研究者詳細

顔写真

タカヤマ　ユウキ
高山　裕貴
Yuki Takayama
所属
国際放射光イノベーション・スマート研究センター　展開研究部門　農業・食品スマートラボ
職名
准教授
学位

  • 博士（理学）（慶應義塾大学）

  • 修士（理学）（慶應義塾大学）

e-Rad 研究者番号
40710132

経歴 4

  • 2022年10月 ～ 継続中
    東北大学　国際放射光イノベーション・スマート研究センター 展開研究部門　准教授

  • 2021年4月 ～ 2022年9月
    兵庫県立大学　大学院 理学研究科　助教

  • 2016年4月 ～ 2021年3月
    兵庫県立大学　大学院 物質理学研究科　助教

  • 2013年4月 ～ 2016年3月
    理化学研究所　放射光科学総合研究センター　基礎科学特別研究員

学歴 3

  • 慶應義塾大学　大学院 理工学研究科　基礎理工学専攻 後期博士課程

    2010年4月 ～ 2013年3月

  • 慶應義塾大学　大学院 理工学研究科　基礎理工学専攻 修士課程

    2008年4月 ～ 2010年3月

  • 慶應義塾大学　理工学部　物理学科

    2004年4月 ～ 2008年3月

所属学協会 4

  • 農芸化学会

    2024年5月 ～ 継続中

  • 日本結晶学会

  • 日本生物物理学会

  • 日本放射光学会

研究キーワード 4

  • タイコグラフィー

  • 生物物理学

  • X線顕微鏡

  • コヒーレント回折イメージング

研究分野 1

  • 自然科学一般 / 生物物理、化学物理、ソフトマターの物理 /

受賞 8

  1. 第20回SPring-8産業利用報告会 ポスター発表の部 優秀発表賞

    2023年11月　SPring-8利用推進協議会　SAXSイメージングモデルを用いたシミュレーションによる手延べ素麺の食感解析

  2. 日本結晶学会賞進歩賞

    2023年7月　一般社団法人 日本結晶学会　マルチショット・コヒーレントX線回折イメージングの開発と実用化

  3. 2023年春期講演大会 第40回ポスターセッション 優秀ポスター賞

    2023年3月　公益社団法人日本金属学会　in situ 2D XAFS と機械学習による材料腐食メカニズム推定

  4. 第19回SPring-8産業利用報告会 ポスター発表の部 優秀発表賞

    2022年11月　SPring-8利用推進協議会　手延べ製法の条件と手延べ素麺の美味しさ評価

  5. 優秀論文発表賞A

    2022年2月　一般社団法人 電気学会　コヒーレントX線を用いたレンズレス時空間階層イメージング法の開発

  6. SPRUC 2021 Young Scientist Award

    2021年7月　SPring-8ユーザー協同体　コヒーレントX線を用いたレンズレス時空間階層イメージング法の開発

  7. 第16回SPring-8産業利用報告会 口頭発表の部 優秀発表賞

    2019年11月　SPring-8利用推進協議会　真空フライによるケールの高機能化と構造・機能性・おいしさ評価

  8. 理工学研究科 藤原賞

    2013年3月　慶應義塾大学　湿度制御技術開発を軸とした細胞内空間階層構造のX線回折イメージング

︎全件表示 ︎最初の5件までを表示

論文 60

  1. Three-dimensional structure of printer toner visualized using cryogenic X-ray diffraction imaging tomography 査読有り

    Kosei Harada, Yuki Takayama, Masayoshi Nakasako

    Journal of Synchrotron Radiation　32　(6)　1491-1502　2025年10月16日

    出版者・発行元： International Union of Crystallography (IUCr)

    DOI： 10.1107/s1600577525008008 　

    eISSN：1600-5775

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    Printer toner is a fine powder material essential for fixing digital information on paper during the electrostatic digital printing process. To achieve fine printing, toner particles are composed of colored pigments, dyes, polymers, charge control agents and other surfactants. Toner particles have approximate dimensions of 5–10 µm. Therefore, the distribution of the constituents cannot be observed by transmission electron microscopy. In this study, we visualized the three-dimensional structure of a 5 µm-sized printer toner particle using cryogenic X-ray diffraction imaging tomography. A particle adsorbed on a silicon nitride membrane was rotated in the angular range from −78° to +78° against the direction of the incident X-rays at an angular step of 0.5°. From the 313 diffraction patterns collected, we reconstructed the three-dimensional electron density distribution of the toner particle at a resolution of 141 nm. The particle was wedge-shaped. The electron density distribution inside the particle was non-uniform. The high-electron-density regions were distributed near the surface. These formed a V-shaped structure at the tip of the wedge. With the help of powder diffraction, the high-electron-density regions were interpreted as microcrystals of silicon dioxide. Silicon dioxide crystals function as cleavage sites for toner particles prepared by the milling method. Based on the present results, we discuss the implications of the structure and methods to visualize it at a higher resolution.

  2. Experimental and numerical evaluation of the interfacial adhesion properties of additively manufactured carbon-fiber-reinforced thermoplastic/64 titanium hybrids 査読有り

    Keiichi Shirasu, Takeru Mizuno, Yamato Hoshikawa, Kosuke Ogawa, Yuki Takayama, Hironori Tohmyoh

    Composites Science and Technology　271　111338-111338　2025年10月

    出版者・発行元： Elsevier BV

    DOI： 10.1016/j.compscitech.2025.111338 　

    ISSN：0266-3538

  3. Comparative study of thermosetting CF/epoxy and thermoplastic CF/PAEK composite laminates: Effects of stacking sequences on open-hole strength 査読有り

    Kota Oine, Kazuki Ryuzono, Yoshiaki Kawagoe, Yamato Hoshikawa, Keiichi Shirasu, Yuki Takayama, Tomonaga Okabe

    Journal of Thermoplastic Composite Materials　2025年6月5日

    出版者・発行元： SAGE Publications

    DOI： 10.1177/08927057251344271 　

    ISSN：0892-7057

    eISSN：1530-7980

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    This study experimentally and numerically investigated the effect of stacking sequences on the open-hole compressive (OHC) and tensile (OHT) strength and failure mechanisms in carbon-fiber-reinforced thermoplastic (CFRTP) laminates. Specimens with stacking sequences containing a higher proportion of 0° layers and those with more ±45° layers were considered, denoted as “hard” and “soft” specimens, respectively. Strength prediction simulations for CFRTP were conducted using the three-dimensional extended finite element method, previously validated for carbon-fiber-reinforced thermosets (CFRTS). In the OHC test, kink-bands formed before matrix-dominated damage occurred for both hard and soft CFRTP specimens, while the opposite was found for CFRTS specimens due to the higher matrix toughness of CFRTP. Consequently, in soft CFRTP specimens, stress redistribution to the ±45° layers after kink-band formation led to gradual damage progression, achieving a strength comparable to CFRTS despite the higher 0°-layer compressive strength in CFRTS. The OHC simulation effectively predicted CFRTP failure using the maximum stress criterion. In the OHT test, CFRTP exhibited a higher strength than CFRTS in both hard and soft specimens due to the higher 0°-layer tensile strength in CFRTP. The OHT simulation accurately predicted abrupt failure in hard CFRTP specimens using the Weibull failure criterion but underestimated the strength of soft CFRTP specimens. This discrepancy arose because CFRTP exhibited less matrix-dominated damage, allowing stress redistribution to the ±45° layers after fiber breakage, leading to gradual damage progression. X-ray micro-computed-tomography observations confirmed that dispersed fiber breakages progressively connected through splitting cracks and propagated perpendicular to the fibers.

  4. Effect of preharvest boron spraying on the firmness and microstructure of winter‐harvested frozen broccoli among maturity types 査読有り

    Chotika Viriyarattanasak, Yuji Takemura, Tomoko Hashimoto, Kanako Hase, Yasumasa Ando, Namiko Nishida, Megumu Takahashi, Manato Ohishi, Masahiko Harata, Yuki Takayama, Masafumi Hidaka

    Journal of the Science of Food and Agriculture　105　(10)　5258-5267　2025年3月25日

    出版者・発行元： Wiley

    DOI： 10.1002/jsfa.14249 　

    ISSN：0022-5142

    eISSN：1097-0010

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    Abstract Background Softening of winter‐harvested frozen broccoli is a challenge. Application of boron (B) can improve fruit texture, but its effects on processed vegetables remain unexplored. This study is the first to investigate the impact of preharvest B spraying [0 (unsprayed), 195, 390, and 585 mg B L−1] on the firmness and microstructure of frozen broccoli, utilizing different cultivars harvested during the winter season. Result In the unsprayed group, ‘Pixel’ florets exhibited the highest firmness, followed by ‘Ohayo’, ‘Konnichiwa’, and ‘Kombanwa’, depending on the extent of low‐temperature exposure before harvest. Application of B did not enhance the firmness of ‘Pixel’ or ‘Ohayo’ florets after freeze–thawing, despite reducing water‐soluble pectin levels. Conversely, spraying with 585 mg B L−1 improved the firmness of freeze–thawed ‘Konnichiwa’ and ‘Kombanwa’ florets exposed to temperatures < 0 °C before harvesting by improving cell wall integrity via increased sodium carbonate (Na2CO3)‐soluble or chelator‐soluble pectin. Synchrotron X‐ray computed tomography analyses suggested the frozen state has a more ordered cell structure, whereas scanning electron microscopy revealed less cellular structure damage in the thawed state for ‘Konnichiwa’ florets sprayed with 585 mg B L−1 compared with unsprayed florets. Nevertheless, freeze–thawed ‘Konnichiwa’ and ‘Kombanwa’ florets sprayed with 585 mg B L−1 were considerably less firm than unsprayed ‘Pixel’ or ‘Ohayo’ florets. Conclusion Preharvest B spraying enhances the firmness of freeze–thawed broccoli grown at low temperatures by increasing cell wall rigidity and minimizing cell structure damage. Nonetheless, B application cannot fully overcome the consequences of exposure to low temperatures before harvest. © 2025 Society of Chemical Industry.

