Details of the Researcher

PHOTO

Hideki Takahashi
Section
Graduate School of Agricultural Science
Job title
Professor
Degree

  • 博士（農学）（東北大学）

Committee Memberships 24

  • 日本植物病理学会東北部会　部会長

    2014/04 - Present

  • 北日本病害虫研究会　評議員

    2014/04 - Present

  • 日本植物病理学会東北部会　部会長

    2014/04 - Present

  • 北日本病害虫研究会　評議員

    2014/04 - Present

  • 日本植物病理学会評議員会　日本植物病理学会評議員

    2010/04 - Present

  • 国際植物病理学会 (ISPP)　Council Member

    2010/04 - Present

  • 日本植物病理学会評議員会　日本植物病理学会評議員

    2010/04 - Present

  • 国際植物病理学会 (ISPP)　Council Member

    2010/04 - Present

  • 日本植物病理学会 JABEE対応委員会　JABEE対応委員

    2008/04 - Present

  • 日本植物病理学会 JABEE対応委員会　JABEE対応委員

    2008/04 - Present

  • 日本植物病理学会東北部会　幹事(1996-)

    1999/04 - Present

  • 日本植物病理学会東北部会　幹事(1996-)

    1999/04 - Present

  • 日本植物病理学会学会賞選考委員　賞選考委員

    2013/04 - 2015/03

  • 日本植物病理学会学会賞選考委員　賞選考委員

    2013/04 - 2015/03

  • Journal of General Plant Pathology　Associate Editor

    2010/04 - 2013/03

  • 日本植物病理学会報編集委員会　原著論文編集委員

    2010/04 - 2013/03

  • Journal of General Plant Pathology　Associate Editor

    2010/04 - 2013/03

  • 日本植物病理学会報編集委員会　原著論文編集委員

    2010/04 - 2013/03

  • Plant Cell Physiology編集委員会　Editorial Board

    2009/04 - 2011/03

  • Plant Cell Physiology編集委員会　Editorial Board

    2009/04 - 2011/03

  • 日本農芸科学会「化学と生物」企画委員会　企画委員

    2001/04 - 2005/03

  • 日本農芸科学会「化学と生物」企画委員会　企画委員

    2001/04 - 2005/03

  • 日本植物病理学会　幹事(1996-)

    1996/04 - 1999/03

  • 日本植物病理学会　幹事(1996-)

    1996/04 - 1999/03

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Professional Memberships 3

  • 日本農芸化学会

  • 日本植物生理学会

  • 日本植物病理学会

Research Interests 9

  • 病原性

  • 宿主特異性

  • 病徴発現

  • 生物農薬

  • 誘導抵抗性

  • シグナル伝達

  • 非病原性遺伝子

  • 抵抗性遺伝子

  • 病害抵抗性

Research Areas 1

  • Environmental science/Agricultural science / Conservation science (plants) / Plant Pathology

Awards 3

  1. 日本植物病理学会賞

    2008/04　日本植物病理学会

  2. 日本植物病理学会論文賞

    2007/03/28　日本植物病理学会

  3. 学術奨励賞(日本植物病理学会)

    1994/04　日本植物病理学会　植物ウイルス感染における病徴発現機構に関する研究

Papers 137

  1. A single amino acid at position 31 in the N-terminus of the coat protein of cucumber mosaic virus determines its avirulence function for RCY1-conferred virus resistance

    Hideki Takahashi, Yuki Ando, Yoshinori Kanayama, Shuhei Miyashita

    Archives of Virology　169　(3)　2024/03

    DOI： 10.1007/s00705-024-05961-4 　

    ISSN： 0304-8608

    eISSN： 1432-8798

  2. High ultraviolet-B sensitivity due to lower CPD photolyase activity is needed for biotic stress response to the rice blast fungus, Magnaporthe oryzae

    Gideon S. Mmbando, Sugihiro Ando, Hideki Takahashi, Jun Hidema

    Photochemical and Photobiological Sciences　2023

    DOI： 10.1007/s43630-023-00379-4 　

    ISSN： 1474-905X

    eISSN： 1474-9092

  3. Isolation of Burkholderia jumbo phages and their utilization as biocontrol agents to suppress rice seedling rot disease

    Ayane Kanaizuka, Ryota Sasaki, Shuhei Miyashita, Sugihiro Ando, Kumiko Ito, Toshiyuki Fukuhara, Hideki Takahashi

    JOURNAL OF GENERAL PLANT PATHOLOGY　89　(1)　24-34　2023/01

    DOI： 10.1007/s10327-022-01107-z 　

    ISSN： 1345-2630

    eISSN： 1610-739X

  4. Activation of plant immunity by exposure to dinitrogen pentoxide gas generated from air using plasma technology

    Daiki Tsukidate, Keisuke Takashima, Shota Sasaki, Shuhei Miyashita, Toshiro Kaneko, Hideki Takahashi, Sugihiro Ando

    PLOS ONE　17　(6)　e0269863-e0269863　2022/06/24

    Publisher: Public Library of Science (PLoS)

    DOI： 10.1371/journal.pone.0269863 　

    eISSN： 1932-6203

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    Reactive nitrogen species (RNS) play an important role in plant immunity as signaling factors. We previously developed a plasma technology to partially convert air molecules into dinitrogen pentoxide (N2O5), an RNS whose physiological action is poorly understood. To reveal the function of N2O5 gas in plant immunity, Arabidopsis thaliana was exposed to plasma-generated N2O5 gas once (20 s) per day for 3 days, and inoculated with Botrytis cinerea, Pseudomonas syringae pv. tomato DC3000 (Pst), or cucumber mosaic virus strain yellow (CMV(Y)) at 24 h after the final N2O5 gas exposure. Lesion size with B. cinerea infection was significantly (P < 0.05) reduced by exposure to N2O5 gas. Propagation of CMV(Y) was suppressed in plants exposed to N2O5 gas compared with plants exposed to the air control. However, proliferation of Pst in the N2O5-gas-exposed plants was almost the same as in the air control plants. These results suggested that N2O5 gas exposure could control plant disease depending on the type of pathogen. Furthermore, changes in gene expression at 24 h after the final N2O5 gas exposure were analyzed by RNA-Seq. Based on the gene ontology analysis, jasmonic acid and ethylene signaling pathways were activated by exposure of Arabidopsis plants to N2O5 gas. A time course experiment with qRT-PCR revealed that the mRNA expression of the transcription factor genes, WRKY25, WRKY26, WRKY33, and genes for tryptophan metabolic enzymes, CYP71A12, CYP71A13, PEN2, and PAD3, was transiently induced by exposure to N2O5 gas once for 20 s peaking at 1–3 h post-exposure. However, the expression of PDF1.2 was enhanced beginning from 6 h after exposure and its high expression was maintained until 24–48 h later. Thus, enhanced tryptophan metabolism leading to the synthesis of antimicrobial substances such as camalexin and antimicrobial peptides might have contributed to the N2O5-gas-induced disease resistance.

  5. Suppression of rice seedling rot caused by Burkholderia glumae in nursery soils using culturable bacterial communities from organic farming systems Peer-reviewed

    Sugihiro Ando, Masami Kasahara, Naoto Mitomi, Tom A. Schermer, Erika Sato, Shigenobu Yoshida, Seiya Tsushima, Shuhei Miyashita, Hideki Takahashi

    Journal of Plant Pathology　2022/02/11

    DOI： 10.1007/s42161-022-01066-6 　

  6. Plant death caused by inefficient induction of antiviral R-gene-mediated resistance may function as a suicidal population resistance mechanism

    Derib A. Abebe, Sietske van Bentum, Machi Suzuki, Sugihiro Ando, Hideki Takahashi, Shuhei Miyashita

    Communications Biology　4　(1)　2021/12

    DOI： 10.1038/s42003-021-02482-7 　

    eISSN： 2399-3642

  7. Isolation and Characterization of a Novel Jumbo Phage from Leaf Litter Compost and Its Suppressive Effect on Rice Seedling Rot Diseases. International-journal

    Ryota Sasaki, Shuhei Miyashita, Sugihiro Ando, Kumiko Ito, Toshiyuki Fukuhara, Hideki Takahashi

    Viruses　13　(4)　2021/03/31

    DOI： 10.3390/v13040591 　

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    Jumbo phages have DNA genomes larger than 200 kbp in large virions composed of an icosahedral head, tail, and other adsorption structures, and they are known to be abundant biological substances in nature. In this study, phages in leaf litter compost were screened for their potential to suppress rice seedling rot disease caused by the bacterium Burkholderia glumae, and a novel phage was identified in a filtrate-enriched suspension of leaf litter compost. The phage particles consisted of a rigid tailed icosahedral head and contained a DNA genome of 227,105 bp. The phage could lyse five strains of B. glumae and six strains of Burkholderia plantarii. The phage was named jumbo Burkholderia phage FLC6. Proteomic tree analysis revealed that phage FLC6 belongs to the same clade as two jumbo Ralstonia phages, namely RSF1 and RSL2, which are members of the genus Chiangmaivirus (family: Myoviridae; order: Caudovirales). Interestingly, FLC6 could also lyse two strains of Ralstonia pseudosolanacearum, the causal agent of bacterial wilt, suggesting that FLC6 has a broad host range that may make it especially advantageous as a bio-control agent for several bacterial diseases in economically important crops. The novel jumbo phage FLC6 may enable leaf litter compost to suppress several bacterial diseases and may itself be useful for controlling plant diseases in crop cultivation.

  8. Isolation and Characterization of a Novel Jumbo Phage from Leaf Litter Compost and Its Suppressive Effect on Rice Seedling Rot Diseases

    Ryota Sasaki, Shuhei Miyashita, Sugihiro Ando, Kumiko Ito, Toshiyuki Fukuhara, Hideki Takahashi

    Viruses　13　(4)　591-591　2021/03/31

    Publisher: MDPI AG

    DOI： 10.3390/v13040591 　

    eISSN： 1999-4915

    More details Close

    Jumbo phages have DNA genomes larger than 200 kbp in large virions composed of an icosahedral head, tail, and other adsorption structures, and they are known to be abundant biological substances in nature. In this study, phages in leaf litter compost were screened for their potential to suppress rice seedling rot disease caused by the bacterium Burkholderia glumae, and a novel phage was identified in a filtrate-enriched suspension of leaf litter compost. The phage particles consisted of a rigid tailed icosahedral head and contained a DNA genome of 227,105 bp. The phage could lyse five strains of B. glumae and six strains of Burkholderia plantarii. The phage was named jumbo Burkholderia phage FLC6. Proteomic tree analysis revealed that phage FLC6 belongs to the same clade as two jumbo Ralstonia phages, namely RSF1 and RSL2, which are members of the genus Chiangmaivirus (family: Myoviridae; order: Caudovirales). Interestingly, FLC6 could also lyse two strains of Ralstonia pseudosolanacearum, the causal agent of bacterial wilt, suggesting that FLC6 has a broad host range that may make it especially advantageous as a bio-control agent for several bacterial diseases in economically important crops. The novel jumbo phage FLC6 may enable leaf litter compost to suppress several bacterial diseases and may itself be useful for controlling plant diseases in crop cultivation.

  9. In vivo emergence of beige-like fat in chickens as physiological adaptation to cold environments. International-journal

    Rina Sotome, Akira Hirasawa, Motoi Kikusato, Taku Amo, Kyohei Furukawa, Anna Kuriyagawa, Kouichi Watanabe, Anne Collin, Hitoshi Shirakawa, Ryota Hirakawa, Yuta Tanitaka, Hideki Takahashi, Guoyao Wu, Tomonori Nochi, Tsuyoshi Shimmura, Craig H Warden, Masaaki Toyomizu

    Amino acids　53　(3)　381-393　2021/03

    DOI： 10.1007/s00726-021-02953-5 　

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    While it has been hypothesized that brown adipocytes responsible for mammalian thermogenesis are absent in birds, the existence of beige fat has yet to be studied directly. The present study tests the hypothesis that beige fat emerges in birds as a mechanism of physiological adaptation to cold environments. Subcutaneous neck adipose tissue from cold-acclimated or triiodothyronine (T3)-treated chickens exhibited increases in the expression of avian uncoupling protein (avUCP, an ortholog of mammalian UCP2 and UCP3) gene and some known mammalian beige adipocyte-specific markers. Morphological characteristics of white adipose tissues of treated chickens showed increased numbers of both small and larger clusters of multilocular fat cells within the tissues. Increases in protein levels of avUCP and mitochondrial marker protein, voltage-dependent anion channel, and immunohistochemical analysis for subcutaneous neck fat revealed the presence of potentially thermogenic mitochondria-rich cells. This is the first evidence that the capacity for thermogenesis may be acquired by differentiating adipose tissue into beige-like fat for maintaining temperature homeostasis in the subcutaneous fat 'neck warmer' in chickens exposed to a cold environment.

  10. Frequent asymptomatic infection with tobacco ringspot virus on melon fruit. International-journal

    Midori Tabara, Yukihiro Nagashima, Kai He, Xiaoning Qian, Kevin M Crosby, John Jifon, G K Jayaprakasha, Bhimanagouda Patil, Hisashi Koiwa, Hideki Takahashi, Toshiyuki Fukuhara

    Virus research　293　198266-198266　2021/02

    DOI： 10.1016/j.virusres.2020.198266 　

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    Melon is one of the most popular fruits worldwide and has been bred into various cultivars. RNA-sequencing using healthy melon fruit was performed to determine differences in gene expression among cultivars. Unexpected RNA-seq results revealed that viruses asymptomatically infected fruits at a high frequency (16 of 21 fruits examined were infected) and that viral transcripts highly accumulated in comparison with host transcripts (15 %-75 % of total reads). Their nucleotide sequences and phylogenetic analyses indicated that more than 10 novel isolates of tobacco ringspot virus (TRSV) were found in melon fruits. Asymptomatic infection with TRSV on melon fruits was confirmed by both immunoblot and RT-PCR analyses. Numerous isolates of TRSV generated and maintained in melon fields, and this is likely due to their asymptomatic infections. This TRSV melon isolate infected Nicotiana benthamiana plants with stunting and yellowing symptoms. This is the first report of frequent and asymptomatic infection of TRSV in consumable melon fruits.

  11. Priming for enhanced ARGONAUTE2 activation accompanies induced resistance to cucumber mosaic virus in Arabidopsis thaliana. International-journal

    Sugihiro Ando, Michal Jaskiewicz, Sei Mochizuki, Saeko Koseki, Shuhei Miyashita, Hideki Takahashi, Uwe Conrath

    Molecular plant pathology　22　(1)　19-30　2021/01

    DOI： 10.1111/mpp.13005 　

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    Systemic acquired resistance (SAR) is a broad-spectrum disease resistance response that can be induced upon infection from pathogens or by chemical treatment, such as with benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH). SAR involves priming for more robust activation of defence genes upon pathogen attack. Whether priming for SAR would involve components of RNA silencing remained unknown. Here, we show that upon leaf infiltration of water, BTH-primed Arabidopsis thaliana plants accumulate higher amounts of mRNA of ARGONAUTE (AGO)2 and AGO3, key components of RNA silencing. The enhanced AGO2 expression is associated with prior-to-activation trimethylation of lysine 4 in histone H3 and acetylation of histone H3 in the AGO2 promoter and with induced resistance to the yellow strain of cucumber mosaic virus (CMV[Y]). The results suggest that priming A. thaliana for enhanced defence involves modification of histones in the AGO2 promoter that condition AGO2 for enhanced activation, associated with resistance to CMV(Y). Consistently, the fold-reduction in CMV(Y) coat protein accumulation by BTH pretreatment was lower in ago2 than in wild type, pointing to reduced capacity of ago2 to activate BTH-induced CMV(Y) resistance. A role of AGO2 in pathogen-induced SAR is suggested by the enhanced activation of AGO2 after infiltrating systemic leaves of plants expressing a localized hypersensitive response upon CMV(Y) infection. In addition, local inoculation of SAR-inducing Pseudomonas syringae pv. maculicola causes systemic priming for enhanced AGO2 expression. Together our results indicate that defence priming targets the AGO2 component of RNA silencing whose enhanced expression is likely to contribute to SAR.

  12. Cucumber Mosaic Virus Infection in Arabidopsis: A Conditional Mutualistic Symbiont? International-journal

    Hideki Takahashi, Midori Tabara, Shuhei Miyashita, Sugihiro Ando, Shuichi Kawano, Yoshinori Kanayama, Toshiyuki Fukuhara, Richard Kormelink

    Frontiers in microbiology　12　770925-770925　2021

    DOI： 10.3389/fmicb.2021.770925 　

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    A cucumber mosaic virus isolate, named Ho [CMV(Ho)], was isolated from a symptomless Arabidopsis halleri field sample containing low virus titers. An analysis of CMV(Ho) RNA molecules indicated that the virus isolate, besides the usual cucumovirus tripartite RNA genome, additionally contained defective RNA3 molecules and a satellite RNA. To study the underlying mechanism of the persistent CMV(Ho) infection in perennial A. halleri, infectious cDNA clones were generated for all its genetic elements. CMV, which consists of synthetic transcripts from the infectious tripartite RNA genomes, and designated CMV(Ho)tr, multiplied in A. halleri and annual Arabidopsis thaliana Col-0 to a similar level as the virulent strain CMV(Y), but did not induce any symptoms in them. The response of Col-0 to a series of reassortant CMVs between CMV(Ho)tr and CMV(Y) suggested that the establishment of an asymptomatic phenotype of CMV(Ho) infection was due to the 2b gene of CMV RNA2, but not due to the presence of the defective RNA3 and satellite RNA. The accumulation of CMV(Ho) 2b protein tagged with the FLAG epitope (2b.Ho-FLAG) in 2b.Ho-FLAG-transformed Col-0 did not induce any symptoms, suggesting a 2b-dependent persistency of CMV(Ho)tr infection in Arabidopsis. The 2b protein interacted with Argonaute 4, which is known to regulate the cytosine methylation levels of host genomic DNA. Whole genomic bisulfite sequencing analysis of CMV(Ho)tr- and mock-inoculated Col-0 revealed that cytosine hypomethylation in the promoter regions of 82 genes, including two genes encoding transcriptional regulators (DOF1.7 and CBP1), was induced in response to CMV(Ho)tr infection. Moreover, the increased levels of hypomethylation in the promoter region of both genes, during CMV(Ho)tr infection, were correlated with the up- or down-regulation of their expression. Taken altogether, the results indicate that during persistent CMV(Ho) infection in Arabidopsis, host gene expression may be epigenetically modulated resulting from a 2b-mediated cytosine hypomethylation of host genomic DNA.

  13. Ligilactobacillus salivarius Strains Isolated From the Porcine Gut Modulate Innate Immune Responses in Epithelial Cells and Improve Protection Against Intestinal Viral-Bacterial Superinfection. International-journal

    Yuhki Indo, Shugo Kitahara, Mikado Tomokiyo, Shota Araki, Md Aminul Islam, Binghui Zhou, Leonardo Albarracin, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Takato Takenouchi, Hirohide Uenishi, Hisashi Aso, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa

    Frontiers in immunology　12　652923-652923　2021

    DOI： 10.3389/fimmu.2021.652923 　

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    Previously, we constructed a library of Ligilactobacillus salivarius strains from the intestine of wakame-fed pigs and reported a strain-dependent capacity to modulate IFN-β expression in porcine intestinal epithelial (PIE) cells. In this work, we further characterized the immunomodulatory activities of L. salivarius strains from wakame-fed pigs by evaluating their ability to modulate TLR3- and TLR4-mediated innate immune responses in PIE cells. Two strains with a remarkable immunomodulatory potential were selected: L. salivarius FFIG35 and FFIG58. Both strains improved IFN-β, IFN-λ and antiviral factors expression in PIE cells after TLR3 activation, which correlated with an enhanced resistance to rotavirus infection. Moreover, a model of enterotoxigenic E. coli (ETEC)/rotavirus superinfection in PIE cells was developed. Cells were more susceptible to rotavirus infection when the challenge occurred in conjunction with ETEC compared to the virus alone. However, L. salivarius FFIG35 and FFIG58 maintained their ability to enhance IFN-β, IFN-λ and antiviral factors expression in PIE cells, and to reduce rotavirus replication in the context of superinfection. We also demonstrated that FFIG35 and FFIG58 strains regulated the immune response of PIE cells to rotavirus challenge or ETEC/rotavirus superinfection through the modulation of negative regulators of the TLR signaling pathway. In vivo studies performed in mice models confirmed the ability of L. salivarius FFIG58 to beneficially modulate the innate immune response and protect against ETEC infection. The results of this work contribute to the understanding of beneficial lactobacilli interactions with epithelial cells and allow us to hypothesize that the FFIG35 or FFIG58 strains could be used for the development of highly efficient functional feed to improve immune health status and reduce the severity of intestinal infections and superinfections in weaned piglets.

  14. Complete genomic sequence of a novel phytopathogenic Burkholderia phage isolated from fallen leaf compost. International-journal

    Ryota Sasaki, Shuhei Miyashita, Sugihiro Ando, Kumiko Ito, Toshiyuki Fukuhara, Richard Kormelink, Hideki Takahashi

    Archives of virology　166　(1)　313-316　2021/01

    DOI： 10.1007/s00705-020-04811-3 　

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    In contrast to most Burkholderia species, which affect humans or animals, Burkholderia glumae is a bacterial pathogen of plants that causes panicle blight disease in rice seedlings, resulting in serious damage to rice cultivation. Attempts to combat this disease would benefit from research involving a phage known to attack this type of bacterium. Some Burkholderia phages have been isolated from soil or bacterial species in the order Burkholderiales, but so far there has been no report of a complete genome nucleotide sequence of a phage of B. glumae. In this study, a novel phage, FLC5, of the phytopathogen B. glumae was isolated from leaf compost, and its complete genome nucleotide sequence was determined. The genome consists of a 32,090-bp circular DNA element and exhibits a phylogenetic relationship to members of the genus Peduovirus, with closest similarity to B. multivorans phage KS14. In addition to B. glumae, FLC5 was also able to lyse B. plantarii, a pathogen causing rice bacterial damping-off disease. This is the first report of isolation of a P2-like phage from phytopathogenic Burkholderia, determination of its complete genomic sequence, and the finding of its potential to infect two Burkholderia species: B. glumae and B. plantarii.

  15. Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies. International-journal

    Binghui Zhou, Leonardo Albarracin, Yuhki Indo, Lorena Arce, Yuki Masumizu, Mikado Tomokiyo, Md Aminul Islam, Valeria Garcia-Castillo, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Hidetoshi Morita, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa

    Microorganisms　8　(11)　2020/10/26

    DOI： 10.3390/microorganisms8111659 　

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    In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different "immune" and "adhesion" phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.

  16. Priming for enhanced ARGONAUTE2 activation accompanies induced resistance to cucumber mosaic virus in Arabidopsis thaliana. International-journal

    Sugihiro Ando, Michal Jaskiewicz, Sei Mochizuki, Saeko Koseki, Shuhei Miyashita, Hideki Takahashi, Uwe Conrath

    Molecular plant pathology　22　(1)　19-30　2020/10/19

    DOI： 10.1111/mpp.13005 　

    More details Close

    Systemic acquired resistance (SAR) is a broad-spectrum disease resistance response that can be induced upon infection from pathogens or by chemical treatment, such as with benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH). SAR involves priming for more robust activation of defence genes upon pathogen attack. Whether priming for SAR would involve components of RNA silencing remained unknown. Here, we show that upon leaf infiltration of water, BTH-primed Arabidopsis thaliana plants accumulate higher amounts of mRNA of ARGONAUTE (AGO)2 and AGO3, key components of RNA silencing. The enhanced AGO2 expression is associated with prior-to-activation trimethylation of lysine 4 in histone H3 and acetylation of histone H3 in the AGO2 promoter and with induced resistance to the yellow strain of cucumber mosaic virus (CMV[Y]). The results suggest that priming A. thaliana for enhanced defence involves modification of histones in the AGO2 promoter that condition AGO2 for enhanced activation, associated with resistance to CMV(Y). Consistently, the fold-reduction in CMV(Y) coat protein accumulation by BTH pretreatment was lower in ago2 than in wild type, pointing to reduced capacity of ago2 to activate BTH-induced CMV(Y) resistance. A role of AGO2 in pathogen-induced SAR is suggested by the enhanced activation of AGO2 after infiltrating systemic leaves of plants expressing a localized hypersensitive response upon CMV(Y) infection. In addition, local inoculation of SAR-inducing Pseudomonas syringae pv. maculicola causes systemic priming for enhanced AGO2 expression. Together our results indicate that defence priming targets the AGO2 component of RNA silencing whose enhanced expression is likely to contribute to SAR.

  17. Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics. International-journal Peer-reviewed

    Asuka Tada, Akm Humayun Kober, Md Aminul Islam, Manami Igata, Michihiro Takagi, Masahiko Suzuki, Hisashi Aso, Wakako Ikeda-Ohtsubo, Kazutoyo Yoda, Kenji Miyazawa, Fang He, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Cells　9　(7)　2020/07/17

    DOI： 10.3390/cells9071715 　

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    The degree of fat accumulation and adipokine production are two major indicators of obesity that are correlated with increased adipose tissue mass and chronic inflammatory responses. Adipocytes have been considered effector cells for the inflammatory responses due to their capacity to express Toll-like receptors (TLRs). In this study, we evaluated the degree of fat accumulation and adipokine production in porcine intramuscular preadipocyte (PIP) cells maintained for in vitro differentiation over a long period without or with stimulation of either TNF-α or TLR2-, TLR3-, or TLR4-ligands. The cytosolic fat accumulation was measured by liquid chromatography and the expression of adipokines (CCL2, IL-6, IL-8 and IL-10) were quantified by RT-qPCR and ELISA at several time points (0 to 20 days) of PIP cells differentiation. Long-term adipogenic differentiation (LTAD) induced a progressive fat accumulation in the adipocytes over time. Activation of TLR3 and TLR4 resulted in an increased rate of fat accumulation into the adipocytes over the LTAD. The production of CCL2, IL-8 and IL-6 were significantly increased in unstimulated adipocytes during the LTAD, while IL-10 expression remained stable over the studied period. An increasing trend of adiponectin and leptin production was also observed during the LTAD. On the other hand, the stimulation of adipocytes with TLRs agonists or TNF-α resulted in an increasing trend of CCL2, IL-6 and IL-8 production while IL-10 remained stable in all four treatments during the LTAD. We also examined the influences of several immunoregulatory probiotic strains (immunobiotics) on the modulation of the fat accumulation and adipokine production using supernatants of immunobiotic-treated intestinal immune cells and the LTAD of PIP cells. Immunobiotics have shown a strain-specific ability to modulate the fat accumulation and adipokine production, and differentiation of adipocytes. Here, we expanded the utility and potential application of our in vitro PIP cells model by evaluating an LTAD period (20 days) in order to elucidate further insights of chronic inflammatory pathobiology of adipocytes associated with obesity as well as to explore the prospects of immunomodulatory intervention for obesity such as immunobiotics.

  18. The Role of Alveolar Macrophages in the Improved Protection against Respiratory Syncytial Virus and Pneumococcal Superinfection Induced by the Peptidoglycan of Lactobacillus rhamnosus CRL1505. International-journal

    Patricia Clua, Mikado Tomokiyo, Fernanda Raya Tonetti, Md Aminul Islam, Valeria García Castillo, Guillermo Marcial, Susana Salva, Susana Alvarez, Hideki Takahashi, Shoichiro Kurata, Haruki Kitazawa, Julio Villena

    Cells　9　(7)　2020/07/09

    DOI： 10.3390/cells9071653 　

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    The nasal priming with nonviable Lactobacillus rhamnosus CRL1505 (NV1505) or its purified peptidoglycan (PG1505) differentially modulates the respiratory innate immune response in infant mice, improving their resistance to primary respiratory syncytial virus (RSV) infection and secondary pneumococcal pneumonia. In association with the protection against RSV-pneumococcal superinfection, it was found that NV1505 or PG1505 significantly enhance the numbers of CD11c+SiglecF+ alveolar macrophages (AMs) producing interferon (IFN)-β. In this work, we aimed to further advance in the characterization of the beneficial effects of NV1505 and PG1505 in the context of a respiratory superinfection by evaluating whether their immunomodulatory properties are dependent on AM functions. Macrophage depletion experiments and a detailed study of their production of cytokines and antiviral factors clearly demonstrated the key role of this immune cell population in the improvement of both the reduction of pathogens loads and the protection against lung tissue damage induced by the immunobiotic CRL1505 strain. Studies at basal conditions during primary RSV or S. pneumoniae infections, as well as during secondary pneumococcal pneumonia, brought the following five notable findings regarding the immunomodulatory effects of NV1505 and PG1505: (a) AMs play a key role in the beneficial modulation of the respiratory innate immune response and protection against RSV infection, (b) AMs are necessary for improved protection against primary and secondary pneumococcal pneumonia, (c) the generation of activated/trained AMs would be essential for the enhanced protection against respiratory pathogens, (d) other immune and nonimmune cell populations in the respiratory tract may contribute to the protection against bacterial and viral infections, and (e) the immunomodulatory properties of NV1505 and PG1505 are strain-specific. These findings significantly improve our knowledge about the immunological mechanisms involved in the modulation of respiratory immunity induced by beneficial microbes.

  19. The Role of Alveolar Macrophages in the Improved Protection against Respiratory Syncytial Virus and Pneumococcal Superinfection Induced by the Peptidoglycan of Lactobacillus rhamnosus CRL1505

    Patricia Clua, Mikado Tomokiyo, Fernanda Raya Tonetti, Md. Aminul Islam, Valeria García Castillo, Guillermo Marcial, Susana Salva, Susana Alvarez, Hideki Takahashi, Shoichiro Kurata, Haruki Kitazawa, Julio Villena

    Cells　9　(7)　1653-1653　2020/07/09

    Publisher: MDPI AG

    DOI： 10.3390/cells9071653 　

    eISSN： 2073-4409

    More details Close

    The nasal priming with nonviable Lactobacillus rhamnosus CRL1505 (NV1505) or its purified peptidoglycan (PG1505) differentially modulates the respiratory innate immune response in infant mice, improving their resistance to primary respiratory syncytial virus (RSV) infection and secondary pneumococcal pneumonia. In association with the protection against RSV-pneumococcal superinfection, it was found that NV1505 or PG1505 significantly enhance the numbers of CD11c+SiglecF+ alveolar macrophages (AMs) producing interferon (IFN)-β. In this work, we aimed to further advance in the characterization of the beneficial effects of NV1505 and PG1505 in the context of a respiratory superinfection by evaluating whether their immunomodulatory properties are dependent on AM functions. Macrophage depletion experiments and a detailed study of their production of cytokines and antiviral factors clearly demonstrated the key role of this immune cell population in the improvement of both the reduction of pathogens loads and the protection against lung tissue damage induced by the immunobiotic CRL1505 strain. Studies at basal conditions during primary RSV or S. pneumoniae infections, as well as during secondary pneumococcal pneumonia, brought the following five notable findings regarding the immunomodulatory effects of NV1505 and PG1505: (a) AMs play a key role in the beneficial modulation of the respiratory innate immune response and protection against RSV infection, (b) AMs are necessary for improved protection against primary and secondary pneumococcal pneumonia, (c) the generation of activated/trained AMs would be essential for the enhanced protection against respiratory pathogens, (d) other immune and nonimmune cell populations in the respiratory tract may contribute to the protection against bacterial and viral infections, and (e) the immunomodulatory properties of NV1505 and PG1505 are strain-specific. These findings significantly improve our knowledge about the immunological mechanisms involved in the modulation of respiratory immunity induced by beneficial microbes.

  20. Disturbance of floral colour pattern by activation of an endogenous pararetrovirus, petunia vein clearing virus, in aged petunia plants. International-journal Peer-reviewed

    Kazunori Kuriyama, Midori Tabara, Hiromitsu Moriyama, Akira Kanazawa, Hisashi Koiwa, Hideki Takahashi, Toshiyuki Fukuhara

    The Plant journal : for cell and molecular biology　103　(2)　497-511　2020/07

    DOI： 10.1111/tpj.14728 　

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    White areas of star-type bicolour petals of petunia (Petunia hybrida) are caused by post-transcriptional gene silencing (PTGS) of the key enzyme of anthocyanin biosynthesis. We observed blotched flowers and a vein-clearing symptom in aged petunia plants. To determine the cause of blotched flowers, we focused on an endogenous pararetrovirus, petunia vein clearing virus (PVCV), because this virus may have a suppressor of PTGS (VSR). Transcripts and episomal DNAs derived from proviral PVCVs accumulated in aged plants, indicating that PVCV was activated as the host plant aged. Furthermore, DNA methylation of CG and CHG sites in the promoter region of proviral PVCV decreased in aged plants, suggesting that poor maintenance of DNA methylation activates PVCV. In parallel, de novo DNA methylation of CHH sites in its promoter region was also detected. Therefore, both activation and inactivation of PVCV occurred in aged plants. The accumulation of PVCV transcripts and episomal DNAs in blotched regions and the detection of VSR activity support a mechanism in which suppression of PTGS by PVCV causes blotched flowers.

