Details of the Researcher

PHOTO

Hayato Yokoi
Section
Graduate School of Agricultural Science
Job title
Professor
Degree

  • 博士（理学） (Nagoya University)

e-Rad No.
40569729

Committee Memberships 5

  • 日本動物学会　国際交流委員会

    2024/07 - Present

  • 日本動物学会 東北支部　支部委員

    2019/03 - Present

  • 日本水産学会　東北支部 支部連絡調整員

    2021/04 - 2022/03

  • 日本水産学会　東北支部 支部連絡調整員

    2016/04 - 2018/03

  • 日本水産学会　東北支部 編集担当委員

    2010/04 - 2012/03

Professional Memberships 4

  • The Zoological Society of Japan

  • The Japanese Society of Fisheries Science

  • The International Society of Developmental Biologists

  • Japanese Society of Developmental Biologists

Research Interests 4

  • Genomics

  • Evolution

  • Embryo manipulation

  • Metamorphosis in Fish

Research Areas 1

  • Life sciences / Aquaculture /

Awards 1

  1. Zebrafish Meeting Best Poster Award

    2004/08　6th International Conference on Zebrafish Development & Genetics　Poster presentation: Analysis of a medaka mutant headfish, defective in trunk and tail development.

Papers 49

  1. A genetic model of congenital intestinal atresia implicates Mypt1 in epithelial organisation. International-journal Peer-reviewed

    Daisuke Kobayashi, Akihiro Urasaki, Tetsuaki Kimura, Satoshi Ansai, Kazuhiko Matsuo, Hayato Yokoi, Shigeo Takashima, Tadao Kitagawa, Takahiro Kage, Takanori Narita, Tomoko Jindo, Masato Kinoshita, Kiyoshi Naruse, Yoshiro Nakajima, Masaki Shigeta, Shinichiro Sakaki, Satoshi Inoue, Rie Saba, Kei Yamada, Takahiko Yokoyama, Yuji Ishikawa, Kazuo Araki, Yumiko Saga, Hiroyuki Takeda, Kenta Yashiro

    Disease Models & Mechanisms　19　(2)　2026/02/01

    DOI： 10.1242/dmm.052605 　

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    Congenital intestinal atresia (IA) is a birth defect characterised by the absence or closure of part of the intestine. Although genetic factors are implicated, mechanistic understanding has been hindered by the lack of suitable animal models. Here, we describe a medaka (Oryzias latipes) mutant, generated by N-ethyl-N-nitrosourea (ENU) mutagenesis, that develops IA during embryogenesis. Positional cloning identified a nonsense mutation in mypt1, encoding myosin phosphatase target subunit 1. Mutant embryos exhibited ectopic accumulation of F-actin and phosphorylated myosin regulatory light chain (Mrlc) in the intestinal epithelium, consistent with disrupted actomyosin regulation. These cytoskeletal abnormalities were accompanied by epithelial disorganisation, without notable alterations in cell proliferation, motility or apoptosis. Inhibition of myh11a, encoding smooth muscle (SM) myosin heavy chain, ameliorated the IA phenotype, whereas blebbistatin treatment completely rescued the defect, suggesting a non-contractile role prior to SM maturation. Together, these findings demonstrate that mypt1 loss disrupts intestinal morphogenesis through actomyosin dysregulation. Given the recent clinical identification of IA associated with MYPT1 variants, this medaka model offers a valuable platform to investigate the developmental and molecular basis of MYPT1-associated IA in humans.

  2. Simple Transgene Overexpression using Scallop Hemocyte Culture Platform Enables Functional Genetic Research in Molluscs. International-journal Peer-reviewed

    Jeongwoong Yoon, Taro Tsuda, Enrico Bortoletto, Akari Sakaguchi, Mutsuko Kobayashi, Umberto Rosani, Hayato Yokoi, Makoto Osada, Paola Venier, Kazue Nagasawa

    Marine Biotechnology (New York, N.Y.)　27　(3)　94-94　2025/06/11

    DOI： 10.1007/s10126-025-10472-7 　

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    Despite being evolutionarily and commercially important, molluscs have been a traditionally challenging group to study, due to their difficulty in maintenance under lab conditions and the lack of a genetic toolkit. Previously, we showed that transgene expression can be attained in molluscan cells with reporter genes under a molluscan virus promoter sequence. Following up, we developed a simple, efficient and rapid transgene expression platform using primary hemocyte culture of Farrer's scallop Chlamys farreri, a marine bivalve mollusc. The protocol consists of two steps: collection and seeding of hemocytes and incubation for 1 to 4 days with DNA-reagent mixture. We evaluated seven transfection reagents for three bivalve species and found that X-tremeGENE 360 was highly efficient for DNA transfection, particularly for C. farreri. Subsequently, C. farreri hemocyte culture and transfection conditions were examined, such as culture medium, size and form of DNA, and the mixing ratio of DNA and transfection reagent. Using this protocol, we visualized the subcellular localization of four bivalve oncogenes, Cf-Mdm2-like, Cf-c-Myc-like, Cf-Mortalin-like, and Cf-Ras-like, tagged with EGFP. Our hemocyte platform provides an easy entry to study cellular and molecular biology of molluscs and can be readily adapted for advanced methods such as live imaging and DNA-protein interaction assays, making the study of molluscs more accessible to the scientific community.

  3. Vitellogenin uptake activity in the intestinal ducts of intraovarian embryos in a viviparous teleost Xenotoca eiseni International-journal Peer-reviewed

    Jumpei Nomura, Hayato Yokoi, Eiichi Hondo, Atsuo Iida

    Biochemical and Biophysical Research Communications　644　79-84　2023/01

    DOI： 10.1016/j.bbrc.2023.01.009 　

  4. Gene delivery available in molluscan cells by strong promoter discovered from bivalve-infectious virus. International-journal Peer-reviewed

    Jeongwoong Yoon, Wen-Bin Gu, Mizuki Konuma, Mutsuko Kobayashi, Hayato Yokoi, Makoto Osada, Kazue Nagasawa

    Proceedings of the National Academy of Sciences of the United States of America　119　(45)　e2209910119　2022/11/08

    DOI： 10.1073/pnas.2209910119 　

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    Understanding gene functions in marine invertebrates has been limited, largely due to the lack of suitable assay systems. Such a system requires investigative methods that are reproducible and can be quantitatively evaluated, such as a cell line, and a strong promoter that can drive high expression of a transgene. In this study, we established primary cell culture from a marine bivalve mollusc, Mizuhopecten yessoensis. Using scallop primary cells, we optimized electroporation conditions for transfection and carried out a luciferase-based promoter activity assay to identify strong promoter sequences that can drive expression of a gene of interest. We evaluated potential promoter sequences from genes of endogenous and exogenous origin and discovered a strong viral promoter derived from a bivalve-infectious virus, ostreid herpesvirus-1 (OsHV-1). This promoter, we termed OsHV-1 promoter, showed 24.7-fold and 16.1-fold higher activity than the cytomegalovirus immediate early (CMV IE) promoter and the endogenous EF1α promoter, the two most commonly used promoters in bivalves so far. Our GFP assays showed that the OsHV-1 promoter is active not only in scallop cells but also in HEK293 cells and zebrafish embryos. The OsHV-1 promoter practically enables functional analysis of marine molluscan genes, which can contribute to unveiling gene-regulatory networks underlying astonishing regeneration, adaptation, reproduction, and aging in marine invertebrates.

  5. Endocytosis-mediated vitellogenin absorption and lipid metabolism in the hindgut-derived placenta of the viviparous teleost Xenotoca eiseni International-journal Peer-reviewed

    Atsuo Iida, Jumpei Nomura, Junki Yoshida, Takayuki Suzuki, Hayato Yokoi, Eiichi Hondo

    Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids　1867　(9)　159183-159183　2022/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.bbalip.2022.159183 　

    ISSN： 1388-1981

  6. Myosin phosphatase target subunit 1governs integrity of the embryonic gut epithelium to circumvent atresia development in medaka,Oryzias latipes

    Daisuke Kobayashi, Kazuhiko Matsuo, Tetsuaki Kimura, Satoshi Ansai, Hayato Yokoi, Shigeo Takashima, Tadao Kitagawa, Takahiro Kage, Takanori Narita, Tomoko Jindo, Masato Kinoshita, Kiyoshi Naruse, Yoshiro Nakajima, Masaki Shigeta, Shinichiro Sakaki, Satoshi Inoue, Rie Saba, Kei Yamada, Takahiko Yokoyama, Yuji Ishikawa, Kazuo Araki, Yumiko Saga, Hiroyuki Takeda, Kenta Yashiro

    2021/12/11

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1101/2021.12.10.472183 　

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    Abstract Background Intestinal atresia (IA) is a congenital gut obstruction caused by the absence of gut opening. Genetic factors are assumed to be critical for the development of IA, in addition to accidental vascular insufficiency or mechanical strangulation. However, the molecular mechanism underlying IA remains poorly understood. Results In this study, to better understand such a mechanism, we isolated a mutant ofOryzias latipes(the Japanese rice fish known as medaka) generated by N-ethyl-N-nitrosourea mutagenesis, in which IA develops during embryogenesis. Positional cloning identified a nonsense mutation in themyosin phosphatase target subunit 1(mypt1) gene. Consistent with known Mypt1 function, the active form of myosin regulatory light chain (MRLC), which is essential for actomyosin contraction, and F-actin were ectopically accumulated in the intestinal epithelium of mutant embryos, whereas cell motility, proliferation and cell death were not substantially affected. Corresponding to the accumulation site of F-actin/active MRLC, the intestinal epithelium architecture was disordered. Importantly, blebbistatin, a non-muscle myosin inhibitor, attenuated the development of IA in the mutant. Conclusions Cytoskeletal contraction governed bymypt1regulates the integrity of the embryonic intestinal epithelium. This study provides new insight into our understanding of the mechanism of IA development in humans. Bullet Points Medakamypt1mutants display intestinal atresia. The level of phosphorylated myosin regulatory light chain was higher inmypt1mutant embryos than inwild-typeembryos. The levels of F-actin appeared elevated in the intestinal epithelium ofmypt1mutants. Blebbistatin, an inhibitor of non-muscle myosin II, rescued intestinal atresia inmypt1mutant embryos.

  7. Visualization of Sox10‐positive chromatoblasts by GFP fluorescence in flounder larvae and juveniles using electroporation Peer-reviewed

    Minato Miyake, Michiharu Sekine, Tohru Suzuki, Hayato Yokoi

    Journal of Experimental Zoology Part B: Molecular and Developmental Evolution　2021/04/26

    Publisher: Wiley

    DOI： 10.1002/jez.b.23045 　

    ISSN： 1552-5007

    eISSN： 1552-5015

  8. Delivery of exogenous proteins into eggs by injection into the mother's ovary (IMO) in zebrafish. International-journal Peer-reviewed

    Masaki Iwaizumi, Hayato Yokoi, Tohru Suzuki

    Fish Physiology and Biochemistry　47　(4)　849-855　2021/03/20

    DOI： 10.1007/s10695-021-00945-6 　

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    Genome editing has had profound effects on biological experimentation and can now be applied to many organisms, including non-conventional models. However, the introduction of genome editing components is time- and labor-consuming and sometimes requires special skills for microinjection. In this study, we developed a technique to deliver exogenous proteins into eggs by injection into the mother's ovary (IMO), which leads to the delivery of CRISPR/Cas9 into the eggs of oviparous animals, including fish. To test this technique, we examined whether exogenous proteins tagged with GFP or luciferase (Luc), and fluorescent-labeled RNP (Cas9 and sgRNA complex), can be delivered into eggs by IMO. When GFP-Luc or Cas9-Luc was delivered by IMO, their incorporation into fertilized eggs was confirmed by GFP fluorescence or luciferase activity; proteins were accumulated in the yolk. Cas9-RNP (targeting tyrosinase) was also incorporated into the eggs. However, genome editing of the target gene, tyrosinase, was not observed yet. This is presumably because the RNP delivered by IMO was packed in the yolk granules and did not reach into the embryonic nuclei. Thus, this report shows that exogenous molecules including Cas9-RNP were successfully delivered into fertilized eggs by IMO. Transferring the delivered RNP into nuclei will be critical for successful genome editing via the IMO delivery system.

  9. Development of the anterior lateral line system through local tissue-tissue interactions in the zebrafish head. International-journal Peer-reviewed

    Miki Iwasaki, Hayato Yokoi, Tohru Suzuki, Koichi Kawakami, Hironori Wada

    Developmental Dynamics : an official publication of the American Association of Anatomists　249　(12)　1440-1454　2020/12

    DOI： 10.1002/dvdy.225 　

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    BACKGROUND: The distribution of sensory organs is important for detecting environmental signals efficiently. The mechanosensory receptors of the lateral line system, neuromasts, are stereotypically distributed over the head and body surface of fish, although how neuromasts arise in these predetermined positions during development remains unclear. RESULTS: We investigated the development of the anterior lateral line (ALL) system in zebrafish head. The ALL neuromasts formed in the predetermined positions through proliferation and differentiation of (a) nonmigratory lateral line primordia, (b) migratory primordia, (c) interneuromast cells connecting preexisting neuromasts, and (d) budding primordia. We demonstrated that R-spondin2 (Rspo2), an activator of Wnt/β-catenin signaling, is required for the development of a particular set of neuromasts associated with hyomandibular cartilage. Further genetic analyses suggested that Rspo2, which emanates from the hyoid mesenchyme, acts on the adjacent neuromast progenitor cells to stimulate their proliferation through activating Wnt/β-catenin signaling. CONCLUSION: This study has revealed novel mechanisms for neuromast positioning through local tissue-tissue interactions, providing insights into the development and evolution of the vertebrate head.

