哲也, 藤崎幸蔵

日本ダニ学会誌　17　(1)　41-42　2008年5月25日
出版者・発行元：日本ダニ学会
ISSN： 0918-1067
フタトゲチマダニのオートファジー関連遺伝子の単離・同定と中腸細胞におけるオートファゴソーム様構造体の観察

白藤(梅宮)梨可, 松尾智英, BOLDBAATAR Damdinsuren, LIAO Min, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　77th　41　2008年3月

ISSN： 1348-4613
フタトゲチマダニ由来の多発性ビトロジェニン遺伝子の同定とそのmRNA発現動態

Boldbaatar Damdinsuren, Battur Banzragch, 梅宮梨可, 廖敏, 田仲哲也, 玄学南, 藤崎幸蔵

獣医寄生虫学会誌　6　(2)　115-115　2008年3月
出版者・発行元：日本獣医寄生虫学会
ISSN： 1347-961X
Protein Disulfide Isomerases RNA干渉によるマダニHeamaphysalis longicornisの吸血と産卵に及ぼす影響

廖敏, 梅宮梨可, コン海燕, Harnnoi Thasaneeya, Boldbaatar Damdinsuren, 田仲哲也, 玄学南, 藤崎幸蔵

獣医寄生虫学会誌　6　(2)　116-116　2008年3月
出版者・発行元：日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニにおけるオートファジー関連遺伝子の発現動態と未吸血成ダニ中腸細胞における局在

梅宮梨可, 松尾智英, 八田岳士, D, ・ボルドバータル, 田仲哲也, 藤崎幸蔵

獣医寄生虫学会誌　6　(2)　123-123　2008年3月
出版者・発行元：日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニ由来の多発性ビテロジェニン遺伝子の同定とそのmRNA発現動態

DAMDINSUREN Boldbaatar, 梅宮梨可, BANZRAGCH Battur, 田仲哲也, 玄学南, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　144th　67-67　2007年8月27日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニにおけるオートファジー関連遺伝子の発現動態と未吸血成ダニ中腸細胞における局在

梅宮梨可, 松尾智英, 八田岳士, DAMDINSUREN Boldbaatar, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　144th　69-69　2007年8月27日
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
Comparison of activity and stability between native and recombinant bovine lactoperoxidase

S. Sato, T. Tanaka, K. Nakao, H. Kumura, K. Shimazaki

Australian Journal of Dairy Technology　58　209　2003年8月1日

ISSN： 0004-9433
トキソプラズマ感染防御におけるIFNγの役割

長沢秀行, 鎌田剛徳, 西川義文, 田仲哲也, 井上昇, 藤崎幸蔵, 見上彪, 鈴木直義

原生動物学雑誌　33　(1)　70　2000年3月21日

ISSN： 0388-3752
バキュロウイルスによるB.equiEMA-2の発現及び診断用抗原としての応用

田仲哲也, 玄学南, 筏井宏実, 五十嵐郁男, 長澤秀行, 藤崎幸蔵, 見上彪, 鈴木直義

日本獣医学会学術集会講演要旨集　128回　166-166　1999年9月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
バキュロウイルスによるB.equi EMA-1の発現と診断用抗原としての応用

玄学南, 五十嵐郁男, 筏井宏実, Avarzed A, 田仲哲也, 井上昇, 長澤秀行, 藤崎幸蔵, 豊田裕, 鈴木直義

日本獣医学会学術集会講演要旨集　126回　191-191　1998年8月
出版者・発行元： (公社)日本獣医学会
ISSN： 1347-8621
Structural and Immunochemical Studies of Bovine Anitmicrobial Peptide 'lactoferricin'

SHIMAZAKI Kei-ichi, NAM Myoung Soo, HARAKAWA Shinji, TANAKA Tetsuya, OMATA Yoshitaka, SAITO Atsushi, KUMURA Haruto, MIKAWA Katsuhiko, IGARASHI Ikuo, SUZUKI Naoyoshi

Peptide chemistry : proceedings of the ... Symposium on Peptide Chemistry　1996　(0)　197-200　1996年10月1日
抗ラクトフェリンモノクローナル抗体の結合する部位の推定

島崎敬一, 神尾真, NAM Myoung Soo, 玖村朗人, 三河勝彦, 原川信二, 田仲哲也, 小俣吉孝, 齋藤篤志, 五十嵐郁夫, 鈴木直義

日本分子生物学会年会プログラム・講演要旨集　19　(0)　399-399　1996年8月1日
ウシラクトフェリンに対するモノクローナル抗体について : 食品

神尾真, 南明〓, 島崎敬一, 玖村朗人, 三河勝彦, 原川信二, 田仲哲也, 小俣吉孝, 斎藤篤志, 五十嵐郁男, 鈴木直義

日本農藝化學會誌　70　(0)　2-2　1996年3月5日
出版者・発行元：社団法人日本農芸化学会
ISSN： 0002-1407
抗ラクトフェリシンモノクローナル抗体の作製とその性状 : 生体高分子・脂質

南明洙, 三河勝彦, 島崎敬一, 原川信二, 田仲哲也, 小俣吉孝, 斎藤篤志, 五十嵐郁男, 鈴木直義

日本農藝化學會誌　69　(0)　180-180　1995年7月5日
出版者・発行元：社団法人日本農芸化学会
ISSN： 0002-1407
抗ラクトフェリシン‐モノクローナル抗体の作成

原川信二, 田仲哲也, 小俣吉孝, 島崎敬一, 五十嵐郁男, 斎藤篤志, 鈴木直義

日本農芸化学会北海道支部・北海道農芸化学協会シンポジウム及び合同学術講演会講演要旨　1994　30　1994年

︎全件表示 ︎最初の5件までを表示

書籍等出版物 12

犬のライム病（動物の感染症<第五版>）

田仲哲也, 編集:迫田義博、秋庭正人、末吉益雄、高野友美、長井誠、芳賀猛、村瀬敏之

2025年
Ⅳ節足動物 1. ダニ類（動物寄生虫病学<四訂版>）

田仲哲也, 板垣匡, 藤﨑幸蔵

2019年
犬のライム病（動物の感染症<第四版>）

田仲哲也, 編集, 明石博臣, 内田郁夫, 大橋和彦, 後藤義孝, 須永藤子, 高井伸二, 宝達勉

2019年
"Ticks’ Antioxidant Complex: A Defense Stronghold and a Potential Target for Their Control" in Advances in Medicine and Biology Volume 116

Galay, R. L, Hernandez, E. P, Talactac, M. R, Kusakisako, K, Fujisaki, K, TANAKA Tetsuya, Editors, Leon V. Berhar

2017年
"RNA Interference: A Powerful Functional Analysis Tool for Studying Tick Biology Towards Tick and Pathogen Control" in RNA Interference

Galay, R. L, Umemiya-Shirafuji, R, Mochizuki, M, Fujisaki, K, TANAKA Tetsuya (Editors, Ibrokhim Y. Abdurakhmonov

2016年
"Host immunization with recombinant proteins to screen antigens for tick control" in Vaccine Design: Methods and Protocols

Galay, R. L, Miyata, T, Umemiya-Shirafuji, R, Mochizuki, M, Fujisaki, K, TANAKA Tetsuya (Editors, Sunil Thomas

2016年
第2章免疫の概念（獣医学教育モデル・コア・カリキュラム準拠獣医免疫学）

田仲哲也, 池田輝雄, 岡本まり子, 松本安喜, 編集, 池田輝雄, 小川健司, 松本安喜

2015年
第6章節足動物各論I（ダニ）（獣医学教育モデル・コア・カリキュラム準拠寄生虫病学）

田仲哲也, 編集, 堀井洋一郎, 野中成晃, 加藤大智, 平恵介, 松本淳, 山直明

2014年
"Tick lysozymes" in Microbial pathogens and strategies for combating them: science, technology and education

TANAKA Tetsuya, Fujisaki, K. (Editors, A. Mendez-Vilas

Formatex Research Center. Spain　2013年
"Expression and characterization of bovine milk antimicrobial proteins lactoperoxidase and lactoferrin by vaccinia virus" in Infectious Diseases in Grobal Scenario

TANAKA Tetsuya, Xuan X, Fujisaki, K, Shimazaki, K. (Editors, Priti Kumar Ro

InTech-Open Access Publisher. Croatia　2012年
"Antimicrobial activity of lactoferrin and lactoperoxidase in milk" in Dietary Protein Research Trends

