Details of the Researcher

PHOTO

Amarasiri Kalahe Panditha Koralage Mohan
Section
Graduate School of Engineering
Job title
Associate Professor
Degree

  • Ph.D. (Hokkaido University)

  • M. Eng. (Hokkaido University)

  • BSc Eng (University of Peradeniya)

e-Rad No.
50815537

Research History 3

  • 2024/04 - Present
    Tohoku University　Graduate School of Engineering　Associate Professor

  • 2020/04 - 2024/03
    Kitasato University　School of Allied Health Sciences　Lecturer

  • 2017/10 - 2020/03
    Tohoku University　Graduate School of Engineering　Assistant Professor

Education 3

  • Hokkaido University　Division of Environmental Engneering　Ph.D.

    2014/10 - 2017/09

  • Hokkaido University　Division of Environmental Engineering　M. Eng.

    2012/10 - 2014/09

  • University of Peradeniya　Civil Engineering　B Sc Eng

    2007/03 - 2011/09

Committee Memberships 2

  • Japanese Society on Water Environment　JWET Committee

    2023/07 - Present

  • International Society for Food and Environmental Virology Conference 2024　Organizing Committee

    2023/03 - 2024/05

Professional Memberships 3

  • International Water Association

  • Institute of Engineers, Sri Lanka.

  • JAPAN SOCIETY ON WATER ENVIRONMENT

Research Interests 5

  • enteric bacteria

  • antimicrobial resistance

  • Histo-blood group antigens

  • enteric virus

  • Wastewater treatment

Research Areas 3

  • Life sciences / Molecular biology /

  • Environmental science/Agricultural science / Environmental load reduction/restoration technology / Antimicrobial resistance

  • Social infrastructure (civil Engineering, architecture, disaster prevention) / Civil engineering (environmental systems) / Wastewater treatment

Awards 5

  1. Best Teacher 2021 Award

    2022/03　Kitasato University, Department of Health Science

  2. Best Paper Award

    2021/04　Critical Reviews in Environmental Science and Technology

  3. Best Presentation Award

    2019/09　International Water Association Health Related Water Microbiology Conference

  4. JSWE-Organo doctoral research award

    2017/09/26　Japanese Society on Water Environment

  5. Excellent Presentation Award

    2013/05　Japan Society on Water Environment

Papers 64

  1. Linking heavy metal(loid) stress to biofilm formation and conjugation: A critical review of experimental evidence

    Katayoun Dadeh Amirfard, Mohan Amarasiri, Daisuke Sano

    Critical Reviews in Environmental Science and Technology　2026/05/06

    DOI： 10.1080/10643389.2026.2666100 　

  2. Emergence of Tigecycline‐Resistant Pseudomonas aeruginosa Harbouring tmexC6D6‐toprJ1b From Hospital Sewage in Japan

    Shotaro Maehana, Masato Suzuki, Naoko Ishimura, Hiroki Izawa, Ryotaro Eda, Masaki Nakamura, Mohan Amarasiri, Takashi Furukawa, Fumiaki Kojima, Kazunari Sei, Makoto Kubo

    Environmental Microbiology Reports　2026/04

    DOI： 10.1111/1758-2229.70275 　

  3. Inactivation of bio-aerosolized viruses by applying pulsed electric field

    Takashi Furukawa, Takahisa Ueno, Suzuha Moroishi, Yuki Hoshiko, Shotaro Maehana, Mohan Amarasiri, Kazunari Sei

    Environmental Pollution　2026/04

    DOI： 10.1016/j.envpol.2026.127841 　

  4. Energy allocation trade-offs among conjugative transfer, biofilm formation, and heavy metal resistance: a dynamic energy budget theory perspective

    Katayoun Dadeh Amirfard, Mohan Amarasiri, Daisuke Sano

    Water Research　2026/03

    DOI： 10.1016/j.watres.2025.125216 　

  5. From wastewater to epidemiological insights: A systematic review of modeling strategies for infectious disease surveillance

    Luyao Wang, Mohan Amarasiri, Wakana Oishi, Daisuke Sano

    Water Research　2026/01

    DOI： 10.1016/j.watres.2025.124977 　

  6. Intranasal phage therapy overcomes antibody neutralization challenges in pulmonary Pseudomonas aeruginosa infections. International-journal

    Dhammika Leshan Wannigama, Jingping Shao, Hongya Sun, Yangzhong Wang, Cameron Hurst, Peter N Monk, Mohan Amarasiri, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Thin Sandi Htun, Sirirat Luk-In, Yoshitaka Shimotai, Natharin Ngamwongsatit, Hitoshi Ishikawa, Naveen Kumar Devanga Ragupathi, Daniel Pletzer, Talerngsak Kanjanabuch, Aisha Khatib, Kazuhiko Miyanaga, Longzhu Cui, Kenji Shibuya, Paul G Higgins, Anthony Kicic, Parichart Hongsing, Jinxin Zhao, Shuichi Abe, Hiroshi Hamamoto

    Archives of microbiology　207　(12)　310-310　2025/10/18

    DOI： 10.1007/s00203-025-04526-6 　

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    Phage therapy is a promising approach against multidrug-resistant infections, yet systemic administration can lead to incomplete cures. We investigated the distribution, immune responses, and efficacy of the therapeutic phage KPP10 delivered via intranasal or intraperitoneal (IP) routes in murine Pseudomonas aeruginosa lung infection models. Intranasal pre-treatment achieved markedly higher localization of KPP10 in the lungs and bronchoalveolar compartment compared to IP delivery. Intranasal administration elicited minimal systemic antibody responses, whereas IP injection triggered significant IgG, IgM, and IgA production. Antibody responses did not differ significantly between doses. In acute and chronic infection models, intranasal KPP10 significantly improved survival (p < 0.01) and reduced lung bacterial loads relative to IP injection. Importantly, IP treatment was associated with bacterial rebound after day 14 in chronic infection, whereas intranasal dosing sustained bacterial clearance. These findings demonstrate that intranasal delivery enhances pulmonary localization, minimizes antibody-mediated neutralization, and provides superior therapeutic efficacy, highlighting its potential as a more effective route for phage therapy against P. aeruginosa lung infections.

  7. Streptococcus pyogenes Surveillance Through Surface Swab Samples to Track the Emergence of Streptococcal Toxic Shock Syndrome in Rural Japan

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Yu Suzuki, Kazunori Moriya, Kazuhiko Miyanaga, Longzhu Cui, Angkana T Huang, Yoshikazu Okuma, Daisuke Akaneya, Junko Igarashi, Mayu Suto, Daisuke Ishizawa, Wakana Imamiya, Ayaka Igarashi, Yoshitaka Shimotai, Andrew C Singer, Naveen Kumar Devanga Ragupathi, Takashi Furukawa, Kazunari Sei, Yangzhong Wang, Talerngsak Kanjanabuch, Paul G Higgins, Nobuhito Nemoto, Aisha Khatib, Anthony Kicic, Sam Trowsdale, Parichart Hongsing, Daisuke Sano, Kenji Shibuya, Shuichi Abe, Hiroshi Hamamoto

    The Journal of Infectious Diseases　2025/08/14

    DOI： 10.1093/infdis/jiaf234 　

  8. Usefulness of pulsed electric field application as an inactivation technology for plant pathogenic bacteria in hydroponic nutrient solutions

    Takashi Furukawa, Takahisa Ueno, Masahiro Nozaki, Ayumi Yoshida, Mohan Amarasiri, Kazunari Sei

    Environmental Technology &amp; Innovation　39　104237-104237　2025/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.eti.2025.104237 　

    ISSN： 2352-1864

  9. Global participatory wastewater surveillance to understand Mpox clade diversity in war and conflict-affected countries Peer-reviewed

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Yangzhong Wang, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Nada M Melhem, Andrew C Singer, Naveen Kumar Devanga Ragupathi, Juliana Calabria Araujo, Kazunari Sei, Lazarus G Ndatuwong, Syed Faisal Mahmood, Yoshitaka Shimotai, Talerngsak Kanjanabuch, Paul G Higgins, Tetsuji Aoyagi, Anthony Kicic, Asuka Nanbo, Hirotake Mori, Richard Siow, Özgür Kurt, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Daisuke Sano, Kenji Shibuya, Shuichi Abe, Hiroshi Hamamoto

    Journal of Travel Medicine　2025/06/02

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/jtm/taaf052 　

    ISSN： 1195-1982

    eISSN： 1708-8305

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    Teaser Community-led wastewater surveillance reveals widespread circulation of the mpox virus and clade diversity in conflict-affected countries. This low-cost approach fills critical surveillance gaps, uncovering regional clade variations, including the more severe Clade Ib, and highlighting the potential of decentralized genomic monitoring where traditional systems fail.

  10. Surveillance of avian influenza through bird guano in remote regions of the global south to uncover transmission dynamics Peer-reviewed

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, John Jefferson V. Besa, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T. Huang, Puey Ounjai, W. K. C. P. Werawatte, Ali Hosseini Rad S. M, Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Phitsanuruk Kanthawee, Wanwara Thuptimdang, Ratana Tacharoenmuang, Bernadina Cynthia, S. P. H. Spencer Vitharana, Natharin Ngamwongsatit, Hitoshi Ishikawa, Takashi Furukawa, Yangzhong Wang, Andrew C. Singer, Naveen Kumar Devanga Ragupathi, Tanittha Chatsuwan, Kazunari Sei, Asuka Nanbo, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Hiroshi Hamamoto, Paul G. Higgins, Daisuke Sano, Anthony Kicic, José O. Valdebenito, Jonas Bonnedahl, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe

    Nature Communications　16　(1)　2025/05/27

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41467-025-59322-z 　

    eISSN： 2041-1723

  11. Identifying High-Risk Bacteria with Active Nasal Swab Surveillance in Intensive Care Units to Prevent Ventilator-Associated Pneumonia

    Yu Kuramasu, Yu Suzuki, Daisuke Akaneya, Yoshikazu Okuma, Yuta Tsujimoto, Daisuke Ishizawa, Kazunori Moriya, Parichart Hongsing, Mohan Amarasiri, Cameron Hurst, Paul G. Higgins, Kenji Shibuya, Anthony Kicic, Yoshitaka Shimotai, Hiroshi Hamamoto, Dhammika Leshan Wannigama, Shuichi Abe

    International Journal of Translational Medicine　2025/04/25

    DOI： 10.3390/ijtm5020017 　

  12. tesG expression as a potential clinical biomarker for chronic Pseudomonas aeruginosa pulmonary biofilm infections. International-journal

    Dhammika Leshan Wannigama, Cameron Hurst, Peter N Monk, Gunter Hartel, William Graham Fox Ditcham, Parichart Hongsing, Phatthranit Phattharapornjaroen, Puey Ounjai, Pattama Torvorapanit, Kamonwan Jutivorakool, Sirirat Luk-In, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Kazuhiko Miyanaga, Longzhu Cui, Naveen Kumar Devanga Ragupathi, S M Ali Hosseini Rad, Aisha Khatib, Robin James Storer, Hitoshi Ishikawa, Mohan Amarasiri, Somrat Charuluxananan, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Jane C Davies, Stephen M Stick, Anthony Kicic, Tanittha Chatsuwan, Kenji Shibuya, Shuichi Abe

