Details of the Researcher

PHOTO

Soichi Kojima
Section
Graduate School of Agricultural Science
Job title
Assistant Professor
Degree

  • 博士（農学）（東北大学）

  • 修士（農学）（東北大学）

Researcher ID
S-3907-2017

Research History 5

  • 2007/12 - Present
    Tohoku University　Graduate School of Agricultural Science Division of Life Science　Assistant Professor

  • 2007/12 - 2010/10
    RIKEN　Plant Science Center　Guest Scientist

  • 2007/04 - 2007/11
    RIKEN　Plant Science Center　Research Scienctist

  • 2002/10 - 2007/03
    Universität Hohenheim　Institute für Pflanzenernährung　Wissenschaftlicher Mitarbeiter

  • 2001/04 - 2003/03
    Japan Society for the Promotion of Science　Research Fellowship for Young Scientist

Committee Memberships 5

  • 植物の栄養研究会　幹事

    2023/09 - Present

  • Frontiers in Plant Science　Associate Editor

    2021/04 - Present

  • トランスポーター研究会　幹事

    2008/04 - Present

  • Frontiers in Plant Science　Review Editor

    2010/07 - 2021/04

  • 植物科学最先端研究拠点ネットワーク　支援担当

    2012/04 - 2017/03

Professional Memberships 6

  • 植物の栄養研究会

  • トランスポーター研究会

  • 日本農芸化学会

  • Japanese Society for Plant Cell and Molecular Biology

  • Japanese Society of Soil Science and Plant Nutrition

  • The Japanese Society of Plant Physiologists

︎Show all ︎Show first 5

Research Interests 4

  • Transport

  • Metabolism

  • Nitrogen

  • Plant

Research Areas 1

  • Life sciences / Plant nutrition, soil science /

Awards 6

  1. Winners of the reviewer award 2021

    2022/03　Physiologia Plantarum　Award for review of articles requested by Physiologia Plantarum in 2020 and 2021

  2. The JSPCMB Excellent Paper Award

    2018/08　Japanese Society for Plant Cell and Molecular Biology　The chimeric repressor for the GATA4 transcription factor improves tolerance to nitrogen deficiency in Arabidopsis.

  3. 日本土壌肥料学会2011年度筑波大会ポスター賞

    2011/08　日本土壌肥料学会

  4. 日本土壌肥料学会2010年度北海道大会ポスター賞

    2010/09　日本土壌肥料学会

  5. Outstanding Poster Award in the 1st International Symposium on the Nitrogen Nutrition of Plants

    2010/07/30　Nitrogen2010 organizing comittee

  6. Best poster award in the 15th congress in the Federation of European Societies in Plant Biology

    2006/07　Federation of European Societies in Plant Biology

Show all ︎Show 5

Papers 63

  1. Redefining awn development in rice through the breeding history of Japanese awn reduction International-journal Peer-reviewed

    Mao Suganami, Hideki Yoshida, Shinya Yoshida, Mayuko Kawamura, Eriko Koketsu, Makoto Matsuoka, Soichi Kojima

    Frontiers in Plant Science　15　2024/05/16

    Publisher: Frontiers Media SA

    DOI： 10.3389/fpls.2024.1370956 　

    eISSN： 1664-462X

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    The study challenges the conventional understanding of awn loss as a domestication syndrome, showing instead that many awned varieties continued to be widely grown in Japan until the early twentieth century and that selection for awn reduction was active at that time, demonstrating that awn loss is not a domestication syndrome but “a trait that emerged during crop improvement”. Although selection for awnless mutants was carried out independently using different types of awned cultivars in the early twentieth century in Japan, awn loss was caused by the mutation in OsEPFL1. This suggests that a single mutant haplotype of OsEPFL1 was conserved in the genomes of different cultivars and subsequently selected within each line to meet the demand for awnless varieties. The study also conducts phylogenetic analyses of EPFL1 in 48 grass plants, revealing its unique involvement in awn formation in rice while potentially playing a different role in the domestication of other grass plants. Finally, an attempt is made to isolate an awn-forming gene that has not been identified from the awned rice cultivar “Omachi”, which is still cultivated in Japan. The results presented in this paper provide a new perspective on domestication against the conventional understanding of awn development, shedding light on its potential as a useful organ for breeding to mitigate environmental stress.

  2. Low mutation rate of spontaneous mutants enables detection of causative genes by comparing whole genome sequences International-journal Peer-reviewed

    Mao Suganami, Soichi Kojima, Hideki Yoshida, Masaki Mori, Mayuko Kawamura, Eriko Koketsu, Makoto Matsuoka

    Frontiers in Plant Science　15　2024/04/04

    Publisher: Frontiers Media SA

    DOI： 10.3389/fpls.2024.1366413 　

    eISSN： 1664-462X

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    In the early 1900s, mutation breeding to select varieties with desirable traits using spontaneous mutation was actively conducted around the world, including Japan. In rice, the number of fixed mutations per generation was estimated to be 1.38-2.25. Although this low mutation rate was a major problem for breeding in those days, in the modern era with the development of next-generation sequencing (NGS) technology, it was conversely considered to be an advantage for efficient gene identification. In this paper, we proposed an in silico approach using NGS to compare the whole genome sequence of a spontaneous mutant with that of a closely related strain with a nearly identical genome, to find polymorphisms that differ between them, and to identify the causal gene by predicting the functional variation of the gene caused by the polymorphism. Using this approach, we found four causal genes for the dwarf mutation, the round shape grain mutation and the awnless mutation. Three of these genes were the same as those previously reported, but one was a novel gene involved in awn formation. The novel gene was isolated from Bozu-Aikoku, a mutant of Aikoku with the awnless trait, in which nine polymorphisms were predicted to alter gene function by their whole-genome comparison. Based on the information on gene function and tissue-specific expression patterns of these candidate genes, Os03g0115700/LOC_Os03g02460, annotated as a short-chain dehydrogenase/reductase SDR family protein, is most likely to be involved in the awnless mutation. Indeed, complementation tests by transformation showed that it is involved in awn formation. Thus, this method is an effective way to accelerate genome breeding of various crop species by enabling the identification of useful genes that can be used for crop breeding with minimal effort for NGS analysis.

  3. Coregulation of glutamine synthetase1;2 (GLN1;2) and NADH-dependent glutamate synthase (GLT1) gene expression in Arabidopsis roots in response to ammonium supply International-journal International-coauthorship Peer-reviewed

    Soichi Kojima, Haruka Minagawa, Chika Yoshida, Eri Inoue, Hideki Takahashi, Keiki Ishiyama

    Frontiers in Plant Science　14　2023/02/07

    DOI： 10.3389/FPLS.2023.1127006 　

  4. Effective use of legacy data in a GWAS improves the credibility of QTL detection in rice International-journal Peer-reviewed

    Mao Suganami, Soichi Kojima, Fanmiao Wang, Hideki Yoshida, Kotaro Miura, Yoichi Morinaka, Masao Watanabe, Tsukasa Matsuda, Eiji Yamamoto, Makoto Matsuoka

    Plant Physiology　191　(3)　1561-1573　2023/01/18

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/plphys/kiad018 　

    ISSN： 0032-0889

    eISSN： 1532-2548

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    Genome-wide association studies (GWASs) are used to detect quantitative trait loci (QTLs) using genomic and phenotypic data as inputs. While genomic data are obtained with high throughput and low cost, obtaining phenotypic data requires a large amount of effort and time. In past breeding programs, researchers and breeders have conducted a large number of phenotypic surveys and accumulated results as legacy data. In this study, we conducted a GWAS using phenotypic data of temperate japonica rice (Oryza sativa) varieties from a public database. The GWAS using the legacy data detected several known agriculturally important genes, indicating reliability of the legacy data for GWAS. By comparing the GWAS using legacy data (L-GWAS) and a GWAS using phenotypic data that we measured (M-GWAS), we detected reliable QTLs for agronomically important traits. These results suggest that a L-GWAS is a strong alternative to replicate tests to confirm the reproducibility of QTLs detected by an M-GWAS. In addition, because legacy data have often been accumulated for many traits, it is possible to evaluate the pleiotropic effect of the QTLs identified for the specific trait that we focused on with respect to various other traits. This study demonstrates the effectiveness of using legacy data for GWASs and proposes the use of legacy data to accelerate genomic breeding.

  5. Cytosolic glutamine synthetase GS1;3 is involved in rice grain ripening and germination International-journal Peer-reviewed

    Takayuki Fujita, Marcel P. Beier, Mayumi Tabuchi-Kobayashi, Yoshitaka Hayatsu, Haruka Nakamura, Toshiko Umetsu-Ohashi, Kazuhiro Sasaki, Keiki Ishiyama, Emiko Murozuka, Mikiko Kojima, Hitoshi Sakakibara, Yuki Sawa, Akio Miyao, Toshihiko Hayakawa, Tomoyuki Yamaya, Soichi Kojima

    Frontiers in Plant Science　13　(835835)　835835-835835　2022/01

    Publisher: Frontiers Media

    DOI： 10.3389/fpls.2022.835835 　

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    Ammonium is combined with glutamate to form glutamine. This reaction is catalyzed by glutamine synthetase (GS or GLN). Plants harbor several isoforms of cytosolic GS (GS1). Rice GS1;3 is highly expressed in seeds during grain filling and germination, suggesting a unique role in these processes. This study aimed to investigate the role of GS1;3 for rice growth and yield. Tos17 insertion lines for GS1;3 were isolated, and the nitrogen (N), amino acid, and ammonium contents of GS1;3 mutant grains were compared to wild-type grains. The spatiotemporal expression of GS1;3 and the growth and yield of rice plants were evaluated in hydroponic culture and the paddy field. Additionally, the stable isotope of N was used to trace the foliar N flux during grain filling. Results showed that the loss of GS1;3 retarded seed germination. Seeds of GS1;3 mutants accumulated glutamate but did not show a marked change in the level of phytohormones. The expression of GS1;3 was detected at the beginning of germination, with limited promoter activity in seeds. GS1;3 mutants showed a considerably decreased ripening ratio and decreased N efflux in the 12th leaf blade under N deficient conditions. The β-glucuronidase gene expression under control of the GS1;3 promoter was detected in the vascular tissue and aleurone cell layer of developing grains. These data suggest unique physiological roles of GS1;3 in the early stage of seed germination and grain filling under N deficient conditions in rice.

  6. The function of high‐affinity urea transporters in nitrogen‐deficient conditions International-journal Peer-reviewed

    Marcel P. Beier, Soichi Kojima

    Physiologia Plantarum　2021/04

    DOI： 10.1111/ppl.13303 　

  7. Ammonium assimilation and metabolism in rice International-journal Invited Peer-reviewed

    Soichi Kojima, Keiki Ishiyama, Marcel Beier, Toshihiko Hayakawa

    Progress in Botany　211-231　2020/08

    Publisher: Springer

    DOI： 10.1007/124_2020_40 　

    ISSN： 0340-4773

    eISSN： 2197-8492

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    Rice sustains the world. It is the staple food for most Asian countries. Both high yield and healthy growth of rice are highly dependent on nitrogen fertilizer. Ammonium is the most important nitrogen source for rice growth. Rice is grown in paddy-fields where nitrification is inhibited because of anaerobic conditions. Ammonium transporters (AMT) transport ammonium from the soil into plant cells in roots. Glutamine synthetase (GS) ligates ammonium with glutamate in an ATP dependent manner, thus it is a key enzyme of ammonium assimilation. Glutamate synthase (GOGAT) transfers the amide residue of glutamine to 2-oxoglutarate to yield two molecules of glutamate. Since GS and GOGAT provide substrates to each other, their reaction is coupled. The GS/GOGAT cycle is the major pathway in the ammonium assimilation in plants. The rice genome encodes several isozymes for GS/GOGAT. In this chapter, we describe the physiological functions and “job-sharing” of three isoenzymes of cytosolic GS (GS1;1, GS1;2, and GS1;3) and two NADH-GOGAT (NADH-GOGAT1 and NADH-GOGAT2) enzymes by the use of a reverse genetics approach and a biochemical approach.