  21. The Ability of Respiratory Commensal Bacteria to Beneficially Modulate the Lung Innate Immune Response Is a Strain Dependent Characteristic

    Ramiro Ortiz Moyano, Fernanda Raya Tonetti, Mikado Tomokiyo, Paulraj Kanmani, María Guadalupe Vizoso-Pinto, Hojun Kim, Sandra Quilodrán-Vega, Vyacheslav Melnikov, Susana Alvarez, Hideki Takahashi, Shoichiro Kurata, Haruki Kitazawa, Julio Villena

    Microorganisms　8　(5)　727-727　2020/05/13

    Publisher: MDPI AG

    DOI： 10.3390/microorganisms8050727 　

    eISSN： 2076-2607

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    We investigated whether the ability of commensal respiratory bacteria to modulate the innate immune response against bacterial and viral pathogens was a shared or strain-specific characteristic. Bacterial strains belonging to the Corynebacterium pseudodiphtheriticum and Dolosigranulum pigrum species were compared by studying their influence in the Toll-like receptor (TLR)-2- and TLR3-triggered immune responses in the respiratory tract, as well as in the resistance to Respiratory Syncytial Virus (RSV) and Streptococcus pneumoniae infections. We demonstrated that nasally administered C. pseudodiphteriticum 090104 or D. pigrum 040417 were able to modulate respiratory immunity and increase the resistance against pathogens, while other strains of the same species did not influence the respiratory immune responses, demonstrating a clear strain-dependent immunomodulatory effect of respiratory commensal bacteria. We also reported here that bacterium-like particles (BLP) and cell walls derived from immunomodulatory respiratory commensal bacteria are an interesting alternative for the modulation of the respiratory immune system. Our study is a step forward in the positioning of certain strains of respiratory commensal bacteria as next-generation probiotics for the respiratory tract.

  22. Lipoteichoic Acid Is Involved in the Ability of the Immunobiotic Strain Lactobacillus plantarum CRL1506 to Modulate the Intestinal Antiviral Innate Immunity Triggered by TLR3 Activation

    Hiroya Mizuno, Lorena Arce, Kae Tomotsune, Leonardo Albarracin, Ryutaro Funabashi, Daniela Vera, Md. Aminul Islam, Maria Guadalupe Vizoso-Pinto, Hideki Takahashi, Yasuko Sasaki, Haruki Kitazawa, Julio Villena

    Frontiers in Immunology　11　2020/04/09

    Publisher: Frontiers Media S.A.

    DOI： 10.3389/fimmu.2020.00571 　

    ISSN： 1664-3224

  23. The exopolysaccharide of Lactobacillus fermentum UCO-979C is partially involved in its immunomodulatory effect and its ability to improve the resistance against Helicobacter pylori infection Peer-reviewed

    Valeria García Castillo, Guillermo Marcial, Leonardo Albarracín, Mikado Tomokiyo, Patricia Clua, Hideki Takahashi, Haruki Kitazawa, Apolinaria Garcia Cancino, Julio Villena

    Microorganisms　2020/03

  24. Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin. International-journal Peer-reviewed

    Asuka Tada, Md Aminul Islam, Akm Humayun Kober, Kohtaro Fukuyama, Michihiro Takagi, Manami Igata, Leonardo Albarracin, Wakako Ikeda-Ohtsubo, Kenji Miyazawa, Kazutoyo Yoda, Fang He, Hideki Takahashi, Julio Villena, Hisashi Aso, Haruki Kitazawa

    International journal of molecular sciences　21　(2)　2020/01/18

    DOI： 10.3390/ijms21020638 　

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    Adipocytes are dynamic cells that have critical functions to maintain body energy homeostasis. Adipocyte physiology is affected by the adipogenic differentiation, cell program, as well as by the exogenous stimulation of biochemical factors, such as serotonin and TNF-α. In this work, we investigated the global transcriptome modifications when porcine intramuscular preadipocyte (PIP) was differentiated into porcine mature adipocyte (pMA). Moreover, we studied transcriptome changes in pMA after stimulation with serotonin or TNF-α by using a microarray approach. Transcriptome analysis revealed that the expression of 270, 261, and 249 genes were modified after differentiation, or after serotonin and TNF-α stimulation, respectively. Expression changes in APP, HNF4A, ESR1, EGR1, SRC, HNF1A, FN1, ALB, STAT3, CBL, CEBPB, AR, FOS, CFTR, PAN2, PTPN6, VDR, PPARG, STAT5A and NCOA3 genes which are enriched in the 'PPAR signaling' and 'insulin resistance' pathways were found in adipocytes during the differentiation process. Dose-dependent serotonin stimulation resulted in a decreased fat accumulation in pMAs. Serotonin-induced differentially expressed genes in pMAs were found to be involved in the significant enrichment of 'GPCR ligand-binding', 'cell chemotaxis', 'blood coagulation and complement', 'metabolism of lipid and lipoproteins', 'regulation of lipid metabolism by PPARA', and 'lipid digestion, mobilization and transport' pathways. TNF-α stimulation also resulted in transcriptome modifications linked with proinflammatory responses in the pMA of intramuscular origin. Our results provide a landscape of transcriptome modifications and their linked-biological pathways in response to adipogenesis, and exogenous stimulation of serotonin- and TNF-α to the pMA of intramuscular origin.

  25. Effect of asymptomatic infection with southern tomato virus on tomato plants. International-journal Peer-reviewed

    Toshiyuki Fukuhara, Midori Tabara, Hisashi Koiwa, Hideki Takahashi

    Archives of virology　165　(1)　11-20　2020/01

    DOI： 10.1007/s00705-019-04436-1 　

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    Southern tomato virus (STV) is often found infecting healthy tomato plants (Solanum lycopersicum). In this study, we compared STV-free and STV-infected plants of cultivar M82 to determine the effect of STV infection on the host plant. STV-free plants exhibited a short and bushy phenotype, whereas STV-infected plants were taller. STV-infected plants produced more fruit than STV-free plants, and the germination rate of seeds from STV-infected plants was higher than that of seeds from STV-free plants. This phenotypic difference was also observed in progeny plants (siblings) derived from a single STV-infected plant in which the transmission rate of STV to progeny plants via the seeds was approximately 86%. These results suggest that the interaction between STV and host plants is mutualistic. Transcriptome analysis revealed that STV infection affects gene expression in the host plant and results in downregulation of genes involved in ethylene biosynthesis and signaling. STV-infected tomato plants might thus be artificially selected due to their superior traits as a crop.

  26. Alveolar Macrophages Are Key Players in the Modulation of the Respiratory Antiviral Immunity Induced by Orally Administered Lacticaseibacillus rhamnosus CRL1505. International-journal

    Valeria Garcia-Castillo, Mikado Tomokiyo, Fernanda Raya Tonetti, Md Aminul Islam, Hideki Takahashi, Haruki Kitazawa, Julio Villena

    Frontiers in immunology　11　568636-568636　2020

    DOI： 10.3389/fimmu.2020.568636 　

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    The oral administration of Lacticaseibacillus rhamnosus CRL1505 differentially modulates the respiratory innate antiviral immune response triggered by Toll-like receptor 3 (TLR3) activation in infant mice, improving the resistance to Respiratory Syncytial Virus (RSV) infection. In this work, by using macrophages depletion experiments and a detailed study of their production of cytokines and antiviral factors we clearly demonstrated the key role of this immune cell population in the improvement of both viral elimination and the protection against lung tissue damage induced by the CRL1505 strain. Orally administered L. rhamnosus CRL1505 activated alveolar macrophages and enhanced their ability to produce type I interferons (IFNs) and IFN-γ in response to RSV infection. Moreover, an increased expression of IFNAR1, Mx2, OAS1, OAS2, RNAseL, and IFITM3 was observed in alveolar macrophages after the oral treatment with L. rhamnosus CRL1505, which was consistent with the enhanced RSV clearance. The depletion of alveolar macrophages by the time of L. rhamnosus CRL1505 administration abolished the ability of infant mice to produce increased levels of IL-10 in response to RSV infection. However, no improvement in IL-10 production was observed when primary cultures of alveolar macrophages obtained from CRL1505-treated mice were analyzed. Of note, alveolar macrophages from the CRL1505 group had an increased production of IL-6 and IL-27 suggesting that these cells may play an important role in limiting inflammation and protecting lung function during RSV infection, by increasing the maturation and activation of Treg cells and their subsequent production of IL-10. In addition, we provided evidence of the important role of CD4+ cells and IFN-γ in the activation of alveolar macrophages highlighting a putative pathway through which the intestinal and respiratory mucosa are communicated under the influence of L. rhamnosus CRL1505.

  27. Immunobiotic Lactobacillus jensenii TL2937 Alleviates Dextran Sodium Sulfate-Induced Colitis by Differentially Modulating the Transcriptomic Response of Intestinal Epithelial Cells. International-journal

    Nana Sato, Valeria Garcia-Castillo, Mao Yuzawa, Md Aminul Islam, Leonardo Albarracin, Mikado Tomokiyo, Wakako Ikeda-Ohtsubo, Apolinaria Garcia-Cancino, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Frontiers in immunology　11　2174-2174　2020

    DOI： 10.3389/fimmu.2020.02174 　

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    Immunobiotics have emerged as a promising intervention to alleviate intestinal damage in inflammatory bowel disease (IBD). However, the beneficial properties of immunobiotics are strain dependent and, therefore, each strain has to be evaluated in order to demonstrate its potential application in IBD. Our previous in vitro and in vivo studies demonstrated that Lactobacillus jensenii TL2937 attenuates gut acute inflammatory response triggered by Toll-like receptor 4 activation. However, its effect on colitis has not been evaluated before. In this work, we studied whether the TL2937 strain was able to protect against the development of colitis in a dextran sodium sulfate (DSS)-induced mouse model and we delved into the mechanisms of action by evaluating the effect of the immunobiotic bacteria on the transcriptomic response of DSS-challenged intestinal epithelial cells. L. jensenii TL2937 was administered to adult BALB/c mice before the induction of colitis by the administration of DSS. Colitis and the associated inflammatory response were evaluated for 14 days. Mice fed with L. jensenii TL2937 had lower disease activity index and alterations of colon length when compared to control mice. Reduced myeloperoxidase activity, lower production of pro-inflammatory (TNF-α, IL-1, CXCL1, MCP-1, IL-15, and IL-17), and higher levels of immunoregulatory (IL-10 and IL-27) cytokines were found in the colon of TL2937-treated mice. In addition, the treatment of porcine intestinal epithelial (PIE) cells with L. jensenii TL2937 before the challenge with DSS differentially regulated the activation of the JNK pathway, leading to an increase in epithelial cell integrity and to a differential immunotranscriptomic response. TL2937-treated PIE cells had a significant reduction in the expression of inflammatory cytokines (TNF-α, IL-1α, IL-1β, IL-6, IL-15), chemokines (CCL2, CCL4, CCL8, CXCL4, CXCL5, CXCL9, CXCL10), adhesion molecules (SELE, SELL, EPCAM), and other immune factors (NCF1, NCF2, NOS2, SAA2) when compared to control cells after the challenge with DSS. The findings of this work indicate that (a) L. jensenii TL2937 is able to alleviate DSS-induced colitis suggesting a potential novel application for this immunobiotic strain, (b) the modulation of the transcriptomic response of intestinal epithelial cells would play a key role in the beneficial effects of the TL2937 strain on colitis, and (c) the in vitro PIE cell immunoassay system could be of value for the screening and selection of new immunobiotic strains for their application in IBD.

  28. Exopolysaccharides From Streptococcus thermophilus ST538 Modulate the Antiviral Innate Immune Response in Porcine Intestinal Epitheliocytes. International-journal Peer-reviewed

    Hiroya Mizuno, Kae Tomotsune, Md Aminul Islam, Ryutaro Funabashi, Leonardo Albarracin, Wakako Ikeda-Ohtsubo, Hisashi Aso, Hideki Takahashi, Katsunori Kimura, Julio Villena, Yasuko Sasaki, Haruki Kitazawa

    Frontiers in microbiology　11　894-894　2020

    DOI： 10.3389/fmicb.2020.00894 　

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    It was reported that exopolysaccharides (EPSs) from lactobacilli are able to differentially modulate mucosal antiviral immunity. Although research has described the ability of EPSs derived from Streptococcus thermophilus to modulate the mucosal immune system, their impact on antiviral immunity was less explored. In this work, we investigated the capacity of the EPS-producing S. thermophilus ST538 to modulate the innate antiviral immune response triggered by the activation of the Toll-like receptor 3 (TLR3) in porcine intestinal epitheliocytes (PIE cells). Moreover, in order to study the immunomodulatory potential of S. thermophilus ST538 EPS, we successfully developed two mutant strains through the knockout of the epsB or epsC genes. High-performance liquid chromatography and scanning electron microscopy studies demonstrated that the wild type (WT) strain produced as high as 595 μg/ml of EPS in the skim milk medium, while none of the mutant strains (S. thermophilus ΔepsB and ΔepsC) were able to produce EPS. Studies in PIE cells demonstrated that the EPS of S. thermophilus ST538 is able to significantly improve the expression of interferon β (IFN-β), interleukin 6 (IL-6), and C-X-C motif chemokine 10 (CXCL10) in response to TLR3 stimulation. The role of EPS in the modulation of antiviral immune response in PIE cells was confirmed by comparative studies of cell free culture supernatants and fermented skim milks obtained from S. thermophilus ΔepsB and ΔepsC. These results suggest that S. thermophilus ST538 could be used as an immunobiotic strain for the development of new immunologically functional foods, which might contribute to improve resistance against viral infections.

  29. Single Amino Acid Substitutions in the Cucumber Mosaic Virus 1a Protein Induce Necrotic Cell Death in Virus-Inoculated Leaves without Affecting Virus Multiplication Peer-reviewed

    Ainan Tian, Shuhei Miyashita, Sugihiro Ando, Hideki Takahashi

    VIRUSES-BASEL　12　(1)　2020/01

    DOI： 10.3390/v12010091 　

    eISSN： 1999-4915

  30. Immunomodulatory properties of bacterium-like particles obtained from immunobiotic lactobacilli: prospects for their use as mucosal adjuvants International-journal Peer-reviewed

    Raya, F, L. Arce, S. Salva, S. Alvarez, H. Takahashi, H Kitazawa, J. Villena, M. G. Vizoso-Pinto

    Front. Immunol.　11:15　1-13　2020/01

    DOI： 10.3389/fimmu.2020.00015 　

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    IF = 4.716 国際共著論文

  31. Nasal priming with immunobiotic lactobacilli improves the adaptive immune response against influenza virus International-journal Peer-reviewed

    F. R. Tonetti, Md. A. Islam, M. G. Vizoso-Pinto, H. Takahashi, H. Kitazawa, J. Villena

    Int. Immunopharm.　78　(106115)　1-12　2019/12

    DOI： 10.1016/j.intimp.2019.106115 　

  32. Virus Latency and the Impact on Plants Peer-reviewed

    Takahashi, H, T. Fukuhara, H. Kitazawa, R. Kormelink

    Front. Microbiol.　10:2764　1-18　2019/12

    DOI： 10.3389/fmicb.2019.02764 　

  33. Exogenous application of L‐histidine suppresses bacterial diseases and enhances ethylene production in rice seedlings Peer-reviewed

    Yariyama S, Ando S, Seo S, Nakaho K, Miyashita S, Kanayama Y, Takahashi H

    Plant Pathology　68　(6)　1072-1078　2019/08

    DOI： 10.1111/ppa.13037 　

    ISSN： 0032-0862

    eISSN： 1365-3059

  34. Evaluation of the immunomodulatory activities of the probiotic strain Lactobacillus fermentum UCO-979C Peer-reviewed

    García, V, R. Komatsu, P. Clua, Y. Indo, M. Takagi, S. Salva, Md. A. Islam, S. Alvarez, H. Takahashi, A. Garcia-Cancino, H. Kitazawa, J. Villena

    Front. Immunol.　10:1376　1-14　2019/06

    DOI： 10.3389/fimmu.2019.01376 　

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    IF = 5.511 国際共著論文

  35. Transcriptome modifications in porcine adipocytes via Toll-like receptors activation Peer-reviewed

    Manami Igata, Md. Aminul Islam, Asuka Tada, Michihiro Takagi, AKM Humayun Kober, Leonardo Albarracin, Hisashi Aso, Wakako Ikeda-Ohtsubo, Kenji Miyazawa, Kazutoyo Yoda, Fang He, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Front. Immunol.　10:1180　1-15　2019/05

    DOI： 10.3389/fimmu.2019.01180 　

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    IF = 5.511 国際共著論文

  36. ヒスチジン処理した宿主植物における数種病害に対する抵抗性誘導の検討

    高橋英樹, 中保一浩, 宮下脩平, 安藤杉尋, 瀬尾茂美

    日本植物病理学会報　85　(1)　44　2019/02/25

    ISSN： 0031-9473

  37. Plant defense systems against cucumber mosaic virus: lessons learned from CMV–Arabidopsis interactions Peer-reviewed

    Sugihiro Ando, Shuhei Miyashita, Hideki Takahashi

    Journal of General Plant Pathology　85　(3)　174-181　2019/02

    DOI： 10.1007/s10327-019-00845-x 　

    ISSN： 1345-2630

    eISSN： 1610-739X

  38. Deciphering the influence of paraimmunobiotic bifidobacteria on the innate antiviral immune response of bovine intestinal epitheliocytes by transcriptomic analyasis International-journal Peer-reviewed

    Albarracin, L, R. Komatsu, V. Garcia-Castillo, H. Aso, N. Iwabuchi, J.-z. Xiao, F. Abe, H. Takahashi, J. Villena, H. Kitazawa

    Beneficial microbes　10　(2)　199-209　2019/02

    DOI： 10.3920/BM2018.0024 　

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    IF = 2.939<br /> 国際共著論文

  39. Reverse Genetic Analysis of Antiviral Resistance Signaling and the Resistance Mechanism in Arabidopsis thaliana. International-journal

    Yukiyo Sato, Hideki Takahashi

    Methods in molecular biology (Clifton, N.J.)　2028　27-84　2019

    DOI： 10.1007/978-1-4939-9635-3_3 　

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    Antiviral RNA silencing and the resistance gene-conferred defense response are major antiviral immune systems in plants. Several of the components involved have been genetically or biochemically identified in Arabidopsis thaliana. One powerful tool to dissect antiviral immune systems involves a reverse genetic approach that analyzes Arabidopsis mutant lines with impaired antiviral defense responses. In particular, to better understand the signaling networks involved in the resistance gene-conferred antiviral response in host plants, establishment of mutant lines carrying the homozygous mutant allele and antiviral resistance gene is required. The information on well-characterized defense-related signaling mutant alleles and the PCR-based genotyping method provided in this chapter allows the efficient selection of Arabidopsis mutant lines that can be used to study antiviral resistance signaling networks and resistance mechanisms.

  40. First identification and characterization of cucumber mosaic virus from Corchorus olitorius in Japan. Peer-reviewed

    Sara E Hanbal, Shuhei Miyashita, Sugihiro Ando, Samir A Sidaros, Hideki Takahashi

    Journal of Plant Pathology　100　(3)　561-565　2018/10

    DOI： 10.1007/s42161-018-0099-6 　

    ISSN： 1125-4653

  41. Receptors and signaling pathways for recognition of bacteria in livestock and crops: prospects for beneficial microbes in healthy growth strategies Peer-reviewed

    Villena, J, H. Kitazawa, S. C. M, Van Wees, C. M. J. Pieterse, H. Takahashi

    9　(2223)　1-15　2018/09

    DOI： 10.3389/fimmu.2018.02223 　

  42. Genomic characterization of Lactobacillus delbrueckii TUA4408L and evaluation of the antiviral activities of its extracellular polysaccharides in porcine intestinal epithelial cells Peer-reviewed

    Kanmani, P, L. Albarracin, H. Kobayashi, E. M. Hebert, L. Saavedra, R. Komatsu, B. Gatica, A. S. Burgos, A. Miyazaki, W. Ikeda-Ohtsubo, Y. Suda, H. Aso, S. Egusa, T. Mishima, H. Takahashi, J. Villena, H. Kitazawa

    Front. Immunol.　9　(2178)　1-16　2018/09

    DOI： 10.3389/fimmu.2018.02178 　

  43. Atmospheric-pressure plasma irradiation can disrupt tobacco mosaic virus particles and RNAs to inactivate their infectivity Peer-reviewed

    S. E Hanbal, K. Takashima, S. Miyashita, S. Ando, K. Ito, M. M Elsharkawy, T. Kaneko, H. Takahashi

    Archives of Virology　2018/06

  44. Comparative analysis of microbial diversity and bacterial seedling disease-suppressive activity in organic-farmed and standardized commercial conventional soils for rice nursery cultivation Peer-reviewed

    Takahashi, Hideki, Matsushita, Yuko, Ito, Toyoaki, Nakai, Yutaka, Nanzyo, Masami, Kobayashi, Takashi, Iwaishi, Shinji, Hashimoto, Tomoyoshi, Miyashita, Shuhei, Morikawa, Toshiyuki, Yoshida, Shigenobu, Tsushima, Seiya, Ando, Sugihiro

    JOURNAL OF PHYTOPATHOLOGY　166　(4)　249-264　2018/04

    DOI： 10.1111/jph.12682 　

    ISSN： 0931-1785

    eISSN： 1439-0434

  45. Immunobiotics for the bovine host: Their interaction with intestinal epithelial cells and their effect on antiviral immunity

    Julio Villena, Hisashi Aso, Victor P.M.G. Rutten, Hideki Takahashi, Willem van Eden, Haruki Kitazawa

    Frontiers in Immunology　9　2018/03/02

    Publisher: Frontiers Media S.A.

    DOI： 10.3389/fimmu.2018.00326 　

    ISSN： 1664-3224

  46. Survey of the response of 82 domestic landraces of Zea mays to cucumber mosaic virus (CMV) reveals geographical region‐related resistance to CMV in Japan Peer-reviewed

    Hideki Takahashi, Ainan Tian, Shuhei Miyashita, Yoshinori Kanayama, Sugihiro Ando, Richard Kormelink

    Plant Pathology　67　(6)　1401-1415　2018/03

    DOI： 10.1111/ppa.12848 　

    ISSN： 0032-0862

    eISSN： 1365-3059

  47. Increased cytosine methylation at promoter of the NB-LRR class R gene RCY1 correlated with compromised resistance to cucumber mosaic virus in EMS-generated src mutants of Arabidopsis thaliana Peer-reviewed

    Yukiyo Sato, Shuhei Miyashita, Sugihiro Ando, Hideki Takahashi

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY　100　151-162　2017/12

    DOI： 10.1016/j.pmpp.2017.09.007 　

    ISSN： 0885-5765

  48. Respiratory Commensal Bacteria Corynebacterium pseudodiphtheriticum Improves Resistance of Infant Mice to Respiratory Syncytial Virus and Streptococcus pneumoniae Superinfection Peer-reviewed

    Paulraj Kanmani, Patricia Clua, Maria G. Vizoso-Pinto, Cecilia Rodriguez, Susana Alvarez, Vyacheslav Melnikov, Hideki Takahashi, Haruki Kitazawa, Julio Villena

    FRONTIERS IN MICROBIOLOGY　8　1613　2017/08

    DOI： 10.3389/fmicb.2017.01613 　

    ISSN： 1664-302X

  49. L-Histidine Induces Resistance in Plants to the Bacterial Pathogen Ralstonia solanacearum Partially Through the Activation of Ethylene Signaling Peer-reviewed

    Shigemi Seo, Kazuhiro Nakaho, Si Won Hong, Hideki Takahashi, Hideyuki Shigemori, Ichiro Mitsuhara

    PLANT AND CELL PHYSIOLOGY　57　(9)　1932-1942　2016/09

    DOI： 10.1093/pcp/pcw114 　

    ISSN： 0032-0781

    eISSN： 1471-9053

  50. Management of bakanae and bacterial seedling blight diseases in nurseries by irradiating rice seeds with atmospheric plasma. Peer-reviewed

    Ochi, A, Konishi, H, Ando, S, Sato, K, Yokoyama, K, Tsushima, S, Yoshida, S, Morikawa, T, Kaneko, T, Takahashi, H

    Plant Pathology　65　67-76　2016

  51. Involvement of vascular hypersensitive response in quantitative resistance to Ralstonia solanacearum on tomato rootstock cultivar ‘LS-89’. Peer-reviewed

    Nakaho, K, Seo, S, Ookawa, K, Inoue, Y, Ando, S, Kanayama, Y, Miyashita, S, Takahashi, H

    Plant Pathology　65　150-158　2016

  52. 植物のR遺伝子によるウイルス抵抗性

    宮下脩平, 高橋英樹

    ウイルス　65　(2)　199-208　2015/12

    Publisher: 日本ウイルス学会

    DOI： 10.2222/jsv.65.199 　

    ISSN： 0042-6857

  53. Involvement of NtERF3 in the cell death signalling pathway mediated by SIPK/WIPK and WRKY1 in tobacco plants Peer-reviewed

    T. Ogata, H. Okada, H. Kawaide, H. Takahashi, S. Seo, I. Mitsuhara, Y. Matsushita

    PLANT BIOLOGY　17　(5)　962-972　2015/09

    DOI： 10.1111/plb.12349 　

    ISSN： 1435-8603

    eISSN： 1438-8677

  54. Cauliflower mosaic virus Tav protein induces leaf chlorosis in transgenic tobacco through a host response to virulence function of Tav Peer-reviewed

    Sumyya Waliullah, Naomi Kosaka, Takashi Yaeno, Md Emran Ali, Ken-Taro Sekine, Go Atsumi, Naoto Yamaoka, Masamichi Nishiguchi, Hideki Takahashi, Kappei Kobayashi

    JOURNAL OF GENERAL PLANT PATHOLOGY　81　(4)　261-270　2015/07

    DOI： 10.1007/s10327-015-0600-4 　

    ISSN： 1345-2630

    eISSN： 1610-739X

  55. Impact of organic crop management on suppression of bacterial seedling diseases in rice Peer-reviewed

    Sugihiro Ando, Toyoaki Ito, Takeru Kanno, Takashi Kobayashi, Toshiyuki Morikawa, Ken-Ichiro Honda, Seiya Tsushima, Hideki Takahashi

    Organic Agriculture　4　(3)　187-196　2014/10/21

    Publisher: Kluwer Academic Publishers

    DOI： 10.1007/s13165-014-0067-1 　

    ISSN： 1879-4246 1879-4238

  56. Suppressive Potential of Paenibacillus Strains Isolated from the Tomato Phyllosphere against Fusarium Crown and Root Rot of Tomato Peer-reviewed

    Ikuo Sato, Shigenobu Yoshida, Yutaka Iwamoto, Masataka Aino, Mitsuro Hyakumachi, Masafumi Shimizu, Hideki Takahashi, Sugihiro Ando, Seiya Tsushima

    MICROBES AND ENVIRONMENTS　29　(2)　168-177　2014/07

    DOI： 10.1264/jsme2.ME13172 　

    ISSN： 1342-6311

  57. Role of Intron-Mediated Enhancement on Accumulation of an Arabidopsis NB-LRR Class R-protein that Confers Resistance to Cucumber mosaic virus Peer-reviewed

    Yukiyo Sato, Sugihiro Ando, Hideki Takahashi

    PLOS ONE　9　(6)　e99041　2014/06

    DOI： 10.1371/journal.pone.0099041 　

    ISSN： 1932-6203

  58. The Temporal Evolution and Global Spread of Cauliflower mosaic virus, a Plant Pararetrovirus Peer-reviewed

    Ryosuke Yasaka, Huy D. Nguyen, Simon Y. W. Ho, Sebastian Duchene, Savas Korkmaz, Nikolaos Katis, Hideki Takahashi, Adrian J. Gibbs, Kazusato Ohshima

    PLOS ONE　9　(1)　2014/01

    DOI： 10.1371/journal.pone.0085641 　

    ISSN： 1932-6203

  59. Transcriptional profile of tomato roots exhibiting Bacillus thuringiensis-induced resistance to Ralstonia solanacearum Peer-reviewed

    Hideki Takahashi, Kazuhiro Nakaho, Takeaki Ishihara, Sugihiro Ando, Takumi Wada, Yoshinori Kanayama, Shinichiro Asano, Shigenobu Yoshida, Seiya Tsushima, Mitsuro Hyakumachi

    PLANT CELL REPORTS　33　(1)　99-110　2014/01

    DOI： 10.1007/s00299-013-1515-1 　

    ISSN： 0721-7714

    eISSN： 1432-203X

  60. WRKY70 interacting with RCY1 disease resistance protein is required for resistance to Cucumber mosaic virus in Arabidopsis thaliana Peer-reviewed

    Sugihiro Ando, Atsuya Obinata, Hideki Takahashi

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY　85　8-14　2014/01

    DOI： 10.1016/j.pmpp.2013.11.001 　

    ISSN： 0885-5765

  61. Microarray Analysis of R-Gene-Mediated Resistance to Viruses. International-journal Invited Peer-reviewed

    Ishihara, T, Sato, Y, Takahashi, H

    Plant Virology Protocols Methods in Molecular Biology　1236　197-218　2014

    DOI： 10.1007/978-1-4939-1743-3_15 　

    More details Close

    The complex process for host-plant resistance to viruses is precisely regulated by a number of genes and signaling compounds. Thus, global gene expression analysis can provide a powerful tool to grasp the complex molecular network for resistance to viruses. The procedures for comparative global gene expression profiling of virus-resistant and control plants by microarray analysis include RNA extraction, cDNA synthesis, cRNA labeling, hybridization, array scanning, and data mining steps. There are several platforms for the microarray analysis. Commercial services for the steps from cDNA synthesis to array scanning are now widely available; however, the data manipulation step is highly dependent on the experimental design and research focus. The protocols presented here are optimized for analyzing global gene expression during the R gene-conferred defense response using commercial oligonucleotide-based arrays. We also demonstrate a technique to screen for differentially expressed genes using Excel software and a simple Internet tool-based data mining approach for characterizing the identified genes.