    BMC medicine　23　(1)　191-191　2025/03/31

    DOI： 10.1186/s12916-025-04009-x 　

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    BACKGROUND: Pseudomonas aeruginosa infections in the lungs affect millions of children and adults worldwide. To our knowledge, no clinically validated prognostic biomarkers for chronic pulmonary P. aeruginosa infections exist. Therefore, this study aims to identify potential prognostic markers for chronic P. aeruginosa biofilm lung infections. METHODS: Here, we screened the expression of 11 P. aeruginosa regulatory genes (tesG, algD, lasR, lasA, lasB, pelB, phzF, rhlA, rsmY, rsmZ, and sagS) to identify associations between clinical status and chronic biofilm infection. RESULTS: RNA was extracted from 210 sputum samples from patients (n = 70) with chronic P. aeruginosa lung infections (mean age; 29.3-56.2 years; 33 female). Strong biofilm formation was correlated with prolonged hospital stays (212.2 days vs. 44.4 days) and increased mortality (46.2% (18)). Strong biofilm formation is associated with increased tesG expression (P = 0.001), influencing extended intensive care unit (P = 0.002) or hospitalisation stays (P = 0.001), pneumonia risk (P = 0.006), and mortality (P = 0.001). Notably, tesG expression is linked to the modulation of systemic and sputum inflammatory responses and predicts biofilm biomass. CONCLUSIONS: This study provides the first clinical dataset of tesG expression levels as a predictive biomarker for chronic P. aeruginosa pulmonary infections.

  13. Aptamer-based biosensors for wastewater surveillance of influenza virus, SARS-CoV-2, and norovirus: A comprehensive review

    Yilei Wang, Mohan Amarasiri, Wakana Oishi, Masayasu Kuwahara, Yuka Kataoka, Hiroki Kurita, Fumio Narita, Rong Chen, Qian Li, Daisuke Sano

    Water Research　123484-123484　2025/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.watres.2025.123484 　

    ISSN： 0043-1354

  14. Combination of curcumin or chitosan with photodynamic therapy as an effective alternative therapy for overcoming wound infection associated with multidrug-resistant Acinetobacter baumannii

    Mohan Amarasiri

    MICROBE　2025

    DOI： 10.1016/J.MICROB.2025.100375 　

  15. Tapeworm infection incidence in rural Japan points to a common environmental source of infection

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Yu Suzuki, Daisuke Akaneya, Mika Moriya, Taichi Adachi, Yoshikazu Okuma, Daisuke Ishizawa, Hitoshi Ishikawa, Kazuhiko Miyanaga, Longzhu Cui, Kazunori Moriya, Hirotake Mori, Naveen Kumar Devanga Ragupathi, Yoshitaka Shimotai, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Talerngsak Kanjanabuch, Paul G. Higgins, Tetsuji Aoyagi, Anthony Kicic, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe, Hiroshi Hamamoto

    Drug Discoveries &amp; Therapeutics　2025

    Publisher: International Research and Cooperation Association for Bio & Socio-Sciences Advancement (IRCA-BSSA)

    DOI： 10.5582/ddt.2025.01070 　

    ISSN： 1881-7831

    eISSN： 1881-784X

  16. Phage cocktail amikacin combination as a potential therapy for bacteremia associated with carbapenemase producing colistin resistant Klebsiella pneumoniae. International-journal

    Aye Mya Sithu Shein, Dhammika Leshan Wannigama, Cameron Hurst, Peter N Monk, Mohan Amarasiri, Thidathip Wongsurawat, Piroon Jenjaroenpun, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Vishnu Nayak Badavath, Sirirat Luk-In, Sumanee Nilgate, Ubolrat Rirerm, Sukrit Srisakul, Naris Kueakulpattana, Matchima Laowansiri, S M Ali Hosseini Rad, Supaporn Wacharapluesadee, Apaporn Rodpan, Natharin Ngamwongsatit, Arsa Thammahong, Hitoshi Ishikawa, Robin James Storer, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Annika Y Classen, Talerngsak Kanjanabuch, Daniel Pletzer, Kazuhiko Miyanaga, Longzhu Cui, Hiroshi Hamamoto, Paul G Higgins, Anthony Kicic, Tanittha Chatsuwan, Parichart Hongsing, Shuichi Abe

    Scientific reports　14　(1)　28992-28992　2024/11/22

    DOI： 10.1038/s41598-024-79924-9 　

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    The increasing occurrence of hospital-associated infections, particularly bacteremia, caused by extensively drug-resistant (XDR) carbapenemase-producing colistin-resistant Klebsiella pneumoniae highlights a critical requirement to discover new therapeutic alternatives. Bacteriophages having host-specific bacteriolytic effects are promising alternatives for combating these pathogens. Among 12 phages isolated from public wastewater in Thailand, two phages-vB_kpnM_05 (myovirus) and vB_kpnP_08 (podovirus) showed broad-host range, producing bacteriolytic activities against 81.3% (n = 26) and 78.1% (n = 25) of 32 XDR carbapenemase-producing colistin-resistant K. pneumoniae, with capsular types-K15, K17, K50, K51, K52/wzi-50 and K2/wzi-2. Both phages showed short replication times, large burst sizes with rapid adsorptions. They exhibited significant stability under various environmental conditions. Genomic analysis revealed that both phages are genetically distinct phages from Myoviridae and Podoviridae family, with the lack of toxin, virulence, lysogeny and antibiotic resistance genes. These characteristics highlighted their promising potential for utilizing in phage therapy for combating XDR K. pneumoniae. Although phage cocktail combining vB_kpnM_05 and vB_kpnP_08 provided significant bacteriolysis for longer duration (8 h) than its monophage (6 h), bacterial regrowth was observed which suggested an evitable development of phage resistance under phages' selection pressures. Future study will be undertaken to elucidate the precise mechanisms by which these XDR K. pneumoniae developed phage resistance and their associated fitness cost. Remarkably, combining phage cocktail with amikacin at their sub-inhibitory concentrations produced potent synergy by completely suppressing bacterial regrowth in vitro. Our study demonstrated the significant therapeutic and prophylactic effectiveness of a phage cocktail-amikacin combination as a promising alternative strategy for overcoming bacteremia associated with XDR K. pneumoniae having carbapenemase and colistin resistance in vivo.

  17. Impact of COVID-19 Vaccination in Thailand: Averted Deaths and Severe Infections Across Age Groups

    Chaiwat Wilasang, Pikkanet Suttirat, Dhammika Leshan Wannigama, Mohan Amarasiri, Sudarat Chadsuthi, Charin Modchang

    Tropical Medicine and Infectious Disease　2024/11/22

    DOI： 10.3390/tropicalmed9120286 　

  18. Identification of protein biomarkers in wastewater linked to the incidence of COVID-19 Peer-reviewed

    Luhur Akbar Devianto, Mohan Amarasiri, Luyao Wang, Takehito Iizuka, Daisuke Sano

    Science of The Total Environment　951　175649-175649　2024/11

    DOI： 10.1016/j.scitotenv.2024.175649 　

    ISSN： 0048-9697

  19. Phage therapy could be key to conquering persistent bacterial lung infections in children. International-journal

    Aye Mya Sithu Shein, Parichart Hongsing, Aisha Khatib, Phatthranit Phattharapornjaroen, Kazuhiko Miyanaga, Longzhu Cui, Kenji Shibuya, Mohan Amarasiri, Peter N Monk, Anthony Kicic, Tanittha Chatsuwan, Paul G Higgins, Shuichi Abe, Dhammika Leshan Wannigama

    npj antimicrobials and resistance　2　(1)　31-31　2024/10/10

    DOI： 10.1038/s44259-024-00045-4 　

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    Persistent bacterial lung infections in children lead to significant morbidity and mortality due to antibiotic resistance. In this paper, we describe how phage therapy has shown remarkable efficacy in preclinical and clinical studies, demonstrating significant therapeutic benefits through various administration routes. Ongoing trials are evaluating its safety and effectiveness against different pathogens. Advancing phage therapy through systematic studies and international collaboration could provide a viable alternative to traditional antibiotics for persistent infections.

  20. Degradation of extracellular antibiotic resistance genes using peracetic acid (PAA) and performic acid (PFA) Peer-reviewed

    Mohan Amarasiri, Hiroki Kobayashi, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    Environmental Technology &amp; Innovation　36　103862-103862　2024/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.eti.2024.103862 　

    ISSN： 2352-1864

  21. Community-based mpox and sexually transmitted disease surveillance using discarded condoms in the global south Peer-reviewed

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, John Jefferson V Besa, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Puey Ounjai, W.K.C.P. Werawatte, Ali Hosseini Rad S.M., Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Phitsanuruk Kanthawee, Marily Al-Dois, Rapong Bani, Wanwara Thuptiang, Sifat Khan, Ratana Tacharoenmuang, Bernadina Cynthia, S.P.H Spencer Vitharana, Natharin Ngamwongsatit, Hitoshi Ishikawa, Andrew C Singer, Naveen Kumar Devanga Ragupathi, Takashi Furukawa, Kazunari Sei, Asuka Nanbo, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Tanittha Chatsuwan, Paul G Higgins, Daisuke Sano, Anthony Kicic, Jürgen Kurt Rockstroh, Richard Siow, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe

    The Lancet Infectious Diseases　24　(10)　e610-e613　2024/10

    Publisher: Elsevier BV

    DOI： 10.1016/s1473-3099(24)00514-0 　

    ISSN： 1473-3099

  22. Cell Extracts Derived from Cypress and Cedar Show Antiviral Activity against Enveloped Viruses Peer-reviewed

    Takashi Furukawa, Ayumu Inagaki, Takeshi Hatta, Suzuha Moroishi, Katsuki Kawanishi, Yuki Itoh, Shotaro Maehana, Mohan Amarasiri, Kazunari Sei

    Microorganisms　12　(9)　1813-1813　2024/09/02

    Publisher: MDPI AG

    DOI： 10.3390/microorganisms12091813 　

    eISSN： 2076-2607

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    The antiviral efficacy of cell-extracts (CEs) derived from cypress (Chamaecyparis obtusa (Siebold &amp; Zucc.) Endl., C. obtusa) and cedar (Cryptomeria japonica (Thunb. ex. L.) D.Don, C. japonica) was assessed using phi6 and MS2 bacteriophages, which are widely accepted surrogate models for enveloped and non-enveloped viruses, in order to verify their potential use as antiviral agents. Our results indicate that CEs derived from C. obtusa are dominantly composed of terpinen-4-ol (18.0%), α-terpinyl acetate (10.1%), bornyl acetate (9.7%), limonene (7.1%), and γ-terpinene (6.7%), while CEs derived from C. japonica are dominantly composed of terpinen-4-ol (48.0%) and α-pinene (15.9%), which exhibited robust antiviral activity against phi6 bacteriophage. Both CEs successfully inactivated the phi6 bacteriophage below the detection limit (10 PFU/mL) within a short exposure time of 30 s (log reduction value, LRV &gt; 4). Through exposure experiments utilizing CEs with content ratios prepared via 2-fold serial dilutions (ranging from 3.13% to 100%), we demonstrated that the antiviral effect could be sustained up to a concentration of 25% (C. obtusa LRV = 3.8, C. japonica LRV &gt; 4.3 at a 25% CE content ratio for each species). However, CEs with content ratios below 12.5% did not produce a significant reduction in bacteriophage concentration and consequently lost their antiviral effects. Conversely, both CEs did not exhibit antiviral activity against MS2 bacteriophage, a non-enveloped virus. Our findings reveal for the first time the potential of CEs derived from C. obtusa and C. japonica for use as antiviral agents specifically targeting enveloped viruses.

