Details of the Researcher

PHOTO

Koji Okamoto
Section
Tohoku University Hospital
Job title
Senior Assistant Professor
Degree

  • 博士（医学）（東京大学）

e-Rad No.
80572247

Research History 11

  • 2021/04 - Present
    Tohoku University　Division of Nephrology and Vascular Medicine　Senior lecturer

  • 2019/04 - 2021/03
    東北メディカル・メガバンク機構　東北メディカル・メガバンク機構　助教

  • 2018/10 - 2019/03
    Tohoku University Hospital　Division of Nephrology, Nephrology and Vascular Medicine　Assistant professor

  • 2012/04 - 2018/03
    Naitional institute of Health　National Institute of Diabetes and Digestive and Kidney Diseases　Visiting Fellow

  • 2012/06 - 2014/05
    Japan Society for the Promotion of Science　Overseas Research Fellowships

  • 2011/04 - 2012/03
    The University Tokyo Hospital　Department of Nephrology and Endocrinology　Assistant professor

  • 2009/04 - 2010/03
    The University Tokyo Hospital　Department of Nephrology and Endocrinology　Postdoctoral Fellow

  • 2003/04 - 2005/03
    Shonan Kamakura General Hospital　Senior Resident in Nephrology

  • 2002/04 - 2003/03
    Japanese Red Cross Medical Center　Internal Medicine　Junior Resident

  • 2001/04 - 2002/03
    The University Tokyo Hospital　Internal Medicine　Junior Resident

  • 2022/04 -
    Tohoku University Hospital　Division of Blood purification　Vice director

Show all Show first 5

Education 2

  • University of Tokyo　Faculty of Medicine　Internal Medicine

    2005/04 - 2009/03

  • Tohoku University　School of Medicine

    1995/04 - 2001/03

Professional Memberships 3

  • THE JAPAN SOCIETY OF HUMAN GENETICS

  • THE JAPANESE SOCIETY FOR DIALYSIS THERAPY

  • JAPANESE SOCIETY OF NEPHROLOGY

Research Interests 6

  • Atherosclerosis

  • Disease susceptibility gene

  • HIV associated nephropathy

  • Diabetic nephropathy

  • RNA structure

  • Nephrotic syndrome

Research Areas 4

  • Life sciences / Virology / Acquired immunodeficiency syndrome

  • Life sciences / Medical biochemistry / Disease susceptibility gene

  • Life sciences / Metabolism and endocrinology / Diabetes mellitus

  • Life sciences / Nephrology / Glomerular disease

Awards 3

  1. JSN Best Paper Award

    2021/06　Genome-wide association study identifies new loci for albuminuria in the Japanese population

  2. 23rd Annual Fellows Research Forum Award

    2014/06　National Kidney Foundation

  3. CKD Award 2011

    2011/11　Boehringer Ingelheim GmbH

Papers 72

  1. Influence of infection control for COVID-19 on nutrition in relatively healthy Japanese HD patients: a retrospective observational study.

    Yuki Chiba, Ryotaro Takahashi, Rui Makino, Mai Yoshida, Koji Okamoto, Tasuku Nagasawa, Ichiro Kato, Sadatoshi Ito, Tetsuhiro Tanaka, Mariko Miyazaki

    Clinical and experimental nephrology　2025/02/28

    DOI： 10.1007/s10157-025-02638-3 　

    More details Close

    BACKGROUND: Infection control for the novel coronavirus disease 2019 (COVID-19) has been linked to decreased physical activity and nutritional deterioration in the general population; however, the influence on hemodialysis (HD) patients is not well discussed. METHODS: This multicenter retrospective study utilized the Geriatric Nutritional Risk Index (GNRI), Survival Index, and Nutritional Risk Index for Japanese HD patients (NRI-JH) to assess nutritional status and body composition over five observation periods. The primary endpoint was the body fluid removal rate (%) pre- and post-HD, whereas secondary endpoints included changes in GNRI, SI, body composition, and differences in NRI-JH. RESULTS: We enrolled 139 HD patients in three facilities. The results showed a decrease in GNRI score, which indicates nutritional deterioration, between February 2020 and August 2020 (96.8 (93.2-98.9) vs. 93.8 (90.8-97.6)) (P = 0.0005). Multivariable analysis revealed that nutritional deterioration was associated with higher C-reactive protein and lower hemoglobin levels (P = 0.0004 and P = 0.0010, respectively), which were more noticeable in the urban facility. Furthermore, nutritional deterioration was linked to a decrease in soft lean and somatic cell mass and an increase in body fat mass, suggesting reduced physical activity. CONCLUSIONS: Nutritional deterioration was observed shortly after the first COVID-19 outbreak, suggesting an association with decreased physical activity.

  2. Waldenström's Macroglobulinemia/Lymphoplasmacytic Lymphoma Developing Renal AA Amyloidosis: A Case Report and Literature Review.

    Yusuke Ishizuka, Yuji Oe, Sosuke Kinomura, Saori Kin, Yuji Noguchi, Koichi Kikuchi, Mai Yoshida, Rui Makino, Koji Okamoto, Tasuku Nagasawa, Takafumi Toyohara, Mariko Miyazaki, Hiroshi Sato, Yasushi Onishi, Hitoshi Warita, Tetsuhiro Tanaka

    Internal medicine (Tokyo, Japan)　2025/02/08

    DOI： 10.2169/internalmedicine.4678-24 　

    More details Close

    AA amyloidosis is a rare renal complication of Waldenström's macroglobulinemia/lymphoplasmacytic lymphoma (WM/LPL). A 66-year-old man with WM/LPL presented with nephrotic syndrome. A renal biopsy showed AA amyloidosis. Chemotherapy resulted in the remission of hematologic and nephrotic syndromes. Two years into follow-up, he became infected with COVID-19 and had massive proteinuria, despite no relapse of WM/LPL. A second renal biopsy confirmed a diagnosis of AA amyloidosis. However, increased prednisolone did not improve proteinuria. The patient ultimately died of cryptococcal meningitis. This case highlights the diverse spectrum of renal involvement in monoclonal IgM-secreting diseases and difficulty in managing fatal complications.

  3. Deep learning-based histopathological assessment of tubulo-interstitial injury in chronic kidney diseases. International-journal

    Nonoka Suzuki, Kaname Kojima, Silvia Malvica, Kenshi Yamasaki, Yoichiro Chikamatsu, Yuji Oe, Tasuku Nagasawa, Ekyu Kondo, Satoru Sanada, Setsuya Aiba, Hiroshi Sato, Mariko Miyazaki, Sadayoshi Ito, Mitsuhiro Sato, Tetsuhiro Tanaka, Kengo Kinoshita, Yoshihide Asano, Avi Z Rosenberg, Koji Okamoto, Kosuke Shido

    Communications medicine　5　(1)　3-3　2025/01/05

    DOI： 10.1038/s43856-024-00708-3 　

    More details Close

    BACKGROUND: Chronic kidney disease (CKD) causes progressive and irreversible damage to the kidneys. Renal biopsies are essential for diagnosing the etiology and prognosis of CKD, while accurate quantification of tubulo-interstitial injuries from whole slide images (WSIs) of renal biopsy specimens is challenging with visual inspection alone. METHODS: We develop a deep learning-based method named DLRS to quantify interstitial fibrosis and inflammatory cell infiltration as tubulo-interstitial injury scores, from WSIs of renal biopsy specimens. DLRS segments WSIs into non-tissue areas, glomeruli, tubules, interstitium, and arteries, and detects interstitial nuclei. It then quantifies these tubulo-interstitial injury scores using the segmented tissues and detected nuclei. RESULTS: Applied to WSIs from 71 Japanese CKD patients with diabetic nephropathy or benign nephrosclerosis, DLRS-derived scores show concordance with nephrologists' evaluations. Notably, the DLRS-derived fibrosis score has a higher correlation with the estimated glomerular filtration rate (eGFR) at biopsy than scores from nephrologists' evaluations. Validated on WSIs from 28 Japanese tubulointerstitial nephritis patients and 49 European-ancestry patients with nephrosclerosis, DLRS-derived scores show a significant correlation with eGFR. In an expanded analysis of 238 Japanese CKD patients, including 167 from another hospital, deviations in eGFR from expected values based on DLRS-derived scores correlate with annual eGFR decline after biopsy. Inclusion of these deviations and DLRS-derived fibrosis scores improve predictions of the annual eGFR decline. CONCLUSIONS: DLRS-derived tubulo-interstitial injury scores are concordant with nephrologists' evaluations and correlated with eGFR across different populations and institutions. The effectiveness of DLRS-derived scores for predicting annual eGFR decline highlights the potential of DLRS as a predictor of renal prognosis.

  4. The polymyxin-B direct hemoperfusion OPTimal Initiation timing with Catecholamine PMX-OPTIC study: A multicenter retrospective observational study. International-journal

    Kensuke Nakamura, Tetsuya Okazaki, Akihito Tampo, Katsunori Mochizuki, Naoki Kanda, Takahiro Ono, Kunio Yanagita, Taro Shimomura, Taichi Murase, Ken Saito, Takahiro Hirayama, Tomoaki Ito, Koji Ogawa, Mizuki Nakamura, Tomohiro Oda, Takeshi Morishima, Takuma Fukushima, Hiroharu Yasui, Naoki Akashi, Kojiro Oshima, Hiroo Kawarazaki, Tsukasa Akiba, Susumu Uemura, Yuhei Honma, Kenichi Nitta, Koji Okamoto, Shunsuke Takaki, Hirotaka Takeda, Chizuru Yamashita

    Artificial organs　2024/09/18

    DOI： 10.1111/aor.14865 　

    More details Close

    BACKGROUND: Polymyxin-B direct hemoperfusion (PMX-DHP) is an endotoxin adsorption column-based blood purification therapy. Since one of the most potent effects of PMX-DHP is blood pressure elevations, it may be the most effective when it is introduced at the time when the need for vasopressors is the greatest, which, in turn, may reduce mortality. METHODS: A multicenter retrospective study was conducted at 24 ICUs in Japan. In each ICU, the 20 most recent consecutive cases of septic shock treated with PMX-DHP were analyzed. The duration between the time of the peak vasopressive agent dose, expressed as the noradrenaline equivalent dose (NEq), and the time of PMX initiation was evaluated. The primary outcome was 28-day mortality, and a multivariable analysis was performed to investigate factors associated with mortality. RESULTS: A total of 480 septic shock patients were included in the analysis. Among all patients, the 28-day mortality group was older, more severely ill, and had a higher body mass index. The NEq peak and NEq on PMX-DHP initiation were both higher in deceased patients. Regarding the timing of PMX-DHP initiation from the NEq peak, -4 << 4 h had more survivors (229/304, 75.3%) than ≤-4 h (50/75, 66.7%) and ≥4 h (66/101, 65.4%) (p = 0.085). When -4 << 4 h was assigned as a reference, the timing of PMX-DHP initiation from the NEq peak of ≤-4 h had an odds ratio of 1.96 (1.07-3.58), p = 0.029, while ≥4 h had an odds ratio of 1.64 (0.94-2.87), p = 0.082 for 28-day mortality, in the multivariable regression analysis. A spline curve of the relationship between the probability of death and the timing of PMX-DHP initiation from the NEq peak showed a downward convex curve with a nadir at timing = 0. The odds ratios of the timing of PMX-DHP initiation other than -4 << 4 h were significantly higher in an older age, male sex, lower BMI, more severe illness, and higher oxygenation. CONCLUSIONS: The induction of PMX-DHP at the time of the peak vasopressor dose correlated with lower mortality. PMX-DHP is one of the options available for elevating blood pressure in septic shock, and its initiation either too early or late for shock peak may not improve the outcome.

