Details of the Researcher

PHOTO

Yuki Takayama
Section
International Center for Synchrotron Radiation Innovation Smart
Job title
Associate Professor
Degree

  • 博士（理学）（慶應義塾大学）

  • 修士（理学）（慶應義塾大学）

e-Rad No.
40710132

Research History 4

  • 2022/10 - Present
    Tohoku University　International Center for Synchrotron Radiation Innovation Smart Innovation and Technology Transfer Division

  • 2021/04 - 2022/09
    University of Hyogo　Graduate School of Science　Assistant Professor

  • 2016/04 - 2021/03
    University of Hyogo　Graduate School of Material Science　Assistant Professor

  • 2013/04 - 2016/03
    RIKEN　SPring-8 Center　Special Postdoctoral Researcher

Education 3

  • Keio Univerisity　Graduate School of Science and Technology　School of Fundamental Science and Technology

    2010/04 - 2013/03

  • Keio Univerisity　Graduate School of Science and Technology　School of Fundamental Science and Technology

    2008/04 - 2010/03

  • Keio Univerisity　Faculty of Science and Technology　Department of Physics

    2004/04 - 2008/03

Professional Memberships 4

  • 農芸化学会

    2024/05 - Present

  • THE CRYSTALLOGRAPHIC SOCIETY OF JAPAN

  • THE BIOPHYSICAL SOCIETY OF JAPAN

  • THE JAPANESE SOCIETY FOR SYNCHROTRON RADIATION RESEARCH

Research Interests 4

  • タイコグラフィー

  • Biophysics

  • X-ray microscope

  • Cohrerent diffraction imaging

Research Areas 1

  • Natural sciences / Bio-, chemical, and soft-matter physics /

Awards 8

  1. 第20回SPring-8産業利用報告会 ポスター発表の部 優秀発表賞

    2023/11　SPring-8利用推進協議会　SAXSイメージングモデルを用いたシミュレーションによる手延べ素麺の食感解析

  2. 日本結晶学会賞進歩賞

    2023/07　一般社団法人 日本結晶学会　マルチショット・コヒーレントX線回折イメージングの開発と実用化

  3. 2023年春期講演大会 第40回ポスターセッション 優秀ポスター賞

    2023/03　公益社団法人日本金属学会　in situ 2D XAFS と機械学習による材料腐食メカニズム推定

  4. 第19回SPring-8産業利用報告会 ポスター発表の部 優秀発表賞

    2022/11　SPring-8利用推進協議会　手延べ製法の条件と手延べ素麺の美味しさ評価

  5. 優秀論文発表賞A

    2022/02　一般社団法人 電気学会　コヒーレントX線を用いたレンズレス時空間階層イメージング法の開発

  6. SPRUC 2021 Young Scientist Award

    2021/07　SPring-8 Users Community　Development of lens-less nanoimaging method using coherent X-rays for visualization of spatiotemporally hierarchical structures in materials and biological sciences

  7. 第16回SPring-8産業利用報告会 口頭発表の部 優秀発表賞

    2019/11　SPring-8利用推進協議会　真空フライによるケールの高機能化と構造・機能性・おいしさ評価

  8. 理工学研究科 藤原賞

    2013/03　慶應義塾大学　湿度制御技術開発を軸とした細胞内空間階層構造のX線回折イメージング

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Papers 56

  1. 放射光コヒーレントX線による先端ナノイメージング技術 －腐食防食研究応用への期待－ Invited Peer-reviewed

    高山裕貴

    表面技術　75　(10)　424-429　2024/10

  2. Ptychographic phase retrieval via a deep-learning-assisted iterative algorithm Peer-reviewed

    Koki Yamada, Natsuki Akaishi, Kohei Yatabe, Yuki Takayama

    Journal of Applied Crystallography　57　(5)　1323-1335　2024/08/19

    Publisher: International Union of Crystallography (IUCr)

    DOI： 10.1107/s1600576724006897 　

    eISSN： 1600-5767

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    Ptychography is a powerful computational imaging technique with microscopic imaging capability and adaptability to various specimens. To obtain an imaging result, it requires a phase-retrieval algorithm whose performance directly determines the imaging quality. Recently, deep neural network (DNN)-based phase retrieval has been proposed to improve the imaging quality from the ordinary model-based iterative algorithms. However, the DNN-based methods have some limitations because of the sensitivity to changes in experimental conditions and the difficulty of collecting enough measured specimen images for training the DNN. To overcome these limitations, a ptychographic phase-retrieval algorithm that combines model-based and DNN-based approaches is proposed. This method exploits a DNN-based denoiser to assist an iterative algorithm like ePIE in finding better reconstruction images. This combination of DNN and iterative algorithms allows the measurement model to be explicitly incorporated into the DNN-based approach, improving its robustness to changes in experimental conditions. Furthermore, to circumvent the difficulty of collecting the training data, it is proposed that the DNN-based denoiser be trained without using actual measured specimen images but using a formula-driven supervised approach that systemically generates synthetic images. In experiments using simulation based on a hard X-ray ptychographic measurement system, the imaging capability of the proposed method was evaluated by comparing it with ePIE and rPIE. These results demonstrated that the proposed method was able to reconstruct higher-spatial-resolution images with half the number of iterations required by ePIE and rPIE, even for data with low illumination intensity. Also, the proposed method was shown to be robust to its hyperparameters. In addition, the proposed method was applied to ptychographic datasets of a Simens star chart and ink toner particles measured at SPring-8 BL24XU, which confirmed that it can successfully reconstruct images from measurement scans with a lower overlap ratio of the illumination regions than is required by ePIE and rPIE.

  3. Subgradient-projection-based stable phase-retrieval algorithm for X-ray ptychography

    Natsuki Akaishi, Koki Yamada, Kohei Yatabe, Yuki Takayama

    Journal of Applied Crystallography　57　(Pt 4)　1085-1097　2024/08/01

    DOI： 10.1107/S1600576724004709 　

    ISSN： 0021-8898

    eISSN： 1600-5767

  4. Development and application of multiple-shot coherent x-ray diffraction imaging Invited Peer-reviewed

    66　(2)　94-102　2024/05/31

    Publisher: The Crystallographic Society of Japan

    DOI： 10.5940/jcrsj.66.94 　

    ISSN： 0369-4585

    eISSN： 1884-5576

  5. Towards sub-10 nm spatial resolution by tender X-ray ptychographic coherent diffraction imaging

    Nozomu Ishiguro, Fusae Kaneko, Masaki Abe, Yuki Takayama, Junya Yoshida, Taiki Hoshino, Shuntaro Takazawa, Hideshi Uematsu, Yuhei Sasaki, Naru Okawa, Keichi Takahashi, Hiroyuki Takizawa, Hiroyuki Kishimoto, Yukio Takahashi

    Applied Physics Express　17　(5)　2024/05/01

    DOI： 10.35848/1882-0786/ad4846 　

    ISSN： 1882-0778

    eISSN： 1882-0786

  6. Redox-induced changes in nanostructures and electron densities of connected Pt–Fe catalysts for fuel cells revealed via in situ coherent X-ray diffraction Peer-reviewed

    Yuki Takayama, Takanori Itoh, Hideto Imai, Hidenori Kuroki, Takeo Yamaguchi

    Japanese Journal of Applied Physics　63　(4)　048002　2024/03/21

    Publisher: IOP Publishing

    DOI： 10.35848/1347-4065/ad36a0 　

    ISSN： 0021-4922

    eISSN： 1347-4065

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    Abstract In a sustainable society, polymer electrolyte fuel cells are one of the promising energy devices for automobiles and residential uses because of their high energy efficiency. On the other hand, the current cathode catalysts are mainly made of precious metals such as Pt, and their activity and durability have become an issue for their widespread use. Carbon-free, connected Pt–Fe-alloy catalysts have been developed to solve this problem. However, their catalytic mechanism has not yet been clarified. In this study, redox-induced changes in the nanostructures and electron density distributions of the catalyst were investigated via coherent X-ray diffraction imaging.

