Details of the Researcher

PHOTO

Asako Sugimoto
Section
Board Member
Job title
Executive Vice President
Degree

  • 博士（理学）（東京大学）

  • 理学修士（東京大学）

Research History 11

  • 2024/04 - Present
    Tohoku University　Executive Vice President for Research

  • 2010/04 - Present
    Tohoku University　Graduate School of Life Sciences　Professor

  • 2022/04 - 2023/03
    Tohoku University

  • 2019/04 - 2023/03
    Tohoku University　Graduate School of Life Sciences　Dean

  • 2009/05 - 2011/03
    大阪大学大学院理学系研究科生物科学専攻　招へい教授（併任）

  • 2001/02 - 2011/03
    独立行政法人 理化学研究所 発生・再生科学総合研究センター 発生ゲノミクス研究チーム　チームリーダー

  • 2006/04 - 2009/04
    大阪大学大学院理学系研究科生物科学専攻　招へい准教授（併任）

  • 2002/04 - 2006/03
    神戸大学大学院医学系研究科医学専攻 生命医学科学領域　客員助教授（併任）

  • 1996/04 - 2002/03
    Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo　Research Associate

  • 1997/10 - 2000/09
    科学技術振興事業団さきがけ研究21「素過程と連携」　研究者

  • 1992/04 - 1996/03
    Department of Biochemistry, University of Wisconsin-Madison　postdoctoral fellow

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Education 2

  • The University of Tokyo　Graduate School, Division of Science　生物化学専攻

    - 1992/03

  • The University of Tokyo　Faculty of Science　生物化学科

    - 1987/03

Committee Memberships 49

  • 国立遺伝学研究所　生物遺伝資源委員会委員

    2022/08 - Present

  • 日本細胞生物学会　常任編集委員

    2017/01 - Present

  • 日本細胞生物学会　代議員

    2014/06 - Present

  • 日本分子生物学会　Genes to Cells編集委員

    2014/04 - Present

  • Genes to Cells　Associate Editor

    2010/04 - Present

  • 日本学術会議　連携会員

    2023/10 - 2030/09

  • 大阪大学 蛋白質研究所　運営協議会委員

    2022/04 - 2027/03

  • 科学技術振興機構　CRDS第2バイオ分野別委員会

    2022/09 - 2026/03

  • 文部科学省 科学技術・学術審議会 研究計画・評価分科会 ライフサイエンス委員会　科学技術・学術審議会専門委員

    2023/05 - 2025/02

  • 日本分子生物学会　理事

    2023/01 - 2024/12

  • 科学技術振興機構　選考・推進パネルオフィサー

    2022/10 - 2024/09

  • 河北新報社　読者と考える紙面委員会委員

    2019/11 - 2024/04

  • Molecular Biology of the Cell　Associate Editor

    2020/01 - 2023/12

  • 日本学術会議　会員

    2017/10 - 2023/09

  • 国立大学協会　ファシリテーター

    2020/05 - 2023/03

  • 東北大学出版会　評議員

    2019/04 - 2023/03

  • Molecular Biology Society of Japan　Vice President

    2021/01 - 2022/12

  • The International C. elegans Board (WormBoard)　Interim Regional Representative (Asia/Australasia/Oceania)

    2015/11 - 2021/06

  • 自然科学研究機構 基礎生物学研究所　運営会議委員

    2017/04 - 2021/03

  • 公益財団法人加藤記念バイオサイエンス振興財団　加藤記念研究助成選考委員

    2016/04 - 2020/03

  • 情報・システム研究機構国立遺伝学研究所　運営会議委員

    2013/04 - 2019/03

  • 科学技術振興機構 戦略的想像研究推進事業（さきがけ）「細胞機能の構成的な理解と制御」領域　アドバイザー

    2012/08 - 2019/03

  • 日本医療研究開発機構　アドバイザリーボード委員

    2017/03 - 2018/12

  • 日本分子生物学会　理事

    2017/01 - 2018/12

  • 日本分子生物学会　第20期 理事長

    2017/01 - 2018/12

  • 日本細胞生物学会　理事・CSF編集委員

    2016/06 - 2018/06

  • 日本学術振興会　科学研究費委員会専門委員

    2016/12 - 2017/11

  • 日本学術会議　発生生物学分科会委員

    2015/07 - 2017/09

  • 日本学術会議　連携会員

    2011/10 - 2017/09

  • 日本分子生物学会　理事・国際会議支援選考委員・研究倫理委員

    2015/01 - 2016/12

  • 文部科学省　科学研究費補助金における評価に関する委員会の評価者

    2015/12 - 2016/11

  • 日本学術振興会　特別研究員等審査会専門委員および国際事業委員会書面審査員

    2015/08 - 2016/07

  • 公益財団法人 稲盛財団　京都賞基礎科学部門専門委員会委員

    2015/07 - 2016/06

  • 日本細胞生物学会　理事・CSF編集委員

    2014/09 - 2016/03

  • 日本細胞生物学会　運営委員

    2012/04 - 2016/03

  • Developmental Dynamics　Editorial Board

    2003/04 - 2016/03

  • 文部科学省　科学研究費補助金における評価に関する委員会の評価者

    2014/12 - 2015/11

  • 日本学術振興会　特別研究員等審査会専門委員および国際事業委員会書面審査員

    2014/08 - 2015/07

  • 文部科学省　科学研究費補助金における評価に関する委員会の評価者

    2013/12 - 2014/11

  • 日本学術会議　分子生物学分科会

    2011/11 - 2014/09

  • 日本学術振興会　特別研究員等審査会専門委員および国際事業委員会書面審査員

    2013/08 - 2014/07

  • 日本分子生物学会　理事

    2011/04 - 2012/03

  • 科学技術振興機構 戦略的想像研究推進事業（さきがけ）「RNAと生体機能」領域　アドバイザー

    2007/04 - 2012/03

  • 日本分子生物学会　理事・男女共同参画委員長

    2008/04 - 2010/03

  • 日本分子生物学会　会計幹事

    2003/04 - 2005/03

  • C. elegans Development, Cell Biology and Gene Expression Meeting　Organizer

    2014/07 -

  • 日本細胞生物学会　第63回年会（平成23）プログラム委員

    2011/04 -

  • 日本分子生物学会　第34回年会（平成23）プログラム委員

    2011/04 -

  • 日本分子生物学会　第25回年会（平成13）プログラム委員

    2001/04 -

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Professional Memberships 2

  • 日本細胞生物学会

  • 日本分子生物学会

Research Interests 9

  • ゲノム機能解析

  • germ cell

  • microtubule

  • 細胞骨格

  • cell fate

  • cell polarity

  • cell division

  • embryogenesis

  • C. elegans

Research Areas 4

  • Life sciences / Genomics /

  • Life sciences / Developmental biology /

  • Life sciences / Cell biology /

  • Life sciences / Molecular biology /

Papers 69

  1. Biology and genome of a newly discovered sibling species of Caenorhabditis elegans. International-journal Peer-reviewed

    Natsumi Kanzaki, Isheng J Tsai, Ryusei Tanaka, Vicky L Hunt, Dang Liu, Kenji Tsuyama, Yasunobu Maeda, Satoshi Namai, Ryohei Kumagai, Alan Tracey, Nancy Holroyd, Stephen R Doyle, Gavin C Woodruff, Kazunori Murase, Hiromi Kitazume, Cynthia Chai, Allison Akagi, Oishika Panda, Huei-Mien Ke, Frank C Schroeder, John Wang, Matthew Berriman, Paul W Sternberg, Asako Sugimoto, Taisei Kikuchi

    Nature communications　9　(1)　3216-3216　2018/08/10

    DOI： 10.1038/s41467-018-05712-5 　

  2. SLC-25A46 regulates mitochondrial fusion through FZO-1/Mitofusin and is. International-journal

    Hiroyuki Obinata, Taisei Watanabe, Hironori Takahashi, Satoshi Shimo, Toshiyuki Oda, Asako Sugimoto, Shinsuke Niwa

    Journal of cell science　2025/05/30

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1242/jcs.263571 　

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    Abstract Mitochondria are dynamic organelles shaped by sequential fission and fusion events. The mitochondrial protein SLC25A46 has been identified as a causative gene for mitochondrial neuropathies. However, the function of SLC25A46 in mitochondrial morphogenesis remains controversial, with several reports suggesting it acts as a mitochondrial fission factor, while others propose it as a fusion factor. In this study, employing forward genetics, we identifiedslc-25A46, aCaenorhabditis elegansorthologue of human SLC25A46, as an essential factor for mitochondrial fusion. Suppressor mutagenesis screening revealed loss-of-function mutations indrp-1, a mitochondrial fission factor, as suppressors ofslc-25A46. The phenotype ofslc-25A46mutants is similar to those offzo-1mutants, wherein the mitochondrial fusion factor Mitofusin is disrupted. Overexpressing FZO-1/Mitofusin mitigated mitochondrial defects inslc-25a46mutants, indicating SLC-25A46 promotes fusion through FZO-1/Mitofusin. Disease model worms carrying mutations associated with SLC25A46 exhibited mitochondrial fragmentation and accelerated neurodegeneration, suggestingslc-25A46maintains neuronal morphology through mitochondrial fusion regulation.

  3. Microparticle Bombardment as a Method for Transgenesis in Auanema and Tokorhabditis. International-journal

    Tatsuya Yamashita, Andre Pires-daSilva, Shun Oomura, Taichi Kusano, Nami Haruta, Mayu Hasumi, Taisei Kikuchi, Sally Adams, Asako Sugimoto, Ryoji Shinya

    microPublication biology　2025　2025

    DOI： 10.17912/micropub.biology.001585 　

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    Functional gene analysis tools in Caenorhabditis elegans are often ineffective in other nematodes due to differences in gonadal morphology and transgene silencing. Here, we established a method to generate stable transgenic lines in the nematodes Auanema freiburgense and Tokorhabditis tufae using microparticle bombardment coupled with hygromycin B selection. Despite using non-codon-optimized GFP, transgenic strains expressing fluorescent markers were obtained in both species. Additionally, an Auanema codon-optimized RFP construct showed robust expression in all tissues. This method will be valuable for future studies into the unusual sex determination, viviparity, and stress resistance in Auanema and Tokorhabditis .

  4. Dramatic evolutionary changes of conserved noncoding elements accompanied by extraordinary features ofCaenorhabditis inopinata

    Katsunori Tamagawa, Shun Oomura, Asako Sugimoto, Takashi Makino

    2024/09/21

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1101/2024.09.18.613604 　

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    Phenotypic evolution is caused by mutations in noncoding and protein-coding regions. Conserved noncoding elements (CNEs) have-at least partially-gene regulatory functions and contribute to the evolution of organisms by modifying their gene expression. While evolutionary changes of CNEs-such as loss and accelerated evolution-have been intensively studied in vertebrates, only a few studies have focused on invertebrates. The availability of detailed functional genomic information of the model nematodeCaenorhabditis elegansand recent accumulation of genome sequences within the same genus facilitate the use of these nematodes in comparative genomics focusing on CNEs. The recently discovered speciesCaenorhabditis inopinatahas several peculiar traits characterized by a larger body size than that of its close relatives and are regarded as an appropriate species that links phenotypic evolution with genomic changes. Herein, using comparative genomics and transcriptomics inC. inopinata, we analyzed the evolution of CNEs and their functions during phenotypic evolution. We detected substantial evolutionary changes in CNEs inC. inopinatacompared to other relatives-changes frequently associated with body morphology and behavior corresponding to extraordinary body size and unique life cycles. Our study revealed an association between noncoding elements and substantial phenotypic evolution in invertebrates.

  5. Evolutionary changes of noncoding elements associated with transition of sexual mode in Caenorhabditis nematodes

    Katsunori Tamagawa, Mehmet Dayi, Simo Sun, Rikako Hata, Taisei Kikuchi, Nami Haruta, Asako Sugimoto, Takashi Makino

    Science Advances　10　(37)　2024/09/13

    Publisher: American Association for the Advancement of Science (AAAS)

    DOI： 10.1126/sciadv.adn9913 　

    eISSN： 2375-2548

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    The transition of the sexual mode occurs widely in animal evolution. In Caenorhabditis nematodes, androdioecy, a sexual polymorphism composed of males and hermaphrodites having the ability to self-fertilize, has evolved independently multiple times. While the modification of noncoding regulatory elements likely contributed to the evolution of hermaphroditism, little is known about these changes. Here, we conducted a genome-wide analysis of conserved noncoding elements (CNEs) focusing on the evolution of hermaphroditism in Caenorhabditis nematodes. We found that, in androdioecious nematodes, mutations rapidly accumulated in CNEs’ neighboring genes associated with sexual traits. Expression analysis indicate that the identified CNEs are involved in spermatogenesis in hermaphrodites and associated with the transition of gene expression from dioecious to androdioecious nematodes. Last, genome editing of a CNE neighboring laf-1 resulted in a change in its expression in the gonadal region undergoing spermatogenesis. Our bioinformatic and experimental analyses highlight the importance of CNEs in gene regulation associated with the development of hermaphrodites.

  6. The impact of differential transposition activities of autonomous and non-autonomous hAT transposable elements on genome architecture and gene expression in Caenorhabditis inopinata

    Ryuhei Hatanaka, Katsunori Tamagawa, Nami Haruta, Asako Sugimoto

    GENETICS　2024/04/05

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/genetics/iyae052 　

    eISSN： 1943-2631

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    Abstract Transposable elements (TEs) are DNA sequences capable of moving within genomes and significantly influence genomic evolution. The nematode Caenorhabditis inopinata exhibits a much higher TE copy number than its sister species, C. elegans. In this study, we identified a novel autonomous TE belonging to the hAT superfamily from a spontaneous TE-insertion mutant in C. inopinata and named this transposon Ci-hAT1. Further bioinformatic analyses uncovered three additional autonomous hAT elements—Ci-hAT2, Ci-hAT3, and Ci-hAT4—along with over 1,000 copies of two non-autonomous miniature inverted-repeat transposable elements (MITEs), mCi-hAT1 and mCi-hAT4, likely derived from Ci-hAT1 and Ci-hAT4 through internal deletion. We tracked at least three sequential transpositions of Ci-hAT1 over several years. However, the transposition rates of the other three autonomous hAT elements were lower, suggesting varying activity levels. Notably, the distribution patterns of the two MITE families differed significantly: mCi-hAT1 was primarily located in the chromosome arms, a pattern observed in the TEs of other Caenorhabditis species, whereas mCi-hAT4 was more evenly distributed across chromosomes. Additionally, interspecific transcriptome analysis indicated that C. inopinata genes with upstream or intronic these MITE insertions tend to be more highly expressed than their orthologous genes in C. elegans. These findings highlight the significant role of de-silenced TEs in driving the evolution of genomes and transcriptomes, leading to species-specific genetic diversity.

  7. Syntenic relationship of chromosomes in Strongyloides species and Rhabditophanes diutinus based on the chromosome-level genome assemblies

    Asuka Kounosu, Simo Sun, Yasunobu Maeda, Mehmet Dayi, Akemi Yoshida, Haruhiko Maruyama, Vicky Hunt, Asako Sugimoto, Taisei Kikuchi

    Philosophical Transactions of the Royal Society B: Biological Sciences　2024/01/15

    DOI： 10.1098/rstb.2022.0446 　

    ISSN： 0962-8436 1471-2970

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    <jats:p> The <jats:italic>Strongyloides</jats:italic> clade, to which the parasitic nematode genus <jats:italic>Strongyloides</jats:italic> belongs, contains taxa with diverse lifestyles, ranging from free-living to obligate vertebrate parasites. Reproductive strategies are also diverse in this group of nematodes, employing not only sexual reproduction but also parthenogenesis, making it an attractive group to study genome adaptation to specific conditions. An in-depth understanding of genome evolution, however, has been hampered by fragmented genome assemblies. In this study, we generated chromosome-level genome assemblies for two <jats:italic>Strongyloides</jats:italic> species and the outgroup species <jats:italic>Rhabditophanes diutinus</jats:italic> using long-read sequencing and high‐throughput chromosome conformation capture (Hi-C). Our synteny analyses revealed a clearer picture of chromosome evolution in this group, suggesting that a functional sex chromosome has been maintained throughout the group. We further investigated sex chromosome dynamics in the lifecycle of <jats:italic>Strongyloides ratti</jats:italic> and found that bivalent formation in oocytes appears to be important for male production in the mitotic parthenogenesis. </jats:p> <jats:p> This article is part of the Theo Murphy meeting issue ‘ <jats:italic>Strongyloides</jats:italic> : omics to worm-free populations’. </jats:p>

  8. A germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans. International-journal

    Nami Haruta, Eisuke Sumiyoshi, Yu Honda, Masahiro Terasawa, Chihiro Uchiyama, Mika Toya, Yukihiko Kubota, Asako Sugimoto

    Journal of cell science　136　(13)　2023/07/01

    DOI： 10.1242/jcs.260922 　

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    The γ-tubulin complex (γTuC) is a widely conserved microtubule nucleator, but some of its components, namely GCP4, GCP5 and GCP6 (also known as TUBGCP4, TUBGCP5 and TUBGCP6, respectively), have not been detected in Caenorhabditis elegans. Here, we identified two γTuC-associated proteins in C. elegans, GTAP-1 and GTAP-2, for which apparent orthologs were detected only in the genus Caenorhabditis. GTAP-1 and GTAP-2 were found to localize at centrosomes and the plasma membrane of the germline, and their centrosomal localization was interdependent. In early C. elegans embryos, whereas the conserved γTuC component MZT-1 (also known as MOZART1 and MZT1) was essential for the localization of centrosomal γ-tubulin, depletion of GTAP-1 and/or GTAP-2 caused up to 50% reduction of centrosomal γ-tubulin and precocious disassembly of spindle poles during mitotic telophase. In the adult germline, GTAP-1 and GTAP-2 contributed to efficient recruitment of the γTuC to the plasma membrane. Depletion of GTAP-1, but not of GTAP-2, severely disrupted both the microtubule array and the honeycomb-like structure of the adult germline. We propose that GTAP-1 and GTAP-2 are unconventional components of the γTuC that contribute to the organization of both centrosomal and non-centrosomal microtubules by targeting the γTuC to specific subcellular sites in a tissue-specific manner.