  5. 分娩後に劇症型A群レンサ球菌感染症による血栓性微小血管症(TMA)を合併した一例

    金銅 妃奈子, 岡本 好司, 秋保 真穂, 濱田 裕貴, 只川 まり, 岩間 憲之, 齋藤 昌利, 田中 哲洋, 宮崎 真理子

    日本腎臓学会誌　66　(6-E)　883-883　2024/09

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  6. 分娩時大量出血により発症した妊娠関連血栓性微小血管症(TMA)の一例

    吉田 舞, 石塚 悠奨, 木之村 聡介, 野口 雄司, 金 沙織, 菊地 晃一, 大江 佑治, 岡本 好司, 豊原 敬文, 宮崎 真理子, 田中 哲洋

    日本腎臓学会誌　66　(6-E)　884-884　2024/09

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  7. 溶血性尿毒症症候群に伴う意識障害が遷延した一例

    野口 雄司, 豊原 敬文, 石塚 悠奨, 木之村 聡介, 金 沙織, 菊地 晃一, 吉田 舞, 牧野 塁, 岡本 好司, 長澤 将, 宮崎 真理子, 田中 哲洋

    日本腎臓学会誌　66　(6-E)　913-913　2024/09

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  8. 非典型的な臨床経過と腎病理を呈した多発血管炎性肉芽腫症

    木之村 聡介, 豊原 敬文, 白井 剛志, 秋保 真穂, 菊地 晃一, 牧野 塁, 吉田 舞, 大江 佑治, 岡本 好司, 長澤 将, 宮崎 真理子, 佐藤 博, 藤井 博司, 田中 哲洋

    日本腎臓学会誌　66　(6-E)　957-957　2024/09

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  9. PKR activation-induced mitochondrial dysfunction in HIV-transgenic mice with nephropathy. International-journal

    Teruhiko Yoshida, Khun Zaw Latt, Avi Z Rosenberg, Briana A Santo, Komuraiah Myakala, Yu Ishimoto, Yongmei Zhao, Shashi Shrivastav, Bryce A Jones, Xiaoping Yang, Xiaoxin X Wang, Vincent M Tutino, Pinaki Sarder, Moshe Levi, Koji Okamoto, Cheryl A Winkler, Jeffrey B Kopp

    eLife　12　2024/08/29

    DOI： 10.7554/eLife.91260 　

    More details Close

    HIV disease remains prevalent in the USA and chronic kidney disease remains a major cause of morbidity in HIV-1-positive patients. Host double-stranded RNA (dsRNA)-activated protein kinase (PKR) is a sensor for viral dsRNA, including HIV-1. We show that PKR inhibition by compound C16 ameliorates the HIV-associated nephropathy (HIVAN) kidney phenotype in the Tg26 transgenic mouse model, with reversal of mitochondrial dysfunction. Combined analysis of single-nucleus RNA-seq and bulk RNA-seq data revealed that oxidative phosphorylation was one of the most downregulated pathways and identified signal transducer and activator of transcription (STAT3) as a potential mediating factor. We identified in Tg26 mice a novel proximal tubular cell cluster enriched in mitochondrial transcripts. Podocytes showed high levels of HIV-1 gene expression and dysregulation of cytoskeleton-related genes, and these cells dedifferentiated. In injured proximal tubules, cell-cell interaction analysis indicated activation of the pro-fibrogenic PKR-STAT3-platelet-derived growth factor (PDGF)-D pathway. These findings suggest that PKR inhibition and mitochondrial rescue are potential novel therapeutic approaches for HIVAN.

  10. Single-Nucleus RNA Sequencing Reveals Loss of Distal Convoluted Tubule 1 Renal Tubules in HIV Viral Protein R Transgenic Mice. International-journal

    Khun Zaw Latt, Teruhiko Yoshida, Shashi Shrivastav, Amin Abedini, Jeff M Reece, Zeguo Sun, Hewang Lee, Koji Okamoto, Pradeep Dagur, Yu Ishimoto, Jurgen Heymann, Yongmei Zhao, Joon-Yong Chung, Stephen Hewitt, Pedro A Jose, Kyung Lee, John Cijiang He, Cheryl A Winkler, Mark A Knepper, Tomoshige Kino, Avi Z Rosenberg, Katalin Susztak, Jeffrey B Kopp

    The American journal of pathology　194　(10)　1844-1856　2024/07/18

    DOI： 10.1016/j.ajpath.2024.06.006 　

    More details Close

    Although hyponatremia and salt wasting are common in patients with HIV/AIDS, the understanding of their contributing factors is limited. HIV viral protein R (Vpr) contributes to HIV-associated nephropathy. To investigate the effects of Vpr on the distal tubules and on the expression level of the Slc12a3 gene, encoding the sodium-chloride cotransporter (which is responsible for sodium reabsorption in distal nephron segments), single-nucleus RNA sequencing was performed on kidney cortices from three wild-type (WT) and three Vpr transgenic (Vpr Tg) mice. The results show that the percentage of distal convoluted tubule (DCT) cells was significantly lower in Vpr Tg mice compared with WT mice (P < 0.05); in Vpr Tg mice, Slc12a3 expression was not significantly different in DCT cells. The Pvalb+ DCT1 subcluster had fewer cells in Vpr Tg mice compared with WT mice (P < 0.01). Immunohistochemistry revealed fewer Slc12a3+Pvalb+ DCT1 segments in Vpr Tg mice. Differential gene expression analysis between Vpr Tg and WT samples in the DCT cluster showed down-regulation of the Ier3 gene, which is an inhibitor of apoptosis. The in vitro knockdown of Ier3 by siRNA transfection induced apoptosis in mouse DCT cells. These observations suggest that the salt-wasting effect of Vpr in Vpr Tg mice is likely mediated by Ier3 down-regulation in DCT1 cells and loss of Slc12a3+Pvalb+ DCT1 segments.

  11. HIV viral protein R induces loss of DCT1-type renal tubules. International-journal

    Khun Zaw Latt, Teruhiko Yoshida, Shashi Shrivastav, Amin Abedini, Jeff M Reece, Zeguo Sun, Hewang Lee, Koji Okamoto, Pradeep Dagur, Jurgen Heymann, Yongmei Zhao, Joon-Yong Chung, Stephen Hewitt, Pedro A Jose, Kyung Lee, John Cijiang He, Cheryl A Winkler, Mark A Knepper, Tomoshige Kino, Avi Z Rosenberg, Katalin Susztak, Jeffrey B Kopp

    bioRxiv : the preprint server for biology　2023/11/13

    DOI： 10.1101/2023.02.02.526686 　

    More details Close

    Hyponatremia and salt wasting is a common occurance in patients with HIV/AIDS, however, the understanding of its contributing factors is limited. HIV viral protein R (Vpr) contributes to HIV-associated nephropathy. To investigate the effects of Vpr on the expression level of the Slc12a3 gene, encoding the Na-Cl cotransporter, which is responsible for sodium reabsorption in distal nephron segments, we performed single-nucleus RNA sequencing of kidney cortices from three wild-type (WT) and three Vpr-transgenic (Vpr Tg) mice. The results showed that the percentage of distal convoluted tubule (DCT) cells was significantly lower in Vpr Tg mice compared with WT mice (P < 0.05), and that in Vpr Tg mice, Slc12a3 expression was not different in DCT cell cluster. The Pvalb+ DCT1 subcluster had fewer cells in Vpr Tg mice compared with WT (P < 0.01). Immunohistochemistry demonstrated fewer Slc12a3+ Pvalb+ DCT1 segments in Vpr Tg mice. Differential gene expression analysis comparing Vpr Tg and WT in the DCT cluster showed Ier3, an inhibitor of apoptosis, to be the most downregulated gene. These observations demonstrate that the salt-wasting effect of Vpr in Vpr Tg mice is mediated by loss of Slc12a3+ Pvalb+ DCT1 segments via apoptosis dysregulation.

  12. 東北大学病院における透析非導入症例の緩和ケアの現状

    藤倉 恵美, 岡本 好司, 吉田 舞, 宮崎 真理子, 田中 哲洋

    日本腎臓学会誌　65　(6-E)　578-578　2023/09

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  13. 形質細胞腫に続発したネフローゼ症候群の一例

    金 沙織, 岡本 好司, 吉田 舞, 長澤 将, 宮崎 真理子, 田中 哲洋

    日本腎臓学会誌　65　(6-E)　587-587　2023/09

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  14. 修正型大血管転移症を伴う三尖弁閉鎖症の末期腎不全患者に対して腹膜透析を導入した一例

    今田 悠介, 岡本 好司, 吉田 舞, 牧野 塁, 長澤 将, 宮崎 真理子, 田中 哲洋

    日本腎臓学会誌　65　(6-E)　606-606　2023/09

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  15. 血液透析導入に至ったアラジール症候群(ALGS)の一例

    吉田 舞, 岡本 好司, 長澤 将, 戸子台 和哲, 宮崎 真理子, 田中 哲洋

    日本透析医学会雑誌　56　(Suppl.1)　779-779　2023/05

    Publisher: (一社)日本透析医学会

    ISSN： 1340-3451

    eISSN： 1883-082X

  16. Marked reduction of proteinuria after removal of a growth hormone-producing pituitary adenoma in a patient with focal segmental glomerulosclerosis: a case report and literature review.

    Shizuka Ishigaki, Yuji Oe, Kei Omata, Yoshikiyo Ono, Yuta Tezuka, Ryo Morimoto, Shun Watanabe, Hiroyasu Nishioka, Fumitoshi Satoh, Mai Yoshida, Rui Makino, Koji Okamoto, Tasuku Nagasawa, Naoko Inoshita, Shozo Yamada, Hiroshi Sato, Sadayoshi Ito, Mariko Miyazaki

    CEN case reports　12　(2)　164-170　2022/10/06

    DOI： 10.1007/s13730-022-00739-6 　

    More details Close

    Focal segmental glomerulosclerosis is a rare complication of acromegaly. A 74-year-old man was found to have acromegaly features such as enlargement of the forehead, nose, and hands. Laboratory tests showed a urine protein/creatinine ratio of 3.16 g/gCr and serum creatinine of 1.34 mg/dL. The levels of growth hormone and insulin-like growth factor I were markedly elevated, and the growth hormone level was not suppressed after 75 g oral glucose loading. Magnetic resonance imaging revealed a pituitary tumor with a diameter of 1.2 cm. Renal biopsy confirmed the diagnosis of focal segmental glomerulosclerosis. Transsphenoidal resection of the pituitary tumor led to remission of acromegaly and reduction in proteinuria highlighting the causal link between growth hormone overproduction and proteinuria. Treatment of acromegaly may be effective for acromegaly-associated focal segmental glomerulosclerosis.

  17. 腎サルコイドーシス患者における新規バイオマーカーの探索

    千葉 祐貴, 牧野 塁, 吉田 舞, 長澤 将, 宮崎 真理子, 田中 哲洋, 村上 康司, 玉田 勉, 岡本 好司

    日本サルコイドーシス/肉芽腫性疾患学会雑誌　42　(サプリメント号)　63-63　2022/10

    Publisher: 日本サルコイドーシス

    ISSN： 1883-1273

    eISSN： 1884-6114

  18. 肺移植後に発症したカルシニューリン阻害薬腎症による末期腎不全に対し腎移植を施行した一例

    今田 悠介, 長澤 将, 吉田 舞, 牧野 塁, 岡本 好司, 宮崎 真理子, 田中 哲洋

    日本腎臓学会誌　64　(6-E)　551-551　2022/10

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  19. 先天性複雑心奇形を伴う慢性腎不全患者に腹膜透析を導入した一例

    玉懸 直人, 岡本 好司, 吉田 舞, 牧野 塁, 長澤 将, 宮崎 真理子, 田中 哲洋

    日本腎臓学会誌　64　(6-E)　555-555　2022/10

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  20. 血液透析を離脱できたCOVID-19治療中に発症した急性尿細管壊死の一例

    古田 銀次, 牧野 塁, 吉田 舞, 岡本 好司, 長澤 将, 宮崎 真理子, 田中 哲洋

    日本腎臓学会誌　64　(6-E)　566-566　2022/10

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  21. Antisense oligonucleotides ameliorate kidney dysfunction in podocyte-specific APOL1 risk variant mice. International-journal

    Ya-Wen Yang, Bibek Poudel, Julia Frederick, Poonam Dhillon, Rojesh Shrestha, Ziyuan Ma, Junnan Wu, Koji Okamoto, Jeffrey B Kopp, Sheri L Booten, Danielle Gattis, Andrew T Watt, Matthew Palmer, Mariam Aghajan, Katalin Susztak

    Molecular therapy : the journal of the American Society of Gene Therapy　30　(7)　2491-2504　2022/07/06

    DOI： 10.1016/j.ymthe.2022.04.007 　

    More details Close

    Coding variants (named G1 and G2) in Apolipoprotein L1 (APOL1) can explain most excess risk of kidney disease observed in African American individuals. It has been proposed that risk variant APOL1 dose, such as increased risk variant APOL1 level serves as a trigger (second hit) for disease development. The goal of this study was to determine whether lowering risk variant APOL1 levels protects from disease development in a podocyte-specific transgenic mouse disease model. We administered antisense oligonucleotides (ASO) targeting APOL1 to podocyte-specific G2APOL1 mice and observed efficient reduction of APOL1 levels. APOL1 ASO1, which more efficiently lowered APOL1 transcript levels, protected mice from albuminuria, glomerulosclerosis, tubulointerstitial fibrosis, and renal failure. Administration of APOL1 ASO1 was effective even for established disease in the NEFTA-rtTA/TRE-G2APOL1 (NEFTA/G2APOL1) mice. We observed a strong correlation between APOL1 transcript level and disease severity. We concluded that APOL1 ASO1 may be an effective therapeutic approach for APOL1-associated glomerular disease.