  5. 放射光コヒーレントX線による先端ナノイメージング技術 －腐食防食研究応用への期待－ 招待有り 査読有り

    高山裕貴

    表面技術　75　(10)　424-429　2024年10月

  6. Ptychographic phase retrieval via a deep-learning-assisted iterative algorithm 査読有り

    Koki Yamada, Natsuki Akaishi, Kohei Yatabe, Yuki Takayama

    Journal of Applied Crystallography　57　(5)　1323-1335　2024年8月19日

    出版者・発行元： International Union of Crystallography (IUCr)

    DOI： 10.1107/s1600576724006897 　

    eISSN：1600-5767

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    Ptychography is a powerful computational imaging technique with microscopic imaging capability and adaptability to various specimens. To obtain an imaging result, it requires a phase-retrieval algorithm whose performance directly determines the imaging quality. Recently, deep neural network (DNN)-based phase retrieval has been proposed to improve the imaging quality from the ordinary model-based iterative algorithms. However, the DNN-based methods have some limitations because of the sensitivity to changes in experimental conditions and the difficulty of collecting enough measured specimen images for training the DNN. To overcome these limitations, a ptychographic phase-retrieval algorithm that combines model-based and DNN-based approaches is proposed. This method exploits a DNN-based denoiser to assist an iterative algorithm like ePIE in finding better reconstruction images. This combination of DNN and iterative algorithms allows the measurement model to be explicitly incorporated into the DNN-based approach, improving its robustness to changes in experimental conditions. Furthermore, to circumvent the difficulty of collecting the training data, it is proposed that the DNN-based denoiser be trained without using actual measured specimen images but using a formula-driven supervised approach that systemically generates synthetic images. In experiments using simulation based on a hard X-ray ptychographic measurement system, the imaging capability of the proposed method was evaluated by comparing it with ePIE and rPIE. These results demonstrated that the proposed method was able to reconstruct higher-spatial-resolution images with half the number of iterations required by ePIE and rPIE, even for data with low illumination intensity. Also, the proposed method was shown to be robust to its hyperparameters. In addition, the proposed method was applied to ptychographic datasets of a Simens star chart and ink toner particles measured at SPring-8 BL24XU, which confirmed that it can successfully reconstruct images from measurement scans with a lower overlap ratio of the illumination regions than is required by ePIE and rPIE.

  7. Subgradient-projection-based stable phase-retrieval algorithm for X-ray ptychography

    Natsuki Akaishi, Koki Yamada, Kohei Yatabe, Yuki Takayama

    Journal of Applied Crystallography　57　(Pt 4)　1085-1097　2024年8月1日

    DOI： 10.1107/S1600576724004709 　

    ISSN：0021-8898

    eISSN：1600-5767

  8. マルチショット・コヒーレントX線回折イメージングの開発と実用化 招待有り 査読有り

    高山裕貴

    日本結晶学会誌　66　(2)　94-102　2024年5月31日

    出版者・発行元：

    DOI： 10.5940/jcrsj.66.94 　

    ISSN：0369-4585

    eISSN：1884-5576

  9. Towards sub-10 nm spatial resolution by tender X-ray ptychographic coherent diffraction imaging

    Nozomu Ishiguro, Fusae Kaneko, Masaki Abe, Yuki Takayama, Junya Yoshida, Taiki Hoshino, Shuntaro Takazawa, Hideshi Uematsu, Yuhei Sasaki, Naru Okawa, Keichi Takahashi, Hiroyuki Takizawa, Hiroyuki Kishimoto, Yukio Takahashi

    Applied Physics Express　17　(5)　2024年5月1日

    DOI： 10.35848/1882-0786/ad4846 　

    ISSN：1882-0778

    eISSN：1882-0786

  10. Redox-induced changes in nanostructures and electron densities of connected Pt–Fe catalysts for fuel cells revealed via in situ coherent X-ray diffraction 査読有り

    Yuki Takayama, Takanori Itoh, Hideto Imai, Hidenori Kuroki, Takeo Yamaguchi

    Japanese Journal of Applied Physics　63　(4)　048002　2024年3月21日

    出版者・発行元： IOP Publishing

    DOI： 10.35848/1347-4065/ad36a0 　

    ISSN：0021-4922

    eISSN：1347-4065

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    Abstract In a sustainable society, polymer electrolyte fuel cells are one of the promising energy devices for automobiles and residential uses because of their high energy efficiency. On the other hand, the current cathode catalysts are mainly made of precious metals such as Pt, and their activity and durability have become an issue for their widespread use. Carbon-free, connected Pt–Fe-alloy catalysts have been developed to solve this problem. However, their catalytic mechanism has not yet been clarified. In this study, redox-induced changes in the nanostructures and electron density distributions of the catalyst were investigated via coherent X-ray diffraction imaging.

  11. Noise tolerance of a sub-pixel shift method for upsampling diffraction patterns in coherent X-ray diffraction imaging 査読有り

    Yuki Takayama, Yasushi Kagoshima

    Japanese Journal of Applied Physics　63　(3)　038001　2024年3月1日

    出版者・発行元： IOP Publishing

    DOI： 10.35848/1347-4065/ad2b1a 　

    ISSN：0021-4922

    eISSN：1347-4065

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    Abstract In coherent X-ray diffraction imaging, speckles on a coherent diffraction pattern must be sampled at intervals sufficiently finer than the Nyquist interval, which imposes an upper limit on the sample size. To overcome the size limitation, a sub-pixel shift method for upsampling coherent diffraction patterns was proposed. This paper reports on the evaluation of the noise tolerance of the upsampling algorithm by a simulation. The quality of the images reconstructed from the upsampled diffraction pattern and pattern recorded by a detector with an equivalent pixel size was comparable when the optimum number of upsampling iterations is adopted.

  12. Protocol using similarity score and improved shrink-wrap algorithm for better convergence of phase-retrieval calculation in X-ray diffraction imaging 査読有り

    Syouyo Yoshida, Kosei Harada, So Uezu, Yuki Takayama, Masayoshi Nakasako

    Journal of Synchrotron Radiation　31　(1)　113-128　2024年1月1日

    出版者・発行元： International Union of Crystallography (IUCr)

    DOI： 10.1107/s1600577523009864 　

    eISSN：1600-5775

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    In X-ray diffraction imaging (XDI), electron density maps of a targeted particle are reconstructed computationally from the diffraction pattern alone using phase-retrieval (PR) algorithms. However, the PR calculations sometimes fail to yield realistic electron density maps that approximate the structure of the particle. This occurs due to the absence of structure amplitudes at and near the zero-scattering angle and the presence of Poisson noise in weak diffraction patterns. Consequently, the PR calculation becomes a bottleneck for XDI structure analyses. Here, a protocol to efficiently yield realistic maps is proposed. The protocol is based on the empirical observation that realistic maps tend to yield low similarity scores, as suggested in our prior study [Sekiguchi et al. (2017), J. Synchrotron Rad.24, 1024–1038]. Among independently and concurrently executed PR calculations, the protocol modifies all maps using the electron-density maps exhibiting low similarity scores. This approach, along with a new protocol for estimating particle shape, improved the probability of obtaining realistic maps for diffraction patterns from various aggregates of colloidal gold particles, as compared with PR calculations performed without the protocol. Consequently, the protocol has the potential to reduce computational costs in PR calculations and enable efficient XDI structure analysis of non-crystalline particles using synchrotron X-rays and X-ray free-electron laser pulses.

  13. Similarity score for screening phase-retrieved maps in X-ray diffraction imaging – characterization in reciprocal space 査読有り

    Yuki Takayama, Masayoshi Nakasako

    Journal of Synchrotron Radiation　31　(1)　95-112　2024年1月1日

    出版者・発行元： International Union of Crystallography (IUCr)

    DOI： 10.1107/s1600577523009827 　

    eISSN：1600-5775

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    X-ray diffraction imaging (XDI) is utilized for visualizing the structures of non-crystalline particles in material sciences and biology. In the structural analysis, phase-retrieval (PR) algorithms are applied to the diffraction amplitude data alone to reconstruct the electron density map of a specimen particle projected along the direction of the incident X-rays. However, PR calculations may not lead to good convergence because of a lack of diffraction patterns in small-angle regions and Poisson noise in X-ray detection. Therefore, the PR calculation is still a bottleneck for the efficient application of XDI in the structural analyses of non-crystalline particles. For screening maps from hundreds of trial PR calculations, we have been using a score and measuring the similarity between a pair of retrieved maps. Empirically, probable maps approximating the particle structures gave a score smaller than a threshold value, but the reasons for the effectiveness of the score are still unclear. In this study, the score is characterized in terms of the phase differences between the structure factors of the retrieved maps, the usefulness of the score in screening the maps retrieved from experimental diffraction patterns is demonstrated, and the effective resolution of similarity-score-selected maps is discussed.