  9. Differentially expressed genes associated with body size changes and transposable element insertions between Caenorhabditis elegans and its sister species, C. inopinata.

    Kazuma Kawahara, Taruho Inada, Ryusei Tanaka, Mehmet Dayi, Takashi Makino, Shinichi Maruyama, Taisei Kikuchi, Asako Sugimoto, Masakado Kawata

    Genome Biology and Evolution　2023/04/18

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/gbe/evad063 　

    eISSN： 1759-6653

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    Abstract Why the recently discovered nematode Caenorhabditis inopinata differs so greatly from its sibling species Caenorhabditis elegans remains unknown. A previous study showed that C. inopinata has more transposable elements (TEs), sequences that replicate and move autonomously throughout the genome, potentially altering the expression of neighboring genes. In this study, we focused on how the body size of this species has evolved and whether TEs could affect the expression of genes related to species-specific traits such as body size. First, we compared gene expression levels between C. inopinata and C. elegans in the L4 larval and young adult stages—when growth rates differ most prominently between these species—to identify candidate genes contributing to their differences. The results showed that the expression levels of collagen genes were consistently higher in C. inopinata than in C. elegans and that some genes related to cell size were differentially expressed between the species. Then, we examined whether genes with TE insertions are differentially expressed between species. Indeed, the genes featuring C. inopinata-specific TE insertions had higher expression levels in C. inopinata than in C. elegans. These upregulated genes included those related to body size, suggesting that these genes could be candidates for artificial TE insertion to examine the role of TEs in the body size evolution of C. inopinata.

  10. C. elegans ATG-5 mutants associated with ataxia. International-journal

    Azusa Yugeta, Hiroki Arai, Daiki Takahashi, Nami Haruta, Asako Sugimoto, Hirokazu Arimoto

    microPublication biology　2023　2023

    DOI： 10.17912/micropub.biology.000792 　

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    Intercellular cleaning via autophagy is crucial for maintaining cellular homeostasis, and impaired autophagy has been associated with the accumulation of protein aggregates that can contribute to neurological diseases. Specifically, the loss-of-function mutation in the human autophagy-related gene 5 (ATG5) at E122D has been linked to the pathogenesis of spinocerebellar ataxia in humans. In this study, we generated two homozygous C. elegans strains with mutations (E121D and E121A) at positions corresponding to the human ATG5 ataxia mutation to investigate the effects of ATG5 mutations on autophagy and motility. Our results showed that both mutants exhibited a reduction in autophagy activity and impaired motility, suggesting that the conserved mechanism of autophagy-mediated regulation of motility extends from C. elegans to humans.

  11. The PAF1 complex cell autonomously promotes oogenesis in Caenorhabditis elegans International-journal Peer-reviewed

    Yukihiro Kubota, Natsumi Ota, Hisashi Takatsuka, Takuma Unno, Shuichi Onami, Asako Sugimoto, Masahiro Ito

    Genes to Cells　2022/04/27

    Publisher: Wiley

    DOI： 10.1111/gtc.12938 　

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    The RNA polymerase II-associated factor 1 complex (PAF1C) is a protein complex that consists of LEO1, RTF1, PAF1, CDC73, and CTR9, and has been shown to be involved in RNA polymerase II-mediated transcriptional and chromatin regulation. Although it has been shown to regulate a variety of biological processes, the precise role of the PAF1C during germ line development has not been clarified. In this study, we found that reduction in the function of the PAF1C components, LEO-1, RTFO-1, PAFO-1, CDC-73, and CTR-9, in Caenorhabditis elegans affects oogenesis. Defects in oogenesis were also confirmed using an oocyte maturation marker, OMA-1::GFP. While four to five OMA-1::GFP-positive oocytes were observed in wild-type animals, their numbers were significantly decreased in pafo-1 mutant and leo-1(RNAi), pafo-1(RNAi), and cdc-73(RNAi) animals. Expression of a functional PAFO-1::mCherry transgene in the germline significantly rescued the oogenesis-defective phenotype of the pafo-1 mutants, suggesting that expression of the PAF1C in germ cells is required for oogenesis. Notably, overexpression of OMA-1::GFP partially rescued the oogenesis defect in the pafo-1 mutants. Based on our findings, we propose that the PAF1C promotes oogenesis in a cell-autonomous manner by positively regulating the expression of genes involved in oocyte maturation.

  12. Centrosome maturation requires phosphorylation-mediated sequential domain interactions of SPD-5 International-journal Peer-reviewed

    Momoe Nakajo, Hikaru Kano, Kenji Tsuyama, Nami Haruta, Asako Sugimoto

    Journal of Cell Science　135　(8)　2022/04/15

    Publisher: The Company of Biologists

    DOI： 10.1242/jcs.259025 　

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    Centrosomes consist of two centrioles and the surrounding pericentriolar material (PCM). The PCM expands during mitosis in a process called centrosome maturation, in which PCM scaffold proteins play pivotal roles to recruit other centrosomal proteins. In Caenorhabditis elegans, the scaffold protein SPD-5 forms a PCM scaffold in a polo-like kinase 1 (PLK-1) phosphorylation-dependent manner. However, how phosphorylation of SPD-5 promotes PCM scaffold assembly is unclear. Here, we identified three functional domains of SPD-5 through in vivo domain analyses, and propose that sequential domain interactions of SPD-5 are required for mitotic PCM scaffold assembly. Firstly, SPD-5 is targeted to centrioles through a direct interaction between its centriole localization (CL) domain and the centriolar protein PCMD-1. Then, intramolecular and intermolecular interactions between the SPD-5 phospho-regulated multimerization (PReM) domain and the PReM association (PA) domain are enhanced by phosphorylation by PLK-1, which leads to PCM scaffold expansion. Our findings suggest that the sequential domain interactions of scaffold proteins mediated by PLK-1 phosphorylation is an evolutionarily conserved mechanism of PCM scaffold assembly. This article has an associated First Person interview with the first author of the paper.

  13. The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans International-journal Peer-reviewed

    Takefumi Negishi, Saho Kitagawa, Natsumi Horii, Yuka Tanaka, Nami Haruta, Asako Sugimoto, Hitoshi Sawa, Ken-ichiro Hayashi, Masahiko Harata, Masato T Kanemaki

    Genetics　220　(2)　2022/02/04

    Publisher: Oxford University Press ({OUP})

    DOI： 10.1093/genetics/iyab218 　

    ISSN： 1943-2631

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    <jats:title>Abstract</jats:title> <jats:p>Targeted protein degradation using the auxin-inducible degron (AID) system is garnering attention in the research field of Caenorhabditis elegans, because of the rapid and efficient target depletion it affords, which can be controlled by treating the animals with the phytohormone auxin. However, the current AID system has drawbacks, i.e., leaky degradation in the absence of auxin and the requirement for high auxin doses. Furthermore, it is challenging to deplete degron-fused proteins in embryos because of their eggshell, which blocks auxin permeability. Here, we apply an improved AID2 system utilizing AtTIR1(F79G) and 5-phenyl-indole-3-acetic acid (5-Ph-IAA) to C. elegans and demonstrated that it confers better degradation control vs the previous system by suppressing leaky degradation and inducing sharp degradation using 1,300-fold lower 5-Ph-IAA doses. We successfully degraded the endogenous histone H2A.Z protein fused to an mAID degron and disclosed its requirement in larval growth and reproduction, regardless of the presence of maternally inherited H2A.Z molecules. Moreover, we developed an eggshell-permeable 5-Ph-IAA analog, 5-Ph-IAA-AM, that affords an enhanced degradation in laid embryos. Our improved system will contribute to the disclosure of the roles of proteins in C. elegans, in particular those that are involved in embryogenesis and development, through temporally controlled protein degradation.</jats:p>

  14. Transgenesis of the gonochoristic nematode Caenorhabditis inopinata by microparticle bombardment with hygromycin B selection. International-journal

    Shun Oomura, Kenji Tsuyama, Nami Haruta, Asako Sugimoto

    microPublication biology　2022　2022

    DOI： 10.17912/micropub.biology.000564 　

  15. Expression Patterns and Levels of All Tubulin Isotypes Analyzed in GFP Knock-In C. elegans Strains. Peer-reviewed

    Kei Nishida, Kenta Tsuchiya, Hiroyuki Obinata, Shizuka Onodera, Yu Honda, Yen-Cheng Lai, Nami Haruta, Asako Sugimoto

    Cell Structure and Function　46　(1)　51-64　2021/06/30

    DOI： 10.1247/csf.21022 　

    More details Close

    Most organisms have multiple α- and β-tubulin isotypes that likely contribute to the diversity of microtubule (MT) functions. To understand the functional differences of tubulin isotypes in Caenorhabditis elegans, which has nine α-tubulin isotypes and six β-tubulin isotypes, we systematically constructed null mutants and GFP-fusion strains for all tubulin isotypes with the CRISPR/Cas9 system and analyzed their expression patterns and levels in adult hermaphrodites. Four isotypes-α-tubulins TBA-1 and TBA-2 and β-tubulins TBB-1 and TBB-2-were expressed in virtually all tissues, with a distinct tissue-specific spectrum. Other isotypes were expressed in specific tissues or cell types at significantly lower levels than the broadly expressed isotypes. Four isotypes (TBA-5, TBA-6, TBA-9, and TBB-4) were expressed in different subsets of ciliated sensory neurons, and TBB-4 was inefficiently incorporated into mitotic spindle MTs. Taken together, we propose that MTs in C. elegans are mainly composed of four broadly expressed tubulin isotypes and that incorporation of a small amount of tissue-specific isotypes may contribute to tissue-specific MT properties. These newly constructed strains will be useful for further elucidating the distinct roles of tubulin isotypes.Key words: tubulin isotypes, microtubules, C. elegans.

  16. Fluorescence-labeled neopeltolide derivatives for subcellular localization imaging. International-journal Peer-reviewed

    Yanagi Shota, Sugai Tomoya, Noguchi Takuma, Kawakami Masao, Sasaki Makoto, Niwa Shinsuke, Sugimoto Asako, Fuwa Haruhiko

    Organic & Biomolecular Chemistry　17　(28)　6771-6776　2019/08

    DOI： 10.1039/c9ob01276a 　

    More details Close

    Design, synthesis and functional analysis of fluorescent derivatives of neopeltolide, an antiproliferative marine macrolide, are reported herein. Live cell imaging using the fluorescent derivatives showed rapid cellular uptake and localization within the endoplasmic reticulum as well as the mitochondria.

  17. The Role of Tissue Inhibitors of Metalloproteinases in Organ Development and Regulation of ADAMTS Family Metalloproteinases in Caenorhabditis elegans. International-journal Peer-reviewed

    Yukihiko Kubota, Kiyoji Nishiwaki, Masahiro Ito, Asako Sugimoto

    Genetics　212　(2)　523-535　2019/06

    DOI： 10.1534/genetics.119.301795 　

    More details Close

    Remodeling of the extracellular matrix supports tissue and organ development, by regulating cellular morphology and tissue integrity. However, proper extracellular matrix remodeling requires spatiotemporal regulation of extracellular metalloproteinase activity. Members of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family, including MIG-17 and GON-1, are evolutionarily conserved, secreted, zinc-requiring metalloproteinases. Although these proteases are required for extracellular matrix remodeling during gonadogenesis in Caenorhabditis elegans, their in vivo regulatory mechanisms remain to be delineated. Therefore, we focused on the C. elegans tissue inhibitors of metalloproteinases (TIMPs), TIMP-1 and CRI-2 Analysis of the transcription and translation products for GFP/Venus fusions, with TIMP-1 or CRI-2, indicated that these inhibitors were secreted and localized to the basement membrane of gonads and the plasma membrane of germ cells. A timp-1 deletion mutant exhibited gonadal growth defects and sterility, and the phenotypes of this mutant were fully rescued by a TIMP-1::Venus construct, but not by a TIMP-1(C21S)::Venus mutant construct, in which the inhibitor coding sequence had been mutated. Moreover, genetic data suggested that TIMP-1 negatively regulates proteolysis of the α1 chain of type IV collagen. We also found that the loss-of-function observed for the mutants timp-1 and cri-2 involves a partial suppression of gonadal defects found for the mutants mig-17/ADAMTS and gon-1/ADAMTS, and that this suppression was canceled upon overexpression of gon-1 or mig-17, respectively. Based on these results, we propose that both TIMP-1 and CRI-2 act as inhibitors of MIG-17 and GON-1 ADAMTSs to regulate gonad development in a noncell-autonomous manner.

