研究者詳細

顔写真

アマラシリ カラヘ パンデイサ コララゲ モハン
Amarasiri Kalahe Panditha Koralage Mohan
Amarasiri Kalahe Panditha Koralage Mohan
所属
大学院工学研究科 土木工学専攻 水環境学講座(環境水質工学分野)
職名
准教授
学位
  • 工学博士 (北海道大学)

  • 修士 (北海道大学)

  • 卒業 (ペラデニヤ大学)

e-Rad 研究者番号
50815537

経歴 3

  • 2024年4月 ~ 継続中
    東北大学 大学院工学研究科 准教授

  • 2020年4月 ~ 2024年3月
    北里大学 医療衛生学部 講師

  • 2017年10月 ~ 2020年3月
    東北大学 大学院工学研究科 助教

学歴 3

  • Hokkaido University Division of Environmental Engneering Ph.D.

    2014年10月 ~ 2017年9月

  • Hokkaido University Division of Environmental Engineering M. Eng.

    2012年10月 ~ 2014年9月

  • University of Peradeniya Civil Engineering B Sc Eng

    2007年3月 ~ 2011年9月

委員歴 2

  • 日本水環境学会 JWET委員会

    2023年7月 ~ 継続中

  • International Society for Food and Environmental Virology Conference 2024 Organizing Committee

    2023年3月 ~ 2024年5月

所属学協会 3

  • International Water Association

  • Institute of Engineers, Sri Lanka.

  • 日本水環境学会

研究キーワード 5

  • enteric bacteria

  • antimicrobial resistance

  • Histo-blood group antigens

  • enteric virus

  • Wastewater treatment

研究分野 3

  • ライフサイエンス / 分子生物学 /

  • 環境・農学 / 環境負荷低減技術、保全修復技術 / Antimicrobial resistance

  • 社会基盤(土木・建築・防災) / 土木環境システム / Wastewater treatment

受賞 5

  1. Best Teacher 2021 Award

    2022年3月 Kitasato University, Department of Health Science

  2. Best Paper Award

    2021年4月 Critical Reviews in Environmental Science and Technology

  3. Best Presentation Award

    2019年9月 International Water Association Health Related Water Microbiology Conference

  4. JSWE-Organo doctoral research award

    2017年9月26日 Japanese Society on Water Environment

  5. Excellent Presentation Award

    2013年5月 Japan Society on Water Environment

論文 64

  1. Linking heavy metal(loid) stress to biofilm formation and conjugation: A critical review of experimental evidence

    Katayoun Dadeh Amirfard, Mohan Amarasiri, Daisuke Sano

    Critical Reviews in Environmental Science and Technology 2026年5月6日

    DOI: 10.1080/10643389.2026.2666100  

  2. Emergence of Tigecycline‐Resistant Pseudomonas aeruginosa Harbouring tmexC6D6‐toprJ1b From Hospital Sewage in Japan

    Shotaro Maehana, Masato Suzuki, Naoko Ishimura, Hiroki Izawa, Ryotaro Eda, Masaki Nakamura, Mohan Amarasiri, Takashi Furukawa, Fumiaki Kojima, Kazunari Sei, Makoto Kubo

    Environmental Microbiology Reports 2026年4月

    DOI: 10.1111/1758-2229.70275  

  3. Inactivation of bio-aerosolized viruses by applying pulsed electric field

    Takashi Furukawa, Takahisa Ueno, Suzuha Moroishi, Yuki Hoshiko, Shotaro Maehana, Mohan Amarasiri, Kazunari Sei

    Environmental Pollution 2026年4月

    DOI: 10.1016/j.envpol.2026.127841  

  4. Energy allocation trade-offs among conjugative transfer, biofilm formation, and heavy metal resistance: a dynamic energy budget theory perspective

    Katayoun Dadeh Amirfard, Mohan Amarasiri, Daisuke Sano

    Water Research 2026年3月

    DOI: 10.1016/j.watres.2025.125216  

  5. From wastewater to epidemiological insights: A systematic review of modeling strategies for infectious disease surveillance

    Luyao Wang, Mohan Amarasiri, Wakana Oishi, Daisuke Sano

    Water Research 2026年1月

    DOI: 10.1016/j.watres.2025.124977  

  6. Intranasal phage therapy overcomes antibody neutralization challenges in pulmonary Pseudomonas aeruginosa infections. 国際誌

    Dhammika Leshan Wannigama, Jingping Shao, Hongya Sun, Yangzhong Wang, Cameron Hurst, Peter N Monk, Mohan Amarasiri, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Thin Sandi Htun, Sirirat Luk-In, Yoshitaka Shimotai, Natharin Ngamwongsatit, Hitoshi Ishikawa, Naveen Kumar Devanga Ragupathi, Daniel Pletzer, Talerngsak Kanjanabuch, Aisha Khatib, Kazuhiko Miyanaga, Longzhu Cui, Kenji Shibuya, Paul G Higgins, Anthony Kicic, Parichart Hongsing, Jinxin Zhao, Shuichi Abe, Hiroshi Hamamoto

    Archives of microbiology 207 (12) 310-310 2025年10月18日

    DOI: 10.1007/s00203-025-04526-6  

    詳細を見る 詳細を閉じる

    Phage therapy is a promising approach against multidrug-resistant infections, yet systemic administration can lead to incomplete cures. We investigated the distribution, immune responses, and efficacy of the therapeutic phage KPP10 delivered via intranasal or intraperitoneal (IP) routes in murine Pseudomonas aeruginosa lung infection models. Intranasal pre-treatment achieved markedly higher localization of KPP10 in the lungs and bronchoalveolar compartment compared to IP delivery. Intranasal administration elicited minimal systemic antibody responses, whereas IP injection triggered significant IgG, IgM, and IgA production. Antibody responses did not differ significantly between doses. In acute and chronic infection models, intranasal KPP10 significantly improved survival (p < 0.01) and reduced lung bacterial loads relative to IP injection. Importantly, IP treatment was associated with bacterial rebound after day 14 in chronic infection, whereas intranasal dosing sustained bacterial clearance. These findings demonstrate that intranasal delivery enhances pulmonary localization, minimizes antibody-mediated neutralization, and provides superior therapeutic efficacy, highlighting its potential as a more effective route for phage therapy against P. aeruginosa lung infections.

  7. Streptococcus pyogenes Surveillance Through Surface Swab Samples to Track the Emergence of Streptococcal Toxic Shock Syndrome in Rural Japan

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Yu Suzuki, Kazunori Moriya, Kazuhiko Miyanaga, Longzhu Cui, Angkana T Huang, Yoshikazu Okuma, Daisuke Akaneya, Junko Igarashi, Mayu Suto, Daisuke Ishizawa, Wakana Imamiya, Ayaka Igarashi, Yoshitaka Shimotai, Andrew C Singer, Naveen Kumar Devanga Ragupathi, Takashi Furukawa, Kazunari Sei, Yangzhong Wang, Talerngsak Kanjanabuch, Paul G Higgins, Nobuhito Nemoto, Aisha Khatib, Anthony Kicic, Sam Trowsdale, Parichart Hongsing, Daisuke Sano, Kenji Shibuya, Shuichi Abe, Hiroshi Hamamoto

    The Journal of Infectious Diseases 2025年8月14日

    DOI: 10.1093/infdis/jiaf234  

  8. Usefulness of pulsed electric field application as an inactivation technology for plant pathogenic bacteria in hydroponic nutrient solutions

    Takashi Furukawa, Takahisa Ueno, Masahiro Nozaki, Ayumi Yoshida, Mohan Amarasiri, Kazunari Sei

    Environmental Technology &amp; Innovation 39 104237-104237 2025年8月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.eti.2025.104237  

    ISSN:2352-1864

  9. Global participatory wastewater surveillance to understand Mpox clade diversity in war and conflict-affected countries 査読有り

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Yangzhong Wang, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Nada M Melhem, Andrew C Singer, Naveen Kumar Devanga Ragupathi, Juliana Calabria Araujo, Kazunari Sei, Lazarus G Ndatuwong, Syed Faisal Mahmood, Yoshitaka Shimotai, Talerngsak Kanjanabuch, Paul G Higgins, Tetsuji Aoyagi, Anthony Kicic, Asuka Nanbo, Hirotake Mori, Richard Siow, Özgür Kurt, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Daisuke Sano, Kenji Shibuya, Shuichi Abe, Hiroshi Hamamoto

    Journal of Travel Medicine 2025年6月2日

    出版者・発行元: Oxford University Press (OUP)

    DOI: 10.1093/jtm/taaf052  

    ISSN:1195-1982

    eISSN:1708-8305

    詳細を見る 詳細を閉じる

    Teaser Community-led wastewater surveillance reveals widespread circulation of the mpox virus and clade diversity in conflict-affected countries. This low-cost approach fills critical surveillance gaps, uncovering regional clade variations, including the more severe Clade Ib, and highlighting the potential of decentralized genomic monitoring where traditional systems fail.

  10. Surveillance of avian influenza through bird guano in remote regions of the global south to uncover transmission dynamics 査読有り

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, John Jefferson V. Besa, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T. Huang, Puey Ounjai, W. K. C. P. Werawatte, Ali Hosseini Rad S. M, Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Phitsanuruk Kanthawee, Wanwara Thuptimdang, Ratana Tacharoenmuang, Bernadina Cynthia, S. P. H. Spencer Vitharana, Natharin Ngamwongsatit, Hitoshi Ishikawa, Takashi Furukawa, Yangzhong Wang, Andrew C. Singer, Naveen Kumar Devanga Ragupathi, Tanittha Chatsuwan, Kazunari Sei, Asuka Nanbo, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Hiroshi Hamamoto, Paul G. Higgins, Daisuke Sano, Anthony Kicic, José O. Valdebenito, Jonas Bonnedahl, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe

    Nature Communications 16 (1) 2025年5月27日

    出版者・発行元: Springer Science and Business Media LLC

    DOI: 10.1038/s41467-025-59322-z  

    eISSN:2041-1723

  11. Identifying High-Risk Bacteria with Active Nasal Swab Surveillance in Intensive Care Units to Prevent Ventilator-Associated Pneumonia

    Yu Kuramasu, Yu Suzuki, Daisuke Akaneya, Yoshikazu Okuma, Yuta Tsujimoto, Daisuke Ishizawa, Kazunori Moriya, Parichart Hongsing, Mohan Amarasiri, Cameron Hurst, Paul G. Higgins, Kenji Shibuya, Anthony Kicic, Yoshitaka Shimotai, Hiroshi Hamamoto, Dhammika Leshan Wannigama, Shuichi Abe

    International Journal of Translational Medicine 2025年4月25日

    DOI: 10.3390/ijtm5020017  

  12. tesG expression as a potential clinical biomarker for chronic Pseudomonas aeruginosa pulmonary biofilm infections. 国際誌

    Dhammika Leshan Wannigama, Cameron Hurst, Peter N Monk, Gunter Hartel, William Graham Fox Ditcham, Parichart Hongsing, Phatthranit Phattharapornjaroen, Puey Ounjai, Pattama Torvorapanit, Kamonwan Jutivorakool, Sirirat Luk-In, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Kazuhiko Miyanaga, Longzhu Cui, Naveen Kumar Devanga Ragupathi, S M Ali Hosseini Rad, Aisha Khatib, Robin James Storer, Hitoshi Ishikawa, Mohan Amarasiri, Somrat Charuluxananan, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Jane C Davies, Stephen M Stick, Anthony Kicic, Tanittha Chatsuwan, Kenji Shibuya, Shuichi Abe

    BMC medicine 23 (1) 191-191 2025年3月31日

    DOI: 10.1186/s12916-025-04009-x  

    詳細を見る 詳細を閉じる

    BACKGROUND: Pseudomonas aeruginosa infections in the lungs affect millions of children and adults worldwide. To our knowledge, no clinically validated prognostic biomarkers for chronic pulmonary P. aeruginosa infections exist. Therefore, this study aims to identify potential prognostic markers for chronic P. aeruginosa biofilm lung infections. METHODS: Here, we screened the expression of 11 P. aeruginosa regulatory genes (tesG, algD, lasR, lasA, lasB, pelB, phzF, rhlA, rsmY, rsmZ, and sagS) to identify associations between clinical status and chronic biofilm infection. RESULTS: RNA was extracted from 210 sputum samples from patients (n = 70) with chronic P. aeruginosa lung infections (mean age; 29.3-56.2 years; 33 female). Strong biofilm formation was correlated with prolonged hospital stays (212.2 days vs. 44.4 days) and increased mortality (46.2% (18)). Strong biofilm formation is associated with increased tesG expression (P = 0.001), influencing extended intensive care unit (P = 0.002) or hospitalisation stays (P = 0.001), pneumonia risk (P = 0.006), and mortality (P = 0.001). Notably, tesG expression is linked to the modulation of systemic and sputum inflammatory responses and predicts biofilm biomass. CONCLUSIONS: This study provides the first clinical dataset of tesG expression levels as a predictive biomarker for chronic P. aeruginosa pulmonary infections.