  14. Ultrastructure and fractal property of chromosomes in close-to-native yeast nuclei visualized using X-ray laser diffraction 査読有り

    So Uezu, Takahiro Yamamoto, Mao Oide, Yuki Takayama, Koji Okajima, Amane Kobayashi, Masaki Yamamoto, Masayoshi Nakasako

    Scientific Reports　13　(1)　2023年7月5日

    出版者・発行元： Springer Science and Business Media LLC

    DOI： 10.1038/s41598-023-37733-6 　

    eISSN：2045-2322

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    Abstract Genome compaction and activity in the nucleus depend on spatiotemporal changes in the organization of chromatins in chromosomes. However, the direct imaging of the chromosome structures in the nuclei has been difficult and challenging. Herein, we directly visualized the structure of chromosomes in frozen-hydrated nuclei of budding yeast in the interphase using X-ray laser diffraction. The reconstructed projection electron density maps revealed inhomogeneous distributions of chromosomes, such as a 300 nm assembly and fibrous substructures in the elliptic-circular shaped nuclei of approximately 800 nm. In addition, from the diffraction patterns, we confirmed the absence of regular arrangements of chromosomes and chromatins with 400–20 nm spacing, and demonstrated that chromosomes were composed of self-similarly assembled substructural domains with an average radius of gyration of 58 nm and smooth surfaces. Based on these analyses, we constructed putative models to discuss the organization of 16 chromosomes, carrying DNA of 4.1 mm in 800 nm ellipsoid of the nucleus at the interphase. We anticipate the structural parameters on the fractal property of chromosomes and the experimental images to be a starting point for constructing more sophisticated 3D structural models of the nucleus.

  15. Ｘ線タイコグラフィによるゴム中ナノフィラー構造観察 査読有り

    砂川 正典, 山本 渥史, 岡本 泰志, 高山 裕貴

    SPring-8/SACLA利用研究成果集　11　(2)　153-155　2023年4月

  16. 兵庫県産黒大豆のミネラル及びポリフェノール類に着目した成分分析と機能性 査読有り

    吉村美紀, 加藤陽二, 和田裕子, 島田良子, 小林美幸, 赤松成基, 鯛かおる, 高山裕貴

    兵庫県立大学環境人間学部研究報告　25　41-46　2023年3月

  17. Practical design of the inverse-phase composite zone plate for improved depth of focus

    Yasushi Kagoshima, Yuki Takayama

    PROCEEDINGS OF THE 15TH INTERNATIONAL CONFERENCE ON X-RAY MICROSCOPY – XRM2022　2023年

    出版者・発行元： AIP Publishing

    DOI： 10.1063/5.0168281 　

    ISSN：0094-243X

  18. Phase retrieval based on a total-variation-regularized Poisson model for X-ray ptychographic imaging of low-contrast objects 査読有り

    Kohei Yatabe, Yuki Takayama

    Journal of Applied Crystallography　55　(4)　978-992　2022年8月

    出版者・発行元： International Union of Crystallography (IUCr)

    DOI： 10.1107/s1600576722005234 　

    ISSN：0021-8898

    eISSN：1600-5767

  19. SPring-8におけるコヒーレントX線回折イメージングの展開 招待有り 査読有り

    高山裕貴, 中迫雅由

    日本結晶学会誌　64　(1)　41-49　2022年2月28日

    出版者・発行元：

    DOI： 10.5940/jcrsj.64.41 　

    ISSN：0369-4585

    eISSN：1884-5576

  20. Dynamic nanoimaging of extended objects via hard X-ray multiple-shot coherent diffraction with projection illumination optics 査読有り

    Yuki Takayama, Keizo Fukuda, Motoki Kawashima, Yuki Aoi, Daiki Shigematsu, Tatsuki Akada, Takumi Ikeda, Yasushi Kagoshima

    Communications Physics　4　(1)　2021年12月

    出版者・発行元： Springer Science and Business Media {LLC}

    DOI： 10.1038/s42005-021-00539-x 　

    eISSN：2399-3650

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    <title>Abstract</title>The quest for understanding the structural mechanisms of material properties and biological cell functions has led to the active development of coherent diffraction imaging (CDI) and its variants in the hard X-ray regime. Herein, we propose multiple-shot CDI, a full-field CDI technique dedicated to the visualisation of local nanostructural dynamics in extended objects at a spatio-temporal resolution beyond that of current instrumentation limitations. Multiple-shot CDI reconstructs a “movie” of local dynamics from time-evolving diffraction patterns, which is compatible with a robust scanning variant, ptychography. We developed projection illumination optics to produce a probe with a well-defined illumination area and a phase retrieval algorithm, establishing a spatio-temporal smoothness constraint for the reliable reconstruction of dynamic images. The numerical simulations and proof-of-concept experiment using synchrotron hard X-rays demonstrated the capability of visualising a dynamic nanostructured object at a frame rate of 10 Hz or higher.

  21. Optical transfer function of inverse-phase composite zone plate devised for deep-focusing X-ray microscopes 査読有り

    Yasushi Kagoshima, Yuki Takayama

    Japanese Journal of Applied Physics　60　(11)　118001-118001　2021年11月1日

    出版者・発行元： IOP Publishing

    DOI： 10.35848/1347-4065/ac2e69 　

    ISSN：0021-4922

    eISSN：1347-4065

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    Abstract The optical transfer function (OTF) of an inverse-phase composite zone plate under incoherent illumination was numerically evaluated by convoluting the point spread function (PSF) and transmission distribution of a model sample with gradually changing spatial frequency. The PSF used in the simulation was obtained from our previous study on deep-focusing X-ray microscopes. As expected, the contrast in the sample image was lesser than that produced by a conventional zone plate. Phase-reversed contrast—spurious resolution—appeared at a high spatial frequency. Although the calculated OTF is slightly aberrated, its deep-focusing capability is advantageous for X-ray microimaging of thick samples.

  22. 放射光X線タイコグラフィによる自動車塗膜の三次元観察 査読有り

    末広省吾, 幸坂崇, 小林秀雄, 高山裕貴

    塗装工学　56　(6)　204-210　2021年6月30日

  23. Common architectures in cyanobacteria Prochlorococcus cells visualized by X-ray diffraction imaging using X-ray free electron laser. 国際誌 査読有り

    Amane Kobayashi, Yuki Takayama, Takeshi Hirakawa, Koji Okajima, Mao Oide, Tomotaka Oroguchi, Yayoi Inui, Masaki Yamamoto, Sachihiro Matsunaga, Masayoshi Nakasako

    Scientific reports　11　(1)　3877-3877　2021年2月16日

    DOI： 10.1038/s41598-021-83401-y 　

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    Visualization of intracellular structures and their spatial organization inside cells without any modification is essential to understand the mechanisms underlying the biological functions of cells. Here, we investigated the intracellular structure of cyanobacteria Prochlorococcus in the interphase by X-ray diffraction imaging using X-ray free-electron laser. A number of diffraction patterns from single cells smaller than 1 µm in size were collected with high signal-to-noise ratio with a resolution of up to 30 nm. From diffraction patterns, a set of electron density maps projected along the direction of the incident X-ray were retrieved with high reliability. The most characteristic structure found to be common among the cells was a C-shaped arrangement of 100-nm sized high-density spots, which surrounded a low-density area of 100 nm. Furthermore, a three-dimensional map reconstructed from the projection maps of individual cells was non-uniform, indicating the presence of common structures among cyanobacteria cells in the interphase. By referring to the fluorescent images for distributions of thylakoid membranes, nucleoids, and carboxysomes, we inferred and represented their spatial arrangements in the three-dimensional map. The arrangement allowed us to discuss the relevance of the intracellular organization to the biological functions of cyanobacteria.

  24. Lanthanum silicate-based layered electrolyte for intermediate-temperature fuel cell application 査読有り

    Atsushi Mineshige, Atsushi Saito, Mio Kobayashi, Hikaru Hayakawa, Mizuki Momai, Tetsuo Yazawa, Hideki Yoshioka, Mitsumasa Sakao, Ryohei Mori, Yuki Takayama, Yasushi Kagoshima, Junji Matsui

    Journal of Power Sources　475　2020年11月1日

    DOI： 10.1016/j.jpowsour.2020.228543 　

    ISSN：0378-7753

  25. Domain Organization in Plant Blue-Light Receptor Phototropin2 of Arabidopsis thaliana Studied by Small-Angle X-ray Scattering. 国際誌 査読有り

    Masayoshi Nakasako, Mao Oide, Yuki Takayama, Tomotaka Oroguchi, Koji Okajima

    International journal of molecular sciences　21　(18)　2020年9月10日

    DOI： 10.3390/ijms21186638 　

    詳細を見る 詳細を閉じる

    Phototropin2 (phot2) is a blue-light (BL) receptor protein that regulates the BL-dependent activities of plants for efficient photosynthesis. Phot2 is composed of two light-oxygen-voltage sensing domains (LOV1 and LOV2) to absorb BL, and a kinase domain. Photo-activated LOV domains, especially LOV2, play a major role in photo-dependent increase in the phosphorylation activity of the kinase domain. The atomic details of the overall structure of phot2 and the intramolecular mechanism to convert BL energy to a phosphorylation signal remain unknown. We performed structural studies on the LOV fragments LOV1, LOV2, LOV2-linker, and LOV2-kinase, and full-length phot2, using small-angle X-ray scattering (SAXS). The aim of the study was to understand structural changes under BL irradiation and discuss the molecular mechanism that enhance the phosphorylation activity under BL. SAXS is a suitable technique for visualizing molecular structures of proteins in solution at low resolution and is advantageous for monitoring their structural changes in the presence of external physical and/or chemical stimuli. Structural parameters and molecular models of the recombinant specimens were obtained from SAXS profiles in the dark, under BL irradiation, and after dark reversion. LOV1, LOV2, and LOV2-linker fragments displayed minimal structural changes. However, BL-induced rearrangements of functional domains were noted for LOV2-kinase and full-length phot2. Based on the molecular model together with the absorption measurements and biochemical assays, we discuss the intramolecular interactions and domain motions necessary for BL-enhanced phosphorylation activity of phot2.

