PHOTO

Kenichi Funamoto
Section
Institute of Fluid Science
Job title
Associate Professor
Degree
  • 博士(工学)(東北大学)

  • 修士(情報科学)(東北大学)

e-Rad No.
70451630

Education 3

  • Tohoku University Graduate School, Division of Engineering Department of Bioengineering and Robotics

    - 2007/03/27

  • Tohoku University Graduate School, Division of Information Science Department of System Information Sciences

    - 2005/03

  • Tohoku University Faculty of Engineering Department of Mechanical Electronic Engineering

    - 2002/03

Committee Memberships 7

  • 日本機械学会バイオエンジニアリング部門 制御と情報-生体への応用-研究会 幹事

    2018/04 - Present

  • Journal of Biomechanical Science and Engineering Editor

    2016/04 - 2022/03

  • Technical Section on Data Assimilation for Engineering Design 委員

    2015/04 - 2017/03

  • 日本機械学会計算力学部門「設計に活かすデータ同化研究会」 委員

    2015/04 - 2017/03

  • Journal of Biomechanical Sicence and Engineering 編集委員会 Editor (Public Relations)

    2009/04 - 2016/03

  • Journal of Biomechanical Sicence and Engineering 編集委員会 広報委員

    2009/04 - 2016/03

  • 日本機械学会部門協議会直属分科会「高度物理刺激と生体応答に関する研究分科会(P-SCC12)」 委員

    2013/04 - 2015/03

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Professional Memberships 3

  • Japanese Society for the Study of Cellular slime Molds

    2023/04 - Present

  • The Japan Society of Ultrasonics in Medicine

  • The Japan Society of Mechanical Engineers

Research Interests 6

  • Cell

  • Hypoxia

  • In vivo microenvironment

  • Hemodynamics

  • Measurement-integrated simulation

  • Microfluidic device

Research Areas 4

  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Fluid engineering /

  • Life sciences / Medical systems /

  • Life sciences / Biomaterials /

  • Life sciences / Biomedical engineering /

Awards 13

  1. Outstanding Abstract Award

    2021/12 The 11th Asian-Pacific Conference on Biomechanics (AP Biomech 2021) Migration characteristics of Dictyostelium discoideum depending on oxygen environment

  2. The Young Scientists’ Prize, The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology

    2019/04 Ministry of Education, Culture, Sports, Science and Technology

  3. 2017 JBSE Graphics of the Year Award

    2018/03 Journal of Biomechanical Science and Engineering Effects of upstream bifurcation and bend on the blood flow in a cerebral aneurysm

  4. 2015 JBSE Graphics of the Year Award

    2016/03 Journal of Biomechanical Science and Engineering Investigation of characteristic hemodynamic parameters indicating thinning and thickening sites of cerebral aneurysms

  5. 日本機械学会バイオエンジニアリング部門瀬口賞

    2016/01/09 日本機械学会

  6. 日本超音波医学会奨励賞

    2010/05/10 日本超音波医学会

  7. 日本機械学会奨励賞(研究)

    2007/04/06 日本機械学会

  8. 東北大学工学研究科長賞

    2007/03/26 東北大学

  9. 計測自動制御学会東北支部優秀発表奨励賞

    2006/05/17 計測自動制御学会

  10. 日本機械学会流体工学部門講演会優秀講演賞

    2005/10/29 日本機械学会

  11. 日本機械学会フェロー賞

    2005/01/22 日本機械学会

  12. 計測自動制御学会東北支部優秀発表奨励賞

    2004/12/23 計測自動制御学会

  13. 計測自動制御学会学術奨励賞研究奨励賞

    2004/02/23 計測自動制御学会

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Papers 215

  1. Hypoxia suppresses glucose-induced increases in collective cell migration in vascular endothelial cell monolayers

    Kazuki Sone, Yuka Sakamaki, Satomi Hirose, Mai Inagaki, Masanori Tachikawa, Daisuke Yoshino, Kenichi Funamoto

    Scientific Reports 2024/03/02

    DOI: 10.1038/s41598-024-55706-1  

    ISSN:2045-2322

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    <jats:title>Abstract</jats:title><jats:p>Blood glucose levels fluctuate during daily life, and the oxygen concentration is low compared to the atmosphere. Vascular endothelial cells (ECs) maintain vascular homeostasis by sensing changes in glucose and oxygen concentrations, resulting in collective migration. However, the behaviors of ECs in response to high-glucose and hypoxic environments and the underlying mechanisms remain unclear. In this study, we investigated the collective migration of ECs simultaneously stimulated by changes in glucose and oxygen concentrations. Cell migration in EC monolayer formed inside the media channels of microfluidic devices was observed while varying the glucose and oxygen concentrations. The cell migration increased with increasing glucose concentration under normoxic condition but decreased under hypoxic condition, even in the presence of high glucose levels. In addition, inhibition of mitochondrial function reduced the cell migration regardless of glucose and oxygen concentrations. Thus, oxygen had a greater impact on cell migration than glucose, and aerobic energy production in mitochondria plays an important mechanistic role. These results provide new insights regarding vascular homeostasis relative to glucose and oxygen concentration changes.</jats:p>

  2. Biological characterization of breast cancer spheroid formed by fast fabrication method

    Yuta Iijima, Norino Uenaka, Mayu Morimoto, Daiki Sato, Satomi Hirose, Naoyoshi Sakitani, Masahiro Shinohara, Kenichi Funamoto, Gen Hayase, Daisuke Yoshino

    In vitro models 2024/02/13

    Publisher: Springer Science and Business Media LLC

    DOI: 10.1007/s44164-024-00066-3  

    eISSN:2731-3441

  3. Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans. International-journal Peer-reviewed

    Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Ayumu Konno, Hirokazu Hirai, Taku Saito, Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa, Takamasa Sakai, Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata, Hirotsugu Tsuchimochi, Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura, Kenichi Funamoto, Yasuhiro Sawada

    Nature Biomedical Engineering 2023/07/06

    DOI: 10.1038/s41551-023-01061-x  

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    The mechanisms by which physical exercise benefits brain functions are not fully understood. Here, we show that vertically oscillating head motions mimicking mechanical accelerations experienced during fast walking, light jogging or treadmill running at a moderate velocity reduce the blood pressure of rats and human adults with hypertension. In hypertensive rats, shear stresses of less than 1 Pa resulting from interstitial-fluid flow induced by such passive head motions reduced the expression of the angiotensin II type-1 receptor in astrocytes in the rostral ventrolateral medulla, and the resulting antihypertensive effects were abrogated by hydrogel introduction that inhibited interstitial-fluid movement in the medulla. Our findings suggest that oscillatory mechanical interventions could be used to elicit antihypertensive effects.

  4. The aerotaxis of Dictyostelium discoideum is independent of mitochondria, nitric oxide and oxidative stress

    Satomi Hirose, Julie Hesnard, Nasser Ghazi, Damien Roussel, Yann Voituron, Oliver Cochet-Escartin, Jean-Paul Rieu, Christophe Anjard, Kenichi Funamoto

    Frontiers in Cell and Developmental Biology 11 2023/06/15

    Publisher: Frontiers Media {SA}

    DOI: 10.3389/fcell.2023.1134011  

    ISSN:2296-634X

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    <jats:p>Spatial and temporal variations of oxygen environments affect the behaviors of various cells and are involved in physiological and pathological events. Our previous studies with <jats:italic>Dictyostelium discoideum</jats:italic> as a model of cell motility have demonstrated that aerotaxis toward an oxygen-rich region occurs below 2% O<jats:sub>2</jats:sub>. However, while the aerotaxis of <jats:italic>Dictyostelium</jats:italic> seems to be an effective strategy to search for what is essential for survival, the mechanism underlying this phenomenon is still largely unclear. One hypothesis is that an oxygen concentration gradient generates a secondary oxidative stress gradient that would direct cell migration towards higher oxygen concentration. Such mechanism was inferred but not fully demonstrated to explain the aerotaxis of human tumor cells. Here, we investigated the role on aerotaxis of flavohemoglobins, proteins that can both act as potential oxygen sensors and modulators of nitric oxide and oxidative stress. The migratory behaviors of <jats:italic>Dictyostelium</jats:italic> cells were observed under both self-generated and imposed oxygen gradients. Furthermore, their changes by chemicals generating or preventing oxidative stress were tested. The trajectories of the cells were then analyzed through time-lapse phase-contrast microscopic images. The results indicate that both oxidative and nitrosative stresses are not involved in the aerotaxis of <jats:italic>Dictyostelium</jats:italic> but cause cytotoxic effects that are enhanced upon hypoxia.</jats:p>

  5. Microfluidic platform for the reproduction of hypoxic vascular microenvironments Peer-reviewed

    Naoyuki Takahashi, Daisuke Yoshino, Ryuji Sugahara, Satomi Hirose, Kazuki Sone, Jean-Paul Rieu, Kenichi Funamoto

    Scientific Reports 13 (1) 5428 2023/04/03

    Publisher: Springer Science and Business Media {LLC}

    DOI: 10.1038/s41598-023-32334-9  

    ISSN:2045-2322

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    <jats:title>Abstract</jats:title><jats:p>Vascular endothelial cells (ECs) respond to mechanical stimuli caused by blood flow to maintain vascular homeostasis. Although the oxygen level in vascular microenvironment is lower than the atmospheric one, the cellular dynamics of ECs under hypoxic and flow exposure are not fully understood. Here, we describe a microfluidic platform for the reproduction hypoxic vascular microenvironments. Simultaneous application of hypoxic stress and fluid shear stress to the cultured cells was achieved by integrating a microfluidic device and a flow channel that adjusted the initial oxygen concentration in a cell culture medium. An EC monolayer was then formed on the media channel in the device, and the ECs were observed after exposure to hypoxic and flow conditions. The migration velocity of the ECs immediately increased after flow exposure, especially in the direction opposite to the flow direction, and gradually decreased, resulting in the lowest value under the hypoxic and flow exposure condition. The ECs after 6-h simultaneous exposure to hypoxic stress and fluid shear stress were generally aligned and elongated in the flow direction, with enhanced VE-cadherin expression and actin filament assembly. Thus, the developed microfluidic platform is useful for investigating the dynamics of ECs in vascular microenvironments.</jats:p>

  6. Numerical analysis of hemodynamic changes and blood stagnation in the left ventricle by internal structures and torsional motion Peer-reviewed

    Suguru Miyauchi, Koichi Hosoi, Shingo Tsuda, Toshiyuki Hayase, Kenichi Funamoto

    AIP Advances 13 (4) 045105 2023/04/01

    DOI: 10.1063/5.0143833  

  7. Region-based SVD processing of high-frequency ultrafast ultrasound to visualize cutaneous vascular networks Peer-reviewed

    Anam Bhatti, Takuro Ishii, Naoya Kanno, Hayato Ikeda, Kenichi Funamoto, Yoshifumi Saijo

    Ultrasonics 129 106907-106907 2023/03

    Publisher: Elsevier BV

    DOI: 10.1016/j.ultras.2022.106907  

    ISSN:0041-624X

  8. Development of Ultrasound Phantom Made of Transparent Material: Feasibility of Optical Particle Image Velocimetry International-journal Peer-reviewed

    Muhammad Shiddiq Sayyid Hashuro, Simon Tupin, Narendra Kurnia Putra, Kotaro Daibo, Kosuke Inoue, Takuro Ishii, Hiroyuki Kosukegawa, Kenichi Funamoto, Toshiyuki Hayase, Makoto Ohta

    Ultrasound in Medicine and Biology 49 (6) 1385-1394 2023

    DOI: 10.1016/j.ultrasmedbio.2022.12.020  

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    OBJECTIVE: The need for ultrasound flow phantoms to validate ultrasound systems requires the development of materials that can clearly visualize the flow inside for measurement purposes. METHODS: A transparent ultrasound flow phantom material composed of poly(vinyl alcohol) hydrogel (PVA-H) with dimethyl sulfoxide (DMSO) and water solution manufactured using the freezing method and mixed with quartz glass powder to exhibit scattering effects is proposed. To achieve transparency of the hydrogel phantom, the refractive index (RI) was changed to match that of the glass by modifying the PVA concentration and the ratio of DMSO to water in the solvent. The feasibility of optical particle image velocimetry (PIV) was verified by comparing an acrylic rectangular cross-section channel with a rigid wall. After the feasibility tests, an ultrasound flow phantom was fabricated to conduct ultrasound B-mode visualization and Doppler-PIV comparison. DISCUSSION: The results revealed that the PIV measured through PVA-H material exhibited 0.8% error in the measured maximum velocity compared with PIV through the acrylic material. B-mode images are similar to real tissue visualization with a limitation of a higher sound velocity, when compared with human tissue, of 1792 m/s. Doppler measurement of the phantom revealed approximately 120% and 19% overestimation of maximum and mean velocities, respectively, compared with those from PIV. CONCLUSION: The proposed material possesses the advantage of the single-phantom ability to improve the ultrasound flow phantom for validation of flow.

  9. Stiffness of primordial germ cells is required for their extravasation in avian embryos Peer-reviewed

    Saito, D., Tadokoro, R., Nagasaka, A., Yoshino, D., Teramoto, T., Mizumoto, K., Funamoto, K., Kidokoro, H., Miyata, T., Tamura, K., Takahashi, Y.

    iScience 25 (12) 105629 2022/12

    Publisher: Elsevier BV

    DOI: 10.1016/j.isci.2022.105629  

    ISSN:2589-0042

  10. Brain- Targeted Mechanical Intervention Using Passive Head Motion Can Have an Antihypertensive Effect

    Yasuhiro Sawada, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Keisuke Shinohara, Takuya Kishi, Ayumu Konno, Hirokazu Hirai, Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Toru Ogata, Hirotsugu Tsuchimochi, Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Kenichi Funamoto, Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao

    CIRCULATION 146 2022/11

    Publisher: LIPPINCOTT WILLIAMS & WILKINS

    ISSN:0009-7322

    eISSN:1524-4539

  11. A Review of Functional Analysis of Endothelial Cells in Flow Chambers Invited Peer-reviewed

    Makoto Ohta, Naoya Sakamoto, Kenichi Funamoto, Zi Wang, Yukiko Kojima, Hitomi Anzai

    JOURNAL OF FUNCTIONAL BIOMATERIALS 13 (3) 2022/09

    Publisher: MDPI

    DOI: 10.3390/jfb13030092  

    eISSN:2079-4983

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    The vascular endothelial cells constitute the innermost layer. The cells are exposed to mechanical stress by the flow, causing them to express their functions. To elucidate the functions, methods involving seeding endothelial cells as a layer in a chamber were studied. The chambers are known as parallel plate, T-chamber, step, cone plate, and stretch. The stimulated functions or signals from endothelial cells by flows are extensively connected to other outer layers of arteries or organs. The coculture layer was developed in a chamber to investigate the interaction between smooth muscle cells in the middle layer of the blood vessel wall in vascular physiology and pathology. Additionally, the microfabrication technology used to create a chamber for a microfluidic device involves both mechanical and chemical stimulation of cells to show their dynamics in in vivo microenvironments. The purpose of this study is to summarize the blood flow (flow inducing) for the functions connecting to endothelial cells and blood vessels, and to find directions for future chamber and device developments for further understanding and application of vascular functions. The relationship between chamber design flow, cell layers, and microfluidics was studied.

  12. Comparison of hemodynamics in the left ventricle between tricuspid aortic valve and bicuspid aortic valves Peer-reviewed

    Shingo Tsuda, Suguru Miyauchi, Kenichi Funamoto

    Proceedings of the 13th Pacific Symposium on Flow Visualization and Image Processing (PSFVIP13) PSFVIP13_paper_9 2022/08

  13. Model-based estimation of QT intervals of mouse fetal electrocardiogram. International-journal Peer-reviewed

    Namareq Widatalla, Kiyoe Funamoto, Motoyoshi Kawataki, Chihiro Yoshida, Kenichi Funamoto, Masatoshi Saito, Yoshiyuki Kasahara, Ahsan Khandoker, Yoshitaka Kimura

    Biomedical engineering online 21 (1) 45 2022/06/29

    DOI: 10.1186/s12938-022-01015-5  

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    BACKGROUND: Abnormal prolongation in the QT interval or long QT syndrome (LQTS) is associated with several cardiac complications such as sudden infant death syndrome (SIDS). LQTS is believed to be linked to genetic mutations which can be understood by using animal models, such as mice models. Nevertheless, the research related to fetal QT interval in mice is still limited because of challenges associated with T wave measurements in fetal electrocardiogram (fECG). Reliable measurement of T waves is essential for estimating their end timings for QT interval assessment. RESULTS: A mathematical model was used to estimate QT intervals. Estimated QT intervals were validated with Q-aortic closure (Q-Ac) intervals of Doppler ultrasound (DUS) and comparison between both showed good agreement with a correlation coefficient higher than 0.88 (r > 0.88, P < 0.05). CONCLUSION: Model-based estimation of QT intervals can help in better understanding of QT intervals in fetal mice.

  14. The Oxygen Gradient in Hypoxic Conditions Enhances and Guides <i>Dictyostelium discoideum</i> Migration Invited Peer-reviewed

    Satomi Hirose, Jean-Paul Rieu, Olivier Cochet-Escartin, Christophe Anjard, Kenichi Funamoto

    Processes 10 (2) 318-318 2022/02/07

    Publisher: {MDPI} {AG}

    DOI: 10.3390/pr10020318  

  15. Aerotaxis and aerokinesis of Dictyostelium discoideum under hypoxic microenvironments Peer-reviewed

    Satomi Hirose, Jean-Paul Rieu, Christophe Anjard, Olivier Cochet-Escartin, Haruhisa Kikuchi, Kenichi Funamoto

    Proceedings of 43rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society 2021 (EMBC2021) 2021 1187-1190 2021/11/01

    DOI: 10.1109/EMBC46164.2021.9629752  

    eISSN:2694-0604

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    Although spatiotemporal changes of oxygen in a microenvironment are known to affect the cellular dynamics of various eukaryotes, the details are not fully understood. Here, we describe the aerotaxis and aerokinesis of Dictyostelium discoideum (Dd), which has long been employed as a model organism for eukaryotic cells. We developed a microfluidic device capable of time-lapse observation of cultured cells while controlling oxygen concentrations in microchannels. Migratory behaviors of Dd were observed and quantitatively evaluated under an oxygen concentration gradient from 0% to 21% O2, as well as in various uniform oxygen conditions. In a hypoxic region within the oxygen concentration gradient, Dd migrated toward regions of higher oxygen concentration at increased velocity, which was independent of cell density. Observed under uniform oxygen concentrations of 1%, 2%, 3%, and 21%, the migration velocity of Dd increased significantly in hypoxic environments of 2% O2 or less. Thus, Dd shows aerotaxis, directed by the oxygen concentration gradient, and simultaneously shows aerokinesis, changing the migration velocity according to the oxygen concentration itself.

  16. Reconstruction of 3D human brain microvasculature on a chip using brain endothelial cells, astrocytes and pericytes

    Momoko Sato, Mai Inagaki, Yuka Sakamaki, Kenichi Funamoto, Masanori Tachikawa

    Proceedings of the 18th International Conference on Flow Dynamics (ICFD2021) 495-496 2021/10

  17. Comparison of permeability of 3D microvascular network model under controlled oxygen concentration

    Masataka Nikaido, Tatsuya Osaki, Kenichi Funamoto

    Proceedings of the 18th International Conference on Flow Dynamics (ICFD2021) 493-494 2021/10

  18. Evaluation of migration speed of cancer cells by different types of matrices using microfluidic devices

    Satoshi Aratake, Kenichi Funamoto

    Proceedings of the 18th International Conference on Flow Dynamics (ICFD2021) 489-490 2021/10

  19. Oxygen gradient under severe hypoxia changes Dictyostelium migration directionality

    Satomi Hirose, Jean-Paul Rieu, Christophe Anjard, Olivier Cochet-Escartin, Kenichi Funamoto

    Proceedings of the 18th International Conference on Flow Dynamics (ICFD2021) 487-488 2021/10

  20. Evaluation of PAK1 localization in vascular endothelial cells by hypoxic stresses using microfluidic devices

    Kazuki Sone, Satomi Hirose, Daisuke Yoshino, Kenichi Funamoto

    Proceedings of the 18th International Conference on Flow Dynamics (ICFD2021) 473-474 2021/10

  21. Enhanced Collective migration of endothelial cells in the early stage of exposure to low shear stress

    Ryuji Sugahara, Kenichi Funamoto

    Proceedings of the 18th International Conference on Flow Dynamics (ICFD2021) 471-472 2021/10

  22. Modelling self-organization by oxygen with reaction-diffusion models

    Olivier Cochet-Escartin, Mete Demircigil, Satomi Hirose, Vincent Calvez, Kenichi Funamoto, Christophe Anjard, Jean-Paul Rieu

    Proceedings of the 21st International Symposium on Advanced Fluid Information (AFI-2021) 221-222 2021/10

  23. Science of ultrafine droplet;high speed impact

    Takehiko Sato, Masao Watanabe, Takeru Yano, Yuka Iga, Kazumichi Kobayashi, Atsuki Komiya, Hidemasa Takana, Kiyonobu Ohtani, Junnosuke Okajima, Kenichi Funamoto, Yunchen Xiao, Tomoki Nakajima, Siwei Liu

    Proceedings of the 21st International Symposium on Advanced Fluid Information (AFI-2021) 171-172 2021/10

  24. Numerical analysis of a blood flow in the left ventricle-aorta system

    Suguru Miyauchi, Shuta Kosaka, Toshiyuki Hayase, Kenichi Funamoto

    Proceedings of the 21st International Symposium on Advanced Fluid Information (AFI-2021) 159-160 2021/10

  25. Reconstruction of perfusable human 3D microvasculature on a chip as an evaluation model of cancer cell extravasation and drug transport

    Yuka Sakamaki, Mai Inagaki, Momoko Sato, Kenichi Funamoto, Masanori Tachikawa

    Proceedings of the 21st International Symposium on Advanced Fluid Information (AFI-2021) 158 2021/10

  26. Effects of shear stress on endothelial cell motility

    Kenichi Funamoto, Eugenia Corvera Poiré

    Proceedings of the 21st International Symposium on Advanced Fluid Information (AFI-2021) 156-157 2021/10

  27. Numerical study on the inertial migration and effective viscosity in pressure-driven suspension flows including elliptical particles

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto

    Proceedings of the 21st International Symposium on Advanced Fluid Information (AFI-2021) 154-155 2021/10

  28. Elucidation of a blood turbulence using electronic stethoscope

    Masatsugu Hirano, Hikaru Dalton Yukimura, Katsuhito Yamasaki, Kenichi Funamoto

    Proceedings of the 21st International Symposium on Advanced Fluid Information (AFI-2021) 152-153 2021/10

  29. Hypoxia triggers collective aerotactic migration in dictyostelium discoideum Peer-reviewed

    Cochet-Escartin, O., Demircigil, M., Hirose, S., Allais, B., Gonzalo, P., Mikaelian, I., Funamoto, K., Anjard, C., Calvez, V., Rieu, J.-P.

    eLife 10 e64731 2021/08/20

    DOI: 10.7554/eLife.64731  

    ISSN:2050-084X

  30. Reduced oxygen availability triggers aerotaxis and aerokinesis of Dictyostelium International-coauthorship

    Satomi Hirose, Olivier Cochet-Escartin, Christophe Anjard, Jean-Paul Rieu, Kenichi Funamoto

    Proceedings of the LyonSE&N & ELyT Global Workshop 2021 31-32 2021/06

  31. P21-activated kinase regulates oxygen-dependent migration of vascular endothelial cells in monolayers Peer-reviewed

    Hirose, S., Tabata, Y., Sone, K., Takahashi, N., Yoshino, D., Funamoto, K.