  8. The urea transporter DUR3 contributes to rice production under nitrogen-deficient and field conditions Peer-reviewed

    Marcel P. Beier, Takayuki Fujita, Kazuhiro Sasaki, Keiichi Kanno, Miwa Ohashi, Wataru Tamura, Noriyuki Konishi, Masahide Saito, Fumi Imagawa, Keiki Ishiyama, Akio Miyao, Tomoyuki Yamaya, Soichi Kojima

    Physiologia Plantarum　167　(1)　75-89　2019/09

    Publisher: Wiley

    DOI： 10.1111/ppl.12872 　

    ISSN： 0031-9317

    eISSN： 1399-3054

  9. Identification of genomic regions regulating ammonium-dependent inhibition of primary root length in Arabidopsis thaliana International-journal Peer-reviewed

    Kazuhiro Sasaki, Soichi Kojima

    Soil Science and Plant Nutrition　64　(6)　746-751　2018/11/02

    DOI： 10.1080/00380768.2018.1524268 　

    ISSN： 0038-0768

    eISSN： 1747-0765

  10. Tos17 insertion in NADH-dependent glutamate synthase genes leads to an increase in grain protein content in rice Peer-reviewed

    Fumi Imagawa, Haruka Minagawa, Yosuke Nakayama, Keiichi Kanno, Toshihiko Hayakawa, Soichi Kojima

    Journal of Cereal Science　84　38-43　2018/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.jcs.2018.09.008 　

    ISSN： 0733-5210

  11. The possible interaction of ammonium and auxin polar transport on root system architectures in the two ecotypes of Arabidopsis thaliana International-journal Peer-reviewed

    Soichi Kojima

    Soil Science and Plant Nutrition　64　(5)　616-622　2018/09/03

    Publisher: Informa UK Limited

    DOI： 10.1080/00380768.2018.1507603 　

    ISSN： 0038-0768

    eISSN： 1747-0765

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    Plant growth requires mineral nutrients from soil environment. It is important for plant to develop its root to import mineral nutrient from environment. Both inorganic nitrogen source and plant hormone auxin influences the root system architecture (RSA). Previous study indicated that polar auxin transport is partly involved in ammonium-inhibition of primary root in an Arabidopsis ecotype, Columbia 0 (Col-0), however, the effect of auxin in other ecotypes remains unclear. The purpose of this study is to describe and examine the effect of TIBA, an inhibitor for the auxin polar transport, in ammonium supply-dependent changes of root development. Two Arabidopsis ecotypes, Col-0 and Landsberg erecta 2 (Ler-2), were used due to their different response to ammonium supply. The changes of the RSA in response to ammonium supply on vertical agar medium containing three levels of TIBA and four levels of ammonium were determined in two ecotypes. The primary root length of Ler-2 was markedly shortened by increasing ammonium concentration in the medium, while that of Col-0 was relatively insensitive to ammonium. Conversely, the lateral root length of Ler-2 was increased by ammonium supply. Both primary and lateral root of Col-0 were more sensitive to TIBA than those of Ler-2. ANOVA indicated the significant interaction of TIBA and ammonium in Col-0, however, no interaction in Ler-2. These results suggested the genetic diversity in the interactive effect of auxin and ammonium.

  12. Cytosolic Glutamine Synthetase Isozymes Play Redundant Roles in Ammonium Assimilation Under Low-Ammonium Conditions in Roots of Arabidopsis thaliana International-journal Peer-reviewed

    Noriyuki Konishi, Masahide Saito, Fumi Imagawa, Keiich Kanno, Tomoyuki Yamaya, Soichi Kojima

    PLANT AND CELL PHYSIOLOGY　59　(3)　601-613　2018/03

    DOI： 10.1093/pcp/pcy014 　

    ISSN： 0032-0781

    eISSN： 1471-9053

  13. Ammonium uptake capacity and response of cytosolic glutamine synthetase 1;2 to ammonium supply are key factors for the adaptation of ammonium nutrition in Arabidopsis thaliana International-journal Peer-reviewed

    Takanori Yasuda, Noriyuki Konishi, Soichi Kojima

    Soil Science and Plant Nutrition　63　(6)　553-560　2017/10/26

    DOI： 10.1080/00380768.2017.1395292 　

    ISSN： 0038-0768

    eISSN： 1747-0765

  14. Transcriptional repressor IAA17 is involved in nitrogen use by modulating cytosolic glutamine synthetase GLN1;2 in Arabidopsis roots International-journal Peer-reviewed

    Masahide Saito, Noriyuki Konishi, Keiichi Kanno, Tomoyuki Yamaya, Soichi Kojima

    Soil Science and Plant Nutrition　63　(2)　163-170　2017/03/04

    DOI： 10.1080/00380768.2017.1314178 　

    ISSN： 0038-0768

    eISSN： 1747-0765

  15. A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains International-journal Peer-reviewed

    Yui Yabuki, Miwa Ohashi, Fumi Imagawa, Keiki Ishiyama, Marcel Pascal Beier, Noriyuki Konishi, Toshiko Umetsu-Ohashi, Toshihiko Hayakawa, Tomoyuki Yamaya, Soichi Kojima

    Rice　10　(1)　2017/01/25

    DOI： 10.1186/s12284-017-0143-8 　

    ISSN： 1939-8425

    eISSN： 1939-8433

  16. Contributions of two cytosolic glutamine synthetase isozymes to ammonium assimilation in Arabidopsis roots International-journal International-coauthorship Peer-reviewed

    Noriyuki Konishi, Keiki Ishiyama, Marcel Pascal Beier, Eri Inoue, Keiichi Kanno, Tomoyuki Yamaya, Hideki Takahashi, Soichi Kojima

    Journal of Experimental Botany　erw454-erw454　2016/12/21

    DOI： 10.1093/jxb/erw454 　

    ISSN： 0022-0957

    eISSN： 1460-2431

  17. Urea retranslocation from senescing Arabidopsis leaves is promoted by DUR3-mediated urea retrieval from leaf apoplast Peer-reviewed

    Anne Bohner, Soichi Kojima, Mohammad Hajirezaei, Michael Melzer, Nicolaus von Wiren

    PLANT JOURNAL　81　(3)　377-387　2015/02

    DOI： 10.1111/tpj.12740 　

    ISSN： 0960-7412

    eISSN： 1365-313X

  18. NADH-dependent glutamate synthase plays a crucial role in assimilating ammonium in the Arabidopsis root Peer-reviewed

    Noriyuki Konishi, Keiki Ishiyama, Kaya Matsuoka, Ikumi Maru, Toshihiko Hayakawa, Tomoyuki Yamaya, Soichi Kojima

    PHYSIOLOGIA PLANTARUM　152　(1)　138-151　2014/09

    DOI： 10.1111/ppl.12177 　

    ISSN： 0031-9317

    eISSN： 1399-3054

  19. Cytosolic glutamine synthetase1;2 is responsible for the primary assimilation of ammonium in rice roots Peer-reviewed

    Kazuhiro Funayama, Soichi Kojima, Mayumi Tabuchi-Kobayashi, Yuki Sawa, Yosuke Nakayama, Toshihiko Hayakawa, Tomoyuki Yamaya

    Plant and Cell Physiology　54　(6)　934-943　2013/06

    DOI： 10.1093/pcp/pct046 　

    ISSN： 0032-0781 1471-9053

  20. Disruption of a novel NADH-glutamate synthase2 gene caused marked reduction in spikelet number of rice Peer-reviewed

    Wataru Tamura, Soichi Kojima, Ayako Toyokawa, Hideo Watanabe, Mayumi Tabuchi-Kobayashi, Toshihiko Hayakawa, Tomoyuki Yamaya

    FRONTIERS IN PLANT SCIENCE　2　(57)　1-9　2011

    DOI： 10.3389/fpls.2011.00057 　

    ISSN： 1664-462X

  21. Ammonium Triggers Lateral Root Branching in Arabidopsis in an AMMONIUM TRANSPORTER1;3-Dependent Manner Peer-reviewed

    Joni E. Lima, Soichi Kojima, Hideki Takahashi, Nicolaus von Wiren

    PLANT CELL　22　(11)　3621-3633　2010/11

    DOI： 10.1105/tpc.110.076216 　

    ISSN： 1040-4651

  22. Reverse genetics approach to characterize a function of NADH-glutamate synthase1 in rice plants Invited Peer-reviewed

    Wataru Tamura, Yusuke Hidaka, Mayumi Tabuchi, Soichi Kojima, Toshihiko Hayakawa, Tadashi Sato, Mitsuhiro Obara, Mikiko Kojima, Hitoshi Sakakibara, Tomoyuki Yamaya

    AMINO ACIDS　39　(4)　1003-1012　2010/10

    DOI： 10.1007/s00726-010-0531-5 　

    ISSN： 0939-4451

  23. AtDUR3 represents the major transporter for high-affinity urea transport across the plasma membrane of nitrogen-deficient Arabidopsis roots Peer-reviewed

    Soichi Kojima, Anne Bohner, Brigitte Gassert, Lixing Yuan, Nicolaus von Wiren

    PLANT JOURNAL　52　(1)　30-40　2007/10

    DOI： 10.1111/j.1365-313X.2007.03223.x 　

    ISSN： 0960-7412

  24. The organization of high-affinity ammonium uptake in Arabidopsis roots depends on the spatial arrangement and biochemical properties of AMT1-type transporters Peer-reviewed

    Lixing Yuan, Dominique Loque, Soichi Kojima, Sabine Rauch, Keiki Ishiyama, Eri Inoue, Hideki Takahashi, Nicolaus von Wiren

    PLANT CELL　19　(8)　2636-2652　2007/08

    DOI： 10.1105/tpc.107.052134 　

    ISSN： 1040-4651

  25. Additive contribution of AMT1;1 and AMT1;3 to high-affinity ammonium uptake across the plasma membrane of nitrogen-deficient Arabidopsis roots Peer-reviewed

    Dominique Loque, Lixing Yuan, Soichi Kojima, Alain Gojon, Judith Wirth, Sonia Gazzarrini, Keiki Ishiyama, Hideki Takahashi, Nicolaus von Wiren

    PLANT JOURNAL　48　(4)　522-534　2006/11

    DOI： 10.1111/j.1365-313X.2006.02887.x 　

    ISSN： 0960-7412

  26. Analysis of a promoter for the NADH-glutamate synthase gene in rice (Oryza sativa): cell type-specific expression in developing organs of transgenic rice plants Peer-reviewed

    S Kojima, M Kimura, Y Nozaki, T Yamaya

    AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY　27　(8-9)　787-793　2000

    ISSN： 0310-7841

  27. Phosphorus fertility regulates microbial carbon use efficiency and SOM decomposition in non-allophanic Andosols

    Wako Koizumi, Timothy J Clough, Soichi Kojima, Tomoyuki Makino, Soh Sugihara, Ryosuke Tajima, Yoshitaka Uchida, Toru Hamamoto

    2025/04/29

    DOI： 10.1101/2025.04.29.651165 　

  28. Proposal of an in silico approach to identify the causative gene of natural spontaneous mutants by whole genome sequence comparison.

    Suganami Mao, Kojima Soichi, Yoshida Hideki, Matsuoka Makoto

    Abstracts of Meeting of the CSSJ　258　119-119　2024/09/26

    Publisher: CROP SCIENCE SOCIETY OF JAPAN

    DOI： 10.14829/jcsproc.258.0_119 　

  29. Establishment of Population Consisted of Japanese Soybeans for GWAS

    Mao SUGANAMI, Hidekazu TAKAHASHI, Naoto NIHEI, Hideki YOSHIDA, Soichi KOJIMA, Masao WATANABE, Tsukasa MATSUDA, Makoto MATSUOKA

    Tohoku Journal of Crop Science　(66)　41-42　2023/12

    DOI： 10.20725/ntjcs.66.0_41 　

  30. 4-2-4 複数形質のGWASの比較によるQTLの特異性・多面性の評価（4-2 植物の微量栄養素 2023年度愛媛大会）

    菅波 眞央, 高橋 秀和, 二瓶 直登, 吉田 英樹, 小島 創一, 佐藤 郁恵, 渡辺 正夫, 松田 幹, 松岡 信

    日本土壌肥料学会講演要旨集　69　56-56　2023/09/04

    Publisher: 一般社団法人 日本土壌肥料学会

    DOI： 10.20710/dohikouen.69.0_56_1 　

    ISSN： 0288-5840

    eISSN： 2424-0575

  31. Establishment of populations mainly consisted of Japanese soybeans for GWAS

    Suganami Mao, Takahashi Hidekazu, Yoshida Hideki, Nihei Naoto, Ishikawa Daitaro, Maki Masayasu, Sato Ikue, Yoshida Shinya, Kojima Soichi, Watanabe Masao, Matsuda Tsukasa, Matsuoka Makoto

    Abstracts of Meeting of the CSSJ　254　64-64　2022/09/18

    Publisher: CROP SCIENCE SOCIETY OF JAPAN

    DOI： 10.14829/jcsproc.254.0_64 　

  32. GWAS Using Legacy data Accelerates Genomic Breeding

    Suganami Mao, Kojima Soichi, Yoshida Hideki, Wang Fanmiao, Morinaka Yoichi, Watanabe Masao, Matsuda Tsukasa, Matsuoka Makoto

    Abstracts of Meeting of the CSSJ　253　88-88　2022/03/25

    Publisher: CROP SCIENCE SOCIETY OF JAPAN

    DOI： 10.14829/jcsproc.253.0_88 　

  33. Rice amino acid transporter-like 6 (OsATL6) is involved in amino acid homeostasis by modulating the vacuolar storage of glutamine in roots International-journal Peer-reviewed