TANAKA Tetsuya, Editors, Janet R. Ling

Nova Science Publishers, Inc. New York　2007年
3章牛乳とその成分の分析（乳肉卵の機能と利用）

島崎敬一, 田仲哲也, 編集, 阿久澤良造, 坂田亮一, 島崎敬一, 服部昭仁

（株）アイ・ケイコーポレーション　2005年

︎全件表示 ︎最初の5件までを表示

講演・口頭発表等 90

フタトゲチマダニ由来抗酸化蛋白質を発現する哺乳類細胞の発現安定株の作製

諸熊遥樹, 草木迫浩大, Talactac Melbourne R, Hernandez Emmanuel P, 田仲哲也

衛生動物　2019年6月
単為生殖系フタトゲチマダニの胚発生における抗酸化分子の発現プロファイルの作成

島崎慧, Hernandez Emmanuel Pacia, 新原博子, 藤崎幸蔵, 田仲哲也

衛生動物　2019年6月
Haemaphysalis longicornisグルタチオンSトランスフェラーゼ(HlGST2)はダニ幼生におけるクロルピリホス解毒に不可欠である(Haemaphysalis longicornis glutathione S-transferase(HlGST2) is vital in the detoxification of chlorpyrifos in larval ticks)

Hernandez Emmanuel, 草木迫浩大, Talactac Melbourne, Galay Remil, 八田岳士, 藤崎幸蔵, 辻尚利, 田仲哲也

日本獣医学会学術集会講演要旨集　2018年8月
哺乳類細胞におけるマダニアクチン遺伝子プロモーターを用いた外来遺伝子の発現

諸熊遥樹, 草木迫浩大, Talactac Melbourne R, Hernandez Emmanuel P, 田仲哲也

衛生動物　2018年6月
マダニ胚由来細胞における遺伝子発現ベクターの探索並びに構築

田仲哲也, 井戸明子, 草木迫浩大, 諸熊遥樹, 正谷達謄, Hernandez Emmanuel, Talactac Melbourne, 好井健太朗

生命科学系学会合同年次大会　2017年12月
フタトゲチマダニHaemaphysalis longicornisにおいて新規同定されたディフェンシン様ペプチドHEdefensinの性状解析と抗ウイルス活性(Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis)

Talactac Melbourne, 屋田友里花, 好井健太朗, Hernandez Emmanuel, 草木迫浩大, 前田大輝, 藤崎幸蔵, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　2017年8月
シカダニIxodes scapularis胚由来細胞株(ISE6)におけるFerritin 1発現誘導(Induction of Ferritin1 expression in Ixodes scapularis embryo derived cell line(ISE6))

Hernandez Emmanuel, 草木迫浩大, Galay Remil, Talactac Melbourne, 好井健太朗, 田仲哲也

日本獣医学会学術集会講演要旨集　2017年8月
マダニ-バベシア実験感染モデルの確立

前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林誠, 三好猛晴, 白藤梨可, 宮, 河津信一郎, 五十嵐郁男, 望月雅美, 辻尚利, 田仲哲也

日本獣医学会学術集会講演要旨集　2017年8月
マダニペルオキシレドキシンの特性解明(ペルオキシレドキシンの性状解析並びに吸血・産卵に果たす役割について)

草木迫浩大, 正谷達謄, 宮田健, Galay Remil, 白藤梨可, 宮, 前田大輝, Talactac Melbourne, Hernandez Emmanuel, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2017年8月
フタトゲチマダニヘモリンフ由来ディフェンシンの同定とその抗菌作用

屋田友里花, 草木迫浩大, Talactac Melbourne R, Hernandez Emmanuel P, 前田大輝, 安藤匡子, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　2017年7月
マダニ胚由来細胞における遺伝子発現ベクターの検証

井戸明子, 正谷達謄, 好井健太朗, Galay Remil L, 草木迫浩大, Talactac Melbourne R, Hernandez Emmanuel P, 望月雅美, 田仲哲也

衛生動物　2017年7月
Haemaphysalis longicornisにおけるThogotoウイルス(HI-Kamigamo-25)日本単離株の生存動態(Survival dynamics of Japan isolate Thogoto virus(HI-Kamigamo-25) in Haemaphysalis longicornis)

Talactac Melbourne, 好井健太朗, Hernandez Emmanuel, 草木迫浩大, 前田大輝, 藤崎幸蔵, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　2016年8月
マダニISE6細胞を用いた過酸化水素発生の可視化についての検討

草木迫浩大, Talactac Melbourne, Hernandez Emmanuel, 前田大輝, 好井健太朗, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　2016年8月
マダニHaemaphysalis longicornis由来の組換えグルタチオンSトランスフェラーゼの同定と性状解析(Identification and characterization of recombinant glutathione S- transferases from the hard tick Haemaphysalis longicornis)

Hernandez Emmanuel, 草木迫浩大, 前田大輝, Galay Remil, Talactac Melbourne, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2016年8月
鹿児島県各地で採取されたマダニにおけるマダニ媒介性原虫の分子疫学的解析

正谷達謄, 林慶, 立野守洋, 遠藤泰之, 松尾智英, 田仲哲也, 御供田睦代, 安藤匡子

日本獣医学会学術集会講演要旨集　2016年8月
Babesia ovata原虫におけるマダニ中腸形態へのステージシフト誘導実験

前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林誠, 白藤梨可, 梅宮, 辻尚利, 田仲哲也

日本獣医学会学術集会講演要旨集　2016年8月
マウス寄生性コクシジウムEimeria krijgsmanni感染の再活性化

水野真伸, 松林誠, 辻尾祐志, 正谷達謄, 田仲哲也, 松井利博, 松尾智英

日本獣医学会学術集会講演要旨集　2016年8月
鹿児島県屋久島のヒゲナガチマダニから分離されたリケッチアHKT-1株の遺伝子解析

安藤匡子, 本田俊郎, 御供田睦代, 藤田博己, 矢野泰弘, 正谷達謄, 田仲哲也, 松尾智英, 遠藤泰之, 安藤秀二, 川端寛樹

衛生動物　2016年4月
過酸化水素消去酵素2-Cysペルオキシレドキシンはマダニの吸血および産卵時の抗酸化機構に関与する

田仲哲也, 草木迫浩大, 正谷達謄, 宮田健, Galay Remil Linggatong, 白藤梨可, 宮, 前田大輝, Talactac Melbourne Rio, 辻尚利, 望月雅美, 藤崎幸蔵

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　2015年12月
ダニ媒介性脳炎ウイルスの代替としてのLangatウイルスに対するHaemaphysalis longicornisロンギシンP4ペプチドの殺ウイルス活性(Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus)

Talactac Melbourne Rio, 好井健太朗, 辻尚利, 藤崎幸蔵, 田仲哲也, 望月雅美

日本獣医学会学術集会講演要旨集　2015年8月
フタトゲチマダニにおけるペルオキシレドキシンの役割について

草木迫浩大, Linggatong, Galay Remil, 白藤梨可, 宮, 前田大輝, Talactac Melbourne Rio, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2015年8月
Toxoplasma gondii K-3株のシスト形成能に関する研究

小山田昇平, 正谷達謄, 辻尾祐志, 田仲哲也, 松井利博, 松尾智英

日本獣医学会学術集会講演要旨集　2015年8月
ベクターマダニ体内におけるBabesia ovata増殖動態についての一考察

前田大輝, 八田岳士, 松林誠, 三好猛晴, 白藤梨可, 宮, 大久保和洋, 五十嵐郁男, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2015年8月
フタトゲチマダニ由来TNF Receptor Associated Factor(TRAF)の同定とその役割について

武智理恵, Galay Remil Linggatong, 前田大輝, 草木迫浩大, 松尾智英, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2014年8月
フタトゲチマダニ由来2-Cys型ペルオキシレドキシンの機能解析

草木迫浩大, 正谷達謄, Galay Remil Linggatong, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2014年8月
マウス寄生性コクシジウムEimeria krijgsmanniの生活環に関する形態学的観察

小野結菜, 松林誠, 川口博明, 辻尾祐志, 田仲哲也, 三好宣彰, 松井利博, 松尾智英

日本獣医学会学術集会講演要旨集　2014年8月
フタトゲチマダニ(Haemaphysalis longicornis)におけるフェリチン類の重要な機能とダニ駆除の標的分子としての可能性(The critical functions of ferritins in Haemaphysalis longicornis and their potential as target molecules for tick control)

Galay Remil Linggatong, 白藤梨可, 宮, 宮田健, 草木迫浩大, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2014年8月
フタトゲチマダニ由来TNF Receptor Associated Factorの同定とその役割について

武智理恵, Galay Remil Linggatong, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　2014年6月
フタトゲチマダニ由来ロイシンリッチリピートドメイン保有蛋白質の動態と機能解析