    Cell Adhesion and Migration 15 (1) 272-284 2021/01/01

    Publisher: Informa {UK} Limited

    DOI: 10.1080/19336918.2021.1978368  

    ISSN:1933-6926 1933-6918

  32. Hydrostatic pressure promotes endothelial tube formation through aquaporin 1 and Ras-ERK signaling Peer-reviewed

    Daisuke Yoshino, Kenichi Funamoto, Kakeru Sato, Kenry, Masaaki Sato, Chwee Teck Lim

    Communications Biology 3 (1) 2020/12/01

    DOI: 10.1038/s42003-020-0881-9  

    eISSN:2399-3642

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    © 2020, The Author(s). Vascular tubulogenesis is tightly linked with physiological and pathological events in the living body. Endothelial cells (ECs), which are constantly exposed to hemodynamic forces, play a key role in tubulogenesis. Hydrostatic pressure in particular has been shown to elicit biophysical and biochemical responses leading to EC-mediated tubulogenesis. However, the relationship between tubulogenesis and hydrostatic pressure remains to be elucidated. Here, we propose a specific mechanism through which hydrostatic pressure promotes tubulogenesis. We show that pressure exposure transiently activates the Ras/extracellular signal-regulated kinase (ERK) pathway in ECs, inducing endothelial tubulogenic responses. Water efflux through aquaporin 1 and activation of protein kinase C via specific G protein–coupled receptors are essential to the pressure-induced transient activation of the Ras/ERK pathway. Our approach could provide a basis for elucidating the mechanopathology of tubulogenesis-related diseases and the development of mechanotherapies for improving human health.

  33. Cancer cell migration and cancer drug screening in oxygen tension gradient chip Peer-reviewed

    Nam, H., Funamoto, K., Jeon, J.S.

    Biomicrofluidics 14 (4) 044107-044107 2020/07

    Publisher: AIP Publishing

    DOI: 10.1063/5.0011216  

    ISSN:1932-1058

    eISSN:1932-1058

  34. Microfluidic platform for three-dimensional cell culture under spatiotemporal heterogeneity of oxygen tension Peer-reviewed

    Rei Koens, Yugo Tabata, Jean C. Serrano, Satoshi Aratake, Daisuke Yoshino, Roger D. Kamm, Kenichi Funamoto

    APL Bioengineering 4 (1) 2020/03/01

    DOI: 10.1063/1.5127069  

    eISSN:2473-2877

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    © 2020 Author(s). Cells in a tumor microenvironment are exposed to spatial and temporal variations in oxygen tension due to hyperproliferation and immature vascularization. Such spatiotemporal oxygen heterogeneity affects the behavior of cancer cells, leading to cancer growth and metastasis, and thus, it is essential to clarify the cellular responses of cancer cells to oxygen tension. Herein, we describe a new double-layer microfluidic device allowing the control of oxygen tension and the behavior of cancer cells under spatiotemporal oxygen heterogeneity. Two parallel gas channels were located above the media and gel channels to enhance gas exchange, and a gas-impermeable polycarbonate film was embedded in the device to prevent the diffusion of atmospheric oxygen. Variations in oxygen tension in the device with the experimental parameters and design variables were investigated computationally and validated by using oxygen-sensitive nanoparticles. The present device can generate a uniform hypoxic condition at oxygen levels down to 0.3% O2, as well as a linear oxygen gradient from 3% O2 to 17% O2 across the gel channel within 15 min. Moreover, human breast cancer cells suspended in type I collagen gel were introduced in the gel channel to observe their response under controlled oxygen tension. Hypoxic exposure activated the proliferation and motility of the cells, which showed a local maximum increase at 5% O2. Under the oxygen gradient condition, the increase in the cell number was relatively high in the central mild hypoxia region. These findings demonstrate the utility of the present device to study cellular responses in an oxygen-controlled microenvironment.

  35. Measurement of Particle Concentration Profiles of a Dilute Suspension in Different Reynolds Number Conditions

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto, Miho Tanaka, Mitsuru Tanaka, Shigeru Murata, Suguru Miyauchi

    Proceedings of 19th International Symposium on Advanced Fluid Information 164-165 2019/11

  36. Viscosity estimation of a suspension with rigid spheres in circular microchannels using particle tracking velocimetry Peer-reviewed

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto, Miho Tanaka, Mitsuru Tanaka, Shigeru Murata, Suguru Miyauchi, Toshiyuki Hayase

    Micromachines 10 (10) 2019/10/01

    DOI: 10.3390/mi10100675  

    eISSN:2072-666X

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    © 2019 by the authors. Suspension flows are ubiquitous in industry and nature. Therefore, it is important to understand the rheological properties of a suspension. The key to understanding the mechanism of suspension rheology is considering changes in its microstructure. It is difficult to evaluate the influence of change in the microstructure on the rheological properties affected by the macroscopic flow field for non-colloidal particles. In this study, we propose a new method to evaluate the changes in both the microstructure and rheological properties of a suspension using particle tracking velocimetry (PTV) and a power-law fluid model. Dilute suspension (0.38%) flows with fluorescent particles in a microchannel with a circular cross section were measured under low Reynolds number conditions (Re ≈ 10-4). Furthermore, the distribution of suspended particles in the radial direction was obtained from the measured images. Based on the power-law index and dependence of relative viscosity on the shear rate, we observed that the non-Newtonian properties of the suspension showed shear-thinning. This method will be useful in revealing the relationship between microstructural changes in a suspension and its rheology.

  37. Numerical analysis of the blood flow in the left ventricle with internal structures: Effect of trabeculae carneae models and atrial fibrillation Peer-reviewed

    Suguru Miyauchi, Tomomi Yamada, Koichi Hosoi, Toshiyuki Hayase, Kenichi Funamoto

    AIP Advances 9 (10) 105209-1-105209-10 2019/10/01

    DOI: 10.1063/1.5124730  

    eISSN:2158-3226

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    © 2019 Author(s). Internal structures of the inner wall of the left ventricle (LV), such as trabeculae carneae (TC) and papillary muscles, may reduce the blood flow velocity, resulting in thrombus formation in the LV of a healthy heart or a heart with atrial fibrillation. The purpose of the present study was to numerically clarify the effects of simplified TC models around the LV wall and atrial fibrillation on the blood flow field as a fundamental study. The LV model was constructed from magnetic resonance images, and the model considering atrial fibrillation was constructed based on the volume change data of a healthy subject from which volume increase with atrial contraction was removed. Numerical analysis was performed considering eight simplified TC models around the LV wall. The numerical results revealed that the oscillatory shear index and relative residence time (RRT) at the apex in the LV model with internal structures were larger than those in the LV model without internal structures, which indicates that the blood flow tends to stagnate. On the other hand, as a result of the LV model considering the atrial fibrillation, the time-averaged wall shear stress decreases and the RRT increases at valley points of the internal structures.

  38. Oxygen-dependent contraction and degradation of the extracellular matrix mediated by interaction between tumor and endothelial cells Peer-reviewed

    Yoshino, D., Funamoto, K.

    AIP Advances 9 (4) 045215-1-045215-1-10 2019/04

    DOI: 10.1063/1.5089772  

    ISSN:2158-3226

  39. Experimental study on the effects of radial dispersion of spherical particles on the suspension rheology Peer-reviewed

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto, Suguru Miyauchi, Toshiyuki Hayase

    ASME-JSME-KSME 2019 8th Joint Fluids Engineering Conference, AJKFluids 2019 5 2019

    DOI: 10.1115/AJKFluids2019-5322  

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    Copyright © 2019 JSME. Experimental studies were performed to characterize the effects of the microstructure on the rheology of suspension. We focused on the change in the dispersion of the suspended particles under different particle Reynolds number conditions. Suspension flow through a microchannel with a circular cross-section was measured, and the radial dispersion of suspended particles and the velocity profiles were obtained. It was suggested that the particle dispersion could be changed owing to the difference in inertial force acting on the particles.

  40. Migration of vascular endothelial cells in monolayers under hypoxic exposure International-journal Peer-reviewed

    Yugo Tabata, Daisuke Yoshino, Kiyoe Funamoto, Rei Koens, Roger D Kamm, Kenichi Funamoto

    Integrative Biology 11 (1) 26-35 2019/01

    Publisher: Oxford University Press ({OUP})

    DOI: 10.1093/intbio/zyz002  

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    The hypoxic microenvironment existing in vivo is known to significantly affect cell morphology and dynamics, and cell group behaviour. Collective migration of vascular endothelial cells is essential for vasculogenesis and angiogenesis, and for maintenance of monolayer integrity. Although hypoxic stress increases vascular endothelial permeability, the changes in collective migration and intracellular junction morphology of vascular endothelial cells remain poorly understood. This study reveals the migration of confluent vascular endothelial cells and changes in their adherens junction, as reflected by changes in the vascular endothelial (VE)-cadherin distribution, under hypoxic exposure. Vascular endothelial monolayers of human umbilical vein endothelial cells (HUVECs) were formed in microfluidic devices with controllability of oxygen tension. The oxygen tension was set to either normoxia (21% O2) or hypoxia (<3% O2) by supplying gas mixtures into separate gas channels. The migration velocity of HUVECs was measured using particle image velocimetry with a time series of phase-contrast microscopic images of the vascular endothelial monolayers. Hypoxia inducible factor-1α (HIF-1α) and VE-cadherin in HUVECs were observed after exposure to normoxic or hypoxic conditions using immunofluorescence staining and quantitative confocal image analysis. Changes in the migration speed of HUVECs were observed in as little as one hour after exposure to hypoxic condition, showing that the migration speed was increased 1.4-fold under hypoxia compared to that under normoxia. Nuclear translocation of HIF-1α peaked after the hypoxic gas mixture was supplied for 2 h. VE-cadherin expression was also found to be reduced. When ethanol was added to the cell culture medium, cell migration increased. By contrast, by strengthening VE-cadherin junctions with forskolin, cell migration decreased gradually in spite the effect of ethanol to stimulate migration. These results indicate that the increase of cell migration by hypoxic exposure was attributable to loosening of intercellular junction resulting from the decrease of VE-cadherin expression.

  41. Numerical and experimental studies on non-Newtonian rheology of a suspension

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto, Suguru Miyauchi, Toshiyuki Hayase

    Proceedings of the 18th International Symposium on Advanced Fluid Information 2018/11

  42. Initial response of cell-cell junction in endothelial monolayer to hypoxic exposure

    FUNAMOTO Kenichi, YOSHINO Daisuke, NAKAYAMA Masafumi

    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2018.30 1A11 2018

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmebio.2018.30.1a11  

    eISSN:2424-2829

  43. Numerical analysis of the effect of trabeculae carneae models on blood flow in a left ventricle model constructed from magnetic resonance images Peer-reviewed

    Tomomi Yamada, Toshiyuki Hayase, Suguru Miyauchi, Kenichi Funamoto

    Journal of Biomechanical Science and Engineering 13 (2) 17-00597-1-17-00597-14 2018

    DOI: 10.1299/jbse.17-00597  

    ISSN:1880-9863

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    © 2018 The Japan Society of Mechanical Engineers. Although the blood flow velocity in a left ventricle (LV) has been considered to be sufficiently fast to prevent thrombus formation, internal wall structures, such as trabeculae carneae (TC) and papillary muscle, recently received attention as possible causes of reduced near-wall blood flow. As a fundamental consideration of this problem, this study established a method for constructing an unsteady LV model from magnetic resonance (MR) images and investigated the effect of a few simplified TC structures on the blood flow in the model. The LV model at arbitrary time steps was constructed by deforming a computational mesh generated from MR images at a reference time step. The validity of the proposed construction scheme was confirmed by comparison with the configuration of an LV model extracted from MR images. Numerical analysis was performed for the unsteady blood flow in LV models with and without two simplified TC structures. The flow field in the model with the internal structure differed from that in the model without the internal structure near the wall, and flow separation caused by the internal structure decreased wall shear stress on the rear of the internal structure. The computational results provide fundamental information for the complex interaction between the internal structures and the blood flow in an LV.

  44. Microfluidic device for co-culture under controlled oxygen tension

    Kenichi Funamoto, Daisuke Yoshino

    Abstract book of the 3rd International Symposium on Mechanobiology (ISMB2017) 242 2017/12/11

  45. Estimation of viscosity profiles of semidilute suspensions by computational and experimental studies

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto, Toshiyuki Hayase

    Proceedings of the 17th International Symposium on Advanced Fluid Information (AFI-2017) 78-79 2017/11/01

  46. Endothelial monolayer permeability under controlled oxygen tension Peer-reviewed

    Kenichi Funamoto, Daisuke Yoshino, Kento Matsubara, Ioannis K. Zervantonakis, Kiyoe Funamoto, Masafumi Nakayama, Jun Masamune, Yoshitaka Kimura, Roger D. Kamm

    INTEGRATIVE BIOLOGY 9 (6) 529-538 2017/06

    Publisher: ROYAL SOC CHEMISTRY

    DOI: 10.1039/c7ib00068e  

    ISSN:1757-9694

    eISSN:1757-9708

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    Endothelial permeability has been extensively investigated in the context of pathologies such as cancer and also in studies of drug delivery from the circulation. Hypoxia is a critical regulator of endothelial cell (EC) behavior and affects the barrier function of endothelial linings, yet its role has been little studied. This paper reveals the effect of hypoxia on the permeability of an EC monolayer by cellular experiments using a microfluidic device and a conventional cell culture dish. Human umbilical vein endothelial cells (HUVECs) were seeded into one microfluidic channel, creating an EC monolayer on each vertical surface of a collagen gel confined to a central chamber. Oxygen tension was regulated to produce normoxic (21% O-2) or hypoxic (3% O-2) conditions by the supply of gas mixtures of oxygen, carbon dioxide, and nitrogen at predefined ratios into channels fabricated into the device. Permeability of the EC monolayer quantified by analyzing diffusion of fluorescence-labelled dextrans into the collagen gel increases with barrier function loss by 6 hour hypoxic exposure, showing 11-fold and 4-fold increases for 70 kDa and 10 kDa dextrans, respectively, on average. Consistent with this, subsequent immunofluorescent staining and separate western blot analysis of HUVECs on a culture dish demonstrate loose cell-cell adhesion resulting from internalization of VE-cadherin under hypoxia. Thus, hypoxic stress increases endothelial permeability by altering cell-cell junction integrity.

  47. Photoplethysmography and ultrasonic-measurement-integrated simulation to clarify the relation between two-dimensional unsteady blood flow field and forward and backward waves in a carotid artery Peer-reviewed

    Shusaku Sone, Toshiyuki Hayase, Kenichi Funamoto, Atsushi Shirai

    MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING 55 (5) 719-731 2017/05

    Publisher: SPRINGER HEIDELBERG

    DOI: 10.1007/s11517-016-1543-4  

    ISSN:0140-0118

    eISSN:1741-0444

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    Understanding the spatiotemporal change in hemodynamics is essential for the basic research of atherosclerosis. The objective of this study was to establish a methodology to clarify the relation between a two-dimensional (2D) unsteady blood flow field and forward and backward propagating waves in a carotid artery. This study utilized photoplethysmography (PPG) for blood pressure measurement and two-dimensional ultrasonic-measurement-integrated (2D-UMI) simulation for flow field analysis. The validity of the methodology was confirmed in an experiment for a carotid artery of a healthy volunteer. Synchronization between the pressure measurement and flow field analysis was achieved with an error of &lt; 10 ms. A 2D unsteady blood flow field in the carotid artery was characterized in relation to forward and backward waves. 2D-UMI simulation reproduced the flow field in which the wall shear stress takes a maximum at the time of the backward wave superiority in the systolic phase, whereas 2D ordinary simulation failed to reproduce this feature because of poor reproducibility of velocity distribution. In conclusion, the proposed methodology using PPG and 2D-UMI simulation was shown to be a potential tool to clarify the relation between 2D unsteady blood flow field and the forward and backward waves in a carotid artery.

  48. Ultrasound Imaging of Mouse Fetal Intracranial Hemorrhage Due to Ischemia/Reperfusion Peer-reviewed

    Kenichi Funamoto, Takuya Ito, Kiyoe Funamoto, Clarissa L. Velayo, Yoshitaka Kimura

    FRONTIERS IN PHYSIOLOGY 8 (340) 1-6 2017/05

    Publisher: FRONTIERS MEDIA SA

    DOI: 10.3389/fphys.2017.00340  

    ISSN:1664-042X

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    Despite vast improvement in perinatal care during the 30 years, the incidence rate of neonatal encephalopathy remains unchanged without any further Progress towards preventive strategies for the clinical impasse. Antenatal brain injury including fetal intracranial hemorrhage caused by ischemia/reperfusion is known as one of the primary triggers of neonatal injury. However, the mechanisms of antenatal brain injury are poorly understood unless better predictive models of the disease are developed. Here we show a mouse model for fetal intracranial hemorrhage in vivo developed to investigate the actual timing of hypoxia-ischemic events and their related mechanisms of injury. Intrauterine growth restriction mouse fetuses were exposed to ischemia/reperfusion cycles by occluding and opening the uterine and ovarian arteries in the mother. The presence and timing of fetal intracranial hemorrhage caused by the ischemia/reperfusion were measured with histological observation and ultrasound imaging. Protein restricted diet increased the risk of fetal intracranial hemorrhage. The monitoring of fetal brains by ultrasound B-mode imaging clarified that cerebral hemorrhage in the fetal brain occurred after the second ischemic period. Three-dimensional ultrasound power Doppler imaging visualized the disappearance of main blood flows in the fetal brain. These indicate a breakdown of cerebrovascular autoregulation which causes the fetal intracranial hemorrhage. This study supports the fact that the ischemia/reperfusion triggers cerebral hemorrhage in the fetal brain. The present method enables us to noninvasively create the cerebral hemorrhage in a fetus without directly touching the body but with repeated occlusion and opening of the uterine and ovarian arteries in the mother.

  49. Deformation of stenotic blood vessel model made from Poly (Vinyl Alcohol) Hydrogel by hydrostatic pressure Peer-reviewed

    Yasutomo Shimizu, Lei Liu, Hiroyuki Kosukegawa, Kenichi Funamoto, Toshiyuki Hayase, Toshio Nakayama, Makoto Ohta

    PROCEEDINGS OF THE ASME INTERNATIONAL MECHANICAL ENGINEERING CONGRESS AND EXPOSITION, 2016, VOL. 3 V003T04A055 2017

    Publisher: AMER SOC MECHANICAL ENGINEERS

    DOI: 10.1115/IMECE2016-66657  

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    Vascular plaque deformation reduces blood flow, increases arterial embolism risk, and may lead to ischemic stroke. Plaque stiffness varies widely and is an important factor influencing both plaque and parent artery deformation. These geometric changes affect local hemodynamics, which impact plaque initiation influencing disease progression. However, most previous studies used non-elastic stenotic vessel models. For more realistic analysis, we constructed a stenosis model comprising an elastic poly (vinyl alcohol) hydrogel (PVA-H) parent artery and plaque of variable stiffness. Our previous study using this flexible model demonstrated substantial effects of hydrostatic pressure. Here ultrasonography was conducted under changing hydrostatic pressure to measure geometric changes at the narrowest cross section. PVA-H specimens were constructed with the stiffness of a hard lipid core, smooth muscle, and plaque, as estimated by tensile tests using 5, 12, and 15 wt% PVA, respectively. The change in cross-sectional aspect ratio (height/face length) at the narrowest site is largest (similar to 1.3) for the 5 wt% PVA-H plaque and smallest (similar to 1.2) for the 12 wt% PVA-H plaque. Stenotic artery deformation depends on both artery and plaque elasticity. Hydrostatic pressure has a substantial effect on both vessel and plaque geometries, which markedly alter blood flow.

  50. Effects of upstream bifurcation and bend on the blood flow in a cerebral aneurysm Peer-reviewed

    Daichi Suzuki, Kenichi Funamoto, Shin-ichiro Sugiyama, Toshiyuki Hayase, Suguru Miyauchi, Teiji Tominaga

    Journal of Biomechanical Science and Engineering 12 (4) 17-00189-1-17-00189-11 2017

    Publisher: Japan Society of Mechanical Engineers

    DOI: 10.1299/jbse.17-00189  

    ISSN:1880-9863

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    Mechanisms of development, growth, and rupture of a cerebral aneurysm have been studied by computational fluid dynamics (CFD), calculating hemodynamic parameters, such as wall shear stress, oscillatory shear index (OSI), and relative residence time (RRT). It is well known that the upstream velocity profile and upstream vessel shape influence the computational results. However, few studies have dealt with cases involving a bifurcation upstream of a cerebral aneurysm. Furthermore, the fluid-dynamic effects of multiple structural elements of upstream vessel shape, such as a bifurcation and a bend, on the results of CFD remain unknown. The purpose of this study was to elucidate the fluid-dynamic effects of multiple structural elements of upstream vessel shape such as bifurcation and bend on blood flow and hemodynamic parameters inside an aneurysm. Computations were performed for blood flow in a cerebral aneurysm with upstream sequential elements of a bifurcation, a bend, and a straight section, using four models with different upstream boundaries, i.e., before the bifurcation, after the bifurcation before the bend, after the bend, and after the straight section just before the cerebral aneurysm. The results were compared to elucidate the effect of each upstream structural element on the blood flow and hemodynamic parameters in the cerebral aneurysm. Differences in OSI and RRT resulting from changes in the velocity distribution were observed locally in the aneurysm between the models. It was also found that the effect of the bifurcation on the velocity distribution was greater than that of the bend.