  23. Current and novel therapies for management of Acinetobacter baumannii -associated pneumonia Peer-reviewed

    Aye Mya Sithu Shein, Parichart Hongsing, O’Rorke Kevin Smith, Phatthranit Phattharapornjaroen, Kazuhiko Miyanaga, Longzhu Cui, Hitoshi Ishikawa, Mohan Amarasiri, Peter N. Monk, Anthony Kicic, Tanittha Chatsuwan, Daniel Pletzer, Paul G. Higgins, Shuichi Abe, Dhammika Leshan Wannigama

    Critical Reviews in Microbiology　1-22　2024/07

    Publisher: Informa UK Limited

    DOI： 10.1080/1040841x.2024.2369948 　

    ISSN： 1040-841X

    eISSN： 1549-7828

  24. Increased faecal shedding in SARS-CoV-2 variants BA.2.86 and JN.1 Peer-reviewed

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Puey Ounjai, Andrew C Singer, Naveen Kumar Devanga Ragupathi, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Tanittha Chatsuwan, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Richard Siow, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe, Hitoshi Ishikawa, Wanwara Thuptiang, Ali Hosseini Rad S.M., Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Phitsanuruk Kanthawee, Ratana Tacharoenmuang

    The Lancet Infectious Diseases　24　(6)　e348-e350　2024/06

    Publisher: Elsevier BV

    DOI： 10.1016/s1473-3099(24)00155-5 　

    ISSN： 1473-3099

  25. Multiple pathogen contamination of water, hands, and fomites in rural Nepal and the effect of WaSH interventions Peer-reviewed

    Sital Uprety, Isaac Ngo, Marika Maggos, Bipin Dangol, Samendra P. Sherchan, Joanna L. Shisler, Mohan Amarasiri, Daisuke Sano, Thanh H. Nguyen

    International Journal of Hygiene and Environmental Health　257　114341-114341　2024/04

    Publisher: Elsevier BV

    DOI： 10.1016/j.ijheh.2024.114341 　

    ISSN： 1438-4639

  26. Early treatment with fluvoxamine, bromhexine, cyproheptadine, and niclosamide to prevent clinical deterioration in patients with symptomatic COVID-19: a randomized clinical trial Peer-reviewed

    Dhammika Leshan Wannigama, Cameron Hurst, Phatthranit Phattharapornjaroen, Parichart Hongsing, Natchalaikorn Sirichumroonwit, Kanokpoj Chanpiwat, Ali Hosseini Rad S.M., Robin James Storer, Puey Ounjai, Phitsanuruk Kanthawee, Natharin Ngamwongsatit, Rosalyn Kupwiwat, Chaisit Kupwiwat, James Michael Brimson, Naveen Kumar Devanga Ragupathi, Somrat Charuluxananan, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G. Higgins, Vishnu Nayak Badavath, Mohan Amarasiri, Valerie Verhasselt, Anthony Kicic, Tanittha Chatsuwan, Kashif Pirzada, Farid Jalali, Angela M. Reiersen, Shuichi Abe, Hitoshi Ishikawa, Chanikan Tanasatitchai, Supamat Amphol, Ladda Nantawong, Prangrawee Sangchan, Varissara Sinkajarern, Thutpharritchn Phoonakh, Phornnapat Utenpattanun, Aye Mya Sithu Shein, Timporn Vitoonpong, Nichapha Chongthavonsatit, Yahya Mankong, Piyapong Chaichana, Jenjira Yaithet, Dumrongsak Pongprajak, Sukjai Traimuangpak, Gasit Saksirisampant, Phimonsiri Lamloeskittinon, Adam Adam Hamdy, Sinthu Sinthu Kosasih, Sirirat Sirirat Luk-in

    eClinicalMedicine　70　102517-102517　2024/04

    Publisher: Elsevier BV

    DOI： 10.1016/j.eclinm.2024.102517 　

    ISSN： 2589-5370

  27. Wastewater-based epidemiological surveillance of SARS-CoV-2 new variants BA.2.86 and offspring JN.1 in South and Southeast Asia Peer-reviewed

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, W K C P Werawatte, S M Ali Hosseini Rad, Stefan Fernandez, Angkana T Huang, Porames Vatanaprasan, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Ratana Tacharoenmuang, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Bernadina Cynthia, John Jefferson V Besa, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Andrew C Singer, Tanittha Chatsuwan, Sam Trowsdale, Takashi Furukawa, Kazunari Sei, Daisuke Sano, Hitoshi Ishikawa, Kenji Shibuya, Aisha Khatib, Shuichi Abe, Parichart Hongsing

    Journal of Travel Medicine　31　(4)　2024/03/04

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/jtm/taae040 　

    ISSN： 1195-1982

    eISSN： 1708-8305

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    Discover the shifting landscape of SARS-CoV-2 variants from October to December 2023, with JN.1 dominating South and Southeast Asia wastewater samples, increasing from &amp;lt;10% to &amp;gt;90%. Experience the dynamic evolution of viral strains in this period.

  28. Exploring indoor and outdoor dust as a potential tool for detection and monitoring of COVID-19 transmission Peer-reviewed

    Suparinthon Anupong, Sudarat Chadsuthi, Parichart Hongsing, Cameron Hurst, Phatthranit Phattharapornjaroen, Ali Hosseini Rad S.M., Stefan Fernandez, Angkana T. Huang, Porames Vatanaprasan, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Natharin Ngamwongsatit, Vishnu Nayak Badavath, Wanwara Thuptimdang, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Kazuhiko Miyanaga, Longzhu Cui, Asuka Nanbo, Kenji Shibuya, Rosalyn Kupwiwat, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Paul G. Higgins, Anthony Kicic, Andrew C. Singer, Tanittha Chatsuwan, Sam Trowsdale, Shuichi Abe, Hitoshi Ishikawa, Mohan Amarasiri, Charin Modchang, Dhammika Leshan Wannigama

    iScience　27　(3)　109043-109043　2024/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.isci.2024.109043 　

    ISSN： 2589-0042

  29. Recommendations of Key Elements within an Integrated Monitoring Framework of Antimicrobial Resistance for Asian Countries Peer-reviewed

    Ryo Honda, Manish Kumar, Mardalisa, Rongxuan Wang, Muhammad Adnan Sabar, Tushara Chaminda, Kwanrawee Sirikanchana, Prasert Makkeaw, Sulfikar, Feng Ju, Guangming Jiang, Bing Li, Chart Chiemchaisri, Ryota Gomi, Mohan Amarasiri, Henrietta Venter, Masateru Nishiyama, Toru Watanabe, Masaru Ihara, Ikuro Kasuga, Kozo Watanabe, Satoru Suzuki

    Environmental Science &amp; Technology Letters　11　(1)　5-8　2023/12/26

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.estlett.3c00820 　

    ISSN： 2328-8930

    eISSN： 2328-8930

  30. Novel intranasal phage-CaEDTA-ceftazidime/avibactam triple combination therapy demonstrates remarkable efficacy in treating Pseudomonas aeruginosa lung infection Peer-reviewed

    Aye Mya Sithu Shein, Dhammika Leshan Wannigama, Cameron Hurst, Peter N. Monk, Mohan Amarasiri, Vishnu Nayak Badavath, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Wanwara Thuptimdang, Sirirat Luk-in, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Naris Kueakulpattana, Matchima Laowansiri, Tingting Liao, Rosalyn Kupwiwat, Rojrit Rojanathanes, Natharin Ngamwongsatit, Arsa Thammahong, Hitoshi Ishikawa, Daniel Pletzer, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Pattama Wapeesittipan, S.M. Ali Hosseini Rad, Talerngsak Kanjanabuch, Robin James Storer, Kazuhiko Miyanaga, Longzhu Cui, Hiroshi Hamamoto, Paul G. Higgins, Anthony Kicic, Tanittha Chatsuwan, Parichart Hongsing, Shuichi Abe

    Biomedicine &amp; Pharmacotherapy　168　115793-115793　2023/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.biopha.2023.115793 　

    ISSN： 0753-3322

  31. Modeling vaccination strategies with limited early COVID-19 vaccine access in low- and middle-income countries: A case study of Thailand Peer-reviewed

    Suparinthon Anupong, Tanakorn Chantanasaro, Chaiwat Wilasang, Natcha C. Jitsuk, Chayanin Sararat, Kan Sornbundit, Busara Pattanasiri, Dhammika Leshan Wannigama, Mohan Amarasiri, Sudarat Chadsuthi, Charin Modchang

    Infectious Disease Modelling　8　(4)　1177-1189　2023/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.idm.2023.11.003 　

    ISSN： 2468-0427

  32. Tracing the new SARS-CoV-2 variant BA.2.86 in the community through wastewater surveillance in Bangkok, Thailand Peer-reviewed

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Puey Ounjai, Ratana Tacharoenmuang, Naveen Kumar Devanga Ragupathi, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Andrew C Singer, Tanittha Chatsuwan, Sam Trowsdale, Aisha Khatib, Kenji Shibuya, Shuichi Abe, Hitoshi Ishikawa, Parichart Hongsing, Wanwara Thuptiang, Ali Hosseini Rad S M, Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Phitsanuruk Kanthawee

    The Lancet Infectious Diseases　23　(11)　e464-e466　2023/11

    Publisher: Elsevier BV

    DOI： 10.1016/s1473-3099(23)00620-5 　

    ISSN： 1473-3099

  33. Tracing the transmission of mpox through wastewater surveillance in Southeast Asia Peer-reviewed

    Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, Wanwara Thuptimdang, S M Ali Hosseini Rad, Stefan Fernandez, Angkana T Huang, Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Andrew C Singer, Tanittha Chatsuwan, Sam Trowsdale, Richard Siow, Kenji Shibuya, Shuichi Abe, Hitoshi Ishikawa, Parichart Hongsing

    Journal of Travel Medicine　30　(5)　2023/07/18

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/jtm/taad096 　

    ISSN： 1195-1982

    eISSN： 1708-8305

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    Abstract High population density and tourism in Southeast Asia increase the risk of mpox due to frequent interpersonal contacts. Our wastewater surveillance in six Southeast Asian countries revealed positive signals for Monkeypox virus (MPXV) DNA, indicating local transmission. This alerts clinicians and helps allocate resources like testing, vaccines and therapeutics in resource-limited countries.