栗巣孔士, 前田大輝, Galay Remil Linggatong, 武智理恵, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　2014年6月
犬と猫のウイルス病におけるダニ媒介性伝播の実験的考察

森寛行, 田仲哲也, 望月雅美

日本獣医学会学術集会講演要旨集　2013年8月
マウス腸管寄生性コクシジウムEimeriakrijgsmanniの薬剤感受性

竹尾俊規, 田仲哲也, 松林誠, 辻尾祐志, 橋本和樹, 辻尚利, 藤崎幸蔵, 松井利博, 松尾智英

日本獣医学会学術集会講演要旨集　2013年8月
フタトゲチマダニ体内におけるB.microtiの検出

草木迫浩大, 前田大輝, Galay Remil Linggatong, 松尾智英, 白藤梨可, 宮, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2013年8月
フタトゲチマダニ由来C型レクチン様蛋白質の同定とその特性解明

前田大輝, 草木迫浩大, Galay Remil Linggatong, 白藤梨可, 宮, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2013年8月
フタトゲチマダニHaemaphysalis longicornisにおいてフェリチンは鉄介在性酸化ストレスを防御する(Ferritins protect the hard tick Haemaphysalis longicornis from iron-mediated oxidative stress)

Galay Remil Linggatong, 草木迫浩大, 前田大輝, 白藤梨可, 梅宮, Tan Bacolod Eugene, 小山次郎, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2013年8月
フタトゲチマダニにおけるウサギ血液由来トランスフェリンの動態

森寛行, ガライ・レミル・リングガートン, 前田大輝, 松尾智英, 白藤梨可, 宮, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　2013年6月
フタトゲチマダニロンギシンにおける抗バベシア活性を持つペプチド部位の検索

田仲哲也, Galay Remil Linggatong, 前田大輝, Aung Kyaw Min, 白藤梨可, 梅宮, 玄学南, 五十嵐郁男, 望月雅美, 辻尚利, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2013年3月
マダニの卵黄タンパク質前駆体合成を制御する分子機構の解明

白藤梨可, 梅宮, Boldbaatar Damdinsuren, Liao Min, Battur Banzragch, Rahman Morshedur Md, Kuboki Thasaneeya, Galay Remil Linggatong, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2013年3月
フタトゲチマダニの鉄依存性酸化ストレスから保護するフェリチンの重要性

田仲哲也, GALAY Remil L, 白藤(梅宮)梨可, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵

日本分子生物学会年会プログラム・要旨集(Web)　2013年
フタトゲチマダニ卵黄タンパク質前駆体合成におけるTOR阻害剤ラパマイシンの影響

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2012年8月31日
フタトゲチマダニ由来サイクロフィリンAとバベシア原虫の関連性

前田大輝, DAMDINSUREN Boldbaatar, 草木迫浩大, KYAW MIN Aung, GALAY Remil, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2012年8月31日
カタダニHaemaphysalis longicornisの吸血と生殖の遂行における鉄蓄積タンパク質であるフェリチンの重要性(The importance of iron storage protein ferritin on successfu blood feeding and reproduction of the hard tick Haemaphysalis longicornis)

Galay Remil, Aung Kyaw Min, 白藤梨可, 宮, 前田大輝, 松尾智英, 川口博明, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2012年8月
フタトゲチマダニ由来サイクロフィリンAの抗バベシア原虫作用

前田大輝, 田仲哲也, BOLDBAATAR D, 草木迫浩大, AUNG KM, GALAY RL, 白藤(梅宮)梨可, 藤崎幸蔵

衛生動物　2012年6月30日
微生物貪食におけるフタトゲチマダニクラスBスカベンジャー受容体の重要性

キャウ・ミン・アウン, ダミディスレン・ボルドバータル, 白藤梨可, 宮, ガライ・レミル・リンガートン, 久米愛子, 田仲哲也, 藤崎幸蔵

衛生動物　2012年6月
フタトゲチマダニにおける宿主血液由来トランスフェリンの動態について

田仲哲也, 森寛行, GALAY Remil Linggatong, 前田大輝, 白藤(梅宮)梨可, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2012年3月1日
マダニのオートファジー関連遺伝子とその役割

白藤(梅宮)梨可, 川野優, BOLDBAATAR Damdinsuren, 松尾智英, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2012年3月1日
フタトゲチマダニの吸血および産卵におけるフェリチンの重要性

GALAY Remil L, KYAW MIN Aung, 白藤(梅宮)梨可, 前田大輝, 松尾智英, 川口博明, 三好宣彰, 鈴木宏志, XUAN Xuenan, 望月雅美, 藤崎幸蔵, 田仲哲也

日本分子生物学会年会プログラム・要旨集(Web)　2012年
フタトゲチマダニにおけるオートファジー関連遺伝子HlATG6発現抑制の産卵への影響

川野優, 白藤(梅宮)梨可, BOLDOBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　2011年6月15日
TOR経路はフタトゲチマダニのビテロジェニン合成に必須である.

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　2011年6月15日
フタトゲチマダニにおけるセリン/スレオニンキナーゼAktの発現及び機能解析

田中徹, 白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　2011年6月15日
フタトゲチマダニにおけるクラスBスカベンジャー受容体CD36の機能解析

Aung Kyaw Min, Boldobaatar Damdinsuren, 白藤梨可, 宮, 田仲哲也, 藤崎幸蔵

衛生動物　2011年6月
フタトゲチマダニロンギシンのトキソプラズマ原虫に及ぼす影響について

田仲哲也, 前田大輝, 松尾智英, Damdinsuren Boldbaatar, 白藤梨可, 梅宮, 玄学南, 辻尚利, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2011年3月
マダニにおけるオートファジーの役割

白藤梨可, 梅宮, 松尾智英, 川野優, Boldbaatar Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2011年3月
フタトゲチマダニロンギシンP4のトキソプラズマに対する抗原虫作用

田仲哲也, 松尾智英, Morshedur Rahman Md, Boldbaatar Damdinsuren, 白藤梨可, 梅宮, 玄学南, 辻尚利, 藤崎幸蔵

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　2010年12月
フタトゲチマダニにおけるオートファジー関連遺伝子HIATG6の機能解析

川野優, 白藤梨可, 梅宮, Boldbaatar Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2010年9月
フタトゲチマダニHaemaphysalis longicornis由来のクラスBスカベンジャー受容体CD36の同定と性状解析(Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis)

Aung Kyaw Min, Boldbaatar Damdinsuren, Liao Min, 白藤梨可, 宮, 中尾純大, 松岡輝重, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2010年9月
フタトゲチマダニのビテロジェニン合成におけるTOR経路の役割

白藤梨可, 梅宮, Boldbaatar Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2010年9月
3 Tick Cathepsin B-like Protease Involved in Embryonic Degradation of Vitellin(Proceedings of the 59th Annual Meeting of Southern Region)

Boldbaatar Damdinsuren, Battur Banzragch, 白藤(梅宮) 梨可, 廖敏, Rahman, Md. Morshedur, 田仲哲也, 藤崎幸蔵

衞生動物　2010年6月15日
2 フタトゲチマダニにおけるリゾチームの発現動態(第59回日本衛生動物学会南日本支部大会講演要旨)

田仲哲也, 川野優, 中尾純大, 白藤(梅宮, 梨可, Rahman, Md. Morshedur, Battur Banzragch, Boldbaata Damdinsuren, 廖敏, 藤崎幸蔵

衞生動物　2010年6月15日
マダニカテプシンB様プロテアーゼはビテリンの胎生期分解と関係がある(Tick Cathepsin B-like Protease Involved in Embryonic Degradation of Vitellin)

Boldbaatar Damdinsuren, Battur Banzragch, 白藤梨可, 梅宮, 廖敏, Rahman, Md. Morshedur, 田仲哲也, 藤崎幸蔵

衛生動物　2010年6月
フタトゲチマダニにおけるリゾチームの発現動態

田仲哲也, 川野優, 中尾純大, 白藤梨可, 梅宮, Rahman, Md. Morshedur, Battur Banzragch, Boldbaata Damdinsuren, 廖敏, 藤崎幸蔵

衛生動物　2010年6月
マダニ類(フタトゲチマダニ)由来のForkhead転写因子の同定と性状解析(Identification and characterization of Forkhead transcription factor from the hard tick, Haemaphysalis longicornis)

Damdinsuren Boldbaatar, 白藤梨可, 宮, 田仲哲也, 松岡輝重, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2010年3月
ヒトデフェンシンのトキソプラズマに対する抗原虫作用