  22. The physiological and pathophysiological roles of carbohydrate response element binding protein in the kidney.

    Atsushi Yokoyama, Susumu Suzuki, Koji Okamoto, Akira Sugawara

    Endocrine journal　69　(6)　605-612　2022/06/28

    DOI： 10.1507/endocrj.EJ22-0083 　

    More details Close

    Glucose is not only the energy fuel for most cells, but also the signaling molecule which affects gene expression via carbohydrate response element binding protein (ChREBP), a Mondo family transcription factor. In response to high glucose conditions, ChREBP regulates glycolytic and lipogenic genes by binding to carbohydrate response elements (ChoRE) in the regulatory region of its target genes, thus elucidating the role of ChREBP for converting excessively ingested carbohydrates to fatty acids as an energy storage in lipogenic tissues such as the liver and adipose tissue. While the pathophysiological roles of ChREBP for fatty liver and obesity in these tissues are well known, much of the physiological and pathophysiological roles of ChREBP in other tissues such as the kidney remains unclear despite its high levels of expression in them. This review will thus highlight the roles of ChREBP in the kidney and briefly introduce the latest research results that have been reported so far.

  23. 腎サルコイドーシス患者における新規バイオマーカーの探索

    千葉 祐貴, 吉田 舞, 牧野 塁, 長澤 将, 宮崎 真理子, 岡本 好司

    日本腎臓学会誌　64　(3)　291-291　2022/05

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  24. 感染症を繰り返し半月体形成性糸球体腎炎から透析導入に至ったKlippel-Trenauna y-Weber症候群(KTWS)の一例

    吉田 舞, 千葉 祐貴, 高橋 慧, 大江 佑治, 牧野 塁, 岡本 好司, 藤倉 恵美, 長澤 将, 栗原 功, 宮崎 真理子

    日本透析医学会雑誌　55　(Suppl.1)　683-683　2022/05

    Publisher: (一社)日本透析医学会

    ISSN： 1340-3451

    eISSN： 1883-082X

  25. HIV-1 Vpr suppresses expression of the thiazide-sensitive sodium chloride co-transporter in the distal convoluted tubule. International-journal

    Shashi Shrivastav, Hewang Lee, Koji Okamoto, Huiyan Lu, Teruhiko Yoshida, Khun Zaw Latt, Hidefumi Wakashin, James L T Dalgleish, Erik H Koritzinsky, Peng Xu, Laureano D Asico, Joon-Yong Chung, Stephen Hewitt, John J Gildea, Robin A Felder, Pedro A Jose, Avi Z Rosenberg, Mark A Knepper, Tomoshige Kino, Jeffrey B Kopp

    PloS one　17　(9)　e0273313　2022

    DOI： 10.1371/journal.pone.0273313 　

    More details Close

    HIV-associated nephropathy (HIVAN) impairs functions of both glomeruli and tubules. Attention has been previously focused on the HIVAN glomerulopathy. Tubular injury has drawn increased attention because sodium wasting is common in hospitalized HIV/AIDS patients. We used viral protein R (Vpr)-transgenic mice to investigate the mechanisms whereby Vpr contributes to urinary sodium wasting. In phosphoenolpyruvate carboxykinase promoter-driven Vpr-transgenic mice, in situ hybridization showed that Vpr mRNA was expressed in all nephron segments, including the distal convoluted tubule. Vpr-transgenic mice, compared with wild-type littermates, markedly increased urinary sodium excretion, despite similar plasma renin activity and aldosterone levels. Kidneys from Vpr-transgenic mice also markedly reduced protein abundance of the Na+-Cl- cotransporter (NCC), while mineralocorticoid receptor (MR) protein expression level was unchanged. In African green monkey kidney cells, Vpr abrogated the aldosterone-mediated stimulation of MR transcriptional activity. Gene expression of Slc12a3 (NCC) in Vpr-transgenic mice was significantly lower compared with wild-type mice, assessed by both qRT-PCR and RNAScope in situ hybridization analysis. Chromatin immunoprecipitation assays identified multiple MR response elements (MRE), located from 5 kb upstream of the transcription start site and extending to the third exon of the SLC12A3 gene. Mutation of MRE and SP1 sites in the SLC12A3 promoter region abrogated the transcriptional responses to aldosterone and Vpr, indicating that functional MRE and SP1 are required for the SLC12A3 gene suppression in response to Vpr. Thus, Vpr attenuates MR transcriptional activity and inhibits Slc12a3 transcription in the distal convoluted tubule and contributes to salt wasting in Vpr-transgenic mice.

  26. Glomerulonephritis Associated with Infective Endocarditis Showing Serological Positivity for PR3-anti-neutrophil Cytoplasmic Antibody and Anti-glomerular Basement Membrane Antibody.

    Yuki Chiba, Kei Takahashi, Rui Makino, Mai Yoshida, Yuji Oe, Tasuku Nagasawa, Hiroshi Sato, Mariko Miyazaki, Koji Okamoto

    Internal medicine (Tokyo, Japan)　61　(14)　2179-2185　2021/12/28

    DOI： 10.2169/internalmedicine.8385-21 　

    More details Close

    We herein report a case of crescentic glomerulonephritis associated with infective endocarditis (IE). A 61-year-old-woman presented with a fever and renal dysfunction and was diagnosed with IE. The patient was positive for PR3-ANCA and anti-GBM antibodies. Renal biopsy findings showed crescentic glomerulonephritis with isolated deposition of C3c, a serum conversion product of complement C3. Given these clinical findings, the patient was diagnosed with IE-associated glomerulonephritis. Antibiotic therapy was continued without immunosuppressive agents. After the initiation of the antibiotics, the fever resolved, and the renal function gradually recovered. This case highlights the notion that laboratory findings should be carefully evaluated with reference to other findings.

  27. Myeloid cell-derived coagulation tissue factor is associated with renal tubular damage in mice fed an adenine diet

    Shu Yamakage, Yuji Oe, Emiko Sato, Koji Okamoto, Akiyo Sekimoto, Satoshi Kumakura, Hiroshi Sato, Mai Yoshida, Tasuku Nagasawa, Mariko Miyazaki, Sadayoshi Ito, Nigel Mackman, Nobuyuki Takahashi

    Scientific Reports　11　(1)　2021/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-021-91586-5 　

    eISSN： 2045-2322

    More details Close

    <title>Abstract</title>Patients with chronic kidney disease (CKD) commonly exhibit hypercoagulability. Increased levels of uremic toxins cause thrombogenicity by increasing tissue factor (TF) expression and activating the extrinsic coagulation cascade. TF is induced in monocytes and macrophages under pathological conditions, such as inflammatory diseases. However, the role of monocyte myeloid cell TF in CKD progression remains unclear. We aimed to clarify this issue, and the present study found that patients with CKD had elevated levels of D-dimer, a marker of fibrin degradation, which was associated with decreased estimated glomerular filtration rate and increased serum levels of uremic toxins, such as indoxyl sulfate. In vitro studies showed that several uremic toxins increased cellular TF levels in monocytic THP-1 cells. Mice with TF specifically deleted in myeloid cells were fed an adenine diet to cause uremic kidney injury. Myeloid TF deletion reduced tubular injury and pro-inflammatory gene expression in the kidneys of adenine-induced CKD but did not improve renal function as measured by plasma creatinine or blood urea nitrogen. Collectively, our findings suggest a novel concept of pathogenesis of coagulation-mediated kidney injury, in which elevated TF levels in monocytes under uremic conditions is partly involved in the development of CKD.

  28. 組織学的評価を踏まえ腎移植マージナルドナーの適格性を判断した1例

    石岡 広崇, 牧野 塁, 吉田 舞, 大江 佑治, 岡本 好司, 長澤 将, 宮崎 真理子, 戸子台 和哲, 宮城 重人, 佐藤 博

    日本腎臓学会誌　63　(6-E)　724-724　2021/09

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  29. Correction to: Genome-wide association study identifies new loci for albuminuria in the Japanese population.

    Hiroshi Okuda, Koji Okamoto, Michiaki Abe, Kota Ishizawa, Satoshi Makino, Osamu Tanabe, Junichi Sugawara, Atsushi Hozawa, Kozo Tanno, Makoto Sasaki, Gen Tamiya, Masayuki Yamamoto, Sadayoshi Ito, Tadashi Ishii

    Clinical and experimental nephrology　25　(5)　565-565　2021/05

    DOI： 10.1007/s10157-021-02053-4 　

  30. Retrospective study revealed that Zn relate to improvement of swallowing function in the older adults. International-journal

    Yumika Seki, Kota Ishizawa, Tetsuya Akaishi, Michiaki Abe, Koji Okamoto, Junichi Tanaka, Ryutaro Arita, Shin Takayama, Akiko Kikuchi, Mariko Miyazaki, Hideo Harigae, Mayumi Sato, Masaya Hoshi, Kazuaki Hatsugai, Tadashi Ishii

    BMC geriatrics　21　(1)　279-279　2021/04/26

    DOI： 10.1186/s12877-021-02224-8 　

    More details Close

    BACKGROUND: Zinc is an essential micronutrient for maintaining biological activity. The level of zinc in the blood is known to decrease with age, especially in those over 75 years of age. In older adults patients with impaired functional status, aspiration pneumonia based on dysphagia often becomes problematic. However, the relationship between zinc deficiency and swallowing function has not been studied before. METHODS: A total of 52 older adults subjects (15 males and 37 females) living in a nursing home were enrolled for this study. At the time of enrollment, data of gender, age, body weight, serum zinc levels, serum albumin levels, and the time in a simple 2-step swallowing provocation test (S-SPT) were collected. In patients with serum zinc levels < 60 μg/dL, we initiated 2 months of oral zinc supplementation therapy with a 34 mg/day zinc load. Those who underwent zinc supplementation were re-evaluated after the treatment period and serum zinc levels and S-SPT time were measured. RESULTS: At the time of enrollment, serum zinc level was significantly correlated with serum albumin levels (Pearson's R = 0.58, p < 0.0001) and time in the S-SPT (Spearman's rho = - 0.32, p = 0.0219). Twenty-five of the 52 patients had zinc deficiency with a serum zinc level < 60 μg/dL. After 2 months of oral zinc supplementation, both serum zinc levels (p < 0.0001) and time in the S-SPT (p = 0.04) significantly improved. Meanwhile, serum albumin level (p = 0.48) or body weight (p = 0.07) did not significantly change following zinc supplementation. Zinc supplementation significantly improved swallowing function, especially in the older adults who had comorbid dysphagia and zinc deficiency. CONCLUSIONS: Zinc deficiency is associated with compromised swallowing function in older adults patients with impaired general functions. Oral zinc supplementation can alleviate dysphagia in older adults patients with zinc deficiency even though this is a retrospective study. Further study will be needed to confirm this positive effect.

  31. A Novel Mutation in LMX1B (p.Pro219Ala) Causes Focal Segmental Glomerulosclerosis with Alport Syndrome-like Phenotype.