  34. Ca-EDTA restores the activity of ceftazidime-avibactam or aztreonam against carbapenemase-producing Klebsiella pneumoniae infections Peer-reviewed

    Dhammika Leshan Wannigama, Aye Mya Sithu Shein, Cameron Hurst, Peter N. Monk, Parichart Hongsing, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Pattama Wapeesittipan, Sirirat Luk-in, Sasipen Sae-Joo, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Naris Kueakulpattana, Matchima Laowansiri, Tingting Liao, Rosalyn Kupwiwat, Rojrit Rojanathanes, Natharin Ngamwongsatit, Somkanya Tungsanga, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Vishnu Nayak Badavath, S.M. Ali Hosseini Rad, Talerngsak Kanjanabuch, Nattiya Hirankarn, Robin James Storer, Longzhu Cui, Mohan Amarasiri, Hitoshi Ishikawa, Paul G. Higgins, Stephen M. Stick, Anthony Kicic, Tanittha Chatsuwan, Shuichi Abe

    iScience　26　(7)　107215-107215　2023/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.isci.2023.107215 　

    ISSN： 2589-0042

  35. COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities Peer-reviewed

    Dhammika Leshan Wannigama, Mohan Amarasiri, Parichart Hongsing, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Phatthranit Phattharapornjaroen, Ali Hosseini Rad S. M., Stefan Fernandez, Angkana T. Huang, Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Nattiya Hirankarn, Paul G. Higgins, Anthony Kicic, Andrew C. Singer, Tanittha Chatsuwan, Sam Trowsdale, Shuichi Abe, Alexander D. McLellan, Hitoshi Ishikawa

    iScience　26　(7)　107019-107019　2023/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.isci.2023.107019 　

    ISSN： 2589-0042

  36. Microbial assessment of water, sanitation, and hygiene (WaSH) in temporary and permanent settlements two years after Nepal 2015 earthquake Peer-reviewed

    Sital Uprety, Samendra P. Sherchan, Preeti Narayanan, Bipin Dangol, Marika Maggos, Alex Celmer, Joanna Shisler, Mohan Amarasiri, Daisuke Sano, Thanh H. Nguyen

    Science of The Total Environment　877　162867-162867　2023/06

    Publisher: Elsevier BV

    DOI： 10.1016/j.scitotenv.2023.162867 　

    ISSN： 0048-9697

  37. Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia Peer-reviewed

    Dhammika Leshan Wannigama, Mohan Amarasiri, Parichart Hongsing, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Phatthranit Phattharapornjaroen, Ali Hosseini Rad S.M., Stefan Fernandez, Angkana T. Huang, Naris Kueakulpattana, Chanikan Tanasatitchai, Porames Vatanaprasan, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Nattiya Hirankarn, Paul G. Higgins, Anthony Kicic, Tanittha Chatsuwan, Alexander D. McLellan, Shuichi Abe

    Science of The Total Environment　858　159816-159816　2023/02

    Publisher: Elsevier BV

    DOI： 10.1016/j.scitotenv.2022.159816 　

    ISSN： 0048-9697

  38. Wastewater-based epidemiological surveillance of SARS-CoV-2 reveals silent variant transmission with future outbreaks in low-resource communities

    Mohan Amarasiri

    Respirology　2023

  39. High prevalence of mgrB-mediated colistin resistance among carbapenem-resistant Klebsiella pneumoniae is associated with biofilm formation, and can be overcome by colistin-EDTA combination therapy Peer-reviewed

    Mohan Amarasiri

    Scientific Reports　12　(1)　2022/12

    DOI： 10.1038/S41598-022-17083-5 　

    ISSN： 2045-2322

    eISSN： 2045-2322

  40. Concentration and reduction efficiency of vancomycin-resistant heterotrophic bacteria and vanA and vanB genes in wastewater treatment unit processes Peer-reviewed

    Takashi Furukawa, Tohru Mekata, Mohan Amarasiri, Kazunari Sei

    Journal of Global Antimicrobial Resistance　30　340-347　2022/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.jgar.2022.07.008 　

    ISSN： 2213-7165

  41. Prevalence of antibiotic resistance genes in drinking and environmental water sources of the Kathmandu Valley, Nepal Peer-reviewed

    Mohan Amarasiri, Tsubasa Takezawa, Bikash Malla, Takashi Furukawa, Jeevan B. Sherchand, Eiji Haramoto, Kazunari Sei

    Frontiers in Microbiology　13　2022/08/22

    Publisher: Frontiers Media SA

    DOI： 10.3389/fmicb.2022.894014 　

    eISSN： 1664-302X

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    Antibiotic-resistant bacteria-associated infections are responsible for more than 1.2 million annual deaths worldwide. In low- and middle-income countries (LMICs), the consumption of antibiotics for human and veterinary uses is not regulated effectively. Overused and misused antibiotics can end up in aquatic environments, which may act as a conduit for antibiotic resistance dissemination. However, data on the prevalence of antibiotic resistance determinants in aquatic environments are still limited for LMICs. In this study, we evaluated the prevalence and concentration of antibiotic resistance genes (ARGs) in different drinking and environmental water sources collected from the Kathmandu Valley, Nepal, using droplet digital polymerase chain reaction to understand the current situation of ARG contamination. River water and shallow dug well water sources were the most contaminated with ARGs. Almost all samples containedsul1(94%), andintI1andtet(A) were detected in 83 and 60% of the samples, respectively. Maximum ARG concentration varied between 4.2 log10copies/100 ml formecAand 9.3 log10copies/100 ml forsul1. Significant positive correlations were found between ARGs (r&amp;gt; 0.5,p&amp;lt; 0.01), except formecA, qnrS, andvanA. Assul1andintI1were detected in almost all samples, the presence of these genes in a given sample may need to be considered as background antibiotic resistance in LMICs. Therefore, monitoring of ARGs, such as β-lactam ARGs, quinolone resistance genes, and vancomycin resistance genes, may provide a better picture of the antibiotic resistance determinants in aquatic environments of LMICs.

  42. Inactivation of antibiotic resistant bacteria and their resistance genes in sewage by applying pulsed electric fields Peer-reviewed

    Takashi Furukawa, Takahisa Ueno, Mina Matsumura, Mohan Amarasiri, Kazunari Sei

    Journal of Hazardous Materials　424　127382-127382　2022/02

    Publisher: Elsevier BV

    DOI： 10.1016/j.jhazmat.2021.127382 　

    ISSN： 0304-3894

  43. Summary and Perspectives on Current Disinfection Technologies in Reducing Antibiotic Resistant Bacteria and Their Resistance Genes Peer-reviewed

    TAKASHI FURUKAWA, MOHAN AMARASIRI, TAKAHISA UENO, KAZUNARI SEI

    Japanese Journal of Water Treatment Biology　58　(1)　9-24　2022

    Publisher: Japanese Society of Water Treatment Biology

    DOI： 10.2521/jswtb.58.9 　

    ISSN： 0910-6758

    eISSN： 1881-0438

  44. Multidrug-resistant Neisseria gonorrhoeae infection in heterosexual men with reduced susceptibility to ceftriaxone, first report in Thailand Peer-reviewed

    Naris Kueakulpattana, Dhammika Leshan Wannigama, Sirirat Luk-in, Parichart Hongsing, Cameron Hurst, Vishnu Nayak Badavath, Piroon Jenjaroenpun, Thidathip Wongsurawat, Nipat Teeratakulpisan, Stephen J. Kerr, Shuichi Abe, Phatthranit Phattharapornjaroen, Aye Mya Sithu Shein, Thammakorn Saethang, Naphat Chantaravisoot, Mohan Amarasiri, Paul G. Higgins, Tanittha Chatsuwan

    Scientific Reports　11　(1)　2021/11/04

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-021-00675-y 　

    ISSN： 2045-2322

    eISSN： 2045-2322

  45. Pathogens and disease vectors/hosts monitoring in aquatic environments: Potential of using eDNA/eRNA based approach Peer-reviewed

    Mohan Amarasiri, Takashi Furukawa, Fumiyuki Nakajima, Kazunari Sei

    Science of The Total Environment　796　148810-148810　2021/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.scitotenv.2021.148810 　

    ISSN： 0048-9697

  46. Tracking COVID-19 with wastewater to understand asymptomatic transmission

    Mohan Amarasiri

    INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES　108　296-299　2021/07

    DOI： 10.1016/J.IJID.2021.05.005 　

    ISSN： 1878-3511 1201-9712

  47. Human norovirus disease burden of consuming Crassostrea gigas oysters: A case-study from Japan Peer-reviewed

    Yo Ueki, Mohan Amarasiri, Sayaka Kamio, Akie Sakagami, Hiroshi Ito, Sital Uprety, Arief Nurul Umam, Takayuki Miura, Thanh H. Nguyen, Daisuke Sano

    Food Control　121　107556-107556　2021/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.foodcont.2020.107556 　

    ISSN： 0956-7135

  48. Viral Interference as a Factor of False-Negative in the Infectious Adenovirus Detection Using Integrated Cell Culture-PCR with a BGM Cell Line Peer-reviewed

    Daisuke Sano, Ryosuke Watanabe, Wakana Oishi, Mohan Amarasiri, Masaaki Kitajima, Satoshi Okabe

    Food and Environmental Virology　13　(1)　84-92　2021/01/04

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s12560-020-09453-x 　

    ISSN： 1867-0334

    eISSN： 1867-0342

  49. Updated research agenda for water, sanitation and antimicrobial resistance Peer-reviewed

    Sano, D., Wester, A.L., Schmitt, H., Amarasiri, M., Kirby, A., Medlicott, K., de Roda Husman, A.M.

    Journal of Water and Health　18　(6)　858-866　2020/12/01

    Publisher: {IWA} Publishing

    DOI： 10.2166/wh.2020.033 　

    ISSN： 1996-7829 1477-8920

    eISSN： 1996-7829

  50. The Effect of GD1a Ganglioside-Expressing Bacterial Strains on Murine Norovirus Infectivity Peer-reviewed

    Yifan Zhu, Hiroki Kawai, Satoshi Hashiba, Mohan Amarasiri, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano

    Molecules　25　(18)　4084-4084　2020/09/07

    Publisher: MDPI AG

    DOI： 10.3390/molecules25184084 　

    eISSN： 1420-3049

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    In this study, we investigated the impact of GD1a-expressing bacterial strains on the infectivity of murine norovirus (MNV). Eligible bacterial strains were screened from a sewage sample using flow cytometry, and their genetic sequences of 16S rRNA were determined. The enzyme-linked immunosorbent assay (ELISA) was employed to analyze the binding between bacteria and MNV particles, and the plaque assay was used to assess the effects of GD1a-positive and negative strains on MNV infectivity. The result from ELISA shows that MNV particles are able to bind to both GD1a-positive and negative bacterial strains, but the binding to the GD1a-positive strain is more significant. The infectivity assay result further shows that the MNV infectious titer declined with an increasing concentration of GD1a-positive bacteria. The addition of anti-GD1a antibody in the infectivity assay led to the recovery of the MNV infectious titer, further confirming that the binding between MNV particles and bacterial GD1a ganglioside compromises MNV infectivity. Our findings highlight the role indigenous bacteria may play in the lifecycle of waterborne enteric viruses as well as the potential of exploiting them for virus transmission intervention and water safety improvement.