  10. Plasmid delivery by electroporation into fish skeletal muscle for recombinant protein secretion and uptake by oocytes International-journal Peer-reviewed

    Masaki Iwaizumi, Hayato Yokoi, Tohru Suzuki

    Fish Physiology and Biochemistry　46　(3)　1121-1130　2020/06

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10695-020-00775-y 　

    ISSN： 0920-1742

    eISSN： 1573-5168

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    We examined the efficiency of electroporation for the delivery of plasmid into the skeletal muscle and also examined the subsequent secretion of recombinant protein into the circulation system, using zebrafish, Japanese flounder, and bubble-eye goldfish. The expression area of GFP fluorescence was markedly expanded by electroporation. Introduced plasmid was retained in the muscle cells and continued to express GFP for at least 180 days in zebrafish, indicating the long lifespan of plasmid DNA in the muscle cell. Luciferase and a fusion of growth hormone (GH) and luciferase were secreted into the blood from muscle electroporated with CMV:secNluc and CMV:GH-Luc plasmids, respectively, indicating that recombinant proteins such as peptide hormones can be supplied to the blood by plasmid electroporation into muscle. Interestingly, luciferase activity was detected from fertilized eggs laid by zebrafish females that had been electroporated with CMV:secNluc, indicating that maturing oocytes incorporated recombinant protein from the blood stream that had been secreted from the muscle. The plasmid electroporation system reported here also may work for the delivery of recombinant proteins, such as Cas9, into the oocytes. Since the GH-Luc fusion protein was detected in the lymph of the eye-sac of bubble-eye goldfish, this fish may be useful for the production of recombinant protein.

  11. Developmental regulatory system of ocular‐side‐specific asymmetric pigmentation in flounder: Critical role of retinoic acid signaling International-journal Peer-reviewed

    Qiran Chen, Kota Sato, Hayato Yokoi, Tohru Suzuki

    Journal of Experimental Zoology Part B: Molecular and Developmental Evolution　334　(3)　156-167　2020/05

    Publisher: Wiley

    DOI： 10.1002/jez.b.22934 　

    ISSN： 1552-5007

    eISSN： 1552-5015

  12. Mother-to-embryo vitellogenin transport in a viviparous teleost Xenotoca eiseni. International-journal Peer-reviewed

    Atsuo Iida, Hiroyuki N Arai, Yumiko Someya, Mayu Inokuchi, Takeshi A Onuma, Hayato Yokoi, Tohru Suzuki, Eiichi Hondo, Kaori Sano

    Proceedings of the National Academy of Sciences of the United States of America　116　(44)　22359-22365　2019/10/29

    DOI： 10.1073/pnas.1913012116 　

    ISSN： 0027-8424

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    Vitellogenin (Vtg), a yolk nutrient protein that is synthesized in the livers of female animals, and subsequently carried into the ovary, contributes to vitellogenesis in oviparous animals. Thus, Vtg levels are elevated during oogenesis. In contrast, Vtg proteins have been genetically lost in viviparous mammals, thus the yolk protein is not involved in their oogenesis and embryonic development. In this study, we identified Vtg protein in the livers of females during the gestation of the viviparous teleost, Xenotoca eiseni Although vitellogenesis is arrested during gestation, biochemical assays revealed that Vtg protein was present in ovarian tissues and lumen fluid. The Vtg protein was also detected in the trophotaeniae of the intraovarian embryo. Immunoelectron microscopy revealed that Vtg protein is absorbed into intracellular vesicles in the epithelial cells of the trophotaeniae. Furthermore, extraneous Vtg protein injected into the abdominal cavity of a pregnant female was subsequently detected in the trophotaeniae of the intraovarian embryo. Our data suggest that the yolk protein is one of the matrotrophic factors supplied from the mother to the intraovarian embryo during gestation in X. eiseni.

  13. Identification of Sox10-positive cells at the dorsal fin base of juvenile flounder that are correlated with blind-side skin ectopic pigmentation International-journal Peer-reviewed

    Mai Togawa, Yuna Endo, Nobuo Suzuki, Hayato Yokoi, Tohru Suzuki

    Journal of Experimental Zoology Part B: Molecular and Developmental Evolution　330　(8)　427-437　2018/12

    DOI： 10.1002/jez.b.22842 　

    ISSN： 1552-5007

    eISSN： 1552-5015

  14. Expression profiles of RA synthases and catabolic enzymes in newly hatched and metamorphosing larvae of Japanese flounder, Paralichthys olivaceus International-journal Peer-reviewed

    Qiran Chen, Hayato Yokoi, Tohru Suzuki

    General and Comparative Endocrinology　269　60-67　2018/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.ygcen.2018.08.015 　

    ISSN： 0016-6480

  15. External Asymmetry and Pectoral Fin Loss in the Bamboo Sole (Heteromycteris japonica): Small-Sized Sole with Potential as a Pleuronectiformes Experimental Model International-journal Peer-reviewed

    Qiran Chen, Masako Takagi, Makoto Mogi, Miki Kikuchi, Yudai Saito, Shunya Nakamura, Hayato Yokoi, Tadahisa Seikai, Susumu Uji, Tohru Suzuki

    Zoological Science　34　(5)　377-385　2017/10

    DOI： 10.2108/zs170021 　

    ISSN： 0289-0003

  16. Analyses of the cellular clock gene expression in peripheral tissue, caudal fin, in the Japanese flounder, Paralichthys olivaceus International-journal Peer-reviewed

    Makoto Mogi, Hayato Yokoi, Tohru Suzuki

    General and Comparative Endocrinology　248　97-105　2017/07

    DOI： 10.1016/j.ygcen.2017.02.009 　

    ISSN： 0016-6480

    eISSN： 1095-6840

  17. Excess Retinoic Acid Induces Fusion of Centra by Degenerating Intervertebral Ligament Cells in Japanese flounder, Paralichthys olivaceus International-journal Peer-reviewed

    Xiaoming Wu, Qiran Chen, Youhei Washio, Hayato Yokoi, Tohru Suzuki

    Journal of Experimental Zoology Part B: Molecular and Developmental Evolution　326　(8)　464-473　2016/12

    DOI： 10.1002/jez.b.22717 　

    ISSN： 1552-5007

    eISSN： 1552-5015

  18. Role of notochord cells and sclerotome-derived cells in vertebral column development in fugu, Takifugu rubripes: histological and gene expression analyses International-journal Peer-reviewed

    Takamasa Kaneko, Khalid Freeha, Xiaoming Wu, Makoto Mogi, Susumu Uji, Hayato Yokoi, Tohru Suzuki

    Cell and Tissue Research　366　(1)　37-49　2016/10

    DOI： 10.1007/s00441-016-2404-z 　

    ISSN： 0302-766X

    eISSN： 1432-0878

  19. Diethylnitrosamine-induced expression of germline-specific genes and pluripotency factors, including vasa and oct4, in medaka somatic cells International-journal Peer-reviewed

    Jialing Shen, Shinpei Yokota, Hayato Yokoi, Tohru Suzuki

    Biochemical and Biophysical Research Communications　478　(2)　858-863　2016/09

    DOI： 10.1016/j.bbrc.2016.08.039 　

    ISSN： 0006-291X

    eISSN： 1090-2104

  20. Establishment of oct4:egfp transgenic and oct4:egfp/beta-actin:DsRed double transgenic medaka lines International-journal Peer-reviewed

    Shinpei Yokota, Rinta Matsuno, Hiroyuki Kato, Hisashi Hashimoto, Masato Kinoshita, Hayato Yokoi, Tohru Suzuki

    In Vitro Cellular & Developmental Biology-Animal　52　(6)　646-653　2016/06

    DOI： 10.1007/s11626-016-0020-6 　

    ISSN： 1071-2690

    eISSN： 1543-706X

  21. Transcriptome analysis of vertebral bone in the flounder, Paralichthys olivaceus (Teleostei, Pleuronectiformes), using Illumina sequencing International-journal Peer-reviewed

    Harumi Ibaraki, Xiaoming Wu, Susumu Uji, Hayato Yokoi, Yoshifurni Sakai, Tohru Suzuki

    Marine Genomics　24　269-276　2015/12

    DOI： 10.1016/j.margen.2015.09.009 　

    ISSN： 1874-7787

    eISSN： 1876-7478

  22. Early development of circadian rhythmicity in the suprachiamatic nuclei and pineal gland of teleost, flounder (Paralichthys olivaeus), embryos International-journal Peer-reviewed

    Makoto Mogi, Susumu Uji, Hayato Yokoi, Tohru Suzuki

    Development Growth & Differentiation　57　(6)　444-452　2015/08

    DOI： 10.1111/dgd.12222 　

    ISSN： 0012-1592

    eISSN： 1440-169X

  23. Establishment of oct4:gfp transgenic zebrafish line for monitoring cellular multipotency by GFP fluorescence International-journal Peer-reviewed

    Hiroyuki Kato, Kota Abe, Shinpei Yokota, Rinta Matsuno, Tsuyoshi Mikekado, Hayato Yokoi, Tohru Suzuki

    In Vitro Cellular & Developmental Biology-Animal　51　(1)　42-49　2015/01

    DOI： 10.1007/s11626-014-9805-7 　

    ISSN： 1071-2690

    eISSN： 1543-706X

  24. Exposure to oxidative by-products during metamorphosis affects pigmentation patterns in flounder, Paralichthys olivaceus International-journal Peer-reviewed

    Youhei Washio, Yuichiro Fujinami, Daisuke Shimizu, Hayato Yokoi, Tohru Suzuki

    Aquaculture　435　318-327　2015/01

    DOI： 10.1016/j.aquaculture.2014.10.015 　

    ISSN： 0044-8486

    eISSN： 1873-5622

  25. No Evidence for AID/MBD4-Coupled DNA Demethylation in Zebrafish Embryos International-journal Peer-reviewed

    Nobuyoshi Shimoda, Kentaro Hirose, Reiya Kaneto, Toshiaki Izawa, Hayato Yokoi, Naohiro Hashimoto, Yutaka Kikuchi

    PLOS ONE　9　(12)　e114816-e114816　2014/12

    DOI： 10.1371/journal.pone.0114816 　

    ISSN： 1932-6203

  26. TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development International-journal Peer-reviewed

    Yoshiaki Tatsumi, Moe Takeda, Masaru Matsuda, Tohru Suzuki, Hayato Yokoi

    FEBS Letters　588　(24)　4543-4550　2014/12

    DOI： 10.1016/j.febslet.2014.10.015 　

    ISSN： 0014-5793

    eISSN： 1873-3468

  27. Decrease in cytosine methylation at CpG island shores and increase in DNA fragmentation during zebrafish aging International-journal Peer-reviewed

    Nobuyoshi Shimoda, Toshiaki Izawa, Akio Yoshizawa, Hayoto Yokoi, Yutaka Kikuchi, Naohiro Hashimoto

    AGE　36　(1)　103-115　2014/02

    DOI： 10.1007/s11357-013-9548-5 　

    ISSN： 0161-9152

    eISSN： 1574-4647

  28. Ocular-Side Lateralization of Adult-Type Chromatophore Precursors: Development of Pigment Asymmetry in Metamorphosing Flounder Larvae International-journal Peer-reviewed

    Youhei Washio, Masato Aritaki, Yuichiro Fujinami, Daisuke Shimizu, Hayato Yokoi, Tohru Suzuki

    Journal of Experimental Zoology Part B: Molecular and Developmental Evolution　320B　(3)　151-165　2013/05

    DOI： 10.1002/jez.b.22491 　

    ISSN： 1552-5007

    eISSN： 1552-5015

  29. Modular development of the teleost trunk along the dorsoventral axis and zic1/zic4 as selector genes in the dorsal module International-journal Peer-reviewed

    Toru Kawanishi, Takuya Kaneko, Yuuta Moriyama, Masato Kinoshita, Hayato Yokoi, Tohru Suzuki, Atsuko Shimada, Hiroyuki Takeda

    Development　140　(7)　1486-1496　2013/04

    DOI： 10.1242/dev.088567 　

    ISSN： 0950-1991

    eISSN： 1477-9129

  30. Circadian pacemaker in the suprachiasmatic nuclei of teleost fish revealed by rhythmic period2 expression International-journal Peer-reviewed

    Nanako Watanabe, Kae Itoh, Makoto Mogi, Yuichiro Fujinami, Daisuke Shimizu, Hiroshi Hashimoto, Susumu Uji, Hayato Yokoi, Tohru Suzuki

    General and Comparative Endocrinology　178　(2)　400-407　2012/09

    DOI： 10.1016/j.ygcen.2012.06.012 　

    ISSN： 0016-6480

  31. Continuous illumination through larval development suppresses dopamine synthesis in the suprachiasmatic nucleus, causing activation of alpha-MSH synthesis in the pituitary and abnormal metamorphic skin pigmentation in flounder International-journal Peer-reviewed

    Kae Itoh, Youhei Washio, Yuichiro Fujinami, Daisuke Shimizu, Susumu Uji, Hayato Yokoi, Tohru Suzuki

    General and Comparative Endocrinology　176　(2)　215-221　2012/04

    DOI： 10.1016/j.ygcen.2012.01.017 　

    ISSN： 0016-6480

    eISSN： 1095-6840

  32. Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence International-journal Peer-reviewed

    Yohei Matsuda, Yoshie Ito, Hisashi Hashimoto, Hayato Yokoi, Tohru Suzuki

    Reproductive Biology and Endocrinology　9　45　2011/04

    DOI： 10.1186/1477-7827-9-45 　

    ISSN： 1477-7827

  33. Evolution of Developmental Regulation in the Vertebrate FgfD Subfamily International-journal Peer-reviewed

    Richard Jovelin, Yi-Lin Yan, Xinjun He, Julian Catchen, Angel Amores, Cristian Canestro, Hayato Yokoi, John H. Postlethwait

    Journal of Experimental Zoology Part B: Molecular and Developmental Evolution　314B　(1)　33-56　2010/01