    Yuji Oe, Eikan Mishima, Takayasu Mori, Koji Okamoto, Yohei Honkura, Tasuku Nagasawa, Mai Yoshida, Hiroshi Sato, Jun Suzuki, Ryoukichi Ikeda, Eisei Sohara, Shinichi Uchida, Yukio Katori, Mariko Miyazaki

    Internal medicine (Tokyo, Japan)　60　(18)　2991-2996　2021/04/05

    DOI： 10.2169/internalmedicine.6987-20 　

    More details Close

    A 69-year-old woman presented with mild renal dysfunction, proteinuria, and sensorineural hearing loss. A renal biopsy showed focal segmental glomerulosclerosis with thinning of the glomerular basement membrane. There was a positive family history of end-stage kidney disease and hearing loss. Although Alport syndrome was suspected from these features, a genetic test using next-generation sequencer identified a novel missense mutation in LMX1B, c.655C>G: p. (Pro219Ala). In silico analyses predicted the pathogenicity of the mutation. Thus, the present case was diagnosed as LMX1B-associated nephropathy presenting with Alport syndrome-like phenotype, expanding the disease spectrum of LMX1B nephropathy.

  32. Spontaneous remission of minimal change nephrotic syndrome in an elderly man.

    Yuki Chiba, Tasuku Nagasawa, Saori Kin, Kei Takahashi, Mai Yoshida, Yuji Oe, Koji Okamoto, Hiroshi Sato, Mariko Miyazaki

    CEN case reports　10　(2)　301-307　2021/01/04

    DOI： 10.1007/s13730-020-00554-x 　

    More details Close

    Minimal change nephrotic syndrome (MCNS) cases achieving spontaneous remission without external factors are rarely reported. We report a case of MCNS that achieved spontaneous remission without external factors that triggered its onset. An 82-year-old male patient was admitted to the hospital for close examination of nephrotic syndrome. Renal biopsy was performed and MCNS was diagnosed. Owing to the patient's age and history of foot and microvascular arteriovenous thrombosis, we did not start immunosuppressive drugs, including steroids, and opted for conservative management. After conservative treatment, proteinuria gradually decreased, and the patient achieved complete remission. Given that the patient had a history of urinary protein and thrombosis, recurrence of MCNS was considered again this time. In addition, the involvement of external factors that trigger the onset of MCNS was not found. In conclusion, in elderly-onset MCNS, clinicians generally hesitate to initiate treatment with an immunosuppressive drug, containing steroids, because of its many complications. Thus, our data provide valuable insight into MCNS.

  33. 症例による透析患者の画像診断 維持血液透析患者に発症した進行性多巣性白質脳症の1例 Peer-reviewed

    千葉 祐貴, 割田 仁, 中道 一生, 宮崎 真理子, 小山 千佳, 小島 慶恵, 吉田 舞, 大江 佑治, 岡本 好司, 藤倉 恵美, 長澤 将, 西山 修平

    臨床透析　36　(11)　1473-1476　2020/10

    Publisher: (株)日本メディカルセンター

    ISSN： 0910-5808

    eISSN： 2433-247X

  34. 外的要因無く発症し自然寛解に至った高齢微小変化型ネフローゼ症候群の一例

    千葉 祐貴, 吉田 舞, 大江 佑治, 岡本 好司, 長澤 将, 宮崎 真理子

    日本腎臓学会誌　62　(6)　539-539　2020/09

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  35. 血液透析非導入となった終末期高齢患者の一例 改訂後の「提言」と透析治療の「無益性」

    藤倉 恵美, 金 沙織, 吉田 舞, 大江 祐治, 岡本 好司, 宮崎 真理子

    日本腎臓学会誌　62　(6)　557-557　2020/09

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  36. Questionnaire survey on the prescription of renal replacement therapy for acute phase patients on maintenance dialysis who developed cerebrovascular disease.

    Maho Akiu, Tae Yamamoto, Emi Fujikura, Koji Okamoto, Atsuhiro Nakagawa, Mai Yoshida, Takashi Nakamichi, Taro Fukushi, Tasuku Nagasawa, Yuji Oe, Masaaki Nakayama, Hiroshi Sato, Teiji Tominaga, Sadayoshi Ito, Hideo Harigae, Mariko Miyazaki

    Clinical and experimental nephrology　24　(9)　821-828　2020/09

    DOI： 10.1007/s10157-020-01905-9 　

    More details Close

    BACKGROUND: There is limited information about acute phase renal replacement therapy (RRT) for maintenance hemodialysis patients after the onset of cerebrovascular disease. This study aimed to investigate which modality of renal replacement therapy is currently selected in practice. METHODS: We conducted a mail-based survey in 317 dialysis facilities that were certified by three academic societies that focus on dialysis, neurology, and neurosurgery in Japan. RESULTS: We received responses from 103 facilities (32.5%). In cases of cerebral infarction (CI) and intracerebral hemorrhage (ICH), more than 80% of the facilities selected only intermittent RRT, and 22.3% (CI)/8.7% (ICH) of the facilities selected intermittent HD which is the same setting in normal conditions. Although continuous hemodiafiltration and peritoneal dialysis are recommended in the Japanese guidelines, these were selected in only a few facilities: 16.5% and 0% in CI, 16.5% and 1% in ICH, respectively. RRT on the day of onset tended to be avoided, irrespective of the duration following the last HD session. Furthermore, physicians preferred to modify anticoagulants and reduce dialysis performance in the acute phase. CONCLUSION: This questionnaire survey uncovered a gap between guidelines and actual practice, even in hospitals accredited as educational facility, which is a novel and important finding. Further studies with larger sample sizes are needed to determine the optimal modality of RRT for the acute phase of cerebrovascular disease.

  37. Genome-wide association study identifies new loci for albuminuria in the Japanese population.

    Hiroshi Okuda, Koji Okamoto, Michiaki Abe, Kota Ishizawa, Satoshi Makino, Osamu Tanabe, Junichi Sugawara, Atsushi Hozawa, Kozo Tanno, Makoto Sasaki, Gen Tamiya, Masayuki Yamamoto, Sadayoshi Ito, Tadashi Ishii

    Clinical and experimental nephrology　24　(8)　1-9　2020/08

    DOI： 10.1007/s10157-020-01884-x 　

    More details Close

    BACKGROUND: Urinary albumin excretion (UAE) is a risk factor for cardiovascular diseases, metabolic syndrome, chronic kidney disease, etc. Only a few genome-wide association studies (GWAS) for UAE have been conducted in the European population, but not in the Asian population. Here we conducted GWAS and identified several candidate genes harboring single nucleotide polymorphisms (SNPs) responsible for UAE in the Japanese population. METHODS: We conducted GWAS for UAE in 7805 individuals of Asian ancestry from health-survey data collected by Tohoku Medical Megabank Organization (ToMMo) and Iwate Tohoku Medical Megabank Organization (IMM). The SNP genotype data were obtained with a SNP microarray. After imputation using a haplotype panel consisting of 2000 genome sequencing, 4,962,728 SNP markers were used for the GWAS. RESULTS: Eighteen SNPs at 14 loci (GRM7, EXOC1/NMU, LPA, STEAP1B/RAPGEF5, SEMA3D, PRKAG2, TRIQK, SERTM1, TPT1-AS1, OR5AU1, TSHR, FMN1/RYR3, COPRS, and BRD1) were associated with UAE in the Japanese individuals. A locus with particularly strong associations was observed on TSHR, chromosome 14 [rs116622332 (p = 3.99 × 10-10)]. CONCLUSION: In this study, we successfully identified UAE-associated variant loci in the Japanese population. Further study is required to confirm this association.

  38. 住民コホートを使った尿中アルブミン排泄量に関する量的形質遺伝子座の検討

    岡本 好司, 奥田 拓史, 石井 正, 佐々木 真理, 田宮 元, 山本 雅之, 阿部 倫明

    日本腎臓学会誌　62　(4)　261-261　2020/07

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  39. 住民コホートを使った尿中アルブミン排泄量に関する量的形質遺伝子座の検討

    岡本 好司, 奥田 拓史, 石井 正, 佐々木 真理, 田宮 元, 山本 雅之, 阿部 倫明

    日本腎臓学会誌　62　(4)　261-261　2020/07

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  40. Kidney function, blood pressure and proteinuria were associated with pregnancy outcomes of pregnant women with chronic kidney disease: a single-center, retrospective study in the Asian population.

    Satoshi Kumakura, Koji Okamoto, Saeko Takeuchi, Mai Yoshida, Takashi Nakamichi, Tasuku Nagasawa, Emi Fujikura, Tae Yamamoto, Masatoshi Saito, Takushi Hanita, Michihiro Satoh, Hiroshi Sato, Sadayoshi Ito, Hideo Harigae, Mariko Miyazaki

    Clinical and experimental nephrology　24　(6)　547-556　2020/06

    DOI： 10.1007/s10157-020-01865-0 　

    More details Close

    BACKGROUND: Studies among pregnant Asian women with chronic kidney disease (CKD) have not been widely performed; therefore, clinical criteria for these patients have not been well established. METHODS: We conducted a retrospective study among pregnant women with CKD who received prenatal care at our institution for 8 consecutive years. Primary outcome was the development of severe adverse events (SAEs). We analyzed correlations between primary outcome and CKD parameters [age, body mass index (BMI), estimated glomerular filtration rate (eGFR), urinary protein-creatinine ratio (UP), systolic blood pressure (SBP), diastolic blood pressure (DBP), and not normal blood pressure (non-NBP)] at the time of referral. Secondary outcomes were low birth weight (LBW), preterm delivery (PreD), and small for gestational age (SGA). We divided into two categories, CKD stage G1, and G2 or higher according to eGFR, and proteinuria negative and proteinuria positive according to UP, respectively. RESULTS: We observed 89 pregnancies. SAE was observed in 28 pregnancies. In live birth cases, there were 28 PreD, 28 LBW and 13 SGA. Major SAEs included preeclampsia, superimposed preeclampsia, unscheduled cesarean section, neonatal intensive care unit admission, and fetal death. Stepwise logistic regression analysis selected eGFR (OR = 0.847, p = 0.026), SBP (OR = 1.897, p = 0.006) and proteinuria positive (OR = 2.96, p = 0.046) as the significant predictors of SAEs. There were no significant differences among the baseline characteristics stratified by SGA. CONCLUSIONS: This is the first study to report pregnancy outcomes among Japanese non-disease-oriented patients with CKD. In Asians, especially in the Japanese population, kidney function, blood pressure and proteinuria might affect pregnancy outcomes.

  41. IgA腎症患者は妊娠前に治療し血圧正常化を目指すべき IgA腎症合併妊娠の産科合併症リスクの検討

    熊倉 慧, 竹内 沙永子, 吉田 舞, 岡本 好司, 中道 崇, 長澤 将, 山本 多恵, 藤倉 恵美, 宮崎 真理子

    日本腎臓学会誌　61　(3)　305-305　2019/05

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  42. APOL1 renal risk variants promote cholesterol accumulation in tissues and cultured macrophages from APOL1 transgenic mice. International-journal

    Jung-Hwa Ryu, Mengyuan Ge, Sandra Merscher, Avi Z Rosenberg, Marco Desante, Hila Roshanravan, Koji Okamoto, Myung K Shin, Maarten Hoek, Alessia Fornoni, Jeffrey B Kopp

    PloS one　14　(4)　e0211559　2019

    DOI： 10.1371/journal.pone.0211559 　

    More details Close

    Apolipoprotein L1 (APOL1) genetic variants G1 and G2, compared to the common allele G0, are major risk factors for non-diabetic kidney disease in African descent populations. APOL1 is a minor protein component of HDL, as well as being expressed in podocytes and vascular cells. Reverse cholesterol transport involves the transport of cholesterol to HDL by cellular ATP-binding cassette; ABCA1 and ABCG1 with subsequent delivery from peripheral tissues to the liver. With impaired reverse cholesterol transport, lipid accumulation occurs and macrophages morphologically transform into foam cells, releasing inflammatory factors. We asked whether the APOL1 risk variants alter peripheral cholesterol metabolism and specifically affect macrophage cholesterol efflux. Tissues and bone marrow (BM)-derived monocytes were isolated from wild-type mice (WT) and from BAC/APOL1 transgenic (APOL1-G0, APOL1-G1, and APOL1-G2) mice, which carry a bacterial artificial chromosome that contains the human APOL1 genomic region. Monocytes were differentiated into macrophages using M-CSF, and then polarized into M1 and M2 macrophages. Cholesterol content, cholesterol efflux, and ABCA1 and ABCG1 mRNA expression were measured. Kidney, spleen, and bone marrow-derived macrophages from APOL1-G1 and -G2 mice showed increased cholesterol accumulation and decreased ABCA1 and ABCG1 mRNA levels. BM-derived macrophages from APOL1-G1 and -G2 mice showed significantly reduced cholesterol efflux compared to WT or APOL1-G0 macrophages. Taken together, the evidence suggests that APOL1-G1 and -G2 risk variants impaired reverse cholesterol transport through decreased expression of cholesterol efflux transporters suggesting a possible mechanism to promote macrophage foam cell formation, driving inflammation in the glomerulus and renal interstitium.