  7. Noise tolerance of a sub-pixel shift method for upsampling diffraction patterns in coherent X-ray diffraction imaging Peer-reviewed

    Yuki Takayama, Yasushi Kagoshima

    Japanese Journal of Applied Physics　63　(3)　038001　2024/03/01

    Publisher: IOP Publishing

    DOI： 10.35848/1347-4065/ad2b1a 　

    ISSN： 0021-4922

    eISSN： 1347-4065

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    Abstract In coherent X-ray diffraction imaging, speckles on a coherent diffraction pattern must be sampled at intervals sufficiently finer than the Nyquist interval, which imposes an upper limit on the sample size. To overcome the size limitation, a sub-pixel shift method for upsampling coherent diffraction patterns was proposed. This paper reports on the evaluation of the noise tolerance of the upsampling algorithm by a simulation. The quality of the images reconstructed from the upsampled diffraction pattern and pattern recorded by a detector with an equivalent pixel size was comparable when the optimum number of upsampling iterations is adopted.

  8. Protocol using similarity score and improved shrink-wrap algorithm for better convergence of phase-retrieval calculation in X-ray diffraction imaging Peer-reviewed

    Syouyo Yoshida, Kosei Harada, So Uezu, Yuki Takayama, Masayoshi Nakasako

    Journal of Synchrotron Radiation　31　(1)　113-128　2024/01/01

    Publisher: International Union of Crystallography (IUCr)

    DOI： 10.1107/s1600577523009864 　

    eISSN： 1600-5775

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    In X-ray diffraction imaging (XDI), electron density maps of a targeted particle are reconstructed computationally from the diffraction pattern alone using phase-retrieval (PR) algorithms. However, the PR calculations sometimes fail to yield realistic electron density maps that approximate the structure of the particle. This occurs due to the absence of structure amplitudes at and near the zero-scattering angle and the presence of Poisson noise in weak diffraction patterns. Consequently, the PR calculation becomes a bottleneck for XDI structure analyses. Here, a protocol to efficiently yield realistic maps is proposed. The protocol is based on the empirical observation that realistic maps tend to yield low similarity scores, as suggested in our prior study [Sekiguchi et al. (2017), J. Synchrotron Rad.24, 1024–1038]. Among independently and concurrently executed PR calculations, the protocol modifies all maps using the electron-density maps exhibiting low similarity scores. This approach, along with a new protocol for estimating particle shape, improved the probability of obtaining realistic maps for diffraction patterns from various aggregates of colloidal gold particles, as compared with PR calculations performed without the protocol. Consequently, the protocol has the potential to reduce computational costs in PR calculations and enable efficient XDI structure analysis of non-crystalline particles using synchrotron X-rays and X-ray free-electron laser pulses.

  9. Similarity score for screening phase-retrieved maps in X-ray diffraction imaging – characterization in reciprocal space Peer-reviewed

    Yuki Takayama, Masayoshi Nakasako

    Journal of Synchrotron Radiation　31　(1)　95-112　2024/01/01

    Publisher: International Union of Crystallography (IUCr)

    DOI： 10.1107/s1600577523009827 　

    eISSN： 1600-5775

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    X-ray diffraction imaging (XDI) is utilized for visualizing the structures of non-crystalline particles in material sciences and biology. In the structural analysis, phase-retrieval (PR) algorithms are applied to the diffraction amplitude data alone to reconstruct the electron density map of a specimen particle projected along the direction of the incident X-rays. However, PR calculations may not lead to good convergence because of a lack of diffraction patterns in small-angle regions and Poisson noise in X-ray detection. Therefore, the PR calculation is still a bottleneck for the efficient application of XDI in the structural analyses of non-crystalline particles. For screening maps from hundreds of trial PR calculations, we have been using a score and measuring the similarity between a pair of retrieved maps. Empirically, probable maps approximating the particle structures gave a score smaller than a threshold value, but the reasons for the effectiveness of the score are still unclear. In this study, the score is characterized in terms of the phase differences between the structure factors of the retrieved maps, the usefulness of the score in screening the maps retrieved from experimental diffraction patterns is demonstrated, and the effective resolution of similarity-score-selected maps is discussed.

  10. Ultrastructure and fractal property of chromosomes in close-to-native yeast nuclei visualized using X-ray laser diffraction Peer-reviewed

    So Uezu, Takahiro Yamamoto, Mao Oide, Yuki Takayama, Koji Okajima, Amane Kobayashi, Masaki Yamamoto, Masayoshi Nakasako

    Scientific Reports　13　(1)　2023/07/05

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-023-37733-6 　

    eISSN： 2045-2322

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    Abstract Genome compaction and activity in the nucleus depend on spatiotemporal changes in the organization of chromatins in chromosomes. However, the direct imaging of the chromosome structures in the nuclei has been difficult and challenging. Herein, we directly visualized the structure of chromosomes in frozen-hydrated nuclei of budding yeast in the interphase using X-ray laser diffraction. The reconstructed projection electron density maps revealed inhomogeneous distributions of chromosomes, such as a 300 nm assembly and fibrous substructures in the elliptic-circular shaped nuclei of approximately 800 nm. In addition, from the diffraction patterns, we confirmed the absence of regular arrangements of chromosomes and chromatins with 400–20 nm spacing, and demonstrated that chromosomes were composed of self-similarly assembled substructural domains with an average radius of gyration of 58 nm and smooth surfaces. Based on these analyses, we constructed putative models to discuss the organization of 16 chromosomes, carrying DNA of 4.1 mm in 800 nm ellipsoid of the nucleus at the interphase. We anticipate the structural parameters on the fractal property of chromosomes and the experimental images to be a starting point for constructing more sophisticated 3D structural models of the nucleus.

  11. Ｘ線タイコグラフィによるゴム中ナノフィラー構造観察 Peer-reviewed

    砂川 正典, 山本 渥史, 岡本 泰志, 高山 裕貴

    SPring-8/SACLA利用研究成果集　11　(2)　153-155　2023/04

  12. 兵庫県産黒大豆のミネラル及びポリフェノール類に着目した成分分析と機能性 Peer-reviewed

    吉村美紀, 加藤陽二, 和田裕子, 島田良子, 小林美幸, 赤松成基, 鯛かおる, 高山裕貴

    兵庫県立大学環境人間学部研究報告　25　41-46　2023/03

  13. Practical design of the inverse-phase composite zone plate for improved depth of focus

    Yasushi Kagoshima, Yuki Takayama

    PROCEEDINGS OF THE 15TH INTERNATIONAL CONFERENCE ON X-RAY MICROSCOPY – XRM2022　2023

    Publisher: AIP Publishing

    DOI： 10.1063/5.0168281 　

    ISSN： 0094-243X

  14. Phase retrieval based on a total-variation-regularized Poisson model for X-ray ptychographic imaging of low-contrast objects Peer-reviewed

    Kohei Yatabe, Yuki Takayama

    Journal of Applied Crystallography　55　(4)　978-992　2022/08

    Publisher: International Union of Crystallography (IUCr)

    DOI： 10.1107/s1600576722005234 　

    ISSN： 0021-8898

    eISSN： 1600-5767

  15. Development of Coherent X-ray Diffraction Imaging at SPring-8 Invited Peer-reviewed

    Yuki TAKAYAMA, Masayoshi NAKASAKO

    Nihon Kessho Gakkaishi　64　(1)　41-49　2022/02/28

    Publisher: The Crystallographic Society of Japan

    DOI： 10.5940/jcrsj.64.41 　

    ISSN： 0369-4585

    eISSN： 1884-5576

  16. Dynamic nanoimaging of extended objects via hard X-ray multiple-shot coherent diffraction with projection illumination optics Peer-reviewed