田仲哲也, Morshedur Rahman Md, Banzragch Battur, Damdinsuren Boldbaatar, 玄学南, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2010年3月
フタトゲチマダニ由来新規オートファジー関連遺伝子の単離・同定および未吸血期における発現(一般講演,第16回日本ダニ学会大会講演要旨)

梅宮梨可, 松尾智英, 八田岳士, Boldbaatar Damdinsuren, 田仲哲也, 藤崎幸蔵

日本ダニ学会誌　2008年5月25日
フタトゲチマダニのFK506結合タンパクの同定および特性解明(一般講演,第16回日本ダニ学会大会講演要旨)

Boldbaatar Damdinsuren, Kilonzo Robert Musyoka, Battur Banzragch, 梅宮梨可, Liao Min, 田仲哲也, 玄学南, 藤崎幸蔵

日本ダニ学会誌　2008年5月25日
ハウスダストマイトアレルゲン由来ProDerp 3の低アレルゲン性

田仲哲也, Magi Mauro, Vandenbranden Michel, 藤崎幸蔵, Jacquet Alain

日本獣医学会学術集会講演要旨集　2007年8月
フタトゲチマダニにおけるオートファジー関連遺伝子の発現動態と未吸血成ダニ中腸細胞における局在

梅宮梨可, 松尾智英, 八田岳士, Damdinsuren Boldbaatar, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2007年8月
フタトゲチマダニ由来の多発性ビテロジェニン遺伝子の同定とそのmRNA発現動態

Damdinsuren Boldbaatar, 梅宮梨可, Banzragch Battur, 田仲哲也, 玄学南, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2007年8月
牛乳由来オステオポンチンのキモシンに対する感受性

三浦敦史, 玖村朗人, 佐藤恵理子, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2004年3月
ラクトフェリンのLactobacillus acidophilusに対する増殖促進効果について

大橋美登里, 金完燮, 田仲哲也, 玖村朗人, 島崎敬一

日本畜産学会大会講演要旨集　2003年9月
ラクトペルオキシダーゼのメナジオン誘導細胞死に及ぼす影響

石川稿太郎, 田仲哲也, 玖村朗人, 島崎敬一

日本畜産学会大会講演要旨集　2003年9月
プロバイオティクス効果を示す酵母の探索および機能解析

田上結子, 塚原真己, 玖村朗人, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2003年9月
ウシラクトフェリンのイヌヘルペスウイルスに対する抗ウイルス作用

田仲哲也, 中谷彰吾, 玄学南, 玖村朗人, 五十嵐郁男, 島崎敬一

日本獣医学会学術集会講演要旨集　2003年3月
ラット乳線組織におけるpolymeric immunoglobulin receptor(pIgR)の発現に関する研究

飽田恵介, 玖村朗人, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2003年3月
経口投与したウシラクトフェリンのラットにおける体内動態

吉瀬蘭エミリー, 田仲哲也, 松本恵, 玖村朗人, 桑田英文, 知地英征, 島崎敬一

日本栄養・食糧学会大会講演要旨集　2002年6月
ラットにおける経口投与したウシラクトフェリン及び同フラグンメントの体内動態

吉瀬蘭エミリー, 田仲哲也, 松本恵, 玖村朗人, 知地英征, 島崎敬一

日本畜産学会大会講演要旨集　2002年3月
Trypanosoma bruceiのウシラクトフェリン結合タンパク質の検出

田仲哲也, 阿部雪絵, 井上昇, 玖村朗人, 長澤秀行, 島崎敬一

日本獣医学会学術集会講演要旨集　2002年3月
ラット乳線上皮細胞におけるpolymeric immunoglobulin receptor(pIgR)発現の定量的検出

飽田恵介, 玖村朗人, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2001年8月
ラットα-カゼインのN末端領域ペプチドの分離

高垣謙太郎, 玖村朗人, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2001年8月
乳腺上皮細胞におけるpolymeric immunoglobulin receptor(pIgR)の発現因子に関する研究

田中厚志, 玖村朗人, 青塚雅基, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2001年3月
二次元電気泳動及びTOF-MSを用いたラット乳腺組織中のタンパク質の分析及び同定

高垣謙太郎, 玖村朗人, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2001年3月
IFN-γにおけるNeospora caninum増殖抑制効果マウス腹腔マクロファージにおける一酸化窒素依存性経路の役割について

田仲哲也, 長澤秀行, 五十嵐郁男, 藤崎幸蔵, 鈴木直義, 見上彪

日本獣医学会学術集会講演要旨集　2000年3月
バキュロウイルスによるB.equiEMA-2の発現及び診断用抗原としての応用

田仲哲也, 玄学南, 筏井宏実, 五十嵐郁男, 長澤秀行, 藤崎幸蔵, 見上彪, 鈴木直義

日本獣医学会学術集会講演要旨集　1999年9月
Neospora caninum感染BALB/cマウスにおけるCD4+及びCD8+T細胞の役割(T Cell Responses in SAG-1 Transgenic Mice to T.gondii infection)

浜田岳人, 田仲哲也, 井上昇, 長澤秀行, 藤崎幸蔵, 見上彪, 鈴木直義

日本獣医学会学術集会講演要旨集　1999年9月
T.gondii主要表面タンパク質P30を発現する組換えネコヘルペスウイルスの作製

玄学南, 三島雅之, 田仲哲也, 西川義文, 五十嵐郁男, 長澤秀行, 藤崎幸蔵, 鈴木直義, 見上彪

日本獣医学会学術集会講演要旨集　1999年3月
組換えToxoplasma gondii P24の発現

田仲哲也, 玄学南, 井上昇, 加藤美穂子, 五十嵐郁男, 長澤秀行, 藤崎幸蔵, 見上彪, 鈴木直義

日本獣医学会学術集会講演要旨集　1999年3月
バキュロウイルスによるB.equi EMA-1の発現と診断用抗原としての応用

玄学南, 五十嵐郁男, 筏井宏実, Avarzed A, 田仲哲也, 井上昇, 長澤秀行, 藤崎幸蔵, 豊田裕, 鈴木直義

日本獣医学会学術集会講演要旨集　1998年8月
抗ラクトフェリンモノクローナル抗体の結合する部位の推定

島崎敬一, 神尾真, NAM Myoung Soo, 玖村朗人, 三河勝彦, 原川信二, 田仲哲也, 小俣吉孝, 齋藤篤志, 五十嵐郁夫, 鈴木直義

日本分子生物学会年会プログラム・講演要旨集　1996年8月1日
ウシラクトフェリン並びにウシラクトフェリシン投与マウスにおけるToxoplasma gondii感染に対する防御効果

田仲哲也

日本獣医学会学術集会講演要旨集　1996年4月
ウシラクトフェリンに対するモノクローナル抗体について : 食品

神尾真, 南明〓, 島崎敬一, 玖村朗人, 三河勝彦, 原川信二, 田仲哲也, 小俣吉孝, 斎藤篤志, 五十嵐郁男, 鈴木直義

日本農藝化學會誌　1996年3月5日
抗ラクトフェリシンモノクローナル抗体の作製とその性状 : 生体高分子・脂質

南明洙, 三河勝彦, 島崎敬一, 原川信二, 田仲哲也, 小俣吉孝, 斎藤篤志, 五十嵐郁男, 鈴木直義

日本農藝化學會誌　1995年7月5日

︎全件表示 ︎最初の5件までを表示

産業財産権 4

マラリア原虫増殖阻害用組成物

宮田健, 田仲哲也, 大林桃百香

産業財産権の種類: 特許権
抗微生物ポリペプチド

田仲哲也, メルボルンリオ, タラクタック, 藤崎幸蔵

産業財産権の種類: 特許権
抗マダニワクチン

田仲哲也, ガライレミル, リンガートン, 藤崎幸蔵

産業財産権の種類: 特許権
デキストラン分解酵素

島崎敬一, 玖村朗人, 田仲哲也

産業財産権の種類: 特許権

共同研究・競争的資金等の研究課題 27

ウイルス感染マダニを用いたウイルス感染防御における抗酸化分子の役割解明

田仲哲也, 好井健太朗

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research

研究種目：Grant-in-Aid for Scientific Research (B)