  62. Longer uncommon polyamines have a stronger defense gene-induction activity and a higher suppressing activity of Cucumber mosaic virus multiplication compared to that of spermine in Arabidopsis thaliana Peer-reviewed

    G. H. M. Sagor, Taibo Liu, Hideki Takahashi, Masaru Niitsu, Thomas Berberich, Tomonobu Kusano

    PLANT CELL REPORTS　32　(9)　1477-1488　2013/09

    DOI： 10.1007/s00299-013-1459-5 　

    ISSN： 0721-7714

  63. Ethylene-Responsive AP2/ERF Transcription Factor MACD1 Participates in Phytotoxin-Triggered Programmed Cell Death Peer-reviewed

    Keisuke Mase, Nobuaki Ishihama, Hitoshi Mori, Hideki Takahashi, Hironori Kaminaka, Motoichiro Kodama, Hirofumi Yoshioka

    MOLECULAR PLANT-MICROBE INTERACTIONS　26　(8)　868-879　2013/08

    DOI： 10.1094/MPMI-10-12-0253-R 　

    ISSN： 0894-0282

  64. Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1 Peer-reviewed

    Mohsen Mohamed Elsharkawy, Masafumi Shimizu, Hideki Takahashi, Kouichi Ozaki, Mitsuro Hyakumachi

    PLANT PATHOLOGY JOURNAL　29　(2)　193-200　2013/06

    DOI： 10.5423/PPJ.SI.07.2012.0117 　

    ISSN： 1598-2254

  65. Bacillus thuringiensis suppresses bacterial wilt disease caused by Ralstonia solanacearum with systemic induction of defense-related gene expression in tomato Peer-reviewed

    Mitsuro Hyakumachi, Mitsuyoshi Nishimura, Tatsuyuki Arakawa, Shinichiro Asano, Shigenobu Yoshida, Seiya Tsushima, Hideki Takahashi

    Microbes and Environments　28　(1)　128-134　2013

    DOI： 10.1264/jsme2.ME12162 　

    ISSN： 1342-6311 1347-4405

  66. The plant growth-promoting fungus Fusarium equiseti and the arbuscular mycorrhizal fungus Glomus mosseae induce systemic resistance against Cucumber mosaic virus in cucumber plants Peer-reviewed

    Mohsen Mohamed Elsharkawy, Masafumi Shimizu, Hideki Takahashi, Mitsuro Hyakumachi

    PLANT AND SOIL　361　(1-2)　397-409　2012/12

    DOI： 10.1007/s11104-012-1255-y 　

    ISSN： 0032-079X

  67. Broad-spectrum resistance of Arabidopsis C24 to downy mildew is mediated by different combinations of isolate-specific loci Peer-reviewed

    Dmitry Lapin, Rhonda C. Meyer, Hideki Takahashi, Ulrike Bechtold, Guido Van den Ackerveken

    NEW PHYTOLOGIST　196　(4)　1171-1181　2012/12

    DOI： 10.1111/j.1469-8137.2012.04344.x 　

    ISSN： 0028-646X

  68. Induction of systemic resistance against Cucumber mosaic virus by Penicillium simplicissimum GP17-2 in Arabidopsis and tobacco Peer-reviewed

    M. M. Elsharkawy, M. Shimizu, H. Takahashi, M. Hyakumachi

    PLANT PATHOLOGY　61　(5)　964-976　2012/10

    DOI： 10.1111/j.1365-3059.2011.02573.x 　

    ISSN： 0032-0862

    eISSN： 1365-3059

  69. Role of Vacuolar H+-inorganic pyrophosphatase in tomato fruit development. Peer-reviewed

    Mohammed SA, Nishio S, Takahashi H, Shiratake K, Ikeda H, Kanahama K, Kanayama Y

    Journal of experimental botany　63　(15)　5613-5621　2012/09

    DOI： 10.1093/jxb/ers213 　

    ISSN： 0022-0957

  70. RCY1-Mediated Resistance to Cucumber mosaic virus Is Regulated by LRR Domain-Mediated Interaction with CMV(Y) Following Degradation of RCY1 Peer-reviewed

    Hideki Takahashi, Hiromi Shoji, Sugihiro Ando, Yoshinori Kanayama, Tomonobu Kusano, Minoru Takeshita, Masashi Suzuki, Chikara Masuta

    MOLECULAR PLANT-MICROBE INTERACTIONS　25　(9)　1171-1185　2012/09

    DOI： 10.1094/MPMI-04-12-0076-R 　

    ISSN： 0894-0282

  71. Cyclic nucleotide-gated ion channel-mediated cell death may not be critical for R gene-conferred resistance to Cucumber mosaic virus in Arabidopsis thaliana Peer-reviewed

    Hideki Takahashi, Asako Kai, Masao Yamashita, Sugihiro Ando, Ken-Taro Sekine, Yoshinori Kanayama, Hirofumi Tomita

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY　79　40-48　2012/07

    DOI： 10.1016/j.pmpp.2012.03.003 　

    ISSN： 0885-5765

  72. Exogenous thermospermine has an activity to induce a subset of the defense genes and restrict cucumber mosaic virus multiplication in Arabidopsis thaliana Peer-reviewed

    G. H. M. Sagor, Hideki Takahashi, Masaru Niitsu, Yoshihiro Takahashi, Thomas Berberich, Tomonobu Kusano

    PLANT CELL REPORTS　31　(7)　1227-1232　2012/07

    DOI： 10.1007/s00299-012-1243-y 　

    ISSN： 0721-7714

  73. Biocontrol of black scurf on potato by seed tuber treatment with Pythium oligandrum Peer-reviewed

    Sachiko Ikeda, Ayano Shimizu, Motoshige Shimizu, Hideki Takahashi, Shigehito Takenaka

    BIOLOGICAL CONTROL　60　(3)　297-304　2012/03

    DOI： 10.1016/j.biocontrol.2011.10.016 　

    ISSN： 1049-9644

  74. Transcriptome analysis of quantitative resistance-specific response upon Ralstonia solanacearum infection in tomato. Peer-reviewed

    Ishihara T, Mitsuhara I, Takahashi H, Nakaho K

    PloS one　7　(10)　e46763　2012

    DOI： 10.1371/journal.pone.0046763 　

    ISSN： 1932-6203

  75. Transcriptome analysis in resistant and susceptible cultivars of tomato infected with Ralstonia solanacearum. Peer-reviewed

    Ishihara, T, Mitsuhara, I, Takahashi, H, Nakaho, K

    PLoS One　7　e46763　2012

    DOI： 10.1371/journal.pone.0046763 　

  76. Implications of oligomeric forms of POD-1 and POD-2 proteins isolated from cell walls of the biocontrol agent Pythium oligandrum in relation to their ability to induce defense reactions in tomato Peer-reviewed

    Shigehito Takenaka, Keishi Yamaguchi, Akira Masunaka, Syu Hase, Tsuyoshi Inoue, Hideki Takahashi

    JOURNAL OF PLANT PHYSIOLOGY　168　(16)　1972-1979　2011/11

    DOI： 10.1016/j.jplph.2011.05.011 　

    ISSN： 0176-1617

  77. Suppression of telomere-binding protein gene expression represses seed and fruit development in tomato Peer-reviewed

    Ryo Moriguchi, Kensuke Ohata, Koki Kanahama, Hideki Takahashi, Manabu Nishiyama, Yoshinori Kanayama

    JOURNAL OF PLANT PHYSIOLOGY　168　(16)　1927-1933　2011/11

    DOI： 10.1016/j.jplph.2011.05.009 　

    ISSN： 0176-1617

  78. Microbial community profiles in intercellular fluid of rice Peer-reviewed

    Hideki Takahashi, Hiroyuki Sekiguchi, Toyoaki Ito, Masashi Sasahara, Noriko Hatanaka, Atsushi Ohba, Shu Hase, Sugihiro Ando, Hiroshi Hasegawa, Shigehito Takenaka

    JOURNAL OF GENERAL PLANT PATHOLOGY　77　(2)　121-131　2011/03

    DOI： 10.1007/s10327-010-0289-3 　

    ISSN： 1345-2630

  79. CRT1, a new player in R (resistance) gene-mediated immunity, implicates endosomes in defense signaling. Peer-reviewed

    Hong-Gu Kang, Chang-Sik Oh, Masanao Sato, Fumiaki Katagiri, Jane Glazebrook, Hideki Takahashi, Gregory Martin, Daniel F. Klessig

    Plant Cell　2010/09

  80. Expression analysis of the auxin efflux carrier family in tomato fruit development Peer-reviewed

    Sogo Nishio, Ryo Moriguchi, Hiroki Ikeda, Hideki Takahashi, Hideyuki Takahashi, Nobuharu Fujii, Thomas J. Guilfoyle, Koki Kanahama, Yoshinori Kanayama

    PLANTA　232　(3)　755-764　2010/08

    DOI： 10.1007/s00425-010-1211-0 　

    ISSN： 0032-0935

  81. Distribution and Expression of Elicitin-like Protein Genes of the Biocontrol Agent Pythium oligandrum Peer-reviewed

    Akira Masunaka, Hiroyuki Sekiguchi, Hideki Takahashi, Shigehito Takenaka

    JOURNAL OF PHYTOPATHOLOGY　158　(6)　417-426　2010/06

    DOI： 10.1111/j.1439-0434.2009.01641.x 　

    ISSN： 0931-1785

  82. Endosome-Associated CRT1 Functions Early in Resistance Gene-Mediated Defense Signaling in Arabidopsis and Tobacco Peer-reviewed

    Hong-Gu Kang, Chang-Sik Oh, Masanao Sato, Fumiaki Katagiri, Jane Glazebrook, Hideki Takahashi, Pradeep Kachroo, Gregory B. Martin, Daniel F. Klessig

    PLANT CELL　22　(3)　918-936　2010/03

    DOI： 10.1105/tpc.109.071662 　

    ISSN： 1040-4651

  83. Large-scale analysis of full-length cDNAs from the tomato (Solanum lycopersicum) cultivar Micro-Tom, a reference system for the Solanaceae genomics Peer-reviewed

    Koh Aoki, Kentaro Yano, Ayako Suzuki, Shingo Kawamura, Nozomu Sakurai, Kunihiro Suda, Atsushi Kurabayashi, Tatsuya Suzuki, Taneaki Tsugane, Manabu Watanabe, Kazuhide Ooga, Maiko Torii, Takanori Narita, Tadasu Shin-i, Yuji Kohara, Naoki Yamamoto, Hideki Takahashi, Yuichiro Watanabe, Mayumi Egusa, Motoichiro Kodama, Yuki Ichinose, Mari Kikuchi, Sumire Fukushima, Akiko Okabe, Tsutomu Arie, Yuko Sato, Katsumi Yazawa, Shinobu Satoh, Toshikazu Omura, Hiroshi Ezura, Daisuke Shibata

    BMC GENOMICS　11　2010/03

    DOI： 10.1186/1471-2164-11-210 　

    ISSN： 1471-2164

  84. Identification of a Protein that Interacts with LeATL6 Ubiquitin-protein Ligase E3 Upregulated in Tomato Treated with Elicitin-Like Cell Wall Proteins of Pythium oligandrum Peer-reviewed

    Hideki Takahashi, Shu Hase, Yoshinori Kanayama, Shigehito Takenaka

    JOURNAL OF PHYTOPATHOLOGY　158　(2)　132-136　2010/02

    DOI： 10.1111/j.1439-0434.2009.01589.x 　

    ISSN： 0931-1785

  85. Visualization of Ralstonia solanacearum cells during biocontrol of bacterial wilt disease in tomato with Pythium oligandrum Peer-reviewed

    Akira Masunaka, Kazuhiro Nakaho, Masao Sakai, Hideki Takahashi, Shigehito Takenaka

    JOURNAL OF GENERAL PLANT PATHOLOGY　75　(4)　281-287　2009/08

    DOI： 10.1007/s10327-009-0173-1 　

    ISSN： 1345-2630

  86. INF1 Elicitin Activates Jasmonic Acid- and Ethylene-mediated Signalling Pathways and Induces Resistance to Bacterial Wilt Disease in Tomato Peer-reviewed

    Yoko Kawamura, Shu Hase, Shigehito Takenaka, Yoshinori Kanayama, Hirofumi Yoshioka, Sophien Kamoun, Hideki Takahashi

    JOURNAL OF PHYTOPATHOLOGY　157　(5)　287-297　2009/05

    DOI： 10.1111/j.1439-0434.2008.01489.x 　

    ISSN： 0931-1785

  87. Enhanced Defense Responses in Arabidopsis Induced by the Cell Wall Protein Fractions from Pythium oligandrum Require SGT1, RAR1, NPR1 and JAR1 Peer-reviewed

    Yoko Kawamura, Shigehito Takenaka, Shu Hase, Mayumi Kubota, Yuki Ichinose, Yoshinori Kanayama, Kazuhiro Nakaho, Daniel F. Klessig, Hideki Takahashi

    PLANT AND CELL PHYSIOLOGY　50　(5)　924-934　2009/05

    DOI： 10.1093/pcp/pcp044 　

    ISSN： 0032-0781

    eISSN： 1471-9053

  88. Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes Peer-reviewed

    Aki Igarashi, Kousuke Yamagata, Tomokazu Sugai, Yukari Takahashi, Emiko Sugawara, Akihiro Tamura, Hajime Yaegashi, Noriko Yamagishi, Tsubasa Takahashi, Masamichi Isogai, Hideki Takahashi, Nobuyuki Yoshikawa

    VIROLOGY　386　(2)　407-416　2009/04

    DOI： 10.1016/j.virol.2009.01.039 　

    ISSN： 0042-6822

  89. Spermine signaling plays a significant role in the defense response of Arabidopsis thaliana to cucumber mosaic virus Peer-reviewed

    Yoshiko Mitsuya, Yoshihiro Takahashi, Thomas Berberich, Atsushi Miyazaki, Hideo Matsumura, Hideki Takahashi, Ryohei Terauchi, Tomonobu Kusano

    JOURNAL OF PLANT PHYSIOLOGY　166　(6)　626-643　2009

    DOI： 10.1016/j.jplph.2008.08.006 　

    ISSN： 0176-1617

  90. High Level Expression of a Virus Resistance Gene, RCY1, Confers Extreme Resistance to Cucumber mosaic virus in Arabidopsis thaliana Peer-reviewed

    Ken-Taro Sekine, Sayaka Kawakami, Shu Hase, Mayumi Kubota, Yuki Ichinose, Jyoti Shah, Hong-Gu Kang, Daniel F. Klessig, Hideki Takahashi

    MOLECULAR PLANT-MICROBE INTERACTIONS　21　(11)　1398-1407　2008/11

    DOI： 10.1094/MPMI-21-11-1398 　

    ISSN： 0894-0282

  91. Involvement of jasmonic acid signalling in bacterial wilt disease resistance induced by biocontrol agent Pythium oligandrum in tomato Peer-reviewed

    S. Hase, S. Takahashi, S. Takenaka, K. Nakaho, T. Arie, S. Seo, Y. Ohashi, H. Takahashi

    PLANT PATHOLOGY　57　(5)　870-876　2008/10

    DOI： 10.1111/j.1365-3059.2008.01858.x 　

    ISSN： 0032-0862

  92. Overexpression of the Arabidopsis thaliana EDS5 gene enhances resistance to viruses Peer-reviewed

    T. Ishihara, K. -T. Sekine, S. Hase, Y. Kanayama, S. Seo, Y. Ohashi, T. Kusano, D. Shibata, J. Shah, H. Takahashi

    PLANT BIOLOGY　10　(4)　451-461　2008/07

    DOI： 10.1111/j.1438-8677.2008.00050.x 　

    ISSN： 1435-8603

  93. Colonization of Pythium oligandrum in the tomato rhizosphere for biological control of bacterial wilt disease analyzed by real-time PCR and confocal laser-scanning microscopy Peer-reviewed

    Shigehito Takenaka, Hiroyuki Sekiguchi, Kazuhiro Nakaho, Motoaki Tojo, Akira Masunaka, Hideki Takahashi

    PHYTOPATHOLOGY　98　(2)　187-195　2008/02

    DOI： 10.1094/PHYTO-98-2-0187 　

    ISSN： 0031-949X

  94. Veinal necrosis induced by Turnip mosaic virus infection in Arabidopsis is a form of defense response accompanying HR-like cell death Peer-reviewed

    Bomin Kim, Chikara Masuta, Hideyuki Matsuura, Hideki Takahashi, Tsuyoshi Inukai

    MOLECULAR PLANT-MICROBE INTERACTIONS　21　(2)　260-268　2008/02

    DOI： 10.1094/MPMI-21-2-0260 　

    ISSN： 0894-0282

  95. Identification of a novel Cys(2)/His(2)-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco Peer-reviewed

    Yoshiko Mitsuya, Yoshihiro Takahashi, Yukiko Uehara, Thomas Berberich, Atsushi Miyazaki, Hideki Takahashi, Tomonobu Kusano

    JOURNAL OF PLANT PHYSIOLOGY　164　(6)　785-793　2007/06

    DOI： 10.1016/j.jplph.2006.05.011 　

    ISSN： 0176-1617

  96. Up-regulation of LeATL6 that encodes a fungal elicitor-responsive ubiquitin ligase induces jasmonic acid-dependent proteinase inhibitor gene expression in tomato Peer-reviewed

    Hondo, D, Hase, S, Kanayama, Y, Yoshikawa, N, Takenaka, S, Takahashi, H

    Molecular Plant-Microbe Interactions　20　72-81　2007

    DOI： 10.1094/MPMI-20-0072 　

    More details Close

    IF 3.936

  97. Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death Peer-reviewed

    Ken-Taro Sekine, Takeaki Ishihara, Shu Hase, Tomonobu Kusano, Jyoti Shah, Hideki Takahashi

    PLANT MOLECULAR BIOLOGY　62　(4-5)　669-682　2006/11

    DOI： 10.1007/s11103-006-9048-4 　

    ISSN： 0167-4412

  98. Novel elicitin-like proteins isolated from the cell wall of the biocontrol agent Pythium oligandrum induce defence-related genes in sugar beet Peer-reviewed

    Shigehito Takenaka, Yumi Nakamura, Tamaki Kono, Hiroyuki Sekiguchi, Akira Masunaka, Hideki Takahashi

    MOLECULAR PLANT PATHOLOGY　7　(5)　325-339　2006/09

    DOI： 10.1111/j.1364-3703.2006.00340.x 　

    ISSN： 1464-6722

    eISSN： 1364-3703

  99. Beta-cyanoalanine synthase as a molecular marker for induced resistance by fungal glycoprotein elicitor and commercial plant activators Peer-reviewed

    Hideki Takahashi, Takeaki Ishihara, Shu Hase, Ayaka Chiba, Kazuhiro Nakaho, Tsutomu Arie, Tohru Teraoka, Michiaki Iwata, Taneaki Tugane, Daisuke Shibata, Shigehito Takenaka

    PHYTOPATHOLOGY　96　(8)　908-916　2006/08

    DOI： 10.1094/PHYTO-96-0908 　

    ISSN： 0031-949X

    eISSN： 1943-7684

  100. Induction of transient ethylene and reduction in severity of tomato bacterial wilt by Pythium oligandrum Peer-reviewed

    S. Hase, A. Shimizu, K. Nakaho, S. Takenaka, H. Takahashi

    PLANT PATHOLOGY　55　(4)　537-543　2006/08

    DOI： 10.1111/j.1365-3059.2006.01396.x 　

    ISSN： 0032-0862

  101. Pythium oligandrum induces transient ethylene production and reduces the severity of bacterial wilt disease caused by Ralstonia solanacearum in tomato.

    Hase, S, Shimizu, A, Nakaho, K, Takenaka, S, Takahashi, H

    Plant Pathol.　55　537-543　2006

  102. Identification of the cis-acting elements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment Peer-reviewed

    MS Zheng, H Takahashi, A Miyazaki, K Yamaguchi, T Kusano

    PLANT SCIENCE　168　(2)　415-422　2005/02

    DOI： 10.1016/j.plantsci.2004.09.016 　

    ISSN： 0168-9452

  103. Catalog of Micro-Tom tomato responses to common fungal, bacterial, and viral pathogens Peer-reviewed

    Hideki Takahashi, Ayano Shimizu, Tsutomu Arie, Syofi Rosmalawati, Sumire Fukushima, Mari Kikuchi, Yasufumi Hikichi, Ayami Kanda, Akiko Takahashi, Akinori Kiba, Kohei Ohnishi, Yuki Ichinose, Fumiko Taguchi, Chihiro Yasuda, Motoichiro Kodama, Mayumi Egusa, Chikara Masuta, Hiroyuki Sawada, Daisuke Shibata, Koichi Hori, Yuichiro Watanabe

    Journal of General Plant Pathology　71　(1)　8-22　2005/02

    DOI： 10.1007/s10327-004-0168-x 　

    ISSN： 1345-2630

  104. Tobacco ZFT1, a transcriptional repressor with a Cys2/His2 type zinc finger motif that functions in spermine-signaling pathway Peer-reviewed

    Uehara, Y, Takahashi, Y, Berberich, T, Miyazaki, A, Takahashi, H, Matsui, K, Ohme-Takagi, M, Saitoh, H, Terauchi, R, Kusano, T

    Plant Molecular Biology　59　433-446　2005

    DOI： 10.1007/s11103-005-0272-0 　

    More details Close

    IF 3.577

  105. A subset of hypersensitive response marker genes, including HSR203J, is the downstream target of a spermine signal transduction pathway in tobacco Peer-reviewed

    Y Takahashi, Y Uehara, T Berberich, A Ito, H Saitoh, A Miyazaki, R Terauchi, T Kusano

    PLANT JOURNAL　40　(4)　586-595　2004/11

    DOI： 10.1111/j.1365-313X.2004.02234.x 　

    ISSN： 0960-7412

  106. Enhanced resistance to Cucumber mosaic virus in the Arabidopsis thaliana ssi2 mutant is mediated via an SA-independent mechanism Peer-reviewed

    KT Sekine, A Nandi, T Ishihara, S Hase, M Ikegami, J Shah, H Takahashi

    MOLECULAR PLANT-MICROBE INTERACTIONS　17　(6)　623-632　2004/06

    ISSN： 0894-0282

  107. Antagonistic interactions between the SA and JA signaling pathways in Arabidopsis modulate expression of defense genes and gene-for-gene resistance to cucumber mosaic virus Peer-reviewed

    H Takahashi, Y Kanayama, MS Zheng, T Kusano, S Hase, M Ikegami, J Shah

    PLANT AND CELL PHYSIOLOGY　45　(6)　803-809　2004/06

    DOI： 10.1093/pcp/pch085 　

    ISSN： 0032-0781

  108. Up-regulation of Arabidopsis thaliana NHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signalling pathways that differ in salicylate involvement Peer-reviewed

    MS Zheng, H Takahashi, A Miyazaki, H Hamamoto, J Shah, Yamaguchi, I, T Kusano

    PLANTA　218　(5)　740-750　2004/03

    DOI： 10.1007/s00425-003-1169-2 　

    ISSN： 0032-0935

  109. Comparative analysis of expressed sequence tags in resistant and susceptible ecotypes of Arabidopsis thaliana infected with cucumber mosaic virus Peer-reviewed

    T Ishihara, N Sakurai, KT Sekine, S Hase, M Ikegami, D Shibata, H Takahashi

    PLANT AND CELL PHYSIOLOGY　45　(4)　470-480　2004

    DOI： 10.1093/pcp/pch057 　

    ISSN： 0032-0781

  110. Molecular Characterization of a Strain of Squash Leaf Curl China Virus from the Philippines Peer-reviewed

    T. Kon, L. M. Dolores, N. B. Bajet, S. Hase, H. Takahashi, M. Ikegami

    Journal of Phytopathology　151　(10)　535-539　2003/10

    DOI： 10.1046/j.1439-0434.2003.00764.x 　

    ISSN： 0931-1785

  111. Activation of defense-related gene expression and systemic acquired resistance in cucumber mosaic virus-infected tobacco plants expressing the mammalian 2 ' 5 ' oligoadenylate system - Brief report Peer-reviewed

    A Honda, H Takahashi, T Toguri, T Ogawa, S Hase, M Ikegami, Y Ehara

    ARCHIVES OF VIROLOGY　148　(5)　1017-1026　2003/05

    DOI： 10.1007/s00705-002-0959-3 　

    ISSN： 0304-8608

  112. Genome organization of an infectious clone of Tomato leaf curl virus (Philippines), a new monopartite Begomovirus Peer-reviewed

    Tatsuya Kon, Lolita M. Dolores, Akiko Murayama, Narceo B. Bajet, Shu Hase, Hideki Takahashi, Masato Ikegami

    Journal of Phytopathology　150　(11-12)　587-591　2002/12

    DOI： 10.1046/j.1439-0434.2002.00817.x 　

    ISSN： 0931-1785

  113. RCY1, an Arabidopsis thaliana RPP8/HRT family resistance gene, conferring resistance to cucumber mosaic virus requires salicylic acid, ethylene and a novel signal transduction mechanism Peer-reviewed

    H Takahashi, J Miller, Y Nozaki, Sukamto, M Takeda, J Shah, S Hase, M Ikegami, Y Ehara, SP Dinesh-Kumar

    PLANT JOURNAL　32　(5)　655-667　2002/12

    DOI： 10.1046/j.1365-313X.2002.01453.x 　

    ISSN： 0960-7412

  114. Ultrastructure of necrotic lesions in Nicotiana glutinosa leaves locally infected with a variant of Cucumber mosaic virus Peer-reviewed

    T. Ishihara, H. Takahashi, S. Hase, T. Sato, M. Ikegami, Y. Ehara

    Journal of Phytopathology　150　(10)　553-556　2002/10

    DOI： 10.1046/j.1439-0434.2002.00802.x 　

    ISSN： 0931-1785

  115. The genome organization of pea stem necrosis virus and its assignment to the genus Carmovirus Peer-reviewed

    S Suzuki, S Hase, H Takahashi, M Ikegami

    INTERVIROLOGY　45　(3)　160-163　2002/05

    DOI： 10.1159/000065870 　

    ISSN： 0300-5526

  116. Phosphorylation of the movement protein of Cucumber mosaic virus in transgenic tobacco plants Peer-reviewed

    Y. Matsushita, K. Yoshioka, T. Shigyo, H. Takahashi, H. Nyunoya

    Virus Genes　24　(3)　231-234　2002

    DOI： 10.1023/A:1015324415110 　

    ISSN： 0920-8569

  117. Mapping the virus and host genes involved in the resistance response in cucumber mosaic virus-infected Arabidopsis thaliana Peer-reviewed

    Hideki Takahashi, Masashi Suzuki, Keiko Natsuaki, Takuma Shigyo, Kumiko Hino, Tohru Teraoka, Daijiro Hosokawa, Yoshio Ehara

    Plant and Cell Physiology　42　(3)　340-347　2001

    Publisher: Japanese Society of Plant Physiologists

    DOI： 10.1093/pcp/pce039 　

    ISSN： 0032-0781

  118. Isolation of molecular markers linked to the Cry locus conferring resistance to Cucumber mosaic cucumovirus infection in cowpea. Peer-reviewed

    Chida, Y, Okazaki, K, Karasawa, A, Akashi, K, Nakazawa-Nasu, Y, Hase, S, Takahashi, H, Ehara, Y

    Journal of General Plant Pathology　66　(3)　242-250　2000/08

    Publisher: Phytopathological Society of Japan

    DOI： 10.1007/PL00012953 　

    ISSN： 1345-2630

    More details Close

    We previously demonstrated that cowpea[Vigna unguiculata(L.)Walp.]cultivar Kurodane-Sanjaku contains the Cry gene, which confers resistance against Cucumber mosaic cucumovirus infection. In this paper, randomly amplified polymorphic DNA(RAPD)analysis was carried out to tag the Cry locus. Bulked segregant analysis for RAPD resulted in many polymorphisms in amplified DNA patterns. Candidates were further screened using parental and/or F_2 cowpea DNAs. As a result, we obtained three RAPD markers, D13/E14-350, WA3-850 and OPE3-500, flanking the Cry locus. In addition, we amplified cowpea sequences coding for the putative nucleotide-binding site(NBS). Degenerate primers based on NBS sequences of tobacco N and Arabidopsis RPS2 disease resistance genes were used for polymerase chain reaction, and resultant products were cloned and sequenced. Among eight independent clones, cowpea resistance gene analog(CRGA)5 showed a distinct polymorphism when used as a probe for restriction fragment length polymorphism analysis against the susceptible cowpea cultivar PI 189375 and a near-isogenic line for the Cry. Linkage analyses of these molecular markers showed that genetic distances of CRGA5, D13/E14-350, WA3-850 and OPE3-500 to the Cry locus were 0.7, 5.2, 11.5 and 24.5cM, respectively.

  119. A variant of cucumber mosaic virus is restricted to local lesions in inoculated tobacco leaves with a hypersensitive response Peer-reviewed

    Takahashi, H, Sugiyama, M, Sukamto, Karasawa, A, Hase, S, Ehara, Y

    J.Ger.Plant Pathol.　66　(4)　335-344　2000/05

    Publisher: Phytopathological Society of Japan

    DOI： 10.1007/PL00012975 　

    ISSN： 1345-2630

    More details Close

    A variant of Cucumber mosaic virus, CMV (Y/GM2), was isolated from a tobacco plant with mild green mosaic symptoms that was regenerated in vitro from a yellow strain of CMV [CMV(Y)] -infected tobacco leaves by tissue culture. CMV (Y/GM2) has two amino acid substitutions at 36 and 111 positions in the coat protein encoded on RNA3. CMV, assembled by mixing in vitro transcribed CMV (Y) RNA1 and RNA2 plus infectious RNA3 transcribed in vitro from cDNA to RNA3 of CMV (Y/GM2), was prepared and designated as CMV (Y/GM2) tr. When tobacco (Nicotiana tabacum cv. Xanthi nc) plants were inoculated with CMV (Y/GM2) tr, large necrotic local lesions in which the virus was localized, developed on the inoculated leaves. This host response unique to CMV (Y/GM2) tr was similar to the hypersensitive response (HR), which is a common resistance response to avirulent pathogens and was observed in five cultivars of Nicotiana tabacum and eight Nicotiana species. The revertant virus, however, accumulated to quite different levels in the various hosts. CMV (Y/GM2) tr induced pathogenesis-related 1 (PR-1) protein accumulation and systemic acquired resistance (SAR) which were generally observed in the HR. However, when tobaccos were inoculated with CMV (S36P) tr and CMV (V111I) tr, which have an amino acid substitution at either the 36 or 111 position in the coat protein of CMV (Y), respectively, CMV (S36P) tr was restricted to the primary infection site without necrotic local lesion formation and PR-1 protein and SAR induction. CMV (V111I) tr, however, systemically spread and induced mild green mosaic symptoms, while the host had the HR to CMV (Y/GM2) tr. The localization on CMV (Y/GM2) tr at the primary infection site may not only be caused by the HR, but also by the restriction of virus systemic movement resulting from the amino acid substitution at position 36 in the coat protein of CMV (Y).

  120. A novel satellite RNA of cucumber mosaic virus induces unique line-pattern mosaic symptoms in tobacco Peer-reviewed

    H. Sato, S. Hase, M. Sugiyama, A. Karasawa, T. Suzuki, H. Takahashi, Y. Ehara

    Journal of Phytopathology　148　(1)　47-51　2000

    DOI： 10.1046/j.1439-0434.2000.00469.x 　

    ISSN： 0931-1785

  121. Characterization of symptom determinants in two mutants of cucumber mosaic virus Y strain, causing distinct mild green mosaic symptoms in tobacco Peer-reviewed

    M. Sugiyama, H. Sato, A. Karasawa, S. Hase, H. Takahashi, Y. Ehara

    Physiological and Molecular Plant Pathology　56　(2)　85-90　2000

    Publisher: Academic Press

    DOI： 10.1006/pmpp.1999.0250 　

    ISSN： 0885-5765

  122. Novel mannose-binding rice lectin composed of some isolectins and its relation to a stress-inducible salT gene Peer-reviewed

    Ko Hirano, Tohru Teraoka, Homare Yamanaka, Akane Harashima, Akiko Kunisaki, Hideki Takahashi, Daijiro Hosokawa

    Plant and Cell Physiology　41　(3)　258-267　2000

    Publisher: Japanese Society of Plant Physiologists

    DOI： 10.1093/pcp/41.3.258 　

    ISSN： 0032-0781

  123. Development of necrosis and activation of disease resistance in transgenic tobacco plants with severely reduced catalase levels Peer-reviewed

    Hideki Takahashi, Zhixiang Chen, He Du, Yidong Liu, Daniel F. Klessig

    Plant Journal　11　(5)　993-1005　1997/05

    DOI： 10.1046/j.1365-313X.1997.11050993.x 　

    ISSN： 0960-7412

  124. Studies of the salicylic acid signal transduction pathway Peer-reviewed

    DF Klessig, J Durner, ZX Chen, M Anderson, U Conrath, H Du, A Guo, YD Liu, J Shah, H Silva, H Takahashi, YN Yang

    BIOLOGY OF PLANT-MICROBE INTERACTIONS　33-38　1996

  125. Studies on Mechanisms of Symptom Expression on Cucumber Mosaic Virus Disease

    TAKAHASHI Hideki

    Annals of the Phytopathological Society of Japan　60　(3)　280-280　1994/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    DOI： 10.3186/jjphytopath.60.280 　

    ISSN： 0031-9473

  126. Hypersensitive response in cucumber mosaic virus‐inoculated Arabidopsis thaliana Peer-reviewed

    Hideki Takahashi, Nobuharu Goto, Yoshio Ehara

    The Plant Journal　6　(3)　369-377　1994

    DOI： 10.1046/j.1365-313X.1994.06030369.x 　

    ISSN： 1365-313X 0960-7412

  127. Severe Chlorotic spot symptoms in cucumber mosaic virus strain Y-infected tobaccos are induced by a combination of the virus coat protein gene and two host recessive genes Peer-reviewed

    Takahashi, H, Ehara, Y

    Mol. Plant-Microbe Interact.　6　182-189　1993/12

  128. Changes in the activity and the polypeptide composition of the oxygen-evolving complex in photosystem II of tobacco leaves infected with cucumber mosaic virus strain Y Peer-reviewed

    Takahashi, H, Ehara, Y

    Mol. Plant-Microbe Interact.　5　269-272　1992/12

  129. Poly(A)-binding material from higher plant cells does not affect in vitro translation of messenger ribonucleic acid

    Nitta, T, Takahashi, H

    Bull. Fac. Gen. Ed. Tokyo Univ. Agri. Tech.　28　155-157　1992/10

  130. Nucleotide sequence of RNA2 of Cucumber mosaic virus strain O Peer-reviewed

    Hase, S, Karasawa, A, Takahashi, H, Ehara, Y

    Annals of the Phytopathological Society of Japan　58　(5)　789-793　1992/01

    Publisher: The Phytopathological Society of Japan

    DOI： 10.3186/jjphytopath.58.789 　

    ISSN： 0031-9473

  131. A protein in the oxygen-evolving complex in the chloroplast is associated with symptom expression on tobacco leaves infected with cucumber mosaic virus strain Y Peer-reviewed

    Takahashi, H, Ehara, Y, Hirano, H

    Plant Mol. Biol.　(16)　689-698　1991/12

  132. Cauliflower mosaic virus gene VI canses growth suppression, development of defence-related gene in transgenic tobacco plants Peer-reviewed

    Takahashi, H, Shimamoto, K, Ehara, Y

    Mol. Gen. Genet.　216　188-194　1989/12

  133. Chemical composition and translation products of radish enation mosaic virus isolated in Japan Peer-reviewed

    Takahashi, H

    Ann. Phytopath. Soc. Japan　55　(2)　161-169　1989/12

    Publisher: The Phytopathological Society of Japan

    DOI： 10.3186/jjphytopath.55.161 　

    ISSN： 0031-9473

    More details Close

    Radish enation mosaic virus (RMV) is a member of the comovirus group, but a biochemical characterization of the group has been done only with cowpea mosaic virus (CpMV) which is the type member of the group. Therefore, the biochemical properties of other members of the group, such as RMV, are poorly understood. The present study was an attempt to analyze the nucleic acid, coat protein and gene composition of RMV. The purified RMV was fractionated into the three components (T, M and B components). B and M components were composed of two kinds of coat proteins with molecular weights of 47k and 18k daltons and contained single stranded RNAs with molecular weights of 2.0×106 and 1.6×106 daltons respectively, but T did not contain RNAs. The RNAs contained poly (A) sequences. Then, the B-RNA was in vitro translated into one polypeptide with a molecular weight of 200k daltons in the presence of zinc ions, which inhibited the protease activity. M-RNA was in vitro translated into two polypeptides with molecular weights of 115k and 105k daltons. These products in vitro translated from M and B-RNA were cleaved into eight kinds of polypeptides during incubation without the zinc ions. The results indicated that RMV might have essentially the same chemical composition and translation strategy as cowpea mosaic virus, except that the molecular weights of M-RNA and M-RNA translation products of RMV were quite different from those of CpMV.