  51. Identification of novel norovirus polymerase genotypes from pediatric fecal samples collected between the year 1997 and 2000 in Japan Peer-reviewed

    Mohan Amarasiri, Etsuko Utagawa, Daisuke Sano, Kazuhiko Katayama

    Infection, Genetics and Evolution　82　104313-104313　2020/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.meegid.2020.104313 　

    ISSN： 1567-1348

  52. Assessment of microbial risks by characterization of Escherichia coli presence to analyze the public health risks from poor water quality in Nepal Peer-reviewed

    Sital Uprety, Bipin Dangol, Pramina Nakarmi, Isha Dhakal, Samendra P. Sherchan, Joanna L. Shisler, Antarpreet Jutla, Mohan Amarasiri, Daisuke Sano, Thanh H. Nguyen

    International Journal of Hygiene and Environmental Health　226　113484-113484　2020/05

    Publisher: Elsevier BV

    DOI： 10.1016/j.ijheh.2020.113484 　

    ISSN： 1438-4639

  53. Virucidal Efficacy of Olanexidine Gluconate as a Hand Antiseptic Against Human Norovirus Peer-reviewed

    Kaoru Imai, Akifumi Hagi, Yasuhide Inoue, Mohan Amarasiri, Daisuke Sano

    Food and Environmental Virology　12　(2)　180-190　2020/03/02

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s12560-020-09422-4 　

    ISSN： 1867-0334

    eISSN： 1867-0342

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    Abstract Human noroviruses are the major cause of non-bacterial acute gastroenteritis worldwide. Since no therapeutic agent has been proven to prevent human norovirus infection yet, preventive healthcare interventions to block the infection routes play an important role in infection control. One of the possible infection routes of human noroviruses are through contaminated hands, but no hand antiseptics have been proven effective. Olanexidine gluconate is a new biguanide compound that has already been approved for sale as an antiseptic for the surgical field in Japan. A new hand antiseptic was developed using olanexidine gluconate in this study, and its virucidal efficacy against human noroviruses was evaluated using modified RT-qPCR that can account for genome derived from intact viruses using RNase A and photo-reactive intercalators. We tested the virucidal efficacy of five materials; two olanexidine gluconate antiseptics (hand rub formulation and surgical field formulation), two kinds of ethanol solutions at different pH (approx. 3 or 7), and a base component of olanexidine gluconate hand rub formulation against 11 human norovirus genotypes by culture-independent methods. The infectivity of murine norovirus (MNV), a surrogate for human norovirus, was significantly reduced after use of the antiseptics. The olanexidine gluconate hand rub demonstrated the strongest virucidal efficacy against human norovirus among the five tested materials. This study showed that olanexidine gluconate has the potential to become a strong tool for the prevention of human norovirus infection.

  54. Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered Peer-reviewed

    Mohan Amarasiri, Daisuke Sano, Satoru Suzuki

    Critical Reviews in Environmental Science and Technology　50　(19)　1-44　2019/12/03

    Publisher: Informa {UK} Limited

    DOI： 10.1080/10643389.2019.1692611 　

  55. Fecal Source Tracking in A Wastewater Treatment and Reclamation System Using Multiple Waterborne Gastroenteritis Viruses Peer-reviewed

    Zheng Ji, Xiaochang C. Wang, Limei Xu, Chongmiao Zhang, Cheng Rong, Andri Taruna Rachmadi, Mohan Amarasiri, Satoshi Okabe, Naoyuki Funamizu, Daisuke Sano

    Pathogens　8　(4)　170-170　2019/09/30

    Publisher: {MDPI} {AG}

    DOI： 10.3390/pathogens8040170 　

  56. Sign-constrained linear regression for prediction of microbe concentration based on water quality datasets International-journal Peer-reviewed

    Tsuyoshi Kato, Ayano Kobayashi, Wakana Oishi, Syun-Suke Kadoya, Satoshi Okabe, Naoya Ohta, Mohan Amarasiri, Daisuke Sano

    Journal of Water and Health　17　(3)　404-415　2019/06

    DOI： 10.2166/wh.2019.001 　

    More details Close

    This study presents a novel methodology for estimating the concentration of environmental pollutants in water, such as pathogens, based on environmental parameters. The scientific uniqueness of this study is the prevention of excess conformity in the model fitting by applying domain knowledge, which is the accumulated scientific knowledge regarding the correlations between response and explanatory variables. Sign constraints were used to express domain knowledge, and the effect of the sign constraints on the prediction performance using censored datasets was investigated. As a result, we confirmed that sign constraints made prediction more accurate compared to conventional sign-free approaches. The most remarkable technical contribution of this study is the finding that the sign constraints can be incorporated in the estimation of the correlation coefficient in Tobit analysis. We developed effective and numerically stable algorithms for fitting a model to datasets under the sign constraints. This novel algorithm is applicable to a wide variety of the prediction of pollutant contamination level, including the pathogen concentrations in water.

  57. Specific Interactions between Human Norovirus and Environmental Matrices: Effects on the Virus Ecology Peer-reviewed

    Mohan Amarasiri, Daisuke Sano

    Viruses　2019/03/05

    DOI： 10.3390/v11030224 　

  58. Specific interactions of rotavirus HAL1166 with Enterobacter cloacae SENG-6 and their contribution on rotavirus HAL1166 removal Peer-reviewed

    Mohan Amarasiri, Hiroki Kawai, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano

    Water Science and Technology　2019/01

    Publisher: {IWA} Publishing

    DOI： 10.2166/wst.2019.050 　

    ISSN： 0273-1223

  59. Reverse transcription-quantitative PCR assays for genotype-specific detection of human noroviruses in clinical and environmental samples Peer-reviewed

    Mohan Amarasiri, Masaaki Kitajima, Akiho Miyamura, Ricardo Santos, Silvia Monteiro, Takayuki Miura, Shinobu Kazama, Satoshi Okabe, Daisuke Sano

    International Journal of Hygiene and Environmental Health　221　(3)　578-585　2018/04

    Publisher: Elsevier {BV}

    DOI： 10.1016/j.ijheh.2018.02.008 　

  60. Bacteriophage removal efficiency as a validation and operational monitoring tool for virus reduction in wastewater reclamation: Review Peer-reviewed

    Mohan Amarasiri, Masaaki Kitajima, Thanh H. Nguyen, Satoshi Okabe, Daisuke Sano

    Water Research　121　258-269　2017/09

    Publisher: Elsevier {BV}

    DOI： 10.1016/j.watres.2017.05.035 　

  61. Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane Peer-reviewed

    Mohan Amarasiri, Satoshi Hashiba, Takayuki Miura, Toyoko Nakagomi, Osamu Nakagomi, Satoshi Ishii, Satoshi Okabe, Daisuke Sano

    Water Research　95　383-391　2016/05

    Publisher: Elsevier {BV}

    DOI： 10.1016/j.watres.2016.04.018 　

  62. Risk management of viral infectious diseases in wastewater reclamation and reuse: Review Peer-reviewed

    Daisuke Sano, Mohan Amarasiri, Akihiko Hata, Toru Watanabe, Hiroyuki Katayama

    Environment International　91　220-229　2016/05

    Publisher: Elsevier {BV}

    DOI： 10.1016/j.envint.2016.03.001 　

  63. Genome sequence of Enterobacter cloacae strain SENG-6, a bacterium producing histo-blood group antigen-like substances that can bind with human noroviruses Peer-reviewed

    Ishii, S., Amarasiri, M., Hashiba, S., Yang, P., Okabe, S., Sano, D.

    Genome Announcements　4　(4)　e00893-16　2016

    Publisher: American Society for Microbiology

    DOI： 10.1128/genomeA.00893-16 　

  64. Ciprofloxacin resistant bacteria in the waterways surrounding Peradeniya, Sri Lanka

    Mohan Amarasiri

    Sri Lankan Journal of Infectious Diseases

    DOI： 10.4038/sljid.v13i5.8633 　

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Presentations 108

  1. Environmental DNA analysis for the rodents monitoring in the urban drains and environmental samples

    Shiori Kambara, Takashi Furukawa, Mohan Amarasiri, Fumiyuki Nakajima, Noboru Yaguchi, Kazunari Sei

    IWA World Water Congress and Exhibition

  2. Surveillance of antibiotic resistant bacteria and their resistance genes in the wastewater treatment plants in Japan

    Miku Kanazashi, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    IWA World Water Congress and Exhibition

  3. Dynamics of class 1 integrons in wastewater treatment plants for the evaluation of the proliferation and occurrence of antibiotic resistant bacteria

    Kaho Misawa, Tomoya Yamamoto, Mohan Amarasiri, Takashi Furukawa, Shotaro Maehana, Daisuke Sano, Kazunari Sei

    Water and Environment Technology Conference 2024 (WET2024)

  4. Establishment of a method for the detection of rodents by environmental DNA analysis and its application to various urban environmental samples

    Shiori Kambara, Takashi Furukawa, Mohan Amarasiri, Fumiyuki Nakajima, Noboru Yaguchi, Kazunari Sei

    Water and Environment Technology Conference 2024 (WET2024)

  5. Comparative study on the characteristics of antibiotic resistant bacteria and their resistance genes in the wastewater treatment plants in Kanagawa, Japan

    Miku Kanazashi, Mohan Amarasiri, Shotaro Maehana, Takashi Furukawa, Kazunari Sei

    Water and Environment Technology Conference 2024 (WET2024)

  6. Surveillance on dynamic patterns of antibiotic resistant bacteria and multi-drug resistance in Sagami River, Japan

    E. A. C. Priyadarshani, Mizuki Ogino, Mohan Amarasiri, Tatsuru Kamei, Takashi Furukawa, Kazunari Sei

    Environmental Dimension of Antimicrobial Resistance Conference 2024 (EDAR7)

  7. Analysis of antimicrobial resistance genes and plasmid transmissibility in Aeromonas sp. carrying blaIMP-1 isolated from hospital wastewater

    Mako Koyama, Nagi Niida, Ryotaro Eda, Masato Suzuki, Hiroki Izawa, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei, Makoto Kubo, Shotaro Maehana

    Environmental Dimension of Antimicrobial Resistance Conference 2024 (EDAR7)