  26. Measurement of the horizontal beam emittance of undulator radiation by tandem-double-slit optical system. 国際誌 査読有り

    Yasushi Kagoshima, Tatsuki Akada, Takumi Ikeda, Motoki Kawashima, Yuki Aoi, Yuki Takayama

    Journal of synchrotron radiation　27　(Pt 3)　799-803　2020年5月1日

    DOI： 10.1107/S1600577520004415 　

    詳細を見る 詳細を閉じる

    A tandem-double-slit optical system was constructed to evaluate the practical beam emittance of undulator radiation. The optical system was a combination of an upstream slit (S1) and downstream slit (S2) aligned on the optical axis with an appropriate separation. The intensity distribution after the double slits, I(x1, x2), was measured by scanning S1 and S2 in the horizontal direction. Coordinates having 1/\sqrt e intensity were extracted from I(x1, x2), whose contour provided the standard deviation ellipse in the x1-x2 space. I(x1, x2) was converted to the corresponding distribution in the phase space, I(x1, x1'). The horizontal beam emittance was evaluated to be 3.1 nm rad, which was larger than the value of 2.4 nm rad estimated by using ray-tracing. It was found that the increase was mainly due to an increase in beam divergence rather than size.

  27. Methods and application of coherent X-ray diffraction imaging of noncrystalline particles. 国際誌 査読有り

    Masayoshi Nakasako, Amane Kobayashi, Yuki Takayama, Kenta Asakura, Mao Oide, Koji Okajima, Tomotaka Oroguchi, Masaki Yamamoto

    Biophysical reviews　12　(2)　541-567　2020年4月

    DOI： 10.1007/s12551-020-00690-9 　

    詳細を見る 詳細を閉じる

    Microscopic imaging techniques have been developed to visualize events occurring in biological cells. Coherent X-ray diffraction imaging is one of the techniques applicable to structural analyses of cells and organelles, which have never been crystallized. In the experiment, a single noncrystalline particle is illuminated by an X-ray beam with almost complete spatial coherence. The structure of the particle projected along the direction of the beam is, in principle, retrieved from a finely recorded diffraction pattern alone by using iterative phase-retrieval algorithms. Here, we describe fundamental theory and experimental methods of coherent X-ray diffraction imaging and the recent application in structural studies of noncrystalline specimens by using X-rays available at Super Photon Ring of 8-Gev and SPring-8 Angstrom Compact Free Electron Laser in Japan.

  28. 特別企画 試料環境シリーズ(7) 第4回「温度」 4-1 蛋白質結晶の低温X線回折実験 ～低温 1～ 招待有り 査読有り

    中迫雅由, 高山裕貴, 山本雅貴

    放射光　33　(2)　101-111　2020年1月

  29. 貯蔵期間の異なる手延そうめんの性状と構造観察 査読有り

    細田捺希, 高山裕貴, 赤田樹, 青井雄幹, 原信岳, 吉村美紀

    日本調理科学会誌　52　(6)　386-394　2019年12月

  30. Inverse-phase composite zone plate providing deeper focus than the normal diffraction-limited depth of X-ray microbeams. 国際誌 査読有り

    Yasushi Kagoshima, Yuki Takayama

    Journal of synchrotron radiation　26　(Pt 1)　52-58　2019年1月1日

    DOI： 10.1107/S1600577518016703 　

    詳細を見る 詳細を閉じる

    A novel type of zone plate (ZP), termed an inverse-phase composite ZP, is proposed to gain a deeper focus than the standard diffraction-limited depth of focus, with little reduction in spatial resolution. The structure is a combination of an inner ZP functioning as a conventional phase ZP and an outer ZP functioning with third-order diffraction with opposite phase to the inner ZP. Two-dimensional complex amplitude distributions neighboring the focal point were calculated using a wave-optical approach of diffraction integration with a monochromatic plane-wave illumination, where one dimension is the radial direction and the other dimension is the optical-axis direction. The depth of focus and the spatial resolution were examined as the main focusing properties. Two characteristic promising cases regarding the depth of focus were found: a pit-intensity focus with the deepest depth of focus, and a flat-intensity focus with deeper depth of focus than usual ZPs. It was found that twice the depth of focus could be expected with little reduction in the spatial resolution for 10 keV X-ray energy, tantalum zone material, 84 nm minimum fabrication zone width, and zone thickness of 2.645 µm. It was also found that the depth of focus and the spatial resolution were almost unchanged in the photon energy range from 8 to 12 keV. The inverse-phase composite ZP has high potential for use in analysis of practical thick samples in X-ray microbeam applications.

  31. Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation 査読有り

    Kobayashi A, Takayama Y, Okajima K, Oide M, Yamamoto T, Sekiguchi Y, Oroguchi T, Nakasako M, Kohmura Y, Yamamoto M, Hoshi T, Torizuka Y

    Journal of Synchrotron Radiation　25　(6)　1803-1818　2018年11月

    DOI： 10.1107/S1600577518012687 　

  32. Proposal for Inverse-Phase Composite Zone Plate for Deeper Depth of Focus 査読有り

    Kagoshima Y, Takayama Y

    Microscopy and Microanalysis　24　(S2)　278-279　2018年8月

    DOI： 10.1017/S1431927618013739 　

  33. Cryogenic Coherent X-ray Diffraction Imaging Techniques for Structural Analyses of Biological Cells and Cellular Organelles 査読有り

    Kobayashi A, Takayama Y, Oroguchi T, Okajima K, Oide M, Yamamoto M, Nakasako M

    Microscopy and Microanalysis　24　(S2)　14-15　2018年8月

    DOI： 10.1017/S1431927618012503 　

  34. Atmospheric coherent X-ray diffraction imaging for in situ structural analysis at SPring-8 Hyogo beamline BL24XU. 国際誌 査読有り

    Yuki Takayama, Yuki Takami, Keizo Fukuda, Takamasa Miyagawa, Yasushi Kagoshima

    Journal of synchrotron radiation　25　(Pt 4)　1229-1237　2018年7月1日

    DOI： 10.1107/S1600577518006410 　

    ISSN：0909-0495

    詳細を見る 詳細を閉じる

    Coherent X-ray diffraction imaging (CXDI) is a promising technique for non-destructive structural analysis of micrometre-sized non-crystalline samples at nanometre resolutions. This article describes an atmospheric CXDI system developed at SPring-8 Hyogo beamline BL24XU for in situ structural analysis and designed for experiments at a photon energy of 8 keV. This relatively high X-ray energy enables experiments to be conducted under ambient atmospheric conditions, which is advantageous for the visualization of samples in native states. The illumination condition with pinhole-slit optics is optimized according to wave propagation calculations based on the Fresnel-Kirchhoff diffraction formula so that the sample is irradiated by X-rays with a plane wavefront and high photon flux of ∼1 × 1010 photons/16 µmø(FWHM)/s. This work demonstrates the imaging performance of the atmospheric CXDI system by visualizing internal voids of sub-micrometre-sized colloidal gold particles at a resolution of 29.1 nm. A CXDI experiment with a single macroporous silica particle under controlled humidity was also performed by installing a home-made humidity control device in the system. The in situ observation of changes in diffraction patterns according to humidity variation and reconstruction of projected electron-density maps at 5.2% RH (relative humidity) and 82.6% RH at resolutions of 133 and 217 nm, respectively, were accomplished.