研究機関：Tohoku University

2025年4月1日～ 2029年3月31日
RPAとCRISPR/Cas12aを組み合わせたマダニ媒介性感染症の簡易迅速的な診断法の開発

田仲哲也, 一二三達郎

2025年6月～ 2028年3月
東南アジアにおけるマダニ媒介性動物感染症の実態解明と簡易迅速的な診断法の構築

田仲哲也, 山崎渉

2024年4月～ 2026年3月
多分野融合による節足動物媒介性ウイルスの宿主間伝播・病態形成機構の解明と治療法創出に関する研究開発競争的資金

好井健太朗, 小林純子, 平野港, 田仲哲也, 椎森仁美

2023年6月～ 2026年3月
二本鎖RNAバイオナノカプセルを用いた抗酸化分子を標的とするRNA殺ダニ剤の開発

田仲哲也, 岡村勝友

2022年4月1日～ 2025年3月31日
二本鎖RNAバイオナノカプセルを用いた抗酸化分子を標的とするRNA殺ダニ剤の開発

田仲哲也, 岡村勝友

2022年4月～ 2025年3月

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マダニの生存基盤が宿主動物からの吸血・消化にあり、血液消化産生物中に含まれるヘム代謝の過程で、大量の鉄や過酸化物がマダニ体内に放出される可能性が考えられる。鉄や過酸化物から被る酸化ストレスの制御は、吸血・産卵、また宿主への寄生適応の成否を左右する重要な機構である。研究代表者らはその重要な機構を担う抗酸化分子として、フェリチン(FER)、ペルオキシレドキシン(PRX)、グルタチオンSトランスフェラーゼ(GST)遺伝子を同定した。本研究では、それらを標的とした二本鎖(ds)RNAバイオナノカプセルを用いたRNA殺ダニ剤を開発することを最終目標とする。
ホロゲノムトランスクリプトーム解析を用いてマダニと細菌の共生関係の分子基盤に迫る

林哲也, 田仲哲也

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

研究種目：Grant-in-Aid for Challenging Research (Exploratory)

研究機関：Kyushu University

2021年7月9日～ 2024年3月31日

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リケッチアは経卵感染が可能なためマダニと緊密な共生関係にあるが、その相互作用は明らかになっていない。紅斑熱リケッチアのホスト/ベクターであるフタトゲチマダニH. longicornis (LON)には両性生殖系と単為生殖系が存在し、多くの両性系株にはR. longicornii (Rlon)が存在し、経卵感染で安定に維持されている。本研究では10年以上にわたって継代されているLONの飼育系を利用して、Rlon保有系と非保有系を確立し、Rlon共生によるマダニの特性変化を解析するとともに、発育ステージの進行に沿ったホロトランスクリプトーム解析を行い、マダニでのリケッチア遺伝子の発現と各ステージおけるマダニ遺伝子の発現を解析し、LONとRlonの共生関係の実態と関連遺伝子群の同定すること、さらにこれをモデル系としてマダニと病原体の共生関係の実態解明という研究分野の開拓を目指す。本年度は、技術基盤となる２項目に関して解析を進めた。 1. Rlon保有系統の確立：Rlonの両性・単為生殖系LONへの感染実験を行い、接種検体の調整法の違いによるRlonの感染効率と安定性を検討した。具体的には、３通りの方法で調整したRlon検体を血体腔接種によりLON個体に接種し、LON個体内のRlonコピー数をqPCRにより解析した。現在、再現性の確認を行なっている所であるが、少なくとも4週間後までは、Rlonが両系統に保持されることを確認でき、最も効率の良い接種検体調整法を決定した。 2. ホロゲノムトランスクリプトーム解析系の確立：鍵となるのはrRNAの除去であるため、まず最近利用可能となったQIAseq社のキットを用いて、マウスと細菌（サンプル調整の問題から別菌種を使用）の混合検体からのrRNA除去効率を検討し、マウス培養細胞への感染実験（コントロール実験）に対しては対応可能であることを確認した。
フィリピンにおける牛マダニ媒介性感染症のゲノム疫学調査と実践的診断法の構築

田仲哲也

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research

研究種目：Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

研究機関：Kagoshima University

2020年10月27日～ 2024年3月31日

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マダニ媒介性感染症は、人や家畜に致死的な症状を引き起こし、地球規模で畜産業の脅威となっている。マダニ媒介性感染症の制御には、流行実態をグローバル(空間的)な視点により解明し、その地で現在流行しているマダニ媒介性感染症に適した「現地即応型」な診断対策を講じる必要がある。本研究では、我が国と畜産・農産品の輸入貿易が盛んなフィリピンに着目した。当国における遺伝的に多様なマダニ媒介性病原体の集団を対象とし、ゲノム疫学的手法に基づくマダニ媒介性感染症の流行実態の解明と、ゲノム情報に立脚した現地即応型のマダニ媒介性感染症における診断法の構築を目指す。本成果は畜産業向上のための「国際貢献」につながる。本共同研究はフィリピンにおける牛マダニ媒介性感染症の流行実態を明らかにし、フィリピンの診断対策の向上に資することを最終目標とした。しかし、COVID-19影響のため、フィリピンに行くことができず、鹿児島県で採取した牛の血液を用いて、以下の実験を行った。・鹿児島県における牛マダニ媒介性感染症の疫学調査：広範囲の疫学調査と主要流行マダニ媒介性病原体の特定（202１-2022年）【国内ラボワーク主担当：田仲・山崎・草木迫】 ① 遺伝子診断法の構築：牛ピロプラズマ症について、現地で利用し易いLAMPやRPA解析用のプライマーを考案し、LAMP法やRPA法を確立した。 ② DNAサンプル解析：2018年に採取した牛の血液DNAサンプルを用いて、牛ピロプラズマ症の遺伝子診断・解析を行った。
マダニベクターの病原体伝播における唾液アシストメカニズムの解析

八田岳士, 田仲哲也, 藤田修

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

研究種目：Grant-in-Aid for Scientific Research (B)

研究機関：Kitasato University

2020年4月1日～ 2024年3月31日

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ヒトからヒトまたは動物からヒトに感染症を伝播する能力をもつベクターは、吸血時に宿主体内へ注入する唾液が宿主免疫系を抑制的に制御し病原体伝播を促進する。研究代表者は、ベクターの中でも蚊に次いで多種多様な病原体を媒介するマダニの唾液分子ロンギスタチンに、皮膚炎症抑制効果が実際に存在することを示してきたが、SAT現象の存否については解明できていない。そこで、マダニ刺咬部の微小環境における唾液・宿主免疫系・病原体の三者間のインタラクトームに着目し、宿主免疫系に作用する唾液分子について、in vitroの免疫細胞実験や遺伝子抑制マダニを使ったin vivo解析による分子機能解析を通して網羅的に同定する。ついでマダニ媒介感染症マウスモデルにて、マダニからの伝播試験を実施し、唾液分子におけるSAT効果の有無について検討し「マダニSAT理論」の構築を目指す。本年度では、吸血時間軸に沿った宿主皮下微小環境の病理組織解析と唾液腺トランスクリプトーム解析を並行し、マダニが宿主皮膚に形成するBlood Pool（BP）や病原体伝播に関与する唾液分子の機能解明に着手した。病理組織解析の結果、BPでは、吸血開始後有核細胞の集積を経て赤血球が真皮内に浸潤し始め、飽血間際には皮下結合組織にも赤血球浸潤領域が拡大している様子を観察したが、血腫様構造が認められなかった。さらに解析を進めたところBP形成のトリガーである皮下微小血管の破綻が、トランスクリプトーム解析によって見出した唾液分子HlSG-g22により惹起されていると考えられる知見を得た。HlSG-g22の分子構造モデルが、血管内皮細胞接着分子VE-Cadherinの発現を促進するケモカインCXCL1の阻害分子Evasin-3であったことから、マダニにとって効率よく吸血するためには、炎症細胞が分泌するCXCL1を強固に抑制する必要があるのかもしれない。
新しい遺伝子検出技術を用いたマダニ媒介性動物感染症の簡易・迅速診断方法の開発

田仲哲也

2021年～ 2021年

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マダニは家畜に甚大な被害を与えているリケッチア症やピロプラズマ症などの多種多様な病原体の伝播に関与し得る、優れた疾病媒介能を有している。このため、マダニとマダニ媒介性動物感染症が世界経済に与える脅威は甚大であり、マダニおよびマダニ媒介性疾病対策は公衆衛生や畜産分野においては不可欠である。このように、マダニ防除のためのワクチン・治療薬開発に繋がる研究に極めて重要な情報をもたらすものとして、マダニ媒介性疾病を公衆衛生学的に評価する疫学的研究が必要とされている。そこで、我々はマダニ媒介性病原体をフィールドでの特殊な整備などを必要としない簡易・迅速的な検出技術を開発することを本研究の目的とした。
病原体伝播を阻止するマダニの遺伝子改変ドライブの開発

田仲哲也

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research

研究種目：Grant-in-Aid for Challenging Research (Exploratory)