    DOI： 10.1002/jez.b.21307 　

    ISSN： 1552-5007

  34. Characterization of Teleost Mdga1 Using a Gene-Trap Approach in Medaka (Oryzias latipes) International-journal Peer-reviewed

    Shinya Sano, Shigeo Takashima, Hitomi Niwa, Hayato Yokoi, Atsuko Shimada, Alexander Arenz, Joachim Wittbrodt, Hiroyuki Takeda

    Genesis　47　(8)　505-513　2009/08

    DOI： 10.1002/dvg.20528 　

    ISSN： 1526-954X

  35. Consequences of Lineage-Specific Gene Loss on Functional Evolution of Surviving Paralogs: ALDH1A and Retinoic Acid Signaling in Vertebrate Genomes International-journal Peer-reviewed

    Cristian Canestro, Julian M. Catchen, Adriana Rodriguez-Mari, Hayato Yokoi, John H. Postlethwait

    PLOS Genetics　5　(5)　e1000496-e1000496　2009/05

    DOI： 10.1371/journal.pgen.1000496 　

    ISSN： 1553-7404

  36. Expression profiling of zebrafish sox9 mutants reveals that Sox9 is required for retinal differentiation International-journal Peer-reviewed

    Hayato Yokoi, Yi-Lin Yan, Michael R. Miller, Ruth A. BreMiller, Julian M. Catchen, Eric A. Johnson, John H. Postlethwait

    Developmental Biology　329　(1)　1-15　2009/05

    DOI： 10.1016/j.ydbio.2009.01.002 　

    ISSN： 0012-1606

  37. Mullerian inhibiting substance is required for germ cell proliferation during early gonadal differentiation in medaka (Oryzias latipes) International-journal Peer-reviewed

    Eri Shiraishi, Norifumi Yoshinaga, Takeshi Miura, Hayato Yokoi, Yuko Wakamatsu, Shin-Ichi Abe, Takeshi Kitano

    Endocrinology　149　(4)　1813-1819　2008/04

    DOI： 10.1210/en.2007-1535 　

    ISSN： 0013-7227

  38. Maternal-zygotic medaka mutants for fgfr1 reveal its essential role in the migration of the axial mesoderm but not the lateral mesoderm International-journal Peer-reviewed

    Atsuko Shimada, Mina Yabusaki, Hitomi Niwa, Hayato Yokoi, Kohei Hatta, Daisuke Kobayashi, Hiroyuki Takeda

    Development　135　(2)　281-290　2008/01

    DOI： 10.1242/dev.011494 　

    ISSN： 0950-1991

  39. Evolutionary developmental biology and genomics International-journal Invited Peer-reviewed

    Cristian Cañestro, Hayato Yokoi, John H. Postlethwait

    Nature Reviews Genetics　8　(12)　932-942　2007/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/nrg2226 　

    ISSN： 1471-0056

    eISSN： 1471-0064

  40. Phenotypic analysis of a novel chordin mutant in medaka International-journal Peer-reviewed

    Shigeo Takashima, Atsuko Shimada, Daisuke Kobayashi, Hayato Yokoi, Takanori Narita, Tomoko Jindo, Takahiro Kage, Tadao Kitagawa, Tetsuaki Kimura, Koshin Sekimizu, Akimitsu Miyake, Davin H. E. Setiamarga, Ryohei Murakami, Sachiko Tsuda, Shinya Ooki, Ken Kakihara, Motoki Hojo, Kiyoshi Naruse, Hiroshi Mitani, Akihiro Shima, Yuji Ishikawa, Kazuo Araki, Yumiko Saga, Hiroyuki Takeda

    Developmental Dynamics　236　(8)　2298-2310　2007/08

    DOI： 10.1002/dvdy.21245 　

    ISSN： 1058-8388

  41. Right-elevated expression of charon is regulated by fluid flow in medaka Kupffer's vesicle International-journal Peer-reviewed

    Motoki Hojo, Shigeo Takashima, Daisuke Kobayashi, Akira Sumeragi, Atsuko Shimada, Tatsuya Tsukahara, Hayato Yokoi, Takanori Narita, Tomoko Jindo, Takahiro Kage, Tadao Kitagawa, Tetsuaki Kimura, Koshin Sekimizu, Akimitsu Miyake, Davin Setiamarga, Ryohei Murakami, Sachiko Tsuda, Shinya Ooki, Ken Kakihara, Kiyoshi Naruse, Hiroyuki Takeda

    Development Growth & Differentiation　49　(5)　395-405　2007/06

    DOI： 10.1111/j.1440-169x.2007.00937.x 　

    ISSN： 0012-1592

    eISSN： 1440-169X

  42. Mutant analyses reveal different functions of fgfr1 in medaka and zebrafish despite conserved ligand-receptor relationships Peer-reviewed

    Hayato Yokoi, Atsuko Shimada, Matthias Carl, Shigeo Takashima, Daisuke Kobayashi, Takanori Narita, Tomoko Jindo, Tetsuaki Kimura, Tadao Kitagawa, Takahiro Kage, Atsushi Sawada, Kiyoshi Naruse, Shuichi Asakawa, Nobuyoshi Shimizu, Hiroshi Mitani, Akihiro Shima, Makiko Tsutsumi, Hiroshi Hori, Joachim Wittbrodt, Yumiko Saga, Yuji Ishikawa, Kazuo Araki, Hiroyuki Takeda

    Developmental Biology　304　(1)　326-337　2007/04

    DOI： 10.1016/j.ydbio.2006.12.043 　

    ISSN： 0012-1606

  43. Identification and lineage tracing of two populations of somatic gonadal precursors in medaka embryos International-journal Peer-reviewed

    Shuhei Nakamura, Daisuke Kobayashi, Yumiko Aoki, Hayato Yokoi, Youko Ebe, Joachim Wittbrodt, Minoru Tanaka

    Developmental Biology　295　(2)　678-688　2006/07

    DOI： 10.1016/j.ydbio.2006.03.052 　

    ISSN： 0012-1606

  44. Identification of two teleost homologs of the Drosophila sex determination factor, transformer-2 in medaka (Oryzias latipes) International-journal Peer-reviewed

    E Shiraishi, H Imazato, T Yamamoto, H Yokoi, S Abe, T Kitano

    Mechanisms of Development　121　(7-8)　991-996　2004/07

    DOI： 10.1016/j.mod.2004.04.013 　

    ISSN： 0925-4773

  45. Possible roles of zic1 and zic4, identified within the medaka Double anal fin (Da) locus, in dorsoventral patterning of the trunk-tail region (related to phenotypes of the Da mutant) International-journal Peer-reviewed

    M Ohtsuka, N Kikuchi, H Yokoi, M Kinoshita, Y Wakamatsu, K Ozato, H Takeda, H Inoko, M Kimura

    Mechanisms of Development　121　(7-8)　873-882　2004/07

    DOI： 10.1016/j.mod.2004.04.006 　

    ISSN： 0925-4773

  46. A mutation in the gene for delta-aminolevulinic acid dehydratase (ALAD) causes hypochromic anemia in the medaka, Oryzias latipes International-journal Peer-reviewed

    D Sakamoto, H Kudo, K Inohaya, H Yokoi, T Narita, K Naruse, H Mitani, K Araki, A Shima, Y Ishikawa, Y Imai, A Kudo

    Mechanisms of Development　121　(7-8)　747-752　2004/07

    DOI： 10.1016/j.mod.2004.03.030 　

    ISSN： 0925-4773

  47. Cloning and embryonic expression of six wnt genes in the medaka (Oryzias latipes) with special reference to expression of wnt5a in the pectoral fin buds International-journal Peer-reviewed

    H Yokoi, A Nishimatsu, K Ozato, K Yoda

    Development Growth & Differentiation　45　(1)　51-61　2003/02

    DOI： 10.1046/j.1440-169X.2003.00674.x 　

    ISSN： 0012-1592

  48. sox9 in a teleost fish, medaka (Oryzias latipes): Evidence for diversified function of Sox9 in gonad differentiation International-journal Peer-reviewed

    H Yokoi, T Kobayashi, M Tanaka, Y Nagahama, Y Wakamatsu, H Takeda, K Araki, KI Morohashi, K Ozato

    Molecular Reproduction and Development　63　(1)　5-16　2002/09

    DOI： 10.1002/mrd.10169 　

    ISSN： 1040-452X

  49. Injection of DNA into the medaka oocyte nucleus. International-journal Peer-reviewed

    Yokoi H, Ozato K

    Fish Biol. J. MEDAKA　7　53-58　1995/12

    Publisher: Nagoya University

    DOI： 10.18999/fisbjm.7.53 　

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Misc. 6

  1. Zebrafish as a Model for the Study of Gut Microbiome Invited Peer-reviewed

    IKEDA-OHTSUBO Wakako, YOKOI Hayato

    Journal of Intestinal Microbiology　38　(4)　191-201　2024/10

    Publisher: The Intestinal Microbiology Society

    DOI： 10.11209/jim.38.191 　

    ISSN： 1343-0882

    eISSN： 1349-8363

    More details Close

    Zebrafish as a vertebrate model have contributed to elucidating the functions of important factors involved in development, metabolism, and various diseases that are shared among human and animals. Zebrafish have also attracted attention as a gut microbiota model and have been studied for host-microbe interaction using diverse approaches, which involve host immune, metabolic, and behavioral responses to specific microbes. Using zebrafish as disease models for diabetes and pathogen infection models has been especially valuable for the elucidation of important factors as candidate targets for pharmaceutical development. Zebrafish offer unique advantages over mammalian models as a gut microbiota model because their transparency enables live imaging of individual microbes and immune cells within the host. Rearing zebrafish and setting up experiments for microbiome research entail consideration of aquatic-specific factors that may significantly impact the microbiota. Although fish are not considered as experimental animals in Japan, the handling of fish with ethical consideration is required, due to the international trend toward the awareness of animal welfare including fish. In this review, we provide an overview of zebrafish as an experimental animal model and summarize recent important studies using zebrafish as a host of microbes. We then discuss perspectives on zebrafish research as an alternative animal model and international ethical differences in handling fish, and review the applicability and prospect of fish models for microbiome research.

  2. 腹腔内注射によりゲノム編集する新技術の開発に向けて －多様な養殖魚への応用を目指して－ Invited

    鈴木徹, 岩泉雅樹, 横井勇人

    アグリバイオ　2　(4)　380-381　2018/04

    Publisher: 北隆館

  3. Conservation and diversification of gene function: examples from Fgf receptor

    Hayato Yokoi

    Cell Technology　31　(4)　414-415　2012/03

    Publisher: Gakken Medical Shujunsha

  4. Function and diversification of the FGF receptor mediated signaling

    Yokoi H, Takeda H

    Cell Technology　24　(10)　1055-1059　2005/09/22

  5. PRODUCTION OF MATERNAL-ZYGOTIC MUTANT MEDAKA BY PGC TRANSPLANTATION INTO INTERSPECIFIC HYBRID(Developmental Biology,Abstracts of papers presented at the 76^<th> Annual Meeting of the Zoological Society of Japan) :

    Shimada Atsuko, Yokoi Hayato, Kanamori Akira, Hori Hiroshi, Kinoshita Masato, Takeda Hiroyuki

    Zoological science　22　(12)　1452-1452　2005

    Publisher: Zoological Society of Japan

    ISSN： 0289-0003

  6. Heritable malformations induced by X-irradiation and ENU in the medaka :

    YASUDA Takako, YOKOI Hayato, INOHAYA Keiji, SAKAMOTO Daigo, ICHIKAWA Kumi, OTA Munehiro, NARITA Takanori, AOKI Kazuko, SASANUMA Motoe, MATSUMOTO Atsuko, ISHIKAWA Yuji

    Journal of radiation research　42　(4)　518-518　2001

    Publisher: the Japan Radiation Research Society

    ISSN： 0449-3060

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Books and Other Publications 6

  1. What Controls Sex Development in Fish?

    Japanese version by Hayato Yokoi, Satoshi Ansai (Original English version written by Sophia Breslin, John H. Postlethwait, Thomas Desvignes)

    The Science and Comics Initiative, University of Oregon　2024

  2. 光が彩るヒラメ・カレイ類養殖 : 生命科学から応用まで = Flatfish aquaculture innovation with light : from life sciences to applications

    横井勇人, 鈴木徹 (編: 水澤寛太, 中村修) 日本水産学会

    恒星社厚生閣　2022/10

    ISBN: 9784769916871

  3. ゼブラフィッシュ実験ガイド

    横井勇人 (編: 平田普三)

    朝倉書店　2020/11

    ISBN: 9784254171730

  4. Whole genome duplication and diversification of gene function in teleosts: lessons from Fgfr1, Sox9 and Aldh genes. In "Advances in Comparative Endocrinology, vol IX"

    Yokoi H, Chen Q, Cañestro C, Postlethwait JH, Suzuki T

    Universida de Vigo　2017/12

    ISBN: 9788481587586

  5. 学生実験テキスト 東北大学農学部生物生産科学科海洋生物科学系

    横井勇人, 鈴木徹

    2013/03/22

  6. Genome duplication and subfunction partitioning: Sox9 in medaka and other vertebrates. In “Medaka: Model for Organogenesis, Human Disease and Evolution.”