  43. ApoL1 renal risk variants induce aberrant THP-1 monocyte differentiation and increase eicosanoid production via enhanced expression of cyclooxygenase-2. International-journal Peer-reviewed

    Hewang Lee, Hila Roshanravan, Ying Wang, Koji Okamoto, Junghwa Ryu, Shashi Shrivastav, Peng Qu, Jeffrey B Kopp

    American journal of physiology. Renal physiology　315　(1)　F140-F150-F150　2018/07/01

    DOI： 10.1152/ajprenal.00254.2017 　

    ISSN： 1931-857X

    More details Close

    Apolipoprotein L1 ( ApoL1) genetic variants are strongly associated with kidney diseases. We investigated the role of ApoL1 variants in monocyte differentiation and eicosanoid production in macrophages, as activated tissue macrophages in kidney might contribute to kidney injury. In human monocyte THP-1 cells, transient overexpression of ApoL1 (G0, G1, G2) by transfection resulted in a 5- to 11-fold increase in CD14 and CD68 gene expression, similar to that seen with phorbol-12-myristate acetate treatment. All ApoL1 variants caused monocytes to differentiate into atypical M1 macrophages with marked increase in M1 markers CD80, TNF, IL1B, and IL6 and modest increase in the M2 marker CD163 compared with control cells. ApoL1-G1 transfection induced additional CD206 and TGFB1 expression, and ApoL1-G2 transfection induced additional CD204 and TGFB1 expression. Gene expression of prostaglandin E2 (PGE2) synthase and thromboxane synthase and both gene and protein expression of cyclooxygenase-2 (COX-2) were increased by ApoL1-G1 and -G2 variants compared with -G0 transfection. Higher levels of PGE2 and thromboxane B2, a stable metabolite of thromboxane A2, and transforming growth factor (TGF)-β1 were released into the supernatant of cultured THP-1 cells transfected with ApoL1-G1 and -G2, but not -G0. The increase in PGE2, thromboxane B2, and TGF-β1 was inhibited by COX-2-specific inhibitor CAY10404 but not by COX-1-specific inhibitor SC-560. These results demonstrate a novel role of ApoL1 variants in the regulation of monocyte differentiation and eicosanoid metabolism, which could modify the immune response and promote inflammatory signaling within the local targeted organs and tissues including the kidney.

  44. Hierarchical Assembly of siRNA with Tetraamino Fullerene in Physiological Conditions for Efficient Internalization into Cells and Knockdown. International-journal Peer-reviewed

    Kosuke Minami, Koji Okamoto, Koji Harano, Eisei Noiri, Eiichi Nakamura

    ACS applied materials & interfaces　10　(23)　19347-19354　2018/06/13

    Publisher: American Chemical Society

    DOI： 10.1021/acsami.8b01869 　

    ISSN： 1944-8252 1944-8244

  45. Apolipoprotein L1 nephropathies: 2017 in review. International-journal Peer-reviewed

    Jeffrey B Kopp, Hila Roshanravan, Koji Okamoto

    Current opinion in nephrology and hypertension　27　(3)　153-158　2018/05

    DOI： 10.1097/MNH.0000000000000399 　

    ISSN： 1062-4821

    More details Close

    PURPOSE OF REVIEW: To review publications relating to apolipoprotein L1 (APOL1) renal risk variants published 2017. RECENT FINDINGS: The study of APOL1 variants continues to be highly active; 24 articles published in 2017 were selected to highlight. These include clinical studies of kidney disease, kidney transplantation, hypertension, cardiovascular disease, and genetic diversity. Laboratory studies included APOL1 association with vesicle-associated membrane soluble N-ethylmaleimide-sensitive factor activating protein receptor protein and with soluble urokinase-type plasminogen activator receptor, mitochondrial dysfunction, endolysosomal dysfunction, and inflammasome activation. SUMMARY: Our understanding of the role of APOL1 genetic variants and the mechanisms for renal toxicity continues to deepen. It is not yet clear which pathways are most relevant to human disease, and so, the most relevant drug targets remain to be defined.

  46. APOL1 risk allele RNA contributes to renal toxicity by activating protein kinase R. International-journal Peer-reviewed

    Koji Okamoto, Jason W Rausch, Hidefumi Wakashin, Yulong Fu, Joon-Yong Chung, Patrick D Dummer, Myung K Shin, Preeti Chandra, Kosuke Suzuki, Shashi Shrivastav, Avi Z Rosenberg, Stephen M Hewitt, Patricio E Ray, Eisei Noiri, Stuart F J Le Grice, Maarten Hoek, Zhe Han, Cheryl A Winkler, Jeffrey B Kopp

    Communications biology　1　188-188　2018

    DOI： 10.1038/s42003-018-0188-2 　

    More details Close

    APOL1 risk alleles associate with chronic kidney disease in African Americans, but the mechanisms remain to be fully understood. We show that APOL1 risk alleles activate protein kinase R (PKR) in cultured cells and transgenic mice. This effect is preserved when a premature stop codon is introduced to APOL1 risk alleles, suggesting that APOL1 RNA but not protein is required for the effect. Podocyte expression of APOL1 risk allele RNA, but not protein, in transgenic mice induces glomerular injury and proteinuria. Structural analysis of the APOL1 RNA shows that the risk variants possess secondary structure serving as a scaffold for tandem PKR binding and activation. These findings provide a mechanism by which APOL1 variants damage podocytes and suggest novel therapeutic strategies.

  47. Glypican-5 Increases Susceptibility to Nephrotic Damage in Diabetic Kidney Peer-reviewed

    Koji Okamoto, Kenjiro Honda, Kent Doi, Tomoko Ishizu, Daisuke Katagiri, Takehiko Wada, Kenji Tomita, Takayasu Ohtake, Toyoji Kaneko, Shuzo Kobayashi, Masaomi Nangaku, Katsushi Tokunaga, Eisei Noiri

    AMERICAN JOURNAL OF PATHOLOGY　185　(7)　1889-1898　2015/07

    DOI： 10.1016/j.ajpath.2015.03.025 　

    ISSN： 0002-9440

    eISSN： 1525-2191

  48. シスプラチン急性腎障害における腸管由来GLP-1の効果

    片桐 大輔, 濱崎 敬文, 土井 研人, 岡本 好司, 根岸 康介, 南学 正臣, 野入 英世

    日本腎臓学会誌　56　(3)　322-322　2014/05

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  49. siRNA delivery targeting to the lung via agglutination-induced accumulation and clearance of cationic tetraamino fullerene Peer-reviewed

    Kosuke Minami, Koji Okamoto, Kent Doi, Koji Harano, Eisei Noiri, Eiichi Nakamura

    SCIENTIFIC REPORTS　4　4916　2014/05

    DOI： 10.1038/srep04916 　

    ISSN： 2045-2322

  50. Protection of Glucagon-Like Peptide-1 in Cisplatin-Induced Renal Injury Elucidates Gut-Kidney Connection Peer-reviewed

    Daisuke Katagiri, Yoshifumi Hamasaki, Kent Doi, Koji Okamoto, Kousuke Negishi, Masaomi Nangaku, Eisei Noiri

    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY　24　(12)　2034-2043　2013/12

    DOI： 10.1681/ASN.2013020134 　

    ISSN： 1046-6673

    eISSN： 1533-3450

  51. HIV-1 Vpr Enhances PPAR beta/delta-Mediated Transcription, Increases PDK4 Expression, and Reduces PDC Activity Peer-reviewed

    Shashi Shrivastav, Liyan Zhang, Koji Okamoto, Hewang Lee, Claudia Lagranha, Yoshifusa Abe, Ashok Balasubramanyam, Gary D. Lopaschuk, Tomoshige Kino, Jeffrey B. Kopp

    MOLECULAR ENDOCRINOLOGY　27　(9)　1564-1576　2013/09

    DOI： 10.1210/me.2012-1370 　

    ISSN： 0888-8809

  52. Specific antibody in IV immunoglobulin for postsplenectomy sepsis Peer-reviewed

    Kensuke Nakamura, Kent Doi, Koji Okamoto, Syunnya Arai, Satoshi Ueha, Kouji Matsushima, Susumu Nakajima, Naoki Yahagi, Eisei Noiri

    Critical Care Medicine　41　(8)　e163-e170　2013/08

    DOI： 10.1097/CCM.0b013e318287f1de 　

    ISSN： 0090-3493 1530-0293

  53. Protein-coated nanocapsules via multilevel surface modification. Controlled preparation and microscopic analysis at nanometer resolution Peer-reviewed

    Koji Harano, Kosuke Minami, Eisei Noiri, Koji Okamoto, Eiichi Nakamura

    CHEMICAL COMMUNICATIONS　49　(34)　3525-3527　2013

    DOI： 10.1039/c3cc40752g 　

    ISSN： 1359-7345

  54. 3-Hydroxy-3-methylglutaryl-coenzyme A reductase inhibitor simvastatin ameliorates renal fibrosis through HOXA13-USAG-1 pathway Peer-reviewed

    Yoshifumi Hamasaki, Kent Doi, Koji Okamoto, Hideaki Ijichi, George Seki, Rui Maeda-Mamiya, Toshiro Fujita, Eisei Noiri

    LABORATORY INVESTIGATION　92　(8)　1161-1170　2012/08

    DOI： 10.1038/labinvest.2012.71 　

    ISSN： 0023-6837

  55. Inhibition of Glucose-Stimulated Insulin Secretion by KCNJ15, a Newly Identified Susceptibility Gene for Type 2 Diabetes Peer-reviewed

    Koji Okamoto, Naoko Iwasaki, Kent Doi, Eisei Noiri, Yasuhiko Iwamoto, Yasuko Uchigata, Toshiro Fujita, Katsushi Tokunaga

    DIABETES　61　(7)　1734-1741　2012/07

    DOI： 10.2337/db11-1201 　

    ISSN： 0012-1797

  56. A novel mechanism in maggot debridement therapy: protease in excretion/secretion promotes hepatocyte growth factor production Peer-reviewed

    Kenjiro Honda, Koji Okamoto, Yasuhiro Mochida, Kunihiro Ishioka, Machiko Oka, Kyoko Maesato, Ryota Ikee, Hidekazu Moriya, Sumi Hidaka, Takayasu Ohtake, Kent Doi, Toshiro Fujita, Shuzo Kobayashi, Eisei Noiri

    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY　301　(6)　C1423-C1430　2011/12

    DOI： 10.1152/ajpcell.00065.2011 　

    ISSN： 0363-6143

  57. Common variation in GPC5 is associated with acquired nephrotic syndrome Peer-reviewed

    Koji Okamoto, Katsushi Tokunaga, Kent Doi, Toshiro Fujita, Hodaka Suzuki, Tetsuo Katoh, Tsuyoshi Watanabe, Nao Nishida, Akihiko Mabuchi, Atsushi Takahashi, Michiaki Kubo, Shiro Maeda, Yusuke Nakamura, Eisei Noiri

    NATURE GENETICS　43　(5)　459-+　2011/05

    DOI： 10.1038/ng.792 　

    ISSN： 1061-4036

  58. Serum biological antioxidant potential predicts the prognosis of hemodialysis patients. International-journal

    Tomoko Ishii, Takayasu Ohtake, Koji Okamoto, Yasuhiro Mochida, Kunihiro Ishioka, Machiko Oka, Kyoko Maesato, Ryota Ikee, Hidekazu Moriya, Sumi Hidaka, Kent Doi, Eisei Noiri, Toshiro Fujita, Shuzo Kobayashi

    Nephron. Clinical practice　117　(3)　c230-6　2011

    DOI： 10.1159/000320201 　

    More details Close

    BACKGROUND: It is well known that oxidative stress is enhanced in patients with end-stage renal disease. However, little is known about the relationship between serum antioxidant capacity and clinical outcome in hemodialysis (HD) patients. METHODS: We examined the relationship between serum biomarkers of oxidative stress and clinical outcomes including all-cause mortality, hospitalization rate and incidence of cardiovascular events in HD patients. As biomarkers of oxidative stress, we measured serum levels of coenzyme Q10 (CoQ10) and biological antioxidant potential (BAP). RESULTS: 108 patients were observed for 30 months as the follow-up periods. The survival group (n = 83) showed significantly higher BAP values compared with those in death groups (n = 25; p < 0.05). When serum BAP levels were divided into two groups by their median value, the group with higher BAP values had a better survival rate than that with lower BAP values on the Kaplan-Meier survival analysis (p = 0.05). Although serum levels of CoQ10 did not show any association with clinical outcomes, lower BAP was selected as an independent risk factor for all-cause mortality as well as the absence of angiotensin-converting enzyme inhibitors/angiotensin II receptor blockers therapy by age-adjusted Cox regression analysis. CONCLUSIONS: This study indicated that BAP could predict the prognosis of HD patients.