    Yuki Takayama, Keizo Fukuda, Motoki Kawashima, Yuki Aoi, Daiki Shigematsu, Tatsuki Akada, Takumi Ikeda, Yasushi Kagoshima

    Communications Physics　4　(1)　2021/12

    Publisher: Springer Science and Business Media {LLC}

    DOI： 10.1038/s42005-021-00539-x 　

    eISSN： 2399-3650

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    <title>Abstract</title>The quest for understanding the structural mechanisms of material properties and biological cell functions has led to the active development of coherent diffraction imaging (CDI) and its variants in the hard X-ray regime. Herein, we propose multiple-shot CDI, a full-field CDI technique dedicated to the visualisation of local nanostructural dynamics in extended objects at a spatio-temporal resolution beyond that of current instrumentation limitations. Multiple-shot CDI reconstructs a “movie” of local dynamics from time-evolving diffraction patterns, which is compatible with a robust scanning variant, ptychography. We developed projection illumination optics to produce a probe with a well-defined illumination area and a phase retrieval algorithm, establishing a spatio-temporal smoothness constraint for the reliable reconstruction of dynamic images. The numerical simulations and proof-of-concept experiment using synchrotron hard X-rays demonstrated the capability of visualising a dynamic nanostructured object at a frame rate of 10 Hz or higher.

  17. Optical transfer function of inverse-phase composite zone plate devised for deep-focusing X-ray microscopes Peer-reviewed

    Yasushi Kagoshima, Yuki Takayama

    Japanese Journal of Applied Physics　60　(11)　118001-118001　2021/11/01

    Publisher: IOP Publishing

    DOI： 10.35848/1347-4065/ac2e69 　

    ISSN： 0021-4922

    eISSN： 1347-4065

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    Abstract The optical transfer function (OTF) of an inverse-phase composite zone plate under incoherent illumination was numerically evaluated by convoluting the point spread function (PSF) and transmission distribution of a model sample with gradually changing spatial frequency. The PSF used in the simulation was obtained from our previous study on deep-focusing X-ray microscopes. As expected, the contrast in the sample image was lesser than that produced by a conventional zone plate. Phase-reversed contrast—spurious resolution—appeared at a high spatial frequency. Although the calculated OTF is slightly aberrated, its deep-focusing capability is advantageous for X-ray microimaging of thick samples.

  18. Three-dimensional Observation of Automotive Coating Film by Synchrotron Radiation X-ray Ptychography Peer-reviewed

    Journal of Japan Coating Technology Association　56　(6)　204-210　2021/06/30

  19. Common architectures in cyanobacteria Prochlorococcus cells visualized by X-ray diffraction imaging using X-ray free electron laser. International-journal Peer-reviewed

    Amane Kobayashi, Yuki Takayama, Takeshi Hirakawa, Koji Okajima, Mao Oide, Tomotaka Oroguchi, Yayoi Inui, Masaki Yamamoto, Sachihiro Matsunaga, Masayoshi Nakasako

    Scientific reports　11　(1)　3877-3877　2021/02/16

    DOI： 10.1038/s41598-021-83401-y 　

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    Visualization of intracellular structures and their spatial organization inside cells without any modification is essential to understand the mechanisms underlying the biological functions of cells. Here, we investigated the intracellular structure of cyanobacteria Prochlorococcus in the interphase by X-ray diffraction imaging using X-ray free-electron laser. A number of diffraction patterns from single cells smaller than 1 µm in size were collected with high signal-to-noise ratio with a resolution of up to 30 nm. From diffraction patterns, a set of electron density maps projected along the direction of the incident X-ray were retrieved with high reliability. The most characteristic structure found to be common among the cells was a C-shaped arrangement of 100-nm sized high-density spots, which surrounded a low-density area of 100 nm. Furthermore, a three-dimensional map reconstructed from the projection maps of individual cells was non-uniform, indicating the presence of common structures among cyanobacteria cells in the interphase. By referring to the fluorescent images for distributions of thylakoid membranes, nucleoids, and carboxysomes, we inferred and represented their spatial arrangements in the three-dimensional map. The arrangement allowed us to discuss the relevance of the intracellular organization to the biological functions of cyanobacteria.

  20. Lanthanum silicate-based layered electrolyte for intermediate-temperature fuel cell application Peer-reviewed

    Atsushi Mineshige, Atsushi Saito, Mio Kobayashi, Hikaru Hayakawa, Mizuki Momai, Tetsuo Yazawa, Hideki Yoshioka, Mitsumasa Sakao, Ryohei Mori, Yuki Takayama, Yasushi Kagoshima, Junji Matsui

    Journal of Power Sources　475　2020/11/01

    DOI： 10.1016/j.jpowsour.2020.228543 　

    ISSN： 0378-7753

  21. Domain Organization in Plant Blue-Light Receptor Phototropin2 of Arabidopsis thaliana Studied by Small-Angle X-ray Scattering. International-journal Peer-reviewed

    Masayoshi Nakasako, Mao Oide, Yuki Takayama, Tomotaka Oroguchi, Koji Okajima

    International journal of molecular sciences　21　(18)　2020/09/10

    DOI： 10.3390/ijms21186638 　

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    Phototropin2 (phot2) is a blue-light (BL) receptor protein that regulates the BL-dependent activities of plants for efficient photosynthesis. Phot2 is composed of two light-oxygen-voltage sensing domains (LOV1 and LOV2) to absorb BL, and a kinase domain. Photo-activated LOV domains, especially LOV2, play a major role in photo-dependent increase in the phosphorylation activity of the kinase domain. The atomic details of the overall structure of phot2 and the intramolecular mechanism to convert BL energy to a phosphorylation signal remain unknown. We performed structural studies on the LOV fragments LOV1, LOV2, LOV2-linker, and LOV2-kinase, and full-length phot2, using small-angle X-ray scattering (SAXS). The aim of the study was to understand structural changes under BL irradiation and discuss the molecular mechanism that enhance the phosphorylation activity under BL. SAXS is a suitable technique for visualizing molecular structures of proteins in solution at low resolution and is advantageous for monitoring their structural changes in the presence of external physical and/or chemical stimuli. Structural parameters and molecular models of the recombinant specimens were obtained from SAXS profiles in the dark, under BL irradiation, and after dark reversion. LOV1, LOV2, and LOV2-linker fragments displayed minimal structural changes. However, BL-induced rearrangements of functional domains were noted for LOV2-kinase and full-length phot2. Based on the molecular model together with the absorption measurements and biochemical assays, we discuss the intramolecular interactions and domain motions necessary for BL-enhanced phosphorylation activity of phot2.

  22. Measurement of the horizontal beam emittance of undulator radiation by tandem-double-slit optical system. International-journal Peer-reviewed

    Yasushi Kagoshima, Tatsuki Akada, Takumi Ikeda, Motoki Kawashima, Yuki Aoi, Yuki Takayama

    Journal of synchrotron radiation　27　(Pt 3)　799-803　2020/05/01

    DOI： 10.1107/S1600577520004415 　

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    A tandem-double-slit optical system was constructed to evaluate the practical beam emittance of undulator radiation. The optical system was a combination of an upstream slit (S1) and downstream slit (S2) aligned on the optical axis with an appropriate separation. The intensity distribution after the double slits, I(x1, x2), was measured by scanning S1 and S2 in the horizontal direction. Coordinates having 1/\sqrt e intensity were extracted from I(x1, x2), whose contour provided the standard deviation ellipse in the x1-x2 space. I(x1, x2) was converted to the corresponding distribution in the phase space, I(x1, x1'). The horizontal beam emittance was evaluated to be 3.1 nm rad, which was larger than the value of 2.4 nm rad estimated by using ray-tracing. It was found that the increase was mainly due to an increase in beam divergence rather than size.