  13. Aptamer-based biosensors for wastewater surveillance of influenza virus, SARS-CoV-2, and norovirus: A comprehensive review

    Yilei Wang, Mohan Amarasiri, Wakana Oishi, Masayasu Kuwahara, Yuka Kataoka, Hiroki Kurita, Fumio Narita, Rong Chen, Qian Li, Daisuke Sano

    Water Research 123484-123484 2025年3月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.watres.2025.123484  

    ISSN:0043-1354

  14. Combination of curcumin or chitosan with photodynamic therapy as an effective alternative therapy for overcoming wound infection associated with multidrug-resistant Acinetobacter baumannii

    Mohan Amarasiri

    MICROBE 2025年

    DOI: 10.1016/J.MICROB.2025.100375  

  15. Tapeworm infection incidence in rural Japan points to a common environmental source of infection

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Yu Suzuki, Daisuke Akaneya, Mika Moriya, Taichi Adachi, Yoshikazu Okuma, Daisuke Ishizawa, Hitoshi Ishikawa, Kazuhiko Miyanaga, Longzhu Cui, Kazunori Moriya, Hirotake Mori, Naveen Kumar Devanga Ragupathi, Yoshitaka Shimotai, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Talerngsak Kanjanabuch, Paul G. Higgins, Tetsuji Aoyagi, Anthony Kicic, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe, Hiroshi Hamamoto

    Drug Discoveries &amp; Therapeutics 2025年

    出版者・発行元: International Research and Cooperation Association for Bio & Socio-Sciences Advancement (IRCA-BSSA)

    DOI: 10.5582/ddt.2025.01070  

    ISSN:1881-7831

    eISSN:1881-784X

  16. Phage cocktail amikacin combination as a potential therapy for bacteremia associated with carbapenemase producing colistin resistant Klebsiella pneumoniae. 国際誌

    Aye Mya Sithu Shein, Dhammika Leshan Wannigama, Cameron Hurst, Peter N Monk, Mohan Amarasiri, Thidathip Wongsurawat, Piroon Jenjaroenpun, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Vishnu Nayak Badavath, Sirirat Luk-In, Sumanee Nilgate, Ubolrat Rirerm, Sukrit Srisakul, Naris Kueakulpattana, Matchima Laowansiri, S M Ali Hosseini Rad, Supaporn Wacharapluesadee, Apaporn Rodpan, Natharin Ngamwongsatit, Arsa Thammahong, Hitoshi Ishikawa, Robin James Storer, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Annika Y Classen, Talerngsak Kanjanabuch, Daniel Pletzer, Kazuhiko Miyanaga, Longzhu Cui, Hiroshi Hamamoto, Paul G Higgins, Anthony Kicic, Tanittha Chatsuwan, Parichart Hongsing, Shuichi Abe

    Scientific reports 14 (1) 28992-28992 2024年11月22日

    DOI: 10.1038/s41598-024-79924-9  

    詳細を見る 詳細を閉じる

    The increasing occurrence of hospital-associated infections, particularly bacteremia, caused by extensively drug-resistant (XDR) carbapenemase-producing colistin-resistant Klebsiella pneumoniae highlights a critical requirement to discover new therapeutic alternatives. Bacteriophages having host-specific bacteriolytic effects are promising alternatives for combating these pathogens. Among 12 phages isolated from public wastewater in Thailand, two phages-vB_kpnM_05 (myovirus) and vB_kpnP_08 (podovirus) showed broad-host range, producing bacteriolytic activities against 81.3% (n = 26) and 78.1% (n = 25) of 32 XDR carbapenemase-producing colistin-resistant K. pneumoniae, with capsular types-K15, K17, K50, K51, K52/wzi-50 and K2/wzi-2. Both phages showed short replication times, large burst sizes with rapid adsorptions. They exhibited significant stability under various environmental conditions. Genomic analysis revealed that both phages are genetically distinct phages from Myoviridae and Podoviridae family, with the lack of toxin, virulence, lysogeny and antibiotic resistance genes. These characteristics highlighted their promising potential for utilizing in phage therapy for combating XDR K. pneumoniae. Although phage cocktail combining vB_kpnM_05 and vB_kpnP_08 provided significant bacteriolysis for longer duration (8 h) than its monophage (6 h), bacterial regrowth was observed which suggested an evitable development of phage resistance under phages' selection pressures. Future study will be undertaken to elucidate the precise mechanisms by which these XDR K. pneumoniae developed phage resistance and their associated fitness cost. Remarkably, combining phage cocktail with amikacin at their sub-inhibitory concentrations produced potent synergy by completely suppressing bacterial regrowth in vitro. Our study demonstrated the significant therapeutic and prophylactic effectiveness of a phage cocktail-amikacin combination as a promising alternative strategy for overcoming bacteremia associated with XDR K. pneumoniae having carbapenemase and colistin resistance in vivo.

  17. Impact of COVID-19 Vaccination in Thailand: Averted Deaths and Severe Infections Across Age Groups

    Chaiwat Wilasang, Pikkanet Suttirat, Dhammika Leshan Wannigama, Mohan Amarasiri, Sudarat Chadsuthi, Charin Modchang

    Tropical Medicine and Infectious Disease 2024年11月22日

    DOI: 10.3390/tropicalmed9120286  

  18. Identification of protein biomarkers in wastewater linked to the incidence of COVID-19 査読有り

    Luhur Akbar Devianto, Mohan Amarasiri, Luyao Wang, Takehito Iizuka, Daisuke Sano

    Science of The Total Environment 951 175649-175649 2024年11月

    DOI: 10.1016/j.scitotenv.2024.175649  

    ISSN:0048-9697

  19. Phage therapy could be key to conquering persistent bacterial lung infections in children. 国際誌

    Aye Mya Sithu Shein, Parichart Hongsing, Aisha Khatib, Phatthranit Phattharapornjaroen, Kazuhiko Miyanaga, Longzhu Cui, Kenji Shibuya, Mohan Amarasiri, Peter N Monk, Anthony Kicic, Tanittha Chatsuwan, Paul G Higgins, Shuichi Abe, Dhammika Leshan Wannigama

    npj antimicrobials and resistance 2 (1) 31-31 2024年10月10日

    DOI: 10.1038/s44259-024-00045-4  

    詳細を見る 詳細を閉じる

    Persistent bacterial lung infections in children lead to significant morbidity and mortality due to antibiotic resistance. In this paper, we describe how phage therapy has shown remarkable efficacy in preclinical and clinical studies, demonstrating significant therapeutic benefits through various administration routes. Ongoing trials are evaluating its safety and effectiveness against different pathogens. Advancing phage therapy through systematic studies and international collaboration could provide a viable alternative to traditional antibiotics for persistent infections.

  20. Degradation of extracellular antibiotic resistance genes using peracetic acid (PAA) and performic acid (PFA) 査読有り

    Mohan Amarasiri, Hiroki Kobayashi, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    Environmental Technology &amp; Innovation 36 103862-103862 2024年10月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.eti.2024.103862  

    ISSN:2352-1864

  21. Community-based mpox and sexually transmitted disease surveillance using discarded condoms in the global south 査読有り

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, John Jefferson V Besa, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Puey Ounjai, W.K.C.P. Werawatte, Ali Hosseini Rad S.M., Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Phitsanuruk Kanthawee, Marily Al-Dois, Rapong Bani, Wanwara Thuptiang, Sifat Khan, Ratana Tacharoenmuang, Bernadina Cynthia, S.P.H Spencer Vitharana, Natharin Ngamwongsatit, Hitoshi Ishikawa, Andrew C Singer, Naveen Kumar Devanga Ragupathi, Takashi Furukawa, Kazunari Sei, Asuka Nanbo, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Tanittha Chatsuwan, Paul G Higgins, Daisuke Sano, Anthony Kicic, Jürgen Kurt Rockstroh, Richard Siow, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe

    The Lancet Infectious Diseases 24 (10) e610-e613 2024年10月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/s1473-3099(24)00514-0  

    ISSN:1473-3099

  22. Cell Extracts Derived from Cypress and Cedar Show Antiviral Activity against Enveloped Viruses 査読有り

    Takashi Furukawa, Ayumu Inagaki, Takeshi Hatta, Suzuha Moroishi, Katsuki Kawanishi, Yuki Itoh, Shotaro Maehana, Mohan Amarasiri, Kazunari Sei

    Microorganisms 12 (9) 1813-1813 2024年9月2日

    出版者・発行元: MDPI AG

    DOI: 10.3390/microorganisms12091813  

    eISSN:2076-2607

    詳細を見る 詳細を閉じる

    The antiviral efficacy of cell-extracts (CEs) derived from cypress (Chamaecyparis obtusa (Siebold &amp; Zucc.) Endl., C. obtusa) and cedar (Cryptomeria japonica (Thunb. ex. L.) D.Don, C. japonica) was assessed using phi6 and MS2 bacteriophages, which are widely accepted surrogate models for enveloped and non-enveloped viruses, in order to verify their potential use as antiviral agents. Our results indicate that CEs derived from C. obtusa are dominantly composed of terpinen-4-ol (18.0%), α-terpinyl acetate (10.1%), bornyl acetate (9.7%), limonene (7.1%), and γ-terpinene (6.7%), while CEs derived from C. japonica are dominantly composed of terpinen-4-ol (48.0%) and α-pinene (15.9%), which exhibited robust antiviral activity against phi6 bacteriophage. Both CEs successfully inactivated the phi6 bacteriophage below the detection limit (10 PFU/mL) within a short exposure time of 30 s (log reduction value, LRV &gt; 4). Through exposure experiments utilizing CEs with content ratios prepared via 2-fold serial dilutions (ranging from 3.13% to 100%), we demonstrated that the antiviral effect could be sustained up to a concentration of 25% (C. obtusa LRV = 3.8, C. japonica LRV &gt; 4.3 at a 25% CE content ratio for each species). However, CEs with content ratios below 12.5% did not produce a significant reduction in bacteriophage concentration and consequently lost their antiviral effects. Conversely, both CEs did not exhibit antiviral activity against MS2 bacteriophage, a non-enveloped virus. Our findings reveal for the first time the potential of CEs derived from C. obtusa and C. japonica for use as antiviral agents specifically targeting enveloped viruses.