    Ogasawara, Saori, Ezaki, Masataka, Ishida, Ryusuke, Sueyoshi, Kuni, Saito, Shunya, Hiradate, Yuki, Kudo, Toru, Obara, Mitsuhiro, Kojima, Soichi, Uozumi, Nobuyuki, Tanemura, Kentaro, Hayakawa, Toshihiko

    Plant Journal　107　(6)　1616-1630　2021/06/27

    DOI： 10.1111/tpj.15403 　

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    Glutamine is a product of ammonium (NH4 + ) assimilation catalyzed by glutamine synthetase (GS) and glutamate synthase (GOGAT). The growth of NH4 + -preferring paddy rice (Oryza sativa L.) depends on root NH4 + assimilation and the subsequent root-to-shoot allocation of glutamine; however, little is known about the mechanism of glutamine storage in roots. Here, using transcriptome and reverse genetics analyses, we show that the rice amino acid transporter-like 6 (OsATL6) protein exports glutamine to the root vacuoles under NH4 + -replete conditions. OsATL6 was expressed, along with OsGS1;2 and OsNADH-GOGAT1, in wild-type (WT) roots fed with sufficient NH4 Cl, and was induced by glutamine treatment. We generated two independent Tos17 retrotransposon insertion mutants showing reduced OsATL6 expression to determine the function of OsATL6. Compared with segregants lacking the Tos17 insertion, the OsATL6 knock-down mutant seedlings exhibited lower root glutamine content but higher glutamine concentration in the xylem sap and greater shoot growth under NH4 + -replete conditions. The transient expression of monomeric red fluorescent protein-fused OsATL6 in onion epidermal cells confirmed the tonoplast localization of OsATL6. When OsATL6 was expressed in Xenopus laevis oocytes, glutamine efflux from the cell into the acidic bath solution increased. Under sufficient NH4 + supply, OsATL6 transiently accumulated in sclerenchyma and pericycle cells, which are located adjacent to the Casparian strip, thus obstructing the apoplastic solute path, and in vascular parenchyma cells of WT roots before the peak accumulation of GS1;2 and NADH-GOGAT1 occurred. These findings suggest that OsATL6 temporarily stores excess glutamine, produced by NH4 + assimilation, in root vacuoles before it can be translocated to the shoot.

  34. Cytosolic GLUTAMINE SYNTHETASE1;1 Modulates Metabolism and Chloroplast Development in Roots. International-journal Peer-reviewed

    Miyako Kusano, Atsushi Fukushima, Mayumi Tabuchi-Kobayashi, Kazuhiro Funayama, Soichi Kojima, Kyonoshin Maruyama, Yoshiharu Y Yamamoto, Tomoko Nishizawa, Makoto Kobayashi, Mayumi Wakazaki, Mayuko Sato, Kiminori Toyooka, Kumiko Osanai-Kondo, Yoshinori Utsumi, Motoaki Seki, Chihaya Fukai, Kazuki Saito, Tomoyuki Yamaya

    Plant physiology　182　(4)　1894-1909　2020/04

    DOI： 10.1104/pp.19.01118 　

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    Nitrogen (N) is an essential macronutrient, and the final form of endogenous inorganic N is ammonium, which is assimilated by Gln synthetase (GS) into Gln. However, how the multiple isoforms of cytosolic GSs contribute to metabolic systems via the regulation of ammonium assimilation remains unclear. In this study, we compared the effects of two rice (Oryza sativa) cytosolic GSs, namely OsGS1;1 and OsGS1;2, on central metabolism in roots using reverse genetics, metabolomic and transcriptomic profiling, and network analyses. We observed (1) abnormal sugar and organic N accumulation and (2) significant up-regulation of genes associated with photosynthesis and chlorophyll biosynthesis in the roots of Osgs1;1 but not Osgs1;2 knockout mutants. Network analysis of the Osgs1;1 mutant suggested that metabolism of Gln was coordinated with the metabolic modules of sugar metabolism, tricarboxylic acid cycle, and carbon fixation. Transcript profiling of Osgs1;1 mutant roots revealed that expression of the rice sigma-factor (OsSIG) genes in the mutants were transiently upregulated. GOLDEN2-LIKE transcription factor-encoding genes, which are involved in chloroplast biogenesis in rice, could not compensate for the lack of OsSIGs in the Osgs1;1 mutant. Microscopic analysis revealed mature chloroplast development in Osgs1;1 roots but not in the roots of Osgs1;2, Osgs1;2-complemented lines, or the wild type. Thus, organic N assimilated by OsGS1;1 affects a broad range of metabolites and transcripts involved in maintaining metabolic homeostasis and plastid development in rice roots, whereas OsGS1;2 has a more specific role, affecting mainly amino acid homeostasis but not carbon metabolism.

  35. Reduction in sucrose contents by downregulation of fructose-1,6-bisphosphatase 2 causes tiller outgrowth cessation in rice mutants lacking glutamine synthetase1;2. International-journal Peer-reviewed

    Ohashi M, Ishiyama K, Kusano M, Fukushima A, Kojima S, Hayakawa T, Yamaya T

    Rice (New York, N.Y.)　11　(1)　65-65　2018/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1186/s12284-018-0261-y 　

    ISSN： 1939-8425

    eISSN： 1939-8433

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    BACKGROUND: Our previous transcriptomic analysis revealed that downregulation of nitrogen and carbon metabolism in the basal portions of the shoots inhibited cytosolic glutamine synthetase1;2 (GS1;2), which severely reduced rice tiller number. In the present study, we used rice mutants lacking GS1;2 (gs1;2 mutants) to determine the contribution of carbon metabolism to tiller growth. RESULTS: Metabolomic analysis indicated the effects of carbon metabolism disorder such as reductions in the levels of sugar metabolites (e.g., sucrose and glucose 6-phosphate) in the shoot basal portions of the gs1;2 mutant seedlings. Decrease in sucrose caused by the lack of GS1;2 was successfully restored to the wild-type levels by introducing OsGS1;2 cDNA into the mutants. In the basal portions of the shoots, the lack of GS1;2 caused low expression of cytosolic fructose 1,6-bisphosphatase2 (OscFBP2), which is a key cytosolic sucrose synthesis enzyme; it is especially important in the phloem companion cells of the nodal vascular anastomoses. NH4+ supply upregulated OscFBP2 expression in the shoot basal portions of the wild type but not in those of the gs1;2 mutants. Rice mutants lacking cFBPase2 presented with ~ 30% reduction in total cFBPase activity in the basal portions of their shoots. These mutants displayed reductions in sucrose levels of the basal portions of their shoots but not in their leaf blades. They also had relatively lower tiller numbers at the early growth stage. CONCLUSIONS: Metabolomic analysis revealed that the lack of GS1;2 reduced sucrose metabolism in the basal portions of the shoots. Our results indicated that sucrose reduction was caused by the downregulation of OscFBP2 expression in the basal portions of the gs1;2 mutant shoots. The reduction in sucrose content caused by the lack of cFBPase2 resulted in lower tiller number at the early growth stage. Therefore, adequate sucrose supply via cFBPase2 may be necessary for tiller growth in the basal portions of rice shoots.

  36. Outgrowth of Rice Tillers Requires Availability of Glutamine in the Basal Portions of Shoots International-journal Peer-reviewed

    Miwa Ohashi, Keiki Ishiyama, Soichi Kojima, Noriyuki Konishi, Kazuhiro Sasaki, Mitsue Miyao, Toshihiko Hayakawa, Tomoyuki Yamaya

    Rice　11　(1)　2018/12/01

    Publisher: Springer

    DOI： 10.1186/s12284-018-0225-2 　

    ISSN： 1939-8425

    eISSN： 1939-8433

  37. Lack of ACTPK1, an STY kinase, enhances ammonium uptake and use, and promotes growth of rice seedlings under sufficient external ammonium Peer-reviewed

    Marcel P. Beier, Mitsuhiro Obara, Akiko Taniai, Yuki Sawa, Jin Ishizawa, Haruki Yoshida, Narumi Tomita, Tsuyoshi Yamanaka, Yawara Ishizuka, Syuko Kudo, Akira Yoshinari, Shiho Takeuchi, Soichi Kojima, Tomoyuki Yamaya, Toshihiko Hayakawa

    Plant Journal　93　(6)　992-1006　2018/03/01

    Publisher: Blackwell Publishing Ltd

    DOI： 10.1111/tpj.13824 　

    ISSN： 1365-313X 0960-7412

  38. The chimeric repressor for the GATA4 transcription factor improves tolerance to nitrogen deficiency in Arabidopsis Peer-reviewed

    Ji Min Shin, KwiMi Chung, Shingo Sakamoto, Soichi Kojima, Chuan-Ming Yeh, Miho Ikeda, Nobutaka Mitsuda, Masaru Ohme-Takagi

    PLANT BIOTECHNOLOGY　34　(3)　151-158　2017/09

    DOI： 10.5511/plantbiotechnology.17.0727a 　

    ISSN： 1342-4580

  39. Lack of Cytosolic Glutamine Synthetase1;2 Activity Reduces Nitrogen-Dependent Biosynthesis of Cytokinin Required for Axillary Bud Outgrowth in Rice Seedlings Peer-reviewed

    Miwa Ohashi, Keiki Ishiyama, Soichi Kojima, Mikiko Kojima, Hitoshi Sakakibara, Tomoyuki Yamaya, Toshihiko Hayakawa

    PLANT AND CELL PHYSIOLOGY　58　(4)　679-690　2017/04

    DOI： 10.1093/pcp/pcx022 　

    ISSN： 0032-0781

    eISSN： 1471-9053

  40. Growth Properties and Biomass Production in the Hybrid C-4 Crop Sorghum bicolor Peer-reviewed

    Youshi Tazoe, Takashi Sazuka, Miki Yamaguchi, Chieko Saito, Masahiro Ikeuchi, Keiichi Kanno, Soichi Kojima, Ko Hirano, Hideki Kitano, Shigemitsu Kasuga, Tsuyoshi Endo, Hiroo Fukuda, Amane Makino

    PLANT AND CELL PHYSIOLOGY　57　(5)　944-952　2016/05

    DOI： 10.1093/pcp/pcv158 　

    ISSN： 0032-0781

    eISSN： 1471-9053

  41. Autophagy Supports Biomass Production and Nitrogen Use Efficiency at the Vegetative Stage in Rice Peer-reviewed

    Shinya Wada, Yasukazu Hayashida, Masanori Izumi, Takamitsu Kurusu, Shigeru Hanamata, Keiichi Kanno, Soichi Kojima, Tomoyuki Yamaya, Kazuyuki Kuchitsu, Amane Makino, Hiroyuki Ishida

    PLANT PHYSIOLOGY　168　(1)　60-U721　2015/05

    DOI： 10.1104/pp.15.00242 　

    ISSN： 0032-0889

    eISSN： 1532-2548

  42. Asparagine Synthetase1, but not Asparagine Synthetase2, is Responsible for the Biosynthesis of Asparagine Following the Supply of Ammonium to Rice Roots Peer-reviewed

    Miwa Ohashi, Keiki Ishiyama, Soichi Kojima, Noriyuki Konishi, Kentaro Nakano, Keiichi Kanno, Toshihiko Hayakawa, Tomoyuki Yamaya

    PLANT AND CELL PHYSIOLOGY　56　(4)　769-778　2015/04

    DOI： 10.1093/pcp/pcv005 　

    ISSN： 0032-0781

    eISSN： 1471-9053

  43. Lack of cytosolic glutamine synthetase1;2 in vascular tissues of axillary buds causes severe reduction in their outgrowth and disorder of metabolic balance in rice seedlings Peer-reviewed

    Miwa Ohashi, Keiki Ishiyama, Miyako Kusano, Atsushi Fukushima, Soichi Kojima, Atsushi Hanada, Keiichi Kanno, Toshihiko Hayakawa, Yoshiya Seto, Junko Kyozuka, Shinjiro Yamaguchi, Tomoyuki Yamaya

    PLANT JOURNAL　81　(2)　347-356　2015/01

    DOI： 10.1111/tpj.12731 　

    ISSN： 0960-7412

    eISSN： 1365-313X

  44. A candidate factor that interacts with RF2, a restorer of fertility of Lead rice-type cytoplasmic male sterility in rice Peer-reviewed

    Shinya Fujii, Tomohiko Kazama, Yukihiro Ito, Soichi Kojima, Kinya Toriyama

    RICE　7　21　2014/10

    DOI： 10.1186/s12284-014-0021-6 　

    ISSN： 1939-8425

    eISSN： 1939-8433

  45. NADH-dependent glutamate synthase participated in ammonium assimilation in Arabidopsis root Invited Peer-reviewed

    Soichi Kojima, Noriyuki Konishi, Marcel Pascal Beier, Keiki Ishiyama, Ikumi Maru, Toshihiko Hayakawa, Tomoyuki Yamaya

    Plant Signaling and Behavior　9　(8)　e29402　2014/06/04

    DOI： 10.4161/psb.29402 　

    ISSN： 1559-2316

    eISSN： 1559-2324

  46. CLE-CLAVATA1 peptide-receptor signaling module regulates the expansion of plant root systems in a nitrogen-dependent manner Peer-reviewed