  134. DNA sequence of gene VI of cauliflower mosaic virus Japanese strain S (CaMV S-Japan) Peer-reviewed

    H. Takahashi, K. Shimamoto, M. Suzuki, Y. Ehara

    Nucleic Acids Research　17　(19)　7981　1989/10/11

    DOI： 10.1093/nar/17.19.7981 　

    ISSN： 0305-1048

  135. Comparison of protein and mRNA syntheses in tobacco leaves infected with the yellow or the ordinary strain of cucumber mosaic virus. Peer-reviewed

    Takahashi, H, Ehara, Y

    Ann. Phytopath. Soc. Japan　54　(2)　164-173　1988/12

    Publisher: The Phytopathological Society of Japan

    DOI： 10.3186/jjphytopath.54.164 　

    ISSN： 0031-9473

    More details Close

    Infection of tobacco (Nicotiana tabacum L. cv. Ky57) leaves with the yellow strain of cucumber mosaic virus (CMV(Y)) resulted in large chlorotic spot formation, while no clear symptom appeared with the ordinary strain of the virus (CMV(O)) except for faint yellowing. In spite of the marked difference in symptom appearance on the tobacco between the two virus strains, the time course and extent of the virus titer and coat protein synthesis were essentially similar for both strains. In the leaves inoculated with CMV(O), 80 s ribosome concentration and host protein synthesis decreased temporarily, and then they were recovered moderately when the virus titer reached a maximum. 70 s ribosome concentration decreased to a small extent, and poly(A)-mRNA synthesis and polysome concentration were little affected throughout the experimental periods. In the leaves infected with CMV(Y), in comparison with CMV(O), poly(A)-mRNA synthesis and polysome concentration first decreased almost simultaneously, and followed by the decrease of host protein synthesis, and then 80 s and 70 s ribosome concentrations decreased significantly in the order. Thus, it is suggested that a series of such reactions lies at the basis of symptom appearance. Incidentally, no increase in activities of protease and ribonuclease was observed in the leaves inoculated with the two virus strains.

  136. ALTERED FRACTIONATION PROFILES OF HIGHER-PLANT POLY(A)-CONTAINING RNA BY SELECTIVE FORMATION OF A COMPLEX WITH A HYDROPHOBIC IMPURITY Peer-reviewed

    H TAKAHASHI, T NITTA

    PLANT SCIENCE　43　(1)　63-67　1986

    ISSN： 0168-9452

  137. SELECTIVE FORMATION OF A COMPLEX OF POLYADENYLATE WITH AN IMPURITY IN HIGHER-PLANT NUCLEIC-ACID PREPARATIONS Peer-reviewed

    T NITTA, H TAKAHASHI, K KOBAYASHI, K FUKUEI

    PLANT SCIENCE LETTERS　34　(1-2)　135-144　1984

    ISSN： 0304-4211

Show all ︎Show first 5

Misc. 212

  1. Activation of Plant Immune by Short Time Exposure of Plasma-generated N2O5 Gas

    岩本拡仁, 佐々木渉太, 高島圭介, 築舘大輝, 安藤杉尋, 高橋英樹, 東谷篤志, 豊田正嗣, 金子俊郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)　70th　2023

    ISSN： 2436-7613

  2. Growth analysis and influence on tomato fruit preservation in cultivated tomato plant irradiated with atmospheric pressure plasma.

    高橋英樹, 安藤杉尋, 宮下脩平, 高島圭介, 佐々木渉太, 金子俊郎

    日本植物病理学会報　89　(1)　2023

    ISSN： 0031-9473

  3. Activating plant immune system irradiated with novel reactive nitrogen species generated by atmospheric-pressure plasma device.

    築舘大輝, 高島圭介, 佐々木渉太, 宮下脩平, 金子俊郎, 高橋英樹, 安藤杉尋

    日本植物病理学会報　88　(1)　2022

    ISSN： 0031-9473

  4. Evaluation of plant stress due to plasma-generated reactive oxygen and nitrogen species using electrolyte leakage

    Keisuke Takashima, Ahmad Shahir bin Ahmad Nor, Sugihiro Ando, Hideki Takahashi, Toshiro Kaneko

    JAPANESE JOURNAL OF APPLIED PHYSICS　60　(1)　2021/01

    ISSN： 0021-4922

    eISSN： 1347-4065

  5. 水稲の有機栽培育苗土の微生物多様性と病害抑制効果 Invited

    安藤杉尋, 佐々木稜太, 高橋英樹

    土づくりとエコ農業　51　(552)　38-42　2019/09/20

    Publisher: 一般財団法人 日本土壌協会

  6. Suppression of rice seedling diseases by atmospheric plasma irradiation Invited Peer-reviewed

    Hideki Takahashi, Akihiro Ando, Keisuke Takashima, Toshiro Kaneko

    バイオサイエンスとインダストリー（Ｂ＆Ｉ）　76　(3)　2018/05/21

  7. 有機栽培培土の微生物コミュニティを利用したイネもみ枯細菌病の生物的防除

    安藤杉尋, 高橋英樹

    微生物農薬開発戦略のイノベーションと微生物群衆構造に着眼した生物的防除研究の動向 バイオコントロール研究会レポート　15　41-47　2018/03

  8. 菌に強い有機栽培育苗土の生物的特性について

    安藤杉尋, 高橋英樹

    有機農業者研究会議2018資料集　71-76　2018

  9. 有機農業を特徴づける客観的指標の開発と安定生産技術の開発 技術資料集

    安藤杉尋, 高橋英樹

    有機農業資料集　4-1-4-10　2018

  10. Analysis of the disease-suppressing effects of microorganisms included in nursery soils for organic farming of rice.

    Ando, S, Takahashi, H

    15　51-54　2018

  11. イネ有機育苗培土における微生物相のロバストネスと苗病害抑制現象

    高橋英樹, 松下裕子, 伊藤豊彰, 小林 隆, 橋本知義, 宮下脩平, 守川俊幸, 吉田重信, 對馬誠也, 安藤杉尋

    Soil Microorganisms　70　(3)　2016

  12. 昆虫病原菌を利用した生物防除における抵抗性誘導

    高橋英樹

    JATAFFジャーナル　4　(9)　21-23　2016

  13. Molecular basis of a bioinsecticide-activated plant defense system to suppress bacterial wilt disease caused by Ralstonia solanacearum

    Hideki Takahashi, Mitsuro Hyakumachi, Masafumi Shimizu, Yutaka Iwamoto, Masataka Aino, Katusnari Matsuura, Shinichi Goto, Kazuhiro Nakano, Shugihiro Ando, Tsutomu Arie, Seya Tsushima, Shigenobu Yoshida

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　248　2014/08

    ISSN： 0065-7727

  14. Recent studies on biological control of plant diseases in Japan

    Mitsuro Hyakumachi, Hideki Takahashi, Yoichi Matsubara, Nobutaka Someya, Masafumi Shimizu, Kappei Kobayashi, Masamichi Nishiguchi

    JOURNAL OF GENERAL PLANT PATHOLOGY　80　(4)　287-302　2014/07

    DOI： 10.1007/s10327-014-0524-4 　

    ISSN： 1345-2630

    eISSN： 1610-739X

  15. 有機農業を特徴づける客観的指標の開発と安定生産技術の開発

    安藤杉尋, 高橋英樹

    有機農業資料集　4-1-4-10　2014

  16. 植物免疫におけるプライミングとクロマチン修飾機構の解析

    安藤杉尋, Michal Jaskiewicz, 高橋英樹, Uwe Conrath

    植物感染生理談話会論文集「新視点から見渡す病原体感染戦略と植物免疫ネットワーク」　49　2014

    Publisher: 日本植物病理学会植物感染生理談話会

  17. PK-14 Suppression of crown and root rot disease of tomato by Bacillus and Paenibacillus isolates obtained from tomato phyllosphere(PK Interaction and symbiosis,Poster session)

    Sato Ikuo, Yoshida Shigenobu, Iwamoto Yutaka, Aino Masataka, Hyakumachi Mitsuro, Shimizu Masafumi, Takahashi Hideki, Ando Sugihiro, Tsushima Seiya

    (28)　173-173　2012

    Publisher: 日本微生物生態学会

  18. 日本産の抵抗性誘導微生物Pythium oligandrum菌株研究の現状. Peer-reviewed

    竹中重仁, 高橋英樹

    植物防疫　66　332-336　2012

  19. 日本発の抵抗性誘導微生物Pythium oligandrum研究の現状

    竹中重仁, 高橋英樹

    植物防疫　66　(6)　36-40　2012

    Publisher: 日本植物防疫協会

  20. キュウリモザイクウイルス防御応答におけるNB-LRRタンパク質の機能解析

    高橋英樹, 安藤杉尋

    植物感染生理談話会論文集「植物−病原微生物の相互作用研究の新展開」　47　135-144　2012

    Publisher: 日本植物病理学会植物感染生理談話会

  21. 有機栽培イネ細胞間隙に由来する微生物の解析と病害防除

    高橋英樹, 安藤杉尋

    有機農業研究者会議2012資料集　51-55　2012

  22. Analysis of endophytes from intercellular fluid of organically-grown rice plants and its effect on disease toleranc

    安藤杉尋, 高橋英樹

    Soil Microorganisms　65　(2)　100-103　2011

    Publisher: Japanese Society of Soil Microbiology

    DOI： 10.18946/jssm.65.2_100 　

    ISSN： 0912-2184

  23. モデル植物トマト：マイクロトムを用いた病害研究の現状と今後の展望

    高橋英樹, 長谷 修, 竹中重仁

    63　605-607　2010/09

  24. 非病原菌Pythium oligandrumを利用した広スペクトル微生物農薬の開発

    竹中重仁, 出崎里永子, 高橋英樹

    バイオインダストリー　27　(4)　39-46　2010/04

    Publisher: シーエムシー出版

    ISSN： 0910-6545

  25. Defense system induced by elicitin-like proteins of biocontrol agent Pythium oligandrum. Genome-enabled integration of research in plant-pathogen systems.

    Takahashi, H, Takenaka, S

    Genome-enabled integration of research in plant-pathogen systems. In 10th Japan-USA Scientific Seminar (Eds. T. Walpert, T. Shiraishim, K. Akimitsu and J. Glazebrook), APS Press, St Paul, Minnesota, USA　39-46　2010

  26. Recent Research Topics in the Laboratory of Plant Pathology

    IKEGAMI Masato, TAKAHASHI Hideki, ANDO Sugihiro

    Tohoku journal of agricultural research　60　(1)　45-50　2009/12

    Publisher: Graduate School of Agricultural Science, Tohoku University

    ISSN： 0040-8719

  27. 土壌生息菌Pythium oligandrumが生産するエリシチン様タンパク質により誘導される感染防御システムの分子機構

    高橋英樹, 竹中重仁

    34　339-341　2009/09

    DOI： 10.1584/jpestics.34.339 　

  28. 生物防除微生物Pythium oligandrum前接種トマト根部における青枯病菌の抑制の動態観察

    増中章, 中保一浩, 高橋英樹, 竹中重仁

    日本植物病理学会報　75　(3)　2009

    ISSN： 0031-9473

  29. Molecular mechanism for defense system induced by Elicitin-like proteins of nonpathogenic soil-inhibiting Pythium oligandrum

    Hideki Takahashi, Shigehito Takenaka

    JOURNAL OF PESTICIDE SCIENCE　34　(4)　339-341　2009

    ISSN： 1348-589X

  30. Primary biocontrol mechanism of tomato bacterial wilt by Pythium oligandrum.

    Takenaka S, Yamaguchi K, Inoue T, Hase S, Masunaka A, Sekiguchi H, Takahashi H

    47-49　2009

  31. Spermine signaling in defense reaction against avirulent viral pathogen in Arabidopsis thaliana

    G. H.M. Sagor, Run-Zi Cong, Thomas Berberich, Hideki Takahashi, Yoshihiro Takahashi, Tomonobu Kusano

    Plant Signaling and Behavior　4　(4)　316-318　2009

    Publisher: Landes Bioscience

    DOI： 10.4161/psb.4.4.8104 　

    ISSN： 1559-2324 1559-2316

  32. Study on interaction between Cucumber mosaic virus and host plants at a molecular level

    Hideki Takahashi

    JOURNAL OF GENERAL PLANT PATHOLOGY　74　(6)　454-456　2008/12

    DOI： 10.1007/s10327-008-0129-x 　

    ISSN： 1345-2630

  33. (397) Analysis of Sites Responsible for Inducing Tomato Plant Defense in Cell Wall Proteins Isolated from Pythium spp(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Takenaka S., Masunaka A., Sekiguchi H., Hase S., Takahashi H.

    Annals of the Phytopathological Society of Japan　74　(3)　273-273　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  34. (239) Strength of Resistance to Cucumber mosaic virus Depends on Amount of Resistance Gene Products, RCY1(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Sekine K.-T., Kawakami S., Hase S., Takahashi H.

    Annals of the Phytopathological Society of Japan　74　(3)　230-230　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  35. (240) Analysis of the Activation of Salicylic Acid Dependent Signaling Pathway in the Resistance to Cucumber mosaic virus using GFP Reporter System(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Kai A., Sekine K. T., Kawamura Y., Hase S., Yamashita M., Tomita H., Takahashi H.

    Annals of the Phytopathological Society of Japan　74　(3)　230-231　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  36. (395) Involvement of ATL 16, Ubiquitin Ligase, in Defense Response in Arabidopsis thaliana(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Kubota M., Kawamura Y., Sekine K.T., Hase S., Takahashi H., Hyakumachi M.

    Annals of the Phytopathological Society of Japan　74　(3)　272-272　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  37. (398) Role of Cyanoalanine on Disease Resistance Induced by Pythium oligandrum(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Takahashi S., Hase S., Kawamura Y., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　74　(3)　273-273　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  38. (399) Involvement of SGR1, RAR1 and NPR1 in Induced Resistance by Pythium oligandrum(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Hase S., Kawamura Y., Kubota M., Ichinose Y., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　74　(3)　273-273　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  39. (424) Isolation of DNA from Microorganism in Rice Intercellular Fluid and Analysis of PCR-amplified 18SrDNA(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Yamauchi Y., Kawakami S., Sekine K.-T., Hase S., Itou T., Hatanaka N., Sasahara T., Sekiguchi H., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　74　(3)　280-280　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  40. (294) Virus-induced Gene Silencing of RCY1 Gene in Arabidopsis thaliana using Apple latent spherical virus(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Tamura A., Sugai T., Isogai M., Takahashi H., Yoshikawa N.

    Annals of the Phytopathological Society of Japan　74　(3)　245-245　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  41. (401) Induction Mechanism for the Necrosis on Arabidopsis thaliana by Turnip mosaic virus Infection(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Inukai T, Kim B, Masuta C, Matsuura H, Takahashi H

    Annals of the Phytopathological Society of Japan　74　(3)　274-274　2008/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  42. キュウリモザイクウイルスー宿主植物の相互作用に関する分子生理学的研究

    高橋英樹

    日本植物病理学会報　74　(3)　134-136　2008/08

    Publisher: The Phytopathological Society of Japan (PSJ)

    DOI： 10.3186/jjphytopath.74.134 　

    ISSN： 0031-9473

  43. (8)Conforcal Laser Scanning Microscopic Analysis of Tomato Root Colonization by Biocontrol Agent Pythium oligandrum and Ralstonia solanacearum(Abstracts Presented at the Meeting of the Hokkaido Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Masunaka A., Nakaho K., Takahashi H., Takenaka S.

    Annals of the Phytopathological Society of Japan　74　(1)　80-81　2008/02/20

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  44. (9)Induced Resistance in Sugar Beet Leaves by Treatment with Cell Wall Proteins from the Colonization of the Biocontrol Agent Pythium oligandrum(Abstracts Presented at the Meeting of the Hokkaido Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　74　(1)　81-81　2008/02/20

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  45. (2)Arabidopsis Transformed with GUS Reporter Construct under the Control of Plant-activators- and Othylene-inducible Cyanoalanine Synthase Promoter(Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Sekine K.-T., Takahashi S., Kai A., Hase S., Yamashita M., Tomita H., Takahashi H.

    Annals of the Phytopathological Society of Japan　74　(1)　48-48　2008/02/20

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  46. (1)Study on Molecular Mechanism of Resistance Induced by Pythium oligandrum : Activation of Jasmonic Acid Signaling Pathway in Cyanoalanine-Treated Tomato Plants Pythium oligandrum (PO)(Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Takahashi S., Hase S., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　74　(1)　48-48　2008/02/20

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  47. (3)Global Gene Expression Analysis and Activation of Ethylene-Mediated Signaling Pathways on INF1-Treated Tomato Leaves(Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Kawamura Y., Hase S., Yoshioka H., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　74　(1)　48-49　2008/02/20

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  48. (11)Isolation of DNA from Microorganisms in Rice Intercellular Fluid and Analysis of PCR-amplified 16SrDNA(Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Takahashi H., Sekiguchi H., Ito T., Yamauchi Y., Kawakami S., Sekine K.-T., Hase S., Takenaka H.

    Annals of the Phytopathological Society of Japan　74　(1)　50-51　2008/02/20

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  49. マイクロトムトマトのゲノム情報を活用したPythium oligandrumによる誘導抵抗性の分子機構解明

    長谷 修, 竹中重仁, 高橋英樹

    植物感染生理談話会論文集　44　49-58　2008

  50. モデル植物を用いた機構解析-トマト（マイクロトム）

    竹中重仁, 高橋英樹

    微生物と植物の相互作用を利用した病害防除（百町満郎・對馬誠也 監修）ソフトサイエンス社　196-203　2008

  51. (290) Analysis of Defense Gene Expression on INF1-Treated Tomato Leaves(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Kawamura Y., Hase S., Yoshioka H., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(3)　252-253　2007/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  52. (293) Identification of a Protein Interacting with LeATL6 Ubiquitin Ligase Induced by Fungal Glycoprotein Elicitor(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Takahashi H., Hondo D., Hase S., Takenaka S.

    Annals of the Phytopathological Society of Japan　73　(3)　253-253　2007/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  53. (291) Analysis of Peptides Carrying an Elicitor Activity in a Protein Elicitor, POD-1, of the Biocontrol Agent Pythium oligandrum(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(3)　253-253　2007/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  54. (292) Global Analysis of Gene Expression in Pythium oligandrum-treated Tomato Plants after Challenge Inoculation with Ralstonia solanacearum(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Hase S., Ishihara T., Nakaho K., Tsugane T., Shibata D., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(3)　253-253　2007/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  55. (220) Response to Cucumber mosaic virus Infection on Arabidopsis thaliana Overexpressing WRKY33(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Osanai T., Sekine K.-T., Ishihara T., Kawakami S., Hase S., Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(3)　233-234　2007/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  56. (219) Highlevel Expression of a Resistance Gene, RCY1 Induce Extreme Resistance to Cucumber mosaic virus(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Sekine K.-T., Ishihara T., Kawakami S., Hase S., Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(3)　233-233　2007/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  57. (221) Silencing of ACD2 in Tomato Infected with Cucumber mosaic virus Containing ACD2 Sequence(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Ishihara T., Takahashi H., Masuta C.

    Annals of the Phytopathological Society of Japan　73　(3)　234-234　2007/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  58. Plant defense strategies against viruses

    Chikara Masuta, Hideki Takahashi

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　52　(10)　1221-1226　2007/08/01

    Publisher: 共立出版

    ISSN： 0039-9450

  59. 植物のウイルスに対する防御機構

    増田 税, 高橋英樹

    蛋白質核酸酵素、ウイルス研究の現在と展望　52　(10)　1221-1226　2007/07

    Publisher: 共立出版

  60. Identification of a novel Cys(2)/His(2)-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco

    Yoshiko Mitsuya, Yoshihiro Takahashi, Yukiko Uehara, Thomas Berberich, Atsushi Miyazaki, Hideki Takahashi, Tomonobu Kusano

    JOURNAL OF PLANT PHYSIOLOGY　164　(6)　785-793　2007/06

    DOI： 10.1016/j.jplph.2006.05.011 　

    ISSN： 0176-1617

  61. Colonization of the Biocontrol Agent Pythium oligandrum in Tomato Rhizosphere Analyzed by Real-Time PCR and Confocal Laser Scanning Microscopy(Abstracts Presented at the Meeting of the Hokkaido Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Takenaka S., Sekiguchi H., Masunaka A., Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(1)　78-78　2007/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  62. Arabidopsis thaliana Ecotype Columbia Transformed with the RCY1 Gene Induce Resistance to Cucumber mosaic virus(Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Sekine K.-T., Ishihara T., Kawakami S., Hase S., Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(1)　46-46　2007/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  63. Analysis of Genomic RNA Sequence of Radish mosaic virus and its Distribution in Tohoku(Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Hase S., Hoshi A., Sekine K.-T., Satoh K., Hondo D., Sano T., Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(1)　47-47　2007/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  64. EDS5-overexpressing Arabidopsis thaliana Expresses Salicylic Acid-dependent and -independent Basal Resistance to Virulent Viruses(Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Ishihara T., Sekine K.-T., Hase S., Shah Jyoti, Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(1)　46-47　2007/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  65. Analysis of Jasmonate Signaling Pathway Activated by Pythium oligandrum-inducible LeATL6 in Tomato(Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Hondo D., Hase S., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(1)　51-51　2007/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  66. Structural Analysis of RPP8/HRT/RCY1 Locus in Arabidopsis thaliana Ecotypes Er-0, Sf-2 and Var2-1(Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Kawakami S., Sekine K.-T., Ishihara T., Hase S., Takahashi H.

    Annals of the Phytopathological Society of Japan　73　(1)　46-46　2007/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  67. Veinal Necrosis Induced by Turnip mosaic virus Infection in Arabidopsis thaliana is HR-like Cell Death(Abstracts Presented at the Meeting of the Hokkaido Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Kim B., Inukai T., Takahashi H., Masuta C.

    Annals of the Phytopathological Society of Japan　73　(1)　78-79　2007/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  68. The LeATL6-associated ubiquitin/proteasome system may contribute to fungal elicitor-activated defense response via the jasmonic acid-dependent signaling pathway in tomato

    Daisuke Hondo, Shu Hase, Yoshinori Kanayama, Nobuyuki Yoshikawa, Shigehito Takenaka, Hideki Takahashi

    MOLECULAR PLANT-MICROBE INTERACTIONS　20　(1)　72-81　2007/01

    DOI： 10.1094/MPMI-20-0072 　

    ISSN： 0894-0282

  69. Tomato as a model plant for plant-pathogen interactions

    Tsutomu Arie, Hideki Takahashi, Motoichiro Kodama, Tohru Teraoka

    Plant Biotechnology　24　135-157　2007/01/01

    DOI： 10.5511/plantbiotechnology.24.135 　

    ISSN： 1342-4580

  70. プラントアクティベーター開発につながる生物防除微生物による誘導抵抗性機構

    竹中重仁, 高橋英樹

    植物防疫　61　(10)　2007

  71. (246) Analysis of Mechanism of Reducing the Severity of Bacterial Wilt Disease in Tomato by the Biocontrol Agent Pythium oligandrum Using a POD-1, a Elicitor Protein Gene, Deficient Isolate(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Takenaka S., Masunaka A., Kono T, Sekiguchi H., Shimizu A., Hase S., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(4)　267-267　2006/11/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  72. (340) Single Amino Acid Substitutions on Cucumber mosaic virus Resistance Protein RCY1 Compromise Resistance to Virus but Differentially Suppress Hypersensitive Cell Death in Arabidopsis(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Sekine K.-T., Ishihara T., Hase S., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(4)　292-293　2006/11/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  73. (339) Activation of Salicylic Acid-Mediated Signaling Pathway and Resistance to Viruses in EDS5-Overexpressing Arabidopsis thaliana(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Ishihara T., Sekine K.-T., Hase S., Shah J., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(4)　292-292　2006/11/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  74. (341) Diversity at RPP8/HRT/RCY1 Locus Encoding CC-NBS-LRR Class R Protein in Arabidopsis thaliana(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Kawakami S., Sekine K.-T., Ishihara T., Hase S., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(4)　293-293　2006/11/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  75. (248) Jasmonate-mediated Signaling Pathway Is Involved in Pythium oligandrum-induced Resistance to Ralstonia solanacearum in Tomato Plants(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Hase S., Nakaho K., Arie T., Seo S., Ohashi Y., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(4)　267-268　2006/11/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  76. (249) Analysis of Altered Gene Expression in Tomato, Rice and Potato Treated with Oospore Suspension or Cell Wall Protein Elicitor of Pythium oligandrum(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Shimizu A., Hase S., Ishihara T., Shibata D., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(4)　268-268　2006/11/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  77. (251) Beta-cyanoalanine Synthase as a Molecular Marker for Induced Resistance by Fungal Glycoprotein Elicitor and Commercial Plant Activators(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Takahashi H., Ishihara T., Hase S., Nakaho K., Arie T., Teraoka T., Iwata M., Tugane T., Shibata D., Takenaka S.

    Annals of the Phytopathological Society of Japan　72　(4)　268-269　2006/11/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  78. (250) Function of RING-H2 Zinc-finger Protein Induced in Tomato Treated with the Oospore Suspension of Pythium oligandrum(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Hondo D., Hase S., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(4)　268-268　2006/11/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  79. Beta-cyanoalanine synthase as a molecular marker for induced resistance by fungal glycoprotein elicitor and commercial plant activators

    Hideki Takahashi, Takeaki Ishihara, Shu Hase, Ayaka Chiba, Kazuhiro Nakaho, Tsutomu Arie, Tohru Teraoka, Michiaki Iwata, Taneaki Tugane, Daisuke Shibata, Shigehito Takenaka

    PHYTOPATHOLOGY　96　(8)　908-916　2006/08

    DOI： 10.1094/PHYTO-96-0908 　

    ISSN： 0031-949X

    eISSN： 1943-7684

  80. The natural occurrence of two distinct begomoviruses associated with DNA beta and a recombinant DNA in a tomato plant from Indonesia

    T Kon, SH Hidayat, S Hase, H Takahashi, M Ikegami

    PHYTOPATHOLOGY　96　(5)　517-525　2006/05

    DOI： 10.1094/PHYTO-96-0517 　

    ISSN： 0031-949X

  81. Quantitative Analysis of Populations of the Biocontrol Agent Pythium oligandrum Isolates in the Rhizosphere of Tomato Using Real-Time PCR (Abstracts Presented at the Meeting of the Hokkaido Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Takenaka S., Sekiguchi H., Masunaka A., Kohno T., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(1)　84-84　2006/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  82. Analysis of the Resistance Gene to Cucumber mosaic virus, RCY1 in Arabidopsis : Isolation of rcy1 Mutants and Complementation Test of RCY1 function in the rcy1 Mutant (Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Sekine K. -T., Ishihara T., Hase S., Ikegami M., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(1)　57-57　2006/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  83. Elevated Level of Gene Expression for Shikimate Pathway in Tomato Treated with Elicitin-like Protein Elicitor of Pythium oligandrum Pythium oligandrum (Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Takahashi H., Ishihara T., Chiba A., Hase S., Takenaka S.

    Annals of the Phytopathological Society of Japan　72　(1)　58-58　2006/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  84. Analysis of Enhanced Resistant Response to Cucumber mosaic virus in EDS5-Overexpressing Arabidopsis thaliana (Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Ishihara T., Sekine K. -T., Hase S., Ikegami M., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(1)　57-58　2006/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  85. Analysis of Ethylenemediated Signaling Pathway on Pythium oligandrum-induced Resistance to Ralstonia solanacearum in Tomato Plants (Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Hase S., Shimizu A., Nakaho K., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　72　(1)　58-58　2006/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  86. Screening of Host Factors that Interact with Replication Associated-Protein (Rep) of Tomato leaf curl Java virus (Abstracts Presented at the Meeting of the Tohoku Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Nonaka A., Tokita A., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　72　(1)　57-57　2006/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  87. Analysis of spermine-signaling pathway in Arabidopsis thaliana

    Y Mitsuya, Y Takahashi, T Berberich, A Miyazaki, H Matsumura, H Takahashi, R Terauchi, T Kusano

    PLANT AND CELL PHYSIOLOGY　47　S244-S244　2006

    ISSN： 0032-0781

  88. Begomoviruses associated with leaf curl disease of tomato in Java, Indonesia

    Sukamto, T Kon, SH Hidayat, K Ito, S Hase, H Takahashi, M Ikegami

    JOURNAL OF PHYTOPATHOLOGY　153　(9)　562-566　2005/09

    DOI： 10.1111/j.1439-0434.2005.01020.x 　

    ISSN： 0931-1785

  89. (1) Catalog of Micro-Tom Tomato Responses to Common Fungal, Bacterial and Viral Pathogens(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Watanabe Y., Hori K., Arie T., Rosmalawati S., Fukushima S., Kikuchi M., Hikichi Y., Kanda A., Takahashi A., Kiba A., Ohnishi K., Ichinose Y., Taguchi F., Yasuda C., Kodama M., Egusa M., Masuta C., Sawada H., Shibata D., Takahashi H., Shimizu A.

    Annals of the Phytopathological Society of Japan　71　(3)　185-185　2005/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  90. (283) Analysis of the Satellite DNA Molecule of Tomato leaf curl Java virus (III) : The βC1 Protein Suppresses Gene Silencing and Induces Symptom(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Kon T., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　71　(3)　261-261　2005/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  91. (324) Analysis of a Novel Gene Expressed at the Early Stage of CMV(Y) Resistance Response in Arabidopsis thaliana(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Ishihara T., Hase S., Ikegami M., Takahashi H.

    Annals of the Phytopathological Society of Japan　71　(3)　272-272　2005/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  92. (321) Screening of Host Factors that Interact with Replication Associated-Protein (Rep) of Tomato leaf curl Java virus using Yeast Two-hybrid Analysis(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Tokita A., Nonaka A., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　71　(3)　271-271　2005/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  93. (345) The Screening of Arabidopsis Mutants Being Susceptible to Cucumber mosaic virus and the Characterization of Three Mutants; rcy1-2, rcy1-3 and rcy1-4(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Sekine K. T., Hase S., Ikegami M., Takahashi H.

    Annals of the Phytopathological Society of Japan　71　(3)　277-278　2005/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  94. (369) Study on the Colonization Ability of the Biocontrol Agent Pythium oligandrum (PO) in Rhizosphere (1) : Quantitative Assay of PO Populations in the Rhizosphere of "Micro-Tom" Tomato(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Takenaka S., Sekiguchi H., Takahashi H., Nakaho K.

    Annals of the Phytopathological Society of Japan　71　(3)　284-284　2005/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  95. (371) Study on the Molecular Mechanism of the Resistance Induced by the Biocontrol Agent Pythium oligandrum and Its Cell Wall Proteinaceous Elicitor (1) : Role of Ethylene-mediated Signaling Pathway on Induced Resistance in "Micro-Tom" Tomato(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Hase S., Shimizu A., Takenaka S., Takahashi H.

    Annals of the Phytopathological Society of Japan　71　(3)　284-285　2005/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  96. (372) Study on the Molecular Mechanism of the Resistance Induced by the Biocontrol Agent Pythium oligandrum (PO) and Its Cell Wall Proteinaceous Elicitor (2) : Global Analysis of Gene Expression in "Micro-Tom" Tomato Root Treated with PO-derived Cell Wall Proteinaceous Elicitor(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Takahashi H., Hase S., Ishihara T., Nakaho K., Shibata D., Takenaka S.

    Annals of the Phytopathological Society of Japan　71　(3)　285-285　2005/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  97. (9) Genetic Diversity of Tomato and Ageratum-infecting Begomoviruses from Indonesia(Abstracts Presented at the Meeting of the Tohoku Division, Fukushima, October 5-6, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Sukamto, Kon T., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　71　(1)　46-46　2005/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  98. (10) Evidence for Natural Recombination between Tomato leaf curl Java virus and Ageratum yellow vein virus-[Tomato](Abstracts Presented at the Meeting of the Tohoku Division, Fukushima, October 5-6, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Kon T., Kuwabara K., Hase S., Takahashi H., Hidayat S. H., Ikegami M.

    Annals of the Phytopathological Society of Japan　71　(1)　46-46　2005/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  99. Catalog of Micro-Tom tomato responses to common fungal, bacterial, and viral pathogens

    Hideki Takahashi, Ayano Shimizu, Tsutomu Arie, Syofi Rosmalawati, Sumire Fukushima, Mari Kikuchi, Yasufumi Hikichi, Ayami Kanda, Akiko Takahashi, Akinori Kiba, Kohei Ohnishi, Yuki Ichinose, Fumiko Taguchi, Chihiro Yasuda, Motoichiro Kodama, Mayumi Egusa, Chikara Masuta, Hiroyuki Sawada, Daisuke Shibata, Koichi Hori, Yuichiro Watanabe

    Journal of General Plant Pathology　71　(1)　8-22　2005/02

    DOI： 10.1007/s10327-004-0168-x 　

    ISSN： 1345-2630

  100. Identification of the cis-acting elements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment

    MS Zheng, H Takahashi, A Miyazaki, K Yamaguchi, T Kusano

    PLANT SCIENCE　168　(2)　415-422　2005/02

    DOI： 10.1016/j.plantsci.2004.09.016 　

    ISSN： 0168-9452

  101. (257) Analysis of Two Satellite DNA Molecules of Tomato leaf curl Indonesia virus Which Differ in Pathologenicity (II)(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Kon T., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　70　(3)　259-260　2004/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  102. (301) Analysis of Gene Expression of NBS-LRR Gene Family Members in Cucumber mosaic virus-infected Arabidopsis thaliana(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Takahashi H., Ishihara T., Sakurai N., Kawakami S., Sekine K-T., Hase S., Ikegami M., Shah Jyoti, Sibata D.