  51. Numerical analysis for elucidation of mechanical interaction between an erythrocyte moving in medium subject to inclined centrifugal force and endothelial cells on a plate Peer-reviewed

    Akira Yatsuyanagi, Toshiyuki Hayase, Suguru Miyauchi, Kenichi Funamoto, Kosuke Inoue, Atsushi Shirai, Luca Brandt

    Journal of Fluid Science and Technology 11 (4) JFST0029 2016/12/28

    DOI: 10.1299/jfst.2016jfst0029  

  52. Daughter Sac Formation Related to Blood Inflow Jet in an Intracranial Aneurysm Peer-reviewed

    Sin-ichiro Sugiyama, Hidenori Endo, Shunsuke Omodaka, Toshiki Endo, Kuniyasu Niizuma, Sherif Rashad, Toshio Nakayama, Kenichi Funamoto, Makoto Ohta, Teiji Tominaga

    WORLD NEUROSURGERY 96 396-402 2016/12

    Publisher: ELSEVIER SCIENCE INC

    DOI: 10.1016/j.wneu.2016.09.040  

    ISSN:1878-8750

    eISSN:1878-8769

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    OBJECTIVE: We performed a hemodynamic study of an intracranial aneurysm with a newly developed daughter sac during observation to investigate the role of hemodynamics on the formation of a daughter sac. METHODS: A 75-year-old man underwent magnetic resonance angiography that revealed a large internal carotid artery aneurysm with inflow jet inside the aneurysm. The aneurysm was stable for 18 months, but a new daughter sac developed at the tip of the aneurysm during the next 6 months. The daughter sac seemed to be related to the inflow jet on magnetic resonance angiography. Aneurysm geometries before and after daughter sac formation were reconstructed using the longitudinal data of magnetic resonance angiography. Computational fluid dynamic simulations were conducted under the patient-specific pulsatile inlet conditions measured by magnetic resonance velocimetry. RESULTS: The hemodynamic simulation revealed that the inflow jet impinged on 2 sites of the aneurysm: the right side of the aneurysmal dome and the tip of the aneurysm. The flow impingement caused elevation of pressure at both sites. However, the daughter sac formed at the latter site surrounded by the basal cistern but did not form at the former site that was in contact with the right temporal lobe. CONCLUSIONS: Blood inflow jet caused local elevation of pressure, and the formation of the daughter sac occurred at the site with high pressure but without the surrounding structure, which may cancel the perpendicular wall tension.

  53. Microfluidic experiments of cellular responses to hypoxia Invited

    Kenichi Funamoto

    Proceedings of the 13th International Conference on Flow Dynamics (ICFD2016) 324-325 2016/10/11

  54. Preliminary study for fluid dynamic effects of upstream bifurcation and bend on blood flow simulation in cerebral aneurysm

    Daichi Suzuki, Kenichi Funamoto, Shin-ichiro Sugiyama, Toshiyuki Hayase, Suguru Miyauchi, Teiji Tominaga

    Proceedings of the 13th International Conference on Flow Dynamics (ICFD2016) 264-265 2016/10/11

  55. Analysis of an autonomic nervous system of mouse fetus with congenital heart defect

    Kiyoe Funamoto, Rika Sugibayashi, Kenichi Funamoto, Kana Nakanishi, Takuya Ito, Motoyoshi Kawataki, Toshiyuki Hayase, Yoshitaka Kimura

    Proceedings of the 16th International Symposium on Advanced Fluid Information (AFI-2016) 92-93 2016/10/11

  56. Computational and experimental studies on the blood cells behavior in microcirculation

    Tomohiro Fukui, Misa Kawaguchi, Atsuhide Kitagawa, Kenichi Funamoto, Toshiyuki Hayase

    Proceedings of the 16th International Symposium on Advanced Fluid Information (AFI-2016) 82-83 2016/10/11

  57. Computational investigation toward selective collection of water particles containing odorous molecules by electrostatic spraying Peer-reviewed

    Jin Muraoka, Kenichi Funamoto, Mariko Seno, Satoshi Arimoto, Ken Shimono, Satoshi Suzuki, Yoshio Mitsutake, Tetsuya Maekawa, Toshihiko Yoshioka, Toshiyuki Hayase

    JOURNAL OF ELECTROSTATICS 83 35-41 2016/10

    Publisher: ELSEVIER SCIENCE BV

    DOI: 10.1016/j.elstat.2016.07.004  

    ISSN:0304-3886

    eISSN:1873-5738

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    The necessity of odor sensing has been increasing from environmental and health standpoints. Here, we propose the novel concept of a small device which can select odor molecules based on electrostatic spraying. For high selectivity of the target gas or odor, we conducted computational fluid dynamics coupled with an electrostatic field, as well as measurements by particle image velocimetry and anemometry. The computational model successfully reproduced characteristic features of ionic wind. Different trajectories of charged particles were computationally obtained owing to their electrical mobility. The results imply that different materials might be separated by the arrangement of the collecting electrode. (C) 2016 Elsevier B.V. All rights reserved.

  58. NOVEL REDUCED UTERINE PERFUSION PRESSURE (RUPP) MODEL OF PREECLAMPSIA IN MICE Peer-reviewed

    Fushima Tomofumi, Sekimoto Akiyo, Minato Takahiro, Ito Takuya, Oe Yuji, Kisu Kiyomi, Sato Emiko, Funamoto Kenichi, Kimura Yoshitaka, Ito Sadayoshi, Sato Hiroshi, Takahashi Nobuyuki

    JOURNAL OF HYPERTENSION 34 E237 2016/09

    DOI: 10.1097/01.hjh.0000500527.66210.66  

    ISSN:0263-6352

  59. Effects of inflow velocity profile on two-dimensional hemodynamic analysis by ordinary and ultrasonic-measurement-integrated simulations Peer-reviewed

    Takaumi Kato, Shusaku Sone, Kenichi Funamoto, Toshiyuki Hayase, Hiroko Kadowaki, Nobuyuki Taniguchi

    MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING 54 (9) 1331-1339 2016/09

    Publisher: SPRINGER HEIDELBERG

    DOI: 10.1007/s11517-015-1376-6  

    ISSN:0140-0118

    eISSN:1741-0444

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    Two-dimensional ultrasonic-measurement-integrated (2D-UMI) simulation correctly reproduces hemodynamics even with an inexact inflow velocity distribution. This study aimed to investigate which is superior, a two-dimensional ordinary (2D-O) simulation with an accurate inflow velocity distribution or a 2D-UMI simulation with an inaccurate one. 2D-O and 2D-UMI simulations were performed for blood flow in a carotid artery with four upstream velocity boundary conditions: a velocity profile with backprojected measured Doppler velocities (condition A), and velocity profiles with a measured Doppler velocity distribution, a parabolic one, and a uniform one, magnitude being obtained by inflow velocity estimation (conditions B, C, and D, respectively). The error of Doppler velocity against the measurement data was sensitive to the inflow velocity distribution in the 2D-O simulation, but not in the 2D-UMI simulation with the inflow velocity estimation. Among the results in conditions B, C, and D, the error in the worst 2D-UMI simulation with condition D was 31 % of that in the best 2D-O simulation with condition B, implying the superiority of the 2D-UMI simulation with an inaccurate inflow velocity distribution over the 2D-O simulation with an exact one. Condition A resulted in a larger error than the other conditions in both the 2D-O and 2D-UMI simulations.

  60. Reduced Uterine Perfusion Pressure (RUPP) Model of Preeclampsia in Mice Peer-reviewed

    Tomofumi Fushima, Akiyo Sekimoto, Takahiro Minato, Takuya Ito, Yuji Oe, Kiyomi Kisu, Emiko Sato, Kenichi Funamoto, Toshiyuki Hayase, Yoshitaka Kimura, Sadayoshi Ito, Hiroshi Sato, Nobuyuki Takahashi

    PLOS ONE 11 (5) e0155426 2016/05

    Publisher: PUBLIC LIBRARY SCIENCE

    DOI: 10.1371/journal.pone.0155426  

    ISSN:1932-6203

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    Preeclampsia (PE) is a pregnancy-induced hypertension with proteinuria that typically develops after 20 weeks of gestation. A reduction in uterine blood flow causes placental ischemia and placental release of anti-angiogenic factors such as sFlt-1 followed by PE. Although the reduced uterine perfusion pressure (RUPP) model is widely used in rats, investigating the role of genes on PE using genetically engineered animals has been problematic because it has been difficult to make a useful RUPP model in mice. To establish a RUPP model of PE in mice, we bilaterally ligated ovarian vessels distal to ovarian branches, uterine vessels, or both in ICR-strain mice at 14.5 days post coitum (dpc). Consequently, these mice had elevated BP, increased urinary albumin excretion, severe endotheliosis, and mesangial expansion. They also had an increased incidence of miscarriage and premature delivery. Embryonic weight at 18.5 dpc was significantly lower than that in sham mice. The closer to the ligation site the embryos were, the higher the resorption rate and the lower the embryonic weight. The phenotype was more severe in the order of ligation at the ovarian vessels &lt; uterine vessels &lt; both. Unlike the RUPP models described in the literature, this model did not constrict the abdominal aorta, which allowed BP to be measured with a tail cuff. This novel RUPP model in mice should be useful for investigating the pathogenesis of PE in genetically engineered mice and for evaluating new therapies for PE.

  61. Properties of convective delivery in spinal cord gray matter: laboratory investigation and computational simulations Peer-reviewed

    Toshiki Endo, Yushi Fujii, Shin-ichiro Sugiyama, Rong Zhang, Shogo Ogita, Kenichi Funamoto, Ryuta Saito, Teiji Tominaga

    JOURNAL OF NEUROSURGERY-SPINE 24 (2) 359-366 2016/02

    Publisher: AMER ASSOC NEUROLOGICAL SURGEONS

    DOI: 10.3171/2015.5.SPINE141148  

    ISSN:1547-5654

    eISSN:1547-5646

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    OBJECTIVE Convection-enhanced delivery (CED) is a method for distributing small and large molecules locally into the interstitial space of the spinal cord. Delivering these molecules to the spinal cord is otherwise difficult due to the blood spinal cord barrier. Previous research has proven the efficacy of CED for delivering molecules over long distances along the white matter tracts in the spinal cord. Conversely, the characteristics of CED for delivering molecules to the gray matter of the spinal cord remain unknown. The purpose of this study was to reveal regional distribution of macromolecules in the gray and white matter of the spinal cord with special attention to the differences between the gray and white matter. METHODS Sixteen rats (F344) underwent Evans blue dye CED to either the white matter (dorsal column, 8 rats) or the gray matter (ventral horn, 8 rats) of the spinal cord. The rates and total volumes of infusion were 0.2 mu l/min and 2.0 mu l, respectively. The infused volume of distribution was visualized and quantified histologically. Computational models of the rat spinal cord were also obtained to perform CED simulations in the white and gray matter. RESULTS The ratio of the volume of distribution to the volume of infusion in the gray matter of the spinal cord was 3.60 +/- 0.69, which was comparable to that of the white matter (3.05 +/- 0.88). When molecules were injected into the white matter, drugs remained in the white matter tract and rarely infused into the adjacent gray matter. Conversely, when drugs were injected into the gray matter, they infiltrated laterally into the white matter tract and traveled longitudinally and preferably along the white matter. In the infusion center, the areas were larger in the gray matter CED than in the white matter (Mann-Whitney U-test, p &lt; 0.01). In computational simulations, the aforementioned characteristics of CED to the gray and white matter were reaffirmed. CONCLUSIONS In the spinal cord, the gray and white matter have distinct characteristics of drug distribution by CED. These differences between the gray and white matter should be taken into account when considering drug delivery to the spinal cord. Computational simulation is a useful tool for predicting drug distributions in the normal spinal cord.

  62. Study of Estimation Method for Unsteady Inflow Velocity in Two-Dimensional Ultrasonic-Measurement-Integrated Blood Flow Simulation Peer-reviewed

    Hiroko Kadowaki, Toshiyuki Hayase, Kenichi Funamoto, Nobuyuki Taniguchi

    IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING 63 (2) 403-414 2016/02

    Publisher: IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC

    DOI: 10.1109/TBME.2015.2461559  

    ISSN:0018-9294

    eISSN:1558-2531

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    Information on hemodynamics is essential for elucidation of mechanisms and development of novel diagnostic methods for circulatory diseases. Two-dimensional ultrasonic-measurement-integrated (2D-UMI) simulation can correctly reproduce an intravascular blood flow field and hemodynamics by feeding back an ultrasonic measurement to the numerical blood flow simulation. In this method, it is critically important to give the correct cross-sectional average inflow velocity (inflow velocity) as the boundary condition. However, systematic study has not been done on the relative validity and effectiveness of existing inflow velocity estimation methods for various target flow fields. The aim of this study was to examine the existing methods systematically and to establish a method to accurately estimate inflow velocities for various vessel geometries and flow conditions in 2D-UMI simulations. A numerical experiment was performed for 2D-UMI simulation of blood flow models in a straight vessel with inflow velocity profiles symmetric and asymmetric to the vessel axis using existing evaluation functions based on Doppler velocity error for the inflow velocity estimation. As a result, it was clarified that a significantly large estimation error occurs in the asymmetric flow due to a nonfeedback domain near the downstream end of the calculation domain. Hence, a new inflow velocity estimation method of 2DUMI simulation is proposed in which the feedback and evaluation domains are extended to the downstream end. Further numerical experiments of 2D-UMI simulation for two realistic vessel geometries of a healthy blood vessel and a stenosed one confirmed the effectiveness of the proposed method.

  63. Computational Hemodynamic Analysis for the Diagnosis of Atherosclerotic Changes in Intracranial Aneurysms: A Proof-of-Concept Study Using 3 Cases Harboring Atherosclerotic and Nonatherosclerotic Aneurysms Simultaneously Peer-reviewed

    Shin-ichiro Sugiyama, Hidenori Endo, Kuniyasu Niizuma, Toshiki Endo, Kenichi Funamoto, Makoto Ohta, Teiji Tominaga

    COMPUTATIONAL AND MATHEMATICAL METHODS IN MEDICINE 2386031 2016

    Publisher: HINDAWI PUBLISHING CORP

    DOI: 10.1155/2016/2386031  

    ISSN:1748-670X

    eISSN:1748-6718

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    This was a proof-of-concept computational fluid dynamics (CFD) study designed to identify atherosclerotic changes in intracranial aneurysms. We selected 3 patients with multiple unruptured aneurysms including at least one with atherosclerotic changes and investigated whether an image-based CFD study could provide useful information for discriminating the atherosclerotic aneurysms. Patient-specific geometries were constructed from three-dimensional data obtained using rotational angiography. Transient simulations were conducted under patient-specific inlet flow rates measured by phase-contrast magnetic resonance velocimetry. In the postanalyses, we calculated time-averaged wall shear stress (WSS), oscillatory shear index, and relative residence time (RRT). The volume of blood flow entering aneurysms through the neck and the mean velocity of blood flow inside aneurysms were examined. We applied the age-of-fluid method to quantitatively assess the residence of blood inside aneurysms. Atherosclerotic changes coincided with regions exposed to disturbed blood flow, as indicated by low WSS and long RRT. Blood entered aneurysms in phase with inlet flow rates. The mean velocities of blood inside atherosclerotic aneurysms were lower than those inside nonatherosclerotic aneurysms. Blood in atherosclerotic aneurysms was older than that in nonatherosclerotic aneurysms, especially near the wall. This proof-of-concept study demonstrated that CFD analysis provided detailed information on the exchange and residence of blood that is useful for the diagnosis of atherosclerotic changes in intracranial aneurysms.

  64. Study of Permeability Changes of Endothelial Cell Monolayer Exposed to Hypoxia

    K. Matsubara, K. Funamoto, I.K. Zervantonakis, K. Funamoto, T. Ito, Y. Kimura, T. Hayase, R.D. Kamm

    Proceedings of the 12th International Conference on Flow Dynamics (ICFD2015) 362-363 2015/10/29

  65. Determination of Optimum Feedback Gain of Two-Dimensional Ultrasonic-Measurement-Integrated Blood Flow Analysis System

    H. Kadowaki, T. Hayase, K. Funamoto, S. Miyauchi, K. Inoue, T. Shimazaki, T. Jibiki, K. Miyama

    Proceedings of the 12th International Conference on Flow Dynamics (ICFD2015) 460-461 2015/10/29

  66. Fundamental Study of Interaction between Erythrocyte and Endothelial Cell under Inclined Centrifugal Force (Physical Explanation of Interaction Model)

    A. Yatsuyagagi, T. Hayase, S. Miyauchi, K. Funamoto, K. Inoue, A. Shirai, L. Brandt

    Proceedings of 12th International Conference on Flow Dynamics (ICFD2015) 320-321 2015/10/28

  67. Numerical Analysis of Ultrasound Scattering Property of Medium Mimicking Blood (Influence of Ultrasound Frequency)

    Y. Chiba, T. Hayase, S. Miyauchi, K. Funamoto

    Proceedings of the 12th International Conference on Flow Dynamics (ICFD2015) 308-309 2015/10/28

  68. Cardiac Evaluation of Fetal Mice by ECG and Ultrasound

    R. Sugibayashi, T. Ito, K. Funamoto, T. Hayase, Y. Kimura

    Proceedings of the 15th International Symposium on Advanced Fluid Information (AFI-2015) 184-185 2015/10/28

  69. Investigation of Inlet Position in Hemodynamic Analysis of a Cerebral Aneurysm

    D. Suzuki, K. Funamoto, S. Sugiyama, T. Hayase, S. Miyauchi, T. Tominaga

    Proceedings of the 12th International Conference on Flow Dynamics (ICFD2015) 304-305 2015/10/27

  70. Effect of Speckle Noise in Ultrasonic Measurement on Two-Dimensional Ultrasonic-Measurement-Integrated Blood Flow Analysis Peer-reviewed

    H. Kadowaki, T. Hayase, K. Funamoto, S. Miyauchi, K. Inoue, T. Shimazaki, T. Jibiki, K. Miyama

    Proceedings of the World Congress on Electrical Engineering and Computer Systems and Science (EECSS 2015) 323-1-323-8 2015/07/15

  71. Measurements of Blood Flow and Blood Pressure under Different Indoor Temperature and Body Postural Conditions, and Development of a New Human Simulation Model Peer-reviewed

    H. Sakamoto, Y. Chiba, T. Hayase, K. Funamoto, Y. Saijo, T. Goto

    Healthy Buildings Europe 2015 437-1-437-8 2015/05/18

  72. Numerical analysis of hemodynamic changes in the left atrium due to atrial fibrillation Peer-reviewed

    Ryo Koizumi, Kenichi Funamoto, Toshiyuki Hayase, Yusuke Kanke, Muneichi Shibata, Yasuyuki Shiraishi, Tomoyuki Yambe

    JOURNAL OF BIOMECHANICS 48 (3) 472-478 2015/02

    Publisher: ELSEVIER SCI LTD

    DOI: 10.1016/j.jbiomech.2014.12.025  

    ISSN:0021-9290

    eISSN:1873-2380

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    Atrial fibrillation (AF) disrupts movement of the left atrium (IA) and worsens the vital prognosis by causing thromboembolism. Ultrasound Doppler measurement, phase-contrast magnetic resonance imaging (PC MRI), as well as computational fluid dynamics (CFD) have revealed hemodynamic changes in the LA due to AF, such as stagnation of blood flow in the left atrial appendage (LAA). However, quantitative evaluation of the hemodynamics during AF has not been conducted, and the effects of important AF characteristics, such as a lack of active contraction of the LA (atrial kick) in late diastole and the occurrence of high-frequency fibrillation (&gt; 400 bpm) of the atrial wall, on blood flow field and concomitant hemodynamic stresses have not been completely understood. In this study, the effects of the above-mentioned two characteristic phenomena of AF on blood flow and hemodynamic parameters were quantitatively investigated. Based on MRI of a healthy volunteer heart, one healthy LA model and two AF models (one without atrial kick, and one without atrial kick and with high-frequency fibrillation) were constructed to perform hemodynamic analysis, and the computational results were compared. The results revealed that each characteristic phenomenon of AF influenced hemodynamics. Especially, atrial wall movement by high-frequency fibrillation had a large impact on the stagnation of blood flow. The relative residence time (RRT), which is an indicator of stagnation of blood flow, increased in the upper part of the LAA during AF. This result implies that there is a local thrombus-prone site in LAA when AF occurs. (C) 2014 Elsevier Ltd. All rights reserved.

  73. Microscopic observation of glass bead movement in soft tissue-mimicking phantom under ultrasound PW mode scanning Peer-reviewed

    Lei Liu, Kenichi Funamoto, Masayuki Tanabe, Toshiyuki Hayase

    JOURNAL OF MEDICAL ULTRASONICS 42 (1) 59-63 2015/01

    Publisher: SPRINGER JAPAN KK

    DOI: 10.1007/s10396-014-0565-5  

    ISSN:1346-4523

    eISSN:1613-2254

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    Previous studies have demonstrated that stones and calcification in soft tissue show special enhancement in response to color flow (CF) or pulse Doppler (PW) mode ultrasound scan. This phenomenon is known as the "twinkling sign (TS)". The authors conducted an in vitro experiment to investigate the mechanism of TS occurrence by observing a glass bead in a transparent PVA-H soft tissue-mimicking phantom. The TS in PW mode showed a low-power and slow-velocity spectrum. At the same time, analysis of images by high-speed camera showed that the glass bead in the phantom oscillated following the pulse repetition frequency (PRF) of the PW mode ultrasound scan. The harmonic oscillations were confirmed, as well. The ultrasound radiation force-driven micro-oscillation possibly affects the ultrasound propagation around the scatterer and triggers random signals in the received echo signals. The results indicate that TS is a phenomenon based on complicated acoustic-mechanical interaction of multiple mechanisms. Further investigation is required for gaining a full understanding of the mechanism of TS occurrence and its clinical application.

  74. Poly(vinyl alcohol) gel ultrasound phantom with durability and visibility of internal flow Peer-reviewed

    Kenichi Funamoto, Osamu Yamashita, Toshiyuki Hayase

    JOURNAL OF MEDICAL ULTRASONICS 42 (1) 17-23 2015/01

    Publisher: SPRINGER JAPAN KK

    DOI: 10.1007/s10396-014-0560-x  

    ISSN:1346-4523

    eISSN:1613-2254

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    Among various existing flow phantoms, none is characterized by appropriate acoustic, visibility, and durability properties simultaneously. The aim of this study was to develop a durable ultrasound phantom with visibility of the internal flow. Poly(vinyl alcohol) (PVA) gel was chosen as the basic material. The acoustic properties of various PVA gels were measured with 40-MHz ultrasound, the compositions of PVA, dimethyl sulfoxide (DMSO), and glass microbeads being changed, while visually checking the transparency. Wall-less ultrasound flow phantoms with a straight channel 2 mm in diameter were made from PVA gel, and ultrasound B-mode imaging was conducted with blood-mimicking fluid flow. The acoustic properties of in vivo soft tissue were reproduced by PVA gel with a PVA concentration of 15 mass% and a glass microbead concentration of 2.9 mass% in a solvent of 98 mol% DMSO, showing acoustic properties of 1567 +/- A 4 m/s and 56 +/- A 5 dB/cm. The PVA gel was durable with visibility of the flow in the ultrasound phantom. The ultrasound B-mode image of the ultrasound flow phantom showed features approximating those of a mouse carotid artery. A durable PVA gel ultrasound phantom with visibility of the internal flow was developed.