研究機関：Kagoshima University

2017年6月30日～ 2020年3月31日

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[全体構想] 近年標的配列選択の自由度が高く、作製の簡便なCRISPR/Cas9ゲノム編集システムが開発され、これまで標的遺伝子の改変が困難であった生物でのゲノム編集が可能となった。しかし、マダニ遺伝子機能の解析ではRNA干渉法による遺伝子破壊が用いられてきたが、ノックアウト技術は確立できていない。そこで、研究代表者はCRISPR/Cas9システムに着目し、マダニの感染防御に関わる遺伝子ノックアウト技術の確立を試み、これらを用いた感染症モデルを作製することを本研究の主要目的とする。 [今まで得られた研究実績]マダニのウイルス感染防御に関わる遺伝子を同定するために、ウイルス感染防御に関わることが予想されるディフェンシン、スカベンジャーレセプター遺伝子を標的として、これらの遺伝子をノックダウン後、ウイルス感染実験を行った。これらのマダニにランガットウイルスを肛門接種によって感染させた後、マダニの生存率やマダニのウイルス感染量を調べることによって、ディフェンシン、スカベンジャーレセプター遺伝子の感染防御の役割について検討したが、マダニの生存率やウイルス感染量はノックダウン未処置群と比べて、大きな差は認められなかった。次に、遺伝子操作系を確立する上で必要となる、マダニ細胞内で外来遺伝子を高発現する遺伝子発現ベクターの探索並びに構築した。その結果、マダニアクチン遺伝子の推定プロモーター配列に引き続きマダニフェリチン遺伝子の推定プロモーター配列を決定することに成功し、その活性部位も同定した。このプロモーターは鉄濃度によってスイッチをオン・オフすることが可能であり、マダニ遺伝子操作系の確立において、鉄濃度依存による遺伝子発現ベクターとして使用できることが分かった。
ウイルス由来ノンコーディングRNAによるフラビウイルス感染制御メカニズムの解明

好井健太朗, 田仲哲也, 小林進太郎

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research

研究種目：Grant-in-Aid for Scientific Research (B)

2017年4月1日～ 2020年3月31日

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本研究においては、ダニ媒介性フラビウイルスであるダニ媒介性脳炎ウイルス（TBEV）について、これまでの研究成果で推定された、ウイルス遺伝子RNA由来長鎖ノンコーディングRNA（lncRNA）の産生に関わると推定される領域に変異を導入したウイルスを作製し、その性状を解析した。ウイルスを感染させ、細胞内におけるウイルス由来lncRNAの産生状況について解析した所、哺乳動物細胞と節足動物由来培養細胞で産生されるlncRNAのパターンに相違があることが明らかになった。また、哺乳動物細胞においては変異による増殖性の相違は認められなかったが、節足動物細胞においては増殖の遅延が認められ、特定のウイルス由来lncRNAが、節足動物における効率の良い増殖に関与している可能性が示唆された。またマウスモデルにウイルスを感染させ、病原性を検討した所、変異により接種時の病原性の上昇が認められた。さらにレポーターアッセイにより、ウイルス感染細胞中のインターフェロン応答を解析した所、ウイルス感染によりインターフェロンプロモーター活性の抑制が認められたが、変異によりこの抑制が増強されることが示された。これらの事よりウイルス由来lncRNAはマウス体内において、自然免疫応答の制御機構があり、これが病態発現につながっている可能性が示唆される。以上の成果から、ウイルス由来lncRNAは感染宿主の種に応じて異なる機能を持っており、これらが自然界におけるウイルス伝播や、病態発現に関与していると考えられるため、今後詳細に解析していく必要があると考えられる。
マダニ制圧へ向けたマダニの抗酸化分子を標的とするワクチンとRNA殺ダニ剤の創出

田仲哲也, 黒田俊一, 宮田健

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research

研究種目：Grant-in-Aid for Scientific Research (B)

研究機関：Kagoshima University

2016年4月1日～ 2019年3月31日

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[全体構想] マダニにとって必須の生存基盤が宿主動物からの吸血・消化にあり、血液消化産物中に含まれるヘム代謝の過程で多量の鉄や過酸化物がマダニ体内に放出する可能性が考えられる。鉄や過酸化物から被る酸化ストレスへの応答、さらには酸化還元バランスのコントロールは、吸血や産卵、また宿主への寄生適応の成否を左右する重要な機構である。その詳細については解明されていないが、研究代表者らはその重要な機構を担う抗酸化分子として、フェリチン、ペルオキシレドキシン、グルタチオンSトランスフェラーゼ遺伝子を同定することに成功した。本研究では、マダニの酸化還元バランス制御機構の特性を明らかにし、マダニ制圧開発研究の場に新知見を提供すると同時に、それらを標的とした抗マダニワクチンとRNA殺ダニ剤を創出することを目標とする。 [今まで得られた研究実績] マダニペルオキシレドキシン (Prx2) は、マダニの吸血・産卵において過酸化水素濃度の制御に重要な分子であった。このため、組換えPrx2で免疫した宿主動物でマダニを吸血させ、マダニの吸血並びに産卵に及ぼす影響を評価し、組換えPrx2 がワクチン標的候補分子となるか検証した。ウサギ並びにマウスにおいて、組換えPrx2 のみ免疫でも十分な抗体価を誘導でき、宿主体内にできた特異的抗体が、マダニ吸血に反応した。しかし、実際に免疫ウサギで雌成ダニを吸血させたところ、吸血並びに産卵への顕著な影響は認められず、免疫マウスで若ダニを吸血させても、若ダニの吸血並びにその発育への顕著な影響は認められなかった。これらの結果成果から、宿主への組換えPrx2 免疫はマダニの吸血、生育、産卵に大きな影響を及ぼさないことが示唆された。しかし、rHlPrx2 のみの免疫でも宿主に十分な抗体価を誘導できたことから、組換えPrx2 は生物由来アジュバントとしての応用の可能性が考えられた。
フィリピンにおける牛のマダニ媒介性動物感染症の実態解明と診断・予防法の確立

田仲哲也, 安藤匡子, 正谷達謄

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

研究種目：Grant-in-Aid for Scientific Research (B)

研究機関：Kagoshima University

2015年4月1日～ 2019年3月31日

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牛のアナプラズマ症やバベシア症含むマダニ媒介性感染症は、世界規模で甚大な被害を与えており、海外悪性伝染病に指定されている。本研究は、新たな診断法を開発すると共に、フィリピンにおける牛のアナプラズマ症ならびにバベシア症の感染状況を明らかにし、日本のアナプラズマ症とバベシア病に対する検疫体制強化に貢献することを目的として行われた。その結果、牛のアナプラズマ症ならびにバベシア症に対する新規の遺伝子診断法が開発された。これらの診断法を用いたフィリピンマニラ周辺地域における牛のアナプラズマ症ならびにバベシア症の疫学調査により、これら病原体を含むマダニ媒介性病原体の高い感染率が認められた。
病原体媒介節足動物の共生微生物ライブラリーの構築と病原体制御への応用

中尾亮, 阿部貴志, 田仲哲也

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)

研究種目：Grant-in-Aid for Young Scientists (A)

研究機関：Hokkaido University

2015年4月1日～ 2019年3月31日

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マダニなどの節足動物は、病気を引き起こす病原体を体内に保有して、人や動物に伝播する。本研究では、そのような病原体媒介節足動物が他にどのような微生物の集団を保有するかを明らかにすることを目的とした。国内外で採集されたマダニを材料に、微生物由来の遺伝子を解析したところ、コクシエラやリケッチア等の特定の細菌群が優占して存在することが分かった。マダニとそれら優占細菌群の遺伝子型を比較したところ、親から子へ垂直伝播するものと、個体間で水平伝播するものの両方の存在が示された。
マダニの低酸素応答機構の解明と抗マダニ薬の創出