    Yokoi H, Postlethwait JH

    Springer　2011/06/06

    ISBN: 9784431926900

    More details Close

    DOI 10.1007/978-4-431-92691-7_21

Show all Show first 5

Presentations 205

  1. ヒラメ変態の実験モデルとしてのメダカ

    横井勇人

    NBRPメダカユーザーミーティング2026　2026/03/01

  2. 色素胞分化に関与するgch遺伝子群の魚類特異的な進化と機能の解析

    原凜, 木野創太, 横井勇人

    日本分子生物学会 第48回年会　2025/12/03

  3. ヒラメの無眼側黒化変異体：ゲノム・遺伝学的解析と候補遺伝子の検討

    横井勇人, 安齋賢, 田川正朋

    日本動物学会 第96回 名古屋大会　2025/09/05

  4. ヒラメの左右非対称化と眼の移動のメカニズム

    横井勇人

    3Questions - 東北地区編 -　2025/08/25

  5. ヒラメの左右非対称な体色形成に関わるgch遺伝子群の機能解析

    原凛, 木野創太, 横井勇人

    2025年度 日本動物学会 東北支部大会　2025/07/27

  6. Gch genes in fish: two in medaka and zebrafish, and five in Japanese flounder International-presentation

    Rin Hara, Sota Kino, Hayato Yokoi

    Joint Annual Meeting of JSCB 77th & JSDB 58th　2025/07/16

  7. Analysis of the intestinal atresia mutant of medaka (Oryzias latipes)

    Kobayashi D, Urasaki A, Kimura T, Ansai S, Yokoi H, Takashima S, Kitagawa T, Kage T, Narita T, Jindo T, Kinoshita M, Naruse K, Ishikawa Y, Araki K, Saga Y, Takeda H, Yashiro K

    The 57th Annual Meeting of the Japanese Society of Developmental Biologists　2024/06/20

  8. 異体類のユニークな変態を理解したい Invited

    横井勇人

    ミニシンポジウム 東北地方の動物学の現状と将来　2024/02/17

  9. Genomic and genetic analysis of a flounder mutant defective in left-right asymmetric pigmentation found in an aquaculture hatchery

    Yokoi H, Ansai S, Tagawa M

    The 56th Annual Meeting of the Japanese Society of Developmental Biologists. Sendai International Center　2023/07/23

  10. ササウシノシタHeteromycteris japonica の参照ゲノム配列決定

    安齋賢, 横井勇人, 鈴木徹, 宇治督

    日本水産学会春季大会　2023/03/30

  11. ヒラメの無眼側黒化変異体の原因遺伝子の探索

    横井勇人, 安齋賢, 田川正朋

    日本水産学会春季大会　2023/03/30

  12. 海産軟体動物由来の初代細胞培養の樹立と二枚貝感染ウイルスプロモーターを用いた遺伝子導入系の確立

    Jeongwoong YOON, Wen-Bin GU, 小沼瑞, 小林睦子, 横井勇人, 尾定誠, 長澤一衛

    第46回日本比較内分泌学会大会及びシンポジウム　2022/10/29

  13. Diverse viral immediate-early genes for genetic engineering, Application in Mollusca

    Jeongwoong Yoon, Wen-Bin Gu, Mizuki Konuma, Mutsuko Kobayashi, Hayato Yokoi, Makoto Osada, Kazue Nagasawa

    Tohoku University – OIST 3rd Joint Workshop on Biodiversity: From Genes and Species to Ecosystem Services and Resilience　2022/10/24

  14. Flatfish metamorphosis: molecular basis underlying unique morphogenesis leading to asymmetric body plan Invited

    Hayato Yokoi, Qiran Chen, Tohru Suzuki

    Tohoku University – OIST 3rd Joint Workshop on Biodiversity: From Genes and Species to Ecosystem Services and Resilience　2022/10/25

  15. 非モデル生物ヒラメにおける外来遺伝子導入法の検討:メス親魚卵巣への注入法および筋肉組織へのエレクトロポレーション法

    安藤日向, 関根通陽, 岩泉雅樹, 鈴木徹, 横井勇人

    第7回ユニーク会　2022/09/20

  16. 水産軟体動物細胞で機能するウイルスプロモーターの発見

    Jeongwoong YOON, Wen-Bin GU, 小沼瑞, 小林睦子, 横井勇人, 尾定誠, 長澤一衛

    令和4年度日本水産学会秋季大会　2022/09/05

  17. ヒラメの色素胞分化に関与する 5 つの gch 遺伝子の比較解析

    木野創太, 鈴木徹, 横井勇人

    日本水産学会春季大会　2022/03/28

  18. Transcriptome approach to understand flatfish metamorphosis Invited

    Hayato Yokoi

    e-Asia sponsorship workshop: Development and Effective Utilization of Genetic Marker in Non-Experimental Fishes　2022/03/24

  19. ヒラメの変態とその分子メカニズムを理解したい Invited

    横井勇人, 鈴木徹

    2021年日本ベントス学会・日本プランクトン学会合同大会　2021/09/17

  20. Toward functional analysis in non-model organism, flounder

    Michiharu Sekine, Masaki Iwaizumi, Minato Miyake, Tohru Suzuki, Hayato Yokoi

    2021/06/18

  21. Retinoic acid is the key regulator of asymmetric pigmentation in the Japanese flounder larvae

    Qiran Chen, Kota Sato, Hayato Yokoi, Tohru Suzuki

    2021/03/27

  22. エレクトロポレーション法によるヒラメ筋肉への遺伝子導入の条件検討と応用

    関根通陽, 鈴木徹, 横井勇人

    日本水産学会春季大会　2021/03/27

  23. Retinoic acid plays critical role for flounder asymmetrical pigmentation

    Qiran Chen, Hayato Yokoi, Tohru Suzuki

    2020/12/06

  24. エレクトロポレーション法によるヒラメ筋肉への遺伝子導入の検討

    関根通陽, 鈴木徹, 横井勇人

    日本動物学会 2020年度東北支部大会　2020/12/06

  25. Localization of glucocorticoid receptor Nr3c1 in Japanese flounder (Paralichthys olivaceus)

    Naomi Hadisumarto, Makine Tsuneta, Hayato Yokoi, Tohru Suzuki

    2020/10/10

  26. Analysis of genes involved in xanthophore differentiation using GFP knock-in medaka

    2020/09/04

  27. GFP-knock-in medaka revealed the involvement of dhfr-like and hdd in the xanthophore lineage pigment cell differentiation

    Shunya Nakamura, Satoshi Ansai, Kiyoshi Naruse, Tohru Suzuki, Hayato Yokoi

    53rd Annual Meeting of the Japanese Society of Developmental Biologists (JSDB) Co-sponsored by Asia-Pacific Developmental Biology Network (APDBN)　2020/05/20

  28. 魚類の色素胞分化に関与する dhfr-like および hdd の解析

    中村峻也, 安齋賢, 成瀬清, 鈴木徹, 横井勇人

    日本水産学会春季大会　2020/03/27

  29. Immunohistochemical localization of glucocorticoid receptor (GcR; Nr3c1) in Japanese flounder larvae and juveniles

    2020/03/29

  30. エレクトロポーレーションを用いたヒラメ稚魚内の Sox10 陽性色素前駆細胞の in vivo での可視化

    三宅みなと, 鈴木徹, 横井勇人

    日本水産学会春季大会　2020/03/27

  31. ササウシノシタ仔魚における Sox10 陽性 - 色素前駆細胞の分布とWntアンタゴニストの影響

    佐藤航大, 宇治督, 横井勇人, 鈴木徹

    日本水産学会春季大会　2020/03/27

  32. Critical role of RA signaling during the asymmetric pigmentation of metamorphosing Japanese flounder

    2020/03/27

  33. 雌親魚の腹腔内注射による卵母細胞へのタンパク質の導入

    岩泉雅樹, 横井勇人, 宇治督, 鈴木徹

    日本水産学会春季大会　2020/03/27

  34. Role of gene body methylation revealed by a viable zebrafish DNA methyltransferase mutant

    Norimasa Iwanami, Hayato Yokoi, Shintaro Akiyama, Daichi Shigemizu, Natsuki Araki, Nao Ninomiya, Masaru Matsuda, Tohru Suzuki, Nobuyoshi Shimoda

    42nd Annual Meeting of the Molecular Biology Society of Japan　2019/12/04

  35. Gene expression profile change during larval development and metamorphosis in bamboo sole revealed by RNA-seq

    Yudai Saito, Hayato Yokoi, Susumu Uji, Tohru Suzuki

    90th Annual Meeting of the Zoological Society of Japan　2019/09/12

  36. Characterization of medaka dhfr-like and hdd, genes involved in pigment cell differentiation International-presentation

    Hayato Yokoi, Shunya Nakamura, Satoshi Ansai, Kiyoshi Naruse, Tohru Suzuki

    25th Japanese Medaka and Zebrafish Meeting　2019/09/04

  37. Immunohistochemical localization of glucocorticoid receptor (Nr3c1) in the brain of Japanese flounder (Paralichthys olivaceus) larva and juvenile International-presentation

    Naomi Hadisumarto, Makine Tsuneta, Hayato Yokoi, Tohru Suzuki

    25th Japanese Medaka and Zebrafish Meeting　2019/09/04

  38. Jaw cartilage regulates proliferation of the lateral line sensory organs through Wnt signaling in zebrafish International-presentation

    Miki Iwasaki, Hayato Yokoi, Tohru Suzuki, Koichi Kawakami, Hironori Wada

    25th Japanese Medaka and Zebrafish Meeting　2019/09/04

  39. De novo methylation of zebrafish angptl1b gene body by Dnmt3bb.2 secures the expression level and integrity of the transcript International-presentation

    Norimasa Iwanami, Hayato Yokoi, Shintaro Akiyama, Daichi Shigemizu, Natsuki Araki, Nao Ninomiya, Masaru Matsuda, Tohru Suzuki, Nobuyoshi Shimoda

    25th Japanese Medaka and Zebrafish Meeting　2019/09/04

  40. Development of efficient delivery method for genome editing in aquaculture fish

    Masaki Iwaizumi, Hayato Yokoi, Tohru Suzuki

    4th Annual Meeting of the Japanese Society for Genome Editing　2019/06/03

  41. dhfr and hdd, genes involved in pigment cell differentiation isolated by transcriptome analysis of flounder metamorphosis International-presentation

    Hayato Yokoi, Shunya Nakamura, Satoshi Ansai, Kiyoshi Naruse, Tohru Suzuki

    52nd Annual Meeting of the Japanese Society of Developmental Biologists (JSDB) Co-sponsored by Asia-Pacific Developmental Biology Network (APDBN)　2019/05/15

  42. Distribution of Sox10-positive chromatophore progenitors and neurons in metamorphosing flounder larvae International-presentation

    Tohru Suzuki, Minato Miyake, Kota Sato, Hayato Yokoi

    52nd Annual Meeting of the Japanese Society of Developmental Biologists (JSDB) Co-sponsored by Asia-Pacific Developmental Biology Network (APDBN)　2019/05/15

  43. 色素胞分化に関与する新規遺伝子の解析

    中村峻也, 安齋賢, 成瀬清, 鈴木徹, 横井勇人

    日本水産学会春季大会　2019/03/27

  44. エレクトロポレーションを用いた魚類への遺伝子導入効果の検討

    岩泉雅樹, 関根通陽, 横井勇人, 鈴木徹

    日本水産学会春季大会　2019/03/27

  45. トランスクリプトーム解析により明らかになったササウシノシタの変態に伴う遺伝子発現プロファイルの変化

    齋藤雄大, 横井勇人, 宇治督, 鈴木徹

    日本水産学会春季大会　2019/03/27

  46. RNA-Seq screening of up- and down-regulated genes by retinoic acid in metamorphosing flounder larvae

    陳其然, 横井勇人, 鈴木徹

    日本水産学会春季大会　2019/03/27

  47. ヒラメの着色型黒化進行に伴う脳と皮膚のtranscriptomeの変化

    齋藤雄大, 篠原怜, 松田直往, 田川正朋, 横井勇人, 鈴木徹

    日本水産学会春季大会　2019/03/27

  48. 熱ショック転写因子1の比較生物学的解析およびその局所遺伝子発現誘導方への応用

    坂本丞, 苣田英里子, Cuong Van Pham, 横井勇人, 北野健, 亀井保博

    第41回日本分子生物学会年会　2018/11/30

  49. ササウシノシタ(Heteromycteris japonica)仔魚における鰭神経回路の発 生と色素前駆細胞の局在

    菊池美希, 齋藤雄大, 遠藤優女, 陳其然, 横井勇人, 宇治督, 鈴木徹

    日本動物学会第89回札幌大会　2018/09/13

  50. HSF1の比較生物学的解析:IR-LEGOへの展開

    坂本丞, 苣田恵理子, ファム バンクオン, 横井勇人, 北野健, 亀井保博

    日本動物学会第89回札幌大会　2018/09/13

  51. Genes involved in pigment cell differentiation isolated by gene expression profiling of flounder metamorphosis International-presentation

    Hayato Yokoi, Shunya Nakamura, Laura Cal, Josep Rotllant, Tohru Suzuki

    24th Japanese Medaka and Zebrafish Meeting　2018/08/25

  52. The bamboo sole (Heteromycteris japonica)): A small-sized flatfish suitable as a laboratory model for metamorphic development in the Pleuroneciformes International-presentation

    Tohru Suzuki, Chen Qiran, Hayato Yokoi

    24th Japanese Medaka and Zebrafish Meeting　2018/08/25

  53. 変態期ヒラメの RNA-seq 解析で単離された色素胞分化に関与する遺伝子の解析

    横井勇人, Rotllant Josep, Cal Laura, 鈴木徹

    日本水産学会春季大会　2018/03/27

  54. 魚類における視交叉上核を介した体内時計の制御機構に関する研究

    茂木淳, 横井勇人, 鈴木徹

    日本水産学会春季大会　2018/03/27

  55. ヒラメ下垂体における時計遺伝子と内分泌関連遺伝子の概日リズムに関する解析

    丹羽祥太, 茂木淳, 横井勇人, 鈴木徹

    日本水産学会春季大会　2018/03/27

  56. ヒラメの着色型黒化部に現れる黒色素胞の起源について

    十川麻衣, 松田直往, 田川正朋, 横井勇人, 鈴木徹

    日本水産学会春季大会　2018/03/27

  57. Pigmentation genes isolated by transcriptome expression profiling of flounder metamorphosis

    Hayato Yokoi, Laura Cal, Josep Rotllant, Tohru Susuki

    2017年度生命科学系学会合同年次大会、第40回日本分子生物学会年会、第90回日本生化学会大会　2017/12/06

  58. トラフグの脊椎骨で椎間板様組織を構成する脊索のトランスクリプトーム解析

    鈴木徹, 明珍大志, 横井勇人

    日本動物学会 第88回大会　2017/09/21

  59. ヒラメの無眼側皮膚に異所的に出現する色素胞の由来について

    十川麻衣, 茂木淳, 横井勇人, 鈴木徹

    日本動物学会 第88回大会　2017/09/21

  60. Expression profile of RA-related genes during metamorphic development in Japanese flounder International-presentation