  23. Current and novel therapies for management of Acinetobacter baumannii -associated pneumonia 査読有り

    Aye Mya Sithu Shein, Parichart Hongsing, O’Rorke Kevin Smith, Phatthranit Phattharapornjaroen, Kazuhiko Miyanaga, Longzhu Cui, Hitoshi Ishikawa, Mohan Amarasiri, Peter N. Monk, Anthony Kicic, Tanittha Chatsuwan, Daniel Pletzer, Paul G. Higgins, Shuichi Abe, Dhammika Leshan Wannigama

    Critical Reviews in Microbiology 1-22 2024年7月

    出版者・発行元: Informa UK Limited

    DOI: 10.1080/1040841x.2024.2369948  

    ISSN:1040-841X

    eISSN:1549-7828

  24. Increased faecal shedding in SARS-CoV-2 variants BA.2.86 and JN.1 査読有り

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Puey Ounjai, Andrew C Singer, Naveen Kumar Devanga Ragupathi, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Tanittha Chatsuwan, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Richard Siow, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe, Hitoshi Ishikawa, Wanwara Thuptiang, Ali Hosseini Rad S.M., Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Phitsanuruk Kanthawee, Ratana Tacharoenmuang

    The Lancet Infectious Diseases 24 (6) e348-e350 2024年6月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/s1473-3099(24)00155-5  

    ISSN:1473-3099

  25. Multiple pathogen contamination of water, hands, and fomites in rural Nepal and the effect of WaSH interventions 査読有り

    Sital Uprety, Isaac Ngo, Marika Maggos, Bipin Dangol, Samendra P. Sherchan, Joanna L. Shisler, Mohan Amarasiri, Daisuke Sano, Thanh H. Nguyen

    International Journal of Hygiene and Environmental Health 257 114341-114341 2024年4月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.ijheh.2024.114341  

    ISSN:1438-4639

  26. Early treatment with fluvoxamine, bromhexine, cyproheptadine, and niclosamide to prevent clinical deterioration in patients with symptomatic COVID-19: a randomized clinical trial 査読有り

    Dhammika Leshan Wannigama, Cameron Hurst, Phatthranit Phattharapornjaroen, Parichart Hongsing, Natchalaikorn Sirichumroonwit, Kanokpoj Chanpiwat, Ali Hosseini Rad S.M., Robin James Storer, Puey Ounjai, Phitsanuruk Kanthawee, Natharin Ngamwongsatit, Rosalyn Kupwiwat, Chaisit Kupwiwat, James Michael Brimson, Naveen Kumar Devanga Ragupathi, Somrat Charuluxananan, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G. Higgins, Vishnu Nayak Badavath, Mohan Amarasiri, Valerie Verhasselt, Anthony Kicic, Tanittha Chatsuwan, Kashif Pirzada, Farid Jalali, Angela M. Reiersen, Shuichi Abe, Hitoshi Ishikawa, Chanikan Tanasatitchai, Supamat Amphol, Ladda Nantawong, Prangrawee Sangchan, Varissara Sinkajarern, Thutpharritchn Phoonakh, Phornnapat Utenpattanun, Aye Mya Sithu Shein, Timporn Vitoonpong, Nichapha Chongthavonsatit, Yahya Mankong, Piyapong Chaichana, Jenjira Yaithet, Dumrongsak Pongprajak, Sukjai Traimuangpak, Gasit Saksirisampant, Phimonsiri Lamloeskittinon, Adam Adam Hamdy, Sinthu Sinthu Kosasih, Sirirat Sirirat Luk-in

    eClinicalMedicine 70 102517-102517 2024年4月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.eclinm.2024.102517  

    ISSN:2589-5370

  27. Wastewater-based epidemiological surveillance of SARS-CoV-2 new variants BA.2.86 and offspring JN.1 in South and Southeast Asia 査読有り

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, W K C P Werawatte, S M Ali Hosseini Rad, Stefan Fernandez, Angkana T Huang, Porames Vatanaprasan, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Ratana Tacharoenmuang, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Bernadina Cynthia, John Jefferson V Besa, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Andrew C Singer, Tanittha Chatsuwan, Sam Trowsdale, Takashi Furukawa, Kazunari Sei, Daisuke Sano, Hitoshi Ishikawa, Kenji Shibuya, Aisha Khatib, Shuichi Abe, Parichart Hongsing

    Journal of Travel Medicine 31 (4) 2024年3月4日

    出版者・発行元: Oxford University Press (OUP)

    DOI: 10.1093/jtm/taae040  

    ISSN:1195-1982

    eISSN:1708-8305

    詳細を見る 詳細を閉じる

    Discover the shifting landscape of SARS-CoV-2 variants from October to December 2023, with JN.1 dominating South and Southeast Asia wastewater samples, increasing from &amp;lt;10% to &amp;gt;90%. Experience the dynamic evolution of viral strains in this period.

  28. Exploring indoor and outdoor dust as a potential tool for detection and monitoring of COVID-19 transmission 査読有り

    Suparinthon Anupong, Sudarat Chadsuthi, Parichart Hongsing, Cameron Hurst, Phatthranit Phattharapornjaroen, Ali Hosseini Rad S.M., Stefan Fernandez, Angkana T. Huang, Porames Vatanaprasan, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Natharin Ngamwongsatit, Vishnu Nayak Badavath, Wanwara Thuptimdang, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Kazuhiko Miyanaga, Longzhu Cui, Asuka Nanbo, Kenji Shibuya, Rosalyn Kupwiwat, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Paul G. Higgins, Anthony Kicic, Andrew C. Singer, Tanittha Chatsuwan, Sam Trowsdale, Shuichi Abe, Hitoshi Ishikawa, Mohan Amarasiri, Charin Modchang, Dhammika Leshan Wannigama

    iScience 27 (3) 109043-109043 2024年3月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.isci.2024.109043  

    ISSN:2589-0042

  29. Recommendations of Key Elements within an Integrated Monitoring Framework of Antimicrobial Resistance for Asian Countries 査読有り

    Ryo Honda, Manish Kumar, Mardalisa, Rongxuan Wang, Muhammad Adnan Sabar, Tushara Chaminda, Kwanrawee Sirikanchana, Prasert Makkeaw, Sulfikar, Feng Ju, Guangming Jiang, Bing Li, Chart Chiemchaisri, Ryota Gomi, Mohan Amarasiri, Henrietta Venter, Masateru Nishiyama, Toru Watanabe, Masaru Ihara, Ikuro Kasuga, Kozo Watanabe, Satoru Suzuki

    Environmental Science &amp; Technology Letters 11 (1) 5-8 2023年12月26日

    出版者・発行元: American Chemical Society (ACS)

    DOI: 10.1021/acs.estlett.3c00820  

    ISSN:2328-8930

    eISSN:2328-8930

  30. Novel intranasal phage-CaEDTA-ceftazidime/avibactam triple combination therapy demonstrates remarkable efficacy in treating Pseudomonas aeruginosa lung infection 査読有り

    Aye Mya Sithu Shein, Dhammika Leshan Wannigama, Cameron Hurst, Peter N. Monk, Mohan Amarasiri, Vishnu Nayak Badavath, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Wanwara Thuptimdang, Sirirat Luk-in, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Naris Kueakulpattana, Matchima Laowansiri, Tingting Liao, Rosalyn Kupwiwat, Rojrit Rojanathanes, Natharin Ngamwongsatit, Arsa Thammahong, Hitoshi Ishikawa, Daniel Pletzer, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Pattama Wapeesittipan, S.M. Ali Hosseini Rad, Talerngsak Kanjanabuch, Robin James Storer, Kazuhiko Miyanaga, Longzhu Cui, Hiroshi Hamamoto, Paul G. Higgins, Anthony Kicic, Tanittha Chatsuwan, Parichart Hongsing, Shuichi Abe

    Biomedicine &amp; Pharmacotherapy 168 115793-115793 2023年12月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.biopha.2023.115793  

    ISSN:0753-3322

  31. Modeling vaccination strategies with limited early COVID-19 vaccine access in low- and middle-income countries: A case study of Thailand 査読有り

    Suparinthon Anupong, Tanakorn Chantanasaro, Chaiwat Wilasang, Natcha C. Jitsuk, Chayanin Sararat, Kan Sornbundit, Busara Pattanasiri, Dhammika Leshan Wannigama, Mohan Amarasiri, Sudarat Chadsuthi, Charin Modchang

    Infectious Disease Modelling 8 (4) 1177-1189 2023年12月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.idm.2023.11.003  

    ISSN:2468-0427

  32. Tracing the new SARS-CoV-2 variant BA.2.86 in the community through wastewater surveillance in Bangkok, Thailand 査読有り

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Puey Ounjai, Ratana Tacharoenmuang, Naveen Kumar Devanga Ragupathi, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Andrew C Singer, Tanittha Chatsuwan, Sam Trowsdale, Aisha Khatib, Kenji Shibuya, Shuichi Abe, Hitoshi Ishikawa, Parichart Hongsing, Wanwara Thuptiang, Ali Hosseini Rad S M, Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Phitsanuruk Kanthawee

    The Lancet Infectious Diseases 23 (11) e464-e466 2023年11月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/s1473-3099(23)00620-5  

    ISSN:1473-3099

  33. Tracing the transmission of mpox through wastewater surveillance in Southeast Asia 査読有り

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, Wanwara Thuptimdang, S M Ali Hosseini Rad, Stefan Fernandez, Angkana T Huang, Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Andrew C Singer, Tanittha Chatsuwan, Sam Trowsdale, Richard Siow, Kenji Shibuya, Shuichi Abe, Hitoshi Ishikawa, Parichart Hongsing

    Journal of Travel Medicine 30 (5) 2023年7月18日

    出版者・発行元: Oxford University Press (OUP)

    DOI: 10.1093/jtm/taad096  

    ISSN:1195-1982

    eISSN:1708-8305

    詳細を見る 詳細を閉じる

    Abstract High population density and tourism in Southeast Asia increase the risk of mpox due to frequent interpersonal contacts. Our wastewater surveillance in six Southeast Asian countries revealed positive signals for Monkeypox virus (MPXV) DNA, indicating local transmission. This alerts clinicians and helps allocate resources like testing, vaccines and therapeutics in resource-limited countries.

