研究者詳細

顔写真

ハーリド　ハタビー
Khaled Hatabi
Khaled Hatabi
所属
大学院医学系研究科　医科学専攻　細胞生物学講座（放射線生物学分野）
職名
助教
学位

  • 博士（医学）（東北大学）

経歴 2

  • 2022年11月 ～ 継続中
    東北大学　大学院医学系研究科　助教

  • 2022年10月 ～ 2022年11月
    東北大学　大学院医学系研究科　助手

学歴 2

  • 東北大学　大学院医学系研究科　医科学専攻

    2018年10月 ～ 2022年9月

  • アレッポ大学　医学部

    2011年9月 ～ 2018年2月

所属学協会 1

  • 日本再生医療学会

研究キーワード 5

  • Muse細胞

  • 間葉系幹細胞

  • 培養細胞

  • 細胞生物学

  • 分子生物学

研究分野 1

  • ライフサイエンス / 細胞生物学 /

受賞 2

  1. 令和2年度ブースター研究奨励賞 優秀賞

    2020年2月　東北大学

  2. 日本政府（文部科学省）奨学金

    2018年4月

論文 3

  1. Oxamate, an LDHA Inhibitor, Inhibits Stemness, Including EMT and High DNA Repair Ability, Induces Senescence, and Exhibits Radiosensitizing Effects in Glioblastoma Cells 査読有り

    International Journal of Molecular Sciences　26　(12)　5710　2025年6月10日

    DOI： 10.3390/ijms26125710 　

  2. FDX1 Regulates the Phosphorylation of ATM, DNA-PKcs Akt, and EGFR and Affects Radioresistance Under Severe Hypoxia in the Glioblastoma Cell Line T98G 査読有り

    Takuma Hashimoto, Kazuki Tsubota, Khaled Hatabi, Yoshio Hosoi

    International Journal of Molecular Sciences　2025年4月4日

    DOI： 10.3390/ijms26073378 　

  3. Inhibition of Gap Junctional Intercellular Communication Upregulates Pluripotency Gene Expression in Endogenous Pluripotent Muse Cells 査読有り

    Khaled Hatabi, Yukari Hirohara, Yoshihiro Kushida, Yasumasa Kuroda, Shohei Wakao, James Trosko, Mari Dezawa

    Cells　11　(17)　2701-2701　2022年8月30日

    出版者・発行元： MDPI AG

    DOI： 10.3390/cells11172701 　

    eISSN：2073-4409

    詳細を見る 詳細を閉じる

    Gap junctions (GJ) are suggested to support stem cell differentiation. The Muse cells that are applied in clinical trials are non-tumorigenic pluripotent-like endogenous stem cells, can be collected as stage-specific embryonic antigen 3 (SSEA-3+) positive cells from multiple tissues, and show triploblastic differentiation and self-renewability at a single cell level. They were reported to up-regulate pluripotency gene expression in suspension. We examined how GJ inhibition affected pluripotency gene expression in adherent cultured-Muse cells. Muse cells, mainly expressing gap junction alpha-1 protein (GJA1), reduced GJ intercellular communication from ~85% to 5–8% after 24 h incubation with 120 μM 18α-glycyrrhetinic acid, 400 nM 12-O-tetradecanoylphorbol-13-acetate, and 90 μM dichlorodiphenyltrichloroethane, as confirmed by a dye-transfer assay. Following inhibition, NANOG, OCT3/4, and SOX2 were up-regulated 2–4.5 times more; other pluripotency-related genes, such as KLF4, CBX7, and SPRY2 were elevated; lineage-specific differentiation-related genes were down-regulated in quantitative-PCR and RNA-sequencing. Connexin43-siRNA introduction also confirmed the up-regulation of NANOG, OCT3/4, and SOX2. YAP, a co-transcriptional factor in the Hippo signaling pathway that regulates pluripotency gene expression, co-localized with GJA1 (also known as Cx43) in the cell membrane and was translocated to the nucleus after GJ inhibition. Adherent culture is usually more suitable for the stable expansion of cells than is a suspension culture. GJ inhibition is suggested to be a simple method to up-regulate pluripotency in an adherent culture that involves a Cx43-YAP axis in pluripotent stem cells, such as Muse cells.

MISC 1

  1. 重度な低酸素環境下における癌細胞の放射線抵抗性とAMPKα/Sp1/ATMの寄与 査読有り

    橋本 拓磨, ハーリド ハタビー, 細井 義夫

    54　(7)　42-44　2022年5月