    Qiran Chen, Hayato Yokoi, Tohru Suzuki

    23rd Japanese Medaka and Zebrafish Meeting　2017/08/30

  61. Application of electroporation to zebrafish and bubble-eye goldfish for exogenous protein synthesis in the ovary and skeletal muscle International-presentation

    Masaki Iwaizumi, Hayato Yokoi, Tohru Suzuki

    23rd Japanese Medaka and Zebrafish Meeting　2017/08/30

  62. ヒラメ変態期の左右非対称な形態形成と細胞分化

    横井勇人, 鈴木徹

    第３回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会　2017/08/25

  63. Whole genome duplication and diversification of gene function in teleosts: lessons from Fgfr1, Sox9 and Aldh genes International-presentation

    Yokoi H, Chen Q, Cañestro C, Postlethwait JH, Suzuki T

    XI Congress of Iberian Association for Comparative Endocrinology (AIEC)　2017/07/13

  64. Transcriptome analysis of flatfish metamorphosis identified ocular side biased genes involved in pigmentation International-presentation

    Yokoi H, Kunimasa M, Sakai Y, Suzuki T

    10th European Zebrafish Meeting　2017/07/03

  65. Rhythmic per2 expression at the suprachiasmatic nucleus of the Japanese flounder, Paralichythys olivaceus, and its implications for circadian clock mechanism International-presentation

    Suzuki T, Mogi M, Yokoi H

    18th International Congress of Comparative Endocrinology (ICCE18)　2017/06/04

  66. Expression of three clock genes, per1, per2 and cry1 in the caudal fin of Japanese flounder International-presentation

    Mogi M, Yokoi H, Suzuki T

    18th International Congress of Comparative Endocrinology (ICCE18)　2017/06/04

  67. Expression profiling of Japanese flounder abnormal pigmentation caused by feeding conditions International-presentation

    Minori Kunimasa, Hayato Yokoi, Yoshifumi Sakai, Tadahisa Seikai, Tohru Suzuki

    50th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)　2017/05/10

  68. Small-scale culture system of bamboo sole (Heteromycteris japonica), a small-sized species of the Pleuroneciformes, and its metamorphic external asymmetry and pectoral fin loss International-presentation

    Tohru Suzuki, Quiran Chen, Susumu Uji, Hayato Yokoi

    50th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)　2017/05/10

  69. 給餌条件により生じるヒラメ体色異常の遺伝子発現プロファイリング解析

    國政実里, 横井勇人, 酒井義文, 青海忠久, 鈴木徹

    日本水産学会春季大会　2017/03/26

  70. ヒラメ末梢組織における時計遺伝子発現の日周リズムの観察とそれらに及ぼすコルチゾル添加の影響の解析

    茂木淳, 横井勇人, 鈴木徹

    日本水産学会春季大会　2017/03/26

  71. エレクトロポレーション法による成体へのプラスミド導入とタンパク合成誘導効果の検討

    岩泉雅樹, 横井勇人, 鈴木徹

    日本水産学会春季大会　2017/03/26

  72. ヒラメ再生皮膚に現れる色素前駆細胞と色素胞の分布

    十川麻衣, 茂木淳, 横井勇人, 鈴木徹

    日本水産学会春季大会　2017/03/26

  73. Expression of RA-receptors and RA-catabolic enzymes in Japanese flounder larvae

    陳其然, 横井勇人, 鈴木徹

    日本水産学会春季大会　2017/03/26

  74. Expression profiling of flatfish, a weird teleost fish that transforms into an asymmetric body plan International-presentation

    Hayato Yokoi, Minori Kunimasa, Youhei Washio, Xiaoming Wu, Yoshifumi Sakai, Tadahisa Seikai, Tohru Suzuki

    The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan　2016/11/14

  75. Rhythmicity of clock genes, per2, per1 and cry1, in peripheral tissue in flounder (Paralichthys olivaceus), a marine teleost fish International-presentation

    Makoto Mogi, Hayato Yokoi, Tohru Suzuki

    The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan　2016/11/14

  76. Photoentrainment of dairy rhythmicity in the SCN and peripheral tissue in teleost fish, Japanese flounder (Paralichthys olivaceus)

    茂木淳, 横井勇人, 鈴木徹

    第23回日本時間生物学会学術大会　2016/11/12

  77. Embryonic development of circadian rhythmicity in the suprachiasmatic nucleus and pineal gland in flounder (Paralichthys olivaceus), a teleost fish International-presentation

    Makoto Mogi, Hayato Yokoi, Tohru Suzuki

    International Symposium on Biological Rhythms (JSC international Symposium) “Towards understanding the molecular clockwork”　2016/11/11

  78. 次世代シーケンサーを用いた遺伝子発現プロファイリングによるヒラメの体色異常に関与する遺伝子の解析

    國政実里, 横井勇人, 青海忠久, 鈴木徹

    平成28年度日本水産学会北海道-東北合同支部大会　2016/10/22

  79. Expression of RA synthases, aldh1a1, 1a2 and 1a3, in newly hatched, metamorphic larvae and adult of Japanese founder

    陳其然, 横井勇人, 鈴木徹

    日本水産学会秋季大会　2016/09/08

  80. ヒラメの着色型黒化進行部位における色素前駆細胞の分布

    十川麻衣, 茂木淳, 横井勇人, 鈴木徹

    日本水産学会秋季大会　2016/09/08

  81. ブラジル産とユタ産アルテミアを与えたヒラメ変態期仔魚で発現量の異なる遺伝子のRNA-Seqによるスクリーニング

    國政実里, 青海忠久, 鈴木徹, 横井勇人

    日本水産学会秋季大会　2016/09/08

  82. DENによるメダカ再生鰭でのvasa, nanosの発現誘導

    Jialing Shen, 横井勇人, 鈴木徹

    日本水産学会秋季大会　2016/09/08

  83. TALEN法を用いて作製したr-spondin2変異体ゼブラフィッシュの表現型

    岩泉雅樹, 辰見吉昭, 武田萌, 鈴木徹, 横井勇人

    日本ゲノム編集学会第1回大会　2016/09/06

  84. ヒラメ：左右非対称な眼の移動と色素胞分化

    横井勇人

    第２回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会　2016/08/21

  85. Transcriptome analysis of flounder metamorphosis International-presentation

    Hayato Yokoi, Minori Kunimasa, Yoshifumi Sakai, Tohru Suzuki

    22nd Japanese Medaka and Zebrafish Meeting　2016/08/20

  86. 変態期ヒラメの眼球移動に関与する遺伝子の探索

    横井勇人, 酒井義文, 鈴木徹

    日本水産学会春季大会　2016/03/26

  87. Induction of germline-specic and pluripotency factor mRNAs, including vasa and oct4, in medaka somatic cells by diethylnitrosamine

    Shen Jialing, Hayato Yokoi, Tohru Suzuki

    日本水産学会春季大会　2016/03/26

  88. Vitamine A metabolism, revealed by RNA-seq, in the notochord constituting intervertebral tissue

    Khalid Freeha, Hayato Yokoi, Tohru Suzuki

    日本水産学会春季大会　2016/03/26

  89. ヒラメにおける時計遺伝子の日周リズムおよび光同調性の解析

    茂木淳, 横井勇人, 鈴木徹

    日本水産学会春季大会　2016/03/26

  90. エレクトロポレーションによる成体器官への遺伝子導入効果の検討

    岩泉雅樹, 横井勇人, 鈴木徹

    日本水産学会春季大会　2016/03/26

  91. ヒラメにおける鱗の発生と色素胞、色素前駆細胞の挙動

    十川麻衣, 茂木淳, 横井勇人, 鈴木徹

    日本水産学会春季大会　2016/03/26

  92. ヒラメを用いた魚類概日リズムの光同調機構の解析

    茂木淳, 宇治督, 横井勇人, 鈴木徹

    BMB2015（第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会）　2015/12/01

  93. ヒラメにおける視交叉上核、松果体および体細胞の概日リズムの発生

    茂木淳, 宇治督, 横井勇人, 鈴木徹

    日本水産学会秋季大会　2015/09/22

  94. 変態期ヒラメ頭部で左右非対称に発現する遺伝子の探索

    横井勇人, 久保田直喜, 烏暁明, 酒井義文, 鈴木徹

    日本水産学会秋季大会　2015/09/22

  95. Generation of tumor protein p53, tp53, Knockout Medaka Using an oct4-gfp/β-actin-dsred Double Transgenic Line International-presentation

    Jialing Shen, Shinpei Yokota, Hiroyuki Kato, Masato Kinoshita, Hayato Yokoi, Tohru Suzuki

    21st Japanese Medaka and Zebrafish Meeting　2015/09/19

  96. ヒラメ・カレイ類の左右非対称な体色形成と着色異常の発生機構

    鈴木徹, 鷲尾洋平, 烏暁明, 國政実里, 十川麻衣, 横井勇人

    日本動物学会 第86回大会　2015/09/17

  97. 変態期ヒラメの左右非対称化の分子基盤を解明したい：RNA seqによるアプローチ

    横井勇人

    ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会　2015/08/18

  98. 左ヒラメと右カレイを分ける発生システム

    鷲尾洋平, 横井勇人, 鈴木徹

    日本動物学会 平成27年度東北支部会　2015/08/08

  99. Effects of light conditions on the development of circadian rhythm in the suprachiasmatic nuclei and pineal in embryos of a marine fish, flounder (Paralichthys olivaceus). International-presentation

    Makoto Mogi, Susumu Uji, Hayato Yokoi, Tohru Suzuki

    48th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)　2015/06/02

  100. Post-metamorphic ectopic pigmentation at the ocular side skin in flatfish International-presentation

    Tohru Suzuki, Minori Kunimasa, Xiaoming Wu, Mai Togawa, Susumu Uji, Hayato Yokoi

    48th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)　2015/06/02

  101. Transcriptome analysis of asymmetric pigmentation in the flounder metamorphosis International-presentation

    Hayato Yokoi, Minori Kunimasa, Xiaoming Wu, Yoshifumi Sakai, Tohru Suzuki

    48th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)　2015/06/02

  102. TALEN 法による2種類の r-spondin 2 変異体ゼブラフィッシュの作製と表現型解析

    武田萌, 辰見吉昭, 松田勝, 鈴木徹, 横井勇人

    日本水産学会春季大会　2015/03/27

  103. ヒラメ仔魚の有眼側/無眼側の遺伝子発現プロファイリングで単離された色素胞関連遺伝子

    横井勇人, 國政実里, 烏暁明, 酒井義文, 鈴木徹

    日本水産学会春季大会　2015/03/27

  104. ヒラメ体色形成における dhfr遺伝子の色素前駆細胞マーカーとしての有用性の検討

    國政実里, 烏暁明, 横井勇人, 鈴木徹

    日本水産学会春季大会　2015/03/27

  105. ヒラメ・カレイ類の変態後に起こる無眼側着色型黒化の発生過程と色素胞前駆細胞の分布

    鈴木徹, 烏暁明, 國政実里, 横井勇人, 宇治督, 礒島俊実, 田川正朋

    日本水産学会春季大会　2015/03/27

  106. レチノイン酸によりヒラメ仔魚に発現誘導される遺伝子の次世代シーケンサーを使ったスクリーニング

    烏暁明, 横井勇人, 鈴木徹

    日本水産学会春季大会　2015/03/27

  107. 魚類におけるゲノム倍加と種特異的多様化

    横井勇人

    第23回 関西おさかな勉強会　2014/12/05

  108. ヒラメ視交叉上核での概日リズムの発生

    茂木淳, 宇治督, 横井勇人, 鈴木徹

    第37回 日本分子生物学会年会　2014/11/25

  109. Expression profiling of flounder metamorphosis identifies genes involved in pigment cell differentiation. International-presentation

    Hayato Yokoi, Minori Kunimasa, Xiaoming Wu, Youhei Washio, Yoshifumi Sakai, Tohru Suzuki

    20th Japanese Medaka and Zebrafish Meeting　2014/09/20

  110. oct4-gfp Tgメダカ系統の作製と胚発生、鰭再生過程におけるGFP発現の特性解析

    松野林太, 横田晋平, 加藤寛之, 横井勇人, 鈴木徹

    日本水産学会秋季大会　2014/09/19

  111. ヒラメ視交叉上核及び松果体での概日リズムの発生

    茂木淳, 宇治督, 横井勇人, 鈴木徹

    日本水産学会秋季大会　2014/09/19

  112. AIDとMBD4による脱メチル化はゼブラフィッシュ胚で起こるのか?

    下田修義, 廣瀬健太朗, 井澤俊明, 横井勇人, 橋本有弘, 菊池裕

    日本遺伝学会第86回大会　2014/09/17

  113. ヒラメ視交叉上核での分子時計の初期発生

    茂木淳, 宇治督, 横井 勇人, 鈴木徹

    日本動物学会 第85回大会　2014/09/11

  114. Transcriptome analysis identified genes enriched in ocular- or blind-side of flounder International-presentation

    H. Yokoi, X. Wu, M. Kunimasa, Y. Sakai, T. Suzuki

    11th International Conference on Zebrafish Development and Genetics　2014/06/24

  115. Identification of ocular- or blind-side enriched genes in flounder metamorphosis International-presentation

    Hayato Yokoi, Xiaoming Wu, Yoshifumi Sakai, Tohru Suzuki

    47th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)　2014/05/27

  116. TALEN mediated knockout zebrafish reveals the critical function of r-spondin2 in the fin and vertebral skeletogenesis International-presentation

    Tatsumi Yoshinari, Hayato Yokoi, Masaru Matsuda, Tohru Suzuki

    47th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)　2014/05/27

  117. Development of circadian rhythm in the suprachiasmatic nuclei in a marine fish, flounder (Paralichthys olivaceus) embryos International-presentation

    Makoto Mogi, Susumu Uji, Hayato Yokoi, Tohru Suzuki

    47th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)　2014/05/27

  118. ゼブラフィッシュ胚でAIDとMBD4がカップルした脱メチル化は起こるのか?