  23. Methods and application of coherent X-ray diffraction imaging of noncrystalline particles. International-journal Peer-reviewed

    Masayoshi Nakasako, Amane Kobayashi, Yuki Takayama, Kenta Asakura, Mao Oide, Koji Okajima, Tomotaka Oroguchi, Masaki Yamamoto

    Biophysical reviews　12　(2)　541-567　2020/04

    DOI： 10.1007/s12551-020-00690-9 　

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    Microscopic imaging techniques have been developed to visualize events occurring in biological cells. Coherent X-ray diffraction imaging is one of the techniques applicable to structural analyses of cells and organelles, which have never been crystallized. In the experiment, a single noncrystalline particle is illuminated by an X-ray beam with almost complete spatial coherence. The structure of the particle projected along the direction of the beam is, in principle, retrieved from a finely recorded diffraction pattern alone by using iterative phase-retrieval algorithms. Here, we describe fundamental theory and experimental methods of coherent X-ray diffraction imaging and the recent application in structural studies of noncrystalline specimens by using X-rays available at Super Photon Ring of 8-Gev and SPring-8 Angstrom Compact Free Electron Laser in Japan.

  24. 特別企画 試料環境シリーズ(7) 第4回「温度」 4-1 蛋白質結晶の低温X線回折実験 ～低温 1～ Invited Peer-reviewed

    中迫雅由, 高山裕貴, 山本雅貴

    放射光　33　(2)　101-111　2020/01

  25. 貯蔵期間の異なる手延そうめんの性状と構造観察 Peer-reviewed

    細田捺希, 高山裕貴, 赤田樹, 青井雄幹, 原信岳, 吉村美紀

    日本調理科学会誌　52　(6)　386-394　2019/12

  26. Inverse-phase composite zone plate providing deeper focus than the normal diffraction-limited depth of X-ray microbeams. International-journal Peer-reviewed

    Yasushi Kagoshima, Yuki Takayama

    Journal of synchrotron radiation　26　(Pt 1)　52-58　2019/01/01

    DOI： 10.1107/S1600577518016703 　

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    A novel type of zone plate (ZP), termed an inverse-phase composite ZP, is proposed to gain a deeper focus than the standard diffraction-limited depth of focus, with little reduction in spatial resolution. The structure is a combination of an inner ZP functioning as a conventional phase ZP and an outer ZP functioning with third-order diffraction with opposite phase to the inner ZP. Two-dimensional complex amplitude distributions neighboring the focal point were calculated using a wave-optical approach of diffraction integration with a monochromatic plane-wave illumination, where one dimension is the radial direction and the other dimension is the optical-axis direction. The depth of focus and the spatial resolution were examined as the main focusing properties. Two characteristic promising cases regarding the depth of focus were found: a pit-intensity focus with the deepest depth of focus, and a flat-intensity focus with deeper depth of focus than usual ZPs. It was found that twice the depth of focus could be expected with little reduction in the spatial resolution for 10 keV X-ray energy, tantalum zone material, 84 nm minimum fabrication zone width, and zone thickness of 2.645 µm. It was also found that the depth of focus and the spatial resolution were almost unchanged in the photon energy range from 8 to 12 keV. The inverse-phase composite ZP has high potential for use in analysis of practical thick samples in X-ray microbeam applications.

  27. Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation Peer-reviewed

    Kobayashi A, Takayama Y, Okajima K, Oide M, Yamamoto T, Sekiguchi Y, Oroguchi T, Nakasako M, Kohmura Y, Yamamoto M, Hoshi T, Torizuka Y

    Journal of Synchrotron Radiation　25　(6)　1803-1818　2018/11

    DOI： 10.1107/S1600577518012687 　

  28. Proposal for Inverse-Phase Composite Zone Plate for Deeper Depth of Focus Peer-reviewed

    Kagoshima Y, Takayama Y

    Microscopy and Microanalysis　24　(S2)　278-279　2018/08

    DOI： 10.1017/S1431927618013739 　

  29. Cryogenic Coherent X-ray Diffraction Imaging Techniques for Structural Analyses of Biological Cells and Cellular Organelles Peer-reviewed

    Kobayashi A, Takayama Y, Oroguchi T, Okajima K, Oide M, Yamamoto M, Nakasako M

    Microscopy and Microanalysis　24　(S2)　14-15　2018/08

    DOI： 10.1017/S1431927618012503 　

  30. Atmospheric coherent X-ray diffraction imaging for in situ structural analysis at SPring-8 Hyogo beamline BL24XU. International-journal Peer-reviewed

    Yuki Takayama, Yuki Takami, Keizo Fukuda, Takamasa Miyagawa, Yasushi Kagoshima

    Journal of synchrotron radiation　25　(Pt 4)　1229-1237　2018/07/01

    DOI： 10.1107/S1600577518006410 　

    ISSN： 0909-0495

    More details Close

    Coherent X-ray diffraction imaging (CXDI) is a promising technique for non-destructive structural analysis of micrometre-sized non-crystalline samples at nanometre resolutions. This article describes an atmospheric CXDI system developed at SPring-8 Hyogo beamline BL24XU for in situ structural analysis and designed for experiments at a photon energy of 8 keV. This relatively high X-ray energy enables experiments to be conducted under ambient atmospheric conditions, which is advantageous for the visualization of samples in native states. The illumination condition with pinhole-slit optics is optimized according to wave propagation calculations based on the Fresnel-Kirchhoff diffraction formula so that the sample is irradiated by X-rays with a plane wavefront and high photon flux of ∼1 × 1010 photons/16 µmø(FWHM)/s. This work demonstrates the imaging performance of the atmospheric CXDI system by visualizing internal voids of sub-micrometre-sized colloidal gold particles at a resolution of 29.1 nm. A CXDI experiment with a single macroporous silica particle under controlled humidity was also performed by installing a home-made humidity control device in the system. The in situ observation of changes in diffraction patterns according to humidity variation and reconstruction of projected electron-density maps at 5.2% RH (relative humidity) and 82.6% RH at resolutions of 133 and 217 nm, respectively, were accomplished.

  31. Preparation of lanthanum silicate electrolyte with high conductivity and high chemical stability Peer-reviewed

    Atsushi Mineshige, Hikaru Hayakawa, Takuma Nishimoto, Akie Heguri, Tetsuo Yazawa, Yuki Takayama, Yasushi Kagoshima, Hidekazu Takano, Shingo Takeda, Junji Matsui

    Solid State Ionics　319　223-227　2018/06

    DOI： 10.1016/j.ssi.2018.02.002 　

    ISSN： 0167-2738

  32. X-ray diffraction imaging of biological cells at low temperature Invited Peer-reviewed

    中迫 雅由, 高山 裕貴, 小林 周, 山本 隆寛, 大出 真央, 岡島 公司, 苙口 友隆, 山本 雅貴

    放射光 : 日本放射光学会誌 = Journal of the Japanese Society for Synchrotron Radiation Research　31　(3)　189-201　2018/05

    Publisher: 日本放射光学会

    ISSN： 0914-9287

  33. Common structural features of toxic intermediates from alpha-synuclein and GroES fibrillogenesis detected using cryogenic coherent X-ray diffraction imaging Peer-reviewed

    Hiroshi Kameda, Sayaka Usugi, Mana Kobayashi, Naoya Fukui, Seki Lee, Kunihiro Hongo, Tomohiro Mizobata, Yuki Sekiguchi, Yu Masaki, Amane Kobayashi, Tomotaka Oroguchi, Masayoshi Nakasako, Yuki Takayama, Masaki Yamamoto, Yasushi Kawata

