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Tetsuya Tanaka

Section

Graduate School of Agricultural Science

Job title

Professor

researchmap

https://researchmap.jp/read0065424

J-GLOBAL ID

200901067973854530

Profile

岐阜大学大学院連合獣医学研究科博士課程修了。博士（獣医学）。微生物化学研究所、帯広畜産大学原虫病分子免疫研究センター、北海道大学大学院農学研究科、ブリュッセル自由大学分子生物医科学研究所を経て、鹿児島大学農学部助教授、鹿児島大学共同獣医学部教授、2024年より現職。現在の専門分野は、節足動物を含めた病原体のタンパク質機能化学。主な論文に「マダニ、原虫、ウイルス、細菌、ミルクタンパク質」など169報。趣味は本屋巡り、食べ飲み歩き、山歩き。

Research History 9

2026 - Present

東北大学食と農免疫国際教育研究センター　教授（兼任）
2024 - Present

Tohoku University
2021 - Present

フィリピン大学ロスバニョス校　客員教授（兼任）
2002 - 2025

Obihiro University of Agriculture and Veterinary Medicine　National Research Center for Protozoan Diseases
2018 - 2024

Kagoshima University　Joint faculty of Veterinary Medicine
2013 - 2024

今村学園ライセンスアカデミー　講師
2013 - 2018

Kagoshima University　Joint faculty of Veterinary Medicine
2006 - 2012

Kagoshima University　Veterinary Medicine, Faculty of Agriculture
2006 - 2012

鹿児島大学フロンティアサンエンス研究推進センター准教授（兼任）

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Education 3

Gifu University　The United Graduate School of Veterinary Sciences

1993 - 1997
Obihiro University of Agriculture and Veterinary Medicine　Grduate School of Agriculture and Veterinary Medicine

1991 - 1993
Obihiro University of Agriculture and Veterinary Medicine　Faculty of Animal Husbandry

1987 - 1991

Committee Memberships 1

日本獣医学会、日本獣医寄生虫学会、日本寄生虫学会　評議員

Professional Memberships 4

日本獣医寄生虫学会
日本分子生物学会
日本寄生虫学会
日本獣医学会

Research Interests 3

感染症学
節足動物分子工学
タンパク質機能化学

Research Areas 3

Life sciences / Parasitology /
Environmental science/Agricultural science / Entomology /
Life sciences / Veterinary medicine /

Papers 187

Molecular survey and phylogenetic analysis of Borrelia theileri, Rickettsia aeschlimannii, Mycoplasma ovis, and Mycoplasma wenyonii in sheep and goats from southern Egypt. International-journal Peer-reviewed

Hassan Y A H Mahmoud, Ahmed M Soliman, Wafa Abdullah I Al-Megrin, Hind Alzaylaee, Tetsuya Tanaka, Walaa F A Emeish

Scientific reports　2025/12/29

DOI： 10.1038/s41598-025-32438-4 　
Molecular detection and phylogenetic characterization of Borrelia theileri and a putative novel Borrelia genotype in Hyalomma dromedarii ticks infesting camels in southern Egypt. International-journal Peer-reviewed

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Samah Mohamed, Tatsuro Hifumi, Kyoko Tsukiyama-Kohara, Tetsuya Tanaka

Comparative immunology, microbiology and infectious diseases　124　102403-102403　2025/09/05

DOI： 10.1016/j.cimid.2025.102403 　

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Ticks are important vectors of various pathogens affecting human and animal health globally. In Egypt, camels are frequently infested with Hyalomma dromedarii, yet their role in harboring and transmitting tick-borne pathogens remains poorly understood. This study aimed to molecularly detect and characterize Borrelia species in H. dromedarii ticks collected from camels in three cities in southern Egypt: Esna, Draw, and Edfu. Of 300 ticks analyzed using PCR targeting the 16S rRNA and flaB genes, 21.3 % (64/300) tested positive for Borrelia species. The highest prevalence was recorded in Edfu (37 %), followed by Draw (19 %) and Esna (13.3 %). Sequencing of the flaB gene revealed two distinct genetic groups. Phylogenetic analysis demonstrated that one group clustered closely with Borrelia theileri, while the second formed a distinct clade within the relapsing fever group of Borrelia species, showing sufficient genetic divergence to suggest a putative novel genotype or locally adapted strain. This clade was supported by high bootstrap values (99 %) and showed no close relation to previously described species. To our knowledge, this study represents the first detection of B. theileri in camel-associated ticks in Egypt. These findings expand the known genetic diversity of Borrelia species in North Africa and highlight the need for further studies to assess the pathogenic potential and epidemiological significance of these strains in animal and human populations.
First detection of Rickettsia japonica in Hyalomma dromedarii ticks infesting camels from Egypt: A call for enhanced surveillance of tick-borne pathogens. International-journal Peer-reviewed

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Samah Mohamed, Tatsuro Hifumi, Kyoko Tsukiyama-Kohara, Tetsuya Tanaka

Medical and veterinary entomology　40　(1)　163-169　2025/09/04

DOI： 10.1111/mve.70013 　

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Tick-borne rickettsiosis has posed a significant threat to Egypt, with various pathogenic Rickettsia species being reported. In this study, 134 ticks were collected from camels in Esna City, Luxor, Egypt and all were identified as Hyalomma dromedarii through both morphological and molecular techniques. Using specific primers targeting the citrate synthase (gltA), outer membrane protein A (ompA) and 17 kD antigen (17 kDa) genes, Rickettsia japonica was detected via conventional and nested PCR assays. Remarkably, two samples tested positive for R. japonica across all three genes, indicating a prevalence of 1.5%. Phylogenetic analysis based on the gltA, ompA and 17 kDa genes confirmed the presence of R. japonica within H. dromedarii ticks. This is the first reported detection of R. japonica in Egypt. These findings highlight the urgent need to investigate the origin and spread of R. japonica in the country and underscore the importance of continuous surveillance and data collection on lesser-known pathogens circulating in ticks as part of Egypt's public health efforts to manage tick-borne diseases.
First detection of Colpodella spp. in Rhipicephalus annulatus and molecular characterization of piroplasmids in southern Egypt. International-journal Peer-reviewed

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Samah Mohamed, Tatsuro Hifumi, Kyoko Tsukiyama-Kohara, Tetsuya Tanaka

Frontiers in veterinary science　12　1617204-1617204　2025/07/05

DOI： 10.3389/fvets.2025.1617204 　

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Piroplasmosis is a tick-borne disease that can affect livestock, particularly cattle. Its causative pathogens are intracellular apicomplexan parasites belonging to the order Piroplasmida. We recently identified one such emergent pathogen (Colpodella spp.) in ticks infesting camel in Egypt. Accordingly, we aimed to ascertain the presence of hemoprotozoan parasites in ticks infesting cattle. We removed ticks from household cattle during veterinary examinations, and submitted them for morphological examination and PCR analyses for species identification. Ticks and hemoprotozoan species obtained from tick samples were also evaluated using BLAST analysis, followed by confirmatory phylogenetic analyses. The collected ticks were identified as belonging to three species: Hyalomma dromedarii, Hyalomma marginatum, and Rhipicephalus annulatus. Phylogenetic analysis based on the 16S rRNA gene revealed that these ticks were clustered with those of the relevant species previously documented in Egypt. Molecular analysis targeting the 18S rRNA gene revealed Colpodella spp., the second such report in Egypt and the first in R. annulatus ticks infesting cattle. The Colpodella minimum infection rate (MIR) was 2.3% (per sample of pooled ticks from a single bovine host). Furthermore, Babesia bovis, Theileria. annulata, and Theileria orientalis were detected with MIRs of 3.5%, 4.7%, and 0.39%, respectively. In the phylogenetic analysis, each detected pathogen clustered with its corresponding species. Specifically, the Colpodella spp. were grouped with Colpodella spp. previously detected in Rhipicephalus microplus, Rhipicephalus haemaphysaloides ticks, and humans in China (accession numbers MH208620, MH208621, and GQ411073), and H. dromedarii ticks infesting camel in southern Egypt (accession numbers LC775361 and LC775361). We confirmed the detection of B. bovis and T. annulata through PCR assays with specific primers targeting the spherical body protein-4 gene and the major merozoite surface antigen gene, respectively. The detection of Colpodella spp. in ticks infesting cattle highlights the need for ongoing surveillance of this parasites. Both cattle and camels may serve as sentinel species, emphasizing the importance of monitoring these livestock for emerging parasites.
Vaccinomics-based identification of immunodominant epitopes in the tick-borne encephalitis virus polyprotein for multi-epitope vaccine development Peer-reviewed

Sudais Rahman, Da-Wei Huang, Mohibullah Shah, Mashal M. Almutairi, Iram Liaqat, Tetsuya Tanaka, Chien-Chin Chen, Sher Bahadar, Abid Ali

Computational and Structural Biotechnology Reports　2　100047-100047　2025/07/01
Publisher: Elsevier BV
DOI： 10.1016/j.csbr.2025.100047 　

ISSN： 2950-3639
Molecular prevalence and phylogenetic characterization of Francisella-like endosymbionts in ticks infesting camels and cattle from Southern Egypt. International-journal Peer-reviewed

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Samah Mohamed, Tatsuro Hifumi, Tetsuya Tanaka

Microbial pathogenesis　205　107639-107639　2025/04/25

DOI： 10.1016/j.micpath.2025.107639 　

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Ticks serve as vectors for numerous pathogens affecting human and animal health and harbor various microorganisms, including Francisella-like endosymbionts (FLEs), whose potential pathogenicity remains uncertain. In Egypt, data on FLEs, particularly in Southern Egypt, are limited, despite the importance of tick-borne pathogen surveillance in livestock. This study represents the first report of FLEs in Southern Egypt and the first detection of FLEs in Rhipicephalus annulatus in Egypt. A total of 726 ticks infesting cattle and camels were collected from various locations in Southern Egypt. Ticks were identified using both morphological and molecular techniques based on the 16S rRNA gene, and FLE detection was performed using PCR assays targeting a fragment of the Francisella 16S rRNA gene. Francisella DNA was detected in 60.1 % (288/479) of the examined ticks, with the highest prevalence in Hyalomma marginatum (100 %, 4/4), followed by Hyalomma dromedarii (74.8 %, 243/325), and R. annulatus (27.3 %, 41/150). Sequencing of 19 positive samples revealed that H. dromedarii (n = 13), H. marginatum (n = 2), and R. annulatus (n = 4) carried FLE sequences with 99-100 % similarity to strains previously identified in ticks worldwide. Given the high prevalence of FLEs in livestock-associated ticks, further research should focus on characterizing these strains, assessing their role in tick-borne pathogen ecology, and evaluating potential implications for human and animal health.
Molecular detection of Rickettsia aeschlimannii, Borrelia theileri, and Francisella-like endosymbionts in Camelus dromedarius and dogs in Luxor, Egypt. International-journal Peer-reviewed

Hassan Y A H Mahmoud, Ahmed M Soliman, Moshera S Shahat, Ali A Hroobi, Ali H Alghamdi, Abdullah M Almotayri, Tetsuya Tanaka, Walaa F A Emeish

Scientific reports　15　(1)　12872-12872　2025/04/15

DOI： 10.1038/s41598-025-91530-x 　

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Vector-borne bacterial pathogens can cause disease in a range of animals, including dromedary camels and dogs, but epidemiological and molecular studies on these pathogens are scarce in southern Egypt. In this study, we screened camels and dogs in southern Egypt (Luxor) for vector-borne bacterial pathogens, with molecular analysis of 200 blood samples collected from camels and dogs in the region. The Rickettsia aeschlimannii gltA gene was detected in 5% (5/100) of camel blood samples and 1% (1/100) of dog blood samples. This study is the first report of Rickettsia aeschlimannii in camel blood in southern Egypt. Additionally, the 16S rRNA gene of a Francisella-like endosymbiont was detected in both camel and dog blood for the first time, with infection rates of 2% (2/100) in camels and 2% (2/100) in dogs. In dog blood, the Borrelia theileri flaB gene was detected for the first time in southern Egypt at a positivity rate of 5% (5/100). Neither Coxiella nor Bartonella species were detected in this study. In southern Egypt, Rickettsia aeschlimannii, Borrelia theileri, and Francisella-like endosymbionts were detected in camels and dogs, providing valuable information about their infection rate and these findings contribute to a better understanding of their transmission dynamics.
First detection and molecular characterization of Mycoplasma species in camels, cattle, buffalo, and their associated ticks from southern Egypt. International-journal Peer-reviewed

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Samah Mohamed, Tatsuro Hifumi, Abdelrahman A Rady, Alsagher O Ali, Walaa F A Emeish, Tetsuya Tanaka

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases　105750-105750　2025/04/08

DOI： 10.1016/j.meegid.2025.105750 　

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Mycoplasma species are wall-less bacteria that infect multiple mammalian orders, including livestock, and are associated with economically significant diseases. In Egypt, molecular data on the presence and diversity of Mycoplasma spp. in camels, cattle, buffalo, and their associated ticks remain limited, despite their potential impact on livestock health and productivity. This study aimed to detect and genetically characterize Mycoplasma spp. in cattle, buffalo, camels, and their associated ticks in southern Egypt. A total of 571 samples (384 ticks, 50 buffalo, 68 cattle, and 69 camels) were screened using PCR targeting the 16S rRNA gene, followed by phylogenetic analysis of representative amplicons. Hyalomma dromedarii, Hyalomma marginatum, and Rhipicephalus annulatus were the tick species collected. Overall, Mycoplasma DNA was detected in 10.3 % of the samples. Mycoplasma ovis-like was identified in 2.9 % of camel blood, 9.4 % of H. dromedarii, 75 % of H. marginatum, and 13.8 % of R. annulatus ticks. Candidatus Mycoplasma haematovis was found in 0.4 % of H. dromedarii, and Mycoplasma wenyonii-like in 13.2 % of cattle and 4 % of buffalo blood. Remarkably, this is the first global detection of M. ovis in camel blood, as well as the detection of M. ovis-like and Ca. M. haematovis in ticks circulating on camels. This study also reports the first detection of M. wenyonii-like in bovines in Egypt and documents M. ovis-like in three tick species parasitizing cattle. The genetic similarities of Mycoplasma sequences from ticks and livestock suggest that these arthropods may serve as potential vectors. These findings highlight the need for additional studies on the zoonotic potential, infectivity, and vector competence of Mycoplasma spp. in Egypt.
Prediction of potential drug targets and key inhibitors (ZINC67974679, ZINC67982856, and ZINC05668040) against Rickettsia felis using integrated computational approaches. International-journal Peer-reviewed

Sudais Rahman, Hsien Liu, Mohibuallah Shah, Mashal M Almutairi, Iram Liaqat, Tetsuya Tanaka, Chien-Chin Chen, Abdulaziz Alouffi, Abid Ali

Frontiers in veterinary science　11　1507496-1507496　2025/01/16

DOI： 10.3389/fvets.2024.1507496 　

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Rickettsia felis, responsible for flea-borne spotted fever, is a rising zoonotic pathogen posing an increasing global threat due to its expanding geographical distribution. The rise in antibiotic-resistant strains of this pathogen underscores the urgent need for new therapeutic interventions. This study employed a comprehensive subtractive proteomics analysis of the R. felis proteome, aiming to identify essential, non-host homologous, and pathogen-specific proteins, which were subsequently evaluated as potential new drug targets. These findings offer valuable insights into the development of therapeutic strategies against rickettsiosis. The analysis revealed 343 proteins that are non-homologous to the host, including 108 essential proteins, 25 unique metabolic pathways, and 11 distinct proteins. Out of these, 10 proteins were druggable in which two associated with virulence, and one related to resistance (succinate dehydrogenase). Through a rigorous screening process and extensive literature review, succinate dehydrogenase emerged as a promising drug target. Protein interaction partners for succinate dehydrogenase were identified using the STRING database. To further assess the functionality of succinate dehydrogenase, structure-based studies were conducted. Approximately 18,000 ZINC compounds were screened, leading to the finding of six potential inhibitors: ZINC67847806, ZINC67982856, ZINC67974679, ZINC67895371, ZINC05668040, and ZINC05670149. Absorption, distribution, metabolism, excretion, and toxicity (ADMET) profiling confirmed that most compounds met the preferred pharmacokinetic properties, except for ZINC67895371 and ZINC67847806, which exhibited positive ames test results, and ZINC05670149, ZINC67895371, and ZINC67847806, showed hepatotoxicity. All compounds were found to be non-sensitizing to the skin. Based on these findings, further experimental validation of ZINC67974679, ZINC67982856, and ZINC05668040 is recommended.
Teratological abnormalities in hard ticks (Acari: Ixodidae) from Pakistan. International-journal Peer-reviewed

Ejaz Akbar, Shafi Ullah, Mashal M Almutairi, Iram Liaqat, Muhammad Adnan, Abdulaziz Alouffi, Tetsuya Tanaka, Abid Ali

Experimental & applied acarology　94　(1)　18-18　2024/12/26

DOI： 10.1007/s10493-024-00989-2 　

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Studying teratological abnormalities in ticks are taxonomically important because this poorly understood biological phenomenon causes difficulties in tick's identification. Globally, reports regarding these abnormalities in ticks, reasons of their causes and their impacts are scarce. According to the available published data, there are no studies regarding teratological abnormalities in ticks from Pakistan. Herein, ticks examined for these abnormalities were museum deposited, collected from buffalos, cattle, camels, goats and sheep in 17 districts of Pakistan during 2017-2023. The abnormalities were observed in 88 out of 3279 (2.68%) ticks, representing eight ixodid species (Rhipicephalus microplus, Rhipicephalus turanicus, Rhipicephalus haemaphysaloides, Haemaphysalis bispinosa, Haemaphysalis montgomeryi, Hyalomma anatolicum, Hyalomma scupense, Hyalomma dromedarii). Among abnormal ticks, 67 abnormalities were characterized as local, 12 as general and nine as a combination of both. Local abnormalities were observed in the scutum, palpal segments, anal aperture, spiracular plates, ventral plates, caudal appendage, festoons and posterior grooves. Also, the constrictions and excavations of body parts were observed. General abnormalities included irregular idiosoma and lateral idiosomal bifurcation. This study, for the first time, highlights the diversity of teratological abnormalities in Pakistani ticks, and are reported in in Hy. anatolicum, Ha. bispinosa and Ha. montgomeryi for the first time.
Investigation of Hyalomma turanicum and Hyalomma asiaticum in Pakistan, with notes on the detection of tickborne Rickettsiales. International-journal Peer-reviewed

Zafar Ullah, Iram Liaqat, Mehran Khan, Abdulaziz Alouffi, Mashal M Almutairi, Dmitry A Apanaskevich, Tetsuya Tanaka, Abid Ali

Frontiers in veterinary science　11　1500930-1500930　2024/12/23

DOI： 10.3389/fvets.2024.1500930 　

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There is limited information on the occurrence of Hyalomma turanicum and Hyalomma asiaticum ticks, as well as associated Rickettsia, Anaplasma, and Ehrlichia species in Pakistan. Addressing this knowledge gap, the current study aimed at morphomolecular confirmation of these ticks and molecular assessment of associated Rickettsiales bacteria (Rickettsia, Anaplasma, and Ehrlichia spp.) in Balochistan, Pakistan. A total of 314 ticks were collected from 74 of 117 (63.2%) hosts, including 41 of 74 (55.4%) goats and 33 of 74 (44.5%) sheep. Subsequently, a subset of microscopically identified ticks was subjected to DNA extraction and PCR to amplify 16S rDNA and cox1 fragments. Additionally, gltA, ompA, and ompB fragments were targeted for Rickettsia spp. and 16S rDNA fragments for both Anaplasma and Ehrlichia spp. The 16S rDNA and cox1 sequences of Hy. turanicum demonstrated 100% identity with those of the same species previously reported from Pakistan. The 16S rDNA and cox1 sequences of Hy. asiaticum exhibited 99.52 and 100% identities, respectively, with corresponding species reported from China, Kazakhstan, and Turkey. The gltA, ompA, and ompB fragments associated with Hy. turanicum showed 100% identities with Rickettsia aeschlimannii reported from Egypt, Italy, Kazakhstan, Kenya, Pakistan, and Senegal. The 16S rDNA sequences of Anaplasma sp. and Ehrlichia sp. associated with both Hy. asiaticum and Hy. turanicum exhibited 99.67 and 100% identities with unknown Anaplasma sp. and Ehrlichia sp. reported from Morocco and Pakistan, respectively. In the 16S rDNA and cox1 phylogenetic trees of ticks, Hy. turanicum and Hy. asiaticum from the current study clustered with their respective species. Similarly, in gltA, ompA, and ompB phylogenetic trees of Rickettsia, R. aeschlimannii of the present study clustered with the same species, whereas Anaplasma sp. and Ehrlichia sp. of this study clustered with undetermined Anaplasma spp. and Ehrlichia spp. in the 16S rDNA phylogenetic tree of Anaplasmataceae. Among the DNA samples from the screened ticks, a coinfection rate of R. aeschlimannii, Anaplasma sp., and Ehrlichia sp. (2 of 80, 2.5%) was observed in Hy. turanicum, whereas individual infection rates were noted as follows: R. aeschlimannii (8 of 80, 10%), Anaplasma sp. (5 of 80, 6.3%), and Ehrlichia sp. (5 of 80, 6.3%). This study marks the first record of molecular characterization of Hy. turanicum and Hy. asiaticum as well as the detection of associated R. aeschlimannii, Anaplasma sp., and Ehrlichia sp. in Balochistan, Pakistan.
Unveiling Misconceptions among Small-Scale Farmers Regarding Ticks and Tick-Borne Diseases in Balochistan, Pakistan. International-journal Peer-reviewed

Zafar Ullah, Mehran Khan, Iram Liaqat, Kashif Kamran, Abdulaziz Alouffi, Mashal M Almutairi, Tetsuya Tanaka, Abid Ali

Veterinary sciences　11　(10)　2024/10/12

DOI： 10.3390/vetsci11100497 　

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Ticks and tick-borne diseases (TBDs) pose potential health threats to small-scale farmers of grazing animals in the upper highlands of Balochistan, Pakistan. This study was conducted based on a questionnaire survey involving 153 farmers of grazing animals in seven districts to access their knowledge, attitudes, and practices regarding ticks and TBDs. Odds ratios and 95% confidence intervals, based on Fisher's test, were used to assess risk factors for determining preventive measures. The findings revealed a low level of knowledge among the participants. For instance, there was a lack of awareness of the effects of climate change and the economic impact of ticks on animal health. The essential precautions, such as the non-indiscriminate use of acaricides, wearing dark-colored clothing, and limiting children's interaction with grazing animals, were often overlooked. However, the farmers had a positive attitude towards tick control, but they mostly relied on the knowledge of local communities. The neglect of such measures places these farmers and their children at risk of contracting TBDs. This study also indicates minimal involvement from the government in educating farmers and controlling ticks. The role of stakeholders, including the government, non-governmental organizations, veterinary doctors, and local farmer communities, is crucial to address these issues and to implement effective training programs that address misconceptions about ticks and TBDs. Overall, this study highlights the importance of implementing awareness and education programs to address the misconceptions about ticks and TBDs among farmers.
Molecular detection of Rickettsia aeschlimannii, Candidatus Rickettsia shennongii, Rickettsia sp. and Coxiella burnetii in ticks collected from camels. International-journal Peer-reviewed

Shafi Ullah, Abdulaziz Alouffi, Majid Khan, Momin Khan, Hadia Tila, Tetsuya Tanaka, Itabajara da Silva Vaz Jr, Mashal M Almutairi, Abid Ali

Scientific reports　14　(1)　22129-22129　2024/09/27

DOI： 10.1038/s41598-024-73663-7 　

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Tick-borne bacteria of the genera Rickettsia and Coxiella cause several emerging veterinary and human infectious diseases. Ticks of the genus Hyalomma are medically important vectors due to their potential role in the transmission of pathogens to vertebrate hosts. There is an inadequate knowledge on tick-borne Rickettsia spp. and Coxiella spp. in ticks infesting transhumant camels in Pakistan. In this study, we conducted a molecular survey for screening of Rickettsia spp. and Coxiella spp. in ticks infesting camels. Seven hard tick species including Hyalomma dromedarii, Hyalomma anatolicum, Hyalomma scupense, Hyalomma isaaci, Hyalomma turanicum, Hyalomma asiaticum, and Rhipicephalus sanguineus s.l were confirmed on camels in three distinct physiographic regions of Khyber Pakhtunkhwa, Pakistan. A subset of morphologically identified ticks were subjected to molecular assays for the genetic characterization of ticks and the detection and genetic characterization of Rickettsia and Coxiella species using standard genetic markers. Ticks screened for pathogens resulted in the detection of Rickettsia aeschlimannii and Candidatus Rickettsia shennongii and Coxiella burnetii. The molecular analysis further reveals the presences of an undetermined Rickettsia aeschlimannii-like species, that is making a distinct phylogenetic clade with R. aeschlimannii. The detection of pathogens in camel ticks poses potential health hazards as these ticks frequently bites humans. Molecular screening of Rickettsia spp. and Coxiella spp. associated with camel ticks is a preliminary step toward the surveillance of evaluating their zoonotic threats in the region.
Babesia bigemina and Theileria annulata infections in cattle: molecular detection, phylogenetic analysis, and assessment of risk factors. International-journal Peer-reviewed

Majid Khan, Mehran Khan, Momin Khan, Sidra Batool, Tetsuya Tanaka, Abdulaziz Aloufi, Mashal M Almutairi, Abid Ali

Tropical animal health and production　56　(8)　282-282　2024/09/26

DOI： 10.1007/s11250-024-04122-8 　

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Babesia bigemina and Theileria annulata are tick-borne protozoans that cause piroplasmosis in cattle, resulting in huge damages to the livestock industry. The prevalence of these infections depends on various intrinsic and extrinsic risk factors. In Pakistan, there is no information regarding the molecular characterization of Babesia bigemina and the risk factors associated with piroplasmosis. This study aimed to molecularly characterize Babesia spp. and Theileria spp. infecting various cattle breeds in Khyber Pakhtunkhwa, Pakistan, and to shed light on risk factors associated with these infections. Altogether, 219 blood samples were collected from various symptomatic cattle breeds, including Holstein Friesian (65.3%; 143/219), Jersey (21.5%; 47/219) and Sahiwal (13.2%; 29/219). Isolated genomic DNA from these blood samples was used in PCR for the amplification of the 18S rRNA fragment of apicomplexan parasites. Obtained 18S rDNA sequences from cattle hosts showed 99.5% identity with B. bigemina, or 100% with T. annulata. Having an overall infection rate of 61.6% (135/219), the highest infection rate was recorded for T. annulata (43.8%; 95/219), followed by B. bigemina (18.3%; 40/219). Phylogenetic analysis of 18S rDNA sequences revealed that B. bigemina clustered with corresponding species reported from Bolivia, and South Africa, while T. annulata grouped with same species from Italy, India, and Turkey. Among the different risk factors, the breed, season, and tick infestation were found to have a significant (P < 0.05) association with the piroplasmid infections. The information obtained in this study can be employed for effective surveillance and control of babesiosis and theileriosis in Pakistan. In addition to confirming our previous molecular detection of T. annulata in cattle, this study provides the first molecular surveillance and phylogenetic position of B. bigemina and associated risk factors in the study region.
Molecular detection of Anaplasma bovis, Candidatus Anaplasma boleense and Rickettsia spp. in ticks infesting small ruminants. International-journal Peer-reviewed

Zaibullah Khan, Farman Ullah, Shafi Ullah, Mohammed Ibrahim, Momin Khan, Gauhar Rehman, Tetsuya Tanaka, Mashal M Almutairi, Abdulaziz Alouffi, Abid Ali

BMC veterinary research　20　(1)　408-408　2024/09/11

DOI： 10.1186/s12917-024-04259-6 　

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Anaplasma spp. and Rickettsia spp. are intracellular vector-borne pathogens and harbored by a wide range of ticks and vertebrate hosts. Aim of this study was to molecularly characterize Anaplasma spp. and Rickettsia spp. in different ticks collected from livestock hosts in nine districts of Khyber Pakhtunkhwa (KP), Pakistan. In total, 862 ticks were collected from cattle, goats and sheep. Highest tick's infestation was observed on cattle 56.14% (32/57), followed by goats 45.45% (40/88), and sheep 42.05% (45/107). Rhipicephalus microplus (305/862, 35.38%) was predominant species, followed by Haemaphysalis sulcata (243/862, 28.19%), Hyalomma anatolicum (133/862, 15.42%), Haemaphysalis bispinosa (120/862, 13.92%), and Hyalomma kumari (61/862, 7.07%). A subset of 135 ticks were screened for Anaplasma spp. and Rickettsia spp. based on the amplification of partial 16 S rDNA and outer-membrane protein A (ompA) fragments, respectively. In total, 16 ticks (11.85%) were positive for Anaplasma spp. and Rickettsia spp. Obtained 16 S rDNA sequences for Anaplasma spp. detected in Ha. bispinosa and Ha. sulcata showed 99.98% identity with Anaplasma bovis, while other detected in Rh. microplus showed 99.84% identity with Candidatus Anaplasma boleense. Similarly, detected ompA sequence in Ha. sulcata showed 100% identity with Rickettsia sp. and 97.93% with Rickettsia slovaca, and another sequence detected in Rh. microplus showed 100% identity with Candidatus Rickettsia shennongii. In phylogenetic trees, these sequences clustered with corresponding species from Pakistan, China, Turkey, South Korea, South Africa, and Herzegovina. This is the first study reporting detection of A. bovis in Ha. bispinosa and Ha. sulcata, Ca. A. boleense in Rh. microplus collected from goats, and R. slovaca-like in Ha. sulcata. Our results enforce the need for regular surveillance of Rickettsiales in hard ticks infesting livestock in the region.
Targeting Yezo Virus Structural Proteins for Multi-Epitope Vaccine Design Using Immunoinformatics Approach. International-journal Peer-reviewed

Sudais Rahman, Chien-Chun Chiou, Mashal M Almutairi, Amar Ajmal, Sidra Batool, Bushra Javed, Tetsuya Tanaka, Chien-Chin Chen, Abdulaziz Alouffi, Abid Ali

Viruses　16　(9)　2024/09/03

DOI： 10.3390/v16091408 　

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A novel tick-borne orthonairovirus called the Yezo virus (YEZV), primarily transmitted by the Ixodes persulcatus tick, has been recently discovered and poses significant threats to human health. The YEZV is considered endemic in Japan and China. Clinical symptoms associated with this virus include thrombocytopenia, fatigue, headache, leukopenia, fever, depression, and neurological complications ranging from mild febrile illness to severe outcomes like meningitis and encephalitis. At present, there is no treatment or vaccine readily accessible for this pathogenic virus. Therefore, this research employed an immunoinformatics approach to pinpoint potential vaccine targets within the YEZV through an extensive examination of its structural proteins. Three structural proteins were chosen using specific criteria to pinpoint T-cell and B-cell epitopes, which were subsequently validated through interferon-gamma induction. Six overlapping epitopes for cytotoxic T-lymphocytes (CTL), helper T-lymphocytes (HTL), and linear B-lymphocytes (LBL) were selected to construct a multi-epitope vaccine, achieving a 92.29% coverage of the global population. These epitopes were then fused with the 50S ribosomal protein L7/L12 adjuvant to improve protection against international strains. The three-dimensional structure of the designed vaccine construct underwent an extensive evaluation through structural analysis. Following molecular docking studies, the YEZV vaccine construct emerged as a candidate for further investigation, showing the lowest binding energy (-78.7 kcal/mol) along with favorable physiochemical and immunological properties. Immune simulation and molecular dynamics studies demonstrated its stability and potential to induce a strong immune response within the host cells. This comprehensive analysis indicates that the designed vaccine construct could offer protection against the YEZV. It is crucial to conduct additional in vitro and in vivo experiments to verify its safety and effectiveness.
Molecular detection and characterization of Anaplasma marginale and Babesia canis vogeli infecting dogs in Luxor, Egypt. International-journal Peer-reviewed

Hassan Y A H Mahmoud, Moshera S Shahat, Ragab M Fereig, Alsagher O Ali, Walaa F A Emeish, Ahmed M Soliman, Fatma A Khalifa, Tetsuya Tanaka

Scientific reports　14　(1)　16888-16888　2024/07/23

DOI： 10.1038/s41598-024-67009-6 　

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Tick-borne diseases in animals are increasing rapidly worldwide, but there is insufficient information about tick-borne diseases infecting dogs in southern Egypt. Thus, in the current study, we detected the presence of Anaplasma marginale (A. marginale) and Babesia canis vogeli (B. canis vogeli) in the blood of dogs. The results revealed that 4/100 (4%) were positive, and a higher infection rate was found in males (75%), than females (25%). The phylogenetic analysis for the major surface protein 4 (msp4) gene in this study was compared with amplicons separate from other reported isolates with alignment by identity 100% with cattle and camels from Egypt, and the phylogenetic analysis for the B. canis vogeli small subunit ribosomal RNA (SSU rRNA) gene in this study identified identity by 99.89% with dogs from Egypt. This report is considered the first report in southern Egypt about A. marginale in dogs based on the sequence analysis of the msp4 gene, providing new data for the classification and identification of A. marginale in dogs compared to A. marginale isolated from other animals in southern Egypt.
Molecular detection and characterization of Anaplasma ovis, Theileria ovis, and Theileria lestoquardi in sheep and goats in Luxor, Egypt. International-journal Peer-reviewed

Hassan Y A H Mahmoud, Tetsuya Tanaka, Alsagher O Ali, Walaa F A Emeish

BMC veterinary research　20　(1)　260-260　2024/06/17

DOI： 10.1186/s12917-024-04109-5 　

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BACKGROUND: Tick-borne diseases cause economically significant losses to animal production globally, and anaplasmosis and theileriosis are associated with the greatest losses. However, the spread of the relevant pathogens in flocks of domesticated animals in southern Egypt is little understood. Accordingly, in this study, we aimed to determine the prevalences of Anaplasma ovis, Theileria ovis, and Theileria lestoquardi in southern Egyptian sheep and goats through blood tests, and to make a molecular characterization of the A. ovis detected in sheep targeting a specific gene. RESULTS: We collected blood samples collected from 300 sheep and goats (n=150 /species) in Luxor Province in southern Egypt, and analyzed them for the presence of A. ovis, T. ovis and T. lestoquardi with screening by conventional and nested PCR targeting the msp4 and msp5, 18S rRNA, and merozoite surface protein genes. For A. ovis 140/300 samples (46.66%) were positive overall, with 90/150 (60%) and 50/150 (33.33%) positive samples in sheep and goats, respectively. Two major surface protein genes of A. ovis, msp4 and msp5, were sequenced using DNA extracted from sheep and goat blood samples, for phylogenetic analysis and genotyping. The msp4 gene sequence revealed no significant genetic diversity, to contrast to data on A. ovis strains from other countries. For T. lestoquardi, 8/150 (5.33%) samples were positive in sheep, but no samples were positive in goats (0%). For T. ovis, 32/150 (21.33%) samples were positive in sheep, but no samples were positive in goats (0%). Sequencing targeting the merozoite surface protein gene for T. lestoquardi and the small subunit ribosomal RNA gene for T. ovis revealed no significant genetic diversity in the study, another contrast to data on A. ovis strains from other countries. CONCLUSION: This study provides valuable data on phylogenetic and molecular classifications of A. ovis, T. ovis and T. lestoquardi found in southern Egyptian sheep and goats. It also represents the first report on detection and molecular characterization of T. lestoquardi in southern Egyptian sheep based on the specific merozoite surface protein gene, thus providing valuable data for molecular characterization of this pathogen in southern Egypt.
Subtractive Proteomics and Reverse-Vaccinology Approaches for Novel Drug Target Identification and Chimeric Vaccine Development against Bartonella henselae Strain Houston-1. International-journal Peer-reviewed