    Takao Araya, Mayu Miyamoto, Juliarni Wibowo, Akinori Suzuki, Soichi Kojima, Yumiko N. Tsuchiya, Shinichiro Sawa, Hiroo Fukuda, Nicolaus von Wiren, Hideki Takahashi

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　111　(5)　2029-2034　2014/02

    DOI： 10.1073/pnas.1319953111 　

    ISSN： 0027-8424

  47. Rice novel protein kinase involved in regulation of ammonium uptake into roots under high concentration of external ammonium

    Toshihiko Hayakawa, Mitsuhiro Obara, Akiko Taniai, Yuki Sawa, Jin Ishizawa, Soichi Kojima, Tomoyuki Yamaya

    Abstract book of Nitrogen2013　2013/11/18

  48. Metabolic alterations in free amino acid contents of rice (Oryza sativa l.) seeds during high-humidity storage Peer-reviewed

    K. Sasaki, H. Takahashi, S. Kojima, H. Uchimiya, T. Sato

    Research Journal of Seed Science　6　(1)　16-23　2013

    DOI： 10.3923/rjss.2013.16.23 　

    ISSN： 1819-3552

    eISSN： 2151-6146

  49. Changes of root system by polyamine sypply is dependent on high-affinity urea transporter.

    Keigo Yoshida, Yuki Oiwa, Kaya Tanaka-Matsuoka, Toshihiko Hayakawa, Tomoyuki Yamaya, Nicoiaus von Wirén, Soichi Kojima

    Proceedings of 1st International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2010)　40　2010/07

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    1st International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2010). (Inuyama International Sightseeing Center, Inuyama, Jul. 26-30, 2010)

  50. Interplay between ammonium transport and auxin signaling.

    Soichi Kojima, Yuki Oiwa, Desirée Schüssler, Keigo Yoshida, Kaya Tanaka-Matsuoka, Toshihiko Hayakawa, Tomoyuki Yamaya, Nicoiaus von Wir

    Proceedings of 1st International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2010)　32　2010/07

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    1st International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2010). (Inuyama International Sightseeing Center, Inuyama, Jul. 26-30, 2010)

  51. Reverse genetic approach unravels the physiological functions of NADH-dependent glutamate synthase1 (NADH-GOGAT1) in ammonium assimilation in the root and productivity of rice plant.

    Wataru Tamura, Yusuke Hidaka, Mayumi Tabuchi, Soichi Kojima, MIkiko Kojima, Toshihiko Hayakawa, Hitoshi Sakakibara, Tomoyuki Yamaya

    Proceedings of 1st International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2010)　68　2010/07

    More details Close

    1st International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2010). (Inuyama International Sightseeing Center, Inuyama, Jul. 26-30, 2010)

  52. Cytosolic glutamine symthetase1;2 is one of a key enzymes in ammonium assimilation in root of rice plant.

    Kazuhiro Funayama, Mayumi Tabuchi, Yuki Sawa, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya

    Proceedings of 1st International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2010)　66　2010/07

    More details Close

    1st International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2010). (Inuyama International Sightseeing Center, Inuyama, Jul. 26-30, 2010)

  53. Rice vacuolar membrane-localized amino acid transporter, OsATA1;2, transports glutamine.

    Toshihiko Hayakawa, Ryusuke Ishida, Keisuke Kobayashi, Kuni Sueyoshi, Toru Kudo, Yoko Shiroto, Yoshitsugu Hongo, Soichi Kojima, Tomoyuki Yamaya

    Proceedings of 1st International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2010)　35　2010/07

    More details Close

    1st International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2010). (Inuyama International Sightseeing Center, Inuyama, Jul. 26-30, 2010)

  54. AtAMT1;4, a Pollen-Specific High-Affinity Ammonium Transporter of the Plasma Membrane in Arabidopsis Peer-reviewed

    Lixing Yuan, Lucile Graff, Dominique Loque, Soichi Kojima, Yumiko N. Tsuchiya, Hideki Takahashi, Nicolaus von Wiren

    PLANT AND CELL PHYSIOLOGY　50　(1)　13-25　2009/01

    DOI： 10.1093/pcp/pcn186 　

    ISSN： 0032-0781

  55. Molecular basis on nitrogen utilization in rice. Invited

    Toshihiko Hayakawa, Soichi Kojima, Mayumi Tabuchi, Toru Kudo, Tomoyuki Yamaya

    Tohoku Journal of Agricultural Research　58　(3/4)　113-125　2008/03

    ISSN： 0040-8719

  56. Localization of acid phosphatase activities in the roots of white lupin plants grown under phosphorus-deficient conditions Peer-reviewed

    Jun Wasaki, Soichi Kojima, Hayato Maruyama, Susan Haase, Mitsuru Osaki, Ellen Kandeler

    SOIL SCIENCE AND PLANT NUTRITION　54　(1)　95-102　2008/02

    DOI： 10.1111/j.1747-0765.2007.00207.x 　

    ISSN： 0038-0768

  57. Increased Rubisco content in transgenic rice transformed with the 'sense' rbcS gene Peer-reviewed

    Yuji Suzuki, Maki Ohkubo, Hanako Hatakeyama, Keiko Ohashi, Ryuichi Yoshizawa, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya, Tadahiko Mae, Amane Makino

    PLANT AND CELL PHYSIOLOGY　48　(4)　626-637　2007/04

    DOI： 10.1093/pcp/pcm035 　

    ISSN： 0032-0781

  58. Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene Peer-reviewed

    Yuji Suzuki, Maki Ohkubo, Hanako Hatakeyama, Keiko Ohashi, Ryuichi Yoshizawa, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya, Tadahiko Mae, Amane Makinot

    PLANT AND CELL PHYSIOLOGY　48　(4)　S173-S173　2007

    DOI： 10.1093/pcp/pcm035 　

    ISSN： 0032-0781

  59. Cell type distinct accumulations of mRNA and protein for NADH-dependent glutamate synthase in rice roots in response to the supply of NH4+ Peer-reviewed

    K Ishiyama, S Kojima, H Takahashi, T Hayakawa, T Yamaya

    PLANT PHYSIOLOGY AND BIOCHEMISTRY　41　(6-7)　643-647　2003/06

    DOI： 10.1016/S0981-9428(03)00078-0 　

    ISSN： 0981-9428

  60. Molecular physiology of nitrogen recycling in rice plants Peer-reviewed

    Yamaya T, Kojima S, Obara M, Hayakawa T, Sato T

    Proceedings for the 12th Toyota Conference: Challenge of Plant and Agricultural Sciences to the Crisis of Biosphere on the Earth in the 21st Century　2000

  61. Nucleotide Sequence of a Genomic DNA (Accession No. AB037664) and a cDNA (Accession No.AB037595) Encoding Cytosolic Glutamine Synthetase in Sasanishiki, a Leading Cultivar of Rice (Oryza sativa L) in Northern Japan

    Kojima S, Hanzawa S, Hayakawa T, Hayashi M, Yamaya T

    Plant Physiology　122　1457　2000

  62. Organization and structure of NADH-dependent glutamate synthase gene from rice plants Peer-reviewed

    S Goto, T Akagawa, S Kojima, T Hayakawa, T Yamaya

    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY　1387　(1-2)　298-308　1998/09

    DOI： 10.1016/S0167-4838(98)00142-3 　

    ISSN： 0167-4838

  63. Cell specific expression of NADH-dependent glutamate synthase in rice plants. Peer-reviewed

    K Ishiyama, S Kojima, T Hayakawa, T Yamaya

    PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V　3023-3026　1998

Show all ︎Show first 5

Misc. 43

  1. イネのアンモニウム同化と利用における新奇液胞膜アミノ酸輸送体の機能

    小笠原早織, 江崎正隆, 末吉邦, 小原実広, 小島創一, 魚住信之, 早川俊彦

    日本土壌肥料学会講演要旨集(Web)　66　2020

    ISSN： 2424-0575

  2. イネサイトゾル型グルタミン合成酵素による代謝恒常性と葉緑体発達制御の解明

    草野都, 草野都, 草野都, 圓山恭之進, 福島敦史, 西澤具子, 小林誠, 若崎眞由美, 佐藤繭子, 豊岡公徳, 近藤(小山内)久益子, 内海好規, 関原明, 田渕(小林)眞由美, 舟山和宏, 小島創一, 斉藤和季, 斉藤和季, 山谷知行

    日本植物生理学会年会(Web)　59th　2018

  3. シロイヌナズナの窒素利用におけるオーキシン情報伝達の役割の解析

    齋藤雅英, 小西範幸, 菅野圭一, 山谷知行, 小島創一

    日本土壌肥料学会講演要旨集　63　2017

    ISSN： 0288-5840

  4. Analyzing the role of auxin signal transduction in nitrogen utilization of Arabidopsis thaliana

    SAITO Masahide, KONISHI Noriyuki, KANNO Keiichi, KOJIMA Soichi

    日本植物生理学会年会要旨集　57th　2016

  5. Cytosolic glutamine synthetase 1;2 dependent ammonium assimilation in Arabidopsis

    KONISHI Noriyuki, ISHIYAMA Keiki, KANNO Keiichi, YAMAYA Tomoyuki, KOJIMA Soichi

    日本植物生理学会年会要旨集　57th　2016

  6. シロイヌナズナの根におけるアンモニウムの初期同化

    小西範幸, 小西範幸, 石山敬貴, 菅野圭一, 早川俊彦, 山谷知行, 小島創一

    根の研究　25　(4)　2016

    ISSN： 0919-2182

  7. Seed storage protein in rice mutants for nitrogen metabolism

    32　232-237　2016

    Publisher: 飯島藤十郎記念食品科学振興財団

    ISSN： 2188-0662

  8. コメの生産性を規定するアンモニウム同化系酵素の新規な役割イネにおける窒素代謝に依存した分げつの成長制御

    大橋美和, 小島創一, 早川俊彦

    化学と生物　2016

    DOI： 10.1271/kagakutoseibutsu.54.867 　

  9. 1 イネ高親和型アンモニウムトランスポーター1;3の逆遺伝学的な解析(東北支部講演会,2014年度各支部会)

    藤田 貴之, 石塚 和, 石山 敬貴, 早川 俊彦, 山谷 知行, 山口 淳二, 小島 創一

    日本土壌肥料学会講演要旨集　(61)　246-246　2015/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  10. Identification of a specific combination of DNA elements in the promoter regions of ammonium responsive genes in Arabidopsis root

    KONISHI Noriyuki, ISHIYAMA Keiki, MARUYAMA Aoi, SAITO Masahide, MARU Ikumi, HAYAKAWA Toshihiko, YAMAYA Tomoyuki, KOJIMA Soichi

    日本植物生理学会年会要旨集　56th　2015

  11. 4-1-1 イネの根におけるAS分子種の生理的な役割と制御機構の解明(4-1 植物の多量栄養素,2014年度東京大会)

    大橋 美和, 石山 敬貴, 小島 創一, 早川 俊彦, 山谷 知行

    日本土壌肥料学会講演要旨集　(60)　47-47　2014/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  12. シロイヌナズナは2つの低親和型グルタミン合成酵素を使い分けて根でアンモニウムを同化する

    小西範幸, 石山敬貴, 丸郁美, 松岡香矢, 早川俊彦, 山谷知行, 小島創一

    日本植物生理学会年会要旨集　55th　2014

  13. シロイヌナズナNADH-グルタミン酸合成酵素の根におけるアンモニウム同化の重要性

    小西範幸, 松岡香矢, 丸郁美, 早川俊彦, 山谷知行, 小島創一

    日本植物生理学会年会要旨集　54th　2013

  14. シロイヌナズナサイトゾル型グルタミン合成酵素1;2は根でアンモニウムによって誘導されアンモニウムを同化する

    小西範幸, 早川俊彦, 山谷知行, 小島創一

    日本農芸化学会大会講演要旨集(Web)　2013　2013

    ISSN： 2186-7976

  15. イネ根における新規プロテインキナーゼ(OsACTPK1)のアンモニウム吸収制御への関与

    谷合彰子, 澤勇己, 小原実広, 吉成晃, 小島創一, 山谷知行, 早川俊彦

    日本植物生理学会年会要旨集　53rd　2012

  16. リン欠乏シロイヌナズナ根からのリンゴ酸およびクエン酸分泌に関わるトランスポーターの解析

    丸山隼人, 佐々木孝行, 小島創一, 和崎淳

    日本土壌肥料学会講演要旨集　57　(57)　66-66　2011/08/08

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  17. 土壌中難利用性リン利用能のシロイヌナズナ自然系統間比較解析

