TOHOKU UNIVERSITY Researchers

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Kentaro Kato

Section

Graduate School of Agricultural Science

Job title

Professor

Degree

博士（獣医学）（東京大学）

researchmap

https://researchmap.jp/read0204467

J-GLOBAL ID

200901058306710377

e-Rad No.

30401178

Research History 7

2024/02 - Present

Tohoku University　Graduate School of Agricultural Science Filed Science Center
2019/04 - Present

Tohoku University　Graduate School of Agricultural Science
2017/04 - 2019/03

Obihiro University of Agriculture and Veterinary Medicine　National Research Center for Protozoan Diseases
2013/03 - 2017/03

Obihiro University of Agriculture and Veterinary Medicine　National Research Center for Protozoan Diseases
2007/04 - 2013/03

The University of Tokyo　Graduate School of Agricultural and Life Sciences
2005/01 - 2007/03

The University of Tokyo　Graduate School of Agricultural and Life Sciences
2003/04 - 2004/12

米国国立衛生研究所（NIH）　客員研究員

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Education 3

The University of Tokyo　Graduate School of Agricultural and Life Sciences

2000/04 - 2003/03
The University of Tokyo

1996/04 - 2000/03
The University of Tokyo　College of Arts and Sciences

1994/04 - 1996/03

Professional Memberships 5

JAPANESE SOCIETY OF TROPICAL MEDICINE
日本獣医寄生虫学会
日本寄生虫学会
日本ウイルス学会
日本獣医学会

Research Interests 3

ウイルス
原虫
感染症

Research Areas 4

Life sciences / Biomaterials /
Life sciences / Biomedical engineering /
Life sciences / Parasitology /
Life sciences / Veterinary medicine /

Awards 16

第6回竹内勤記念国際賞

2024/03　大山健康財団
第32回武見奨励賞

2022/12　武見記念生存科学研究基金
第7回伊藤記念財団賞

2022/06　伊藤記念財団
第8回三島海雲学術賞

2019/07　三島海雲記念財団
平成30年度北海道科学技術奨励賞

2019/02　北海道
平成29年度森永賞

2018/06　森永奉仕会
2017-2018年度日本獣医学会賞

2017/09　日本獣医学会
平成26年度若手農林水産研究者表彰

2014/11　農林水産技術会議
平成25年度森永奉仕会賞

2014/06　森永奉仕会
第11回日本農学進歩賞

2012/11　農学会
平成23年度科学技術分野文部科学大臣表彰若手科学者賞

2011/04　文部科学省
奨励賞

2010/05　第19回日本寄生虫学会
第1回日本獣医寄生虫学奨励賞

2010/03　日本獣医寄生虫学会
大会長賞

2006/03　第141回日本獣医学会
大会長賞

2002/09　第134回日本獣医学会
The Young Scientist Scholarship

2000/08　The XXIth World’s Poultry Congress

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Papers 154

Molecular characterization and zoonotic risk assessment of Cryptosporidium spp. in Philippine bats Peer-reviewed

Lin Xu, Yasuhiro Fukuda, Fumi Murakoshi, Phillip Alviola, Joseph Masangkay, Frances Cagayat Recuenco, Ayman Shehata, Tsutomu Omatsu, Hironori Bando, Hikaru Fujii, Yumi Une, Kentaro Kato

Food and Waterborne Parasitology　e00249-e00249　2024/12
Publisher: Elsevier BV
DOI： 10.1016/j.fawpar.2024.e00249 　

ISSN： 2405-6766
Efficacy of medicinal plants and their derived biomolecules against Plasmodium falciparum. International-journal Peer-reviewed

Umme Qulsum, Md Thoufic Anam Azad, Kentaro Kato

Parasitology international　103　102946-102946　2024/08/14

DOI： 10.1016/j.parint.2024.102946 　

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Many apicomplexan pathogens pose significant threats to humans and domestic animals, with the lack of effective drugs and drug resistance representing major challenges in disease management. To address this, the search for new and potent antimalarial drugs is crucial. Plant-based formulations offer a promising alternative for such drug development. Here, we evaluated the in vitro antiplasmodial activity of nine plant extracts, traditionally used to treat fever-like symptoms in Bangladesh. We assessed the antimalarial activity of plant extracts by using the Plasmodium falciparum 3D7 growth inhibition assay, an invasion assay, and a cytotoxicity assay. Of the nine plants studied, ethanolic and methanolic leaf extracts of Ficus hispida, Streblus asper, and Boerhavia repens exhibited high antiplasmodial activity, with IC50 values of 9.31, 4.13, 9.63 μg/ml (ethanolic) and 15.12, 6.63, 7.58 μg/ml (methanolic), respectively, and minimal toxicity (cell viability >80%). Clerodendrum viscosum displayed antiplasmodial effects with IC50 values of 28.90 μg/ml (ethanolic) and 30.57 μg/ml (methanolic). Adhatoda vasica, Mussaenda corymbosa, and Amaranthus spinosus ethanolic extracts showed antimalarial effects with IC50 values of 61.78 μg/ml, 66.31 μg/ml, and 64.14 μg/ml, respectively. However, methanolic extracts of A. vasica and A. spinosus had IC50 values >100 μg/ml. The ethanolic and methanolic extracts of A. vasica, A. spinosus, F. hispida, S. asper, and B. repens significantly reduced parasitemia by inhibiting invasion into erythrocytes. This study highlights the robust antimalarial activity and low cytotoxicity of leaf extracts of F. hispida, S. asper, and B. repens, indicating the presence of antimalarial compounds that warrant further investigation.
Comprehensive molecular epidemiology of Cryptosporidium species in Japan. International-journal Peer-reviewed

Mohammad Hazzaz Bin Kabir, Kentaro Kato

Parasitology international　102909-102909　2024/05/27

DOI： 10.1016/j.parint.2024.102909 　

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Cryptosporidium species, causing diarrheal illnesses in humans and animals worldwide, are under investigation for their molecular epidemiology in Japan. The study focuses on detecting Cryptosporidium species in humans, animals, water, and the environment, revealing three species in people: C. parvum, C. meleagridis, and C. hominis. Subtype IIa of the C. parvum gp60 gene is prevalent, indicating potential zoonotic transmission. Animal studies identified sixteen species, mainly cattle and pets, with C. parvum (subtype IIa) common in cattle and C. canis and C. felis prevalent in pets. Additionally, C. bovis and C. ryanae were found in cattle and sika deer. Knowledge gaps exist, particularly in water and environmental source typing, with limited research revealing five species and five genotypes, suggesting a significant role of water in transmission. Further research is needed to understand the molecular diversity and transmission dynamics across humans, animals, water, and the environment in Japan.
Examining the molecular epidemiology of Giardia and Eimeria species in Japan: a comprehensive review. Peer-reviewed

Mohammad Hazzaz Bin Kabir, Kentaro Kato

The Journal of veterinary medical science　86　(5)　563-574　2024/04/01

DOI： 10.1292/jvms.23-0525 　

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Globally, animals and humans suffer from diarrheal illnesses due to protozoan parasites such as Giardia and Eimeria species. The molecular epidemiology of these parasites in Japan is summarized in this review. In humans, researchers found only one main species of Giardia, which is most referred to as G. lamblia, but it's also known by different names like G. duodenalis or G. intestinalis. However, within this species, six assemblages (A, B, C, D, E, and F) were found in animals, and assemblage B was frequently recorded in human and monkey populations, whereas assemblages A and E were predominant in calves. Assemblage A was found in sika deer and assemblages A, C, D, and F were predominant in dogs, cats, and ferret. Eimeria bovis, E. zuernii, and other species found in animals made up the group of species known as Eimeria spp., with E. bovis and E. zuernii being the most common in cattle. Our review highlighted a notable lack of data investigations regarding these two pathogens in water and environmental sources. Giardia cysts were found in the few studies that have been done on water sources, suggesting that water may play a significant role in the transmission of Giardia species. Our review suggests that further research is necessary to fully comprehend the molecular diversity and dynamics of transmission of Giardia spp. and Eimeria spp. in humans, animals, and environmental sources in Japan.
Goethite and Hematite Nanoparticles Show Promising Anti-Toxoplasma Properties. International-journal Peer-reviewed

Kosei Ishii, Eiji Akahoshi, Oluyomi Stephen Adeyemi, Hironori Bando, Yasuhiro Fukuda, Tomoyuki Ogawa, Kentaro Kato

Pharmaceutics　16　(3)　2024/03/18

DOI： 10.3390/pharmaceutics16030413 　

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Toxoplasma gondii is an intracellular parasitic protozoan with a high infection rate in mammals, including humans, and birds. There is no effective vaccine, and treatment relies on antiparasitic drugs. However, existing antiprotozoal drugs have strong side effects and other problems; therefore, new treatment approaches are needed. Metal nanoparticles have attracted increased interest in the biomedical community in recent years because of their extremely high surface area to volume ratio and their unique reactivity that could be exploited for medicinal purposes. Previously, we confirmed the anti-Toxoplasma effects of gold, silver, and platinum nanoparticles, in a growth inhibition test. Here, we asked whether the anti-Toxoplasma effect could be confirmed with less expensive metal nanoparticles, specifically iron oxide nanoparticles (goethite and hematite). To improve the selective action of the nanoparticles, we modified the surface with l-tryptophan as our previous findings showed that the bio-modification of nanoparticles enhances their selectivity against T. gondii. Fourier-Transform Infrared Spectroscopy (FTIR) analysis confirmed the successful coating of the iron oxide nanoparticles with l-tryptophan. Subsequently, cytotoxicity and growth inhibition assays were performed. L-tryptophan-modified nanoparticles showed superior anti-Toxoplasma action compared to their naked nanoparticle counterparts. L-tryptophan enhanced the selective toxicity of the iron oxide nanoparticles toward T. gondii. The bio-modified nanoparticles did not exhibit detectable host cell toxicity in the effective anti-Toxoplasma doses. To elucidate whether reactive oxygen species contribute to the anti-Toxoplasma action of the bio-modified nanoparticles, we added Trolox antioxidant to the assay medium and found that Trolox appreciably reduced the nanoparticle-induced growth inhibition.
Detection of Developmental Asexual Stage-Specific RNA Editing Events in Plasmodium falciparum 3D7 Malaria Parasite. International-journal Peer-reviewed

Md Thoufic Anam Azad, Tatsuki Sugi, Umme Qulsum, Kentaro Kato

Microorganisms　12　(1)　2024/01/10

DOI： 10.3390/microorganisms12010137 　

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Transcriptional variation has been studied but post-transcriptional modification due to RNA editing has not been investigated in Plasmodium. We investigated developmental stage-specific RNA editing in selected genes in Plasmodium falciparum 3D7. We detected extensive amination- and deamination-type RNA editing at 8, 16, 24, 32, 40, and 46 h in tightly synchronized Plasmodium. Most of the editing events were observed in 8 and 16 h ring-stage parasites. Extensive A-to-G deamination-type editing was detected more during the 16 h ring stage (25%) than the 8 h ring stage (20%). Extensive U-to-C amination-type editing was detected more during the 16 h ring stage (31%) than the 8 h ring stage (22%). In 28S, rRNA editing converted the loop structure to the stem structure. The hemoglobin binding activity of PF3D7_0216900 was also altered due to RNA editing. Among the expressed 28S rRNA genes, PF3D7_0532000 and PF3D7_0726000 expression was higher. Increased amounts of the transcripts of these two genes were found, particularly PF3D7_0726000 in the ring stage and PF3D7_0532000 in the trophozoite and schizont stages. Adenosine deaminase (ADA) expression did not correlate with the editing level. This first experimental report of RNA editing will help to identify the editing machinery that might be useful for antimalarial drug discovery and malaria control.
Toxoplasma gondii chitinase-like protein TgCLP1 regulates the parasite cyst burden. International-journal Peer-reviewed

Hironori Bando, Yuho Murata, Yongmei Han, Tatsuki Sugi, Yasuhiro Fukuda, David J Bzik, Barbara A Fox, Kentaro Kato

Frontiers in cellular and infection microbiology　14　1359888-1359888　2024

DOI： 10.3389/fcimb.2024.1359888 　

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Toxoplasma, an important intracellular parasite of humans and animals, causes life-threatening toxoplasmosis in immunocompromised individuals. Although Toxoplasma secretory proteins during acute infection (tachyzoite, which divides rapidly and causes inflammation) have been extensively characterized, those involved in chronic infection (bradyzoite, which divides slowly and is surrounded by a cyst wall) remain uncertain. Regulation of the cyst wall is essential to the parasite life cycle, and polysaccharides, such as chitin, in the cyst wall are necessary to sustain latent infection. Toxoplasma secretory proteins during the bradyzoite stage may have important roles in regulating the cyst wall via polysaccharides. Here, we focused on characterizing the hypothetical T. gondii chitinase, chitinase-like protein 1 (TgCLP1). We found that the chitinase-like domain containing TgCLP1 is partially present in the bradyzoite microneme and confirmed, albeit partially, its previous identification in the tachyzoite microneme. Furthermore, although parasites lacking TgCLP1 could convert from tachyzoites to bradyzoites and make an intact cyst wall, they failed to convert from bradyzoites to tachyzoites, indicating that TgCLP1 is necessary for bradyzoite reactivation. Taken together, our findings deepen our understanding of the molecular basis of recrudescence and could contribute to the development of novel strategies for the control of toxoplasmosis.
In Vivo Efficacy of Curcumin and Curcumin Nanoparticle in Trypanosoma congolense, Broden 1904 (Kinetoplastea: Trypanosomatidae)-Infected Mice. International-journal Peer-reviewed

Nthatisi Innocentia Molefe-Nyembe, Oluyomi Stephen Adeyemi, Daisuke Kondoh, Kentaro Kato, Noboru Inoue, Keisuke Suganuma

Pathogens (Basel, Switzerland)　12　(10)　2023/10/09

DOI： 10.3390/pathogens12101227 　

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Curcumin (CUR) is known for its wide folkloric effects on various infections; however, its solubility status has remained a hindrance to its bioavailability in the host. This study evaluated the comparative effects of CUR and CUR-nanoparticle in vitro on T. congolense, T. b. brucei, and T. evansi. Additionally, CUR and CUR-nanoparticle anti-Trypanosoma efficacy were assessed in vivo against T. congolense. All the CUR-nanoparticles were two folds more effective on the T. congolense as compared to CUR in vitro, with recorded efficacy of 3.67 ± 0.31; 7.61 ± 1.22; and 6.40 ± 3.07 μM, while the CUR-nanoparticles efficacy was 1.56 ± 0.50; 28.16 ± 9.43 and 13.12 ± 0.13 μM on T. congolense, T. b. brucei, and T. evansi, respectively. Both CUR and CUR-nanoparticles displayed moderate efficacy orally. The efficacy of CUR and CUR-nanoparticles in vivo was influenced by solubility, presence of food, and treatment period. CUR-treated mice were not cured of the infection; however, the survival rate of the orally treated mice was significantly prolonged as compared with intraperitoneal-treated mice. CUR-nanoparticles resulted in significant suppression of parasitemia even though relapsed was observed. In conclusion, CUR and CUR-nanoparticles possess moderate efficacy orally on the trypanosomes as compared to the intraperitoneal treatment.
Antiplasmodial and interferon-gamma-modulating activities of the aqueous extract of stone breaker (Phyllanthus niruri Linn.) in malaria infection. International-journal Peer-reviewed

Temitope Olawale Jeje, Hironori Bando, Md Thoufic Anam Azad, Yasuhiro Fukuda, Ibukun Emmanuel Oluwafemi, Kentaro Kato

Parasitology international　97　102789-102789　2023/07/18

DOI： 10.1016/j.parint.2023.102789 　

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Plasmodium falciparum parasites are the primary cause of malaria across Africa. The problem of drug resistance to malaria is ever growing and novel therapeutic strategies need to be developed, particularly those targeting the parasite and also the host or host-pathogen interaction. Previous studies have shown that the development of cerebral malaria (CM) is related to dysregulation of the immune system in a murine malaria model of experimental cerebral malaria. It involves a complex interaction of events and interferon-gamma seems to be the unifying factor. Therefore, the antiplasmodial activity targeting the parasite and immunomodulatory strategies that reduce overall host inflammation, with IFN-γ in focus, could delay CM onset and prove beneficial in malaria infection therapy. Phyllanthus niruri is used to treat fever and other symptoms of malaria in Nigeria. Its modes of action as an anti-malarial remedy have not been exhaustively investigated. This study therefore examined the aqueous extract of P. niruri (PE) for its antiplasmodial activity in vitro using the Plasmodium falciparum HB3 strain. Furthermore, in vivo murine malaria model using the Plasmodium berghei ANKA strain was used to investigate its anti-malarial effects. We showed that PE has multiple anti-malarial effects, including anti-parasitic and host immunomodulatory activities. Co-culture of P. falciparum with PE and some of its phytoconstituents drastically reduced parasite number. PE also decreased parasitemia, and increased the survival of infected mice. We also observed that the integrity of the blood-brain barrier was maintained in the PE-treated mice. The results confirmed that PE showed moderate antiplasmodial activity. In vivo murine malaria model using P. berghei ANKA for experimental cerebral malaria revealed that PE suppressed parasite growth, and modulate the production of interferon-gamma. The findings demonstrate that PE affects malaria progression, targeting parasites and host cells.
L-tryptophan-titanium oxide nanoparticles showed selective anti-Toxoplasma gondii activity and improved host biocompatibility. International-journal Peer-reviewed

Oluyomi Stephen Adeyemi, Kosei Ishii, Kentaro Kato

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie　162　114597-114597　2023/03/27

DOI： 10.1016/j.biopha.2023.114597 　

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Toxoplasma gondii, the etiological agent of toxoplasmosis, currently affects nearly one-third of the human population. Treatment options for toxoplasmosis are limited, which underscores the need for new drugs. In the present study, we screened nanoparticles (NPs) of titanium dioxide (TiO2) and molybdenum (Mo) for their potential to inhibit the growth of T. gondii in vitro. NPs of TiO2 and Mo showed non-dose-dependent anti-T. gondii activity with EC50 values of 157.6 and 253 µg/mL, respectively. Previously, we showed that amino acid modification of NPs enhances their selective anti-parasite toxicity. Therefore, to enhance the selective anti-parasitic action of TiO2, we modified the NP surface using alanine, aspartate, arginine, cysteine, glutamate, tryptophan, tyrosine, and bovine serum albumin. The bio-modified TiO2 showed anti-parasite activity with EC50 values ranging from 45.7 to 286.4 µg/mL. At effective anti-parasite concentrations, modified-TiO2 showed no appreciable host cytotoxicity. Of the eight bio-modified TiO2, tryptophan-TiO2 showed the most promising anti-T. gondii specificity and improved host biocompatibility with a selectivity index (SI) of 49.1 versus 7.5 for TiO2 (note, pyrimethamine, a standard drug for toxoplasmosis, has an SI of 2.3). Furthermore, our data indicate that redox modulation may be part of the anti-parasite action of these NPs. Indeed, augmentation with trolox and l-tryptophan reversed the growth restriction caused by the tryptophan-TiO2 NPs. Collectively, these findings suggest that the parasite toxicity was selective and not a result of general cytotoxic action. Furthermore, surface modification with amino acids such as l-tryptophan not only enhanced the anti-parasitic action of TiO2 but also improved the host biocompatibility. Overall, our findings indicate that the nutritional requirements of T. gondii represent a viable target for the development of new and effective anti-T. gondii agents.
The In Vitro Anti-Parasitic Activities of Emodin toward Toxoplasma gondii. International-journal Peer-reviewed