Sudais Rahman, Chien-Chun Chiou, Shabir Ahmad, Zia Ul Islam, Tetsuya Tanaka, Abdulaziz Alouffi, Chien-Chin Chen, Mashal M Almutairi, Abid Ali

Bioengineering (Basel, Switzerland)　11　(5)　2024/05/17

DOI： 10.3390/bioengineering11050505 　

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Bartonella henselae is a Gram-negative bacterium causing a variety of clinical symptoms, ranging from cat-scratch disease to severe systemic infections, and it is primarily transmitted by infected fleas. Its status as an emerging zoonotic pathogen and its capacity to persist within host erythrocytes and endothelial cells emphasize its clinical significance. Despite progress in understanding its pathogenesis, limited knowledge exists about the virulence factors and regulatory mechanisms specific to the B. henselae strain Houston-1. Exploring these aspects is crucial for targeted therapeutic strategies against this versatile pathogen. Using reverse-vaccinology-based subtractive proteomics, this research aimed to identify the most antigenic proteins for formulating a multi-epitope vaccine against the B. henselae strain Houston-1. One crucial virulent and antigenic protein, the PAS domain-containing sensor histidine kinase protein, was identified. Subsequently, the identification of B-cell and T-cell epitopes for the specified protein was carried out and the evaluated epitopes were checked for their antigenicity, allergenicity, solubility, MHC binding capability, and toxicity. The filtered epitopes were merged using linkers and an adjuvant to create a multi-epitope vaccine construct. The structure was then refined, with 92.3% of amino acids falling within the allowed regions. Docking of the human receptor (TLR4) with the vaccine construct was performed and demonstrated a binding energy of -1047.2 Kcal/mol with more interactions. Molecular dynamic simulations confirmed the stability of this docked complex, emphasizing the conformation and interactions between the molecules. Further experimental validation is necessary to evaluate its effectiveness against B. henselae.
Discovery of Colpodella spp. in ticks (Hyalomma dromedarii) infesting camels in southern Egypt. International-journal Peer-reviewed

Ahmed M Soliman, Hassan Y A H Mahmoud, Tatsuro Hifumi, Tetsuya Tanaka

Ticks and tick-borne diseases　15　(5)　102352-102352　2024/05/16

DOI： 10.1016/j.ttbdis.2024.102352 　

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In Egypt, tick-borne diseases pose a significant threat to human and animal health, and the threat to dromedaries (Camelus dromedarius), the country's dominant camelid species, is of particular concern. These animals are frequently infested with ticks, and may thus develop tick-borne diseases or become reservoirs of tick-borne pathogens. However, there is a paucity of data on tick infestation in Egyptian camels, especially in the south of the country. Accordingly, we aimed to determine the prevalence of tick infestation in southern Egyptian camel populations (in Luxor and Aswan governorates), and identify the hemoprotozoan parasites carried by camel-infesting ticks. Camels were checked for ticks during veterinary examination at quarantine and household checks, and ticks were collected from infested camels for species identification using morphological examination and PCR analyses. Tick and hemoprotozoan species were identified using Basic Local Alignment Search Tool analysis with subsequent confirmation in phylogenetic analyses. All camel-infesting ticks belonged to the species Hyalomma dromedarii, and were clustered with ticks of this species previously found in Egypt in a phylogenetic tree based on the 16S rRNA gene. Molecular analysis targeting the 18S rRNA gene revealed the presence of hitherto undetected hemoprotozoan parasites, Colpodella spp., in 30/297 (10.1 %) camel-infesting ticks. In phylogenetic analysis, these Colpodella spp. were highly homologous (94-98.6 %) with Colpodella spp. previously deposited in GenBank with accession numbers OQ540590Q, MH208621, and GQ411073, which relate to Colpodella spp. previously detected from Haemaphysalis longicornis, Rhipicephalus haemaphysaloides, and humans in China. PCR analyses with spherical body protein-4 (SBP-4) gene-specific primers revealed Babesia bovis in 16/297 (5 %) of camel-infesting ticks, however, Babesia bigemina and Theileria annulata were not detected. Here, we report the first detection of Colpodella spp. in H. dromedarii in Egypt. Further epidemiological studies are needed to assess the risk to camels and humans, and the transmission dynamics. Based on the high tick infestation rates in Egyptian camels and the identification of previously unreported protozoan hemoparasites in ticks, we consider that the dromedary should be subject to surveillance as a sentinel species for tick-borne diseases in Egypt. Our findings underline the need for surveillance and collecting data on lesser known pathogens circulating in camel-infesting ticks, as part of a public health strategy for dealing with tick-borne diseases in Egypt.
Molecular detection and characterization of Theileria annulata, Babesia bovis, and Babesia bigemina infecting cattle and buffalo in southern Egypt. International-journal Peer-reviewed

Hassan Y A H Mahmoud, Abdelrahman A Rady, Tetsuya Tanaka

Parasite epidemiology and control　25　e00340　2024/05

DOI： 10.1016/j.parepi.2024.e00340 　

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Tick-borne diseases have a major adverse effect on livestock worldwide, causing enormous economic losses in meat and milk production as well threatening animal and public health. In this study, we aimed to detect and characterize piroplasms isolated from cattle and buffalo in southern Egypt, using molecular techniques. Three hundred blood samples were collected from cattle and buffalo in two governorates in southern Egypt. All 300 samples (100%) were confirmed to contain DNA, as they exhibited bands of bovine β-actin gene at the expected 227 bp for cattle and buffalo. The samples were analyzed by PCR for the presence of piroplasms, specifically Babesia bovis, Babesia bigemina, and Theileria annulata. Samples positive for the piroplasma 18S ribosomal RNA gene were further examined for two additional genes, spherical body protein 4 gene, to provide an enhanced degree of specificity for the identification of B. bovis and B. bigemina, and the major merozoite surface antigen gene for T. annulata. The infection rate for piroplasma spp. was 60/300 (20%). The positivity rates were 10.7% (32/300) for T. annulata, 5.3% (16/300) for B. bovis, and 4% (12/300) for B. bigemina. By host species, 42/150 (28%) cattle and 18/150 (12%) buffalo were positive for piroplasms. None of the isolates sequenced for the B. bovis isolates from buffalo in this study showed 100% identity with any sequence deposited in GenBank for the small subunit ribosomal RNA gene (maximum identity value = 99.74%). Similarly, no T. annulata small subunit ribosomal RNA gene sequence identified in this study exhibited 100% identity with any sequence deposited in GenBank (maximum identity value = 99.89%). The current study provides a partial sequence of the T. annulata merozoite-piroplasm surface antigen gene, as well as the B. bovis and B. bigemina spherical body protein 4 genes, in cattle and buffalo in southern Egypt, and is the first report on these piroplasma genes in cattle and buffalo in southern Egypt.
Molecular detection and diversity of tick-borne rickettsial pathogens in ticks collected from camel (Camelus dromedarius) in Upper Egypt. International-journal Peer-reviewed

Ahmed M Soliman, Hassan Y A H Mahmoud, Moaz M Amer, Tatsuro Hifumi, Tetsuya Tanaka

Acta tropica　253　107172-107172　2024/03/05

DOI： 10.1016/j.actatropica.2024.107172 　

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Tick-borne rickettsial pathogens pose significant threats to public and animal health. In Upper Egypt, limited information exists regarding the prevalence and diversity of such tick-borne pathogens. Therefore, this study aimed to conduct a comprehensive investigation to elucidate the presence and variety of tick-borne rickettsial pathogens in Upper Egyptian camels. Our results revealed a prevalence of 2.96 % for Anaplasma marginale and 0.34 % for Candidatus Anaplasma camelii among Hyalomma ticks. However, Ehrlichia spp. weren't detected in our study. The identification of Ca. A. camelii in H. dromedari ticks was documented for the first time, suggesting a potential mode of transmission in camels. Notably, this study marks the first documentation of Rickettsia aeschlimannii with a prevalence of 6.06 % in the study area. Furthermore, we detected Coxiella burnetii in a prevalence of 8.08 % in Hyalomma ticks, indicating a potential risk of Q fever transmission. Molecular techniques results were confirmed by sequencing and phylogenetic analysis and provided valuable insights into the epidemiology of these pathogens, revealing their diversity. This study is vital in understanding tick-borne rickettsial pathogens' prevalence, distribution, and transmission dynamics in Upper Egypt. In conclusion, our findings emphasize the importance of continued research to enhance our understanding of the epidemiology and impact of these pathogens on both animal and human populations.
Molecular phylogenetic analysis of Echinococcus multilocularis from horses raised in Canada or Japan, using mitochondrial cytochrome b gene-targeted PCR. International-journal Peer-reviewed

Tatsuro Hifumi, Tetsuya Tanaka, Ichinosuke Suzu, Miho Sato, Kohei Akioka, Chiaki Fujimata, Ryohei Shinkai, Tsutomu Maeda, Kodai Kusakisako, Hiromi Ikadai, Noriaki Miyoshi

Food and waterborne parasitology　34　e00219　2024/03

DOI： 10.1016/j.fawpar.2024.e00219 　

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Alveolar echinococcosis is a zoonotic disease caused by a larval-stage Echinococcus multilocularis infection. Geographical haplotyping targeting the parasite's mitochondrial cytochrome b (cob) gene has been reported for isolates from definitive and intermediate hosts (wild canids and rodents); however, there are limited reports on strain typing for the dead-end host, the horse, which could act as a sentinel for E. multilocularis. Accordingly, we investigated the diversity of E. multilocularis in isolates obtained from slaughtered Japanese and Canadian horses originating from the Iburi and Hidaka regions in Hokkaido and from Alberta, respectively, with PCR and haplogroup analyses targeting cob gene sequences obtained. Seventy horses were diagnosed with alveolar echinococcosis based on histopathology and cob-gene PCR testing. The E. multilocularis detected in these horses was classified as either an Asian (for Hokkaido-raised horses) or a European (for Alberta-raised horses) haplogroup, based on the obtained cob-gene sequence analysis. In addition, haplotype network analysis revealed that E. multilocularis isolated from Hokkaido-raised horses is highly homologous to Kazakhstan isolates, and E. multilocularis isolated from Alberta-raised horses is highly homologous to Austrian isolates. The results of this study suggest that cob-gene-targeted PCR analysis could be useful for the geographical genetic characterization of E. multilocularis isolated from horses.
First report of spotted fever group Rickettsia aeschlimannii in Hyalomma turanicum, Haemaphysalis bispinosa, and Haemaphysalis montgomeryi infesting domestic animals: updates on the epidemiology of tick-borne Rickettsia aeschlimannii. International-journal Peer-reviewed

Abdul Majid, Mashal M Almutairi, Abdulaziz Alouffi, Tetsuya Tanaka, Tsai-Ying Yen, Kun-Hsien Tsai, Abid Ali

Frontiers in microbiology　14　1283814-1283814　2023/12

DOI： 10.3389/fmicb.2023.1283814 　

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Tick-borne Rickettsia spp. have long been known as causative agents for zoonotic diseases. We have previously characterized Rickettsia spp. in different ticks infesting a broad range of hosts in Pakistan; however, knowledge regarding Rickettsia aeschlimannii in Haemaphysalis and Hyalomma ticks is missing. This study aimed to obtain a better understanding about R. aeschlimannii in Pakistan and update the knowledge about its worldwide epidemiology. Among 369 examined domestic animals, 247 (66%) were infested by 872 ticks. Collected ticks were morphologically delineated into three genera, namely, Rhipicephalus, Hyalomma, and Haemaphysalis. Adult females were the most prevalent (number ₌ 376, 43.1%), followed by nymphs (303, 34.74%) and males (193, 22.13%). Overall, genomic DNA samples of 223 tick were isolated and screened for Rickettsia spp. by the amplification of rickettsial gltA, ompA, and ompB partial genes using conventional PCR. Rickettsial DNA was detected in 8 of 223 (3.58%) ticks including nymphs (5 of 122, 4.0%) and adult females (3 of 86, 3.48%). The rickettsial gltA, ompA, and ompB sequences were detected in Hyalomma turanicum (2 nymphs and 1 adult female), Haemaphysalis bispinosa (1 nymph and 1 adult female), and Haemaphysalis montgomeryi (2 nymphs and 1 adult female). These rickettsial sequences showed 99.71-100% identity with R. aeschlimannii and phylogenetically clustered with the same species. None of the tested Rhipicephalus microplus, Hyalomma isaaci, Hyalomma scupense, Rhipicephalus turanicus, Hyalomma anatolicum, Rhipicephalus haemaphysaloides, Rhipicephalus sanguineus, Haemaphysalis cornupunctata, and Haemaphysalis sulcata ticks were found positive for rickettsial DNA. Comprehensive surveillance studies should be adopted to update the knowledge regarding tick-borne zoonotic Rickettsia species, evaluate their risks to humans and livestock, and investigate the unexamined cases of illness after tick bite among livestock holders in the country.
Isolation, identification and antimicrobial susceptibility of the bacteria isolated from Hyalomma dromedarii infesting camels in Al-Jouf province, Saudi Arabia. International-journal Peer-reviewed

Alanoud T Aljasham, Eman M Damra, Nora S Alkahtani, Abdulaziz Alouffi, Waleed S Al Salem, Aljoharah O Alshabanah, Moureq Alotaibi, Tetsuya Tanaka, Abid Ali, Mashal M Almutairi

Frontiers in veterinary science　10　1227908-1227908　2023/12

DOI： 10.3389/fvets.2023.1227908 　

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Ticks are important ectoparasites that transmit various pathogens causing morbidity and mortality in humans and animals. Saudi Arabia faces several challenges that can contribute to the emergence and spread of antimicrobial resistance (AMR) bacteria. These challenges require collaborative efforts to successfully achieve significant control of AMR in the country. The present study aims to isolate bacteria from camels' tick Hyalomma dromedarii in Al-Jouf province to identify and determine these isolates' antimicrobial susceptibilities. Forty-nine ticks were collected from dromedary camels and morphologically classified as H. dromedarii. Ticks were then homogenized and plated individually, which resulted in the isolation of 55 bacteria. The results showed that the bacterial isolates belong to 20 different species. About 71% (n = 39) of the total isolates were identified as Gram-positive bacteria comprised of 11 different species, while 29% (n = 16) of the total isolates were Gram-negative bacteria comprised of 9 different species. The most prevalent isolate within the total samples was Staphylococcus lentus (22.45%, 11/49), followed by Staphylococcus pseudintermedius (18.37%, 9/49) and Sphingomonas paucimobilis (16.33% 8/49). The antimicrobial susceptibility profile of Gram-positive bacteria showed that 100% (n = 31) were resistant to benzylpenicillin; 90.3% (n = 28) were resistant to oxacillin; 58.1% (n = 18) were resistant to clindamycin; 48.4% (n = 15) were resistant to vancomycin. In addition, 32.3% (n = 10) were resistant to trimethoprim/sulfamethoxazole and rifampicin; 25.8% (n = 8) were resistant to erythromycin; 16.1% (n = 5) were resistant to teicoplanin; 6.5% (n = 2) were resistant to tetracycline. All Gram-positive bacteria were 100% susceptible to linezolid, gentamicin, tobramycin, levofloxacin, moxifloxacin, tigecycline, and nitrofurantoin. In antimicrobial susceptibility tests for the Gram-negative bacteria, 57.14% (n = 8) of the identified bacteria were resistant to ampicillin, whereas 50% (n = 7) were resistant to cefoxitin and ceftazidime. About 28.57% (n = 4) of the Gram-negative bacteria were resistant to ceftriaxone, trimethoprim/sulfamethoxazole. In addition, 21.43% (n = 3) were resistant to amoxicillin/clavulanic acid and cephalothin; 14.29% (n = 2) were resistant to cefepime and nitrofurantoin; 7.14% (n = 1) were resistant to piperacillin/tazobactam and tigecycline. However, all Gram-negative bacteria were susceptible to other examined antimicrobials. This is the first study that investigates the role of the hard tick as a potential reservoir for AMR pathogens within our region.
Molecular evidence of Borrelia theileri and closely related Borrelia spp. in hard ticks infesting domestic animals. International-journal Peer-reviewed

Mehran Khan, Mashal M Almutairi, Abdulaziz Alouffi, Tetsuya Tanaka, Shun-Chung Chang, Chien-Chin Chen, Abid Ali

Frontiers in veterinary science　10　1297928-1297928　2023/12

DOI： 10.3389/fvets.2023.1297928 　

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Ticks pose significant threats to hosts by transmitting Borrelia spp., which are grouped into Lyme borreliae, relapsing fever borreliae (RF), and reptiles- and monotremes-associated borreliae. The RF borreliae encompass a group of Borrelia species predominantly transmitted by soft ticks, but some of its members can also be transmitted by hard ticks. Information on the detection and genetic characterization of tick-borne RF borreliae, including Borrelia theileri, is notably rare in Asia, particularly in Pakistan. Herein, we employed molecular techniques to detect borreliae in hard ticks collected from domestic animals in Khyber Pakhtunkhwa, Pakistan. Ticks were subjected to morphological analysis, followed by DNA extraction and PCR amplification of partial fragments of borrelial 16S rRNA and flaB genes. A total of 729 ticks were collected from 264 hosts, with Haemaphysalis cornupunctata (12.9%; 94/729) being the most prevalent, followed by Hyalomma anatolicum (11.7%; 85/729), Rhipicephalus microplus (10.0%; 73/729), Haemaphysalis kashmirensis (9.1%; 66/729), Haemaphysalis bispinosa (8.5%; 62/729), Rhipicephalus sanguineus (8%; 58/729), Haemaphysalis montgomeryi (6.2%; 45/729), Rhipicephalus turanicus (5.5%; 40/729), Hyalomma dromedarii and Ixodes kashmirensis (4.4%; 32/729 each), Rhipicephalus haemaphysaloides (4.1%; 30/729), Haemaphysalis sulcata and Hyalomma scupense (3.8%; 28/729 each), Haemaphysalis danieli (2.9%; 21/729), Hyalomma kumari (2.6%; 19/729), and Hyalomma isaaci (2.2%; 16/729). Based on 16S rRNA detection of Borrelia spp., only R. turanicus yielded positive results, resulting in an overall infection rate of 0.3% (2/160), while using flaB-based detection, four tick species including R. microplus, R. turanicus, Ha. sulcata, and Ha. cornupunctata showed positive results, yielding an overall infection rate of 6.9% (11/160). The amplified DNA fragments of borrelial 16S rRNA and flaB in R. turanicus from goats shared maximum identities of 100 and 99.40% with Borrelia theileri, respectively. Amplified borrelial flaB fragments in R. microplus from cows and sheep displayed 100% identity with B. theileri, while flaB fragments in Ha. cornupunctata and Ha. sulcata from goats revealed identities of 99.32 and 99.75% with undetermined RF Borrelia spp., respectively. Phylogenetic analysis revealed clustering of B. theileri from R. microplus and R. turanicus with the same species, while Borrelia spp. from Ha. cornupunctata and Ha. sulcata with undetermined RF Borrelia spp. Notably, this research marks the first documentation of B. theileri in R. turanicus and the identification of RF Borrelia spp. in Ha. cornupunctata and Ha. sulcata.
First Molecular-Based Confirmation of Dermacentor marginatus and Associated Rickettsia raoultii and Anaplasma marginale in the Hindu Kush Mountain Range. International-journal Peer-reviewed

Iftikhar Ahmad, Shafi Ullah, Abdulaziz Alouffi, Mashal M Almutairi, Muhammad Numan, Tetsuya Tanaka, Shun-Chung Chang, Chien-Chin Chen, Abid Ali

Animals : an open access journal from MDPI　13　(23)　2023/11/28

DOI： 10.3390/ani13233686 　

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Ticks of the genus Dermacentor Koch, 1844 (Acari: Ixodidae) are poorly known systematically due to their habitation in harsh topographic environments and high mountains. Dermacentor ticks are diversely distributed in the Palearctic, Nearctic, and Oriental regions. There is no available information on the occurrence of Dermacentor marginatus in Pakistan; thus, the current investigation aimed the first morphological and molecular confirmation of this species and associated Anaplasma marginale and Rickettsia raoultii. Ticks were collected from goats (Capra hircus) and morphologically identified. Genomic DNA was extracted from 18/26 (69.23%) tick specimens, including 11 males and 7 females (1 unfed and 6 fed females). Extracted DNA was subjected to PCR for the amplification of genetic markers like 16S rDNA and cox1 for ticks, 16S rDNA for Anaplasma spp., and gltA and ompB for Rickettsia spp. A total of 26 D. marginatus ticks composed of 19 males (73.07%) and 7 females (26.9%) [1 (3.84%) unfed and 6 (23.07%) fed females] were collected from goats. According to amplicons via BLAST analysis, the 16S rDNA sequence showed 97.28-98.85% identity and the cox1 sequence showed 95.82-98.03% identity with D. marginatus. Additionally, the 16S rDNA sequence for Anaplasma sp. was detected in D. marginatus that showed 100% identity with Anaplasma marginale. Rickettsial gltA and ompB sequences for Rickettsia sp. showed 100% identity with Rickettsia raoultii. In phylogenetic analysis, ticks' 16S rDNA and cox1 sequences clustered with the same species. In phylogenetic analysis, A. marginale based on 16 rDNA clustered with A. marginale, while gltA and ompB sequences clustered with R. raoultii. This is the first study on the genetic characterization of D. marginatus and associated A. marginale and R. raoultii in Pakistan. The northern areas of Pakistan, which need to be explored in terms of ticks and associated pathogens due to their zoonotic threats, have been neglected due to the inaccessible climatic conditions.
Molecular Survey of Rickettsia raoultii in Ticks Infesting Livestock from Pakistan with Notes on Pathogen Distribution in Palearctic and Oriental Regions. International-journal Peer-reviewed

Shehla Shehla, Mashal M Almutairi, Abdulaziz Alouffi, Tetsuya Tanaka, Shun-Chung Chang, Chien-Chin Chen, Abid Ali

Veterinary sciences　10　(11)　2023/10/29

DOI： 10.3390/vetsci10110636 　

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Ticks are hematophagous ectoparasites that transmit different pathogens such as Rickettsia spp. to domestic and wild animals as well as humans. Genetic characterizations of Rickettsia spp. from different regions of Pakistan are mostly based on one or two genetic markers and are confined to small sampling areas and limited host ranges. Therefore, this study aimed to molecularly screen and genetically characterize Rickettsia spp. in various tick species infesting camels, sheep, and goats. All the collected tick specimens were morphologically identified, and randomly selected tick species (148) were screened molecularly for the detection of Rickettsia spp. by amplifying three rickettsial DNA fragments, namely, the citrate-synthase gene (gltA), outer-membrane protein A (ompA), and outer-membrane protein B (ompB). After examining 261 hosts, 161 (61.7%) hosts were found infested by 564 ticks, including 287 (50.9%) nymphs, 171 (30.3%) females, and 106 (18.8%) males in five districts (Kohat, Dera Ismail Khan, Lower Dir, Bajaur, and Mansehra). The highest occurrence was noted for Hyalomma dromedarii (number = 72, 12.8%), followed by Haemaphysalis sulcata (n = 70, 12.4%), Rhipicephalus turanicus (n = 64, 11.3%), Rhipicephalus microplus (n = 55, 9.7%), Haemaphysalis cornupunctata (n = 49, 8.7%), Hyalomma turanicum (n = 48, 8.5%), Hyalomma isaaci (n = 45, 8.0%), Haemaphysalis montgomeryi (n = 44, 7.8%), Hyalomma anatolicum (n = 42, 7.5%), Haemaphysalis bispinosa (n = 38, 6.7%), and Rhipicephalus haemaphysaloides (n = 37, 6.6%). A subset of 148 ticks were tested, in which eight (5.4%) ticks, including four Hy. turanicum, two Ha. cornupunctata, one Ha. montgomeryi, and one Ha. bispinosa, were found positive for Rickettsia sp. The gltA, ompA, and ompB sequences revealed 100% identity and were phylogenetically clustered with Rickettsia raoultii reported in China, Russia, USA, Turkey, Denmark, Austria, Italy, and France. Additionally, various reports on R. raoultii from Palearctic and Oriental regions were summarized in this study. To the best of our knowledge, this is the first report regarding genetic characterization and phylogenetic analysis of R. raoultii from Pakistan. Further studies to investigate the association between Rickettsia spp. and ticks should be encouraged to apprise effective management of zoonotic consequences.
Argas persicus and Carios vespertilionis Ticks Infesting Ducks, Domestic Fowls and Bats in Pakistan: First Report on Molecular Survey and Phylogenetic Position of Borrelia anserina. International-journal Peer-reviewed

Hafsa Zahid, Abdulaziz Alouffi, Mashal M Almutairi, Muhammad Ateeq, Tetsuya Tanaka, Shun-Chung Chang, Chien-Chin Chen, Abid Ali

Veterinary sciences　10　(10)　2023/10/20

DOI： 10.3390/vetsci10100628 　

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Argasid ticks have the vectorial potential for transmitting disease-causing pathogens to avian hosts, resulting in economic losses that may not be fully estimated. Borrelia species are the responsible agents of borreliosis in poultry, animals and humans. Our previous studies have reported a high prevalence of Argas persicus infesting domestic fowls in Khyber Pakhtunkhwa (KP), Pakistan. However, molecular screening and genetic characterization of Borrelia spp. in A. persicus have been neglected in Pakistan. In this study, we focused on the molecular epidemiology and genetic characterization of Borrelia spp. associated with A. persicus ticks infesting domestic fowls and ducks, and Carios vespertilionis infesting bats in selected districts of KP. Overall, 1818 ticks, including females (415; 23%), males (345; 19%), nymphs (475; 26%) and larvae (583; 32%), were collected from 27 locations in nine districts (Peshawar, Mardan, Swabi, Charsadda, Chitral, Lakki Marwat, Bannu, Bajaur and Hangu) from domestic fowls, ducks and their shelters, and bats. A subset of 197 ticks was selected for DNA extraction and PCR to amplify fragments of the cytochrome c oxidase (cox) gene for ticks and flagellin B (flaB) for the detection and genetic characterization of associated Borrelia spp. Among these, only Borrelia anserina DNA was detected in 40 ticks (27.2%) of different life stages, where highest prevalence was found in female ticks (18; 45%), followed by nymphs (12; 30%), larvae (7; 17.5%) and males (3; 7.5%). Tick infestation in shelters (1081; 77%) was higher than on hosts (323; 23%). The resultant cox amplicons of A. persicus showed 100% identity with the same species reported from Pakistan, China, Iran, Kenya, Kazakhstan, Algeria and Egypt and C. vespertilionis show 100% identity with the species reported from Pakistan, China, Japan, Kenya, Vietnam, Spain, Netherlands, the United Kingdom and Hungry, and clustered with the aforementioned species in the phylogenetic tree. The obtained Borrelia sequences showed 100% identity with B. anserina and revealed a close resemblance to the relapsing fever group and clustered in a monophyletic clade with B. anserina from India, Iran and Brazil in a phylogenetic tree. These results establish the first molecular characterization of B. anserina in A. persicus infesting domestic fowls and ducks in the region, as well as their shelters. To effectively control zoonotic consequences, country-wide surveillance research should be encouraged to screen soft ticks infesting various birds for associated pathogens.
First Detection of Theileria sinensis-like and Anaplasma capra in Ixodes kashmiricus: With Notes on cox1-Based Phylogenetic Position and New Locality Records. International-journal Peer-reviewed

Muhammad Numan, Abdulaziz Alouffi, Mashal M Almutairi, Tetsuya Tanaka, Haroon Ahmed, Haroon Akbar, Muhammad Imran Rashid, Kun-Hsien Tsai, Abid Ali

Animals : an open access journal from MDPI　13　(20)　2023/10/17

DOI： 10.3390/ani13203232 　

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Ixodes ticks transmit Theileria and Anaplasma species to a wide range of animals. The spreading of ticks and tick-borne pathogens has been attributed to transhumant herds, and research on these uninvestigated issues has been neglected in many countries, including Pakistan. Recently, we used internal transcribed spacer (ITS) and 16S ribosomal DNA partial sequences to genetically characterize Ixodes kashmiricus ticks and their associated Rickettsia spp. However, the data on its cox1 sequence and associated Theileria spp. and Anaplasma spp. are missing. This study aimed to genetically characterize I. kashmiricus based on the cox1 sequence and their associated Theileria spp. and Anaplasma spp. The I. kashmiricus ticks were collected from small ruminants: sheep (Ovis aries) and goats (Capra hircus) of transhumant herds in district Shangla, Dir Upper and Chitral, Khyber Pakhtunkhwa (KP), Pakistan. Out of 129 examined hosts, 94 (72.87%) (56 sheep and 38 goats) were infested by 352 ticks, including adult females (175; 49.7%) followed by nymphs (115; 32.7%) and males (62; 17.6%). For molecular analyses, 121 ticks were subjected to DNA isolation and PCR for the amplification of the cox1 sequence for I. kashmiricus, 18S rDNA for Theileria spp. and 16S rDNA sequences for Anaplasma spp. The obtained cox1 sequence showed 89.29%, 88.78%, and 88.71% identity with Ixodes scapularis, Ixodes gibbosus, and Ixodes apronophorus, respectively. Phylogenetically, the present cox1 sequence clustered with the Ixodes ricinus complex. Additionally, the 18S rDNA sequence showed 98.11% maximum identity with Theileria cf. sinensis and 97.99% identity with Theileria sinensis. Phylogenetically, Theileria spp. clustered with the T. cf. sinensis and T. sinensis. In the case of Anaplasma spp., the 16S rDNA sequence showed 100% identity with Anaplasma capra and phylogenetically clustered with the A. capra. PCR-based DNA detection targeting the amplification of groEL and flaB sequences of Coxiella spp. and Borrelia spp., respectively, was unsuccessful. This is the first phylogenetic report based on cox1 and new locality records of I. kashmiricus, and the associated T. sinensis-like and A. capra. Significant tick surveillance studies are needed in order to determine the epidemiology of Ixodes ticks and their associated pathogens.
Molecular Detection of Rickettsia hoogstraalii in Hyalomma anatolicum and Haemaphysalis sulcata: Updated Knowledge on the Epidemiology of Tick-Borne Rickettsia hoogstraalii. International-journal Peer-reviewed

Aneela Aneela, Mashal M Almutairi, Abdulaziz Alouffi, Haroon Ahmed, Tetsuya Tanaka, Itabajara da Silva Vaz Junior, Shun-Chung Chang, Chien-Chin Chen, Abid Ali

Veterinary sciences　10　(10)　2023/10/04

DOI： 10.3390/vetsci10100605 　

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Ticks are hematophagous ectoparasites that transmit pathogens to animals and humans. Updated knowledge regarding the global epidemiology of tick-borne Rickettsia hoogstraalii is dispersed, and its molecular detection and genetic characterization are missing in Pakistan. The current study objectives were to molecularly detect and genetically characterize Rickettsia species, especially R. hoogstraalii, in hard ticks infesting livestock in Pakistan, and to provide updated knowledge regarding their global epidemiology. Ticks were collected from livestock, including goats, sheep, and cattle, in six districts of Khyber Pakhtunkhwa (KP) Pakistan. Overall, 183 hosts were examined, of which 134 (73.2%), including goats (number = 39/54, 72.2%), sheep (23/40, 57.5%), and cattle (71/89, 80%) were infested by 823 ticks. The most prevalent tick species was Rhipicephalus microplus (number = 283, 34.3%), followed by Hyalomma anatolicum (223, 27.0%), Rhipicephalus turanicus (122, 14.8%), Haemaphysalis sulcata (104, 12.6%), Haemaphysalis montgomeryi (66, 8.0%), and Haemaphysalis bispinosa (25, 3.03%). A subset of 210 ticks was selected and screened for Rickettsia spp. using PCR-based amplification and subsequent sequencing of rickettsial gltA and ompB fragments. The overall occurrence rate of R. hoogstraalii was 4.3% (number = 9/210). The DNA of Rickettsia was detected in Hy. anatolicum (3/35, 8.5%) and Ha. sulcata (6/49, 12.2%). However, no rickettsial DNA was detected in Rh. microplus (35), Rh. turanicus (35), Ha. montgomeryi (42), and Ha. bispinosa (14). The gltA and ompB fragments showed 99-100% identity with R. hoogstraalii and clustered phylogenetically with the corresponding species from Pakistan, Italy, Georgia, and China. R. hoogstraalii was genetically characterized for the first time in Pakistan and Hy. anatolicum globally. Further studies should be encouraged to determine the role of ticks in the maintenance and transmission of R. hoogstraalii in different hosts.
Association of SFG Rickettsia massiliae and Candidatus Rickettsia shennongii with Different Hard Ticks Infesting Livestock Hosts. International-journal Peer-reviewed

Shehla Shehla, Farman Ullah, Abdulaziz Alouffi, Mashal M Almutairi, Zaibullah Khan, Tetsuya Tanaka, Marcelo B Labruna, Kun-Hsien Tsai, Abid Ali

Pathogens (Basel, Switzerland)　12　(9)　2023/08/24

DOI： 10.3390/pathogens12091080 　

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Ixodid ticks are responsible for the transmission of various intracellular bacteria, such as the Rickettsia species. Little Information is available about the genetic characterization and epidemiology of Rickettsia spp. The current study was designed to assess the tick species infesting various livestock hosts and the associated Rickettsia spp. in Pakistan. Ticks were collected from different livestock hosts (equids, cattle, buffaloes, sheep, goats, and camels); morphologically identified; and screened for the genetic characterization of Rickettsia spp. by the amplification of partial fragments of the gltA, ompA and ompB genes. Altogether, 707 ticks were collected from 373 infested hosts out of 575 observed hosts. The infested hosts comprised 105 cattle, 71 buffaloes, 70 sheep, 60 goats, 34 camels, and 33 equids. The overall occurrence of Rickettsia spp. was 7.6% (25/330) in the tested ticks. Rickettsia DNA was detected in Rhipicephalus haemaphysaloides (9/50, 18.0%), followed by Rhipicephalus turanicus (13/99, 13.1%), Haemaphysalis cornupunctata (1/18, 5.5%), and Rhipicephalus microplus (2/49, 4.1%); however, no rickettsial DNA was detected in Hyalomma anatolicum (71), Hyalomma dromedarii (35), and Haemaphysalis sulcata (8). Two Rickettsia agents were identified based on partial gltA, ompA, and ompB DNA sequences. The Rickettsia species detected in Rh. haemaphysaloides, Rh. turanicus, and Rh. microplus showed 99-100% identity with Rickettsia sp. and Candidatus Rickettsia shennongii, and in the phylogenetic trees clustered with the corresponding Rickettsia spp. The Rickettsia species detected in Rh. haemaphysaloides, Rh. turanicus, Rh. microplus, and Ha. cornupunctata showed 100% identity with R. massiliae, and in the phylogenetic trees it was clustered with the same species. Candidatus R. shennongii was characterized for the first time in Rh. haemaphysaloides, Rh. turanicus, and Rh. microplus. The presence of SFG Rickettsia spp., including the human pathogen R. massiliae, indicates a zoonotic risk in the study region, thus stressing the need for regular surveillance.
RNA activation in ticks. International-journal Peer-reviewed

Kofi Dadzie Kwofie, Emmanuel Pacia Hernandez, Anisuzzaman, Hayato Kawada, Yuki Koike, Sana Sasaki, Takahiro Inoue, Kei Jimbo, Fusako Mikami, Danielle Ladzekpo, Rika Umemiya-Shirafuji, Kayoko Yamaji, Tetsuya Tanaka, Makoto Matsubayashi, Md Abdul Alim, Samuel Kweku Dadzie, Shiroh Iwanaga, Naotoshi Tsuji, Takeshi Hatta