  35. Preparation of lanthanum silicate electrolyte with high conductivity and high chemical stability 査読有り

    Atsushi Mineshige, Hikaru Hayakawa, Takuma Nishimoto, Akie Heguri, Tetsuo Yazawa, Yuki Takayama, Yasushi Kagoshima, Hidekazu Takano, Shingo Takeda, Junji Matsui

    Solid State Ionics　319　223-227　2018年6月

    DOI： 10.1016/j.ssi.2018.02.002 　

    ISSN：0167-2738

  36. 生物細胞の低温X線回折イメージング 招待有り 査読有り

    中迫 雅由, 高山 裕貴, 小林 周, 山本 隆寛, 大出 真央, 岡島 公司, 苙口 友隆, 山本 雅貴

    放射光 : 日本放射光学会誌 = Journal of the Japanese Society for Synchrotron Radiation Research　31　(3)　189-201　2018年5月

    出版者・発行元： 日本放射光学会

    ISSN：0914-9287

  37. Common structural features of toxic intermediates from α-synuclein and GroES fibrillogenesis detected using cryogenic coherent X-ray diffraction imaging. 査読有り

    Kameda H, Usugi S, Kobayashi M, Fukui N, Lee S, Hongo K, Mizobata T, Sekiguchi Y, Masaki Y, Kobayashi A, Oroguchi T, Nakasako M, Takayama Y, Yamamoto M, Kawata Y

    Journal of biochemistry　161　(1)　55-65　2017年1月

    DOI： 10.1093/jb/mvw052 　

    ISSN：0021-924X

    eISSN：1756-2651

  38. Blue Light-excited Light-Oxygen-Voltage-sensing Domain 2 (LOV2) Triggers a Rearrangement of the Kinase Domain to Induce Phosphorylation Activity in Arabidopsis Phototropin1 査読有り

    Mao Oide, Koji Okajima, Sachiko Kashojiya, Yuki Takayama, Tomotaka Oroguchi, Takaaki Hikima, Masaki Yamamoto, Masayoshi Nakasako

    JOURNAL OF BIOLOGICAL CHEMISTRY　291　(38)　19975-19984　2016年9月

    DOI： 10.1074/jbc.M116.735787 　

    ISSN：0021-9258

    eISSN：1083-351X

  39. TAKASAGO-6 apparatus for cryogenic coherent X-ray diffraction imaging of biological non-crystalline particles using X-ray free electron laser at SACLA 査読有り

    Amane Kobayashi, Yuki Sekiguchi, Yuki Takayama, Tomotaka Oroguchi, Keiya Shirahama, Yasufumi Torizuka, Masahiro Manoda, Masayoshi Nakasako, Masaki Yamamoto

    REVIEW OF SCIENTIFIC INSTRUMENTS　87　(5)　053109　2016年6月

    DOI： 10.1063/1.4948317 　

    ISSN：0034-6748

    eISSN：1089-7623

  40. Cryogenic coherent X-ray diffraction imaging of biological samples at SACLA: a correlative approach with cryo-electron and light microscopy 招待有り 査読有り

    Yuki Takayama, Koji Yonekura

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES　72　(Pt 2)　179-189　2016年3月

    DOI： 10.1107/S2053273315023980 　

    ISSN：2053-2733

  41. 金コロイド粒子を利用した非結晶生体試料の高分解能・高精度コヒーレントX線回折イメージング 招待有り 査読有り

    高山裕貴, 眞木さおり, 苙口友隆, 中迫雅由, 米倉功治

    Isotope News　739　(11)　9-14　2015年11月

  42. Cryogenic coherent x-ray diffraction imaging for biological non-crystalline particles using the KOTOBUKI-1 diffraction apparatus at SACLA 査読有り

    Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Yu Masaki, Asahi Fukuda, Saki Hashimoto, Masayoshi Nakasako, Yuichi Ichikawa, Hitoshi Kurumizaka, Mitsuhiro Shimizu, Yayoi Inui, Sachihiro Matsunaga, Takayuki Kato, Keiichi Namba, Keiichi Yamaguchi, Kazuo Kuwata, Hiroshi Kameda, Naoya Fukui, Yasushi Kawata, Takashi Kameshima, Yuki Takayama, Koji Yonekura, Masaki Yamamoto

    JOURNAL OF PHYSICS B-ATOMIC MOLECULAR AND OPTICAL PHYSICS　48　(18)　184003　2015年9月

    DOI： 10.1088/0953-4075/48/18/184003 　

    ISSN：0953-4075

    eISSN：1361-6455

  43. Coherent X-Ray Diffraction Imaging of Chloroplasts from Cyanidioschyzon merolae by Using X-Ray Free Electron Laser 査読有り

    Yuki Takayama, Yayoi Inui, Yuki Sekiguchi, Amane Kobayashi, Tomotaka Oroguchi, Masaki Yamamoto, Sachihiro Matsunaga, Masayoshi Nakasako

    PLANT AND CELL PHYSIOLOGY　56　(7)　1272-1286　2015年7月

    DOI： 10.1093/pcp/pcv032 　

    ISSN：0032-0781

    eISSN：1471-9053

  44. Signal enhancement and Patterson-search phasing for high-spatial-resolution coherent X-ray diffraction imaging of biological objects 査読有り

    Yuki Takayama, Saori Maki-Yonekura, Tomotaka Oroguchi, Masayoshi Nakasako, Koji Yonekura

    SCIENTIFIC REPORTS　5　8074　2015年1月

    DOI： 10.1038/srep08074 　

    ISSN：2045-2322

  45. IDATEN and G-SITENNO: GUI-assisted software for coherent X-ray diffraction imaging experiments and data analyses at SACLA 査読有り

    Yuki Sekiguchi, Masaki Yamamoto, Tomotaka Oroguchi, Yuki Takayama, Shigeyuki Suzuki, Masayoshi Nakasako

    Journal of Synchrotron Radiation　21　(6)　1378-1383　2014年11月1日

    出版者・発行元： International Union of Crystallography

    DOI： 10.1107/S1600577514017111 　

    ISSN：1600-5775 0909-0495

  46. Dark-field phase retrieval under the constraint of the Friedel symmetry in coherent X-ray diffraction imaging 査読有り

    Amane Kobayashi, Yuki Sekiguchi, Yuki Takayama, Tomotaka Oroguchi, Masayoshi Nakasako

    OPTICS EXPRESS　22　(23)　27892-27909　2014年11月

    DOI： 10.1364/OE.22.027892 　

    ISSN：1094-4087

  47. Single-shot three-dimensional structure determination of nanocrystals with femtosecond X-ray free-electron laser pulses 査読有り

    Rui Xu, Huaidong Jiang, Changyong Song, Jose A. Rodriguez, Zhifeng Huang, Chien-Chun Chen, Daewoong Nam, Jaehyun Park, Marcus Gallagher-Jones, Sangsoo Kim, Sunam Kim, Akihiro Suzuki, Yuki Takayama, Tomotaka Oroguchi, Yukio Takahashi, Jiadong Fan, Yunfei Zou, Takaki Hatsui, Yuichi Inubushi, Takashi Kameshima, Koji Yonekura, Kensuke Tono, Tadashi Togashi, Takahiro Sato, Masaki Yamamoto, Masayoshi Nakasako, Makina Yabashi, Tetsuya Ishikawa, Jianwei Miao

    NATURE COMMUNICATIONS　5　4061　2014年6月

    DOI： 10.1038/ncomms5061 　

    ISSN：2041-1723

  48. Data processing software suite SITENNO for coherent X-ray diffraction imaging using the X-ray free-electron laser SACLA 査読有り

    Yuki Sekiguchi, Tomotaka Oroguchi, Yuki Takayama, Masayoshi Nakasako

    JOURNAL OF SYNCHROTRON RADIATION　21　(Pt 3)　600-612　2014年5月

    DOI： 10.1107/S1600577514003439 　

    ISSN：0909-0495

    eISSN：1600-5775

  49. X線自由電子レーザーを用いた非結晶粒子のコヒーレントX線回折イメージング 査読有り

    中迫 雅由, 眞木 さおり, 引間 孝明, 高橋 幸生, 鈴木 明大, 松永 幸大, 乾 弥生, 登野 健介, 亀島 敬, 城地 保昌, 犬伏 雄一, 苙口 友隆, 星 貴彦, 関口 優希, 小林 周, 橋本 早紀, 白濱 圭也, 山本 雅貴, 高山 裕貴, 米倉 功治

    日本結晶学会誌　56　(1)　27-35　2014年

    出版者・発行元： 日本結晶学会

    DOI： 10.5940/jcrsj.56.27 　

    ISSN：0369-4585

    詳細を見る 詳細を閉じる

    Coherent X-ray diffraction imaging（CXDI）is a lens-less imaging technique that can visualize the structures of non-crystalline particles with micro- to sub-micrometer dimensions. In CXDI experiments, spatially isolated particles are irradiated by X-ray beam with high transverse coherence. Projection images of the particles along the incident X-ray direction are then directly reconstructed from the diffraction amplitude using a phase retrieval algorithm. Here we report CXDI experiments carried out at the X-ray free electron laser（XFEL）facility SACLA, and describe the theoretical background of CXDI, development of a diffractometer and data processing.