  75. Blood flow analysis in carotid artery bifurcation by two-dimensional ultrasonic-measurement-integrated simulation Peer-reviewed

    Hiroko Kadowaki, Toshiyuki Hayase, Kenichi Funamoto, Shusaku Sone, Tadashi Shimazaki, Takao Jibiki, Koji Miyama

    Journal of Biomechanical Science and Engineering 10 (1) 1-14 2015

    Publisher: Japan Society of Mechanical Engineers

    DOI: 10.1299/jbse.14-00266  

    ISSN:1880-9863

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    If highly precise elucidation of the blood flow characteristics in a carotid bifurcation was possible, it would be widely applicable to diagnosis of circulatory diseases such as arteriosclerosis and cerebrovascular disease. This study was conducted to establish a new flow-dividing ratio estimation method applicable to an unsteady flow on a two-dimensional ultrasonic-measurement-integrated simulation of a carotid artery bifurcation for which it has been previously difficult to obtain a stable solution. In this new method, the flow-dividing ratio was directly adjusted by specifying the flow rate in a branch so that the difference of the Doppler velocities in the external carotid artery was decreased. The effectiveness of the proposed method was confirmed by a numerical experiment using the actual shape of a carotid artery bifurcation, and the superiority of the two-dimensional ultrasonic-measurement-integrated simulation over the ordinary simulation in terms of the reproducibility of the blood flow structure was clarified by analysis using clinical ultrasound data.

  76. Investigation of characteristic hemodynamic parameters indicating thinning and thickening sites of cerebral aneurysms Peer-reviewed

    Daichi Suzuki, Kenichi Funamoto, Shinichiro Sugiyama, Toshio Nakayama, Toshiyuki Hayase, Teiji Tominaga

    Journal of Biomechanical Science and Engineering 10 (1) 1-10 2015

    Publisher: Japan Society of Mechanical Engineers

    DOI: 10.1299/jbse.14-00265  

    ISSN:1880-9863

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    Cellular and animal experiments and computational fluid dynamics (CFD) have revealed that mechanisms of the initiation, growth and rupture of a cerebral aneurysm are related to hemodynamics. By direct observation of a cerebral aneurysm during craniotomy, thinning or thickening sites can be found on the aneurysmal wall. The thinning site of a cerebral aneurysm is considered to be at high risk of rupture. In addition, the thickening site of a cerebral aneurysm is not necessarily in a stable state since arteriosclerosis may have occurred. Hence, information on wall conditions, i.e., thinning and thickening, of a cerebral aneurysm is beneficial for clinical diagnosis and treatment. In this study, a hemodynamic parameter to effectively estimate the thinness or thickness of cerebral aneurysmal walls was investigated. CFD of hemodynamics in cerebral aneurysms developed at the anterior communicating artery (ACoA), a common site of cerebral aneurysms, was performed, and characteristic distributions of hemodynamic parameters were investigated by comparing the computational results with clinical images. As a result, a high value of the time-averaged wall shear stress (TAWSS) was found to be present at thinning sites, while a low TAWSS and a high relative residence time (RRT) of an indicator of blood retention were observed at thickening sites. Thinning and thickening sites each have their own characteristics distribution of hemodynamic parameters.

  77. Numerical Analysis for Elucidation of Nonlinear Frictional Characteristics of a Deformed Erythrocyte Moving on a Plate in Medium Subject to Inclined Centrifugal Force Peer-reviewed

    Takashi Oshibe, Toshiyuki Hayase, Kenichi Funamoto, Atsushi Shirai

    JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME 136 (12) 121003-1-121003-9 2014/12

    Publisher: ASME

    DOI: 10.1115/1.4028723  

    ISSN:0148-0731

    eISSN:1528-8951

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    Complex interactions between blood cells, plasma proteins, and glycocalyx in the endothelial surface layer are crucial in microcirculation. To obtain measurement data of such interactions, we have previously performed experiments using an inclined centrifuge microscope, which revealed that the nonlinear velocity-friction characteristics of erythrocytes moving on an endothelia-cultured glass plate in medium under inclined centrifugal force are much larger than those on plain or material-coated glass plates. The purpose of this study was to elucidate the nonlinear frictional characteristics of an erythrocyte on plain or material-coated glass plates as the basis to clarify the interaction between the erythrocyte and the endothelial cells. We propose a model in which steady motion of the cell is realized as an equilibrium state of the force and moment due to inclined centrifugal force and hydrodynamic flow force acting on the cell. Other electrochemical effects on the surfaces of the erythrocyte and the plate are ignored for the sake of simplicity. Numerical analysis was performed for a three-dimensional flow of a mixture of plasma and saline around a rigid erythrocyte model of an undeformed biconcave shape and a deformed shape with a concave top surface and a flat bottom surface. A variety of conditions for the concentration of plasma in a medium, the velocity of the cell, and the minimum gap width and the angle of attack of the cell from the plate, were examined to obtain the equilibrium states. A simple flat plate model based on the lubrication theory was also examined to elucidate the physical meaning of the model. The equilibrium angle of attack was obtained only for the deformed cell model and was represented as a power function of the minimum gap width. A simple flat plate model qualitatively explains the power function relation of the frictional characteristics, but it cannot explain the equilibrium relation, confirming the computational result that the deformation of the cell is necessary for the equilibrium. The frictional characteristics obtained from the present computation qualitatively agree with those of former experiments, showing the validity of the proposed model.

  78. Development and feasibility study of a two-dimensional ultrasonic-measurement-integrated blood flow analysis system for hemodynamics in carotid arteries Peer-reviewed

    Takaumi Kato, Kenichi Funamoto, Toshiyuki Hayase, Shusaku Sone, Hiroko Kadowaki, Tadashi Shimazaki, Takao Jibiki, Koji Miyama, Lei Liu

    MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING 52 (11) 933-943 2014/11

    Publisher: SPRINGER HEIDELBERG

    DOI: 10.1007/s11517-014-1193-3  

    ISSN:0140-0118

    eISSN:1741-0444

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    Prevention and early detection of atherosclerosis are critical for protection against subsequent circulatory disease. In this study, an automated two-dimensional ultrasonic-measurement-integrated (2D-UMI) blood flow analysis system for clinical diagnosis was developed, and the feasibility of the system for hemodynamic analysis in a carotid artery was revealed. The system automatically generated a 2D computational domain based on ultrasound color Doppler imaging and performed a UMI simulation of blood flow field to visualize hemodynamics in the domain. In the UMI simulation, compensation of errors was applied by adding feedback signals proportional to the differences between Doppler velocities by measurement and computation while automatically estimating the cross-sectional average inflow velocity. The necessity of adjustment of the feedback gain was examined by analyzing blood flow in five carotid arteries: three healthy, one sclerosed, and one stenosed. The same feedback gain was generally applicable for the 2D-UMI simulation in all carotid arteries, depending on target variables. Thus, the present system was shown to be versatile in the sense that the parameter is patient independent. Moreover, the possibility of a new diagnostic method based on the hemodynamic information obtained by the 2D-UMI simulation, such as a waveform of the cross-sectional average inflow velocity and wall shear stress distributions, was suggested.

  79. Analysis of High Reynolds Number Flow behind a Square Cylinder by Hybrid Wind Tunnel (Improvement of Critical Gain of Instability for Pressure Feedback)

    Jumpei Okutani, Toshiyuki Hayase, Kosuke Inoue, Shusaku Sone, Kenichi Funamoto

    Proceedings of the Eleventh International Conference on Flow Dynamics (ICFD2014) 270-271 2014/10/10

  80. Application of MR-Measurement-Integrated Hemodynamic Simulation to Cerebrovascular Diseases

    S. Sugiyama, K. Funamoto, D. Suzuki, T. Hayase, T. Tominaga

    Proceedings of the 14th International Symposium on Advanced Fluid Information (AFI-2014) 104-105 2014/10/09

  81. Cardiac Evaluation of Fetal Mice by ECG and Ultrasound

    R. Sugibayashi, T. Ito, K. Funamoto, T. Hayase, Y. Kimura

    Proceedings of the 14th International Symposium on Advanced Fluid Information (AFI-2014) 94-95 2014/10/09

  82. Elucidation of Mechanisms of the Frictional Characteristics of Erythrocytes under Inclined Centrifugal Force

    K. Funamoto, L. Brandt, A. Yatsuyanagi, K. Inoue, T. Hayase

    Proceedings of the 14th International Symposium on Advanced Fluid Information (AFI-2014) 100-101 2014/10/09

  83. Evaluation of Permeability of Endothelial Cell Monolayer under Controlled Oxygen Tension

    K. Funamoto, I.K. Zervantonakis, K. Matsubara, K. Funamoto, T. Ito, Y. Kimura, R.D. Kamm

    Proceedings of the 14th International Symposium on Advanced Fluid Information (AFI-2014) 102-103 2014/10/09

  84. Investigation on Advanced Medical Ultrasound Imaging Technology

    M. Tanabe, H. Hashimoto, K. Funamoto, Y. Chiba, T. Hayase

    Proceedings of the 14th International Symposium on Advanced Fluid Information (AFI-2014) 192-193 2014/10/09

  85. Observation of Hypoxia Cellular Response by Using Microfluidic Devices

    S. Fukushima, K. Funamoto

    Proceedings of the 14th International Symposium on Advanced Fluid Information (AFI-2014) 98-99 2014/10/09

  86. Fundamental Study of Interaction between Erythrocyte and Endothelial Cell under Inclined Centrifugal Force (Reproduction of Friction Characteristics by Numerical Analysis Using Simple Interaction Model)

    Akira Yatsuyanagi, Toshiyuki Hayase, Kenichi Funamoto, Kosuke Inoue, Atsushi Shirai

    Proceedings of the Eleventh International Conference on Flow Dynamics (ICFD2014) 296-297 2014/10/08

  87. Studies on the basic technology of “Sono-cytometry” Peer-reviewed

    Yosuke Komatsu, Ryo Nagaoka, Kenichi Funamoto, Toshiyuki Hayase, Nobuo Masauzi, Hiroshi Kanai, Yoshifumi Saijo

    Transactions of Japanese Society for Medical and Biological Engineering 52 351-O-352 2014/08/17

    Publisher: Japan Soc. of Med. Electronics and Biol. Engineering

    DOI: 10.11239/jsmbe.52.O-351  

    ISSN:1347-443X 1881-4379

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    Flow cytometry is widely used to classify individual cells optically but blood sampling is required for cell measurement in blood. If the principle of the cytometry is applicable directly in vivo, diagnosis of malaria would become easier. However, optical observation of the cell in blood flow is difficult because absorption and scattering of the light is large in vivo. In this study, novel ultrasonic method called "Sono-cytometry" for differentiating cells is proposed. The backscatter signal from the sphere with the diameter of 8 and 10-micron were detected by a transducer with the central frequency of 75 MHz. When the theoretical value is calculated from Faran-Hickling Model, linear approximate slope matched with theoretical slope. When calculating the slope of theoretical value by changing the spherical diameter, the slope value was very different between 8 and 10 micron. The result shows that it is possible that this method can classify spheres.

  88. Simultaneous Analysis System for Blood Pressure and Flow Using Ultrasonic-Measurement-Integrated Simulation

    Shusaku Sone, Toshiyuki Hayase, Kenichi Funamoto, Atsushi Shirai

    Abstracts of the 7th World Congress of Biomechanics F5 2014/07/11

  89. Observation of Extracellular Collagen Remodeling by Second-Harmonic-Generation Microscopy

    S. Fukushima, R. Maehara, T. Araki, K. Funamoto, R.D. Kamm

    Abstracts of the 7th World Congress of Biomechanics 2014/07/06

  90. 超音波計測融合シミュレーションにおける血流量推定精度の向上

    門脇 弘子, 早瀬 敏幸, 船本 健一, 曾根 周作, 島崎 正, 地挽 隆夫, 見山 広二, 劉 磊

    超音波医学 41 (Suppl.) S480-S480 2014/04

    Publisher: (公社)日本超音波医学会

    ISSN:1346-1176

    eISSN:1881-9311

  91. 光電脈派計測と超音波計測融合解析による血圧血流同時計測システムによるWI解析

    曾根 周作, 早瀬 敏幸, 船本 健一, 白井 敦

    超音波医学 41 (Suppl.) S480-S480 2014/04

    Publisher: (公社)日本超音波医学会

    ISSN:1346-1176

    eISSN:1881-9311

  92. 2B47 Consideration on the evaluation function of blood flow rate estimation in ultrasonic-measurement-integrated simulation

    KADOWAKI Hiroko, HAYASE Toshiyuki, FUNAMOTO Kenichi, SONE Shusaku, JIBIKI Takao, HASHIMOTO Hiroshi, MIYAMA Koji, LIU Lei

    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2014 337-338 2014

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmebio.2014.26.337  

  93. 2B26 Development of Simultaneous Analysis System for Blood Pressure and Flow Using Photoplethysmography and Ultrasonic-Measurement-Integrated Simulation : Evaluation of Wave Intensity Using Developed System

    Shusaku SONE, Toshiyuki HAYASE, Kenichi FUNAMOTO, Atsushi SHIRAI

    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2014.26 (0) 311-312 2014

    Publisher: Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmebio.2014.26.311  

    eISSN:2424-2829

  94. 107 Analysis of High Reynolds Number Flow Behind a Square Cylinder by Hybrid Wind Tunnel : Improvement of Critical Gain of Instability for Pressure Feedback

    Jumpei OKUTANI, Toshiyuki HAYASE, Kosuke INOUE, Shusaku SONE, Kenichi FUNAMOTO

    The Proceedings of Conference of Tohoku Branch 2014.49 (0) 15-16 2014

    Publisher: Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmeth.2014.49.15  

    eISSN:2424-2713

  95. Hemodynamic analysis of intracranial aneurysms with atherosclerosis Peer-reviewed

    Shinichiro Sugiyama, K. Funamoto, T. Nakayama, K. Niizuma, T. Tominaga

    IFMBE Proceedings 43 738-740 2014

    Publisher: Springer Verlag

    DOI: 10.1007/978-3-319-02913-9_188  

    ISSN:1680-0737

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    Intracranial aneurysms can have atherosclerotic wall properties that may be important in predicting aneurysm history. This study aimed to investigate hemodynamic characteristics of atherosclerotic legions in intracranial aneurysms. We conducted computational fluid dynamic analyses of 30 aneurysms using patient-derived geometries and inlet flow rates. Among 30 aneurysms, seven atherosclerotic lesions with remarkable yellow lipid deposition were identified in five aneurysms. All seven atherosclerotic lesions were spatially agreed with the area exposed to stagnant blood flow. Univariate analysis revealed that male (P = 0.031), cigarette smoking (P = 0.047) and the exposure to stagnant blood flow (P = 0.024) are significantly related to atherosclerotic lesion formation on the aneurysmal wall. Of those variables that influenced atherogenesis, the variable male (P = 0.0046) and the exposure to stagnant flow (P = 0.0037) remained significant in the multivariate regression model. In conclusion, male sex and stagnant blood flow inside aneurysms were independent risk factors for atherosclerosis in intracranial aneurysms.

  96. "Sonocytometry" - Novel Diagnostic Method of Ultrasonic Differentiation of Cells in Blood Flow Peer-reviewed

    Yosuke Komatsu, Ryo Nagaoka, Ken-ichi Funamoto, Toshiyuki Hayase, Nobuo Masauzi, Hiroshi Kanai, Yoshifumi Saijo

    2014 36TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) 2761-2764 2014

    Publisher: IEEE

    DOI: 10.1109/EMBC.2014.6944195  

    ISSN:1557-170X

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    Novel diagnostic method named "sonocytometry", in which streaming blood cell is diagnosed by the reflection of high frequency ultrasound from the cell, is proposed. In the present study, the differentiation of the particle size is performed as a basic study on sonocytometry. Ultrasonic backscatter signal from either 80 or 100 mu m diameter polystyrene particles was measured by an ultrasonic transducer with the central frequency of 30 MHz. The spectrum of the reflected signal showed different characteristics according to the particle diameter. Theoretical value of backscatter was calculated by Faran-Hickling model and the correlation coefficient of measured and theoretical value by varying the spherical diameter showed the local maximum value at either 80 or 100 mu m diameter. The principle was also validated on the streaming particles in a flow channel. The method successfully classified the particle size. Sonocytometry would be clinically applied for diagnosis of malaria or leukemia.

  97. Effects of Time-Varying Feedback Signals on Pressure Field in Ultrasonic-Measurement-Integrated Simulation of Pulsatile Blood Flow Peer-reviewed

    K. Funamoto, T. Hayase

    Proceedings of the 3rd International Conference on Computational and Mathematical Biomedical Engineering (CMBE13) 295-298 2013/12/17

  98. Cardiac Evaluation of Fetal Mice by ECG and Ultrasound

    T. Ito, K. Funamoto, R. Sugibayashi, K. Funamoto, C. Velayo, M. Endo, Y. Dong, T. Hayase, Y. Kimura

    Proceedings of the 13th International Symposium on Advanced Fluid Information (AFI-2013) 60-61 2013/11/26

  99. Development of a Microfluidic Device for a Three-Dimensional Cell Culture under a Controlled Hypoxic Environment

    K. Funamoto, I. K. Zervantonakis, R. D. Kamm

    Proceedings of the 13th International Symposium on Advanced Fluid Information (AFI-2013) 184-185 2013/11/26

  100. Effects of Temporal and Spatial Oxygen Heterogeneity on Cell Processes

    K. Funamoto, I. K. Zervantonakis, K. Funamoto, T. Ito, Y. Kimura, R. D. Kamm

    Proceedings of the 13th International Symposium on Advanced Fluid Information (AFI-2013) 92-93 2013/11/26

  101. Evaluation of Intracranial Aneurysm Rupture Using MR-Measurement-Integrated Simulation

    S. Sugiyama, K. Funamoto, D. Suzuki, T. Hayase, T. Tominaga

    Proceedings of the 13th International Symposium on Advanced Fluid Information (AFI-2013) 86-87 2013/11/26

  102. In-Depth Investigation of Twinkling Sign: Optical Observation of Ultrasound Radiation Force Driven Oscillation of Glass Particle

    L. Liu, K. Funamoto, M. Tanabe, T. Hayase

    Proceedings of the 13th International Symposium on Advanced Fluid Information (AFI-2013) 58-59 2013/11/26

  103. Numerical Simulation of Ultrasound Imaging for Detection of Microcalcification in Soft Tissue

    M. Tanabe, E. Tagomori, L. Liu, K. Funamoto, M. Nishimoto, T. Hayase

    Proceedings of the 13th International Symposium on Advanced Fluid Information (AFI-2013) 62-63 2013/11/26

  104. Observation of Hypoxia Cellular Response by Using Microfluidic Devices

    S. Fukushima, R. Maehara, K. Funamoto

    Proceedings of the 13th International Symposium on Advanced Fluid Information (AFI-2013) 90-91 2013/11/26

  105. Improvement of Simultaneous Analysis System for Blood Pressure and Flow Velocity Using Photoplethymography and Ultrasonic-Measurement-Integrated Simulation

    Shusaku Sone, Toshiyuki Hayase, Kenichi Funamoto, Atsushi Shirai

    Proceedings of the 7th East Asian Consortium on Biomedical Engineering 66-67 2013/11/18

  106. Consideration on the Evaluation Function of Blood Flow Rate Estimation in Ultrasonic-Measurement-Integrated Simulation

    Hiroko Kadowaki, Toshiyuki Hayase, Kenichi Funamoto, Shusaku Sone, Takao Jibiki, Hiroshi Hashimoto, Koji Miyama, Lei Liu

    Proceedings of 7th East Asian Consortium on Biomedical Engineering 64-65 2013/11/18

  107. Stagnant Blood Flow in Intracranial Aneurysms: A Possible Association with Atherosclerosis

    Shin-ichiro Sugiyama, Toshio Nakayama, Kenichi Funamoto, Daichi Suzuki, Kuniyasu Niizuma, Makoto Ohta, Teiji Tominaga

    Tenth International Conference on Flow Dynamics Proceedings 592-593 2013/11

  108. Computational simulation of convection-enhanced drug delivery in the non-human primate brainstem: a simple model predicting the drug distribution Peer-reviewed

    Shin-ichiro Sugiyama, Ryuta Saito, Kenichi Funamoto, Toshio Nakayama, Yukihiko Sonoda, Yoji Yamashita, Tomoo Inoue, Toshihiro Kumabe, Toshiyuki Hayase, Teiji Tominaga

    NEUROLOGICAL RESEARCH 35 (8) 773-781 2013/10

    Publisher: MANEY PUBLISHING

    DOI: 10.1179/1743132813Y.0000000215  

    ISSN:0161-6412

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    Objectives: Convection-enhanced delivery (CED) is a technique that delivers therapeutic agents directly and effectively into the brain parenchyma. Application of CED is now under investigation as a new treatment for various diseases. Diffuse brainstem glioma is one of the important candidates that could be targeted with CED. Especially when targeting brainstem lesions, prediction of drug distribution prior to CED will be necessary. This study evaluated the computational simulation of CED in the primate brainstem using a simplified model. Methods: Three in vivo experiments infusing gadolinium solution into the non-human primate brainstem were analyzed. T1-weighted magnetic resonance (MR) images were acquired during infusion of a total of 300 mu l gadolinium solution. Computational simulation reconstructed the surface geometry of the brainstem from the MR images. The volume of the whole structure was meshed by grid generating software. Under the assumptions that the brainstem surface was rigid and the interior was filled with cerebrospinal fluid, the equations of continuity and Darcy's law were solved within a computational fluid dynamics package using a finite volume method. The results of computational simulations were compared with those of the in vivo experiments. Results: The distribution volume (V-d) in the simulations corresponded well with the in vivo experiments. Under the condition without massive 'catheter back flow', computational simulations predicted almost 70% of the V-d of the in vivo experiments. Conclusions: The simplified computational simulations were consistent with the experiments in vivo. The methodology used in this study can be applied to predict convective drug distribution in the primate brainstem.

  109. Ultrasonic-Measurement-Integrated Simulation of Complex Blood Flow

    K. Funamoto, T. Hayase

    Proceedings of the International Workshop on Flow Dynamics Related to Energy, Aerospace and Material Science 1-2 2013/09/11

  110. In vitro study of ultrasound radiation force-driven twinkling sign using PVA-H gel and glass beads tissue-mimicking phantom. Peer-reviewed

    Lei Liu, Kenichi Funamoto, Kei Ozawa, Makoto Ohta, Toshiyuki Hayase, Masafumi Ogasawara

    Journal of medical ultrasonics (2001) 40 (3) 197-203 2013/07

    DOI: 10.1007/s10396-012-0429-9  

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    The twinkling sign observed in ultrasound coded-excitation imaging (e.g., GE B-Flow) has been reported in previous research as a potential phenomenon to detect micro calcification in soft tissue. However, the mechanism of the twinkling sign has not been clearly understood yet. We conducted an in vitro experiment to clarify the mechanism of the twinkling sign by measuring a soft tissue-mimicking phantom with ultrasonic and optical devices. A soft tissue-mimicking phantom was made of poly(vinyl alcohol) hydro (PVA-H) gel and 200-μm-diameter glass beads. We applied ultrasound to the phantom using medical ultrasound diagnostic equipment to observe the twinkling sign of glass beads. Optical imaging with a laser sheet and a high-speed camera was performed to capture the scatter lights of the glass beads with and without ultrasound radiation. The scatter lights from the glass beads were quantified and analyzed to evaluate their oscillations driven by the ultrasound radiation force. The twinkling sign from the glass beads embedded in the PVA-H gel soft tissue phantom was observed in ultrasound B-Flow color imaging. The intensity and oscillation of the scattered lights from the glass beads showed significant difference between the cases with and without ultrasound radiation. The results showed a close relationship between the occurrence of the twinkling sign and the variations of the scatter lights of glass beads, indicating that ultrasound radiation force-driven micro oscillation causes the twinkling sign of micro calcification in soft tissue.