    Annals of the Phytopathological Society of Japan　70　(3)　271-272　2004/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  103. (258) Analysis of Nicotiana benthamiana to Which the C4 Gene of Tomato leaf curl Indonesia virus Was Introduced Tomato leaf curl virus(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Sakai Y., Hase S., Takahasi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　70　(3)　260-260　2004/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  104. (256) Ageratum yellow vein virus and Satellite DNA Isolated from Tomato Showing Leaf Curl Symptoms in Indonesia(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Kon T., Sukamto Sukamto, Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　70　(3)　259-259　2004/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  105. (300) Expression Analysis of a CMV-inducible Tobacco Gene in Response to Abiotic Stresses(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　70　(3)　271-271　2004/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  106. ウイルス感染に対する宿主応答

    高橋英樹

    平成16年度植物感染生理談話会論文集ー自他識別と応答のバイオフロンティアー　2004/08

    Publisher: 日本植物病理学会

  107. Up-regulation of Arabidopsis thaliana NHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signalling pathways that differ in salicylate involvement

    MS Zheng, H Takahashi, A Miyazaki, H Hamamoto, J Shah, Yamaguchi, I, T Kusano

    PLANTA　218　(5)　740-750　2004/03

    DOI： 10.1007/s00425-003-1169-2 　

    ISSN： 0032-0935

  108. (29) The Response to Cucumber mosaic virus in Arabidopsis Mutants Induced Systemic Acquired Resistance (Abstracts Presented at the Meeting of the Tohoku Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Sekine K. T., Nandi Ashis, Hase S., Ikegami M., Shah Jyoti, Takahashi H.

    Annals of the Phytopathological Society of Japan　70　(1)　55-55　2004/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  109. (17) Analysis of Two leaf curl Indonesia virus Satellite DNA Molecules that Differ in Pathogenicity (Abstracts Presented at the Meeting of the Tohoku Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Kon T., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　70　(1)　52-52　2004/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  110. (27) Analysis of Altered Gene Expressions in Arabidopsis thaliana during the Early Stage of Cucumber mosaic virus Infection (Abstracts Presented at the Meeting of the Tohoku Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Ishihara T., Hase S., Ikegami M., Shah Jyoti, Takahashi H.

    Annals of the Phytopathological Society of Japan　70　(1)　55-55　2004/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  111. (28) Analysis of Signaling Pathway for the Resistance to Cucumber mosaic virus (VI) : Involvement of PAD4/EDS1 in the Resistance of CMV (Abstracts Presented at the Meeting of the Tohoku Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Takahashi H., Hase S., Ikegami M.

    Annals of the Phytopathological Society of Japan　70　(1)　55-55　2004/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  112. (15) Intracellular Localization of Tomato leaf curl Indonesia virsu Proteins Associated with Viral Movement (Abstracts Presented at the Meeting of the Tohoku Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Hatanaka N., Kon T., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　70　(1)　51-52　2004/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  113. (16)Infectious Clone of Tomato leaf curl Bandung virus.(Abstracts Presented at the Meeting of the Tohoku Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Sukamto Sukamto, Kon T., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　70　(1)　52-52　2004/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  114. Enhanced resistance to Cucumber mosaic virus in the Arabidopsis thaliana ssi2 mutant is mediated via an SA-independent mechanism

    Ken-Taro Sekine, Ashis Nandi, Takeaki Ishihara, Shu Hase, Masato Ikegami, Jyoti Shah, Hideki Takahashi

    Molecular Plant-Microbe Interactions　17　(6)　623-632　2004

    Publisher: American Phytopathological Society

    DOI： 10.1094/MPMI.2004.17.6.623 　

    ISSN： 0894-0282

  115. Molecular characterization of a strain of Squash leaf curl China virus from the Philippines

    T Kon, LM Dolores, NB Bajet, S Hase, H Takahashi, M Ikegami

    JOURNAL OF PHYTOPATHOLOGY　151　(10)　535-539　2003/10

    DOI： 10.1046/j.1439-0434.2003.00764.x 　

    ISSN： 0931-1785

  116. (345)Evidence for Natural Recombination between Genomic and Satellite DNAs of Tomato leaf curl Indonesia virus(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Sukamto Sukamto, Kon T., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　69　(3)　325-326　2003/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  117. Study on the Signal Transduction Pathway for the Resistance Response to Cucumber mosaic virus. (V) Resistance to CMV in eds5/ein2/coi1 Triple Mntant of Arabidopsis thaliana(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Takahashi H., Sekine K., Hase O., Ikegami M.

    Annals of the Phytopathological Society of Japan　69　(3)　332-333　2003/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  118. Tomato leaf curl Indonesia virus Differs in Symptom Development and Viral Accumulation in Natural Infected and Agoinoculated Tomato Plants(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Kon T., Sukamto Sukamto, Hase M., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　69　(3)　326-326　2003/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  119. Global Changes in Gene Expression in Resistance Response to Cucumber mosaic virus in Arabidpsis thaliana(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Ishihara T., Sakurai N., Hase O., Ikegami M., Shibata D., Takahashi H.

    Annals of the Phytopathological Society of Japan　69　(3)　333-333　2003/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  120. Construction of an Infectious cDNA Clone of Patchouli mild mosaic virus RNA2(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    kaizawa K., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　69　(3)　329-329　2003/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  121. Expression Analysis of Defense-related Genes in Sugar Beet and Arabidopsis Treated with a Cell Wall Protein Fraction from Pythium oligandrum Pythium oligandrum(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Takenaka S., Takenaka H., Nakamura Y.

    Annals of the Phytopathological Society of Japan　69　(3)　253-253　2003/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  122. Activation of defense-related gene expression and systemic acquired resistance in cucumber mosaic virus-infected tobacco plants expressing the mammalian 2 ' 5 ' oligoadenylate system - Brief report

    A Honda, H Takahashi, T Toguri, T Ogawa, S Hase, M Ikegami, Y Ehara

    ARCHIVES OF VIROLOGY　148　(5)　1017-1026　2003/05

    DOI： 10.1007/s00705-002-0959-3 　

    ISSN： 0304-8608

  123. Detection of Satellite DNA from Tomato leaf curl virus-Indonesia infected Tomato

    Sukamto Sukamto, Kon T., Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　69　(1)　35-35　2003/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

    More details Close

    Tomato Satellite DNA of Tomato leaf curl virus-Indonesia (ToLCV sat-DNA) was detected from natural infected tomato plants. ToLCV sat-DNA was approximately half-length of genomic DNA of ToLCV (1347 b). ToLCV sat-DNA was low sequence identity to the genomic DNA (41%). ToLCV sat-DNA contained 5 ORFs (V1-5) in virion-sense and 4 ORFs (C1-4) in complementary-sense each capable of encoding proteins with a molecular weight greater than 4 kDa. In intergenic region, a 34-base potential stem-loop forming region was found. It included the conserved nonanucleotide sequence TAATATTAC. The repetitive sequence GCTACGCA and GCTACGCC were found in intergenic region. The sequence of ToLCV sat-DNA contained an A-rich region between nucleotides 708 and 1002 (60% A). ToLCV sat-DNA had 80% and 63% nucleotide sequence identity to AYVD DNAβ Singapore and CLCuD DNAβ, respectively.

  124. Involvement of EDS5 in Resistance Response to Cucumber mosaic virus in Arabidpsis

    Ishihara T., Takeda M., Sekine K., Hase S., Ikegami M., Takahashi H.

    Annals of the Phytopathological Society of Japan　69　(1)　32-33　2003/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  125. Analysis of the Signal Transduction Pathway (s) for the Resistance to Cucumber mosaic virus Induced in Arabidopsis ssi2-1 Mutant

    Sekine K., Nandi Ashis, Shah Jyoti, Ishihara T., Hase S., Ikegami M., Takahashi H.

    Annals of the Phytopathological Society of Japan　69　(1)　33-33　2003/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  126. Role of SNF1-like Protein Kinase Specifically Binding to the Coat Protein of Cucumber mosaic virus for Hypersensitive Response on Tobacco

    Annals of the Phytopathological Society of Japan　69　(1)　33-33　2003/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  127. Genome Organization and Construction of an Infectious Clone of Tomato leaf curl virus-Indonesia

    Kon T., Sukamto Sukamto, Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　69　(1)　34-35　2003/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  128. Pseudorecombinant between Patchouli mild mosaic virus and Broad bean wilt virus 2-Miyagi Isolate

    Kaizawa K., Hase S., Takahashi H., Ikegami M., Nakamura S., Kuroda T., Suzuki K.

    Annals of the Phytopathological Society of Japan　69　(1)　32-32　2003/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  129. Differential Display Screening of Tobacco Genes Involved in the Development of Green Areas of Cucumber mosaic virus-infected Leaves

    Hase S., Takahashi H., Ikegami M.

    Annals of the Phytopathological Society of Japan　69　(1)　33-34　2003/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  130. Genome organization of an infectious clone of Tomato leaf curl virus (Philippines), a new monopartite Begomovirus

    T Kon, LM Dolores, A Murayama, NB Bajet, S Hase, H Takahashi, M Ikegami

    JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT　150　(11-12)　587-591　2002/12

    DOI： 10.1046/j.1439-0434.2002.00817.x 　

    ISSN： 0931-1785

  131. RCY1, an Arabidopsis thaliana RPP8/HRT family resistance gene, conferring resistance to cucumber mosaic virus requires salicylic acid, ethylene and a novel signal transduction mechanism

    H Takahashi, J Miller, Y Nozaki, Sukamto, M Takeda, J Shah, S Hase, M Ikegami, Y Ehara, SP Dinesh-Kumar

    PLANT JOURNAL　32　(5)　655-667　2002/12

    DOI： 10.1046/j.1365-313X.2002.01453.x 　

    ISSN： 0960-7412

  132. Ultrastructure of necrotic lesions in Nicotiana glutinosa leaves locally infected with a variant of Cucumber mosaic virus

    T Ishihara, H Takahashi, S Hase, T Sato, M Ikegami, Y Ehara

    JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT　150　(10)　553-556　2002/10

    DOI： 10.1046/j.1439-0434.2002.00802.x 　

    ISSN： 0931-1785

  133. (204)Analysis of the Resistance to Cucumber mosaic virus in Arabidopsis ssi2-1 Mutant

    Sekine K., Shah J., Hase S., Takahashi H.

    Annals of the Phytopathological Society of Japan　68　(2)　210-211　2002/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  134. (205)Role of SNF1-like Protein Kinase Specifically Binding to the Coat Protein of Cucumber mosaic virus for Symptom Expression on Tobacco

    Uchiyama T., Yoshioka K., Matsushita Y., Nyunoya H., Takahashi H., Hase S., Ehara Y.

    Annals of the Phytopathological Society of Japan　68　(2)　211-211　2002/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  135. (203)Study on the Signal Transduction Pathway in the Resistance Response to Cucumber mosaic virus. (V) Isolation of CMV Resistance gene, RCY1, from Arabidopsis thaliana

    Takahashi H., Nozaki Y., Sukamuto, Hase S., Ehara Y.

    Annals of the Phytopathological Society of Japan　68　(2)　210-210　2002/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  136. Phosphorylation of the movement protein of Cucumber mosaic virus in transgenic tobacco plants

    Y Matsushita, K Yoshioka, T Shigyo, H Takahashi, H Nyunoya

    VIRUS GENES　24　(3)　231-234　2002/06

    DOI： 10.1023/A:1015324415110 　

    ISSN： 0920-8569

  137. Characterization of a Cucumber mosaic virus Mutants which was Isolated from the Symptomless Tobacco Plants regenerated in vitro from CMV-Infected Tobacco Leaves by the Tissue Culture(Abstracts Presented at the Meeting of the Tohoku Division)

    Ito M., Takahashi H., Hase S., Ehara Y.

    Annals of the Phytopathological Society of Japan　68　(1)　56-56　2002/04/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  138. Study on the Signal Transduction Pathway in the Resistance Response to Cucumber mosaic virus. (IV) Role of Salicylic acid- and Ethylene-mediated Signal Transduction Pathway(Abstracts Presented at the Meeting of the Tohoku Division)

    Takahashi H., Takeda M., Hase S., Ehara Y.

    Annals of the Phytopathological Society of Japan　68　(1)　56-57　2002/04/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  139. Ultrastructural Observation of Cucumber mosaic virus-Infected Tobacco Tissues Showing the Hypersensitive Resistance Response(Abstracts Presented at the Meeting of the Tohoku Division)

    Ishihara T., Sato T., Hase S., Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　68　(1)　56-56　2002/04/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  140. Analysis of the Defense-Related Gene Expression in Cucumber mosaic virus - Infected Tobaccos Expressing the Mammalian 2',5' Oligoadenylate System(Abstracts Presented at the Meeting of the Tohoku Division)

    Honda A., Takahashi H., Toguri H., Ogawa T., Hase S., Ehara Y.

    Annals of the Phytopathological Society of Japan　68　(1)　57-57　2002/04/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  141. キュウリモザイクウイルスーシロイヌナズナ系におけるウイルス抵抗性反応

    高橋英樹, 野崎雪音, スカムト, 武田 愛, 長谷 修, 江原淑夫

    植物ウイルス病研究会レポート　8　69-77　2002/04

  142. Molecular approach to the study of sex determination in cucucmber plants

    S. Yamasaki, N. Fujii, H. Takahashi, H. Mizusawa, S. Matsuura

    Acta Horticulturae　588　309-312　2002/01/01

    DOI： 10.17660/ActaHortic.2002.588.49 　

    ISSN： 0567-7572

  143. The genome organization of pea stem necrosis virus and its assignment to the genus Carmovirus

    Shiho Suzuki, Shu Hase, Hideki Takahashi, Masato Ikegami

    Intervirology　45　(3)　160-163　2002

    DOI： 10.1159/000065870 　

    ISSN： 0300-5526

  144. Fine Mapping of RCY1, a Resistance Gene to Cucumber mosaic virus, on Arabidopsis thaliana

    Takahashi H., Nozaki Y., Sato D., Ehara Y.

    Annals of the Phytopathological Society of Japan　67　(2)　149-149　2001/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  145. Study on the Signal Transduction Pathway in the Resistance Response to Cucumber mosaic virus (III)

    Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　67　(2)　149-149　2001/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  146. Analysis of the Factors inducing Necrotic Symptoms on Cucumber mosaic virus- infected Cowpea

    Takahashi C., Karasawa A., Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　67　(2)　149-149　2001/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  147. Identification of Tobacco Proteins that Bind to the Coat Protein of Cucumber mosaic virus Strain Y in the Yeast Two-Hybrid System

    Mitobe I., Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　67　(2)　149-150　2001/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  148. Mapping the Virus and Host Genes Involved in the Resistance Response in Cucumber Mosaic Virus-Infected Arabidopsis thaliana Peer-reviewed

    Hideki Takahashi, Masashi Suzuki, Keiko Natsuaki, Takuma Shigyo, Kumiko Hino, Tohru Teraoka, Daijiro Hosokawa, Yoshio Ehara

    Plant and Cell Physiology　42　(3)　340-347　2001/03/15

    DOI： 10.1093/pcp/pce039 　

    ISSN： 0032-0781

    eISSN： 1471-9053

  149. Mapping the virus and host genes involved in the resistance response in cucumber mosaic virus-infected Arabidopsis thaliana

    Hideki Takahashi, Masashi Suzuki, Keiko Natsuaki, Takuma Shigyo, Kumiko Hino, Tohru Teraoka, Daijiro Hosokawa, Yoshio Ehara

    Plant and Cell Physiology　42　(3)　340-347　2001

    Publisher: Japanese Society of Plant Physiologists

    DOI： 10.1093/pcp/pce039 　

    ISSN： 0032-0781

  150. Study on the Signal Transduction Pathway in the Resistance Response to Cucumber Mosaic Virus (II) : Resistance to CMV in nahG and npr1-5 Mutants(Abstracts Presented at the Meeting of the Tohoku Division)

    Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　66　(3)　265-265　2000/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  151. Time Course of Viral Replication in the Green Area of Cucumber mosaic virus-infected Tobacco Leaves

    Hase S., Karasawa A., Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　66　(2)　167-167　2000/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  152. Occurrence of Mutagenic Virus in Regenerated Plants from Tobacco Infected with the Yellow Strain of Cucumber Mosaic Virus

    Kurita S., Hase S., Karasawa A., Takahashi H., Ehara Y>

    Annals of the Phytopathological Society of Japan　66　(2)　166-167　2000/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  153. Study on the Signal Transduction Pathway in the Resistance Response to Cucumber mosaic virus (I) Arabidopsis thaliana ecotype

    Takahashi H., Hase S., Karasawa A., Ehara Y.

    Annals of the Phytopathological Society of Japan　66　(2)　150-150　2000/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  154. Ethylene Production in Tobacco Leaves Infected with Cucumber mosaic virus

    Kurosaka M, Hase S, Sato S, Takahashi H, Karasawa A, Ehara Y

    Annals of the Phytopathological Society of Japan　66　(2)　150-150　2000/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  155. キュウリモザイクウイルスに対する宿主応答とシグナル伝達

    高橋英樹, 江原淑夫

    平成12年度植物感染生理談話会論文集ー植物と微生物相互作用の夜明けー　36　103-120　2000/08

    Publisher: 日本植物病理学会

  156. Characterization of symptom determinants in two mutants of cucumber mosaic virus Y strain, causing distinct mild green mosaic symptoms in tobacco

    M. Sugiyama, H. Sato, A. Karasawa, S. Hase, H. Takahashi, Y. Ehara

    Physiological and Molecular Plant Pathology　56　(2)　85-90　2000

    Publisher: Academic Press

    DOI： 10.1006/pmpp.1999.0250 　

    ISSN： 0885-5765

  157. Novel mannose-binding rice lectin composed of some isolectins and its relation to a stress-inducible salT gene

    Ko Hirano, Tohru Teraoka, Homare Yamanaka, Akane Harashima, Akiko Kunisaki, Hideki Takahashi, Daijiro Hosokawa

    Plant and Cell Physiology　41　(3)　258-267　2000

    Publisher: Japanese Society of Plant Physiologists

    DOI： 10.1093/pcp/41.3.258 　

    ISSN： 0032-0781

  158. A novel satellite RNA of cucumber mosaic virus induces unique line-pattern mosaic symptoms in tobacco

    H. Sato, S. Hase, M. Sugiyama, A. Karasawa, T. Suzuki, H. Takahashi, Y. Ehara

    Journal of Phytopathology　148　(1)　47-51　2000

    DOI： 10.1046/j.1439-0434.2000.00469.x 　

    ISSN： 0931-1785

  159. Changes in Symptoms and Coat Protein (CP) Amino Acid Sequences by Sequential Passages of Cucumber Mosaic Virus CP Mutants on Cowpea Plants (Abstracts Presented at the Meeting of the Tohoku Division)

    Karasawa A., Sugiyama M., Satoh H., Ito K., Hase S., Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　65　(6)　665-665　1999/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  160. Analysis of a Cucumber Mosaic Virus Mutant Which Induces the Hypersensitive Response in Tobaccos (II) (Abstracts Presented at the Meeting of the Tohoku Division)

    Takahashi H., Sugiyama M., Suzuki T., Karasawa A., Hase S., Ehara Y.

    Annals of the Phytopathological Society of Japan　65　(6)　665-665　1999/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  161. Relationship between Etylene Production and Systemic Mosaic Symptoms in Tobacco Leaves Infected with Cucumber Mosaic Virus Strain Y (Abstracts Presented at the Meeting of the Tohoku Division)

    Hase S, Kurosaka M, Sato S, Takahashi H, Karasawa A, Ehara Y

    Annals of the Phytopathological Society of Japan　65　(6)　666-666　1999/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  162. ウイルス抵抗性発現におけるシグナル伝達−サリチル酸、ジャスモン酸、エチレンの関与

    高橋英樹

    日本植物病理学会東北部会35周年記念出版—東北地方における植物病理学のフロントライン　113-119　1999/10

    Publisher: 日本植物病理学会東北部会

  163. Development of the Green Area in Tobacco Leaves Infected with Cucumber Mosaic Virus Strain

    Hase S., Karasawa A., Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　65　(3)　384-384　1999/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  164. Correlation of Necrotic Symptoms with Viral Movement, Accumulation and Coat Protein Amino Acid in Cowpea Infected with Cucumber Mosaic Cucumovirus

    Karasawa A., Sasaki M., Hase S., Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　65　(3)　385-385　1999/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  165. Analysis of a Cucumber Mosaic Virus Mutant which Induces the Hypersensitive Resistance Response in Tobacco

    Takahashi H., Sugiyama M., Ito K., Karasawa A., Hase S., Ehara Y.

    Annals of the Phytopathological Society of Japan　65　(3)　384-385　1999/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  166. Localization of Cucumber Mosaic Virus RNA 3 and 4 in Infected Tabacco Plant Cells Revealed by Electron Microscopic in situ Hybridization

    Yokokawa T., Takahashi H., Ehara Y., Hosokawa D.

    Annals of the Phytopathological Society of Japan　65　(3)　332-332　1999/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  167. Identification of mRNA Species Showing Altered Expression Pattern in Cucumber Mosaic Virus-infected Cowpea

    Matsushita K., Karasawa A., Hase S., Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　65　(3)　385-385　1999/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  168. (8) Analysis of Satellite RNA in Regenerated Plants from Mosaic Leaves on Cucumber Mosaic Virus-infected Tobacco (Abstracts Presented at the Meeting of the Tohoku Division, 1998)

    Sato H., Sugiyama M., Suzuki T., Hase S., Karasawa A., Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　64　(6)　606-607　1998/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  169. (9) Occurrence of Mutagenic Virus in Regenerated Plants from Mosaic Leaves on Cucumber Mosaic Virus-infected Tobacco (Abstracts Presented at the Meeting of the Tohoku Division, 1998)

    Sugiyama M., Sato H., Hase S., Karasawa A., Takahashi H., Ehara Y.

    Annals of the Phytopathological Society of Japan　64　(6)　607-607　1998/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  170. (362) Amino Acid Sequence Associated with the Hyper-sensitive Response in Cucumber Mosaic Virus-infected Arabidopsis thaliana

    Takahashi H., Suzuki M., Teraoka T., Hosokawa D.

    Annals of the Phytopathological Society of Japan　64　(4)　417-417　1998/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  171. (361) Resistance and Susceptible Responses of 181 Arabidopsis thaliana Ecotypes to Cucumber Mosaic Virus Strain Y and Analysis of RCY Locus

    Yokokawa T., Goto N., Teraoka T., Hosokawa D., Takahashi H.

    Annals of the Phytopathological Society of Japan　64　(4)　417-417　1998/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  172. (102) Characterization and Cloning of Isolectins of the Mannose-binding Rice Lectin

    Hirano K., Teraoka T., Takahashi H., Hosokawa D.

    Annals of the Phytopathological Society of Japan　64　(4)　352-352　1998/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  173. (103) Interaction between the Mannose-binding Rice Lectin and the Concanavalin A-binding Glycoproteins Secreted from Germinating Conidia of Rice Blast Fungus

    Teraoka T., Nagaoka M., Hirano K, Takahashi H., Hosokawa D.

    Annals of the Phytopathological Society of Japan　64　(4)　352-353　1998/08/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  174. アラビドプシスにおけるCMV抵抗性

    高橋英樹

    植物ウイルス病研究会レポート　45　50-591　1998/04

    Publisher: 日本植物病理学会

  175. 植物の病害抵抗性反応におけるカタラーゼの役割

    高橋英樹

    化学と生物　36　212-213　1998/01

    Publisher: 学会出版センター

  176. ウイルスに対する宿主抵抗性

    高橋英樹

    植物細胞工学シリーズ8 分子レベルからみた植物の耐病性　182-188　1997/12

    Publisher: 秀潤社

  177. 植物の感染応答における情報伝達

    高橋英樹

    植物防疫　51　20-25　1997/12

    Publisher: 日本植物防疫協会

  178. (342) Molecular Mapping of Arabidopsis RCY1 Gene and Virus Avirulence Gene which Control the Resistance Response against Cucumber Mosaic Virus in Ecotype C24(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    SHIGYOU T., SUZUKI M., TERAOKA T., HOSOKAWA D., TAKAHASHI H.

    Annals of the Phytopathological Society of Japan　63　(3)　276-276　1997/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  179. (161) Possible Role of Proteoglycan(s) with Concanavalin A-binding Sugar Chain Secreted from Conidia at Germination of Rice Blast Fungus on Infection to Rice Plant(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    NAGAOKA M., TAMURA K., TERAOKA T., TAKAHASHI H., HOSOKAWA D.

    Annals of the Phytopathological Society of Japan　63　(3)　231-231　1997/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  180. Development of necrosis and activation of disease resistance in transgenic tobacco plants with severely reduced catalase levels

    H Takahashi, ZX Chen, H Du, YD Liu, DF Klessig

    PLANT JOURNAL　11　(5)　993-1005　1997/05

    DOI： 10.1046/j.1365-313X.1997.11050993.x 　

    ISSN： 0960-7412

  181. Infectivity of an Ordinary Strain of Potato Virus X (PVX-O) to Arabidopsis thaliana

    TAKAHASHI H., HINO K., XU L.X., TERAOKA T., HOSOKAWA D.

    Annals of the Phytopathological Society of Japan　62　(6)　615-615　1996/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  182. 病害抵抗性におけるサリチル酸を介した情報伝達機構

    高橋英樹, 寺岡 徹, 細川大二郎, Daniel F. Klessig

    平成8年度植物感染生理談話会論文集ー植物感染生理学研究の現状と将来展望ー　32　111-120　1996/07

    Publisher: 日本植物病理学会

  183. Protein Analysis of Isolectins of the Novel Mannose-Binding Rice Lectin

    HIRANO K., TERAOKA T., TAKAHASHI H., HOSOKAWA D.

    Annals of the Phytopathological Society of Japan　62　(3)　289-289　1996/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  184. Development of Necrosis and Activation of Disease Resistance in Transgenic Tobacco Plants with Severely Reduced Catalase Levels

    TAKAHASHI H., CHEN Z., LIU Y., KLESSIG D.F.

    Annals of the Phytopathological Society of Japan　62　(3)　344-344　1996/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  185. Isolation of T-DNA Tagged Genes Involving Symptom Expression on Virus-Infected Plants

    TAKAHASHI H., EHARA Y.

    Annals of the Phytopathological Society of Japan　60　(3)　387-388　1994/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  186. 植物ウイルスと病徴発現

    高橋英樹

    化学と生物　32　215-217　1994/05

    Publisher: 学会出版センター

  187. Phytogeography of vascular plants in Yakutia (Sakha)

    TAKAHASHI Hideki

    10　(1)　21-33　1994/03/20

    ISSN： 0911-6052

  188. Hypersensitive response in cucumber mosaic virus‐inoculated Arabidopsis thaliana

    Hideki Takahashi, Nobuharu Goto, Yoshio Ehara

    The Plant Journal　6　(3)　369-377　1994

    DOI： 10.1046/j.1365-313X.1994.06030369.x 　

    ISSN： 1365-313X 0960-7412

  189. The 35 kDa Protein Increased in Cowpea Leaves Infected with Cucumber Mosaic Virus

    SHIRASAWA N., TAKAHASHI H., EHARA Y.

    Annals of the Phytopathological Society of Japan　59　(6)　763-763　1993/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  190. Early Reactions of Hypersensitivity in Cucumber Mosaic Virus-Infected Cowpea Leaves : Correlation of Cell Membrane Damage with an O^-_2 Generating

    URIHARA I., KAWABE K., TAKAHASHI H., KARASAWA A., EHARA Y.

    Annals of the Phytopathological Society of Japan　59　(6)　763-763　1993/12/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  191. Analysis of Virus and Plant Genes Involving Local Necrotic Spot Symptom Formation on CMV-Inoculated Arabidopsis thaliana

    TAKAHASHI H., EHARA Y.

    Annals of the Phytopathological Society of Japan　59　(3)　340-340　1993/06/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  192. Response of Arabaidopsis thaliana 12 Ecotypes to Cucumber Mosaic Virus Infection

    TAKAHASHI H., EHARA Y., GOTO N.

    Annals of the Phytopathological Society of Japan　59　(1)　58-58　1993/02/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  193. Severe chlorotic spot symptoms in cucumber mosaic virus strain Y-infected tobaccos are induced by a combination of the virus coat protein gene and two host recessive genes.

    H. Takahashi, Y. Ehara

    Molecular plant-microbe interactions : MPMI　6　182-189　1993/01/01

    DOI： 10.1094/MPMI-6-182 　

    ISSN： 0894-0282

  194. Nucleotide Sequence of RNA 2 from Cucumber Mosaic Virus Strain 0

    HASE S., KARASAWA A., TAKAHASHI H., EHARA Y.

    Annals of the Phytopathological Society of Japan　58　(4)　632-633　1992/10/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  195. Preparation of Antibodies Reacting with the 2a Protein of Cucumber Mosaic Virus by Using E.coli-expressed Polypeptide

    OGAWARA T., TAKAHASHI H., KARASAWA A., EHARA Y.

    Annals of the Phytopathological Society of Japan　58　(4)　630-630　1992/10/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  196. Analysis of Virus and Host Genes Involving Symptom Expression on Cucumber Mosaic Virus Strain Y-Infected Tobacco Plants

    TAKAHASHI H., EHARA Y.

    Annals of the Phytopathological Society of Japan　58　(4)　628-628　1992/10/25

    Publisher: The Phytopathological Society of Japan (PSJ)

    ISSN： 0031-9473

  197. (13) CMV(Y)感染タバコ葉における光化学系 II・酸素発生系の活性低下 (東北部会)

    高橋 英樹, 江原 淑夫

    日本植物病理學會報　58　(1)　119-119　1992/01/25

    Publisher: 日本植物病理学会

    ISSN： 0031-9473

  198. Changes in the activity and the polypeptide composition of the oxygen-evolving complex in photosystem II of tobacco leaves infected with cucumber mosaic virus strain Y.

    H. Takahashi, Y. Ehara

    Molecular plant-microbe interactions : MPMI　5　269-272　1992/01/01

    DOI： 10.1094/MPMI-5-269 　

    ISSN： 0894-0282

  199. (262) CMV・Y 接種タバコ葉において黄色斑出現を支配するウイルス因子の解析 (日本植物病理学会大会)

    高橋 英樹, 江原 淑夫

    日本植物病理學會報　57　(3)　460-460　1991/07/25

    Publisher: 日本植物病理学会

    ISSN： 0031-9473

  200. A PROTEIN IN THE OXYGEN-EVOLVING COMPLEX IN THE CHLOROPLAST IS ASSOCIATED WITH SYMPTOM EXPRESSION ON TOBACCO-LEAVES INFECTED WITH CUCUMBER MOSAIC-VIRUS STRAIN-Y

    H TAKAHASHI, Y EHARA, H HIRANO

    PLANT MOLECULAR BIOLOGY　16　(4)　689-698　1991/04

    DOI： 10.1007/BF00023433 　

    ISSN： 0167-4412

  201. (211) CMV接種タバコ葉において病徴発現に伴い低下する2種の宿主タンパク質の性状解析 (平成2年度大会講演要旨)

    高橋 英樹, 平野 久, 江原 淑夫

    日本植物病理學會報　56　(3)　411-412　1990/07/25

    Publisher: 日本植物病理学会

    ISSN： 0031-9473

  202. (230) CMV接種タバコ葉タンパク質の2次元電気泳動による解析 (平成元年度日本植物病理学大会講演要旨)

    高橋 英樹, 江原 淑夫

    日本植物病理學會報　55　(4)　527-527　1989/10/25

    Publisher: 日本植物病理学会

    ISSN： 0031-9473

  203. DNA-SEQUENCE OF GENE-VI OF CAULIFLOWER MOSAIC-VIRUS JAPANESE STRAIN-S (CAMV S-JAPAN)

    H TAKAHASHI, K SHIMAMOTO, M SUZUKI, Y EHARA

    NUCLEIC ACIDS RESEARCH　17　(19)　7981-7981　1989/10

    DOI： 10.1093/nar/17.19.7981 　

    ISSN： 0305-1048

  204. CAULIFLOWER MOSAIC-VIRUS GENE-VI CAUSES GROWTH SUPPRESSION, DEVELOPMENT OF NECROTIC SPOTS AND EXPRESSION OF DEFENSE-RELATED GENES IN TRANSGENIC TOBACCO PLANTS

    H TAKAHASHI, K SHIMAMOTO, Y EHARA

    MOLECULAR & GENERAL GENETICS　216　(2-3)　188-194　1989/04

    DOI： 10.1007/BF00334355 　

    ISSN： 0026-8925

  205. (269) カリフラワーモザイクウイルス封入体タンパク質遺伝子のタバコ植物体への取込みと発現 (昭和63年度日本植物病理学会大会講演要旨)

    高橋 英樹, 島本 功, 鈴木 正彦, 江原 淑夫

    日本植物病理學會報　54　(3)　410-410　1988/07/25

    Publisher: 日本植物病理学会

    ISSN： 0031-9473

  206. ALTERED FRACTIONATION PROFILES OF HIGHER-PLANT POLY(A)-CONTAINING RNA BY SELECTIVE FORMATION OF A COMPLEX WITH A HYDROPHOBIC IMPURITY

    H TAKAHASHI, T NITTA

    PLANT SCIENCE　43　(1)　63-67　1986

    DOI： 10.1016/0168-9452(86)90109-3 　

    ISSN： 0168-9452

  207. (200) CMV感染タバコ葉における病微発現と宿主mRNAの量的質的変化 (昭和60年度 日本植物病理学会大会講演要旨)

    高橋 英樹, 江原 淑夫

    日本植物病理學會報　51　(3)　368-368　1985/07/25

    Publisher: 日本植物病理学会

    ISSN： 0031-9473

  208. (14) CMV 2系統に感染したタバコ葉における葉緑体のタンパクと構造の変化 (東北部会講演要旨)

    高橋 英樹, 江原 淑夫

    日本植物病理學會報　51　(1)　69-69　1985/01/25

    Publisher: 日本植物病理学会

    ISSN： 0031-9473

  209. (198) タバコ葉における CMV 2系統の増殖と宿主蛋白および核酸の変動 (昭和59年度 日本植物病理学会大会講演要旨)

    高橋 英樹, 江原 淑夫, 山中 達

    日本植物病理學會報　50　(3)　432-432　1984/07/25

    Publisher: 日本植物病理学会

    ISSN： 0031-9473

  210. SELECTIVE FORMATION OF A COMPLEX OF POLYADENYLATE WITH AN IMPURITY IN HIGHER-PLANT NUCLEIC-ACID PREPARATIONS

    T NITTA, H TAKAHASHI, K KOBAYASHI, K FUKUEI

    PLANT SCIENCE LETTERS　34　(1-2)　135-144　1984

    DOI： 10.1016/0304-4211(84)90136-6 　

    ISSN： 0304-4211

  211. 3Ea-12 高等植物におけるポリ(A)結合性植物の存在 : IV ウニ胚における類似物質との比較

    新田 毅, 高橋 英樹

    日本植物生理学会年会とシンポジウム講演要旨集　23　323-323　1983

    Publisher: 日本植物生理学会

  212. 植物の核酸研究を探る

    新田 毅, 高橋英樹

    遺伝　36　415-10　1982/04

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Books and Other Publications 11

  1. Antiviral Resistance in Plants: Methods and Protocols

    Yukiyo Sato, Hideki Takahashi

    Springer　2017

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    In Methods in Molecular Biology (Eds. Kobayashi, K. and Nishiguchi, M.)