    古谷あゆ美, 丸山隼人, 小島創一, 岡崎圭毅, 信濃卓郎, 和崎淳

    日本植物生理学会年会要旨集　52nd　355　2011/03/11

    DOI： 10.14841/jspp.2011.0.0965.0 　

  18. ACTドメインを有する新規プロテインキナーゼ様タンパク質1(OsACTPK1)遺伝子破壊イネの根におけるアンモニウ吸収・同化

    谷合彰子, 澤勇己, 小原実広, 吉成晃, 小島創一, 山谷知行, 早川俊彦

    日本植物生理学会年会要旨集　52nd　2011

  19. A report for the 1st international symposium on the nitrogen nutrition of plants

    Soichi Kojima, Kuni Sueyoshi, Tomoyuki Yamaya

    Japanese Journal of Soil Science and Plant Nutrition　81　(6)　621-622　2010/12/05

    Publisher: 日本土壌肥料学会

  20. 9-5 イネにおけるグルタミン情報伝達系への関与が期待される新規ACTドメインプロテインキナーゼ様タンパク質4のリン酸化能解析(9.植物の多量栄養素,2010年度北海道大会)

    谷合 彰子, 澤 勇己, 西濱 夏海, 工藤 徹, 小島 創一, 山谷 知行, 早川 俊彦

    日本土壌肥料学会講演要旨集　(56)　55-55　2010/09/07

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  21. 9-8 サイトゾル型グルタミン合成酵素1;2遺伝子破壊がイネの収量に及ぼす影響(9.植物の多量栄養素,2010年度北海道大会)

    舟山 和宏, 小林(田渕) 真由美, 澤 勇己, 小島 創一, 早川 俊彦, 山谷 知行

    日本土壌肥料学会講演要旨集　(56)　56-56　2010/09/07

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  22. P9-10 シロイヌナズナ高親和型尿素輸送担体(AtDUR3)の翻訳後制御機構の探索とその基質特異性について(ポスター紹介,9.植物の多量栄養素,2010年度北海道大会)

    吉田 圭吾, 大岩 優貴, 松岡 香矢, 早川 俊彦, 山谷 知行, 小島 創一

    日本土壌肥料学会講演要旨集　(56)　66-66　2010/09/07

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  23. 9-11 イネのNADH-グルタミン酸合成酵素2遺伝子破壊変異体を用いた表現型の解析(9.植物の多量栄養素,2010年度北海道大会)

    田村 亘, 豊川 絢子, 田渕 真由美, 小島 創一, 早川 俊彦, 山谷 知行

    日本土壌肥料学会講演要旨集　(56)　57-57　2010/09/07

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  24. 9-19 シロイヌナズナ根のアンモニウム栄養に対する応答の分子遺伝学的な解析(9.植物の多量栄養素,2010年度北海道大会)

    小島 創一, 佐々木 和浩, 大岩 優貴, 松岡 香矢, 早川 俊彦, 佐藤 雅志, 山谷 知行

    日本土壌肥料学会講演要旨集　56　(0)　60-60　2010

    Publisher: 一般社団法人 日本土壌肥料学会

    DOI： 10.20710/dohikouen.56.0_60_1 　

    ISSN： 0288-5840

  25. 9-6 イネNADH依存性グルタミン酸合成酵素1(NADH-GOGAT1)破壊変異体の獲得と表現型の解析(9.植物の多量栄養素,2009年度京都大会)

    田村 亘, 日高 佑典, 田渕 真由美, 小島 創一, 早川 俊彦, 山谷 知行

    日本土壌肥料学会講演要旨集　(55)　66-66　2009/09/15

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  26. 9-9 液胞型アクアポリンの細胞膜アンモニウム輸送への寄与(9.植物の多量栄養素,2009年度京都大会)

    小島 創一, 早川 俊彦, Wiren Nicolaus von, 山谷 知行

    日本土壌肥料学会講演要旨集　(55)　67-67　2009/09/15

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  27. 9-3 シロイヌナズナの高親和性尿素輸送担体の翻訳後制御機構の探索(9.植物の多量栄養素,2009年度京都大会)

    吉田 圭吾, Bohner Anne, 早川 俊彦, 山谷 知行, Wiren Nicolaus von, 小島 創一

    日本土壌肥料学会講演要旨集　(55)　65-65　2009/09/15

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  28. 9-12 イネ根におけるGlnセンサー候補OsACTPK遺伝子族の発現特性とOsACTPK4遺伝子破壊イネの解析(9.植物の多量栄養素,2009年度京都大会)

    澤 勇己, 西濱 夏海, 小島 創一, 山谷 知行, 早川 俊彦

    日本土壌肥料学会講演要旨集　(55)　68-68　2009/09/15

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  29. III-4 アンモニウム膜輸送の分子機構(III 養分吸収の分子メカニズム-どこまでわかったか、何がまだわかっていないか-,シンポジウム,2008年度愛知大会)

    小島 創一, Yuanc Lixing, Loquec Dominique, 高橋 秀樹, Wiren Nicolaus von

    日本土壌肥料学会講演要旨集　(54)　222-222　2008/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  30. 9-35 アラビドプシス尿素輸送担体の生理機能(9.植物の無機栄養,2008年度愛知大会)

    小島 創一, Bohner Anne, 早川 俊彦, 山谷 知行, Wiren Nicolaus von

    日本土壌肥料学会講演要旨集　(54)　81-81　2008/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  31. リン欠乏条件下で形成されるクラスター根における酸性ホスファターゼの組織化学的局在性

    丸山隼人, 和崎淳, 小島創一, KANDELER Ellen, 伊藤進, 信濃卓郎, 大崎満

    日本植物生理学会年会要旨集　49th　331　2008/03/15

    DOI： 10.14841/jspp.2008.0.0912.0 　

  32. 植物のアンモニア態窒素吸収機構の機能分担を解明

    Soichi Kojima, Hideki Takahashi

    BRAIN techno news　(126)　20-24　2008/03/15

  33. Increased Rubisco Content in Transgenic Rice Transformed with “Sense” rbcS Gene

    Yuji Suzuki, Maki Ohkubo, Hanako Hatakeyama, Keiko Ohashi, Ryuichi Yoshizawa, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya, Tadahiko Mae, Amane Makino

    Photosynthesis. Energy from the Sun　885-888　2008

  34. Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene

    Y. Suzuki, M. Ohkubo, H. Hatakeyama, K. Ohashi, R. Yoshizawa, S. Kojima, T. Hayakawa, T. Yamaya, T. Mae, A. Makino

    PHOTOSYNTHESIS RESEARCH　91　(2-3)　232-233　2007/02

    ISSN： 0166-8595

  35. 10-8 イネおけるrbcS遺伝子の過剰発現による葉身 Rubisco 含量の増加(10. 植物の代謝, 2006年度秋田大会講演要旨)

    鈴木 雄二, 大久保 真葵, 畠山 華子, 大橋 (兼子)敬子, 小島 創一, 早川 俊彦, 山谷 知行, 前 忠彦, SAGE Rowan F., 牧野 周

    日本土壌肥料学会講演要旨集　(52)　86-86　2006/09/05

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  36. Molecular mechanisms of urea transport in plants

    S. Kojima, A. Bohner, N. von Wiren

    JOURNAL OF MEMBRANE BIOLOGY　212　(2)　83-91　2006/09

    DOI： 10.1007/s00232-006-0868-6 　

    ISSN： 0022-2631

    eISSN： 1432-1424

  37. Identification of glutamine synthetase (GS) and glutamate synthase (GOGAT) responsible for assimilation of exogenous NH4+ in Arabidopsis root

    K Ishiyama, E Inoue, A Takahashi-Watanbe, S Kojima, T Yamaya, H Takahashi

    PLANT AND CELL PHYSIOLOGY　44　S40-S40　2003

    ISSN： 0032-0781

  38. 10-4 アラビドプシスNADH-グルタミン酸合成酵素の組織局在に関する解析(10.植物の代謝)

    小島 創一, 早川 俊彦, 山谷 知行

    日本土壌肥料学会講演要旨集　(48)　72-72　2002/03/25

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  39. Cis-elements that regulate vascular-bundle specific expression of NADH-GOGAT gene in rice.

    S Kojima, T Hayakawa, T Yamaya

    PLANT AND CELL PHYSIOLOGY　43　S127-S127　2002

    ISSN： 0032-0781

  40. Investigation of cis element that regulates tissue-specific expression of gene for rice NADH-GOGAT :

    KOJIMA Soichi, HAYAKAWA Toshihiko, YAMAYA Tomoyuki

    Plant and cell physiology　42　s186　2001

    Publisher: Japanese Society of Plant Physiologists

    ISSN： 0032-0781

  41. 10-7 イネNADH依存性グルタミン酸合成酵素遺伝子及びサイトゾル型グルタミン合成酵素遺伝子のプロモーター領域の解析(10.植物の代謝)

    小島 創一, 半澤 咲子, 野崎 雪音, 早川 俊彦, 山谷 知行

    日本土壌肥料学会講演要旨集　(46)　97-97　2000/03/25

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN： 0288-5840

  42. Tissue specific and nitrogen inducible expression of rice NADH-GOGAT gene promoter in transgenic rice plants

    KOJIMA Soichi, KIMURA Michiko, HAYAKAWA Toshihiko, YAMAYA Tomoyuki

    40　s141-s141　1999/03

    ISSN： 0032-0781

  43. ANALYSIS OF NADH DEPENDENT GLUTAMATE SYNTHASE PROMOTER IN TRANSGENIC RICE PLANTS

    KOJIMA Soichi, TAKAHASHI Fumie, GOTO Satoshi, HAYAKAWA Toshihiko, YAMAYA Tomoyuki

    39　S38-S38　1998/05

    ISSN： 0032-0781

Show all ︎Show first 5

Books and Other Publications 2

  1. テイツ・ザイガー植物生理学

    編集)西谷和彦, 島崎研一郎, 分担, 小島創一

    講談社サイエンティフィック　2016

  2. Ammonium absorption and assimilation in plants

    Soichi Kojima, Toshihiko Hayakawa, Nicolaus von Wirén, Tomoyuki Yamaya

    Research Signpost　2010

Presentations 129

  1. 植物の尿素輸送担体の機能

    小島創一

    植物の栄養研究会　2023/09/06

  2. レガシーデータを活用したダイズの開花期を制御する遺伝子座の探索

    菅波眞央, 高橋秀和, 二瓶直登, 吉田英樹, 小島創一, 佐藤郁恵, 山本英司, 吉田晋弥, 渡辺正夫, 松田幹, 松岡信

    第144回日本育種学会　2023/09/16

  3. 複数形質のGWASの比較によるQTLの特異性・多面性の評価

    菅波眞央, 高橋秀和, 二瓶直登, 吉田英樹, 小島創一, 佐藤郁恵, 渡辺正夫, 松田幹, 松岡信

    2023年度土壌肥料学会　2023/09/14

  4. シロイヌナズナのアンモニウム応答性グルタミン・グルタミン酸合成酵素の発現制御機構の解明

    小島創一

    2023年度土壌肥料学会　2023/09/14

  5. イネ細胞質型グルタミン合成酵素1;2(OsGS1;2)が根の微生物群集に与える影響の解析

    小澤元, 番場大, 橋本駿, 佐藤修正, 小島創一

    日本植物学会第87回大会　2023/09/04

  6. 日本ダイズ品種で構成されたGWAS集団の構築

    菅波眞央, 高橋秀和, 二瓶直登, 吉田英樹, 小島創一, 渡辺正夫, 松田幹, 松岡信

    作物学会東北談話会講演会　2023/08/21

  7. Coregulation of glutamine synthetase 1;2 and NADH dependent glutamate synthase gene expression in Arabidopsis roots in response to ammonium supply International-presentation International-coauthorship

    Soichi Kojima, Hideki Takahashi, Keiki Ishiyama

    The 33rd International Conference on Arabidopsis Research　2023/06/06

  8. Ammonium-induced regulation of GS/GOGAT gene in Arabidopsis root

    Soichi Kojima, Keiki Ishiyama

    The 64th annual meeting of the Japanese Society of Plant Physiologist　2023/03/13

  9. Effective use of legacy data for GWAS to improve reliability of QTL detection in rice

    Mao Suganami, Soichi Kojima, Fanmiao Wang, Hideki Yoshida, Kotaro Miura, Yoichi, Morinaka, Masao Watanabe, Tsukasa Matsuda, Eiji Yamamoto, Makoto Matsuoka

    2023/03/13

  10. 分げつが少ないイネ細胞質型グルタミン合成酵素 1;2 変異 体の密植環境における生産性の解析

    小島創一

    植物の栄養研究会　2022/12/09

  11. 日本のダイズ品種を中心としたGWAS解析集団の構築

    菅波眞央, 高橋秀和, 吉田英樹, 二瓶直登, 石川大太郎, 牧雅康, 佐藤郁恵, 平山孝, 遠藤あかり, 吉田晋弥, 小島創一, 渡辺正夫, 松田幹, 松岡信

    日本作物学会第254回講演会

  12. The physiological function of cytosolic glutamine synthetase 1;3 in germination and grain-filling.

    Soichi Kojima, Marcel Beier, Keiki Ishiyama, Toshihiko Hayakawa

    Annual Meeting for Japanese Society of Soil Science and Plant Nutrition 2022 Tokyo　2022/09/15

  13. ゲノム育種の加速化に有効なレガシーデータを活用したGWAS

    菅波眞央, 小島創一, 吉田英樹, Fanmiao Wang, 森中洋一, 渡辺正夫, 松田幹, 松岡信

    日本作物学会第253回講演会　2022/03/27

  14. Search for “NH4+-responsive region” in sequences of OsAS1 and OsRBCS promoters in rice

    Dong-Kyung Yoon, Shiori Sato, Eri Kondo, Hiroyuki Ishida, Tadahiko Mae, Amane Makino, Soichi Kojima, Keiki Ishiyama

    The 63rd Annual Meeting of the Japanese Society of Plant Physiologists　2022/03/22

    More details Close

    In Arabidopsis, NH4+ absorbed from the roots is assimilated by GLN1;2 which is a molecular species of cytosolic glutamine synthetase. In previous study, we found that GLN1;2 mRNA has transiently increased by supplying NH4+ to the roots. In addition, cis-element region named “NH4+ -responsive region (ARR)”, in its promoter region for enhancing the transcriptional activity of the GLN1;2 gene was found by promoter deletion analysis. The ARR has binding DNA sequences for transcription factors, such as WRKY, bHLH, Dof and some enhancers.  In current study, we found DNA sequences similar to ARR in the Asparagine synthetase1 (OsAS1) and Rubisco small subunit genes (OsRBCS2-5) promoter regions in rice, in silico. In this work, we investigated the expression levels of OsAS1 and OsRBCS genes in roots and leaf blades, respectively, of rice plants after the NH4+ treatment. Both OsAS1 and OsRBCS3 were drastically responsive to NH4+ by increasing their mRNAs within 24 hrs after ammonium supply. We are currently working on producing transgenic rice introduced by deleted promoter of OsAS1 and OsRBCS3 fused GUS genes, respectively, trying to confirm the presence of ARRs in their promoter regions.