  18. Streptothricin acetyl transferase 2 (Sat2): A dominant selection marker for Caenorhabditis elegans genome editing Peer-reviewed

    Hiroyuki Obinata, Asako Sugimoto, Shinsuke Niwa

    PLoS ONE　13　(5)　2018/05/01

    Publisher: Public Library of Science

    DOI： 10.1371/journal.pone.0197128 　

    ISSN： 1932-6203

  19. Transgenesis by microparticle bombardment for live imaging of fluorescent proteins in Pristionchus pacificus germline and early embryos Peer-reviewed

    Satoshi Namai, Asako Sugimoto

    Development Genes and Evolution　228　(1)　75-82　2018/01/01

    Publisher: Springer Verlag

    DOI： 10.1007/s00427-018-0605-z 　

    ISSN： 1432-041X 0949-944X

  20. Tubulin isotype substitution revealed that isotype combination modulates microtubule dynamics in C. elegans embryos Peer-reviewed

    Yu Honda, Kenta Tsuchiya, Eisuke Sumiyoshi, Nami Haruta, Asako Sugimoto

    JOURNAL OF CELL SCIENCE　130　(9)　1652-1661　2017/05

    DOI： 10.1242/jcs.200923 　

    ISSN： 0021-9533

    eISSN： 1477-9137

  21. Caenorhabditis elegans Aurora A kinase is required for the formation of spindle microtubules in female meiosis Peer-reviewed

    Eisuke Sumiyoshi, Yuma Fukata, Satoshi Namai, Asako Sugimoto

    MOLECULAR BIOLOGY OF THE CELL　26　(23)　4187-4196　2015/11

    DOI： 10.1091/mbc.E15-05-0258 　

    ISSN： 1059-1524

    eISSN： 1939-4586

  22. Protein Phosphatase 4 Promotes Chromosome Pairing and Synapsis, and Contributes to Maintaining Crossover Competence with Increasing Age Peer-reviewed

    Aya Sato-Carlton, Xuan Li, Oliver Crawley, Sarah Testori, Enrique Martinez-Perez, Asako Sugimoto, Peter M. Carlton

    PLOS GENETICS　10　(10)　e1004638-e1004638　2014/10

    DOI： 10.1371/journal.pgen.1004638 　

    ISSN： 1553-7390

    eISSN： 1553-7404

  23. The PAF1 complex is involved in embryonic epidermal morphogenesis in Caenorhabditis elegans Peer-reviewed

    Yukihiko Kubota, Kenji Tsuyama, Yusuke Takabayashi, Nami Haruta, Rika Maruyama, Naoko Iida, Asako Sugimoto

    DEVELOPMENTAL BIOLOGY　391　(1)　43-53　2014/07

    DOI： 10.1016/j.ydbio.2014.04.002 　

    ISSN： 0012-1606

    eISSN： 1095-564X

  24. The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A Peer-reviewed

    Elsa Kress, Francoise Schwager, Rene Holtackers, Jonas Seiler, Francois Prodon, Esther Zanin, Annika Eiteneuer, Mika Toya, Asako Sugimoto, Hemmo Meyer, Patrick Meraldi, Monica Gotta

    JOURNAL OF CELL BIOLOGY　201　(4)　559-575　2013/05

    DOI： 10.1083/jcb.201209107 　

    ISSN： 0021-9525

  25. The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process in Caenorhabditis elegans embryos Peer-reviewed

    Virginie Hachet, Coralie Busso, Mika Toya, Asako Sugimoto, Peter Askjaer, Pierre Goenczy

    MOLECULAR BIOLOGY OF THE CELL　23　(16)　3111-3121　2012/08

    DOI： 10.1091/mbc.E12-03-0204 　

    ISSN： 1059-1524

  26. Tissue Architecture in the Caenorhabditis elegans Gonad Depends on Interactions Among Fibulin-1, Type IV Collagen and the ADAMTS Extracellular Protease Peer-reviewed

    Yukihiko Kubota, Kayo Nagata, Asako Sugimoto, Kiyoji Nishiwaki

    GENETICS　190　(4)　1379-+　2012/04

    DOI： 10.1534/genetics.111.133173 　

    ISSN： 0016-6731

  27. Cell Polarity: Centrosomes Release Signals for Polarization Invited Peer-reviewed

    Eisuke Sumiyoshi, Asako Sugimoto

    CURRENT BIOLOGY　22　(8)　R281-R283　2012/04

    DOI： 10.1016/j.cub.2012.03.009 　

    ISSN： 0960-9822

  28. Toward the second stage of recovery from the 3.11 Tohoku Earthquake Invited Peer-reviewed

    Asako Sugimoto

    GENES TO CELLS　16　(7)　745-747　2011/07

    DOI： 10.1111/j.1365-2443.2011.01531.x 　

    ISSN： 1356-9597

  29. The beta-catenin HMP-2 functions downstream of Src in parallel with the Wnt pathway in early embryogenesis of C. elegans Peer-reviewed

    Eisuke Sumiyoshi, Sachiko Takahashi, Hatsue Obata, Asako Sugimoto, Yuji Kohara

    DEVELOPMENTAL BIOLOGY　355　(2)　302-312　2011/07

    DOI： 10.1016/j.ydbio.2011.04.034 　

    ISSN： 0012-1606

  30. A kinase-independent role for Aurora A in the assembly of mitotic spindle microtubules in Caenorhabditis elegans embryos Peer-reviewed

    Mika Toya, Masahiro Terasawa, Kayo Nagata, Yumi Tida, Asako Sugimoto

    NATURE CELL BIOLOGY　13　(6)　708-U187　2011/06

    DOI： 10.1038/ncb2242 　

    ISSN： 1465-7392

  31. PGL proteins self associate and bind RNPs to mediate germ granule assembly in C. elegans Peer-reviewed

    Momoyo Hanazawa, Masafumi Yonetani, Asako Sugimoto

    JOURNAL OF CELL BIOLOGY　192　(6)　929-937　2011/03

    DOI： 10.1083/jcb.201010106 　

    ISSN： 0021-9525

  32. Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150Glued and DNC-2/dynamitin Peer-reviewed

    Masahiro Terasawa, Mika Toya, Fumio Motegi, Miyeko Mana, Kuniaki Nakamura, Asako Sugimoto

    GENES TO CELLS　15　(11)　1145-1157　2010/11

    DOI： 10.1111/j.1365-2443.2010.01451.x 　

    ISSN： 1356-9597

  33. Imaging of Mitotic Spindle Dynamics in Caenorhabditis elegans Embryos Invited Peer-reviewed

    Mika Toya, Yumi Iida, Asako Sugimoto

    MICROTUBULES: IN VIVO　97　359-372　2010

    DOI： 10.1016/S0091-679X(10)97019-2 　

    ISSN： 0091-679X

  34. The Role of Protein Phosphatase 4 in Regulating Microtubule Severing in the Caenorhabditis elegans Embryo Peer-reviewed

    Xue Han, Jose-Eduardo Gomes, Cheryl L. Birmingham, Lionel Pintard, Asako Sugimoto, Paul E. Mains

    GENETICS　181　(3)　933-943　2009/03

    DOI： 10.1534/genetics.108.096016 　

    ISSN： 0016-6731

  35. [Germ granule formation and distribution in C. elegans].

    Hanazawa, M., Yonetani, M., Sugimoto, A.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　54　(16 Suppl)　2009

  36. Clearance of germ granules in the soma. Invited Peer-reviewed

    Sugimoto, Asako

    F1000 Biol Rep　1　49-49　2009

    DOI： 10.3410/B1-49 　

  37. The Caenorhabditis elegans DDX-23, a homolog of yeast splicing factor PRP28, is required for the sperm-oocyte switch and differentiation of various cell types Peer-reviewed

    Takafumi Konishi, Nobuko Uodome, Asako Sugimoto

    DEVELOPMENTAL DYNAMICS　237　(9)　2367-2377　2008/09

    DOI： 10.1002/dvdy.21649 　

    ISSN： 1058-8388

  38. A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex Peer-reviewed

    Reto Gassmann, Anthony Essex, Jia-Sheng Hu, Paul S. Maddox, Fumio Motegi, Asako Sugimoto, Sean M. O&apos;Rourke, Bruce Bowerman, Ian McLeod, John R. Yates, Karen Oegema, Iain M. Cheeseman, Arshad Desai

    GENES & DEVELOPMENT　22　(17)　2385-2399　2008/09

    DOI： 10.1101/gad.1687508 　

    ISSN： 0890-9369

  39. Efficient production of monoclonal antibodies recognizing specific structures in Caenorhabditis elegans embryos using an antigen subtraction method Peer-reviewed

    Kazumasa Takeda, Chie Watanabe, Hiroshi Qadota, Momoyo Hanazawa, Asako Sugimoto

    GENES TO CELLS　13　(7)　653-665　2008/07

    DOI： 10.1111/j.1365-2443.2008.01195.x 　

    ISSN： 1356-9597

    eISSN： 1365-2443

  40. EGG-3 regulates cell-surface and cortex rearrangements during egg activation in Caenorhabditis elegans Peer-reviewed

    Rika Maruyama, Nathalie V. Velarde, Richard Klancer, Scott Gordon, Pavan Kadandale, Jean M. Parry, Julie S. Hang, Jacob Rubin, Allison Stewart-Michaelis, Peter Schweinsberg, Barth D. Grant, Fabio Piano, Asako Sugimoto, Andrew Singson

    CURRENT BIOLOGY　17　(18)　1555-1560　2007/09

    DOI： 10.1016/j.cub.2007.08.011 　

    ISSN： 0960-9822

  41. Sequential functioning of the ECT-2 RhoGEF, RHO-1 and CDC-42 establishes cell polarity in Caenorhabditis elegans embryos Peer-reviewed

    Fumio Motegi, Asako Sugimoto

    NATURE CELL BIOLOGY　8　(9)　978-U80　2006/09

    DOI： 10.1038/ncb1459 　

    ISSN： 1465-7392

  42. Two phases of astral microtubule activity during cytokinesis in C-elegans embryos Peer-reviewed

    F Motegi, NV Velarde, F Piano, A Sugimoto

    DEVELOPMENTAL CELL　10　(4)　509-520　2006/04

    DOI： 10.1016/j.devcel.2006.03.001 　

    ISSN： 1534-5807

  43. Function of microtubules at the onset of cytokinesis

    Motegi, F., Sugimoto, A.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.　51　(11)　2006

  44. Cell polarization: lessons from C. elegans asymmetric cell division

    Motegi, F., Sugimoto, A.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.　51　(6 Suppl)　2006

  45. The C-elegans eyes absent ortholog EYA-1 is required for tissue differentiation and plays partially redundant roles with PAX-6 Peer-reviewed

    M Furuya, H Qadota, AD Chisholm, A Sugimoto

    DEVELOPMENTAL BIOLOGY　286　(2)　452-463　2005/10

    DOI： 10.1016/j.ydbio.2005.08.011 　

    ISSN： 0012-1606

  46. Caenorhabditis elegans geminin homologue participates in cell cycle regulation and germ line development. International-journal Peer-reviewed

    Ken-ichiro Yanagi, Takeshi Mizuno, Takashi Tsuyama, Shusuke Tada, Yumi Iida, Asako Sugimoto, Toshihiko Eki, Takemi Enomoto, Fumio Hanaoka

    The Journal of biological chemistry　280　(20)　19689-94　2005/05/20

    DOI： 10.1074/jbc.C500070200 　

    ISSN： 0021-9258

    More details Close

    Cdt1 is an essential component for the assembly of a pre-replicative complex. Cdt1 activity is inhibited by geminin, which also participates in neural development and embryonic differentiation in many eukaryotes. Although Cdt1 homologues have been identified in organisms ranging from yeast to human, geminin homologues had not been described for Caenorhabditis elegans and fungi. Here, we identify the C. elegans geminin, GMN-1. Biochemical analysis reveals that GMN-1 associates with C. elegans CDT-1, the Hox protein NOB-1, and the Six protein CEH-32. GMN-1 inhibits not only the interaction between mouse Cdt1 and Mcm6 but also licensing activity in Xenopus egg extracts. RNA interference-mediated reduction of GMN-1 is associated with enlarged germ nuclei with aberrant nucleolar morphology, severely impaired gametogenesis, and chromosome bridging in intestinal cells. We conclude that the Cdt1-geminin system is conserved throughout metazoans and that geminin has evolved in these taxa to regulate proliferation and differentiation by directly interacting with Cdt1 and homeobox proteins.

  47. High-throughput RNAi by soaking in Caenorhabditis elegans

    Sugimoto, A.

    RNA Interference Technology: From Basic Science to Drug Development　2005

    Publisher: RNA Interference Technology: From Basic Science to Drug Development

    DOI： 10.1017/CBO9780511546402.033 　

  48. Genome-wide analyses of gene expression and function in C. elegans

    Sugimoto, A., Kohara, Y.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　50　(16 Suppl)　2005

  49. Caenorhabditis elegans DAZ-1 is expressed in proliferating germ cells and directs proper nuclear organization and cytoplasmic core formation during oogenesis Peer-reviewed

    R Maruyama, S Endo, A Sugimoto, M Yamamoto

    DEVELOPMENTAL BIOLOGY　277　(1)　142-154　2005/01

    DOI： 10.1016/j.ydbio.2004.08.053 　

    ISSN： 0012-1606

  50. Type II platelet-activating fator-acetylhydrolase is essential for epithelial morphogenesis in Caenorhabditis elegans Peer-reviewed

    T Inoue, A Sugimoto, Y Suzuki, M Yamamoto, M Tsujimoto, K Inoue, J Aoki, H Arai

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　101　(36)　13233-13238　2004/09

    DOI： 10.1073/pnas.0405507101 　

    ISSN： 0027-8424

  51. Distinct developmental function of two Caenorhabditis elegans homologs of the cohesin subunit Scc1/Rad21 Peer-reviewed

    Y Mito, A Sugimoto, M Yamamoto

    MOLECULAR BIOLOGY OF THE CELL　14　(6)　2399-2409　2003/06

    DOI： 10.1091/mbc.E02-09-0603 　

    ISSN： 1059-1524

  52. Essential role of the C-elegans Arp2/3 complex in cell migration during ventral enclosure Peer-reviewed

    M Sawa, S Suetsugu, A Sugimoto, H Miki, M Yamamoto, T Takenawa

    JOURNAL OF CELL SCIENCE　116　(8)　1505-1518　2003/04

    DOI： 10.1242/jcs.00362 　

    ISSN： 0021-9533

  53. Protein phosphatase 4 is required for centrosome maturation in mitosis and sperm meiosis in C-elegans Peer-reviewed

    E Sumiyoshi, A Sugimoto, M Yamamoto

    JOURNAL OF CELL SCIENCE　115　(7)　1403-1410　2002/04

    ISSN： 0021-9533

    eISSN： 1477-9137

  54. The stem-loop binding protein CDL-1 is required for chromosome condensation, progression of cell death and morphogenesis in Caenorhabditis elegans Peer-reviewed

    Y Kodama, JH Rothman, A Sugimoto, M Yamamoto

    DEVELOPMENT　129　(1)　187-196　2002/01

    ISSN： 0950-1991

  55. Important role of junctophilin in nematode motor function Peer-reviewed

    M Yoshida, A Sugimoto, Y Ohshima, H Takeshima

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　289　(1)　234-239　2001/11

    DOI： 10.1006/bbrc.2001.5951 　

    ISSN： 0006-291X

  56. Many genomic regions are required for normal embryonic programmed cell death in Caenorhabditis elegans Peer-reviewed

    A Sugimoto, A Kusano, RR Hozak, WB Derry, JW Zhu, JH Rothman

    GENETICS　158　(1)　237-252　2001/05

    ISSN： 0016-6731

  57. Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi Peer-reviewed

    Maeda, I, Y Kohara, M Yamamoto, A Sugimoto

    CURRENT BIOLOGY　11　(3)　171-176　2001/02

    DOI： 10.1016/S0960-9822(01)00052-5 　

    ISSN： 0960-9822

    eISSN： 1879-0445

  58. Systematic functional analysis of the C. elegans genome

    Maeda, I., Sugimoto, A.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　46　(16 Suppl)　2001

  59. Caenorhabditis elegans homologue of the human azoospermia factor DAZ is required for oogenesis but not for spermatogenesis Peer-reviewed

    T Karashima, A Sugimoto, M Yamamoto

    DEVELOPMENT　127　(5)　1069-1079　2000/03

    ISSN： 0950-1991

    eISSN： 1477-9129

  60. kel-1, a novel Kelch-related gene in Caenorhabditis elegans, is expressed in pharyngeal gland cells and is required for the feeding process Peer-reviewed

    M Ohmachi, A Sugimoto, Y Iino, M Yamamoto

    GENES TO CELLS　4　(6)　325-337　1999/06

    DOI： 10.1046/j.1365-2443.1999.00264.x 　

    ISSN： 1356-9597

    eISSN： 1365-2443

  61. end-1 encodes an apparent GATA factor that specifies the endoderm precursor in Caenorhabditis elegans embryos Peer-reviewed

    JW Zhu, RJ Hill, PJ Heid, M Fukuyama, A Sugimoto, Priess, JR, JH Rothman

    GENES & DEVELOPMENT　11　(21)　2883-2896　1997/11

    ISSN： 0890-9369

  62. Type II myosin heavy chain encoded by the myo2 gene composes the contractile ring during cytokinesis in Schizosaccharomyces pombe Peer-reviewed

    C Kitayama, A Sugimoto, M Yamamoto

    JOURNAL OF CELL BIOLOGY　137　(6)　1309-1319　1997/06

    DOI： 10.1083/jcb.137.6.1309 　

    ISSN： 0021-9525

    eISSN： 1540-8140

  63. SCHIZOSACCHAROMYCES-POMBE ZFS1(+) ENCODING A ZINC-FINGER PROTEIN FUNCTIONS IN THE MATING PHEROMONE RECOGNITION PATHWAY Peer-reviewed

    J KANOH, A SUGIMOTO, M YAMAMOTO

    MOLECULAR BIOLOGY OF THE CELL　6　(9)　1185-1195　1995/09

    ISSN： 1059-1524

  64. DAD-1, AN ENDOGENOUS PROGRAMMED CELL-DEATH SUPPRESSOR IN CAENORHABDITIS-ELEGANS AND VERTEBRATES Peer-reviewed

    A SUGIMOTO, RR HOZAK, T NAKASHIMA, T NISHIMOTO, JH ROTHMAN

    EMBO JOURNAL　14　(18)　4434-4441　1995/09

    ISSN： 0261-4189

  65. SCHIZOSACCHAROMYCES-POMBE PAC2(+) CONTROLS THE ONSET OF SEXUAL DEVELOPMENT VIA A PATHWAY INDEPENDENT OF THE CAMP CASCADE Peer-reviewed