  34. Ca-EDTA restores the activity of ceftazidime-avibactam or aztreonam against carbapenemase-producing Klebsiella pneumoniae infections 査読有り

    Dhammika Leshan Wannigama, Aye Mya Sithu Shein, Cameron Hurst, Peter N. Monk, Parichart Hongsing, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Pattama Wapeesittipan, Sirirat Luk-in, Sasipen Sae-Joo, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Naris Kueakulpattana, Matchima Laowansiri, Tingting Liao, Rosalyn Kupwiwat, Rojrit Rojanathanes, Natharin Ngamwongsatit, Somkanya Tungsanga, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Vishnu Nayak Badavath, S.M. Ali Hosseini Rad, Talerngsak Kanjanabuch, Nattiya Hirankarn, Robin James Storer, Longzhu Cui, Mohan Amarasiri, Hitoshi Ishikawa, Paul G. Higgins, Stephen M. Stick, Anthony Kicic, Tanittha Chatsuwan, Shuichi Abe

    iScience 26 (7) 107215-107215 2023年7月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.isci.2023.107215  

    ISSN:2589-0042

  35. COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities 査読有り

    Dhammika Leshan Wannigama, Mohan Amarasiri, Parichart Hongsing, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Phatthranit Phattharapornjaroen, Ali Hosseini Rad S. M., Stefan Fernandez, Angkana T. Huang, Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Nattiya Hirankarn, Paul G. Higgins, Anthony Kicic, Andrew C. Singer, Tanittha Chatsuwan, Sam Trowsdale, Shuichi Abe, Alexander D. McLellan, Hitoshi Ishikawa

    iScience 26 (7) 107019-107019 2023年7月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.isci.2023.107019  

    ISSN:2589-0042

  36. Microbial assessment of water, sanitation, and hygiene (WaSH) in temporary and permanent settlements two years after Nepal 2015 earthquake 査読有り

    Sital Uprety, Samendra P. Sherchan, Preeti Narayanan, Bipin Dangol, Marika Maggos, Alex Celmer, Joanna Shisler, Mohan Amarasiri, Daisuke Sano, Thanh H. Nguyen

    Science of The Total Environment 877 162867-162867 2023年6月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.scitotenv.2023.162867  

    ISSN:0048-9697

  37. Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia 査読有り

    Dhammika Leshan Wannigama, Mohan Amarasiri, Parichart Hongsing, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Phatthranit Phattharapornjaroen, Ali Hosseini Rad S.M., Stefan Fernandez, Angkana T. Huang, Naris Kueakulpattana, Chanikan Tanasatitchai, Porames Vatanaprasan, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Nattiya Hirankarn, Paul G. Higgins, Anthony Kicic, Tanittha Chatsuwan, Alexander D. McLellan, Shuichi Abe

    Science of The Total Environment 858 159816-159816 2023年2月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.scitotenv.2022.159816  

    ISSN:0048-9697

  38. Wastewater-based epidemiological surveillance of SARS-CoV-2 reveals silent variant transmission with future outbreaks in low-resource communities

    Mohan Amarasiri

    Respirology 2023年

  39. High prevalence of mgrB-mediated colistin resistance among carbapenem-resistant Klebsiella pneumoniae is associated with biofilm formation, and can be overcome by colistin-EDTA combination therapy 査読有り

    Mohan Amarasiri

    Scientific Reports 12 (1) 2022年12月

    DOI: 10.1038/S41598-022-17083-5  

    ISSN:2045-2322

    eISSN:2045-2322

  40. Concentration and reduction efficiency of vancomycin-resistant heterotrophic bacteria and vanA and vanB genes in wastewater treatment unit processes 査読有り

    Takashi Furukawa, Tohru Mekata, Mohan Amarasiri, Kazunari Sei

    Journal of Global Antimicrobial Resistance 30 340-347 2022年9月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.jgar.2022.07.008  

    ISSN:2213-7165

  41. Prevalence of antibiotic resistance genes in drinking and environmental water sources of the Kathmandu Valley, Nepal 査読有り

    Mohan Amarasiri, Tsubasa Takezawa, Bikash Malla, Takashi Furukawa, Jeevan B. Sherchand, Eiji Haramoto, Kazunari Sei

    Frontiers in Microbiology 13 2022年8月22日

    出版者・発行元: Frontiers Media SA

    DOI: 10.3389/fmicb.2022.894014  

    eISSN:1664-302X

    詳細を見る 詳細を閉じる

    Antibiotic-resistant bacteria-associated infections are responsible for more than 1.2 million annual deaths worldwide. In low- and middle-income countries (LMICs), the consumption of antibiotics for human and veterinary uses is not regulated effectively. Overused and misused antibiotics can end up in aquatic environments, which may act as a conduit for antibiotic resistance dissemination. However, data on the prevalence of antibiotic resistance determinants in aquatic environments are still limited for LMICs. In this study, we evaluated the prevalence and concentration of antibiotic resistance genes (ARGs) in different drinking and environmental water sources collected from the Kathmandu Valley, Nepal, using droplet digital polymerase chain reaction to understand the current situation of ARG contamination. River water and shallow dug well water sources were the most contaminated with ARGs. Almost all samples containedsul1(94%), andintI1andtet(A) were detected in 83 and 60% of the samples, respectively. Maximum ARG concentration varied between 4.2 log10copies/100 ml formecAand 9.3 log10copies/100 ml forsul1. Significant positive correlations were found between ARGs (r&amp;gt; 0.5,p&amp;lt; 0.01), except formecA, qnrS, andvanA. Assul1andintI1were detected in almost all samples, the presence of these genes in a given sample may need to be considered as background antibiotic resistance in LMICs. Therefore, monitoring of ARGs, such as β-lactam ARGs, quinolone resistance genes, and vancomycin resistance genes, may provide a better picture of the antibiotic resistance determinants in aquatic environments of LMICs.

  42. Inactivation of antibiotic resistant bacteria and their resistance genes in sewage by applying pulsed electric fields 査読有り

    Takashi Furukawa, Takahisa Ueno, Mina Matsumura, Mohan Amarasiri, Kazunari Sei

    Journal of Hazardous Materials 424 127382-127382 2022年2月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.jhazmat.2021.127382  

    ISSN:0304-3894

  43. Summary and Perspectives on Current Disinfection Technologies in Reducing Antibiotic Resistant Bacteria and Their Resistance Genes 査読有り

    TAKASHI FURUKAWA, MOHAN AMARASIRI, TAKAHISA UENO, KAZUNARI SEI

    Japanese Journal of Water Treatment Biology 58 (1) 9-24 2022年

    出版者・発行元: Japanese Society of Water Treatment Biology

    DOI: 10.2521/jswtb.58.9  

    ISSN:0910-6758

    eISSN:1881-0438

  44. Multidrug-resistant Neisseria gonorrhoeae infection in heterosexual men with reduced susceptibility to ceftriaxone, first report in Thailand 査読有り

    Naris Kueakulpattana, Dhammika Leshan Wannigama, Sirirat Luk-in, Parichart Hongsing, Cameron Hurst, Vishnu Nayak Badavath, Piroon Jenjaroenpun, Thidathip Wongsurawat, Nipat Teeratakulpisan, Stephen J. Kerr, Shuichi Abe, Phatthranit Phattharapornjaroen, Aye Mya Sithu Shein, Thammakorn Saethang, Naphat Chantaravisoot, Mohan Amarasiri, Paul G. Higgins, Tanittha Chatsuwan

    Scientific Reports 11 (1) 2021年11月4日

    出版者・発行元: Springer Science and Business Media LLC

    DOI: 10.1038/s41598-021-00675-y  

    ISSN:2045-2322

    eISSN:2045-2322

  45. Pathogens and disease vectors/hosts monitoring in aquatic environments: Potential of using eDNA/eRNA based approach 査読有り

    Mohan Amarasiri, Takashi Furukawa, Fumiyuki Nakajima, Kazunari Sei

    Science of The Total Environment 796 148810-148810 2021年11月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.scitotenv.2021.148810  

    ISSN:0048-9697

  46. Tracking COVID-19 with wastewater to understand asymptomatic transmission

    Mohan Amarasiri

    INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES 108 296-299 2021年7月

    DOI: 10.1016/J.IJID.2021.05.005  

    ISSN:1878-3511 1201-9712

  47. Human norovirus disease burden of consuming Crassostrea gigas oysters: A case-study from Japan 査読有り

    Yo Ueki, Mohan Amarasiri, Sayaka Kamio, Akie Sakagami, Hiroshi Ito, Sital Uprety, Arief Nurul Umam, Takayuki Miura, Thanh H. Nguyen, Daisuke Sano

    Food Control 121 107556-107556 2021年3月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.foodcont.2020.107556  

    ISSN:0956-7135

  48. Viral Interference as a Factor of False-Negative in the Infectious Adenovirus Detection Using Integrated Cell Culture-PCR with a BGM Cell Line 査読有り

    Daisuke Sano, Ryosuke Watanabe, Wakana Oishi, Mohan Amarasiri, Masaaki Kitajima, Satoshi Okabe

    Food and Environmental Virology 13 (1) 84-92 2021年1月4日

    出版者・発行元: Springer Science and Business Media LLC

    DOI: 10.1007/s12560-020-09453-x  

    ISSN:1867-0334

    eISSN:1867-0342

  49. Updated research agenda for water, sanitation and antimicrobial resistance 査読有り

    Sano, D., Wester, A.L., Schmitt, H., Amarasiri, M., Kirby, A., Medlicott, K., de Roda Husman, A.M.

    Journal of Water and Health 18 (6) 858-866 2020年12月1日

    出版者・発行元: {IWA} Publishing

    DOI: 10.2166/wh.2020.033  

    ISSN:1996-7829 1477-8920

    eISSN:1996-7829

  50. The Effect of GD1a Ganglioside-Expressing Bacterial Strains on Murine Norovirus Infectivity 査読有り

    Yifan Zhu, Hiroki Kawai, Satoshi Hashiba, Mohan Amarasiri, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano

    Molecules 25 (18) 4084-4084 2020年9月7日

    出版者・発行元: MDPI AG

    DOI: 10.3390/molecules25184084  

    eISSN:1420-3049

    詳細を見る 詳細を閉じる

    In this study, we investigated the impact of GD1a-expressing bacterial strains on the infectivity of murine norovirus (MNV). Eligible bacterial strains were screened from a sewage sample using flow cytometry, and their genetic sequences of 16S rRNA were determined. The enzyme-linked immunosorbent assay (ELISA) was employed to analyze the binding between bacteria and MNV particles, and the plaque assay was used to assess the effects of GD1a-positive and negative strains on MNV infectivity. The result from ELISA shows that MNV particles are able to bind to both GD1a-positive and negative bacterial strains, but the binding to the GD1a-positive strain is more significant. The infectivity assay result further shows that the MNV infectious titer declined with an increasing concentration of GD1a-positive bacteria. The addition of anti-GD1a antibody in the infectivity assay led to the recovery of the MNV infectious titer, further confirming that the binding between MNV particles and bacterial GD1a ganglioside compromises MNV infectivity. Our findings highlight the role indigenous bacteria may play in the lifecycle of waterborne enteric viruses as well as the potential of exploiting them for virus transmission intervention and water safety improvement.