  111. Reproduction of pressure field in ultrasonic-measurement-integrated simulation of blood flow Peer-reviewed

    Kenichi Funamoto, Toshiyuki Hayase

    INTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN BIOMEDICAL ENGINEERING 29 (7) 726-740 2013/07

    Publisher: WILEY-BLACKWELL

    DOI: 10.1002/cnm.2522  

    ISSN:2040-7939

    eISSN:2040-7947

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    Ultrasonic-measurement-integrated (UMI) simulation of blood flow is used to analyze the velocity and pressure fields by applying feedback signals of artificial body forces based on differences of Doppler velocities between ultrasonic measurement and numerical simulation. Previous studies have revealed that UMI simulation accurately reproduces the velocity field of a target blood flow, but that the reproducibility of the pressure field is not necessarily satisfactory. In the present study, the reproduction of the pressure field by UMI simulation was investigated. The effect of feedback on the pressure field was first examined by theoretical analysis, and a pressure compensation method was devised. When the divergence of the feedback force vector was not zero, it influenced the pressure field in the UMI simulation while improving the computational accuracy of the velocity field. Hence, the correct pressure was estimated by adding pressure compensation to remove the deteriorating effect of the feedback. A numerical experiment was conducted dealing with the reproduction of a synthetic three-dimensional steady flow in a thoracic aneurysm to validate results of the theoretical analysis and the proposed pressure compensation method. The ability of the UMI simulation to reproduce the pressure field deteriorated with a large feedback gain. However, by properly compensating the effects of the feedback signals on the pressure, the error in the pressure field was reduced, exhibiting improvement of the computational accuracy. It is thus concluded that the UMI simulation with pressure compensation allows for the reproduction of both velocity and pressure fields of blood flow. Copyright (c) 2012 John Wiley & Sons, Ltd.

  112. Frictional Characteristics of Erythrocytes on Endothelia-Cultured or Material-Coated Glass Plates Subject to Inclined Centrifugal Forces Invited

    Toshiyuki Hayase, Kousuke Inoue, Kenichi Funamoto, Atsushi Shirai

    Proceedings of 8th International Conference on Multiphase Flow 1-8 2013/05/29

  113. 超音波計測融合シミュレーションによる生体内の血液粘度の推定

    門脇 弘子, 船本 健一, 早瀬 敏幸, 曾根 周作, 地挽 隆夫, 橋本 浩, 見山 広二, 劉 磊

    超音波医学 40 (Suppl.) S406-S406 2013/04

    Publisher: (公社)日本超音波医学会

    ISSN:1346-1176

    eISSN:1881-9311

  114. 超音波Mモードイメージングと光電容積脈波センシングによる脈波同定の比較

    曾根 周作, 早瀬 敏幸, 船本 健一

    超音波医学 40 (Suppl.) S429-S429 2013/04

    Publisher: (公社)日本超音波医学会

    ISSN:1346-1176

    eISSN:1881-9311

  115. INFLUENCE OF PLAQUE STIFFNESS ON CHANGE OF BLOOD VESSEL GEOMETRY LEADING HEMODYNAMICAL CHANGES IN PVA-H STENOSIS MODELS Peer-reviewed

    Yasutomo Shimizu, Shuya Shida, Kenichi Funamoto, Toshiyuki Hayase, Makoto Ohta

    INTERNATIONAL MECHANICAL ENGINEERING CONGRESS AND EXPOSITION - 2012, VOL 2: BIOMEDICAL AND BIOTECHNOLOGY IMECE2012 455-460 2013

    Publisher: AMER SOC MECHANICAL ENGINEERS

    DOI: 10.1115/IMECE2012-87073  

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    One of the main factors affecting blood flow conditions in stenotic arteries is plaque geometry. Plaques can be deformed by the internal pressure, and hence plaque behavior varies depending on its stiffness. Blood flow pattern around a plaque is complicated by plaque behavior, and these complications may lead to growth of the plaque itself. Thus, we can say that geometry and mechanical properties of a plaque, and blood flow will affect each other. To understand the relationship between plaque stiffness and flow pattern, flow measurement using elastic models, which mimic the mechanical properties of blood vessels, is required. Flow patterns with steady flows and a range of hydrostatic pressures were observed by particle image velocimetry. The results show that the model is deformed by hydrostatic pressures. Furthermore, flow patterns are also changed as the results of model deformation, especially at reattachment points. Simultaneously, we performed a numerical simulation for finding a critical parameter of the flow patterns. These results show that the reattachment length increases in the model with high stenosis severity and in a vertically oriented parent artery. In conclusion, a parent artery and plaque can deform because of internal pressure, and these deformation will affect blood flow patterns.

  116. Fundamental Study on Micro Calcification Detection Using Twinkling Sign (TS): The Effect of Stiffness of Surrounding Tissue on the Appearance of TS Peer-reviewed

    Lei Liu, Kenichi Funamoto, Masayuki Tanabe, Toshiyuki Hayase

    2013 35TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) 1390-1393 2013

    Publisher: IEEE

    DOI: 10.1109/EMBC.2013.6609769  

    ISSN:1557-170X

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    The twinkling sign (TS) observed in ultrasound imaging (e. g., color flow mode and pulse Doppler mode) has been reported in previous researches as a potential phenomenon to detect micro calcification in soft tissue. However, the mechanism of the twinkling sign has not been clearly understood yet. The authors investigated the effect of stiffness of surrounding tissue on the appearance of TS using the soft tissue-mimicking phantoms and a medical ultrasound device. The author used Poly (vinyl alcohol) hydro (PVA-H) gel as the material of phantom and developed three phantoms with different PVA concentration; 8 % wt, 10 % wt and 15 % wt those correspond to Young's modulus (E) as 50 kPa, 100 kPa and 230 kPa, respectively. Micro glass and CaCO3 particles were embedded in the phantoms as pseudo micro calcification. The authors observed TS in each phantom and analyzed the temporal average of TS. The temporal average of TS was largest in the 8 % wt (E = 50kPa) PVA-H gel phantom, and decreased with increasing the phantom stiffness. The result indicated that the micro oscillation of the particles had a close relationship with the occurrence of TS.

  117. Simultaneous Analysis System for Blood Pressure and Flow Using Photoplethysmography and Ultrasonic-Measurement-Integrated Simulation Peer-reviewed

    Shusaku Sone, Toshiyuki Hayase, Kenichi Funamoto, Atsushi Shirai

    2013 35TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) 1827-1830 2013

    Publisher: IEEE

    DOI: 10.1109/EMBC.2013.6609878  

    ISSN:1557-170X

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    We developed a simultaneous analysis system for blood pressure and flow using photoplethysmography and ultrasonic-measurement-integrated simulation. The validity of the system was confirmed by analysis of blood flow field in a carotid artery and corresponding wave intensity (WI) values.

  118. Erratum to "Influence of surface model extraction parameter on computational fluid dynamics modeling of cerebral aneurysms" [Journal of Biomechanics 45 (2012) 2355-2361] Peer-reviewed

    Shunsuke Omodaka, Takashi Inoue, Kenichi Funamoto, Shin-ichirou Sugiyama, Hiroaki Shimizu, Toshiyuki Hayase, Akira Takahashi, Teiji Tominaga

    Journal of Biomechanics 45 (16) 2947 2012/11/15

    DOI: 10.1016/j.jbiomech.2012.09.012  

    ISSN:0021-9290 1873-2380

  119. A novel microfluidic platform for high-resolution imaging of a three-dimensional cell culture under a controlled hypoxic environment Peer-reviewed

    Kenichi Funamoto, Ioannis K. Zervantonakis, Yuchun Liu, Christopher J. Ochs, Choong Kim, Roger D. Kamm

    LAB ON A CHIP 12 (22) 4855-4863 2012/11

    Publisher: ROYAL SOC CHEMISTRY

    DOI: 10.1039/c2lc40306d  

    ISSN:1473-0197

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    Low oxygen tensions experienced in various pathological and physiological conditions are a major stimulus for angiogenesis. Hypoxic conditions play a critical role in regulating cellular behaviour including migration, proliferation and differentiation. This study introduces the use of a microfluidic device that allows for the control of oxygen tension for the study of different three-dimensional (3D) cell cultures for various applications. The device has a central 3D gel region acting as an external cellular matrix, flanked by media channels. On each side, there is a peripheral gas channel through which suitable gas mixtures are supplied to establish a uniform oxygen tension or gradient within the device. The effects of various parameters, such as gas and media flow rates, device thickness, and diffusion coefficients of oxygen were examined using numerical simulations to determine the characteristics of the microfluidic device. A polycarbonate (PC) film with a low oxygen diffusion coefficient was embedded in the device in proximity above the channels to prevent oxygen diffusion from the incubator environment into the polydimethylsiloxane (PDMS) device. The oxygen tension in the device was then validated experimentally using a ruthenium-coated (Ru-coated) oxygen-sensing glass cover slip which confirmed the establishment of low uniform oxygen tensions (&lt;3%) or an oxygen gradient across the gel region. To demonstrate the utility of the microfluidic device for cellular experiments under hypoxic conditions, migratory studies of MDA-MB-231 human breast cancer cells were performed. The microfluidic device allowed for imaging cellular migration with high-resolution, exhibiting an enhanced migration in hypoxia in comparison to normoxia. This microfluidic device presents itself as a promising platform for the investigation of cellular behaviour in a 3D gel scaffold under varying hypoxic conditions.

  120. Hemodynamic Analysis of Growing Intracranial Aneurysms Arising from a Posterior Inferior Cerebellar Artery Peer-reviewed

    Shin-Ichiro Sugiyama, Hui Meng, Kenichi Funamoto, Takashi Inoue, Miki Fujimura, Toshio Nakayama, Shunsuke Omodaka, Hiroaki Shimizu, Akira Takahashi, Teiji Tominaga

    WORLD NEUROSURGERY 78 (5) 462-468 2012/11

    Publisher: ELSEVIER SCIENCE INC

    DOI: 10.1016/j.wneu.2011.09.023  

    ISSN:1878-8750

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    OBJECTIVE: The role of hemodynamics in the growth of intracranial aneurysms is not completely clear. We present a hemodynamic study with two adjacent unruptured aneurysms arising from one parent artery but growing in different ways. This study aimed to investigate whether there were differences in hemodynamic characteristics between the two growing aneurysms. METHODS: A 62-year-old female patient presented with six unruptured intracranial aneurysms. Catheter angiography at 6-month intervals revealed that two aneurysms located adjacently at the right posterior inferior cerebellar artery were growing over a 1-year period. Three-dimensional aneurysm geometries were acquired via rotational angiography. Computational fluid dynamic simulations were conducted on the 3D aneurysm geometries under patient-specific pulsatile flow conditions that were measured by magnetic resonance velocimetry. RESULTS: The proximal multilobular aneurysm demonstrated high flow and physiological levels of wall shear stress (WSS) in the region of growth, whereas the distal rounded aneurysm had low flow and low WSS in the growing sac. CONCLUSION: Growing aneurysms can have heterogeneous hemodynamic and morphologic characteristics and different growing patterns. Growing regions of an aneurysm could be exposed to either high WSS at the inflow zone or low WSS and high oscillatory shear in the aneurysm sac.

  121. Numerical Analysis of Levitation Mechanism of Red Blood Cell in Inclined Centrifuge Microscope - Effect of Asymmetric Cell Shape on the Motion

    Takashi OSHIBE, Toshiyuki HAYASE, Kenichi FUNAMOTO, Atsushi SHIRAI

    Proceedings of the 9th International Conference on Flow Dynamics (ICFD2012) 756-757 2012/09/20

  122. Rheological Analysis of the Mechanism of Fetal Brain Hemorrhage

    T. Ito, K. Funamoto, K. Funamoto, T. Hayase, Y. Kimura

    Proceedings of the 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 116-117 2012/09/20

  123. Computational Simulation to Create Low Oxygen Tension in a Microfluidic Device for Cell Culture

    K. Funamoto, I.K. Zervantonakis, Y. Liu, C.J. Ochs, R.D. Kamm

    Proceedings of the 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 88-89 2012/09/20

  124. Ultrasound Radiation Force Driven B-Flow Twinkling Sign for Microcalcification Detection

    L. Liu, K. Funamoto, K. Ozawa, M. Ohta, T. Hayase, M. Ogasawara

    Proceedings of the 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 86-87 2012/09/20

  125. Observation of Hypoxia Cellular Response by Using Microfluidic Devices

    S. Fukushima, R. Maehara, K. Funamoto

    Proceedings of the 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 122-123 2012/09/20

  126. Magnetic Resonance Fluid Dynamics for Intracranial Aneurysms

    S. Sugiyama, K. Funamoto, T. Hayase, T. Tominaga

    Proceedings of the 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 118-119 2012/09/20

  127. Oxygen Tension Control in a Microfluidic Device for Cell Culture Invited

    K. Funamoto, I.K. Zervantonakis, Y. Liu, R.D. Kamm

    Proceedings of the 9th International Conference on Flow Dynamics (ICFD2012) 724-725 2012/09/19

  128. Intra-left Atrial Flow

    M. Shibata, T. Yambe, K. Funamoto, T. Hayase

    Proceedings of the 9th International Conference on Flow Dynamics (ICFD2012) 404-405 2012/09/19

  129. Influence of surface model extraction parameter on computational fluid dynamics modeling of cerebral aneurysms Peer-reviewed

    Shunsuke Omodaka, Takashi Inoue, Kenichi Funamoto, Shin-ichirou Sugiyama, Hiroaki Shimizu, Toshiyuki Hayase, Akira Takahashi, Teiji Tominaga

    JOURNAL OF BIOMECHANICS 45 (14) 2355-2361 2012/09

    Publisher: ELSEVIER SCI LTD

    DOI: 10.1016/j.jbiomech.2012.07.006  

    ISSN:0021-9290

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    Threshold image intensity for reconstructing patient-specific vascular models is generally determined subjectively. We assessed the effects of threshold image intensity differences on computational fluid dynamics (CFD) using a simple method of threshold determination. This study included 11 consecutive patients with internal carotid artery aneurysms collected retrospectively between April 2009 and March 2010. In 3-dimensional rotational angiography image data, we set a line probe across the coronal cross-section of the parent internal carotid artery, and calculated a profile curve of the image intensity along this line. We employed the threshold coefficient (C-thre) value in this profile curve, in order to determine the threshold image intensity objectively. We assessed the effects of C-thre value differences on vascular model configuration and the wall shear stress (WSS) distribution of the aneurysm. The threshold image intensity increased as the C-thre value increased. The frequency of manual editing increased as the C-thre value decreased, while disconnection of the posterior communicating artery occurred more frequently as the C-thre value increased. The volume of the vascular model decreased and WSS increased according to the C-thre value increase. The pattern of WSS distribution changed remarkably in one case. Threshold image intensity differences can produce profound effects on CFD. Our results suggest the uniform setting of C-thre value is important for objective CFD. (c) 2012 Elsevier Ltd. All rights reserved.

  130. Neumerical and Experimental Study of Flow in Stenosis models with Several Mechanical Properties

    Yasutomo Shimizu, Shuya Shida, Ashkan Javadzadegan, Kenichi Funamoto, Toshiyuki Hayase, Makoto Ohta

    Proceedings Ninth International Conference on Flow Dynamics 422-423 2012/09

  131. Oxygen sensors for microfluidic 3D cell cultures

    C.J. Ochs, K. Funamoto, R.D. Kamm, D. Trau

    EUROPT(R)ODE XI Conference on Optical Chemical Sensors and Biosensors 98 2012/04/02

  132. 2次元超音波計測融合シミュレーションによる頸動脈分岐部の非定常血流量推定

    門脇 弘子, 船本 健一, 早瀬 敏幸, 曾根 周作, 小笠原 正文, 地挽 隆夫, 橋本 浩, 見山 広二

    超音波医学 39 (Suppl.) S338-S338 2012/04

    Publisher: (公社)日本超音波医学会

    ISSN:1346-1176

    eISSN:1881-9311

  133. Local Hemodynamics at the Rupture Point of Cerebral Aneurysms Determined by Computational Fluid Dynamics Analysis Peer-reviewed

    Shunsuke Omodaka, Shin-ichirou Sugiyama, Takashi Inoue, Kenichi Funamoto, Miki Fujimura, Hiroaki Shimizu, Toshiyuki Hayase, Akira Takahashi, Teiji Tominaga

    CEREBROVASCULAR DISEASES 34 (2) 121-129 2012

    Publisher: KARGER

    DOI: 10.1159/000339678  

    ISSN:1015-9770

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    Background: Cerebral aneurysms carry a high risk of rupture and so present a major threat to the patient's life. Accurate criteria for predicting aneurysm rupture are important for therapeutic decision-making, and some clinical and morphological factors may help to predict the risk for rupture of unruptured aneurysms, such as sex, size and location. Hemodynamic forces are considered to be key in the natural history of cerebral aneurysms, but the effect on aneurysm rupture is uncertain, and whether low or high wall shear stress (WSS) is the most critical in promoting rupture remains extremely controversial. This study investigated the local hemodynamic features at the aneurysm rupture point. Methods: Computational models of 6 ruptured middle cerebral artery aneurysms with intraoperative confirmation of rupture point were constructed from 3-dimensional rotational angiography images. Computational fluid dynamics (CFD) simulations were performed under pulsatile flows using patient-specific inlet flow conditions. Time-averaged WSS (TAWSS) and oscillatory shear index (OSI) were calculated, and compared at the rupture point and at the aneurysm wall without the rupture point. We performed an additional CFD simulation of a bleb-removed model for a peculiar case in which bleb formation could be confirmed by magnetic resonance angiography. Results: All rupture points were located at the body or dome of the aneurysm. The TAWSS at the rupture point was significantly lower than that at the aneurysm wall without the rupture point (1.10 vs. 4.96 Pa, p = 0.031). The OSI at the rupture point tended to be higher than at the aneurysm wall without the rupture point, although the difference was not significant (0.0148 vs. 0.0059, p = 0.156). In a bleb-removed simulation, the TAWSS at the bleb-removed area was 6.31 Pa, which was relatively higher than at the aneurysm wall (1.94 Pa). Conclusion: The hemodynamics of 6 ruptured cerebral aneurysms of the middle cerebral artery were examined using retrospective CFD analysis. We could confirm the rupture points in all cases. With those findings, local hemodynamics of ruptured aneurysms were quantitatively investigated. The rupture point is located in a low WSS region of the aneurysm wall. Bleb-removed simulation showed increased WSS of the bleb-removed area, associated with the flow impaction area. Although the number of subjects in this study was relatively small, our findings suggest that the location of the rupture point is related to a low WSS at the aneurysm wall. Further investigations will elucidate the detailed hemodynamic effects on aneurysm rupture. Copyright (c) 2012 S. Karger AG, Basel

  134. Flow Analysis of Rupture Point in Cerebral Aneurysms: Computational Fluid Dynamics Study

    S. Omodaka, T. Inoue, K. Funamoto, S. Sugiyama, M. Fujimura, H. Shimizu, T. Hayase, A. Takahashi, T. Tominaga

    Proceedings on the 5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering 68-69 2011/12/13

  135. Numerical Analysis of the Effect of Angle of Attack on a Red Blood Cell Moving in an Inclined Centrifuge Microscope

    T. Oshibe, T. Hayase, K. Funamoto, A. Shirai

    Proceedings on the 5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering 82-83 2011/12/13

  136. Two-Dimensional Ultrasonic-Measurement-Integrated Simulation of Blood Flow in Bifurcation of Carotid Artery

    H. Kadowaki, K. Funamoto, T. Hayase, S. Sone, M. Ogasawara, T. Jibiki, H. Hashimoto, K. Miyama

    Proceedings on the 5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering 80-81 2011/12/13

  137. Implementation of visual data mining for unsteady blood flow field in an aortic aneurysm Peer-reviewed

    Seiichiro Morizawa, Koji Shimoyama, Shigeru Obayashi, Kenichi Funamoto, Toshiyuki Hayase

    JOURNAL OF VISUALIZATION 14 (4) 393-398 2011/12

    Publisher: SPRINGER

    DOI: 10.1007/s12650-011-0101-2  

    ISSN:1343-8875

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    This study was performed to determine the relations between the features of wall shear stress and aneurysm rupture. For this purpose, visual data mining was performed in unsteady blood flow simulation data for an aortic aneurysm. The time-series data of wall shear stress given at each grid point were converted to spatial and temporal indices, and the grid points were sorted using a self-organizing map based on the similarity of these indices. Next, the results of cluster analysis were mapped onto the real space of the aortic aneurysm to specify the regions that may lead to aneurysm rupture. With reference to previous reports regarding aneurysm rupture, the visual data mining suggested specific hemodynamic features that cause aneurysm rupture.