    H KUNITOMO, A SUGIMOTO, CRM WILKINSON, M YAMAMOTO

    CURRENT GENETICS　28　(1)　32-38　1995/06

    DOI： 10.1007/BF00311879 　

    ISSN： 0172-8083

    eISSN： 1432-0983

  66. BACULOVIRUS P35 PREVENTS DEVELOPMENTALLY PROGRAMMED CELL-DEATH AND RESCUES A CED-9 MUTANT IN THE NEMATODE CAENORHABDITIS-ELEGANS Peer-reviewed

    A SUGIMOTO, PD FRIESEN, JH ROTHMAN

    EMBO JOURNAL　13　(9)　2023-2028　1994/05

    ISSN： 0261-4189

    eISSN： 1460-2075

  67. SCHIZOSACCHAROMYCES-POMBE STE11+ ENCODES A TRANSCRIPTION FACTOR WITH AN HMG MOTIF THAT IS A CRITICAL REGULATOR OF SEXUAL DEVELOPMENT Peer-reviewed

    A SUGIMOTO, Y IINO, T MAEDA, Y WATANABE, M YAMAMOTO

    GENES & DEVELOPMENT　5　(11)　1990-1999　1991/11

    DOI： 10.1101/gad.5.11.1990 　

    ISSN： 0890-9369

  68. S-POMBE PAC1+, WHOSE OVEREXPRESSION INHIBITS SEXUAL DEVELOPMENT, ENCODES A RIBONUCLEASE-III-LIKE RNASE Peer-reviewed

    Y IINO, A SUGIMOTO, M YAMAMOTO

    EMBO JOURNAL　10　(1)　221-226　1991/01

    ISSN： 0261-4189

  69. FUNCTION OF THE 30-KD PROTEIN OF TOBACCO MOSAIC-VIRUS - INVOLVEMENT IN CELL-TO-CELL MOVEMENT AND DISPENSABILITY FOR REPLICATION Peer-reviewed

    T MESHI, Y WATANABE, T SAITO, A SUGIMOTO, T MAEDA, Y OKADA

    EMBO JOURNAL　6　(9)　2557-2563　1987/09

    ISSN： 0261-4189

Show all ︎Show first 5

Misc. 59

  1. in vivo 液-液相分離研究のパイオニア：生殖顆粒 Invited

    杉本 亜砂子

    実験医学　37　(9)　1393-1397　2019/05

  2. 体細胞分裂と減数分裂における紡錘体形成

    住吉 英輔, 杉本 亜砂子

    細胞工学　32　(3)　269-274　2013/03

    Publisher: 学研メディカル秀潤社

  3. 細胞分裂131年目の真実ー分子から動態へ：基礎の基礎

    杉本 亜砂子

    細胞工学　32　(3)　258-262　2013/03

    Publisher: 学研メディカル秀潤社

    ISSN： 0287-3796

  4. Cell polarity: centrosomes release signals for polarization.

    Sumiyoshi Eisuke, Sugimoto Asako

    Curr Biol　22　(8)　R281-R283　2012/04/24

    DOI： 10.1016/j.cub.2012.03.009 　

  5. Toward the second stage of recovery from the 3.11 Tohoku Earthquake

    Asako Sugimoto

    Genes to Cells　16　(7)　745-747　2011/07

    DOI： 10.1111/j.1365-2443.2011.01531.x 　

    ISSN： 1356-9597 1365-2443

  6. Imaging of Mitotic Spindle Dynamics in Caenorhabditis elegans Embryos

    Mika Toya, Yumi Iida, Asako Sugimoto

    MICROTUBULES: IN VIVO　97　359-372　2010

    DOI： 10.1016/S0091-679X(10)97019-2 　

    ISSN： 0091-679X

  7. 線虫における生殖顆粒の形成と分配—Germ granule formation and distribution in C. elegans—mRNAプログラム--多様性と非対称性の獲得戦略 ; RNAの局在化と翻訳制御

    花澤 桃世, 米谷 匡史, 杉本 亜砂子

    蛋白質核酸酵素 / 共立出版株式会社 [編]　54　(16)　2147-2152,2016　2009/12

    Publisher: 東京 : 共立出版

    ISSN： 0039-9450

  8. 線虫における生殖顆粒の形成と配分

    花澤桃世, 米谷匡史, 杉本亜砂子

    蛋白質 核酸 酵素 54　2147-2152　2009

  9. Clearance of germ granules in the soma.

    Sugimoto Asako

    F1000 Biol Rep　1　49-49　2009

    DOI： 10.3410/B1-49 　

  10. 培養細胞系を用いたC.elegans生殖顆粒構成因子PGL-3のドメイン解析

    米谷匡史, 花澤桃世, 杉本亜砂子, 杉本亜砂子

    生化学　2008

    ISSN： 0037-1017

  11. C.elegans生殖顆粒構成因子PGL-3のドメイン解析

    米谷匡史, 花澤桃世, 杉本亜砂子, 杉本亜砂子

    生化学　2007

    ISSN： 0037-1017

  12. 培養細胞系を用いた再構成で明らかになった線虫生殖顆粒の階層的形成メカニズム

    花澤桃世, 米谷匡史, 杉本亜砂子, 杉本亜砂子

    生化学　2007

    ISSN： 0037-1017

  13. in vivoイメージングのためのプローブ

    小西隆文, 杉本亜砂子

    実験がうまくいく蛍光・発光試薬の選び方と使い方　114-118　2007

    Publisher: 羊土社

  14. 体系的表現型解析で探る線虫の発生機構

    杉本亜砂子

    実験医学 25　151-157　2007

  15. ゲノムに書かれた発生プログラムを読み解くーー線虫をモデル系として

    杉本亜砂子

    ゲノムは何をどのように決めているのか？ 生命システムの理解へ向けて　101-112　2007

    Publisher: クバプロ

  16. 線虫の比較ゲノム

    杉本亜砂子

    比較ゲノム学から読み解く生命システム　105-110　2007

    Publisher: 秀潤社

  17. 対称から非対称へ：線虫受精卵における細胞極性確立メカニズム

    茂木文夫, 杉本亜砂子

    細胞工学 26　294-295　2007

  18. 折り返し地点に立って思うこと 接尾語としての-Biology

    杉本亜砂子

    蛋白質核酸酵素 51　51　(12)　1696-1698　2006

    Publisher: 共立出版

    ISSN： 0039-9450

  19. 2006年ノーベル生理学・医学賞は""RNA干渉”"の発見に

    杉本亜砂子

    Bionics 4　10-11　2006

  20. ノーベル賞受賞者の業績と人柄：生理学・医学賞 RNA干渉：二本鎖RNAによる新しい遺伝子発現抑制機構の発見

    杉本亜砂子

    科学 77　77　(1)　13-15　2006

    Publisher: 岩波書店

    ISSN： 0022-7625

  21. 線虫初期胚の非対称分裂から学ぶ""極性化""

    茂木文夫, 杉本亜砂子

    蛋白質核酸酵素 51　51　(6)　776-781　2006

    Publisher: 共立出版

    ISSN： 0039-9450

  22. 細胞質分裂の開始における微小管のはたらき

    茂木文夫, 杉本亜砂子

    蛋白質核酸酵素 51　51　(11)　1590-1595　2006

    Publisher: 共立出版

    ISSN： 0039-9450

  23. 線虫ゲノムの体系的発現・機能解析

    杉本亜砂子, 小原雄治

    蛋白質核酸酵素 50　50　(16)　2140-2145　2005

    Publisher: 共立出版

    ISSN： 0039-9450

  24. 線虫（C. elegans）におけるRNAiの応用

    飯田直子, 杉本亜砂子

    RNA工学の最前線　41-51　2005

    Publisher: シーエムシー出版

  25. Reverse genetics: RNAi by soaking.

    Sugimoto, A

    WormMethod　2005

  26. High-throughput RNAi in Caenorhabditis elegans: genome-wide screens and functional genomics

    A Sugimoto

    DIFFERENTIATION　72　(2-3)　81-91　2004/03

    DOI： 10.1111/j.1432-0436.2004.07202004.x 　

    ISSN： 0301-4681

    eISSN： 1432-0436

  27. 動物のゲノム解析：線虫

    杉本亜砂子

    ゲノミクス・プロテオミクスの新展開　338-346　2004

    Publisher: エヌ・ティー・エス

  28. 生物種別RNAi実験プロトコール：線虫

    飯田直子, 魚留信子, 杉本亜砂子, 門田裕志

    改訂 RNAi実験プロトコール　45-55　2004

    Publisher: 羊土社

  29. 線虫C. elegans研究の創始者たち3人にノーベル賞

    杉本亜砂子

    実験医学 20　2643-2644　2003

  30. 生物種別RNAi実験プロトコール：線虫

    作本直子, 魚留信子, 杉本亜砂子, 門田裕志

    RNAi実験プロトコール　37-47　2003

    Publisher: 羊土社

  31. 2002年ノーベル医学生理学賞 線虫C. elegans研究とプログラム細胞死

    杉本亜砂子

    現代化学 382　(382)　20-23　2003

    Publisher: 東京化学同人

    ISSN： 0386-961X

  32. プログラム細胞死

    杉本亜砂子

    線虫 究極のモデル生物　45-55　2003

    Publisher: シュプリンガー・フェアラーク東京

  33. 線虫C. elegansのゲノム機能解析

    杉本亜砂子

    第27回阿蘇シンポジウム 2003「医学をゲノムする」　2003

    Publisher: 南山堂

  34. RNA干渉法

    杉本亜砂子, 門田裕志

    線虫ラボマニュアル　115-133　2003

    Publisher: シュプリンガー・フェアラーク東京

  35. RNAiによる線虫遺伝子機能の体系的解析

    前田郁麻, 杉本亜砂子

    分子細胞治療 1　121-124　2002

  36. ゲノム工学：RNA

    杉本亜砂子

    ゲノム医学 2　2　(2)　181-186　2002

    Publisher: メディカルレビュー社

    ISSN： 1346-4671

  37. RNAi---その分子機構と応用

    杉本亜砂子

    化学と生物 40　40　(11)　713-718　2002

    Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry

    DOI： 10.1271/kagakutoseibutsu1962.40.713 　

    ISSN： 0453-073X

  38. C.elegansにおけるミラー・ディーカー症原因遺伝子産物LIS1の解析

    鈴木 優香, 井上 貴雄, 古泉 博之, 丸橋 裕子, 青木 淳賢, 杉本 亜砂子, 山本 正幸, 井上 圭三, 新井 洋由

    生化学　73　(8)　1074-1074　2001/08

    Publisher: (公社)日本生化学会

    ISSN： 0037-1017

    eISSN： 2189-0544

  39. 線虫ゲノムの体系的機能解析

    前田郁麻, 杉本亜砂子

    蛋白質核酸酵素 46,　2432-2435　2001

  40. 他の生物におけるゲノム解析の現状と未来：線虫

    杉本亜砂子

    植物のゲノム研究プロトコール　237-239　2001

    Publisher: 秀潤社

  41. 線虫ゲノムの機能解析

    杉本亜砂子

    ゲノムから個体へ 生命システムの理解に向けて　16-29　2001

    Publisher: 中山書店

  42. 体系的RNA干渉法による線虫C. elegansの発生に必須な遺伝子群の探索

    杉本亜砂子

    医学のあゆみ 199　9945-9949　2001

  43. 線虫をモデル系とした細胞死研究の進展

    杉本亜砂子

    Molecular Medicine 37　37　(4)　446-452　2000

    Publisher: 中山書店

    ISSN： 0918-6557

  44. 線虫における生殖細胞の形成

    杉本亜砂子

    生殖細胞 その誕生と振る舞い　33-43　2000

    Publisher: クバプロ

  45. 線虫C. elegansにおける生殖細胞形成過程の逆遺伝学的解析

    辛島健, 杉本亜砂子

    細胞工学 19　19　(6)　913-921　2000

    Publisher: 秀潤社

    ISSN： 0287-3796

  46. kel-1, a novel Kelch-related gene in Caenorhabditis elegans is expressed in pharyngeal gland cells and is required for the feeding process. (vol 4, pg 325, 1999)

    M Ohmachi, A Sugimoto, Y Iino, M Yamamoto

    GENES TO CELLS　4　(8)　487-487　1999/08

    ISSN： 1356-9597

  47. 細胞内II型PAFアセチルハイドロラーゼノックアウトC.elegansの解析

    井上 貴雄, 青木 淳賢, 新井 洋由, 井上 圭三, 杉本 亜砂子, 辛島 健, 山本 正幸

    日本薬学会年会要旨集　119年会　(3)　45-45　1999/03

    Publisher: (公社)日本薬学会

    ISSN： 0918-9823

  48. 線虫

    杉本亜砂子

    新アポトーシス実験法　158-163　1999

    Publisher: 羊土社

  49. C. elegansについて知っている2、3の事柄

    杉本亜砂子

    実験室の小さな生きものたち　24-28　1999

    Publisher: 羊土社

  50. Mutation that affects the division axis in the first cleavage of C. elegans

    SUMIYOSI Eisuke, SUGIMOTO Asako, YAMAMOTO Masayuki

    21　638-638　1998/12/01

  51. A C. elegans homologue of human DAZ is essential in meiosis during oogenesis

    KARASHIMA Takeshi, SUGIMOTO Asako, YAMAMOTO Masayuki

    21　638-638　1998/12/01

  52. Genetic and molecular characterization of the C. elegans cdl-1 mutant

    KODAMA Yuki, SUGIMOTO Asako, ROTHMAN Joel H., YAMAMOTO Masayuki

    21　638-638　1998/12/01

  53. C. elegansについて知っている2、3の事柄

    杉本亜砂子

    実験医学 16　549　1998

  54. ネマトーダモデルとアポトーシス関連遺伝子

    杉本亜砂子

    アポトーシスと疾患「基礎編」　32-43　1998

    Publisher: 医薬ジャーナル社

  55. 線虫C. elegansのプログラム細胞死

    杉本亜砂子

    医学のあゆみ 187　447-451　1998

  56. モデル系としてのC. elegans −アポトーシス制御機構の種を越えた普遍性

    杉本亜砂子

    実験医学 15　65-69　1997

  57. 線虫におけるプログラム細胞死の分子機構

    杉本亜砂子

    アポトーシス研究の新展開　(35)　3-12　1997

    Publisher: 東京化学同人

    ISSN： 0910-4747

  58. アポトーシスの諸過程を解析する手段 (4) 遺伝学的手法 a) C. elegans.