辻尚利, 八田岳士, 田仲哲也, 白藤梨可

2013年5月31日～ 2017年3月31日

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これまでに取り組んできた『マダニの吸血と病原体伝播の分子基盤の解明』から、抗マダニ薬を開発する創薬プラットフォームの構築において、創薬分子の標的となる『吸血・病原体伝播調節物質』が低酸素応答によって一括制御されていることを受けていることが判明している。開始３年目の本年度は、これまでに明らかにされたマダニにおける低酸素応答経路と吸血行動を支える代謝系経路の相互作用の知見をもとに、バベシア原虫感染マダニにおける原虫と低酸素応答経路の相互関係の解析を実施した。はじめに、マダニ個体内におけるバベシア原虫の分化・増殖はマダニ代謝に依存することが分かってきていることを受け、低酸素応答機構と原虫生存に与える影響を調べ、細胞内外、臓器間に構築されたネットワークと、病原体媒介の相互関係の動的変化を病原体伝播システムとして捉えた統括的理解を図った。これによって、①原虫感染によるHR調節物質の阻害・制御の動態及びHR調節物質が原虫の発育・伝播に果たす機能を細胞、組織、臓器別に明らかとなった。②各種阻害剤を用いたキナーゼアッセイを行った結果、マダニ個体における原虫伝播時の役割を明らかにすることができた。③原虫感染が解糖系HIF-1、PI-3K/AKT/TOR経路に及ぼす影響を調べた結果、原虫感染による低酸素応答経路に変動があることが分かった。④さらに、分子間クロストークを介した細胞自律的な原虫制御機構としての低酸素応答経路の機能と役割解明から、マダニ媒介性病原体の発育・伝播を支配する分子基盤が存在することが明らかとなった。
Toll情報伝達経路を標的とした新規のマダニ防圧法開発

田仲哲也, 松尾智英, 辻尚利

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

研究種目：Grant-in-Aid for Scientific Research (B)

研究機関：Kagoshima University

2013年4月1日～ 2017年3月31日

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マダニの吸血および病原体感染防御において重要な役割を担うと考えられるToll 情報伝達経路を構成するToll 様関連因子(TLR)とTRAF関連因子(TRAFD1)の同定・特性について解明した。TRAFD1遺伝子をノックダウンすると、成ダニの吸血および産卵数に影響はないが、マダニの自然免疫関連分子であるディフェンシン様分子の発現が抑制された。一方、TLRはマダニによって媒介するバベシア原虫の増殖を抑制する機構に関与することが分かった。以上の結果より、TLRとTRAFD1はマダニの自然免疫系を制御する重要な分子である可能性が考えられた。
人工ヌクレアーゼTALENを用いた遺伝子ノックアウトマダニ感染症モデルの作出

田仲哲也, 望月雅美

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

研究種目：Grant-in-Aid for Challenging Exploratory Research

研究機関：Kagoshima University

2014年4月1日～ 2016年3月31日

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マダニの遺伝子機能の解析ではRNA干渉法による遺伝子破壊が用いられてきたが、ノックアウトやトランスジェニックの技術は導入されていない。そこで、我々はこれらの技術を確立する基盤として遺伝子導入マダニ細胞の作製を行った。プラスミドのマダニ細胞のトランスフェクション効率では、pmiGLO並びにpCAGGS-Lucefearaseが高いルシフェラーゼ活性を示したが、フタトゲチマダニ由来である、アクチンプロモーター並びにフェリチンプロモーターのマダニ細胞でのルシフェラーゼ活性は低かった。将来的にはこれらの研究手法によって、遺伝子ノックアウトやトランスジェニックマダニ作製の突破口となる。
マダニの生物活性分子(TBM)を用いた難治性原虫病の予防・治療開発

田仲哲也

2012年～ 2013年

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トキソプラズマ原虫やクリプトスポリジウム原虫はヒトや動物に寄生し、深刻な疾病を引き起こす難治性の原虫病である。我々は難治性原虫病に有効な抗原虫分子を探索していたところ、マダニの自然免疫を担うであるディフェンシン様分子のペプチド部位に抗トキソプラズマ効果を見出した。しかし、このペプチドに対してクリプトスポリジウム原虫に類似したアイメリア原虫のオーシストについては予防効果が認められなかった。今後は当初の目標を達成するために、他のペプチド部位の検索も行い、クリプトスポリジウム原虫とマラリア原虫に対する防御効果についてin vitro、in vivoレベルで解析を行う予定である。
牛乳中の高機能タンパク質の組換えウイルス用いた新規のマダニ防圧法開発

田仲哲也, 藤崎幸蔵, 玄学南

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

研究種目：Grant-in-Aid for Scientific Research (C)

研究機関：Kagoshima University

2010年～ 2012年

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ミルクタンパク質(high-potential milk protein: HMP)がマダニの発育・増殖に及ぼす可能性があると考えるに至り、マダニ吸血後のマダニ体内に含まれる HMP のラクトフェリンの検出を行ったが、宿主血液由来のラクトフェリンの存在が確認できなかった。そこで、ラクトフェリンと同じ性質を持つ宿主血液由来のトランスフェリンの検出を試みたところ、トランスフェリンは中腸から卵巣に到達することが確認された。これらの結果より、トランスフェリンは卵巣内の卵母細胞に鉄を供給する役割を果たしている可能性が示唆された。
マダニ媒介原虫病のTRANSMISSIONーBLOCKING VACCINE開発

藤崎幸藏, 田仲哲也, 鈴木宏志, 辻尚利

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

研究種目：Grant-in-Aid for Scientific Research (A)

研究機関：Kagoshima University

2007年～ 2010年

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(1)マダニによって人獣に媒介されるBabesia原虫のマダニ体内における生殖期・スポロゴニー期の虫体(ガメートからスポロゾイトに至る発育期の虫体)と顕著に反応・結合するマダニ生物活性物質(TBM)として、マダニの卵黄蛋白前駆体(Vg)とその受容体のVgR、ならびに血液消化酵素(システインプロテアーゼ)のlongipain を発見した。(2)VgとVgRは、RNA干渉法(RNAi)によって原虫媒介を完全に阻止し、抗体のワクチン効果も多大であったことから、現在、これらと結合する原虫分子の同定などの、Babesia媒介阻止ワクチンとしての応用に必要な研究を鋭意実施中である。(3)しかし、longipainは、RNAiによる遺伝子ノックダウンによって、B.gibsoni原虫の増殖を約3倍程度に増大させるものであり、遺伝子のover-expression(過剰発現)系が未開発の現状では、媒介阻止ワクチンして応用することは難しい現状にある。(4)一方、Babesiaに対する顕著な発育・増殖の阻止効果を有するTBMの探索を行う中で、細胞内寄生性のBabesiaの必須栄養源であるマダニ細胞内のバイオマスの動態に関わるTBMに興味を持ち、(5)マダニの細胞内蛋白の転写、翻訳開始、翻訳後修飾、小胞体・ゴルジ間輸送に関与する一連のTBMに加えて、細胞内蛋白質のバルク分解機構であるオートファジー(AT)に関連するTBM多数を同定・特性解明して、いずれも世界に先駆けて報告した。(6)これらの研究を通じて、アミノ酸シグナルによって細胞内タンパク質の動態を中枢的に制御しているTORカスケード(TORp)が、マダニの生存とマダニ細胞内の寄生原虫の増殖に果たす重要性の大きさが明らかになり、(7)TORp構成分子を媒介阻止ワクチンの候補分子として活用しうる可能性が示されてきた。
マダニの生物活性分子(TBM)の特性とその機能解明競争的資金

2007年～
遺伝子KOトキソプラズマ原虫作製法の確立と弱毒生ワクチン開発への応用

玄学南, 横山直明, 井上昇, 田仲哲也, 岩田晃, 高島康弘

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

研究種目：Grant-in-Aid for Scientific Research (B)

研究機関：Obihiro University of Agriculture and Veterinary Medicine

2002年～ 2005年

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1)トキソプラズマ原虫のベクター化トキソプラズマ原虫由来のピリメタミン耐性ジヒドロ葉酸レダクターゼチミジル酸シンターゼ(DHFR-TS)遺伝子と海洋生物由来の緑色蛍光タンパク質(GFP)遺伝子発現ユニットの間にマルチクローニングサイト(MCS)を持つトランスファーベクターpDMGを構築した。MCSに外来抗原遺伝子を挿入したpDMGプラスミドをElectroporation法によりトキソプラズマ原虫に導入した後に、ピリメタミンによる薬剤選択とGFPによるカラー選択を行ったところ、目的の外来抗原遺伝子を発現する組換えトキソプラズマ原虫を効率よく作製することに成功した。 2)ネオスポラ原虫のワクチン抗原NcSRS2を発現する組換えトキソプラズマ原虫の作製とワクチン効果の評価ネオスポラ原虫の主要ワクチン候補抗原NcSRS2遺伝子を発現する組換えトキソプラズマ原虫Tg/NcSRS2を作製した。1組換えTg/NcSRS2により発現されたNcSRS2の局在、分子量及び抗原性などはネオスポラ原虫由来の天然型NcSRS2に類似していることが示された。一組換えTg/NcSRS2で免疫したマウスではネオスポラ原虫に対する感染防御効果が認められた。 3)クリプトスポリジウム原虫のワクチン抗原CpP23を発現する組換えトキソプラズマ原虫の作製とワクチン効果の評価クリプトスポリジウム原虫の主要ワクチン候補抗原CpP23遺伝子を発現する組換えトキソプラズマ原虫Tg/CpP23を作製した。組換えTg/CpP23により発現されたCpP23の局在、分子量及び抗原性などはクリプトスポリジウム原虫由来の天然型CpP23に類似していることが示された。組換えTg/CpP23で免疫したマウスではクリプトスポリジウム原虫中和抗体の上昇が認められた。
原虫特異的アプタマーの作製および診断・治療への応用に関する基礎的研究