  15. A vacuolar glutamine efflux transporter in rice

    ○Toshihiko Hayakawa, Saori Ogasawara, Masataka Ezaki, Kuni Sueyoshi, Shunya Saito, Toru Kudo, Soichi Kojima, Nobuyuki Uozumi

    The 63rd Annual Meeting of the Japanese Society of Plant Physiologists　2022/03/22

    More details Close

    The growth of ammonium-preferring paddy rice depends on root ammonium assimilation into glutamine (Gln) and subsequent root-to-shoot allocation of Gln; however, little is known about the mechanism of Gln storage in roots. In this study, we show that the rice amino acid transporter-like 6 (OsATL6) exports Gln to the root vacuoles under replete ammonium. OsATL6 was expressed along with glutamine synthetase1;2 and NADH-glutamate synthase1, key ammonium-assimilatory genes, in sufficient ammonium-fed WT roots. Under replete ammonium, seedlings of OsATL6 knockdown mutants by Tos17 insertion exhibited lower root Gln content but higher Gln concentration in the xylem sap and greater shoot growth, compared with segregants lacking the Tos17 insertion. The transiently expressed mRFP-OsATL6 was localized at tonoplasts in onion cells. When OsATL6 was expressed in Xenopus laevis oocytes, Gln efflux from the cell into the acidic bath solution increased. OsATL6 transiently accumulated in sclerenchyma, pericycle, and vascular parenchyma cells of sufficient ammonium-fed WT roots. These findings suggest that OsATL6 temporarily stores excess Gln in root vacuoles before it can be translocated to shoot.

  16. The function of high-affinity urea transporters in nitrogen-deficient conditions

    Soichi Kojima, Marcel Pascal Beier

    The 63rd Annual Meeting of the Japanese Society of Plant Physiologists　2022/03/22

    More details Close

    Urea is the most widely used nitrogenous fertilizer worldwide and is an important nitrogen source for plant growth. Although urea is a common fertilizer, its direct uptake is limited, as the bacterial urease degrades urea into ammonium in soil. In addition to exogenously delivered urea from the soil, urea is endogenously synthesized during the secondary metabolism of nitrogen. We investigated the contribution of a urea transporter, DUR3, to rice production using a reverse genetic approach combined with localization studies. Tos17 insertion lines for DUR3 showed a 50% yield reduction in hydroponic culture, and a 26.2% yield reduction in a paddy field, due to decreased grain filling. As the shoot biomass was not reduced, insertion lines were disordered not only in nitrogen acquisition but also in nitrogen allocation. Isotope tracing indicated that DUR3 insertion lines accumulated nitrogen in leaves and could not sufficiently translocate nitrogen to their panicles. DUR3 promoter activity was high in the vascular bundles of senescent leaves. These results suggest that under nitrogen-deficient and field conditions, DUR3 contributes to the translation of nitrogen and rice.

  17. NADH依存性グルタミン酸合成酵素遺伝子の遺伝的な相互作用の解析

    松村剛士, 宇野享, 田島亮介, 小島創一

    日本土壌肥料学会2021年度北海道大会　2021/09/14

  18. The composition of the lignocellulose is dependent on the amino acid metabolism in the rice International-presentation

    Yoshiki Shimabarashi, Soichi Kojima

    The 7th International Conference on Plant Cell Wall Biology (PCWB2021)　2021/06/30

  19. Changes of rice grain nutrient in mutant lines for amino acid metabolism

    Soichi Kojima

    The 2021 Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry　2021/03/19

  20. The growth and yield of double insertion line for NADH-GOGAT1 and NADH-GOGAT2 in rice

    Matsumura Tsuyoshi, Tajima Ryosuke, Kojima Soichi

    The 62nd annual meeting of Japanese Society of Plant Physiologists　2021/03/21

  21. Cellulose concentration in Tos17 insertion lines for amino acid metabolism

    Yoshiki Shimabarashi, Soichi Kojima

    2020/11/07

  22. Lignin concentration is slightly dependent on glutamine synthesis

    Yoshiki Shimabarashi, Soichi Kojima

    2020/11/06

  23. 尿素トランスポーターDUR3はイネの収量生産に寄与する

    Marcel Beier, 小島創一

    第15回トランスポーター研究会　2020/10/15

  24. 細胞質型グルタミン合成酵素1;2を欠損するシロイヌナズナの根のアンモニウムに対する形態の解析

    北村優太, 小島創一

    日本土壌肥料学会2020年度岡山大会　2020/09/10

  25. イネのGS/GOGAT変異に伴う木質バイオマスの変動の解析

    嶋林佳生, 小島創一

    日本土壌肥料学会2020年度岡山大会　2020/09/10

  26. The localization of ammonium transporter in vascular tissue of tomato leaves

    Soichi Kojima

    2019/09/26

  27. Changes of amino acid composition in the grains of Tos17 insertion line in asparagine synthetase

    Fumi Imagawa, Soichi Kojima

    2019/03/16

  28. Reverse genetics approach to characterize a function of amino acid metabolism in the quality and germination of rice grains International-presentation

    KOJIMA Soichi

    6th Plant Dormancy Symposium 2018　2018/10/23

  29. イネのアミノ酸合成酵素の機能を欠損する変異体の種子成分の解析

    皆川春香, 今川富美, 早川俊彦, 小島創一

    2018年度北海道支部・東北支部合同支部会　2018/09/22

  30. シロイヌナズナ環境型のアンモニウム態窒素栄養への応答の比較

    小島創一

    日本土壌肥料学会2017年度仙台大会　2017/09/05

  31. シロイヌナズナの窒素利用におけるオーキシン情報伝達の役割の解析

    齋藤雅英, 小西範幸, 菅野圭一, 山谷知行, 小島創一

    日本土壌肥料学会2017年度仙台大会　2017/09/05

  32. Contributions of two cytosolic glutamine synthetase isozymes to ammonium assimilation in Arabidopsis roots International-presentation

    Noriyuki Konishi, Keiki Ishiyama, Marcel Pascal Beier, Eri Inoue, Keiichi Kanno, Tomoyuki Yamaya, Hideki Takahashi, Soichi Kojima

    International Plant Nutrition Colloquium　2017/08/21

  33. Cytosolic glutamine synthetase1;2 contributes to nitrogen-dependent biosynthesis of cytokinin required for rice tillering International-presentation

    Miwa Ohashi, Keiki Ishiyama, Soichi Kojima, Mikiko Kojima, Hitoshi Sakakibara, Tomoyuki Yamaya, Toshihiko Hayakawa

    International Plant Nutrition Colloquium　2017/08/21

  34. シロイヌナズナのアンモニウム応答性グルタミン合成酵素は根のアンモニウム輸送を抑制する

    小島創一

    第12回トランスポーター研究会　2017/07/08

  35. シロイヌナズナ根のアンモニウム同化におけるグルタミン合成酵素アイソザイムの機能分担

    小西範幸, 石山敬貴, 菅野圭一, 小島創一

    第58回日本植物生理学会年会　2017/03/16

  36. サイトゾル型グルタミン合成酵素1;2を欠損したイネ変異体の分げつの減少はアスパラギンよりもグルタミンの利用可能量の減少に起因する

    大橋美和, 石山敬貴, 小島創一, 小西範幸, 宮尾光恵, 山谷知行, 早川俊彦

    第58回日本植物生理学会年会　2017/03/16

  37. シロイヌナズナの根におけるアンモニウムの初期同化

    小西範幸, 石山敬貴, 菅野圭一, 早川俊彦, 山谷知行, 小島創一

    第45回根研究集会　2016/09/30

  38. シロイヌナズナ根のサイトゾル型グルタミン合成酵素アイソザイムの機能解析

    小西範幸, 石山敬貴, 菅野圭一, 小島創一

    第2回植物の栄養研究会　2016/09/02

  39. Lack of cytosolic glutamine synthetase1;2 reduced the availability of glutamine and sucrose for axillary bud outgrowth in the rice seedling.

    Miwa Ohashi, Keiki Ishiyama, Miyako Kusano, Atsushi Fukushima, Soichi Kojima, Tomoyuki Yamaya, Toshihiko Hayakawa

    第57回日本植物生理学会年会　2016/03/18

  40. High-affinity urea transporter DUR3 is involved in rice productivity under nitrogen deficient condition

    Takayuki Fujita, Kazuhiro Sasaki, Keiichi Kanno, Tomoyuki Yamaya, Soichi Kojima

    第57回日本植物生理学会年会　2016/03/18

  41. シロイヌナズナのサイトゾル型グルタミン合成酵素GLN1;2に依存的なアンモニウムの同化

    小西範幸, 石山敬貴, 山谷知行, 小島創一

    第57回日本植物生理学会年会　2016/03/18

  42. シロイヌナズナの窒素栄養利用におけるオーキシン情報伝達の役割の解明

    齋藤雅英, 小西範幸, 菅野圭一, 小島創一

    第57回日本植物生理学会年会　2016/03/18

  43. A serine/threonine/tyrosine protein kinase, OsACTPK1, mediates down-modulation of the high-affinity ammonium uptake by rice roots under high ammonium supply

    Beier Marcel Pascal, 石澤仁, 吉田喜紀, 小島創一, 早川俊彦

    第57回日本植物生理学会年会　2016/03/18

  44. 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に与える影響の解析

    横浜諒, 和田慎也, 菅野圭一, 小島創一, 山谷知行, 牧野周, 石田宏幸

    第57回日本植物生理学会年会　2016/03/18

  45. シロイヌナズナ根で発現する遺伝子群のアンモニウム応答に必要な領域の特定

    小西範幸, 石山敬貴, 丸山葵, 齋藤雅英, 丸郁美, 早川俊彦, 山谷知行, 小島創一

    第56回日本植物生理学会年会　2015/03/16

  46. Stimulation of axillary buds elongation by metabolite and cytokinin in rice

    Miwa Ohashi, Keiki Ishiyama, Miyako Kusano, Atsushi Fukushima, Mikiko Kojima, Soichi Kojima, Toshihiko Hayakawa, Hitoshi Sakakibara, Tomoyuki Yamaya

    第56回日本植物生理学会年会　2015/03/16

  47. イネの根におけるAS分子種の生理的な役割と制御機構の解明

    大橋美和, 石山敬貴, 小島創一, 早川俊彦, 山谷知行

    日本土壌肥料学会2014年度東京大会　2014/09/09

  48. 高親和型尿素輸送担体が破壊されたイネの収量の解析

    藤田貴之, 石山敬貴, 早川俊彦, 山谷知行, 小島創一

    第32回日本植物細胞分子生物学会大会　2014/08/21

  49. イネ高親和型アンモニウムトランスポーター1;3の逆遺伝学的な解析

    藤田貴之, 石塚和, 石山敬貴, 早川俊彦, 山谷知行, 山口淳二, 小島創一

    土壌肥料学会東北支部会　2014/07/07

  50. イネGS1;2あるいはNADH-GOGAT1欠損変異体における分げつ数の減少とストリゴラクトンとの関連性の解明

    大橋美和, 石山敬貴, 花田篤志, 小島創一, 早川俊彦, 瀬戸義哉, 山口信次郎, 山谷知行

    第55回日本植物生理学会年会　2014/03/18

  51. シロイヌナズナは2つの低親和型グルタミン合成酵素を使い分けて根でアンモニウムを同化する

    小西範幸, 石山敬貴, 丸郁美, 松岡香矢, 早川俊彦, 山谷知行, 小島創一

    第55回日本植物生理学会年会　2014/03/18

  52. The proper use of glutamine produced by cytosolic GS1;1 is critical for metabolic and organelle homeostasis in rice.

    Kusano M, Fukushima A, Funayama K, Tabuchi-Kobayashi M, Nishizawa T, Kobayashi M, Wakazaki M, Sato M, Toyooka M, Osanai-Kondo K, Utsumi Y, Seki S, Kojima S, Saito S, Yamaya T