Scientific reports　13　(1)　9341-9341　2023/06/08

DOI： 10.1038/s41598-023-36523-4 　

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RNA activation (RNAa) is a burgeoning area of research in which double-stranded RNAs (dsRNAs) or small activating RNAs mediate the upregulation of specific genes by targeting the promoter sequence and/or AU-rich elements in the 3'- untranslated region (3'-UTR) of mRNA molecules. So far, studies on the phenomenon have been limited to mammals, plants, bacteria, Caenorhabditis elegans, and recently, Aedes aegypti. However, it is yet to be applied in other arthropods, including ticks, despite the ubiquitous presence of argonaute 2 protein, which is an indispensable requirement for the formation of RNA-induced transcriptional activation complex to enable a dsRNA-mediated gene activation. In this study, we demonstrated for the first time the possible presence of RNAa phenomenon in the tick vector, Haemaphysalis longicornis (Asian longhorned tick). We targeted the 3'-UTR of a novel endochitinase-like gene (HlemCHT) identified previously in H. longicornis eggs for dsRNA-mediated gene activation. Our results showed an increased gene expression in eggs of H. longicornis endochitinase-dsRNA-injected (dsHlemCHT) ticks on day-13 post-oviposition. Furthermore, we observed that eggs of dsHlemCHT ticks exhibited relatively early egg development and hatching, suggesting a dsRNA-mediated activation of the HlemCHT gene in the eggs. This is the first attempt to provide evidence of RNAa in ticks. Although further studies are required to elucidate the detailed mechanism by which RNAa occurs in ticks, the outcome of this study provides new opportunities for the use of RNAa as a gene overexpression tool in future studies on tick biology, to reduce the global burden of ticks and tick-borne diseases.
Rapid detection of alveolar echinococcosis in hepatic nodules of horses by recombinase polymerase amplification assay. International-journal Peer-reviewed

Tatsuro Hifumi, Tetsuya Tanaka, Miho Sato, Kohei Akioka, Chiaki Fujimata, Noriaki Miyoshi

Veterinary and animal science　20　100291-100291　2023/06

DOI： 10.1016/j.vas.2023.100291 　

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Alveolar echinococcosis in slaughtered horses remains a public health issue. This study aimed to develop a Recombinase polymerase amplification (RPA) assay targeting the mitochondrial NADH dehydrogenase subunit 5 (Nad5) gene of Echinococcus multilocularis for the rapid detection of equine alveolar echinococcosis. Thirty-six hepatic solid nodules obtained from each horse (n = 36) were evaluated based on histopathological examination and Nad5-targeted PCR and then submitted to the RPA assay. The results of the developed RPA assay were 94.4% consistent with those of Nad5 PCR and Cohen's kappa coefficient value was 0.89 statistically, indicating high agreement. In addition, the RPA assay using the plasmid samples was one hundredfold more sensitive than PCR testing. Consequently, these results suggest that the performance of the RPA assay developed in this study is equal to that of conventional PCR testing.
Phylogenetic Position of Haemaphysalis kashmirensis and Haemaphysalis cornupunctata, with Notes on Rickettsia spp. International-journal Peer-reviewed

Shah Masood Khan, Mehran Khan, Abdulaziz Alouffi, Mashal M Almutairi, Muhmmad Numan, Shafi Ullah, Muhammad Kashif Obaid, Zia Ul Islam, Haroon Ahmed, Tetsuya Tanaka, Abid Ali

Genes　14　(2)　2023/01/30

DOI： 10.3390/genes14020360 　

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Despite high diversity in the Oriental region, ticks of the genus Haemaphysalis have been neglected regarding their genetic data and vector potential. This study aimed to genetically characterize three species of the genus Haemaphysalis: Haemaphysalis cornupunctata, Haemaphysalis kashmirensis, and Haemaphysalis montgomeryi infesting goats and sheep, and Rickettsia spp. associated with these tick species in the Hindu Kush Himalayan range of Pakistan. Altogether, 834 ticks were collected by examining 120 hosts including goats (64/120, 53.3%) and sheep (56/120, 46.6%), in which 86 (71.6%) hosts were found to be tick-infested. The morphologically identified ticks were subjected to DNA extraction and PCR for the amplification of partial 16S rDNA and cox fragments. Rickettsia spp. associated with the collected ticks were detected through the amplification of gltA, ompA and ompB partial fragments. The 16S rDNA of H. cornupunctata and H. montgomeryi showed a maximum identity of 100% with the sequences of the same species, whereas the 16S rDNA of H. kashmirensis showed the highest identity of 93-95% with Haemaphysalis sulcata. The cox sequence of H. montgomeryi displayed 100% identity with the same species. In comparison, the cox sequences of H. cornupunctata and H. kashmirensis showed maximum identities of 87.65-89.22% with Haemaphysalis punctata and 89.34% with H. sulcata, respectively. The gltA sequence of Rickettsia sp. from H. kashmirensis showed the highest identity of 97.89% with Rickettsia conorii subsp. raoultii, while the ompA and ompB fragments from the same DNA samples revealed 100% and 98.16% identity with Rickettsia sp. and "Candidatus Rickettsia longicornii", respectively. Another gltA sequence amplified from H. montgomeryi ticks showed 100% identity with Rickettsia hoogstraalii, while the attempts to amplify ompA and ompB for R. hoogstraalii were unsuccessful. In the phylogenetic tree, the 16S rDNA of H. cornupunctata clustered with the corresponding species while its cox clustered with H. punctata. Both 16S rDNA and cox sequences of H. kashmirensis clustered with H. sulcata. The gltA sequence of Rickettsia sp. was clustered individually in the spotted fever (SF) group of Rickettsia, while the gltA sequence of R. hoogstraalii was clustered with the same species in the transition group of Rickettsia. In the SF group, the rickettsial ompA and ompB sequence clustered with undetermined Rickettsia sp. and "Candidatus Rickettsia longicornii", respectively. This is the earliest study regarding the genetic characterization of H. kashmirensis. This study indicated that ticks belong to the genus Haemaphysalis have the potential of harboring and/or transmitting Rickettsia spp. in the region.
Localization of secreted ferritin (FER2) in the embryos of the tick Haemaphysalis longicornis. International-journal Peer-reviewed

Emmanuel Pacia Hernandez, Kei Shimazaki, Hiroko Niihara, Rika Umemiya-Shirafuji, Kozo Fujisaki, Tetsuya Tanaka

Parasites & vectors　16　(1)　42-42　2023/01/30

DOI： 10.1186/s13071-023-05669-5 　

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Despite the absence of a blood meal, embryogenesis involves many processes that require nutrients and other essential elements, including iron. Due to the lack of an external source of these nutrients, these requirements are acquired maternally. Because of the toxic nature of iron, they are transferred through iron transport molecules such as secreted ferritin (FER2). Here we tried to follow the trail of the FER2 through indirect immunofluorescence, and we observed an apparent shift of FER2 from the germ layer at the early part of development to the appendages during the late stage of embryogenesis. FER2 is also found in the middle part of the legs of the embryo. The apparent movement not only sheds light on iron processing events during embryogenesis but also indirectly guides organogenesis in the tick.
First record of Alectorobius coniceps (Ixodoidea: Argasidae) and Dermacentor sp. (Ixodoidea: Ixodidae) in Pakistan. International-journal Peer-reviewed

Abid Ali, Mehran Khan, Zafar Ullah, Muhammad Numan, Kun-Hsien Tsai, Abdulaziz Alouffi, Mashal M Almutairi, Tetsuya Tanaka

Frontiers in veterinary science　10　1326734-1326734　2023

DOI： 10.3389/fvets.2023.1326734 　

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Alectorobius species are soft ticks primarily infesting birds, such as swallows, while Dermacentor species are hard ticks mainly infesting mammals, such as small ruminants. This study for the first time reported on the morphological and molecular bases of two tick species, namely A. coniceps and a Dermacentor sp. in Pakistan. The former species was examined in swallows' nests in Khyber Pakhtunkhwa province, while the latter species was examined in small ruminants in Balochistan province. In total, 25 ticks were collected, with 14 ticks morphologically identified as A. coniceps (males = 9 and females = 5) and 11 ticks identified as Dermacentor sp. (males = 7 and females = 4). Following morphological identification, molecular identification was gained by obtaining 16S rDNA and cox1 sequences for these ticks. The BLAST results for the 16S rDNA and cox1 sequences from A. coniceps shared a maximum identity of 97.46% and 96.49% with the same species from Malta. The BLAST analysis of the 16S rDNA and cox1 sequences from Dermacentor sp. showed maximum identities of 98.42% and 97.45% with Dermacentor pavlovskyi from China. The phylogenetic analysis based on 16S rDNA and cox1 of A. coniceps showed a close evolutionary relationship with the same species. The case of Dermacentor sp., based on 16S DNA and cox1, indicated a close evolutionary relationship with Dermacentor pavlovskyi from China.
First report on detection of Hepatozoon ayorgbor in Rhipicephalus haemaphysaloides and Hepatozoon colubri in Haemaphysalis sulcata and Hyalomma anatolicum: risks of spillover of Hepatozoon spp. from wildlife to domestic animals. International-journal Peer-reviewed

Hadia Tila, Mehran Khan, Mashal M Almutairi, Abdulaziz Alouffi, Haroon Ahmed, Tetsuya Tanaka, Kun-Hsien Tsai, Abid Ali

Frontiers in veterinary science　10　1255482-1255482　2023

DOI： 10.3389/fvets.2023.1255482 　

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This study aimed to detect Hepatozoon spp. in ticks infesting asymptomatic domestic animals and to provide insight into their potential spillover from wild to domestic animals. In total, 537 tick specimens were collected in Khyber Pakhtunkhwa, Pakistan, and morphologically identified. The most prevalent tick species was Haemaphysalis cornupunctata (69; 12.8%), followed by Haemaphysalis kashmirensis (62; 11.5%), Rhipicephalus microplus (58; 10.8%), Haemaphysalis montgomeryi (51; 9.5%), Rhipicephalus sanguineus (49; 9.1%), each Haemaphysalis bispinosa and Haemaphysalis sulcata (43; 8.0%), each Hyalomma anatolicum and Rhipicephalus turanicus (37; 6.9%), Rhipicephalus haemaphysaloides (33; 6.1%) Hyalomma scupense (30; 5.6%), and Hyalomma isaaci (25; 4.7%). The extracted DNA from a subset of each tick species was subjected to PCR to amplify 18S rRNA fragments of Hepatozoon spp. By BLAST analysis, the Hepatozoon sp. detected in Hy. anatolicum infesting cows and in Ha. sulcata infesting sheep showed 99.7% maximum identity with Hepatozoon colubri. Similarly, the Hepatozoon sp. detected in R. haemaphysaloides infesting goats shared 99.49% maximum identity with Hepatozoon ayorgbor, and the Hepatozoon sp. detected in R. sanguineus infesting dogs exhibited 99.7% identity with Hepatozoon canis. Having an overall infection rate (9.3%; 16/172), the highest infection rate was recorded for each H. canis, and H. colubri (3.5%; 6/172), followed by H. ayorgbor (2.3%; 4/172). In the phylogenetic tree, H. colubri clustered with corresponding species from Iran, H. ayorgbor clustered with the same species from Croatia, Ghana, and Portugal, and H. canis clustered with the conspecifics from Iran, Israel, Romania, and Zambia. Regarding the potential spillover of Hepatozoon spp. from wildlife through ticks, free ranging animals was at higher risk compared to confined animals (RR = 3.05), animals consuming food from wildlife habitats were at higher risk compared to those consuming domestic food (RR = 3.06), and animals residing in farm buildings located in wildlife habitats were at higher risk compared to those residing in farm buildings located in villages (RR = 3.28). In addition to the first report on H. canis in R. sanguineus in Pakistan, this is the earliest data showing H. ayorgbor in R. haemaphysaloides and H. colubri in Ha. sulcata and Hy. anatolicum. These preliminary findings suggest a potential spillover of Hepatozoon spp. from wild to domestic animals via ticks under certain risk factors.
First report on tick-borne pathogens detected in ticks infesting stray dogs near butcher shops. International-journal Peer-reviewed

Abid Ali, Shafi Ullah, Muhammad Numan, Mashal M Almutairi, Abdulaziz Alouffi, Tetsuya Tanaka

Frontiers in veterinary science　10　1246871-1246871　2023

DOI： 10.3389/fvets.2023.1246871 　

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Public health is a major concern for several developing countries due to infectious agents transmitted by hematophagous arthropods such as ticks. Health risks due to infectious agents transmitted by ticks infesting butcher-associated stray dogs (BASDs) in urban and peri-urban regions have been neglected in several developing countries. To the best of the authors' knowledge, this is the first study assessing public health risks due to ticks infesting BASDs in Pakistan's urban and peri-urban areas. A total of 575 ticks (390 from symptomatic and 183 from asymptomatic BASDs) were collected from 117 BASDs (63 symptomatic and 54 asymptomatic); the ticks belonged to 4 hard tick species. A subset of each tick species' extracted DNA was subjected to polymerase chain reaction (PCR) to amplify the 16S rDNA and cox1 sequences of the reported tick species, as well as bacterial and protozoal agents. The ticks' 16S rDNA and cox1 sequences showed 99-100% identities, and they were clustered with the sequence of corresponding species from Pakistan and other countries in phylogenetic trees. Among the screened 271 ticks' DNA samples, Anaplasma spp. was detected in 54/271 (19.92%) samples, followed by Ehrlichia spp. (n = 40/271, 14.76%), Rickettsia spp. (n = 33/271, 12.17%), Coxiella spp. (n = 23/271, 4.48%), and Hepatozoon canis (n = 9/271, 3.32%). The obtained sequences and phylogenetic analyzes revealed that the pathogens detected in ticks were Ehrlichia minasensis, Ehrlichia sp., Hepatozoon canis, Coxiella burnetii, Coxiella sp., Anaplasma capra, Anaplasma platys, Anaplasma sp., Rickettsia massiliae, "Candidatus Rickettsia shennongii" and Rickettsia aeschlimannii. Tick-borne pathogens such as E. minasensis, H. canis, A. capra, A. platys, and R. aeschlimannii, were detected based on the DNA for the first time in Pakistan. This is the first report on public health risks due to ticks infesting BASDs. These results not only provided insights into the occurrence of novel tick-borne pathogens in the region but also revealed initial evidence of zoonotic threats to both public health and domestic life.
Molecular detection of Coxiella spp. in ticks (Ixodidae and Argasidae) infesting domestic and wild animals: with notes on the epidemiology of tick-borne Coxiella burnetii in Asia. International-journal Peer-reviewed

Abid Ali, Muhammad Kashif Obaid, Mashal M Almutairi, Abdulaziz Alouffi, Muhammad Numan, Shafi Ullah, Gauhar Rehman, Zia Ul Islam, Sher Bahadar Khan, Tetsuya Tanaka

Frontiers in microbiology　14　1229950-1229950　2023

DOI： 10.3389/fmicb.2023.1229950 　

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Tick-borne Coxiella spp. are emerging in novel regions infecting different hosts, but information regarding their occurrence is limited. The purpose of this study was the molecular screening of Coxiella spp. in various ticks infesting goats, sheep, camels, cattle, wild mice, and domestic fowls (Gallus gallus domesticus) in various districts of Khyber Pakhtunkhwa, Pakistan. Morphologically identified tick species were confirmed by obtaining their cox1 sequences and were molecularly screened for Coxiella spp. by sequencing GroEL fragments. Almost 345 out of 678 (50.9%) hosts were infested by nine tick species. Regarding the age groups, the hosts having an age >3 years were highly infested (192/345, 55.6%), while gender-wise infestation was higher in female hosts (237/345, 68.7%). In collected ticks, the nymphs were outnumbered (613/1,119, 54.8%), followed by adult females (293/1,119, 26.2%) and males (213/1,119, 19.7%). A total of 227 ticks were processed for molecular identification and detection of Coxiella spp. The obtained cox1 sequences of nine tick species such as Hyalomma dromedarii, Hyalomma anatolicum, Haemaphysalis cornupunctata, Haemaphysalis bispinosa, Haemaphysalis danieli, Haemaphysalis montgomeryi, Rhipicephalus haemaphysaloides, Rhipicephalus microplus, and Argas persicus showed maximum identities between 99.6% and 100% with the same species and in the phylogenetic tree, clustered to the corresponding species. All the tick species except Ha. danieli and R. microplus were found positive for Coxiella spp. (40/227, 17.6%), including Coxiella burnetii (15/40, 6.7%), Coxiella endosymbionts (14/40, 6.3%), and different Coxiella spp. (11/40, 4.9%). By the BLAST results, the GroEL fragments of Coxiella spp. showed maximum identity to C. burnetii, Coxiella endosymbionts, and Coxiella sp., and phylogenetically clustered to the corresponding species. This is the first comprehensive report regarding the genetic characterization of Coxiella spp. in Pakistan's ticks infesting domestic and wild hosts. Proper surveillance and management measures should be undertaken to avoid health risks.
Molecular detection of Bartonella and Borrelia in pet dogs in Metro Manila and Laguna, Philippines. International-journal Peer-reviewed

Chae Eun Lee, Jeong Hee Ikeda, Mikaella Andrea M Manongdo, Dan Rica T Romerosa, Kristina Andrea C Sandalo-De Ramos, Tetsuya Tanaka, Remil L Galay

Veterinary world　16　(7)　1546-1551　2023

DOI： 10.14202/vetworld.2023.1546-1551 　

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BACKGROUND AND AIM: Bartonella and Borrelia are zoonotic vector-borne pathogens that can infect dogs and humans. Data on Bartonella and Borrelia in dogs in the Philippines are lacking. This study was conducted to validate previous reports and further investigate the occurrence of Bartonella and Borrelia spp. in cities of Metro Manila. MATERIALS AND METHODS: A total of 182 canine blood samples were acquired with DNA using a commercial extraction kit from selected veterinary clinics in the cities of Metro Manila and Laguna, Philippines. The mammalian actin was amplified through polymerase chain reaction (PCR), followed by PCR assays targeting Bartonella gltA and Borrelia flaB. Further PCR assays targeting 16S of Borrelia and ospA and ospC of Borrelia burgdorferi were performed for those that showed flaB bands. RESULTS: A positive band for gltA of Bartonella was observed in 9 (4.95%) samples, whereas a positive band for flaB of Borrelia was observed in 15 (8.24%) samples. Subsequent PCR assays for other genes of Borrelia were negative. CONCLUSION: These results confirmed the presence of Bartonella and warranted further investigation for the possible presence of other Borrelia species.
Molecular detection and characterization of Anaplasma marginale infecting cattle, buffalo, and camel populations in southern Egypt. International-journal Peer-reviewed

Hassan Y A H Mahmoud, Alsagher O Ali, Tetsuya Tanaka

Frontiers in veterinary science　10　1169323-1169323　2023

DOI： 10.3389/fvets.2023.1169323 　

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Anaplasmosis is a severe tickborne disease of ruminants caused by Anaplasma marginale. A. marginale is distributed worldwide and attacks erythrocytes, resulting in an increased body temperature, anemia, jaundice, abortion, and, in some cases, death. Animals infected with this pathogen become lifelong carriers. In this study, we aimed to detect and characterize A. marginale isolated from cattle, buffalo, and camel populations using novel molecular techniques in southern Egypt. In total, 250 samples (from 100 cattle, 75 water buffaloes, and 75 camels) were analyzed by PCR for the presence of Anaplasmataceae, specifically A. marginale. The animals varied in breed, age, and gender, with most showing no signs of severe disease. By species, A. marginale was found in 61 out of 100 (61%) cattle, 9 out of 75 (12%) buffaloes, and only 5 out of 75 (6.66%) camels. All A. marginale-positive samples were examined for the heat-shock protein groEL gene and, additionally, for major surface protein 4 (msp4) and major surface protein 5 (msp5) genes to enhance specificity. Phylogenetic analysis of A. marginale targeted three genes (groEL, msp4, and msp5). This study provides the first report on using three genes for A. marginale detection in Camelus dromedarius in southern Egypt and generated new phylogenetic data for A. marginale infections in camels. A. marginale infection is endemic in different animal species in southern Egypt. Screening herds for A. marginale is recommended even when the signs of anaplasmosis are absent.
Assessment of cypermethrin and amitraz resistance and molecular profiling of voltage-gated sodium channel and octopamine tyramine genes of Rhipicephalus microplus. International-journal Peer-reviewed

Muhammad Kashif Obaid, Mashal M Almutairi, Abdulaziz Alouffi, Sher Zaman Safi, Tetsuya Tanaka, Abid Ali

Frontiers in cellular and infection microbiology　13　1176013-1176013　2023

DOI： 10.3389/fcimb.2023.1176013 　

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Control of ticks and tick-borne pathogens is a priority for human and animal health. Livestock-holders extensively rely on acaricide applications for tick control. Different groups of acaricides including cypermethrin and amitraz have been consistently used in Pakistan. There has been a gap in understanding the susceptibility or resistance of Rhipicephalus microplus, the most prevalent tick in Pakistan, to acaricides. The present study aimed to molecularly characterize cypermethrin and amitraz targeted genes such as voltage-gated sodium channel (VGSC) and octopamine tyramine (OCT/Tyr) of R. microplus ticks in Khyber Pakhtunkhwa (KP), Pakistan to monitor the acaricides resistance. Tick specimens were collected from cattle and buffaloes in northern (Chitral, Shangla, Swat, Dir, and Buner), central (Peshawar, Mardan, Charsadda, Swabi, and Nowshera), and southern districts (Kohat, Karak, Lakki Marwat, Tank, and Dera Ismail Khan) of KP, Pakistan. Different concentrations of commercially available cypermethrin (10%) and amitraz (12.5%) were prepared for in vitro larval immersion tests (LIT). In LIT, the average mortality rate of immersed larvae was recorded that was increased gradually with an increase in the concentration of specific acaricide. The larvae's highest mortality rates (94.5% and 79.5%) were observed at 100-ppm of cypermethrin and amitraz, respectively. A subset of 82 R. microplus ticks was subjected to extract genomic DNA, followed by PCR to amplify partial fragments of VGSC (domain-II) and OCT/Tyr genes. The BLAST results of the consensus sequence of VGSC gene (domain-II) showed 100% identity with the acaricides susceptible tick sequence from the United States (reference sequence). Obtained identical sequences of OCT/Tyr genes showed maximum identity (94-100%) with the identical sequences reported from Australia (reference sequence), India, Brazil, Philippines, USA, South Africa, and China. Thirteen single nucleotide polymorphisms (10 synonymous and three non-synonymous) were observed at various positions of partial OCT/Tyr gene fragments. The SNP at position A-22-C (T-8-P) in OCT/Tyr gene has been linked to amitraz resistance in R. microplus ticks. Molecular analysis and LIT bioassay's findings indicate the availability of resistant R. microplus ticks in the KP region. To our understanding, this is the first preliminary study to monitor cypermethrin and amitraz resistance via molecular profiling of cypermethrin and amitraz targeted genes (VGSC and OCT/Tyr) in combination with in vitro bioassays (LIT) in R. microplus ticks from Pakistan.
Clinical and Pathological Findings in Fatal Cases of Severe Fever With Thrombocytopenia Syndrome With High Viremia in Cats. International-journal Peer-reviewed

Aya Matsuu, Hitoshi Hatai, Tatsuro Hifumi, Emu Hamakubo, Maho Take, Tetsuya Tanaka, Yasuyuki Momoi, Yasuyuki Endo, Ai Koyoshi, Yuka Kamikubo, Raye Kamatsuki, Natsuki Kurusu, Haruna Tabata, Hiroshi Matsuyama, Mei Yanaidani, Michiyo Kawabata, Takahiro Kawabata

Topics in companion animal medicine　52　100756-100756　2023

DOI： 10.1016/j.tcam.2022.100756 　

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Severe fever with thrombocytopenia syndrome (SFTS) is an emerging zoonotic disease caused by the SFTS virus (SFTSV). SFTSV causes severe symptoms both in humans and cats. In this study, we report the clinical and pathological findings of 4 fatal cases of cats with high SFTS viremia levels. These cats showed an acute onset of fever, leukopenia, thrombocytopenia, and increased serum amyloid A and pro-inflammatory cytokine levels. A high viral copy number was detected in the blood, oral swabs, rectal swabs, conjunctiva swabs, and urine. Histopathologically, necrotizing lymphadenitis, splenitis with lymphoblastoid cell proliferation, and hemophagocytosis were observed in all 4 cats. Immunohistochemistry revealed the presence of SFTSV antigen on lymphoblastoid B cells. SFTSV-RNA was detected in systemic tissues, including the brain. The present findings provide useful information for understanding the features of fatal SFTS in cats. To elucidate the mechanisms of severe progress of SFTS cats, as well as its role as a source of human infection, further research is needed.
Editorial: Methods and applications in invertebrate physiology. International-journal Peer-reviewed

Natraj Krishnan, Tetsuya Tanaka, Graziano Fiorito, Fernando Ariel Genta, Pamela Imperadore

Frontiers in physiology　14　1158972-1158972　2023

DOI： 10.3389/fphys.2023.1158972 　
A novel eukaryotic RdRP-dependent small RNA pathway represses antiviral immunity by controlling an ERK pathway component in the black-legged tick. International-journal Peer-reviewed

Canran Feng, Kyosuke Torimaru, Mandy Yu Theng Lim, Li-Ling Chak, Masami Shiimori, Kosuke Tsuji, Tetsuya Tanaka, Junko Iida, Katsutomo Okamura

PloS one　18　(3)　e0281195　2023

DOI： 10.1371/journal.pone.0281195 　

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Small regulatory RNAs (sRNAs) are involved in antiviral defense and gene regulation. Although roles of RNA-dependent RNA Polymerases (RdRPs) in sRNA biology are extensively studied in nematodes, plants and fungi, understanding of RdRP homologs in other animals is still lacking. Here, we study sRNAs in the ISE6 cell line, which is derived from the black-legged tick, an important vector of human and animal pathogens. We find abundant classes of ~22nt sRNAs that require specific combinations of RdRPs and sRNA effector proteins (Argonautes or AGOs). RdRP1-dependent sRNAs possess 5'-monophosphates and are mainly derived from RNA polymerase III-transcribed genes and repetitive elements. Knockdown of some RdRP homologs misregulates genes including RNAi-related genes and the regulator of immune response Dsor1. Sensor assays demonstrate that Dsor1 is downregulated by RdRP1 through the 3'UTR that contains a target site of RdRP1-dependent repeat-derived sRNAs. Consistent with viral gene repression by the RNAi mechanism using virus-derived small interfering RNAs, viral transcripts are upregulated by AGO knockdown. On the other hand, RdRP1 knockdown unexpectedly results in downregulation of viral transcripts. This effect is dependent on Dsor1, suggesting that antiviral immunity is enhanced by RdRP1 knockdown through Dsor1 upregulation. We propose that tick sRNA pathways control multiple aspects of immune response via RNAi and regulation of signaling pathways.
Description of Male, Redescription of Female, Host Record, and Phylogenetic Position of Haemaphysalis danieli. International-journal Peer-reviewed

Iftikhar Ahmad, Shafi Ullah, Abdulaziz Alouffi, Mashal M Almutairi, Mehran Khan, Muhammad Numan, Sher Zaman Safi, Lidia Chitimia-Dobler, Tetsuya Tanaka, Abid Ali

Pathogens (Basel, Switzerland)　11　(12)　2022/12/08

DOI： 10.3390/pathogens11121495 　

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Haemaphysalis ticks are globally distributed with the greatest diversity in the Oriental region. This study aimed to primarily provide information on the morphology, host record, and preliminary phylogenetic position of a poorly known tick Haemaphysalis danieli. Herds comprised of goats and sheep were examined for this tick species in Upper Dir, Khyber Pakhtunkhwa, Pakistan. A total of 127 ticks, including males (n = 15, 11.8%) and females (n = 112, 88.2%), were collected, and morphologically identified as H. danieli. The morphological identification was confirmed through the 16S rDNA and cytochrome c oxidase (cox1) sequences. Phylogenetic analysis inferred based on 16S rDNA and cox1 showed a close evolutionary relationship of H. danieli with a conspecific from China and an undetermined Haemaphysalis sp. from China and Anatolia. A total of 32/223 (14.3%) goats in two different herds were the only host infested by H. danieli. The earliest study provided the morphological description of H. danieli male, host record, and phylogenetic position. The information provided herein could assist in minimizing the knowledge gap regarding the systematic and taxonomy of Haemaphysalis species.
Low Genetic Polymorphism in the Immunogenic Sequences of Rhipicephalus microplus Clade C. International-journal Peer-reviewed

Ismail Zeb, Mashal M Almutairi, Abdulaziz Alouffi, Nabila Islam, Luís Fernando Parizi, Sher Zaman Safi, Tetsuya Tanaka, Itabajara da Silva Vaz Jr, Abid Ali

Vaccines　10　(11)　2022/11/11

DOI： 10.3390/vaccines10111909 　

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Rhipicephalus microplus tick highly affects the veterinary sector throughout the world. Different tick control methods have been adopted, and the identification of tick-derived highly immunogenic sequences for the development of an anti-tick vaccine has emerged as a successful alternate. This study aimed to characterize immunogenic sequences from R. microplus ticks prevalent in Pakistan. Ticks collected in the field were morphologically identified and subjected to DNA and RNA extraction. Ticks were molecularly identified based on the partial mitochondrial cytochrome C oxidase subunit (cox) sequence and screened for piroplasms (Theileria/Babesia spp.), Rickettsia spp., and Anaplasma spp. PCR-based pathogens-free R. microplus-derived cDNA was used for the amplification of full-length cysteine protease inhibitor (cystatin 2b), cathepsin L-like cysteine proteinase (cathepsin-L), glutathione S-transferase (GST), ferritin 1, 60S acidic ribosomal protein (P0), aquaporin 2, ATAQ, and R. microplus 05 antigen (Rm05Uy) coding sequences. The cox sequence revealed 100% identity with the nucleotide sequences of Pakistan's formerly reported R. microplus, and full-length immunogenic sequences revealed maximum identities to the most similar sequences reported from India, China, Cuba, USA, Brazil, Egypt, Mexico, Israel, and Uruguay. Low nonsynonymous polymorphisms were observed in ATAQ (1.5%), cathepsin-L (0.6%), and aquaporin 2 (0.4%) sequences compared to the homologous sequences from Mexico, India, and the USA, respectively. Based on the cox sequence, R. microplus was phylogenetically assembled in clade C, which includes R. microplus from Pakistan, Myanmar, Malaysia, Thailand, Bangladesh, and India. In the phylogenetic trees, the cystatin 2b, cathepsin-L, ferritin 1, and aquaporin 2 sequences were clustered with the most similar available sequences of R. microplus, P0 with R. microplus, R. sanguineus and R. haemaphysaloides, and GST, ATAQ, and Rm05Uy with R. microplus and R. annulatus. This is the first report on the molecular characterization of clade C R. microplus-derived immunogenic sequences.
Spatio-Temporal Patterns of Ticks and Molecular Survey of Anaplasma marginale, with Notes on Their Phylogeny. International-journal Peer-reviewed

Shumaila Alam, Mehran Khan, Abdulaziz Alouffi, Mashal M Almutairi, Shafi Ullah, Muhammad Numan, Nabila Islam, Zaibullah Khan, Ome Aiman, Sher Zaman Safi, Tetsuya Tanaka, Abid Ali

Microorganisms　10　(8)　2022/08/17

DOI： 10.3390/microorganisms10081663 　

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Hard ticks (Ixodida: Ixodidae) are medically important ectoparasites that feed on all classes of terrestrial vertebrates. Recently, we molecularly characterized hard ticks and associated Anaplasma spp. in the northern and central regions of Khyber Pakhtunkhwa (KP), Pakistan; however, this knowledge was missing in the southern regions. This study aimed to investigate tick prevalence, host range, genetic diversity, and molecular survey of Anaplasma spp. in a wide range of tick species in two distinct physiographic regions of southern KP. A total of 1873 hard ticks were randomly collected from 443/837 hosts (cattle, Asian water buffaloes, horses, goats, sheep, dogs, and camels) in Lakki Marwat, Bannu, and Orakzai districts of KP. Overall, 12 tick species were morphologically identified, among which Hyalomma dromedarii was the most prevalent species (390/1873, 20.9%), followed by Hy. anatolicum (294, 15.7%), Rhipicephalus microplus (262, 14%), Hy. scupense (207, 11.1%), R. sanguineus (136, 7.3%), R. turanicus (121, 6.5%), Haemaphysalis cornupunctata (107, 5.7%), R. haemaphysaloides (110, 5.9%), Ha. montgomeryi (87, 4.6%), Hy. isaaci (58, 3.1%), Ha. bispinosa (54, 2.9%), and Ha. sulcata (47, 2.5%). The extracted DNA from a subset of each tick species was subjected to PCR to amplify cox1 or 16S rRNA sequences of ticks and 16S rRNA sequences of Anaplasma spp. The tick cox1 sequences showed 99-100% identities with the sequences of the same species, whereas 16S rRNA sequences of R. turanicus, Ha. montgomeryi and Ha. sulcata showed 97-100% identities with the corresponding species. The 16S rRNA sequence of Ha. cornupunctata showed 92% identity with the species from the same subgenus, such as Ha. punctata. The 16S rRNA sequence of Anaplasma spp. showed 100% identity with Anaplasma marginale. Moreover, 54 ticks were found positive for A. marginale with a total infection rate of 17.2%. The highest infection rate was recorded in Hy. dromedarii (31.1%) and the lowest in each R. haemaphysaloides and R. sanguineus (20%). All the cox1 or 16S rRNA sequences in phylogenetic trees clustered with the same species, except Ha. cornupunctata, which clustered with the Ha. (Aboimisalis) punctata. In this study, Ha. cornupunctata was reported for the first time at the molecular level. The genetic characterization of ixodid ticks and molecular detection of associated A. marginale will assist in the epidemiological surveillance of these parasites in the region.
Molecular Characterization and Assessment of Risk Factors Associated with Theileria annulata Infection. International-journal Peer-reviewed

Karim Ullah, Muhammad Numan, Abdulaziz Alouffi, Mashal M Almutairi, Hafsa Zahid, Majid Khan, Zia Ul Islam, Atif Kamil, Sher Zaman Safi, Haroon Ahmed, Tetsuya Tanaka, Abid Ali

Microorganisms　10　(8)　2022/08/09

DOI： 10.3390/microorganisms10081614 　

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Theileria annulata is a tick-associated parasite that causes tropical theileriosis in livestock and is responsible for huge economic losses. Studies have been neglected on the effect of Theileria spp. on cattle in Khyber Pakhtunkhwa (KP), Pakistan. The present study was designed to determine the genetic diversity and assess the risk factors associated with Theileria infection in selected districts of KP. Information on the risk factors related to the Theileria infection was collected through a questionnaire. Blood samples were collected from symptomatic cattle from January 2019 to February 2020, identified morphologically through microscopic examination, and processed for molecular characterization using the 18S rRNA gene as a genetic marker. Of the 555 cattle examined (136, 24.5%) and (294, 53%) were found positive for Theileria spp. by microscopic examination and a PCR test, respectively. Based on the PCR test, the highest prevalence of infection was found in district Upper Dir (46/75, 61.3%), followed by Lower Dir (54/90, 60%); Malakand (51/88, 57.9%); Peshawar (40/80, 50%); and Charsadda (52/112, 46.4%), with the lowest in Bajaur (51/110, 46.34%). A BLAST analysis of the 18S rDNA sequences showed 99.5% identity with T. annulata. In a phylogenetic tree, the 18S rDNA sequence of T. annulata clustered with sequences from Pakistan, China, and Italy. A significant association was observed between the prevalence of infection and different host characteristics. The highest infection was found in adult cattle (216/360, 60%); females (218/377, 57.8%); and Holstein Friesian (120/180, 66.6%). Theileria infection was significantly associated with management practices. Higher infection rates were observed in free-grazing cattle (190/412, 42.2%); those kept in unhygienic conditions (246/405, 60.7%); cattle in combined farming systems (165/255, 64.8%); and those in congested stall systems (150/218, 68.8%). Seasonal patterns were found to be significantly associated with infection, and a higher infection rate was observed in summer (215/350, 61.4%) than in winter (79/205, 38.5%). Identified risk factors should be considered in designing practical control approaches to reduce the burden of Theileria infection. Large scale studies are required to explore the diversity of Theileria species in KP, Pakistan.
Morphological identification and molecular characterization of economically important ticks (Acari: Ixodidae) from North and North-Western Egypt. International-journal Peer-reviewed