Oluyomi Stephen Adeyemi, Kosei Ishii, Kentaro Kato

Pharmaceuticals (Basel, Switzerland)　16　(3)　2023/03/16

DOI： 10.3390/ph16030447 　

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Currently, toxoplasmosis affects nearly one-third of the world's population, but the available treatments have several limitations. This factor underscores the search for better therapy for toxoplasmosis. Therefore, in the current investigation, we investigated the potential of emodin as a new anti-Toxoplasma gondii while exploring its anti-parasitic mechanism of action. We explored the mechanisms of action of emodin in the presence and absence of an in vitro model of experimental toxoplasmosis. Emodin showed strong anti-T. gondii action with an EC50 value of 0.03 µg/mL; at this same effective anti-parasite concentration, emodin showed no appreciable host cytotoxicity. Likewise, emodin showed a promising anti-T. gondii specificity with a selectivity index (SI) of 276. Pyrimethamine, a standard drug for toxoplasmosis, had an SI of 2.3. The results collectively imply that parasite damage was selective rather than as a result of a broad cytotoxic effect. Furthermore, our data confirm that emodin-induced parasite growth suppression stems from parasite targets and not host targets, and indicate that the anti-parasite action of emodin precludes oxidative stress and ROS production. Emodin likely mediates parasite growth suppression through means other than oxidative stress, ROS production, or mitochondrial toxicity. Collectively, our findings support the potential of emodin as a promising and novel anti-parasitic agent that warrants further investigation.
The role of atypical MAP kinase 4 in the host interaction with Cryptosporidium parvum. International-journal Peer-reviewed

Nina Watanabe, Hironori Bando, Fumi Murakoshi, Riku Sakurai, Mohammad Hazzaz Bin Kabir, Yasuhiro Fukuda, Kentaro Kato

Scientific reports　13　(1)　1096-1096　2023/01/19

DOI： 10.1038/s41598-023-28269-w 　

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Cryptosporidium parvum is an apicomplexan parasite that causes severe zoonotic diarrhea in humans and calves. Since there are no effective treatments or vaccines for infants or immunocompromised patients, it is important to understand the molecular mechanisms of the parasite-host interaction for novel drug discovery. Mitogen-activated protein kinase (MAP kinase) is a key host factor in interactions between host and various pathogens, including parasites. Although the function of conventional MAP kinases against parasite infection has been investigated, that of atypical MAP kinases remains largely unknown. Therefore, we focused on one of the atypical MAP kinases, MAPK4, and its effect on C. parvum infection in human intestinal cells. Here, we report that MAPK4-deficient intestinal cells showed a significant reduction in C. parvum infection. We also show that host MAPK4 has a role in host cell survival from C. parvum infection. In addition, we show that C. parvum requires host MAPK4 for its successful invasion and asexual reproduction. Taken together, our data suggest that MAPK4 is an important host factor contributing to C. parvum infection in human intestinal cells.
Snf2 Proteins Are Required to Generate Gamete Pronuclei in Tetrahymena thermophila International-journal Peer-reviewed

Yasuhiro Fukuda, Takahiko Akematsu, Hironori Bando, Kentaro Kato

Microorganisms　10　(12)　2426-2426　2022/12/07
Publisher: MDPI AG
DOI： 10.3390/microorganisms10122426 　

eISSN： 2076-2607

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During sexual reproduction/conjugation of the ciliate Tetrahymena thermophila, the germinal micronucleus undergoes meiosis resulting in four haploid micronuclei (hMICs). All hMICs undergo post-meiotic DNA double-strand break (PM-DSB) formation, cleaving their genome. DNA lesions are subsequently repaired in only one ‘selected’ hMIC, which eventually produces gametic pronuclei. DNA repair in the selected hMIC involves chromatin remodeling by switching from the heterochromatic to the euchromatic state of its genome. Here, we demonstrate that, among the 15 Tetrahymena Snf2 family proteins, a core of the ATP-dependent chromatin remodeling complex in Tetrahymena, the germline nucleus specific Iswi in Tetrahymena IswiGTt and Rad5Tt is crucial for the generation of gametic pronuclei. In either gene knockout, the selected hMIC which shows euchromatin markers such as lysine-acetylated histone H3 does not appear, but all hMICs in which markers for DNA lesions persist are degraded, indicating that both IswiGTt and Rad5Tt have important roles in repairing PM-DSB DNA lesions and remodeling chromatin for the euchromatic state in the selected hMIC.
Identification of potent anti-Cryptosporidium new drug leads by screening traditional Chinese medicines. International-journal Peer-reviewed

Mohammad Hazzaz Bin Kabir, Frances Cagayat Recuenco, Nur Khatijah Mohd Zin, Nina Watanabe, Yasuhiro Fukuda, Hironori Bando, Kenichi Watanabe, Hiroki Bochimoto, Xuenan Xuan, Kentaro Kato

PLoS neglected tropical diseases　16　(11)　e0010947　2022/11

DOI： 10.1371/journal.pntd.0010947 　

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Cryptosporidium spp. are gastrointestinal opportunistic protozoan parasites that infect humans, domestic animals, and wild animals all over the world. Cryptosporidiosis is the second leading infectious diarrheal disease in infants less than 5 years old. Cryptosporidiosis is a common zoonotic disease associated with diarrhea in infants and immunocompromised individuals. Consequently, cryptosporidiosis is considered a serious economic, veterinary, and medical concern. The treatment options for cryptosporidiosis are limited. To address this problem, we screened a natural product library containing 87 compounds of Traditional Chinese Medicines for anti-Cryptosporidium compounds that could serve as novel drug leads and therapeutic targets against C. parvum. To examine the anti-Cryptosporidium activity and half-maximal inhibitory doses (EC50) of these compounds, we performed in vitro assays (Cryptosporidium growth inhibition assay and host cell viability assay) and in vivo experiments in mice. In these assays, the C. parvum HNJ-1 strain was used. Four of the 87 compounds (alisol-A, alisol-B, atropine sulfate, and bufotalin) showed strong anti-Cryptosporidium activity in vitro (EC50 values = 122.9±6.7, 79.58±13.8, 253.5±30.3, and 63.43±18.7 nM, respectively), and minimum host cell cytotoxicity (cell survival > 95%). Furthermore, atropine sulfate (200 mg/kg) and bufotalin (0.1 mg/kg) also showed in vivo inhibitory effects. Our findings demonstrate that atropine sulfate and bufotalin are effective against C. parvum infection both in vitro and in vivo. These compounds may, therefore, represent promising novel anti-Cryptosporidium drug leads for future medications against cryptosporidiosis.
Innate Immune Response and Inflammasome Activation During SARS-CoV-2 Infection. International-journal Peer-reviewed

Mohammad Islamuddin, Salman Ahmad Mustfa, Shehla Nasar Mir Najib Ullah, Usmaan Omer, Kentaro Kato, Shama Parveen

Inflammation　45　(5)　1849-1863　2022/08/11

DOI： 10.1007/s10753-022-01651-y 　

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The novel coronavirus SARS-CoV-2, responsible for the COVID-19 outbreak, has become a pandemic threatening millions of lives worldwide. Recently, several vaccine candidates and drugs have shown promising effects in preventing or treating COVID-19, but due to the development of mutant strains through rapid viral evolution, urgent investigations are warranted in order to develop preventive measures and further improve current vaccine candidates. Positive-sense-single-stranded RNA viruses comprise many (re)emerging human pathogens that pose a public health problem. Our innate immune system and, in particular, the interferon response form an important first line of defense against these viruses. Flexibility in the genome aids the virus to develop multiple strategies to evade the innate immune response and efficiently promotes their replication and infective capacity. This review will focus on the innate immune response to SARS-CoV-2 infection and the virus' evasion of the innate immune system by escaping recognition or inhibiting the production of an antiviral state. Since interferons have been implicated in inflammatory diseases and immunopathology along with their protective role in infection, antagonizing the immune response may have an ambiguous effect on the clinical outcome of the viral disease. This pathology is characterized by intense, rapid stimulation of the innate immune response that triggers activation of the Nod-like receptor family, pyrin-domain-containing 3 (NLRP3) inflammasome pathway, and release of its products including the pro-inflammatory cytokines IL-6, IL-18, and IL-1β. This predictive view may aid in designing an immune intervention or preventive vaccine for COVID-19 in the near future.
Thymoquinone Induced Leishmanicidal Effect via Programmed Cell Death in Leishmania donovani. International-journal Peer-reviewed

Mohammad Islamuddin, Abuzer Ali, Obaid Afzal, Amena Ali, Intzar Ali, Abdulmalik Saleh Alfawaz Altamimi, Mubarak A Alamri, Kentaro Kato, Shama Parveen

ACS omega　7　(12)　10718-10728　2022/03/29

DOI： 10.1021/acsomega.2c00467 　

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Visceral leishmaniasis (VL) or kala-azar is a vector-borne dreaded protozoal infection that is caused by the parasite Leishmania donovani. With increases in the dramatic infection rates, present drug toxicity, resistance, and the absence of an approved vaccine, the development of new antileishmanial compounds from plant sources remains the keystone for the control of visceral leishmaniasis. In this study, we evaluated the leishmanicidal effect of thymoquinone against L. donovani with an in vitro and ex vivo model. Thymoquinone exhibited potent antipromastigote activity with IC50 and IC90 concentrations achieved at 6.33 ± 1.21 and 20.71 ± 2.15 μM, respectively, whereas the IC50 and IC90 concentrations were found to be 7.83 ± 1.65 and 27.25 ± 2.20 μM against the intramacrophagic form of amastigotes, respectively. Morphological changes in promastigotes and growth reversibility study following treatment confirmed the leishmanicidal effect of thymoquinone. Further, thymoquinone exhibited leishmanicidal activities against L. donovani promastigote through cytoplasmic shrinkage, membrane blebbing, chromatin condensation, cellular and nuclear shrinkage, and DNA fragmentation, as observed under scanning and transmission electron microscopy analyses. The antileishmanial activity was exerted via programmed cell death as proved by exposure of phosphatidylserine, DNA nicking by TUNEL assay, and loss of mitochondrial membrane potential. Thymoquinone at a concentration of 200 μM was devoid of any cytotoxic effects against mammalian macrophage cells. Thymoquinone showed strong leishmanicidal activity against L. donovani, which is mediated via an apoptosis mode of parasitic cell death, and accordingly, thymoquinone may be the source of a new lead molecule for the cure of VL.
Development of Highly Sensitive Sandwich ELISA for the Early-Phase Diagnosis of Chikungunya Virus Utilizing rE2-E1 Protein. International-journal Peer-reviewed

Mohammad Islamuddin, Abuzer Ali, Wajihul Hasan Khan, Amena Ali, Syed Kazim Hasan, Mohd Abdullah, Kentaro Kato, Malik Zainul Abdin, Shama Parveen

Infection and drug resistance　15　4065-4078　2022

DOI： 10.2147/IDR.S347545 　

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Introduction: Chikungunya is caused by an alpha virus transmitted to humans by an infected mosquito. Infection is generally considered to be self-limiting and non-critical. Chikungunya infection may be diagnosed by severe joint pain with fever, but it is difficult to diagnose because the symptoms of chikungunya are common to many pathogens, including dengue fever. Diagnosis mainly depends on viral culture, reverse transcriptase polymerase chain reaction (RT-PCR), and IgM ELISA. Early and accurate diagnosis of the virus can be achieved by the application of PCR methods, but the high cost and the need for a thermal cycler restrict the use of such methods. On the other hand, antibody-based IgM ELISA is considered to be inexpensive, but antibodies against chikungunya virus (CHIKV) only develop after 4 days of infection, so it has limited application in the earlier diagnosis of viral infection and the management of patients. Because of these challenges, a simple antigen-based sensitive, specific, and rapid detection method is required for the early and accurate clinical diagnosis of chikungunya. Methods: The amino acid sequence of CHIKV ectodomain E1 and E2 proteins was analyzed using bioinformatics tools to determine the antigenic residues, particularly the B-cell epitopes and their characteristics. Recombinant E2-E1 CHIKV antigen was used for the development of polyclonal antibodies in hamsters and IgG was purified. Serological tests of 96 CHIKV patients were conducted by antigen-capture ELISA using primary antibodies raised against rCHIKV E2-E1 in hamsters and human anti-CHIKV antibodies. Results: We observed high specificity and sensitivity, of 100% and 95.8%, respectively, and these values demonstrate the efficiency of the test as a clinical diagnostic tool. There was no cross-reactivity with samples taken from dengue patients. Discussion: Our simple and sensitive sandwich ELISA for the early-phase detection of CHIKV infection may be used to improve the diagnosis of chikungunya.
The anti-parasite action of imidazole derivatives likely involves oxidative stress but not HIF-1α signaling. International-journal Peer-reviewed

Oluyomi Stephen Adeyemi, Abiodun Omokehinde Eseola, Winfried Plass, Kentaro Kato, Chiagoziem A Otuechere, Oluwakemi Josephine Awakan, Olubunmi Atolani, David Adeiza Otohinoyi, Tobiloba Christiana Elebiyo, Ikponmwosa Owen Evbuomwan

Chemico-biological interactions　349　109676-109676　2021/11/01

DOI： 10.1016/j.cbi.2021.109676 　

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BACKGROUND: Therapeutic options for toxoplasmosis are limited. This fact underscores ongoing research efforts to identify and develop better therapy. Previously, we reported the anti-parasitic potential of a new series of derivatives of imidazole. OBJECTIVE: In the current investigation, we attempted the investigation of the possible action mechanism of few promising anti-parasite imidazole derivatives namely C1 (bis-imidazole), C2 (phenyl-substituted 1H-imidazole) and C3 (thiophene-imidazole) METHODS: We evaluated if oxidative stress, hypoxia as well as metabolic reprogramming of host l-tryptophan pathway form part of the parasite growth inhibition by imidazoles. Anti-parasite assay was performed for imidazoles at concentrations ranging from 0 to 10 μM, while pyrimethamine was used as reference drug to validate assay. RESULTS: Imidazole compounds restricted parasite growth dose-dependently. However, in the presence of an antioxidant (Trolox), l-tryptophan and/or CoCl2 (chemical inducer of hypoxia), the growth inhibitory efficacy of imidazoles was appreciably abolished. Further, imidazole treatment led to elevated level of reactive oxygen species, while reducing parasite mitochondrial membrane potential compared with control. In contrast, imidazole had no effect on host HIF-1α level suggesting its exclusion in the anti-parasite action. CONCLUSION: Taken together, imidazole-based compounds might restrict parasite growth by causing oxidative stress. The findings provide new insight on the likely biochemical mechanisms of imidazoles as prospective anti-parasite therapy. Data gives new perspective that not only underscores the anti-parasite prospects of imidazoles, but implicates the host l-tryptophan pathway as a feasible treatment option for T. gondii infections.
Aqueous extract of Phyllanthus niruri protects against severe malaria by blocking erythrocyte invasion and modulating the host immune response

Jeje Temitope Olawale, Hironori Bando, Yasuhiro Fukuda, Ibukun Emmanuel Oluwafemi, Kentaro Kato

2021/07/10
Publisher: Cold Spring Harbor Laboratory
DOI： 10.1101/2021.07.09.451735 　

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<title>ABSTRACT</title><italic>Plasmodium falciparum</italic> parasites are the major cause of malaria across Africa. Due to the appearance of multi-drug resistant parasites, new antimalarial drugs are needed. The medicinal plant <italic>Phyllanthus niruri</italic> is being used to treat fever and other symptoms of malaria in Nigeria; however, little is known about its antimalarial mechanisms. Here, we show that aqueous extract of <italic>P. niruri</italic> (PE) has multiple antimalarial effects, including anti-parasitic and host immunomodulatory activity. We found that co-culture of <italic>P. falciparum</italic> with PE drastically reduced parasite number, but PE did not inhibit parasite development or rupture; rather, it blocked erythrocytes invasion. In addition, we identified Astragalin as one of the antimalarial compounds which are contained in PE. Moreover, we found that PE suppresses the inflammatory activity and apoptosis of immune cells (T cells) and astrocytes and neurons in the central nervous system (CNS). Furthermore, we confirmed that oral administration of PE to mice suppressed parasite growth, excessive inflammation, CNS dysfunction, and the development of experimental cerebral malaria in an <italic>in vivo</italic> murine malaria model. Our findings demonstrate that PE has multiple effects on malaria progression, targeting both parasite and host cells.
New Series of Imidazoles Showed Promising Growth Inhibitory and Curative Potential Against Trypanosoma Infection. International-journal Peer-reviewed

Oluyomi Stephen Adeyemi, Nthatisi Innocentia Molefe-Nyembe, Abiodun Omokehinde Eseola, Winfried Plass, Oluwatosin Kudirat Shittu, Ibrahim Olatunji Yunusa, Olubunmi Atolani, Ikponmwosa Owen Evbuomwan, Oluwakemi J Awakan, Keisuke Suganuma, Kentaro Kato

The Yale journal of biology and medicine　94　(2)　199-207　2021/06

eISSN： 1551-4056

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The Trypanosoma spp. cause animal and human trypanosomiasis characterized with appreciable health and economic burden mostly in developing nations. There is currently no effective therapy for this parasitic disease, due to poor drug efficacy, drug resistance, and unwanted toxicity, etc. Therefore, new anti-Trypanosoma agents are urgently needed. This study explored new series of imidazoles for anti-Trypanosoma properties in vitro and in vivo. The imidazoles showed moderate to strong and specific action against growth of T. congolense. For example, the efficacy of the imidazole compounds to restrict Trypanosoma growth in vitro was ≥ 12-fold specific towards T. congolense relative to the mammalian cells. Additionally, the in vivo study revealed that the imidazoles exhibited promising anti-Trypanosoma efficacy corroborating the in vitro anti-parasite capacity. In particular, three imidazole compounds (C1, C6, and C8) not only cleared the systemic parasite burden but cured infected rats after no death was recorded. On the other hand, the remaining five imidazole compounds (C2, C3, C4, C5, and C7) drastically reduced the systemic parasite load while extending survival time of the infected rats by 14 days as compared with control. Untreated control died 3 days post-infection, while the rats treated with diminazene aceturate were cured comparable to the results obtained for C1, C6, and C8. In conclusion, this is the first study demonstrating the potential of these new series of imidazoles to clear the systemic parasite burden in infected rats. Furthermore, a high selectivity index of imidazoles towards T. congolense in vitro and the oral LD50 in rats support anti-parasite specific action. Together, findings support the anti-parasitic prospects of the new series of imidazole derivatives.
Inhibition of Chikungunya Virus Infection by 4-Hydroxy-1-Methyl-3-(3-morpholinopropanoyl)quinoline-2(1H)-one (QVIR) Targeting nsP2 and E2 Proteins. International-journal Peer-reviewed