    下田修義, 廣瀬健太朗, 井澤俊明, 横井勇人, 橋本有弘, 菊池裕

    日本エピジェネティクス研究会　2014/05/25

  119. メダカ系統を使った臆病な性質の定量化の試み

    明珍大志, 橋本寿史, 横井勇人, 鈴木徹

    日本水産学会春季大会　2014/03/27

  120. TALEN 法による甲状腺ホルモン欠損メダカの作出と表現型解析

    阿部雄二, 松田勝, 鈴木徹, 横井勇人

    日本水産学会春季大会　2014/03/27

  121. TALEN 法による r-spondin 2ノックアウトゼブラフィッシュの作製と表現型解析

    辰見吉昭, 武田萌, 松田勝, 横井勇人, 鈴木徹

    日本水産学会春季大会　2014/03/27

  122. ヒラメにおける概日リズムの発生と照明の影響

    茂木淳, 宇治督, 横井勇人, 鈴木徹

    日本水産学会春季大会　2014/03/27

  123. レチノイン酸により誘導される無眼側黒化現象の分子マーカーを用いた解析

    烏暁明, 鷲尾洋平, 宇治督, 横井勇人, 鈴木徹

    日本水産学会春季大会　2014/03/27

  124. ヒラメ仔魚の有眼側と無眼側で非対称に発現する遺伝子の次世代シーケンサーによる解析

    横井勇人, 烏暁明, 酒井義文, 鈴木徹

    日本水産学会春季大会　2014/03/27

  125. 薬物代謝遺伝子の発現を指標にした汚染状況の生物モニタリング法の開発と津波後干潟域の汚染調査

    鷲尾洋平, 宗像拓也, 横井勇人, 鈴木徹

    日本水産学会春季大会　2014/03/27

  126. Flounder eye-sidedness can be experimentally randomized by transient treatment with a Nodal antagonist SB-431542 at somite stage

    Youhei Washio, Hayato Yokoi, Tohru Suzuki

    第36回 日本分子生物学会年会　2013/12/03

  127. 脊椎動物モデルとしての小型魚類と TALEN 革命

    横井勇人, 辰見吉昭, 阿部雄二, 武田萌, 松田勝, 鈴木徹

    日本動物学会 第84回大会　2013/09/26

  128. ヒラメを対象にした次世代シークエンサーを使った de novo トランスクリプトーム解析

    鈴木徹, 茨木春美, 烏暁明, 酒井義文, 横井勇人

    日本動物学会 第84回大会　2013/09/26

  129. メダカとゼブラフィッシュで作製した Takifugu-oct4/egfp トランスジェニック

    横田真平, 加藤寛之, 橋本寿史, 横井勇人, 鈴木徹

    日本動物学会 第84回大会　2013/09/26

  130. ササウシノシタ(真骨魚類、異体類)の実験室内飼育と地域集団間での遺伝的多型解析

    高木雅子, 宇治督, 横井勇人, 鈴木徹

    日本動物学会 第84回大会　2013/09/26

  131. ヒラメの初期仔魚を使った慨日リズムの発生に関する解析

    茂木淳, 宇治督, 横井勇人, 鈴木徹

    日本動物学会 第84回大会　2013/09/26

  132. 次世代シーケンサーを用いたヒラメ脊椎骨の de novo トランスクリプトーム解析

    茨木春美, 烏暁明, 横井勇人, 酒井義文, 鈴木徹

    日本動物学会 第84回大会　2013/09/26

  133. Analysis of developmental process of molecular clocks at the suprachiasmatic nuclei and pineal gland using flounder Paralichthys olivaceus as a model organism International-presentation

    Makoto Mogi, Susumu Uji, Hayato Yokoi, Tohru Suzuki

    19th Japanese Medaka and Zebrafish Meeting　2013/09/20

  134. lounder eye-sidedness can be experimentally randomized by transient treatment with a Nodal antagonist SB-431542 at somite stage International-presentation

    Youhei Washio, Hayato Yokoi, Tohru Suzuki

    19th Japanese Medaka and Zebrafish Meeting　2013/09/20

  135. Transcriptome analyses of flounder metamorphosis left-right asymmetric differentiation International-presentation

    Hayato Yokoi, Xiaoming Wu, Kei Sawada, Yoshifumi Sakai, Tohru Suzuki

    46th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)　2013/05/29

  136. ヒラメでは変態期に一過的にzic1（double anal fin）の発現が有眼側皮膚で、チューブリンのアセチル化が無眼側皮膚で起こる

    鈴木徹, 鷲尾洋平, 烏暁明, 宇治督, 横井勇人

    日本水産学会春季大会　2013/03/27

  137. 胚期に行ったノダル抑制剤処理によって発生するヒラメの眼位逆位

    鷲尾洋平, 福田慎作, 藤浪祐一郎, 横井勇人, 鈴木徹

    日本水産学会春季大会　2013/03/27

  138. ヒラメ仔魚における色素胞前駆細胞の体幹正中部から体表への移動経路と左右差形成

    鷲尾洋平, 藤浪祐一郎, 清水大輔, 横井勇人, 鈴木徹

    日本水産学会春季大会　2013/03/27

  139. レチノイン酸により誘起される脊椎骨異常の進行過程の解析

    烏暁明, 鷲尾洋平, 宇治督, 横井勇人, 鈴木徹

    日本水産学会春季大会　2013/03/27

  140. メダカを使ったチログロブリン-ノックアウトフィッシュ作出の試み

    阿部雄二, 亀井保博, 谷口善仁, 松田勝, 鈴木徹, 横井勇人

    日本水産学会春季大会　2013/03/27

  141. R-spondin の発現解析と TALEN 法によるノックアウトフィッシュの作成

    辰見吉昭, 松田勝, 鈴木徹, 横井勇人

    日本水産学会春季大会　2013/03/27

  142. 変態期ヒラメ仔魚のトランスクリプトーム解析

    横井勇人, 烏暁明, 酒井義文, 鈴木徹

    日本水産学会春季大会　2013/03/27

  143. ヒラメにおける中枢時計と生体リズムの初期発生

    茂木淳, 渡辺奈々子, 宇治督, 横井勇人, 鈴木徹

    日本水産学会春季大会　2013/03/27

  144. ササウシノシタの受精卵から成魚までの実験室閉鎖系システムでの飼育

    高木雅子, 宇治督, 青海忠久, 横井勇人, 鈴木徹

    日本水産学会春季大会　2013/03/27

  145. oct4-gfp Tg ゼブラフィッシュに由来する胚細胞の培養

    加藤寛之, 横田晋平, 宇治督, 横井勇人, 鈴木徹

    日本水産学会春季大会　2013/03/27

  146. ヒラメ脊椎骨で発現する遺伝子の次世代シークエンス解析によるトランスクリプトーム

    茨木春美, 烏暁明, 鷲尾洋平, 宇治督, 横井勇人, 酒井義文, 鈴木徹

    日本水産学会春季大会　2013/03/27

  147. TALEN によるゲノムへの変異の導入

    鈴木徹, 辰見吉昭, 阿部雄二, 松田勝, 横井勇人

    アクアゲノム研究会　2013/03/26

  148. 次世代シーケンサーを用いたヒラメのトランスクリプトーム解析の試み

    横井勇人, 茨木春美, 烏暁明, 鷲尾洋平, 宇治督, 酒井義文, 鈴木徹

    アクアゲノム研究会　2013/03/26

  149. 小型魚類の発生遺伝学と脊椎動物の進化

    横井勇人

    先端農学セミナー　2013/02/15

  150. Evolutionary Diversification of Duplicated Copies of Pomc in Teleost Fish

    Hayato Yokoi, Mika Bannai, Tohru Suzuki

    第３５回日本分子生物学会年会　2012/12/11

  151. Expression of Molecular Clock Genes, per2 and bmal1, in the Suprachiasmatic Nuclei and Habenulae of Flounder, Paralichthys Olivaceus, Larvae

    Tohru Suzuki, Makoto Mogi, Nanako Watanabe, Hayato Yokoi

    第３５回日本分子生物学会年会　2012/12/11

  152. The analyses of gene expression and function of G-protein coupled receptor, lgr4, and its ligand, r-spondin2, using zebrafish embryos International-presentation

    Yoshiaki Tatsumi, Hayato Yokoi, Tohru Suzuki

    18th Japanese Medaka and Zebrafish Meeting　2012/09/22

  153. Expression of molecular clock genes, per2 and bmal1, in the suprachiasmatic nuclei and habenulae of flounder, Paralichthys olivaceus, larvae International-presentation

    Tohru Suzuki, Nanako Watanabe, Makoto Mogi, Susumu Uji, Hayato Yokoi

    18th Japanese Medaka and Zebrafish Meeting　2012/09/22

  154. Characterization of thyroid grand development in medaka, and isolation of thyroglobulin mutant using TILLING International-presentation

    Yuji Abe, Yoshihito Taniguchi, Yasuhiro Kamei, Kiyoshi Naruse, Tohru Suzuki, Hayato Yokoi

    18th Japanese Medaka and Zebrafish Meeting　2012/09/22

  155. 遺伝子マーカーを併用したヒラメ稚魚の発生ステージングの検討

    烏暁明, 鷲尾洋平, 宇治督, 横井勇人, 鈴木徹

    日本水産学会秋季大会　2012/09/14

  156. ゲノム倍加によって生じた２つの魚類pomc遺伝子の多様性

    横井勇人, 坂内美香, 鈴木徹

    日本進化学会 第14回東京大会　2012/08/21

  157. ゼブラフィッシュの加齢はゲノミックとエピゲノミックな変化を伴う

    下田修義, 井澤俊明, 吉澤明生, 横井勇人, 菊池裕, 橋本有弘

    日本基礎老化学会　2012/07/26

  158. Thyroid grand development in medaka; toward a model to study metamorphosis

    Hayato Yokoi, Yuji Abe, Tohru Suzuki

    Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology　2012/05/28

  159. Development of circadian clock in the suprachiasmatic nuclei of teleost fish

    Nanako Watanabe, Yuichiro Fujinami, Daisuke Shimizu, Susumu Uji, Hayato Yokoi, Tohru Suzuki

    Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology　2012/05/28

  160. Localization of the precursor cells of adult-type chromatophores in the ocular side precedes asymmetric body coloration in metamorphosing flounder larvae

    Youhei Washio, Wu Xiaoming, Hayato Yokoi, Yuichiro Fujinami, Daisuke Shimizu, Susumu Uji, Tohru Suzuki

    Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology　2012/05/28

  161. ゼブラフィッシュの加齢はゲノミックとエピゲノミックな変化を伴う

    下田修義, 井澤俊明, 吉澤明生, 横井勇人, 菊池裕, 橋本有弘

    日本エピジェネティクス研究会　2012/05/14

  162. 時計遺伝子 period2 の魚類視交叉上核における発現

    渡辺奈々子, 伊藤香絵, 宇治督, 横井勇人, 鈴木徹

    日本水産学会春季大会　2012/03/26

  163. 仔魚期の 24 時間照明飼育で起こる視床下部・下垂体経路の異常

    伊藤香絵, 鷲尾洋平, 清水大輔, 藤浪祐一郎, 宇治督, 横井勇人, 鈴木徹

    日本水産学会春季大会　2012/03/26

  164. メダカにおける甲状腺関連マーカー遺伝子の発現による甲状腺正常発生の観察

    阿部雄二, 鈴木徹, 横井勇人

    日本水産学会春季大会　2012/03/26

  165. oct4 promoter/gfp-Tg ゼブラフィッシュとメダカを使った細胞の多能性モニター

    加藤寛之, 阿部光太, 宇治督, 橋本寿史, 横井勇人, 鈴木徹

    日本水産学会春季大会　2012/03/26

  166. 異体類の成体型色素胞前駆細胞の左右非対称な出現パターン

    鷲尾洋平, 近藤大地, 藤浪祐一郎, 清水大輔, 宇治督, 横井勇人, 鈴木徹

    日本水産学会春季大会　2012/03/26

  167. ヒラメ仔魚で左右非対称に発現する遺伝子の単離の試み

    横井勇人, 近藤大地, 藤浪祐一朗, 宇治督, 鈴木徹

    日本水産学会春季大会　2012/03/26

  168. Circadian pacemaker in the suprachiasmatic nuclei of teleost fish revealed by rhythmic per2 expression International-presentation

    Tohru Suzuki, Nanako Watanabe, Hayato Yokoi

    International Symposium: Frontiers in Behavioral Brain Science ~Solving the mystery of sleep~　2012/03/19

  169. トラフグ脊椎骨形成過程の観察と脊索形成関連および椎骨形成関連遺伝子の発現解析

    鈴木徹, 金子隆政, 宇治督, 横井勇人

    日本分子生物学会年会　2011/12/13

  170. 魚類におけるゲノム倍加と機能分配

    横井勇人

    アクアゲノム研究会　2011/10/01

  171. Molecular cloning of period2 from flounder, and analysis of its expression sites in the larval brain and dairy rhythm International-presentation

    Nanako Watanabe, Hayato Yokoi, Tohru Suzuki

    17th Japanese Medaka and Zebrafish Meeting　2011/09/08

  172. A trial to dissect the flounder “hentai”: isolation of genes expressed asymmetrically in left or right side in flounder International-presentation