    杉本亜砂子

    最新アポトーシス実験法　217-226　1995

    Publisher: 羊土社

  59. 減数分裂.「Annual Review 細胞生物学1991」

    杉本亜砂子, 山本正幸

    Annual Review 細胞生物学1991　181-191　1991

    Publisher: 中外医学社

Show all ︎Show first 5

Books and Other Publications 2

  1. 細胞分裂131年目の真実 : 分子から動態へ : 特集

    杉本, 亜砂子

    学研メディカル秀潤社,学研マーケティング (発売)　2013

    ISBN: 9784780901405

  2. 線虫C. elegansにおける生殖細胞形成の分子機構の解析

    杉本, 亜砂子

    杉本亜砂子　2002

Presentations 185

  1. Caenorhabditis auriculariaeにおけるプログラムされたDNA削減（PDE)のメカニズムを理解するためのバイオインフォマティクスアプローチ

    孫思墨, 上床直紀, 肖楽雲, 佐藤一輝, 春田奈美, 杉本亜砂子, 菊地泰生

    第47回日本分子生物学会年会　2024/11

  2. 近縁線虫のVasaオーソログGLHの比較解析からせまる、生殖類粒形成メカニズムの普遍性と多様性

    草野太智, 野呂美波, パンウィキュ, 生井聡史, 春田奈美, 杉本亜砂子

    第47回日本分子生物学会年会　2024/11

  3. 線虫Caenorhabditis elegansとその姉妹種Caenorhabditis inopinataの休眠誘導メカニズムの比較解析

    飯塚稜, 大村駿, 春田奈美, 杉本亜砂子

    第47回日本分子生物学会年会　2024/11

  4. Caenorhabditis auriculariaeにおけるプログラムされた染色体削減の分子メカニズムの解明をめざして

    春田奈美, 上床直紀, 孫思墨, 菊地泰生, 杉本亜砂子

    第47回日本分子生物学会年会　2024/11

  5. 線虫C. elegans におけるLacI/LacO システムを用いた人工動原体の構築 Construction of artificial kinetochores using the LacI/LacO system in C.eleg

    吉田洋輝, 春田奈美, 堀哲也, 深川竜郎, 杉本亜砂子

    日本遺伝学会 第９６回大会　2024/09

  6. Comparative analysis of early embryonic dynamics of P. pacificus and C. elegans

    Rikako Hata, Daichi Sasaki, Taichi Kusano, Satoshi Namai, Nami Haruta, Asako Sugimoto

    2024/08

  7. Contribution of telomere-binding proteins to programmed DNA elimination in Caenorhabditis auriculariae / 線虫Caenorhabditis auriculariae の染色体削減におけるテ ロメア結合タンパク質群の寄与

    上床直紀, 孫思墨, 中村時生, 春田奈美, 菊地泰生, 杉本亜砂子

    線虫研究の未来を創る会 2024　2024/08

  8. The dauer state of C. inopinata can be induced by fig-derived bacteria

    Ryo Iitsuka, Shun Oomura, Nami Haruta, Asako Sugimoto

    2024/08

  9. 栄養物質イメージングに向けた極限環境に棲む胎生線虫Tokorhabditis tufae の形質転換手法の確立

    山下達矢, 蓮見麻有, 春田奈美, 杉本亜砂子, 新屋良治

    線虫研究の未来を創る会 2024　2024/08

  10. 線虫P. pacificus 生殖顆粒における、GLH の機能保存性

    草野太智, 野呂美波, パンウィキュ, 生井聡史, 春田奈美, 杉本亜砂子

    線虫研究の未来を創る会 2024　2024/08

  11. 線⾍Caenorhabditis elegansの⽣殖腺維持におけるヒストンバリアントH2A.Zの機能解析

    堀井 菜摘, 北川 紗帆, 春田 奈美, 鐘巻 将人, 杉本 亜砂子, 原田 昌彦

    第46回日本分子生物学会年会　2023/12

  12. Caenorhabditis auriculariae における染⾊体削減の解析

    上床直紀, 孫思墨, 中村時生, 春田奈美, 菊地泰生, 杉本亜砂子

    第46回日本分子生物学会年会　2023/12

  13. 線⾍C. elegansにおけるLacI/LacOを⽤いた⼈為的動原体形成：ホロセントリック染⾊体からモノ セントリック染⾊体への⼈為的な変換をめざして

    吉田 洋輝, 春田 奈美, 堀 哲也, 深川 ⻯郎, 杉本 亜砂子

    第46回日本分子生物学会年会　2023/12

  14. 線⾍種間⽐較から明らかにする⽣殖顆粒の普遍性と多様性

    草野 太智, 野呂 美波, パン イギュ, 生井 聡史, 春田 奈美, 杉本 亜砂子

    第46回日本分子生物学会年会　2023/12

  15. 線⾍の⽣殖システム転換に関与した⾮コード領域のゲノム⽐較解析

    玉川 克典, Mehmet Dayi, 孫 思墨, 菊地 泰生, 春田 奈美, 杉本 亜砂子, 牧野 能士

    第46回日本分子生物学会年会　2023/12

  16. 線虫近縁種をモデル系とした種に固有の体サイズを実現する遺伝的制御機構の解明

    大村 駿, 春田 奈美, 玉川 克典, 牧野 能士, 杉本 亜砂子

    日本遺伝学会第95回大会　2023/09

  17. What can we learn from the comparative analysis of the model nematode Caenorhabditis elegans and its sister species Caenorhabditis inopinata ? モデル線虫 Caenorhabditis elegans とその姉妹種 Caenorhabditis inopinata と の比較解析から何がわかるか Invited

    2023/08/31

  18. The effect of transposable elements on gene expression levels in Caenorhabditis inopinata, whose characteristics differ greatly from those of the sibling species Caenorhabditis elegans.

    2023/09

  19. Evolutionary changes of non‐coding elements associated with transition of sexual mode in the Caenorhabditis nematode

    2023/09

  20. Caenorhabditis auriculariae における染色体削減の分子機構の解析

    上床直紀, 春田奈美, 中村時生, 孫思墨, 菊地泰生, 杉本亜砂子

    線虫研究の未来を創る会 2023　2023/08

  21. Comparative analysis of germ granules between Caenorhabditis elegans and Pristionchus pacificus

    Taichi kusano, Minami Noro, Uikyu Bang, Satoshi Namai, Nami Haruta, Asako Sugimoto

    線虫研究の未来を創る会 2023　2023/08

  22. C. elegans ゲノムの人為的再編成

    高島幹太, 春田奈美, 米秀之, 河野宏光, 太田邦史, 杉本亜砂子

    線虫研究の未来を創る会 2023　2023/08

  23. dauer 出現頻度の低いC. inopinata のdauer シグナル経路解析

    Ryo Iitsuka, Shun Oomura, Ryuhei Hatanaka, Katsunori Tamagawa, Nami Haruta, Asako Sugimoto

    線虫研究の未来を創る会 2023　2023/08

  24. 雌雄同体の進化に関与した非コード領域のゲノム比較解析

    玉川克典, Mehmet Dayi, 孫思墨, 菊地泰生, 春田奈美, 杉本亜砂子, 牧野能士

    線虫研究の未来を創る会 2023　2023/08

  25. 線虫C. elegansにおける中心小体タンパク質SAS-7のドメイン解析

    中條桃江, 春田奈美, 杉本亜砂子

    第2回 細胞分裂研究会　2023/07

  26. Diversity and evolution of germ granules in nematodes Invited

    Asako Sugimoto, Taichi Kusano, Minami Noro, Uikyu Bang, Satoshi Namai, Nami Haruta

    2023/07/23

  27. The tissue-specific role of the unconventional components of the g-tubulin complex in C. elegans.Comments

    2023/07

  28. Construction of a dauer specific marker for the analysis of the dauer signaling pathway in C. Inopinata, which has a low dauer frequency of dauer formation

    Ryo Iitsuka, Shun Oomura, Ryuhei Hatanaka, Katsunori Tamagawa, Nami Haruta, Asako Sugimoto

    2023/06

  29. The germline-specific role of the unconventional components of the γ-tubulin complex in C. elegans

    Nami Hartuta, Asako Sugimoto

    Cold Spring Harbor Asia Conference on Cilia & Centrosomes　2023

  30. Species- and tissue-specific microtubule organization in nematodes Invited

    Asako Sugimoto

    Cold Spring Harbor Asia Conference on Cilia & Centrosomes　2023/03/01

  31. 線⾍近縁種間における性決定に違いを引き起こしたゲノム変化

    畑中龍平, 春田, 奈美, 菊地, 泰生, 杉本 亜砂子

    第45回 日本分子生物学会年会　2022/12/02

  32. 線⾍受精卵における細胞ダイナミクスの進化と多様性

    杉本 亜砂子

    第45回 日本分子生物学会年会　2022/11/30

  33. 線虫近縁種間において初期胚細胞動態の差異をもたらす遺伝的要因の解析

    大村駿, 京田耕司, 大浪修一, 春田奈美, 杉本亜砂子

    日本遺伝学会第94回年会　2022/09

  34. Centrosome maturation requires phosphorylation-mediated sequential domain interactions of SPD-5 Invited

    Asako Sugimoto

    Cilia & Centrosomes Pre-meeting Webinar (Virtual)　2022/09/14

  35. 線虫C. elegans における感覚繊毛神経特異的なチューブリンアイソタイプの特性解析

    西田 桂, 土屋 賢汰, 春田 奈美, 杉本 亜砂子

    線虫研究の未来を創る会2022　2022/08

  36. Comparative analysis of germ granules between Caenorhabditis elegans and Pristionchus pacificus

    Taichi kusano, Minami Noro, Uikyu Bang, Satoshi Namai, Nami Haruta, Asako Sugimoto

    2022/08

  37. Comparative analysis of cellular dynamics of C. elegans and C. inopinata zygotes

    Shun Oomura, Shuichi Onami, Koji Kyoda, Nami Haruta, Asako Sugimoto

    2022/08

  38. C. elegans とその近縁種C. inopinata のdauer 移行識別マーカー株の作出

    Ryo Iitsuka, Shun Oomura, Nami Haruta, Asako Sugimoto

    線虫研究の未来を創る会 2022　2022/08

  39. Comparative analysis of cellular dynamics of C. inopinata and C. elegans zygotes

    Shun Oomura, Shuichi Onami, Koji Kyoda, Nami Haruta, Asako Sugimoto

    Evolutionary Biology of Caenorhabditis and Other Nematodes　2022/06

  40. Significant differences in the sex determination pathway between C. elegans and C. inopinata

    Ryuhei Hatanaka, Nami Haruta, Taisei Kikuchi, Asako Sugimoto

    Evolutionary Biology of Caenorhabditis and Other Nematodes　2022/06

  41. Unusual chromosome dynamics and maintenance in a parasitic nematode, Strongyloides ratti

    Asuka Kounosu, Simo Sun, Kazunori Murase, Vicky Hunt, Akemi Yoshida, Haruhiko Maruyama, Asako Sugimoto, Taisei Kikuchi

    2022/05/28

  42. 近縁な線虫種間の体サイズ進化における転移因子の役割

    河原 数馬, 稲田, 垂穂, 田中, 龍聖 Mehmet DAYI, 菊地, 泰生, 杉本, 亜砂子, 河田 雅圭

    第69回日本生態学会大会　2022/03

  43. 線虫近縁種比較から解き明かす生殖システム進化 Invited

    杉本亜砂子

    第44回日本分子生物学会年会　2021/12/02

  44. 線虫Pristionchus pacificusにおける生殖顆粒構成因子の同定と機能解析

    野呂美波, パン・ウィキュ, 生井聡史, 春田奈美, 杉本亜砂子

    第44回日本分子生物学会年会　2021/12

  45. 線虫C. elegansを用いた感覚神経特異的チューブリンアイソタイプ群の特性解析

    西田 桂, 土屋 賢汰, 春田 奈美, 池田 真教, 田中 耕三, 杉本 亜砂子

    第44回日本分子生物学会年会　2021/12

  46. Analysis of germ granule components in the nematode Pristionchus pacificus International-presentation

    Minami Noro, Bang Uikyu, Satoshi Namai, Nami Haruta, Asako Sugimoto

    2nd International Pristionchus Meeting　2021/10/12

  47. Distinct regulation of actin and microtubule cytoskeletons during the first mitotic divisions in Pristionchus pacificus and Caenorhabditis elegans

    Asako Sugimoto

    2nd International Pristionchus Meeting　2021/10

  48. Comparative analysis of germ granules between Caenorhabditis elegans and Pristionchus pacificus

    Minami Noro, Uikyu Bang, Satoshi Namai, Nami Haruta, Asako Sugimoto

    2021/08/31

  49. Comparative analysis of cellular dynamics of C. elegans and C. inopinata zygotes

    Shun Oomura, Shuichi Onami, Koji Kyoda, Nami Haruta, Asako Sugimoto

    2021/08/31

  50. 近縁種と大きく異なる体サイズを獲得した線虫における転移因子の影響

    河原 数馬, 稲田 垂穂, 田中 龍聖, Mehmet Dayi, 菊地 泰生, 杉本 亜砂子, 河田 雅圭

    第23回進化学会　2021/08

  51. Chromosome dynamics in sex determination of the parthenogenetic nematode Strongyloides ratti

    Asuka Kounosu, Simo Sun, Kazunori Murase, Vicky Hunt, Akemi Yoshida, Haruhiko Maruyama, Asako Sugimoto, Taisei Kikuchi

    23rd international C. elegans conference　2021/06

  52. Reproductive incompatibility among populations of Caenorhabditis inopinata

    Ryusei Tanaka, Akemi Yoshida, Asako Sugimoto, Taisei Kikuchi

    23rd international C. elegans conference　2021/06

  53. An alternative ERGO-1 pathway in a sibling species of C. elegans, C. inopinata

    Taisei Kikuchi, Vicky Hunt, Simo Sun, Ryusei Tanaka, Akemi Yoshida, Asako Sugimoto

    23rd international C. elegans conference　2021/06

  54. Distinct properties of broadly-expressed and tissue-specific tubulin isotypes examined by ectopic and heterologous expression

    Kei Nishida, Kenta Tsuchiya, Yu Honda, Hiroyuki Obinata, Shizuka Onodera, Nami Haruta, Masanori Ikeda, Kozo Tanaka, Asako Sugimoto

    23rd international C. elegans conference　2021/06

  55. Comparative analysis of cellular dynamics of C. inopinata and C. elegans zygotes

    Shun Oomura, Shuichi Onami, Koji Kyoda, Nami Haruta, Asako Sugimoto

    23rd international C. elegans conference　2021/06

  56. Significant differences in the sex determination pathways between C. inopinata and C. elegans

    Ryuhei Hatanaka, Yuki Hoshi, Nami Haruta, Taisei Kikuchi, Asako Sugimoto

    23rd international C. elegans conference　2021/06

  57. Chromosome analysis on sex determination system in the parthenogenetic nematodes Strongyloides spp.

    Asuka Kounosu, Simo Sun, Akemi Yoshida, Kazunori Murase, Vicky L. Hunt, Haruhiko Maruyama, Asako Sugimoto, Taisei Kikuchi

    2020/12/04

  58. 線虫近縁種間における性決定の違いを引き起こすゲノム変化

    畑中 龍平, 星 優希, 春田 奈美, 菊地 泰生, 杉本 亜砂子

    第43回日本分子生物学会年会　2020/12

  59. Caenorhabditis elegans とPristionchus pacificusにおける生殖類粒の比較解析

    野呂 美波, パン ウィギュ, 生井 聡史, 春田 奈美, 杉本 亜砂子

    第43回日本分子生物学会年会　2020/12

  60. 線虫C.elegansにおける中心体周辺物質の足場形成機構の解析

    中條 桃江, 狩野 ひかる, 春田 奈美, 杉本 亜砂子

    第43回日本分子生物学会年会　2020/12

  61. 中心体と組織特異的な微小管形成中心

    春田 奈美, 中條 桃江, 杉本 亜砂子

    第43回日本分子生物学会年会　2020/12

  62. 近縁種から大きな進化的変化を生じたCaenorhabditis inopinataにおいて、転位因子が近傍遺伝子の発現に与える影響

    河原数馬, 稲田垂穂, 田中龍聖, Mehmet Dayi, 菊地泰生, 杉本亜砂子, 河田雅圭

    日本進化学会第22回大会　2020/09/07

  63. Early embryogenesis of Caenorhabditis inopinata, the closest species of Caenorhabditis elegans

    Shun Oomura, Yuki Matsumura, Nami Haruta, Asako Sugimoto

    Ecology, Evolution and Genomics of C. elegans and Other Nematodes　2020/06

  64. Comprehensive expression analysis of tubulin isotypes using GFP-knock-in strains in C.elegans

    Kei Nishida, Kenta Tsuchiya, Yu Honda, Hiroyuki Obinata, Shizuka Onodera, Nami Haruta, Masanori Ikeda, Kozo Tanaka, Asako Sugimoto

    EMBO/EMBL Symposia Microtubules　2020/06

  65. Unconventional components of the gamma-tubulin complex in C. elegans and their evolutionary implications Invited

    Asako Sugimoto

    EMBO/EMBL Symposia Microtubules　2020/06

  66. Scaffold Assembly of Pericentriolar Material in Caenorhabditis elegans.

    M. Nakajo, H. Kano, N. Haruta, A. Sugimoto

    ASCB | EMBO 2019 meeting　2019/12/07

  67. The Roles of Two γ-tubulin-associated Proteins in Embryos and Differentiated Cells in Caenorhabditis elegans.

    N. Haruta, N. Nakajo, C. Uchiyama, A. Sugimoto

    ASCB | EMBO 2019 meeting　2019/12/07

  68. 線虫におけるゲノムスケール変化と表現型進化 Invited

    杉本 亜砂子, 大村 駿, 星 優希, 畑中 龍平, 春田 奈美, 稲田 垂穂, 牧野 能士, 河田 雅圭, 菊地 泰生

    第42回日本分子生物学会年会　2019/12/03

  69. 線虫C.elegansにおける神経特異的チューブリンアイソタイプの機能解析

    西田 桂, 土屋 賢汰, 本多 優, 小日向 寛之, 小野寺 静, 春田 奈美, 池田 真教, 田中 耕三, 杉本 亜砂子

    第42回日本分子生物学会年会　2019/12/03

  70. 線虫から見た微小管の多様性と進化 Invited

    杉本 亜砂子

    第92回日本生化学会大会　2019/09/18

  71. C.elegansとC.inopinataを用いた個体サイズ制御機構の比較解析

    大村 駿, 杉本 亜砂子

    日本遺伝学会第91回大会　2019/09/11

  72. Elucidation of genomic changes that cause difference of sex determination among nematode species

    2019/08/21

  73. Analysis of tubulin isotype expression patterns during the development of C. elegans

    2019/08/21

  74. 線虫C. elegans における神経特異的チューブリンアイソタイプの機能解析

    西田 桂, 杉本 亜砂子

    線虫研究の未来を創る会2019　2019/08/21

  75. 線虫C. elegans における中心小体周辺物質の足場形成機構の解析

    中條 桃江, 杉本 亜砂子

    線虫研究の未来を創る会2019　2019/08/21

  76. Early embryogenesis of C. inopinata, a sibling species of C. elegans

    2019/08/21

  77. Why is C. inopinata larger than C. elegans?

    2019/08/21

  78. The role of C. elegans γ-TuRC specific components in embryo and differentiated cells

    2019/08/21

  79. 線虫C. elegans γ -tubulin 複合体構成因子の組織特異的な役割

    春田 奈美, 内山 智尋, 杉本 亜砂子

    第71回日本細胞生物学会 合同年次大会　2019/06/24

  80. "線虫C. elegansにおけるチューブリンアイソタイプの発現パターンおよび機能の 網羅的解析"