Haytham Senbill, Tetsuya Tanaka, Donia Karawia, Sahidur Rahman, Jehan Zeb, Olivier Sparagano, Aiswarya Baruah

Acta tropica　231　106438-106438　2022/07

DOI： 10.1016/j.actatropica.2022.106438 　

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Ticks represent a major source of growing economic and public health concern, especially in the tropics and sub-tropics. Towards evidencing ticks' invasion in the North and North-Western parts of Egypt, the present study aimed to investigate the morpho-molecular aspects of those ectoparasites using stereomicroscopy, scanning electron microscopy (SEM) and sequencing of the mitochondrial 16S ribosomal DNA gene (rDNA) and nuclear second internal transcribed spacer (ITS2). Our analysis confirmed the presence and well-distribution of Rhipicephalus sanguineus s.l. infesting dogs and sheep (Alexandria governorate), Rhipicephalus annulatus infesting cattle (Beheira governorate), and Hyalomma dromedarii infesting camels (Marsa Matruh governorate) from North/North-Western Egypt. 16S rDNA and ITS2 sequences of the ticks were amplified using universal and gene-specific sets of primers, sequenced and analyzed. Lengths of amplified 16S rDNA sequences in all examined tick species were found to be similar in size (approximately 460 bp); however, they differed in base pair constitutions, whereas ITS2 lengths were 1,500 bp, 1,550 bp, and 1,800 bp for Rh. annulatus, Rh. sanguineus s.l., and Hy. dromedarii, respectively. Phylogenetically, based on the 16S rDNA results, Rh. sanguineus s.l. ticks clustered with the southeastern Europe lineage from Romania and Greece, Rh. annulatus ticks were similar to Turkish populations, and Hy. dromedarii were close to the isolates from Tunisia. Similarly, based on ITS2 sequences, Rh. sanguineus s.l. from dogs were showing 99% similarity to Nigerian populations; however, those collected from sheep were closer to Iranian populations with 4.1% nucleotide divergence between the two populations of different hosts. Rh. annulatus ticks were identical to a population from Romania, whereas Hy. dromedarii was close by 99.7% similarity to a population from Kenya. This is the first study reporting nucleotide sequences of 16S rDNA and ITS2 in integration with morphological identification of ticks from this part of Egypt.
Molecular Survey and Genetic Characterization of Anaplasma marginale in Ticks Collected from Livestock Hosts in Pakistan. International-journal Peer-reviewed

Zaibullah Khan, Shehla Shehla, Abdulaziz Alouffi, Muhammad Kashif Obaid, Alam Zeb Khan, Mashal M Almutairi, Muhammad Numan, Ome Aiman, Shumaila Alam, Shafi Ullah, Sher Zaman Safi, Tetsuya Tanaka, Abid Ali

Animals : an open access journal from MDPI　12　(13)　2022/07/01

DOI： 10.3390/ani12131708 　

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Ticks transmit pathogens to animals and humans more often than any other arthropod vector. The rural economy of Pakistan mainly depends on livestock farming, and tick infestations cause severe problems in this sector. The present study aimed to molecularly characterize the Anaplasma spp. in hard ticks collected from six districts of Khyber Pakhtunkhwa, Pakistan. Ticks were collected from various livestock hosts, including cattle breeds (Holstein-Friesian, Jersey, Sahiwal, and Achai), Asian water buffaloes, sheep, and goats from March 2018 to February 2019. Collected ticks were morphologically identified and subjected to molecular screening of Anaplasma spp. by amplifying 16S rDNA sequences. Six hundred seventy-six ticks were collected from infested hosts (224/350, 64%). Among the nine morphologically identified tick species, the highest occurrence was noted for Rhipicephalus microplus (254, 37.6%), followed by Hyalomma anatolicum (136, 20.1%), Rhipicephalus haemaphysaloides (119, 17.6%), Rhipicephalus turanicus (116, 17.1%), Haemaphysalis montgomeryi (14, 2.1%), Hyalomma dromedarii (11, 1.6%), Haemaphysalis bispinosa (10, 1.5%), Hyalomma scupense (8, 1.2%), and Haemaphysalis kashmirensis (8, 1.2%). The occurrence of tick females was highest (260, 38.5%), followed by nymphs (246, 36.4%) and males (170, 25.1%). Overall, the highest occurrence of ticks was recorded in the Peshawar district (239, 35.3%), followed by Mardan (183, 27.1%), Charsadda (110, 16.3%), Swat (52, 7.7%), Shangla (48, 7.1%), and Chitral (44, 6.5%). Among these ticks, Anaplasma marginale was detected in R. microplus, R. turanicus, and R. haemaphysaloides. The 16S rDNA sequences showed high identity (98-100%) with A. marginale reported from Australia, China, Japan, Pakistan, Thailand, Uganda, and the USA. In phylogenetic analysis, the sequence of A. marginale clustered with the same species reported from Australia, China, Pakistan, Thailand, Uruguay, and the USA. Further molecular work regarding the diversity of tick species and associated pathogens is essential across the country.
Ambivalent Roles of Oxidative Stress in Triangular Relationships among Arthropod Vectors, Pathogens and Hosts. International-journal Peer-reviewed

Emmanuel Pacia Hernandez, Anisuzzaman, Md Abdul Alim, Hayato Kawada, Kofi Dadzie Kwofie, Danielle Ladzekpo, Yuki Koike, Takahiro Inoue, Sana Sasaki, Fusako Mikami, Makoto Matsubayashi, Tetsuya Tanaka, Naotoshi Tsuji, Takeshi Hatta

Antioxidants (Basel, Switzerland)　11　(7)　2022/06/25

DOI： 10.3390/antiox11071254 　

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Blood-feeding arthropods, particularly ticks and mosquitoes are considered the most important vectors of arthropod-borne diseases affecting humans and animals. While feeding on blood meals, arthropods are exposed to high levels of reactive oxygen species (ROS) since heme and other blood components can induce oxidative stress. Different ROS have important roles in interactions among the pathogens, vectors, and hosts. ROS influence various metabolic processes of the arthropods and some have detrimental effects. In this review, we investigate the various roles of ROS in these arthropods, including their innate immunity and the homeostasis of their microbiomes, that is, how ROS are utilized to maintain the balance between the natural microbiota and potential pathogens. We elucidate the mechanism of how ROS are utilized to fight off invading pathogens and how the arthropod-borne pathogens use the arthropods' antioxidant mechanism to defend against these ROS attacks and their possible impact on their vector potentials or their ability to acquire and transmit pathogens. In addition, we describe the possible roles of ROS in chemical insecticide/acaricide activity and/or in the development of resistance. Overall, this underscores the importance of the antioxidant system as a potential target for the control of arthropod and arthropod-borne pathogens.
Molecular Detection and Characterization of Babesia and Theileria in Cattle and Water Buffaloes from Southern Luzon, Philippines. International-journal Peer-reviewed

Ian Cary B Prado, Larry Xerxes B Capuno Jr, Princess Dlp Collera, Aaron Paul D Cabralda, Kristina Andrea S De Ramos, John Michael G Bernardo, Billy P Divina, Tatsunori Masatani, Tetsuya Tanaka, Remil L Galay

Microorganisms　10　(4)　2022/03/22

DOI： 10.3390/microorganisms10040678 　

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Babesia and Theileria are tick-borne protozoan parasites that can cause significant economic losses in the cattle industry. This study aimed to contribute to the limited epidemiological data on Theileria orientalis as well as Babesia bigemina and B. bovis in large ruminants in the Philippines. Blood samples of 412 cattle and 108 water buffalo collected from four provinces in Southern Luzon, Philippines, were initially tested for the 18S rRNA gene of piroplasms through nested PCR. Positive samples were further subjected to species-specific PCR. The 18s rRNA of piroplasms was detected in 123 (29.9%) cattle and three (2.8%) water buffaloes. Theileria orientalis was found to be the most common piroplasm in cattle with a detection rate of 17.5%, followed by Babesia bovis and B. bigemina. Co-infections were also observed. Two water buffaloes were found infected with B. bovis, while one was positive for B. bigemina. The phylogenetic tree for B. bovis showed clustering of the isolates in two clades together with isolates from other countries, and a third separate clade. Meanwhile, the T. orientalis isolates in this study were distributed in three clades together with reported isolates from other countries. This study confirms the presence of T. orientalis in the Philippines and reports the genetic diversity of B. bovis and T. orientalis.
Rapid Spread of Severe Fever with Thrombocytopenia Syndrome Virus by Parthenogenetic Asian Longhorned Ticks. International-journal International-coauthorship Peer-reviewed

Xing Zhang, Chaoyue Zhao, Chaoyuan Cheng, Guogang Zhang, Tao Yu, Kevin Lawrence, Hongyue Li, Jimin Sun, Zeyu Yang, Ling Ye, Hongliang Chu, Ying Wang, Xiaohu Han, Yongchao Jia, Shuozhang Fan, Hirotaka Kanuka, Tetsuya Tanaka, Cheryl Jenkins, Kristene Gedye, Shona Chandra, Dana C Price, Qiyong Liu, Young Ki Choi, Xiangjiang Zhan, Zhibin Zhang, Aihua Zheng

Emerging infectious diseases　28　(2)　363-372　2022/02

DOI： 10.3201/eid2802.211532 　

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Severe fever with thrombocytopenia syndrome virus (SFTSV) is spreading rapidly in Asia. This virus is transmitted by the Asian longhorned tick (Haemaphysalis longicornis), which has parthenogenetically and sexually reproducing populations. Parthenogenetic populations were found in ≥15 provinces in China and strongly correlated with the distribution of severe fever with thrombocytopenia syndrome cases. However, distribution of these cases was poorly correlated with the distribution of populations of bisexual ticks. Phylogeographic analysis suggested that the parthenogenetic population spread much faster than bisexual population because colonization is independent of sexual reproduction. A higher proportion of parthenogenetic ticks was collected from migratory birds captured at an SFTSV-endemic area, implicating the contribution to the long-range movement of these ticks in China. The SFTSV susceptibility of parthenogenetic females was similar to that of bisexual females under laboratory conditions. These results suggest that parthenogenetic Asian longhorned ticks, probably transported by migratory birds, play a major role in the rapid spread of SFTSV.
Molecular Survey and Spatial Distribution of Rickettsia spp. in Ticks Infesting Free-Ranging Wild Animals in Pakistan (2017-2021). International-journal Peer-reviewed

Abid Ali, Shehla Shehla, Hafsa Zahid, Farman Ullah, Ismail Zeb, Haroon Ahmed, Itabajara da Silva Vaz Jr, Tetsuya Tanaka

Pathogens (Basel, Switzerland)　11　(2)　2022/01/26

DOI： 10.3390/pathogens11020162 　

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Rickettsia spp. associated with ticks infesting wild animals have been mostly neglected in several countries, including Pakistan. To address this knowledge gap, ticks were collected during 2017 to 2021 from wild animals including cats (Felis chaus), Indian hedgehogs (Paraechinus micropus), and wild boars (Sus scrofa). The collected ticks were morpho-molecularly identified and screened for the detection of Rickettsia spp. Morphologically identified ticks were categorized into four species of the genus Rhipicephalus: Rhipicephalus haemaphysaloides, Rh. turanicus, Rh. sanguineus sensu lato (s.l), and Rh. microplus. Among 53 wild animals examined, 31 were infested by 531 ticks, an overall prevalence of 58.4%. Adult female ticks were predominant (242 out of 513 ticks collected, corresponding to 46%) in comparison with males (172, 32%), nymphs (80, 15%) and larvae (37, 7%). The most prevalent tick species was Rh. turanicus (266, 50%), followed by Rh. microplus (123, 23%), Rh. sanguineus (106, 20%), and Rh. haemaphysaloides (36, 7%). Among the screened wild animals, wild boars were the most highly infested, with 268 ticks being collected from these animals (50.4%), followed by cats (145, 27.3%) and hedgehogs (118, 22.3%). Tick species Rh. haemaphysaloides, Rh. turanicus, and Rh. sanguineus were found on wild boars, Rh. haemaphysaloides, and Rh. microplus on cats, and Rh. turanicus on hedgehogs. In a phylogenetic analysis, mitochondrial cytochrome C oxidase 1 (cox1) sequences obtained from a subsample (120) of the collected ticks clustered with sequences from Bangladesh, China, India, Iran, Myanmar, and Pakistan, while 16S ribosomal DNA (16S rDNA) sequences clustered with sequences reported from Afghanistan, Egypt, India, Pakistan, Romania, Serbia, and Taiwan. Among Rickettsia infected ticks (10/120, 8.3%), Rh. turanicus (7/10, 70%), and Rh. haemaphysaloides (3/10, 30%) were found infesting wild boars in the districts Mardan and Charsadda. The obtained rickettsial gltA gene sequences showed 99% and ompA gene sequences showed 100% identity with Rickettsia massiliae, and the phylogenetic tree shows ompA clustered with the same species reported from France, Greece, Spain, and USA. This study emphasizes the need for effective surveillance and control programs in the region to prevent health risks due to tick-borne pathogens, and that healthy infested wild animals may play a role in the spread of these parasites.
Host Immune Responses to Salivary Components - A Critical Facet of Tick-Host Interactions. International-journal Peer-reviewed

Abid Ali, Ismail Zeb, Abdulaziz Alouffi, Hafsa Zahid, Mashal M Almutairi, Fahdah Ayed Alshammari, Mohammed Alrouji, Carlos Termignoni, Itabajara da Silva Vaz Jr, Tetsuya Tanaka

Frontiers in cellular and infection microbiology　12　809052-809052　2022

DOI： 10.3389/fcimb.2022.809052 　

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Tick sialome is comprised of a rich cocktail of bioactive molecules that function as a tool to disarm host immunity, assist blood-feeding, and play a vibrant role in pathogen transmission. The adaptation of the tick's blood-feeding behavior has lead to the evolution of bioactive molecules in its saliva to assist them to overwhelm hosts' defense mechanisms. During a blood meal, a tick secretes different salivary molecules including vasodilators, platelet aggregation inhibitors, anticoagulants, anti-inflammatory proteins, and inhibitors of complement activation; the salivary repertoire changes to meet various needs such as tick attachment, feeding, and modulation or impairment of the local dynamic and vigorous host responses. For instance, the tick's salivary immunomodulatory and cement proteins facilitate the tick's attachment to the host to enhance prolonged blood-feeding and to modulate the host's innate and adaptive immune responses. Recent advances implemented in the field of "omics" have substantially assisted our understanding of host immune modulation and immune inhibition against the molecular dynamics of tick salivary molecules in a crosstalk between the tick-host interface. A deep understanding of the tick salivary molecules, their substantial roles in multifactorial immunological cascades, variations in secretion, and host immune responses against these molecules is necessary to control these parasites. In this article, we reviewed updated knowledge about the molecular mechanisms underlying host responses to diverse elements in tick saliva throughout tick invasion, as well as host defense strategies. In conclusion, understanding the mechanisms involved in the complex interactions between the tick salivary components and host responses is essential to decipher the host defense mechanisms against the tick evasion strategies at tick-host interface which is promising in the development of effective anti-tick vaccines and drug therapeutics.
Acaricides Resistance in Ticks: Selection, Diagnosis, Mechanisms, and Mitigation. International-journal Peer-reviewed

Muhammad Kashif Obaid, Nabila Islam, Abdulaziz Alouffi, Alam Zeb Khan, Itabajara da Silva Vaz Jr, Tetsuya Tanaka, Abid Ali

Frontiers in cellular and infection microbiology　12　941831-941831　2022

DOI： 10.3389/fcimb.2022.941831 　

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Ticks are blood-feeding ecto-parasites that have a cosmopolitan distribution in tropical and subtropical regions of the world. Ticks cause economic losses in the form of reduced blood, meat and dairy products, as well as pathogen transmission. Different acaricides such as organochlorines, organophosphates, formamidines (e.g. amitraz), synthetic pyrethroids, macrocyclic lactones, fipronil, and fluazuron are currently used sequentially or simultaneously to control tick infestations. Most acaricide treatments now face increasingly high chances of failure, due to the resistance selection in different tick populations against these drugs. Acaricide resistance in ticks can be developed in different ways, including amino acid substitutions that result in morphological changes in the acaricide target, metabolic detoxification, and reduced acaricide entry through the outer layer of the tick body. The current literature brings a plethora of information regarding the use of different acaricides for tick control, resistance selection, analysis of mutations in target sites, and resistance mitigation. Alternatives such as synergistic use of different acaricides, plant-derived phytochemicals, fungi as biological control agents, and anti-tick vaccines have been recommended to avoid and mitigate acaricide resistance. The purpose of this review was to summarize and discuss different acaricides applied for tick control, their mechanisms of action and resistance selection, genetic polymorphisms in their target molecules, as well as the approaches used for diagnosis and mitigation of acaricide resistance, specifically in Rhipicephalus microplus ticks.
Host Immunization with Recombinant Tick Antigen and Evaluation of Host Immune Response. International-journal Peer-reviewed

Kodai Kusakisako, Takeshi Miyata, Kozo Fujisaki, Tetsuya Tanaka

Methods in molecular biology (Clifton, N.J.)　2411　331-341　2022

DOI： 10.1007/978-1-0716-1888-2_19 　

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Ticks are classified as hematophagous arthropods and transfer a variety of pathogens-such as viruses, bacteria, and protozoans-to vertebrate hosts during blood feeding. These transmitted pathogens cause infectious diseases that continue to affect both humans and animals worldwide. Chemical acaricides are commonly used for tick control to prevent infectious diseases. However, the continuous use of acaricides leads to the emergence of acaricide-resistant tick species; thus, alternative methods for tick control are necessary. Vaccination of vertebrate hosts with tick-derived molecules is considered to be a better alternative against ticks than chemical acaricides because ticks feed on host blood for several days and also concentrate the host blood with antibodies. On the other hand, the host's immune responses against pathogens mainly take two pathways-Th1 (cell-mediated immunity) and Th2 (humoral immunity) pathways. Thus, the vaccine can suggest which immune pathway is more important for vaccination. This chapter describes the procedures of immunizing laboratory animals-mice-with a recombinant tick protein for the preliminary evaluation of its potential as an anti-tick vaccine candidate. In addition, the method of evaluating the antigen-specific antibody production in the host using ELISA is described, as is the subsequent tick-infestation challenge for determining the effectiveness of vaccination.
Fundamental Tick Vaccinomic Approach to Evade Host Autoimmune Reaction. International-journal Peer-reviewed

Seham H M Hendawy, Heba F Alzan, Tetsuya Tanaka, Mona S Mahmoud

Methods in molecular biology (Clifton, N.J.)　2411　343-358　2022

DOI： 10.1007/978-1-0716-1888-2_20 　

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Ticks are obligate hematophagous ectoparasites that infect domestic animals, humans, and wildlife. Ticks can transmit a wide range of pathogens (viruses, rickettsia, bacteria, parasites, etc.), and some of those are of zoonotic importance. Tick-borne diseases have a negative economic impact in several tropical and subtropical countries. With climate change, tick distribution and tick-associated pathogens have increased. Currently, tick control procedures have more environmental drawbacks and there are pitfalls in vaccination process. Since vaccinations have helped to prevent several diseases and infections, several vaccination trials are ongoing to control ticks and tick-borne pathogens. However, autoimmune reactions to vaccinations are reported as an adverse reaction since vaccines were used to protect against disease in humans and animals. The antibodies against the vaccine antigen might harm similar antigen in the host. Therefore, in this chapter, we attempt to shed light on the importance of raising awareness of possible adverse events associated with vaccinations and the methods that should be used to address this problem. In silico and lab work should be performed ahead of the vaccination process to evaluate the vaccine candidates and avoid the vaccination opposing consequences.
Development of a loop-mediated isothermal amplification (LAMP) assay targeting the mitochondrial cytochrome b gene for the rapid detection of alveolar echinococcosis in hepatic nodules of horses. International-journal Peer-reviewed

Tatsuro Hifumi, Kohei Akioka, Tetsuya Tanaka, Noriaki Miyoshi

Veterinary parasitology　299　109573-109573　2021/09/11

DOI： 10.1016/j.vetpar.2021.109573 　

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Alveolar echinococcosis, which is caused by a larval-stage infection of Echinococcus multilocularis, is a zoonosis with public health importance. Recently, alveolar echinococcosis in slaughtered horses has been reported in Japan and Poland. In terms of public health, a highly sensitive and specific diagnostic method is essential for early detection during meat inspection. In this study, the loop-mediated isothermal amplification (LAMP) assay was developed and validated to target the mitochondrial cytochrome b (cob) gene of E. multilocularis. Forty-one hepatic solid nodules obtained from each horse were evaluated based on histopathological examination and cob-targeted PCR and then submitted to the LAMP assay. The optimal condition of the developed LAMP assay was 64℃ for 30 min. The results of the developed LAMP assay were completely consistent with those of cob PCR. In addition, the detection limit for the number of copies of the cob gene was 135 copies/μL in the LAMP assay. These findings suggest that the ability of the LAMP assay developed in this study is equivalent to that of the conventional PCR testing. The LAMP assay developed in this study can be used as an alternative to PCR testing for the routine genetic diagnosis of alveolar echinococcosis in horses.
Chromosome-Level Genome Sequence of Aspergillus puulaauensis MK2, a Fungus Isolated from a Dead Hard Tick. International-journal Peer-reviewed

Taiki Futagami, Kazuki Mori, Chihiro Kadooka, Hiroko Niihara, Kosuke Tashiro, Hisanori Tamaki, Tetsuya Tanaka

Microbiology resource announcements　10　(36)　e0037221　2021/09/09

DOI： 10.1128/MRA.00372-21 　

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Aspergillus puulaauensis strain MK2 was isolated from a dead hard tick (Haemaphysalis longicornis). Here, we determined the chromosome-level genome sequence of A. puulaauensis MK2.
Promoter analysis of elongation factor I-alpha(EFI α) derived from Haemaphysalis longicornis(和訳中)

Hernandez Emmanuel Pacia, 川田逸人, 山本大介, 加藤大智, 松林誠, 田仲哲也, 辻尚利, 八田岳士

日本寄生虫学会・日本臨床寄生虫学会合同大会プログラム・抄録集　90回・32回　58-58　2021/04
Publisher: 日本寄生虫学会・日本臨床寄生虫学会
Relationship between hepatic grayish-white solid nodules in horses imported from Canada and larval Echinococcus multilocularis infection. International-journal Peer-reviewed

Tatsuro Hifumi, Tetsuya Tanaka, Emmanuel Pacia Hernandez, Kohei Akioka, Kaori Yamada, Yasutaka Imamura, Hitoshi Hatai, Noriaki Miyoshi

The Canadian veterinary journal = La revue veterinaire canadienne　62　(3)　285-288　2021/03

ISSN： 0008-5286

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Histopathological and genetic examinations were conducted on grayish-white solid hepatic nodules in 150 horses imported from Canada, in order to investigate larval Echinococcus multilocularis infection. Ten of the 150 horses (6.7%) were diagnosed with alveolar hydatid disease. The sequences of the mitochondrial cytochrome b genes obtained from all 10 polymerase chain reaction positive samples had 99 to 100% identity with the European haplotype E1 of E. multilocularis. Therefore, we concluded that the infections likely originated in Canada.
Determination of resistance status to amitraz in the cattle tick Rhipicephalus (Boophilus) microplus from Luzon, Philippines, through bioassay and molecular analysis. International-journal Peer-reviewed

Sherwin L Alota, Tisha Rogelle J Edquiban, Remil L Galay, John Michael G Bernardo, Kristina Andrea C Sandalo, Billy P Divina, Tetsuya Tanaka

Experimental & applied acarology　83　(3)　399-409　2021/02/16

DOI： 10.1007/s10493-021-00593-8 　

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Tick infestations and tick-borne diseases are among the leading causes of economic losses in the cattle industry worldwide. Amitraz is one of the most commonly used chemical acaricides against the cattle tick Rhipicephalus (Boophilus) microplus. Resistance to amitraz has been reported in many countries but not in the Philippines. This study aimed to determine whether cattle ticks from provinces in northern and southern Luzon, Philippines, are resistant to amitraz. Engorged or nearly engorged female ticks were collected from 21 farms and allowed to lay eggs to produce larvae. Larval packet test (LPT) was performed using three concentrations of amitraz, as well as a negative control (diluent). Reverse-transcription polymerase chain reaction (RT-PCR) was also performed to amplify a fragment of the octopamine/tyramine receptor gene and was subjected to sequence analysis by multiple nucleotide and amino acid sequence alignments together with reference strain, amitraz-susceptible strain, and amitraz-resistant strain reported in other countries. LPT results suggest the absence of resistance in all of the tested populations. Interestingly, analysis of the octopamine/tyramine receptor amino acid sequence revealed four out of 14 larval pools having substitutions similar to that of the reported amitraz-resistant strains. Although no apparent resistance was observed in this study, prudent use of amitraz should be practiced as the development of resistance is still likely to occur in the future.
The antiviral immunity of ticks against transmitted viral pathogens. International-journal Peer-reviewed

Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Takeshi Hatta, Kentaro Yoshii, Kodai Kusakisako, Naotoshi Tsuji, Tetsuya Tanaka

Developmental and comparative immunology　119　104012-104012　2021/01/21

DOI： 10.1016/j.dci.2021.104012 　

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Ticks, being obligate hematophagous arthropods, are exposed to various blood-borne pathogens, including arboviruses. Consequently, their feeding behavior can readily transmit economically important viral pathogens to humans and animals. With this tightly knit vector and pathogen interaction, the replication and transmission of tick-borne viruses (TBVs) must be highly regulated by their respective tick vectors to avoid any adverse effect on the ticks' biological development and viability. Knowledge about the tick-virus interface, although gaining relevant advances in recent years, is advancing at a slower pace than the scientific developments related to mosquito-virus interactions. The unique and complicated feeding behavior of ticks, compared to that of other blood-feeding arthropods, also limits the studies that would further elaborate the antiviral immunity of ticks against TBVs. Hence, knowledge of molecular and cellular immune mechanisms at the tick-virus interface, will further elucidate the successful viral replication of TBVs in ticks and their effective transmission to human and animal hosts.
Molecular Prevalence of Anaplasma marginale and Ehrlichia in Domestic Large Ruminants and Rhipicephalus (Boophilus) microplus Ticks From Southern Luzon, Philippines. International-journal Peer-reviewed

Remil L Galay, Carina R Llaneta, Maria Karla Faye B Monreal, Antero L Armero 3rd, Arianne Bel D Baluyut, Czarina Marie F Regino, Kristina Andrea C Sandalo, Billy P Divina, Melbourne R Talactac, Lennox P Tapawan, Maarten Czar L Mojares, Cherry R Alvarez, Emmanuel R Mago, Noemi D Encarnacion, Masako Andoh, Tetsuya Tanaka

Frontiers in veterinary science　8　746705-746705　2021

DOI： 10.3389/fvets.2021.746705 　

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Anaplasmosis and ehrlichiosis are tick-borne rickettsial diseases that cause significant economic losses in the livestock industry worldwide. Although bovine anaplasmosis is known to be endemic in the Philippines, epidemiological data is fragmented. Moreover, little is known about bovine ehrlichiosis in the country. In this study, the prevalence of Anaplasma marginale and Ehrlichia in cattle and water buffalo from provinces in the southern part of Luzon, Philippines, was investigated through PCR. Blood samples from 620 animals comprised of 512 cattle and 108 water buffalo and 195 tick samples were subjected to nested PCR targeting the groESL gene of Anaplasmataceae. Positive samples were further subjected to another nested PCR and conventional PCR to amplify the A. marginale groEL gene and the Ehrlichia dsbA gene, respectively. Selected A. marginale-positive samples were also subjected to nested PCR targeting the msp5 gene. Regardless of the animal host, the overall prevalence in blood samples obtained was 51.9% for Anaplasmataceae, 43% for A. marginale, and 1.1% for Ehrlichia. No water buffalo were positive for Ehrlichia. Meanwhile, 15.9, 6.7, and 2% of the tick samples, all morphologically identified as Rhipicephalus (Boophilus) microplus, were positive for Anaplasmataceae, A. marginale, and Ehrlichia, respectively. Sequence analysis of selected A. marginale msp5 amplicons showed that the isolates from the region share 94-98% identity to reported A. marginale from other countries. The phylogenetic tree showed clustering of isolates in the region and a close relationship with A. marginale isolates from other countries. Sequences of Ehrlichia amplicons from cattle and ticks were 97-100% similar to reported Ehrlichia minasensis isolates. This study showed the high prevalence of A. marginale in Luzon, Philippines, and provided the first molecular evidence of E. minasensis in the country.
An Ixodes scapularis glutathione S-transferase plays a role in cell survival and viability during Langat virus infection of a tick cell line. International-journal Peer-reviewed

Emmanuel Pacia Hernandez, Melbourne Rio Talactac, Rodel Jonathan Santos Vitor, Kentaro Yoshii, Tetsuya Tanaka

Acta tropica　214　105763-105763　2020/11/23

DOI： 10.1016/j.actatropica.2020.105763 　

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Ticks are important vectors of diseases affecting both humans and animals. To be an efficient vector, ticks have to survive infection by pathogens such as the Langat virus (LGTV). One method utilized by ticks is their complex antioxidant mechanism. Included in the vast antioxidant processes are several enzymes involved in redox homeostasis. The ubiquitous glutathione S-transferases (GSTs) belong to the antioxidant family of enzymes. In this study, we evaluated the role of a GST during LGTV infection. ISE6 cells were infected with LGTV with a multiplicity of infection (MOI) of 0.01 and observed daily. The infection success was monitored via indirect immunofluorescent antibody test (IFAT) for LGTV for up to 4 days. The gene expression of IsGST1 was determined by real-time polymerase chain reaction (PCR) using IsGST1 gene-specific primers. Knockdown of the IsGST1 gene with subsequent LGTV infection was also performed. Afterward, ISE6 cell mortality and viability were checked daily until the fourth day. The virus titer from supernatants of IsGST1-knockdown cells was quantified using a focus-formation assay. IFAT data showed that LGTV infects ISE6 cells in a time-dependent manner with increasing infection from day 0 to day 4. The IsGST1 genes showed an increasing expression until day 2 of infection, while decreased expression was observed from day 3 to day 4 post-infection. Knockdown of the IsGST1 resulted in increased mortality on the third day of infection, while the cell viability was also negatively affected by the knockdown of the IsGST1 genes from day 0 to day 4 post-infection. Knockdown of the IsGST1 genes also resulted in a decreased viral titer from the supernatants of the ISE6 cells infected with LGTV. Based on the results, GSTs are possibly utilized both by cells and the virus for mutual survival and proliferation.
Development of a Loop-Mediated Isothermal Amplification (LAMP) Assay Targeting the Citrate Synthase Gene for Detection of Ehrlichia canis in Dogs Peer-reviewed

Angela Patricia B. Chua, Remil L. Galay, Tetsuya Tanaka, Wataru Yamazaki

Veterinary Sciences　7　(4)　156-156　2020/10/15
Publisher: MDPI AG
DOI： 10.3390/vetsci7040156 　

eISSN： 2306-7381

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Canine monocytic ehrlichiosis caused by Ehrlichia canis is one of the leading tick-borne diseases of dogs, particularly in tropical countries. A highly sensitive and specific diagnostic method is essential for early detection to facilitate treatment. This study was conducted to develop E. canis loop-mediated isothermal amplification (LAMP) assay, a highly sensitive yet simple molecular technique, targeting the citrate synthase (gltA) gene of E. canis. Canine blood samples were subjected to conventional PCR targeting E. canis gltA. After analysis of the sequences of PCR amplicons, LAMP primers were generated. The optimum temperature and time for the LAMP assay were determined using eight samples—after which, the effectiveness and reproducibility of LAMP were verified by testing 40 samples, which included PCR-positive and negative samples. The detection limit was also established. The optimal condition for the assay was 61 °C for 60 min. Compared to PCR, the LAMP assay had a relative sensitivity and specificity of 92.5 and 100%, respectively. Statistical analysis using McNemar’s test showed that the E. canis LAMP assay has no significant difference with PCR. Therefore, the LAMP assay developed in this study may be used as an alternative to PCR in the detection of E. canis.
ダニ研究の最前線とダニ媒介性感染症制御の可能性を探るマダニの吸血生理と抗マダニワクチン Peer-reviewed

田仲哲也

衛生動物　71　(2)　57-64　2020/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
Molecular Detection of Rickettsia Spp. and Coxiella Burnetii in Cattle, Water Buffalo, and Rhipicephalus (Boophilus) Microplus Ticks in Luzon Island of the Philippines. International-journal Peer-reviewed

Remil L Galay, Melbourne R Talactac, Bea V Ambita-Salem, Dawn Maureen M Chu, Lali Marie O Dela Costa, Cinnamon Mae A Salangsang, Darwin Kyle B Caracas, Florante H Generoso, Jonathan A Babelonia, Joeneil L Vergano, Lena C Berana, Kristina Andrea C Sandalo, Billy P Divina, Cherry R Alvarez, Emmanuel R Mago, Masako Andoh, Tetsuya Tanaka

Tropical medicine and infectious disease　5　(2)　2020/04/04

DOI： 10.3390/tropicalmed5020054 　

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Rickettsia and Coxiella burnetii are zoonotic, tick-borne pathogens that can cause febrile illnesses with or without other symptoms in humans, but may cause subclinical infections in animals. There are only a few reports on the occurrence of these pathogens in cattle and water buffalo in Southeast Asia, including the Philippines. In this study, molecular detection of Rickettsia and C. burnetii in the blood and in the Rhipicephalus (Boophilus) microplus ticks of cattle and water buffalo from five provinces in Luzon Island of the Philippines was done. A total of 620 blood samples of cattle and water buffalo and 206 tick samples were collected and subjected to DNA extraction. After successful amplification of control genes, nested PCR was performed to detect gltA of Rickettsia and com1 of C. burnetii. No samples were positive for Rickettsia, while 10 (cattle = 7, water buffaloes = 3), or 1.6% of blood, and five, or 1.8% of tick samples, were C. burnetii-positive. Sequence analysis of the positive amplicons showed 99-100% similarity to reported C. burnetii isolates. This molecular evidence on the occurrence of C. burnetii in Philippine ruminants and cattle ticks and its zoonotic nature should prompt further investigation and surveillance to facilitate its effective control.
Expression analysis of glutathione S-transferases and ferritins during the embryogenesis of the tick Haemaphysalis longicornis. International-journal Peer-reviewed

Emmanuel Pacia Hernandez, Kei Shimazaki, Hiroko Niihara, Rika Umemiya-Shirafuji, Kozo Fujisaki, Tetsuya Tanaka