Mohammad Islamuddin, Obaid Afzal, Wajihul Hasan Khan, Malik Hisamuddin, Abdulmalik Saleh Alfawaz Altamimi, Ibraheem Husain, Kentaro Kato, Mubarak A Alamri, Shama Parveen

ACS omega　6　(14)　9791-9803　2021/04/13

DOI： 10.1021/acsomega.1c00447 　

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The re-emergence of Chikungunya virus (CHIKV) infection in humans with no approved antiviral therapies or vaccines is one of the major problems with global significance. In the present investigation, we screened 80 in-house quinoline derivatives for their anti-CHIKV activity by computational techniques and found 4-hydroxy-1-methyl-3-(3-morpholinopropanoyl)quinoline-2(1H)-one (QVIR) to have potential binding affinities with CHIKV nsP2 and E2 glycoproteins. QVIR was evaluated in vitro for its anti-CHIKV potential. QVIR showed strong inhibition of CHIKV infection with an EC50 (50% effective concentration) value of 2.2 ± 0.49 μM without significant cytotoxicity (CC50 > 200 μM) and was chosen for further elucidation of its antiviral mechanism. The infectious viral particle formation was abolished by approximately 72% at a QVIR concentration of 20 μM during infection in the BHK-21 cell line, and the CHIKV RNA synthesis was diminished by 84% for nsP2 as well as 74% for E2, whereas the levels of viral proteins were decreased by 69.9% for nsP2 and 53.9% for E2. Flow cytometry analysis confirmed a huge decline in the expression of viral nsP2 and E2 proteins by 71.84 and 67.7%, respectively. Time of addition experiments indicated that QVIR inhibited viral infection at early and late stages of viral replication cycle, and the optimal inhibition was observed at 16 h post infection. The present study advocates for the first time that QVIR acts as a substantial and potent inhibitor against CHIKV and might be as an auspicious novel drug candidate for the development of therapeutic agents against CHIKV infections.
THE EFFICACY OF MARINE NATURAL PRODUCTS AGAINST PLASMODIUM FALCIPARUM. International-journal

Yukihiro Goto, Rie Kamihira, Yoichi Nakao, Motohiro Nonaka, Ryo Takano, Xuenan Xuan, Kentaro Kato

The Journal of parasitology　107　(2)　284-288　2021/03/01

DOI： 10.1645/20-93 　

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Malaria remains one of the most important infectious diseases in the world. In 2017 alone, approximately 219 million people were infected with malaria, and 435,000 people died of this disease. Plasmodium falciparum, which causes falciparum malaria, is becoming resistant to artemisinin (ART) in Southeast Asia; therefore, new antimalarial drugs are urgently needed. Some excellent antimalarial drugs, such as quinine and ART, were originally obtained from plants. Hence, we analyzed the antimalarial effects of marine natural products to find new antimalarial agents. We used a malaria growth inhibition assay to determine the antimalarial ability and half-maximal inhibitory concentration (IC50) values of the marine organism-derived compounds. Three compounds (kapakahine A, kapakahine B, and kulolide-1) showed antimalarial effects, and one (kapakahine F) showed selective antimalarial effects on the Dd2 clone. Although the IC50 values obtained for these compounds were greater than that of ART, their potency against P. falciparum is sufficient to warrant further investigation of these compounds as possible drug leads.
Depletion of Intracellular Glutamine Pools Triggers Toxoplasma gondii Stage Conversion in Human Glutamatergic Neurons. International-journal

Hironori Bando, Yasuhiro Fukuda, Nina Watanabe, Jeje Temitope Olawale, Kentaro Kato

Frontiers in cellular and infection microbiology　11　788303-788303　2021

DOI： 10.3389/fcimb.2021.788303 　

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Toxoplasma gondii chronically infects the brain as latent cysts containing bradyzoites and causes various effects in the host. Recently, the molecular mechanisms of cyst formation in the mouse brain have been elucidated, but those in the human brain remain largely unknown. Here, we show that abnormal glutamine metabolism caused by both interferon-γ (IFN-γ) stimulation and T. gondii infection induce cyst formation in human neuroblastoma cells regardless of the anti-T. gondii host factor nitric oxide (NO) level or Indoleamine 2,3-dioxygenase-1 (IDO1) expression. IFN-γ stimulation promoted intracellular glutamine degradation in human neuronal cells. Additionally, T. gondii infection inhibited the mRNA expression of the host glutamine transporters SLC38A1 and SLC38A2. These dual effects led to glutamine starvation and triggered T. gondii stage conversion in human neuronal cells. Furthermore, these mechanisms are conserved in human iPSC-derived glutamatergic neurons. Taken together, our data suggest that glutamine starvation in host cells is an important trigger of T. gondii stage conversion in human neurons.
Nullscript inhibits Cryptosporidium and Toxoplasma growth. International-journal Peer-reviewed

Fumi Murakoshi, Hironori Bando, Tatsuki Sugi, Oluyomi Stephen Adeyemi, Motohiro Nonaka, Takaaki Nakaya, Kentaro Kato

International journal for parasitology. Drugs and drug resistance　14　159-166　2020/12

DOI： 10.1016/j.ijpddr.2020.10.004 　

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Cryptosporidium and Toxoplasma are parasites that have caused problems worldwide. Cryptosporidium causes severe watery diarrhoea and may be fatal in immunocompromised patients and in infants. Nitazoxanide is the only agent currently approved by the FDA, but its efficacy is limited. Toxoplasmosis is also a problem in the immunocompromised, as currently available treatment options have limited efficacy and patient tolerance can be poor. In the present investigation, we screened libraries of epigenetic compounds to identify those that inhibited C. parvum growth. Nullscript was identified as a compound with an inhibitory effect on C. parvum and T. gondii growth, and was less toxic to host cells. Nullscript was also able to significantly decrease oocyst excretion in C. parvum-infected SCID mice.
Molecular detection of genotypes and subtypes of Cryptosporidium infection in diarrheic calves, lambs, and goat kids from Turkey. International-journal Peer-reviewed

Mohammad Hazzaz Bin Kabir, Onur Ceylan, Ceylan Ceylan, Ayman Ahmed Shehata, Hironori Bando, Mohamed Ibrahim Essa, Xuenan Xuan, Ferda Sevinc, Kentaro Kato

Parasitology international　79　102163-102163　2020/12
Publisher: Elsevier BV
DOI： 10.1016/j.parint.2020.102163 　

ISSN： 1383-5769

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The studies on Cryptosporidium infections of animals in Turkey mostly rely on microscopic observation. Few data are available regarding the prevalence of Cryptosporidium genotypes and subtypes infection. The aim of this study is to analyse the detection of Cryptosporidium genotypes and subtypes from young ruminants. A total of 415 diarrheic fecal specimens from young ruminants were examined for the Cryptosporidium detection by use of nested PCR of the small subunit ribosomal RNA (SSU rRNA) gene and the highly polymorphic 60 kDa glycoprotein (gp60) gene followed by sequence analyses. The results of this study revealed that 25.6% (106 of 415) of the specimens were positive for Cryptosporidium spp. infection. We identified 27.4% (91/333), 19.4% (13/67), and 13.4% (2/15) of positivity in calves, lambs and goat kids, respectively. Genotyping of the SSU rRNA indicated that almost all positive specimens were of C. parvum, except for one calf which was of C. bovis. Sequence analysis of the gp60 gene revealed the most common zoonotic subtypes (IIa and IId) of C. parvum. We detected 11 subtypes (IIaA11G2R1, IIaA11G3R1, IIaA12G3R1, IIaA13G2R1, IIaA13G4R1, IIaA14G1R1, IIaA14G3R1, IIaA15G2R1, IIdA16G1, IIdA18G1, IIdA22G1); three of them (IIaA12G3R1, IIaA11G3R1 and IIaA13G4R1) was novel subtypes found in calves and lambs. Additionally, three subtypes (IIaA11G2R1, IIaA14G3R1, and IIdA16G1) were detected in young ruminants for the first time in Turkey. These results indicate the high infection of Cryptosporidium in Turkey and propose that young ruminants are likely a major reservoir of C. parvum and a potential source of zoonotic transmission.
Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan. International-journal Peer-reviewed

Mohammad Hazzaz Bin Kabir, Megumi Itoh, Ayman Ahmed Shehata, Hironori Bando, Yasuhiro Fukuda, Fumi Murakoshi, Atsushi Fujikura, Hiroaki Okawa, Takuto Endo, Akira Goto, Masayuki Kachi, Toshie Nakayama, Yuto Kano, Shoko Oishi, Konosuke Otomaru, Mohamed Ibrahim Essa, Kei Kazama, Xuenan Xuan, Kentaro Kato

Parasitology international　78　102153-102153　2020/10

DOI： 10.1016/j.parint.2020.102153 　

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Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of hosts including humans, and domestic and wild animals. The aim of this study was to molecularly characterize the Cryptosporidium spp. found in calf faeces in Japan. A total of 80 pre-weaned beef and dairy calves' diarrhoeic faecal specimens were collected from nine different prefectures in Japan. A nested polymerase chain reaction targeting the small subunit 18S rRNA and GP60 genes were used to detect the Cryptosporidium genotypes and subtypes. 83.8% (67 out of 80) of the specimens were positive for Cryptosporidium spp.; Cryptosporidium was found in both beef and dairy calves. Cryptosporidium parvum was the predominant species, detected in 77.5% (31/40) of beef calves and 80% (32/40) of dairy calves. Cryptosporidium bovis was also detected, 5.0% (2/40) of dairy calves, and C. ryanae was also found 2.5% (1/40) of dairy calves. One mixed-species infection, 2.5% (1/40) was detected in a beef calf having C. parvum, and C. ryanae. We detected the most common subtype of C. parvum (i.e., IIaA15G2R1), as well as other subtypes (i.e., IIaA14G3R1, IIaA14G2R1, and IIaA13G1R1) that have not previously been detected in calves in Japan. Our results demonstrate the widespread diversity of Cryptosporidium infection in calves in Japan.
Imidazole derivatives as antiparasitic agents and use of molecular modeling to investigate the structure-activity relationship. International-journal Peer-reviewed

Oluyomi Stephen Adeyemi, Abiodun Omokehinde Eseola, Winfried Plass, Olubunmi Atolani, Tatsuki Sugi, Yongmei Han, Gaber El-Saber Batiha, Kentaro Kato, Oluwakemi Josephine Awakan, Tomilola Debby Olaolu, Charles Obiora Nwonuma, Omokolade Alejolowo, Akinyomade Owolabi, Damilare Rotimi, Omowumi Titilola Kayode

Parasitology research　119　(6)　1925-1941　2020/06

DOI： 10.1007/s00436-020-06668-6 　

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Toxoplasmosis is a common parasitic disease caused by Toxoplasma gondii. Limitations of available treatments motivate the search for better therapies for toxoplasmosis. In this study, we synthesized a series of new imidazole derivatives: bis-imidazoles (compounds 1-8), phenyl-substituted 1H-imidazoles (compounds 9-19), and thiopene-imidazoles (compounds 20-26). All these compounds were assessed for in vitro potential to restrict the growth of T. gondii. To explore the structure-activity relationships, molecular analyses and bioactivity prediction studies were performed using a standard molecular model. The in vitro results, in combination with the predictive model, revealed that the imidazole derivatives have excellent selectivity activity against T. gondii versus the host cells. Of the 26 compounds screened, five imidazole derivatives (compounds 10, 11, 18, 20, and 21) shared a specific structural moiety and exhibited significantly high selectivity (> 1176 to > 27,666) towards the parasite versus the host cells. These imidazole derivatives are potential candidates for further studies. We show evidence that supports the antiparasitic action of the imidazole derivatives. The findings are promising in that they reinforce the prospects of imidazole derivatives as alternative and effective antiparasitic therapy as well as providing evidence for a probable biological mechanism.
Prevalence and molecular characterization of Cryptosporidium species in poultry in Bangladesh. International-journal Peer-reviewed

Mohammad Hazzaz Bin Kabir, Yongmei Han, Seung-Hun Lee, Arifin Budiman Nugraha, Frances Recuenco, Fumi Murakoshi, Xuenan Xuan, Kentaro Kato

One health (Amsterdam, Netherlands)　9　100122-100122　2020/06

DOI： 10.1016/j.onehlt.2020.100122 　

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Cryptosporidium is an opportunistic parasite that has been reported in >30 avian hosts worldwide, however, there is no information regarding Cryptosporidium spp. in poultry in Bangladesh. Accordingly, we investigated the prevalence of Cryptosporidium spp. in poultry at open live bird markets in Bangladesh. A total of 197 samples were randomly collected from poultry at open live bird markets in Bangladesh and screened for the detection of Cryptosporidium. Initial microscopic examination revealed Cryptosporidium spp. was observed in 19.8% (39/197) of the poultry specimens. Subsequent nested PCR targeting the 18S rRNA gene revealed that 15.7% (31/197) of the samples were Cryptosporidium positive. Of these 31 samples, 17 were Cryptosporidium baileyi (8.7%), 12 were Cryptosporidium meleagridis (6.0%), and 2 were Cryptosporidium parvum (1.0%). Nucleotide sequence analysis of the GP60 gene of the C. meleagridis revealed that two subtypes (IIIbA21G1R1 and IIIbA23G1R1), which were found in broiler, native and sonali chickens and a pigeon, matched those previously reported in humans and poultry. We identified two novel subtypes (IIIbA21G2R1 and IIIbA20G2R1) in sonali chickens, a broiler chicken and a layer chicken. We also amplified the GP60 gene of C. parvum and found two subtypes (IIaA11G2R1 and IIaA13G2R1) in a sonali and a broiler chicken that were previously reported in calf. These findings suggest that poultry can be a source of cryptosporidial infections for humans and animals in Bangladesh. This is the first molecular investigation of Cryptosporidium genotypes and subtypes in poultry at open live bird markets in Bangladesh.
Screening of compound libraries for inhibitors of Toxoplasma growth and invasion. International-journal Peer-reviewed

Yongmei Han, Oluyomi Stephen Adeyemi, Mohammad Hazzaz Bin Kabir, Kentaro Kato

Parasitology research　119　(5)　1675-1681　2020/05

DOI： 10.1007/s00436-020-06673-9 　

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Toxoplasma gondii can infect virtually all warm-blooded animals, including humans. It can differentiate between rapidly replicating tachyzoites that cause acute infection and slowly growing bradyzoites in tissue cysts. Treatment options for toxoplasmosis are challenging because current therapies cannot eradicate the latent T. gondii infection that is mainly caused by the bradyzoite forms. Accordingly, recurrence of infection is a problem for immunocompromised patients and congenitally infected patients. Protein kinases have been widely studied in eukaryotic cells, and while little is known about signaling in Toxoplasma infection, it is likely that protein kinases play a key role in parasite proliferation, differentiation, and probably invasion. To identify optimized new kinase inhibitors for drug development against T. gondii, we screened a library of kinase inhibitor compounds for anti-Toxoplasma activity and host cell cytotoxicity. Pyrimethamine served as a positive control and 0.5% DMSO was used as a negative control. Among the 80 compounds screened, 6 compounds demonstrated ≥ 80% parasite growth inhibition at concentrations at which 5 compounds did not suppress host cell viability, while 3 kinase inhibitors (Bay 11-7082, Tyrphostin AG 1295 and PD-98059) had suppressive effects individually on parasite growth and host cell invasion, but did not strongly induce bradyzoite formation.
Comparative histological studies on properties of polysaccharides secreted by vomeronasal glands of eight Laurasiatheria species. International-journal Peer-reviewed

Daisuke Kondoh, Jumpei Tomiyasu, Raito Itakura, Mizuho Sugahara, Masashi Yanagawa, Kenichi Watanabe, Phillip A Alviola, Sheryl A Yap, Edison A Cosico, Florante A Cruz, Ariel R Larona, Allen J F Manalad, Joseph S Masangkay, Yuki Sugiura, Shigeru Kyuwa, Shumpei Watanabe, Yumi Une, Tsutomu Omatsu, Hironori Bando, Kentaro Kato

Acta histochemica　122　(3)　151515-151515　2020/04

DOI： 10.1016/j.acthis.2020.151515 　

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Most mammalian species have a vomeronasal organ that detects specific chemical substances, such as pheromones. Mucous fluid covering the vomeronasal sensory epithelium is secreted by vomeronasal glands, and the properties of these fluids have been suggested to be involved in chemical detection. Histological studies using periodic acid-Schiff (PAS) and Alcian blue pH 2.5 (AB) stains, which respectively detect natural and acidic polysaccharides, have suggested variations in the nature of the vomeronasal glands among species. Here, we investigated the responsivity of the vomeronasal glands to PAS and AB stains in eight Laurasiatheria species. All species studied herein possessed vomeronasal glands that stained positive for PAS, like other many reported species. The vomeronasal glands of dogs and minks - like rodents, were AB-negative, whereas those of cows, goats, sika deer, musk shrews and two bat species were positive. Considering the present findings and previous reports, the vomeronasal glands in most of Laurasiatheria species appear to be fundamentally abundant in acidic polysaccharides, whereas those in carnivores essentially contains neutral polysaccharides.
Screening of a library of traditional Chinese medicines to identify compounds and extracts which inhibit Toxoplasma gondii growth. Peer-reviewed

Harunobu Saito, Yuho Murata, Motohiro Nonaka, Kentaro Kato

The Journal of veterinary medical science　82　(2)　184-187　2020/02/18

DOI： 10.1292/jvms.19-0241 　

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Toxoplasma gondii can cause severe encephalitis in immunocompromised patients. Although pyrimethamine and sulphadiazine have been standard therapeutic agents for the treatment of acute toxoplasmosis, they have toxic side effects. Therefore, there is a need to identify new drugs that are less toxic. Some traditional Chinese medicines (TCMs) have shown good efficacy in controlling T. gondii replication in mouse models. Here, we screened a natural product library comprising TCMs with the aim of identifying compounds and extracts with anti-toxoplasmosis activities. We found several hit compounds and extracts that could be candidates for new drugs against T. gondii infection.
Development of a highly sensitive method for the detection of cryptosporidium parvum virus type 1 (Cspv1) Peer-reviewed

Ayman Ahmed Shehata, Hironori Bando, Yasuhiro Fukuda, Mohammad Hazzaz Bin Kabir, Fumi Murakoshi, Megumi Itoh, Atsushi Fujikura, Hiroaki Okawa, Takuto Endo, Akira Goto, Masayuki Kachi, Toshie Nakayama, Yuto Kano, Shoko Oishi, Konosuke Otomaru, Kei Kazama, Mohamed Ibrahim Essa, Kentaro Kato