  50. Light-induced Conformational Changes of LOV1 (Light Oxygen Voltage-sensing Domain 1) and LOV2 Relative to the Kinase Domain and Regulation of Kinase Activity in Chlamydomonas Phototropin 査読有り

    Koji Okajima, Yusuke Aihara, Yuki Takayama, Mihoko Nakajima, Sachiko Kashojiya, Takaaki Hikima, Tomotaka Oroguchi, Amane Kobayashi, Yuki Sekiguchi, Masaki Yamamoto, Tomomi Suzuki, Akira Nagatani, Masayoshi Nakasako, Satoru Tokutomi

    JOURNAL OF BIOLOGICAL CHEMISTRY　289　(1)　413-422　2014年1月

    DOI： 10.1074/jbc.M113.515403 　

    ISSN：0021-9258

    eISSN：1083-351X

  51. Coherent Diffraction Imaging Analysis of Shape-Controlled Nanoparticles with Focused Hard X-ray Free-Electron Laser Pulses 査読有り

    Yukio Takahashi, Akihiro Suzuki, Nobuyuki Zettsu, Tomotaka Oroguchi, Yuki Takayama, Yuki Sekiguchi, Amane Kobayashi, Masaki Yamamoto, Masayoshi Nakasako

    NANO LETTERS　13　(12)　6028-6032　2013年12月

    DOI： 10.1021/nl403247x 　

    ISSN：1530-6984

    eISSN：1530-6992

  52. KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging 査読有り

    Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Keiya Shirahama, Masaki Yamamoto, Takaaki Hikima, Koji Yonekura, Saori Maki-Yonekura, Yoshiki Kohmura, Yuichi Inubushi, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Inui, Kensuke Tono, Takashi Kameshima, Yasumasa Joti, Takahiko Hoshi

    REVIEW OF SCIENTIFIC INSTRUMENTS　84　(9)　093705　2013年9月

    DOI： 10.1063/1.4822123 　

    ISSN：0034-6748

    eISSN：1089-7623

  53. 蛋白質水和構造の実験研究 査読有り

    中迫雅由, 苙口友隆, 高山裕貴, 中島真, 松井夕花

    アンサンブル　15　(1)　7-18　2013年1月

    出版者・発行元： 分子シミュレーション研究会

    DOI： 10.11436/mssj.15.7 　

    ISSN：1884-6750

  54. コヒーレントX線回折イメージング構造解析理論の開発と展望 査読有り

    中迫 雅由, 笠口 友隆, 高山 裕貴, 小林 周, 児玉 渉, 坂本 啓太, 吉留 崇, 池口 満徳

    放射光　26　(1)　11-25　2013年1月

    出版者・発行元： 日本放射光学会

    ISSN：0914-9287

  55. 非結晶粒子のコヒーレントX線回折イメージング 査読有り

    中迫 雅由, 高山 裕貴, 苙口 友隆, 白濱 圭也, 関口 優希, 山本 雅貴, 米倉 功治, 引間 孝明, 眞木 さおり, 高橋 幸生, 鈴木 明大, 松永 幸大, 加藤 翔一, 星 貴彦

    レーザー研究　40　(9)　680-686　2012年11月

    ISSN：0387-0200

  56. X線自由電子レーザーを用いた非結晶粒子のコヒーレントX線回折イメージング実験 査読有り

    中迫雅由, 高山裕貴, 苙口友隆, 白濱圭也, 関口優希, 山本雅貴, 米倉功治, 引間孝明, 眞木‐さおり, 高橋幸生, 鈴木明大, 松永幸大, 加藤翔一, 星貴彦

    オプトロニクス　368　(8)　101-106　2012年8月

    出版者・発行元： オプトロニクス社

    ISSN：0286-9659

  57. Humidity-controlled preparation of frozen-hydrated biological samples for cryogenic coherent x-ray diffraction microscopy 査読有り

    Yuki Takayama, Masayoshi Nakasako

    REVIEW OF SCIENTIFIC INSTRUMENTS　83　(5)　054301　2012年5月

    DOI： 10.1063/1.4718359 　

    ISSN：0034-6748

  58. A few low-frequency normal modes predominantly contribute to conformational responses of hen egg white lysozyme in the tetragonal crystal to variations of molecular packing controlled by environmental humidity 査読有り

    Yuki Takayama, Masayoshi Nakasako

    BIOPHYSICAL CHEMISTRY　159　(2-3)　237-246　2011年12月

    DOI： 10.1016/j.bpc.2011.07.001 　

    ISSN：0301-4622

    eISSN：1873-4200

  59. Light-Induced Movement of the LOV2 Domain in an Asp720Asn Mutant LOV2-Kinase Fragment of Arabidopsis Phototropin 2 査読有り

    Yuki Takayama, Masayoshi Nakasako, Koji Okajima, Aya Iwata, Sachiko Kashojiya, Yuka Matsui, Satoru Tokutomi

    BIOCHEMISTRY　50　(7)　1174-1183　2011年2月

    DOI： 10.1021/bi101689b 　

    ISSN：0006-2960

  60. Redox-Dependent Domain Rearrangement of Protein Disulfide Isomerase from a Thermophilic Fungus 査読有り

    Masayoshi Nakasako, Aya Maeno, Eiji Kurimoto, Takushi Harada, Yoshiki Yamaguchi, Toshihiko Oka, Yuki Takayama, Aya Iwata, Koichi Kato

    BIOCHEMISTRY　49　(32)　6953-6962　2010年8月

    DOI： 10.1021/bi1006089 　

    ISSN：0006-2960

︎全件表示 ︎最初の5件までを表示

MISC 8

  1. 次世代放射光施設「NanoTerasu（ナノテラス）」のバイオサイエンスでの活用 招待有り

    高山裕貴, 原田昌彦, 村松淳司, 日髙將文

    バイオサイエンスとインダストリー　83　(1)　2025年1月

  2. BL24XU (Hyogo ID)

    Y. Urushihara, Y. Takayama, S. Kuwamoto, L. Cong, M. Yoshimura, Y. Tsusaka, Y. Kagoshima, S. Suzuki, K. Yokoyama, J. Matsui

    SPring-8 / SACLA annual report　FY2021　145-146　2023年7月

  3. 食・農領域での次世代放射光利活用の推進 招待有り

    原田昌彦, 高山裕貴, 日髙將文

    MICROOPTICS NEWS　41　23-28　2023年

  4. Dynamic nanoimaging of extended objects via hard X-ray multiple-shot coherent diffraction with projection illumination optics 招待有り

    Yuki Takayama, Yasushi Kagoshima

    SPring-8/SACLA Research Frontiers 2021　56-57　2022年

  5. コヒーレントX線を用いたレンズレス時空間階層イメージング法の開発 招待有り

    高山裕貴, 篭島靖

    電気学会 光・量子デバイス研究会 研究会論文　OQD21068　2021年12月

  6. コヒーレントX線を用いたレンズレス時空間階層イメージング法の開発（SPRUC 2021 Young Scientist Award受賞 研究報告） 招待有り 査読有り

    高山裕貴

    SPring-8 / SACLA 利用者情報　26　349-355　2021年

  7. BL24XU (Hyogo ID)

    Yuki Takayama, Yoshiyuki Tsusaka, Yasushi Kagoshima, Shigeo Kuwamoto, Yoshimasa Urushihara, Lei Li, Souichi Nose, Taiki Sudo, Masashi Yoshimura, Kazushi Yokoyama, Junji Matsui

    SPring-8・SACLA Annual Report　FY2018　132-135　2020年7月

  8. Signal enhancement and Patterson-search phasing for higher-spatial-resolution coherent X-ray diffraction imaging of biological objects 招待有り 査読有り

    Takayama Y, Yonekura K

    SPring-8/SACLA Research Frontiers 2015　132-133　2016年

︎全件表示 ︎最初の5件までを表示

書籍等出版物 1

  1. 'Coherent X-ray diffraction imaging of Cyanidioschyzon merolae' in Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology

    Sekiguchi Y, Kobayashi A, Takayama Y, Oide M, Fukuda A, Yamamoto T, Okajima K, Oroguchi T, Hirakawa T, Inui Y, Matsunaga S, Yamamoto M, Nakasako M

    Springer　2018年2月

    ISBN: 9789811061004

講演・口頭発表等 43

  1. Towards the utilization of the new Japanese synchrotron NanoTerasu at Tohoku University for food and agricultural products 招待有り

    M. Harata, Y. Takayama, A. Muramatsu, M. Hidaka, Y. Oma

    The 4th International Pan American Light Sources for Agriculture (PALSA 2025)　2025年8月22日

  2. Synchrotron Radiation-Based Characterization of Food and Agricultural Materials by A-Sync in Tohoku University

    M. Hidaka, Y. Takayama, Y. Oma, A. Muramatsu, M. Harata

    The 4th International Pan American Light Sources for Agriculture (PALSA 2025)　2025年8月20日

  3. Visualization of Spatially Hierarchical Structures in Food and Cells via Coherent X-ray Diffraction Imaging

    Y. Takayama, M. Hidaka, Y. Oma, A. Muramatsu, M. Harata

    The 4th International Pan American Light Sources for Agriculture (PALSA 2025)　2025年8月20日

  4. Decoding Temporal Dynamics from Spatial Landscape: Examples from Iron Corrosion and Somen Noodles 招待有り

    Y. Takayama

    Time and Space in Non-Equilibrium Materials 〜Exploring the Relations of Time and Space〜　2025年8月13日

  5. Visualization of micro- to nano-structures in food and agricultural samples via coherent X-rays 招待有り

    Y. Takayama

    Tohoku Forum for Creativity: Synchrotron Light for Future Foods　2024年9月

  6. Manifold-Learning Approach for Extracting Molecular Information and Predicting of Physical Properties

    S. Arai, G. Kikugawa, Y. Takayama, T. Yoshidome

    The 17th Mini-Symposium on Liquids 2024　2024年7月

  7. X-ray diffraction imaging tomography at cryogenic temperature is powerful to reveal 3D structures of biological specimens