  51. Identification of novel norovirus polymerase genotypes from pediatric fecal samples collected between the year 1997 and 2000 in Japan 査読有り

    Mohan Amarasiri, Etsuko Utagawa, Daisuke Sano, Kazuhiko Katayama

    Infection, Genetics and Evolution 82 104313-104313 2020年8月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.meegid.2020.104313  

    ISSN:1567-1348

  52. Assessment of microbial risks by characterization of Escherichia coli presence to analyze the public health risks from poor water quality in Nepal 査読有り

    Sital Uprety, Bipin Dangol, Pramina Nakarmi, Isha Dhakal, Samendra P. Sherchan, Joanna L. Shisler, Antarpreet Jutla, Mohan Amarasiri, Daisuke Sano, Thanh H. Nguyen

    International Journal of Hygiene and Environmental Health 226 113484-113484 2020年5月

    出版者・発行元: Elsevier BV

    DOI: 10.1016/j.ijheh.2020.113484  

    ISSN:1438-4639

  53. Virucidal Efficacy of Olanexidine Gluconate as a Hand Antiseptic Against Human Norovirus 査読有り

    Kaoru Imai, Akifumi Hagi, Yasuhide Inoue, Mohan Amarasiri, Daisuke Sano

    Food and Environmental Virology 12 (2) 180-190 2020年3月2日

    出版者・発行元: Springer Science and Business Media LLC

    DOI: 10.1007/s12560-020-09422-4  

    ISSN:1867-0334

    eISSN:1867-0342

    詳細を見る 詳細を閉じる

    Abstract Human noroviruses are the major cause of non-bacterial acute gastroenteritis worldwide. Since no therapeutic agent has been proven to prevent human norovirus infection yet, preventive healthcare interventions to block the infection routes play an important role in infection control. One of the possible infection routes of human noroviruses are through contaminated hands, but no hand antiseptics have been proven effective. Olanexidine gluconate is a new biguanide compound that has already been approved for sale as an antiseptic for the surgical field in Japan. A new hand antiseptic was developed using olanexidine gluconate in this study, and its virucidal efficacy against human noroviruses was evaluated using modified RT-qPCR that can account for genome derived from intact viruses using RNase A and photo-reactive intercalators. We tested the virucidal efficacy of five materials; two olanexidine gluconate antiseptics (hand rub formulation and surgical field formulation), two kinds of ethanol solutions at different pH (approx. 3 or 7), and a base component of olanexidine gluconate hand rub formulation against 11 human norovirus genotypes by culture-independent methods. The infectivity of murine norovirus (MNV), a surrogate for human norovirus, was significantly reduced after use of the antiseptics. The olanexidine gluconate hand rub demonstrated the strongest virucidal efficacy against human norovirus among the five tested materials. This study showed that olanexidine gluconate has the potential to become a strong tool for the prevention of human norovirus infection.

  54. Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered 査読有り

    Mohan Amarasiri, Daisuke Sano, Satoru Suzuki

    Critical Reviews in Environmental Science and Technology 50 (19) 1-44 2019年12月3日

    出版者・発行元: Informa {UK} Limited

    DOI: 10.1080/10643389.2019.1692611  

  55. Fecal Source Tracking in A Wastewater Treatment and Reclamation System Using Multiple Waterborne Gastroenteritis Viruses 査読有り

    Pathogens 8 (4) 170-170 2019年9月30日

    出版者・発行元:

    DOI: 10.3390/pathogens8040170  

  56. Sign-constrained linear regression for prediction of microbe concentration based on water quality datasets 国際誌 査読有り

    Journal of Water and Health 17 (3) 404-415 2019年6月

    DOI: 10.2166/wh.2019.001  

  57. Specific Interactions between Human Norovirus and Environmental Matrices: Effects on the Virus Ecology 査読有り

    Viruses 2019年3月5日

    DOI: 10.3390/v11030224  

  58. Specific interactions of rotavirus HAL1166 with Enterobacter cloacae SENG-6 and their contribution on rotavirus HAL1166 removal 査読有り

    Mohan Amarasiri, Hiroki Kawai, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano

    Water Science and Technology 2019年1月

    出版者・発行元: {IWA} Publishing

    DOI: 10.2166/wst.2019.050  

    ISSN:0273-1223

  59. Reverse transcription-quantitative PCR assays for genotype-specific detection of human noroviruses in clinical and environmental samples 査読有り

    Amarasiri M, Kitajima M, Miyamura A, Santos R, Monteiro S, Miura T, Kazama S, Okabe S, Sano D

    International Journal of Hygiene and Environmental Health 221 (3) 578-585 2018年4月

    出版者・発行元: Elsevier {BV}

    DOI: 10.1016/j.ijheh.2018.02.008  

  60. Bacteriophage removal efficiency as a validation and operational monitoring tool for virus reduction in wastewater reclamation: Review 査読有り

    Mohan Amarasiri, Masaaki Kitajima, Thanh H.Nguyen, Satoshi Okabe, Daisuke Sano

    Water Research 121 258-269 2017年9月

    出版者・発行元:

    DOI: 10.1016/j.watres.2017.05.035  

  61. Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane 査読有り

    Mohan Amarasiri, Satoshi Hashiba, Takayuki Miura, Toyoko Nakagomi, Osamu Nakagomi, Satoshi Ishii, Satoshi Okabe, Daisuke Sano

    Water Research 95 383-391 2016年5月

    出版者・発行元: Elsevier {BV}

    DOI: 10.1016/j.watres.2016.04.018  

  62. Risk management of viral infectious diseases in wastewater reclamation and reuse: Review 査読有り

    Daisuke Sano, Mohan Amarasiri, Akihiko Hata, Toru Watanabe, Hiroyuki Katayama

    Environment International 91 220-229 2016年5月

    出版者・発行元:

    DOI: 10.1016/j.envint.2016.03.001  

  63. Genome Sequence ofEnterobacter cloacaeStrain SENG-6, a Bacterium Producing Histo-Blood Group Antigen-Like Substances That Can Bind with Human Noroviruses 査読有り

    Satoshi Ishii, Mohan Amarasiri, Satoshi Hashiba, Peiyi Yang, Satoshi Okabe, Daisuke Sano

    Genome Announcements 4 (4) e00893-16 2016年

    出版者・発行元: American Society for Microbiology

    DOI: 10.1128/genomeA.00893-16  

  64. Ciprofloxacin resistant bacteria in the waterways surrounding Peradeniya, Sri Lanka

    Mohan Amarasiri

    Sri Lankan Journal of Infectious Diseases

    DOI: 10.4038/sljid.v13i5.8633  

︎全件表示 ︎最初の5件までを表示

講演・口頭発表等 108

  1. Environmental DNA analysis for the rodents monitoring in the urban drains and environmental samples

    Shiori Kambara, Takashi Furukawa, Mohan Amarasiri, Fumiyuki Nakajima, Noboru Yaguchi, Kazunari Sei

    IWA World Water Congress and Exhibition

  2. Surveillance of antibiotic resistant bacteria and their resistance genes in the wastewater treatment plants in Japan

    Miku Kanazashi, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    IWA World Water Congress and Exhibition

  3. Dynamics of class 1 integrons in wastewater treatment plants for the evaluation of the proliferation and occurrence of antibiotic resistant bacteria

    Kaho Misawa, Tomoya Yamamoto, Mohan Amarasiri, Takashi Furukawa, Shotaro Maehana, Daisuke Sano, Kazunari Sei

    Water and Environment Technology Conference 2024 (WET2024)

  4. Establishment of a method for the detection of rodents by environmental DNA analysis and its application to various urban environmental samples

    Shiori Kambara, Takashi Furukawa, Mohan Amarasiri, Fumiyuki Nakajima, Noboru Yaguchi, Kazunari Sei

    Water and Environment Technology Conference 2024 (WET2024)

  5. Comparative study on the characteristics of antibiotic resistant bacteria and their resistance genes in the wastewater treatment plants in Kanagawa, Japan

    Miku Kanazashi, Mohan Amarasiri, Shotaro Maehana, Takashi Furukawa, Kazunari Sei

    Water and Environment Technology Conference 2024 (WET2024)

  6. Surveillance on dynamic patterns of antibiotic resistant bacteria and multi-drug resistance in Sagami River, Japan

    E. A. C. Priyadarshani, Mizuki Ogino, Mohan Amarasiri, Tatsuru Kamei, Takashi Furukawa, Kazunari Sei

    Environmental Dimension of Antimicrobial Resistance Conference 2024 (EDAR7)

  7. Analysis of antimicrobial resistance genes and plasmid transmissibility in Aeromonas sp. carrying blaIMP-1 isolated from hospital wastewater

    Mako Koyama, Nagi Niida, Ryotaro Eda, Masato Suzuki, Hiroki Izawa, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei, Makoto Kubo, Shotaro Maehana

    Environmental Dimension of Antimicrobial Resistance Conference 2024 (EDAR7)

  8. Dynamics of class 1 integrons in wastewater treatment plants and hospital wastewater

    Kaho Misawa, Tomoya Yamamoto, Mohan Amarasiri, Shotaro Maehana, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    Environmental Dimension of Antimicrobial Resistance Conference 2024 (EDAR7)

  9. intI1遺伝子を指標とした都市下水および病院排水中における薬剤耐性遺伝子の存在実態推定

    三澤香穂, 山本智也, Mohan Amarasiri, 古川隼士, 前花祥太郎, 佐野大輔, 清和成

    第58回日本水環境学会年会

  10. 環境DNA分析による都市の各種排水および環境試料中の鼠族モニタリング

    神原栞, 古川隼士, Mohan Amarasiri, 中島典之, 矢口昇, 清和成

    第58回日本水環境学会年会

  11. 神奈川県下の2ヶ所の下水処理場における下水処理前後の薬剤耐性菌と耐性遺伝子の比較調査

    金刺未来, Mohan Amarasiri, 古川隼士, 前花祥太郎, 清和成

    第58回日本水環境学会年会

  12. パルス電界応用技術によるバクテリオファージの不活化効果の検証

    諸石涼羽, 木下樺子, 古川隼士, 上野崇寿, Mohan Amarasiri, 清和成

    第58回日本水環境学会年会

  13. パルス電界印加技術によるエアロゾル感染性ウイルスの不活化効果の検証

    諸石涼羽, 木下樺子, 古川隼士, 上野崇寿, Mohan Amarasiri, 清和成

    第60回環境工学研究フォーラム

  14. パルス電界を用いた水耕栽培養液中の植物病原体不活化効果の検証

    古川隼士, 野崎正洋, 上野崇寿, Mohan Amarasiri, 清和成

    第60回環境工学研究フォーラム

  15. intI1遺伝子を指標とした下水処理場での薬剤耐性遺伝子削減効果の評価

    三澤香穂, 山本智也, Mohan Amarasiri, 古川隼士, 前花祥太郎, 佐野大輔, 清和成

    第59回日本水処理生物学会大会

  16. 環境DNA解析による鼠族検出法の確立と都市の各種排水への適用

    神原栞, 古川隼士, Mohan Amarasiri, 矢口昇, 中島典之, 清和成

    第59回日本水処理生物学会大会

  17. パルス電界応用技術による空気感染性ウイルスの不活化効果の検証

    諸石涼羽, 木下樺子, 上野崇寿, 古川隼士, Mohan Amarasiri, 清和成

    第59回日本水処理生物学会大会

  18. 環境DNAおよび環境RNA定量解析によるマンソン住血吸虫中間宿主貝検出の時空間的感度評価

    竹澤翼, Mohan Amarasiri, 古川隼士, 清和成

    第59回日本水処理生物学会大会

  19. Studying the temporal and spatial variations of antibiotic resistant bacteria in Sagami River

    E.A.C. Priyadarshani, Mizuki Ogino, Tatsuru Kamei, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    The 59th Japanese Society for Water Treatment Biology Conference

  20. 神奈川県下の2ヶ所の下水処理場における流入水および放流水中の薬剤耐性菌と耐性遺伝子の特徴

    金刺未来, Mohan Amarasiri, 古川隼士, 前花祥太郎, 清和成

    第59回日本水処理生物学会大会

  21. Establishment of a simple rodent monitoring method based on environmental DNA analysis and its application for the rodents monitoring in the drains

    Shiori Kambara, Takashi Furukawa, Mohan Amarasiri, Fumiyuki Nakajima, Kazunari Sei

    The 9th IWA-ASPIRE Conference & Exhibition

  22. Surveillance of antibiotic resistant bacteria and their resistance genes in the wastewater treatment plants in Kanagawa

    Miku Kanazashi, Mohan Amarasiri, Shotaro Maehana, Takashi Furukawa, Kazunari Sei

    The 9th IWA-ASPIRE Conference & Exhibition

  23. Recovery and inactivation of pathogenic bacteria in aerosols by applying pulsed electric field

    Suzuha Moroishi, Takashi Furukawa, Takahisa Ueno, Mohan Amarasiri, Kazunari Sei

    The 9th IWA-ASPIRE Conference & Exhibition

  24. 水環境における薬剤耐性菌の発生源と挙動解明の試み~水環境中の薬剤耐性菌はどこからやってくるのか?