    JOURNAL OF BIOCHEMISTRY　161　(1)　55-65　2017/01

    DOI： 10.1093/jb/mvw052 　

    ISSN： 0021-924X

    eISSN： 1756-2651

  34. Blue Light-excited Light-Oxygen-Voltage-sensing Domain 2 (LOV2) Triggers a Rearrangement of the Kinase Domain to Induce Phosphorylation Activity in Arabidopsis Phototropin1 Peer-reviewed

    Mao Oide, Koji Okajima, Sachiko Kashojiya, Yuki Takayama, Tomotaka Oroguchi, Takaaki Hikima, Masaki Yamamoto, Masayoshi Nakasako

    JOURNAL OF BIOLOGICAL CHEMISTRY　291　(38)　19975-19984　2016/09

    DOI： 10.1074/jbc.M116.735787 　

    ISSN： 0021-9258

    eISSN： 1083-351X

  35. TAKASAGO-6 apparatus for cryogenic coherent X-ray diffraction imaging of biological non-crystalline particles using X-ray free electron laser at SACLA Peer-reviewed

    Amane Kobayashi, Yuki Sekiguchi, Yuki Takayama, Tomotaka Oroguchi, Keiya Shirahama, Yasufumi Torizuka, Masahiro Manoda, Masayoshi Nakasako, Masaki Yamamoto

    REVIEW OF SCIENTIFIC INSTRUMENTS　87　(5)　053109　2016/06

    DOI： 10.1063/1.4948317 　

    ISSN： 0034-6748

    eISSN： 1089-7623

  36. Cryogenic coherent X-ray diffraction imaging of biological samples at SACLA: a correlative approach with cryo-electron and light microscopy Invited Peer-reviewed

    Yuki Takayama, Koji Yonekura

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES　72　(Pt 2)　179-189　2016/03

    DOI： 10.1107/S2053273315023980 　

    ISSN： 2053-2733

  37. 金コロイド粒子を利用した非結晶生体試料の高分解能・高精度コヒーレントX線回折イメージング Invited Peer-reviewed

    高山裕貴, 眞木さおり, 苙口友隆, 中迫雅由, 米倉功治

    Isotope News　739　(11)　9-14　2015/11

  38. Cryogenic coherent x-ray diffraction imaging for biological non-crystalline particles using the KOTOBUKI-1 diffraction apparatus at SACLA Peer-reviewed

    Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Yu Masaki, Asahi Fukuda, Saki Hashimoto, Masayoshi Nakasako, Yuichi Ichikawa, Hitoshi Kurumizaka, Mitsuhiro Shimizu, Yayoi Inui, Sachihiro Matsunaga, Takayuki Kato, Keiichi Namba, Keiichi Yamaguchi, Kazuo Kuwata, Hiroshi Kameda, Naoya Fukui, Yasushi Kawata, Takashi Kameshima, Yuki Takayama, Koji Yonekura, Masaki Yamamoto

    JOURNAL OF PHYSICS B-ATOMIC MOLECULAR AND OPTICAL PHYSICS　48　(18)　184003　2015/09

    DOI： 10.1088/0953-4075/48/18/184003 　

    ISSN： 0953-4075

    eISSN： 1361-6455

  39. Coherent X-Ray Diffraction Imaging of Chloroplasts from Cyanidioschyzon merolae by Using X-Ray Free Electron Laser Peer-reviewed

    Yuki Takayama, Yayoi Inui, Yuki Sekiguchi, Amane Kobayashi, Tomotaka Oroguchi, Masaki Yamamoto, Sachihiro Matsunaga, Masayoshi Nakasako

    PLANT AND CELL PHYSIOLOGY　56　(7)　1272-1286　2015/07

    DOI： 10.1093/pcp/pcv032 　

    ISSN： 0032-0781

    eISSN： 1471-9053

  40. Signal enhancement and Patterson-search phasing for high-spatial-resolution coherent X-ray diffraction imaging of biological objects Peer-reviewed

    Yuki Takayama, Saori Maki-Yonekura, Tomotaka Oroguchi, Masayoshi Nakasako, Koji Yonekura

    SCIENTIFIC REPORTS　5　8074　2015/01

    DOI： 10.1038/srep08074 　

    ISSN： 2045-2322

  41. IDATEN and G-SITENNO: GUI-assisted software for coherent X-ray diffraction imaging experiments and data analyses at SACLA Peer-reviewed

    Yuki Sekiguchi, Masaki Yamamoto, Tomotaka Oroguchi, Yuki Takayama, Shigeyuki Suzuki, Masayoshi Nakasako

    Journal of Synchrotron Radiation　21　(6)　1378-1383　2014/11/01

    Publisher: International Union of Crystallography

    DOI： 10.1107/S1600577514017111 　

    ISSN： 1600-5775 0909-0495

  42. Dark-field phase retrieval under the constraint of the Friedel symmetry in coherent X-ray diffraction imaging Peer-reviewed

    Amane Kobayashi, Yuki Sekiguchi, Yuki Takayama, Tomotaka Oroguchi, Masayoshi Nakasako

    OPTICS EXPRESS　22　(23)　27892-27909　2014/11

    DOI： 10.1364/OE.22.027892 　

    ISSN： 1094-4087

  43. Single-shot three-dimensional structure determination of nanocrystals with femtosecond X-ray free-electron laser pulses Peer-reviewed

    Rui Xu, Huaidong Jiang, Changyong Song, Jose A. Rodriguez, Zhifeng Huang, Chien-Chun Chen, Daewoong Nam, Jaehyun Park, Marcus Gallagher-Jones, Sangsoo Kim, Sunam Kim, Akihiro Suzuki, Yuki Takayama, Tomotaka Oroguchi, Yukio Takahashi, Jiadong Fan, Yunfei Zou, Takaki Hatsui, Yuichi Inubushi, Takashi Kameshima, Koji Yonekura, Kensuke Tono, Tadashi Togashi, Takahiro Sato, Masaki Yamamoto, Masayoshi Nakasako, Makina Yabashi, Tetsuya Ishikawa, Jianwei Miao

    NATURE COMMUNICATIONS　5　4061　2014/06

    DOI： 10.1038/ncomms5061 　

    ISSN： 2041-1723

  44. Data processing software suite SITENNO for coherent X-ray diffraction imaging using the X-ray free-electron laser SACLA Peer-reviewed

    Yuki Sekiguchi, Tomotaka Oroguchi, Yuki Takayama, Masayoshi Nakasako

    JOURNAL OF SYNCHROTRON RADIATION　21　(Pt 3)　600-612　2014/05

    DOI： 10.1107/S1600577514003439 　

    ISSN： 0909-0495

    eISSN： 1600-5775

  45. Coherent X-ray Diffraction Imaging of Non-Crystalline Particles using X-ray Free Electron Laser Peer-reviewed

    NAKASAKO Masayoshi, MAKI-YONEKURA Saori, HIKIMA Takaaki, TAKAHASHI Yukio, SUZUKI Akihiro, MATSUNAGA Sachihiro, INUI Yayoi, TONO Kensuke, KAMESHIMA Takashi, JOTI Yasumasa, INUBUSHI Yuichi, OROGUCHI Tomotaka, HOSHI Takahiko, SEKIGUCHI Yuki, KOBAYASHI Amane, HASHIMOTO Saki, SHIRAHAMA Keiya, YAMAMOTO Masaki, TAKAYAMA Yuki, YONEKURA Koji

    Nihon Kessho Gakkaishi　56　(1)　27-35　2014

    Publisher: The Crystallographic Society of Japan

    DOI： 10.5940/jcrsj.56.27 　

    ISSN： 0369-4585

    More details Close

    Coherent X-ray diffraction imaging（CXDI）is a lens-less imaging technique that can visualize the structures of non-crystalline particles with micro- to sub-micrometer dimensions. In CXDI experiments, spatially isolated particles are irradiated by X-ray beam with high transverse coherence. Projection images of the particles along the incident X-ray direction are then directly reconstructed from the diffraction amplitude using a phase retrieval algorithm. Here we report CXDI experiments carried out at the X-ray free electron laser（XFEL）facility SACLA, and describe the theoretical background of CXDI, development of a diffractometer and data processing.