Heliyon　6　(3)　e03644　2020/03

DOI： 10.1016/j.heliyon.2020.e03644 　

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In the tick life cycle, embryogenesis is the only stage of development wherein no blood meal is required. Nevertheless, even in the absence of a blood meal, which is the source of nutrients as well as the ferrous iron and heme that could cause oxidative stress in ticks, malondialdehyde (MDA) has been reported to increase during this period. Additionally, the knockdown of some oxidative stress-related molecules such as ferritin has resulted in abnormal eggs and embryonic death. Here, we investigate the gene and protein expression profiles of the identified glutathione S-transferases (GSTs) and ferritins (Fers) of the tick H. longicornis during embryogenesis through quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blotting, respectively. We also confirm the lipid peroxidation and ferrous iron concentration level using a thiobarbituric acid reactive substances (TBARS) assay. Finally, we attempt to correlate these findings with the events occurring by establishing a staging process in H. longicornis embryos. Lipid peroxidation increased during the course of embryogenesis, as does the amount of GST proteins. On the other hand, the GST genes have high expression at the 1st day post-oviposition, during the early stage of embryogenesis and at day 10 during the period wherein the germ band is observable. Fer gene expression also starts to increase at day 10 and peaks at day 15. In the ferritin proteins, only the secretory ferritin (Fer2) is detected and constitutively expressed during embryogenesis. Events occurring during embryogenesis, such as energy production and iron metabolism for cellular proliferation and differentiation cause oxidative stress in the embryo. To counteract oxidative stress, it is possible that the embryo may utilize oxidative stress-related molecules such as GSTs and Fer2, which could be either maternally or embryo-derived.
A Peroxiredoxin From the Haemaphysalis longicornis Tick Affects Langat Virus Replication in a Hamster Cell Line. International-journal Peer-reviewed

Kodai Kusakisako, Haruki Morokuma, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Kentaro Yoshii, Tetsuya Tanaka

Frontiers in cellular and infection microbiology　10　7-7　2020

DOI： 10.3389/fcimb.2020.00007 　

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Ticks are hematophagous arthropods, and their blood feeding on vertebrate hosts is essential for their development. The vertebrate blood contains high levels of free iron that can react with oxygen in ticks, resulting in the production of hydrogen peroxide (H2O2), one of the reactive oxygen species. Peroxiredoxins (Prxs), H2O2-scavenging enzymes, take on an important role in the ticks' oxidative stress coping mechanism. Ticks also transmit several disease-causing pathogens, including tick-borne encephalitis virus (TBEV), in animals and humans. Therefore, the control of ticks and tick-borne pathogens is a key issue that needs to be addressed. Infection with an arthropod-borne flavivirus is known to induce oxidative stress in insect cells. We hypothesize that vector-derived Prxs could have an effect on the infection and/or replication of flaviviruses in the hosts, since ticks Prxs are possibly transmitted from ticks to their hosts. In this study, we established stable strains of baby hamster kidney (BHK) cells expressing two types of H2O2-scavenging Prxs from the hard tick Haemaphysalis longicornis (BHK-HlPrx and BHK-HlPrx2 cells). Although the infection of TBEV surrogate Langat virus (LGTV) did not induce H2O2 production in normal BHK cells, the mortality rate and the virus titer of LGTV infected BHK-HlPrx cells increased. In addition, HlPrx proteins in BHK cells can facilitate LGTV replication in cells, while HlPrx2 proteins in BHK cells cannot. The results also demonstrated that this facilitation of LGTV replication by the 1-Cys Prx in the BHK cells is not by scavenging H2O2 but by an unknown mechanism. In order to understand this mechanism, more studies using tick-derived cells and ticks are necessary.
The case for oxidative stress molecule involvement in the tick-pathogen interactions -an omics approach. Peer-reviewed

Hernandez EP, Talactac MR, Fujisaki K, Tanaka T

Developmental and comparative immunology　100　103409　2019/11

DOI： 10.1016/j.dci.2019.103409 　

ISSN： 0145-305X
Characterization of an iron-inducible Haemaphysalis longicornis tick-derived promoter in an Ixodes scapularis-derived tick cell line (ISE6). International-journal Peer-reviewed

Hernandez EP, Kusakisako K, Hatta T, Tanaka T

Parasites & vectors　12　(1)　321-321　2019/06/25

DOI： 10.1186/s13071-019-3574-9 　

ISSN： 1756-3305
Molecular detection of Anaplasma spp. In blood and milk of dairy cattle in the Philippines Peer-reviewed

Angelito Pangan, Dela Cruz, Remil Linggatong Galay, Kristina Andrea, Claveria Sandalo, Flor Marie, Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka

Turkish Journal of Veterinary and Animal Sciences　43　540-545　2019/01/01

DOI： 10.3906/vet-1903-27 　

ISSN： 1300-0128
Molecular detection of tick-borne pathogens in canine population and Rhipicephalus sanguineus (sensu lato) ticks from southern Metro Manila and Laguna, Philippines. Peer-reviewed

Galay RL, Manalo AAL, Dolores SLD, Aguilar IPM, Sandalo KAC, Cruz KB, Divina BP, Andoh M, Masatani T, Tanaka T

Parasites & vectors　11　(1)　643　2018/12/17

DOI： 10.1186/s13071-018-3192-y 　

ISSN： 1756-3305
A Continuing Exploration of Tick-Virus Interactions Using Various Experimental Viral Infections of Hard Ticks. Peer-reviewed

Talactac MR, Hernandez EP, Fujisaki K, Tanaka T

Frontiers in physiology　9　1728　2018/12/04

DOI： 10.3389/fphys.2018.01728 　

ISSN： 1664-042X
Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6). International-journal Peer-reviewed

Emmanuel Pacia Hernandez, Kodai Kusakisako, Melbourne Rio Talactac, Remil Linggatong Galay, Kentaro Yoshii, Tetsuya Tanaka

Scientific reports　8　(1)　16566-16566　2018/11/08

DOI： 10.1038/s41598-018-34860-3 　

ISSN： 2045-2322
Transcriptional activities of two newly identified Haemaphysalis longicornis tick-derived promoter regions in the Ixodes scapularis tick cell line (ISE6) Peer-reviewed

Kusakisako K, Ido A, Masatani T, Morokuma H, Hernandez EP, Talactac MR, Yoshii K, Tanaka T

Insect Molecular Biology　27　(5)　590-602　2018/10

DOI： 10.1111/imb.12497 　

ISSN： 0962-1075
Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis. International-journal Peer-reviewed

Hernandez EP, Kusakisako K, Talactac MR, Galay RL, Hatta T, Fujisaki K, Tsuji N, Tanaka T

Parasites & vectors　11　(1)　460-460　2018/08/09

DOI： 10.1186/s13071-018-3044-9 　

ISSN： 1756-3305
Hemolymph defensin from the hard tick Haemaphysalis longicornis attacks Gram-positive bacteria. Peer-reviewed

Yada Y, Talactac MR, Kusakisako K, Hernandez EP, Galay RL, Andoh M, Fujisaki K, Tanaka T

Journal of invertebrate pathology　156　14-18　2018/07

DOI： 10.1016/j.jip.2018.07.005 　

ISSN： 0022-2011
The multiple roles of peroxiredoxins in tick blood feeding. Peer-reviewed

Kusakisako K, Fujisaki K, Tanaka T

Experimental & applied acarology　75　(3)　269-280　2018/07

DOI： 10.1007/s10493-018-0273-8 　

ISSN： 0168-8162
Vector competence of Haemaphysalis longicornis ticks for a Japanese isolate of the Thogoto virus. International-journal Peer-reviewed

Melbourne Rio Talactac, Kentaro Yoshii, Emmanuel Pacia Hernandez, Kodai Kusakisako, Remil Linggatong Galay, Kozo Fujisaki, Masami Mochizuki, Tetsuya Tanaka

Scientific reports　8　(1)　9300-9300　2018/06/18

DOI： 10.1038/s41598-018-27483-1 　

ISSN： 2045-2322
Peroxiredoxins are important for the regulation of hydrogen peroxide concentrations in ticks and tick cell line Peer-reviewed

Kodai Kusakisako, Emmanuel Pacia Hernandez, Melbourne Rio Talactac, Kentaro Yoshii, Rika Umemiya-Shirafuji, Kozo Fujisaki, Tetsuya Tanaka

Ticks and Tick-borne Diseases　9　(4)　872-881　2018/05

DOI： 10.1016/j.ttbdis.2018.03.016 　

ISSN： 1877-959X
Immunofluorescent detection in the ovary of host antibodies against a secretory ferritin injected into female Haemaphysalis longicornis ticks Peer-reviewed

Remil Linggatong Galay, Tomohide Matsuo, Emmanuel Pacia Hernandez, Melbourne Rio Talactac, Kodai Kusakisako, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

Parasitology International　67　(2)　119-122　2018/04/01

DOI： 10.1016/j.parint.2017.10.006 　

ISSN： 1873-0329 1383-5769
Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feeding Peer-reviewed

Emmanuel Pacia Hernandez, Kodai Kusakisako, Melbourne Rio Talactac, Remil Linggatong Galay, Takeshi Hatta, Tomohide Matsuo, Kozo Fujisaki, Naotoshi Tsuji, Tetsuya Tanaka

Parasites and Vectors　11　(1)　91　2018/02/08

DOI： 10.1186/s13071-018-2667-1 　

ISSN： 1756-3305
Evaluation of vaccine potential of 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis. International-journal Peer-reviewed

Kodai Kusakisako, Takeshi Miyata, Masashi Tsujio, Remil Linggatong Galay, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Kozo Fujisaki, Tetsuya Tanaka

Experimental & applied acarology　74　(1)　73-84　2018/01

DOI： 10.1007/s10493-018-0209-3 　

ISSN： 0168-8162
マダニペルオキシレドキシンの役割特に吸血における抗酸化機構と宿主の免疫応答について

草木迫浩大, 藤崎幸藏, 田仲哲也

獣医寄生虫学会誌　16　(1-2)　22-28　2017/12
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
The perspective of ferritin which regulates iron metabolism on tick

田仲哲也, ガライレミル, リングガートン, 白藤(梅宮) 梨可, 藤﨑幸蔵

細胞　49　(14)　715-719　2017/12
Publisher: ニューサイエンス社
ISSN： 1346-7557
フタトゲチマダニヘモリンフ由来ディフェンシンの同定とその抗菌作用

屋田友里花, 草木迫浩大, Talactac Melbourne R., Hernandez Emmanuel P., 前田大輝, 安藤匡子, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　68　(2)　75-75　2017/07
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
Synchronous Langat Virus Infection of Haemaphysalis longicornis Using Anal Pore Microinjection Peer-reviewed

Melbourne Rio Talactac, Kentaro Yoshii, Emmanuel Pacia Hernandez, Kodai Kusakisako, Remil Linggatong Galay, Kozo Fujisaki, Masami Mochizuki, Tetsuya Tanaka

VIRUSES-BASEL　9　(7)　2017/07

DOI： 10.3390/v9070189 　

ISSN： 1999-4915
Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis Peer-reviewed

Melbourne Rio Talactac, Yurika Yada, Kentaro Yoshii, Emmanuel Pacia Hernandez, Kodai Kusakisako, Hiroki Maeda, Remil Linggatong Galay, Kozo Fujisaki, Masami Mochizuki, Tetsuya Tanaka

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY　68　98-107　2017/03

DOI： 10.1016/j.dci.2016.11.013 　

ISSN： 0145-305X

eISSN： 1879-0089
Initial development of Babesia ovata in the tick midgut Peer-reviewed

Hiroki Maeda, Takeshi Hatta, M. Abdul Alim, Daigo Tsubokawa, Fusako Mikami, Kodai Kusakisako, Makoto Matsubayashi, Rika Umemiya-Shirafuji, Naotoshi Tsuji, Tetsuya Tanaka

VETERINARY PARASITOLOGY　233　39-42　2017/01

DOI： 10.1016/j.vetpar.2016.11.020 　

ISSN： 0304-4017

eISSN： 1873-2550
Detection and molecular characterization of Babesia, Theileria, and Hepatozoon species in hard ticks collected from Kagoshima, the southern region in Japan Peer-reviewed

Tatsunori Masatani, Kei Hayashi, Masako Andoh, Morihiro Tateno, Yasuyuki Endo, Masahito Asada, Kodai Kusakisako, Tetsuya Tanaka, Mutsuyo Gokuden, Nodoka Hozumi, Fumiko Nakadohzono, Tomohide Matsuo

TICKS AND TICK-BORNE DISEASES　8　(4)　581-587　2017

DOI： 10.1016/j.ttbdis.2017.03.007 　

ISSN： 1877-959X

eISSN： 1877-9603
Establishment of a novel tick-Babesia experimental infection model Peer-reviewed

Hiroki Maeda, Takeshi Hatta, M. Abdul Alim, Daigo Tsubokawa, Fusako Mikami, Makoto Matsubayashi, Takeharu Miyoshi, Rika Umemiya-Shirafuji, Shin-ichiro Kawazu, Ikuo Igarashi, Masami Mochizuki, Naotoshi Tsuji, Tetsuya Tanaka

SCIENTIFIC REPORTS　6　37039　2016/11

DOI： 10.1038/srep37039 　

ISSN： 2045-2322
Improvement of the cryopreservation method for the Babesia gibsoni parasite by using commercial freezing media Peer-reviewed

Kodai Kusakisako, Tatsunori Masatani, Yurika Yada, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Hiroki Maeda, Masami Mochizuki, Tetsuya Tanaka

PARASITOLOGY INTERNATIONAL　65　(5)　532-535　2016/10

DOI： 10.1016/j.parint.2016.02.012 　

ISSN： 1383-5769
2-Cys peroxiredoxin is required in successful blood-feeding, reproduction, and antioxidant response in the hard tick Haemaphysalis longicornis Peer-reviewed

Kodai Kusakisako, Remil Linggatong Galay, Rika Umemiya-Shirafuji, Emmanuel Pacia Hernandez, Hiroki Maeda, Melbourne Rio Talactac, Naotoshi Tsuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PARASITES & VECTORS　9　457　2016/08

DOI： 10.1186/s13071-016-1748-2 　

ISSN： 1756-3305
Impaired cellular immune response to injected bacteria after knockdown of ferritin genes in the hard tick Haemaphysalis longicornis Peer-reviewed

Remil Linggatong Galay, Rie Takechi, Rika Umemiya-Shirafuji, Melbourne Rio Talactac, Hiroki Maeda, Kodai Kusakisako, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PARASITOLOGY INTERNATIONAL　65　(3)　251-257　2016/06

DOI： 10.1016/j.parint.2016.01.007 　

ISSN： 1383-5769
鹿児島県屋久島のヒゲナガチマダニから分離されたリケッチアHKT-1株の遺伝子解析

安藤匡子, 本田俊郎, 御供田睦代, 藤田博己, 矢野泰弘, 正谷達謄, 田仲哲也, 松尾智英, 遠藤泰之, 安藤秀二, 川端寛樹

衛生動物　67　(Suppl.)　91-91　2016/04
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
A novel C-type lectin with triple carbohydrate recognition domains has critical roles for the hard tick Haemaphysalis longicornis against Gram-negative bacteria Peer-reviewed

Hiroki Maeda, Takeshi Miyata, Kodai Kusakisako, Remil Linggatong Galay, Melbourne Rio Talactac, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY　57　38-47　2016/04

DOI： 10.1016/j.dci.2015.12.015 　

ISSN： 0145-305X

eISSN： 1879-0089
Dynamics of Theileria orientalis genotype population in cattle in a year-round grazing system Peer-reviewed

Tatsunori Masatani, Shunpei Yoshihara, Atsuko Matsubara, Takafumi Gotoh, Hideyuki Takahashi, Tetsuya Tanaka, Masako Andoh, Yasuyuki Endo, Tomohide Matsuo

ACTA PARASITOLOGICA　61　(2)　419-424　2016/03

DOI： 10.1515/ap-2016-0056 　

ISSN： 1230-2821

eISSN： 1896-1851
Functional analysis of recombinant 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis Peer-reviewed

K. Kusakisako, T. Masatani, T. Miyata, R. L. Galay, H. Maeda, M. R. Talactac, N. Tsuji, M. Mochizuki, K. Fujisaki, T. Tanaka

INSECT MOLECULAR BIOLOGY　25　(1)　16-23　2016/02

DOI： 10.1111/imb.12193 　

ISSN： 0962-1075

eISSN： 1365-2583
Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus Peer-reviewed

Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Naotoshi Tsuji, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami Mochizuki

PARASITES & VECTORS　9　59　2016/02

DOI： 10.1186/s13071-016-1344-5 　

ISSN： 1756-3305
Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice Peer-reviewed

Yuina Ono, Makoto Matsubayashi, Hiroaki Kawaguchi, Masashi Tsujio, Masanobu Mizuno, Tetsuya Tanaka, Tatsunori Masatani, Toshihiro Matsui, Tomohide Matsuo

PARASITOLOGY RESEARCH　115　(1)　211-215　2016/01

DOI： 10.1007/s00436-015-4737-z 　

ISSN： 0932-0113

eISSN： 1432-1955
Role of the tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) from the hard tick Haemaphysalis longicornis in immunity against bacterial infection Peer-reviewed

Rie Takechi, Remil Linggatong Galay, Tomohide Matsuo, Hiroki Maeda, Kodai Kusakisako, Melbourne Rio Talactac, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

TICKS AND TICK-BORNE DISEASES　7　(1)　36-45　2016

DOI： 10.1016/j.ttbdis.2015.08.002 　

ISSN： 1877-959X

eISSN： 1877-9603
Induction of gene silencing in Haemaphysalis longicornis ticks through immersion in double-stranded RNA Peer-reviewed

Remil Linggatong Galay, Emmanuel Pacia Hernandez, Melbourne Rio Talactac, Hiroki Maeda, Kodai Kusakisako, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

TICKS AND TICK-BORNE DISEASES　7　(5)　813-816　2016

DOI： 10.1016/j.ttbdis.2016.03.018 　

ISSN： 1877-959X

eISSN： 1877-9603
Host Immunization with Recombinant Proteins to Screen Antigens for Tick Control. International-journal Peer-reviewed

Galay RL, Miyata T, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T

Methods in molecular biology (Clifton, N.J.)　1404　261-273　2016

DOI： 10.1007/978-1-4939-3389-1_18 　

ISSN： 1064-3745

More details Close

Ticks (Parasitiformes: Ixodida) are known for their obligate blood feeding habit and their role in transmitting pathogens to various vertebrate hosts. Tick control using chemical acaricides is extensively used particularly in livestock management, but several disadvantages arise from resistance development of many tick species, and concerns on animal product and environmental contamination. Vaccination offers better protection and more cost-effective alternative to application of chemical acaricides, addressing their disadvantages. However, an ideal anti-tick vaccine targeting multiple tick species and all the tick stages is still wanting. Here, we describe the procedures involved in the evaluation of a vaccine candidate antigen against ticks at the laboratory level, from the preparation of recombinant proteins, administration to the rabbit host and monitoring of antibody titer, to tick infestation challenge and determination of the effects of immunization to ticks.
The vector potential of Haemaphysalis longicornis ticks for Babesia microti parasites under experimental condition Peer-reviewed

K. Kusakisako, H. Maeda, R. L. Galay, T. Matsuo, M. Tsujio, R. Umemiya-Shirafuji, M. Mochizuki, K. Fujisaki, T. Tanaka

JOURNAL OF PROTOZOOLOGY RESEARCH　25　(1-2)　8-17　2015/12

ISSN： 0917-4427
Evaluation of Eimeria krijgsmanni as a murine model for testing the efficacy of anti-parasitic agents Peer-reviewed

Toshinori Takeo, Tetsuya Tanaka, Makoto Matsubayashi, Masashi Tsujio, Rika Umemiya-Shirafuji, Naotoshi Tsuji, Kozo Fujisaki, Toshihiro Matsui, Tomohide Matsuo

ACTA PARASITOLOGICA　60　(2)　190-195　2015/06

DOI： 10.1515/ap-2015-0027 　

ISSN： 1230-2821

eISSN： 1896-1851
Identification of the Babesia-responsive leucine-rich repeat domain-containing protein from the hard tick Haemaphysalis longicornis Peer-reviewed

Hiroki Maeda, Koshi Kurisu, Takeshi Miyata, Kodai Kusakisako, Remil Linggatong Galay, Talactac Melbourne Rio, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PARASITOLOGY RESEARCH　114　(5)　1793-1802　2015/05

DOI： 10.1007/s00436-015-4365-7 　

ISSN： 0932-0113

eISSN： 1432-1955
Iron metabolism in hard ticks (Acari: Ixodidae): The antidote to their toxic diet Peer-reviewed

Remil Linggatong Galay, Rika Umemiya-Shirafuji, Masami Mochizuld, Kozo Fujisald, Tetsuya Tanaka

PARASITOLOGY INTERNATIONAL　64　(2)　182-189　2015/04

DOI： 10.1016/j.parint.2014.12.005 　

ISSN： 1383-5769
The widely distributed hard tick, Haemaphysalis longicornis, can retain canine parvovirus, but not be infected in laboratory condition Peer-reviewed

Hiroyuki Mori, Tetsuya Tanaka, Masami Mochizuki

JOURNAL OF VETERINARY MEDICAL SCIENCE　77　(4)　405-411　2015/04

DOI： 10.1292/jvms.14-0199 　

ISSN： 0916-7250

eISSN： 1347-7439
Evaluation and comparison of the potential of two ferritins as anti-tick vaccines against Haemaphysalis longicornis Peer-reviewed

Remil Linggatong Galay, Takeshi Miyata, Rika Umemiya-Shirafuji, Hiroki Maeda, Kodai Kusakisako, Naotoshi Tsuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PARASITES & VECTORS　7　482　2014/10

DOI： 10.1186/s13071-014-0482-x 　

ISSN： 1756-3305
Molecular and phylogenetic characterizations of an Eimeria krijgsmanni Yakimoff & Gouseff, 1938 (Apicomplexa: Eimeriidae) mouse intestinal protozoan parasite by partial 18S ribosomal RNA gene sequence analysis Peer-reviewed

Toshinori Takeo, Tetsuya Tanaka, Makoto Matsubayashi, Hiroki Maeda, Kodai Kusakisako, Toshihiro Matsui, Masami Mochizuki, Tomohide Matsuo

PARASITOLOGY INTERNATIONAL　63　(4)　627-630　2014/08

DOI： 10.1016/j.parint.2014.04.004 　

ISSN： 1383-5769
Host specificity and in vivo infectivities of the mouse coccidian parasites Eimeria krijgsmanni Peer-reviewed

Kazuki Hashimoto, Tetsuya Tanaka, Makoto Matsubayashi, Kyoko Endo, Rika Umemiya-Shirafuji, Toshihiro Matsui, Tomohide Matsuo

ACTA PARASITOLOGICA　59　(2)　337-342　2014/06

DOI： 10.2478/s11686-014-0251-1 　

ISSN： 1230-2821

eISSN： 1896-1851
Expression analysis of autophagy-related genes in the hard tick Haemaphysalis longicornis Peer-reviewed

Rika Umemiya-Shirafuji, Remil Linggatong Galay, Hiroki Maeda, Suguru Kawano, Tetsuya Tanaka, Shinya Fukumoto, Hiroshi Suzuki, Naotoshi Tsuji, Kozo Fujisaki

VETERINARY PARASITOLOGY　201　(1-2)　169-175　2014/03

DOI： 10.1016/j.vetpar.2014.01.024 　

ISSN： 0304-4017

eISSN： 1873-2550
Two Kinds of Ferritin Protect Ixodid Ticks from Iron Overload and Consequent Oxidative Stress Peer-reviewed

Remil Linggatong Galay, Rika Umemiya-Shirafuji, Eugene T. Bacolod, Hiroki Maeda, Kodai Kusakisako, Jiro Koyama, Naotoshi Tsuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PLOS ONE　9　(3)　e90661　2014/03

DOI： 10.1371/journal.pone.0090661 　

ISSN： 1932-6203
Host-derived transferrin is maintained and transferred from midgut to ovary in Haemaphysalis longicomis ticks Peer-reviewed

Hiroyuki Mori, Remil Linggatong Galay, Hiroki Maeda, Tomohide Matsuo, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

TICKS AND TICK-BORNE DISEASES　5　(2)　121-126　2014

DOI： 10.1016/j.ttbdis.2013.09.004 　

ISSN： 1877-959X

eISSN： 1877-9603
TgGRA23, a novel Toxoplasma gondii dense granule protein associated with the parasitophorous vacuole membrane and intravacuolar network Peer-reviewed

Tatsunori Masatani, Tomohide Matsuo, Tetsuya Tanaka, Mohamad Alaa Terkawi, Eung-Goo Lee, Youn-Kyoung Goo, Gabriel Oluga Aboge, Junya Yamagishi, Kei Hayashi, Kyohko Kameyama, Shinuo Cao, Yoshifumi Nishikawa, Xuenan Xuan

PARASITOLOGY INTERNATIONAL　62　(4)　372-379　2013/08

DOI： 10.1016/j.parint.2013.04.003 　

ISSN： 1383-5769
Inhibitory effect of cyclophilin A from the hard tick Haemaphysalis longicornis on the growth of Babesia bovis and Babesia bigemina Peer-reviewed

Hiroki Maeda, Damdinsuren Boldbaatar, Kodai Kusakisako, Remil Linggatong Galay, Kyaw Min Aung, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

PARASITOLOGY RESEARCH　112　(6)　2207-2213　2013/06

DOI： 10.1007/s00436-013-3390-7 　

ISSN： 0932-0113
Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis Peer-reviewed

Remil Linggatong Galay, Kyaw Min Aung, Rika Umemiya-Shirafuji, Hiroki Maeda, Tomohide Matsuo, Hiroaki Kawaguchi, Noriaki Miyoshi, Hiroshi Suzuki, Xuenan Xuan, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka

JOURNAL OF EXPERIMENTAL BIOLOGY　216　(10)　1905-1915　2013/05

DOI： 10.1242/jeb.081240 　

ISSN： 0022-0949
Target of rapamycin (TOR) controls vitellogenesis via activation of the S6 kinase in the fat body of the tick, Haemaphysalis longicornis Peer-reviewed

Rika Umemiya-Shirafuji, Damdinsuren Boldbaatar, Min Liao, Banzragch Battur, Md. Morshedur Rahman, Thasaneeya Kuboki, Remit Linggatong Galay, Tetsuya Tanaka, Kozo Fujisaki

INTERNATIONAL JOURNAL FOR PARASITOLOGY　42　(11)　991-998　2012/10

DOI： 10.1016/j.ijpara.2012.08.002 　

ISSN： 0020-7519

eISSN： 1879-0135
Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice Peer-reviewed

Longzheng Yu, Junya Yamagishi, Shoufa Zhang, Chunmei Jin, Gabriel Oluga Aboge, Houshuang Zhang, Guohong Zhang, Tetsuya Tanaka, Kozo Fujisaki, Yoshifumi Nishikawa, Xuenan Xuan

PARASITOLOGY INTERNATIONAL　61　(3)　481-486　2012/09

DOI： 10.1016/j.parint.2012.04.001 　

ISSN： 1383-5769
RNAi of the translation inhibition gene 4E-BP identified from the hard tick, Haemaphysalis longicornis, affects lipid storage during the off-host starvation period of ticks Peer-reviewed

Aiko Kume, Damdinsuren Boldbaatar, Yuko Takazawa, Rika Umemiya-Shirafuji, Tetsuya Tanaka, Kozo Fujisaki

PARASITOLOGY RESEARCH　111　(2)　889-896　2012/08

DOI： 10.1007/s00436-012-2915-9 　

ISSN： 0932-0113
Parasiticidal activity of Haemaphysalis longicornis longicin P4 peptide against Toxoplasma gondii Peer-reviewed

Tetsuya Tanaka, Hiroki Maeda, Tomohide Matsuo, Damdinsuren Boldbattar, Rika Umemiya-Shirafuji, Aiko Kume, Hiroshi Suzuki, Xuenan Xuan, Naotoshi Tsuji, Kozo Fujisaki

PEPTIDES　34　(1)　242-250　2012/03

DOI： 10.1016/j.peptides.2011.07.027 　

ISSN： 0196-9781
Akt is an essential player in regulating cell/organ growth at the adult stage in the hard tick Haemaphysalis longicornis Peer-reviewed

Rika Umemiya-Shirafuji, Toru Tanaka, Damdinsuren Boldbaatar, Tetsuya Tanaka, Kozo Fujisaki

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY　42　(3)　164-173　2012/03

DOI： 10.1016/j.ibmb.2011.12.001 　

ISSN： 0965-1748
HlSRB, a Class B Scavenger Receptor, Is Key to the Granulocyte-Mediated Microbial Phagocytosis in Ticks Peer-reviewed

Kyaw Min Aung, Damdinsuren Boldbaatar, Rika Umemiya-Shirafuji, Min Liao, Naotoshi Tsuji, Xuan Xuenan, Hiroshi Suzuki, Aiko Kume, Remil Linggatong Galay, Tetsuya Tanaka, Kozo Fujisaki

PLOS ONE　7　(3)　e33504　2012/03

DOI： 10.1371/journal.pone.0033504 　

ISSN： 1932-6203
Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaphysalis longicornis Peer-reviewed

Remil Linggatong Galay, Hiroki Maeda, Kyaw Min Aung, Rika Umemiya-Shirafuji, Xuenan Xuan, Ikuo Igarashi, Naotoshi Tsuji, Tetsuya Tanaka, Kozo Fujisaki

TROPICAL ANIMAL HEALTH AND PRODUCTION　44　(2)　343-348　2012/02

DOI： 10.1007/s11250-011-0027-7 　

ISSN： 0049-4747
Tick longicin implicated in the arthropod transmission of toxoplasma gondii Peer-reviewed

Tetsuya Tanaka, Hiroki Maeda, Remil Linggatong Galay, Damdinsuren Boldbattar, Rika Umemiya-Shirafuji, Hiroshi Suzuki, Xuenan Xuan, Naotoshi Tsuji, Kozo Fujisaki

Journal of Veterinary Science and Technology　3　(2)　2012

DOI： 10.4172/2157-7579.1000112 　

ISSN： 2157-7579
Scavenger Receptor Mediates Systemic RNA Interference in Ticks Peer-reviewed

Kyaw Min Aung, Damdinsuren Boldbaatar, Rika Umemiya-Shirafuji, Min Liao, Xuan Xuenan, Hiroshi Suzuki, Remil Linggatong Galay, Tetsuya Tanaka, Kozo Fujisaki

PLOS ONE　6　(12)　e28407　2011/12

DOI： 10.1371/journal.pone.0028407 　

ISSN： 1932-6203
Cloning and characterization of the autophagy-related gene 6 from the hard tick, Haemaphysalis longicornis Peer-reviewed

Suguru Kawano, Rika Umemiya-Shirafuji, Damdinsuren Boldbaatar, Kenji Matsuoka, Tetsuya Tanaka, Kozo Fujisaki

PARASITOLOGY RESEARCH　109　(5)　1341-1349　2011/11

DOI： 10.1007/s00436-011-2429-x 　

ISSN： 0932-0113
Effects of Daily Oral Administration of Difructose Anhydride III on Health Status, Blood Parameters and Faecal Shedding of Coliform Bacteria of Japanese Black Calves during the Pre-weaning Period Peer-reviewed

M. Takagi, H. Hasunuma, D. Matsumoto, T. Obi, K. Takase, M. Ohtani, T. Sato, U. Watanabe, K. Okamoto, T. Tanaka, C. Tshering, E. Deguchi

ANIMAL NUTRITION AND FEED TECHNOLOGY　11　(2)　147-158　2011/07

ISSN： 0972-2963
ウシラクトペルオキシダーゼのin vitroでの細胞傷害防止効果について

石川稿大郎, 田仲哲也, 島崎敬一

ミルクサイエンス　60　(1)　1-6　2011/04
Publisher: 日本酪農科学会
ISSN： 1343-0289
Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis Peer-reviewed

Kyaw Min Aung, Damdinsuren Boldbaatar, Min Liao, Rika Umemiya-Shirafuji, Sumihiro Nakao, Terushige Matsuoka, Tetsuya Tanaka, Kozo Fujisaki

PARASITOLOGY RESEARCH　108　(2)　273-285　2011/02

DOI： 10.1007/s00436-010-2053-1 　

ISSN： 0932-0113
Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development Peer-reviewed

Damdinsuren Boldbaatar, Rika Umemiya-Shirafuji, Min Liao, Tetsuya Tanaka, Xuenan Xuan, Kozo Fujisaki

JOURNAL OF INSECT PHYSIOLOGY　56　(11)　1587-1598　2010/11

DOI： 10.1016/j.jinsphys.2010.05.019 　

ISSN： 0022-1910

eISSN： 1879-1611
Parasiticidal activity of human alpha-defensin-5 against Toxoplasma gondii Peer-reviewed

Tetsuya Tanaka, Md Morshedur Rahman, Banzragch Battur, Damdinsuren Boldbaatar, Min Liao, Rika Umemiya-Shirafuji, Xuenan Xuan, Kozo Fujisaki

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL　46　(6)　560-565　2010/06

DOI： 10.1007/s11626-009-9271-9 　

ISSN： 1071-2690

eISSN： 1543-706X
Structural Characterization and Cytolytic Activity of a Potent Antimicrobial Motif in Longicin, a Defensin-Like Peptide in the Tick Haemaphysalis longicornis Peer-reviewed

Md. Morshedur Rahman, Naotoshi Tsuji, Damdinsuren Boldbaatar, Banzragch Battur, Min Liao, Rika Umemiya-Shirafuji, Myungjo You, Tetsuya Tanaka, Kozo Fujisaki

JOURNAL OF VETERINARY MEDICAL SCIENCE　72　(2)　149-156　2010/02

DOI： 10.1292/jvms.09-0167 　

ISSN： 0916-7250
GATA transcription, translation and regulation in Haemaphysalis longicornis tick: Analysis of the cDNA and an essential role for vitellogenesis Peer-reviewed

Damdinsuren Boldbaatar, Banzragch Battur, Rika Umemiya-Shirafuji, Min Liao, Tetsuya Tanaka, Kozo Fujisaki

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY　40　(1)　49-57　2010/01

DOI： 10.1016/j.ibmb.2009.12.009 　

ISSN： 0965-1748

eISSN： 1879-0240
The identification and characterization of lysozyme from the hard tick Haemaphysalis longicornis Peer-reviewed

Tetsuya Tanaka, Suguru Kawano, Sumihiro Nakao, Rika Umemiya-Shirafuji, Md. Morshedur Rahman, Damdinsuren Boldbaatar, Banzragch Battur, Min Liao, Kozo Fujisaki

TICKS AND TICK-BORNE DISEASES　1　(4)　178-185　2010

DOI： 10.1016/j.ttbdis.2010.09.001 　

ISSN： 1877-959X

eISSN： 1877-9603
Effects of Oral Administration of Difructose Anhydride III on Selected Health and Blood Parameters of Group-housed Japanese Black Calves during the Preweaning Period Peer-reviewed

Daisaku Matsumoto, Mitsuhiro Takagi, Hiroshi Hasunuma, Yasuo Fushimi, Masayuki Ohtani, Tadashi Sato, Koji Okamoto, Francis Shahada, Tetsuya Tanaka, Eisaburo Deguchi

ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　22　(12)　1640-1647　2009/12

DOI： 10.5713/ajas.2009.90199 　

ISSN： 1011-2367

eISSN： 1976-5517
Activation mechanism of recombinant Der p 3 allergen zymogen - Contribution of cysteine protease Der p 1 and effect of propeptide glycosylation Peer-reviewed

Marie-Eve Dumez, Nathalie Teller, Frederic Mercier, Tetsuya Tanaka, Isabel Vandenberghe, Michel Vandenbranden, Bart Devreese, Andre Luxen, Jean-Marie Frere, Andre Matagne, Alain Jacquet, Moreno Galleni, Andy Chevigne