    西田 桂, 土屋 賢汰, 本多 優, 小日向 寛之, 小野寺 静, 春田 奈美, 池田 真教, 田中 耕三, 杉本 亜砂子

    第71回日本細胞生物学会 合同年次大会　2019/06/24

  81. slc-25a46 is required for proper localization of mitochondria and mitochondrial fusion

    H. Obinata, A. Sugimoto, S. Niwa

    22nd International C.elegans Conference　2019/06/20

  82. Early embryogenesis of C. inopinata, a sibling species of C.elegans

    S. Oomura, Y. Hoshi, Y. Matsumura, N. Haruta, A. Sugimoto

    22nd International C.elegans Conference　2019/06/20

  83. 細胞生物学・発生生物学と生態学の接点〜線虫種間比較研究をモデルケースとして Invited

    杉本 亜砂子

    第66回日本生態学会大会　2019/03/15

  84. C.elegansとその近縁種P. pacificusを用いた生殖顆粒の進化細胞生物学的解析

    Uikyu Bang, 生井聡史, 杉本亜砂子

    第41回日本分子生物学会年会　2018/11/28

  85. 線虫C.elegansにおけるチューブリンアイソタイプの発現パターンおよび機能の網羅的解析

    西田桂, 土屋賢汰, 小日向寛之, 小野寺静, 春田奈美, 杉本亜砂子

    第41回日本分子生物学会年会　2018/11/28

  86. 中心体形成に必須なC.elegans SPD-5のin vivoドメイン解析

    中條桃江, 狩野ひかる, 春田奈美, 杉本亜砂子

    第41回日本分子生物学会年会　2018/11/28

  87. 線虫slc-25A46は、神経内でミトコンドリアの正常な分布と融合に必要である

    小日向寛之, 杉本亜砂子, 丹羽伸介

    第41回日本分子生物学会年会　2018/11/28

  88. 線虫C.elegansの卵形成におけるγ(gamma)-チューブリン複合体構成因子の役割

    春田 奈美, 内山 智尋, 杉本 亜砂子

    日本遺伝学会 第90回大会　2018/09/18

  89. Caenorhabditis elegansの近縁種にお帰る遺伝子操作法の開発

    春田 奈美, 生井 聡史, 津山 研二, 杉本 亜砂子

    2018年度日本線虫学会定期大会（第26回大会）　2018/09/04

  90. An actin-dependent spindle positioning mechanism in the zygote of Pristionchus pacificus

    Satoshi Namai, Daichi Sasaki, Asako Sugimoto

    C.elegans development, cell biology and gene expression　2018/06/13

  91. Distinct regulatory mechanisms control the first asymmetric cell division of Pristionchus pacificus and Caenorhabditis elegans International-presentation

    SUGIMOTO Asako

    The 2nd Indian C. elegans Meeting　2018/02/23

  92. Comparative analysis of polarity establishment mechanisms in C. elegans and P. pacificus zygotes

    Daichi Sasaki, Satoshi Namai, Asako Sugimoto

    2017年度生命科学系学会合同年次大会（CnBio2017）　2017/12/06

  93. 線虫C. elegansとP. pacificus初期胚の細胞極性化機構の比較解析

    佐々木 大地, 生井 聡史, 杉本 亜砂子

    2017年度生命科学系学会合同年次大会（CnBio2017）　2017/12/06

  94. 線虫TIMPsはADAMTSプロテアーゼと相互作用することで生殖巣形態形成を制御する

    久保田 幸彦, 西脇 清二, 杉本 亜砂子

    2017年度生命科学系学会合同年次大会（CnBio2017）　2017/12/06

  95. 線虫C. elegansとその姉妹種C. inopinataにおける性決定遺伝子経路の比較解析

    星 優希, 津山 研二, 杉本 亜砂子

    2017年度生命科学系学会合同年次大会（CnBio2017）　2017/12/06

  96. 線虫γ-チューブリン複合体の会合と制御メカニズム

    春田 奈美, 本多 優, 住吉 英輔, 寺澤 匡博, 杉本 亜砂子

    2017年度生命科学系学会合同年次大会（CnBio2017）　2017/12/06

  97. 線虫C.elegansにおけるチューブリンアイソタイプの微小管機能多様性への寄与

    小日向 寛之, 土屋 賢汰, 本多 優, 杉本 亜砂子

    2017年度生命科学系学会合同年次大会（CnBio2017）　2017/12/06

  98. 線虫C. elegansのTIMPはADAMTSプロテアーゼと遺伝学的に相互作用することで生殖巣の発生を制御する

    久保田幸彦, 西脇清二, 杉本亜砂子

    日本動物学会 第88回富山大会　2017/09/21

  99. C.elegansと姉妹種を用いた個体サイズ制御メカニズムの解析

    津山研二, 杉本亜砂子

    日本遺伝学会第89回大会　2017/09/13

  100. 線虫γ-チューブリン複合体の普遍性と特殊性

    春田 奈美, 本多 優, 住吉 英輔, 寺澤 匡博, 杉本 亜砂子

    日本遺伝学会第89回大会　2017/09/13

  101. C. elegansとその姉妹種の性決定遺伝子経路の比較解析

    星 優希, 津山研二, 杉本亜砂子

    日本動物学会・平成29年度東北支部大会　2017/07/29

  102. 線虫TIMPsはADAMTSと相互作用することで生殖巣形態形成を制御する

    久保田幸彦, 西脇清二, 杉本亜砂子

    日本動物学会・平成29年度東北支部大会　2017/07/29

  103. 線虫C. elegansとP. pacificus初期胚の細胞極性化機構の比較解析

    佐々木大地, 生井聡史, 杉本亜砂子

    日本動物学会・平成29年度東北支部大会　2017/07/29

  104. Evolutionary insights into the C. elegans biology from the morphology, ecology and genome of the sister species Caenorhabditis sp. 34 International-presentation

    T. Kikuchi, I. Tsai, R. Tanaka, G. Woodruff, J. Wang, M. Berriman, P. Sternberg, A. Sugimoto, N. Kanzak

    21st International C. elegans Conference　2017/06/21

  105. Contribution of tubulin isotypes to diverse microtubule dynamics and functions in vivo International-presentation

    Kenta Tsuchiya, Yu Honda, Hiroyuki Obinata, Asako Sugimoto

    21st International C. elegans Conference　2017/06/21

  106. Tissue inhibitors of metalloproteinases (TIMPs) genetically interact with ADAMTS proteases and regulate gonadal development in C. elegans International-presentation

    Y. Kubota, K. Nishiwaki, A. Sugimoto

    21st International C. elegans Conference　2017/06/21

  107. Establishing genetic techniques to study Caenorhabditis sp. 34, a sister species of C. elegans International-presentation

    Asako Sugimoto, Kenji Tsuyama, Yuki Hoshi, Satoshi Namai, Ryohei Kumagai, Natsumi Kanzaki, Taisei Kikuchi

    21st International C. elegans Conference　2017/06/21

  108. Contribution of tubulin isotypes to diverse microtubule dynamics in vivo

    Kenta Tsuchiya, Yu Honda, Hiroyuki Obinata, Asako Sugimoto

    第69回 日本細胞生物学会大会　2017/06/13

  109. 線虫C. elegans SAS-7は中心小体複製と間期PCMの形成に関与する

    春田奈美, 吉井千尋, 内谷進介, 住吉英輔, 寺澤匡博, 杉本亜砂子

    第69回 日本細胞生物学会大会　2017/06/13

  110. 線虫C. elegansにおけるチューブリンアイソタイプの発現パターンおよび機能の網羅的解析

    小日向寛之, 土屋賢汰, 本多優, 杉本亜砂子

    第69回 日本細胞生物学会大会　2017/06/13

  111. Contribution of tubulin isotypes to diverse microtubule dynamics in vivo International-presentation

    Kenta Tsuchiya, Yu Honda, Hiroyuki Obinata, Asako Sugimoto

    International Symposium on Neural Precursor Cell Fate Determination, Differentiation and Neuronal Circuit Formation　2017/04/22

  112. C. elegans SAS-7 is required for the recruitment of γ-tubulin to the outer surface of centrioles at the late step of centriole assembly International-presentation

    Nami Haruta, Chihiro Yoshii, Shinsuke Uchiya, Eisuke Sumiyoshi, Masahiro Terasawa, Asako Sugimoto

    Cold Sring Harbor Asia Meeting "Cilia & Centrosomes"　2017/04/14

  113. 線虫は進化細胞生物学のモデル系となりうるか？

    第28回加藤記念研究助成贈呈式特別講演会　2017/03/03

  114. C. elegans SAS-7 is required for the recruitment of γ-tubulin to the outer surface of centrioles at the late step of centriole assembly International-presentation

    Nami Haruta, Chihiro Yoshii, Shinsuke Uchiya, Eisuke Sumiyoshi, Masahiro Terasawa, Asako Sugimoto

    SNU Bio-Symposium　2016/12/13

  115. 進化細胞生物学のモデル系としての線虫

    第39回日本分子生物学会年会　2016/11/30

  116. マトリックスメタロプロテアーゼ阻害因子（TIMP）様タンパク質は線虫生殖巣の形成に必須の役割を果たす

    久保田 幸彦, 西脇 清二, 杉本 亜砂子

    第39回日本分子生物学会年会　2016/11/30

  117. CRISPR-Cas9システムを用いた線虫C. elegans中心体タンパク質SPD-5のin vivoドメイン解析

    狩野 ひかる, 津山 研二, 春田 奈美, 杉本 亜砂子

    第39回日本分子生物学会年会　2016/11/30

  118. 線虫C. elegansの新規中心小体タンパク質GTAP-3/SAS-7の局在と機能

    春田 奈美, 吉井 千尋, 内谷 進介, 住吉 英輔, 寺澤 匡博, 杉本 亜砂子

    第39回日本分子生物学会年会　2016/11/30

  119. GTAP-3, a new γ-tubulin-associated protein is required for the efficient recruitment of γ-tubulin to centrosomes in C. elegans interphase cells International-presentation

    Nami Haruta, Shinsuke Uchiya, Nobuhito Oyama, Eisuke Sumuyoshi, Masahiro Terasawa, Yukihiko Kubota, Asako Sugimoto

    CDB Symposium "Cilia and Centrosomes"　2016/11/27

  120. Distinct contribution of different tubulin isotypes to microtubule dynamics

    Asako Sugimoto

    第54回日本生物物理学会年会　2016/11/25

  121. 線虫C. elegans GTAP-3は中心小体複製に寄与する

    春田奈美, 内谷進介, 吉井千尋, 住吉英輔, 寺澤匡博, 杉本亜砂子

    第89回日本生化学会大会　2016/09/25

  122. Caenorhabditis sp. 34 is a sister species to C. elegans with marked differences in morphology and ecology International-presentation

    Asako Sugimoto, Ryusei Tanaka, Kenji Tsunami, Satoshi Namai, Rhohei Kumagai, Takuya Shimura, Natsumi Kanzaki, Taisei Kikuchi

    C. elegans Development, Cell Biology and Gene Expression Meeting　2016/07/13

  123. Genetic and molecular tools for Caenorhabditis sp. 34, a bigger sister species of C. elegans International-presentation

    Kenji Tsunami, Satoshi Namai, Ryohei Kumagai, Takuya Shimura, Natsumi Kanzaki, Taisei Kikuchi, Asako Sugimoto

    C. elegans Development, Cell Biology and Gene Expression Meeting　2016/07/13

  124. Caenorhabditis sp. 34 is a sister species to C. elegans with marked differences in morphology and ecology International-presentation

    7th Asia-Pacific C. elegans Meeting　2016/06/25

  125. Comparative analysis of germ granules between Caenorhabditis elegans and its sister species Caenorhabditis sp. 34 International-presentation

    Ryohei Kumagai, Asako Sugimoto

    7th Asia-Pacific C. elegans Meeting　2016/06/25

  126. A TIMP-like gene is involved in gonadal development in C. elegans International-presentation

    Yukihiko Kubota, Kiyoji Nishiwaki, Asako Sugimoto

    7th Asia-Pacific C. elegans Meeting　2016/06/25

  127. ネオペルトリド蛍光標識アナログの設計・合成・機能評価

    柳 翔太, 野口拓真, 川上雅人, 佐々木 誠, 不破春彦, 丹羽伸介, 杉本 亜砂子

    第１１回日本ケミカルバイオロジー学会　2016/06/15

  128. 線虫C. elegansにおけるチューブリンアイソタイプの発現パターンおよび機能の網羅的解析

    土屋賢汰, 本多優, 杉本亜砂子

    第68回日本細胞生物学会大会　2016/06/15

  129. Spatiotemporal regulation of microtubules during the assembly of mitotic and meiotic spindles International-presentation

    Cell biology, Developmental Genetics, and Stem Cell Biology Seminar Series　2016/04/04

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    Skirball Institute of Biomolecular Medicine, New York University School of Medicine (host: Jane E. Hubbard）

  130. Genetic and molecular tools for Caenorhabditis sp.34, a sister species of C. elegans International-presentation

    Kenji Tsuyama, Satoshi Namai, Ryohei Kumagai, Takuya Shimura, Natsumi Kanzaki, Taisei Kikuchi

    Evolutionary biology of Caenorhabditis and other nematodes　2016/03/30

  131. Distinct microtubule behaviors in zygotes of Caenorhabditis elegans and Pristionchus pacificus International-presentation

    Satoshi Namai, Asako Sugimoto

    Evolutionary biology of Caenorhabditis and other nematodes　2016/03/30

  132. Morphology and ecology of fig fruit nematode Caenorhabditis SP. 34 International-presentation

    Ryuhei Tanaka, Natsumi Kanzaki, Taisei Kikuchi

    Evolutionary biology of Caenorhabditis and other nematodes　2016/03/30

  133. High quality genome sequence of Caenorhabditis SP. 34, the sisuter species of C. elegans International-presentation

    Taisei Kikuchi, Isheng J Tsai, Ryusei Tanaka, Gavin Woodruff, Natsumi Kanzaki

    Evolutionary biology of Caenorhabditis and other nematodes　2016/03/30

  134. ネオペルトリド蛍光標識アナログの合成と機能評価

    柳 翔太, 野口 拓真, 川上 雅人, 佐々木 誠, 不破 春彦, 丹羽 伸介, 杉本 亜砂子

    日本化学会第96春季年会　2016/03/24

  135. MOZART1のγ-チューブリン複合体形成過程への寄与

    春田奈美, 杉本亜砂子

    第33回染色体ワークショップ 第14回核ダイナミクス研究会　2016/01/12

  136. 線虫γ-チューブリン複合体結合因子GTAP-3の 中心小体複製サイクルへの寄与

    内谷進介, 吉井千尋, 住吉英輔, 春田奈美, 久保田幸彦, 寺澤匡博, 杉本亜砂子

    第33回染色体ワークショップ 第14回核ダイナミクス研究会　2016/01/12

  137. 新規足場タンパク質GTAP-3の中心小体複製サイクルへの寄与

    内谷 進介, 住吉 英輔, 春田 奈美, 吉井 千尋, 久保田 幸彦, 寺澤 匡博, 杉本 亜砂子

    第３８回日本分子生物学会年会　2015/12/01

  138. 線虫C. elegansの γ-チューブリン複合体形成におけるMOZART1の関与

    春田 奈美, 杉本 亜砂子

    第３８回日本分子生物学会年会　2015/12/01

  139. 線虫C. elegans新規中心体タンパク質GTAP-3の機能解析

    吉井千尋, 内谷進介, 住吉英輔, 春田奈美, 寺澤匡博, 久保田幸彦, 大山暢仁, 杉本亜砂子

    第３８回日本分子生物学会年会　2015/12/01

  140. 線虫は『進化細胞生物学（Evolutionary Cell Biology）』のモデル系と成り得るか？

    JSTさきがけ「細胞機能の構成的な理解と制御」研究領域第８回領域会議　2015/11/26

  141. 線虫C.elegansとP.pacificusの細胞分裂様式の比較解析

    生井 聡史, 久保田 幸彦, 杉本 亜砂子

    日本動物学会平成 27 年度東北支部大会　2015/08/08

  142. チューブリンアイソタイプの違いがひきおこす微小管ダイナミクスの多様性

    本多優, 住吉英輔, 杉本亜砂子

    第67回日本細胞生物学会大会　2015/06/29

  143. Distinct contribution of different tubulin isotypes to microtubule dynamics. International-presentation

    Yu Honda, Eisuke Sumiyoshi, Asako Sugimoto

    20th International C. elegans Meeting　2015/06/24

  144. Spatiotemporal regulation of microtubules during the assembly of mitotic and meiotic spindles

    HiHA 4th Workship　2015/06/19

  145. 線虫C. elegansを用いた(+)-neopeltolideの作用機序解析

    澁川 哲, 杉本 亜砂子

    第１０回日本ケミカルバイオロジー学会　2015/06/10

  146. 小タンパク質 MOZART1 による線虫γ-チューブリン複合体の制御機構

    春田 奈美, 杉本 亜砂子

    日本生化学会東北支部 第81回例会　2015/05/09

  147. A conserved phosphatase, PPH-4.1, regulates meiotic pairing, synapsis, recombination initiation and crossover formation