Japanese Journal of Veterinary Research　68　(3)　159-170　2020

DOI： 10.14943/jjvr.68.3.159 　

ISSN： 0047-1917
Tree of motility - A proposed history of motility systems in the tree of life. International-journal Peer-reviewed

Makoto Miyata, Robert C Robinson, Taro Q P Uyeda, Yoshihiro Fukumori, Shun-Ichi Fukushima, Shin Haruta, Michio Homma, Kazuo Inaba, Masahiro Ito, Chikara Kaito, Kentaro Kato, Tsuyoshi Kenri, Yoshiaki Kinosita, Seiji Kojima, Tohru Minamino, Hiroyuki Mori, Shuichi Nakamura, Daisuke Nakane, Koji Nakayama, Masayoshi Nishiyama, Satoshi Shibata, Katsuya Shimabukuro, Masatada Tamakoshi, Azuma Taoka, Yosuke Tashiro, Isil Tulum, Hirofumi Wada, Ken-Ichi Wakabayashi

Genes to cells : devoted to molecular & cellular mechanisms　25　(1)　6-21　2020/01

DOI： 10.1111/gtc.12737 　

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Motility often plays a decisive role in the survival of species. Five systems of motility have been studied in depth: those propelled by bacterial flagella, eukaryotic actin polymerization and the eukaryotic motor proteins myosin, kinesin and dynein. However, many organisms exhibit surprisingly diverse motilities, and advances in genomics, molecular biology and imaging have showed that those motilities have inherently independent mechanisms. This makes defining the breadth of motility nontrivial, because novel motilities may be driven by unknown mechanisms. Here, we classify the known motilities based on the unique classes of movement-producing protein architectures. Based on this criterion, the current total of independent motility systems stands at 18 types. In this perspective, we discuss these modes of motility relative to the latest phylogenetic Tree of Life and propose a history of motility. During the ~4 billion years since the emergence of life, motility arose in Bacteria with flagella and pili, and in Archaea with archaella. Newer modes of motility became possible in Eukarya with changes to the cell envelope. Presence or absence of a peptidoglycan layer, the acquisition of robust membrane dynamics, the enlargement of cells and environmental opportunities likely provided the context for the (co)evolution of novel types of motility.
Three-dimensional fine structure of feeder organelle in Cryptosporidium parvum. International-journal Peer-reviewed

Hiroki Bochimoto, Daisuke Kondoh, Yo Ishihara, Mohammad Hazzaz Bin Kabir, Kentaro Kato

Parasitology international　73　101958-101958　2019/12

DOI： 10.1016/j.parint.2019.101958 　

ISSN： 1383-5769

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Feeder organelles of Cryptosporidium are the convoluted structures located at the host-parasite interface that uptake of nutrients from host cells. Although the ultrastructure of feeder organelles has been summarized as being highly invaginated structure, the three-dimensional form remains uncertain. Osmium-maceration scanning electron microscopy (OS-SEM) allows visualization of the three-dimensional ultrastructure after removing soluble proteins. Here, we assessed C. parvum attached to mouse ileal epithelial cells using transmission electron microscopy (TEM) and OS-SEM. Feeder organelles visualized by TEM as aggregated structures of concentrically-, vertically- and randomly-lined bars comprised a complex reticulated network of stacked flat bursiform, ring-shaped bursiform and reticulated tubular membranes on OS-SEM. These findings suggested that the feeder organelles are more complex than was previously thought.
Repurposing existing drugs: identification of irreversible IMPDH inhibitors by high-throughput screening. International-journal Peer-reviewed

Albertus Eka Yudistira Sarwono, Shinya Mitsuhashi, Mohammad Hazzaz Bin Kabir, Kengo Shigetomi, Tadashi Okada, Fumina Ohsaka, Satoko Otsuguro, Katsumi Maenaka, Makoto Igarashi, Kentaro Kato, Makoto Ubukata

Journal of enzyme inhibition and medicinal chemistry　34　(1)　171-178　2019/12

DOI： 10.1080/14756366.2018.1540474 　

ISSN： 1475-6366

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Inosine 5'-monophosphate dehydrogenase (IMPDH) is an essential enzyme for the production of guanine nucleotides. Disruption of IMPDH activity has been explored as a therapeutic strategy for numerous purposes, such as for anticancer, immunosuppression, antiviral, and antimicrobial therapy. In the present study, we established a luciferase-based high-throughput screening system to identify IMPDH inhibitors from our chemical library of known bioactive small molecules. The screening of 1400 compounds resulted in the discovery of three irreversible inhibitors: disulfiram, bronopol, and ebselen. Each compound has a distinct chemical moiety that differs from other reported IMPDH inhibitors. Further evaluation revealed that these compounds are potent inhibitors of IMPDHs with kon values of 0.7 × 104 to 9.3 × 104 M-1·s-1. Both disulfiram and bronopol exerted similar degree of inhibition to protozoan and mammalian IMPDHs. Ebselen showed an intriguing difference in mode of inhibition for different IMPDHs, with reversible and irreversible inhibition to each Cryptosporidium parvum IMPDH and human IMPDH type II, respectively. In the preliminary efficacy experiment against cryptosporidiosis in severe combined immunodeficiency (SCID) mouse, a decrease in the number of oocyst shed was observed upon the oral administration of disulfiram and bronopol, providing an early clinical proof-of-concept for further utilization of these compounds as IMPDH inhibitors.
A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes. International-journal Peer-reviewed

Ryo Takano, Hiroko Kozuka-Hata, Daisuke Kondoh, Hiroki Bochimoto, Masaaki Oyama, Kentaro Kato

iScience　19　703-714　2019/09/27

DOI： 10.1016/j.isci.2019.07.035 　

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The pathogenesis of malaria parasites depends on host erythrocyte modifications that are facilitated by parasite proteins exported to the host cytoplasm. These exported proteins form a trafficking complex in the host cytoplasm that transports virulence determinants to the erythrocyte surface; this complex is thus essential for malaria virulence. Here, we report a comprehensive interaction network map of this complex. We developed authentic, unbiased, highly sensitive proteomic approaches to determine the proteins that interact with a core component of the complex, SBP1 (skeleton-binding protein 1). SBP1 interactomes revealed numerous exported proteins and potential interactors associated with SBP1 intracellular trafficking. We identified several host-parasite protein interactions and linked the exported protein MAL8P1.4 to Plasmodium falciparum virulence in infected erythrocytes. Our study highlights the complicated interplay between parasite and host proteins in the host cytoplasm and provides an interaction dataset connecting dozens of exported proteins required for P. falciparum virulence.
Nanoparticles show potential to retard bradyzoites in vitro formation of Toxoplasma gondii Peer-reviewed

Oluyomi Stephen Adeyemi, Yuho Murata, Tatsuki Sugi, Yongmei Han, Kentaro Kato

FOLIA PARASITOLOGICA　66　2019/02

DOI： 10.14411/fp.2019.001 　

ISSN： 0015-5683

eISSN： 1803-6465
How does Toxoplama gondii invade host cells? Peer-reviewed

Kato K

The Journal of veterinary medical science　80　(11)　1702-1706　2018/10

DOI： 10.1292/jvms.18-0344 　

ISSN： 0916-7250
Molecular and histopathological characterization of Cryptosporidium and Eimeria species in bats in Japan. Peer-reviewed

Murakoshi F, Koyama K, Akasaka T, Horiuchi N, Kato K

The Journal of veterinary medical science　80　(9)　1395-1399　2018/09

DOI： 10.1292/jvms.18-0130 　

ISSN： 0916-7250

eISSN： 1347-7439
Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts. Peer-reviewed

Nonaka M, Murata Y, Takano R, Han Y, Kabir MHB, Kato K

Malaria journal　17　(1)　244　2018/06/25

DOI： 10.1186/s12936-018-2392-4 　

ISSN： 1475-2875
Exploring amino acid-capped nanoparticles for selective anti-parasitic action and improved host biocompatibility Peer-reviewed

Oluyomi Stephen Adeyemi, Yuho Murata, Tatsuki Sugi, Yongmei Han, Kentaro Kato

Journal of Biomedical Nanotechnology　14　(5)　847-867　2018/05/01

DOI： 10.1166/jbn.2018.2544 　

ISSN： 1550-7041 1550-7033
Toxoplasma gondii RON4 binds to heparan sulfate on the host cell surface Peer-reviewed

Hitoshi Takemae, Kyousuke Kobayashi, Tatsuki Sugi, Yongmei Han, Haiyan Gong, Akiko Ishiwa, Frances C. Recuenco, Fumi Murakoshi, Ryo Takano, Yuho Murata, Kisaburo Nagamune, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato

Parasitology International　67　(2)　123-130　2018/04/01

DOI： 10.1016/j.parint.2017.10.008 　

ISSN： 1873-0329 1383-5769
Screening of chemical compound libraries identified new anti-Toxoplasma gondii agents Peer-reviewed

Oluyomi Stephen Adeyemi, Tatsuki Sugi, Yongmei Han, Kentaro Kato

Parasitology Research　117　(2)　355-363　2018/02/01

DOI： 10.1007/s00436-017-5698-1 　

ISSN： 1432-1955 0932-0113
Metal nanoparticles restrict the growth of protozoan parasites. International-journal Peer-reviewed

Oluyomi Stephen Adeyemi, Nthatisi Innocentia Molefe, Oluwakemi Josephine Awakan, Charles Obiora Nwonuma, Omokolade Oluwaseyi Alejolowo, Tomilola Olaolu, Rotdelmwa Filibus Maimako, Keisuke Suganuma, Yongmei Han, Kentaro Kato

Artificial cells, nanomedicine, and biotechnology　46　(sup3)　S86-S94-9　2018

DOI： 10.1080/21691401.2018.1489267 　

ISSN： 2169-1401

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The Trypanosoma and Toxoplasma spp, are etiological agents of diseases capable of causing significant morbidity, mortality and economic burden, predominantly in developing countries. Currently, there are no effective vaccines for the diseases caused by these parasites; therefore, therapy relies heavily on antiprotozoal drugs. However, the treatment options for these parasitic diseases are limited, thus underscoring the need for new anti-protozoal agents. Here, we investigated the anti-parasite action of nanoparticles. We found that the nanoparticles have strong and selective in vitro activity against T. b. brucei but moderate in vitro activity against T. congolense and T. evansi. An estimation of the in vitro anti-Trypanosoma efficacy showed that the nanoparticles had ≥200-fold selective activity against the parasite versus mammalian cells. Moreover, the nanoparticle alloys moderately suppressed the in vitro growth of T. gondii by ≥60%. In our in vivo study, the nanoparticles appeared to exhibit a trypanostatic effect, but did not totally suppress the rat parasite burden, thereby failing to appreciably extend the survival time of infected animals compared with the untreated control. In conclusion, this is the first study to demonstrate the selective in vitro anti-Trypanosoma action of nanoparticles and thus supports the potential of nanoparticles as alternative anti-parasitic agents.
Differential Gene Expression Profile of Human Neutrophils Cultured with Plasmodium falciparum-Parasitized Erythrocytes. International-journal Peer-reviewed

Terkawi MA, Takano R, Kato K

Journal of immunology research　2018　6709424-6709424　2018

DOI： 10.1155/2018/6709424 　

ISSN： 2314-8861
Modulation of host HIF-1α activity and the tryptophan pathway contributes to the anti-Toxoplasma gondii potential of nanoparticles Peer-reviewed

Oluyomi Stephen Adeyemi, Yuho Murata, Tatsuki Sugi, Yongmei Han, Kentaro Kato

Biochemistry and Biophysics Reports　11　84-92　2017/09/01
Publisher: Elsevier B.V.
DOI： 10.1016/j.bbrep.2017.07.004 　

ISSN： 2405-5808
Heparan Sulfate Proteoglycan Is an Important Attachment Factor for Cell Entry of Akabane and Schmallenberg Viruses Peer-reviewed

Shin Murakami, Akiko Takenaka-Uema, Tomoya Kobayashi, Kentaro Kato, Masayuki Shimojima, Massimo Palmarini, Taisuke Horimoto

JOURNAL OF VIROLOGY　91　(15)　2017/08

DOI： 10.1128/JVI.00503-17 　

ISSN： 0022-538X

eISSN： 1098-5514
Identification of compounds that suppress Toxoplasma gondii tachyzoites and bradyzoites Peer-reviewed

Yuho Murata, Tatsuki Sugi, Louis M. Weiss, Kentaro Kato

PLOS ONE　12　(6)　e0178203　2017/06

DOI： 10.1371/journal.pone.0178203 　

ISSN： 1932-6203
Inorganic nanoparticles kill Toxoplasma gondii via changes in redox status and mitochondrial membrane potential Peer-reviewed

Oluyomi Stephen Adeyemi, Yuho Murata, Tatsuki Sugi, Kentaro Kato

International Journal of Nanomedicine　12　1647-1661　2017/02/28

DOI： 10.2147/IJN.S122178 　

ISSN： 1178-2013 1176-9114
Involvement of beta-defensin 130 (DEFB130) in the macrophage microbicidal mechanisms for killing Plasmodium falciparum Peer-reviewed

Mohamad Alaa Terkawi, Ryo Takano, Atsushi Furukawa, Fumi Murakoshi, Kentaro Kato

SCIENTIFIC REPORTS　7　41772　2017/02

DOI： 10.1038/srep41772 　

ISSN： 2045-2322
Assessment of the growth inhibitory effect of gellan sulfate in rodent malaria in vivo Peer-reviewed

Frances C. Recuenco, Ryo Takano, Tatsuki Sugi, Hitoshi Takemae, Fumi Murakoshi, Akiko Ishiwa, Atsuko Inomata, Yukiko Enomoto-Rogers, Noreen Grace V. Fundador, Tadahisa Iwata, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato

Japanese Journal of Veterinary Research　65　(4)　207-212　2017

DOI： 10.14943/jjvr.65.4.207 　

ISSN： 0047-1917
Isolation and co-cultivation of human macrophages and neutrophils with Plasmodium falciparum-parasitized erythrocytes: An optimized system to study the phagocytic activity to malarial parasites Peer-reviewed

Mohamad Alaa Terkawi, Ryo Takano, Kentaro Kato

PARASITOLOGY INTERNATIONAL　65　(5)　545-548　2016/10

DOI： 10.1016/j.parint.2016.03.005 　

ISSN： 1383-5769
An improved method for introducing site-directed point mutation into the Toxoplasma gondii genome using CRISPR/Cas9 Peer-reviewed

Tatsuki Sugi, Kentaro Kato, Louis M. Weiss

PARASITOLOGY INTERNATIONAL　65　(5)　558-562　2016/10

DOI： 10.1016/j.parint.2016.05.002 　

ISSN： 1383-5769
Evaluation of the enzyme activity of protozoan protein kinases by using an in vitro kinase assay Peer-reviewed

Kentaro Kato

PARASITOLOGY INTERNATIONAL　65　(5)　510-513　2016/10

DOI： 10.1016/j.parint.2016.07.006 　

ISSN： 1383-5769
Pull-down method to access the cell surface receptor for Toxoplasma gondii Peer-reviewed

Haiyan Gong, Kyousuke Kobayashi, Tatsuki Sugi, Hitoshi Takemae, Taisuke Horimoto, Xuenan Xuan, Hiroomi Akashi, Kentaro Kato

PARASITOLOGY INTERNATIONAL　65　(5)　514-515　2016/10

DOI： 10.1016/j.parint.2016.08.013 　

ISSN： 1383-5769
Evaluating the use of heparin for synchronization of in vitro culture of Plasmodium falciparum Peer-reviewed

Kyousuke Kobayashi, Kentaro Kato

PARASITOLOGY INTERNATIONAL　65　(5)　549-551　2016/10

DOI： 10.1016/j.parint.2016.09.002 　

ISSN： 1383-5769
Akabane virus nonstructural protein NSm regulates viral growth and pathogenicity in a mouse model Peer-reviewed

Yukari Ishihara, Chieko Shioda, Norasuthi Bangphoomi, Keita Sugiura, Kohei Saeki, Shumpei Tsuda, Tatsuya Iwanaga, Akiko Takenaka-Uema, Kentaro Kato, Shin Murakami, Kazuyuki Uchida, Hiroomi Akashi, Taisuke Horimoto

JOURNAL OF VETERINARY MEDICAL SCIENCE　78　(9)　1391-1397　2016/09

DOI： 10.1292/jvms.16-0140 　

ISSN： 0916-7250

eISSN： 1347-7439
Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog Peer-reviewed

Kentaro Kato, Tatsuki Sugi, Hitoshi Takemae, Ryo Takano, Haiyan Gong, Akiko Ishiwa, Taisuke Horimoto, Hiroomi Akashi

PARASITES & VECTORS　9　(1)　405　2016/07

DOI： 10.1186/s13071-016-1676-1 　

ISSN： 1756-3305
Development of an improved reverse genetics system for Akabane bunyavirus Peer-reviewed

Akiko Takenaka-Uema, Keita Sugiura, Norasuthi Bangphoomi, Chieko Shioda, Kazuyuki Uchida, Kentaro Kato, Takeshi Haga, Shin Murakami, Hiroomi Akashi, Taisuke Horimoto

JOURNAL OF VIROLOGICAL METHODS　232　16-20　2016/06

DOI： 10.1016/j.jviromet.2015.12.014 　

ISSN： 0166-0934

eISSN： 1879-0984
Characterization of a recombinant Akabane mutant virus with knockout of a nonstructural protein NSs in a pregnant goat model Peer-reviewed

Akiko Takenaka-Uema, Norasuthi Bangphoomi, Chieko Shioda, Kazuyuki Uchida, Fumihiro Gen, Kentaro Kato, Takeshi Haga, Shin Murakami, Hiroomi Akashi, Taisuke Hoimoto

VIROLOGICA SINICA　31　(3)　274-277　2016/06

DOI： 10.1007/s12250-015-3704-2 　

ISSN： 1674-0769

eISSN： 1995-820X
Detection and molecular characterization of Cryptosporidium and Eimeria species in Philippine bats Peer-reviewed

Fumi Murakoshi, Frances C. Recuenco, Tsutomu Omatsu, Kaori Sano, Satoshi Taniguchi, Joseph S. Masangkay, Philip Alviola, Eduardo Eres, Edison Cosico, James Alvarez, Yumi Une, Shigeru Kyuwa, Yuki Sugiura, Kentaro Kato

PARASITOLOGY RESEARCH　115　(5)　1863-1869　2016/05

DOI： 10.1007/s00436-016-4926-4 　

ISSN： 0932-0113

eISSN： 1432-1955
Toxoplasma gondii Cyclic AMP-Dependent Protein Kinase Subunit 3 Is Involved in the Switch from Tachyzoite to Bradyzoite Development Peer-reviewed