    K. Harada, Y. Takayama, M. Nakasako

    21st IUPAB Congress 2024　2024年6月

  8. Cryogenic X-ray Ptychographic Imaging of Cultured Cells toward Visualization of Hierarchical Structures of Chromatin

    Y. Kinami, K. Nishimagi, K. Harada, M. Nakasako, Y. Oma, M. Harata, Y. Takayama

    21st IUPAB Congress 2024　2024年6月

  9. Coherent X-ray Imaging of the Nanostructures of Covalent Organic Frameworks in the initial Synthesis Process

    T. Nishio, Y. Takayama, K. Fujiu, M. Kin, T. Ono

    UVSOR-III + MAX IV International Workshop: Frontier of Soft X-ray Spectroscopy for Chemical Processes in Solutions　2023年10月

  10. Protocol to steer phase-retrieval calculation in X-ray diffraction imaging

    S. Yoshida, K. Harada, S. Uezu, Y. Takayama, M. Nakasako

    60 years of Synchrotron Radiation in Japan (JPSR60)　2023年10月

  11. Visualization of micro/nano hierarchical structures via synchrotron X-rays

    Y. Takayama

    WUR-TU Future Prospects in Agriculture based on Immunity, Food Science, and Synchrotron Light　2023年1月

  12. Protocol for improving accuracy in phase-retrieval calculations in X-ray diffraction imaging

    S. Yoshida, S. Uezu, Y. Takayama, M. Nakasako

    Asia Oceania International Conference on Synchrotron Radiation Instruments 2022　2022年11月

  13. Spatiotemporal nanoimaging of extended objects via multiple-shot coherent diffraction imaging and ptychographic tomography 招待有り

    Y. Takayama, A. Matsuzaki, Y. Kagoshima

    Asia Oceania International Conference on Synchrotron Radiation Instruments 2022　2022年11月

  14. Observation of Shear-Induced Orientation Change of Grease Thickener Structure Using Ultra-Small-Angle X-ray Scattering

    T. Noda, Y. Takayama, S. Kuwamoto, K. Sonoda, H. Washizu

    7th World Tribology Congress WTC 2022, Lyon, France　2022年7月

  15. Spatiotemporal nanoimaging of extended objects via multiple-shot coherent diffraction imaging and ptychographic tomography 招待有り

    Y. Takayama, A. Matsuzaki, Y. Kagoshima

    The 15th International Conference on X-ray Microscopy　2022年6月21日

  16. Hard X-ray Ptychography and Multi-Shot Coherent Diffraction Imaging with Coherent Projection Illumination Optics

    Yuki Takayama, Keizo Fukuda, Motoki Kawashima, Yuki Aoi, Shotaro Oka, Hiroshi Ohno, Tatsuki Akada, Takumi Ikeda, Yasushi Kagoshima

    Materials Research Meeting 2019　2019年12月13日

  17. Hard X-ray ptychography with coherent projection illumination optics 招待有り

    Yuki Takayama, Keizo Fukuda, Motoki Kawashima, Yuki Aoi, Tatsuki Akada, Takumi Ikeda, Yasushi Kagoshima

    The 15th Symposium of Japanese Research Community on X-ray Imaging Optics　2019年10月26日

  18. Practical Designing of Inverse-Phase Composite Zone Plate for Deeper Depth of Focus

    Yasushi Kagoshima, Yuki Takayama

    The 15th Symposium of Japanese Research Community on X-ray Imaging Optics　2019年10月25日

  19. Measurement of horizontal beam emittance of undulator radiation by tandem-double-slit optical system

    Yasushi Kagoshima, Tatsuki Akada, Takumi Ikeda, Motoki Kawashima, Yuki Aoi, Yuki Takayama

    The 15th Symposium of Japanese Research Community on X-ray Imaging Optics　2019年10月25日

  20. X-ray lensless nano-imaging toward investigation of nanostructured materials and devices

    Yuki Takayama, Yasushi Kagoshima, Shotaro Oka, Hiroshi Ohno

    Symposium on bioinspired design of advanced materials　2019年10月10日

  21. The physical properties and structure of wheat noodles (Tenobe Somen) after different storage periods 招待有り

    Natsuki Hosoda, Yuki Takayama, Tatsuki Akada, Yuki Aoi, Nobutake Hara, Miki Yoshimura

    The China-Japan Joint Symposium on Food Hydrocolloids　2019年9月26日

  22. Proposal for Inverse-Phase Composite Zone Plate for Deeper Depth of Focus 国際会議

    Yasushi Kagoshima, Yuki Takayama

    14th International Conference on X-ray Microscopy　2018年8月22日

  23. Cryogenic coherent X-ray diffraction imaging techniques for structural analyses of biological cells and cellular organelles 国際会議

    Amane Kobayashi, Yuki Takayama, Tomotaka Oroguchi, Koji Okajima, Mao Oide, Masaki Yamamoto, Masayoshi Nakasako

    14th International Conference on X-ray Microscopy　2018年8月19日

  24. Coherent X-ray Diffraction Imaging at SPring-8 Hyogo Beamline BL24XU 国際会議

    Yuki Takayama, Yuki Takami, Keizo Fukuda, Takamasa Miyagawa, Yoshimasa Urushihara, Shigeo Kuwamoto, Yasushi Kagoshima

    The 24th Congress of the International Commission for Optics　2017年8月22日

  25. Preparation of Lanthanum Silicate Electrolyte with High Conductivity and High Chemical Stability 国際会議

    Hikaru Hayakawa, Atsushi Mineshige, Takuma Nishimoto, Akie Heguri, Tetsuo Yazawa, Yuki Takayama, Yasushi Kagoshima, Hidekazu Takano, Shingo Takeda, Junji Matsui

    21st International Conference on Solid State Ionics　2017年6月18日

  26. Coherent X-ray Diffraction Imaging at SPring-8 Hyogo Beamline BL24XU 国際会議

    Yuki Takayama, Yuki Takami, Takamasa Miyagawa, Yasushi Kagoshima

    International Conference on X-ray Optics and Application 2017　2017年4月18日

  27. Blue-light induced structural changes of phototropin revealed by small-angle X-ray scattering 国際会議

    Mao Oide, Koji Okajima, Yuki Sekiguchi, Yuki Takayama, Tomotaka Oroguchi, Sachiko Kashojiya, Takaaki Hikima, Masaki Yamamoto, Masayoshi Nakasako

    Okazaki Conference 2017 “Grand Challenges in Small-angle Scattering”　2017年3月18日

  28. Cryogenic coherent diffraction imaging of biological samples with synchrotron and X-ray laser 国際会議

    Yuki Takayama, Masaki Yamamoto, Koji Yonekura, Yuki Sekiguchi, Amane Kobayashi, Koji Okajima, Tomotaka Oroguchi, Masayoshi Nakasako, Yayoi Inui, Sachihiro Matsunaga

    13th International Conference on X-ray Microscopy　2016年8月18日

  29. Cryogenic coherent X-ray diffraction imaging of organelles at SACLA 国際会議

    Yuki Takayama, Koji Yonekura, Masaki Yamamoto, Amane Kobayashi, Yuki Sekiguchi, Tomotaka Oroguchi, Masayoshi Nakasako, Yayoi Inui, Sachihiro Matsunaga

    The 1st SACLA Workshop on Femtosecond Crystallography　2015年3月26日

  30. Coherent X-ray diffraction imaging of chloroplasts from Cyanidioschyzon merolae by using X-ray free electron laser 国際会議

    Yuki Sekiguchi, Amane Kobayashi, Tomotaka Oroguchi, Masayoshi Nakasako, Inui Yayoi, Sachihiro Matsunaga, Yuki Takayama, Masaki Yamamoto

    The 2nd International Symposium on Plant Environmental Sensing　2015年3月14日

  31. Cryogenic coherent X-ray diffraction imaging of biological particles at SACLA 国際会議

    Yuki Takayama, Masayoshi Nakasako, Tomotaka Oroguchi, Yuki Sekiguchi, Masaki Yamamoto, Koji Yonekura, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Tsujimoto-Inui

    23rd Congress and General Assembly of the International Union of Crystallography　2014年8月7日

  32. Single-shot 3D structure determination of nanocrystals with femtosecond X-ray free electron laser pulses 国際会議

    Jianwei Miao, Rui Xu, Huaidong Jiang, Changyong Song, Jose A. Rodriguez, Zhifeng Huang, Chien-Chun Chen, Daewoong Nam, Jaehyun Park, Marcus Gallagher-Jones, Sangsoo Kim, Sunam Kim, Akihiro Suzuki, Yuki Takayama, Tomotaka Oroguchi, Yukio Takahashi, Jiadong Fan, Yunfei Zou, Takaki Hatsui, Yuichi Inubushi, Takashi Kameshima, Koji Yonekura, Kensuke Tono, Tadashi Togashi, Takahiro Sato, Masaki Yamamoto, Masayoshi Nakasako, Makina Yabashi, Tetsuya Ishikawa

    The 6-th International Workshop on FEL Science: New Horizon of XFEL ScienceThe 6-th International Workshop on FEL Science: New Horizon of XFEL Science　2013年11月4日

  33. Cryogenic CXDI experiments for non-crystalline particles using KOTOBUKI-1 apparatus at SACLA: the present status and future plan 国際会議

    Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Keiya Shirahama, Masaki Yamamoto, Takaaki Hikima, Koji Yonekura, Saori Maki-Yonekura, Yoshiki Kohmura, Yuichi Inubushi, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Inui, Yuichi Ichikawa, Hitoshi Kurumizaka, Kensuke Tono, Takashi Kameshima, Yasumasa Joti, Takahiko Hoshi