JOURNAL OF BIOLOGICAL CHEMISTRY　283　(45)　30606-30617　2008/11

DOI： 10.1074/jbc.M803041200 　

ISSN： 0021-9258
Expression and characterization of bovine lactoperoxidase by recombinant vaccinia virus Peer-reviewed

Tetsuya Tanaka, Xuenan Xuan, Asato Kojima, Ikuo Igarashi, Kozo Fujisaki, Kei-ichi Shimazaki

CYTOTECHNOLOGY　58　(3)　127-133　2008/11

DOI： 10.1007/s10616-009-9181-4 　

ISSN： 0920-9069
Identification of two forms of cyclophilin from the hard tick Haemaphysalis longicornis Peer-reviewed

Damdinsuren Boldbaatar, Robert Musyoka Kilonzo, Banzragch Battur, Rika Umemiya, Min Liao, Tetsuya Tanaka, Xuenan Xuan, Kozo Fujisaki

PROCESS BIOCHEMISTRY　43　(6)　615-625　2008/06

DOI： 10.1016/j.procbio.2008.01.026 　

ISSN： 1359-5113
DiC14-amidine cationic liposomes stimulate myeloid dendritic cells through Toll-like receptor 4 Peer-reviewed

Tetsuya Tanaka, Amandine Legat, Emmanuelle Adam, Jonathan Steuve, Jean-Stephane Gatot, Michel Vandenbranden, Liliana Ulianov, Caroline Lonez, Jean-Marie Ruysschaert, Eric Muraille, Marcel Tuynder, Michel Goldman, Alain Jacquet

EUROPEAN JOURNAL OF IMMUNOLOGY　38　(5)　1351-1357　2008/05

DOI： 10.1002/eji.200737998 　

ISSN： 0014-2980
Functional analysis of protein disulfide isomerases in blood feeding, viability and oocyte development in Haemaphysalis longicornis ticks Peer-reviewed

Min Liao, Daindinsuren Boldbaatar, Haiyan Gong, Penglong Huang, Rika Umemiya, Thasaneeya Harnnoi, Jinlin Zhou, Tetsuya Tanaka, Hiroshi Suzuki, Xuenan Xuan, Kozo Fujisaki

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY　38　(3)　285-295　2008/03

DOI： 10.1016/j.ibmb.2007.11.006 　

ISSN： 0965-1748
Profiles of bovine lactoferrin in the gastrointestinal tracts of rats as observed by ELISA, Western blotting and SELDI-affinity MS Peer-reviewed

Ran E. Yoshise, M. Matsumoto, H. Chiji, H. Kuwata, K. Shin, K. Yamauchi, Y. Tamura, T. Tanaka, H. Kumura, K. Shimazaki

MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL　62　(4)　446-450　2007

ISSN： 0026-3788
Reactivity of synthetic SAG1 (p30) peptide sequences with RH, S273 and beverley strain-induced anti-Toxoplasma gondii antibodies Peer-reviewed

M. Kato, F. G. Claveria, Y. Maki, K. Sanda, T. Tanaka, Y. Omata, H. Nagasawa, N. Suzuki

PATHOBIOLOGY　74　(1)　50-56　2007

DOI： 10.1159/000101051 　

ISSN： 1015-2008
Parasiticidal activity of bovine lactoperoxidase against Toxoplasma gondii Peer-reviewed

Tetsuya Tanaka, Shin Murakami, Haruto Kumura, Ikuo Igarashi, Kei-ichi Shimazaki

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE　84　(5)　774-779　2006/10

DOI： 10.1139/O06-142 　

ISSN： 0829-8211
Parasiticidal activity of bovine lactoperoxidase against Toxoplasma gondii

Tetsuya Tanaka, Shin Murakami, Haruto Kumura, Ikuo Igarashi, Kei-ichi Shimazaki

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE　84　(5)　774-779　2006/10

DOI： 10.1139/O06-142 　

ISSN： 0829-8211
Short report: Inhibitory effect of lactoferrin on in vitro growth of Babesia caballi Peer-reviewed

H Ikadai, T Tanaka, N Shibahara, H Tanaka, A Matsuu, N Kudo, K Shimazaki, Igarashi, I, T Oyamada

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE　73　(4)　710-712　2005/10

ISSN： 0002-9637
Toxoplasma gondii antigens GRA1 (p24) and SAG1 (p30): A comparison of their stimulatory influence on T-cell activation and cytokine expression in in vitro cultures

M Kato, FG Claveria, Y Maki, T Tanaka, N Suzuki, H Nagasawa

PATHOBIOLOGY　72　(3)　160-164　2005

DOI： 10.1159/000084120 　

ISSN： 1015-2008

eISSN： 1423-0291
Susceptibility of bovine osteopontin to chymosin Peer-reviewed

H Kumura, A Miura, E Sato, T Tanaka, K Shimazaki

JOURNAL OF DAIRY RESEARCH　71　(4)　500-504　2004/11

DOI： 10.1017/S0022029904000391 　

ISSN： 0022-0299
Susceptibilities against bovine lactoferrin with microorganisms isolated from mastitic milk Peer-reviewed

NY Lee, K Kawai, Nakamura, I, T Tanaka, H Kumura, K Shimazaki

JOURNAL OF VETERINARY MEDICAL SCIENCE　66　(10)　1267-1269　2004/10

DOI： 10.1292/jvms.66.1267 　

ISSN： 0916-7250
Detection of bovine lactoferrin binding protein on Jurkat human lymphoblastic T cell line Peer-reviewed

T Tanaka, H Morita, YC Yoo, WS Kim, H Kumura, KI Shimazaki

JOURNAL OF VETERINARY MEDICAL SCIENCE　66　(7)　865-869　2004/07

DOI： 10.1292/jvms.66.865 　

ISSN： 0916-7250
Growth-promoting effects of lactoferrin on L-acidophilus and Bifidobacterium spp. Peer-reviewed

WS Kim, M Ohashi, T Tanaka, H Kumura, GY Kim, IK Kwon, JS Goh, K Shimazaki

BIOMETALS　17　(3)　279-283　2004/06

ISSN： 0966-0844
The detection of bovine lactoferrin binding protein on Trypanosoma brucei Peer-reviewed

T Tanaka, Y Abe, N Inoue, WS Kim, H Kumura, H Nagasawa, Igarashi, I, K Shimazaki

JOURNAL OF VETERINARY MEDICAL SCIENCE　66　(6)　619-625　2004/06

DOI： 10.1292/jvms.66.619 　

ISSN： 0916-7250
Transferrin family proteins bind to Bifidobacterium spp.

WS Kim, T Tanaka, K Shimazaki

MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL　59　(9-10)　491-494　2004

ISSN： 0026-3788
Detection of binding-protein in Bifidobacterium bifidum Bb-11 by various kinds of lactoferrin

WS Kim, T Tanaka, K Shimazaki

MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL　59　(7-8)　375-377　2004

ISSN： 0026-3788
Comparison of Reactivity of Polyclonal and Monoclonal Antibodies against Bovine Lactoferrin and its Fragments (ラクトフェリン研究--基礎から応用への掛け橋)

Tun Khin Mg, 田仲哲也, 玖村朗人

ミルクサイエンス　53　(4)　239-241　2004
Publisher: 日本酪農科学会
ISSN： 1343-0289
Partial characterization of dextran-degrading enzyme obtained from blue cheese

Y. Wang, A. Suzuki, T. Tanaka, H. Kumura, K. Shimazaki

Journal of Dairy Science　87　(6)　1627-1633　2004
Publisher: American Dairy Science Association
DOI： 10.3168/jds.S0022-0302(04)73317-2 　

ISSN： 0022-0302
Screening of dairy yeast strains for probiotic applications

H. Kumura, Y. Tanoue, M. Tsukahara, T. Tanaka, K. Shimazaki

Journal of Dairy Science　87　(12)　4050-4056　2004
Publisher: American Dairy Science Association
DOI： 10.3168/jds.S0022-0302(04)73546-8 　

ISSN： 0022-0302
The detection of bovine lactoferrin binding protein on Toxoplasma gondii Peer-reviewed

T Tanaka, Y Abe, WS Kim, Xuan, X, H Nagasawa, Igarashi, I, H Kumura, K Shimazaki

JOURNAL OF VETERINARY MEDICAL SCIENCE　65　(12)　1377-1380　2003/12

DOI： 10.1292/jvms.65.1377 　

ISSN： 0916-7250
Antiviral activity of lactoferrin against canine herpesvirus. International-journal Peer-reviewed

Tetsuya Tanaka, Syogo Nakatani, Xuenan Xuan, Haruto Kumura, Ikuo Igarashi, Kei ichi Shimazaki

Antiviral research　60　(3)　193-9　2003/11

DOI： 10.1016/S0166-3542(03)00157-8 　

ISSN： 0166-3542
Expression and characterization of bovine lactoperoxidase by recombinant baculovirus Peer-reviewed

T Tanaka, S Sato, H Kumura, K Shimazaki

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　67　(10)　2254-2261　2003/10

DOI： 10.1271/bbb.67.2254 　

ISSN： 0916-8451

eISSN： 1347-6947
Expression of bovine lactoferrin and lactoferrin N-lobe by recombinant baculovirus and its antimicrobial activity against Prototheca zopfii Peer-reviewed

T Tanaka, Nakamura, I, NY Lee, H Kumura, K Shimazaki

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE　81　(5)　349-354　2003/10

DOI： 10.1139/O03-062 　

ISSN： 0829-8211
Preliminary study and characterization of dextran degradation by the water-soluble fraction extracted from blue cheese Peer-reviewed

K Shimazaki, S Maki, Y Wang, M Sato, T Tanaka, H Kumura

MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL　58　(7-8)　379-382　2003

ISSN： 0026-3788
Casein digestion by Debaryomyces hansenii isolated from cheese Peer-reviewed

H Kumura, K Takagaki, T Sone, M Tsukahara, T Tanaka, K Shimazaki

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　66　(6)　1370-1373　2002/06

DOI： 10.1271/bbb.66.1370 　

ISSN： 0916-8451

eISSN： 1347-6947
Fine structures of epitopic sites in human and bovine lactoferrin recognized by anti-bovine lactoferrin C-lobe monoclonal antibody

MS Nam, M Kamio, KI Shimazaki, S Harakawa, T Tanaka, Y Omata, A Saito, H Kumura, Igarashi, I, N Suzuki

FOOD AND AGRICULTURAL IMMUNOLOGY　14　(2)　139-146　2002/06

DOI： 10.1080/09540100220145160 　

ISSN： 0954-0105
Lactoferrin-binding proteins in Bifidobacterium bifidum Peer-reviewed

WS Kim, T Tanaka, H Kumura, K Shimazaki

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE　80　(1)　91-94　2002/02

ISSN： 0829-8211

eISSN： 1208-6002
Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1 Peer-reviewed

XN Xuan, Igarashi, I, T Tanaka, S Fukumoto, H Nagasawa, K Fujisaki, T Mikami

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY　8　(3)　645-646　2001/05

DOI： 10.1128/CDLI.8.3.645-646.2001 　

ISSN： 1071-412X
Expression of Babesia equi merozoite antigen 1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay

XA Xuan, A Larsen, H Ikadai, T Tanaka, Igarashi, I, H Nagasawa, K Fujisaki, Y Toyoda, N Suzuki, T Mikami

JOURNAL OF CLINICAL MICROBIOLOGY　39　(2)　705-709　2001/02

DOI： 10.1128/JCM.39.2.705-709.2001 　

ISSN： 0095-1137
The role of CD4(+) or CD8(+) T cells in the protective immune response of BALB/c mice to Neospora caninum infection

T Tanaka, T Hamada, N Inoue, H Nagasawa, K Fujisaki, N Suzuki, T Mikami

VETERINARY PARASITOLOGY　90　(3)　183-191　2000/06

DOI： 10.1016/S0304-4017(00)00238-7 　

ISSN： 0304-4017
Growth-inhibitory effects of interferon-γ on Neospora caninum in murine macrophages by a nitric oxide mechanism

Tetsuya Tanaka, Hideyuki Nagasawa, Kozo Fujisaki, Naoyoshi Suzuki, Takeshi Mikami

Parasitology Research　86　(9)　768-771　2000
Publisher: Springer Verlag
DOI： 10.1007/s004360000242 　

ISSN： 0932-0113
Detection of Antibodies to Babesia equi by the Latex Agglutination Test with Recombinant Merozoite Antigen-2

T. Tanaka, X. Xuan, I. Igarashi, H. Nagasawa, K. Fujisaki, N. Suzuki, T. Mikami

Journal of Protozoology Research　9　152-156　1999/12/01

ISSN： 0917-4427
Expression of Recombinant Toxoplasma gondii P24 Peer-reviewed

Tetsuya Tanaka, Xuenan Xuan, Mihoko Kato, Hideyuki Nagasawa, Kozo Fujisaki, Takeshi Mikami, Naoyoshi Suzuki

Journal of Veterinary Medical Science　61　(11)　1235-1239　1999/11

ISSN： 1782-155X
Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA Peer-reviewed

Tetsuya Tanaka, Xuenan Xuan, Hiromi Ikadai, Ikuo Igarashi, Hideyuki Nagasawa, Kozo Fujisaki, Takeshi Mikami, Naoyoshi Suzuki

International Journal for Parasitology　29　(11)　1803-1808　1999/11

DOI： 10.1016/S0020-7519(99)00146-0 　

ISSN： 0020-7519
Expression of recombinant Toxoplasma gondii P24

T Tanaka, Xuan, X, M Kato, H Nagasawa, K Fujisaki, T Mikami, N Suzuki

JOURNAL OF VETERINARY MEDICAL SCIENCE　61　(11)　1235-1239　1999/11

ISSN： 0916-7250

eISSN： 1347-7439
Growth Inhibitory Effect of Bovine Lactoferrin to Toxoplasma gondii Tachyzoites in Murine Macrophages: Tyrosine Phosphorylation in Murine Macrophages Induced by Bovine Lactoferrin

Tetsuya Tanaka, Yoshitaka Omata, Tsuya Isamida, Atsushi Saito, Keiichi Shimazaki, Koji Yamauchi, Naoyoshi Suzuki

Journal of Veterinary Medical Science　60　(3)　369-371　1998
Publisher: Maruzen Co. Ltd.
DOI： 10.1292/jvms.60.369 　

ISSN： 0916-7250
Protective Effect of Lactoferricin against Toxoplasma gondii Infection in Mice

Tsuya Isamida, Tetsuya Tanaka, Yoshitaka Omata, Koji Yamauchi, Kei-Ichi Shimazaki, Atsushi Saito

Journal of Veterinary Medical Science　60　(2)　241-244　1998
Publisher: Maruzen Co. Ltd.
DOI： 10.1292/jvms.60.241 　

ISSN： 0916-7250
Structural and immunochemical studies on bovine lactoferrin fragments

K Shimazaki, M Kamio, MS Nam, S Harakawa, T Tanaka, Y Omata, A Saito, H Kumura, K Mikawa, Igarashi, I, N Suzuki

ADVANCES IN LACTOFERRIN RESEARCH　443　41-48　1998

ISSN： 0065-2598
Growth inhibitory effect of bovine lactoferrin on Toxoplasma gondii tachyzoites in murine macrophages: Role of radical oxygen and inorganic nitrogen oxide in Toxoplasma growth-inhibitory activity

T Tanaka, Y Omata, M Narisawa, A Saito, K Shimazaki, Igarashi, I, H Hirumi, N Suzuki

VETERINARY PARASITOLOGY　68　(1-2)　27-33　1997/01

DOI： 10.1016/S0304-4017(96)01069-2 　

ISSN： 0304-4017
Monoclonal antibody against bovine Lactoferricin(R) and its epitopic site Peer-reviewed

K Shimazaki, MS Nam, S Harakawa, T Tanaka, Y Omata, A Saito, H Kumura, K Mikawa, Igarashi, I, N Suzuki

JOURNAL OF VETERINARY MEDICAL SCIENCE　58　(12)　1227-1229　1996/12

ISSN： 0916-7250
ウシラクトフェリンのToxoplasma gondii増殖抑制効果 Peer-reviewed

田仲哲也, 小俣吉孝, 齋藤篤志

The Journal of Veterinary Medical Science　58　(1)　61-65　1996/01/25
Publisher: (公社)日本獣医学会
ISSN： 0916-7250

More details Close

Lactoferrin (LF) is known to have broad spectrum antimicrobial properties. In regards to its defense mechanism against parasitic infection, it has shown phagocytic activity in the destruction of amastigotes, an intracellular parasitic form of Trypanosoma cruzi in macrophages. The effect of bovine lactoferrin on the intracellular growth Toxoplasma gondii parasites was examined in murine macrophage and embryonal cells. Co-cultures of host cells with the parasites were supplemented with either lactoferrin, apo-lactoferrin, holo-lactoferrin or transferrin in the culture media for varying periods. The growth activity of intracellular parasites in the host cells was determined by the measurement of selective incorporation of ^3H-uracil. Supplement of lactoferrin had no effect on the penetration activity of the parasites, while development of intracellular parasites was inhibited linearly in concentration of lactoferrin. Supplement of apo-lactoferrin and holo-lactoferrin, but not transferrin showed similar effects. These suggest that lactoferrin induces the inhibitory effects on the development of intracellular parasites. Pretreatment of lactoferrin to the macrophages, however, did not show any inhibitory effects. Whereas, mouse embryonal cells preincubated with lactoferrin suppressed the intracellular growth. Thus, the action of lactoferrin to macrophages would be different from that of mouse embryonal cells.
Toxoplasma gondii: Parasiticidal effects of bovine lactoferricin against parasites Peer-reviewed

Tetsuya Tanaka, Yoshitaka Omata, Atushi Saito, Keiichi Shimazaki, Koji Yamauchi, Mitsunori Takase, Kouzou Kawase, Ikuo Igarashi, Naoyoshi Suzuki

Experimental Parasitology　81　(4)　614-617　1995

DOI： 10.1006/expr.1995.1157 　

ISSN： 0014-4894
SEPARATION OF TRYPTIC FRAGMENTS OF BOVINE LACTOFERRIN AND THEIR ABILITY TO BIND TO TRYPANOSOMA-CRUZI Peer-reviewed

K SHIMAZAKI, T TANAKA, IGARASHI, I, A SAITO, H KANBARA, N SUZUKI

INDIGENOUS ANTIMICROBIAL AGENTS OF MILK - RECENT DEVELOPMENTS　122-130　1994
Separation and Characterization of the C-Terminal Half Molecule of Bovine Lactoferrin Peer-reviewed

Kei-Ichi Shimazaki, Tetsuya Tanaka, Hiroshi Kon, Kazunary Oota, Ayano Kawaguchi, Yoshiyuki Maki, Tetsuya Sato, Yukari Ueda, Toshio Tomimura, Seiichi Shimamura

Journal of Dairy Science　76　(4)　946-955　1993

DOI： 10.3168/jds.S0022-0302(93)77421-4 　

ISSN： 0022-0302

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土岐俊輔, 森下陽平, 本間悠人, 古村翔, 二神泰基, 田仲哲也, 尾崎太郎, 浅井禎吾

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東北畜産学会報　75　(2)　31-31　2025/08
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渡辺朋弥, 金井大地, 下田蒼, 米山裕, 田仲哲也, 戸部隆太

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金森萌, 間嶋朱里, 下田蒼, 松田敬一, 栗原達夫, 金子淳, 米山裕, 田仲哲也, 戸部隆太

日本農芸化学会大会講演要旨集(Web)　2025　2025

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間嶋朱里, 下田蒼, 松田敬一, 栗原達夫, 金子淳, 米山裕, 田仲哲也, 金森萌, 戸部隆太

日本農芸化学会大会講演要旨集(Web)　2025　2025

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Functional analysis of aquaporin in Haemaphysalis longicornis.

佐藤萌子, 佐藤成子, 水野寛太, 田仲哲也, XUAN X., 鈴木宏志, 白藤(梅宮)梨可

日本ダニ学会誌　33　(1)　2024

ISSN： 0918-1067
鹿児島県大隅地域におけるRickettsia spp.の疫学調査ならびにgltA遺伝子を標的とした分子系統解析

鈴一之将, 横山雅大, 芦原誠一, 榮村奈緒子, 田仲哲也

衛生動物　74　(2)　85-85　2023/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
Occurrence of ticks and the prevalence of Rickettsia species among different environments in the Osumi region of Kagoshima Prefecture

栄村奈緒子, 横山雅大, 畑邦彦, 芦原誠一, 鈴一之将, 田仲哲也

日本生態学会大会講演要旨(Web)　70th　2023
マダニ唾液由来HISG-g22様タンパク質の計算化学的解析

川田逸人, 川田逸人, 小池優貴, 佐々木紗己, 中島政斗, 佐々木萌可, 上松史尚, 三角修斗, KWOFIE Kofi Dadzie, LADZEKPO Danielle, LADZEKPO Danielle, BEHRI Meryem, 井上貴裕, 神保恵, 三上房子, 田仲哲也, 辻尚利, 辻尚利, 八田岳士, 八田岳士

日本寄生虫学会大会プログラム・抄録集　92nd　2023

ISSN： 1348-4613
Development of rapid genetic detection of small-sized piroplasmosis in cattle using recombinase polymerase amplification

樋ノ口峰大, 叶有斗, 志賀慧, 堀江真行, 一二三達郎, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　92nd　2023

ISSN： 1348-4613
Expression analysis of ferritins during the embryogenesis of the tick

島崎慧, HERNANDEZ Emmanuel, HERNANDEZ Emmanuel, 新原博子, 白藤(梅宮)梨可, 藤崎幸蔵, 田仲哲也

日本分子生物学会年会プログラム・要旨集(Web)　46th　2023
鹿児島県大隅地域の異なる環境におけるマダニ類の季節消長とリケッチア保有状況

榮村奈緒子, 鈴一之将, 横山雅大, 木村仁美, 芦原誠一, 畑邦彦, 一二三達郎, 田仲哲也

九州森林学会大会(Web)　79th　2023
日本の食用馬の多包虫症における多包条虫のミトコンドリア・シトクロームb(cob)遺伝子を標的とした分子系統解析

佐藤美穂, 一二三達郎, 田仲哲也, 鈴一之将, 秋岡幸兵, 藤又千晶, 新飼竜平, 前田勉, 草木迫浩大, 筏井宏実, 三好宣彰

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2023　2023
鹿児島県大隅地域から検出されたRickettsia spp.におけるgltA,ompB,htrA遺伝子の分子疫学的特徴

鈴一之将, 木村仁美, 芦原誠一, 榮村奈緒子, 一二三達郎, 田仲哲也

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2023　2023
マダニにおけるReMOT Controlの試み(Attempt of ReMOT Control for tick, Haemaphysalis longicornis)

Hernandez Emmanuel Pacia, 川田逸人, 辻尚利, 田仲哲也, 八田岳士

衛生動物　73　(Suppl.)　56-56　2022/03
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
Expression analysis of ferritins during the embryogenesis of the tick Haemaphysalis longicornis

島崎慧, ヘルナンデズエマヌエル, ヘルナンデズエマヌエル, 新原博子, 白藤(梅宮)梨可, 藤崎幸蔵, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　91st　2022

ISSN： 1348-4613
ダニ媒介性ウイルスのマダニ感染モデルの構築および感染・増殖機構の解析

田仲哲也, 好井健太朗

長崎大学熱帯医学研究拠点共同研究報告集　2021　2022
リコンビナーゼポリメラーゼ増幅法を用いた牛のTheileria orientalis遺伝子型の同定

樋ノ口峰大, 叶有斗, 志賀慧, 堀江真行, 一二三達郎, 田仲哲也

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2022 (CD-ROM)　2022
馬の多包虫症におけるリコンビナーゼポリメラーゼ増幅法を用いた迅速な診断法の開発

佐藤美穂, 佐藤美穂, 一二三達郎, 一二三達郎, 田仲哲也, 秋岡幸兵, 藤又千晶, 三好宣彰

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2022 (CD-ROM)　2022
Haemaphysalis longicornisに由来するelongation factor I-α(EFIα)のプロモータ分析(Promoter analysis of elongation factor I-alpha(EFI α) derived from Haemaphysalis longicornis)

Hernandez Emmanuel Pacia, 川田逸人, 山本大介, 加藤大智, 松林誠, 田仲哲也, 辻尚利, 八田岳士

日本寄生虫学会・日本臨床寄生虫学会合同大会プログラム・抄録集　90回・32回　58-58　2021/04
Publisher: 日本寄生虫学会・日本臨床寄生虫学会
馬の多包虫症におけるLAMP法を用いた迅速的な診断方法の開発

一二三達郎, 一二三達郎, 秋岡幸兵, 田仲哲也, 三好宣彰

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2021　2021
マダニフェリチンの機能解明

田仲哲也

衛生動物　72　(Supplement)　2021

ISSN： 0424-7086
ダニ研究の最前線とダニ媒介性感染症制御の可能性を探るマダニの吸血生理と抗マダニワクチン

田仲哲也

衛生動物　71　(2)　57-64　2020/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ由来カタラーゼ遺伝子の同定ならびに組換え体を用いた抗酸化活性の検証

草木迫浩大, 中尾亮, 田仲哲也, 野中成晃

衛生動物　71　(2)　113-113　2020/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
ランガットウイルスに感染したフタトゲチマダニにおける抗酸化分子の発現動態

山田智大, Pacia Hernandez Emmanuel, 新原博子, 藤崎幸蔵, 好井健太郎, 田仲哲也

衛生動物　71　(2)　132-132　2020/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ由来カタラーゼ遺伝子の同定ならびに組換え体を用いた抗酸化活性の検証

草木迫浩大, 中尾亮, 田仲哲也, 野中成晃

衛生動物　71　(2)　113-113　2020/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
単為生殖系フタトゲチマダニ胚発生における鉄イオンの動態と抗酸化分子の局在

島崎慧, Pacia Hernandez Emmanuel, 新原博子, 藤崎幸蔵, 田仲哲也

衛生動物　71　(2)　132-132　2020/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
ランガットウイルスに感染したフタトゲチマダニにおける抗酸化分子の発現動態

山田智大, Pacia Hernandez Emmanuel, 新原博子, 藤崎幸蔵, 好井健太郎, 田仲哲也

衛生動物　71　(2)　132-132　2020/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ伸長因子1α(EF1α)遺伝子の上流領域解析(Promoter analysis of elongation factor 1-alpha(EF1α) derived from Haemaphysalis longicornis tick)

エマニュエル・ヘルナンデス, コフィ・コフィ, 坪川大悟, 三上房子, 松林誠, 辻尚利, 田仲哲也, 八田岳士

衛生動物　71　(Suppl.)　52-52　2020/03
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ胚発生における鉄イオンの動態と抗酸化分子の局在

島崎慧, HERNANDEZ Emmanuel, 新原博子, 白藤(梅宮)梨可, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　163rd　2020

ISSN： 1347-8621
マダニ胚由来細胞におけるフタトゲチマダニプロモータ活性の評価

HERNANDEZ Emmanuel Pacia, HERNANDEZ Emmanuel Pacia, 草木迫浩大, 八田岳士, 田仲哲也

日本分子生物学会年会プログラム・要旨集(Web)　43rd　2020
グルタチオンSトランスフェラーゼはフタトゲチマダニの酸化ストレス管理とダニ駆除剤代謝に重要である(Glutathione S-transferases are important in oxidative stress management and acaricide metabolism in the hard ticks Haemaphysalis longicornis)

Hernandez Emmanuel, 草木迫浩大, Talactac Melbourne, Galay Remil, 八田岳士, 藤崎幸蔵, 辻尚利, 田仲哲也

日本獣医学会学術集会講演要旨集　162回　218-218　2019/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
単為生殖系フタトゲチマダニの胚発生における抗酸化分子の発現プロファイルの作成

島崎慧, Hernandez Emmanuel Pacia, 新原博子, 藤崎幸蔵, 田仲哲也

衛生動物　70　(2)　107-107　2019/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
フタトゲチマダニ由来抗酸化蛋白質を発現する哺乳類細胞の発現安定株の作製

諸熊遥樹, 草木迫浩大, Talactac Melbourne R., Hernandez Emmanuel P., 田仲哲也

衛生動物　70　(2)　107-107　2019/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
カナダから輸入された馬にみられた肝臓灰白色硬結節と多包虫感染との関連性

一二三達郎, 一二三達郎, 田仲哲也, HERNANDEZ Emmanuel Pacia, 秋岡幸兵, 山田薫理, 今村安孝, 畑井仁, 三好宣彰

日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)　2019　2019
哺乳類細胞におけるマダニアクチン遺伝子プロモーターを用いた外来遺伝子の発現

諸熊遥樹, 草木迫浩大, Talactac Melbourne R., Hernandez Emmanuel P., 田仲哲也

衛生動物　69　(2)　123-123　2018/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
マダニ胚由来細胞における遺伝子発現ベクターの探索並びに構築

田仲哲也, 井戸明子, 草木迫浩大, 諸熊遥樹, 正谷達謄, Hernandez Emmanuel, Talactac Melbourne, 好井健太朗

生命科学系学会合同年次大会　2017年度　[1P-1317]　2017/12
Publisher: 生命科学系学会合同年次大会運営事務局
マダニ‐バベシア実験感染モデルの確立

前田大輝, 前田大輝, 前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林誠, 三好猛晴, 白藤(梅宮, 梨可, 河津信一郎, 五十嵐郁男, 望月雅美, 辻尚利, 田仲哲也

日本獣医学会学術集会講演要旨集　160th　203-203　2017/08/30
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
シカダニIxodes scapularis胚由来細胞株(ISE6)におけるFerritin 1発現誘導(Induction of Ferritin1 expression in Ixodes scapularis embryo derived cell line(ISE6))

Hernandez Emmanuel, 草木迫浩大, Galay Remil, Talactac Melbourne, 好井健太朗, 田仲哲也

日本獣医学会学術集会講演要旨集　160回　346-346　2017/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニHaemaphysalis longicornisにおいて新規同定されたディフェンシン様ペプチドHEdefensinの性状解析と抗ウイルス活性(Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis)

Talactac Melbourne, 屋田友里花, 好井健太朗, Hernandez Emmanuel, 草木迫浩大, 前田大輝, 藤崎幸蔵, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　160回　390-390　2017/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
マダニペルオキシレドキシンの特性解明(ペルオキシレドキシンの性状解析並びに吸血・産卵に果たす役割について)

草木迫浩大, 正谷達謄, 宮田健, Galay Remil, 白藤梨可, 宮, 前田大輝, Talactac Melbourne, Hernandez Emmanuel, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　160回　202-202　2017/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
マダニ胚由来細胞における遺伝子発現ベクターの検証

井戸明子, 正谷達謄, 好井健太朗, Galay Remil L., 草木迫浩大, Talactac Melbourne R., Hernandez Emmanuel P., 望月雅美, 田仲哲也

衛生動物　68　(2)　75-75　2017/07
Publisher: 日本衛生動物学会
ISSN： 0424-7086

eISSN： 2185-5609
ペルオキシレドキシンは連携して細胞内過酸化水素濃度を制御する

草木迫浩大, HERNANDEZ Emmanuel P., 草木迫浩大, HERNANDEZ Emmanuel P., TALACTAC Melbourne R., TALACTAC Melbourne R., TALACTAC Melbourne R., 好井健太朗, 田仲哲也, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　86th　2017

ISSN： 1348-4613
The development of anti‐tick vaccine as a target against regulating molecule for oxidant stress

田仲哲也, 草木迫浩大, 宮田健

食肉に関する助成研究調査成果報告書　34　253‐257　2016/11
Babesia ovata原虫におけるマダニ中腸形態へのステージシフト誘導実験

前田大輝, 前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林誠, 白藤(梅宮)梨可, 辻尚利, 田仲哲也, 田仲哲也

日本獣医学会学術集会講演要旨集　159th　334-334　2016/08/30
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
鹿児島県各地で採取されたマダニにおけるマダニ媒介性原虫の分子疫学的解析

正谷達謄, 林慶, 立野守洋, 遠藤泰之, 松尾智英, 田仲哲也, 御供田睦代, 安藤匡子

日本獣医学会学術集会講演要旨集　159th　338-338　2016/08/30
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
マダニISE6細胞を用いた過酸化水素発生の可視化についての検討

草木迫浩大, Talactac Melbourne, Hernandez Emmanuel, 前田大輝, 好井健太朗, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　159回　339-339　2016/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
Haemaphysalis longicornisにおけるThogotoウイルス(HI-Kamigamo-25)日本単離株の生存動態(Survival dynamics of Japan isolate Thogoto virus(HI-Kamigamo-25) in Haemaphysalis longicornis)

Talactac Melbourne, 好井健太朗, Hernandez Emmanuel, 草木迫浩大, 前田大輝, 藤崎幸蔵, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　159回　383-383　2016/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
鹿児島県各地で採取されたマダニにおけるTheileria,Babesia及びHepatozoonの分子疫学

正谷達謄, 林慶, 立野守洋, 遠藤泰之, 松尾智英, 田仲哲也, 御供田睦代, 関(市川)まどか, 安藤匡子

日本寄生虫学会大会プログラム・抄録集　85th　83　2016/02

ISSN： 1348-4613
汎用的な細胞凍結保存液を用いたBabesia gibsoni原虫凍結保存法の検討およびその改良

草木迫浩大, 草木迫浩大, 正谷達謄, 屋田友里花, TALACTAC Melbourne Rio, TALACTAC Melbourne Rio, HERNANDEZ Emmanuel Pacia, 前田大輝, 前田大輝, 望月雅美, 望月雅美, 田仲哲也, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　85th　2016

ISSN： 1348-4613
フタトゲチマダニ体内におけるBabesia ovata移行動態について

前田大輝, 前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林誠, 三好猛晴, 白藤(梅宮)梨可, 大久保和洋, 五十嵐郁男, 望月雅美, 望月雅美, 藤崎幸蔵, 辻尚利, 田仲哲也, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　85th　2016

ISSN： 1348-4613
過酸化水素消去酵素2-Cysペルオキシレドキシンはマダニの吸血および産卵時の抗酸化機構に関与する

田仲哲也, 草木迫浩大, 正谷達謄, 宮田健, Galay Remil Linggatong, 白藤梨可, 宮, 前田大輝, Talactac Melbourne Rio, 辻尚利, 望月雅美, 藤崎幸蔵

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　88回・38回　[4T10L-07(3P0466)]　2015/12
Publisher: (公社)日本生化学会
ベクターマダニ体内におけるBabesia ovata増殖動態についての一考察

前田大輝, 前田大輝, 八田岳士, 松林誠, 三好猛晴, 白藤(梅宮, 梨可, 大久保和洋, 五十嵐郁男, 辻尚利, 望月雅美, 望月雅美, 藤崎幸蔵, 田仲哲也, 田仲哲也

日本獣医学会学術集会講演要旨集　158th　302-302　2015/08/30
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニにおけるペルオキシレドキシンの役割について

草木迫浩大, GALAY Remil Linggatong, 白藤(梅宮)梨可, 前田大輝, TALACTAC Melbourne Rio, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　158th　301-301　2015/08/30
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
ダニ媒介性脳炎ウイルスの代替としてのLangatウイルスに対するHaemaphysalis longicornisロンギシンP4ペプチドの殺ウイルス活性(Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus)

Talactac Melbourne Rio, 好井健太朗, 辻尚利, 藤崎幸蔵, 田仲哲也, 望月雅美

日本獣医学会学術集会講演要旨集　158回　345-345　2015/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニ由来2-Cys型ペルオキシレドキシン(HIPrx2)の抗酸化部位の検索

草木迫浩大, 正谷達謄, 宮田健, GALAY Remil Linggatong, 前田大輝, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本寄生虫学会大会プログラム・抄録集　84th　2015

ISSN： 1348-4613
フタトゲチマダニ(Haemaphysalis longicornis)におけるフェリチン類の重要な機能とダニ駆除の標的分子としての可能性(The critical functions of ferritins in Haemaphysalis longicornis and their potential as target molecules for tick control)

Galay Remil Linggatong, 白藤梨可, 宮, 宮田健, 草木迫浩大, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　157回　238-238　2014/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
マウス寄生性コクシジウムEimeria krijgsmanniの生活環に関する形態学的観察

小野結菜, 松林誠, 川口博明, 辻尾祐志, 田仲哲也, 三好宣彰, 松井利博, 松尾智英

日本獣医学会学術集会講演要旨集　157回　355-355　2014/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニ脂肪体の卵黄タンパク質前駆体合成におけるtarget of rapamycin(TOR)の役割

白藤(梅宮)梨可, DAMDMSUREN Boldbaatar, MIN Liao, BANZRAGCH Battur, MORSHEDUR Rahman Md, THASANEEYA Kuboki, LINGGATONG, Galay Remil, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　83rd　79　2014/02

ISSN： 1348-4613
フタトゲチマダニ由来2-Cys型ペルオキシレドキシンの機能解析

草木迫浩大, 草木迫浩大, 正谷達謄, GALAY Remil Linggatong, GALAY Remil Linggatong, 前田大輝, 前田大輝, 望月雅美, 望月雅美, 藤崎幸蔵, 田仲哲也, 田仲哲也

日本獣医学会学術集会講演要旨集　157th　2014

ISSN： 1347-8621
フタトゲチマダニ由来ロイシンリッチリピートドメイン保有蛋白質の動態と機能解析

栗巣孔士, 前田大輝, REMIL Linggatong Galay, 武智理恵, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　65　(2)　2014

ISSN： 0424-7086
フタトゲチマダニ由来TNF Receptor Associated Factor(TRAF)の同定とその役割について

武智理恵, GALAY Remil Linggatong, 前田大輝, 草木迫浩大, 松尾智英, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　157th　2014

ISSN： 1347-8621
フタトゲチマダニ由来TNF Receptor Associated Factorの同定とその役割について

武智理恵, REMIL Linggatong Galay, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　65　(2)　2014

ISSN： 0424-7086
ラクトフェリンによる抗マラリア原虫機能の解析

小森園亮, 柿本麻美子, 田仲哲也, LI Changchun, 渡部久実, HISHAM Ibrahim, 若林裕之, 山内恒治, 宮田健

日本フードファクター学会学術集会講演要旨集　19th　112　2014
フタトゲチマダニ体内におけるB.microtiの検出

草木迫浩大, 前田大輝, GALAY Remil Linggatong, 松尾智英, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　156th　236-236　2013/08/30
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニ由来C型レクチン様蛋白質の同定とその特性解明

前田大輝, 草木迫浩大, GALAY Remil Linggatong, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　156th　235-235　2013/08/30
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニHaemaphysalis longicornisにおいてフェリチンは鉄介在性酸化ストレスを防御する(Ferritins protect the hard tick Haemaphysalis longicornis from iron-mediated oxidative stress)

Galay Remil Linggatong, 草木迫浩大, 前田大輝, 白藤梨可, 梅宮, Tan Bacolod Eugene, 小山次郎, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　156回　235-235　2013/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニロンギシンにおける抗バベシア活性を持つペプチド部位の検索

田仲哲也, GALAY Remil Linggatong, 前田大輝, MIN Aung Kyaw, 白藤(梅宮)梨可, 玄学南, 五十嵐郁男, 望月雅美, 辻尚利, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　155th　217-217　2013/03/04
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
マダニの卵黄タンパク質前駆体合成を制御する分子機構の解明

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, LIAO Min, BATTUR Banzragch, RAHMAN Morshedur Md, KUBOKI Thasaneeya, GALAY Remil Linggatong, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　155th　82-82　2013/03/04
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニにおけるウサギ血液由来トランスフェリンの動態.