  59. Acute tubular necrosis due to cutaneous contact with cresol Peer-reviewed

    Koji Okamoto, Eisei Noiri, Machiko Oka, Hidekazu Moriya, Takayasu Ohtake, Shuzo Kobayashi

    BMJ Case Reports　2011　2011

    Publisher: BMJ Publishing Group

    DOI： 10.1136/bcr.08.2010.3213 　

    ISSN： 1757-790X

  60. Neutrophil Elastase Contributes to Acute Lung Injury Induced by Bilateral Nephrectomy Peer-reviewed

    Tomoko Ishii, Kent Doi, Koji Okamoto, Mitsuru Imamura, Makoto Dohi, Kazuhiko Yamamoto, Toshiro Fujita, Eisei Noiri

    AMERICAN JOURNAL OF PATHOLOGY　177　(4)　1665-1673　2010/10

    DOI： 10.2353/ajpath.2010.090793 　

    ISSN： 0002-9440

  61. In vivo gene delivery by cationic tetraamino fullerene Peer-reviewed

    Rui Maeda-Mamiya, Eisei Noiri, Hiroyuki Isobe, Waka Nakanishi, Koji Okamoto, Kent Doi, Takeshi Sugaya, Tetsuro Izumi, Tatsuya Homma, Eiichi Nakamura

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　107　(12)　5339-5344　2010/03

    DOI： 10.1073/pnas.0909223107 　

    ISSN： 0027-8424

  62. Identification of KCNJ15 as a Susceptibility Gene in Asian Patients with Type 2 Diabetes Mellitus Peer-reviewed

    Koji Okamoto, Naoko Iwasaki, Chisa Nishimura, Kent Doi, Eisei Noiri, Shinko Nakamura, Miho Takizawa, Makiko Ogata, Risa Fujimaki, Niels Grarup, Charlotta Pisinger, Knut Borch-Johnsen, Torsten Lauritzen, Annelli Sandbaek, Torben Hansen, Kazuki Yasuda, Haruhiko Osawa, Kishio Nanjo, Takashi Kadowaki, Masato Kasuga, Oluf Pedersen, Toshiro Fujita, Naoyuki Kamatani, Yasuhiko Iwamoto, Katsushi Tokunaga

    AMERICAN JOURNAL OF HUMAN GENETICS　86　(1)　54-64　2010/01

    DOI： 10.1016/j.ajhg.2009.12.009 　

    ISSN： 0002-9297

  63. No evidence for association between the interferon regulatory factor 1 (IRF1) gene and clinical tuberculosis Peer-reviewed

    Sabine Vollstedt, Rika Yuliwulandari, Koji Okamoto, Luu Thi Lien, Naoto Keicho, Jecti Teguh Rochani, Riyani Wikaningrum, Katsushi Tokunaga

    TUBERCULOSIS　89　(1)　71-76　2009/01

    DOI： 10.1016/j.tube.2008.09.006 　

    ISSN： 1472-9792

  64. Genome study of kidney disease in the age of post genome-sequencing Peer-reviewed

    Kent Doi, Koji Okamoto, Katsushi Tokunaga, Toshiro Fujita, Eisei Noiri

    Endocrine, Metabolic and Immune Disorders - Drug Targets　8　(3)　173-183　2008/09

    DOI： 10.2174/187153008785700136 　

    ISSN： 1871-5303

  65. 透析患者における下肢閉塞性動脈硬化症 無侵襲診断法について

    岡本 好司, 岡 真知子, 真栄里 恭子, 真野 勉, 池江 亮太, 守矢 秀和, 大竹 剛靖, 小林 修三

    脈管学　46　(6)　829-835　2006/12

    Publisher: (一社)日本脈管学会

    ISSN： 0387-1126

  66. Peripheral arterial occlusive disease is more prevalent in patients with hemodialysis: Comparison with the findings of multidetector-row computed tomography Peer-reviewed

    Koji Okamoto, Machiko Oka, Kyoko Maesato, Ryota Ikee, Tsutomu Mano, Hidekazu Moriya, Takayasu Ohtake, Shuzo Kobayashi

    AMERICAN JOURNAL OF KIDNEY DISEASES　48　(2)　269-276　2006/08

    DOI： 10.1053/j.ajkd.200604.075 　

    ISSN： 0272-6386

  67. F-18-fluorodeoxyglucose positron emission tomography in a case retroperitoneal fibrosis Peer-reviewed

    Aiko Sakamoto, Koji Okamoto, Nobukazu Ishizaka, Kazuaki Tejima, Yasunobu Hirata, Ryozo Nagai

    INTERNATIONAL HEART JOURNAL　47　(4)　645-650　2006/07

    DOI： 10.1536/ihj.47.645 　

    ISSN： 1349-2365

  68. Ticlopidine-induced lupus with renal involvement Peer-reviewed

    Takayasu Ohtake, Shuzo Kobayashi, Koji Okamoto, Machiko Oka, Kyoko Maesato, Takeo Yasu, Hidekazu Moriya

    NEPHROLOGY DIALYSIS TRANSPLANTATION　21　(7)　1992-1993　2006/07

    DOI： 10.1093/ndt/gfl028 　

    ISSN： 0931-0509

  69. LDL-apheresis reduces P-selectin, CRP and fibrinogen - Possible important implications for improving atherosclerosis Peer-reviewed

    S Kobayashi, M Oka, H Moriya, K Maesato, K Okamoto, T Ohtake

    THERAPEUTIC APHERESIS AND DIALYSIS　10　(3)　219-223　2006/06

    DOI： 10.1111/j.1744-9987.2006.00332.x 　

    ISSN： 1744-9979

  70. Attenuation of folic acid-induced renal inflammatory injury in platelet-activating factor receptor-deficient mice Peer-reviewed

    K Doi, K Okamoto, K Negishi, Y Suzuki, A Nakao, T Fujita, A Toda, T Yokomizo, Y Kita, Y Kihara, S Ishii, T Shimizu, E Noiri

    AMERICAN JOURNAL OF PATHOLOGY　168　(5)　1413-1424　2006/05

    DOI： 10.2353/ajpath.2006.050634 　

    ISSN： 0002-9440

  71. Efficacy of darbepoetin in doxorubicin-induced cardiorenal injury in rats. International-journal Peer-reviewed

    Noiri E, Nagano N, Negishi K, Doi K, Miyata S, Abe M, Tanaka T, Okamoto K, Hanafusa N, Kondo Y, Ishizaka N, Fujita T

    Nephron. Experimental nephrology　104　(1)　e6-e14　2006

    DOI： 10.1159/000093258 　

    ISSN： 1660-8151

    eISSN： 1660-2129

    More details Close

    This study was intended to elucidate the efficacy of an erythropoietin analog in cardiorenal dysfunction syndrome using a rodent model. Cardiorenal dysfunction was induced using doxorubicin hydrochloride (DXR). Lower doses (3 microg/kg) and higher doses (30 microg/kg) of darbepoetin alfa (DA) were used for intervention. Blood examinations for creatinine, blood urea nitrogen, iron, and hemoglobin were performed until 11 weeks after starting DA administration. Urine collection was performed 10 weeks after starting DA, and protein, iron, and N-acetyl-beta-D-glucosaminidase levels and antioxidation capacity of DA were determined. The dry left ventricular heart weight was measured, when the animals were sacrificed 11 weeks after starting DA administration. Histological analyses were performed for interstitial fibrotic changes and iron deposition in the kidney. Administration of DA markedly improved anemia to the normal control level and significantly alleviated DXR-induced increases of creatinine, blood urea nitrogen, renal interstitial fibrosis, renal iron deposition, and dry left ventricular weight, but serum and urinary iron and urinary protein and N-acetyl-beta-D-glucosaminidase levels were unchanged. The urinary total radical-trapping antioxidant capacity was improved to the normal control level in DA-treated animals. DA reduced the DXR-induced cardiorenal injury. This improvement was achieved, when anemia was corrected to the normal control level.

  72. PMX-DHP エンドトキシン吸着療法(PMX)の効果判定,予後規定因子に関する考察

    岡本 好司, 真栄里 恭子, 守矢 英和, 大竹 剛靖, 小林 修三

    ICUとCCU　28　(別冊)　S200-S202　2004/02

    Publisher: 医学図書出版(株)

    ISSN： 0389-1194

Show all ︎Show first 5

Misc. 16

  1. 医療倫理コンサルテーションチームによる腎代替療法の継続/中止の検討

    藤倉 恵美, 小島 慶恵, 吉田 舞, 小山 千佳, 千葉 祐貴, 岡本 好司, 長澤 将, 宮崎 真理子

    日本透析医学会雑誌　53　(Suppl.1)　370-370　2020/10

    Publisher: (一社)日本透析医学会

    ISSN： 1340-3451

    eISSN： 1883-082X

  2. 血胸を契機に発見された縦隔内異所性副甲状腺腺腫の一例

    千葉 祐貴, 小島 慶恵, 吉田 舞, 大江 佑治, 岡本 好司, 藤倉 恵美, 長澤 将, 宮崎 真理子, 岡田 克典

    日本透析医学会雑誌　53　(Suppl.1)　555-555　2020/10

    Publisher: (一社)日本透析医学会

    ISSN： 1340-3451

    eISSN： 1883-082X

  3. 適切な分娩計画により、安全に出産をした長期血液透析患者の一例

    吉田 舞, 藤倉 恵美, 竹内 沙永子, 岡本 好司, 長澤 将, 中道 崇, 加藤 政子, 植田 卓志, 齋藤 昌利, 宮崎 真理子, 伊藤 貞嘉

    日本透析医学会雑誌　52　(Suppl.1)　787-787　2019/05

    Publisher: (一社)日本透析医学会

    ISSN： 1340-3451

    eISSN： 1883-082X

  4. Genetic Variants in the Lanosterol Synthase Gene Are Associated with Type 2 Diabetes

    Naoko Iwasaki, Koji Okamoto, Kenjiro Honda, Akira Saito, Miho Takizawa, Makiko Ogata, Kazuki Yasuda, Torben Hansen, Oluf Pedersen, Kyong Soo Park, Weiping Jia, Cheng Hu, Yasuko Uchigata, Katsushi Tokunaga

    DIABETES　64　A460-A460　2015/06

    ISSN： 0012-1797

    eISSN： 1939-327X

  5. LSS多型は、内臓肥満やインスリン抵抗性を介して糖尿病発症に関連する

    岡本 好司, 山下 徹志, 本田 謙次郎, 蔵野 信, 塚本 和久, 野入 英世, 南学 正臣, 門脇 孝, 岩崎 直子, 徳永 勝士

    肥満研究　19　(Suppl.)　159-159　2013/09

    Publisher: (一社)日本肥満学会

    ISSN： 1343-229X

  6. 水溶性フラーレン誘導体を用いた肺選択的siRNAデリバリー

    南皓輔, 原野幸治, 岡本好司, 野入英世, 土井研人, 中村栄一

    日本化学会講演予稿集　93rd　(3)　2013

    ISSN： 0285-7626

  7. 遺伝・ゲノム学 腎領域におけるゲノムワイド関連解析

    岡本 好司, 野入 英世

    医学のあゆみ　240　(12)　983-984　2012/03

    Publisher: 医歯薬出版(株)