    Hayato Yokoi, Daichi Kondo, Yuichiro Fujinami, Tohru Suzuki

    17th Japanese Medaka and Zebrafish Meeting　2011/09/08

  173. Isolation of genes enriched asymmetrically in left or right side in flounder

    Hayato Yokoi, Daichi Kondo, Xiaoming Wu, Yuichiro Fujinami, Tohru Suzuki

    44th Annual Meeting for the Japanese Society of Developmental Biologists　2011/05/18

  174. Analysis on the relationship between asymmetric expression of pax6 in the habenulae and the Nodal pathway in flounder

    Yohei Washio, Daichi Kondo, Masato Aritaki, Yuichiro Fujinami, Daisuke Shimizu, Hayato Yokoi, Tohru Suzuki

    44th Annual Meeting for the Japanese Society of Developmental Biologists　2011/05/18

  175. Establishment of a transgenic zebrafish line, carrying fugu oct3/4 promoter-GFP, for monitoring pluripotency of the cells

    Kota Abe, Hayato Yokoi, Tohru Suzuki

    44th Annual Meeting for the Japanese Society of Developmental Biologists　2011/05/18

  176. Left-sided expression of pitx2 at dorsal diencephalon and gut is maintained throughout larval development in flounders for metamorphic morphogenesis

    Daichi Kondo, Yuichiro Fujinami, Susumu Uji, Hisashi Hashimoto, Hayato Yokoi, Tohru Suzuki

    44th Annual Meeting for the Japanese Society of Developmental Biologists　2011/05/18

  177. トラフグの背腹色素パターンの形成過程と背腹で発現強度の異なる色素形成関連遺伝子のスクリーニング

    Wu Xiaoming, 宇治督, 横山芳博, 横井勇人, 鈴木徹

    日本水産学会春季大会　2011/03/27

  178. 24時間照明飼育で起こる異体類の体色異常に関する研究

    伊藤香絵, 渡辺奈々子, 鷲尾洋平, 清水大輔, 藤浪祐一郎, 宇治督, 横井勇人, 鈴木徹

    日本水産学会春季大会　2011/03/27

  179. ヒラメ・カレイ類の左右非対称性を制御するpitx2の変態期再発現と、再発現に関連する左特異的エンハンサー内の核受容体応答配列

    鈴木徹, 近藤大地, ウショーミン, 横井勇人, 宇治督, 橋本寿史

    日本分子生物学会年会 日本生化学会大会 合同大会　2010/12/07

  180. oct4-GFPトランスジェニックゼブラフィッシュによる細胞の多能性の可視化

    阿部光太, 横井勇人, 橋本寿史, 鈴木徹

    日本水産学会 東北支部大会　2010/11/05

  181. 左ヒラメと右カレイを振り分ける発生システムについての解析

    鈴木徹, 横井勇人, 宇治督, 橋本寿史, 田中克

    第16回 小型魚類研究集会　2010/09/18

  182. Evolution of soxE genes in teleost fish revealed by comparative expression analysis and genomics

    Hayato Yokoi, Yi-Lin Yan, Ayano Miyagi, Mark C. Currey, Julian M. Catchen, William A. Cresko, Tohru Suzuki, John H. Postlethwait

    43rd Annual Meeting for the Japanese Society of Developmental Biologists　2010/06/20

  183. ゲノム倍加とゼブラフィッシュsox8, sox9の進化

    横井勇人, Yi-Lin Yan, 宮城絢乃, Julian M. Catchen, Mark C. Currey, William A. Cresko, 鈴木徹, John H. Postlethwait

    日本水産学会春季大会　2010/03/26

  184. トラフグをモデルにした脊椎骨形成過程と脊椎骨発生関連遺伝子の発現解析

    金子隆政, 横井勇人, 鈴木徹, 宇治督

    日本水産学会春季大会　2010/03/26

  185. 真骨魚類におけるゲノム倍加とSox9の機能分配

    横井勇人

    シンポジウム「仙台・動物発生コロキウム2010」　2010/01/22

  186. 魚類におけるゲノム倍加と種特異的多様化

    横井勇人

    海洋生物科学系セミナー　2009/10/30

  187. Sox9の下流遺伝子の探索とゼブラフィッシュsox9a, sox9bへの機能の分配

    横井勇人, Yi-Lin Yan, Michael R. Miller, Julian M. Catchen, Eric A. Johnson、John, H. Postlethwait

    日本水産学会秋季大会　2009/09/30

  188. Subfunction partitioning of Sox9 in zebrafish revealed by analysis of down-stream target genes.

    Hayato Yokoi, Yi-Lin Yan, Michel Miller, Julian Catchen, Eric Johnson, John Postlethwait

    42nd Annual Meeting for the Japanese Society of Developmental Biologists　2009/05/28

  189. Subfunction partitioning of Sox9 into sox9a and sox9b revealed by analysis of down-stream targets. International-presentation

    Hayato Yokoi, YiLin Yan, Michael R. Miller, Eric A. Johnson, John H. Postlethwait

    8th International Conference on Zebrafish Development and Genetics　2008/06/25

  190. Expression profiling of mutant embryos shows that Sox9 is essential for retinal differentiation. International-presentation

    Hayato Yokoi, YiLin Yan, Michael R. Miller, Eric A. Johnson, John H. Postlethwait

    Northwest Regional Developmental Biology Conference　2008/03/12

  191. Site-specific integration of large DNA fragments into the zebrafish genome using phiC31-integrase. International-presentation

    Michael R. Miller, Hayato Yokoi, Chris Q. Doe, John H. Postlethwait

    2nd GSA Genetic Analysis: Model Organisms to Human Biology Meeting　2008/01/05

  192. Identifying downstream targets of Sox9 by expression profiling of single and double mutant zebrafish embryos. International-presentation

    Hayato Yokoi, Michael R. Miller, YiLin Yan, Eric A. Johnson, John H. Postlethwait

    7th International Conference on Zebrafish Development and Genetics　2006/06/14

  193. How did the duplication of zebrafish sox9 coorthologs alter regulatory interactions with downstream targets? International-presentation

    YiLin Yan, XinJun He, Hayato Yokoi, Amy Singer, John H. Postlethwait

    7th International Conference on Zebrafish Development and Genetics　2006/06/14

  194. Diversified function of the conserved ligand-receptor pair FGF8-FGFR1 is revealed by the medaka fgfr1 mutant headfish. International-presentation

    H. Yokoi, D. Kobayashi, S. Takashima, T. Narita, T. Jindo, T. Kimura, T. Kitagawa, T. Kage, A. Sawada, K. Naruse, S. Asakawa, N. Shimizu, H. Mitani, A. Shima, M. Tsutsumi, H. Hori, Y. Ishikawa, Y. Saga, H. Takeda, K. Araki

    West Coast Zebrafish Meeting　2005/09/09

  195. Analysis of a medaka mutant headfish, defective in trunk and tail development.

    H. Yokoi, D. Kobayashi, S. Takashima, T. Narita, T. Jindo, T. Kimura, T. Kitagawa, T. Kage, A. Sawada, K. Naruse, S. Asakawa, N. Shimizu, H. Mitani, A. Shima, M. Tsutsumi, H. Hori, Y. Ishikawa, Y. Saga, H. Takeda, K. Araki

    第27回日本分子生物学会年会　2004/12/08

  196. Analysis of a medaka mutant headfish, defective in trunk and tail development. International-presentation

    H. Yokoi, D. Kobayashi, S. Takashima, T. Narita, T. Jindo, T. Kimura, T. Kitagawa, T. Kage, A. Sawada, K. Naruse, S. Asakawa, N. Shimizu, H. Mitani, A. Shima, M. Tsutsumi, H. Hori, Y. Ishikawa, Y. Saga, H. Takeda, K. Araki

    6th International Conference on Zebrafish Development & Genetics　2004/07/29

  197. A transposon mediated gene trap approach in medaka. International-presentation

    Clemens Grabher, Alexander Arenz, Hayato Yokoi, Shigeo Takashima, Hiroyuki Takeda, Zoltan Ivics, Joachim Wittbrodt

    6th International Conference on Zebrafish Development & Genetics　2004/07/29

  198. Medaka temperature-sensitive chordin mutant reveals a novel role in left-right axis determination. International-presentation

    S. Takashima, D. Kobayashi, H. Yokoi, T. Narita, T. Jindo, T. Kage, T. Kitagawa, T. Kimura, K. Sekimizu, R. Murakami, D. Satiamarga, K. Miyake, S. Ooki, K. Kakihara, S. Tsuda, K. Naruse, H. Mitani, A. Shima, Y. Ishikawa, K. Araki, Y. Saga, H. Takeda

    6th International Conference on Zebrafish Development & Genetics　2004/07/29

  199. メダカ胴尾部欠損変異体headfishの解析

    横井勇人, 小林大介, 高島茂雄, 成田貴則, 神藤智子, 木村哲晃, 北川忠生, 景崇洋, 澤田篤志, 成瀬清, 浅川修一, 清水信義, 三谷啓志, 嶋昭紘, 堤真紀子, 堀寛, 石川裕二, 相賀裕美子, 武田洋幸, 荒木和男

    日本発生生物学会第37回大会　2004/06/04

  200. Analysis of a medaka mutant, tail-less, defective in trunk and tail development. International-presentation

    H. Yokoi, T. Kage, T. Kitagawa, T. Kimura, D. Kobayashi, A. Sawada, T. Jindo, S. Takashima, T. Narita, K. Naruse, M. Tanaka, H. Mitani, A. Shima, Y. Ishikawa, Y. Saga, H. Takeda, K. Araki

    3rd European Conference on Zebrafish and Medaka Genetics and Development　2003/06/11

  201. Positional cloning of the alad gene from a hypochromic mutant, who. International-presentation

    D. Sakamoto, K. Inohaya, H. Yokoi, T. Narita, K. Naruse, H. Mitani, A. Shima, Y. Ishikawa, Y. Imai, A. Kudo

    3rd European Conference on Zebrafish and Medaka Genetics and Development　2003/06/11

  202. メダカ胴尾部欠損変異体tail-lessの解析

    横井勇人, 景崇洋, 北川忠生, 木村哲晃, 小林大介, 澤田篤志, 神藤智子, 高島茂雄, 成田貴則, 成瀬清, 田中実, 三谷啓志, 嶋昭紘, 石川裕二, 相賀裕美子, 武田洋幸, 荒木和男

    第25回日本分子生物学会年会　2002/12/11

  203. メダカにおけるＸ線及びＥＮＵによる誘発突然変異

    保田隆子, 横井勇人, 猪早敬二, 坂本大悟, 市川久美, 太田宗宏, 成田貴則, 青木一子, 笹沼基恵, 松本厚子, 石川裕二

    日本放射線影響学会第44回大会　2001/10/29

  204. Conservation and diversification of sox9 expression in medaka.

    H. Yokoi, K. Morohashi, T. Kobayashi, M. Tanaka, Y. Nagahama, Y. Wakamatsu, H. Takeda, K. Araki, K. Ozato

    第7回小型魚類研究集会　2001/08/04

  205. Evolutional significance of medaka sox9 in gonad and cartilage development. International-presentation

    H. Yokoi, K. Morohashi, T. Kobayashi, M. Tanaka, Y. Nagahama, Y. Wakamatsu, H. Takeda, K. Araki, K. Ozato

    14th International Congress of Developmental Biology　2001/07/08

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Research Projects 20

  1. 異体類変態期の形態形成とホルモンによる制御機構 Competitive

    2009/09 - Present

  2. 小型魚類を用いた魚類変態機構の研究モデル系の構築 Competitive

    2009/09 - Present

  3. 魚類変態に関与する遺伝子の進化ゲノム学的研究 Competitive

    2009/09 - Present

  4. Functional analysis of Sox3 and Cyp26 during blind side differentiation in Japanese flounder

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2024/04/01 - 2027/03/31

  5. 新規強制発現系による二枚貝の性分化制御因子の探索と表現型解析

    長澤 一衛, 横井 勇人

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2023/04/01 - 2027/03/31

  6. 新規強制発現系による二枚貝の性分化制御因子の探索と表現型解析

    長澤 一衛, 横井 勇人

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2023/04/01 - 2027/03/31

  7. Asymmetric differentiation of pigment cells and its regulation by retinoic acid signaling in Japanese flounder

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2021/04/01 - 2024/03/31

  8. Analyses of shellfish hematopoiesis using single-cell RNA-Seq and genetic engineering technology

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2020/04/01 - 2023/03/31

  9. Molecular mechanisms of flounder metamorphosis regulating the pigment cell differentiation in ocular side and inhibiting it in blind side

    Yokoi Hayato

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2018/04/01 - 2022/03/31

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    Japanese flounder is one of the important fish species because of their commercial value, however, abnormal pigmentation is problematic in the aquaculture production. Aim of this study is to investigate the molecular mechanisms underlying the left/right asymmetric pigment cell differentiation. Genes involved in pigmentation were isolated by comparing gene expression profile of ocular side and blind side of flounder. Gene expression analysis using abnormal pigment fish with ectopic pigmentation revealed that these genes were expressed in such ectopic pigment sites and peripheries, which suggested that these genes also involved in ectopic pigmentation. In order to investigate the function of these genes, GFP-knock in strains were generated by using CRISPR/Cas9 genome editing system: gene expression can be monitored in live embryos with the GFP fluorescence.