森寛行, GALAY R. L., 前田大輝, 松尾智英, 白藤(梅宮)梨可, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　64　(2)　2013

ISSN： 0424-7086
フタトゲチマダニの鉄依存性酸化ストレスから保護するフェリチンの重要性

田仲哲也, GALAY Remil L, 白藤(梅宮)梨可, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵

日本分子生物学会年会プログラム・要旨集(Web)　36th　2P-0295 (WEB ONLY)　2013
マダニのオートファジー関連遺伝子とその役割

白藤梨可, 梅宮, 川野優, D, ボルドバータル, 松尾智英, 田仲哲也, 藤崎幸蔵

獣医寄生虫学会誌　11　(1)　25-25　2012/10
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニにおける宿主血液由来トランスフェリンの動態について

田仲哲也, 森寛行, R.L.・ガレイ, 前田大輝, 白藤梨可, 宮, 藤崎幸蔵

獣医寄生虫学会誌　11　(1)　52-52　2012/10
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニ由来サイクロフィリンAとバベシア原虫の関連性

前田大輝, DAMDINSUREN Boldbaatar, 草木迫浩大, KYAW MIN Aung, GALAY Remil, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　154th　216-216　2012/08/31
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニ卵黄タンパク質前駆体合成におけるTOR阻害剤ラパマイシンの影響

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　154th　204-204　2012/08/31
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
カタダニHaemaphysalis longicornisの吸血と生殖の遂行における鉄蓄積タンパク質であるフェリチンの重要性(The importance of iron storage protein ferritin on successfu blood feeding and reproduction of the hard tick Haemaphysalis longicornis)

Galay Remil, Aung Kyaw Min, 白藤梨可, 宮, 前田大輝, 松尾智英, 川口博明, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　154回　203-203　2012/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニ由来サイクロフィリンAの抗バベシア原虫作用

前田大輝, 田仲哲也, BOLDBAATAR D, 草木迫浩大, AUNG KM, GALAY RL, 白藤(梅宮)梨可, 藤崎幸蔵

衛生動物　63　(2)　166-166　2012/06/30
Publisher: 日本衛生動物学会
ISSN： 0424-7086
微生物貪食におけるフタトゲチマダニクラスBスカベンジャー受容体の重要性

キャウ・ミン・アウン, ダミディスレン・ボルドバータル, 白藤梨可, 宮, ガライ・レミル・リンガートン, 久米愛子, 田仲哲也, 藤崎幸蔵

衛生動物　63　(2)　166-166　2012/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086
フタトゲチマダニにおける宿主血液由来トランスフェリンの動態について

田仲哲也, 森寛行, GALAY Remil Linggatong, 前田大輝, 白藤(梅宮)梨可, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　153rd　225-225　2012/03/01
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
マダニのオートファジー関連遺伝子とその役割

白藤(梅宮)梨可, 川野優, BOLDBAATAR Damdinsuren, 松尾智英, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　153rd　111-111　2012/03/01
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニの吸血および産卵におけるフェリチンの重要性

GALAY Remil L, KYAW MIN Aung, 白藤(梅宮)梨可, 前田大輝, 松尾智英, 川口博明, 三好宣彰, 鈴木宏志, XUAN Xuenan, 望月雅美, 藤崎幸蔵, 田仲哲也

日本分子生物学会年会プログラム・要旨集(Web)　35th　2P-0080 (WEB ONLY)　2012
フタトゲチマダニにおけるオートファジー関連遺伝子HlATG6発現抑制の産卵への影響

川野優, 白藤(梅宮)梨可, BOLDOBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　62　(2)　158-158　2011/06/15
Publisher: 日本衛生動物学会
ISSN： 0424-7086
フタトゲチマダニにおけるセリン/スレオニンキナーゼAktの発現及び機能解析

田中徹, 白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　62　(2)　158-158　2011/06/15
Publisher: 日本衛生動物学会
ISSN： 0424-7086
TOR経路はフタトゲチマダニのビテロジェニン合成に必須である.

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　62　(2)　157-157　2011/06/15
Publisher: 日本衛生動物学会
ISSN： 0424-7086
フタトゲチマダニにおけるクラスBスカベンジャー受容体CD36の機能解析

Aung Kyaw Min, Boldobaatar Damdinsuren, 白藤梨可, 宮, 田仲哲也, 藤崎幸蔵

衛生動物　62　(2)　158-158　2011/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086
フタトゲチマダニロンギシンのトキソプラズマ原虫に及ぼす影響について

田仲哲也, 前田大輝, 松尾智英, DAMDINSUREN Boldbaatar, 白藤(梅宮)梨可, 玄学南, 辻尚利, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　151st　214-214　2011/03/01
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
マダニにおけるオートファジーの役割

白藤(梅宮)梨可, 松尾智英, 川野優, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　151st　107-107　2011/03/01
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニのビテロジェニン合成におけるTOR経路の役割

白藤梨可, 梅宮, ボルドバータル D, 田仲哲也, 藤崎幸蔵

獣医寄生虫学会誌　9　(2)　169-169　2011/03
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニ由来クラスBスカベンジャー受容体CD36の同定とその特性(Identification and characterization of class B scavenger receptor CD 36 from the hard tick, Haemaphysalis longicornis)

アウン K.M, ボルドバータル D, 寥敏, 白藤梨可, 宮, 中尾純大, 松岡輝重, 田仲哲也, 藤崎幸蔵

獣医寄生虫学会誌　9　(2)　170-170　2011/03
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニにおけるオートファジー関連遺伝子H1ATG6の機能解析

川野優, 白藤梨可, 宮, ボルドバータル D, 田仲哲也, 藤崎幸蔵

獣医寄生虫学会誌　9　(2)　171-171　2011/03
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
elF4E遺伝子の機能解明;フタトゲチマダニの生殖系細胞の発育に必須であると思われる

高澤木綿子, BOLDBAATAR Damdinsuren, 久米愛子, 白藤(梅宮)梨可, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　80th　84　2011/02

ISSN： 1348-4613
フタトゲチマダニの翻訳抑制遺伝子HI4EBPの特性解明

久米愛子, BOLDBAATAR Damdinsuren, 高澤木綿子, 白藤(梅宮)梨可, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　80th　84　2011/02

ISSN： 1348-4613
フタトゲチマダニロンギシンP4のトキソプラズマに対する抗原虫作用

田仲哲也, 松尾智英, MORSHEDUR Rahman Md, BOLDBAATAR Damdinsuren, 白藤(梅宮)梨可, XUAN Xuenan, 辻尚利, 藤崎幸蔵

生化学　83回・33回　ROMBUNNO.4P-1168-1168　2010/12
Publisher: (公社)日本生化学会
ISSN： 0037-1017
フタトゲチマダニHaemaphysalis longicornis由来のクラスBスカベンジャー受容体CD36の同定と性状解析(Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis)

Aung Kyaw Min, Boldbaatar Damdinsuren, Liao Min, 白藤梨可, 宮, 中尾純大, 松岡輝重, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　150回　192-192　2010/09
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニのビテロジェニン合成におけるTOR経路の役割

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　150th　192-192　2010/09/01
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
ヒトデフェンシンのトキソプラズマに対する抗原虫作用

田仲哲也, M.・ラハマン, B, バッドゥル, D, ボルドバータル, 玄学南, 藤崎幸蔵

獣医寄生虫学会誌　9　(1)　66-66　2010/09
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニForkhead転写因子の同定と特性解明(Identification and characterization of Forkhead transcription factor from the hard tick, Haemaphysalis longicornis)

D.・ボルドバータル, 白藤梨可, 宮, 田仲哲也, 松岡輝重, 藤崎幸蔵

獣医寄生虫学会誌　9　(1)　72-72　2010/09
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
2 Expression Kinetics of Lysozyme on Haemaphysalis longicornis(Proceedings of the 59th Annual Meeting of Southern Region)

Tanaka T, Kawano S, Nakao S, Shirafuji-Umemiya R, Rahman Md. Morshedur, Battur Banzragch, Boldbaata Damdinsuren, Liao M, Fujisaki K

Medical entomology and zoology　61　(2)　181-181　2010/06/15
Publisher: 日本衛生動物学会
ISSN： 0424-7086
3 Tick Cathepsin B-like Protease Involved in Embryonic Degradation of Vitellin(Proceedings of the 59th Annual Meeting of Southern Region)

Boldbaatar Damdinsuren, Battur Banzragch, Shirafuji-Umemiya R, Liao M, Rahman Md. Morshedur, Tanaka T, Fujisaki K

Medical entomology and zoology　61　(2)　181-181　2010/06/15
Publisher: 日本衛生動物学会
ISSN： 0424-7086
マダニカテプシンB様プロテアーゼはビテリンの胎生期分解と関係がある(Tick Cathepsin B-like Protease Involved in Embryonic Degradation of Vitellin)

Boldbaatar Damdinsuren, Battur Banzragch, 白藤梨可, 梅宮, 廖敏, Rahman, Md. Morshedur, 田仲哲也, 藤崎幸蔵

衛生動物　61　(2)　181-181　2010/06
Publisher: 日本衛生動物学会
ISSN： 0424-7086
フタトゲチマダニにおけるセリン/スレオニンキナーゼAktの発現解析

田中徹, 白藤(梅宮)梨可, DAMDINSUREN Boldbaatar, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　79th　66　2010/05

ISSN： 1348-4613
マダニ類(フタトゲチマダニ)由来のForkhead転写因子の同定と性状解析(Identification and characterization of Forkhead transcription factor from the hard tick, Haemaphysalis longicornis)

Damdinsuren Boldbaatar, 白藤梨可, 宮, 田仲哲也, 松岡輝重, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　149回　242-242　2010/03
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
マダニの遺伝子活性化におけるGATAタンパクの翻訳:フタトゲチマダニのGATA転写因子の同定

BOLDBAATAR Damdinsuren, BATTUR Banzragch, 白藤(梅宮)梨可, LIAO Min, 田仲哲也, 藤崎幸蔵

日本ダニ学会誌　18　(1)　39-40　2009/05/25

ISSN： 0918-1067
フタトゲチマダニのディフェンシン様ペプチド(ロンギシン)の構造とその細胞溶解活性

RAHMAN Md.Morshedur, BOLDBAATAR Damdinsuren, LIAO Min, 白藤(梅宮)梨可, 辻尚利, 田仲哲也, 田原口智士, 藤崎幸蔵

日本ダニ学会誌　18　(1)　39　2009/05/25

ISSN： 0918-1067
フタトゲチマダニにおけるリゾチームの性状解析

田仲哲也, 川野優, 中尾純大, 白藤(梅宮)梨可, MD.MORSHEDUR Rahman, BOLDBAATAR Damdinsuren, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　78th　89　2009/02

ISSN： 1348-4613
フタトゲチマダニのオートファジー関連遺伝子の発現解析

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, LIAO Min, MD.MORSHEDUR Rahman, BATTUR Banzragch, 松尾智英, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　78th　86　2009/02

ISSN： 1348-4613
Identification and characterization of FK506-binding protein from hard tick Haemaphysalis longicornis

Boldbaatar Damdinsuren, Kilonzo Robert Musyoka, Battur Banzragch, Umemiya R, Liao Min, Tanaka T, Xuan X, Fujisaki K

Journal of the Acarological Society of Japan　17　(1)　41-41　2008/05/25
Publisher: 日本ダニ学会
ISSN： 0918-1067
Cloning of autophagy-related genes from a hard tick, Haemaphysalis longicornis and their expression at the unfed stage

Umemiya R, Matsuo T, Hatta T, Boldbaatar Damdinsuren, Tanaka T, Fujisaki K

Journal of the Acarological Society of Japan　17　(1)　41-42　2008/05/25
Publisher: 日本ダニ学会
ISSN： 0918-1067
フタトゲチマダニのオートファジー関連遺伝子の単離・同定と中腸細胞におけるオートファゴソーム様構造体の観察

白藤(梅宮)梨可, 松尾智英, BOLDBAATAR Damdinsuren, LIAO Min, 田仲哲也, 藤崎幸蔵

日本寄生虫学会大会プログラム・抄録集　77th　41　2008/03

ISSN： 1348-4613
フタトゲチマダニ由来の多発性ビトロジェニン遺伝子の同定とそのmRNA発現動態

Boldbaatar Damdinsuren, Battur Banzragch, 梅宮梨可, 廖敏, 田仲哲也, 玄学南, 藤崎幸蔵

獣医寄生虫学会誌　6　(2)　115-115　2008/03
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
Protein Disulfide Isomerases RNA干渉によるマダニHeamaphysalis longicornisの吸血と産卵に及ぼす影響

廖敏, 梅宮梨可, コン海燕, Harnnoi Thasaneeya, Boldbaatar Damdinsuren, 田仲哲也, 玄学南, 藤崎幸蔵

獣医寄生虫学会誌　6　(2)　116-116　2008/03
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニにおけるオートファジー関連遺伝子の発現動態と未吸血成ダニ中腸細胞における局在

梅宮梨可, 松尾智英, 八田岳士, D, ・ボルドバータル, 田仲哲也, 藤崎幸蔵

獣医寄生虫学会誌　6　(2)　123-123　2008/03
Publisher: 日本獣医寄生虫学会
ISSN： 1347-961X
フタトゲチマダニ由来の多発性ビテロジェニン遺伝子の同定とそのmRNA発現動態

DAMDINSUREN Boldbaatar, 梅宮梨可, BANZRAGCH Battur, 田仲哲也, 玄学南, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　144th　67-67　2007/08/27
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
フタトゲチマダニにおけるオートファジー関連遺伝子の発現動態と未吸血成ダニ中腸細胞における局在

梅宮梨可, 松尾智英, 八田岳士, DAMDINSUREN Boldbaatar, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　144th　69-69　2007/08/27
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
Comparison of activity and stability between native and recombinant bovine lactoperoxidase

S. Sato, T. Tanaka, K. Nakao, H. Kumura, K. Shimazaki

Australian Journal of Dairy Technology　58　209　2003/08/01

ISSN： 0004-9433
トキソプラズマ感染防御におけるIFNγの役割

長沢秀行, 鎌田剛徳, 西川義文, 田仲哲也, 井上昇, 藤崎幸蔵, 見上彪, 鈴木直義

原生動物学雑誌　33　(1)　70　2000/03/21

ISSN： 0388-3752
バキュロウイルスによるB.equiEMA-2の発現及び診断用抗原としての応用

田仲哲也, 玄学南, 筏井宏実, 五十嵐郁男, 長澤秀行, 藤崎幸蔵, 見上彪, 鈴木直義

日本獣医学会学術集会講演要旨集　128回　166-166　1999/09
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
バキュロウイルスによるB.equi EMA-1の発現と診断用抗原としての応用

玄学南, 五十嵐郁男, 筏井宏実, Avarzed A, 田仲哲也, 井上昇, 長澤秀行, 藤崎幸蔵, 豊田裕, 鈴木直義

日本獣医学会学術集会講演要旨集　126回　191-191　1998/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
Structural and Immunochemical Studies of Bovine Anitmicrobial Peptide 'lactoferricin'

SHIMAZAKI Kei-ichi, NAM Myoung Soo, HARAKAWA Shinji, TANAKA Tetsuya, OMATA Yoshitaka, SAITO Atsushi, KUMURA Haruto, MIKAWA Katsuhiko, IGARASHI Ikuo, SUZUKI Naoyoshi

1996　(0)　197-200　1996/10/01
抗ラクトフェリンモノクローナル抗体の結合する部位の推定

島崎敬一, 神尾真, NAM Myoung Soo, 玖村朗人, 三河勝彦, 原川信二, 田仲哲也, 小俣吉孝, 齋藤篤志, 五十嵐郁夫, 鈴木直義

日本分子生物学会年会プログラム・講演要旨集　19　(0)　399-399　1996/08/01
ウシラクトフェリンに対するモノクローナル抗体について : 食品

神尾真, 南明〓, 島崎敬一, 玖村朗人, 三河勝彦, 原川信二, 田仲哲也, 小俣吉孝, 斎藤篤志, 五十嵐郁男, 鈴木直義

日本農藝化學會誌　70　(0)　2-2　1996/03/05
Publisher: 社団法人日本農芸化学会
ISSN： 0002-1407
抗ラクトフェリシンモノクローナル抗体の作製とその性状 : 生体高分子・脂質

南明洙, 三河勝彦, 島崎敬一, 原川信二, 田仲哲也, 小俣吉孝, 斎藤篤志, 五十嵐郁男, 鈴木直義

日本農藝化學會誌　69　(0)　180-180　1995/07/05
Publisher: 社団法人日本農芸化学会
ISSN： 0002-1407
抗ラクトフェリシン‐モノクローナル抗体の作成

原川信二, 田仲哲也, 小俣吉孝, 島崎敬一, 五十嵐郁男, 斎藤篤志, 鈴木直義

日本農芸化学会北海道支部・北海道農芸化学協会シンポジウム及び合同学術講演会講演要旨　1994　30　1994

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Books and Other Publications 12

犬のライム病（動物の感染症<第五版>）

田仲哲也, 編集:迫田義博、秋庭正人、末吉益雄、高野友美、長井誠、芳賀猛、村瀬敏之

2025
Ⅳ節足動物 1. ダニ類（動物寄生虫病学<四訂版>）

田仲哲也, 板垣匡, 藤﨑幸蔵

2019
犬のライム病（動物の感染症<第四版>）

田仲哲也, 編集, 明石博臣, 内田郁夫, 大橋和彦, 後藤義孝, 須永藤子, 高井伸二, 宝達勉

2019
"Ticks’ Antioxidant Complex: A Defense Stronghold and a Potential Target for Their Control" in Advances in Medicine and Biology Volume 116

Galay, R. L, Hernandez, E. P, Talactac, M. R, Kusakisako, K, Fujisaki, K, TANAKA Tetsuya, Editors, Leon V. Berhar

2017
"RNA Interference: A Powerful Functional Analysis Tool for Studying Tick Biology Towards Tick and Pathogen Control" in RNA Interference

Galay, R. L, Umemiya-Shirafuji, R, Mochizuki, M, Fujisaki, K, TANAKA Tetsuya (Editors, Ibrokhim Y. Abdurakhmonov

2016
"Host immunization with recombinant proteins to screen antigens for tick control" in Vaccine Design: Methods and Protocols

Galay, R. L, Miyata, T, Umemiya-Shirafuji, R, Mochizuki, M, Fujisaki, K, TANAKA Tetsuya (Editors, Sunil Thomas

2016
第2章免疫の概念（獣医学教育モデル・コア・カリキュラム準拠獣医免疫学）

田仲哲也, 池田輝雄, 岡本まり子, 松本安喜, 編集, 池田輝雄, 小川健司, 松本安喜

2015
第6章節足動物各論I（ダニ）（獣医学教育モデル・コア・カリキュラム準拠寄生虫病学）

田仲哲也, 編集, 堀井洋一郎, 野中成晃, 加藤大智, 平恵介, 松本淳, 山直明

2014
"Tick lysozymes" in Microbial pathogens and strategies for combating them: science, technology and education

TANAKA Tetsuya, Fujisaki, K. (Editors, A. Mendez-Vilas

Formatex Research Center. Spain　2013
"Expression and characterization of bovine milk antimicrobial proteins lactoperoxidase and lactoferrin by vaccinia virus" in Infectious Diseases in Grobal Scenario

TANAKA Tetsuya, Xuan X, Fujisaki, K, Shimazaki, K. (Editors, Priti Kumar Ro

InTech-Open Access Publisher. Croatia　2012
"Antimicrobial activity of lactoferrin and lactoperoxidase in milk" in Dietary Protein Research Trends

TANAKA Tetsuya, Editors, Janet R. Ling

Nova Science Publishers, Inc. New York　2007
3章牛乳とその成分の分析（乳肉卵の機能と利用）

島崎敬一, 田仲哲也, 編集, 阿久澤良造, 坂田亮一, 島崎敬一, 服部昭仁

（株）アイ・ケイコーポレーション　2005

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Presentations 90

フタトゲチマダニ由来抗酸化蛋白質を発現する哺乳類細胞の発現安定株の作製

諸熊遥樹, 草木迫浩大, Talactac Melbourne R, Hernandez Emmanuel P, 田仲哲也

衛生動物　2019/06
単為生殖系フタトゲチマダニの胚発生における抗酸化分子の発現プロファイルの作成

島崎慧, Hernandez Emmanuel Pacia, 新原博子, 藤崎幸蔵, 田仲哲也

衛生動物　2019/06
Haemaphysalis longicornisグルタチオンSトランスフェラーゼ(HlGST2)はダニ幼生におけるクロルピリホス解毒に不可欠である(Haemaphysalis longicornis glutathione S-transferase(HlGST2) is vital in the detoxification of chlorpyrifos in larval ticks)

Hernandez Emmanuel, 草木迫浩大, Talactac Melbourne, Galay Remil, 八田岳士, 藤崎幸蔵, 辻尚利, 田仲哲也

日本獣医学会学術集会講演要旨集　2018/08
哺乳類細胞におけるマダニアクチン遺伝子プロモーターを用いた外来遺伝子の発現

諸熊遥樹, 草木迫浩大, Talactac Melbourne R, Hernandez Emmanuel P, 田仲哲也

衛生動物　2018/06
マダニ胚由来細胞における遺伝子発現ベクターの探索並びに構築

田仲哲也, 井戸明子, 草木迫浩大, 諸熊遥樹, 正谷達謄, Hernandez Emmanuel, Talactac Melbourne, 好井健太朗

生命科学系学会合同年次大会　2017/12
フタトゲチマダニHaemaphysalis longicornisにおいて新規同定されたディフェンシン様ペプチドHEdefensinの性状解析と抗ウイルス活性(Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis)

Talactac Melbourne, 屋田友里花, 好井健太朗, Hernandez Emmanuel, 草木迫浩大, 前田大輝, 藤崎幸蔵, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　2017/08
シカダニIxodes scapularis胚由来細胞株(ISE6)におけるFerritin 1発現誘導(Induction of Ferritin1 expression in Ixodes scapularis embryo derived cell line(ISE6))

Hernandez Emmanuel, 草木迫浩大, Galay Remil, Talactac Melbourne, 好井健太朗, 田仲哲也

日本獣医学会学術集会講演要旨集　2017/08
マダニ-バベシア実験感染モデルの確立

前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林誠, 三好猛晴, 白藤梨可, 宮, 河津信一郎, 五十嵐郁男, 望月雅美, 辻尚利, 田仲哲也

日本獣医学会学術集会講演要旨集　2017/08
マダニペルオキシレドキシンの特性解明(ペルオキシレドキシンの性状解析並びに吸血・産卵に果たす役割について)

草木迫浩大, 正谷達謄, 宮田健, Galay Remil, 白藤梨可, 宮, 前田大輝, Talactac Melbourne, Hernandez Emmanuel, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2017/08
フタトゲチマダニヘモリンフ由来ディフェンシンの同定とその抗菌作用

屋田友里花, 草木迫浩大, Talactac Melbourne R, Hernandez Emmanuel P, 前田大輝, 安藤匡子, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　2017/07
マダニ胚由来細胞における遺伝子発現ベクターの検証

井戸明子, 正谷達謄, 好井健太朗, Galay Remil L, 草木迫浩大, Talactac Melbourne R, Hernandez Emmanuel P, 望月雅美, 田仲哲也

衛生動物　2017/07
Haemaphysalis longicornisにおけるThogotoウイルス(HI-Kamigamo-25)日本単離株の生存動態(Survival dynamics of Japan isolate Thogoto virus(HI-Kamigamo-25) in Haemaphysalis longicornis)

Talactac Melbourne, 好井健太朗, Hernandez Emmanuel, 草木迫浩大, 前田大輝, 藤崎幸蔵, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　2016/08
マダニISE6細胞を用いた過酸化水素発生の可視化についての検討

草木迫浩大, Talactac Melbourne, Hernandez Emmanuel, 前田大輝, 好井健太朗, 望月雅美, 田仲哲也

日本獣医学会学術集会講演要旨集　2016/08
マダニHaemaphysalis longicornis由来の組換えグルタチオンSトランスフェラーゼの同定と性状解析(Identification and characterization of recombinant glutathione S- transferases from the hard tick Haemaphysalis longicornis)

Hernandez Emmanuel, 草木迫浩大, 前田大輝, Galay Remil, Talactac Melbourne, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2016/08
鹿児島県各地で採取されたマダニにおけるマダニ媒介性原虫の分子疫学的解析

正谷達謄, 林慶, 立野守洋, 遠藤泰之, 松尾智英, 田仲哲也, 御供田睦代, 安藤匡子

日本獣医学会学術集会講演要旨集　2016/08
Babesia ovata原虫におけるマダニ中腸形態へのステージシフト誘導実験

前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林誠, 白藤梨可, 梅宮, 辻尚利, 田仲哲也

日本獣医学会学術集会講演要旨集　2016/08
マウス寄生性コクシジウムEimeria krijgsmanni感染の再活性化

水野真伸, 松林誠, 辻尾祐志, 正谷達謄, 田仲哲也, 松井利博, 松尾智英

日本獣医学会学術集会講演要旨集　2016/08
鹿児島県屋久島のヒゲナガチマダニから分離されたリケッチアHKT-1株の遺伝子解析

安藤匡子, 本田俊郎, 御供田睦代, 藤田博己, 矢野泰弘, 正谷達謄, 田仲哲也, 松尾智英, 遠藤泰之, 安藤秀二, 川端寛樹

衛生動物　2016/04
過酸化水素消去酵素2-Cysペルオキシレドキシンはマダニの吸血および産卵時の抗酸化機構に関与する

田仲哲也, 草木迫浩大, 正谷達謄, 宮田健, Galay Remil Linggatong, 白藤梨可, 宮, 前田大輝, Talactac Melbourne Rio, 辻尚利, 望月雅美, 藤崎幸蔵

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　2015/12
ダニ媒介性脳炎ウイルスの代替としてのLangatウイルスに対するHaemaphysalis longicornisロンギシンP4ペプチドの殺ウイルス活性(Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus)

Talactac Melbourne Rio, 好井健太朗, 辻尚利, 藤崎幸蔵, 田仲哲也, 望月雅美

日本獣医学会学術集会講演要旨集　2015/08
フタトゲチマダニにおけるペルオキシレドキシンの役割について

草木迫浩大, Linggatong, Galay Remil, 白藤梨可, 宮, 前田大輝, Talactac Melbourne Rio, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2015/08
Toxoplasma gondii K-3株のシスト形成能に関する研究

小山田昇平, 正谷達謄, 辻尾祐志, 田仲哲也, 松井利博, 松尾智英

日本獣医学会学術集会講演要旨集　2015/08
ベクターマダニ体内におけるBabesia ovata増殖動態についての一考察

前田大輝, 八田岳士, 松林誠, 三好猛晴, 白藤梨可, 宮, 大久保和洋, 五十嵐郁男, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2015/08
フタトゲチマダニ由来TNF Receptor Associated Factor(TRAF)の同定とその役割について

武智理恵, Galay Remil Linggatong, 前田大輝, 草木迫浩大, 松尾智英, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2014/08
フタトゲチマダニ由来2-Cys型ペルオキシレドキシンの機能解析

草木迫浩大, 正谷達謄, Galay Remil Linggatong, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2014/08
マウス寄生性コクシジウムEimeria krijgsmanniの生活環に関する形態学的観察

小野結菜, 松林誠, 川口博明, 辻尾祐志, 田仲哲也, 三好宣彰, 松井利博, 松尾智英

日本獣医学会学術集会講演要旨集　2014/08
フタトゲチマダニ(Haemaphysalis longicornis)におけるフェリチン類の重要な機能とダニ駆除の標的分子としての可能性(The critical functions of ferritins in Haemaphysalis longicornis and their potential as target molecules for tick control)

Galay Remil Linggatong, 白藤梨可, 宮, 宮田健, 草木迫浩大, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2014/08
フタトゲチマダニ由来TNF Receptor Associated Factorの同定とその役割について

武智理恵, Galay Remil Linggatong, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　2014/06
フタトゲチマダニ由来ロイシンリッチリピートドメイン保有蛋白質の動態と機能解析

栗巣孔士, 前田大輝, Galay Remil Linggatong, 武智理恵, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　2014/06
犬と猫のウイルス病におけるダニ媒介性伝播の実験的考察

森寛行, 田仲哲也, 望月雅美

日本獣医学会学術集会講演要旨集　2013/08
マウス腸管寄生性コクシジウムEimeriakrijgsmanniの薬剤感受性

竹尾俊規, 田仲哲也, 松林誠, 辻尾祐志, 橋本和樹, 辻尚利, 藤崎幸蔵, 松井利博, 松尾智英

日本獣医学会学術集会講演要旨集　2013/08
フタトゲチマダニ体内におけるB.microtiの検出

草木迫浩大, 前田大輝, Galay Remil Linggatong, 松尾智英, 白藤梨可, 宮, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2013/08
フタトゲチマダニ由来C型レクチン様蛋白質の同定とその特性解明

前田大輝, 草木迫浩大, Galay Remil Linggatong, 白藤梨可, 宮, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2013/08
フタトゲチマダニHaemaphysalis longicornisにおいてフェリチンは鉄介在性酸化ストレスを防御する(Ferritins protect the hard tick Haemaphysalis longicornis from iron-mediated oxidative stress)

Galay Remil Linggatong, 草木迫浩大, 前田大輝, 白藤梨可, 梅宮, Tan Bacolod Eugene, 小山次郎, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2013/08
フタトゲチマダニにおけるウサギ血液由来トランスフェリンの動態

森寛行, ガライ・レミル・リングガートン, 前田大輝, 松尾智英, 白藤梨可, 宮, 望月雅美, 藤崎幸蔵, 田仲哲也

衛生動物　2013/06
フタトゲチマダニロンギシンにおける抗バベシア活性を持つペプチド部位の検索

田仲哲也, Galay Remil Linggatong, 前田大輝, Aung Kyaw Min, 白藤梨可, 梅宮, 玄学南, 五十嵐郁男, 望月雅美, 辻尚利, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2013/03
マダニの卵黄タンパク質前駆体合成を制御する分子機構の解明

白藤梨可, 梅宮, Boldbaatar Damdinsuren, Liao Min, Battur Banzragch, Rahman Morshedur Md, Kuboki Thasaneeya, Galay Remil Linggatong, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2013/03
フタトゲチマダニの鉄依存性酸化ストレスから保護するフェリチンの重要性

田仲哲也, GALAY Remil L, 白藤(梅宮)梨可, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵

日本分子生物学会年会プログラム・要旨集(Web)　2013
フタトゲチマダニ卵黄タンパク質前駆体合成におけるTOR阻害剤ラパマイシンの影響

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2012/08/31
フタトゲチマダニ由来サイクロフィリンAとバベシア原虫の関連性

前田大輝, DAMDINSUREN Boldbaatar, 草木迫浩大, KYAW MIN Aung, GALAY Remil, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2012/08/31
カタダニHaemaphysalis longicornisの吸血と生殖の遂行における鉄蓄積タンパク質であるフェリチンの重要性(The importance of iron storage protein ferritin on successfu blood feeding and reproduction of the hard tick Haemaphysalis longicornis)

Galay Remil, Aung Kyaw Min, 白藤梨可, 宮, 前田大輝, 松尾智英, 川口博明, 望月雅美, 藤崎幸蔵, 田仲哲也

日本獣医学会学術集会講演要旨集　2012/08
フタトゲチマダニ由来サイクロフィリンAの抗バベシア原虫作用

前田大輝, 田仲哲也, BOLDBAATAR D, 草木迫浩大, AUNG KM, GALAY RL, 白藤(梅宮)梨可, 藤崎幸蔵

衛生動物　2012/06/30
微生物貪食におけるフタトゲチマダニクラスBスカベンジャー受容体の重要性

キャウ・ミン・アウン, ダミディスレン・ボルドバータル, 白藤梨可, 宮, ガライ・レミル・リンガートン, 久米愛子, 田仲哲也, 藤崎幸蔵

衛生動物　2012/06
フタトゲチマダニにおける宿主血液由来トランスフェリンの動態について

田仲哲也, 森寛行, GALAY Remil Linggatong, 前田大輝, 白藤(梅宮)梨可, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2012/03/01
マダニのオートファジー関連遺伝子とその役割

白藤(梅宮)梨可, 川野優, BOLDBAATAR Damdinsuren, 松尾智英, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2012/03/01
フタトゲチマダニの吸血および産卵におけるフェリチンの重要性

GALAY Remil L, KYAW MIN Aung, 白藤(梅宮)梨可, 前田大輝, 松尾智英, 川口博明, 三好宣彰, 鈴木宏志, XUAN Xuenan, 望月雅美, 藤崎幸蔵, 田仲哲也

日本分子生物学会年会プログラム・要旨集(Web)　2012
フタトゲチマダニにおけるオートファジー関連遺伝子HlATG6発現抑制の産卵への影響

川野優, 白藤(梅宮)梨可, BOLDOBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　2011/06/15
TOR経路はフタトゲチマダニのビテロジェニン合成に必須である.