Tatsuki Sugi, Yan Fen Ma, Tadakimi Tomita, Fumi Murakoshi, Michael S. Eaton, Rama Yakubu, Bing Han, Vincent Tu, Kentaro Kato, Shin-Ichiro Kawazu, Nishith Gupta, Elena S. Suvorova, Michael W. White, Kami Kim, Louis M. Weiss

MBIO　7　(3)　2016/05

DOI： 10.1128/mBio.00755-16 　

ISSN： 2150-7511
Dextran sulfate inhibits acute Toxoplama gondii infection in pigs Peer-reviewed

Kentaro Kato, Yuho Murata, Noriyuki Horiuchi, Atsuko Inomata, Mohamad Alaa Terkawi, Akiko Ishiwa, Yohsuke Ogawa, Shinya Fukumoto, Fumikazu Matsuhisa, Kenji Koyama

PARASITES & VECTORS　9　134　2016/03

DOI： 10.1186/s13071-016-1421-9 　

ISSN： 1756-3305
Molecular epidemiological analyses of Cryptosporidium parvum virus 1 (CSpV1), a symbiotic virus of Cryptosporidium parvum, in Japan Peer-reviewed

Fumi Murakoshi, Madoka Ichikawa-Seki, Junya Aita, Seiko Yaita, Aiko Kinami, Katsuhisa Fujimoto, Yoshifumi Nishikawa, Shin Murakami, Taisuke Horimoto, Kentaro Kato

VIRUS RESEARCH　211　69-72　2016/01

DOI： 10.1016/j.virusres.2015.09.021 　

ISSN： 0168-1702

eISSN： 1872-7492
Generation of a Recombinant Akabane Virus Expressing Enhanced Green Fluorescent Protein Peer-reviewed

Akiko Takenaka-Uema, Yousuke Murata, Fumihiro Gen, Yukari Ishihara-Saeki, Ken-ichi Watanabe, Kazuyuki Uchida, Kentaro Kato, Shin Murakami, Takeshi Haga, Hiroomi Akashi, Taisuke Horimoto

JOURNAL OF VIROLOGY　89　(18)　9477-9484　2015/09

DOI： 10.1128/JVI.00681-15 　

ISSN： 0022-538X

eISSN： 1098-5514
Heparin interacts with elongation factor 1 alpha of Cryptosporidium parvum and inhibits invasion Peer-reviewed

Atsuko Inomata, Fumi Murakoshi, Akiko Ishiwa, Ryo Takano, Hitoshi Takemae, Tatsuki Sugi, Frances Cagayat Recuenco, Taisuke Horimoto, Kentaro Kato

SCIENTIFIC REPORTS　5　11599　2015/07

DOI： 10.1038/srep11599 　

ISSN： 2045-2322
Cats as a potential source of emerging influenza virus infections Peer-reviewed

Taisuke Horimoto, Fumihiro Gen, Shin Murakami, Kiyoko Iwatsuki-Horimoto, Kentaro Kato, Masaharu Hisasue, Masahiro Sakaguchi, Chairul A. Nidom, Yoshihiro Kawaoka

VIROLOGICA SINICA　30　(3)　221-223　2015/06

DOI： 10.1007/s12250-015-3580-9 　

ISSN： 1674-0769

eISSN： 1995-820X
A single mutation in the gatekeeper residue in TgMAPKL-1 restores the inhibitory effect of a bumped kinase inhibitor on the cell cycle Peer-reviewed

Tatsuki Sugi, Shin-ichiro Kawazu, Taisuke Horimoto, Kentaro Kato

INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE　5　(1)　1-8　2015/04

DOI： 10.1016/j.ijpddr.2014.12.001 　

ISSN： 2211-3207
Macrophages Are the Determinant of Resistance to and Outcome of Nonlethal Babesia microti Infection in Mice Peer-reviewed

Mohamad Alaa Terkawi, Shinuo Cao, Maria S. Herbas, Maki Nishimura, Yan Li, Paul Franck Adjou Moumouni, Asadullah Hamid Pyarokhil, Daisuke Kondoh, Nobuo Kitamura, Yoshifumi Nishikawa, Kentaro Kato, Naoaki Yokoyama, Jinlin Zhou, Hiroshi Suzuki, Ikuo Igarashi, Xuenan Xuan

INFECTION AND IMMUNITY　83　(1)　8-16　2015/01

DOI： 10.1128/IAI.02128-14 　

ISSN： 0019-9567

eISSN： 1098-5522
The role of carbohydrates in infection strategies of enteric pathogens Peer-reviewed

Kentaro Kato, Akiko Ishiwa

Tropical Medicine and Health　43　(1)　41-52　2015
Publisher: Japanese Society of Tropical Medicine
DOI： 10.2149/tmh.2014-25 　

ISSN： 1349-4147 1348-8945
Lambda-carrageenan treatment exacerbates the severity of cerebral malaria caused by Plasmodium berghei ANKA in BALB/c mice Peer-reviewed

Frances C. Recuenco, Ryo Takano, Shiori Chiba, Tatsuki Sugi, Hitoshi Takemae, Fumi Murakoshi, Akiko Ishiwa, Atsuko Inomata, Taisuke Horimoto, Yoshiyasu Kobayashi, Noriyuki Horiuchi, Kentaro Kato

MALARIA JOURNAL　13　487　2014/12

DOI： 10.1186/1475-2875-13-487 　

ISSN： 1475-2875
Microplate assay for screening Toxoplasma gondii bradyzoite differentiation with DUAL luciferase assay Peer-reviewed

Tatsuki Sugi, Tatsunori Masatani, Fumi Murakoshi, Shin-Ichiro Kawazu, Kentaro Kato

ANALYTICAL BIOCHEMISTRY　464　9-11　2014/11

DOI： 10.1016/j.ab.2014.06.018 　

ISSN： 0003-2697

eISSN： 1096-0309
Akabane Virus Utilizes Alternative Endocytic Pathways to Entry into Mammalian Cell Lines Peer-reviewed

Norasuthi Bangphoomi, Akiko Takenaka-Uema, Tatsuki Sugi, Kentaro Kato, Hiroomi Akashi, Taisuke Horimoto

JOURNAL OF VETERINARY MEDICAL SCIENCE　76　(11)　1471-1478　2014/11

DOI： 10.1292/jvms.14-0155 　

ISSN： 0916-7250

eISSN： 1347-7439
Administration of lasalocid-NA is preventive against cryptosporidiosis of newborn calves Peer-reviewed

F. Murakoshi, M. Takeuchi, A. Inomata, T. Horimoto, M. Ito, Y. Suzuki, K. Kato

VETERINARY RECORD　175　(14)　353　2014/10

DOI： 10.1136/vr.102508 　

ISSN： 0042-4900

eISSN： 2042-7670
Analyses of the binding between Theileria orientalis major piroplasm surface proteins and bovine red blood cells Peer-reviewed

H. Takemae, T. Sugi, K. Kobayashi, F. Murakoshi, F. C. Recuenco, A. Ishiwa, A. Inomata, T. Horimoto, N. Yokoyama, K. Kato

VETERINARY RECORD　175　(6)　149　2014/08

DOI： 10.1136/vr.102535 　

ISSN： 0042-4900

eISSN： 2042-7670
Physical interaction between bovine viral diarrhea virus nonstructural protein 4A and adenosine deaminase acting on RNA (ADAR) Peer-reviewed

Yassir Mahgoub Mohamed, Norasuthi Bangphoomi, Daisuke Yamane, Yuto Suda, Kentaro Kato, Taisuke Horimoto, Hiroomi Akashi

ARCHIVES OF VIROLOGY　159　(7)　1735-1741　2014/07

DOI： 10.1007/s00705-014-1997-3 　

ISSN： 0304-8608

eISSN： 1432-8798
Interaction between Theileria orientalis 23-kDa piroplasm membrane protein and heparin Peer-reviewed

Hitoshi Takemae, Tatsuki Sugi, Kyousuke Kobayashi, Fumi Murakoshi, Frances C. Recuenco, Akiko Ishiwa, Atsuko Inomata, Taisuke Horimoto, Naoaki Yokoyama, Kentaro Kato

JAPANESE JOURNAL OF VETERINARY RESEARCH　62　(1-2)　17-24　2014/05

ISSN： 0047-1917
Characterization and binding analysis of a microneme adhesive repeat domain-containing protein from Toxoplasma gondii Peer-reviewed

Haiyan Gong, Kyousuke Kobayashi, Tatsuki Sugi, Hitoshi Takemae, Akiko Ishiwa, Frances C. Recuenco, Fumi Murakoshi, Xuenan Xuan, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato

PARASITOLOGY INTERNATIONAL　63　(2)　381-388　2014/04

DOI： 10.1016/j.parint.2013.12.006 　

ISSN： 1383-5769
Gellan sulfate inhibits Plasmodium falciparum growth and invasion of red blood cells in vitro Peer-reviewed

Frances Cagayat Recuenco, Kyousuke Kobayashi, Akiko Ishiwa, Yukiko Enomoto-Rogers, Noreen Grace V. Fundador, Tatsuki Sugi, Hitoshi Takemae, Tatsuya Iwanaga, Fumi Murakoshi, Haiyan Gong, Atsuko Inomata, Taisuke Horimoto, Tadahisa Iwata, Kentaro Kato

SCIENTIFIC REPORTS　4　4723　2014/04

DOI： 10.1038/srep04723 　

ISSN： 2045-2322
Serological evidence of infection of dogs with human influenza viruses in Japan Peer-reviewed

T. Horimoto, F. Gen, S. Murakami, K. Iwatsuki-Horimoto, K. Kato, H. Akashi, M. Hisasue, M. Sakaguchi, Y. Kawaoka, K. Maeda

VETERINARY RECORD　174　(4)　96　2014/01

DOI： 10.1136/vr.101929 　

ISSN： 0042-4900

eISSN： 2042-7670
Effects of dextran sulfates on the acute infection and growth stages of Toxoplasma gondii Peer-reviewed

Akiko Ishiwa, Kyousuke Kobayashi, Hitoshi Takemae, Tatsuki Sugi, Haiyan Gong, Frances C. Recuenco, Fumi Murakoshi, Atsuko Inomata, Taisuke Horimoto, Kentaro Kato

PARASITOLOGY RESEARCH　112　(12)　4169-4176　2013/12

DOI： 10.1007/s00436-013-3608-8 　

ISSN： 0932-0113

eISSN： 1432-1955
Analyses of Interactions Between Heparin and the Apical Surface Proteins of Plasmodium falciparum Peer-reviewed

Kyousuke Kobayashi, Ryo Takano, Hitoshi Takemae, Tatsuki Sugi, Akiko Ishiwa, Haiyan Gong, Frances C. Recuenco, Tatsuya Iwanaga, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato

SCIENTIFIC REPORTS　3　3178　2013/11

DOI： 10.1038/srep03178 　

ISSN： 2045-2322
Characterization of the interaction between Toxoplasma gondii rhoptry neck protein 4 and host cellular beta-tubulin Peer-reviewed

Hitoshi Takemae, Tatsuki Sugi, Kyousuke Kobayashi, Haiyan Gong, Akiko Ishiwa, Frances C. Recuenco, Fumi Murakoshi, Tatsuya Iwanaga, Atsuko Inomata, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato

SCIENTIFIC REPORTS　3　3199　2013/11

DOI： 10.1038/srep03199 　

ISSN： 2045-2322
Characterization of Plasmodium falciparum cdc2-related kinase and the effects of a CDK inhibitor on the parasites in erythrocytic schizogony Peer-reviewed

Tatsuya Iwanaga, Tatsuki Sugi, Kyousuke Kobayashi, Hitoshi Takemae, Haiyan Gong, Akiko Ishiwa, Fumi Murakoshi, Frances C. Recuenco, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato

PARASITOLOGY INTERNATIONAL　62　(5)　423-430　2013/10

DOI： 10.1016/j.parint.2013.05.003 　

ISSN： 1383-5769
Molecular Characterization of Cryptosporidium Isolates from Calves in Ishikari District, Hokkaido, Japan Peer-reviewed

Fumi Murakoshi, Yoriko Tozawa, Atsuko Inomata, Taisuke Horimoto, Yoshihiro Wada, Kentaro Kato

JOURNAL OF VETERINARY MEDICAL SCIENCE　75　(7)　837-840　2013/07

DOI： 10.1292/jvms.12-0435 　

ISSN： 0916-7250

eISSN： 1347-7439
Attenuation of an influenza A virus due to alteration of its hemagglutinin-neuraminidase functional balance in mice Peer-reviewed

Fumihiro Gen, Shinya Yamada, Kentaro Kato, Hiroomi Akashi, Yoshihiro Kawaoka, Taisuke Horimoto

Archives of Virology　158　(5)　1003-1011　2013

DOI： 10.1007/s00705-012-1577-3 　

ISSN： 0304-8608
Identification of mutations in TgMAPK1 of Toxoplasma gondii conferring resistance to 1NM-PP1 Peer-reviewed

Tatsuki Sugi, Kyousuke Kobayashi, Hitoshi Takemae, Haiyan Gong, Akiko Ishiwa, Fumi Murakoshi, Frances C. Recuenco, Tatsuya Iwanaga, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato

International Journal for Parasitology: Drugs and Drug Resistance　3　93-101　2013

DOI： 10.1016/j.ijpddr.2013.04.001 　

ISSN： 2211-3207
Genomic and serological detection of bat coronavirus from bats in the Philippines Peer-reviewed

Shumpei Tsuda, Shumpei Watanabe, Joseph S. Masangkay, Tetsuya Mizutani, Phillip Alviola, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hikaru Fujii, Kentaro Kato, Taisuke Horimoto, Shigeru Kyuwa, Yasuhiro Yoshikawa, Hiroomi Akashi

ARCHIVES OF VIROLOGY　157　(12)　2349-2355　2012/12

DOI： 10.1007/s00705-012-1410-z 　

ISSN： 0304-8608

eISSN： 1432-8798
Roles of Apicomplexan protein kinases at each life cycle stage Peer-reviewed

Kentaro Kato, Tatsuki Sugi, Tatsuya Iwanaga

PARASITOLOGY INTERNATIONAL　61　(2)　224-234　2012/06

DOI： 10.1016/j.parint.2011.12.002 　

ISSN： 1383-5769
Development of a method to detect viral RNA sequences from cultured cells by combining size fraction and a rapid determination system for viral RNA sequences (RDV)

Shumpei Watanabe, Tetsuya Mizutani, Kouji Sakai, Itoe Iizuka, Tomoyuki Shiota, Yusuke Sayama, Shumpei Tsuda, Kentaro Kato, Shuetsu Fukushi, Masayuki Saijo, Ichiro Kurane, Shigeru Morikawa, Hiroomi Akashi

Journal of Veterinary Science and Technology　1　(1)　103　2012

DOI： 10.4172/2157-7579.1000103 　

ISSN： 2157-7579
A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification Peer-reviewed

Haiyan Gong, Kyousuke Kobayashi, Tatsuki Sugi, Hitoshi Takemae, Hitomi Kurokawa, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato

PLOS ONE　7　(1)　e30169　2012/01

DOI： 10.1371/journal.pone.0030169 　

ISSN： 1932-6203
1NM-PP1 Treatment of Mice Infected with Toxoplasma gondii Peer-reviewed

Tatsuki Sugi, Kentaro Kato, Kyousuke Kobayashi, Hitomi Kurokawa, Hitoshi Takemae, Haiyan Gong, Frances C. Recuenco, Tatsuya Iwanaga, Taisuke Horimoto, Hiroomi Akashi

JOURNAL OF VETERINARY MEDICAL SCIENCE　73　(10)　1377-1379　2011/10

DOI： 10.1292/jvms.11-0085 　

ISSN： 0916-7250
Identification of Toxoplasma gondii cAMP Dependent Protein Kinase and Its Role in the Tachyzoite Growth Peer-reviewed

Hitomi Kurokawa, Kentaro Kato, Tatsuya Iwanaga, Tatsuki Sugi, Atsushi Sudo, Kyousuke Kobayashi, Haiyan Gong, Hitoshi Takemae, Frances C. Recuenco, Taisuke Horimoto, Hiroomi Akashi

PLOS ONE　6　(7)　e22492　2011/07

DOI： 10.1371/journal.pone.0022492 　

ISSN： 1932-6203
Equine herpesvirus 4: Recent advances using BAC technology Peer-reviewed

Walid Azab, Kentaro Kato, Azza Abdel-Gawad, Yukinobu Tohya, Hiroomi Akashi

VETERINARY MICROBIOLOGY　150　(1-2)　1-14　2011/05

DOI： 10.1016/j.vetmic.2011.01.002 　

ISSN： 0378-1135

eISSN： 1873-2542
Bovine viral diarrhea virus non-structural protein 5A interacts with NIK- and IKK beta-binding protein Peer-reviewed

Muhammad Atif Zahoor, Daisuke Yamane, Yassir Mahgoub Mohamed, Yuto Suda, Kyousuke Kobayashi, Kentaro Kato, Yukinobu Tohya, Hiroomi Akashi

JOURNAL OF GENERAL VIROLOGY　91　(Pt 8)　1939-1948　2010/08

DOI： 10.1099/vir.0.020990-0 　

ISSN： 0022-1317
Bat Coronaviruses and Experimental Infection of Bats, the Philippines Peer-reviewed

Shumpei Watanabe, Joseph S. Masangkay, Noriyo Nagata, Shigeru Morikawa, Tetsuya Mizutani, Shuetsu Fukushi, Phillip Alviola, Tsutomu Omatsu, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hikaru Fujii, Shumpei Tsuda, Maiko Endoh, Kentaro Kato, Yukinobu Tohya, Shigeru Kyuwa, Yasuhiro Yoshikawa, Hiroomi Akashi

EMERGING INFECTIOUS DISEASES　16　(8)　1217-1223　2010/08

DOI： 10.3201/eid1608.100208 　

ISSN： 1080-6040

eISSN： 1080-6059
Glycoprotein C of equine herpesvirus 4 plays a role in viral binding to cell surface heparan sulfate Peer-reviewed

Walid Azab, Koji Tsujimura, Ken Maeda, Kyousuke Kobayashi, Yassir Mahgoub Mohamed, Kentaro Kato, Tomio Matsumura, Hiroomi Akashi

VIRUS RESEARCH　151　(1)　1-9　2010/07

DOI： 10.1016/j.virusres.2010.03.003 　

ISSN： 0168-1702

eISSN： 1872-7492
Novel Betaherpesvirus in Bats Peer-reviewed

Shumpei Watanabe, Ken Maeda, Kazuo Suzuki, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hiroshi Shimoda, Kentaro Kato, Yasuhiro Yoshikawa, Shigeru Morikawa, Ichiro Kurane, Hiroomi Akashi, Tetsuya Mizutani

EMERGING INFECTIOUS DISEASES　16　(6)　986-988　2010/06

DOI： 10.3201/eid1606.091567 　

ISSN： 1080-6040

eISSN： 1080-6059
Functional analysis of Rousettus aegyptiacus "signal transducer and activator of transcription 1" (STAT1) Peer-reviewed