  138. Comparison between Ultrasonic-Measurement-Integrated Simulation and Ordinary Simulation with Measured Upstream Velocity Condition

    S. Sone, T. Kato, K. Funamoto, T. Hayase, M. Ogasawara, T. Jibiki, H. Hashimoto, K. Miyama

    Proceedings of the 8th International Conference on Flow Dynamics 456-457 2011/11/10

  139. Computational Simulation of Blood Flow in Intracranial Aneurysms under Patient-Specific Pulsatile Inlet Condition

    S. Sugiyama, K. Funamoto, T. Hayase, T. Tominaga

    Proceedings of the 11th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2011) 90-91 2011/11/10

  140. Detection of Microcalcification in Soft Tissue Employing B-Flow ”Twinkling” Sign

    L. Liu, K. Ozawa, K. Funamoto, M. Ohta, T. Hayase, M. Ogasawara

    Proceedings of the 11th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2011) 94-95 2011/11/10

  141. Left Atrial Vortex

    M. Shibata, T. Yambe, K. Funamoto, T. Hayase

    Proceedings of the 11th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2011) 88-89 2011/11/10

  142. Rheological Analysis of the Mechanism of Fetal Brain Hemorrhage

    T. Ito, K. Funamoto, K. Funamoto, K. Tanabe, A. Nakamura, T. Hayase, Y. Kimura

    Proceedings of the 11th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2011) 86-87 2011/11/10

  143. Volumetric and Angiogenic Evaluation of Antitumor Effects with Acoustic Liposome and High-Frequency Ultrasound Peer-reviewed

    Tetsuya Kodama, Noriko Tomita, Yoko Yagishita, Sachiko Horie, Kenichi Funamoto, Toshiyuki Hayase, Maya Sakamoto, Shiro Mori

    CANCER RESEARCH 71 (22) 6957-6964 2011/11

    Publisher: AMER ASSOC CANCER RESEARCH

    DOI: 10.1158/0008-5472.CAN-11-2389  

    ISSN:0008-5472

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    Acoustic liposomes (AL) have their inherent echogenicity and can add functionality in serving as drug carriers with tissue specificity. Nonuniform vascular structures and vascular branches/bends are evaluated by imaging the intravascular movement locus of ALs with high-frequency ultrasound (HF-US) imaging. However, the evaluation of antitumor effects on angiogenesis by ALs and HF-US imaging has not been reported. Here, we show that the combination of ALs and an HF-US imaging system is capable of noninvasively evaluating antitumor volumetric and angiogenic effects in preclinical mouse models of various cancers. In this study, the antitumor effects of cisplatin on tumor growth and angiogenesis in mice bearing two different types of tumor cells were assessed. By tracking each AL flowing in the vessel and transferring the images to personal computers, microvessel structures were mapped and reconstructed using the color difference based on SD method. The antitumor effects were confirmed with an in vivo bioluminescence imaging system and immunohistochemical analysis. Our results show that cisplatin inhibits tumor growth by decreasing intratumoral vessel area but does not affect the angiogenesis ratio in the tumor. The vascular occupancy in the outer region of the tumor was larger than that in the inner region; however, both occupancies were similar to those of the control tumor. We propose that this method of mapping microvessels with ALs and an HF-US system can serve as a new molecular imaging method for the assessment of angiogenesis and can be applied to evaluate the antitumor effects by various therapeutic agents. Cancer Res; 71(22); 6957-64. (C)2011 AACR.

  144. 超音波計測融合シミュレーションと計測による上流速度を与えたシミュレーションの比較

    曾根 周作, 加藤 宇海, 船本 健一, 早瀬 敏幸, 小笠原 正文, 地挽 隆夫, 橋本 浩, 見山 広二

    超音波医学 38 (Suppl.) S326-S326 2011/04

    Publisher: (公社)日本超音波医学会

    ISSN:1346-1176

    eISSN:1881-9311

  145. Integration of Ultrasound Color Doppler Imaging and Numerical Simulation

    K. Funamoto, T. Kato, T. Hayase

    Proceedings of the 2nd International Conference on Computational and Mathematical Biomedical Engineering (CMBE11) 262-265 2011/04/01

  146. Numerical Analysis of Effects of Measurement Errors on Ultrasonic-Measurement-Integrated Simulation Peer-reviewed

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING 58 (3) 653-663 2011/03

    Publisher: IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC

    DOI: 10.1109/TBME.2010.2095418  

    ISSN:0018-9294

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    Ultrasonic-measurement-integrated (UMI) simulation, in which feedback signals are applied to the governing equations based on errors between ultrasonic measurement and numerical simulation, has been investigated for reproduction of the blood flow field. However, ultrasonic measurement data inherently include some errors. In this study, the effects of four major measurement errors, namely, errors due to Gaussian noise, aliasing, wall filter, and lack of data, on UMI simulation were examined by a numerical experiment dealing with the blood flow field in the descending aorta with an aneurysm, the same as in our previous study. While solving the governing equations in UMI simulation, Gaussian noise did not prevent the UMI simulation from effectively reproducing the blood flow field. In contrast, aliasing caused significant errors in UMI simulation. Effects of wall filter and lack of data appeared in diastole and in the whole period, respectively. By detecting significantly large feedback signals as a sign of aliasing and by not adding feedback signals where measured Doppler velocities were aliasing or zero, the computational accuracy substantially improved, alleviating the effects of measurement errors. Through these considerations, UMI simulation can provide accurate and detailed information on hemodynamics with suppression of four major measurement errors.

  147. Hemodynamic changes in the left atrium due to atrial fibrillation

    Kenichi Funamoto, Ryo Koizumi, Toshiyuki Hayase, Muneichi Shibata, Tomoyuki Yambe

    ASME 2011 Summer Bioengineering Conference, SBC 2011 1131-1132 2011

    DOI: 10.1115/SBC2011-53817  

  148. Blood Flow Analysis in the Left Atrium with/without Atrial Fibrillation

    R. Koizumi, K. Funamoto, T. Hayase, M. Shibata

    Proceedings on the 4th East Asian Pacific Student Workshop on Nano-Biomedical Engineering 96-97 2010/12/16

  149. Experimental Validation of Ultrasonic-Measurement-Integrated Blood Flow Simulation Using Carotid Artery Models

    T. Sawao, K. Funamoto, T. Hayase

    Proceedings on the 4th East Asian Pacific Student Workshop on Nano-Biomedical Engineering 158-159 2010/12/15

  150. Stabilization of Measurement-Integrated Simulation by Elucidation of Destabilizing Mechanism Peer-reviewed

    Toshiyuki Hayase, Kentaro Imagawa, Kenichi Funamoto, Atsushi Shirai

    Journal of Fluid Science and Technology 5 (3) 632-647 2010/12

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jfst.5.632  

    ISSN:1880-5558

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    Measurement-integrated (MI) simulation is a numerical flow analysis method with a feedback mechanism from measurement of a real flow. It correctly reproduces a real flow under inherent ambiguity in a mathematical model or a computational condition. In this paper we theoretically investigated the destabilization phenomenon of MI simulation, in which analysis error suddenly increases at some critical feedback gain. This phenomenon has been considered as instability of a closed-loop feedback system, but present study treated it as that of a numerical scheme. First, the mechanism of the destabilization phenomenon was investigated based on the sufficient condition of the convergence of iterative calculation of existing MI simulation. It was found that the feedback signal in the source term destabilized the iterative calculation. Then, a new MI simulation scheme was derived by evaluating the feedback signal in the linear term to remove the cause of the destabilization. The validity of the present theoretical analysis was verified for examples treated in former studies of MI simulations: blood flow in an aneurismal aorta with ultrasonic measurement, blood flow in a cerebral aneurism with magnetic resonance measurement, Karman vortex street behind a square cylinder with PIV measurement, and fully developed turbulent flow in a square duct with ideal measurement. Occurrences of destabilization phenomenon in all the examples were well explained by the condition of this study, especially for cases of relatively small time steps and large feedback gains. Furthermore, the new MI simulation scheme realized the analysis without the destabilization phenomenon. The present theoretical result confirming that the destabilization phenomenon is not the instability of the feedback system but that of a numerical scheme is generally applicable to MI simulations using the velocity error for the feedback signal.

  151. Computer Simulation Predicts the Convective Drug Distribution in the Primate Brainstem

    S. Sugiyama, R. Saito, K. Funamoto, Y. Sonoda, T. Kumabe, T. Hayase, T. Tominaga

    Proceedings of the tenth International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2010) 98-99 2010/11/02

  152. Consideration for Simulations of Infusion in Realistic Animal Brain Geometries

    J. H. Smith, K. Funamoto, K. A. Starkweather, T. Hayase

    Proceedings of the tenth International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2010) 96-97 2010/11/02

  153. Detection of Microcalcification in Soft Tissue

    L. Liu, M. Ogasawara, K. Ozawa, K. Funamoto, M. Ohta, T. Hayase

    Proceedings of the tenth International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2010) 80-81 2010/11/02

  154. Evaluation of Wall Shear Stress on Carotid Artery with Ultrasonic-Measurement-Integrated Simulation

    T. Kato, K. Funamoto, T. Hayase, M. Ogasawara, T. Jibiki, H. Hashimoto, K. Miyama

    Proceedings of the seventh International Conference on Flow Dynamics 542-543 2010/11/02

  155. Fluid Analysis of the Mechanism of Fetal Brain Hemorrhage

    T. Ito, K. Funamoto, K. Funamoto, K. Tanabe, A. Nakamura, T. Hayase, Y. Kimura

    Proceedings of the tenth International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2010) 78-79 2010/11/02

  156. Left Atrial Appendage

    M. Shibata, T. Yambe, T. Yamaguchi, R. Koizumi, K. Funamoto, T. Hayase

    Proceedings of the tenth International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2010) 94-95 2010/11/02

  157. Computational Simulation on Convection-Enhanced Drug Delivery into the Primate Brain

    S. Sugiyama, T. Nakayama, K. Funamoto, T. Hayase, T. Tominaga

    Abstracts of the 6th World Congress of Biomechanics 578-579 2010/08/04

  158. Determination of Local Fine Structure of Blood Flows by Measurement Coupled Simulation Invited

    T. Hayase, K. Funamoto, K. Imagawa

    Proceedings of 12th International Symposium of Tohoku University Global COE Programme Global Nano-Biomedical Engineering Education and Research Network Centre 89-92 2010/03

  159. Clinical Application of Ultrasonic-Measurement-Integrated Simulation for Diagnosis of Carotid Artery

    K. Funamoto, T. Kato, T. Hayase, M. Ogasawara, T. Jibiki, H. Hashimoto, K. Miyama

    Proceedings of SMART-Tohoku GCOE joint Workshop on Micro & Nano Bioengineering: MIT, NUS, NTU and Tohoku 41-42 2010/01/12

  160. Visual Data Mining for Unsteady Blood Flow Field, Peer-reviewed

    S. Morizawa, K. Shimoyama, S. Obayashi, K. Funamoto, T. Hayase

    OE-001 2010

  161. VISUALIZATION OF MICROCIRCULATION BASED ON BRIGHTNESS VARIATION IN CONTRAST-ENHANCED ULTRASOUND

    Kenichi Funamoto, Toshiyuki Hayase, Tetsuya Kodama

    PROCEEDINGS OF THE ASME SUMMER BIOENGINEERING CONFERENCE, 2010 219-220 2010

    Publisher: AMER SOC MECHANICAL ENGINEERS

    DOI: 10.1115/SBC2010-19349  

  162. Effect of Measurement Error on Ultrasonic-Measurement-Integrated Simulation of Blood Flow in an Aortic Aneurysm

    K. Funamoto, T. Hayase, Y. Saijo, T. Yambe

    6TH WORLD CONGRESS OF BIOMECHANICS (WCB 2010), PTS 1-3 31 1652-+ 2010

    Publisher: SPRINGER

    ISSN:1680-0737

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    Stresses due to blood flow on a blood vessel wall (hemodynamic stresses) are closely related to development and progression of circulatory diseases such as atherosclerosis and aneurysm. Therefore, for advanced diagnosis of circulatory diseases, accurate and detailed information of hemodynamics is necessary. To reproduce blood flow field, we have proposed ultrasonic-measurement-integrated (UMI) simulation, in which feedback signals are applied to the governing equations based on errors between ultrasonic measurement and numerical simulation at feedback points. Efficiency of the UMI simulation was shown by our previous numerical experiment dealing with a three-dimensional unsteady blood flow field in the descending aorta with an aneurysm. However, real ultrasonic measurement data inherently includes some errors. In this study, the effects of four major measurement errors, namely, errors due to Gaussian noise, aliasing, wall filter and lack of data, on computational accuracy of the UMI simulation were examined by a numerical experiment dealing with the blood flow field in an aortic aneurysm, the same as in our previous study. While solving the governing equations in UMI simulation, Gaussian noise did not work as an effective feedback signal, and, therefore, hardly influenced the computational result. In contrast, aliasing caused significant errors in the UMI simulation. By detecting significantly large feedback signals as a sign of aliasing and by replacing the measured Doppler velocity with the computational one, the computational accuracy of the UMI simulation was substantially improved. Effects of wall filter and lack of data especially appeared in diastole and in systole, respectively, but they were alleviated by not adding feedback signals where measured Doppler velocities were zero. Hence, UMI simulation can be performed with suppression of measurement errors.

  163. Analysis of Clinical Data with Ultrasonic-Measurement-Integrated Simulation

    T. Kato, K. Funamoto, T. Hayase, M. Ogasawara, T. Jibiki, H. Hashimoto, K. Miyama

    Proceedings of the 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering 102-103 2009/12

  164. Dynamic Characteristics Analysis of Diseased Circulatory System with Lumped Parameter Model 1st Report : Heart Valve Disease

    R. Koizumi, T. Hayase, K. Funamoto

    Proceedings of the 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering 6-7 2009/12

  165. Eigenvalue Analysis for Error Dynamics of Ultrasonic-Measurement-Integrated Simulation of Blood Flow in the Aneurismal Aorta

    K. Imagawa, T. Hayase, K. Funamoto

    Proceedings of the 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering 114-115 2009/12

  166. Three-Dimensional Ultrasonic-Measurement-Integrated Blood flow Simulation using PVA Carotid Artery Model

    T. Suzuki, T. Hayase, K. Funamoto, K. Inoue

    Proceedings of the 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering 116-117 2009/12

  167. Atrial Vortex

    M. Shibata, H. Ito, T. Yambe, R. Koizumi, K. Funamoto, T. Hayase

    Proceedings of the 9th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration 64-65 2009/11/05

  168. Blood Flow Analysis by Measurement-Integrated Simulation

    K. Funamoto, T. Hayase

    Proceedings of the 9th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration 120-121 2009/11/05

  169. Convection-enhanced Delivery of ACNU under MRI Monitoring against Recurrent Gliomas-Development of Computational Simulation of Drug Distribution

    R. Saito, Y. Sonoda, T. Kumabe, K. Funamoto, T. Hayase, T. Tominaga

    Proceedings of the 9th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration 72-73 2009/11/05

  170. Fundamental Study of Convection-Enhanced Delivery Simulation in Rat Brain

    J.H. Smith, K. Funamoto, M.V. Racenis, T. Hayase

    Proceedings of the 9th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration 70-71 2009/11/05

  171. Numerical Validation of MR-Measurement-Integrated Simulation of Blood Flow in a Cerebral Aneurysm Peer-reviewed

    Kenichi Funamoto, Yoshitsugu Suzuki, Toshiyuki Hayase, Takashi Kosugi, Haruo Isoda

    ANNALS OF BIOMEDICAL ENGINEERING 37 (6) 1105-1116 2009/06

    Publisher: SPRINGER

    DOI: 10.1007/s10439-009-9689-y  

    ISSN:0090-6964

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    This study proposes magnetic resonance (MR)-measurement-integrated (MR-MI) simulation, in which the difference between the computed velocity field and the phase-contrast MRI measurement data is fed back to the numerical simulation. The computational accuracy and the fundamental characteristics, such as steady characteristics and transient characteristics, of the MR-MI simulation were investigated by a numerical experiment. We dealt with reproduction of three-dimensional steady and unsteady blood flow fields in a realistic cerebral aneurysm developed at a bifurcation. The MR-MI simulation reduced the error derived from the incorrect boundary conditions in the blood flow in the cerebral aneurysm. For the reproduction of steady and unsteady standard solutions, the error of velocity decreased to 13% and to 22% in one cardiac cycle, respectively, compared with the ordinary simulation without feedback. Moreover, the application of feedback shortened the computational convergence, and thus the convergent solution and periodic solution were obtained within less computational time in the MR-MI simulation than that in the ordinary simulation. The dividing flow ratio toward the two outlets after bifurcation was well estimated owing to the improvement of computational accuracy. Furthermore, the MR-MI simulation yielded wall shear stress distribution on the cerebral aneurysm of the standard solution accurately and in detail.

  172. Effect of Aliasing on Ultrasonic-Measurement-Integrated Simulation of Three-Dimensional Unsteady Blood Flow

    K. Funamoto, T. Hayase, K. Imagawa, Y. Saijo, T. Yambe

    Proceedings of the 10th International Symposium of 2007 Tohoku University Global COE Program “Global Nano-Biomedical Engineering Education and Research Network Centre” 18-19 2009/04/17

  173. Fluid-Structure Coupled Ultrasonic-Measurement-Integrated Simulation of Fluid in Elastic Tube with Contraction

    L. Liu, T. Hayase, K. Funamoto

    Proceedings of 9th International Symposium of Tohoku University Global COE Programme Global Nano-Biomedical Engineering Education and Research Network Centre 106-107 2009/03

  174. NUMERICAL EXPERIMENT OF MR-MEASUREMENT-INTEGRATED SIMULATION OF STEADY BLOOD FLOW IN A CEREBRAL ANEURYSM

    Kenichi Funamoto, Yoshitsugu Suzuki, Toshiyuki Hayase, Takashi Kosugi, Haruo Isoda

    PROCEEDINGS OF THE ASME SUMMER BIOENGINEERING CONFERENCE 2008, PTS A AND B 1089-1090 2009

    Publisher: AMER SOC MECHANICAL ENGINEERS

  175. Numerical Evaluation of MR-Measurement-Integrated Simulation of Unsteady Hemodynamics in a Cerebral Aneurysm

    K. Funamoto, Y. Suzuki, T. Hayase, T. Kosugi, H. Isoda

    13TH INTERNATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING, VOLS 1-3 23 (1-3) 2188-+ 2009

    Publisher: SPRINGER

    ISSN:1680-0737

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    Detailed and accurate information of hemodynamics is essential for clarification and advanced diagnosis of circulatory diseases. In order to reproduce complicated real flow field, the concept of flow observer, which integrates measurement and computation by feedback process, have been proposed. By applying the method to the MR (Magnetic Resonance) measurement, we propose MR-measurement-integrated (MR-MI) simulation: the difference between the Phase-contrast MRI (PC MRI) data and the computational result of three-dimensional velocity vector field is fed back to the numerical simulation. The objective in this study is to evaluate the MR-MI simulation by numerical experiment dealing with an unsteady blood flow in a cerebral aneurysm. In the numerical experiment, we first defined a standard solution with velocity profiles obtained by PC MRI measurement at boundaries as a model of real blood flow. Then, MR-MI simulation was carried out by assuming a uniform velocity profile at the upstream boundary and pressure zero and free flow conditions at the downstream boundaries. During the computation, a feedback signal being proportional to the difference between the standard solution and the computational result was applied at each grid point in the aneurysm. As the result, the MR-MI simulation reproduced the standard solution in the aneurysm owing to the feedback process. The error derived from the inaccurate boundary conditions decreased to 22% in one cardiac cycle, comparing to the ordinary simulation. Consequently, the MR-MI simulation accurately provided the wall shear stress distribution on the cerebral aneurysm as hemodynamic information.

  176. Numerical Experiment of Transient and Steady Characteristics of Ultrasonic-Measurement-Integrated Simulation in Three-Dimensional Blood Flow Analysis Peer-reviewed

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    ANNALS OF BIOMEDICAL ENGINEERING 37 (1) 34-49 2009/01

    Publisher: SPRINGER

    DOI: 10.1007/s10439-008-9600-2  

    ISSN:0090-6964

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    In ultrasonic-measurement-integrated (UMI) simulation of blood flows, feedback signals proportional to the difference of velocity vector optimally estimated from Doppler velocities are applied in the feedback domain to reproduce the flow field. In this paper, we investigated the transient and steady characteristics of UMI simulation by numerical experiment. A steady standard numerical solution of a three-dimensional blood flow in an aneurysmal aorta was first defined with realistic boundary conditions. The UMI simulation was performed assuming that the realistic velocity profiles in the upstream and downstream boundaries were unknown but that the Doppler velocities of the standard solution were available in the aneurysmal domain or the feedback domain by virtual color Doppler imaging. The application of feedback in UMI simulation resulted in a computational result approach to the standard solution. As feedback gain increased, the error decreased faster and the steady error became smaller, implying the traceability to the standard solution improves. The positioning of ultrasound probes influenced the result. The height less than or equal to the aneurysm seemed better choice for UMI simulation using one probe. Increasing the velocity information by using multiple probes enhanced the UMI simulation by achieving ten times faster convergence and more reduction of error.

  177. REPRODUCTION OF BLOOD FLOW FIELD BY NUMERICAL SIMULATION INTEGRATED WITH ULTRASONIC MEASUREMENT WITH NOISE

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    PROCEEDINGS OF THE ASME SUMMER BIOENGINEERING CONFERENCE - 2009, PT A AND B 455-456 2009

    Publisher: AMER SOC MECHANICAL ENGINEERS

    DOI: 10.1115/SBC2009-206596  

  178. Development of Poly (Vinyl Alchol) Gel with in vivo Acoustic Properties

    Osamu Yamashita, Kenichi Funamoto, Toshiyuki Hayase

    Proceedings of GPBE/NUS-Tohoku Graduate Student Conference in Bioengineering 30-31 2008/12

  179. Physics Data Mining for Three-Dimensional Unsteady Blood Flow Field in an Aneurysmal Aorta

    Shinichiro Morizawa, Koji Shimoyama, Shigeru Obayashi, Kenichi Funamoto, Toshiyuki Hayase

    Proceedings of the 8th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration 74-75 2008/12

  180. Frictional characteristics of erythrocytes on coated glass plates subject to inclined centrifugal forces Peer-reviewed

    Takashi Kandori, Toshiyuki Hayase, Kousuke Inoue, Kenichi Funamoto, Takanori Takeno, Makoto Ohta, Motohiro Takeda, Atsushi Shirai

    JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME 130 (5) 2008/10

    Publisher: ASME-AMER SOC MECHANICAL ENG

    DOI: 10.1115/1.2948420  

    ISSN:0148-0731

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    In recent years a diamond-like carbon (DLC) film and a 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer have attracted attention as coating materials for implantable artificial organs or devices. When these materials are coated on vascular devices, compatibility to blood is an important problem. The present paper focuses on friction characteristics of erythrocytes to these coating materials in a medium. With an inclined centrifuge microscope developed by the authors, observation was made for erythrocytes moving on flat glass plates with and without coating in a medium of plasma or saline under the effect of inclined centrifugal force. Friction characteristics of erythrocytes with respect to these coating materials were then measured and compared to each other to characterize DLC and MPC as coating materials. The friction characteristics of erythrocytes in plasma using the DLC-coated and noncoated glass plates are similar, changing approximately proportional to the 0.5th power of the cell velocity. The cells stick to these plates in saline as well, implying the influence of plasma protein. The results using the MPC-coated plate in plasma are similar to those of the other plates for large cell velocities, but deviate from the other results with decreased cell velocity. The results change nearly proportional to the 0.75th power of the cell velocity in the range of small velocities. The results for the MPC-coated plate in saline are similar to that in plasma but somewhat smaller, implying that the friction characteristics for the MPC-coated plate are essentially independent of plasma protein.