    清和成, Mohan Amarasiri, 古川隼士

    第26回日本水環境学会シンポジウム

  25. Verification and inactivation effect of aerosol-infectious virus by pulsed electric field application technology

    Suzuha Moroishi, Kako Kinoshita, Takashi Furukawa, Takahisa Ueno, Mohan Amarasiri, Kazunari Sei

    15th International Joint Workshop on Advanced Engineering Technology for Environment and Energy

  26. Establishment of a simple rodent monitoring method based on environmental DNA analysis and its application for the rodent monitoring in drains

    Shiori Kambara, Takashi Furukawa, Mohan Amarasiri, Fumiyuki Nakajima, Kazunari Sei

    15th International Joint Workshop on Advanced Engineering Technology for Environment and Energy

  27. Antibiotic resistance profile and the prevalence of six major β-lactamase gene subtypes in Sagami River

    E.A.C. Priyadarshani, Mizuki Ogino, Mohan Amarasiri, Tatsuru Kamei, Takashi Furukawa, Kazunari Sei

    15th International Joint Workshop on Advanced Engineering Technology for Environment and Energy

  28. Investigation of antimicrobial resistant bacteria and their resistance profiles in Sagami River basin

    E.A.C. Priyadarshani, Mizuki Ogino, Mohan Amarasiri, Tatsuru Kamei, Takashi Furukawa, Kazunari Sei

    Water and Environment Technology Conference 2023 (WET2023)

  29. Establishment of a rodent detection system by Environmental DNA analysis and its application to the urban drains

    Shiori Kambara, Takashi Furukawa, Mohan Amarasiri, Fumiyuki Nakajima, Kazunari Sei

    Water and Environment Technology Conference 2023 (WET2023)

  30. Surveillance of antibiotic resistant bacteria and their resistance genes in the wastewater treatment plants in Kanagawa, Japan

    Miku Kanazashi, Mohan Amarasiri, Shotaro Maehana, Takashi Furukawa, Kazunari Sei

    Water and Environment Technology Conference 2023 (WET2023)

  31. Prevalence of antibiotic resistance genes in aquatic samples and their reduction after a WaSH intervention

    Mohan Amarasiri, Sital Uprety, Chihiro Konuma, Takashi Furukawa, Kazunari Sei, Helen Nguyen, Daisuke Sano

    The 21st IWA Symposium on Health-Related Water Microbiology

  32. Temporal dynamics of class 1 integrons in hospital wastewater

    Mohan Amarasiri, Tomoya Yamamoto, Shotaro Maehana, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    The 21st IWA Symposium on Health-Related Water Microbiology

  33. 病院排水より分離された広域β-ラクタムおよびチゲサイクリン耐性菌の存在実態と耐性遺伝子解析

    石村菜穂子, 江田諒太郎, 伊澤紘輝, Mohan Amarasiri, 古川隼士, 清和成, 久保誠, 前花祥太郎

    第57回日本水環境学会年会

  34. 病院排水中における薬剤耐性遺伝子の水平伝播に影響を及ぼすインテグロン動態の解明

    山本智也, Mohan Amarasiri, 前花祥太郎, 古川隼士, 佐野大輔, 清和成

    第57回日本水環境学会年会

  35. パルス高電界を用いた水耕栽培養液の消毒効果の評価

    野崎正洋, 古川隼士, 上野崇寿, Mohan Amarasiri, 清和成

    第57回日本水環境学会年会

  36. 環境DNA/RNA解析による鼠族検出系の確立と病院排水管からの鼠族検出への適用

    神原栞, 古川隼士, Mohan Amarasiri, 中島典之, 清和成

    第57回日本水環境学会年会

  37. 神奈川県下の2ヶ所の下水処理場における流入水および放流水中の薬剤耐性菌と耐性遺伝子の存在実態調査

    金刺未来, Mohan Amarasiri, 古川隼士, 前花祥太郎, 清和成

    第57回日本水環境学会年会

  38. パルス高電界によるエアロゾル中の細菌の回収・不活化

    諸石涼羽, 古川隼士, 上野崇寿, Mohan Amarasiri, 清和成

    第57回日本水環境学会年会

  39. 過酢酸、過ギ酸による下水二次処理水中の細胞外薬剤耐性遺伝子の不活化評価

    小林大起, Mohan Amarasiri, 古川隼士, 佐野大輔, 清和成

    第57回日本水環境学会年会

  40. 相模川における薬剤耐性菌と薬剤耐性遺伝子の存在実態と耐性プロファイルの調査

    荻野瑞葵, 金刺未来, 亀井樹, Mohan Amarasiri, 古川隼士, 清和成

    第57回日本水環境学会年会

  41. 結合塩素による薬剤耐性遺伝子の接合伝達効率への影響評価

    森山桃子, Mohan Amarasiri, 鈴木聡, 佐野大輔

    第57回日本水環境学会年会

  42. Detection of antibiotic resistance genes (ARGs) in drinking and environmental water sources of the Kathmandu Valley, Nepal using droplet digital PCR

    Tsubasa Takezawa, Mohan Amarasiri, Bikash Malla, Takashi Furukawa, Jeevan Sherchand, Eiji Haramoto, Kazunari Sei

    14th International Joint Workshop on Advanced Engineering Technology for Environment and Energy 2022年10月15日

  43. Inactivation of extracellular antibiotic resistance genes using peracetic acid (PAA) and performic acid (PFA)

    Hiroki Kobayashi, Mohan Amarasiri, Ryuji Ampo, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    14th International Joint Workshop on Advanced Engineering Technology for Environment and Energy 2022年10月15日

  44. Monitoring and characterization of antibiotic resistant bacteria in Sagami River for the identification of their origins

    Mizuki Ogino, Miku Kanazashi, Shiori Kambara, Tatsuru Kamei, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    14th International Joint Workshop on Advanced Engineering Technology for Environment and Energy 2022年10月15日

  45. Identification of possible origins of ARB in river environments

    Mizuki Ogino, Miku Kanazashi, Shiori Kambara, Tatsuru Kamei, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    6th International Symposium on the Environmental Dimension of Antibiotic Resistance (EDAR6)

  46. Prevalence of antibiotic resistance genes in drinking and environmental water sources of the Kathmandu Valley, Nepal

    Tsubasa Takezawa, Mohan Amarasiri, Bikash Malla, Takashi Furukawa, Jeevan Sherchand, Eiji Haramoto, Kazunari Sei

    IWA World Water Congress & Exhibition

  47. Disinfection of extracellular antibiotic resistance genes using peracetic acid (PAA) and performic acid (PFA)

    Hiroki Kobayashi, Mohan Amarasiri, Ryuji Ampo, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    IWA World Water Congress & Exhibition

  48. Disinfection of extracellular antimicrobial resistant genes (ARGs) using free chlorine (Cl), peracetic acid (PAA), and performed acid (PFA)

    Hiroki Kobayashi, Mohan Amarasiri, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    Water and Environment Technology Conference 2022 (WET2022)

  49. Abundance of antimicrobial resistant bacteria and their resistance profiles in Sagami River basin

    Mizuki Ogino, Miku Kanazashi, Shiori Kambara, Tatsuru Kamei, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    Water and Environment Technology Conference 2022 (WET2022)

  50. Investigation of antibiotic resistance genes in river water and household water in the Kathmandu Valley, Nepal

    Tsubasa Takezawa, Mohan Amarasiri, Bikash Malla, Takashi Furukawa, Eiji Haramoto, Kazunari Sei

    Water and Environment Technology Conference 2022 (WET2022)

  51. Capture and inactivation of fecal indicator bacteria in aerosols by applying pulsed electric field

    Suzuha Moroishi, Takashi Furukawa, Mohan Amarasiri, Takahisa Ueno, Kazunari Sei

    Water and Environment Technology Conference 2022 (WET2022)

  52. ネパール・カトマンズ盆地の河川水・生活用水の薬剤耐性遺伝子汚染の実態調査

    竹澤翼, Mohan Amarasiri, 古川隼士, Bikash Malla, 原本英司, 清和成

    第56回日本水環境学会年会

  53. 水環境条件下における薬剤耐性遺伝子の接合伝達効率に影響を与える環境因子に関する研究

    森山桃子, Amarasiri Mohan, 鈴木聡, 佐野大輔

    第56回日本水環境学会年会

  54. 簡易消毒による環境中の細胞外薬剤耐性遺伝子への物理的な消毒効果及び形質転換効率への影響の比較評価

    小林大起, 安保龍二, Mohan Amarasiri, 古川隼士, 佐野大輔, 清和成

    第56回日本水環境学会年会

  55. PCR screening reveals the existence of quinolone resistance gens in anthropogenically affected waterways of Badulla and Kandy Districts

    M.H.M.I.M. Gunawardane, C.D. Gamage, S. Rajapakse, G.M.T.M.Herath, A.K.U.I. Karunadasa, E.W.M.A.Ekanayake, Takashi Furukawa, Mohan Amarasiri, Kazunari Sei

    Postgraduate Institute of Science Research Congress

  56. Abundance of antimicrobial resistant bacteria and their antimicrobial resistant profiles in hospital sewage

    Jo Yonemoto, Mizuki Ogino, Nagi Niida, Ryotaro Eda, Shotaro Maehana, Mohan Amarasiri, Takashi Furukawa, Hidero Kitasato, Kazunari Sei

    Water and Environment Technology conference 2021 (WET2021)

  57. Abundance of antimicrobial resistant bacteria and their resistance profiles in Sagami River basin

    Mizuki Ogino, Yukiko Kobayashi, Tatsuru Kamei, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    Water and Environment Technology conference 2021 (WET2021)

  58. Inactivation efficiency assessment of antimicrobial resistance genes (ARGs) existing as extracellular DNA using simple disinfection methods

    Hiroki Kobayashi, Mohan Amarasiri, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    Water and Environment Technology conference 2021 (WET2021)