  8. Dynamics of class 1 integrons in wastewater treatment plants and hospital wastewater

    Kaho Misawa, Tomoya Yamamoto, Mohan Amarasiri, Shotaro Maehana, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    Environmental Dimension of Antimicrobial Resistance Conference 2024 (EDAR7)

  9. intI1遺伝子を指標とした都市下水および病院排水中における薬剤耐性遺伝子の存在実態推定

    三澤香穂, 山本智也, Mohan Amarasiri, 古川隼士, 前花祥太郎, 佐野大輔, 清和成

    第58回日本水環境学会年会

  10. 環境DNA分析による都市の各種排水および環境試料中の鼠族モニタリング

    神原栞, 古川隼士, Mohan Amarasiri, 中島典之, 矢口昇, 清和成

    第58回日本水環境学会年会

  11. 神奈川県下の2ヶ所の下水処理場における下水処理前後の薬剤耐性菌と耐性遺伝子の比較調査

    金刺未来, Mohan Amarasiri, 古川隼士, 前花祥太郎, 清和成

    第58回日本水環境学会年会

  12. パルス電界応用技術によるバクテリオファージの不活化効果の検証

    諸石涼羽, 木下樺子, 古川隼士, 上野崇寿, Mohan Amarasiri, 清和成

    第58回日本水環境学会年会

  13. パルス電界印加技術によるエアロゾル感染性ウイルスの不活化効果の検証

    諸石涼羽, 木下樺子, 古川隼士, 上野崇寿, Mohan Amarasiri, 清和成

    第60回環境工学研究フォーラム

  14. パルス電界を用いた水耕栽培養液中の植物病原体不活化効果の検証

    古川隼士, 野崎正洋, 上野崇寿, Mohan Amarasiri, 清和成

    第60回環境工学研究フォーラム

  15. intI1遺伝子を指標とした下水処理場での薬剤耐性遺伝子削減効果の評価

    三澤香穂, 山本智也, Mohan Amarasiri, 古川隼士, 前花祥太郎, 佐野大輔, 清和成

    第59回日本水処理生物学会大会

  16. 環境DNA解析による鼠族検出法の確立と都市の各種排水への適用

    神原栞, 古川隼士, Mohan Amarasiri, 矢口昇, 中島典之, 清和成

    第59回日本水処理生物学会大会

  17. パルス電界応用技術による空気感染性ウイルスの不活化効果の検証

    諸石涼羽, 木下樺子, 上野崇寿, 古川隼士, Mohan Amarasiri, 清和成

    第59回日本水処理生物学会大会

  18. 環境DNAおよび環境RNA定量解析によるマンソン住血吸虫中間宿主貝検出の時空間的感度評価

    竹澤翼, Mohan Amarasiri, 古川隼士, 清和成

    第59回日本水処理生物学会大会

  19. Studying the temporal and spatial variations of antibiotic resistant bacteria in Sagami River

    E.A.C. Priyadarshani, Mizuki Ogino, Tatsuru Kamei, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    The 59th Japanese Society for Water Treatment Biology Conference

  20. 神奈川県下の2ヶ所の下水処理場における流入水および放流水中の薬剤耐性菌と耐性遺伝子の特徴

    金刺未来, Mohan Amarasiri, 古川隼士, 前花祥太郎, 清和成

    第59回日本水処理生物学会大会

  21. Establishment of a simple rodent monitoring method based on environmental DNA analysis and its application for the rodents monitoring in the drains

    Shiori Kambara, Takashi Furukawa, Mohan Amarasiri, Fumiyuki Nakajima, Kazunari Sei

    The 9th IWA-ASPIRE Conference & Exhibition

  22. Surveillance of antibiotic resistant bacteria and their resistance genes in the wastewater treatment plants in Kanagawa

    Miku Kanazashi, Mohan Amarasiri, Shotaro Maehana, Takashi Furukawa, Kazunari Sei

    The 9th IWA-ASPIRE Conference & Exhibition

  23. Recovery and inactivation of pathogenic bacteria in aerosols by applying pulsed electric field

    Suzuha Moroishi, Takashi Furukawa, Takahisa Ueno, Mohan Amarasiri, Kazunari Sei

    The 9th IWA-ASPIRE Conference & Exhibition

  24. 水環境における薬剤耐性菌の発生源と挙動解明の試み～水環境中の薬剤耐性菌はどこからやってくるのか？

    清和成, Mohan Amarasiri, 古川隼士

    第26回日本水環境学会シンポジウム

  25. Verification and inactivation effect of aerosol-infectious virus by pulsed electric field application technology

    Suzuha Moroishi, Kako Kinoshita, Takashi Furukawa, Takahisa Ueno, Mohan Amarasiri, Kazunari Sei

    15th International Joint Workshop on Advanced Engineering Technology for Environment and Energy

  26. Establishment of a simple rodent monitoring method based on environmental DNA analysis and its application for the rodent monitoring in drains

    Shiori Kambara, Takashi Furukawa, Mohan Amarasiri, Fumiyuki Nakajima, Kazunari Sei

    15th International Joint Workshop on Advanced Engineering Technology for Environment and Energy

  27. Antibiotic resistance profile and the prevalence of six major β-lactamase gene subtypes in Sagami River

    E.A.C. Priyadarshani, Mizuki Ogino, Mohan Amarasiri, Tatsuru Kamei, Takashi Furukawa, Kazunari Sei

    15th International Joint Workshop on Advanced Engineering Technology for Environment and Energy

  28. Investigation of antimicrobial resistant bacteria and their resistance profiles in Sagami River basin

    E.A.C. Priyadarshani, Mizuki Ogino, Mohan Amarasiri, Tatsuru Kamei, Takashi Furukawa, Kazunari Sei

    Water and Environment Technology Conference 2023 (WET2023)

  29. Establishment of a rodent detection system by Environmental DNA analysis and its application to the urban drains

    Shiori Kambara, Takashi Furukawa, Mohan Amarasiri, Fumiyuki Nakajima, Kazunari Sei

    Water and Environment Technology Conference 2023 (WET2023)

  30. Surveillance of antibiotic resistant bacteria and their resistance genes in the wastewater treatment plants in Kanagawa, Japan

    Miku Kanazashi, Mohan Amarasiri, Shotaro Maehana, Takashi Furukawa, Kazunari Sei

    Water and Environment Technology Conference 2023 (WET2023)

  31. Prevalence of antibiotic resistance genes in aquatic samples and their reduction after a WaSH intervention

    Mohan Amarasiri, Sital Uprety, Chihiro Konuma, Takashi Furukawa, Kazunari Sei, Helen Nguyen, Daisuke Sano

    The 21st IWA Symposium on Health-Related Water Microbiology

  32. Temporal dynamics of class 1 integrons in hospital wastewater

    Mohan Amarasiri, Tomoya Yamamoto, Shotaro Maehana, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    The 21st IWA Symposium on Health-Related Water Microbiology

  33. 病院排水より分離された広域β-ラクタムおよびチゲサイクリン耐性菌の存在実態と耐性遺伝子解析

    石村菜穂子, 江田諒太郎, 伊澤紘輝, Mohan Amarasiri, 古川隼士, 清和成, 久保誠, 前花祥太郎

    第57回日本水環境学会年会

  34. 病院排水中における薬剤耐性遺伝子の水平伝播に影響を及ぼすインテグロン動態の解明

    山本智也, Mohan Amarasiri, 前花祥太郎, 古川隼士, 佐野大輔, 清和成

    第57回日本水環境学会年会

  35. パルス高電界を用いた水耕栽培養液の消毒効果の評価

    野崎正洋, 古川隼士, 上野崇寿, Mohan Amarasiri, 清和成

    第57回日本水環境学会年会

  36. 環境DNA／RNA解析による鼠族検出系の確立と病院排水管からの鼠族検出への適用

    神原栞, 古川隼士, Mohan Amarasiri, 中島典之, 清和成

    第57回日本水環境学会年会

  37. 神奈川県下の２ヶ所の下水処理場における流入水および放流水中の薬剤耐性菌と耐性遺伝子の存在実態調査

    金刺未来, Mohan Amarasiri, 古川隼士, 前花祥太郎, 清和成

    第57回日本水環境学会年会

  38. パルス高電界によるエアロゾル中の細菌の回収・不活化

    諸石涼羽, 古川隼士, 上野崇寿, Mohan Amarasiri, 清和成

    第57回日本水環境学会年会

  39. 過酢酸、過ギ酸による下水二次処理水中の細胞外薬剤耐性遺伝子の不活化評価

    小林大起, Mohan Amarasiri, 古川隼士, 佐野大輔, 清和成

    第57回日本水環境学会年会

  40. 相模川における薬剤耐性菌と薬剤耐性遺伝子の存在実態と耐性プロファイルの調査

    荻野瑞葵, 金刺未来, 亀井樹, Mohan Amarasiri, 古川隼士, 清和成

    第57回日本水環境学会年会

  41. 結合塩素による薬剤耐性遺伝子の接合伝達効率への影響評価

    森山桃子, Mohan Amarasiri, 鈴木聡, 佐野大輔

    第57回日本水環境学会年会

  42. Detection of antibiotic resistance genes (ARGs) in drinking and environmental water sources of the Kathmandu Valley, Nepal using droplet digital PCR

    Tsubasa Takezawa, Mohan Amarasiri, Bikash Malla, Takashi Furukawa, Jeevan Sherchand, Eiji Haramoto, Kazunari Sei

    14th International Joint Workshop on Advanced Engineering Technology for Environment and Energy　2022/10/15

  43. Inactivation of extracellular antibiotic resistance genes using peracetic acid (PAA) and performic acid (PFA)

    Hiroki Kobayashi, Mohan Amarasiri, Ryuji Ampo, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    14th International Joint Workshop on Advanced Engineering Technology for Environment and Energy　2022/10/15

  44. Monitoring and characterization of antibiotic resistant bacteria in Sagami River for the identification of their origins

    Mizuki Ogino, Miku Kanazashi, Shiori Kambara, Tatsuru Kamei, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    14th International Joint Workshop on Advanced Engineering Technology for Environment and Energy　2022/10/15

  45. Identification of possible origins of ARB in river environments

    Mizuki Ogino, Miku Kanazashi, Shiori Kambara, Tatsuru Kamei, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    6th International Symposium on the Environmental Dimension of Antibiotic Resistance (EDAR6)

  46. Prevalence of antibiotic resistance genes in drinking and environmental water sources of the Kathmandu Valley, Nepal

    Tsubasa Takezawa, Mohan Amarasiri, Bikash Malla, Takashi Furukawa, Jeevan Sherchand, Eiji Haramoto, Kazunari Sei

    IWA World Water Congress & Exhibition

  47. Disinfection of extracellular antibiotic resistance genes using peracetic acid (PAA) and performic acid (PFA)

    Hiroki Kobayashi, Mohan Amarasiri, Ryuji Ampo, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    IWA World Water Congress & Exhibition

  48. Disinfection of extracellular antimicrobial resistant genes (ARGs) using free chlorine (Cl), peracetic acid (PAA), and performed acid (PFA)