  46. Light-induced Conformational Changes of LOV1 (Light Oxygen Voltage-sensing Domain 1) and LOV2 Relative to the Kinase Domain and Regulation of Kinase Activity in Chlamydomonas Phototropin Peer-reviewed

    Koji Okajima, Yusuke Aihara, Yuki Takayama, Mihoko Nakajima, Sachiko Kashojiya, Takaaki Hikima, Tomotaka Oroguchi, Amane Kobayashi, Yuki Sekiguchi, Masaki Yamamoto, Tomomi Suzuki, Akira Nagatani, Masayoshi Nakasako, Satoru Tokutomi

    JOURNAL OF BIOLOGICAL CHEMISTRY　289　(1)　413-422　2014/01

    DOI： 10.1074/jbc.M113.515403 　

    ISSN： 0021-9258

    eISSN： 1083-351X

  47. Coherent Diffraction Imaging Analysis of Shape-Controlled Nanoparticles with Focused Hard X-ray Free-Electron Laser Pulses Peer-reviewed

    Yukio Takahashi, Akihiro Suzuki, Nobuyuki Zettsu, Tomotaka Oroguchi, Yuki Takayama, Yuki Sekiguchi, Amane Kobayashi, Masaki Yamamoto, Masayoshi Nakasako

    NANO LETTERS　13　(12)　6028-6032　2013/12

    DOI： 10.1021/nl403247x 　

    ISSN： 1530-6984

    eISSN： 1530-6992

  48. KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging Peer-reviewed

    Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Keiya Shirahama, Masaki Yamamoto, Takaaki Hikima, Koji Yonekura, Saori Maki-Yonekura, Yoshiki Kohmura, Yuichi Inubushi, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Inui, Kensuke Tono, Takashi Kameshima, Yasumasa Joti, Takahiko Hoshi

    REVIEW OF SCIENTIFIC INSTRUMENTS　84　(9)　093705　2013/09

    DOI： 10.1063/1.4822123 　

    ISSN： 0034-6748

    eISSN： 1089-7623

  49. 蛋白質水和構造の実験研究 Peer-reviewed

    中迫雅由, 苙口友隆, 高山裕貴, 中島真, 松井夕花

    アンサンブル　15　(1)　7-18　2013/01

    Publisher: 分子シミュレーション研究会

    DOI： 10.11436/mssj.15.7 　

    ISSN： 1884-6750

  50. Development and prospect of theory in coherent X-ray diffraction imaging Peer-reviewed

    NAKASAKO Masayoshi, OROGUCHI Tomotaka, TAKAYAMA Yuki, KOBAYASHI Amane, KODAMA Wataru, SAKAMOTO Keita, YOSHIDOME Takashi, IKEGUCHI Mitsunori

    放射光　26　(1)　11-25　2013/01

    Publisher: 日本放射光学会

    ISSN： 0914-9287

  51. Coherent X-Ray Diffraction Imaging of Non-Crystalline Particles Peer-reviewed

    NAKASAKO Masayoshi, TAKAYAMA Yuki, OROGUCHI Tomotaka, SHIRAHAMA Keiya, SEKIGUCHI Yuki, YAMAMOTO Masaki, YONEKURA Koji, HIKIMA Takaaki, MAKI YONEKURA Saori, TAKAHASHI Yukio, SUZUKI Akihiro, MATSUNAGA Sachihiro, KATO Shoichi, HOSHI Takahiko

    レーザー研究　40　(9)　680-686　2012/11

    ISSN： 0387-0200

  52. Coherent X-ray Diffraction Imaging of non-Crystalline Particles Using X-ray Free Electron Laser Peer-reviewed

    中迫雅由, 高山裕貴, 苙口友隆, 白濱圭也, 関口優希, 山本雅貴, 米倉功治, 引間孝明, 眞木‐さおり, 高橋幸生, 鈴木明大, 松永幸大, 加藤翔一, 星貴彦

    オプトロニクス　368　(8)　101-106　2012/08

    Publisher: オプトロニクス社

    ISSN： 0286-9659

  53. Humidity-controlled preparation of frozen-hydrated biological samples for cryogenic coherent x-ray diffraction microscopy Peer-reviewed

    Yuki Takayama, Masayoshi Nakasako

    REVIEW OF SCIENTIFIC INSTRUMENTS　83　(5)　054301　2012/05

    DOI： 10.1063/1.4718359 　

    ISSN： 0034-6748

  54. A few low-frequency normal modes predominantly contribute to conformational responses of hen egg white lysozyme in the tetragonal crystal to variations of molecular packing controlled by environmental humidity Peer-reviewed

    Yuki Takayama, Masayoshi Nakasako

    BIOPHYSICAL CHEMISTRY　159　(2-3)　237-246　2011/12

    DOI： 10.1016/j.bpc.2011.07.001 　

    ISSN： 0301-4622

    eISSN： 1873-4200

  55. Light-Induced Movement of the LOV2 Domain in an Asp720Asn Mutant LOV2-Kinase Fragment of Arabidopsis Phototropin 2 Peer-reviewed

    Yuki Takayama, Masayoshi Nakasako, Koji Okajima, Aya Iwata, Sachiko Kashojiya, Yuka Matsui, Satoru Tokutomi

    BIOCHEMISTRY　50　(7)　1174-1183　2011/02

    DOI： 10.1021/bi101689b 　

    ISSN： 0006-2960

  56. Redox-Dependent Domain Rearrangement of Protein Disulfide Isomerase from a Thermophilic Fungus Peer-reviewed

    Masayoshi Nakasako, Aya Maeno, Eiji Kurimoto, Takushi Harada, Yoshiki Yamaguchi, Toshihiko Oka, Yuki Takayama, Aya Iwata, Koichi Kato

    BIOCHEMISTRY　49　(32)　6953-6962　2010/08

    DOI： 10.1021/bi1006089 　

    ISSN： 0006-2960

Show all ︎Show first 5

Misc. 8

  1. 次世代放射光施設「NanoTerasu（ナノテラス）」のバイオサイエンスでの活用 Invited

    高山裕貴, 原田昌彦, 村松淳司, 日髙將文

    バイオサイエンスとインダストリー　83　(1)　2025/01

  2. BL24XU (Hyogo ID)

    Y. Urushihara, Y. Takayama, S. Kuwamoto, L. Cong, M. Yoshimura, Y. Tsusaka, Y. Kagoshima, S. Suzuki, K. Yokoyama, J. Matsui

    SPring-8 / SACLA annual report　FY2021　145-146　2023/07

  3. 食・農領域での次世代放射光利活用の推進 Invited

    原田昌彦, 高山裕貴, 日髙將文

    MICROOPTICS NEWS　41　23-28　2023

  4. Dynamic nanoimaging of extended objects via hard X-ray multiple-shot coherent diffraction with projection illumination optics Invited

    Yuki Takayama, Yasushi Kagoshima

    SPring-8/SACLA Research Frontiers 2021　56-57　2022

  5. コヒーレントX線を用いたレンズレス時空間階層イメージング法の開発 Invited

    高山裕貴, 篭島靖

    電気学会 光・量子デバイス研究会 研究会論文　OQD21068　2021/12

  6. コヒーレントX線を用いたレンズレス時空間階層イメージング法の開発（SPRUC 2021 Young Scientist Award受賞 研究報告） Invited Peer-reviewed