  59. 病院排水中の薬剤耐性菌の存在実態と耐性プロファイルの調査

    米本譲, 荻野瑞葵, 小林由紀子, 新井田凪, 江田諒太郎, 前花祥太郎, Amarasiri Mohan, 古川隼士, 北里英郎, 清和成

    第55回日本水環境学会年会

  60. 環境DNAとして存在する薬剤耐性遺伝子への簡易消毒による不活化効果の評価

    小林大起, Amarasiri Mohan, 古川隼士, 佐野大輔, 清和成

    第55回日本水環境学会年会

  61. 相模川における薬剤耐性菌の存在実態と耐性プロファイルの調査

    荻野瑞葵, 米本譲, 小林由紀子, 亀井樹, Amarasiri Mohan, 古川隼士, 清和成

    第55回日本水環境学会年会

  62. 水環境条件下における薬剤耐性遺伝子の接合伝達効率に関する研究

    森山桃子, Mohan Amarasiri, 鈴木聡, 佐野大輔

    第55回日本水環境学会年会

  63. パルス電界印加技術を用いた薬剤耐性菌および耐性遺伝子の不活化効果の検証

    松村美那, 古川隼士, 上野崇寿, Amarasiri Mohan, 清和成

    第55回日本水環境学会年会

  64. 環境DNA/環境RNA解析による衛生動物検出の時間的感度評価

    須江渚, 大知鼓太郎, 飯塚洸平, 前田倖, Amarasiri Mohan, 古川隼士, 中島典之, 清和成

    第55回日本水環境学会年会

  65. 薬剤耐性遺伝子による生活環境の汚染に対するWASH介入効果の評価

    小沼千紘, Mohan Amarasiri, Sital Uprety, Thanh Nguyen, 鈴木聡, 佐野大輔

    第55回日本水環境学会年会

  66. パルス電界印加技術を応用した薬剤耐性菌および耐性遺伝子の削減効果

    松村美那, 古川隼士, 上野崇寿, Amarasiri Mohan, 清和成

    第57回環境工学研究フォーラム

  67. 環境RNA解析による衛生動物の高感度検出手法の開発および時間的感度の評価

    須江渚, 古川隼士, 中島典之, Amarasiri Mohan, 清和成

    第57回環境工学研究フォーラム

  68. Evaluation of microbial health risks caused by the usage of river water for a decentralized drinking water system

    Arief Umam, Syun-suke Kadoya, Mohan Amarasiri, Daisuke Sano

    The 23rd Symposium of Japanese Society for Water Environment

  69. 自然条件下における水中腸管系ウイルス自然死滅モデルの構築

    加藤郁生, モハン アマラシリ, 佐野大輔

    第54回日本水環境学会年会

  70. 水環境サンプルからの形質転換可能な細胞外薬剤耐性遺伝子の回収方法の確立

    小沼千紘, モハン アマラシリ, 鈴木聡, 佐野大輔

    第54回日本水環境学会年会

  71. Histo-blood group antigen-like substances bearing bacteria are prevalent in wastewater 招待有り

    Mohan Amarasiri

    The22nd U.S.-Japan Cooperative Medical Sciences Program (USJCMSP), Viral Diseases Panel Meeting

  72. Antibiotic-induced Changes in Enterobacter cloacae SENG-6 Extracellular Polymeric Substances

    Mohan Amarasiri, Hui Cheng, Yu-You Li, Daisuke Sano

    The 8th IWA-Microbial Ecology and Water Engineering Specialist Conference

  73. Microbial Assessment of Water, Sanitation and Hygiene (WASH) Two Years After Nepal 2015 Earthquake

    Sital Uprety, Mohan Amarasiri, Bipin Dangol, Daisuke Sano, Helen Nguyen

    The 8th IWA-Microbial Ecology and Water Engineering Specialist Conference

  74. Human health risk of Legionella pneumophila infection caused by the groundwater usage in a decentralized drinking water system

    Arief Umam, Mohan Amarasiri, Daisuke Sano

    The 7th International Symposium on Water Environment Systems 2019年11月15日

  75. Water, Sanitation and Hygiene (WaSH) assessments two years after Nepal 2015 Earthquake

    Sital Uprety, Mohan Amarasiri, Bipin Dangol, Daisuke Sano, Helen Nguyen

    World Bosai Forum 2019

  76. Quantification of human health risks caused by antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Future research directions 招待有り

    Mohan Amarasiri

    The 6th Environmental Technology and Management Conference

  77. Impact of Water, Sanitation, and Hygiene (WASH) interventions on the bacterial pathogen load in households in rural Nepal

    Sital Uprety, Mohan Amarasiri, Bipin Dangol, Daisuke Sano, Helen Nguyen

    IWA Symposium on Health-Related Water Microbiology

  78. Extracellular antibiotic resistance gene decay by free chlorine treatment 国際会議

    Mohan Amarasiri, Chihiro Konuma, Daisuke Sano

    IWA Health Related Water Microbiology

  79. Quantitative microbial risk assessment for users of a decentralized drinking water system

    Arief Umam, Mohan Amarasiri, Daisuke Sano

    The 6th Symposium for Environmental Water Quality Engineering 2019年6月22日

  80. 細胞外薬剤耐性遺伝子の発現に与える遊離塩素の影響

    小沼千紘, 門屋俊祐, モハン アマラシリ, 佐野大輔

    第6回環境水質工学シンポジウム 2019年6月22日

  81. 水中病原ウイルスの自然死滅モデルパラメータ推定式の構築

    加藤郁生, モハン アマラシリ, 佐野大輔

    第53回日本水環境学会年会

  82. 細胞外薬剤耐性遺伝子の遊離塩素による消毒効果の評価

    小沼千紘, モハン アマラシリ, 門屋俊祐, 佐野大輔

    平成30年度土木学会東北支部技術研究発表会 2019年3月2日

  83. Identification of bacterial genes for producing norovirus-binding glycoconjugate 国際会議

    Amarasiri, M, Yang, P.Y, Kitajima, M, Kasahara, Y, Okabe, S, Sano, D

    6th Food and Environmental Virology Conference 2018年10月7日

  84. Contribution of specific interactions between human enteric viruses and wastewater solids on virus removal 国際会議

    Amarasiri, M, Kawai, H, Kitajima, M, Okabe, S, Sano, D

    IWA world water congress and exhibition 2018年9月16日

  85. Screening of bacterial genes responsible for producing norovirus-binding glycoconjugate 国際会議

    Amarasiri, M, Yang, P.Y, Kitajima, M, Kasahara, Y, Okabe, S, Sano, D

    Water and environment technology (WET) conference 2018年7月14日

  86. Genotype-dependent behaviors of human noroviruses in aquatic environments 国際会議 招待有り

    Amarasiri, M

    Symposium on risk reduction of infectious diseases from different perspectives 2018年3月20日

  87. 腸内細菌の組織血液型決定抗原様物質分泌関連遺伝子に関する研究

    小島友祐, モハン・アマラシリ, 佐野大輔

    日本大学郡山キャンパス 2018年3月3日

  88. 病原ウイルスの自然死滅モデルに関する研究

    加藤郁生, 稲葉愛美, モハン・アマラシリ, 佐野大輔

    日本大学郡山キャンパス 2018年3月3日

  89. Human enteric virus removal from wastewater: Design and operational monitoring of multiple-barrier system and virus removal efficiency improvement using specific interaction 国際会議

    Amarasiri, M

    20th Symposium of Japanese Society for Water Environment 2017年9月26日

  90. Histo-blood group antigen (HBGA) positive bacteria in the mixed liquor reduces the human rotavirus concentration in membrane filtration effluent 国際会議

    Amarasiri, M, Kawai, H, Kitajima, M, Okabe, S, Sano, D

    Water and environment technology (WET) conference 2017年7月22日

  91. Removal of rotavirus by microfiltration in the presence of HBGA-positive bacteria 国際会議

    Amarasiri, M, Kawai, H, Kitajima, M, Okabe, S, Sano, D

    4th Symposium for Environmental Water Quality Engineering 2017年6月17日

  92. 環境水中からのノロウイルス吸着性大腸菌の単離と吸着因子同定に関する研究

    羽柴聡, AMARASIRI Mohan, 原本英司, 北島正章, 岡部聡, 佐野大輔

    第51回日本水環境学会年会

  93. Effects of wastewater solids-virus interactions on virus removal and survival in wastewater 国際会議 招待有り

    Amarasiri, M

    Japan-YWP International forum on water treatment technology 2017年2月20日

  94. Human glycan-positive bacteria as an adsorbent for norovirus 国際会議

    Amarasiri, M, Hashiba, S, Okabe, S, Miyoshi, T, Tanaka, T, Sano, D

    64th Annual Meeting of The Japanese Society for Virology 2016年10月23日

  95. Diversity of histo-blood group antigen-positive enteric bacteria as a specific adsorbent for human noroviruses

    Daisuke Sano, Mohan Amarasiri, Satoshi Hashiba, Takatomo Ohta, Takayuki Miura, Toyoko Nakagomi, Osamu Nakagomi, Satoshi Okabe

    The 6th International Calicivirus Conference

  96. A systematic review and meta-analysis on the virus removal efficiencies of wastewater treatment plant unit processes 国際会議

    Amarasiri, M, Sano, D, Hata, A, Watanabe, T, Katayama, H

    5th Food and Environmental Virology Conference 2016年9月13日

  97. Genotype-dependent norovirus removal by microfiltration: Influence of bacterial histo-blood group antigens 国際会議

    Amarasiri, M, Hashiba, S, Okabe, S, Sano, D

    3rd Symposium for Environmental Water Quality Engineering 2016年5月21日

  98. Effect of localization of HBGA-like substances on membrane separation on norovirus 国際会議

    Amarasiri, M, Hashiba, S, Okabe, S, Sano, D

    3rd International Life-Science Symposium 2015年11月26日

  99. Diverse enteric bacteria can excrete histo-blood group antigens as adsorbents for human gastroenteritis viruses 国際会議

    Sano, D, Ohta, T, Amarasiri, M, Nakagomi, T, Miura, T, Nakagomi, O, Okabe, S

    63rd Annual Meeting of the Japanese Society for Virology 2015年11月22日

  100. Diversity of histo-blood group antigen-positive enteric bacteria in water: possible environmental vehicle for human norovirus 国際会議

    Sano, D, Ohta, T, Amarasiri, M, Miura, T, Nakagomi, T, Nakagomi, O, Okabe, S

    18th International symposium on health-related water microbiology 2015年9月13日

  101. Microfiltration of human noroviruses with blood-type active enteric bacteria

    Amarasiri, M, Yoshimura, T, Okabe, S, Sano, D

    22nd Public Health Engineering Symposium 2014年11月21日

  102. Genotype-dependent membrane separation of human noroviruses with bacterial histo-blood group antigen (HBGA)-like substances 国際会議

    Amarasiri, M, Yoshimura, T, Nakagomi, T, Nakagomi, O, Okabe, S, Sano, D

    4th Food and Environmental Virology Conference 2014年9月2日

  103. Development of a screening assay of norovirus-binding enteric bacteria bearing histo-blood group antigen-like substances 国際会議

    Sano, D, Amarasiri, M, Yoshimura, T, Nakagomi, T, Nakagomi, O, Okabe, S

    International Union of Microbiological Societies 2014年7月27日

  104. Membrane separation of human noroviruses in combination with enteric bacteria bearing histo-blood group antigen (HBGA)-like substances 国際会議

    Amarasiri, M, Yoshimura, T, Okabe, S, Sano, D

    IWA specialist conference on advances in particle science and separation

  105. Norovirus removal with microfiltration in the presence of HBGA-like substances-bearing bacteria: A new approach to improve removal efficiency 国際会議

    Amarasiri, M, Yoshimura, T, Nakagomi, T, Nakagomi, O, Okabe, S, Sano, D

    The 1st Symposium for Environmental Water Quality Engineering 2014年5月9日

  106. Human enteric bacteria-receptors of human norovirus and implications in microfiltration

    Amarasiri, M, Sano, D, Yoshimura, T, Ishizaki, S, Nakagomi, T, Nakagomi, O, Okabe, S