    Hiroki Kobayashi, Mohan Amarasiri, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    Water and Environment Technology Conference 2022 (WET2022)

  49. Abundance of antimicrobial resistant bacteria and their resistance profiles in Sagami River basin

    Mizuki Ogino, Miku Kanazashi, Shiori Kambara, Tatsuru Kamei, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    Water and Environment Technology Conference 2022 (WET2022)

  50. Investigation of antibiotic resistance genes in river water and household water in the Kathmandu Valley, Nepal

    Tsubasa Takezawa, Mohan Amarasiri, Bikash Malla, Takashi Furukawa, Eiji Haramoto, Kazunari Sei

    Water and Environment Technology Conference 2022 (WET2022)

  51. Capture and inactivation of fecal indicator bacteria in aerosols by applying pulsed electric field

    Suzuha Moroishi, Takashi Furukawa, Mohan Amarasiri, Takahisa Ueno, Kazunari Sei

    Water and Environment Technology Conference 2022 (WET2022)

  52. ネパール・カトマンズ盆地の河川水・生活用水の薬剤耐性遺伝子汚染の実態調査

    竹澤翼, Mohan Amarasiri, 古川隼士, Bikash Malla, 原本英司, 清和成

    第56回日本水環境学会年会

  53. 水環境条件下における薬剤耐性遺伝子の接合伝達効率に影響を与える環境因子に関する研究

    森山桃子, Amarasiri Mohan, 鈴木聡, 佐野大輔

    第56回日本水環境学会年会

  54. 簡易消毒による環境中の細胞外薬剤耐性遺伝子への物理的な消毒効果及び形質転換効率への影響の比較評価

    小林大起, 安保龍二, Mohan Amarasiri, 古川隼士, 佐野大輔, 清和成

    第56回日本水環境学会年会

  55. PCR screening reveals the existence of quinolone resistance gens in anthropogenically affected waterways of Badulla and Kandy Districts

    M.H.M.I.M. Gunawardane, C.D. Gamage, S. Rajapakse, G.M.T.M.Herath, A.K.U.I. Karunadasa, E.W.M.A.Ekanayake, Takashi Furukawa, Mohan Amarasiri, Kazunari Sei

    Postgraduate Institute of Science Research Congress

  56. Abundance of antimicrobial resistant bacteria and their antimicrobial resistant profiles in hospital sewage

    Jo Yonemoto, Mizuki Ogino, Nagi Niida, Ryotaro Eda, Shotaro Maehana, Mohan Amarasiri, Takashi Furukawa, Hidero Kitasato, Kazunari Sei

    Water and Environment Technology conference 2021 (WET2021)

  57. Abundance of antimicrobial resistant bacteria and their resistance profiles in Sagami River basin

    Mizuki Ogino, Yukiko Kobayashi, Tatsuru Kamei, Mohan Amarasiri, Takashi Furukawa, Kazunari Sei

    Water and Environment Technology conference 2021 (WET2021)

  58. Inactivation efficiency assessment of antimicrobial resistance genes (ARGs) existing as extracellular DNA using simple disinfection methods

    Hiroki Kobayashi, Mohan Amarasiri, Takashi Furukawa, Daisuke Sano, Kazunari Sei

    Water and Environment Technology conference 2021 (WET2021)

  59. 病院排水中の薬剤耐性菌の存在実態と耐性プロファイルの調査

    米本譲, 荻野瑞葵, 小林由紀子, 新井田凪, 江田諒太郎, 前花祥太郎, Amarasiri Mohan, 古川隼士, 北里英郎, 清和成

    第55回日本水環境学会年会

  60. 環境DNAとして存在する薬剤耐性遺伝子への簡易消毒による不活化効果の評価

    小林大起, Amarasiri Mohan, 古川隼士, 佐野大輔, 清和成

    第55回日本水環境学会年会

  61. 相模川における薬剤耐性菌の存在実態と耐性プロファイルの調査

    荻野瑞葵, 米本譲, 小林由紀子, 亀井樹, Amarasiri Mohan, 古川隼士, 清和成

    第55回日本水環境学会年会

  62. 水環境条件下における薬剤耐性遺伝子の接合伝達効率に関する研究

    森山桃子, Mohan Amarasiri, 鈴木聡, 佐野大輔

    第55回日本水環境学会年会

  63. パルス電界印加技術を用いた薬剤耐性菌および耐性遺伝子の不活化効果の検証

    松村美那, 古川隼士, 上野崇寿, Amarasiri Mohan, 清和成

    第55回日本水環境学会年会

  64. 環境DNA/環境RNA解析による衛生動物検出の時間的感度評価

    須江渚, 大知鼓太郎, 飯塚洸平, 前田倖, Amarasiri Mohan, 古川隼士, 中島典之, 清和成

    第55回日本水環境学会年会

  65. 薬剤耐性遺伝子による生活環境の汚染に対するWASH介入効果の評価

    小沼千紘, Mohan Amarasiri, Sital Uprety, Thanh Nguyen, 鈴木聡, 佐野大輔

    第55回日本水環境学会年会

  66. パルス電界印加技術を応用した薬剤耐性菌および耐性遺伝子の削減効果

    松村美那, 古川隼士, 上野崇寿, Amarasiri Mohan, 清和成

    第57回環境工学研究フォーラム

  67. 環境RNA解析による衛生動物の高感度検出手法の開発および時間的感度の評価

    須江渚, 古川隼士, 中島典之, Amarasiri Mohan, 清和成

    第57回環境工学研究フォーラム

  68. Evaluation of microbial health risks caused by the usage of river water for a decentralized drinking water system

    Arief Umam, Syun-suke Kadoya, Mohan Amarasiri, Daisuke Sano

    The 23rd Symposium of Japanese Society for Water Environment

  69. 自然条件下における水中腸管系ウイルス自然死滅モデルの構築

    加藤郁生, モハン アマラシリ, 佐野大輔

    第54回日本水環境学会年会

  70. 水環境サンプルからの形質転換可能な細胞外薬剤耐性遺伝子の回収方法の確立

    小沼千紘, モハン アマラシリ, 鈴木聡, 佐野大輔

    第54回日本水環境学会年会

  71. Histo-blood group antigen-like substances bearing bacteria are prevalent in wastewater Invited

    Mohan Amarasiri

    The22nd U.S.-Japan Cooperative Medical Sciences Program (USJCMSP), Viral Diseases Panel Meeting

  72. Antibiotic-induced Changes in Enterobacter cloacae SENG-6 Extracellular Polymeric Substances

    Mohan Amarasiri, Hui Cheng, Yu-You Li, Daisuke Sano

    The 8th IWA-Microbial Ecology and Water Engineering Specialist Conference

  73. Microbial Assessment of Water, Sanitation and Hygiene (WASH) Two Years After Nepal 2015 Earthquake

    Sital Uprety, Mohan Amarasiri, Bipin Dangol, Daisuke Sano, Helen Nguyen

    The 8th IWA-Microbial Ecology and Water Engineering Specialist Conference

  74. Human health risk of Legionella pneumophila infection caused by the groundwater usage in a decentralized drinking water system

    Arief Umam, Mohan Amarasiri, Daisuke Sano

    The 7th International Symposium on Water Environment Systems　2019/11/15

  75. Water, Sanitation and Hygiene (WaSH) assessments two years after Nepal 2015 Earthquake

    Sital Uprety, Mohan Amarasiri, Bipin Dangol, Daisuke Sano, Helen Nguyen

    World Bosai Forum 2019

  76. Quantification of human health risks caused by antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Future research directions Invited

    Mohan Amarasiri

    The 6th Environmental Technology and Management Conference

  77. Impact of Water, Sanitation, and Hygiene (WASH) interventions on the bacterial pathogen load in households in rural Nepal

    Sital Uprety, Mohan Amarasiri, Bipin Dangol, Daisuke Sano, Helen Nguyen

    IWA Symposium on Health-Related Water Microbiology

  78. Extracellular antibiotic resistance gene decay by free chlorine treatment International-presentation

    Mohan Amarasiri, Chihiro Konuma, Daisuke Sano

    IWA Health Related Water Microbiology

  79. Quantitative microbial risk assessment for users of a decentralized drinking water system

    Arief Umam, Mohan Amarasiri, Daisuke Sano

    The 6th Symposium for Environmental Water Quality Engineering　2019/06/22

  80. 細胞外薬剤耐性遺伝子の発現に与える遊離塩素の影響

    小沼千紘, 門屋俊祐, モハン アマラシリ, 佐野大輔

    第６回環境水質工学シンポジウム　2019/06/22

  81. 水中病原ウイルスの自然死滅モデルパラメータ推定式の構築

    加藤郁生, モハン アマラシリ, 佐野大輔

    第53回日本水環境学会年会

  82. 細胞外薬剤耐性遺伝子の遊離塩素による消毒効果の評価

    小沼千紘, モハン アマラシリ, 門屋俊祐, 佐野大輔

    平成30年度土木学会東北支部技術研究発表会　2019/03/02

  83. Identification of bacterial genes for producing norovirus-binding glycoconjugate International-presentation

    Amarasiri, M, Yang, P.Y, Kitajima, M, Kasahara, Y, Okabe, S, Sano, D

    6th Food and Environmental Virology Conference　2018/10/07

  84. Contribution of specific interactions between human enteric viruses and wastewater solids on virus removal International-presentation

    Amarasiri, M, Kawai, H, Kitajima, M, Okabe, S, Sano, D

    IWA world water congress and exhibition　2018/09/16

  85. Screening of bacterial genes responsible for producing norovirus-binding glycoconjugate International-presentation

    Amarasiri, M, Yang, P.Y, Kitajima, M, Kasahara, Y, Okabe, S, Sano, D

    Water and environment technology (WET) conference　2018/07/14

  86. Genotype-dependent behaviors of human noroviruses in aquatic environments International-presentation Invited

    Amarasiri, M

    Symposium on risk reduction of infectious diseases from different perspectives　2018/03/20

  87. 腸内細菌の組織血液型決定抗原様物質分泌関連遺伝子に関する研究

    小島友祐, モハン・アマラシリ, 佐野大輔

    日本大学郡山キャンパス　2018/03/03

  88. 病原ウイルスの自然死滅モデルに関する研究

    加藤郁生, 稲葉愛美, モハン・アマラシリ, 佐野大輔

    日本大学郡山キャンパス　2018/03/03

  89. Human enteric virus removal from wastewater: Design and operational monitoring of multiple-barrier system and virus removal efficiency improvement using specific interaction International-presentation

    Amarasiri, M

    20th Symposium of Japanese Society for Water Environment　2017/09/26

  90. Histo-blood group antigen (HBGA) positive bacteria in the mixed liquor reduces the human rotavirus concentration in membrane filtration effluent International-presentation