Hikaru Fujii, Shumpei Watanabe, Daisuke Yamane, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Kentaro Kato, Yukinobu Tohya, Shigeru Kyuwa, Yasuhiro Yoshikawa, Hiroomi Akashi

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY　34　(5)　598-602　2010/05

DOI： 10.1016/j.dci.2010.01.004 　

ISSN： 0145-305X

eISSN： 1879-0089
Use of the Kinase Inhibitor Analog 1NM-PP1 Reveals a Role for Toxoplasma gondii CDPK1 in the Invasion Step Peer-reviewed

Tatsuki Sugi, Kentaro Kato, Kyosuke Kobayashi, Shumpei Watanabe, Hitomi Kurokawa, Haiyan Gong, Kishor Pandey, Hitoshi Takemae, Hiroomi Akashi

EUKARYOTIC CELL　9　(4)　667-670　2010/04

DOI： 10.1128/EC.00351-09 　

ISSN： 1535-9778

eISSN： 1535-9786
Characterization of a thymidine kinase-deficient mutant of equine herpesvirus 4 and in vitro susceptibility of the virus to antiviral agents Peer-reviewed

Walid Azab, Koji Tsujimura, Kentaro Kato, Jun Arii, Tomomi Morimoto, Yasushi Kawaguchi, Yukinobu Tohya, Tomio Matsumura, Hiroomi Akashi

ANTIVIRAL RESEARCH　85　(2)　389-395　2010/02

DOI： 10.1016/j.antiviral.2009.11.007 　

ISSN： 0166-3542
Epizootology and experimental infection of Yokose virus in bats Peer-reviewed

Shumpei Watanabe, Tsutomu Omatsu, Mary E. G. Miranda, Joseph S. Masangkay, Naoya Ueda, Maiko Endo, Kentaro Kato, Yukinobu Tohya, Yasuhiro Yoshikawa, Hiroomi Akashi

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES　33　(1)　25-36　2010/01

DOI： 10.1016/j.cimid.2008.07.008 　

ISSN： 0147-9571
Plasmodium falciparum BAEBL Binds to Heparan Sulfate Proteoglycans on the Human Erythrocyte Surface Peer-reviewed

Kyousuke Kobayashi, Kentaro Kato, Tatsuki Sugi, Hitoshi Takemae, Kishor Pandey, Haiyan Gong, Yukinobu Tohya, Hiroomi Akashi

JOURNAL OF BIOLOGICAL CHEMISTRY　285　(3)　1716-1725　2010/01

DOI： 10.1074/jbc.M109.021576 　

ISSN： 0021-9258

eISSN： 1083-351X
Characterization of Plasmodium falciparum calcium-dependent protein kinase 4 Peer-reviewed

Kentaro Kato, Atsushi Sudo, Kyousuke Kobayashi, Tatsuki Sugi, Yukinobu Tohya, Hiroomi Akashi

PARASITOLOGY INTERNATIONAL　58　(4)　394-400　2009/12

DOI： 10.1016/j.parint.2009.08.001 　

ISSN： 1383-5769
Molecular analyses of Toxoplasma gondii calmodulin-like domain protein kinase isoform 3 Peer-reviewed

Tatsuki Sugi, Kentaro Kato, Kyousuke Kobayashi, Kishor Pandey, Hitoshi Takemae, Hitomi Kurokawa, Yukinobu Tohya, Hiroomi Akashi

PARASITOLOGY INTERNATIONAL　58　(4)　416-423　2009/12

DOI： 10.1016/j.parint.2009.08.005 　

ISSN： 1383-5769
Characterization and application of monoclonal antibodies to bovine viral diarrhea virus nonstructural protein 5A Peer-reviewed

Muhammad Atif Zahoor, Daisuke Yamane, Yassir Mahgoub Mohamed, Kyousuke Kobayashi, Kentaro Kato, Yukinobu Tohya, Hiroomi Akashi

ARCHIVES OF VIROLOGY　154　(11)　1745-1754　2009/11

DOI： 10.1007/s00705-009-0505-7 　

ISSN： 0304-8608

eISSN： 1432-8798
Activation of extracellular signal-regulated kinase in MDBK cells infected with bovine viral diarrhea virus Peer-reviewed

Daisuke Yamane, Muhammad Atif Zahoor, Yassir Mahgoub Mohamed, Walid Azab, Kentaro Kato, Yukinobu Tohya, Hiroomi Akashi

ARCHIVES OF VIROLOGY　154　(9)　1499-1503　2009/09

DOI： 10.1007/s00705-009-0453-2 　

ISSN： 0304-8608
Detection of a new bat gammaherpesvirus in the Philippines Peer-reviewed

Shumpei Watanabe, Naoya Ueda, Koichiro Iha, Joseph S. Masangkay, Hikaru Fujii, Phillip Alviola, Tetsuya Mizutani, Ken Maeda, Daisuke Yamane, Azab Walid, Kentaro Kato, Shigeru Kyuwa, Yukinobu Tohya, Yasuhiro Yoshikawa, Hiroomi Akashi

VIRUS GENES　39　(1)　90-93　2009/08

DOI： 10.1007/s11262-009-0368-8 　

ISSN： 0920-8569

eISSN： 1572-994X
Analysis of herpesvirus host specificity determinants using herpesvirus genomes as bacterial artificial chromosomes Peer-reviewed

Jun Arii, Kentaro Kato, Yasushi Kawaguchi, Yukinobu Tohya, Hiroomi Akashi

MICROBIOLOGY AND IMMUNOLOGY　53　(8)　433-441　2009/08

DOI： 10.1111/j.1348-0421.2009.00147.x 　

ISSN： 0385-5600
Application of retrovirus-mediated expression cloning for receptor screening of a parasite Peer-reviewed

Kyousuke Kobayashi, Kentaro Kato, Tatsuki Sugi, Daisuke Yamane, Masayuki Shimojima, Yukinobu Tohya, Hiroomi Akashi

ANALYTICAL BIOCHEMISTRY　389　(1)　80-82　2009/06

DOI： 10.1016/j.ab.2009.03.008 　

ISSN： 0003-2697

eISSN： 1096-0309
Microarray analysis reveals distinct signaling pathways transcriptionally activated by infection with bovine viral diarrhea virus in different cell types Peer-reviewed

Daisuke Yamane, Muhammad Atif Zahoor, Yassir Mahgoub Mohamed, Walid Azab, Kentaro Kato, Yukinobu Tohya, Hiroomi Akashi

VIRUS RESEARCH　142　(1-2)　188-199　2009/06

DOI： 10.1016/j.virusres.2009.02.015 　

ISSN： 0168-1702
Inhibition of Sphingosine Kinase by Bovine Viral Diarrhea Virus NS3 Is Crucial for Efficient Viral Replication and Cytopathogenesis Peer-reviewed

Daisuke Yamane, Muhammad A. Zahoor, Yassir M. Mohamed, Walid Azab, Kentaro Kato, Yukinobu Tohya, Hiroomi Akashi

JOURNAL OF BIOLOGICAL CHEMISTRY　284　(20)　13648-13659　2009/05

DOI： 10.1074/jbc.M807498200 　

ISSN： 0021-9258
Cloning of the genome of equine herpesvirus 4 strain TH20p as an infectious bacterial artificial chromosome Peer-reviewed

Walid Azab, Kentaro Kato, Jun Arii, Koji Tsujimura, Daisuke Yamane, Yukinobu Tohya, Tomio Matsumura, Hiroomi Akashi

ARCHIVES OF VIROLOGY　154　(5)　833-842　2009/05

DOI： 10.1007/s00705-009-0382-0 　

ISSN： 0304-8608
CHARACTERIZATION OF PLASMODIUM FALCIPARUM PROTEIN KINASE 2 Peer-reviewed

Kentaro Kato, Atsushi Sudo, Kyousuke Kobayashi, Yukinobu Tohya, Hiroomi Akashi

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE　79　(6)　122-122　2008/12

DOI： 10.1016/j.parint.2009.08.001 　

ISSN： 0002-9637
Characterization of Plasmodium falciparum protein kinase 2 Peer-reviewed

Kentaro Kato, Atsushi Sudo, Kyousuke Kobayashi, Yukinobu Tohya, Hiroomi Akashi

MOLECULAR AND BIOCHEMICAL PARASITOLOGY　162　(1)　87-95　2008/11

DOI： 10.1016/j.molbiopara.2008.07.007 　

ISSN： 0166-6851
Ligation-mediated amplification for effective rapid determination of viral RNA sequences (RDV) Peer-reviewed

Shumpei Watanabe, Tetsuya Mizutani, Kouji Sakai, Kentaro Kato, Yukinobu Tohya, Shuetsu Fukushi, Masayuki Saijo, Yasuhiro Yoshikawa, Ichiro Kurane, Shigeru Morikawa, Hiroomi Akashi

JOURNAL OF CLINICAL VIROLOGY　43　(1)　56-59　2008/09

DOI： 10.1016/j.jcv.2008.05.004 　

ISSN： 1386-6532
Susceptibility of Plasmodium falciparum cyclic AMP-dependent protein kinase and its mammalian homologue to the inhibitors Peer-reviewed

Atsushi Sudo, Kentaro Kato, Kyousuke Kobayashi, Yukinobu Tohya, Hiroomi Akashi

MOLECULAR AND BIOCHEMICAL PARASITOLOGY　160　(2)　138-142　2008/08

DOI： 10.1016/j.molbiopara.2008.03.011 　

ISSN： 0166-6851
The relationship between the viral RNA level and upregulation of innate immunity in spleen of cattle persistently infected with bovine viral diarrhea virus Peer-reviewed

Daisuke Yamane, Kentaro Kato, Yukinobu Tohya, Hiroorni Akashi

VETERINARY MICROBIOLOGY　129　(1-2)　69-79　2008/05

DOI： 10.1016/j.vetmic.2007.11.004 　

ISSN： 0378-1135
Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I Peer-reviewed

Yohsuke Ogawa, Keita Sugiura, Kentaro Kato, Yukinobu Tohya, Hiroomi Akashi

JOURNAL OF GENERAL VIROLOGY　88　(Pt 12)　3385-3390　2007/12

DOI： 10.1099/vir.0.83173-0 　

ISSN： 0022-1317
Characterization of temperature-sensitive Akabane virus mutants and their roles in attenuation Peer-reviewed

Y. Ogawa, K. Kato, Y. Tohya, H. Akashi

ARCHIVES OF VIROLOGY　152　(9)　1679-1686　2007/09

DOI： 10.1007/s00705-007-0991-4 　

ISSN： 0304-8608
Comparison of Akabane virus isolated from sentinel cattle in Japan Peer-reviewed

Yohsuke Ogawa, Toyoko Fukutomi, Keita Sugiura, Katsuaki Sugiura, Kentaro Kato, Yukinobu Tohya, Hiroomi Akashi

VETERINARY MICROBIOLOGY　124　(1-2)　16-24　2007/09

DOI： 10.1016/j.vetmic.2007.03.020 　

ISSN： 0378-1135
Sequence determination and functional analysis of the Akabane virus (family Bunyaviridae) L RNA segment Peer-reviewed

Y. Ogawa, K. Kato, Y. Tohya, H. Akashi

ARCHIVES OF VIROLOGY　152　(5)　971-979　2007/05

DOI： 10.1007/s00705-006-0912-y 　

ISSN： 0304-8608
The double-stranded RNA-induced apoptosis pathway is involved in the cytopathogenicity of cytopathogenic Bovine viral diarrhea virus Peer-reviewed

Daisuke Yamane, Kentaro Kato, Yukinobu Tohya, Hiroomi Akashi

JOURNAL OF GENERAL VIROLOGY　87　(Pt 10)　2961-2970　2006/10

DOI： 10.1009/vir.0.81820-0 　

ISSN： 0022-1317
Genetic analysis of calicivirus genomes detected in intestinal contents of piglets in Japan Peer-reviewed

Y. Yin, Y. Tohya, Y. Ogawa, D. Numazawa, K. Kato, H. Akashi

ARCHIVES OF VIROLOGY　151　(9)　1749-1759　2006/09

DOI： 10.1007/s00705-006-0750-y 　

ISSN： 0304-8608
Epstein-Barr virus protein kinase BGLF4 is a virion tegument protein that dissociates from virions in a phosphorylation-dependent process and phosphorylates the viral immediate-early protein BZLF1 Peer-reviewed

R Asai, A Kato, K Kato, M Kanamori-Koyama, K Sugimoto, T Sairenji, Y Nishiyama, Y Kawaguchi

JOURNAL OF VIROLOGY　80　(11)　5125-5134　2006/06

DOI： 10.1128/JVI.02674-05 　

ISSN： 0022-538X
Junctional adhesion molecule 1 is a functional receptor for feline calicivirus Peer-reviewed

A Makino, M Shimojima, T Miyazawa, K Kato, Y Tohya, H Akashi

JOURNAL OF VIROLOGY　80　(9)　4482-4490　2006/05

DOI： 10.1128/JVI.80.9.4482-4490.2006 　

ISSN： 0022-538X
Construction of an infectious clone of canine herpesvirus genome as a bacterial artificial chromosome Peer-reviewed

J Arii, O Hushur, K Kato, Y Kawaguchi, Y Tohya, H Akashi

MICROBES AND INFECTION　8　(4)　1054-1063　2006/04

DOI： 10.1016/j.micinf.2005.11.004 　

ISSN： 1286-4579
Domain III of Plasmodium falciparum apical membrane antigen 1 binds to the erythrocyte membrane protein Kx Peer-reviewed

K Kato, DCG Mayer, S Singh, M Reid, LH Miller

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　102　(15)　5552-5557　2005/04

DOI： 10.1073/pnas.0501594102 　

ISSN： 0027-8424
Plasmodium falciparum cysteine protease falcipain-1 is not essential in erythrocytic stage malaria parasites Peer-reviewed

PS Sijwali, K Kato, KB Seydel, J Gut, J Lehman, M Klemba, DE Goldberg, LH Miller, PJ Rosenthal

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　101　(23)　8721-8726　2004/06

DOI： 10.1073/pnas.0402738101 　

ISSN： 0027-8424
Identification of protein kinases responsible for phosphorylation of Epstein-Barr virus nuclear antigen leader protein at serine-35, which regulates its coactivator function Peer-reviewed

K Kato, A Yokoyama, Y Tohya, H Akashi, Y Nishiyama, Y Kawaguchi

JOURNAL OF GENERAL VIROLOGY　84　(Pt 12)　3381-3392　2003/12

DOI： 10.1099/vir.0.19454-0 　

ISSN： 0022-1317
Protein kinases conserved in herpesviruses potentially share a function mimicking the cellular protein kinase cdc2 Peer-reviewed

Y Kawaguchi, K Kato

REVIEWS IN MEDICAL VIROLOGY　13　(5)　331-340　2003/09

DOI： 10.1002/rmv.402 　

ISSN： 1052-9276
CD56 expression in feline lymphoid cells Peer-reviewed

M Shimojima, Y Nishimura, T Miyazawa, K Kato, Y Tohya, H Akashi

JOURNAL OF VETERINARY MEDICAL SCIENCE　65　(7)　769-773　2003/07

DOI： 10.1292/jvms.65.769 　

ISSN： 0916-7250
Establishment of a GFP-based indicator cell line to quantitate feline foamy virus Peer-reviewed

HTT Phung, Y Tohya, M Shimojima, K Kato, T Miyazawa, H Akashi

JOURNAL OF VIROLOGICAL METHODS　109　(2)　125-131　2003/05

DOI： 10.1016/S0166-0934(03)00062-4 　

ISSN： 0166-0934
Conserved protein kinases encoded by herpesviruses and cellular protein kinase cdc2 target the same phosphorylation site in eukaryotic elongation factor 1 delta Peer-reviewed

Y Kawaguchi, K Kato, M Tanaka, M Kanamori, Y Nishiyama, Y Yamanashi

JOURNAL OF VIROLOGY　77　(4)　2359-2368　2003/02

DOI： 10.1128/JVI.77.4.2359-2368.2003 　

ISSN： 0022-538X
Identification and characterization of Marek's disease virus serotype 1 (MDV1) ICP22 gene product: MDV1 ICP22 transactivates the MDV1 ICP27 promoter synergistically with MDV1 ICP4 Peer-reviewed

K Kato, Y Izumiya, Y Tohya, E Takahashi, K Hirai, Y Kawaguchi

VETERINARY MICROBIOLOGY　85　(4)　305-313　2002/04

DOI： 10.1016/S0378-1135(01)00522-3 　

ISSN： 0378-1135
A feline CD2 homologue interacts with human red blood cells Peer-reviewed

M Shimojima, Y Nishimura, T Miyazawa, K Kato, K Nakamura, Y Izumiya, H Akashi, Y Tohya

IMMUNOLOGY　105　(3)　360-366　2002/03

DOI： 10.1046/j.0019-2805.2001.01371.x 　

ISSN： 0019-2805

eISSN： 1365-2567
Conserved region CR2 of Epstein-Barr virus nuclear antigen leader protein is a multifunctional domain that mediates self-association as well as nuclear localization and nuclear matrix association Peer-reviewed

M Tanaka, A Yokoyama, M Igarashi, G Matsuda, K Kato, M Kanamori, K Hirai, Y Kawaguchi, Y Yamanashi

JOURNAL OF VIROLOGY　76　(3)　1025-1032　2002/02

DOI： 10.1128/JVI.76.3.1025-1032.2002 　

ISSN： 0022-538X
Identification of major phosphorylation sites of Epstein-Barr virus nuclear antigen leader protein (EBNA-LP): Ability of EBNA-LP to induce latent membrane protein 1 cooperatively with EBNA-2 is regulated by phosphorylation Peer-reviewed

A Yokoyama, M Tanaka, G Matsuda, K Kato, M Kanamori, H Kawasaki, H Hirano, Kitabayashi, I, M Ohki, K Hirai, Y Kawaguchi

JOURNAL OF VIROLOGY　75　(11)　5119-5128　2001/06

DOI： 10.1128/JVI.75.11.5119-5128.2001 　

ISSN： 0022-538X

eISSN： 1098-5514
Epstein-Barr virus-encoded protein kinase BGLF4 mediates hyperphosphorylation of cellular elongation factor 1 delta (EF-1 delta): EF-1 delta is universally modified by conserved protein kinases of herpesviruses in mammalian cells Peer-reviewed

K Kato, Y Kawaguchi, M Tanaka, M Igarashi, A Yokoyama, G Matsuda, M Kanamori, K Nakajima, Y Nishimura, M Shimojima, HTT Phung, E Takahashi, K Hirai

JOURNAL OF GENERAL VIROLOGY　82　(Pt 6)　1457-1463　2001/06

DOI： 10.1099/0022-1317-82-6-1457 　

ISSN： 0022-1317
Herpes simplex virus 1 alpha regulatory protein ICP0 functionally interacts with cellular transcription factor BMAL1 Peer-reviewed