    第55回日本植物生理学会年会　2014/03/18

  53. The study of two cytosolic glutamine synthetase isoforms of rice using reverse genetic, metabolite and transcript profiling approaches and microscopic analysis. International-presentation

    Kusano M, Fukushima A, Funayama K, Tabuchi-Kobayashi M, Nishizawa T, Kobayashi M, Waakazaki M, Sato M, Toyooka K, Osanai-Kondo K, Utsumi Y, Seki M, Kojima S, Yamaya T, Saito K

    Second International Symposium on the Nitrogen Nutrition of Plants　2013/11/18

  54. Rice novel protein kinase involved in regulation of ammonium uptake into roots under high concentration of external ammonium. International-presentation

    Hayakawa T, Obara M, Taniai A, Sawa Y, Ishizawa J, Kojima S, Yamaya T

    Second International Symposium on the Nitrogen Nutrition of Plants　2013/11/18

  55. Ubiquitin domain containing protein interacts with RF2, a restorer of fertility of LD-type cytoplasmic male sterility in rice International-presentation

    Fujii, Shinya, Kazama, Tomohiko, Ito, Yukihiro, Kojima, Soichi, Toriyama, Kinya

    7th International Rice Genetics Symposium　2013/11/05

  56. イネLD型CMS系統における稔性回復因子RF2と 相互作用するUbiquitin domain containing proteinの解析

    藤井慎也, 風間智彦, 小島創一, 伊藤幸博, 鳥山欽哉

    2013年日本育種学会秋季大会（第124回講演会）　2013/10/12

  57. シロイヌナズナサイトゾル型グルタミン合成酵素1;2は根でアンモニウムによって誘導されアンモニウムを同化する

    小西範幸, 早川俊彦, 山谷知行, 小島創一

    日本農芸化学会2013年度仙台大会　2013/03/25

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    Arabidopsis cytosolic glutamine synthetase 1;2 is induced by ammonium supply and assimilates ammonium in root

  58. シロイヌナズナNADH-グルタミン酸合成酵素の根におけるアンモニウム同化の重要性

    小西範幸, 松岡香矢, 丸郁美, 早川俊彦, 山谷知行, 小島創一

    第54回日本植物生理学会年会　2013/03/21

  59. シロイヌナズナ環境型におけるアンモニウム栄養利用の遺伝的な差異

    松岡香矢, 佐々木和浩, 大岩優貴, 丸郁美, 早川俊彦, 佐藤雅志, 山谷知行, 小島創一

    第54回日本植物生理学会年会　2013/03/21

  60. イネLD型細胞質雄性不稔系統における稔性回復因子RF2と相互作用するタンパク質の探索

    藤井慎也, 風間智彦, 伊藤幸博, 小島創一, 鳥山欽哉

    第30回日本植物細胞分子生物学会大会　2012/08/03

  61. Evidence to support a function of glutamine synthetase1;2 in the primary assimilation of ammonium ions in rice roots

    Kazuhiro Funayama, Mayumi Tabuchi-Kobayashi, Yuki Sawa, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya

    第53回日本植物生理学会年会　2012/03/16

  62. イネ根における新規プロテインキナーゼ(OsACTPK1) のアンモニウ吸収制御への関与

    谷合彰子, 澤勇己, 小原実広, 吉成晃, 小島創一, 山谷知行, 早川俊彦

    第53回日本植物生理学会年会　2012/03/16

  63. 酵母スプリットユビキチン法によるアンモニウム輸送担体と細胞質型グルタミン合成酵素の相互作用の解析

    松岡香矢, 大岩優貴, 早川俊彦, 山谷知行, 小島創一

    第53回日本植物生理学会年会　2012/03/16

  64. Reduction of the primary assimilation of ammnonium ions and panicle number in rice mutants lacking cytosolic glutamine synthetase1;2. International-presentation

    Kazuhiro Funayama, Mayumi Tabuchi-Kobayashi, Yuki Sawa, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya

    International Symposium “Strategies of Plants against Global Environmental Change”　2011/12/08

  65. Interplay between ammonium transporter and glutamine synthethase in Arabidopsis. International-presentation

    Kaya Tanaka-Matsuoka, Yuki Oiwa, Toshihiko Hayakawa, Tomoyuki Yamaya, Soichi Kojima

    International Symposium “Strategies of Plants against Global Environmental Change”　2011/12/08

  66. Analysis of malate and citrate transporters induced in roots of phosphorus deficient Arabidopsis thaliana International-presentation

    Hayato Maruyama, Takayuki Sasaki, Keiki Okazaki, Soichi Kojima, Takuro Shinano, Jun Wasaki

    Rhizosphere 3　2011/09/25

  67. リン欠乏シロイヌナズナ根からのリンゴ酸およびクエン酸分泌に関わるトランスポーターの解析

    丸山隼人, 佐々木孝行, 小島創一, 和崎淳

    日本土壌肥料学会2011年度つくば大会　2011/08

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  68. Analysis of malate transporters induced in roots of phosphorus deficient or aluminum stressed Arabidopsis thaliana. International-presentation

    Maruyama, H, Sasaki, T, Kojima, S, Wasaki, J

    International Conference on Arabidopsis Research 2011　2011/06/22

  69. シロイヌナズナ高親和型尿素輸送担体（AtDUR3）の翻訳後制御機構の探索とその基質特異性について

    吉田圭吾, 大岩優貴, 松岡香矢, 早川俊彦, 山谷知行, 小島創一

    The 2010 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2010/09/07

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  70. シロイヌナズナ根のアンモニウム栄養に対する応答の分子遺伝学的な解析

    小島創一, 佐々木和浩, 大岩優貴, 松岡香矢, 早川俊彦, 佐藤雅志, 山谷知行

    The 2010 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2010/09/07

  71. イネのNADH- グルタミン酸合成酵素2 遺伝子破壊変異体を用いた表現型の解析

    田村 亘, 豊川絢子, 田渕真由美, 小島創一, 早川俊彦, 山谷知行

    The 2010 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2010/09/07

  72. サイトゾル型グルタミン合成酵素1;2 遺伝子破壊がイネの収量に及ぼす影響

    舟山和宏, 小林(田渕, 真由美, 澤 勇己, 小島創一, 早川俊彦, 山谷知行

    The 2010 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2010/09/07

  73. イネにおけるグルタミン情報伝達系への関与が期待される新規ACT ドメインプロテインキナーゼ様タンパク質4 のリン酸化能解析

    谷合彰子, 澤 勇己, 西濱夏海, 工藤 徹, 小島創一, 山谷知行, 早川俊彦

    The 2010 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2010/09/07

  74. Localized ammonium supply increases lateral root branching International-presentation

    Joni Lima, Soichi Kojima, Hideki Takahashi, Nicolaus von Wirén

    Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants　2010/07/26

  75. Reverse genetic approach unravels the physiological functions of NADH-dependent glutamate synthase1 (NADH-GOGAT1) in ammonium assimilation in the root and productivity of rice plant International-presentation

    Wataru Tamura, Yusuke Hidaka, Mayumi Tabuchi, Soichi Kojima, Mikiko Kojima, Toshihiko Hayakawa, Hitoshi Sakakibara, Tomoyuki Yamaya

    Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants　2010/07/26

  76. Cytosolic glutamine synthetase 1;2 is one of a key enzymes in ammonium assimilation in root of rice plant International-presentation

    Kazuhiro Funayama, Mayumi Tabuchi, Yuki Sawa, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya

    Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants　2010/07/26

  77. Changes of root system by polyamine supply is dependent on high-affinity urea transporter International-presentation

    Keigo Yoshida, Yuki Oiwa, Kaya Tanaka-Matsuoka, Toshihiko Hayakawa, Tomoyuki Yamaya, Nicolaus von Wirén, Soichi Kojima

    Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants　2010/07/26

  78. Rice vacuolar membrane-localized amino acid transporter, OsATA1;2, transports glutamine International-presentation

    Toshihiko Hayakawa, Ryusuke Ishida, Keisuke Kobayashi, Kuni Sueyoshi, Toru Kudo, Yoko Shiroto, Yoshitsugu Hongo, Soichi Kojima, Tomoyuki Yamaya

    Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants　2010/07/26

  79. Interplay between ammonium transport and auxin signaling International-presentation

    Soichi Kojima, Yuki Oiwa, Desirée Schüssler, Keigo Yoshida, Kaya Tanaka-Matsuoka, Toshihiko Hayakawa, Tomoyuki Yamaya, Nicolaus von Wirén

    Nitrogen2010, 1st International Symposium on the Nitrogen Nutrition of Plants　2010/07/26

  80. シロイヌナズナにおけるアンモニウムの輸送とグルタミン代謝の協調

    小島創一, 石山敬貴, 早川俊彦, 山谷知行, Nicolaus von Wiré, 高橋秀樹

    第5回トランスポーター研究会　2010/07/10

  81. Systemic and local root development to localized putrescine supply is dependent on high-affinity urea transporter International-presentation

    Keigo Yoshida, Yuki Oiwa, Kaya Tanaka-Matsuoka, Toshihiko Hayakawa, Tomoyuki Yamaya, Nicolaus von Wirén, Soichi Kojima

    21st International Conference on Arabidopsis Research　2010/06/06

  82. シロイヌナズナ環境型のアンモニウム栄養に対する応答

    大岩優貴, 佐々木和浩, 吉田圭吾, 松岡香矢, 早川俊彦, 佐藤雅志, 山谷知行, 小島創一

    The 2010 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2010/03/17

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  83. シロイヌナズナ高親和型尿素輸送担体の相互作用因子の探索

    吉田圭吾, 大岩優貴, 松岡香矢, 早川俊彦, 山谷知行, 小島創一

    The 2010 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2010/03/17

  84. イネにおけるNADH依存性グルタミン酸合成酵素(NADH-GOGAT)1及び2遺伝子の破壊変異体の獲得とその表現型

    田村亘, 豊川絢子, 日高佑典, 田渕真由美, 小島創一, 早川俊彦, 山谷知行

    The 2010 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2010/03/17

  85. 植物の窒素栄養を運ぶトランスポーターの研究

    小島創一

    第4回先端農学セミナー　2009/11/20

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  86. シロイヌナズナの高親和尿素輸送担体の翻訳後制御機構の探索

    吉田圭吾, Anne Bohner, 早川俊彦, 山谷知行, Nicolaus von Wiren, 小島創一

    The 2009 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2009/09/15

  87. イネ根におけるGlnセンサー候補OsACTPK遺伝子族の発現特性とOsACTPK4遺伝子破壊イネの解析

    澤, 西濱, 小島, 山谷, 早川

    The 2009 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2009/09/15

  88. 液胞型アクアポリンの細胞膜アンモニウム輸送への寄与

    小島, 早川, von Wiren, 山谷

    The 2009 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2009/09/15

  89. イネNADH依存性グルタミン酸合成酵素1(NADH-GOGAT1)破壊変異体の獲得と表現型の解析

    田村, 日高, 田渕, 小島, 早川, 山谷

    The 2009 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2009/09/15

  90. 植物の栄養吸収に関する研究の国際的な競争

    第二回先端農学センターシンポジウム　2009/06/25

  91. シロイヌナズナ高親和型尿素輸送担体の生理機能と活性制御機構

    吉田圭吾, Anne Bohner, 早川俊彦, 山谷知行, Nicolaus von Wiren, 小島創一

    The 2009 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2009/03/21

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  92. NADH-依存性グルタミン酸合成酵素1(NADH-GOGAT1)機能欠損イネの獲得および生理学的解析

    日高佑典, 田村亘, 小島創一, 早川俊彦, 榊原均, 山谷知行

    The 2009 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2009/03/21

  93. イネにおけるACTドメインリピートタンパク質9(OsACR9)の制御下のグルタミン応答性遺伝子群候補とOsACR9の相互作用因子候補の探索

    河合亜希子, 工藤徹, 小島創一, 山谷知行, 早川俊彦

    The 2009 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2009/03/21

  94. 植物におけるアンモニウム輸送担体と根の形成

    第43回Plant Science Seminar　2008/11/25

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  95. 低窒素条件のイネ根で発現誘導されるアンモニウムトランスポーターと尿素トランスポーターの機能解析

    小島創一, 田渕真由美, 田村亘, 早川俊彦, 山谷知行

    重要形質領域オータムミーティング　2008/10/16

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  96. アラビドプシス尿素輸送担体の生理機能

    小島 創一, Anne Bohner, 早川 俊彦, 山谷 知行, Nicolaus von Wirén

    The 2008 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2008/09/16

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  97. アンモニウム膜輸送の分子機構

    小島創一, Lixing Yuan, Dominique Loqué, 高橋秀樹, Nicolaus von Wirén

    The 2008 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2008/09/16

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  98. Interplay between ammonia transport at tonoplast and ammonium transport at plasma membrane in a root of Arabidopsis thaliana.