    ISSN： 0039-2359

  8. カチオン性アミノフラーレンを用いたsiRNAデリバリー

    南皓輔, 岡本好司, 野入英世, 原野幸治, 中村栄一

    日本DDS学会学術集会プログラム予稿集　28th　2012

  9. 腎臓内科学 ネフローゼ症候群とGlypican-5多型の関連

    岡本 好司, 野入 英世

    医学のあゆみ　240　(2)　181-182　2012/01

    Publisher: 医歯薬出版(株)

    ISSN： 0039-2359

  10. Common variation in lanosterol synthase gene predispose to type 2 diabetes

    N. Iwasaki, K. Okamoto, E. Noiri, M. Takizawa, M. Ogata, T. Hansen, O. Pedersen, K. Park, Y. Iwamoto, K. Tokunaga

    DIABETOLOGIA　54　S125-S126　2011/09

    ISSN： 0012-186X

  11. 糖尿病・内分泌代謝学 アジア集団における新規2型糖尿病感受性遺伝子KCNJ15

    岡本 好司

    医学のあゆみ　234　(11)　1058-1059　2010/09

    Publisher: 医歯薬出版(株)

    ISSN： 0039-2359

  12. Effect of Promoter Region Polymorphisms in N-Acetyltransferase 2 (NAT2) Phenotype

    Rika Yuliwulandari, Koji Okamoto, Dharma Permana, Katsushi Tokunaga

    DRUG METABOLISM REVIEWS　42　156-157　2010/08

    ISSN： 0360-2532

  13. Simvastatin ameliorates renal fibrosis via suppressing uterine sensitization-associated gene-1 (USAG-1), a BMP-7 antagonist, in mouse adenine-induced tubulointerstitial injury model

    Yoshifumi Hamasaki, Koji Okamoto, Kent Doi, Toshiro Fujita, Eisei Noiri

    FASEB JOURNAL　24　2010/04

    ISSN： 0892-6638

  14. Association study based on the genome wide linkage results revealed two genes on chromosome 21 in type 2 diabetic subjects in Japanese

    Naoko Iwasaki, Koji Okamoto, Shinko Nakamura, Makiko Ogata, Katsushi Tokunaga, Naoyuki Kamatani, Yasuhiko Iwamoto

    DIABETES　56　A645-A645　2007/06

    ISSN： 0012-1797

  15. アドリアマイシンによる心腎症候群モデルに対するDarbepoetinの効果

    野入 英世, 永野 伸郎, 根岸 康介, 土井 研人, 宮田 そのえ, 阿部 めぐみ, 田中 珠美, 岡本 好司, 花房 規男, 近藤 靖司, 石坂 信和, 藤田 敏郎

    日本腎臓学会誌　48　(3)　283-283　2006/04

    Publisher: (一社)日本腎臓学会

    ISSN： 0385-2385

    eISSN： 1884-0728

  16. CLINICOPATHOLOGICAL STUDY OF RENAL DISEASES WITH MILD PROTEINURIA

    Koji Okamoto, Machiko Oka, Kyoko Maesato, Hidekazu Moriya, Takayasu Ohtake, Shuzo Kobayashi

    NEPHROLOGY　10　A159-A160　2005/06

    ISSN： 1320-5358

Show all ︎Show first 5

Books and Other Publications 5

  1. CRRT療法ポケットマニュアル第2版

    岡本 好司

    医歯薬出版　2015

  2. The Concise Manual of Apheresis Therapy

    Koji Okamoto

    Springer　2014

  3. CRRT療法ポケットマニュアル

    岡本 好司

    医歯薬出版　2011

  4. アフェレーシス療法ポケットマニュアル第2版

    岡本 好司

    医歯薬出版　2011

  5. アフェレーシス療法ポケットマニュアル

    岡本 好司

    医歯薬出版　2010

Presentations 45

  1. Maggot debridement therapyの組織治癒機構

    鈴木 みなみ, 本田 謙次郎, 岡本 好司, 土井 研人, 守矢 英和, 大竹 剛靖, 日高 寿美, 野入 英世, 小林 修三, 南学 正臣

    日本下肢救済・足病学会誌　2015/07

  2. 2型糖尿病との関連が見いだされたlanosterol synthase(LSS)遺伝子多型の他民族における再現性の検討

    岩崎 直子, 岡本 好司, 野入 永生, 滝澤 美保, 尾形 真規子, 富岡 光枝, Hansen Torben, Pedersen Oluf, Park Kyong Soo, Hu Cheng, 徳永 勝士, 内潟 安子

    糖尿病　2015/04

  3. LSS多型は、内臓肥満やインスリン抵抗性を介して糖尿病発症に関連する

    岡本 好司, 山下 徹志, 本田 謙次郎, 蔵野 信, 塚本 和久, 野入 英世, 南学 正臣, 門脇 孝, 岩崎 直子, 徳永 勝士

    肥満研究　2013/09

  4. マゴットセラピーはExcretion/Secretionに含まれるproteaseを介してHepatocyte Growth Factorを産生させる

    本田 謙次郎, 岡本 好司, 持田 泰寛, 石岡 邦啓, 岡 真知子, 真栄里 恭子, 守矢 英和, 日高 寿美, 大竹 剛靖, 土井 研人, 野入 英世, 小林 修三

    脈管学　2011/09

  5. マウス両腎摘-急性腎障害モデルは急性肺障害を惹起し、好中球エラスターゼがその病態に関与する

    石井 智子, 土井 研人, 岡本 好司, 根岸 康介, 藤田 敏郎, 野入 英世

    日本急性血液浄化学会雑誌　2010/10

  6. 透析患者における末梢動脈硬化症と予後の検討

    岡本 好司, 野入 英世, 藤田 敏郎, 本田 謙次郎, 石岡 邦啓, 岡 真知子, 池江 亮太, 日高 寿美, 大竹 剛靖, 小林 修三

    日本透析医学会雑誌　2009/05

  7. 好中球エラスターゼ阻害剤(エラスポール)はマウス両腎摘モデルによる急性肺障害を改善する

    石井 智子, 野入 英世, 土井 研人, 岡本 好司, 根岸 康介, 藤田 敏郎

    日本腎臓学会誌　2009/04

  8. 高ミオグロビン血症に対するβ2ミクログロブリン吸着カラムの有効性の検討

    岡本 好司, 浜崎 敬文, 野入 英世, 藤田 敏郎, 本田 謙次郎, 岡 真知子, 池江 亮太, 守矢 英和, 日高 寿美, 大竹 剛靖, 小林 修三

    日本腎臓学会誌　2009/04

  9. 2型糖尿病関連遺伝子はどこまで解明されたか 全ゲノム連鎖解析結果に基づいた新規2型糖尿病感受性遺伝子の同定

    岩崎 直子, 岡本 好司, 野入 英世, 尾形 真規子, 鎌谷 直之, 徳永 勝士, 岩本 安彦

    糖尿病　2009/04

  10. 血液透析患者における心理社会的背景と治療コンプライアンスについて

    浅野 雅子, 岡本 好司, 本田 謙二郎, 岡 真知子, 池江 亮太, 大竹 剛靖, 小林 修三, 秋田 恭子

    日本透析医学会雑誌　2008/05

  11. 透析患者の心血管障害のガイドラインを考える 各専門分野からの提言 末梢動脈疾患

    小林 修三, 岡本 好司, 守矢 英和, 大竹 剛靖

    日本透析医学会雑誌　2008/05

  12. 血液透析患者における心理社会的背景とセルフケアについて

    浅野 雅子, 岡本 好司, 本田 謙二郎, 岡 真知子, 真野 勉, 池江 亮太, 大竹 剛靖, 小林 修三, 秋田 恭子

    日本透析医学会雑誌　2008/05

  13. Acute Kidney Injuryにおける尿中Biomarkerとしての肝臓型脂肪酸結合蛋白(L-FABP)の有用性

    根岸 康介, 野入 英世, 菅谷 健, 田中 珠美, 岡本 好司, 藤田 敏郎

    日本腎臓学会誌　2008/04

  14. 透析患者の循環器合併症治療の最前線 透析患者の末梢動脈疾患の早期発見と治療

    岡本 好司, 守矢 英和, 大竹 剛靖, 野入 英世, 小林 修三

    医工学治療　2008/04

  15. 関連解析によって得られた21番染色体上の2型糖尿病の感受性遺伝子

    岡本 好司, 岩崎 直子, 西村 知紗, 中村 新子, 尾形 真規子, 藤巻 理沙, 鎌谷 直之, 岩本 安彦, 徳永 勝士

    糖尿病　2007/04

  16. 糖尿病合併透析患者の下肢末梢動脈狭窄病変の早期発見 各種検査成績の感度の比較および危険因子

    岡本 好司, 池江 亮太, 守矢 英和, 大竹 剛靖, 浜野 久美子, 小林 修三

    糖尿病　2006/04

  17. ステロイド投与とLDL吸着療法の併用により腎機能の改善を認めた腎コレステロール塞栓症(CCE)の2例

    岡 真知子, 岡本 好司, 守矢 英和, 大竹 剛靖, 小林 修三

    Therapeutic Research　2005/09

    More details Close

    症例1:68歳男.意識消失発作が出現し,CTで胸部大動脈瘤を認め,上行弓部大動脈人工血管置換術を施行した.退院後血清Cr値の上昇を来たし,腎生検で炎症細胞の浸潤を伴う多数のコレステリン結晶を細動脈内に認めた.LDL吸着療法(LDL-A)を計10回施行すると共に,プレドニゾロン30mgの内服を開始した.その結果,Crは3.13mg/dlから2.0mg/dlまで改善した.症例2:62歳男.解離性腹部大動脈瘤および左冠動脈主幹部75%狭窄に対してCABG,人工血管置換術を施行した.退院後Cr,BUNの悪化を来たし,週2回のHD導入となった.腎生検で荒廃糸球体が採取糸球体23個中9個存在し,血管内には多数のコレステリン結晶を認めた.HE染色では血管周囲に炎症細胞の浸潤を,血管内腔壁に泡沫細胞を認めた.腎コレステロール塞栓症の診断でLDL-Aを計10回施行し,プレドニゾロン30mg内服を開始した.その結果,HDを離脱でき,Crは7.8mg/dlから2.5mg/dlまで改善した