  2. Plant Virology Protocols, Third Edition

    Takeaki Ishihara, Yukiyo Sato, Hideki Takahashi

    Springer　2014

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    2. Deep sequencing, meta-genome and microarray analysis for virus ecology, pathogenicity, and resistance (6) Microarray Analysis of R-gene-mediated Resistance to Viruses

  3. Genome-enabled integration of research in plant-pathogen systems

    Takahashi, H, Takenaka, S

    APS Press　2010/12

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    Eds. T. Walpert, T. Shiraishim, K. Akimitsu and J. Glazebrook

  4. 植物病理学

    眞山滋志, 難波成任

    文英堂　2010/04

  5. 微生物と植物の相互作用

    高橋英樹, 竹中重仁

    ソフトサイエンス社　2009/02/01

  6. 微生物の病原性と植物の防御応答

    高橋英樹, 石原岳明, 関根健太郎, 長谷 修

    北海道大学出版会　2007/02/28

  7. Genomics and Genetic Analysis of Plant Parasitism and Defense.

    Takahashi, H, Sekine, K. T, Ishihara, T, Hase, S, Ikegami, M, Shah, J

    APS Press　2004/12

  8. 作物保護の新展開−バイオサイエンスのかけはし−

    高橋英樹

    ソフトサイエンス社　2004/12

  9. 新版 分子レベルからみた植物の耐病性

    高橋英樹

    秀潤社　2004/07

  10. 応用生命科学のための生物学入門

    高橋英樹

    培風館　2003/03

  11. 新農学実験マニュアル

    高橋英樹

    ソフトサイエンス社　2002/03

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Presentations 192

  1. R遺伝子による認識を免れる変異型CMV CPのアラニンスキャニングによる探索

    平成30年度日本植物病理学会大会　2018/03/25

  2. R細胞間移行におけるCMVのMOIはR遺伝子による抵抗性誘導時に低下する

    平成30年度日本植物病理学会大会　2018/03/25

  3. Comparative analysis of two-type of cell death developed in cucumber mosaic virus-inoculated leaves of Arabidopsis thaliana

    平成30年度日本植物病理学会大会　2018/03/25

  4. シロイヌナズナへの無病徴感染に関わるキュウリモザイクウイルスのゲノムRNAと宿主因子の解析

    平成30年度日本植物病理学会大会　2018/03/25

  5. キュウリモザイクウイルスに無病徴感染したシロイヌナズナにおけるRNA-seq解析

    平成30年度日本植物病理学会大会　2018/03/25

  6. シロイヌナズナに無病徴感染するキュウリモザイクウイルスのゲノムRNAの機能解析

    平成29年度日本植物病理学会東北部会　2017/09/28

  7. シロイヌナズナcpl3変異体におけるNB-LRR型抵抗性タンパク質RCY1を介したキュウリモザイクウイルス抵抗性の亢進

    平成29年度日本植物病理学会東北部会　2017/09/28

  8. 植物に潜在感染しているウイルスの役割：ウイルスは植物の生存戦略に寄与できるか？

    環境微生物系学会合同大会2017　2017/08/29

  9. ハクサンハタザオから単離したキュウリモザイクウイルスの性状解析

    平成29年度日本植物病理学会大会　2017/04/26

  10. EMS変異誘発シロイヌナズナ系統におけるNB-LRRクラス抵抗性遺伝子RCY1プロモーター領域シトシンメチル化とCMV抵抗性レベル低下の相関

    平成29年度日本植物病理学会大会　2017/04/26

  11. ヒストン脱メチル化酵素変異体ldlにおけるキュウリモザイクウイルス抵抗性の解析

    平成29年度日本植物病理学会大会　2017/04/26

  12. RNAサイレンシング関連因子AGO2遺伝子のプライミングにおけるmiRNAの役割

    平成29年度日本植物病理学会大会　2017/04/26

  13. キュウリモザイクウイルスの欠失変異パターンの解析

    平成29年度日本植物病理学会大会　2017/04/26

  14. 植物ウイルスの多数決型意思決定システム

    平成29年度日本植物病理学会大会　2017/04/26

  15. ヒスチジンを処理した青枯病抵抗性台木品種・罹病性穂木品種の接ぎ木トマトにおける遺伝子発現変動の網羅的解析

    平成28年度日本植物病理学会東北部会　2016/09/29

  16. 抵抗性遺伝子RCY1過剰発現によるキュウリモザイクウイルス (CMV) 高度抵抗性が失われたシロイヌナズナ変異株の単離

    平成28年度日本植物病理学会東北部会　2016/09/29

  17. ハクサンハタザオに潜在感染しているCucumber mosaic virusの単離と全塩基配列の決定および病徴の解析

    平成28年度日本植物病理学会東北部会　2016/09/29

  18. CMV CPの多量体化・粒子形成に寄与するとされるαへリックスドメインはR遺伝子によるCPの認識に必要である

    平成28年度日本植物病理学会東北部会　2016/09/29

  19. CMV感染細胞内のMP機能欠損ゲノムの割合と隣接細胞への細胞間移行確率の関係

    平成28年度日本植物病理学会東北部会　2016/09/29

  20. 有機栽培育苗土から分離した糸状菌のイネ苗病害抑制効果の解析

    平成28年度日本植物病理学会東北部会　2016/09/29

  21. イネ有機栽培育苗土由来Pseudomonas属菌のイネもみ枯細菌病抑制効果におけるエチレンの関与

    平成28年度日本植物病理学会東北部会　2016/09/29

  22. イネ有機栽培育苗土由来Pseudomonas属菌の混合施用による病害抑制機構の解析

    平成28年度日本植物病理学会東北部会　2016/09/29

  23. イネ有機栽培育苗土由来の培養可能細菌を混合施用した際の病害抑制効果と微生物相変動の関係

    平成28年度日本植物病理学会東北部会　2016/09/29

  24. プライミング時におけるRNAサイレンシング関連因子AGO2のプロモーター領域のクロマチン構造変化とRNA Polymerase II結合性

    安藤杉尋, Jaskiewicz Michal・Conrath Uwe, 宮下脩平, 高橋英樹

    平成28年度日本植物病理学会大会　2016/03/21

  25. miRNAによるRNAサイレンシング関連因子AGO2及びAGO3のプライミングの制御機構の解析

    大谷峻, 定池歩美, 宮下脩平, 高橋英樹, 安藤杉尋

    平成28年度日本植物病理学会大会　2016/03/21

  26. キュウリモザイクウイルスの細胞間移行におけるRNA3のMOI(multiplicity of infection)の推定

    鈴木万智, 安藤杉尋, 高橋英樹, 宮下脩平

    平成28年度日本植物病理学会大会　2016/03/21

  27. トマトモザイクウイルスのゲノム／サブゲノムRNA合成確率の比を制御するシスエレメント

    宮下脩平, 安藤杉尋, 高橋英樹, 石川雅之

    平成28年度日本植物病理学会大会　2016/03/21

  28. R遺伝子による細胞死誘導の回避に寄与するキュウリモザイクウイルス外被タンパク質の1アミノ酸変異はウイルスの全身移行能を喪失させる

    安藤由起, 宮下脩平, 安藤杉尋, 高橋英樹

    平成28年度日本植物病理学会大会　2016/03/21

  29. アミノ酸類処理した青枯病菌抵抗性トマト台木品種と罹病性穂木品種における遺伝子発現変動の網羅的比較解析

    高橋英樹, 大川佳織, 中保一浩, 安藤杉尋, 宮下脩平, 瀬尾茂美

    平成28年度日本植物病理学会大会　2016/03/21

  30. イネ種子由来細菌のイネもみ枯細菌病菌およびイネ苗立枯細菌病菌に対する抗菌活性の解析

    石宮純, 吉田重信, 對馬誠也, 宮下脩平, 高橋英樹, 安藤杉尋

    平成27年度日本植物病理学会東北部会　2015/09/28

  31. イネ有機栽培育苗土から分離したPseudomonas sp.施用時のイネ幼苗における防御関連遺伝子の発現解析

    鎗山純, 吉田重信, 對馬誠也, 宮下脩平, 高橋英樹, 安藤杉尋

    平成27年度日本植物病理学会東北部会　2015/09/28

  32. イネ有機栽培育苗土の苗いもち抑制効果の検討

    笠原雅美, 宮下脩平, 高橋英樹, 安藤杉尋

    平成27年度日本植物病理学会東北部会　2015/09/28

  33. 異なる有機栽培育苗土の微生物コミュニティーによるイネもみ枯細菌病抑制効果の比較

    安藤杉尋, 鎗山純, 小林隆, 吉田重信, 對馬誠也, 伊藤豊彰, 宮下脩平, 高橋英樹

    平成27年度日本植物病理学会東北部会　2015/09/28

  34. 次世代シークエンサーを用いたイネ有機栽培育苗培土の微生物多様性解析

    高橋英樹, 松下裕子, 伊藤豊彰, 小林 隆, 橋本知義, 宮下脩平, 守川俊幸, 吉田重信, 對馬誠也, 安藤杉尋

    平成27年度日本植物病理学会東北部会　2015/09/28

  35. トマトモザイクウイルスのゲノム／サブゲノム合成量比を制御するシスエレメント

    宮下脩平, 安藤杉尋, 高橋英樹, 石川雅之

    平成27年度日本植物病理学会東北部会　2015/09/28

  36. Intron-mediated enhancement of disease resistance gene expression in Arabidopsis thaliana International-presentation

    CFAI opening ceremony, special lectures, and orientation　2015/05/12

  37. RCY1/HRT/RPP8複合抵抗性遺伝子座を利用した組換えウイルス抵抗性遺伝子とランダム変異導入外被タンパク質遺伝子を用いたCMV抵抗性の分子基盤解析

    安藤由起, 安藤杉尋, 高橋英樹

    平成27年度日本植物病理学会大会　2015/03/29

  38. 青枯病菌抵抗性トマト台木品種LS-89における過敏感反応抵抗性の関与

    高橋英樹, 中保一浩, 瀬尾茂美, 安藤杉尋

    平成27年度日本植物病理学会大会　2015/03/29

  39. トマト青枯病抑制効果をもつ3種の昆虫病原微生物資材を処理したトマト主根における網羅的遺伝子発現解析

    大川佳織, 安藤杉尋, 清水将文, 百町満朗, 岩本豊, 相野公孝, 松浦克成, 後藤新一, 對馬誠也, 吉田重信, 高橋英樹

    平成27年度日本植物病理学会大会　2015/03/29

  40. キュウリモザイクウイルス抵抗性誘導時のRNAサイレンシング関連因子AGO2のプライミングに対するヒストン修飾機構の関与

    安藤杉尋, Michal Jaskiewicz, 高橋英樹, Uwe Conrath

    平成27年度日本植物病理学会大会　2015/03/29

  41. Intron-mediated enhancement of disease resistance gene expression in Arabidopsis thaliana International-presentation

    Seminar in Wageningen University　2015/03/10

  42. Intron-mediated enhancement of disease resistance gene expression in Arabidopsis thaliana International-presentation

    Seminar in Utrecht University　2015/03/09

  43. Seed-disinfection using plasma irradiation for suppression of rice diseases. International-presentation

    Ando, S, Ochi, A, Tsushima, S, Konishi, H, Kaneko, T, Takahashi, H

    1st E-MRS/MRS-J Bilateral Sumposia　2014/12/10

  44. Molecular basis of RCY1/RPP8 locus-conferred dual resistance to Cucumber mosaic virus and Hyaloperonospora arabidopsidis International-presentation

    高橋英樹, 鈴木 匡, 竹下 稔, 増田 税, 安藤杉尋

    3rd Japan-Korea Joint Symposium　2014/10/23

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    本人の講演

  45. 微生物防除資材を用いたトマト青枯病抑制の分子基盤

    高橋英樹, 安藤杉尋, 清水将文, 百町満朗, 岩本 豊, 相野公孝, 松浦克成, 浅野峻介, 平山喜彦, 對馬誠也, 吉田重信

    第２６回植物細菌病談話会　2014/10/09

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    本人の講演

  46. CC-NB-LRR型キュウリモザイクウイルス抵抗性遺伝子RCY1における選択的スプライシング転写産物の検出

    佐藤有希代, 安藤杉尋, 高橋英樹

    平成26年度日本植物病理学会東北部会　2014/09/24

  47. BTH処理によるキュウリモザイクウイルス抵抗性誘導時のWRKY33, WRKY46, WRKY70のプライミングの解析

    望月星, 大日方敦哉, Michal Jaskiewicz, 高橋英樹, Uwe Conrath, 安藤杉尋

    平成26年度日本植物病理学会東北部会　2014/09/24

  48. プラズマ照射による細菌性イネ苗病害の抑制技術の検

    安藤杉尋, 菅野剛, 小西秀明, 對馬誠也, 吉田重信, 守川俊幸, 金子俊郎, 高橋英樹

    平成26年度日本植物病理学会東北部会　2014/09/24

  49. イネ種子由来細菌によるイネもみ枯細菌抑制効果の解析

    石宮純, 對馬誠也, 吉田重信, 高橋英樹, 安藤杉尋

    平成26年度日本植物病理学会東北部会　2014/09/24

  50. 有機栽培育苗土由来土壌細菌の混合施用によるイネもみ枯細菌病抑制効果の検討

    鎗山純, 對馬誠也, 吉田重信, 伊藤豊彰, 高橋英樹, 安藤杉尋

    平成26年度日本植物病理学会東北部会　2014/09/24

  51. プラズマ照射によるイネばか苗病の防除効果

    越智昭彦, 小西秀明, 安藤杉尋, 佐藤健二, 横山克至, 金子俊郎, 高橋英樹

    平成26年度日本植物病理学会東北部会　2014/09/24

  52. イネ有機栽培育苗培土におけるもみ枯細菌病抑制と土壌理化学性および微生物多様性の関係

    高橋英樹, 小林 隆, 伊藤豊彰, 松下裕子, 對馬誠也, 橋本知義, 本多健一郎, 安藤杉尋

    平成26年度日本植物病理学会東北部会　2014/09/24

  53. Molecular basis for bioinsecticide-activated plant defense system suppressing bacterial wilt diseases caused by Ralstonia solanacearum International-presentation

    Hideki Takahashi, Mitsuro Hyakumachi, Masafumi Shimizu, Yutaka Iwamoto, Masataka Aino, Katsuhiko Matsuura, Shinichi Goto, Kazuhiro Nakano, Shugihiro Ando, Tsutomu Arie, Seya Tsushima, Shigenobu Yoshida

    13th IUPAC International Congress of Pesticide Chemistry　2014/08/10

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    本人の講演

  54. NB-LRR型キュウリモザイクウイルス抵抗性タンパク質RCY1の蓄積を増強するイントロンの役割

    佐藤有希代, 安藤杉尋, 高橋英樹

    日本植物病理学会第49回植物感染生理談話会　2014/08/06

  55. キュウリモザイクウイルス抵抗性誘導時に発現上昇するWRKY遺伝子のプライミングの解析

    日本植物病理学会第49回植物感染生理談話会　2014/08/06

  56. キュウリモザイクウイルス抵抗性遺伝子RCY1に対するウイルス非病原性因子の機能ドメイン解析

    安藤由起, 安藤杉尋, 高橋英樹

    日本植物病理学会第49回植物感染生理談話会　2014/08/06

  57. 植物免疫におけるプライミングとクロマチン修飾機構の解析

    安藤杉尋, Michal Jaskiewicz, 高橋英樹, Uwe Conrath

    日本植物病理学会第49回植物感染生理談話会　2014/08/06

  58. ANALYSIS OF PATHOGEN-RECOGNITION DOMAIN IN NB-LRR CLASS RCY1/RPP8 LOCUS CONFERRING DUAL RESISTANCE TO CUCUMBER MOSAIC VIRUS AND HYALOPERONOSPORA ARABIDOPSIDIS IN ARABIDOPSIS THALIANA International-presentation

    Hideki Takahashi, Yuki Ando, Sugihiro Ando

    XVI International Congress on Molecular Plant-Microbe Interactions　2014/07/06

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    本人の発表

  59. Role of Intron-Mediated Enhancement on Accumulation of an Arabidopsis NB-LRR Class R-protein That Confers Resistance to Cucumber mosaic virus International-presentation

    Yukiyo Sato, Sugihiro Ando, Hideki Takahashi

    XVI International Congress on Molecular Plant-Microbe Interactions　2014/07/06

  60. Study on priming and chromatin modification in plant immune systems International-presentation

    Ando, S, Jaskiewicz, M, Takahashi, H, Conrath, U

    XVI International Congress on Molecular Plant-Microbe Interactions　2014/07/06

  61. キュウリモザイクウイルス・アブラナ科べと病菌複合抵抗性を支配するRCY1/RPP8遺伝子座の組換えと病原体応答

    高橋英樹, 安藤杉尋

    平成26年度日本植物病理学会大会　2014/06/02

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    本人の発表

  62. BTH処理によるキュウリモザイクウイルス抵抗性とRNAサイレンシング関連因子のプライミングの解析

    安藤杉尋, Jaskiewicz Michal, 高橋英樹, Conrath Uwe

    平成26年度日本植物病理学会大会　2014/06/02

  63. キュウリモザイクウイルス抵抗性遺伝子RCY1に対するウイルス非病原性因子の機能ドメイン解析

    安藤由起, 安藤杉尋, 高橋英樹

    平成26年度日本植物病理学会大会　2014/06/02

  64. NB -LRR型キュウリモザイクルス抵抗性タンパ質の蓄積を制御するイントロン配列の役割

    佐藤有希代, 安藤杉尋, 高橋英樹

    平成26年度日本植物病理学会大会　2014/06/02

  65. 有機栽培イネと育苗培土に由来する微生物の解析と病害防除

    高橋英樹, 安藤杉尋, 伊藤豊彰, 松下裕子, 對馬誠也, 本多健一郎

    第14 回日本有機農業学会(仙台)大会　2013/12/07

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    本人の講演

  66. 昆虫病原性糸状菌Paecilomyces tenuipesおよびBeauveria bassianaの培養濾液を施用したトマトにおける青枯病抑制効果と防御応答遺伝子発現の解析

    高橋英樹, 安藤杉尋, 清水将文, 百町満朗, 岩本 豊, 相野公孝, 松浦克成, 浅野峻介, 平山喜彦, 對馬誠也, 吉田重信

    平成25年度日本植物病理学会東北部会　2013/09/24

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  67. キュウリモザイクウイルス抵抗性遺伝子RCY1の発現調節機構の解析

    佐藤有希代, 安藤杉尋, 高橋英樹

    平成25年度日本植物病理学会東北部会　2013/09/24

  68. イネ有機育苗培土およびコンポスト施用による苗病害抑制の解析

    菅野 剛, 安藤杉尋, 伊藤豊彰, 對馬誠也, 守川俊幸, 本多健一郎, 中井 裕, 高橋英樹

    平成25年度日本植物病理学会東北部会　2013/09/24

  69. Bacillus thuringiensis を施用したトマト主根と側根組織における青枯病細菌の分布と網羅的遺伝子発現解析

    和田拓己, 安藤杉尋, 吉田重信, 對馬誠也, 百町満郎, 高橋英樹

    平成25年度日本植物病理学会東北部会　2013/09/24

  70. キュウリモザイクウイルス抵抗性遺伝子RCY1に対応するウイルス非病原性因子の解析

    高橋英樹, 鈴木 匡, 竹下 稔, 増田 税, 安藤杉尋

    平成25年度日本植物病理学会大会　2013/03/28

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  71. キュウリモザイクウイルス抵抗性遺伝子RCY1の発現調節機構の解析

    佐藤有希代, 安藤杉尋, 高橋英樹

    平成25年度日本植物病理学会大会　2013/03/28

  72. キュウリモザイクウイルス抵抗性遺伝子RCY1の抵抗性機構におけるWRKY70遺伝子の機能解析

    安藤杉尋, 大日方敦哉, 高橋英樹

    平成25年度日本植物病理学会大会　2013/03/28

  73. 有機栽培イネ細胞間隙に由来する微生物の解析と病害防除

    有機農業研究者会議2012　2012/10/24

  74. Bacillus thuringiensis subsp. sotto を施用したトマトの各組織におけるPR-1 遺伝子発現の比較解析

    平成24年度日本植物病理学会東北部会　2012/09/13

  75. ハクサイ根こぶ形成時におけるBrNIT2 遺伝子の選択的転写開始を制御するプロモーターの活性領域の解析

    平成24年度日本植物病理学会東北部会　2012/09/13

  76. イネ有機栽培育苗土の病害抑制効果の解析

    平成24年度日本植物病理学会東北部会　2012/09/13

  77. キュウリモザイクウイルス防御応答におけるNB-LRRタンパク質の機能解析

    日本植物病理学会植物感染生理談話会　2012/08/30

  78. RCY1-mediated Resistance to Cucumber Mosaic Virus Is Regulated by LRR Domain-mediated Interaction with CMV(Y) Following Degradation of RCY1 International-presentation

    Hideki Takahashi, Hiromi Shoji, Sugihiro Ando, Minoru Takeshita, Masashi Suzuki, Chikara Masuta

    XV International Congress of Molecular Plant-Microbe Interactions　2012/07/29

  79. キュウリモザイクウイルス感染に対するLRRドメインを介した抵抗性タンパク質RCY1の自己分解と防御応答システムの活性化

    高橋英樹, 庄子紘美, 竹下 稔, 鈴木 匡, 増田 税, 安藤杉尋

    平成24年度日本植物病理学会大会　2012/03/28

  80. Auto-activation of defense reaction in Nicotiana benthamiana by over-expression of RCY1 encoding CC-NB-LRR class R protein for the resistance to Cucumber mosaic virus International-presentation

    Hiromi Shoji, Sugihiro Ando, Hideki Takahashi

    The 2nd Japan-Korea Joint Symposium　2012/03/27

  81. Bacillus thuringiensis subsp. sotto を施用したトマトにおける防御関連遺伝子の発現解析

    高橋英樹, 荒川竜行, 西村光由, 安藤杉尋, 吉田重信, 對馬誠也, 百町満朗

    平成23年度日本植物病理学会東北部会　2011/10/30

  82. 有機栽培育苗土によるイネもみ枯細菌病抑制効果の解析

    安藤杉尋, 對馬誠也, 吉田重信, 長谷川浩, 小林隆, 伊藤豊彰, 高橋英樹

    平成23年度日本植物病理学会東北部会　2011/10/30

  83. キュウリモザイクウィルス抵抗性遺伝子RCY1 形質転換Nicotiana benthamiana のウィルス感染に対する応答

    庄子紘美, 安藤杉尋, 高橋英樹

    平成23年度日本植物病理学会東北部会　2011/10/30

  84. 生物防除微生物Pythium oligandrum菌体細胞壁エリシターによるイネの防御応答

    長谷 修, 生井恒雄, 竹中重仁, 高橋英樹

    平成23年度日本植物病理学会東北部会　2011/10/30

  85. Cyclic nucleotide-gated ion channel-mediated cell death may not play a critical for R geneconferred resistance to Cucumber mosaic virus in Arabidopsis thaliana International-presentation

    Hideki Takahashi, Sugihiro Ando, Yoshinori Kanayama

    International Congress of Virology 2011　2011/09/12

  86. トウモロコシおよびシロイヌナズナにおけるキュウリモザイクウイルス抵抗性応答と遺伝子発現プロファイルの比較解析

    高橋英樹, 安藤杉尋, 間野吉郎

    平成23年度日本植物病理学会大会　2011/03/27

  87. キュウリモザイクウイルス抵抗性遺伝子RCY1のタンパク質相互作用の解析

    高橋英樹, 千葉ゆか, 安藤杉尋

    平成23年度日本植物病理学会大会　2011/03/27

  88. 有機栽培イネからの内生細菌の分離と16SrDNA断片塩基配列による解析

    高橋英樹, 安藤杉尋, 伊藤豊彰, 長谷川 浩, 竹中重仁

    平成22年度日本植物病理学会東北部会　2010/10/04

  89. 有機栽培イネ地上部より単離された内生細菌のイネいもち病抵抗性への影響

    安藤杉尋, 伊藤豊彰, 長谷川浩, 竹中重仁, 長谷修, 生井恒雄, 高橋英樹

    平成22年度日本植物病理学会東北部会　2010/10/04

  90. キュウリモザイクウイルス抵抗性遺伝子RCY1を導入したNicotiana benthamianaの作出とウイルス感染に対する応答

    平成22年度日本植物病理学会大会　2010/04

  91. 外被タンパク質にアミノ酸変異を導入したキュウリモザイクウイルス(CMV)感染トマトにおける病徴発現の解析

    平成22年度日本植物病理学会大会　2010/04

  92. キュウリモザイクウイルス抵抗性遺伝子RCY1導入Nicotiana benthamianaとCMVベクターによるVIGSシステムの構築とRCY1-CMV抵抗性シグナル伝達系解析への利用

    平成22年度日本植物病理学会大会　2010/04

  93. Defense System Induced by Elicitin-like Proteins of the Biocontrol Agent Pythium oligandrum International-presentation

    10th US-Japan Science Seminar, “Genome-enaabled Integration of Research in Plant-Pathogen Systems　2010/01/24

  94. Metagenomic analysis of microbial communities in intercellular fluid of rice International-presentation

    Hideki Takahashi, Hiroyuki Sekiguchi, Toyoaki Ito, Tsuyoshi Sasahara, Noriko Hatanaka, Atsushi Ooba, Shu Hase, Yoshinori Yamauchi, Sugihiro Ando, Hiroshi Hasegawa, Shigehito Takenaka

    The 1st Japan-Korea Joint Symposium　2009/10/28

  95. 品種, 地域, 生育時期, 栽培条件が異なるイネ地上部の内生菌集団から増幅された16S rDNA-ITS断片のPCR-DGGE法による比較解析

    平成21年度日本植物病理学会東北部会　2009/09/29

  96. ハクサイ根こぶ形成時におけるBrNIT2タンパク質の蓄積レベルの解析

    平成21年度日本植物病理学会東北部会　2009/09/29

  97. Characterization of NON-RCY1-RESISTANCE (NRR) locus required for RCY1-conferred resistance to Cucumber mosaic virus in Arabidopsis International-presentation

    XIV. International Congress of Molecular Plant-Microbe Interaction (MPMI 2009)　2009/07/19

  98. キュウリモザイクウイルス抵抗性遺伝子RCY1を導入したシロイヌナズナエコタイプDi-17における乾燥ストレス耐性と矮化形態を制御する遺伝子のマッピング

    平成21年度日本植物病理学会大会　2009/03/26

  99. キュウリモザイクウイルス抵抗性遺伝子RCY1を過剰発現するシロイヌナズナエコタイプDi-17における非生物ストレス耐性の付与

    平成20年度日本植物病理学会東北部会　2008/09/25

  100. 高速シークエンスを用いたイネ細胞間隙に由来する微生物群の16SrDNA・18SrDNA断片の網羅的解析

    平成20年度日本植物病理学会東北部会　2008/09/25

  101. GFPレポーターを用いたキュウリモザイクウイルス抵抗性におけるサリチル酸シグナル伝達系活性化の解析

    甲斐朝湖, 関根健太郎, 河村陽子, 長谷修, 山下真生, 富田啓文, 高橋英樹

    平成20年度日本植物病理学会大会　2008/04/26

  102. キュウリモザイクウイルス抵抗性の強度を決定する抵抗性遺伝子産物RCY1の蓄積量

    関根健太郎, 川上さやか, 長谷修, 高橋英樹

    平成20年度日本植物病理学会大会　2008/04/26

  103. 生物防除微生物pythium oligandrumによる誘導抵抗性におけるシアノアラニンの役割

    高橋翔, 長谷修, 河村陽子, 竹中重仁, 高橋英樹

    平成20年度日本植物病理学会大会　2008/04/26

  104. イネ細胞間隙に由来する微生物群からのDNA単離と18rDNAの解析

    山内芳紀, 川上さやか, 関根健太郎, 長谷修, 伊藤豊彰, 畑中教子, 笹原剛志, 関口博之, 竹中重仁, 高橋英樹

    平成20年度日本植物病理学会大会　2008/04/26

  105. 生物防除微生物Pythium oligandrumによる誘導抵抗性の分子機構解明：シアノアラニン処理におけるジャスモン酸シグナル伝達経路の活性化

    高橋翔, 長谷修, 竹中重仁, 高橋英樹

    平成19年度日本植物病理学会東北部会　2007/09/20

  106. プラントアクティベーター及びエチレン誘導性のシアノアラニン合成酵素遺伝子プロモーターとGUSコード領域を導入した形質転換シロイヌナズナの作出と評価

    関根健太郎, 高橋翔, 甲斐朝湖, 長谷修, 山下真生, 富田啓文, 高橋英樹

    平成19年度日本植物病理学会東北部会　2007/09/20

  107. INF1処理と的における遺伝子発現の網羅的解析とエチレンシグナル伝達系の活性化

    河村陽子, 長谷修, 吉岡博文, 竹中重仁, 高橋英樹

    平成19年度日本植物病理学会東北部会　2007/09/20

  108. タバコN発現遺伝子による過敏感反応に伴う発熱反応

    高橋翼, 磯貝雅道, 吉川信幸

    平成19年度日本植物病理学会東北部会　2007/09/20

  109. イネ細胞間隙に由来する微生物群からのDNA単離と16SrDNAの解析

    高橋英樹, 関口博之, 山内芳紀, 川上さやか, 関根健太郎, 長谷修, 竹中重仁

    平成19年度日本植物病理学会東北部会　2007/09/20

  110. INF1 treatment activates the jasmonic acid-mediated signaling pathway and induces resistance to Ralstonia solanacearum in tomato International-presentation

    Yoko KAWAMURA, Shu HASE, Hirofumi YOSHIOKA, Shigehito TAKENAKA, Hideki TAKAHASHI

    XIII. International Congress of Molecular Plant-Microbe Interaction (MPMI 2007)　2007/07/21

  111. Functional analysis of LeATL6 encoding fungal glycoprotein elicitor-responsive ubiquitin ligase in tomato International-presentation

    Hideki TAKAHASHI, Daisuke HONDO, Shu HASE, Shigehito TAKENAKA

    XIII. International Congress of Molecular Plant-Microbe Interaction (MPMI 2007)　2007/07/21

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  112. High-level expression of a resistance gene, RCY1 confers extreme resistance to Cucumber mosaic virus International-presentation

    Ken-Taro SEKINE, Sayaka KAWAKAMI, Shu, HASE, Jyoti, SHAH, Hideki TAKAHASHI

    XIII. International Congress of Molecular Plant-Microbe Interaction (MPMI 2007)　2007/07/21

  113. WRKY33 negatively regulates the resistance to RCY1-mediated resistance to Cucumber mosaic virus in Arabidopsis thaliana International-presentation

    Tomoe OSANAI, Ken-Taro SEKINE, Takeaki ISHIHARA, Sayaka KAWAKAMI, Shu HASE, Hideki TAKAHASHI

    XIII. International Congress of Molecular Plant-Microbe Interaction (MPMI 2007)　2007/07/21

  114. Molecular mechanism for RCY1 conferred resistance to Cucumber mosaic virus in Arabidopsis thaliana International-presentation