    高山裕貴

    SPring-8 / SACLA 利用者情報　26　349-355　2021

  7. BL24XU (Hyogo ID)

    Yuki Takayama, Yoshiyuki Tsusaka, Yasushi Kagoshima, Shigeo Kuwamoto, Yoshimasa Urushihara, Lei Li, Souichi Nose, Taiki Sudo, Masashi Yoshimura, Kazushi Yokoyama, Junji Matsui

    SPring-8・SACLA Annual Report　FY2018　132-135　2020/07

  8. Signal enhancement and Patterson-search phasing for higher-spatial-resolution coherent X-ray diffraction imaging of biological objects Invited Peer-reviewed

    Takayama Y, Yonekura K

    SPring-8/SACLA Research Frontiers 2015　132-133　2016

Show all ︎Show first 5

Books and Other Publications 1

  1. 'Coherent X-ray diffraction imaging of Cyanidioschyzon merolae' in Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology

    Sekiguchi Y, Kobayashi A, Takayama Y, Oide M, Fukuda A, Yamamoto T, Okajima K, Oroguchi T, Hirakawa T, Inui Y, Matsunaga S, Yamamoto M, Nakasako M

    Springer　2018/02

    ISBN: 9789811061004

Presentations 28

  1. Hard X-ray Ptychography and Multi-Shot Coherent Diffraction Imaging with Coherent Projection Illumination Optics

    Yuki Takayama, Keizo Fukuda, Motoki Kawashima, Yuki Aoi, Shotaro Oka, Hiroshi Ohno, Tatsuki Akada, Takumi Ikeda, Yasushi Kagoshima

    Materials Research Meeting 2019　2019/12/13

  2. Hard X-ray ptychography with coherent projection illumination optics Invited

    Yuki Takayama, Keizo Fukuda, Motoki Kawashima, Yuki Aoi, Tatsuki Akada, Takumi Ikeda, Yasushi Kagoshima

    The 15th Symposium of Japanese Research Community on X-ray Imaging Optics　2019/10/26

  3. Practical Designing of Inverse-Phase Composite Zone Plate for Deeper Depth of Focus

    Yasushi Kagoshima, Yuki Takayama

    The 15th Symposium of Japanese Research Community on X-ray Imaging Optics　2019/10/25

  4. Measurement of horizontal beam emittance of undulator radiation by tandem-double-slit optical system

    Yasushi Kagoshima, Tatsuki Akada, Takumi Ikeda, Motoki Kawashima, Yuki Aoi, Yuki Takayama

    The 15th Symposium of Japanese Research Community on X-ray Imaging Optics　2019/10/25

  5. X-ray lensless nano-imaging toward investigation of nanostructured materials and devices

    Yuki Takayama, Yasushi Kagoshima, Shotaro Oka, Hiroshi Ohno

    Symposium on bioinspired design of advanced materials　2019/10/10

  6. The physical properties and structure of wheat noodles (Tenobe Somen) after different storage periods Invited

    Natsuki Hosoda, Yuki Takayama, Tatsuki Akada, Yuki Aoi, Nobutake Hara, Miki Yoshimura

    The China-Japan Joint Symposium on Food Hydrocolloids　2019/09/26

  7. Proposal for Inverse-Phase Composite Zone Plate for Deeper Depth of Focus International-presentation

    Yasushi Kagoshima, Yuki Takayama

    14th International Conference on X-ray Microscopy　2018/08/22

  8. Cryogenic coherent X-ray diffraction imaging techniques for structural analyses of biological cells and cellular organelles International-presentation

    Amane Kobayashi, Yuki Takayama, Tomotaka Oroguchi, Koji Okajima, Mao Oide, Masaki Yamamoto, Masayoshi Nakasako

    14th International Conference on X-ray Microscopy　2018/08/19

  9. Coherent X-ray Diffraction Imaging at SPring-8 Hyogo Beamline BL24XU International-presentation

    Yuki Takayama, Yuki Takami, Keizo Fukuda, Takamasa Miyagawa, Yoshimasa Urushihara, Shigeo Kuwamoto, Yasushi Kagoshima

    The 24th Congress of the International Commission for Optics　2017/08/22

  10. Preparation of Lanthanum Silicate Electrolyte with High Conductivity and High Chemical Stability International-presentation

    Hikaru Hayakawa, Atsushi Mineshige, Takuma Nishimoto, Akie Heguri, Tetsuo Yazawa, Yuki Takayama, Yasushi Kagoshima, Hidekazu Takano, Shingo Takeda, Junji Matsui

    21st International Conference on Solid State Ionics　2017/06/18

  11. Coherent X-ray Diffraction Imaging at SPring-8 Hyogo Beamline BL24XU International-presentation

    Yuki Takayama, Yuki Takami, Takamasa Miyagawa, Yasushi Kagoshima

    International Conference on X-ray Optics and Application 2017　2017/04/18

  12. Blue-light induced structural changes of phototropin revealed by small-angle X-ray scattering International-presentation

    Mao Oide, Koji Okajima, Yuki Sekiguchi, Yuki Takayama, Tomotaka Oroguchi, Sachiko Kashojiya, Takaaki Hikima, Masaki Yamamoto, Masayoshi Nakasako

    Okazaki Conference 2017 “Grand Challenges in Small-angle Scattering”　2017/03/18

  13. Cryogenic coherent diffraction imaging of biological samples with synchrotron and X-ray laser International-presentation

    Yuki Takayama, Masaki Yamamoto, Koji Yonekura, Yuki Sekiguchi, Amane Kobayashi, Koji Okajima, Tomotaka Oroguchi, Masayoshi Nakasako, Yayoi Inui, Sachihiro Matsunaga

    13th International Conference on X-ray Microscopy　2016/08/18

  14. Cryogenic coherent X-ray diffraction imaging of organelles at SACLA International-presentation

    Yuki Takayama, Koji Yonekura, Masaki Yamamoto, Amane Kobayashi, Yuki Sekiguchi, Tomotaka Oroguchi, Masayoshi Nakasako, Yayoi Inui, Sachihiro Matsunaga

    The 1st SACLA Workshop on Femtosecond Crystallography　2015/03/26

  15. Coherent X-ray diffraction imaging of chloroplasts from Cyanidioschyzon merolae by using X-ray free electron laser International-presentation

    Yuki Sekiguchi, Amane Kobayashi, Tomotaka Oroguchi, Masayoshi Nakasako, Inui Yayoi, Sachihiro Matsunaga, Yuki Takayama, Masaki Yamamoto

    The 2nd International Symposium on Plant Environmental Sensing　2015/03/14

  16. Cryogenic coherent X-ray diffraction imaging of biological particles at SACLA International-presentation

    Yuki Takayama, Masayoshi Nakasako, Tomotaka Oroguchi, Yuki Sekiguchi, Masaki Yamamoto, Koji Yonekura, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Tsujimoto-Inui

    23rd Congress and General Assembly of the International Union of Crystallography　2014/08/07

  17. Single-shot 3D structure determination of nanocrystals with femtosecond X-ray free electron laser pulses International-presentation

    Jianwei Miao, Rui Xu, Huaidong Jiang, Changyong Song, Jose A. Rodriguez, Zhifeng Huang, Chien-Chun Chen, Daewoong Nam, Jaehyun Park, Marcus Gallagher-Jones, Sangsoo Kim, Sunam Kim, Akihiro Suzuki, Yuki Takayama, Tomotaka Oroguchi, Yukio Takahashi, Jiadong Fan, Yunfei Zou, Takaki Hatsui, Yuichi Inubushi, Takashi Kameshima, Koji Yonekura, Kensuke Tono, Tadashi Togashi, Takahiro Sato, Masaki Yamamoto, Masayoshi Nakasako, Makina Yabashi, Tetsuya Ishikawa