  18. ARB単独投与はヒトメサンギウム増殖性腎炎で尿蛋白のみでなく組織学的な改善ももたらすか

    大竹 剛靖, 岡本 好司, 岡 真知子, 真栄里 恭子, 守矢 英和, 小林 修三

    日本腎臓学会誌　2005/05

  19. 透析日/非透析日の違いによる睡眠時無呼吸・睡眠障害の評価

    岡本 好司, 黒岩 園子, 加賀谷 範芳, 田井 香織, 岡 真知子, 真栄里 恭子, 守矢 英和, 高橋 幸成, 大竹 剛靖, 小林 修三

    日本腎臓学会誌　2005/05

  20. 透析患者の血液レオロジーと心血管障害発症の関係

    守矢 英和, 岡 真知子, 岡本 好司, 真栄里 恭子, 麻生 邦子, 大竹 剛靖, 小林 修三

    日本腎臓学会誌　2005/05

  21. ベラプロストナトリウム(BPS:PGI2アナログ)はASO合併透析患者の動脈硬化関連炎症マーカーを抑制する

    守矢 英和, 岡 真知子, 岡本 好司, 真栄里 恭子, 大竹 剛靖, 小林 修三

    日本腎臓学会誌　2005/05

  22. 動脈硬化発症進展に影響を及ぼす白血球血小板複合体及び血液流動性から見たポリスルホン膜とPMMA膜の差異について

    守矢 英和, 岡 真知子, 岡本 好司, 真栄里 恭子, 麻生 邦子, 大竹 剛靖, 小林 修三

    日本透析医学会雑誌　2005/05

  23. 当院透析患者のhospitalizationにおける危険因子の検討

    岡 真知子, 岡本 好司, 真栄里 恭子, 守矢 英和, 大竹 剛靖, 小林 修三

    日本透析医学会雑誌　2005/05

  24. 透析患者の動脈硬化進展抑制 LDL-アフェレーシスの抗炎症作用について

    小林 修三, 岡 真知子, 岡本 好司, 真栄里 恭子, 守矢 英和, 麻生 邦子, 大竹 剛靖

    日本透析医学会雑誌　2005/05

  25. 透析患者における閉塞性動脈硬化症に対する非侵襲的検査方法の比較

    岡本 好司, 岡 真知子, 真栄里 恭子, 守矢 英和, 大竹 剛靖, 小林 修三, 種山 かよ子, 高室 昌司

    日本透析医学会雑誌　2005/05

  26. SPIED法にてHDからPDへ移行した患者の2例

    守矢 英和, 岡 真知子, 岡本 好司, 真栄里 恭子, 大竹 剛靖, 小林 修三

    日本透析医学会雑誌　2005/05

  27. 冠動脈疾患の既往のない末期腎不全患者の透析導入期冠動脈病変と末梢血管病変

    大竹 剛靖, 岡本 好司, 岡 真知子, 真栄里 恭子, 守矢 英和, 麻生 邦子, 小林 修三

    日本透析医学会雑誌　2005/05

  28. 当院における悪性高血圧症例の臨床病理学的検討

    岡 真知子, 岡本 好司, 真栄里 恭子, 大竹 剛靖, 小林 修三

    日本腎臓学会誌　2005/05

  29. 塩酸セベラマーは動脈硬化関連のpro-inflammatory biomarkerを減少させるか

    小林 修三, 岡 真知子, 岡本 好司, 真栄里 恭子, 守矢 英和, 麻生 邦子, 大竹 剛靖

    日本腎臓学会誌　2005/05

  30. ステロイド治療により尿細管機能の改善がみられた原発性胆汁性肝硬変合併のシェーグレン症候群による間質性腎炎の1例

    守矢 英和, 岡 真知子, 岡本 好司, 真栄里 恭子, 大竹 剛靖, 小林 修三

    腎炎症例研究　2005/02

    More details Close

    50歳女.47歳時より慢性甲状腺炎にて加療中であった.3ヵ月に軽度腎機能低下と尿蛋白・血尿を指摘され,以前よりの眼口腔の乾燥症状からシェーングレン症候群を疑われ,今回精査加療目的で入院となった.入院時,代謝性アシドーシスを認め,尿細管評価では近位尿細管でリン酸再吸収低下と汎アミノ酸尿を,遠位尿細管機能で尿酸性化能低下を認めた.免疫化学でIgM高値と自己抗体ではSS-A抗体64倍・SS-B抗体8倍と高値を認めたことから肝生検所見と併せてシェーングレン症候群と慢性甲状腺炎,原発性胆汁性肝硬変の合併と診断した.第1回腎生検での糸球体変化は軽微で,プレドニゾロン(PSL)20mg/日投与により腎機能・肝機能の改善を認めた9ヵ月後にPSLを10mg/日に減量時点で第2回腎生検を施行した.その結果,尿細管間質への細胞浸潤は減少していたが,遠位尿細管に円柱形成を著明に認め,尿細管機能検査では近位尿細管機能の改善を認めたが,汎アミノ酸尿は残存し,尿酸性化能の改善も認めなかった

  31. Short term PD Induction and Education technique(SPIED法)によるPD導入の実績

    守矢 英和, 岡本 好司, 真栄里 恭子, 麻生 邦子, 大竹 剛靖, 小林 修三

    日本透析医学会雑誌　2005/02

    More details Close

    腹膜透析(PD)導入となった患者12名(男9名,女3名,平均年齢61.4歳)を対象とし,短期間で効率的にPDを導入指導する方法(SPIED法)の有用性を検討した.本法は,通常通りのカテーテル挿入とドレーピングを施行した後は,消毒・包交を一切行わずに自宅待機とし,10日後に再入院してクリティカルパスを使用してPDの導入教育をする.入院期間はカテーテル挿入時2.3±0.8日,教育導入時11.5±2.4日であった.術後早期合併症,自宅待機中の緊急事態はみられなかった.平均観察期間21.6±9.9ヵ月の間にみられた長期合併症の頻度については,出口部感染症は1/65.0 患者・月,細菌性腹膜炎は1/260.0 患者・月と良好であり,トンネル感染は認めなかった.本法では,再手術は不要で患者負担は軽減し,出口部を固定させることで術後合併症を抑制し,短期間で効率のよいPDであると考えられた

  32. クレゾールの経皮吸収により生じた急性腎不全の1例

    下山 祐人, 岡本 好司, 岡 真知子, 真栄里 恭子, 守矢 英和, 大竹 剛靖, 小林 修三

    日本内科学会関東地方会　2004/12

  33. 血液透析患者の血圧コントロールにおけるweekly averaged blood pressure(WAB)の有用性と血圧日差変動に関する検討

    守矢 英和, 岡 真知子, 岡本 好司, 真栄里 恭子, 麻生 邦子, 大竹 剛靖, 小林 修三

    日本高血圧学会総会プログラム・抄録集　2004/10

  34. 腎後性腎不全発症を契機に発見され,低補体血症を呈したIgM型MGUSの一例

    守矢 英和, 岡 真知子, 岡本 好司, 真栄里 恭子, 大竹 剛靖, 小林 修三

    日本腎臓学会誌　2004/08

  35. ループス腎炎の治療経過中に非外傷性の頭蓋内出血を繰り返したSLEの1例

    大竹 剛靖, 岡 真知子, 岡本 好司, 真栄里 恭子, 守矢 英和, 麻生 邦子, 小林 修三

    日本腎臓学会誌　2004/08

  36. ステロイド治療が奏功し透析を離脱しえた,尿細管間質病変が主体のMPO-ANCA関連腎炎の1例

    岡本 好司, 岡 真知子, 真栄里 恭子, 守矢 英和, 大竹 剛靖, 小林 修三

    日本腎臓学会誌　2004/08

  37. 下肢閉塞性動脈硬化症における,上腕足関節血圧比(ABPI)・経皮酸素分圧(tcPO2)・皮膚還流圧(skin perfusion pressure,SPP)測定の意義

    守矢 英和, 種山 かよ子, 高室 昌司, 岡本 好司, 真栄里 恭子, 麻生 邦子, 大竹 剛靖, 小林 修三

    日本透析医学会雑誌　2004/05

  38. 透析患者の動脈硬化と末梢血白血球の動態について Micro-channel Array Flow Analyzer(MC-FAN)による検討

    小林 修三, 岡本 好司, 真栄里 恭子, 守矢 英和, 麻生 邦子, 大竹 剛靖

    日本透析医学会雑誌　2004/05

  39. 透析患者の冠動脈石灰化に関する検討

    大竹 剛靖, 岡本 好司, 真栄里 恭子, 守矢 英和, 麻生 邦子, 小林 修三

    日本透析医学会雑誌　2004/05

  40. 軽微な蛋白尿を呈する症例の腎生検組織所見とその後の臨床経過の検討

    岡本 好司, 真栄里 恭子, 守矢 英和, 麻生 邦子, 大竹 剛靖, 小林 修三

    日本腎臓学会誌　2004/04

  41. 維持血液透析患者における心理学的検査(CMI)と脳虚血の関連について

    真栄里 恭子, 岡本 好司, 守矢 英和, 大竹 剛靖, 麻生 邦子, 小林 修三

    日本腎臓学会誌　2004/04

  42. 血液透析患者の血圧コントロールにおけるweekly averaged blood pressure(WAB)の有用性

    守矢 英和, 岡本 好司, 真栄里 恭子, 麻生 邦子, 大竹 剛靖, 小林 修三

    日本腎臓学会誌　2004/04

  43. 狭心症の既往のない末期腎不全患者の透析導入期における冠動脈病変の検討

    大竹 剛靖, 岡本 好司, 真栄里 恭子, 守矢 英和, 麻生 邦子, 小林 修三

    日本腎臓学会誌　2004/04

  44. 透析患者における動脈硬化 慢性炎症と血液レオロジーの関係について

    小林 修三, 岡本 好司, 真栄里 恭子, 守矢 英和, 麻生 邦子, 大竹 剛靖

    日本腎臓学会誌　2004/04

  45. 糸球体血管極周囲を中心に間質の細胞浸潤を伴ったネフローゼ症候群の一例

    岡本 好司, 真栄里 恭子, 守矢 英和, 大竹 剛靖, 小林 修三

    腎炎症例研究　2004/02

    More details Close

    41歳女.15歳時にネフローゼ症候群を初発し,3ヵ月間入院加療を受けた.27歳時に再発し,巣状糸球体硬化症(FGS)の診断でステロイド内服治療を1年間受けた.3週間前から尿量減少,体重増加,浮腫があり受診した.低蛋白血症,低アルブミン血症,蛋白尿などを認め,臨床的には微小変化型ネフローゼ症候群と思われた.腎生検では間質細胞浸潤,上皮細胞剥離,メサンギウム領域へのIgM沈着などを認めFGSの可能性が高いと考えられたが,FGSの典型的所見である分節状糸球体硬化は確認できず,間質性腎炎などの可能性も示唆された

Show all Show first 5

Industrial Property Rights 3

  1. Risk for Nephrotic syndrome

    Property Type: Patent

  2. Risk for Diabetes Mellitus

    Property Type: Patent

  3. Vascular access catheter

    Koji Okamoto

    Property Type: Patent

Research Projects 5

  1. 糖質応答転写因子ChREBPを標的とした糖尿病性腎症の病態解明・新規創薬

    菅原 明, 岩渕 好治, 横山 敦, 岡本 好司

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(B)

    Category: 基盤研究(B)

    Institution: 東北大学

    2020/04 - 2023/03

    More details Close

    我々は当該年度に以下の実験を遂行した。 １）糖尿病性腎症（DN）の発症・増悪におけるChREBPの関与・作用機構の解明：A) CRISPR/Cas9システムを用いて作成したChREBPノックアウトマウスからRNAを抽出してRNAシークエンスに供する準備を進めている。B）東北大学医学部倫理委員会にて承認を得たことから、東北大学病院 腎・高血圧・内分泌科の腎生検組織からのRNA抽出・RNAシークエンスに向けて、DNならびに非DN検体の準備を進めている。 ２）D532のDN改善効果の機序解明および実用化・臨床応用に向けた構造最適化：A）D532の一端を伸長して末端をビオチン化することにより作成したD532プローブを用いて、アビジン‐ビオチン反応を利用したアフィニティ精製・質量分析にてD532の標的因子の同定を進めた結果、ChREBP自身がD532の標的因子であることが明らかとなった。B）ChIPアッセイの結果、D532がChREBPのDNAへの結合を抑制することが認められた。本結果から、D532が高血糖依存性のChREBP転写活性をDNA結合レベルで抑制することが明らかとなった。C）D532はChREBPの核内移行には影響を及ぼさないことが明らかとなった。D）疎水性であるD532をPMB30Wにてミセル化することにより水溶化が出来ることが明らかとなった。本成果により、今後のD532を用いた非臨床試験・臨床試験に向けて大きく前進できるものと期待される。

  2. Deep learning based AI for renal pathology

    Offer Organization: Japan Kidney Association (Miyagi pref)

    System: Research grant-support

    Institution: Tohoku University

    2021/04 - 2022/03

  3. 糖尿病性腎臓病のAI画像診断と網羅発現解析 を使った新規病理診断法の開発

    岡本好司

    Offer Organization: 艮陵医学振興会

    System: 安斎記念糖尿病研究助成

    Institution: 東北大学

    2020/04 - 2021/03

  4. Mechanisms of APOL1 genetic variants in glomerular injury Competitive

    Koji Okamoto

    Offer Organization: Japan Society for the Promotion of Science

    System: Overseas Research Fellowships

    2012/06 - 2014/05

  5. ネフローゼ症候群における蛋白尿発症機構への遺伝学的アプローチと機能的解明 Competitive

    野入英世

    Offer Organization: 日本学術振興会

    System: 科研費 基盤（B）

    2012/04 - 2014/03

Social Activities 1

  1. CROの担当者への講義

    2008/04 - 2009/03

Media Coverage 1

  1. Nikkei Business Publications Medical Advisor

    Nikkei Business Publications

    2018/08/01

    Type: Promotional material