Y Kawaguchi, M Tanaka, A Yokoymama, G Matsuda, K Kato, H Kagawa, K Hirai, B Roizman

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　98　(4)　1877-1882　2001/02

DOI： 10.1073/pnas.041592598 　

ISSN： 0027-8424
Characterization of the regulatory function of the Marek's disease virus type-1 ICP22 homolog Peer-reviewed

K Kato, Y Kawaguchi, Y Izumiya, Y Tohya, E Takahashi, K Hirai

CURRENT PROGRESS ON MAREK'S DISEASE RESEARCH　219-223　2001
Interaction of Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) with HS1-associated protein X-1: Implication of cytoplasmic function of EBNA-LP Peer-reviewed

Y Kawaguchi, K Nakajima, M Igarashi, T Morita, M Tanaka, M Suzuki, A Yokoyama, G Matsuda, K Kato, M Kanamori, K Hirai

JOURNAL OF VIROLOGY　74　(21)　10104-10111　2000/11

DOI： 10.1128/JVI.74.21.10104-10111.2000 　

ISSN： 0022-538X
Identification of the Marek's Disease Virus Serotype 2 Genes Homologous to the Glycoprotein B (UL27), ICP18.5 (UL28) and Major DNA-Binding Protein (UL29) Genes of Herpes Simplex Virus Type 1 Peer-reviewed

Kentaro Kato, Hyung-Kwan Jang, Yoshihiro Izumiya, Jin-Shun Cai, Yoshinori Tsushima, Takayuki Miyazawa, Chieko Kai, Takeshi Mikami

Journal of Veterinary Medical Science　61　(10)　1161-1165　1999/10

ISSN： 1782-155X
Identification of the Marek's disease virus serotype 2 genes homologous to the glycoprotein B (UL27), ICP18.5 (UL28) and major DNA-binding protein (UL29) genes of herpes simplex virus type 1 Peer-reviewed

K Kato, HK Jang, Y Izumiya, JS Cai, Y Tsushima, T Miyazawa, CK Kai, T Mikami

JOURNAL OF VETERINARY MEDICAL SCIENCE　61　(10)　1161-1165　1999/10

DOI： 10.1292/jvms.61.1161 　

ISSN： 0916-7250
Identification and Sequence Analysis of the Marek's Disease Virus Serotype 2 Homologous Genes of the Herpes Simplex Virus Type 1 UL25, UL26 and UL26.5 Genes Peer-reviewed

Kentaro Kato, Hyung-Kwan Jang, Yoshihiro Izumiya, Jin-Shun Cai, Yoshinori Tsushima, Takayuki Miyazawa, Chieko Kai, Takeshi Mikami

Journal of Veterinary Medical Science　61　(7)　787-793　1999/07

ISSN： 1782-155X
Identification and sequence analysis of the Marek's disease virus serotype 2 homologous genes of the herpes simplex virus type 1 UL25, UL26 and UL26.5 genes Peer-reviewed

K Kato, HK Jang, Y Izumiya, JS Cai, Y Tsushima, T Miyazawa, C Kai, T Mikami

JOURNAL OF VETERINARY MEDICAL SCIENCE　61　(7)　787-793　1999/07

DOI： 10.1292/jvms.61.787 　

ISSN： 0916-7250
Identification and DNA sequence analysis of the Marek's disease virus serotype 2 genes homologous to the herpes simplex virus type 1 UL20 and UL21 Peer-reviewed

Shinichi Hatama, Hyung-Kwan Jang, Yoshihiro Izumiya, Jin-Shun Cai, Yoshinori Tsushima, Kentaro Kato, Takayuki Miyazawa, Chieko Kai, Eiji Takahashi, Takeshi Mikami

Journal of Veterinary Medical Science　61　(6)　587-593　1999/06

ISSN： 1782-155X
Identification and sequence analysis of the Marek's disease virus serotype 2 gene homologous to the herpes simplex virus type 1 UL52 protein Peer-reviewed

Yoshihiro Izumiya, Hyung-Kwan Jang, Jin-Shun Cai, Yorihiro Nishimura, Kazuya Nakamura, Yoshinori Tsushima, Kentaro Kato, Takayuki Miyazawa, Chieko Kai, Takeshi Mikami

Journal of Veterinary Medical Science　61　(6)　683-687　1999/06

ISSN： 1782-155X
Identification and DNA sequence analysis of the Marek's disease virus serotype 2 genes homologous to the herpes simplex virus type 1 UL20 and UL21 Peer-reviewed

S Hatama, HK Jang, Y Izumiya, JS Cai, Y Tsushima, K Kato, T Miyazawa, C Kai, E Takahashi, T Mikami

JOURNAL OF VETERINARY MEDICAL SCIENCE　61　(6)　587-593　1999/06

DOI： 10.1292/jvms.61.587 　

ISSN： 0916-7250
Identification and sequence analysis of the Marek's disease virus serotype 2 gene homologous to the herpes simplex virus type 1 UL52 protein Peer-reviewed

Y Izumiya, HK Jang, JS Cai, Y Nishimura, K Nakamura, Y Tsushima, K Kato, T Miyazawa, C Kai, T Mikami

JOURNAL OF VETERINARY MEDICAL SCIENCE　61　(6)　683-687　1999/06

DOI： 10.1292/jvms.61.683 　

ISSN： 0916-7250
Identification and Structure of the Marek's Disease Virus Serotype 2 Glycoprotein M Gene: Comparison with Glycoprotein M Genes of Herpesviridae Family Peer-reviewed

Jin-Shun Cai, Hyung-Kwan Jang, Yoshihiro Izumiya, Yoshinori Tsushima, Kentaro Kato, Armando M. Damiani, Takayuki Miyazawa, Chieko Kai, Eiji Takahashi, Takeshi Mikami

Journal of Veterinary Medical Science　61　(5)　503-511　1999/05

ISSN： 1782-155X
Identification and structure of the Marek's disease virus serotype 2 glycoprotein M gene: Comparison with glycoprotein M genes of Herpesviridae family Peer-reviewed

JS Cai, HK Jang, Y Izumiya, Y Tsushima, K Kato, AM Damiani, T Miyazawa, C Kai, E Takahashi, T Mikami

JOURNAL OF VETERINARY MEDICAL SCIENCE　61　(5)　503-511　1999/05

DOI： 10.1292/jvms.61.503 　

ISSN： 0916-7250
Gene arrangement and RNA transcription of the BamHI fragments K and M2 within the non-oncogenic Marek's disease virus serotype 2 unique long genome region Peer-reviewed

Y Tsushima, HK Jang, Y Izumiya, JS Cai, K Kato, T Miyazawa, C Kai, E Takahashi, T Mikami

VIRUS RESEARCH　60　(1)　101-110　1999/03

DOI： 10.1016/S0168-1702(99)00010-6 　

ISSN： 0168-1702
Identification and transcriptional analysis of the homologues of the herpes simplex virus type 1 UL41 to UL51 genes in the genome of nononcogenic Marek's disease virus serotype 2 Peer-reviewed

Y Izumiya, HK Jang, H Kashiwase, JS Cai, Y Nishimura, Y Tsushima, K Kato, T Miyazawa, C Kai, T Mikami

JOURNAL OF GENERAL VIROLOGY　79　1997-2001　1998/08

DOI： 10.1099/0022-1317-79-8-1997 　

ISSN： 0022-1317

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Misc. 34

Identification of potent drug candidates among the traditional Chinese medicine compounds for the treatment of Cryptosporidiosis

ビンカビルモハメドハッジャジュ, ビンカビルモハメドハッジャジュ, レクエンコフランセスカガヤット, モハメドジンヌルカティージャ, 渡邊仁奈, 渡邉謙一, 福田康弘, 伴戸寛徳, 暮地本宙己, 玄学南, 加藤健太郎

日本寄生虫学会大会プログラム・抄録集　91st　2022

ISSN： 1348-4613
Development of a Highly Sensitive Method for the Detection of Cryptosporidium parvum Virus Type 1 (CSpV1)

SHEHATA Ayman Ahmed, BANDO Hironori, FUKUDA Yasuhiro, KABIR Mohammad Hazzaz Bin, MURAKOSHI Fumi, ITOH Megumi, FUJIKURA Atsushi, OKAWA Hiroaki, ENDO Takuto, GOTO Akira, KACHI Masayuki, NAKAYAMA Toshie, KANO Yuto, OISHI Shoko, OTOMARU Konosuke, KAZAMA Kei, ESSA Mohamed Ibrahim, KATO Kentaro

Journal of Integrated Field Science　(17)　39-39　2020/03
Publisher: Field Science Center, Graduate School of Agricultural Science, Tohoku University
ISSN： 2434-4761

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Poster Sessions
Development of a highly sensitive method for the detection of Cryptosporidium parvum virus type 1 (CSpV1)

SHEHATA Ayman Ahmed, SHEHATA Ayman Ahmed, 伴戸寛徳, 福田康弘, KABIR Mohammad Hazzaz Bin, 村越ふみ, 伊藤めぐみ, ESSA Mohamed Ibrahim, 加藤健太郎, 加藤健太郎

日本寄生虫学会大会プログラム・抄録集　89th　2020

ISSN： 1348-4613
クリプトスポリジウムにおけるfeeder organelle微細構造の立体的観察

日本獣医学会学術集会講演要旨集　162nd　357-357　2019/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
酪農場における子牛のクリプトスポリジウム排出数の推移と糞便性状の関連

村越ふみ, 下タ村幸薫, 伊藤めぐみ, 滄木孝弘, 山岸則夫, 加藤健太郎, 芝野健一

北海道獣医師会雑誌　60　(8)　357-357　2016/08
Publisher: (公社)北海道獣医師会
ISSN： 0018-3385
フィリピンおよび日本のコウモリから検出されたクリプトスポリジウムおよびアイメリアの新規ジェノタイプ

村越ふみ, 小山憲司, 大松勉, 谷口怜, 宇根有美, 赤坂卓美, 堀内雅之, MASANGKAY Joseph, 久和茂, 加藤健太郎

日本寄生虫学会大会プログラム・抄録集　85th　55　2016/02

ISSN： 1348-4613
クリプトスポリジウム原虫の新規分子マーカーと感染の分子機構

村越ふみ, 加藤健太郎

医学のあゆみ　259　(4)　2016

ISSN： 0039-2359
アカバネウイルス感染におけるヘパラン硫酸プロテオグリカンの役割

村上晋, 上間亜希子, 加藤健太郎, 加藤健太郎, 加藤健太郎, 堀本泰介

日本獣医学会学術集会講演要旨集　158th　2015

ISSN： 1347-8621
DUAL‐LUCIFERASEを利用したトキソプラズマ潜伏感染の高効率評価系

杉達紀, 正谷達謄, 村越ふみ, 河津信一郎, 加藤健太郎

日本獣医学会学術集会講演要旨集　157th　357-357　2014/08/11
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
GFP発現組換えアカバネウイルスの作出

上間亜希子, 村田洋介, 石原ゆかり, 渡邉謙一, 内田和幸, 加藤健太郎, 村上晋, 芳賀猛, 堀本泰介, 明石博臣

日本獣医学会学術集会講演要旨集　157th　393　2014/08/11

ISSN： 1347-8621
アカバネウイルスGcタンパク質の膜融合能に関与するアミノ酸の同定

村上晋, 大塚旭, 上間亜希子, 加藤健太郎, 加藤健太郎, 堀本泰介

日本獣医学会学術集会講演要旨集　157th　2014

ISSN： 1347-8621
ヤギにおける組換えアカバネウイルスワクチン候補株の安全性と有効性の検討

上間亜希子, 杉浦慶太, 杉浦慶太, BANGPHOOMI Norasuthi, BANGPHOOMI Norasuthi, 塩田知恵子, 内田和幸, 加藤健太郎, 加藤健太郎, 芳賀猛, 堀本泰介, 明石博臣

日本獣医学会学術集会講演要旨集　157th　2014

ISSN： 1347-8621
GFP発現組換えアカバネウイルスの作出

上間亜希子, 村田洋介, 石原ゆかり, 石原ゆかり, 渡邊謙一, 内田和幸, 加藤健太郎, 加藤健太郎, 村上晋, 芳賀猛, 堀本泰介, 明石博臣

日本ウイルス学会学術集会プログラム・抄録集　62nd　2014
小型ピロプラズマ主要膜抗原と結合する宿主因子の解析

竹前等, 杉達紀, 高野量, 村越ふみ, RECUENCO Frances, 石和玲子, 堀本泰介, 横山直明, 加藤健太郎

日本獣医学会学術集会講演要旨集　156th　238　2013/08/30

ISSN： 1347-8621
石狩管内における飼育牛のクリプトスポリジウム原虫の分子疫学調査

戸澤世利子, 和田好洋, 村越ふみ, 堀本泰介, 加藤健太郎

北海道獣医師会雑誌　57　(8)　2013

ISSN： 0018-3385
わが国の哺乳動物におけるインフルエンザウイルス感染

堀本泰介, 玄文宏, 岩附研子, 木曽真紀, 村上晋, 加藤健太郎, 久末正晴, 阪口雅弘, 明石博臣, 伊藤壽啓, 河岡義裕, 前田健

日本ウイルス学会学術集会プログラム・抄録集　60th　247-247　2012/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
NA発現細胞で高い増殖性を示すインフルエンザウイルスHA変異株の性状解析

玄文宏, 山田晋弥, 岩附研子, 加藤健太郎, 明石博臣, 河岡義裕, 堀本泰介

日本ウイルス学会学術集会プログラム・抄録集　60th　2012
フィリピンに生息するコウモリのコロナウイルス保有状況の調査

津田峻平, 渡辺俊平, MASANGKAY Joseph S, 水谷哲也, ALVIOLA Phillip, 上田直也, 伊波興一朗, 谷口怜, 藤井ひかる, 加藤健太郎, 堀本泰介, 久和茂, 吉川泰弘, 明石博臣

日本獣医学会学術集会講演要旨集　152nd　243　2011/08/31

ISSN： 1347-8621
Toxoplasma gondii PKA regulatory subunitの機能解析

黒川瞳, 加藤健太郎, 岩永達也, 杉達紀, 首藤篤史, 小林郷介, ゴン海燕, 竹前等, 堀本泰介, 明石博臣

日本獣医学会学術集会講演要旨集　152nd　2011

ISSN： 1347-8621
フィリピンにおけるコウモリコロナウイルスの検出および飼育食果コウモリを用いたウイルス感染実験

渡辺俊平, MASANGKAY Josenh S, 永田典代, 森川茂, 水谷哲也, 福士秀悦, 大松勉, 上田直也, 伊波興一朗, 谷口怜, 藤井ひかる, 津田峻平, 加藤健太郎, 遠矢幸伸, 久和茂, 吉川泰弘, 明石博臣

日本ウイルス学会学術集会プログラム・抄録集　58th　362　2010/10/15
網羅的検出手法によるコウモリ保有ウイルスの探索

渡辺俊平, 水谷哲也, 前田健, 大松勉, 鈴木和男, 永田典代, 森川茂, 加藤健太郎, 遠矢幸伸, 久和茂, 吉川泰弘, 明石博臣

日本獣医学会学術集会講演要旨集　149回　89-89　2010/03
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
トキソプラズマ原虫のcAMP依存性プロテインキナーゼの機能解析

黒川瞳, 加藤健太郎, 首藤篤史, 小林郷介, 杉達紀, 竹前等, ゴン海燕, 明石博臣

日本獣医学会学術集会講演要旨集　150th　2010

ISSN： 1347-8621
エジプトルーセットオオコウモリにおけるSTAT1のクローニングとその機能解析

藤井ひかる, 渡辺俊平, 上田直也, 伊波興一朗, 谷口怜, 加藤健太郎, 遠矢幸伸, 久和茂, 吉川泰弘, 明石博臣

日本ウイルス学会学術集会プログラム・抄録集　57th　462　2009/10/01
Plasmodium falciparum BAEBLとヘパラン硫酸プロテオグリカンの相互作用に関する研究

小林郷介, 加藤健太郎, 首藤篤史, 杉達紀, 下島昌幸, 遠矢幸伸, 明石博臣

日本寄生虫学会大会プログラム・抄録集　78th　2009

ISSN： 1348-4613
改良RDV法を用いたコウモリ由来新規βヘルペスウイルスの同定

渡辺俊平, 前田健, 水谷哲也, 鈴木和男, 藤井ひかる, 上田直也, 伊波興一朗, 谷口怜, 加藤健太郎, 遠矢幸伸, 久和茂, 吉川泰弘, 明石博臣

日本獣医学会学術集会講演要旨集　148th　198-198　2009
Publisher: (公社)日本獣医学会
ISSN： 1347-8621
エジプトルーセットオオコウモリにおけるSTAT1のクローニングとその機能解析

藤井ひかる, 渡辺俊平, 上田直也, 伊波興一朗, 谷口怜, 加藤健太郎, 遠矢幸伸, 久和茂, 吉川泰弘, 明石博臣

日本獣医学会学術集会講演要旨集　148th　193　2009

ISSN： 1347-8621
宿主移動に伴うマラリア原虫プロテインキナーゼPfCDPK4の機能変化

加藤健太郎, 首藤篤史, 小林郷介, 杉達紀, 遠矢幸伸, 明石博臣

日本獣医学会学術集会講演要旨集　146th　2008

ISSN： 1347-8621
Plasmodium falciparum BAEBLは赤血球表面のヘパラン硫酸プロテオグリカンに結合する

小林郷介, 加藤健太郎, 首藤篤史, 杉達紀, 下島昌幸, 遠矢幸伸, 明石博臣

日本獣医学会学術集会講演要旨集　146th　2008

ISSN： 1347-8621
熱帯熱マラリア原虫プロテインキナーゼPfCDPK4のリン酸化の感染環における役割

加藤健太郎, 首藤篤史, 小林郷介, 杉達紀, 遠矢幸紳, 明石博臣

日本寄生虫学会大会プログラム・抄録集　77th　2008

ISSN： 1348-4613
マラリア原虫プロテインキナーゼPfPK2の機能解析

加藤健太郎, 首藤篤史, 小林郷介, 遠矢幸伸, 明石博臣

日本獣医学会学術集会講演要旨集　144th　2007

ISSN： 1347-8621
Plasmodium falciparum protein kinase2(Pfpk2)の機能解析

加藤健太郎, 首藤篤史, 小林郷介, 遠矢幸伸, 明石博臣

日本寄生虫学会大会プログラム・抄録集　76th　2007

ISSN： 1348-4613
マラリア原虫のcAMP-dependent protein kinaseの機能解析

首藤篤史, 加藤健太郎, 小林郷介, 遠矢幸伸, 明石博臣

日本獣医学会学術集会講演要旨集　144th　2007

ISSN： 1347-8621
熱帯熱マラリア原虫のcAMP-dependent protein kinase(PfPKA)の機能解析

首藤篤史, 加藤健太郎, 小林郷介, 遠矢幸伸, 明石博臣

日本寄生虫学会大会プログラム・抄録集　76th　2007

ISSN： 1348-4613
ブタ腸管由来カリシウイルスの遺伝子検索と性状解析

尹益哲, 小川洋介, 加藤健太郎, 遠矢幸伸, 片山和彦, 武田直和, 山下修一, 明石博臣

日本獣医学会学術集会講演要旨集　140回　100-100　2005/08
Publisher: (公社)日本獣医学会
ISSN： 1347-8621

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Books and Other Publications 12