    The 6-th International Workshop on FEL Science: New Horizon of XFEL Science　2013年11月4日

  34. Image reconstruction from diffraction patterns in coherent X-ray diffraction imaging using the dark-field phase-retrieval method 国際会議

    Amane Kobayashi, Yuki Takayama, Tomotaka Oroguchi, Masayoshi Nakasako

    The 7th Asia Oceania Forum for Synchrotron Radiation Research　2013年9月22日

  35. KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging at SPring-8 and SACLA 国際会議

    Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Keiya Shirahama, Masaki Yamamoto, Takaaki Hikima, Koji Yonekura, Saori Maki-Yonekura, Yoshiki Kohmura, Yuichi Inubushi, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Tsujimoto-Inui, Kensuke Tono, Takashi Kameshima, Yasumasa Joti, Takahiko Hoshi

    The 7th Asia Oceania Forum for Synchrotron Radiation Research　2013年9月22日

  36. Software suite “SHITENNO” for automatically processing diffraction patterns in coherent X-ray diffraction imaging experiments at SACLA 国際会議

    Yuki Sekiguchi, Tomotaka Oroguchi, Yuki Takayama, Masayoshi Nakasako

    The 7th Asia Oceania Forum for Synchrotron Radiation Research　2013年9月22日

  37. Humidity-controlled preparation of frozen-hydrated biological samples for cryogenic coherent X-ray diffraction imaging 国際会議

    Yuki Takayama, Masayoshi Nakasako, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Masaki Yamamoto, Koji Yonekura, Takaaki Hikima, Saori Maki-Yonekura, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Tsujimoto-Inui, Shoichi Kato, Takahiko Hoshi

    11th International Conference on Biology and Synchrotron Radiation　2013年9月11日

  38. Cryogenic CXDI of non-crystalline particles at SACLA 国際会議

    Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Makoto Nakajima, Masaki Yamamoto, Koji Yonekura, Takaaki Hikima, Saori Maki-Yonekura, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Shoichi Kato, Takahiko Hoshi

    4-th Japan-France Joint Seminar　2013年1月9日

  39. Cryogenic CXDI of non-crystalline particles at SACLA 国際会議

    Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Masaki Yamamoto, Koji Yonekura, Takaaki Hikima, Saori Maki-Yonekura, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Shoichi Kato, Takahiko Hoshi

    Coherence 2012 International Workshop on Phase Retrieval and Coherent Scattering　2012年1月20日

  40. Humidity-controlled preparation of frozen-hydrated, non-crystalline particles for cryogenic coherent X-ray diffraction imaging 国際会議

    Yuki Takayama, Masayoshi Nakasako, Tomotaka Oroguchi, Yuki Sekiguchi, Masaki Yamamoto, Koji Yonekura, Takaaki Hikima, Saori Maki-Yonekura, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Shoichi Kato, Takahiko Hoshi

    Coherence 2012 International Workshop on Phase Retrieval and Coherent Scattering　2012年1月18日

  41. Coherent X-ray diffraction microscopy of non-crystalline biological particles at cryogenic temperatures 国際会議

    Masayoshi Nakasako, Masaki Yamamoto, Yuki Takayama, Wataru Kodama, Keita Sakamoto, Yoshinori Nishino, Koji Yonekura, Kazuhiro Maeshima, Keiya Shirahama, Keiichi Namba

    The 3rd Workshop on FEL Science: “Emerging X-ray Applications in Biological Systems-II”　2010年10月5日

  42. Protein crystallography for humidity-controlled crystals 国際会議

    Yuki Takayama, Masayoshi Nakasako

    International Symposium on “Molecular Science for Supra Functional Systems”　2009年6月5日

  43. Structural and informatics studies on plant blue-light receptor phototropin 1 and 2 国際会議

    Masayoshi Nakasako, Yuki Takayama, Aya Iwata, Kazunori Zikihara, Kohji Okajima, Daisuke Matsuoka, Hitomi Katsura, Satoru Tokutomi

    International Symposium on “Molecular Science for Supra Functional Systems”　2009年6月5日

︎全件表示 ︎最初の5件までを表示

共同研究・競争的資金等の研究課題 12

  1. 放射光軟X線光源を活用した軽元素で構成された複合酸化物の局所構造解析手法の開発

    村松 淳司, 高山 裕貴, 日高 將文

    提供機関：Japan Society for the Promotion of Science

    制度名：Grants-in-Aid for Scientific Research

    研究種目：Grant-in-Aid for Scientific Research (A)

    研究機関：Tohoku University

    2024年4月1日 ～ 2029年3月31日

  2. 可視化技術を活用した野菜における生理障害発生機構の解明

    金山 喜則, 高山 裕貴, 栗原 大輔, 加藤 一幾, 西山 学

    2025年4月1日 ～ 2028年3月31日

  3. 次世代放射光X線ナノCT計測の確立と展開

    高山 裕貴

    2022年 ～ 2027年

    詳細を見る 詳細を閉じる

    本研究では、次世代放射光を活用したレンズレスナノCT技術「X線タイコグラフィ」を基盤とし、「深層学習を援用した計測限界突破」と「機械学習による機能発現過程の理解」により、触媒粒子の化学状態から有機/無機材料の空間階層構造まで、ナノからミクロに亘って理解する『次世代放射光X線ナノCT技術』を構築します。社会課題への応用を通じて、計測と予測に基づくものづくり推進のための変革の一翼を担います。

  4. 極低温加工と低温焼もどしによる加工誘起マルテンサイトの多機能化

    土田 紀之, 高山 裕貴

    2023年4月1日 ～ 2026年3月31日

    詳細を見る 詳細を閉じる

    2023年度は，高強度マルテンサイト鋼の機械的特性におよぼす予ひずみの影響を、ストレイン・テンパリング材と，焼入れ・焼もどし材の2種類の試料を用いて調査を行った．ここで，ストレイン・テンパリングとは，焼入れしたマルテンサイト鋼に，数%以下の予ひずみを加え，低温で焼もどし処理を行うことである． ここでは，炭素量が0.4%の鉄鋼材料を用いて，焼入れ処理後に，室温にて0.5%予ひずみを加えたサンプルを473Kで焼もどし処理を行った．このサンプルより引張試験片を作製し，試験温度を室温から573Kまで変化させて引張試験を行った．室温以上で引張試験を行った理由は，引張変形中にセメンタイトが析出する可能性を調査したためである． 296から573Kの変形温度での引張試験において，ストレイン・テンパリングしたサンプルは473から523Kで引張強さと均一伸びの両方が増加したのに対し，焼入れ・焼もどしのサンプルは引張強さと降伏強さはほとんど変化せずに，均一伸びの増加を示した．これらのサンプルについては，J-PARCのビームラインTAKUMIを用いて，引張変形中のその場中性子回折実験を行った．その結果，変形温度が296Kから523Kに上昇するにつれて，bcc相への応力分配が増加することがわかった．この時，セメンタイト相のピークはわずかであり，強度の定量解析は困難であった．焼入れたサンプルに加えた0.5%の予ひずみは，わずかな加工硬化を伴って296Kでの降伏強さを増加させた．この時，プロファイル解析により転位密度を算出した．初期の転位密度は523Kで減少したが，その値は降伏後に著しく増加し，引張強さと均一伸びのより良い組み合わせにつながった．さらに，マルテンサイト鋼の変形前の転位密度の変化と降伏後の転位密度の増加は，予ひずみ温度，焼もどし温度，変形温度の組み合わせによって大きく変化した．

  5. 放射線損傷限界を超える細胞の分子分解能三次元イメージング 競争的資金

    高山 裕貴

    2019年4月 ～ 2022年3月

  6. 有機栽培ケールの構造・栄養学的観点による相関解析に基づいた機能性野菜チップスの開発 競争的資金

    鯛 かおる

    2018年7月 ～ 2019年3月

  7. 栄養学的・構造学的評価による高たんぱく・高栄養の新規スマイルケア食品の開発 競争的資金

    鯛 かおる

    2018年7月 ～ 2019年3月

  8. 材料・細胞試料の階層構造可視化に向けた 広 視野・高分解能 X 線顕微鏡の開発 競争的資金

    高山 裕貴

    2017年4月 ～ 2018年3月

  9. ホログラフィックX線回折イメージング法による単細胞藻類細胞分裂機構の研究 競争的資金

    高山 裕貴

    2016年4月 ～ 2018年3月

  10. コヒーレントX線回折イメージング法とX線結像光学系の融合による高分解能・高精度X線顕微鏡の開発 競争的資金

    高山 裕貴

    2016年7月 ～ 2017年3月

  11. コヒーレントX線回折顕微鏡法による、単細胞真核生物シゾン細胞分裂過程のナノメートル分解能空間階層イメージング 競争的資金

    高山 裕貴

    2013年4月 ～ 2016年3月

  12. コヒーレントX線回折顕微鏡法による真核細胞のナノメートル分解能空間階層構造解析 競争的資金

    高山 裕貴

    2013年8月 ～ 2015年3月

︎全件表示 ︎最初の5件までを表示

担当経験のある科目(授業) 3

  1. 装置実習II (大学院・ビームライン実習)　兵庫県立大学 大学院 理学研究科

  2. 英語科学問題演習(2年次)　兵庫県立大学理学部

  3. 科学英語(4年次)　兵庫県立大学理学部