  10. 変態発生制御のシグナル回路と形態異常感受性に関する研究

    鈴木 徹, 酒井 義文, 田川 正朋, 横井 勇人

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2017/04/01 - 2021/03/31

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    私達は，これまで異体類の発生とその内分泌調節について検討し，変態に伴う眼球移動や左右非対称な体色形成、骨形成はレチノイン酸(RA)，甲状腺ホルモン，コルチゾル等の内分泌シグナルにより制御され，種苗の形態異常(眼位や体色異常，骨異常)は，これらシグナル回路の攪乱によって発生することを示唆している。本研究では，飼育技術と育種の両面から健苗育成に貢献することを目的として、これまでの研究を発展し，変態を調節するシグナル回路を解明した上で，種苗で頻発する無眼側黒化の発生原因となるシグナル回路の乱れを究明し、さらに無眼側黒化のリスク遺伝子を特定することをめざす。本年度は、ヒラメ・カレイ類の変態期に起こる有眼側限定的な色素胞の分化の発生制御機構を解明することに成功した。すなわち色素胞の前駆細胞は、変態期初期に幹細胞に近い状態で脊髄周辺に発生する。この時、幹細胞は、神経堤細胞のマーカー遺伝子であるSox10を発現し、このSox10の発現誘導にWntシグナルが働くことが明らかになった。脊髄周辺で分化したSox10陽性幹細胞は、脊髄から伸びる神経軸索に沿って背鰭基部に到達し、さらにそこから伸びる神経に沿って左右の皮膚に移動する。幹細胞から色素胞への分化はRA依存的であり、無眼側ではRAの分解酵素であるcyp26bが強発現して色素胞の分化が抑制され、有眼側のみ着色することを明らかにした。RA合成酵素は変態期に腸管上皮で強発現することから、腸管で合成されたRAは血流を介して体全体に拡散して色素胞の分化を誘導するが、無眼側ではcyp26bにより分解されることで色素分化に左右差が発生することが示唆された。 無眼側黒化のリスク遺伝子を特定するための基盤整備として、ヒラメのほぼ全遺伝子について、cDNAの塩基配列を解読し、塩基配列の一塩基多型の情報を得るために複数個体のcDNA塩基配列を取得した。

  11. 魚類の小型・早期成熟化技術の創出－海産魚の発生工学と育種の時短に向けて－

    鈴木 徹, 宇治 督, 横井 勇人

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2019/06/28 - 2020/03/31

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    私達は、ヒラメ・カレイ類の一種であるササウシノシタが海産魚の中では小型の部類であり、かつ産卵期間が長いことに着目し、海産魚のモデル生物として利用するための研究を進めている．これまでに周年産卵システムや研究用小型飼育システムを立ち上げるなど準備し、現在、養殖魚の種苗生産で問題となっている体色や骨格の形態異常の発生機序について研究を行っている。より分子レベルで踏み込んだ研究に着手するために、発生工学や遺伝学の技術を使いたいと望んでいるが、受精から産卵まで1年と世代サイクルが長いことが障壁となっている。似たように大型の養殖対象魚種はいずれも産卵までの期間が3年と長く、育種が難しいという問題がある。 私達は、上記の研究と並行して、ゼブラフィッシュを使って、成熟雌への腹腔内注射によるゲノム編集技術の開発を行っており、実用化に近づいている。このような背景から、「開発中のゲノム編集技術を使って、ササウシノシタを小型・早期成熟化し」、ゼブラフィッシュ並に便利なモデル生物に改変することを着想し、そのための計画を立案した。本研究は、養殖対象魚の育種を省スペース・時短化するための試験研究としての意義も大きい． 本年度は、3つの課題を検討した。ゲノム編集を進めるためには、ゲノムDNAとcDNAの塩基配列情報の整備が必須であるので、第一の課題では、次世代シーケンサーを使って、ササウシノシタのゲノムDNAと全遺伝子のcDNAの塩基配列の解読を試みた。2番目に、ササウシノシタの雌親魚の腹腔に注射して、プラスミドを卵母細胞に導入できるか検討した。3番目に、ササウシノシタの成長ホルモン遺伝子(GH)を標的としたCRISPR/Cas9プラスミドを作成して、雌親魚の腹腔に注射し、GH遺伝子のノックアウトを試みた。

  12. Development of efficient delivery method for genome editing in aquaculture fish

    Suzuki Tohru

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2016/04/01 - 2019/03/31

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    To apply genome editing to aquaculture fish, this study aimed at development of delivery method of CRISPR/Cas9 into oocytes by injection into female abdominal cavity. It is known that yolk protein (Vg) is taken into the oocytes by using Vg receptor binding sequence, HLTKTKDL. We found that by binding with HLTKTKDL, exogenous protein injected into the female abdominal cavity is taken into the oocytes. However, since the protein was not transferred from yolk to embryonic cytoplasm, further improvement is required for applying this method to genome editing. We also found that when conjugate of plasmid vector and lipid carrier for in vivo usage was injected into female, protein is synthesized from injected vector in the oocytes. We expect that this method would enable the genome editing by injection into female.

  13. Isolation of genes expressed asymmetrically in the flounder metamorphosis and analysis of regulatory mechanisms by thyroid hormone(Fostering Joint International Research)

    Yokoi Hayato

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    Institution: Tohoku University

    2016 - 2019

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    Flatfish transform their body plan during metamorphosis. Flatfish are important fish as commercial target and actively produced by aquaculture, however, the abnormalities in pigmentation and malformation are the common issue around the world. In this international collaboration, we investigated the metamorphosis of flatfish comparing Japanese flounder and Mediterranean turbot. New findings include, blind-side specific degeneration of cartilage around eye was specific phenomena for flounder and not observed in turbot; genes involved in pigmentation is conserved among teleost fish but not in several tetrapods.

  14. Isolation of genes expressed asymmetrically in flounder metamorphosis and regulation by thyroid hormone

    Yokoi Hayato

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    Flounder transform their body plan during metamorphosis; right eye migrates to the other side and pigment cells differentiate only in the ocular side. Gene expression profile were compared between ocular and blind side in tissues around supra orbital bar at early metamorphic stage and skin and muscle tissue at later metamorphic stage in order to isolate genes expressed asymmetrically between ocular and blind side. Candidate genes were further analyzed by in situ hybridization and suggested that genes might be involved in right side specific degeneration of supra orbital bar and genes that genes might be involved in ocular side specific pigment cell differentiation.

  15. Approach to molecular system controlling left-right asymmetry of flatfish body color using next generation techniques

    SUZUKI Tohru

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    Flounder larvae are externally symmetric commonly to general fishes, but they transform to left-right (L-R) asymmetric body by migrating one eye to the other side of body and then differentiating chromatophores at ocular side skin. In the hatcheries for aquaculture, since abnormal pigmentation, including pigmentation of ocular side and pseudo-albinism of blind side, often appears, elucidation of the mechanism to induce abnormal pigmentation is required. This research revealed cellular and molecular systems that control L-R asymmetric skin color development in flounder, and also abnormal pigmentation

  16. Trial to identify genes related with fish characters using bioinformatics and genome database

    Suzuki Tohru, SAKAI Yoshifumi, YOKOI Hayato

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

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    One of representative genetic traits required for aquaculture is fish character adequate for breeding, such as non-nervousness. In mammals, polymorphism of single amino acid repeat and microsatellites in promoter region that evolved in the genes related with brain function cause variation of character among strains. Medaka strains of d-rR and HNI-II have opposing character; the former being tame, while the latter being nervous. Using these two strains, this study aimed at searching the genes correlated with characters of fish strains by using bioinformatics and genome databases. Serotonin receptor, androgen receptor and dopamine receptor were selected as candidate genes. Since polymorphism in repetitive sequences were detected among two strains and wild medaka, it is highly possible that selected three genes are actually correlated with character of strains.

  17. Molecular mechanisms of left-right asymmetric differentiation of flounder metamorphosis

    YOKOI Hayato

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    Flounder transform their body plan during metamorphosis and turn into asymmetric body form with two eyes on left side and ocular-side pigmentation. Metamorphosis is triggered by thyroid hormone signal, however, little is known about molecular basis of the metamorphic morphogenesis. Here, we compared gene expression profile between ocular and blind side by RNA-seq analysis using next generation sequencer, which identified 32 ocular side dominant genes and 7 blind side dominant genes. These candidates are now under screening by in situ hybridization to confirm left-right biased gene expression. So far, most of them showed biased gene expression as expected, which include known pigmentation genes such as gch2.

  18. Developmental control system of asymmetry of eye-sidedness and body coloration in flatfishes, and the mechanism that induces abnormality in the asymmetry development

    SUZUKI Tohru, YOKOI Hayato, UJI Susumu, FUJINAMI Yuichiro, SHIMIZU Daisuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2010/04/01 - 2014/03/31

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    We investigated the developmental mechanism that controls external asymmetry of flatfish (eye location and skin pigmentation). It was demonstrated that the Nodal signaling pathway (NSP), which is known to control the sidedness of internal organs and neural circuit of habenulae, fixes the eye-sidedness by controlling the forebrain asymmetry formation. The dextral and sinistral flatfishes are segregated by the opposite asymmetry formation at the habenulae and optic chiasma under the control of the NSP. It was found that the precursors of adult-type melanophores that locate at the base of dorsal fin migrate in the myoseptum toward both left and right lateral sides. The asymmetry of skin pigmentation is established by the maturation of precursors to chromatoblasts during the migration in the myoseptum of the ocular side. Exposure to retinoic acid (RA) induces maturation of the precursors also in the ocular side, resulting in the coloration of blind side skin.

  19. Development of fish iPS cells utilizing biological properties of fish

    SUZUKI Tohru, UJI Susumu, YOKOI Hayato

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2010 - 2012

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    Establishment of fish iPS cells is expected to enable the development of new techniques for maintaining selected aquaculture fish strains as frozen cells. This study aims as development of fundamental techniques essential for establishing fish iPS cells. We established medaka and zebrafish transgenic lines that emit GFP fluorescence under the control by oct4 promoter. We also developed cell culture system in which mulitpotency of cells can be monitored by GFP fluorescence. In addition, two possibilities on iPS cell production were suggested. First, transfection of Minicircle DNA may transform embryonic cells to iPS cells. Second, mulitpotent cells that appear in regenerating fin may be efficiently transformed to iPS cells.

  20. Molecular and developmental genetics of metamorphosis in fish

    YOKOI Hayato

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2010 - 2011

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    This study aimed to accumulate the basic scientific knowledge on the metamorphosis in fish to help establishing more efficient production of the flounder aquaculture which has a huge economical impact in Japan. I conducted molecular and developmental genetic studies using medaka as a model. GFP transgenic lines were generated in order to monitor in vivo thyroid hormone level in a live fish. In another approach to establish medaka as a model system for studying metamorphosis, approximately 20 candidate marker genes that are implicated in thyroid grand development and/or thyroid hormone signaling were cloned and their embryonic expression patterns were examined.

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Social Activities 19

  1. ゲノム広場

    2003/11/08 - 2003/11/09

  2. 京都大学 農学研究科 海洋生物増殖学特別セミナー

    2023/10/23 -

  3. 九州大学 農学研究院 国際農業教育研究・推進センター（CPIER）公開セミナー

    2023/03/10 -

  4. 岐阜大学 連合創薬医療情報研究科 Developmental Genetics Seminar

    2022/03/14 -

  5. バルセロナ大学遺伝学部門セミナー

    2018/06/27 -

  6. Keynote Lecture, University of Trento, Purple4 2nd Retreat

    University of Trento, Purple4 2nd Retreat

    2018/06/15 -

  7. 名古屋大学大学院 理学研究科 IGERセミナー

    2017/03/16 -

  8. バルセロナ大学遺伝学部門セミナー

    2016/07/11 -

  9. ハイデルベルグ大学マンハイム医学部セミナー

    2016/06/22 -

  10. 親子で楽しむ動物学１５

    2015/08/09 -

  11. オレゴン大学神経科学研究所セミナー

    2014/06/30 -

  12. 理研ライフサイエンス技術基盤研究センター

    2013/12/10 -

  13. 基礎生物学研究所 セミナー

    2009/06/04 -

  14. 東京工業大学大学院 生命理工学研究科 セミナー

    2009/06/03 -

  15. 東京大学大学院 理学系研究科 特別セミナー

    2009/06/02 -

  16. 東北大学大学院 生命科学研究科 GCOEセミナー

    2009/06/01 -

  17. 国立成育医療センター研究所 特別セミナー

    2009/05/27 -

  18. 国立遺伝学研究所 Biological Symposium

    2009/05/26 -

  19. 東海大学医学部 東海医学会セミナー

    2009/05/25 -

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Other 7

  1. 蒲生干潟および名取川河口域サンプリング

  2. 蒲生干潟および名取川河口域サンプリング

  3. 変態期ヒラメの左右非対称化プロセスの分子機構について

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    異体類ヒラメが左右対称な仔魚から左右非対称な成体の体制へと転換する変態について、眼球移動と左右非対称な色素細胞分化の分子基盤を明らかにする。

  4. 蒲生干潟および名取川河口域サンプリング

  5. ササウシノシタをモデルとしたヒラメ・カレイ類の変態機構に関する研究

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    ヒラメやナメタカレイ、ホシガレイなどは市場価値が高く、我が国では水産重要魚種として盛んに種苗が生産されているが、人工飼育下では変態不全仔魚が出現して問題となっている。本研究では小型のササウシノシタに着目し、異体類のモデルとして変態に関する基礎的データを蓄積して、大型の商業対象種に還元するシステムの構築を目指す。

  6. メダカをモデルとした魚類の変態に関する研究

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    モデル生物として実験環境が整備されているメダカの特性を活かして、魚類の変態過程を発生遺伝学的に解析する。具体的には、1. メダカにおける変態過程の記載、2. TILLING法による変態関連遺伝子の突然変異体解析を計画している。

  7. Eye migration and morphological transition during metamorphosis in sole fish

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    ヒラメ・カレイ類の変態のメカニズムを解明するために、小型で世代時間が短く遺伝学的な解析が可能なササウシノシタに着目して共同研究を行っている。ササウシノシタでは左眼が一日で右体側に移動することが知られており、経時観察により組織移動を詳細に解析する。

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