    Takahashi, H, Sekine, K.-T, Kawakami, S, Osanai, T, Hase, S, Shah, J

    KCIST 2007 Symposium on Molecular Host-Parasite Interactions　2007/07/11

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  115. 生物防除微生物Pythium oligandrum による誘導抵抗性発現トマトの青枯病菌接種後における網羅的遺伝子発現解析

    長谷 修, 石原岳明, 中保一浩, 津金胤昭, 柴田大輔, 竹中重仁, 高橋英樹

    平成19年度日本植物病理学会大会　2007/03/28

  116. 糸状菌糖タンパク質エリシターにより発現誘導されるユビキチンリガーゼLeATL6と相互作用するタンパク質の解析

    高橋英樹, 本堂大祐, 長谷 修, 竹中重仁

    平成19年度日本植物病理学会大会　2007/03/28

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  117. キュウリモザイクウイルス高度抵抗性を誘導する抵抗性遺伝子RCY1の高レベル発現

    関根健太郎, 石原岳明, 川上さやか, 長谷 修, 高橋英樹

    平成19年度日本植物病理学会大会　2007/03/28

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  118. キュウリモザイクウイルスサイレンシングベクターを用いたトマトACD2の解析

    石原岳明, 高橋英樹, 増田 税

    平成19年度日本植物病理学会大会　2007/03/28

  119. WRKY33過剰発現シロイヌナズナのキュウリモザイクウイルスに対する応答

    小山内朋絵, 関根健太郎, 石原岳明, 川上さやか, 長谷 修, 高橋英樹

    平成19年度日本植物病理学会大会　2007/03/28

  120. INF1処理トマトにおける防御関連遺伝子の発現解析

    河村陽子, 長谷 修, 吉岡博文, 竹中重仁, 高橋英樹

    平成19年度日本植物病理学会大会　2007/03/23

  121. Analysis of transcriptome for PAMPs-induced disease resistance in tomato International-presentation

    Hideki TAKAHASHI, Daisuke HONDO, Shu HASE, Shigehito TAKENAKA

    The 3rd Kazusa Tomato Workshop　2007/03/20

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  122. 生物防除微生物Pythium oligandrumによって誘導されるLeATL6が活性化するジャスモン酸シグナル系の解析

    本堂大祐, 長谷 修, 竹中重仁, 高橋英樹

    平成18年度日本植物病理学会東北部会　2006/09/28

  123. EDS5過剰発現シロイヌナズナにおけるサリチル酸依存および非依存シグナル伝達を介したウイルスに対する基礎的抵抗性の誘導

    石原岳明, 関根健太郎, 長谷 修, Jyoti Shah, 高橋英樹

    平成18年度日本植物病理学会東北部会　2006/09/28

  124. シロイヌナズナエコタイプColumbiaにRCY1遺伝子を導入した形質転換植物におけるキュウリモザイクウイルス抵抗性

    関根健太郎, 石原岳明, 川上さやか, 長谷修, 高橋英樹

    平成18年度日本植物病理学会東北部会　2006/09/28

  125. シロイヌナズナエコタイプEr-0, Sf-2, Var2-1のRPP8/HRT/RCY1遺伝子座における構造解析

    川上さやか, 関根健太郎, 石原岳明, 長谷修, 高橋英樹

    平成18年度日本植物病理学会東北部会　2006/09/28

  126. ダイコンモザイクウイルスのゲノムRNA構造解析と東北地方における発生分布

    星 朱香, 関根健太郎, 佐藤加奈子, 本堂大祐, 長谷 修, 佐野輝男, 高橋英樹

    平成18年度日本植物病理学会東北部会　2006/09/27

  127. EDS5過剰発現シロイヌナズナの作出とキュウリモザイクウイルスに対する応答

    石原岳明, 関根健太郎, 長谷 修, 高橋英樹

    平成18年度日本植物病理学会東北部会　2006/09/27

  128. The role of ethylene and jasmonate signaling in bacterial wilt disease resistance of tomato induced by Pythium oligandrum International-presentation

    Shu Hase, Shigehito Takenaka, Kazuhiro Nakaho, Tsutomu Arie, Hideki Takahashi

    8th International Congress of Plant Molecular Biology (ISPMB 2006)　2006/08/20

  129. Beta-cyanoalanine synthase as a molecular marker for induced resistance by fungal glycoprotein elicitor and commercial plant activators International-presentation

    Hideki Takahashi, Takeaki Ishihara, Shu Hase, Ayaka Chiba, Kazuhiro Nakaho, Tsutomu Arie, Tohru Teraoka, Michiaki Iwata, Taneaki Tugane, Daisuke Shibata, Shigehito Takenaka

    8th International Congress of Plant Molecular Biology (ISPMB 2006)　2006/08/20

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  130. Diversity at RPP8/HRT/RCY1 locus containing CC-NB-LRR class R gene in Arabidopsis thaliana International-presentation

    Sayaka Kawakami, Ken-Taro Sekine, Takeaki Ishihara, Shu Hase, Jyoti Shah, Hideki Takahashi

    8th International Congress of Plant Molecular Biology (ISPMB 2006)　2006/08/20

  131. Single amino acid substitutions on Cucumber mosaic virus resistance gene RCY1 compromise resistance to virus but differentially suppress hypersensitive cell death in Arabidopsis thaliana International-presentation

    Ken-Taro Sekine, Takeaki Ishihara, Shu Hase, Tomonobu Kusano, Jyoti Shah, Hideki Takahashi

    8th International Congress of Plant Molecular Biology (ISPMB 2006)　2006/08/20

  132. Pythium oligandrumが発現誘導するRING-H2 zinc-finger proteinの機能解析

    本堂大祐, 長谷 修, 金山善則, 吉川信幸, 竹中 重仁, 高橋英樹

    日本植物病理学会 平成18年度植物感染生理談話会　2006/08/17

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  133. 生物防除微生物Pythium oligandrum によって誘導されるトマト青枯病抵抗性発現におけるジャスモン酸シグナル伝達系の関与

    長谷 修, 中保一浩, 有江 力, 瀬尾 茂美, 大橋祐子, 竹中 重仁, 高橋英樹

    平成18年度日本植物病理学会大会　2006/06/03

  134. Pythium oligandrumが発現誘導するRING-H2 zinc-finger proteinの機能解析

    本堂大祐, 長谷 修, 竹中 重仁, 高橋英樹

    平成18年度日本植物病理学会大会　2006/06/03

  135. 生物防除微生物Pythium oligandrumの卵胞子懸濁液および細胞壁タンパクエリシターを処理したトマト・イネ・ジャガイモにおける遺伝子発現変動の解析

    清水綾乃, 長谷 修, 石原岳明, 柴田大輔, 竹中重仁, 高橋英樹

    平成18年度日本植物病理学会大会　2006/06/03

  136. 生物防除微生物Pythium oligandrum およびプラントアクティベーター処理トマトにおけるBeta-cyanoalanine synthase 遺伝子の発現と誘導抵抗性マーカーとしての利用

    高橋英樹, 石原岳明, 長谷 修, 中保一浩, 有江 力, 寺岡 徹, 岩田道顕, 津金胤昭, 柴田大輔, 竹中重仁

    平成18年度日本植物病理学会大会　2006/06/03

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  137. CC-NBS-LRR型抵抗性遺伝子をコードするRPP8/HRT/RCY1 遺伝子座のシロイヌナズナ72 エコタイプにおける比較解析

    川上さやか, 関根健太郎, 石原岳明, 長谷修, 高橋英樹

    平成18年度日本植物病理学会大会　2006/06/03

  138. EDS5過剰発現シロイヌナズナにおけるサリチル酸シグナル伝達系の活性化とウイルス抵抗性

    石原岳明, 関根健太郎, 長谷 修, Jyoti Shah, 高橋英樹

    平成18年度日本植物病理学会大会　2006/06/03

  139. キュウリモザイクウイルス抵抗性遺伝子RCY1産物の1アミノ酸置換変異によるウイルス抵抗性の消失と変異部位特異的な過敏感細胞死の抑制

    関根健太郎, 石原岳明, 長谷修, 高橋英樹

    平成18年度日本植物病理学会大会　2006/06/03

  140. ウイルス感染による細胞死とウイルス抵抗性

    高橋英樹

    第3回重点共同利用研究ワークショップ「植物の自己防御と細胞死」　2006/03/27

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  141. 土壌生息菌Pythium oligandrum の細胞壁に存在するエリシチン様エリシターによるシキミ酸経路遺伝子の発現誘導

    高橋 英樹, 石原 岳明, 千葉 彩香, 長谷 修, 竹中 重仁

    平成17年度日本植物病理学会東北部会　2005/09/27

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  142. 生物防除微生物Pythium oligandrum によって誘導されるトマト青枯病抵抗性

    長谷 修, 清水綾乃, 中保一浩, 竹中 重仁, 高橋英樹

    平成17年度日本植物病理学会東北部会　2005/09/27

  143. シロイヌナズナにおけるキュウリモザイクウイルス抵抗性遺伝子RCY1の解析 ―rcy1変異体の新たな系統の単離と相補性試験―

    関根健太郎, 石原岳明, 長谷修, 高橋英樹

    平成17年度日本植物病理学会東北部会　2005/09/27

  144. Molecular mechanism for the resistance to Cucumber mosaic virus in Arabidopsis thaliana International-presentation

    Hideki Takahashi, Ken-Taro Sekine, Takeaki Ishihara, Sayaka Kawakami, Shu Hase

    International Conference of Crop Security (ICCS) 2005　2005/09/20

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  145. Analysis of global gene expression in tomato root treated with elicitin-like glycoprotein elicitor of Pythium oligandrum International-presentation

    Ayano Shimizu, Takeaki Ishihara, Shu Hase, Taneaki Tugane, Daisuke Shibata, Shigehito Takenaka, Hideki Takahashi

    International Conference of Crop Security (ICCS) 2005　2005/09/20

  146. Pythium oligandrum induces transient ethylene production and reduces the severity of bacterial wilt disease caused by Ralstonia solanacearum in tomato International-presentation

    S. Hase, A. Shimizu, K. Nakaho, S. Takenaka, H. Takahashi

    International Conference of Crop Security (ICCS) 2005　2005/09/20

  147. CMV感染に対する防御応答の分子機構

    高橋英樹

    植物ー病原微生物の分子応答機構の解明 ー耐病性植物の創出にむけてー　2005/09/16

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    本人の講演

  148. 植物の防御応答における抵抗性遺伝子とシグナル伝達系の多様性

    高橋英樹

    日本進化学会シンポジウム　2005/08/27

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  149. キュウリモザイクウイルス-シロイヌナズナ系におけるNBS-LRR遺伝子ファミリーの発現・機能解析

    高橋英樹

    平成17年度植物感染生理談話会　2005/08/18

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  150. キュウリモザイクウイルス抵抗性を支配する宿主遺伝子とシグナル伝達機構

    高橋英樹

    岩手大学東北大学ジョイント国際シンポジウム　2005/08

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  151. シロイヌナズナにおいてCMV(Y)抵抗性反応初期に発現量が増加する機能未知遺伝子の解析

    石原岳明, 長谷 修, 池上正人, 高橋英樹

    平成17年度日本植物病理学会大会　2005/03

  152. シロイヌナズナにおけるキュウリモザイクウイルス罹病性変異体の作出と変異体rcy1-2，rcy1-3，rcy1-4の解析

    関根健太郎, 長谷修, 池上正人, 高橋英樹

    平成17年度日本植物病理学会大会　2005/03

  153. 生物防除微生物Pythium oligandrum (PO)の作物根圏への定着性の解明 (1) 土壌中からのPOの定量法の開発とマイクロトムの根圏土壌におけるPOの定着性の解析

    竹中重仁, 関口博之, 高橋英樹, 中保一浩

    平成17年度日本植物病理学会大会　2005/03

  154. 生物防除微生物Pythium oligandrum (PO)およびPOの細胞壁タンパク質エリシターによる誘導抵抗性の分子機構解明 (1) マイクロトムでの誘導抵抗性発現におけるエチレンシグナル伝達系の関与

    長谷 修, 清水綾乃, 竹中重仁, 高橋英樹

    平成17年度日本植物病理学会大会　2005/03

  155. 生物防除微生物Pythium oligandrum (PO)およびPOの細胞壁タンパク質エリシターによる誘導抵抗性の分子機構解明 (2) 細胞壁由来エリシター処理マイクロトムにおける遺伝子発現変動の網羅的解析

    高橋英樹, 長谷 修, 石原岳明, 中保一浩, 柴田大輔, 竹中重仁

    平成17年度日本植物病理学会大会　2005/03

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    本人の発表

  156. DNAアレイを使った実験（植物病理学分野）

    高橋英樹, 長谷 修, 石原岳明, 中保一浩, 柴田大輔, 竹中重仁

    かずさDNA研究所ワークショップ第２回トマトワークショップ　2004/12/02

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  157. ウイルス感染に対する宿主抵抗性

    高橋英樹

    平成16年度植物感染生理談話会　2004/08/19

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    本人の講演

  158. Antagonistic interactions between the SA- and JA- signaling pathways in Arabidopsis modulate expression of defense genes and gene-for-gene resistance to Cucumber mosaic virus. International-presentation

    Takahashi, H, Kanayama, Y, Zheng, M.S, Kusano, T, Hase, S, Ikegami, M, Shah, J

    15th International Conference of Arabidopsis Research　2004/07

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    本人の発表

  159. Enhanced resistance to Cucumber mosaic virus in the Arabidopsis thaliana ssi2 mutant is mediated via an SA-independent mechanism. International-presentation

    Sekine, K.-T, Nandi, A, Ishihara, T, Hase, S, Ikegami, M, Shah, S, Takahashi, H

    15th International Conference of Arabidopsis Research　2004/07

  160. Dissection of signaling pathways for the resistance to Cucumber mosaic virus in Arabidopsis. International-presentation

    Hideki Takahashi

    NIAS-COE/PROBRAIN/TOKUTEI Joint International Symposium, “Plant Immunity” Signaling to acquired resistance　2004/03

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  161. キュウリモザイクウイルス抵抗性シロイヌナズナにおけるNBS-LRR抵抗性遺伝子ファミリーの発現解析

    高橋英樹, 石原岳明, 柴田大輔, 長谷 修, Jyoti Shah

    平成16年度日本植物病理学会大会　2004/03

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  162. Signal transduction pathways governing resistance to Cucumber mosaic virus. International-presentation

    . 9th US-Japan Science Seminar, “Genomics and Genetic Analysis of Plant Parasitism and Defense”　2003/11

  163. Gene expression changes in the resistance response to Cucumber mosaic virus in Arabidopsis thaliana revealed by cDNA macroarray analysis. International-presentation

    9th US-Japan Science Seminar, “Genomics and Genetic Analysis of Plant Parasitism and Defense”　2003/11

  164. キュウリモザイクウイルス抵抗性発現におけるシグナル伝達系の解析(VII): 抵抗性発現におけるPAD4/EDS1遺伝子の関与

    平成15年度日本植物病理学会東北部会　2003/09

  165. キュウリモザイクウイルス感染初期において発現が変動するシロイヌナズナ遺伝子の解析

    平成15年度日本植物病理学会東北部会　2003/09

  166. ) 全身獲得抵抗性が誘導されているシロイヌナズナ変異体のキュウリモザイクウイルスに対する応答

    平成15年度日本植物病理学会東北部会　2003/09

  167. キュウリモザイクウイルス抵抗性発現におけるシグナル伝達系の解析(VI): シロイヌナズナeds5 ein2 coi1トリプル変異体における抵抗性発現

    平成15年度日本植物病理学会大会　2003/04

  168. シロイヌナズナのキュウリモザイクウイルス抵抗性に関与する宿主遺伝子の網羅的解析

    平成15年度日本植物病理学会大会　2003/04

  169. Signaling through RCY1, an Arabidopsis thaliana gene conferring resistance to cucumber mosaic virus International-presentation

    8th International Congress of Plant Pathology　2003/02

  170. ウイルス抵抗性遺伝子は病原菌抵抗性遺伝子と同じローカスに存在する

    科学研究費特定領域研究(A)成果公開シンポジウム「見えてきた耐病性植物作出の戦略」　2002/12/06

  171. キュウリモザイクウイルス外被タンパク質と特異的に結合するプロテインキナーゼの過敏感反応における役割

    平成14年度日本植物病理学会東北部会　2002/09

  172. シロイヌナズナ変異体ssi2-1において誘導されたキュウリモザイクウイルス抵抗性シグナル伝達系の解析

    平成14年度日本植物病理学会東北部会　2002/09

  173. キュウリモザイクウイルス抵抗性発現におけるシグナル伝達機構の解析(V): 抵抗性遺伝子RCY1の単離

    平成14年度日本植物病理学会大会　2002/04

  174. シロイヌナズナ変異体ssi2-1におけるキュウリモザイクウイルス抵抗性の解析

    平成14年度日本植物病理学会大会　2002/04

  175. キュウリモザイクウイルス外被タンパク質と特異的に結合するプロテインキナーゼの病徴発現における役割

    平成14年度日本植物病理学会大会　2002/04

  176. シロイヌナズナのキュウリモザイクウイルス抵抗性発現におけるEDS5の関与

    平成14年度日本植物病理学会東北部会　2002/04

  177. キュウリモザイクウイルス-シロイヌナズナ系におけるウイルス抵抗性反応

    第6回植物ウイルス病研究会「植物ウイルス抵抗性の分子機構」　2002/04

  178. ウイルス感染に対する宿主応答の分子機構

    日本植物生理学会平成14年度シンポジウム　2002/03

  179. キュウリモザイクウイルス変異体によるタバコの過敏感反応の組織細胞学的観察

    平成13年度日本植物病理学会東北部会　2001/10

  180. キュウリモザイクウイルス抵抗性発現におけるシグナル伝達機構の解析(IV): サリチル酸とエチレンを介したシグナル伝達系の役割

    平成13年度日本植物病理学会東北部会　2001/10

  181. 2,5Aase/RNase L発現タバコにおけるウイルス感染に伴う防御関連遺伝子発現の解析

    平成13年度日本植物病理学会東北部会　2001/10

  182. シロイヌナズナのウイルス病抵抗性遺伝子

    特定領域研究「自家不和合性」公開セミナー　2001/07/16

  183. シロイヌナズナのキュウリモザイクウイルス抵抗性遺伝子RCY1の高密度マッピング解析

    平成13年度日本植物病理学会大会　2001/04

  184. キュウリモザイクウイルス抵抗性発現におけるシグナル伝達機構の解析(III)

    平成13年度日本植物病理学会大会　2001/04

  185. キュウリモザイクウイルス黄斑系統の外被タンパク質と相互作用する宿主タンパク質の解析

    平成13年度日本植物病理学会大会　2001/04

  186. キュウリモザイクウイルス抵抗性発現におけるシグナル伝達機構の解析(II): サリチル酸を介したシグナル伝達系変異体における抵抗性発現

    平成12年度日本植物病理学会東北部会　2000/09

  187. キュウリモザイクウイルスに対する宿主応答とシグナル伝達

    平成12年度植物感染生理談話会「植物と微生物相互作用の夜明け」　2000/08

  188. キュウリモザイクウイルスにおける病原性と抵抗性の分子機構

    かずさDNA研究所ワークショップ「ゲノム解析時代の植物病理学の方向」　2000/07/14

  189. キュウリモザイクウイルス抵抗性発現におけるシグナル伝達機構の解析(I)

    平成12年度日本植物病理学会大会　2000/03

  190. アラビドプシスにおけるCMV抵抗性

    第4回植物ウイルス病研究会「植物ウイルス抵抗性の分子生物学」　1998/05/23

  191. 病害抵抗性におけるサリチル酸を介した情報伝達機構

    平成8年度植物感染生理談話会「植物感染生理学研究の現状と将来展望」　1996/07

  192. 病害抵抗性とシグナル伝達

    植物ウイルス研究会「植物ウイルスと関連した宿主因子を探る」　1995/10/30

Show all Show first 5

Research Projects 39

  1. 植物における誘導抵抗性の分子機構解明 Competitive

    System: Grant-in-Aid for Scientific Research

    1997/04 - Present

  2. 有用微生物を用いた病害防除技術の開発 Competitive

    System: Grant-in-Aid for Scientific Research

    1995/05 - Present

  3. Study on Plant-microbe interaction at the molecular level Competitive

    System: Grant-in-Aid for Scientific Research

    1987/04 - Present

  4. Re-evaluation of the significance and function of bacterial viruses in the soil of the terrestrial ecosystem

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2023/06/30 - 2026/03/31

  5. ウイルスの不顕性感染が宿主植物の環境適応能力強化に果たす役割

    高橋 英樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 国際共同研究加速基金(国際共同研究強化(B))

    Category: 国際共同研究加速基金(国際共同研究強化(B))

    Institution: 東北大学

    2022/10/07 - 2026/03/31

  6. 作物の生理障害の機構解明におけるブレークスルーテクノロジーの開発と検証

    金山 喜則, 高橋 英樹, 渡部 敏裕, 須川 成利, 栗原 大輔, 黒田 理人

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(A)

    Institution: 東北大学

    2021/04/05 - 2026/03/31

  7. 比較免疫から読み解くウイルス核酸認識受容体を介した植物免疫システムの分子基盤

    高橋 英樹, 宮下 脩平

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2019/04/01 - 2024/03/31

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    これまで、動物の自然免疫においてウイルス感染防御に主要な役割を果たしている核酸認識受容体を介したウイルス防御システムが、植物免疫にも存在することを示す知見はなかった。しかし最近、poly(I:C)[動物において自然免疫を賦活化させる誘導核酸として広く利用されている]を処理した植物において、防御応答マーカー遺伝子の発現が誘導されるとの報告がなされた。この予備的知見に基づき本研究では、シロイヌナズナに不顕性感染するキュウリモザイクウイルスHo系統[CMV(Ho)]を核酸認識受容体認識ウイルス、Turnip crinkle virus (TCV)をチェレンジ接種ウイルス（TCVの増殖抑制より、ウイルス防御システムが誘導が確認できる）に用いて解析を行った。CMV(Ho)の前接種３日後にTCVをチャレンジ接種したところ、再現性良くTCVの感染増殖と細胞間移行の阻害が認められた。次に、このTCV増殖抑制が、CMV(Ho)の前接種で生成されたCMV核酸分子(1本鎖または2本鎖RNAを想定)の受容体認識によるウイルス防御システム誘導に起因することを調べるため、核酸認識受容体候補である3種類の受容体キナーゼ遺伝子(SERK1, SERK2 and SERK4)にT-DNAを挿入することにより機能を喪失した変異体(serk1, serk2 and serk4変異植物)を作出した。さらに、機能を喪失したserk1, serk2 and serk4変異植物を相互に交配し、複数の受容体キナーゼ遺伝子が機能喪失した二重変異植物を作出した。SERK核酸認識受容体によるCMV核酸分子の認識がウイルス防御システムを誘導するのであれば、serk変異植物へのCMV(Ho)接種では、TCV増殖抑制がキャンセルされることが予想される。次年度は、これらserk変異植物を用いてウイルス防御システムを誘導試験を実施する。

  8. 植物性乳酸菌の自然界における動態解析と乳酸菌定着植物での病害抑制現象の基盤解明

    高橋 英樹, 北澤 春樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2019/06/28 - 2023/03/31

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    本研究では自然界に生息する微生物の中で植物性乳酸菌に注目し、植物性乳酸菌の環境中における循環と、植物性乳酸菌が植物にどのような関係を持っているのかを、植物の病害抑制効果の視点から明らかするにことを目的としている。植物性乳酸菌は動物の自然免疫システムを活性化させて、ヒトや家畜の健康に寄与することが知られているが、その植物性乳酸菌の由来については十分な理解がなされていない。また、植物の茎葉には、植物性乳酸菌が定着しているが、自然界における植物に対する植物性乳酸菌の役割についての理解は不十分である。植物性乳酸菌の環境中における動態と、植物に対する病害抑制効果を評価することにより、環境中における植物性乳酸菌と植物・動物との相互関係を包括的理解しようとするものであり、植物の病害防除への応用も期待される。本年度は、イネ種子を滅菌蒸留水に浸漬処理（発芽のための吸水処理）中にイネ種子より滲出してくる液体を乳酸菌選抜培地で培養し、植物性乳酸菌候補となるコロニーを単離した。同菌の分類同定とイネ植物体への定着およびイネ苗病害抑制効果についての検討が必要であるが、コロナ禍の影響により十分な実験時間が確保できなかったため、次年度にはそれらの解析を早急に実施する。一方、植物性乳酸菌であるLactobacillus plantarum CRL1506は、腸管上皮細胞において核酸認識免疫受容体TLR3を介した自然免疫応答を誘導し、その誘導にはlipoteichoic acidの関与が確認された。

  9. 「ネオウイルス学」の国際活動支援

    河岡 義裕, 朝長 啓造, 澤 洋文, 高橋 英樹, 川口 寧, 渡辺 登喜子, 松浦 善治, 鈴木 信弘, 長崎 慶三

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東京大学

    2016/06/30 - 2023/03/31

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    国際活動支援班では、各研究班の海外における研究活動を促進するために、 (1) 国際研究ネットワークの整備および強化、(2) 円滑な海外での研究試料の採取支援・提供、(3) 国際シンポジウムの開催および海外研究者の招聘による人的連携の促進、(4) サンプル収集のための海外への研究者の派遣の支援、(5) 最先端研究を行う先進諸国への若手研究者の中長期的派遣の支援、(6) 国際研究者ネットワークへの積極的な情報発信を行なっている。 2021年度は、国内で国際学会を1回 [第19回あわじ感染と免疫国際フォーラム]を共催し、また、同学会の直後に国際シンポジウムを主催した。これらの学会およびシンポジウムを通じて、海外から総計12名の研究者をオンライン招聘したほか、班員12名が発表した。招聘した研究者と計画研究班、および公募研究班班員はオンライン交流を通じて人的連携を構築した。また、引き続き、本領域のホームページの英語版を通して国際社会に本領域の活動を発信している。 COVID-19の感染拡大により、海外へサンプル収集に行くことが困難な状況であったため、海外からのサンプル輸入、および海外で採取したサンプルのネオウイルス学的解析、国内で採取したサンプルの解析結果との比較解析のための支援を実施した。

  10. ネオウイルス学：生命の源流から超個体、そしてエコ・スフィアーへ

    河岡 義裕, 朝長 啓造, 澤 洋文, 松浦 善治, 川口 寧, 渡辺 登喜子, 鈴木 信弘, 高橋 英樹, 長崎 慶三

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東京大学

    2021/04/01 - 2022/03/31

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    「ネオウイルス学」領域内の計画研究および公募研究で得られた各班それぞれの研究テーマについて研究成果を取りまとめ、さらに班間、あるいは、「共進化」、「共生」、「多様性」ユニット間の連携テーマの重要性・意義を考慮しつつ、領域全体の研究成果を取りまとめた。業績集を作成し、冊子体として印刷して領域内外で配布することによって、本領域の研究成果を発信した。報告内容は、出来るだけ分かりやすい記述を心がけ、学術分野だけでなく、一般国民にも読んでもらえるような業績集を作成した。また、高校生、大学生、一般の国民に向けた講演会やセミナー、そして領域ホームページとTwitterによって、領域の活動内容や研究成果を報告することによって、国民に向けた研究成果の発信に努めた。また、新しい試みとして「ウイルスおりがみ」を作成した。ウイルスおりがみは、おりがみを折ることで、一般国民にウイルスの面白さを知ってもらうことを目的とした、科学コミュニケーションツールであり、公募研究班の牧野晶子助教（京都大）、デザイン・ユニットCOCHAE、合同会社SHIBUYA PUBLISHING & BOOKSELLERS、三宅文子アート・プランナーらのグループによって作成された。ウイルスおりがみには領域内の研究者による解説も加えられており、ウイルスの基本、構造の面白さ、役に立つウイルス、生き物の中にいる多様なウイルスについて学ぶことができる。ウイルスおりがみの配布の公募をしたところ2288部の応募があり、抽選で500部を無償で配布した。日本デザイン振興会が主催するグッドデザイン賞へウイルスおりがみを出品中である。

  11. Basic analysis of environmental stress tolerance to plants and maintenance of ecosystem homeostasis due to persistent virus infection

    TAKAHASHI Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: Tohoku University

    2016/06/30 - 2021/03/31

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    In recent years, it has become clear that various plants in the natural world have a base sequence homologous to the viral genome on the genome and that the virus is persistently infected. In this study, we elucidated the role and specific molecular basis of viruses that infect plants without showing clear symptoms in their vital activities. As a result, it was shown that the cytosine methylation/demethylation level of the promoter region of the host gene contributes to resistance to environmental stress through fluctuations due to persistent infection of the virus. Furthermore, it was clarified that the expression of the viral genome existing in the host genome changes the flower pattern. These findings provide new insights into the role of viruses in plant life activities.

  12. Neo-virology: the raison d'etre of viruses

    Kawoaka Yoshihiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: The University of Tokyo

    2016/06/30 - 2021/03/31

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    We have established a new academic field, designated as ‘Neo-virology’, in which we define a virus as a component of the global ecosystem and aim to elucidate its key roles in host organisms and the global ecosystem. We have constructed a system to support research activities, facilitate collaborations among research groups, develop human resources, plan and manage scientific meetings, and support public relations activities, all of which have resulted in accelerating this new field of research. Our efforts have produced numerous noteworthy publications and have helped develop many young investigators, who will continue the growth of Neo-Virology into the future.

  13. An integrated approach for elucidation of the mechanism of blossom-end rot occurrence and overcoming it

    Kanayama Yoshinori

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2016/04/01 - 2021/03/31

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    Blossom end rot in tomato fruit is a physiological disorder that causes enormous damage in the world and Japan, and its mechanism of occurrence has not been fully elucidated. Therefore, in this project, a genetic approach using chromosomal fragment substitution lines that stably show resistance to blossom end rot was performed with ionomic analysis of all ions containing calcium and analysis to clarify the dynamics of calcium. Furthermore, transcriptome analysis and the regulation of gene expression were performed to obtain the evidence. Through these approaches, we conducted basic research aimed at elucidating the mechanism of occurrence of blossom end rot and providing breeding materials for overcoming it.

  14. Molecular basis of activation of plant immune system by atomasphic plasma irradiation and its application to plant disease control

    Takahashi Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2016/04/01 - 2020/03/31

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    When rice seeds were irradiated with atmospheric plasma, the occurrence of "Bakanae disease" and bacterial damping-off disease in rice seedlings was suppressed. Irradiation of Arabidopsis thaliana with plasma reduced the level of virus multiplication. It has been known that reactive oxygen species act as a signaling molecule for plant immune system. Interestingly, the comprehensive analysis of reactive molecules produced by atmospheric plasma irradiation indicated that peroxynitrite (HOONO) and pernitric acid (HOONO2), which have antimicrobial activity, and N2O5 accumulated in gas phase, and NO3- was detected in liquid phase. Furthermore, in plants irradiated with atmospheric plasma, defense-related gene expression: PR1, PDF1.2 and ChiB, was significantly induced, suggesting that plant immune system seems to be activated by irradiation of plants with atmospheric plasma.

  15. Improvement of biocontrol activity in industrial-scale composters and screening of microorganisms showing biocontrol activities from the composters

    Abe Keietsu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2016/07/19 - 2019/03/31

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    Burkholderia glumae causes seedling rot of rice. We revealed that the compost from cow manure produced by a field-scale composter protects the rice from the infection. Additionally, we isolated Chryseobacterium sp. ON_d1 from the compost, which has strong biocontrol activity against the infection. The genome analysis of the strain indicates that it belongs to a novel species within the genus Chryseobacterium and includes N-Acylhomoserine Lactonase gene and Siderophore biosynthesis gene cluster that may be involved in biocontrol activity. We also found that other relative type strains have the same biocontrol activity against the infection, indicating that the taxonomic group has the beneficial function in common.

  16. Molecular basis of immune receptor conferring dual resistance to virus and oomycete and its application for disease control

    Takahashi Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2019/03/31

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    RCY1/HRT/RPP8 locus of Arabidopsis thaliana encodes NB-LRR class R-protein conferring the resistance to CMV, TCV and Hpa. The expression of RCY1/HRT/RPP8 locus was regulated by epigenetic modification through cytosine methylation of its promoter region, and accumulated level of R-protein was correlated with degree of disease resistance to pathogens. RCY1/HRT/RPP8 R-protein can recognize the molecular structure of multiple complex of CP mediated by N-terminal α-helix domain of CP.

  17. Molecular basis of rice defense against the bacterial leaf blight disease via control of glutamate synthesis in the leaf vasculature

    HAYAKAWA Toshihiko, TAKAHASHI Hideki, KOYAMA Hiroyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2017/03/31

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    Nitrogen nutrition is an essential macronutrient required in quantity for plant growth and productivity. The regulation of glutamate biosynthesis via glutamine-responsive gene expression is important for nitrogen use in rice. It has also been suggested that glutamate biosynthesis in rice may be linked to the pathogenesis of the bacterial leaf blight disease. Results of this study suggested that the ACT-domain repeat protein 9 (OsACR9), which is composed of domains having homology with the glutamine-binding ACT domains of bacterial glutamine sensors, is associated with glutamine-responsive gene expression of the enzyme related to glutamate biosynthesis and important for nitrogen and carbon metabolisms in rice.