井上昇, 杉本千尋, 鈴木宏志, 田仲哲也

2002年～ 2003年

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1.リパノソーマ増殖に必須であることが報告されているLDLおよびトランスフェリン(TF)に着目し,これらの血漿成分に対するアプタマーが虫体の増殖を阻害するか否かを検討した。予備的に作製した抗牛LDLおよび抗牛TF抗体を用いてIn Vitroでの増殖阻害試験を行った結果,両抗体共に明らかな増殖阻止効果は認められなかった。 2.アプタマー作製用オリゴヌクレオチドを用いた抗トリパノソーマDNAアプタマーの作製においても現時点では期待する増殖阻止効果を示すアプタマーは得られていない。今後さらにペプチドアプタマーなども試験していく必要があると思われる。 3.トリパノソーマ抽出物およびトリパノソーマ虫体に対するDNA/RNAアプタマー作製を試みた。今後考慮・改善すべき点として、DNAまたはRNAアプタマーと細胞由来DNA/RNA結合性蛋白質などとの相互作用によってSELEX反応に予期せぬ選択圧が生じることが考えられる。細胞体あるいは細胞抽出物のような雑多な生体分子の混合物に対するアプタマー作製に当たってはペプチドアプタマーあるいはファージディスプレイなども利用すべきであろう。
トリパノソーマ病特異的診断法の開発および野外材料を用いた実用評価

井上昇, 玄学南, 田仲哲也, 横山直明, 長澤秀行, 藤崎幸蔵

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

研究種目：Grant-in-Aid for Scientific Research (B)

研究機関：Obihiro University of Agriculture and Veterinary Medicine

2001年～ 2003年

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LAMP法によるトリパノソーマ特異的遺伝子検出法の開発。 LAMP法は等温反応で高感度遺伝子増幅を行う方法である。また、遺伝子増幅の有無が反応液の濁度によって判別可能である。従って実施には単純なインキュベーター以外の特別な設備を要しない。LAMP法のこのような特徴は、十分な権設・設備が利用できない開発途上国や小規模診療施設などで高感度病原体検出を実施する上で非常に有用である。トリパノソーマ慢性感染マウスを用いた実験では、直接検出<PCR<LAMPの順に検出感度が高くなり、LAMP法の有用性が検証された。従来から指摘されていた通り本実験においても、LAMP法に用いるBst DNAポリメラーゼはPCR法に使用するTaq DNAポリメラーゼよりも血液由来酵素阻害因子の影響を受けにくいことを示唆する結果が得られた。タイ国・農務省・農産物食品局(National Bureau of Agricultural Commodity and Food Standards(ACFS), Ministry of Agriculture and Cooperatives, Bangkok, Thailand)のツンタスヴァン(D.Tuntasuvan)博士との共同研究で、トリパノソーマ感染ブタからのトリパノソーマ検出を、直接検出法(スメア法、遠心法)、マウス接種法、PCR法、LAMP法の各方法を用いて行った。その結果、LAMP法はPCR法と同等以上の高感度でトリパノソーマ遺伝子を検出した。反応所要時間および検出の簡便性ではLAMP法がPCR法よりも優れている。今回の研究で、LAMP法の野外使用における有用性は十分証明されたと考えるが、今後LAMP法に供するDNAサンプルの調製法については十分検討すべきである。LAMP法は高感度であるため、今回の研究においてもDNA調製段階での試料間の相互汚染が問題となることがあった。血清診断用抗原の探索トリパノソーマミトコンドリアHSP70およびリボソームP0蛋白質がアフリカトリパノソーマ検出ELISA抗原として有用であることが明らかとなった。リボソームP0蛋白質はC末端に強い抗原性を示す領域を有することも明らかとなった。P0-C末端領域の合成ペプチドを用いたELISAでもトリパノソーマ感染血清を良好に検出可能であった。合成ペプチドを使用することによって組換え蛋白質の発現と精製など大幅に診断系構築のステップを削減することが可能となる。
ウシラクトフェリンを用いたトキソプラズマ原虫の生体機能の解明

田仲哲也

2001年～ 2002年

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トランスフェリンファミリータンパク質であるラクトフェリン、トランスフェリン、オボトランスフェリンはそれぞれ哺乳類、鳥類などの生体内に分泌される分子量約80,000の鉄結合性タンパク質である。そこで本研究の目的は、トキソプラズマのラクトフェリン、トランスフェリン、オボトランスフェリン結合タンパク質を解析する事によって、原虫の鉄代謝機構を解明することこである。さらに本研究の成果は、抗生物質とは異なった新しい抗原虫病治療薬やワクチンの開発へつながるものと期待できる。そこで、我々はトキソプラズマ原虫のトランスファミリータンパク質に対する結合性タンパク質を解析するために、far Western blot法によって鉄結合性タンパク質とトキソプラズマ原虫の結合性について実験を行った。その結果、ウシラクトフェリン、ウシトランスフェリン、オボトランスフェリンは互いにトキソプラズマ中の同じ可溶性タンパク質に結合すること、その分子量は約42kDaであった(投稿準備中)。また興味深いことに、トランスフェリンレセプターを持つことが知られているトリパノソーマ原虫を用いて同様な実験を行ったところ、ウシラクトフェリン、ウシトランスフェリン、オボトランスフェリンは互いにトリパノソーマ中の同じ分子量約40、43kDa付近の2つのタンパク質と結合することが分った。これらの結果から、同じ原虫であっても、鉄結合性タンパク質に反応するタンパク質は原虫の種類によって異なることが分った。また、40kDaのラクトフェリン結合タンパク質についてN末端アミノ酸配列を決定したところ、グリセルアルデヒド-3-リン酸デヒドロゲナーゼであった。現在43kDaのラクトフェリン結合タンパク質についてはN末端アミノ酸配列を解析しているところである(投稿準備中)。今後はこれらのタンパク質の機能について検討するつもりである。
ウシラクトペルオキシダーゼとその組み換え体を用いた構造と機能の解析

島崎敬一, 田仲哲也, 玖村朗人, 中村信吾

提供機関：Japan Society for the Promotion of Science

制度名：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B).

研究種目：Grant-in-Aid for Scientific Research (B).

研究機関：HOKKAIDO UNIVERSITY

1999年～ 2000年

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牛乳に含まれる酸化還元酵素・ラクトペルオキシダーゼの示す抗菌・抗ウイルス活性を自由に操作出来るならば、医薬品や食品へ応用する際に非常な利点となると考えられる。そこで組換えラクトペルオキシダーゼを調製し、牛乳由来のラクトペルオキシダーゼと比較することで、活性に寄与する高次構造、およびヘム近傍の微細構造についての詳細な情報を得ることを目的としてこれまでに以下の実験を行った。〇ラクトペルオキシダーゼの検出に用いる目的で、ラクトペルオキシダーゼに対するポリクローナルおよびモノクローナル抗体を作成した。モノクローナル抗体については、ラクトペルオキシダーゼ分子内のエピトープ部位も決定した。〇ウシ乳腺細胞からラクトペルオキシダーゼ遺伝子をクローニングし、CHO細胞を用いて組換え体を発現させ、さらにイオン交換クロマトグラフィーにて精製した。発現した組換え体の確認は、抗ラクトペルオキシダーゼ抗体および活性測定にて行った。〇円偏光二色性スペクトルの測定により、牛乳ラクトペルオキシダーゼと高次構造が同じであることを確かめた。一方、牛乳ラクトペルオキシダーゼのN-末端アミノ酸部分は組換え体に比べて15〜20残基短かいことを見いだし、ミルク中に分泌される際の機構に新たな知見を加えた。〇牛乳から分離したラクトペルオキシダーゼおよび組換えラクトペルオキシダーゼをペプシン分解した後、ヘム結合フラグメントを逆相クロマトグラフィーにより分離することに成功した。現在、そのフラグメントのアミノ酸配列を決定中であり、ヘムとの結合様式を推定する手掛かりとなるものと期待される。

︎全件表示 ︎最初の5件までを表示