白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　2011/06/15
フタトゲチマダニにおけるセリン/スレオニンキナーゼAktの発現及び機能解析

田中徹, 白藤(梅宮)梨可, BOLDBAATAR Damdinsuren, 田仲哲也, 藤崎幸蔵

衛生動物　2011/06/15
フタトゲチマダニにおけるクラスBスカベンジャー受容体CD36の機能解析

Aung Kyaw Min, Boldobaatar Damdinsuren, 白藤梨可, 宮, 田仲哲也, 藤崎幸蔵

衛生動物　2011/06
フタトゲチマダニロンギシンのトキソプラズマ原虫に及ぼす影響について

田仲哲也, 前田大輝, 松尾智英, Damdinsuren Boldbaatar, 白藤梨可, 梅宮, 玄学南, 辻尚利, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2011/03
マダニにおけるオートファジーの役割

白藤梨可, 梅宮, 松尾智英, 川野優, Boldbaatar Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2011/03
フタトゲチマダニロンギシンP4のトキソプラズマに対する抗原虫作用

田仲哲也, 松尾智英, Morshedur Rahman Md, Boldbaatar Damdinsuren, 白藤梨可, 梅宮, 玄学南, 辻尚利, 藤崎幸蔵

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　2010/12
フタトゲチマダニにおけるオートファジー関連遺伝子HIATG6の機能解析

川野優, 白藤梨可, 梅宮, Boldbaatar Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2010/09
フタトゲチマダニHaemaphysalis longicornis由来のクラスBスカベンジャー受容体CD36の同定と性状解析(Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis)

Aung Kyaw Min, Boldbaatar Damdinsuren, Liao Min, 白藤梨可, 宮, 中尾純大, 松岡輝重, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2010/09
フタトゲチマダニのビテロジェニン合成におけるTOR経路の役割

白藤梨可, 梅宮, Boldbaatar Damdinsuren, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2010/09
3 Tick Cathepsin B-like Protease Involved in Embryonic Degradation of Vitellin(Proceedings of the 59th Annual Meeting of Southern Region)

Boldbaatar Damdinsuren, Battur Banzragch, Shirafuji-Umemiya R, Liao M, Rahman Md. Morshedur, Tanaka T, Fujisaki K

Medical entomology and zoology　2010/06/15
2 Expression Kinetics of Lysozyme on Haemaphysalis longicornis(Proceedings of the 59th Annual Meeting of Southern Region)

Tanaka T, Kawano S, Nakao S, Shirafuji-Umemiya R, Rahman Md. Morshedur, Battur Banzragch, Boldbaata Damdinsuren, Liao M, Fujisaki K

Medical entomology and zoology　2010/06/15
マダニカテプシンB様プロテアーゼはビテリンの胎生期分解と関係がある(Tick Cathepsin B-like Protease Involved in Embryonic Degradation of Vitellin)

Boldbaatar Damdinsuren, Battur Banzragch, 白藤梨可, 梅宮, 廖敏, Rahman, Md. Morshedur, 田仲哲也, 藤崎幸蔵

衛生動物　2010/06
フタトゲチマダニにおけるリゾチームの発現動態

田仲哲也, 川野優, 中尾純大, 白藤梨可, 梅宮, Rahman, Md. Morshedur, Battur Banzragch, Boldbaata Damdinsuren, 廖敏, 藤崎幸蔵

衛生動物　2010/06
マダニ類(フタトゲチマダニ)由来のForkhead転写因子の同定と性状解析(Identification and characterization of Forkhead transcription factor from the hard tick, Haemaphysalis longicornis)

Damdinsuren Boldbaatar, 白藤梨可, 宮, 田仲哲也, 松岡輝重, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2010/03
ヒトデフェンシンのトキソプラズマに対する抗原虫作用

田仲哲也, Morshedur Rahman Md, Banzragch Battur, Damdinsuren Boldbaatar, 玄学南, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2010/03
Cloning of autophagy-related genes from a hard tick, Haemaphysalis longicornis and their expression at the unfed stage

Umemiya R, Matsuo T, Hatta T, Boldbaatar Damdinsuren, Tanaka T, Fujisaki K

Journal of the Acarological Society of Japan　2008/05/25
Identification and characterization of FK506-binding protein from hard tick Haemaphysalis longicornis

Boldbaatar Damdinsuren, Kilonzo Robert Musyoka, Battur Banzragch, Umemiya R, Liao Min, Tanaka T, Xuan X, Fujisaki K

Journal of the Acarological Society of Japan　2008/05/25
ハウスダストマイトアレルゲン由来ProDerp 3の低アレルゲン性

田仲哲也, Magi Mauro, Vandenbranden Michel, 藤崎幸蔵, Jacquet Alain

日本獣医学会学術集会講演要旨集　2007/08
フタトゲチマダニにおけるオートファジー関連遺伝子の発現動態と未吸血成ダニ中腸細胞における局在

梅宮梨可, 松尾智英, 八田岳士, Damdinsuren Boldbaatar, 田仲哲也, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2007/08
フタトゲチマダニ由来の多発性ビテロジェニン遺伝子の同定とそのmRNA発現動態

Damdinsuren Boldbaatar, 梅宮梨可, Banzragch Battur, 田仲哲也, 玄学南, 藤崎幸蔵

日本獣医学会学術集会講演要旨集　2007/08
牛乳由来オステオポンチンのキモシンに対する感受性

三浦敦史, 玖村朗人, 佐藤恵理子, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2004/03
ラクトフェリンのLactobacillus acidophilusに対する増殖促進効果について

大橋美登里, 金完燮, 田仲哲也, 玖村朗人, 島崎敬一

日本畜産学会大会講演要旨集　2003/09
ラクトペルオキシダーゼのメナジオン誘導細胞死に及ぼす影響

石川稿太郎, 田仲哲也, 玖村朗人, 島崎敬一

日本畜産学会大会講演要旨集　2003/09
プロバイオティクス効果を示す酵母の探索および機能解析

田上結子, 塚原真己, 玖村朗人, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2003/09
ウシラクトフェリンのイヌヘルペスウイルスに対する抗ウイルス作用

田仲哲也, 中谷彰吾, 玄学南, 玖村朗人, 五十嵐郁男, 島崎敬一

日本獣医学会学術集会講演要旨集　2003/03
ラット乳線組織におけるpolymeric immunoglobulin receptor(pIgR)の発現に関する研究

飽田恵介, 玖村朗人, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2003/03
経口投与したウシラクトフェリンのラットにおける体内動態

吉瀬蘭エミリー, 田仲哲也, 松本恵, 玖村朗人, 桑田英文, 知地英征, 島崎敬一

日本栄養・食糧学会大会講演要旨集　2002/06
ラットにおける経口投与したウシラクトフェリン及び同フラグンメントの体内動態

吉瀬蘭エミリー, 田仲哲也, 松本恵, 玖村朗人, 知地英征, 島崎敬一

日本畜産学会大会講演要旨集　2002/03
Trypanosoma bruceiのウシラクトフェリン結合タンパク質の検出

田仲哲也, 阿部雪絵, 井上昇, 玖村朗人, 長澤秀行, 島崎敬一

日本獣医学会学術集会講演要旨集　2002/03
ラット乳線上皮細胞におけるpolymeric immunoglobulin receptor(pIgR)発現の定量的検出

飽田恵介, 玖村朗人, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2001/08
ラットα-カゼインのN末端領域ペプチドの分離

高垣謙太郎, 玖村朗人, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2001/08
乳腺上皮細胞におけるpolymeric immunoglobulin receptor(pIgR)の発現因子に関する研究

田中厚志, 玖村朗人, 青塚雅基, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2001/03
二次元電気泳動及びTOF-MSを用いたラット乳腺組織中のタンパク質の分析及び同定

高垣謙太郎, 玖村朗人, 田仲哲也, 島崎敬一

日本畜産学会大会講演要旨集　2001/03
IFN-γにおけるNeospora caninum増殖抑制効果マウス腹腔マクロファージにおける一酸化窒素依存性経路の役割について

田仲哲也, 長澤秀行, 五十嵐郁男, 藤崎幸蔵, 鈴木直義, 見上彪

日本獣医学会学術集会講演要旨集　2000/03
バキュロウイルスによるB.equiEMA-2の発現及び診断用抗原としての応用

田仲哲也, 玄学南, 筏井宏実, 五十嵐郁男, 長澤秀行, 藤崎幸蔵, 見上彪, 鈴木直義

日本獣医学会学術集会講演要旨集　1999/09
Neospora caninum感染BALB/cマウスにおけるCD4+及びCD8+T細胞の役割(T Cell Responses in SAG-1 Transgenic Mice to T.gondii infection)

浜田岳人, 田仲哲也, 井上昇, 長澤秀行, 藤崎幸蔵, 見上彪, 鈴木直義

日本獣医学会学術集会講演要旨集　1999/09
T.gondii主要表面タンパク質P30を発現する組換えネコヘルペスウイルスの作製

玄学南, 三島雅之, 田仲哲也, 西川義文, 五十嵐郁男, 長澤秀行, 藤崎幸蔵, 鈴木直義, 見上彪

日本獣医学会学術集会講演要旨集　1999/03
組換えToxoplasma gondii P24の発現

田仲哲也, 玄学南, 井上昇, 加藤美穂子, 五十嵐郁男, 長澤秀行, 藤崎幸蔵, 見上彪, 鈴木直義

日本獣医学会学術集会講演要旨集　1999/03
バキュロウイルスによるB.equi EMA-1の発現と診断用抗原としての応用

玄学南, 五十嵐郁男, 筏井宏実, Avarzed A, 田仲哲也, 井上昇, 長澤秀行, 藤崎幸蔵, 豊田裕, 鈴木直義

日本獣医学会学術集会講演要旨集　1998/08
抗ラクトフェリンモノクローナル抗体の結合する部位の推定

島崎敬一, 神尾真, NAM Myoung Soo, 玖村朗人, 三河勝彦, 原川信二, 田仲哲也, 小俣吉孝, 齋藤篤志, 五十嵐郁夫, 鈴木直義

日本分子生物学会年会プログラム・講演要旨集　1996/08/01
ウシラクトフェリン並びにウシラクトフェリシン投与マウスにおけるToxoplasma gondii感染に対する防御効果

田仲哲也

日本獣医学会学術集会講演要旨集　1996/04
ウシラクトフェリンに対するモノクローナル抗体について : 食品

神尾真, 南明〓, 島崎敬一, 玖村朗人, 三河勝彦, 原川信二, 田仲哲也, 小俣吉孝, 斎藤篤志, 五十嵐郁男, 鈴木直義

日本農藝化學會誌　1996/03/05
抗ラクトフェリシンモノクローナル抗体の作製とその性状 : 生体高分子・脂質

南明洙, 三河勝彦, 島崎敬一, 原川信二, 田仲哲也, 小俣吉孝, 斎藤篤志, 五十嵐郁男, 鈴木直義

日本農藝化學會誌　1995/07/05

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Industrial Property Rights 4

マラリア原虫増殖阻害用組成物

宮田健, 田仲哲也, 大林桃百香

Property Type: Patent
抗微生物ポリペプチド

田仲哲也, メルボルンリオ, タラクタック, 藤崎幸蔵

Property Type: Patent
抗マダニワクチン

田仲哲也, ガライレミル, リンガートン, 藤崎幸蔵

Property Type: Patent
デキストラン分解酵素

島崎敬一, 玖村朗人, 田仲哲也

Property Type: Patent

Research Projects 27

Analysis of the role of antioxidant molecules in the defense of virus infection using virus-Infected tick

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2025/04/01 - 2029/03/31
RPAとCRISPR/Cas12aを組み合わせたマダニ媒介性感染症の簡易迅速的な診断法の開発

田仲哲也, 一二三達郎

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 挑戦的研究(萌芽)

Institution: 東北大学

2025/06 - 2028/03
東南アジアにおけるマダニ媒介性動物感染症の実態解明と簡易迅速的な診断法の構築

田仲哲也, 山崎渉

Offer Organization: 京都大学東南アジア地域研究研究所

System: 共同利用・共同研究拠点

Category: インキュベーション・プログラム

Institution: 東北大学

2024/04 - 2026/03
多分野融合による節足動物媒介性ウイルスの宿主間伝播・病態形成機構の解明と治療法創出に関する研究開発 Competitive

好井健太朗, 小林純子, 平野港, 田仲哲也, 椎森仁美

Offer Organization: AMED: 国立研究開発法人日本医療研究開発機構

System: 新興・再興感染症研究基盤創生事業（多分野融合研究領域）

Institution: 長崎大学

2023/06 - 2026/03
二本鎖RNAバイオナノカプセルを用いた抗酸化分子を標的とするRNA殺ダニ剤の開発

田仲哲也, 岡村勝友

Offer Organization: 日本学術振興会

System: 科学研究費助成事業基盤研究(B)

Category: 基盤研究(B)

Institution: 鹿児島大学

2022/04/01 - 2025/03/31
二本鎖RNAバイオナノカプセルを用いた抗酸化分子を標的とするRNA殺ダニ剤の開発

田仲哲也, 岡村勝友

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 鹿児島大学

2022/04 - 2025/03

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マダニの生存基盤が宿主動物からの吸血・消化にあり、血液消化産生物中に含まれるヘム代謝の過程で、大量の鉄や過酸化物がマダニ体内に放出される可能性が考えられる。鉄や過酸化物から被る酸化ストレスの制御は、吸血・産卵、また宿主への寄生適応の成否を左右する重要な機構である。研究代表者らはその重要な機構を担う抗酸化分子として、フェリチン(FER)、ペルオキシレドキシン(PRX)、グルタチオンSトランスフェラーゼ(GST)遺伝子を同定した。本研究では、それらを標的とした二本鎖(ds)RNAバイオナノカプセルを用いたRNA殺ダニ剤を開発することを最終目標とする。
Hologenome transcriptomic analysis to underastand the molecular basis of tick-bacteria symbiosis

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Kyushu University

2021/07/09 - 2024/03/31
Genomic epidemiological survey on cattle tick-borne diseases in Philippines and establishment of practical diagnosis

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Institution: Kagoshima University

2020/10/27 - 2024/03/31
Study on the saliva-assisted pathogen transmission mechanism in the tick vector

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

Category: Grant-in-Aid for Scientific Research (B)

Institution: Kitasato University

2020/04/01 - 2024/03/31
新しい遺伝子検出技術を用いたマダニ媒介性動物感染症の簡易・迅速診断方法の開発

田仲哲也

Offer Organization: 科学技術振興機構

System: 産学が連携した研究開発成果の展開研究成果展開事業研究成果最適展開支援プログラム(A-STEP) トライアウトトライアウトタイプ(標準)

Institution: 鹿児島大学

2021 - 2021

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マダニは家畜に甚大な被害を与えているリケッチア症やピロプラズマ症などの多種多様な病原体の伝播に関与し得る、優れた疾病媒介能を有している。このため、マダニとマダニ媒介性動物感染症が世界経済に与える脅威は甚大であり、マダニおよびマダニ媒介性疾病対策は公衆衛生や畜産分野においては不可欠である。このように、マダニ防除のためのワクチン・治療薬開発に繋がる研究に極めて重要な情報をもたらすものとして、マダニ媒介性疾病を公衆衛生学的に評価する疫学的研究が必要とされている。そこで、我々はマダニ媒介性病原体をフィールドでの特殊な整備などを必要としない簡易・迅速的な検出技術を開発することを本研究の目的とした。
Development of tick genetically modified drive to prevent pathogen transmission

TANAKA TETSUYA

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Kagoshima University

2017/06/30 - 2020/03/31

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We attempted to establish a gene knockout technology related to the protection of ticks and aimed to create an infectious disease model using them. To investigate the involvement of defensins in the viral immune response in ticks, we used hemolymph-derived (HE) defensins and examined their effects on virus growth. When the Langat virus was inoculated in the produced HE defensin knockdown / knockout ticks in the body, it had little effect on the survival of the ticks and the virus multiplication in the body. Therefore, it was considered that the antimicrobial peptide is unlikely to be involved in the antiviral immune response to Langat virus in the body of the tick.
Regulation of flavivirus infection by virus-derived long non-coding RNA

YOSHII Kentaro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

2017/04/01 - 2020/03/31

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The 3'-untranslated region (3'-UTR) of tick-borne encephalitis virus (TBEV) plays an important role in the viral pathogenesis. In this study, we investigated detailed mechanisms of virus-derived long non-coding RNA (lncRNA) from the 3'-UTR of TBEV in TBEV infection. Deletions in the 3'-UTR increased virulence in a mouse model and affected the production of virus-derived lncRNA. We identified host proteins interacting the 3'-UTR of TBEV RNA in mammalian and tick cells, and revealed that the interaction affected viral replication mechanisms. These insights are useful for understanding virus transmission and pathogenic mechanism of flavivirus.
Creation of vaccines and RNA acaricides targeting tick antioxidative molecules for controlling ticks

TANAKA TETSUYA

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Kagoshima University

2016/04/01 - 2019/03/31

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The purpose of this study was to clarify the characteristics of the redox balance control mechanism of ticks, to provide new knowledge to the development, research of tick suppression, and to create anti-tick vaccines and RNA acaricides targeting them. Therefore, we worked on the theme of antioxidant molecules, and identified and characterized peroxiredoxin (Prx), glutathione S-transferase (GST), and ferritin (Fer). We investigated the role of antioxidant molecules against acaricides and embryonic development of ticks. Thus, exploiting the properties of antioxidant molecules has been demonstrated to be a promising target molecule for combating ticks.
Epidemiological study of bovine tick-borne diseases and its establishment of diagnosis/preventive methods in Philippins

TANAKA TETSUYA

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

Category: Grant-in-Aid for Scientific Research (B)

Institution: Kagoshima University

2015/04/01 - 2019/03/31

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Bovine tick-borne diseases including anaplasmosis and babesiosis causes great economic losses to animal industry worldwide and designated as an exotic infectious disease. Objective of the present study was to develop new gene diagnostic test against anaplasmosis and babesiosis to do molecular epidemiological survey on anaplasmosis and babesiosis in Philippines, and to contribute for strength of the quarantine system in Japan. As a result, new gene diagnostics was developed against bovine anaplasmosis and babesiosis. The epidemiological surveillance at the surround area of Manila in Philippines demonstrated high prevalence of tick-borne pathogens including anaplasmosis and babesiosis in these regions.
Archiving of the symbionts of arthropods for controlling vector-borne diseases

Nakao Ryo, ABE takashi, TANAKA tetsuya

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)

Category: Grant-in-Aid for Young Scientists (A)

Institution: Hokkaido University

2015/04/01 - 2019/03/31

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Some of the arthropods such as ticks can carry pathogens to humans and animals. The present study was aimed to disclose the diverse microbiome in such vector arthropods. We found that some bacteria such as Coxiella and Rickettsia dominantly exist in ticks. Comparison between tick phylogeny and bacterial genotypes revealed that ticks have acquired the dominant bacteria via both vertical and horizontal transmission.
マダニの低酸素応答機構の解明と抗マダニ薬の創出

辻尚利, 八田岳士, 田仲哲也, 白藤梨可

Offer Organization: 日本学術振興会

System: 科学研究費助成事業基盤研究(A)

Category: 基盤研究(A)

2013/05/31 - 2017/03/31

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これまでに取り組んできた『マダニの吸血と病原体伝播の分子基盤の解明』から、抗マダニ薬を開発する創薬プラットフォームの構築において、創薬分子の標的となる『吸血・病原体伝播調節物質』が低酸素応答によって一括制御されていることを受けていることが判明している。開始３年目の本年度は、これまでに明らかにされたマダニにおける低酸素応答経路と吸血行動を支える代謝系経路の相互作用の知見をもとに、バベシア原虫感染マダニにおける原虫と低酸素応答経路の相互関係の解析を実施した。はじめに、マダニ個体内におけるバベシア原虫の分化・増殖はマダニ代謝に依存することが分かってきていることを受け、低酸素応答機構と原虫生存に与える影響を調べ、細胞内外、臓器間に構築されたネットワークと、病原体媒介の相互関係の動的変化を病原体伝播システムとして捉えた統括的理解を図った。これによって、①原虫感染によるHR調節物質の阻害・制御の動態及びHR調節物質が原虫の発育・伝播に果たす機能を細胞、組織、臓器別に明らかとなった。②各種阻害剤を用いたキナーゼアッセイを行った結果、マダニ個体における原虫伝播時の役割を明らかにすることができた。③原虫感染が解糖系HIF-1、PI-3K/AKT/TOR経路に及ぼす影響を調べた結果、原虫感染による低酸素応答経路に変動があることが分かった。④さらに、分子間クロストークを介した細胞自律的な原虫制御機構としての低酸素応答経路の機能と役割解明から、マダニ媒介性病原体の発育・伝播を支配する分子基盤が存在することが明らかとなった。
Developments for new tick prevantion tageting Toll signal pathway

TANAKA TETSUYA

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

Category: Grant-in-Aid for Scientific Research (B)

Institution: Kagoshima University

2013/04/01 - 2017/03/31

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Toll like receptor (TLR) and tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) are very important to protect againt pathogen infection. Therefore, we alalyzed to idetify and characterize TLR and TRAFD1 from the hard tick Haemaphysalis longicornis. Knockdown of TRAFD1 gene by RNA interference did not affect blood feeding or oviposition. However, TRAFD1 silencing affected the expression of the longicin gene, a defensin-like molecule. On the other hand,recombinant TLR demonstrated growth inhibition activity against B. gibsoni in vitro without a hemolytic effect at used concentration .These results suggested that TLR and TRAFD1 strongly affected innate immunity of ticks.
The preparation of gene knock out tick by TALEN using nuclease for infectious model

TANAKA TETSUYA, MOCHIZUKI MASAMI

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Kagoshima University

2014/04/01 - 2016/03/31

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Although tick studies were conducted by gene silencing using RNA interferance for analyzing the gene function of tick, the skills of gene knockeout and gene transgenic for ticks have not been developing at the morment.Therefore, we tried to incorporate foreign gene into the tick cell line for establishing gene enginering. Although the effect of gene plasmid transfeciton into the tick cell line showed high luceferase activity using pmiGLO or pCAGGS-Luceferase,the effect of plasmid transfection into the tick cell line showed low luceferase activity using actin or ferritin pomotor.These studies indicated that the foreign gene incorporated into the tick may make a break through the praparation of gene konockout or trangenic tick.
マダニの生物活性分子(TBM)を用いた難治性原虫病の予防・治療開発

田仲哲也

System: 産学が連携した研究開発成果の展開研究成果展開事業研究成果最適展開支援プログラム(A-STEP) 探索タイプ

Institution: 鹿児島大学

2012 - 2013

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トキソプラズマ原虫やクリプトスポリジウム原虫はヒトや動物に寄生し、深刻な疾病を引き起こす難治性の原虫病である。我々は難治性原虫病に有効な抗原虫分子を探索していたところ、マダニの自然免疫を担うであるディフェンシン様分子のペプチド部位に抗トキソプラズマ効果を見出した。しかし、このペプチドに対してクリプトスポリジウム原虫に類似したアイメリア原虫のオーシストについては予防効果が認められなかった。今後は当初の目標を達成するために、他のペプチド部位の検索も行い、クリプトスポリジウム原虫とマラリア原虫に対する防御効果についてin vitro、in vivoレベルで解析を行う予定である。
Developments for tick prevention using by baculovirus expressed with high-potential milk protein

TANAKA Tetsuya, FUJISAKI Kozo, XUAN Xuennan

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

Category: Grant-in-Aid for Scientific Research (C)

Institution: Kagoshima University

2010 - 2012

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W e hypothesized that high potential milk protein (HMP) might effect against the hatching and ovipositing of ticks. However, host blood-derived lactoferrin including in the HMP was not detected into tick after blood feeding. When we tried to detect host blood-derived transferrin which has the function aswell as lactoferrin, transferrin showed its unique transfer in tick by passing through the midgut until it reached the ovary. This suggest that host-derived transferrin plays a role in transporting iron to the ovary for embrygenesis.
Developments of transmission-blocking vaccine for tick-borne protozoan diseases

FUJISAKI Kouzo, TANAKA Tetsuya, SUZUKI Hiroshi, TSUJI Naotoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

Category: Grant-in-Aid for Scientific Research (A)

Institution: Kagoshima University

2007 - 2010

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(1) We could identify for the first time vitellogenins (Vg), vitellogeni-receptor (VgR), and longipain as tick-bioactive molecules (TBM) specifically reacted with sexual and sporogonic stages (S-S satages) of Babesia parasites developing in the vector ticks. (2) Since Vg and VgR could inhibit completely the Babesia transmission in ticks treated with their dsRNA and could suppress effectively the Babesia transmission in animals vaccinated with them, the identification of Babesia molecules reacted with them is now concentrated. (3) However, longipain could promote Babesia development in vector ticks after RNAi, it is considered that its application as candidate molecule for the transmission-blocking vaccine may be difficult under the current research situation lacking of the TBM over0expression systems. (4) During the period of this research, we started to focus on TBMs involved in cellular biomass (CB)control in host cells for Babesia parasites because it was noticed that the development of intra-cellular parasites is fundamentally influenced by the essential nutrients supply under the control of CB. (5) Therefore, the molecular identification and characterization of a series of TBMs involving in transcription, translation, post translational modification, and vesicular transport, autophagy (AT) of intra-cellular proteins are concentrated for the first time. (6) These studies are elucidating the significance of TOR pathways (TORs) as candidate molecules for the transmission-blocking vaccine.
マダニの生物活性分子(TBM)の特性とその機能解明 Competitive

System: 共同研究

2007 -
Development and applications of a protozoan parasite vector using Toxoplasma gondii to express heterologous genes

XUAN Xuenan, YOKOYAMA Naoaki, INOUE Noboru, TANAKA Tetsuya, IWATA Akira, TAKASHIMA Yasuhiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

Category: Grant-in-Aid for Scientific Research (B)

Institution: Obihiro University of Agriculture and Veterinary Medicine

2002 - 2005

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An improved method for the construction of recombinant Toxoplasma gondii expressing heterologous genes was developed using pyrimethamine-resistant DHFR-TS and green fluorescent protein (GFP) genes as double selection markers. This improved method allows the construction of recombinant T.gondii expressing foreign genes with great case and speed. Because the foreign genes were placed between the DHFR-TS and GFP genes, all of the drug-resistant and fluorescent clones were confirmed to be expressed foreign genes. This method was employed to construct recombinant T.gondii expressing the Neospora caninum major antigen SRS2 gene (Tg/NcSRS2) and the Cryptosporidium parvum major antigen P23 gene (Tg/CpP23). The expression levels of NcSRS2 and CpP23 in recombinant Tg/NcSRS2 and Tg/CpP23 were similar to those in N.caninum and C.parvum, respectively. The molecular weight and antigenic property of recombinant NcSRS2 and CpP23 were similar to those of native NaSRS2 and CpP23, respectively. Mice immunized with recombinant Tg/NcSRS2 were partially protected from N.caninum challenge infection. On the other hand, the mice immunized with recombinant Tg/CpP23 produced specific neutralizing antibodies against C.parvum. In addition, a bioactive molecule, such as mouse interferon gamma was successfully expressed by T.gondii vector. These results indicate that the T.gondii vector may provide a new tool not only for the production of recombinant vaccines against protozoan parasite infections in animals, but also for the production of bioactive molecules.
原虫特異的アプタマーの作製および診断・治療への応用に関する基礎的研究

井上昇, 杉本千尋, 鈴木宏志, 田仲哲也

Offer Organization: 日本学術振興会

System: 科学研究費助成事業萌芽研究

Category: 萌芽研究

Institution: 帯広畜産大学

2002 - 2003

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1.リパノソーマ増殖に必須であることが報告されているLDLおよびトランスフェリン(TF)に着目し,これらの血漿成分に対するアプタマーが虫体の増殖を阻害するか否かを検討した。予備的に作製した抗牛LDLおよび抗牛TF抗体を用いてIn Vitroでの増殖阻害試験を行った結果,両抗体共に明らかな増殖阻止効果は認められなかった。 2.アプタマー作製用オリゴヌクレオチドを用いた抗トリパノソーマDNAアプタマーの作製においても現時点では期待する増殖阻止効果を示すアプタマーは得られていない。今後さらにペプチドアプタマーなども試験していく必要があると思われる。 3.トリパノソーマ抽出物およびトリパノソーマ虫体に対するDNA/RNAアプタマー作製を試みた。今後考慮・改善すべき点として、DNAまたはRNAアプタマーと細胞由来DNA/RNA結合性蛋白質などとの相互作用によってSELEX反応に予期せぬ選択圧が生じることが考えられる。細胞体あるいは細胞抽出物のような雑多な生体分子の混合物に対するアプタマー作製に当たってはペプチドアプタマーあるいはファージディスプレイなども利用すべきであろう。
Development of Diagnostic Methods for African Trypanosomes and Their Evaluation Using Field Samples

INOUE Noboru, XUAN Xuenan, TANAKA Tetsuya, YOKOYAMA Naoaki, NAGASAWA Hideyuki, FUJISAKI Kozo

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

Category: Grant-in-Aid for Scientific Research (B)

Institution: Obihiro University of Agriculture and Veterinary Medicine

2001 - 2003

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Loop-mediated isothermal amplification of DMA (LAMP) The loop-mediated isothermal amplification (LAMP) reaction is a method that amplifies DNMA with high specificity, efficiency, and rapidity under isothermal conditions with only simple incubators. An added advantage of LAMP over PCR-based methods is that DNA amplification can be monitored spectrophotometrically and/or with the naked eye without the use of dyes. Here we report our conditions for a highly sensitive, specific, and easy diagnostic assay based on LAMP technology for the detection of parasites in the Trypanosoma brucei group (including T.brucei brucei, T.brucei gambiense, T.brucei rhodesiense, and T.evansi) and T.congolense. We show that the sensitivity of the LAMP-based method for detection of trypanosomes in vitro is up to 100 times higher than that of PCR-based methods. In vivo studies in mice infected with human-infective T.brucei gambiense further highlight the potential clinical importance of LAMP as a diagnostic tool for the identification of African trypanosomiasis. ELISA The ability to use mitochondrial heat shock protein 70 (MTP) of Trypanosoma congolense as a diagnostic antigen was examined. The B-cell epitope recognized by MAb 10F9 was located within 206 amino acids from the C terminus. Depending on the conditions of protein extraction, MTP was cleaved into smaller polypeptides by endogenous proteases. However, the C-termmal epitope of MTP was preserved with a high degree of antigenicity, even after cleavage. Antibody detection by enzyme-linked immunosorbent assay with the truncated recombinant MTP revealed that anti-MTP antibodies exist in experimentally infected mouse sera. Thus, MTP may be useful as an antigen for the serodiagnosis of primary T.congolense infection.
ウシラクトフェリンを用いたトキソプラズマ原虫の生体機能の解明

田仲哲也

Offer Organization: 日本学術振興会

System: 科学研究費助成事業若手研究(B)

Category: 若手研究(B)

Institution: 北海道大学

2001 - 2002

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トランスフェリンファミリータンパク質であるラクトフェリン、トランスフェリン、オボトランスフェリンはそれぞれ哺乳類、鳥類などの生体内に分泌される分子量約80,000の鉄結合性タンパク質である。そこで本研究の目的は、トキソプラズマのラクトフェリン、トランスフェリン、オボトランスフェリン結合タンパク質を解析する事によって、原虫の鉄代謝機構を解明することこである。さらに本研究の成果は、抗生物質とは異なった新しい抗原虫病治療薬やワクチンの開発へつながるものと期待できる。そこで、我々はトキソプラズマ原虫のトランスファミリータンパク質に対する結合性タンパク質を解析するために、far Western blot法によって鉄結合性タンパク質とトキソプラズマ原虫の結合性について実験を行った。その結果、ウシラクトフェリン、ウシトランスフェリン、オボトランスフェリンは互いにトキソプラズマ中の同じ可溶性タンパク質に結合すること、その分子量は約42kDaであった(投稿準備中)。また興味深いことに、トランスフェリンレセプターを持つことが知られているトリパノソーマ原虫を用いて同様な実験を行ったところ、ウシラクトフェリン、ウシトランスフェリン、オボトランスフェリンは互いにトリパノソーマ中の同じ分子量約40、43kDa付近の2つのタンパク質と結合することが分った。これらの結果から、同じ原虫であっても、鉄結合性タンパク質に反応するタンパク質は原虫の種類によって異なることが分った。また、40kDaのラクトフェリン結合タンパク質についてN末端アミノ酸配列を決定したところ、グリセルアルデヒド-3-リン酸デヒドロゲナーゼであった。現在43kDaのラクトフェリン結合タンパク質についてはN末端アミノ酸配列を解析しているところである(投稿準備中)。今後はこれらのタンパク質の機能について検討するつもりである。
Structure and functional studies of bovine lactoperoxidase

SHIMAZAKI Kei-ichi, TANAKA Tetsuya, KUMURA Haruto, NAKAMURA Shingo

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B).

Category: Grant-in-Aid for Scientific Research (B).

Institution: HOKKAIDO UNIVERSITY

1999 - 2000

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The cDNA encoding bovine lactoperoxidase has been expressed in CHO cells. The recombinant lactoperoxidase was secreted as an enzymatically active single chain molecule presenting two immunoreactive forms of 88 kDa and 82 kDa, differing by their glycosylation. recombinant lactoperoxidase exhibited the characteristic absorbance spectrum with a Soret peak at 413 nm. Biochemical properties of bovine lactoperoxidase isolated from milk and recombinant bovine lactoperoxidase expressed by CHO cells were compared. The natural and recombinant lactoperoxidases showed the same conformational features as determined by CD measurements. The α-helix, β-structure and unordered structure content was found to be 17.8%, 54.2% and 28.0% for the natural lactoperoxidase and 18.6%, 50.1% and 31.3% for the recombinant lactoperoxidase, respectively. The microenvironments of aromatic amino acid residues in both lactoperoxidases seemed to be the same, although the CD spectral band due to the Soret band differed slightly. A difference in the pH-dependent spectral changes of absorbance at 413 nm was observed. From a pepsin hydrolysate of lactoperoxidase, a heme-binding peptide was isolated by reverse-phase HPLC and its amino acid sequence was examined. Engineering of recombinant lactoperoxidase into a myeloperoxidase-like molecule was attempted by substituting Gln-376 by Met, a residue known to achieve covalent binding with the heme in myeloperoxidase. However, the resulting bovine lactoperoxidase mutant failed to acquire the peculiar absorbance spectrum and the chlorinating activity of myeloperoxidase, underlining the complex nature of interactions in the heme vicinity.

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