  18. Acceleration of functional genomics by virus-induced gene silencing

    Kanayama Yoshinori, TAO Ryutaro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2012/04/01 - 2017/03/31

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    Accumulation of genome-related information is advanced in horticultural crops; however, its contribution to horticultural production is not necessarily high. One of the main reasons is that the functional genomics method to prove the function of a gene has not been established in the field of horticulture including various species. Therefore, the purpose of this study was to accelerate the identification of the function of useful genes by applying virus-induced gene silencing to horticultural crops. In this study, we analyzed the physiological role and horticultural importance of genes to be controlled in fruit trees, vegetables, and flowers. In addition, we tried to develop viral vectors applicable to various horticultural crops, and to examine the mechanism of infection related to the use of cucumber mosaic virus with potentially general versatility because of wide host range.

  19. Challenge to plant immunity control by plant activator using plasma electrification droplet

    KANEKO TOSHIRO, TAKAHASHI HIDEKI, KATO TOSHIAKI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2014/04/01 - 2016/03/31

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    Two functions of the plasma generated reactive species for agricultural applications, the germination suppression of pathogenic conidia and the induction of defense related genes of rice seeds, are experimentally investigated. The germination suppression effect is enhanced by the water molecular fraction increase in plasma, correlating with hydroxy radical density in the plasma effluent and nitrate ion concentration in the conidia suspension. On the other hand, the gene expression degree of the defense related genes of rice seeds are significantly increased under a certain plasma effluent exposure condition and are varied simultaneously with the mixture variation of the reactive species. The difference of the reactive species correlated with the germination suppression and the defense related genes indicates the feasibility to switch those functions by the reactive species control in the plasma effluent.

  20. Analysis of metagenome and metaranscriptome of endophytic microorganisms in plants and its application for disease control

    Takahashi Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2012/04/01 - 2016/03/31

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    Organic rice farming system has been successfully established in a number of local regions in Japan. The outbreak of plant diseases is effectively suppressed in the organic farming system. The features common to nine organic farmed soils used for rice nursery cultivation, which were supplied by nine independent organic rice farmers, were analyzed. The emergence of bacterial seedling diseases was equally suppressed in nine organic farmed soils, but not two conventional soils. On physicochemical analysis of those soils, there was not either any component common to nine organic soils or difference between organic soils and control conventional soils. On the other hands, bacterial and fungal microbiome analysis indicated that their populations in nine organic soils were more diverse than those in conventional soils. The diverse of microbiome may play an indispensable role for suppressing rice seedling diseases in organic agriculture.

  21. Study on the mechanism of dual resistance to virus and oomycete using their chimeric resistance genes

    TAKAHASHI Hideki, ANDO Sugihiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2010/04/01 - 2015/03/31

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    RCY1 confers the hypersensitive resistance (HR) response to Cucumber mosaic virus [CMV(Y)] in A. thaliana, whereas its allelic RPP8 does the resistance to H. arabidopsidis. When RCY1, RPP8 and a series of chimeric constructs between RCY1 and RPP8 were transiently expressed in N. benthamiana, respectively, HR cell death was induced in the leaf tissue expressing chimeric construct carrying CC-NB of RPP8 and LRR of RCY1, suggesting that LRR domain of RCY1 determines the recognition of CMV(Y). Furthermore, to study on the regulatory mechanism of RCY1/RPP8 gene expression, we analyzed the contribution of each structural element of the gene. As the result, we can find that the presence of intron in RCY1/RPP8 genes is indispensable for higher level of RCY1/RPP8 protein accumulation. Those findings regarding pathogen recognition specificity and the role of intron for the resistance to pathogens will give new insight to understand the plant immune system against pathogen infection.

  22. Metagenomic analysis of microbial population in intercellular fluid of rice and its application for disease control

    TAKAHASHI Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2009 - 2011

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    Microorganisms inhabiting in intercellular spaces of plants may stimulate plant defense systems or promote plant growth, whereas they are likely to benefit from the exudates of plant cells. In this study, we analyzed the structure of overall community of endophytic microorganisms in plants and their disease suppressive activity. Intercellular fluid (IF)was collected from leaf blades and sheaths of rice.(1)Denaturing gradient gel electrophoresis (DGGE)pattern and nucleotide sequence analyses of 16S and 18S_rDNA fragments amplified from IF DNA seemed to be available to understand global microbial ecology in plants.(2)Pseudomonas sp., bcillus sp., Curtobacterium sp. and Acinetobacter sp. were preferentially isolated as endophytic bacteria from rice IF cultivated by organic farming system.(3)Some of those endophytic bacteria exhibited potential activity to suppress rice blast and rice bacterial rot seedling diseases.

  23. Application of virus-induced gene silencing to horticultural crops

    KANAYAMA Yoshinori, TAKAHASHI Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2009 - 2011

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    Contribution of genome-related research to horticultural production is not high in spite of its remarkable progress in recent because method of functional genomics has not been established in horticultural crops including so many species. This study was carried out for application of virus-induced gene silencing, which can be useful for a wide range of species, to horticultural crops and useful information was obtained on technology development and genes for functional analysis using the technique.

  24. Functional analysis of molecular complex composed by CMV-resistance protein RCY1 and other host proteins

    TAKAHASHI Hideki, YOSHIKAWA Nobuyuki, HASE Shu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2007 - 2009

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    Resistance gene to CMV(Y), RCY1, encodes CC-NBS-LRR-containing protein. In this study, we analyzed the molecular aspect of a complex composed by RCY1 and other host proteins. In conclusion, (1) RCY1-conferred resistance to CMV(Y) required three structural domains : CC, NBS and LRR in RCY1 protein, (2) HR-cell death was induced by Agrobacterium-mediated transient expression of RCY1, but co-expression of all combination of CC, NBS and LRR domain proteins in plant cell did not induce not only the cell death but also molecular interaction among them, indicating that three domains are not able to act trans-complimentarily, (3) A protein with molecular weight of 43kDa and a NTPase containing 'GHKL'ATPase motif were identified as a RCY1-interacting proteins. These finding will give a new insight for understanding the function of RCY1 protein conferring the resistance to CMV(Y).

  25. Studies of tomato chromosome 8 using chromosome segment substitution lines from wild tomato species

    KANAYAMA Yoshinori, TAKAHASHI Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2006 - 2008

  26. Functional analysis of NB-LRR gene family for plant immunity

    TAKAHASHI Hideki, HASE Shu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2004 - 2006

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    We have isolated Cucumber mosaic virus (CMV) resistance gene RCY1 encoding coiled coil (CC), nucleotide binding site (NB) and leucine-rich repeat (LRR) domain-containing protein. Arabidopsis genome contains 〜200 member of RCY1 paralogs encoding NB-LRR protein. In this project, the function of RCY1 and other NB-LRR genes for the resistance to CMV was analyzed using a serious of RCY1 mutants and NB-LRR custom array. The obtained results suggested that (1)all CC, NB and LRR domains are required for complete resistance to CMV, (2)the resistance to CMV is dependent on the level of RCY1 expression, (3)RCY1 separately regulates development of hypersensitive cell death and restriction of virus spread. Furthermore, global analysis of 〜200 members of NB-LRR gene family in CMV resistance using custom array indicated that expression of five NB-LRR genes : At1g72890,At1g72900,At1g72910,At1g72930,At1g72940, which tandemly located on the chromosome I, was up-regulated in CMV resistance. The CMV-inducible R-like gene cluster was named "CIR1". To further analyze the function of CIR1 for CMV resistance, we transformed CIR1 cDNA under the control of CaMV35S promoter into A. thaliana carrying RCY1. The response of the obtained transformants to CMV will be extensively analyzed. The information obtained from this research will give a new insight to study the role of NB-LRR gene family for CMV resistance and molecular basis of virus resistance in plants.

  27. Functional genomics of the model horticultural crop, Micro-Tom.

    KANAYAMA Yoshinori, TAKAHASHI Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2003 - 2005

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    Functional genomics is lately developing because all DNA sequences of the genome have been clarified in Arabidopsis thaliana that is the model plant. On the other hand, functional genomics in horticultural crops is not easy because there are so many species involved in horticultural crops. Therefore, an advanced research by using some model horticultural crops is needed. Arabidopsis thaliana is not useful as the model of the fruit trees and the fruit vegetables. Tomato is not only commercially important but also useful as a model horticultural plant because its transformation is comparatively easy ; its physiological information is abundant ; there is expressed sequence tag (EST) containing more than 100,000 sequences. However, there are some problems, which are the large plant size and the long generation time. Micro-Tom is a miniature tomato that is almost genetically identical to the cultivation tomatoes. Micro-Tom could be a good model plant because of its small size (5-10 cm of plant height), short generation time, and high transformation efficiency. Thus, Micro-Tom was used in this experiment as a model horticultural crop for molecular genetics. A high degree of availability of Micro-Tom as a model experimental plant was confirmed through the analysis of proton pump, pathogen response, etc. in this research. Moreover, the research on various horticultural crops such as the fruit vegetables and fruit trees was also carried out. In the future, the information and the technique of functional genomic developed using Micro-Tom are expected to be applied widely in various horticultural crops.

  28. Mechanism of signal transduction pathways for the host response to virus infection

    TAKAHASHI Hideki, OHASHI Yuko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Priority Areas

    Institution: TOHOKU UNIVERSITY

    2000 - 2005

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    Cucumber mosaic virus (CMV) and Tobacco mosaic virus (TMV) are the best-characterized positive-sense single-stranded RNA viruses. In this project, the signal transduction pathways for the resistance to CMV and TMV were studied. CMV-Arabidopsis system: A. thaliana ecotype C24 is resistant to a yellow strain of CMV [CMV(Y)]. This resistance to CMV(Y) was conferred by a single dominant gene, RCY1, which is a member of RPP8/HRT resistance gene family. Our analysis of Arabidopsis mutants deficient in SA, JA and ethylene signaling pathways indicated that an SA-and ethylene-dependent signaling pathway played an important role in the expression of the high level of resistance to CMV(Y) conferred by the RCY1 gene, However, the combined impairment of SA and ethylene signaling in the nahG etrl RCY1 double mutant did not completely abolish the RCY1-conferred resistance to CMV(Y). We therefore proposed that in addition to SA and ethylene signaling, a novel uncharacterized signaling pathway was required for the full resistance to CMV(Y) conferred by the RCY1 gene. Moreover, cross-talk between the SA and JA signaling pathways is an important modulator of RCY1-conferred resistance to CMV(Y). The analysis of double Arabidopsis mutants indicated that a CO/1-dependent mechanism negatively modulated the RCY1-mediated activation of SA signaling pathway and resistance to CMV(Y). The gene-for-gene resistance to Turnip mosaic virus (TCV) conferred by HRT of ecotype Di-17 was dependent on the SA-mediated signaling pathway, but not on the JA and ethylene signaling pathways. Although RCY1 in ecotype C24 was an ortholog of HRT in the ecotype Di-17, the extent of the requirement for SA signaling in conferring resistance to CMV(Y) and TCV was different between RCY1-and HRT-containing plants. The difference in the requirement of defense mechanisms targeting these two viruses is further exemplified by studies with the Arabidopsis ssi2 mutant. While the ssi2 mutant exhibited enhanced resistance to CMV(Y), it did not confer enhanced resistance to TCV. In summary our results suggest that the Arabidopsis RCYI and HRT genes evolved to recruit different combinations of signal transduction pathways for conferring resistance to two different viruses. TMV-Tobacco system: The, signaling pathway for acquired resistance to pathogen infection has been examined using a synchronous HR cell-death-inducing system in TMV-infected tobacco plants carrying the temperature-sensitive N resistance gene, as well as the wound-signaling pathway which cross-talks with HR-signaling. We studied the function of genes whose expression were induced or repressed 3 h after HR triggering. WRK: A receptor-like protein kinase gene was induced rapidly after HR triggering and wounding. WRK was constitutively expressed at a low level, and induced by a simple temperature transfer from 30'C to 20'C independent of the N gene. In transgenic tobacco with suppressed WRK expression, wound-induced phenomena such as activation of defense-related MAPKS (WIPK and SIPK), JA accumulation, and expression of wound-inducible genes such as PI-II were inhibited, suggesting involvement of WRK at least in wound-signaling. HSP90 : In transgenic tobacco plants with suppressed HSP90 gene expression, the level of HR-induced electrolytic leakage, an indicator of cell death, was considerably inhibited with attenuated resistance to TMV. Treatment with geldanamycin, a specific inhibitor of Hsp90, reduced the ion leakage and wound-induced activation of WIPK and SIPK, indicating the involvement of HSP90 in HR-signaling. CaM (calmodulin) : We isolated 13 tobacco genes encoding three plant-specific types of CaMs, some of which were induced by HR-triggering or wounding. The amount of each type CaM dynamically changed after HR or wounding, Tobacco CaMs activates target enzymes specifically in a Ca^<2+>dependent and type-specific manner. As a new CaM-binding protein, a MAPK phosphatase (MKP1) was isolated. The gene product inactivated SIPK in vitro. In MKP1 overexpressors, wound-induced activation of WIPK and SIPK was inhibited, suggesting Ca^2/CaM signal functions upstream in MAPK-cascade at least in wound-signaling. WAF-1: WIPK is activated enzymatically in response to wounding and HR by TMV infection. (11E,13E)-labda-11,13-diene-8a,15-diol, designated WAF-1 was identified as WIPK-activating substance from TMV-infected tobacco leaves. WAF-1, a novel diterpene, functions as an endogenous signal mediating defense responses of tobacco plants to TMV infection and wounding.

  29. Molecular Mechanisms of Plant-Pathogenic Microbe Interaction-Toward Production of Disease Resistant Plants-

    UYEDA Ichiro, MASUTA Chikara, SHIRAISHI Tomonori, HIBI Tadaaki, DOKE Nooriyuki, MAYAMA Shigeyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Priority Areas

    Institution: Hokkaido University

    1999 - 2005

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    Studies are undertaken to elucidate the following ; 1) molecular mechanisms involved in various plant defense responses through the signal transduction, 2) interactions between pathogenic microbes and host factors involved in pathogenicity and host resistance mechanisms. During the studies, research meetings, seminars for the young scientists and symposia were organized and 16 newsletters have been published. Major results are summarized as below : 1) It was found that viral RNAi suppressor activity is closely linked with the virus genome replication. The study pioneered a new aspect of RNA silencing. 2) Hypersensitive cell death of plants was found to be genetically programmed and proposed there are common mechanisms among animals and plants that execute cell death. 3) Cross talk between SA and JA signal transductions are shown during defense response to viral infection. 4) Transgenic potato plant that is resistant to P. infestance was generated. The defense response is engineered to express only when pathogen attacks the plants but not when wound is made. 5) Cell wall was proved to be the first to trigger defense response and critical factors involved have been isolated. 6) Can-channel was shown to play a critical role in systemic acquired resistance. 7) One of molecular mechanisms how a host specific toxin acts in a susceptible host is elucidated at a molecular level.

  30. 病害抵抗性遺伝子の病原体認識ドメインの解析とその改変による病害防除への応用

    高橋 英樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 萌芽研究

    Institution: 東北大学

    2003 - 2004

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    シロイヌナズナaccession C24は,単一優性であるCC-NBS-LRRタイプの抵抗性遺伝子RCY1を持ち,キュウリモザイクウイルス黄斑系統(CMV-Y)に対して過敏感反応(HR)を示す.本研究課題では,C24にEMSを用いて突然変異を誘起することによりCMV罹病性変異体の作出を試みた.M2植物14,744個体中36個体で,CMV-Yを接種した葉にHRが起こらずウイルスの全身感染が認められた.さらにその後代のM3植物においてもウイルスの全身感染が認められた4系統(#250-037,#219-053,#219-066,#212-001)を目的のCMV罹病性変異体とし原因遺伝子の同定を行った.遺伝分析とマッピングの結果,全て劣性変異で,原因遺伝子がRCY1近傍に存在することが示された.さらにRCY1を持たないaccession Colと各変異体を交配して得られた植物の後代が全てCMV-Tに対して罹病性であったことから,RCY1自体が変異している可能性が示された.各々のRCY1領域をシークエンス解析したところ,いずれも一塩基の変異によるアミノ酸置換がCC又はLRRモチーフに生じている変異体であった.RCY1とその対立遺伝子であるTurnip crinkle virus (TCV)抵抗性遺伝子HRTとの比較から、特にLRRドメイン内でアミノ酸が変化していることが明らかになっているが、本研究結果はLRRドメインが病原体認識に重要な役割を果たしていることを強く示している。

  31. 植物-病原微生物の分子応答と耐病性の分子機構の解明

    上田 一郎, 日比 忠明, 高橋 英樹, 古澤 巌, 道家 紀志, 白石 友紀

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 北海道大学

    1998 - 1998

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    植物が病原微生物の侵入に応答して、耐病性を示す現象を分子レベルで解明することは、病気を制御する新戦略を構築するために今後益々重要であることを確認した。このため、本企画調査では、耐病性の分子機構に関して病理学、遺伝学、分子生物学、生化学、生理学の個々の分野で卓越した貢献をしている日本の研究者が、それぞれの専門領域にとどまらず、情報交換と討論の中で、今後の研究の明確な方向付けを行い、研究協力体制を整備して将来重点領域として発展させる為の準備段階とした. 最近、分子生物学・分子遺伝学的手法を用いて耐病性の分子機構を解明する研究が世界的に急速に成果を上げてきており、永年の生化学的、生理学的な植物の防御機構に関する解析の蓄積と相まって、現在は断片的であるが、近い将来には、耐病性機構が具体的かつ統一的に解明されるであろうと思われる.そこで、合同班会議などを開催し、研究者それぞれに行っている研究をお互いに紹介して学会発表などよりも深く議論をしてゆくなかで、どこが重要な問題点か、欧米の研究の追試ではなくオリジナルな点はどこか、どこに注目すれば国際的にも重要な研究が確立されていけるかなどを論議した。 またその重要性にもかかわらず、この分野に携わる研究者の人口はかなり少ない。研究の底辺を広げていくことも重要であろう。そのために、まず、研究分担者が情報を共有する目的で本プロジェクトに関係する人々を集めたシンポジウムを企画した。 ‘植物-徹生物の分子応答の解明にむけて'を開催し、アメリカより招へいした2名を含む7名の研究者による講演と討論を実施した。 以上の、討論とシンポジウムの成果を踏まえて、科学研究費補助金「特定領域研究(A)」平成11年度発足特定領域申請書‘植物-病原微生物の分子応答機構の解明-耐病性植物の創出に向けて-'を作成して申請した。

  32. 遺伝子タギング法によるウイルス病抵抗性遺伝子と抵抗性発現調節遺伝子の単離と解析

    高橋 英樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 奨励研究(A)

    Institution: 東京農工大学

    1996 - 1996

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    キュウリモザイクウイルス系統(CMV-Y)をアラビドプシスecotype C24に接種すると過敏感反応を、ecotype Col.に接種すると全身感染を示す。これまでの研究よりこの過敏感反応は、C24の有する単一優性遺伝子(RCY1)とCMV-YのRNA3に存在する非病原性遺伝子との組合せにより誘導されることが明らかになっている。本研究では、遺伝子タギング法によりRCY1を単離するために、RCY1の遺伝子マッピングとC24へのトランスポゾンの導入を行った。(1)これまでに遺伝子地図上にマップされている遺伝的マーカーとSSLPマーカーを用いてArabidopsis thaliana C24染色体上におけるRCY1遺伝子の位置を解析したところ、第5染色体のマーカーnga139とnga106の間に存在することが明らかになった。(2)C24を、トウモロコシのトランスポゾンAcを導入したアラビドプシス【.right filled triangle.】Nae IsAc(Gus)-1(Arabidopsis Biological Resource Center,Ohio,U.S.A.)と交配し、transposase遺伝子をC24に導入した。(3)C24をトウモロコシのトランスポゾンDsを導入したアラビドプシスHmR Ds-A3(Arabidopsis Biological Resource Center,Ohio,U.S.A)と交配し、DsエレメントをC24に導入した。(4)交配により得られた両植物をかけ合わせ種子を得た。この種子細胞にはAcとDsが共存するため、Dsに転移が誘導される。これからは、得られた約10,000種子について、CMV-Yに対する反応を調べ、RCY1遺伝子がDsの挿入を受けて不活性化されCMV-Yが全身感染するようになった個体(RCY1がトランスポゾンタギングされた個体)を選抜する予定である。

  33. Isotation and analysis of symptom expression genes in virus-infected by transposon-tagging method.

    TAKAHASHI Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for General Scientific Research (C)

    Institution: Tohoku University

    1992 - 1993

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    To investigate the genetic determinants of severe chlorotic spot symptoms in cucumber mosaic virus(CMV[Y])-inoculated tobacco(Nicotiana tabacum'Ky57')leaves, symptom expression was compared among tobaccos(N.tabacum'Ky57', N.tabacum'Xanthi nc', and their hybrids)inoculated with CMV(Y), CMV(O), or pseudorecombinants of both strains of CMV.Xanthi nc showed very mild chlorotic spot shmptoms in CMV(Y)-inoculated leaves. Both CMV(O)-inoculated Ky57 and Xanthi nc leaves showed no symptoms. In pseudorecombinant-inoculated Ky57 leaves, severe symptoms were determined by chimeric CMV(O)and CMV(Y)RNA3 transcripts, which contained most of the coat protein gene of CMV(Y). Genetic analysis of severe sympotom formation in CMV(Y). or pseudorecombinantinoculated tobaccos indicated that two recessive nuclearcoded host genes determined severe symptom formation in CMV(Y)-inoculated Ky57. The two recessive host genetic loci completely cosegregated with the two recessive burley loci(yb1 and yb2 genes)of Ky57, whose character is an apparent reduction in chlorophyll of the stems. When several cultivars of N.tabacum were inoculated with CMV(Y), all burley tobaccos clearly showed severe symptoms, but most other cultivars showed very mild symptoms, These results suggest that severe chlorotic spot symptom formation in CMV(Y)-inoculated Ky57 is induced by a combination of the virus coat protein gene and two recessive host genes that are closely linked to or identical to yb genes.

  34. Studies on the control of signaling molecules expressed in hairpin-loop linear DNA plasmid in the plant pathogenic fungus

    HASHIDA Teruyoshi, KARASAWA Akira, TAKAHASHI Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for General Scientific Research (B)

    Institution: TOHOKU UNIVERSITY

    1991 - 1993

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    The plant pathogenic isolate RI-64 of anastomosis group 4 of Rhizoctonia solani possesses three linear DNA plasmids (pRS64-1, -2, and -3). Unique poly(A)^-RNA of 0.5 kb hybridizable with the pRS64 DNAs was found in mycelial cells of the isolate RI-64. The overall homology at the nucleotide level between pRS64-1, -2, and -3 and the cDNA prepared for the poly(A)^-RNA was 100%, 73%, and 84%, respectively. The open reading frames found in pRS64-1, -2, and -3 (ORF1-1, ORF2-1, and ORF3-1) were 68 amino acids long. The amino acid sequence showed no significant homology with known properties. Extracts from E.coli cells expressing ORF1-1 gave a specific protein of 7 kDa. Antisera raised to the ORF1-1 product obtained from E.Coli cells cross reacted with the specific proteins found in the mycelia. The results indicate that the DNA plasmids found in the mycella. The results indicate that the DNA plasmids found in R.solani contain a sequence for a specific protein, that may be involved in determination of pathogenicity to plants.

  35. TーDNAtagging法によるウイルス感染植物病徴発現遺伝子の単離と構造解析

    高橋 英樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 一般研究(C)

    Institution: 東北大学

    1991 - 1991

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    キュウリモザイクウイルスY系統「CMY(Y)」接種タバコ葉において病徴発現に関与するタンパク質として、光合成の光化学系IIを構成する22,23kdタンパク質を既に同定している。今年は、これら2種のタンパク質が変動した結果、病徴発現に伴い光合成活性が低下するかどうか検討を行った。チラコイド膜を精製し、DCIPを電子受容体、H_2Oを電子供与体として、電子伝達活性を測定したところ、健全葉および、無病徴感染を示すCMV(O)接種葉にたいして、CMY(Y)接種葉では約50%の活性低下が認められた。しかし、Tris処理により光化学系IIの中の酸素発生系を特異的に阻害した状態で、DPCを電子供与体として、電子伝達活性を測定したところ、健全葉、CMY(O)、CMY(Y)接種葉いずれにおいてもほとんど差は認められなかった。従って、CMY(Y)接種タバコ葉において認められた電子伝達活性の低下は、酸素発生活性の低下に起因するものと考えられる。酸素発生系の他の構成タンパク質である33kdと18kdタンパク質の変動についても検討したが、いずれも病徴発現に伴い低下することはなく、病徴発現後に初めて低下が認められた。以上の結果より、CMY(Y)接種タバコ葉における病徴発沼現の一因として、葉緑体チラコイド膜の光化学系II・酸素発生系を構成する22、23kdタンパク質の低下により、酸素発生活性が阻害され、光合成低下と葉緑体機能の低下するプロセスがあるものと考えられた。今後、Gene tagging法により、この22、23kdタンパク質遺伝子の発現を調節している遺伝子群の単離を行う予定である。

  36. Gene tagging法による植物ウイルス抵抗性遺伝子の単離と機能解析

    高橋 英樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 奨励研究(A)

    Institution: 東北大学

    1990 - 1990

  37. Structural and Functional Analysis of Linear Plasmid DNAs of Plant Pathogenic Fungus

    HAYASHIDA Teruyoshi, TAKAHASHI Hideki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for General Scientific Research (B)

    Institution: Tohoku University

    1988 - 1990

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    1. Plasmid-like DNA (plDNA) was found in 48 out of 114 field isolates of Rhizoctonia solani. These 48 isolates were distributed as follows among the nine anastomosis groups (AG) and intraspecific groups (ISG) recognized among Japanese isolates of R. solani : 0 in AG-1 (sasakii type), 1 in AG-1 (web-blight type), 0 in AG-2-1, 11 in AG-2-2 (rush type), 10 in AG-2-2 (root rot type), 10 in AG-3, 8 in AG-4, 4 in AG-5 and 4 in AG-6. Each isolate carried one, two or three plDNAs identified by gel electrophoresis. Electron microscopic analysis revealed that all these plDNAs were linear molecules. The sequence homology among plDNAs found in representative isolates was examined by Southern blot analysis, using nick-translated plDANs as probes. Considerable sequence homology was observed among plDNAs obtained from isolates within the same AG and ISG. The plDNAs occurring in the isolates of AG-2-2 were classified into two groups on the basis of the sequence homology. 2. Three linear DNA plasmids were found in isolate RI-64 of anastomosis group 4 (AG-4) of Rhizoctonia solani. These plasmids, designated pRS64-1, -2, and -3, possessed the same size of 2.7 kb. Restriction mapping and Southern hybridization anlysis of pRS64-1, -2, and -3 revealed the presence of homologous regions at both termini. The plasmid DNAs were resistant to both 3'-exonuclease and 5'-exonuclease even after treatment with protease K or alkali. The length of both terminal fragments that were generated by restriction endonuclease digestion was doubled under the denaturation condition, indicating that the linear plasmid DNAs have hairpin loops at both termini. Southern blotting analysis of total DNA showed the presence of two types of dimeric forms of pRS64 DNA. One is a head-to-head dimer and the other is a tail-to-tail dimer. The role of these unique DNA structures in replication of th

  38. Gene tagging法によるウイルス感染植物病徴発現遺伝子の単離と機能解析

    高橋 英樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 奨励研究(A)

    Institution: 東北大学

    1989 - 1989

  39. 植物DNAウイルス遺伝子の転写調節に関する研究

    高橋 英樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 奨励研究(A)

    Institution: 東北大学

    1988 - 1988

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Social Activities 8

  1. 文部科学省研究費補助金新学術領域研究審査委員

    2011/12/01 - 2012/01/31

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    文部科学省研究費補助金新学術領域研究（非コードRNA）の審査を行う

  2. 日本学術振興会科学研究費補助金（第1次）審査会専門委員会

    2011/12/01 - 2012/01/31

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    平成24年度日本学術振興会科学研究費補助金の第１次審査

  3. 日本学術振興会科学研究費補助金（第1次）審査会専門委員会

    2010/12 - 2011/01

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    平成23年度科学研究費補助金の第1次審査

  4. 日本学術振興会外国人特別研究員（第1次）審査会専門委員会

    2005/10 - 2006/07/31

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    平成18年度外国人特別研究員の第1次審査

  5. 日本学術振興会外国人特別研究員（欧米短期）審査会専門委員会

    2005/01 - 2006/01

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    外国人特別研究員（欧米短期）（平成17:第1-6回、平成18:第1回）の審査

  6. 平成16年度 第48回農業実験実習講習会（東北地区）

    2004/08/02 - 2004/08/06

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    農業高校の教員を対象に生物農薬についての講義と実習を行う。

  7. 第1回食と農コミュニケーションづくりの集い

    2006/12/02 -

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    農村部で働く方々を対象に、大学での教育・研究活動を紹介。

  8. 日本学術振興会産学協研究委員会 植物分子デザイン第178委員会

    2005/12/01 -

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    新たな産学協力研究の推進

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  1. 持続可能な農業生産のための新たな植物保護技術の開発

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    トマト青枯病抵抗性品種における青枯病抑制機構を、トマト品種自体の網羅的遺伝子発現解析により明らかにする。

  2. 有機栽培水稲に特徴的な微生物相と病害抑制効果

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    各地で栽培される有機栽培水稲や有機育苗培土に特徴的な微生物相を解析し、単離した微生物種によるイネ病害抑制効果を明らかにすることにより、安定的な有機栽培に関わる微生物的指標の策定を目指す。

  3. 低投入型農業のための生物農薬等新資材及びその利用技術の開発(微生物資材の誘導抵抗性の活性評価)

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    新規微生物資材の植物への抵抗性誘導による防除効果を科学的に証明するため、各微生物資材候補の選抜株の処理による植物の誘導抵抗活性の程度を評価する。

  4. 稲細胞間隙の微生物叢を指標とする有機水稲の病害抑制機能の解明

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    他参画課題の有機水稲圃の水稲および対象慣行水稲における細胞間隙の微生物叢を、分子生物学的手法により解析する。さらに、同水稲圃より採取した土壌を用いて育成した水稲のいもち病抵抗性と細胞間隙微生物叢を解析し、植物体内微生物叢と有機栽培水稲の病害抵抗性の関係を明らかにする。

  5. 生物防除微生物資材がもつ病害抵抗性誘導活性を迅速かつ高感度に評価できる

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    化学農薬は、生物毒性による環境への影響や薬剤耐性菌の出現などが問題となっており、現在、従来の農薬に代わる病害防除手段として、自然界に生息する非病原性微生物の利用が注目されている。植物は、病原体感染に対する防御機構を潜在的に備えているが、この非病原性微生物の中には、植物体に作用してその潜在的防御機構を活性化させることにより(=誘導抵抗性)、病害抑制効果を発揮するものが存在することが明らかになっている。これまでの研究により、すでにcDNAアレイ解析により誘導抵抗性発現に伴って発現が上昇する遺伝子（マーカーとなる遺伝子）を単離・同定している。本研究では、そのマーカー遺伝子の転写制御配列(プロモーター)を、蛍光タンパク質をコードするレポーターDNAと連結し、植物に形質転換することにより、誘導抵抗性活性を簡易的に評価できるレポーター植物を作出する。このレポーター植物を用いることにより、新規な抵抗性誘導型非病原微生物を、環境中からスクリーニングすることができる。化学農薬は、生物毒性を有し、人畜、水産物および環境に悪影響を及ぼすため、従来型の農薬に替わる、防除資材の開発が望まれている。国内外の農薬業界では、生物毒性をもたず抵抗性を誘導する次世代型農薬(Plant activator)の開発が進んでいる。本研究成果により、本レポーター植物を用いるスクリーニング法を確立すれば、従来型の農薬の使用を減らし、より安全性の高い微生物資材に代替されることになり、「環境保全に大きく寄与する」と考えられる。

  6. 病害抵抗性誘導化合物の活性を評価できるレポーター植物の開発

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    病害抵抗性誘導化合物の活性を高感度で迅速に検出できるレポーター植物を開発し、抵抗誘導活性評価系を構築する。

  7. エチレンシグナル系病害抵抗性誘導化合物の活性を検出できるレポーター植物の開発

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    化学農薬は、生物毒性による環境への影響や薬剤耐性菌の出現などが問題となっており、現在、従来の農薬に代わる次世代型農薬(Plant activator)の開発が進んでいる。植物は、病原体感染に対する防御機構を潜在的に備えているが、このPlant activatorは、殺菌活性をもたず、植物体に作用してその潜在的防御機構を活性化させることにより(=誘導抵抗性)、病害抑制効果を発揮する化合物である。本課題では、新規なPlant activatorのスクリーニングを迅速かつ効率的に行なうことができる検定植物の開発と、その実用化に向けた研究を行なう。申請者が単離した誘導抵抗性マーカー遺伝子(CAS)の転写制御配列に蛍光タンパク質コード領域を連結したDNAを形質転換した検定植物を作成し、緑色蛍光によりPlant activator活性を可視化するシステムを構築する。候補化合物を検定植物に茎葉散布することにより、発光を指標として、従来の生物検定法よりも迅速かつ効率的にPlant activator活性化合物の効力検定および選抜が可能となる。さらに、実際に新規Plant activatorの開発現場において本検定植物を試用し、その実用性を確認する。

  8. Pythium oligandrum (PO)施用作物における分子応答機構の解明

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    PO菌施用により植物に誘導される耐病性の分子機構解明

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