    Kojima S, Loqué D, Bohner A, Hayakawa T, Yamaya T, von Wirén N

    第四回特定領域研究植物の環境適応戦略としてのオルガネラ分化ワークショップ　2008/08/20

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  99. Regulation of ammonium uptake and metabolism in plants International-presentation

    Takahashi H, Kojima S

    Japan-Korea Symposium - Plant Growth and Signal Transduction　2008/06/09

  100. Ammonium response of GLN1;2, glutamine synthetase in Arabidopsis roots.

    Kojima S, Ishiyama K, Inoue E, Yamaya T, Takahashi H

    The 2008 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2008/03

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  101. Histochemical localization of acid phosphatase incluster roots formed under P deficient conditions

    Hayato Maruyama, Jun Wasaki, Soichi Kojima, Ellen Kandeler, Susumu Ito, Takuro Shinano, Mitsuru Osaki

    The 2008 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2008/03

  102. 高等植物における高親和型尿素トランスポーターの生理機能

    Kojima S, Bohner A, Takahashi H, von Wirén N

    特定領域研究「植物の養分吸収と循環系」第二回ワークショップ　2007/12

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  103. AtDUR3 represents the major transporter for high-affinity urea transport across the plasma membrane of nitrogen-deficient Arabidopsis roots International-presentation

    Bohner A, Kojima S, Yuan L, von Wiren N

    Nitrogen 2007. An international symposium on the nitrogen nutrition of plants.　2007/07/27

  104. Is ammonium a sensed nitrogen form in planta? International-presentation

    Lima J, Yuan L, Kojima S, von Wiren N

    Nitrogen 2007. An international symposium on the nitrogen nutrition of plants.　2007/07/27

  105. The organization of high-affinity ammonium uptake in Arabidopsis roots. International-presentation

    Yuan L, Loque D, Kojima S, Rauch S, Ishiyama K, Takahashi H, von Wiren N

    Nitrogen 2007. An international symposium on the nitrogen nutrition of plants.　2007/07/27

  106. Unraveling mechanisms underlying the ammonium-responsive expression of glutamine synthetase 1;2 (GLN1;2) in Arabidopsis roots International-presentation

    Kojima S, Ishiyama K, Inoue E, Yamaya T, Takahashi H

    Nitrogen 2007. An international symposium on the nitrogen nutrition of plants.　2007/07/27

  107. The organization of high-affinity ammonium uptake in Arabidopsis roots relies on the isoform-specific localization and biochemical properties of AMT1-type transporters. International-presentation

    Yuan L, Loque D, Kojima S, Rauch S, Ishiyama K, Takahashi H, von Wiren N

    The 14th International Workshop on Plant Membrane Biology　2007/06/26

  108. 高等植物における高親和型尿素トランスポーターの生理機能

    小島創一, Bohner A, 高橋秀樹, von Wirén N

    第2回トランスポーター研究会　2007/06

  109. Tissue-specific expression and influx studies of high-affinity urea transporter, AtDUR3 in Arabidopsis thaliana International-presentation

    Kojima S, Bohner A, von Wiren N

    2006 annual meeting of the German Society for Plant Nutrition　2006/09/28

  110. Tissue-specific expression and influx studies of high-affinity urea transporter, AtDUR3 in Arabidopsis thaliana International-presentation

    Kojima S, Bohner A, von Wiren N

    The 15th Congress in the Federation of European Societies in Plant Biology　2006/07/17

  111. Job sharing" among AMT-type ammonium transporters in Arabidopsis International-presentation

    Yuan L, Loque D, Gojon A, Takahashi H, Kojima S, von Wiren N

    The 15th Congress in the Federation of European Societies in Plant Biology　2006/07/17

  112. アラビドプシスの根におけるアンモニウムと尿素の輸送機構

    小島創一

    第13回 根圏制御技術 ネットワーク講演会　2005/12

  113. Contribution of the ammonium transporter AMT1;1 to diurnally regulated ammonium uptake in Arabidopsis roots. International-presentation

    Yuan L, Loqué D, Kojima S, von Wirén N

    The 15th International Plant Nutrition Colloquim　2005/09/14

  114. Identification of proteins that communicate with AtAMTs. International-presentation

    Kojima S, von Wirén N

    The 13th International Workshop on Plant Membrane Biology　2004/07/06

  115. Characterization of the physiological role of AtAMT1;2 in Arabidopsis thaliana. International-presentation

    von Wirén N, Rauch S, Yuan L, Loqué D, Gojon A, Ishiyama K, Takahashi H, Kojima S

    The 13th International Workshop on Plant Membrane Biology　2004/07/06

  116. Identification of proteins that communicate with AtAMTs. International-presentation

    Kojima S, von Wirén N

    The 7th International Symposium on Inorganic Nitrogen Assimilation in Plants: from the genome to the agro-ecosystem　2004/06/23

  117. The isoforms of glutamine synthetase are expressed and function in different manners depending on exogenous nitrogen status in Arabidopsis root

    Ishiyama K, Inoue E, Takahashi A, Obara M, Kataoka T, Kojima S, Yamaya T, Takahashi H

    RIKEN Plant Science Center Symp. Plant Science and the Environment　2003/11

  118. Differential expression of glutamine synthetase and glutamate synthase gene in Arabidopsis root International-presentation

    Ishiyama K, Inoue E, Takahashi A, Kojima S, von Wiren N, Yamaya T, Takahashi H

    14th Int. Conf. on Arabidopsis Research, The North American Arabidopsis Steering Committee　2003/06/20

  119. Identification of glutamine synthetase (GS) and glutamate synthase (GOGAT) responsible for assimilation of exogenous ammonium in Arabidopsis root.

    Ishiyama K, Inoue E, Takahashi A, Kojima S, Yamaya T, Takahashi H

    The 2003 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2003/03

  120. NADH-dependent glutamate synthase gene in Arabidopsis thaliana.

    Kojima S, Hayakawa T, Yamaya T

    The 2002 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2002/04

  121. Cis-elements that regulate vascular-bundle specific expression of NADH-GOGAT gene in rice.

    Kojima S, Hayakawa T, Yamaya T

    The 2002 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2002/03

  122. Biological functions of NADH dependent glutamate synthase in Arabidopsis thaliana.

    Kojima S, Maki H, Hayakawa T, Yamaya T

    The 2001 Annual Meeting and Simposia of the Japan Society for Bioscience, Biotechnology, and Agrobiochemistry　2001/03

  123. Investigation of cis element that regulates tissue-specific expression of gene for rice NADH-GOGAT

    Kojima S, Hayakawa T, Yamaya T

    The 2001 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　2001/03

  124. Analysis of the promoters for NADH dependent glutamate synthase and cytosolic glutamine synthethase in rice plant.

    Kojima S, Hanzawa S, Nozaki Y, Hayakawa T, Yamaya T

    The 2000 Annual Meeting and Simposia of the Japanese Society of Soil Science and Plant Nutrition　2000/04

  125. NADH-glutamate synthase, as a key enzyme in sink organs for re-utilization of glutamine remobilized from senescing organs in rice plants. International-presentation

    Kojima S, Ishiyama K, Hayakawa T, Yamaya T

    International Conference on Assimilate Transport and partitioning　1999/08/15

  126. Tissue specific and nitrogen inducible expression of rice NADH-GOGAT gene promoter in transgenic rice plants.

    Kojima S, Kimura M, Hayakawa T, Yamaya T

    The 1999 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　1999/03

  127. Molecular physiology of nitrogen recycling in rice plants. International-presentation

    Yamaya T, Kojima S, Obara M, Hayakawa T, Sato T

    The 12th Toyota Conference: Challenge of Plant and Agricultural Sciences to the Crisis of Biosphere on the Earth in the 21st Century　1998/11/25

  128. Expression of NADH-dependent glutamate synthase in rice roots. International-presentation

    Ishiyama K, Kojima S, Hayakawa T, Yamaya T

    5th International Symposium on Inorganic Nitrogen Assimilation　1998/07/13

  129. Analysis of NADH dependent glutamate synthase promoter in transgenic rice plants.

    Kojima S, Takahashi F, Goto S, Hayakawa T, Yamaya T

    The 1998 Annual Meeting and Simposia of the Japanese Society of Plant Physiologists　1998/05

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Research Projects 12

  1. The amino acid metabolism and transport mechanisms governing the translocation of nitrogen in rice are under variable nitrogen conditions.

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2022/04 - 2025/03

  2. Study on anorexia in heat-stressed broiler chickens: possible effects due to intestinal barrier dysfunction

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2020/04 - 2023/03

  3. ルーピンクラスター根の窒素欠乏環境における機能の解明

    Offer Organization: 公益財団法人タカノ農芸化学研究助成財団

    System: 研究助成金

    2021/04 - 2022/03

  4. Changes of GABA in the mutants of amino acid synthesis in rice

    Offer Organization: The Public Foundation of Elizabeth Arnold-Fuji

    Institution: Tohoku University

    2020/08 - 2021/07

  5. The reduction of acrylamide contamination in cooked rice with reverse genetics Competitive

    Offer Organization: THE TOYO SUISAN FOUNDATION

    System: Research grant

    Institution: Tohoku University

    2018/04 - 2019/03

  6. Seed storage protein in rice mutants for nitrogen metabolism Competitive

    Offer Organization: Tojuro Iijima Foundation for Food Science and Technology

    System: Research grant

    Institution: Tohoku University

    2016/04 - 2017/03

  7. The study of ammonium assimilation in Arabidopsis ecotypes

    Kojima Soichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04 - 2017/03

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    The biomass of the Arabidopsis ecotypes grown in ammonium medium was found to negatively correlate with the root length of the ecotypes grown in nitrogen deficient condition. This suggests the disadvantage of the developing root in excess ammonium. Arabidopsis expresses four GLN1 genes in a root. The reverse genetic study revealed the essential role of GLN1;2 in ammonium assimilation, and in the synthesis of glutamine which represses the expression of ammonium transporter.

  8. Nitrogen nutrient transport in rice root Competitive

    Offer Organization: Takano Life Science Research Foundation

    System: Takano Life Science Research Grant

    2014/05 - 2015/04

  9. Strategies for survival and growth of rice plants in response to nitrogen starved conditions

    YAMAYA Tomoyuki, KUSANO Miyako, HAYAKAWA Toshihiko, KOJIMA Soichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: Tohoku University

    2010/04 - 2015/03

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    Under nitrogen starved conditions, rice plants reduce their active tiller number in vegetative stage and their spikelet number in reproductive stage. However, molecular mechanisms in these responses are largely not known. We have carried out 1) NH4+-signal perception, 2) assimilation and remobilization of nitrogen, 3) metabolic balance between C and N, and 4) modeling of spikelet-ripening to understand molecular mechanisms of response. We obtained clear results, using reverse genetics approaches together with localization works, that glutamine synthetase (GS)1;2 was important in both the primary assimilation of NH4+ and outgrowth of axillary tiller buds, whereas GS1;1 is important in remobilization of N from senescing organs. NADH-glutamate synthase2 was tightly related to the determination of panicle number. These mutants showed metabolic disorder, indicating that N metabolism is important in normal growth and development of rice. Those results were published in several journals.

  10. Ammonium nutrient dependent root development in plant

    KOJIMA Soichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)

    Category: Grant-in-Aid for Young Scientists (A)

    Institution: Tohoku University

    2009 - 2011

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    Localized ammonium supply to Arabidopsis root increased root branching. The response was highly dependent on high-affinity ammonium transporter located at plasma membrane. This new knowledge may lead to improve nitrogen use efficiency in plant.

  11. Transport and use of urea in plant

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (Start-up)

    Category: Grant-in-Aid for Young Scientists (Start-up)

    Institution: Tohoku University

    2008 - 2009

  12. Transcriptional regulation of rice NADH dependent glutamate synthase

    2001 - 2002

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Social Activities 1

  1. 仙台向山高校インターンシップ

    2016/08/03 - 2016/08/05

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    仙台向山高校の学生数人を研究室へ受け入れして植物分子生物学の基本的な実験を実習した。

Academic Activities 1

  1. Peer review

    2008 - Present

    Activity type: Scientific advice/Review

Other 1

  1. website maintenance of laboratory

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    I have created laboratory website with Adobe Dream Weaver since 2009.