Details of the Researcher

PHOTO

Khaled Hatabi
Section
Graduate School of Medicine
Job title
Assistant Professor
Degree

  • 博士（医学）（東北大学）

Research History 2

  • 2022/11 - Present
    Tohoku University　Graduate School of Medicine　Assistant Professor

  • 2022/10 - 2022/11
    Tohoku University　Graduate School of Medicine　Assistant

Education 2

  • Tohoku University　Graduate School of Medicine　Department of Medical Sciences

    2018/10 - 2022/09

  • Aleppo University　Faculty of Medicine

    2011/09 - 2018/02

Professional Memberships 1

  • The Japanese Society for Regenerative Medicine

Research Interests 5

  • Muse細胞

  • 間葉系幹細胞

  • 培養細胞

  • 細胞生物学

  • 分子生物学

Research Areas 1

  • Life sciences / Cell biology /

Awards 2

  1. Booster Research Incentive Award

    2020/02　Tohoku University

  2. Japanese Government （MEXT） Scholarship

    2018/04

Papers 3

  1. Oxamate, an LDHA Inhibitor, Inhibits Stemness, Including EMT and High DNA Repair Ability, Induces Senescence, and Exhibits Radiosensitizing Effects in Glioblastoma Cells Peer-reviewed

    Takuma Hashimoto, Go Ushikubo, Naoya Arao, Khaled Hatabi, Kazuki Tsubota, Yoshio Hosoi

    International Journal of Molecular Sciences　26　(12)　5710　2025/06/10

    DOI： 10.3390/ijms26125710 　

  2. FDX1 Regulates the Phosphorylation of ATM, DNA-PKcs Akt, and EGFR and Affects Radioresistance Under Severe Hypoxia in the Glioblastoma Cell Line T98G Peer-reviewed

    Takuma Hashimoto, Kazuki Tsubota, Khaled Hatabi, Yoshio Hosoi

    International Journal of Molecular Sciences　2025/04/04

    DOI： 10.3390/ijms26073378 　

  3. Inhibition of Gap Junctional Intercellular Communication Upregulates Pluripotency Gene Expression in Endogenous Pluripotent Muse Cells Peer-reviewed

    Khaled Hatabi, Yukari Hirohara, Yoshihiro Kushida, Yasumasa Kuroda, Shohei Wakao, James Trosko, Mari Dezawa

    Cells　11　(17)　2701-2701　2022/08/30

    Publisher: MDPI AG

    DOI： 10.3390/cells11172701 　

    eISSN： 2073-4409

    More details Close

    Gap junctions (GJ) are suggested to support stem cell differentiation. The Muse cells that are applied in clinical trials are non-tumorigenic pluripotent-like endogenous stem cells, can be collected as stage-specific embryonic antigen 3 (SSEA-3+) positive cells from multiple tissues, and show triploblastic differentiation and self-renewability at a single cell level. They were reported to up-regulate pluripotency gene expression in suspension. We examined how GJ inhibition affected pluripotency gene expression in adherent cultured-Muse cells. Muse cells, mainly expressing gap junction alpha-1 protein (GJA1), reduced GJ intercellular communication from ~85% to 5–8% after 24 h incubation with 120 μM 18α-glycyrrhetinic acid, 400 nM 12-O-tetradecanoylphorbol-13-acetate, and 90 μM dichlorodiphenyltrichloroethane, as confirmed by a dye-transfer assay. Following inhibition, NANOG, OCT3/4, and SOX2 were up-regulated 2–4.5 times more; other pluripotency-related genes, such as KLF4, CBX7, and SPRY2 were elevated; lineage-specific differentiation-related genes were down-regulated in quantitative-PCR and RNA-sequencing. Connexin43-siRNA introduction also confirmed the up-regulation of NANOG, OCT3/4, and SOX2. YAP, a co-transcriptional factor in the Hippo signaling pathway that regulates pluripotency gene expression, co-localized with GJA1 (also known as Cx43) in the cell membrane and was translocated to the nucleus after GJ inhibition. Adherent culture is usually more suitable for the stable expansion of cells than is a suspension culture. GJ inhibition is suggested to be a simple method to up-regulate pluripotency in an adherent culture that involves a Cx43-YAP axis in pluripotent stem cells, such as Muse cells.

Misc. 1

  1. 重度な低酸素環境下における癌細胞の放射線抵抗性とAMPKα/Sp1/ATMの寄与 Peer-reviewed

    橋本 拓磨, ハーリド ハタビー, 細井 義夫

    54　(7)　42-44　2022/05