仙台経済界 2025年1-2月号

2025/01
仙台経済界臨時増刊号「2024せんだい自遊人クラブ」

仙台経済界　2024/07
「JVPA DIGEST」原虫の感染・増殖メカニズムと抗原虫薬の開発について

加藤健太郎

公益社団法人日本動物用医薬品協会　2020/06

ISBN: 4820494171
Bio industry = バイオインダストリー : 工業化をめざすバイオ専門情報誌

加藤健太郎

シーエムシー　2019/06
医学のあゆみ C型肝炎SVR後の肝発癌 2016年 259巻4号 [雑誌]

村越ふみ、加藤健太郎

医歯薬出版　2016/10/21
化学と生物 2015年 06 月号 [雑誌]

加藤健太郎、レクエンコ・フランセス

国際文献社　2015/05/28
寄生虫学研究 2014年版―材料と方法

高宮信三郎

三恵社　2014/12

ISBN: 4864873534
寄生虫学研究 2013年版―材料と方法

浅川満彦

三恵社　2013/12

ISBN: 486487137X
寄生虫学研究 2012年版―材料と方法

丸山治彦, 宇賀昭二

三恵社　2012/06/01

ISBN: 4883619982
獣医微生物学実験マニュアル

本多英一, 本多英一, 原澤亮, 原澤亮

チクサン出版社　2009/09/01

ISBN: 4885006643
動物微生物学

明石博臣, 原澤亮, 本多英一, 木内明男

朝倉書店　2008/04/01

ISBN: 4254460287
新獣医学辞典

新獣医学辞典編集委員会

チクサン出版社　2008/02/01

ISBN: 4885006546

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Industrial Property Rights 14

マラリア原虫の赤血球への侵入阻害剤

加藤健太郎, 伴戸寛徳, オラワレジェジェ

Property Type: Patent
防除用製剤、並びに土壌処理方法

加藤健太郎、大川和久、升水紀郎

Property Type: Patent
抗トキソプラズマ原虫剤

加藤健太郎, アデエミオルヨミ

Property Type: Patent
抗原虫作用を持つヒストン脱アセチル化酵素阻害剤

加藤健太郎, 村越ふみ

Property Type: Patent
抗原虫作用を持つ生薬由来化合物と生薬エキス

加藤健太郎, 村田優穂, 杉達紀, 野中基弘

Property Type: Patent
抗トキソプラズマ剤及びこれを含む医薬

加藤健太郎, アデエミオルヨミ

Property Type: Patent
抗トキソプラズマ剤

加藤健太郎, 村田優穂, 杉達紀

Property Type: Patent
抗トキソプラズマ剤

加藤健太郎, 村田優穂, 杉達紀

Property Type: Patent
抗原虫薬

加藤健太郎, アラーテルカウィ, 高野量

Property Type: Patent
抗原虫薬のスクリーニング方法及び組換えトキソプラズマ株

加藤健太郎, 杉達紀, 正谷達謄

Property Type: Patent
原虫プロテインキナーゼの即時的機能抑制が可能なトキソプラズマ原虫株

加藤健太郎, 明石博臣, 杉達紀

Property Type: Patent
熱帯熱マラリア原虫の雄性ガメトサイトマーカー

加藤健太郎

Property Type: Patent
動物用ワクチン

加藤健太郎, 明石博臣, 遠矢幸伸, 有井潤

Property Type: Patent
抗クリプトスポリジウム薬及びクリプトスポリジウム症の予防又は治療方法

加藤健太郎, ビンカビル, モハメドハジャズ

Property Type: Patent

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Research Projects 23

新しいクリプトスポリジウム症対策を目指した原虫-宿主細胞間インタラクトーム解析

加藤健太郎

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2024/04/01 - 2027/03/31
Detailed mechanism of trafficking proteins involved in the virulence of Plasmodium falciparum

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows

Category: Grant-in-Aid for JSPS Fellows

Institution: Tohoku University

2022/07/27 - 2024/03/31
Development and characterization of novel drugs against cryptosporidiosis with outbreak of diarrhea

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows

Category: Grant-in-Aid for JSPS Fellows

Institution: Tohoku University

2021/07/28 - 2024/03/31
疫学と数理モデルを融合したコウモリ由来感染症のリスク分析

大松勉, 久和茂, 加藤健太郎, 宇根ユミ, 藤井ひかる, 松山亮太

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 国際共同研究加速基金(国際共同研究強化(B))

Institution: 東京農工大学

2019/10/07 - 2024/03/31

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現在世界各地で流行するCOVID-19の原因ウイルスであるSARS-CoV-2や西アフリカで流行するエボラウイルス、東南アジアで発生したニパウイルスなど、世界的に問題となっているウイルスの自然宿主としてコウモリは知られている。今後も、コウモリから新たなウイルス感染症が発生する可能性は否定できない。また、コウモリを介した細菌や寄生虫の拡散リスクについてはほとんど明らかになっていない。本研究では、フィリピン国内に生息する翼手目（コウモリ）を対象としたウイルス・細菌・寄生虫の網羅的な疫学調査とフィリピンに生息するコウモリの生態情報を融合し、コウモリ由来感染症のリスクを明らかにすることを目的としている。令和元年度は、フィリピン側共同研究者と複数回メールでの打ち合わせを行った上で、現地において日本側共同研究者とフィリピン側共同研究者との会合を行い、全体の研究計画を確認すると共に疫学調査地に関する情報交換を行い、日本側研究者が参加する現地における大規模調査とは別にフィリピン側共同研究者が継続して実施している生態調査時にも病原体保有調査用のサンプリングを実施することとなった。また、生態情報に関して収集する情報の項目について情報交換を行い、データベース構築に関する作業工程を確認した。現地疫学調査としては、定点調査候補地としてルソン島中部を選定し、コウモリの捕獲調査を行いオオコウモリ、ココウモリ含め、約120頭のコウモリを捕獲した。今後、コロナウイルスやエボラウイルス等のウイルス、Campylobacter jejuni等の細菌、アイメリア等の寄生虫の保有状況についてPCRやELISA等の手法を用いて解析する。また、次回調査地としてパラワン島を選定し、資材等の準備を進める予定である。。
Analyses on membrane trafficking mechanism related to severe malaria

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2020/04/01 - 2023/03/31
原虫LDHをターゲットとしたズーノーシスの感染制御系の確立

加藤健太郎, LI KUN

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 特別研究員奨励費

Institution: 東北大学

2020/04/24 - 2022/03/31
Analyses on innate immune response for vector-borne virus.

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for JSPS Fellows

Institution: Tohoku University

2019/07/24 - 2022/03/31
Establishment of amino acid-capped metal nanoparticle for enhancing anti-protozoan activity

KATO Kentaro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Tohoku University

2019/06/28 - 2022/03/31

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Toxoplasma gondii causes toxoplasmosis, a common infection against which better drugs are needed. Recently, we showed that inorganic nanoparticles have anti-Toxoplasma activity. Here, we sought to enhance the anti-parasitic efficacy and host biocompatibility of these nanoparticles by modifying their surface with amino acids. Amino acid-capped nanoparticles (amino-NPs) were synthesized, purified, and then screened for anti-Toxoplasma activity in in vitro infection models. The amino-NPs showed enhanced anti-parasitic selectivity as well as improved host biocompatibility. Oxidative stress, modulation of host HIF-1α, and activation of the kynurenine pathway contributed to the anti-parasitic action of the amino-NPs.
Analyses on virus symbiosis mechanism inside and outside of protozoa.

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Institution: Tohoku University

2019/04/01 - 2021/03/31
Comprehensive analyses in components of Toxoplasma dormant parasites.

KATO Kentaro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

2017/04/01 - 2020/03/31

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This research focuses on molecular mechanism of Toxoplasma latent infection, which has not been studied yet. By the characterization of cyst, a dormant parasite, which is a source of infection, we performed to select parasite factors enough which can be targeted for anti-parasite drugs. At first, we detected the parasite factors constituting cyst wall comprehensively in the dormant Toxoplasma-infected cells. Moreover, we characterized the detected parasite factors in the latent infection.
Collection of epidemiological data on bat-derived infectious disease risk derived from wing hand eyes for correspondence strategic construction

Kyuwa Shigeru

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: The University of Tokyo

2017/04/01 - 2020/03/31

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The purpose of this study is to collect bats in the Philippines and Taiwan for the epidemiological investigations on bacteria, viruses and protozoans which bats have, and to build up the basic data for making the correspondence strategy for the bat-derived infectious diseases in Japan possibly by climate change in the future. As we had not experienced bat collection in Taiwan, we performed it for preliminary investigation in 2017. It turned out that both species and the number of bats which we could collect were limited in Taiwan, and we gave it up. We collected approximately 170 bats in the Philippines in January and June, 2019. Unfortunately, we could not complete the examination within the research period, but would like to report the results near future.
Ecology between livestock and infectious microbes to maintain food circulation

KATO Kentaro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Obihiro University of Agriculture and Veterinary Medicine

2016/07/19 - 2020/03/31

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In the food circulation, the production of food derived from livestock is decreased significantly by protozoan diseases with viral or bacterial ones. This study was focused on the protozoan diseases which inhibit food production of livestock meat in the food circulation. We analyzed molecular biology and pathogenic epidemiology in the field in Japan and foreign countries. The animal infectious experiments, detection system of parasite infection countries by using the parasite symbiotic viruses have been performed. We have studied lives of livestock, wild animals, and microbes, and agricultural ecology as a substance circulation of environment essential for them for a long time.
Exploitation of nanotechnology for novel anti-protozoa strategy

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for JSPS Fellows

Institution: Obihiro University of Agriculture and Veterinary Medicine

2015/10/09 - 2018/03/31
Elucidation of mechanisms by which host immune cells kill protozoan parasites and development of antiparasitic peptides

Terkawi Alaa, Kato Kentaro, Takano Ryo

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (B)

Institution: Hokkaido University

2015/04/01 - 2017/03/31

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Understanding the molecular defense mechanism of phagocytes and identifying their effector molecules against malarial parasites may provide important clues for the discovery of new therapies. Macrophages and neutrophils are professional phagocytes that play key role in the innate immune response against infection through their ability to rapidly recognize and kill microorganisms. An attempt was made to identify the host effector molecules derived from macrophages and neutrophils that kill malaria parasites using DNA microarray technology. Results suggested that DEFB130 from macrophages and CTSL from neutrophils are involved in the clearance mechanism of malarial parasites. The data obtained from this project broaden our knowledge on the immunological response of macrophages and neutrophils to malaria parasites and shed light on a new target for therapeutic intervention.
原生動物の宿主細胞侵入マシナリーの作動原理の解明と構造解析

加藤健太郎

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 新学術領域研究(研究領域提案型)

Institution: 帯広畜産大学

2015/04/01 - 2017/03/31

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研究代表者の研究グループでは、現在までこの“宿主細胞侵入マシナリー”の作動原理を明らかとするため、Glideosome及びMoving junction複合体の構成蛋白質と内外の蛋白質との相互作用やその駆動の端緒となるシグナル伝達系について解析を行ってきた。本研究の目的は、これまで研究代表者らが行ってきた原生動物についての研究成果を発展させ、原生動物の宿主細胞侵入マシナリー、つまりGlideosomeとMoving junction (MJ)複合体を中心とした運動装置がいかにして作動するかという命題に焦点をしぼり、現在わかっていないモーター蛋白質や作動シグナル伝達系も含めて詳細に解析を行うことにある。本年度は、MJ複合体の構成蛋白質であるRONファミリーと宿主細胞の骨格分子であるチューブリン、アクチン等との相互作用の解析を行った。
Identification of trigger factor for the differentiation of Toxoplasma bradyzoite

KATO Kentaro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Obihiro University of Agriculture and Veterinary Medicine

2013/04/01 - 2016/03/31

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We succeeded in the detection of parasite factor associated with dormant differentiation by the whole genome sequence analyses. We established the experimental system that Toxoplasma cysts can be formed in mice brain. The effects of drugs were analyzed using this system. The recombinant parasite was manufactured which have the luminescent gene downstream of the promoter of gene associated with dormant differentiation. The drug screening system which selects the inhibitors of dormant differentiation by the luminescent activity was established.
Roles of cabohydrates in protozoan infection

KATO Kentaro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (A)

Institution: Obihiro University of Agriculture and Veterinary Medicine

2012/04/01 - 2016/03/31

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We got the results of roles of cabohydrates in protozoan infection as described below. The host factors bound with protozoan secreted proteins have been detected. The polysaccharides have been sulfated to manufacture materials effective in the inhibition of infection. Using these sulfated polysaccharides, we analyzed the effects on invasion inhibition and growth inhibition. We have detected the protozoan proteins actually bound with the sulfated polysaccharides which can inhibit the protozoan growth. Therefore, we succeeded in analyzing roles of cabohydrates in protozoan infection.
Controlling mechanism of host by endosymbiont organelle derived from plant

NAGAMUNE KISABURO, NISHIKAWA Yoshihumi, KATO Kentaro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Institution: National Institute of Infectious Diseases

2011/04/01 - 2016/03/31

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I found that apicomplexan parasites, such as Toxoplasma and Plasmodium have ability to produce some plant hormones. Especially, Plasmodium had high concentration of salicylic acid and we investigated the role in malarial parasites. I also found that an inhibitor of biosynthesis of abscisic acid inhibited the proliferation of not only Toxoplasma, but also relative parasite, Neospora. Further, I found a chemical which inhibited the growth of tissue cyst of Toxoplasma.
オルガネラ分泌蛋白質による原虫の寄生・共生成立の分子基盤

加藤健太郎

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 新学術領域研究(研究領域提案型)

Institution: 帯広畜産大学

2012/04/01 - 2015/03/31

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原虫は寄生性で特に病原性を持つ単細胞真核生物であり、世界三大感染症の一つであるマラリアは言うまでもなく、人獣共通感染症であるトキソプラズマ、クリプトスポリジウム等の国内外で重篤な被害を出している感染症の病原微生物が数多く属している。原虫は原生生物界に属し、そのゲノムは進化的には渦鞭毛藻類に近縁であることから、植物型の生理、代謝システム、オルガネラ及びその痕跡を有している。元来共生に由来すると考えられるオルガネラとして、ミトコンドリアや葉緑体の痕跡と推測されるapicoplast等が保存されている。一方で内部共生非依存的に独自の進化を遂げたオルガネラとして、microneme, rhoptry, dense granule等が挙げられ、ここから分泌される蛋白質が宿主細胞への寄生・共生の成立に大きく関わっている。apical（吻側）に上に挙げた特徴的なオルガネラを持つことから、これらの原虫はApicomplexaと呼ばれ、上に挙げた原虫を始め、医学・獣医学領域で問題となっている多くの原虫が属する。このように、Apicomplexaは独自に進化したオルガネラを内包し、さらに宿主細胞へと寄生することから、3重のマトリョーシカ構造を形成していることになる。本研究の目的は、原虫のオルガネラ分泌蛋白質が原虫の寄生・共生成立にどのような役割を果たしているのかをオミクス解析、可視化技術等を駆使し、その分子基盤を明らかとすることにある。平成２５年度は、原虫が宿主細胞に侵入する際のAMA1-RONs複合体を構成する分子の挙動の解析を行った。具体的にはRON蛋白質を宿主細胞の骨格分子であるチューブリンとの相互作用について解析を行った。
Elucidation of malaria erythrocyte invasion system using stem cell techonology

KATO Kentaro, TSUJI Koichiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

2011 - 2012

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We confirmed the invasion of Plasmodium falciparum using erythrocyte like cells from human umbilical cord blood hematopoietic stem cell and mouse ES cells. The establishment of effective production system of mature erythrocyte from stem cell has been mainly proceeded. Before the treatment of ES cell and iPS cell, the technique that CD34 positive hematopoietic stem cell are isolated from umbilical cord blood and cultured have been mastered. Afterwards, we investigated the effective condition to lead enucleation with arrangement of class, concentration of cytokine, and culture period. Moreover, to produce the differentiated erythrocyte mutant with specific protein deficient, we tried to establish the trancfection system of differentiated erythrocyte using lentivirus vecter.
Establishment of substrates exploration of protozoan enzymes associated with invasion into host cells

KATO Kentaro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (B)

Institution: The University of Tokyo

2009 - 2010

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We have got some results that protozoa enzymes including protein kinases take parts in the important signal transductions for parasites to transfer from a stage to the next stage by analyzing the substrates and functions of enzymes relating with calcium signaling in their complicated life cycles. These results lead to the new anti-protozoan drug developments to block the protozoa-specific life cycle by inhibiting the functions of the important protozoan enzymes in the stage transfer.
Characterization of protozoan protein kinases aiming at the new anti-protozoan strategy

KATO Kentaro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (A)

Institution: The University of Tokyo

2006 - 2008
新しい抗ウイルス戦略を目指したヘルペスウイルス特異的酵素の解析

加藤健太郎

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 特別研究員奨励費

Institution: 東京大学

2003 - 2004

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本研究の目的は、新しい抗ウイルス戦略を目指したヘルペスウイルス特異的酵素の解析にある。次年度以降に向けての研究に対する土台作りの年と位置づけられる今年度にあって、以下に述べるような満足に足る結果が得られたので報告する。まず、本研究を通じて得られた今年度の具体的な研究成果について説明する。今年度の学会誌等への発表として、次の項にも示す通り、英文論文4報(うち、筆頭著者論文1報、共著者論文2報、レヴィー1報)の成果が得られた。かつ、それらの論文はReviews in Medical Virology、Journal of General Virology等インパクトファクターが3以上の医学系ウイルス学の分野においてトップクラスとされる学術雑誌に掲載されており、初年度の成果としては十分満足のいくものであると言える。具体的な研究成果としてヘルペスウイルス特異的酵素に関していくつの知見が得られたが、特にヘルペスウイルスで共通に保存されているプロテインキナーゼが宿主細胞のプロテインキナーゼの機能を模倣しているという知見は世界的に優れた研究と評価され、医学的、細胞生物学的な見地からインパクトが大きく、今後の研究への広がりが期待される。今年度得られた研究成果、知見は玩具動物、競走馬、畜産動物のヘルペスウイルスのプロテインキナーゼにも当てはまると考えられる普遍的な性質であることから、次年度以降の研究応用の土台として十分なものであり、今後の研究に期待が持たれる。また、学会発表についても今年度は2件行っており、問題はない。

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日本学術振興会「独創の原点」

日本学術振興会　独創の原点

2018/10/24

Type: Internet