  181. Numerical experiment for Ultrasonic-Measurement-Integrated simulation of three-dimensional unsteady blood flow Peer-reviewed

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    ANNALS OF BIOMEDICAL ENGINEERING 36 (8) 1383-1397 2008/08

    Publisher: SPRINGER

    DOI: 10.1007/s10439-008-9519-7  

    ISSN:0090-6964

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    Integration of ultrasonic measurement and numerical simulation is a possible way to break through limitations of existing methods for obtaining complete information on hemodynamics. We herein propose Ultrasonic-Measurement-Integrated (UMI) simulation, in which feedback signals based on the optimal estimation of errors in the velocity vector determined by measured and computed Doppler velocities at feedback points are added to the governing equations. With an eye towards practical implementation of UMI simulation with real measurement data, its efficiency for three-dimensional unsteady blood flow analysis and a method for treating low time resolution of ultrasonic measurement were investigated by a numerical experiment dealing with complicated blood flow in an aneurysm. Even when simplified boundary conditions were applied, the UMI simulation reduced the errors of velocity and pressure to 31% and 53% in the feedback domain which covered the aneurysm, respectively. Local maximum wall shear stress was estimated, showing both the proper position and the value with 1% deviance. A properly designed intermittent feedback applied only at the time when measurement data were obtained had the same computational accuracy as feedback applied at every computational time step. Hence, this feedback method is a possible solution to overcome the insufficient time resolution of ultrasonic measurement.

  182. Development of Material Close to Anatomy: Experimental Apparatus to Measure Acoustic Properties

    Osamu Yamashita, Kenichi Funamoto, Toshiyuki Hayase

    Proceedings of Tohoku-NUS Student Joint Symposium 6-7 2008/05

  183. Numerical Evaluation of Ultrasonic-Measurement-Integrated Simulation of Blood Flow

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    Proceedings of 5th International Bio-Fluid Symposium and Workshop 116-117 2008/03

  184. Numerical Experiment for Ultrasonic-Measurement-Integrated Simulation of Developed Laminar Pipe Flow Using Axisymmetric Model Peer-reviewed

    Lei Liu, Kenichi Funamoto, Toshiyuki Hayase

    Journal of Biomechanical Science and Engineering 3 (2) 101-115 2008

    DOI: 10.1299/jbse.3.101  

    ISSN:1880-9863

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    Many studies have been carried out on the relationship between the occurrence and progression of circulatory diseases and hemodynamics. However, it is difficult to obtain accurate and detailed information on blood flow in the living body with existing experimental and numerical methods. The authors have previously proposed the Ultrasonic-Measurement-Integrated (UMI) simulation and shown that the blood flow in an aortic aneurysm can be accurately reproduced when feedback signals derived from the difference between measured and computed Doppler velocities are fed back to the numerical simulation. In the present study, we performed a fundamental numerical experiment in which UMI simulation was applied to a developed laminar pipe flow using an axisymmetric model in order to understand the effect of the feedback law on the accuracy of UMI simulation systematically. The effect of two types of ultrasonic probes, the linear scanning type and the sector scanning type, and the effect of 70 ° and 110 ° irradiating angles of the ultrasonic beam in the linear probe were investigated. It was confirmed that the result of UMI simulation asymptotically approached the standard solution of developed laminar flow downstream of the feedback domain in all cases using the linear probe and the sector probe with axisymmetric feedback. Under the present conditions, a linear probe with a radiation angle of 70 ° was most effective, whereas there was not so much improvement in the accuracy in the case using the sector probe. The effect of the singularity of the axisymmetric coordinate on the pipe axis was observed in the axial velocity profile near the entrance of the feedback domain, but disappeared some distance downstream in that domain. © 2008, The Japan Society of Mechanical Engineers. All rights reserved.

  185. Effect of Time Resolution of Measurement on Ultrasonic-Measurement-Integrated Simulation of Three-Dimensional Unsteady Blood Flow in an Aneurysmal Aorta

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    Proceedings of the 3rd Tohoku-NUS Joint Symposium on Nano-Biomedical Engineering in the East Asian-Pacific Rim Region 13-16 2007/12

  186. Three-Dimensional Flow Simulation around a Deformed Red Blood Cell Moving on a Flat Plate

    Yuki Okuyama, Toshiyuki Hayase, Kenichi Funamoto

    Proceedings of the 7th International Symposium on Advanced Fluid Information and the 4th International Symposium on Transdisciplinary Fluid Integration (AFI/TFI-2007) 60-61 2007/12

  187. MR-Measurement-Integrated Simulation of Blood Flow in a Cerebral Aneurysm

    Yoshitsugu Suzuki, Kenichi Funamoto, Toshiyuki Hayase, Takashi Kosugi, Haruo Isoda

    Proceedings of the 7th International Symposium on Advanced Fluid Information and the 4th International Symposium on Transdisciplinary Fluid Integration (AFI/TFI-2007) 58-59 2007/12

  188. ナノバブルと高周波超音波による腫瘍内血管の三次元画像の構築(Construction of three-dimentional image of intratumoral vessel using high-frequency contrast-enhanced micro-ultrasound)

    冨田 典子, 堀江 佐知子, 渡邊 夕紀子, 船本 健一, 早瀬 敏幸, 大木 宏介, 森川 秀広, 小野 栄夫, 森 士朗, 小玉 哲也

    日本癌学会総会記事 66回 353-353 2007/08

    Publisher: 日本癌学会

    ISSN:0546-0476

  189. Numerical Realization of Blood Flow in Aneurysmal Aorta by Integrating Measurement and Simulation

    Toshiyuki Hayase, Kenichi Funamoto, Takayuki Yamagata, Lei Liu, Atsushi Shirai, Makoto Ohta, Kosuke Inoue, Yoshifumi Saijo, Tomoyuki Yambe

    Future Medical Engineering Based on Bionanotechnology 857-868 2007

  190. Accuracy of Ultrasonic-Measurement-Integrated simulation for three-dimensional blood flow in aneurysmal aorta Peer-reviewed

    K. Funamoto, T. Hayase, Y. Saijo, T. Yambe

    FEDSM 2007: PROCEEDINGS OF THE 5TH JOINT ASME/JSME FLUIDS ENGINEERING SUMMER CONFERENCE, VOL 2, PTS A AND B 733-738 2007

    Publisher: AMER SOC MECHANICAL ENGINEERS

    DOI: 10.1115/FEDSM2007-37497  

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    The UItrasonic-Measurement-Integrated (UMI) simulation, in which feedback signals proportional to the optimal estimation of the difference in velocity vector against real blood flow obtained by Doppler velocities are applied at grid points in the feedback domain, has been developed to reproduce complicated hemodynamics. This paper evaluates UMI simulation for three-dimensional unsteady blood flow in aneurysmal aorta. We performed a numerical experiment of UMI simulation with an unsteady standard solution of the three-dimensional blood flow in an aneurysmal aorta with a realistic boundary condition. The UMI simulation was performed with an inaccurate boundary condition and various feedback arrangements assuming the acquisition of Doppler velocities in the aneurysmal domain or feedback domain. The application of feedback made the computational result approach to the standard Solution, so that UMI simulation estimated the hemodynamic stresses more correctly than the ordinary simulation. Hence, UMI Simulation of real blood flows will bring significant benefits for the clinical diagnosis and treatment of circulatory diseases.

  191. Numerical study on variation of feedback methods in ultrasonic-measurement-integrated simulation of blood flow in the aneurysmal aorta Peer-reviewed

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing 49 (1) 144-155 2006/09/15

    DOI: 10.1299/jsmec.49.144  

    ISSN:1344-7653 1347-538X

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    The complicated relationships between hemodynamics and aneurysms have been investigated intensively. However, existing methodologies have inherent limitations in providing real blood flow fields. The authors have proposed Ultrasonic-Measurement-Integrated (UMI) simulation, in which the feedback signals lead to convergence of the calculated blood flow structure to the real one even with incorrect boundary/initial conditions. In UMI simulation, determination of the feedback law is substantially important, but detailed particulars remain to be accounted for. In this paper, first, the effects of density of feedback points and feedback domains are systematically investigated. Improvement of computational accuracy in the feedback domain is achieved even in low density of feedback points of 25%, and such improvement persists in the downstream region. Secondly, the most effective combination of feedback gains for momentum and pressure equations is investigated, confirming the validity of the simple condition to use the same value for the velocity and pressure gains. Copyright © 2006 by The Japan Society of Mechanical Engineers.

  192. Improvement of Accuracy of Blood Flow Simulation by Integrating Measurement Using Ultrasonic Doppler Method

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    Proceedings of the 5th World Congress of Biomechanics CD-ROM 2006/07

  193. Fundamental Study of Transient Characteristics of Ultrasonic-Measurement-Integrated Simulation toward Reproduction of Unsteady Blood Flows

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    Proceedings of ASME 2006 Summer Bioengineering Conference CD-ROM 2006/06

  194. 637 Blood Flow Simulation Integrated with Ultrasonic Measurement : 6^<th> Report : Three-Dimensional Unsteady Blood Flow Simulation

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    The proceedings of the JSME annual meeting 2006 195-196 2006

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmemecjo.2006.5.0_195  

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    Development and progress of circulatory diseases are closely related to the hemodynamics in the blood vessel. However, detailed and accurate information of blood flow field has yet to be obtained due to the limitations both in measurement and numerical simulation. Hence, we have proposed the Ultrasonic-Measurement-Integrated (UMI) simulation. In this method, feedback signals derived from the difference between measured and computed Doppler velocities are fed back to the numerical simulation. The transient and steady characteristics of the UMI simulation have been revealed. In this paper, we performed the numerical experiment aiming at the reproduction of the three-dimensional unsteady flow field. As the result, the application of the feedback in the UMI simulation made the computational results converge and trace to the model of real blood flow.

  195. S-14 超音波による心臓・血管の中の流れの医工学的解析(特別企画II(要望演題5) 逆流性疾患・閉塞性疾患・拡張性疾患に対する治療戦略-管と流れと-,第43回 日本小児外科学会総会)

    西條 芳文, 白石 泰之, 関根 一光, 山家 智之, 田中 元直, 田林 晄一, 早瀬 敏幸, 船本 健一, 大槻 茂雄, 菅原 重生

    日本小児外科学会雑誌 42 (3) 355-355 2006

    Publisher: 特定非営利活動法人 日本小児外科学会

    DOI: 10.11164/jjsps.42.3_355_2  

  196. Blood Flow Simulation Coupled with Ultrasonic Measurement

    Toshiyuki Hayase, Kenichi Funamoto

    Proceedings of the 1st Japan Korea Student Workshop 2 2006

  197. Effect of Feedback Domain in Ultrasonic-Measurement-Integrated Simulation on Reproduction of Blood Flow Field in an Aneurysmal Aorta

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    Proceedings of the 6th International Symposium on Future Medical Engineering based on Bio-nanotechnology (21st Century COE Program) 62-63 2005/11

  198. Ultrasonic-Measurement-Integrated Simulation for Reproduction of Three-Dimensional Blood Flow Field in the Aorta with Aneurysm

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    Proceedings of ASME 2005 Summer Bioengineering Conference CD-ROM 2005/06

  199. Fundamental study of ultrasonic-measurement-integrated simulation of real blood flow in the aorta Peer-reviewed

    K Funamoto, T Hayase, A Shirai, Y Saijo, T Yambe

    ANNALS OF BIOMEDICAL ENGINEERING 33 (4) 415-428 2005/04

    Publisher: SPRINGER

    DOI: 10.1007/s10439-005-2495-2  

    ISSN:0090-6964

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    Acquisition of detailed information on the velocity and pressure fields of the blood flow is essential to achieve accurate diagnosis or treatment for serious circulatory diseases such as aortic aneurysms. A possible way to obtain such information is integration of numerical simulation and color Doppler ultrasonography in the framework of a flow observer. This methodology, namely, Ultrasonic-Measurement-Integrated (UMI) Simulation, consists of the following processes. At each time step of numerical simulation, the difference between the measurable output signal and the signal indicated by numerical simulation is evaluated. Feedback signals are generated from the difference, and numerical simulation is updated applying the feedback signal to compensate for the difference. This paper deals with a numerical study on the fundamental characteristics of UMI simulation using a simple two-dimensional model problem for the blood flow in an aorta with an aneurysm. The effect of the number of feedback points and the feedback formula are investigated systematically. It is revealed that the result of UMI simulation in the feedback domain rapidly converges to the standard solution, even with usually inevitable incorrect upstream boundary conditions. Finally, an example of UMI simulation with feedback from real color Doppler measurement also shows a good agreement with measurement.

  200. Fundamental study of ultrasonic-measurement-integrated simulation of real blood flow in the aorta Peer-reviewed

    K Funamoto, T Hayase, A Shirai, Y Saijo, T Yambe

    ANNALS OF BIOMEDICAL ENGINEERING 33 (4) 415-428 2005/04

    Publisher: SPRINGER

    DOI: 10.1007/s10439-005-2495-2  

    ISSN:0090-6964

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    Acquisition of detailed information on the velocity and pressure fields of the blood flow is essential to achieve accurate diagnosis or treatment for serious circulatory diseases such as aortic aneurysms. A possible way to obtain such information is integration of numerical simulation and color Doppler ultrasonography in the framework of a flow observer. This methodology, namely, Ultrasonic-Measurement-Integrated (UMI) Simulation, consists of the following processes. At each time step of numerical simulation, the difference between the measurable output signal and the signal indicated by numerical simulation is evaluated. Feedback signals are generated from the difference, and numerical simulation is updated applying the feedback signal to compensate for the difference. This paper deals with a numerical study on the fundamental characteristics of UMI simulation using a simple two-dimensional model problem for the blood flow in an aorta with an aneurysm. The effect of the number of feedback points and the feedback formula are investigated systematically. It is revealed that the result of UMI simulation in the feedback domain rapidly converges to the standard solution, even with usually inevitable incorrect upstream boundary conditions. Finally, an example of UMI simulation with feedback from real color Doppler measurement also shows a good agreement with measurement.

  201. Effect of Density of Monitoring Points for Feedback in Ultrasonic-Measurement-Integrated Simulation of Blood Flow in the Aorta with Aneurysm Peer-reviewed

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    Proceedings of the 3rd IASTED International Conference on Biomedical Engineering (BioMED2005) 118-123 2005/02

  202. 1108 Blood Flow Simulation Integrated with Ultrasonic Measurement : 4^<th> Report: Improvement of Accuracy of Computational Hemodynamics

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    The proceedings of the JSME annual meeting 2005 89-90 2005

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmemecjo.2005.5.0_89  

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    Hemodynamics plays an important role in the development, progress and rupture of aneurysms, and therefore, is essential for advanced diagnosis. However, existing methodologies have yet to provide detailed and accurate information of blood flow field due to their individual limitations. As a solution to the problem, we have proposed Ultrasonic-Measurement-Integrated (UMI) simulation. In this method, feedback signals derived from the difference between measured Doppler velocity and the calculated one by numerical simulation are fed back to the numerical simulation. In this paper, we validate the efficacy of three-dimensional UMI simulation by the numerical analysis with various irradiation methods of ultrasonic beam. As the result, UMI simulation improves computational accuracy and enables us to obtain more accurate information of blood flow field including wall shear stress.

  203. 410 Study of Three-dimensional Feedback Law for Ultrasonic-Measurement-Integrated Simulation of Blood Flow in the Aorta

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    Proceedings of the JSME Bioengineering Conference and Seminar 2004 151-152 2005

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmebs.2004.17.0_151  

  204. Detection and correction of aliasing in ultrasonic measurement of blood flows with Ultrasonic-Measurement-Integrated simulation Peer-reviewed

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    Technology and Health Care 13 (4) 331-344 2005

    ISSN:0928-7329

    More details Close

    Detailed information of real blood flows is essential to develop an accurate diagnosis or treatment for serious circulatory diseases such as aortic aneurysms. Ultrasonic-Measurement-Integrated (UMI) simulation, in which feedback signals from the ultrasonic measurement make the simulation converge to the real blood flow, is a key to solving this problem. However, aliasing in the ultrasonic blood velocity measurement causes UMI simulation to converge to an erroneous result. In this paper, we have investigated the detection and the correction of aliasing in UMI simulation. The artificial force in the feedback of UMI simulation can be used as an index to detect the aliasing. We have proposed two ways for the correction of the aliasing. Correction A, in which measurement velocity is replaced with the computational one at the monitoring point where the aliasing is detected, substantially improves the accuracy of UMI simulation. Correction B, in which measurement velocity is replaced with an estimated Doppler velocity, can provide exactly the same result as that of UMI simulation using the nonaliased standard solution. Although correction B gives the most accurate result, correction A seems more robust and, therefore, a beneficial choice considering the other artifacts in the measurement. © 2005 - IOS Press and the authors. All rights reserved.

  205. Measurement-Integrated Simulation for Numerical Realization of Complex Bio-Fluid Systems

    Toshiyuki Hayase, Kenichi Funamoto, Atsushi Shirai

    Proceedings of the 2nd International Symposium on Intelligent Artifacts and Bio-Systems, 2nd INABIO 65 2005

  206. Transdisciplinary Methodology Integrating Measurement and Simulation in Application of Complex Biomedical Flows

    Toshiyuki Hayase, Kenichi Funamoto, Takayuki Yamagata, Lei Liu, Atsushi Shirai, Makoto Ohta, Kosuke Inoue, Yoshifumi Saijo, Tomoyuki Yambe

    Proceedings of the 5th International Symposium on Advanced Fluid information (AFI-2005) 36-39 2005

  207. Ultrasonic-Measurement-Integrated Simulation of Blood Flow in the Aorta with an Aneurysm

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    Proceedings of the 4th International Symposium on Advanced Fluid Information and the 1st International Symposium on Transdisciplinary Fluid Integration (AFI/TFI-2004) 16-21 2004/11

  208. Application of Ultrasonic-Measurement-Integrated Simulation to Blood Flow

    Kenichi Funamoto, Toshiyuki Hayase, Atsushi Shirai, Yoshifumi Saijo, Tomoyuki Yambe

    Proceedings of the 4th International Symposium on Future Medical Engineering based on Bio-nanotechnology 78-79 2004/06

  209. Analysis of Blood Flow in Aorta with Ultrasonic-Measurement-Integrated Simulation

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    The proceedings of the JSME annual meeting 2004 39-40 2004

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmemecjo.2004.7.0_39  

    More details Close

    In order to reproduce the blood velocity and pressure fields of the blood flow for an accurate diagnosis or treatment for serious circulatory diseases such as aortic aneurysms, we are developing a new analysis methodology, namely Ultrasonic-Measurement-Integrated (UMI) simulation, by integrating color Doppler ultrasonography and numerical simulation. In the UMI simulation, feedback signals are added to the governing equations to compensate for the difference between computation and measurement. In this paper, we perform UMI simulation for the blood flow in descending aorta with an aneurysm. It is revealed that the UMI simulation reproduces the complicated blood flow structure and detects the incorrect measurement data.

  210. Basic Study of Ultrasonic-Measurement-Integrated Simulation System

    YAMAGATA Takayuki, Funamoto Kenichi, HAYASE Toshiyuki

    The proceedings of the JSME annual meeting 2004 57-58 2004

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmemecjo.2004.5.0_57  

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    Detailed information of the velocity and pressure field of the blood flow is essential to establish an advanced diagnosis and treatment of serious circulatory diseases. Commonly used medical measurement equipments cannot measure such information, while even state-of-the-art numerical simulation cannot reproduce real blood flow accurately. In order to overcome this problem, the authors have proposed a new technique, Ultrasonic-Measurement-Integrated (UMI) simulation, that integrates ultrasonic measurement equipment and numerical, simulation, and revealed its usefulness by numerical simulation. The present report describes the development of fundamental procedure of the interface between ultrasonic equipment and simulation, and acceleration of computational speed for real time operation. By considering the tolerance of the solution, computational speed has been accelerated by a factor of 90 achieving only ten times longer computational time than real phenomena.

  211. Reproduction of Real Blood Flow in Aorta with Ultrasonic-Measurement-Integrated Simulation

    Kenichi Funamoto, Toshiyuki Hayase, Atsushi Shirai, Tomoyuki Yambe, Yoshifumi Saijo

    Proceedings of the 3rd International Symposium on Advanced Fluid Information (AFI-2003) 39-40 2003/11

  212. Evidence-based Simulation of Blood Flow with Feedback of Ultrasonic Measurement

    Toshiyuki Hayase, Kenichi Funamoto, Atsushi Shirai, Tomoyuki Yambe, Yoshifumi Saijo

    The 1st International Symposium on Future Medical Engineering based on Bio-nanotechnology 40-42 2003/01

  213. Two-dimensional computational flow analysis and frictional characteristics model for red blood cell under inclined centrifuge microscopy Peer-reviewed

    Kenichi Funamoto, Toshiyuki Hayase, Atsushi Shirai

    JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing 46 (4) 1304-1311 2003

    Publisher: Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmec.46.1304  

    ISSN:1344-7653

    More details Close

    Simplified two-dimensional flow analysis is performed in order to simulate frictional characteristics measurement of red blood cells moving on a glass plate in a medium with an inclined centrifuge microscope. Computation under various conditions reveals the influences of parameters on lift, drag, and moment acting on a red blood cell. Among these forces, lift appears only when the cell is longitudinally asymmetric. By considering the balance of forces, the frictional characteristics of the red blood cell are modeled as the sum of Coulomb friction and viscous drag. The model describes the possibility that the red blood cell deforms to expand in the front side in response to the inclined centrifugal force. When velocity exceeds some critical value, the lift overcomes the normal centrifugal force component, and the thickness of the plasma layer between the cell and the glass plate increases from the initial value of the plasma protein thickness.

  214. Numerical Realization of Flow by Integrating Computation and Measurement in Medical Applications Invited

    Toshiyuki Hayase, Kenichi Funamoto, Keisuke Nisugi, Atsushi Shirai, Tomoyuki Yambe, Yoshifumi Saijo

    Proceedings of Mini-Symposium on Advanced Fluid Information: Fusion of EFD and CFD 77-85 2002/12

  215. Numerical Simulation of Red Blood Cell under Inclined Centrifugal Force

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SHIRAI Atsushi

    The proceedings of the JSME annual meeting 2002 97-98 2002

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmemecjo.2002.6.0_97  

    More details Close

    Two dimensional flow analysis was performed to simulate the former experiment using the inclined centrifuge microscope for the frictional characteristics of red blood cells moving on a glass plate in a medium. Lift, drag and momentum acting on a red blood cell were obtained in various conditions. Considering a balance among various forces acting on a red blood cell, the frictional characteristics of a red blood cell were modeled as the sum of Coulomb friction and drag. The result indicates the possibility that a red blood cell deforms to expand on the front side, and the plasma layer of the thickness of 0.16 μm exists on a glass plate.