    Aya Sato-Carlton, Xuan Li, Asako Sugimoto, Peter Carlton

    第37回 日本分子生物学会年会　2014/11/25

  148. 線虫Aurora BキナーゼAIR-2の染色体依存的微小管形成における機能

    橋間 英一, 住吉 英輔, 春田 奈美, 杉本 亜砂子

    第37回 日本分子生物学会年会　2014/11/25

  149. γ-tubulin複合体構成因子MOZART1のC.elegansオルソログの解析

    春田 奈美, 杉本 亜砂子

    第37回 日本分子生物学会年会　2014/11/25

  150. The nematode C.elegans as a model system for analyzing mechanism of action of chemical compounds

    Akira Shibukawa, Asako Sugimoto

    新学術領域研究「天然物ケミカルバイオロジー」第3回国際シンポジウム　2014/10/28

  151. Unique evolution of γ-tubulin complex in C. elegans and related nematodes. International-presentation

    Asako Sugimoto

    Centrosomes and spindle pole bodies　2014/09/30

  152. Protein phosphatase 4 promotes chromosome pairing and synapsis, and contributes to maintaining crossover competence with increasing age International-presentation

    Aya Sato-Carlton, Xuan Li, Asako Sugimoto, Peter Carlton

    C.elegans Development, Cell Biology and Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting　2014/07/15

  153. Aurora A kinase AIR-1 is required for microtubule assembly of female meiotic spindles International-presentation

    Eisuke Sumiyoshi, Yuma Fukata, Satoshi Namai, Asako Sugimoto

    C.elegans Development, Cell Biology and Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting　2014/07/15

  154. Analyzing the effect of neopeltolide in cell division using C. elegans embryos International-presentation

    Akira Shibukawa, Eisuke Sumiyoshi, Haruhiko Fuwa, Makoto Sasaki, Asako Sugimoto

    C.elegans Development, Cell Biology and Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting　2014/07/15

  155. A novel centrosomal protein GTAP-3 is involved in centriole duplication and the recruitment of γ-tubulin to centrosomes International-presentation

    Shinsuke Uchiya, Eisuke Sumiyoshi, Nami Haruta, Masahiro Terasawa, Nobuhito Oyama, Yukihiko Kubota, Asako Sugimoto

    C.elegans Development, Cell Biology and Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting　2014/07/15

  156. The C. elegans MOZART1 ortholog is essential for the recruitment of the γ-tubulin complex to centrosomes International-presentation

    Nami Haruta, Asako Sugimoto

    C.elegans Development, Cell Biology and Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting　2014/07/15

  157. ZYG-9 contributes to multiple aspects of cell cycle-dependent microtubule behaviors International-presentation

    Satoshi Namai, Eisuke Sumiyoshi, Asako Sugimoto

    C.elegans Development, Cell Biology and Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting　2014/07/15

  158. Analysis of expression pattern, localization and function of the PAF1 complex, which is essential for epidermal morphogenesis in C. elegans International-presentation

    Kenji Tsuyama, Yukihiko Kubota, Yusuke Takabayashi, Nami Haruta, Rika Maruyama, Naoko Iida, Asako Sugimoto

    C.elegans Development, Cell Biology and Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting　2014/07/15

  159. Simple genome editing of essential genes by the CRISPR/Cas9 system using temperature sensitive lethal mutant strains International-presentation

    Yu Honda, Nami Haruta, Yukihiko Kubota, Asako Sugimoto

    C.elegans Development, Cell Biology and Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting　2014/07/15

  160. 線虫C. elegans胚の表皮形態形成に関与する PAF1 複合体の動態解析

    Kenji Tsuyama, Yukihiko Kubota, Yusuke Takabayashi, Rika Maruyama, Naoko Iida, Asako Sugimoto

    日本動物学会平成 26 年度東北支部大会　2014/07/12

  161. Multiple roles of XMAP215/ZYG-9 in controlling microtubule dynamics in C. elegans

    Satoshi Namai, Eisuke Sumiyoshi, Asako Sugimoto

    第36回日本分子生物学会年会　2013/12/03

  162. Expression pattern and interdependent nuclear localization of the components of the PAF1 complex, which is essential for epidermal morphogenesis in C. elegans.

    Kenji Tsuyama, Yukihiko Kubota, Yusuke Takabayashi, Rika Maruyama, Naoko Iida, Asako Sugimoto

    第36回日本分子生物学会年会　2013/12/03

  163. Functional analysis of a new centrosomal protein GTAP-3 in C. elegans

    Shinsuke Uchiya, Eisuke Sumiyoshi, Nami Haruta, Nobuhito Oyama, Masahiro Terasawa, Yukihiko Kubota, Asako Sugimoto

    第36回日本分子生物学会年会　2013/12/03

  164. Contribution of Doublecortin-like kinase ZYG-8 to the assembly of chromatin-stimulated microtubules in C. elegans embryos

    Yuma Fukata, Eisuke Sumiyoshi, Asako Sugimoto

    第36回日本分子生物学会年会　2013/12/03

  165. Contribution of Aurora A to the assembly of centrosome-independent meiotic spindles in C. elegans

    Eisuke Sumiyoshi, Yuma Fukata, Asako Sugimoto

    第36回日本分子生物学会年会　2013/12/03

  166. 線虫C.elegans γ-tubulin複合体の局在および機能制御因子の解析

    春田奈美, 杉本亜砂子

    第31回染色体ワークショップ第12回核ダイナミクス研究会　2013/11/25

  167. 線虫C.elegans γ-tubulin複合体の局在および機能制御因子の解析

    春田奈美, 杉本亜砂子

    第22回DNA複製・組換え・修復ワークショップ　2013/11/20

  168. 受精卵から個体ができるまで¬—卵の中をのぞいてみると・・・

    杉本亜砂子

    第14回生命科学シンポジウム「生命の秘密を解く鍵をもとめて」　2013/11/16

  169. The PAF1 complex is essential for epidermal morphogenesis in C. elegans embryos International-presentation

    Yukihiko Kubota, Yusuke Takabayashi, Kenji Tsuyama, Nami Haruta, Rika Maruyama, Asako Sugimoto

    19th International C. elegans Meeting　2013/06/26

  170. Aurora A is essential for the organization of the female meiotic spindle in late anaphase. International-presentation

    Eisuke Sumiyoshi, Yuma Fukata, Asako Sugimoto

    19th International C. elegans Meeting　2013/06/26

  171. Regulation of localization and function of the γ-tubulin complex at centrosomes

    Asako Sugimoto, Eisuke Sumiyoshi, Nami Haruta, Yu Honda, Masahiro Terasawa, Nobuhito Oyama, Shinsuke Uchiya, Yukihiko Kubota

    第65回日本細胞生物学会大会　2013/06/19

  172. GTAP-3, a new γ-tubulin-associated protein is required for the efficient recruitment of γ-tubulin to centrosomes in C. elegans interphase cells International-presentation

    Nami Haruta, Shinsuke Uchiya, Nobuhito Ohyama, Eisuke Sumiyoshi, Masahiro Terasawa, Yukihiko Kubota, Asako Sugimoto

    The 25th CDB Meeting, Cilia and Centrosomes:from Fertilization to Cancer　2013/06/17

  173. Assembly of female meiotic spindles that mediate extreme asymmetric divisions International-presentation

    Asako Sugimoto

    Cellular polarity: from mechanisms to disease　2013/04/15

  174. GTAP-3, a New γ-Tubulin-Associated Protein is Required for the Efficient Recruitment of γ-Tubulin to Centrosomes in C. elegans Interphase Cells International-presentation

    Nami Haruta, Shinsuke Uchiya, Nobuhito Ohyama, Eisuke Sumiyoshi, Masahiro Terasawa, Yukihiko Kubota

    Swiss-Japanese Developmental Biology Meeting　2012/11/05

  175. The PAF1 complex is essential for epidermal morphogenesis in C. elegans embryos International-presentation

    Yukihiko Kubota, Yusuke Takabayashi, Rika Maruyama

    Morphogenesis and Dynamics of Multicellular Systems　2012/09/07

  176. Analysis of two novel components of the γ-tubulin complex in C. elegans International-presentation

    Yu O. honda, Eisuke Sumiyoshi, Nami Haruta, Masahiro Terasawa, Mika Toya, Yuma Fukata, Yusuke Takabayashi, Yukihiko Kubota

    5th East Asia C. elegans Meeting　2012/06/26

  177. The PAF1 complex is essential for epithelial morphogenesis in C. elegans embryos International-presentation

    Yukihiko Kubota, Yusuke Takabayashi, Rika Maruyama

    5th East Asia C. elegans Meeting　2012/06/26

  178. Involvement of C. elegans Aurora A/AIR-1 in the female meiosis International-presentation

    Yuma Fukata, Eisuke Sumiyoshi

    5th East Asia C. elegans Meeting　2012/06/26

  179. Regulation of cell-cycle dependent behaviors of P granules in C. elegans early embryogenesis International-presentation

    Shin Kamijo, Masafumi Yonetani

    5th East Asia C. elegans Meeting　2012/06/26

  180. Identification of unconventional components of the γ-tubulin complex in C. elegans International-presentation

    Nami Haruta, Eisuke Sumiyoshi, Yu Honda, Masahiro Terasawa, Mika Toya

    C. elegans Meeting 2012　2012/06/07

  181. The Cdc48/p97 cofactor UBXN-2 and its orthologues p47/p37 control centrosome maturation in prophase via Aurora A International-presentation

    Elsa Kress, Francoise Schwager, Rene Holtackers, Esther Zanin, Francois Prodon, Jonas Seiler, Annika Eiteneuer, Hemmo Meyer, Patrick Meraldi, Monica Gotta

    C. elegans Meeting 2012　2012/06/07

  182. Unconventinal gamma-tubulin complex in C. elegans International-presentation

    Eisuke Sumiyoshi, Nami Haruta, Yu Honda, Masahiro Terasawa, Mika Toya

    EMBO Conference Series Microtubules - Structure, Regulation and Functions　2012/05/23

  183. Control of C. elegans gonadogenesis by basement membrane components International-presentation

    Yukihiko kubota, Kayo Nagata, Kiyoji Nishiwaki

    The 3rd UCL-Tohoku University Symposium　2011/09/19

  184. Characterizing the meiotic role of protein phosphatase 4. International-presentation

    Aya Sato, Fumio Motegi, Peter Carlton

    18th International C. elegans Meeting　2011/06/22

  185. Regulation of P granule stability via PGL proteins. International-presentation

    M. Yonetani, M. Hanzawa

    18th International C. elegans Meeting　2011/06/22

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Research Projects 21

  1. Evolutionary cell biology using related nematode species Competitive

    System: Grant-in-Aid for Scientific Research

    2014/04 - Present

  2. Regulation of microtubules in development Competitive

    System: Grant-in-Aid for Scientific Research

    2010/04 - Present

  3. Regulation of assembly and segregation of germ granules Competitive

    System: Grant-in-Aid for Scientific Research

    2010/04 - Present

  4. 線虫特異的な染色体再編成現象を利用した革新的染色体操作技術の開発

    杉本 亜砂子

    Offer Organization: 科学技術振興機構

    System: 戦略的な研究開発の推進 戦略的創造研究推進事業 CREST

    Institution: 東北大学

    2024 - 2029

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    線虫は100万以上の種がいると言われており、その生態や形態、ゲノム構造も極めて多様です。本研究では、発生の過程で染色体の一部が捨てられる「染色体削減」や、種分化と連動した「染色体融合・開裂」などの、一部の線虫でみられるユニークな染色体再編成現象の分子メカニズムを解明します。さらに、明らかにした染色体再編成メカニズムを利用して、他の生物種にも適用できる革新的な染色体操作技術の開発を目指します。

  5. ゲノムスケールのDNA設計・合成による細胞制御技術の創出

    Offer Organization: 国立研究開発法人科学技術振興機構

    System: 平成30年度戦略的想像研究推進事業（CREST)

    Category: 生殖システム進化を駆動するゲノム変化の原理解明と操作

    2018/10 - 2024/03

  6. Analysis of rewiring of genetic pathways by comparison and modification of nematode genomes

    Sugimoto Asako

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2017/07/18 - 2020/03/31

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    In this study, we characterized the rewiring of gene circuits using three nematode species (C. elegans, C. inopinata, and P. pacificus), by comparative genomics and gene function analysis. We focused on the gene circuit rewiring of the establishment of cell polarity and germ granule formation in zygotes. We found that the loss or acquisition of key genes led to the rewiring of gene circuits at a high frequency, even in closely related species. In particular, we found a new phenomenon in the process of cell polarity establishment in P. pacificus zygotes; an actin aggregate is involved in the reorientation of the mitotic spindle, which is not observed in C. elegans.

  7. The roles of the component proteins in the assembly and the recruitment of C. elegans &gamma;-tubulin complex

    HARUTA Nami, SUGIMOTO Asako, UCHIYAMA Chihiro, NAKAJO Momoe, SAITO Yuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2016/04/01 - 2019/03/31

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    The γ-tubulin complex (γTuC) is a widely conserved microtubule (MT) nucleator, which serves as a template for MTs. To understand the molecular mechanism of the assembly and recruitment of γTuC, we characterized several components of γTuC; MOZART1, GTAP-1 and -2. We demonstrated that the interaction between MOZART1 and N-terminus of GIP-1 is essential for the centrosomal recruitment of γTuC, while GTAP-1 and GTAP-2 are not essential but required for efficient recruitment of γTuC. Moreover, GTAP-1 plays a crucial role in germline through controlling the localization of γ-tubulin onto gonad membrane, although GTAP-2 is dispensable. These data strongly indicated that the components of γTuC are likely changed, depending on the cell cycles and types.

  8. Comparative evolutionary study on C. elegans and the sister species

    Kikuchi Taisei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: University of Miyazaki

    2016/04/01 - 2019/03/31

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    A sister species of the model organism Caenorhabditis elegans has long been sought for use in comparative analyses that would enable deep evolutionary. We discovered the first sibling species of C. elegans, C. inopinata, which was isolated from fig syconia in Okinawa. We investigate the morphology, developmental processes and behaviour of C. inopinata, which differ significantly from those of C. elegans. We assembled the 123-Mb C. inopinata genome into six nuclear chromosomes and revealed unique characteristics, such as highly expanded transposable elements and massive gene losses in chemoreceptor gene families. We have developed genetic and molecular techniques for C. inopinata; thus C. inopinata provides an exciting new platform for comparative evolutionary studies.