    21st Public Health Engineering Symposium 2013年12月4日

  107. Microfiltration of human noroviruses: Effects of the presence of human enteric bacteria on virus rejection 国際会議

    Amarasiri, M, Sano, D, Yoshimura, T, Ishizaki, S, Nakagomi, T, Nakagomi, O, Okabe, S

    Water and environment technology (WET) conference 2013年6月15日

  108. Risk to Groundwater from Improper Toilet Waste Disposals

    Mohan Amarasiri, Gemunu Herath, Tharanga Jayathilaka, Chatura Weerasinghe

    International Symposium on Water Quality and Human Health: Challenges Ahead

︎全件表示 ︎最初の5件までを表示

共同研究・競争的資金等の研究課題 8

  1. ワンヘルス・アプローチに基づいた薬剤耐性菌・耐性遺伝子の発生源対策技術戦略の構築

    清 和成, 上野 崇寿, 米加田 徹, 前花 祥太郎, Amarasiri Mohan, 臼井 優, 亀井 樹, 鈴木 祥広, 古川 隼士

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research

    研究種目:Grant-in-Aid for Scientific Research (A)

    研究機関:Kitasato University

    2023年4月1日 ~ 2026年3月31日

  2. 薬剤耐性菌の排出負荷低減に資する高電圧パルス電界を応用した下水消毒システムの構築

    古川 隼士, 上野 崇寿, 前花 祥太郎, Amarasiri Mohan, 清 和成

    2023年4月1日 ~ 2026年3月31日

  3. スリランカにおける下水水質情報に基づいた下水管路劣化予測システムの開発

    佐野 大輔, 水谷 大二郎, Amarasiri Mohan

    2021年10月7日 ~ 2024年3月31日

    詳細を見る 詳細を閉じる

    本研究は、スリランカにおいて、微生物の起源を同定する手法であるMicrobial Source Tracking(MST)の手法を応用し、下水管の破損部分から混入する土壌由来の微生物の下水中に占める割合を空間的・時間的に把握することで、下水管破損箇所を推定し、かつ下水管劣化速度の予測に適用するための新規手法を開発することを目指すものである。研究初年度である令和3年度においては、まずスリランカ側カウンターパート(チャンディカ・ガマゲ(ペラデニヤ大学医学部・講師)、及びチャミンダ・サマラスーリヤ(モラトゥワ大学地球資源工学部・講師))とのオンライン打ち合わせを通じ、スリランカ国内における調査地点の確定を行った。希望する調査地点で下水採取を行うために、所轄官庁向けの要望書を作成・提出したところである。さらに、本研究で想定している手法が適用可能であることを確認するために、日本国内で未処理下水を採取し、総DNAを抽出して、細菌の分類に用いられる16s rRNA遺伝子配列の取得を行った。その結果、下水に特徴的な細菌種の16s rRNA遺伝子配列が確実に得られることを確認することに成功し、スリランカで得られたサンプルに適用する準備が整えられた。

  4. 環境RNA解析による時間的感度を考慮した都市衛生動物の高感度検出・定量法の開発

    清 和成, 中島 典之, 古川 隼士, Amarasiri Mohan

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research

    研究種目:Grant-in-Aid for Challenging Research (Pioneering)

    研究機関:Kitasato University

    2020年7月30日 ~ 2023年3月31日

    詳細を見る 詳細を閉じる

    本研究では、世界中で度々問題となっている各種の感染症を媒介する衛生動物を、環境RNA分析によって効率的にモニタリングする手法の開発を目的として、【課題1】都市生態系水試料中の環境DNA/RNA濃縮・回収手法の確立、【課題2】標的衛生動物由来環境RNAの特異的高感度検出・定量法の確立、【課題3】標的衛生動物由来環境RNAの特異的高感度検出・定量法の時間的感度評価の3課題に取り組んでいる。 2021年度は標的衛生動物由来環境RNAの特異的高感度検出・定量法の時間的感度評価を実施した。具体的には、河川を想定した流水系実験水槽として4L水槽を3つ連結し(それぞれ上流、中流、下流水槽)、モデル衛生動物としてマンソン住血吸虫の中間宿主貝を中流水槽で7日間飼育後、中間宿主貝を全除去、経時的に各水槽から水試料を採取して、環境DNAおよび環境RNAを回収し、中間宿主貝由来のDNAおよびRNAをPCRおよび逆転写PCRで検出した。検出の時間的感度を中間宿主貝の飼育前後でのPCRおよび逆転写PCRの結果から、空間的感度を各水槽での検出結果の比較から評価した。時間的感度に関しては、環境DNAでは中間宿主貝の除去後も3週間にわたって中間宿主貝由来のDNAが検出されたのに対し、環境RNAでは中間宿主貝除去後2日目には検出されなくなった。また、空間的感度に関しては、環境DNAでは中流水槽に加えて下流水槽からも中間宿主貝由来のDNAが検出されたのに対し、環境RNAでは中流水槽のみから中間宿主貝由来のRNAが検出され、実験期間を通して下流水槽からは検出されなかった。これより、環境RNA解析が時間的にも空間的にも、より高感度な標的衛生動物を検出できることを明らかにした。

  5. ソーストラッキングによる薬剤耐性菌および耐性遺伝子の発生源と環境中挙動の解明

    清 和成, Amarasiri Mohan, 亀井 樹, 古川 隼士

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research

    研究種目:Grant-in-Aid for Scientific Research (B)

    研究機関:Kitasato University

    2020年4月1日 ~ 2023年3月31日

    詳細を見る 詳細を閉じる

    近年世界中で問題となっている薬剤耐性菌(ARB)および薬剤耐性遺伝子(ARGs)は、下水処理場がその主要な水環境への排出源の1つとされているが、下水処理場流入水にも、無視できない割合でARBが存在していることが明らかとなっている。また、実際に下水処理場から流出するARBやARGsの環境中での挙動については、ほとんど明らかになっていない。 本研究では、都市下水、畜産排水、農業排水や病院排水を受け入れている下水処理場とその放流先河川を対象に、下水処理場に流入してくるARBやARGsの発生源および下水処理場から流出するARBやARGsの水環境中挙動を明らかにすることで、都市水環境を対象としたARBやARGsの発生源対策ならびにARBやARGsによる公衆衛生上の問題解決に資する基盤データを得ることを目的としている。 2021年度は山梨・神奈川両県を流れる相模川流域の6地点から、夏季、秋季、冬季の3回サンプリングを実施した。また、神奈川県下の病院排水のサンプリングを毎月実施し、各サンプルから従属栄養細菌を約100株ずつランダムに釣菌し、17種類の薬剤に対する感受性試験を実施した。 相模川では、2020年度秋季と同様に、従属栄養細菌数は下流に向かって増加していくのに対して、薬剤耐性菌数は下流に向かって減少する傾向が常時確認され、その95%以上が2種類以上の抗菌薬に耐性を示す多剤耐性菌であった。また、病院排水では、すべての抗菌薬に対して感受性を示した細菌株はなく、すべての細菌株が2種類以上の抗菌薬に対して耐性を示したこと、11種類の薬剤に対する耐性を示す株も3株検出され、病院排水がARBの重要な排出源の1 つであることを明らかにした。ただし、病院排水と河川水から検出される薬剤耐性の遺伝子型は必ずしも一致せず、河川水の薬剤耐性菌汚染の主因は病院排水とは異なる可能性を見出した。

  6. パルス電界法を応用した新規消毒技術の確立とその消毒メカニズムの解明に関する研究

    古川 隼士, 上野 崇寿, 清 和成, 亀井 樹, Amarasiri Mohan

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research

    研究種目:Grant-in-Aid for Scientific Research (C)

    研究機関:Kitasato University

    2019年4月1日 ~ 2022年3月31日

    詳細を見る 詳細を閉じる

    我々は、インパルス電界(PEF)印加消毒を応用して薬剤耐性菌(ARB)および耐性遺伝子(ARGs)の不活化も可能とする消毒技術の確立を目指し、本研究では、PEF印加消毒によるARBおよびARGsの不活化効果を明らかにすることを目的とした。PEF印加消毒は、バンコマイシン耐性腸球菌(VRE)だけでなくvanA遺伝子も不活化できることが明らかにした。VREは検出下限値以下まで削減された(5log以上)一方で、vanA遺伝子も4log以上の削減率を示したが、処理水中には105 copies/mLオーダーで残存したことから、PEF印加消毒においてARGsはARBよりも不活化されにくいことが示唆された。

  7. 薬剤耐性遺伝子による水環境汚染の実態解明と環境中遺伝子不活化手法の開発

    Mohan Amarasiri

    提供機関:Japan Society for the Promotion of Science

    制度名:Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    研究種目:Grant-in-Aid for Early-Career Scientists

    研究機関:Tohoku University

    2020年4月 ~ 2022年3月

    詳細を見る 詳細を閉じる

    本研究では塩素、過酢酸、過ギ酸を用いた簡易消毒による薬剤耐性遺伝子(ARGs)不活化効果の評価を目的として、モデルARGにアンピシリン(Amp)耐性遺伝子をコードしたプラスミドpUC19を用い、その簡易消毒処理による物理的破壊の可能性をPCRで検証するとともに、大腸菌への形質転換効率を求めることでARGsの不活化効果を比較・評価した。 遊離塩素は、1ppmの濃度でも時間依存性の高い消毒効果を示した。PAAおよびPFAの場合、不活化効率は初期濃度とともに増加し、時間依存性はほとんど無視できた。 pUC19プラスミドへの物理的損傷が主要な不活化メカニズムであった。

  8. Effects of antibiotics in wastewater on the removal of human enteric viruses by specific interaction between virus and wastewater solids 競争的資金

    モハン アマラシリ

    提供機関:Japanese Society for the Promotion of Science

    制度名:Kakenhi

    研究種目:Grant-in-Aid for Early-Career Scientists

    研究機関:Tohoku University

    2018年4月 ~ 2020年3月

    詳細を見る 詳細を閉じる

    アミカシンとクロラムフェニコールのEnterobacter cloacaeに対するMICをE―テストを使用して評価した。その後、細菌を抑制レベル以下の薬剤濃度で培養した。 SMP、LB-EPS、TB-EPSの生産を抽出して抽出された画分の炭水化物およびタンパク質濃度を測定した。励起発光マトリックス蛍光分光法によりEPS構造の変化を観察しました。その後、異なる薬剤濃度で増殖させたE.cloacaeの存在下でロタウイルスHAL1166の精密ろ過実験を行いました。 「水環境における薬剤耐性菌と薬剤耐性遺伝子によるヒトの健康リスクの理解:現在の知識と回答すべき質問」論文が公開された。

︎全件表示 ︎最初の5件までを表示

担当経験のある科目(授業) 12

  1. 環境保健学概論 北里大学

  2. サスティナビリティ学入門 北里大学

  3. 生物・生態環境工学 北里大学

  4. 環境衛生学 北里大学

  5. 微生物学 北里大学

  6. 環境衛生学・工学実習 北里大学

  7. 生物・生態環境工学 北里大学

  8. 微生物学演習 北里大学

  9. 機器分析化学実習 北里大学

  10. 健康科学演習 北里大学

  11. 水環境デザイン演習Ⅱ 東北大学

  12. 建築・社会環境工学演習 (BG) 東北大学

︎全件表示 ︎最初の5件までを表示