    Amarasiri, M, Kawai, H, Kitajima, M, Okabe, S, Sano, D

    Water and environment technology (WET) conference　2017/07/22

  91. Removal of rotavirus by microfiltration in the presence of HBGA-positive bacteria International-presentation

    Amarasiri, M, Kawai, H, Kitajima, M, Okabe, S, Sano, D

    4th Symposium for Environmental Water Quality Engineering　2017/06/17

  92. 環境水中からのノロウイルス吸着性大腸菌の単離と吸着因子同定に関する研究

    羽柴聡, AMARASIRI Mohan, 原本英司, 北島正章, 岡部聡, 佐野大輔

    第51回日本水環境学会年会

  93. Effects of wastewater solids-virus interactions on virus removal and survival in wastewater International-presentation Invited

    Amarasiri, M

    Japan-YWP International forum on water treatment technology　2017/02/20

  94. Human glycan-positive bacteria as an adsorbent for norovirus International-presentation

    Amarasiri, M, Hashiba, S, Okabe, S, Miyoshi, T, Tanaka, T, Sano, D

    64th Annual Meeting of The Japanese Society for Virology　2016/10/23

  95. Diversity of histo-blood group antigen-positive enteric bacteria as a specific adsorbent for human noroviruses

    Daisuke Sano, Mohan Amarasiri, Satoshi Hashiba, Takatomo Ohta, Takayuki Miura, Toyoko Nakagomi, Osamu Nakagomi, Satoshi Okabe

    The 6th International Calicivirus Conference

  96. A systematic review and meta-analysis on the virus removal efficiencies of wastewater treatment plant unit processes International-presentation

    Amarasiri, M, Sano, D, Hata, A, Watanabe, T, Katayama, H

    5th Food and Environmental Virology Conference　2016/09/13

  97. Genotype-dependent norovirus removal by microfiltration: Influence of bacterial histo-blood group antigens International-presentation

    Amarasiri, M, Hashiba, S, Okabe, S, Sano, D

    3rd Symposium for Environmental Water Quality Engineering　2016/05/21

  98. Effect of localization of HBGA-like substances on membrane separation on norovirus International-presentation

    Amarasiri, M, Hashiba, S, Okabe, S, Sano, D

    3rd International Life-Science Symposium　2015/11/26

  99. Diverse enteric bacteria can excrete histo-blood group antigens as adsorbents for human gastroenteritis viruses International-presentation

    Sano, D, Ohta, T, Amarasiri, M, Nakagomi, T, Miura, T, Nakagomi, O, Okabe, S

    63rd Annual Meeting of the Japanese Society for Virology　2015/11/22

  100. Diversity of histo-blood group antigen-positive enteric bacteria in water: possible environmental vehicle for human norovirus International-presentation

    Sano, D, Ohta, T, Amarasiri, M, Miura, T, Nakagomi, T, Nakagomi, O, Okabe, S

    18th International symposium on health-related water microbiology　2015/09/13

  101. Microfiltration of human noroviruses with blood-type active enteric bacteria

    Amarasiri, M, Yoshimura, T, Okabe, S, Sano, D

    22nd Public Health Engineering Symposium　2014/11/21

  102. Genotype-dependent membrane separation of human noroviruses with bacterial histo-blood group antigen (HBGA)-like substances International-presentation

    Amarasiri, M, Yoshimura, T, Nakagomi, T, Nakagomi, O, Okabe, S, Sano, D

    4th Food and Environmental Virology Conference　2014/09/02

  103. Development of a screening assay of norovirus-binding enteric bacteria bearing histo-blood group antigen-like substances International-presentation

    Sano, D, Amarasiri, M, Yoshimura, T, Nakagomi, T, Nakagomi, O, Okabe, S

    International Union of Microbiological Societies　2014/07/27

  104. Membrane separation of human noroviruses in combination with enteric bacteria bearing histo-blood group antigen (HBGA)-like substances International-presentation

    Amarasiri, M, Yoshimura, T, Okabe, S, Sano, D

    IWA specialist conference on advances in particle science and separation

  105. Norovirus removal with microfiltration in the presence of HBGA-like substances-bearing bacteria: A new approach to improve removal efficiency International-presentation

    Amarasiri, M, Yoshimura, T, Nakagomi, T, Nakagomi, O, Okabe, S, Sano, D

    The 1st Symposium for Environmental Water Quality Engineering　2014/05/09

  106. Human enteric bacteria-receptors of human norovirus and implications in microfiltration

    Amarasiri, M, Sano, D, Yoshimura, T, Ishizaki, S, Nakagomi, T, Nakagomi, O, Okabe, S

    21st Public Health Engineering Symposium　2013/12/04

  107. Microfiltration of human noroviruses: Effects of the presence of human enteric bacteria on virus rejection International-presentation

    Amarasiri, M, Sano, D, Yoshimura, T, Ishizaki, S, Nakagomi, T, Nakagomi, O, Okabe, S

    Water and environment technology (WET) conference　2013/06/15

  108. Risk to Groundwater from Improper Toilet Waste Disposals

    Mohan Amarasiri, Gemunu Herath, Tharanga Jayathilaka, Chatura Weerasinghe

    International Symposium on Water Quality and Human Health: Challenges Ahead

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Research Projects 8

  1. Development of a technical strategy for the source control of antibiotic resistant bacteria and antibiotic resistance genes based on one-health approach

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Kitasato University

    2023/04/01 - 2026/03/31

  2. 薬剤耐性菌の排出負荷低減に資する高電圧パルス電界を応用した下水消毒システムの構築

    古川 隼士, 上野 崇寿, 前花 祥太郎, Amarasiri Mohan, 清 和成

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 北里大学

    2023/04/01 - 2026/03/31

  3. スリランカにおける下水水質情報に基づいた下水管路劣化予測システムの開発

    佐野 大輔, 水谷 大二郎, Amarasiri Mohan

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 国際共同研究加速基金(国際共同研究強化(B))

    Institution: 東北大学

    2021/10/07 - 2024/03/31

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    本研究は、スリランカにおいて、微生物の起源を同定する手法であるMicrobial Source Tracking（MST）の手法を応用し、下水管の破損部分から混入する土壌由来の微生物の下水中に占める割合を空間的・時間的に把握することで、下水管破損箇所を推定し、かつ下水管劣化速度の予測に適用するための新規手法を開発することを目指すものである。研究初年度である令和3年度においては、まずスリランカ側カウンターパート（チャンディカ・ガマゲ（ペラデニヤ大学医学部・講師）、及びチャミンダ・サマラスーリヤ（モラトゥワ大学地球資源工学部・講師））とのオンライン打ち合わせを通じ、スリランカ国内における調査地点の確定を行った。希望する調査地点で下水採取を行うために、所轄官庁向けの要望書を作成・提出したところである。さらに、本研究で想定している手法が適用可能であることを確認するために、日本国内で未処理下水を採取し、総DNAを抽出して、細菌の分類に用いられる16s rRNA遺伝子配列の取得を行った。その結果、下水に特徴的な細菌種の16s rRNA遺伝子配列が確実に得られることを確認することに成功し、スリランカで得られたサンプルに適用する準備が整えられた。

  4. Development of temporally sensitive detection method for vectors of infectious diseases based on environmental RNA

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Pioneering)

    Institution: Kitasato University

    2020/07/30 - 2023/03/31

  5. Elucidation of source and environmental behavior of antibiotic-resistant bacteria and resistance genes by microbial source tracking

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Kitasato University

    2020/04/01 - 2023/03/31

  6. Research on the establishment of a new disinfection technique applying the pulsed electric field and the determination of its disinfection mechanism

    Furukawa Takashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Kitasato University

    2019/04/01 - 2022/03/31

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    We evaluated the suitability of pulsed electric field (PEF) technology as a new disinfection option in the sewage treatment plants (STPs) that can inactivate antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). It was shown that PEF applied disinfection could inactivate not only vancomycin-resistant enterococci (VRE), but also vanA resistance gene. Cultivable VRE could be effectively inactivated by PEF applied disinfection, and were reduced to below the detection limit (log reduction value of VRE > 5 log). Although the vanA also showed a reduction of more than 4 log, it remained in the order of 105 copies/mL, suggesting that ARGs are more difficult to be inactivated than ARB in PEF applied disinfection. Among parameters in each applying condition verified in this study, the initial voltage was found to be the most important for inactivation of ARB and ARGs.

  7. Development of alternative disinfection methods to inactivate extracellular antibiotic resistance genes in aquatic environments

    Mohan Amarasiri

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    Category: Grant-in-Aid for Early-Career Scientists

    Institution: Tohoku University

    2020/04 - 2022/03

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    We evaluated the inactivation efficiency of extracellular antibiotic resistance genes (ARGs) by free chlorine (Cl), peracetic acid (PAA), and performic acid (PFA) using plasmid pUC19, which carries ampicillin resistant gene, as a model. Reduction of viable extracellular ARGs was evaluated by calculating the reduction of transformation frequency of pUC19 exposed to disinfectants with Escherichia coli competent cells. Free chlorine showed high and time-dependent disinfection effect even at 1 ppm concentration. For PAA and PFA, the inactivation efficiency increased with initial concentration and time dependency was almost negligible. A 2.3 and 2.9 log reductions of viable pUC19 was observed by PAA and PFA. PAA and PFA has not shown to generate harmful disinfection by products and therefore can be considered as alternative disinfectants usable in decentralized facilities to minimize the spread of antibiotic resistance.

  8. Effects of antibiotics in wastewater on the removal of human enteric viruses by specific interaction between virus and wastewater solids Competitive

    Mohan Amarasiri

    Offer Organization: Japanese Society for the Promotion of Science

    System: Kakenhi

    Category: Grant-in-Aid for Early-Career Scientists

    Institution: Tohoku University

    2018/04 - 2020/03

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    We evaluated the MIC of amikacin and chloramphenicol to Enterobacter cloacae using E-test. After that we cultivated the bacteria under subinhibitory level antibiotics concentrations. We extracted the SMP, LB-EPS and TB-EPS production. Carbohydrate and protein concentrations of extracted fractions were measured. We observed changes in the EPS structure by Excitation Emission Matrix fluorescence spectroscopy. After that we conducted a microfiltration experiment of rotavirus HAL1166 in the presence of E.cloacae grown under different antibiotic concentrations. A review paper titled "Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered" is published in Critical Reviews in Environmental Science and Technology journal.

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Teaching Experience 12

  1. Introduction of Environment Health　Kitasato University

  2. Introduction to Sustainability Science and Technology　Kitasato University

  3. Eco-Environmental Engineering　Kitasato University

  4. Environmental Hygiene　Kitasato University

  5. Microbiology　Kitasato University

  6. Laboratory in Environmental Hygiene and Engineering　Kitasato University

  7. Eco-Environmental Engineering　Kitasato University

  8. Laboratory in Microbiology　Kitasato University

  9. Laboratory in Instrumental Analysis　Kitasato University

  10. Tutorial of Health Science　Kitasato University

  11. Exercises in Water and Environmental Studies II　Tohoku University

  12. Exercises in Civil Engineering and Architecture　Tohoku University

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