  9. Traits changing associated with the evolution of body size

    KAWATA MASAKADO, SUGIMOTO Asako, MAKINO Takashi, MARUYAMA Shinichro, YOKOYAMA Jun

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2016/04/01 - 2018/03/31

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    We purpose to determine candidate genes responsible for the evolution of larger body size of C. inopinata. C. inopinata became larger during developmental L4 to adult stages comparing with C. elegans. Then, we detected 2699 genes which expression patterns differed between C. inopinata and C. elegans. We also detected 42 genes which have been subject to positive selection during the evolution of C. inopinata from other related species. Among them, daf-2 could be detected and this gene encodes the insulin-like growth factor 1 receptor and variant of def-1 in C. elegance became larger. Thus, the results suggest that daf-2 is as an important genes affecting the evolution of large body size of C. inopinata

  10. Revisiting "the multi-tubulin hypothesis" through comprehensive analysis of tubulin isotypes

    Sugimoto Asako, KUBOTA Yukihiko, HARUTA Nami, HONDA Yu, TSUCHIYA Kenta, OBINATA Hiroyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    In this study, we tested the "multi-tubulin hypothesis" in which multiple tubulin isotypes contribute to the diversity of microtubule dynamics, using the nematode C. elegans as a model system. Using genome editing, all tubulin isotype genes (9 alpha and 6 beta genes) were systematically knocked out and tagged with GFP, and their expression patterns and loss-of-function phenotypes were analyzed. Each isotypes were expressed in specific cell types, and ectopic expression experiments proved that composition of tubulin isotypes contribute to the diversity of microtubule dynamics.

  11. Nematodes as a model system for studying evolution of diverse microtubule behaviors

    Sugimoto Asako, KUBOTA Yukihiko, HONDA Yu, NAMAI Satoshi, TSUYAMA Kenji, TSUCHIYA Kenta, KUMAGAI Ryohei, OBINATA Hiroyuki, SASAKI Daichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2017/03/31

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    In this study, we established a model system for “evolutionary cell biology” using nematode Caenorhabditis elegans and Pristionchus pacificus. By live imaging of microtubules and actin filaments, we found that the timing of microtubule assembly/disassembly is different in these two nematodes, which correlates with distinct mitotic spindle behaviors and cell polarity establishment mechanisms.

  12. 線虫胚を用いた天然物リガンドの作用機序解明および標的因子探索法の構築

    杉本 亜砂子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東北大学

    2014/04/01 - 2016/03/31

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    本研究では、C. elegans初期胚をモデル系として用いて、細胞増殖抑制活性を持つ天然化合物(+)-ネオペルトリドの作用機序解析を行った。(+)-ネオペルトリドはin vitroでシトクロムbc1複合体活性を阻害することが報告されていたが、これが直接細胞増殖抑制を引き起こしているのかは不明であった。染色体・微小管・中心体を蛍光タンパク質で標識したC. elegans受精卵を(+)-ネオペルトリドで暴露し、その表現型をライブイメージングによって解析した結果、細胞分裂が停止し、紡錘体微小管（とくに、動原体微小管）が過度に伸長することを見出した。阻害剤やRNAiによってシトクロムbc1を阻害しても微小管の過剰伸長は見られなかったことから、(+)-ネオペルトリドの標的はシトクロムbc1にも存在することが示唆された。蛍光標識ネオペルトリド類縁体（東北大・不破らより供与）を線虫胚に導入し、その集積場所を顕微鏡観察によって調べた結果、小胞体に集積することが示された。以上より、(+)-ネオペルトリドは線虫胚において、小胞体に局在する分子を標的とし、微小管の安定化を引き起こしていることが示唆された。

  13. Function of the novel tumor suppressor in cell division and carcinogenesis

    Chiba Natsuko, WATABANE Toshio, KANNO Shin-ichiro, Sugimoto Asako

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2012/04/01 - 2016/03/31

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    The breast and ovarian cancer specific tumor suppressor BRCA1 plays important roles in DNA repair and centrosome regulation. We identified Obg-like ATPase 1 (OLA1) that interacts with BRCA1. OLA1 directly bound to BRCA1 and a component of centrosome. OLA1 localized to centrosomes in interphase and to the spindle pole in mitotic phase, and its knockdown resulted centrosome amplification and the activation of microtubule aster formation. OLA1 with a mutation observed in breast cancer cell line failed to bind BRCA1 and rescue the OLA1 knockdown-induced centrosome amplification. BRCA1 variant found in familial breast cancer also abrogated the binding of BRCA1 to OLA1. These findings suggest that OLA1 plays an important role in centrosome regulation together with BRCA1.

  14. Analysis of genetic networks that regulate cytoskeletal dynamics in animal development

    SUGIMOTO Asako

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (S)

    2007 - 2011

  15. Analysis of developmental mechanisms based on functional genomic information of the nematode C. elegans

    SUGIMOTO Asako, ONAMI Shuichi, KIMURA Akatsuki, KYODA Koji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Priority Areas

    Institution: The Institute of Physical and Chemical Research

    2005 - 2009

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    To understand the genetic networks that control animal development, the following were performed using the early embryogenesis of the nematode C.elegans as a model system. 1) Development of tools (such as GFP/RFP-tagged protein markers and monoclonal antibodies) and imaging techniques, and imaging data collection, 2) Computational analysis of the phenotype image data, and 3) Development of research strategy that integrate quantitative phenotype analysis and simulation.

  16. Functional analysis of the C. elegansgenome by a systematic gene knock-down

    SUGIMOTO Asako, MITANI Shohei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Priority Areas

    Institution: RIKEN

    2003 - 2004

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    In this study, we aimed to understand the framework of genetic cascades and networks that regulate the development and behavior of multicellular organisms, using the nematode C. elegans as a model system. To this end, we performed systematic analyses of loss-of-function phenotypes caused by either RNAi or deletion mutations. 1. A general phenotype analysis was performed for〜6000 genes by an RNAi-by-soaking method, and 30% of them were shown to be essential for developmental processes. We further performed embryonic phenotype profiling of the 833 embryonic lethal genes identified in this screen. A cluster analysis of the phenotypic profiles successfully grouped the gene sets that were known to function in the same developmental/biological processes. Thus, this method will be useful to predict gene functions of uncharacterized genes. We compiled these data and established "A C. elegans RNAi phenome database" accessible via Web. We also performed the post-embryonic functions of the embryonic lethal genes by a developmental stage-specific RNAi method. 2. Systematic screening for deletion mutants of transcription factors in C. elegans was conducted. We have isolated mutants corresponding to 437 genes, which are more than two thirds of whole transcription factors. We are also isolating many other mutants and simultaneously use them to reveal gene function. Among the mutants, we found that ags-3 mutants show abnormal chemosensory perception, body size and moving activity. We found that ags-3 phenotypes are suppressed by an egl-4 (PKG) mutant. AGS-3 is known to play an important role in cocaine addiction. We found that AGS-3 and PKG from both nematode and mouse interact with each other.

  17. Molecular Mechanisms for the Regulation of Meiosis

    YAMAMOTO Masayuki, TANAKA Kayoko, KARASHIMA Takeshi, WATANABE Yoshinori, YAMASHITA Akira

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Specially Promoted Research

    Institution: The University of Tokyo

    1999 - 2003

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    1.We screened for suppressors of the fission yeast meiosis-defective mutant lacking meiRNA, which is indispensable for meiosis I, and isolated meiosis-specific genes mei4, spo5, ssm4 and others. It turned out that the suppression was caused not by their protein products but by part of their mRNAs. Interestingly, these mRNAs were unstable and did not accumulate in mitotically growing cells, even if artificially expressed, and the region responsible for this instability overlapped with the region that suppressed loss of meiRNA. Thus we speculate that these mRNAs carry a region that renders them unstable in the mitotic cell cycle, and that a certain mechanism operates to stabilize them during meiosis. 2.Fission yeast Mei2p, the regulator of the initiation of meiosis, forms a dot structure in prophase I nuclei. We have demonstrated that this dot is a complex of Mei2p and nascent meiRNA, just transcribed from the gene. 3.We isolate a new mutant of fission yeast tor1, which encodes a TOR kinase required for sexual development. We then isolated gad8, which encoded a kinase of the AGC family, as a suppressor of this mutant. Analysis has shown that Tor1p regulates sexual development and stress responses through Gad8p. 4.We isolated C. elegans DAZ-1, a homolog of DAZ responsible for azoospermia in mammals. In DAZ-1-defective worms, the meiotic process was arrested at pachytene during oogenesis. They showed abnormal nuclear morphology even in germ cells at the proliferative stage in the gonad. The DAZ-1-defective worms did not show expansion of nucleoli nor formation of the central cytoplasmic core in germ cells at the meiotic stage, and these cells appeared to be less active in protein synthesis. Other data have suggested that DAZ-1 may also be involved in switching sexuality of the gonad.

  18. 体系的RNAiによる線虫C.elegansのゲノム機能解析

    杉本 亜砂子, 作本 直子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 特定領域研究

    Institution: 特殊法人理化学研究所

    2002 - 2002

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    1.体系的RNAi法のさらなるハイスループット化 体系的RNAiをさらに効率的に行うために、従来はマニュアルで行っていた二本鎖RNAの合成過程を自動化するための条件決定を行った。具体的には、ラボラトリーオートメーションシステム(Biomek)を用いて、96穴プレートフォーマットで非重複cDNAセットから高濃度の二本鎖RNAを合成する条件を決定した。 2.体系的RNAi法による必須遺伝子の網羅的探索 約10,000遺伝子由来のcDNAを含む非重複cDNAセット(国立遺伝研・小原雄治教授より供与)を鋳型として、上述の手法により二本鎖RNAを合成し、線虫を高濃度RNA溶液に浸すことによって体系的なRNAiを行った。各cDNAに対するRNAiによる表現型を実体顕微鏡で観察し、表現型を記録した。表現型としては、胚性致死・幼生致死・不稔・形態異常・運動能力異常等に着目し、表現型データベースを並行して構築した。平成12月現在で、約4500クローンについての解析を完了した。 3.胚性致死遺伝子群の分類 上記の体系的RNAiで胚性致死となったものについて最終表現型を微分干渉顕微鏡で観察し、発生停止時期(細胞増殖期、形態形成期、形態形成完了後、等)によって分類を行った。また、神経・筋肉・腸・咽頭等の細胞分化や組織形成についても記録した。これまでに、約120の胚性致死遺伝子についての解析を完了した。これらの表現型解析に基づき、形態形成過程に特異的に異常のあるものを選択して以後の解析を行う予定である。

  19. Molecular mechanisms of germline development in the nematode C. elegans

    SUGIMOTO Asako

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: The University of Tokyo

    2000 - 2001

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    Previously we established a novel high-throughput reverse genetic method ("RNAi-by-soaking") to systematically characterize gene functions in the nematode C. elegans. Using this technique, we have screened for gene involved in germline development. We used a non-redundant CDNA set made by Yuji Kohara, as templates to synthesize double-stranded RNA. To date, we have analyzed the RNAi phenotypes for 2800 cDNAs, and 31 of those caused germline-specific defects and became sterile adults. The germline phenotypes included defects in germline proliferation, oogenesis, gonadogenesis, and fertilization. Thus, this screen effectively identified genes involved in various steps in germline development. Among the genes identified in this screen, we have further characterized a gene, hmg-3, that has a HMG box, and found that it is involved in germline proliferation. We also performed RNAi experiments for the hmg-4 gene that shares a high homology with hmg-3, which resulted in larval lethality. In addition, RNAi for both hmg-3 and hmg-4 caused embryonic lethality. These results suggest that hmg-3 and hmg-4 have redundant functions during embryogenesis, and in post-embryonic development, they play distinct roles in germline and somatic development, respectively.

  20. 線虫C．elegansにおける生殖細胞分化の分子機構

    杉本 亜砂子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東京大学

    1999 - 1999

  21. 線虫C.elegansの新しい細胞死関連遺伝子の検索と解析

    杉本 亜砂子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 奨励研究(A)

    Institution: 東京大学

    1997 - 1998

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    本研究では線虫C.elegansのプログラム細胞死に関与する新しい遺伝子の分離・解析を目的として、細胞死異常を示す染色体欠失の解析を行った。 前年度までに行ったスクリーニングにより、細胞死異常が引き起こされる約10の染色体欠失を同定した。そのなかで、異常に大きな死細胞を呈するeDf2に着目してさらなる解析を行った。eDf2は第III染色体の約1/3にわたる広い領域を欠損している。そこで、巨大な死細胞を引き起こす原因遺伝子の存在領域を狭めるため、eDf2とオーバーラップする染色体欠失に面して表現型を解析した。その結果、第3染色体右端の約2map unitの領域に原因遺伝子が存在することが明らかになった。以後、該当領域を欠失する最小の染色体欠失であるtDf6を用いて解析を行った。 tDf6に見られる巨大な死細胞が実際にプログラム細胞死経路によって生じているか否かを明らかにするため、C.elegannsの既知の細胞死変異体とtDf6との二重変異体の解析を行った。細胞死が全く起きなくなるced-3変異とtDf6との二重変異体では通常の死細胞に加え巨大な死細胞も見られなくなった。したがって、tDf6に見られる巨大な死細胞は通常のプログラム細胞死経路によって死んだ細胞であることが示された。また、tDf6の細胞数は野生型と有為な差がなかったため、巨大な死細胞が細胞分裂早期停止に起因するものではないことが示された。 今後は、tDf6の巨大な死細胞がどの細胞の死によるものなのか、どのように形成されるかを明らかにするために、胚発生過程を録画し経時的な解析を行う予定である。また、併行して原因遺伝子のクローニングを進めている。

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  1. サイエンス・デイ

    2012/07/10 -

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    講座プログラム開催

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    2011/07/10 -

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    講座プログラム開催

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  1. 体細胞分裂と雌性減数分裂における紡錘体構造の違いをもたらす機構の解明

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    体細胞分裂と雌性減数分裂における紡錘体構造の違いをもたらす機構の解明

  2. 卵細胞における中心体非依存的な紡錘体構築メカニズムの解明

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    卵細胞における中心体非依存的な紡錘体構築メカニズムの解明

  3. 胚発生過程における細胞の極性と形態の時空間的制御メカニズム

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    からだの構造がシンプルな線虫の胚発生をモデル系として、細胞の分裂・形態変化・移動を引き起こすために、ゲノムに含まれる遺伝子群がどのように協調的に働いているかを明らかにします。

  4. 細胞分裂期における二つの微小管形成機構の時空間的制御メカニズム

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    微小管は細胞周期にしたがってダイナミックな制御を受け、細胞分裂期になると特殊な微小管構造である紡錘体を形成する。分裂期の主要な微小管形成中心は中心体であるが、凝縮した染色体も微小管形成能を持つことが知られている。紡錘体構築においては、これら二つの「場」での微小管形成が時空間的に厳密に制御されている必要があるが、その分子メカニズムについては不明な点が多い。これまでに我々は線虫初期胚において従来から知られていたγ−チューブリン（TGB-1）依存的な微小管（TGB-1微小管）に加えて、Aurora Aキナーゼ（AIR-1）を必要とするγ−チューブリン非依存的微小管（AIR-1微小管）が存在することを新たに発見し（Motegi, et al., Dev Cell, 2006）、その解析をすすめてきた。ライブイメージングとRNAiによる遺伝子機能破壊解析によって、線虫初期胚の紡錘体形成には両方の微小管形成経路の寄与が必要であり、染色体依存的微小管に寄与しているのはAIR-1微小管のみであることを見いだした。本研究では、我々が見いだしたAIR-1微小管の形成機構を明らかにするとともに、TGB-1微小管とAIR-1微小管が強調して紡錘体を構築するための時間的・空間的制御機構を解明することをめざす。

  5. 紡錘体構築における微小管形成の時空間的制御メカニズム

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    微小管は細胞周期にしたがってダイナミックな制御を受け、細胞分裂期になると特殊な微小管構造である紡錘体を形成する。分裂期の主要な微小管形成中心は中心体であるが、凝縮した染色体も微小管形成能を持つことが知られている。紡錘体構築においては、これら二つの「場」での微小管形成が時空間的に厳密に制御されている必要があるが、その分子メカニズムについては不明な点が多い。これまでに我々は線虫初期胚において従来から知られていたγ−チューブリン（TGB-1）依存的な微小管（TGB-1微小管）に加えて、Aurora Aキナーゼ（AIR-1）を必要とするγ−チューブリン非依存的微小管（AIR-1微小管）が存在することを新たに発見し（Motegi, et al., Dev Cell, 2006）、その解析をすすめてきた。ライブイメージングとRNAiによる遺伝子機能破壊解析によって、線虫初期胚の紡錘体形成には両方の微小管形成経路の寄与が必要であり、染色体依存的微小管に寄与しているのはAIR-1微小管のみであることを見いだした。本研究では、我々が見いだしたAIR-1微小管の形成機構を明らかにするとともに、TGB-1微小管とAIR-1微小管が強調して紡錘体を構築するための時間的・空間的制御機構を解明することをめざす。