    The 6-th International Workshop on FEL Science: New Horizon of XFEL Science　2013/11/04

  18. Cryogenic CXDI experiments for non-crystalline particles using KOTOBUKI-1 apparatus at SACLA: the present status and future plan International-presentation

    Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Keiya Shirahama, Masaki Yamamoto, Takaaki Hikima, Koji Yonekura, Saori Maki-Yonekura, Yoshiki Kohmura, Yuichi Inubushi, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Inui, Yuichi Ichikawa, Hitoshi Kurumizaka, Kensuke Tono, Takashi Kameshima, Yasumasa Joti, Takahiko Hoshi

    The 6-th International Workshop on FEL Science: New Horizon of XFEL Science　2013/11/04

  19. Image reconstruction from diffraction patterns in coherent X-ray diffraction imaging using the dark-field phase-retrieval method International-presentation

    Amane Kobayashi, Yuki Takayama, Tomotaka Oroguchi, Masayoshi Nakasako

    The 7th Asia Oceania Forum for Synchrotron Radiation Research　2013/09/22

  20. KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging at SPring-8 and SACLA International-presentation

    Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Keiya Shirahama, Masaki Yamamoto, Takaaki Hikima, Koji Yonekura, Saori Maki-Yonekura, Yoshiki Kohmura, Yuichi Inubushi, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Tsujimoto-Inui, Kensuke Tono, Takashi Kameshima, Yasumasa Joti, Takahiko Hoshi

    The 7th Asia Oceania Forum for Synchrotron Radiation Research　2013/09/22

  21. Software suite “SHITENNO” for automatically processing diffraction patterns in coherent X-ray diffraction imaging experiments at SACLA International-presentation

    Yuki Sekiguchi, Tomotaka Oroguchi, Yuki Takayama, Masayoshi Nakasako

    The 7th Asia Oceania Forum for Synchrotron Radiation Research　2013/09/22

  22. Humidity-controlled preparation of frozen-hydrated biological samples for cryogenic coherent X-ray diffraction imaging International-presentation

    Yuki Takayama, Masayoshi Nakasako, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Masaki Yamamoto, Koji Yonekura, Takaaki Hikima, Saori Maki-Yonekura, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Tsujimoto-Inui, Shoichi Kato, Takahiko Hoshi

    11th International Conference on Biology and Synchrotron Radiation　2013/09/11

  23. Cryogenic CXDI of non-crystalline particles at SACLA International-presentation

    Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Makoto Nakajima, Masaki Yamamoto, Koji Yonekura, Takaaki Hikima, Saori Maki-Yonekura, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Shoichi Kato, Takahiko Hoshi

    4-th Japan-France Joint Seminar　2013/01/09

  24. Cryogenic CXDI of non-crystalline particles at SACLA International-presentation

    Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Masaki Yamamoto, Koji Yonekura, Takaaki Hikima, Saori Maki-Yonekura, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Shoichi Kato, Takahiko Hoshi

    Coherence 2012 International Workshop on Phase Retrieval and Coherent Scattering　2012/01/20

  25. Humidity-controlled preparation of frozen-hydrated, non-crystalline particles for cryogenic coherent X-ray diffraction imaging International-presentation

    Yuki Takayama, Masayoshi Nakasako, Tomotaka Oroguchi, Yuki Sekiguchi, Masaki Yamamoto, Koji Yonekura, Takaaki Hikima, Saori Maki-Yonekura, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Shoichi Kato, Takahiko Hoshi

    Coherence 2012 International Workshop on Phase Retrieval and Coherent Scattering　2012/01/18

  26. Coherent X-ray diffraction microscopy of non-crystalline biological particles at cryogenic temperatures International-presentation

    Masayoshi Nakasako, Masaki Yamamoto, Yuki Takayama, Wataru Kodama, Keita Sakamoto, Yoshinori Nishino, Koji Yonekura, Kazuhiro Maeshima, Keiya Shirahama, Keiichi Namba

    The 3rd Workshop on FEL Science: “Emerging X-ray Applications in Biological Systems-II”　2010/10/05

  27. Protein crystallography for humidity-controlled crystals International-presentation

    Yuki Takayama, Masayoshi Nakasako

    International Symposium on “Molecular Science for Supra Functional Systems”　2009/06/05

  28. Structural and informatics studies on plant blue-light receptor phototropin 1 and 2 International-presentation

    Masayoshi Nakasako, Yuki Takayama, Aya Iwata, Kazunori Zikihara, Kohji Okajima, Daisuke Matsuoka, Hitomi Katsura, Satoru Tokutomi

    International Symposium on “Molecular Science for Supra Functional Systems”　2009/06/05

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Research Projects 10

  1. Development of a local structure analysis method for complex oxides composed of light elements using a synchrotron soft X-ray light source

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2024/04/01 - 2029/03/31

  2. 可視化技術を活用した野菜における生理障害発生機構の解明

    金山 喜則, 高山 裕貴, 栗原 大輔, 加藤 一幾, 西山 学

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2025/04/01 - 2028/03/31

  3. 放射線損傷限界を超える細胞の分子分解能三次元イメージング Competitive

    高山 裕貴

    Offer Organization: 日本学術振興会

    System: 科学研究費 若手研究

    2019/04 - 2022/03

  4. 有機栽培ケールの構造・栄養学的観点による相関解析に基づいた機能性野菜チップスの開発 Competitive

    鯛 かおる

    Offer Organization: 兵庫県

    System: 「農」イノベーションひょうご研究開発プロジェクト支援事業「実用化研究枠」

    2018/07 - 2019/03

  5. 栄養学的・構造学的評価による高たんぱく・高栄養の新規スマイルケア食品の開発 Competitive

    鯛 かおる

    Offer Organization: 兵庫県

    System: 兵庫県最先端技術研究事業(COEプログラム)「可能性調査・研究」

    2018/07 - 2019/03

  6. 材料・細胞試料の階層構造可視化に向けた 広 視野・高分解能 X 線顕微鏡の開発 Competitive

    高山 裕貴

    Offer Organization: ひょうご科学技術協会

    System: 学術研究助成

    2017/04 - 2018/03

  7. ホログラフィックX線回折イメージング法による単細胞藻類細胞分裂機構の研究 Competitive

    高山 裕貴

    Offer Organization: 日本学術振興会

    System: 科学研究費 若手研究(B)

    2016/04 - 2018/03

  8. コヒーレントX線回折イメージング法とX線結像光学系の融合による高分解能・高精度X線顕微鏡の開発 Competitive

    高山 裕貴

    Offer Organization: 兵庫県立大学

    System: 特別研究助成(先導的プロジェクト)

    2016/07 - 2017/03

  9. コヒーレントX線回折顕微鏡法による、単細胞真核生物シゾン細胞分裂過程のナノメートル分解能空間階層イメージング Competitive

    高山 裕貴

    Offer Organization: 理化学研究所

    System: 基礎科学特別研究員研究費

    2013/04 - 2016/03

  10. コヒーレントX線回折顕微鏡法による真核細胞のナノメートル分解能空間階層構造解析 Competitive

    高山 裕貴

    Offer Organization: 日本学術振興会

    System: 科学研究費 研究活動スタート支援

    2013/08 - 2015/03

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Teaching Experience 3

  1. 装置実習II (大学院・ビームライン実習)　兵庫県立大学 大学院 理学研究科

  2. 英語科学問題演習(2年次)　兵庫県立大学理学部

  3. 科学英語(4年次)　兵庫県立大学理学部