Show all ︎Show first 5

Misc. 75

  1. マイクロ流体デバイスにより解明される細胞性粘菌の酸素に対する走性 Invited

    廣瀬 理美, 船本 健一

    アグリバイオ 6 (7) 90-93 2022/07/20

  2. マイクロ流体デバイスを用いた真核細胞モデルの走気性の観察 Invited

    廣瀬 理美, 船本 健一

    細胞 54 (7) 38-41 2022/06/20

  3. 酸素濃度制御マイクロ流体デバイスによる細胞動態の観察 Invited

    荒武 聖, 船本 健一

    細胞 54 (3) 47-50 2022/02/22

  4. Preface

    Takashi Tokumasu, Hitomi Anzai, Koji Fujita, Kenichi Funamoto, Makoto Hirota, Hisashi Nakamura, Koji Shimoyama, Hidemasa Takana

    JOURNAL OF FLUID SCIENCE AND TECHNOLOGY 16 (1) 2021

    Publisher: JAPAN SOC MECHANICAL ENGINEERS

    DOI: 10.1299/jfst.2021jfst0001  

    ISSN:1880-5558

  5. Microfluidic device to reproduce in vivo hypoxic microenvironments Invited

    Kenichi Funamoto

    The Cell 52 (4) 50-53 2020/03/20

  6. 二次元超音波計測融合血流解析における超音波計測のスペックルノイズの影響

    門脇 弘子, 早瀬 敏幸, 船本 健一, 宮内 優, 井上 浩介, 島崎 正, 地挽 隆夫, 見山 広二

    超音波テクノ 28 (3) 15-20 2016/06/01

    Publisher: 日本工業出版株式会社

  7. 2D34 Investigation of Permeability of Endothelial Cell Monolayer by Using Microfluidic Device with Controllability of Oxygen Tension

    MATSUBARA Kento, FUNAMOTO Kenichi, ZERVANTONAKIS Ioannis K., FUNAMOTO Kiyoe, ITO Takuya, MASAMUNE Jun, KIMURA Yoshitaka, HAYASE Toshiyuki, KAMM Roger D.

    2016 (28) "2D34-1"-"2D34-5" 2016/01/09

    Publisher: The Japan Society of Mechanical Engineers

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    Permeability changes of an endothelial cell (EC) monolayer are related to many diseases. This study evaluated permeability of the EC monolayer under normoxia and hypoxia by using fluorescent dextrans of two different molecular weights in a microfluidic device with controllability of oxygen tension. As the results, permeability of the EC monolayer increased by hypoxic exposure, and the increase of the permeability measured by fluorescent dextran of 10 kDa was smaller than that measured by fluorescent dextran of 70 kDa. The results indicated that the size-selectivity of EC monolayer became weaken under hypoxia.

  8. B106 Measurement of permeability of endothelial monolayer under hypoxia by using microfluidic device

    MATSUBARA Kento, FUNAMOTO Kenichi, ZERVANTONAKIS Ioannis K., FUNAMOTO Kiyoe, ITO Takuya, MASAMUNE Jun, KIMURA Yoshitaka, HAYASE Toshiyuki, KAMM Roger D.

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2015 (26) 35-36 2015/10/01

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  9. 2A24 Investigation of setting of upstream boundary in computational hemodynamic analysis in a cerebral aneurysm

    SUZUKI Daichi, FUNAMOTO Kenichi, SUGIYAMA Shin-ichiro, HAYASE Toshiyuki, TOMINAGA Teiji

    2015 (27) 317-318 2015/01/08

    Publisher: The Japan Society of Mechanical Engineers

  10. 1D42 Measurement of frictional characteristics of erythrocytes on endothelial cells with an inclined centrifuge microscope : influence of normal component of inclined centrifugal force

    INOUE Kosuke, HAYASE Toshiyuki, FUNAMOTO Kenichi, SHIRAI Atsushi

    2015 (27) 169-170 2015/01/08

    Publisher: The Japan Society of Mechanical Engineers

  11. 1B11 Investigation on reproduction of pressure field by three-dimensional unsteady ultrasonic-measurement-integrated blood flow simulation

    FUNAMOTO Kenichi, HAYASE Toshiyuki

    2015 (27) 49-50 2015/01/08

    Publisher: The Japan Society of Mechanical Engineers

  12. 1B13 Experimental validation of analysis accuracy in two-dimensional ultrasonic-measurement-integrated simulation system

    KADOWAKI Hiroko, HAYASE Toshiyuki, FUNAMOTO Kenichi, INOUE Kosuke, SHIMAZAKI Tadashi, JIBIKI Takao, MIYAMA Koji

    2015 (27) 53-54 2015/01/08

    Publisher: The Japan Society of Mechanical Engineers

  13. 1C34 Evaluation of permeability of endothelial monolayer by using hypoxia microfluidic device

    MATSUBARA Kento, FUNAMOTO Kenichi, Zervantonakis Ioannis, FUNAMOTO Kiyoe, ITO Takuya, MASAMUNE Jun, KIMURA Yoshitaka, HAYASE Toshiyuki, KAMM Roger

    2015 (27) 121-122 2015/01/08

    Publisher: The Japan Society of Mechanical Engineers

  14. 超音波計測と計測融合シミュレーションによる血圧場と血流場の同時解析システム

    曾根周作, 早瀬敏幸, 船本健一, 白井敦

    バイオエンジニアリング講演会講演論文集 27th 2015

    ISSN:1348-2920

  15. Twinkling Sign機序解明のための超音波照射による微粒子挙動の光学観察

    LIU LEI, FUNAMOTO KEN'ICHI, TANABE MASAYUKI, HAYASE TOSHIYUKI

    超音波医学 41 (Suppl.) S449-S449 2014/04/15

    Publisher: (一社)日本超音波医学会

    ISSN:1346-1176

  16. ソノサイトメトリーの基盤技術の検討

    小松 洋介, 長岡 亮, 船本 健一, 早瀬 敏幸, 政氏 伸夫, 金井 浩, 西條 芳文

    超音波医学 41 (Suppl.) S481-S481 2014/04

    Publisher: (公社)日本超音波医学会

    ISSN:1346-1176

  17. 1C22 Angiogenic remodeling of extracellular matrix in microfluidic device

    NAGASUGA Daisuke, FUKUSHIMA Shuichiro, FUNAMOTO Kenichi, ARAKI Tsutomu

    2014 (26) 79-80 2014/01/10

    Publisher: The Japan Society of Mechanical Engineers

  18. 1G11 Comparison of hemodynamic parameters and wall characteristics of cerebral aneurysms

    SUZUKI Daichi, FUNAMOTO Kenichi, SUGIYAMA Shin-ichiro, NAKAYAMA Toshio, HAYASE Toshiyuki, TOMINAGA Teiji

    2014 (26) 207-208 2014/01/10

    Publisher: The Japan Society of Mechanical Engineers

  19. 2B25 Validations of 3D blood vessel reconstruction and blood flow analysis by ultrasonic-measurement-integrated flow-structure interaction simulation system for small animals

    SAKANISHI Sanga, HAYASE Toshiyuki, FUNAMOTO Kenichi, SONE Shusaku

    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2014 (26) 309-310 2014/01/10

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmebio.2014.26.309  

  20. 2B46 The influence of axial variation of the plane flow rate on two-dimensional ultrasonic-measurement-integrated simulation of blood flow in a common carotid artery

    MATSUMOTO Takuya, FUNAMOTO Kenichi, HAYASE Toshiyuki

    2014 (26) 335-336 2014/01/10

    Publisher: The Japan Society of Mechanical Engineers

  21. 168 Fundamental Study of Interaction between Red Blood Cell and Endothelial Cell under Inclined Centrifugal Force : Reproduction of Friction Characteristics by Numerical Analysis Using Interaction Model

    YATSUYANAGI Akira, HAYASE Toshiyuki, INOUE Kosuke, FUNAMOTO Kenichi, SHIRAI Atsushi

    2014 (49) 133-134 2014

    Publisher: The Japan Society of Mechanical Engineers

  22. MR計測融合シミュレーションによる脳動脈瘤破裂予測

    杉山慎一郎, 船本健一, 早瀬敏幸, 清水宏明, 冨永悌二

    東北大学流体科学研究所共同利用・共同研究拠点流体科学研究拠点活動報告書 2013 2014

  23. 軟組織内微細石灰化Twinkling Signに関する実験的研究:粒子径,粒子種類が与える影響

    LIU LEI, FUNAMOTO KEN'ICHI, TANABE MASAYUKI, HAYASE TOSHIYUKI

    超音波医学 40 (Suppl.) S408-S408 2013/04/15

    Publisher: (一社)日本超音波医学会

    ISSN:1346-1176

  24. 3D06 Observation of behavior of cancer cells under hypoxia using microfluidic device

    FUNAMOTO Kenichi, ZERVANTONAKIS Ioannis K.

    2013 (25) 563-564 2013/01/08

    Publisher: The Japan Society of Mechanical Engineers

  25. 3C14 Comparison of three-dimensional blood flow analysis in cerebral aneurysm with MRI measurement

    SUZUKI Daichi, FUNAMOTO Kenichi, HAYASE Toshiyuki, SUGIYAMA Shin-ichiro

    2013 (25) 547-548 2013/01/08

    Publisher: The Japan Society of Mechanical Engineers

  26. 2A08 Validation of two-dimensional ultrasonic-measurement-integrated simulation for three-dimensional blood flow in common carotid artery

    MATSUMOTO Takuya, FUNAMOTO Kenichi, HAYASE Toshiyuki

    2013 (25) 247-248 2013/01/08

    Publisher: The Japan Society of Mechanical Engineers

  27. 2A10 Analysis of blood flow in mouse carotid artery by ultrasonic-measurement-integrated flow-structure interaction simulation system for small animal

    SAKANISHI Sanga, HAYASE Toshiyuki, FUNAMOTO Kenichi, SONE Shusaku

    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2013 (25) 251-252 2013/01/08

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmebio.2013.25.251  

  28. 2A09 Estimation of Blood Viscosity Based on Ultrasonic-Measurement-Integrated Simulation

    KADOWAKI Hiroko, FUNAMOTO Kenichi, HAYASE Toshiyuki, SONE Shusaku, OGASAWARA Masafumi, JIBIKI Takao, HASHIMOTO Hiroshi, MIYAMA Koji, LIU Lei

    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2013 (25) 249-250 2013/01/08

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmebio.2013.25.249  

  29. 超音波Mモードイメージングと光電容積脈波センシングによる脈波同定の比較

    曾根周作, 早瀬敏幸, 船本健一

    超音波医学 40 2013

    ISSN:1346-1176

  30. 超音波計測融合シミュレーションによる生体内の血液粘度の推定

    門脇弘子, 船本健一, 早瀬敏幸, 曾根周作, 地挽隆夫, 橋本浩, 見山広二, LIU Lei

    超音波医学 40 2013

    ISSN:1346-1176

  31. MR計測融合シミュレーションによる脳動脈瘤破裂予測

    杉山慎一郎, 船本健一, 早瀬敏幸, 清水宏明, 冨永悌二

    東北大学流体科学研究所共同利用・共同研究拠点流体科学研究拠点活動報告書 2012 2013

  32. OS3-2-2 Microfluidic Device to Control Oxygen Tension in Cellular Experiment

    Funamoto Kenichi, Zervantonakis Ioannis K., Liu Yuchun, Kamm Roger D.

    2012 (4) 77-78 2012/10/21

    Publisher: The Japan Society of Mechanical Engineers

    More details Close

    Hypoxic microenvironment affects cell fate, and regulates various physiological and pathological phenomena. We designed a microfluidic device to control oxygen tension for three-dimensional cell culture. A polycarbonate (PC) film was embedded in the device to overcome oxygen diffusion from the top surface. Numerical simulations were performed to investigate changes of oxygen tension upon varying the media and gas flow rates. In addition, oxygen tension created based on the computational optimization was validated by using a ruthenium-coated oxygen-sensing glass cover slip. The computational results revealed that there was the optimum combination of the flow rates of media and gas to control oxygen tension, showing establishments of a low uniform oxygen tension (<3%) or an oxygen gradient. The experimental results agreed well with the corresponding computational results.

  33. C207 Improvement of extraction of vessel shape in ultrasonic-measurement-integrated flow-structure interaction simulation system for small animal experiment

    Sakanishi Sanga, Hayase Toshiyuki, Funamoto Kenichi, Sone Shusaku

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2012 (23) 181-182 2012/10/04

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmebiofro.2012.23.181  

    ISSN:1348-2939

  34. A103 Blood Flow Analysis in Carotid Bifurcation with Two-Dimensional Ultrasonic-Measurement-Integrated Simulation

    Kadowaki Hiroko, Funamoto Kenichi, Hayase Toshiyuki, Sone Shusaku, Ogasawara Masafumi, Jibiki Takao, Hashimoto Hiroshi, MIYAMA Koji

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2012 (23) 5-6 2012/10/04

    Publisher: The Japan Society of Mechanical Engineers

    DOI: 10.1299/jsmebiofro.2012.23.5  

    ISSN:1348-2939

  35. Visual Synergetics(9)Realization of Blood Flow Dynamics based on Visual Synergetics

    17 4p 2012/05

    Publisher: 日本計算工学会

  36. 8F24 Numerical Analysis of Levitation Mechanism of Red Blood Cell in Inclined Centrifuge Microscope : 2nd Report: Validity of Rigid Red Blood Cell Model for Reproduction of Friction Characteristics

    OSHIBE Takashi, HAYASE Toshiyuki, FUNAMOTO Kenichi, SHIRAI Atsushi

    2012 (24) "8F24-1"-"8F24-2" 2012/01/06

    Publisher: The Japan Society of Mechanical Engineers

  37. 頸動脈分岐部内血流の2次元超音波計測融合シミュレーョン

    門脇弘子, 船本健一, 早瀬敏幸, 曾根周作, 小笠原正文, 地挽隆夫, 橋本浩, 見山広二

    バイオエンジニアリング講演会講演論文集 24th (CD-ROM) 2012

    ISSN:1348-2920

  38. 2次元超音波計測融合シミュレーションによる頸動脈分岐部の非定常血流量推定

    門脇弘子, 船本健一, 早瀬敏幸, 曾根周作, 小笠原正文, 地挽隆夫, 橋本浩, 見山広二

    超音波医学 39 2012

    ISSN:1346-1176

  39. MR計測融合シミュレーションによる脳動脈瘤破裂予測

    杉山慎一郎, 船本健一, 清水宏明, 冨永悌二, 早瀬敏幸

    東北大学流体科学研究所共同利用・共同研究拠点流体科学研究拠点活動報告書 2011 2012

  40. B120 Numerical Analysis of Levitation Mechanism of Red Blood Cell in Inclined Centrifuge Microscope

    OSHIBE Takashi, HAYASE Toshiyuki, FUNAMOTO Kenichi, SHIRAI Atsushi

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2011 (22) 77-78 2011/10/06

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  41. 超音波計測融合シミュレーションと計測による上流速度を与えたシミュレーションの比較

    曾根周作, 加藤宇海, 船本健一, 早瀬敏幸, 小笠原正文, 地挽隆夫, 橋本浩, 見山広二

    超音波医学 38 2011

    ISSN:1346-1176

  42. 脳動脈瘤破裂点の流体力学的特徴:コンピュータ流体解析による検討

    面高俊介, 井上敬, 船本健一, 杉山慎一郎, 藤村幹, 清水宏明, 早瀬敏幸, 高橋明, 冨永悌二

    脳循環代謝 23 (1) 2011

    ISSN:0915-9401

  43. B215 Evaluation of Wall Shear Stress in Carotid Artery with Ultrasonic-Measurement-Integrated Simulation

    KATO Takaumi, FUNAMOTO Kenichi, HAYASE Toshiyuki, OGASAWARA Masafumi, JIBIKI Takao, HASHIMOTO Hiroshi, MIYAMA Koji

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2010 (21) 113-114 2010/11/11

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  44. B214 Development of Ultrasonic-Measurement-Integrated Simulation System for Small Animal Experiment : Confirmation Experiment

    TANAKA Yuji, FUNAMOTO Kenichi, HAYASE Toshiyuki, INOUE Kosuke

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2010 (21) 111-112 2010/11/11

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  45. A104 Blood Flow Analysis in the Left Atrium with/without Atrial Fibrillation

    KOIZUMI Ryo, FUNAMOTO Kenichi, HAYASE Toshiyuki, SHIBATA Muneichi

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2010 (21) 7-8 2010/11/11

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  46. W205 Blood Flow Simulation Integrated with Medical Measurement

    Funamoto Kenichi, Hayase Toshiyuki

    Fluids engineering conference ... 2010 619-622 2010/10/29

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2882

    More details Close

    Measurement-integrated (MI) simulation, in which feedback signals are applied to the governing equations based on errors between measurement and numerical simulation, has been investigated to solve various problems related to fluid flows. As medical applications of MI simulation, the authors have proposed integration of medical measurement, such as ultrasound Doppler measurement or phase-contrast MRI (PC MRI), of blood flow velocity and a numerical simulation. Effects of feedback of errors in one- or three-directional velocity information between ultrasound Doppler measurement or PC MRI and the numerical simulation were examined by numerical experiments, respectively. Each MI simulation converged to the standard solution and yielded the hemodynamic stresses accurately and in detail.

  47. Fluid-Structure Interaction Simulation of a Red Blood Cell under Inclined Centrifugal Force

    15 (2) 561-564 2010/05

    Publisher: 日本計算工学会

    ISSN:1342-145X

  48. 214 Eigenvalue Analysis of Measurement-Integrated Simulation of Two-Dimensional Blood Flow in the Vinicity of Aneurysm in the Descending Aorta

    Funamoto Takakazu, Hayase Toshiyuki, Imagawa Kentaro, Funamoto Kenichi

    2010 (45) 228-229 2010/03/12

    Publisher: The Japan Society of Mechanical Engineers

  49. 0624 Dynamic Characteristics Analysis of Diseased Circulatory System with Lumped Parameter Model : 2^<nd> Report:Ventricular Septal Defect

    KOIZUMI Ryo, HAYASE Toshiyuki, FUNAMOTO Kenichi

    2009 (22) 279-279 2010/01/08

    Publisher: The Japan Society of Mechanical Engineers

  50. Effect of Feedback of Zeroed Doppler Velocity on Ultrasonic-Measurement-Integrated Simulation of Blood Flow

    Funamoto Kenichi, Hayase Toshiyuki, Saijo Yoshifumi, Yambe Tomoyuki

    2010 216-216 2010

    Publisher: 日本流体力学会

    More details Close

    Ultrasonic-measurement-integrated (UMI) simulation, in which feedback signals are applied to the governing equations based on errors between ultrasonic measurement and numerical simulation, has been investigated for reproduction of the blood flow field. In this study, the effects of wall filter and lack of data, which provide zero Doppler velocity, on UMI simulation were examined by a numerical experiment dealing with the blood flow field in the descending aorta with an aneurysm. Effects of wall filter and lack of data appeared in diastole and in the whole period, respectively. However, by not adding feedback signals where measured Doppler velocities were zero, the computational accuracy of the UMI simulation substantially improved for the sake of the feedback.

  51. B212 Dynamic Characteristics Analysis of Diseased Circulatory System with Lumped Parameter Model 1^<st> Report : Heart Valve Disease

    KOIZUMI Ryo, HAYASE Toshiyuki, FUNAMOTO Kenichi

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2009 (20) 115-116 2009/11/06

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  52. B108 Analysis of Clinical Data with Ultrasonic-Measurement-Integrated Simulation

    KATTO Takaumi, FUNAMOTO Kenichi, HAYASE Toshiyuki, OGASAWARA Masafumi, JIBIKI Takao, HASHIMOTO Hiroshi, MIYAMA Koji

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2009 (20) 43-44 2009/11/06

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  53. B213 Blood Flow Simulation Integrated with Ultrasonic Measurement 9^<th> Report : Effect of Noise in Ultrasonic Measurement

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2009 (20) 117-118 2009/11/06

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  54. 医療計測と数値シミュレーションを融合した血管内血流の解析

    船本 健一, 早瀬 敏幸

    日本可視化情報学会誌 29 (114) 20-26 2009/07

    Publisher: 社団法人可視化情報学会

  55. 計測融合シミュレーションによる血流解析

    船本 健一, 早瀬 敏幸

    日本機械学会流体工学部門ニューズレター 流れ 2009/04

  56. 539 Development of Poly (Vinyl Alcohol) Gel with in vivo Acoustic Properties

    YAMASHITA Osamu, FUNAMOTO Kenichi, HAYASE Toshiyuki

    2008 (21) 451-452 2009/01/22

    Publisher: The Japan Society of Mechanical Engineers

  57. 326 Blood Flow Simulation Integrated with Ultrasonic Measurement 8th Report : Effect of Error in Ultrasonic Measurement

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    2008 (21) 353-354 2009/01/22

    Publisher: The Japan Society of Mechanical Engineers

  58. B406 Development of Material with Acoustic Property Close to Anatomy : Measurement System

    YAMASHITA Osamu, FUNAMOTO Kenichi, HAYASE Toshiyuki

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2008 (19) 141-142 2008/09/22

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  59. B206 Numerical Experiment of MR-Measurement-Integrated Simulation of Unsteady Blood Flow in a Cerebral Aneurysm

    FUNAMOTO Kenichi, SUZUKI Yoshitsugu, HAYASE Toshiyuki, KOSUGI Takashi, ISODA Haruo

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2008 (19) 52-53 2008/09/22

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  60. Visualization of blood flow field by ultrasonic-measurement-integrated simulation

    FUNAMOTO Kenichi, HAYASE Toshiyuki, LIU Lei, OGASAWARA Masafumi, JIBIKI Takao, HASHIMOTO Hiroshi, MIYAMA Koji

    35 S254 2008/04/15

    ISSN:1346-1176

  61. Carotid artery phantom using PVA-H (Poly vinyl alcohol hydro gel) for ultrasonic measurement and visualization

    LIU Lei, HAYASE Toshiyuki, FUNAMOTO Kenichi, OGASAWARA Masafumi, JIBIKI Takao, HASHIMOTO Hiroshi, MIYAMA Kouji

    35 S311 2008/04/15

    ISSN:1346-1176

  62. 132 Ultrasonic-Measurement-Integrated Simulation on Laminar Flow in a Straight Tube : Three-Dimensional Analysis Using Non-Axisymmetric Feedback Law

    FUNAMOTO Kenichi, LIU Lei, HAYASE Toshiyuki

    2007 (20) 271-272 2008/01/24

    Publisher: The Japan Society of Mechanical Engineers

  63. 131 Ultrasonic-Measurement-Integrated Simulation on Laminar Flow in a Straight Tube : Axisymmetric Analysis Using Axisymmetric Feedback Law

    LIU Lei, FUNAMOTO Kenichi, HAYASE Toshiyuki

    2007 (20) 269-270 2008/01/24

    Publisher: The Japan Society of Mechanical Engineers

  64. 309 Blood Flow Simulation Integrated with Ultrasonic Measurement : 7^<th> Report: Effect of Time Resolution of Measurement

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    2006 (19) 68-69 2007/01/06

    Publisher: The Japan Society of Mechanical Engineers

  65. 1306 Numerical Experiment of Ultrasonic-Measurement-Integrated Simulation for Three-Dimensional Unsteady Blood Flow(1)

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    Fluids engineering conference ... 2006 "1306-a" 2006/10/28

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2882

  66. 223 Effect of Anisotropic Feedback Points Arrangement in Three-dimensional Ultrasonic-Measurement-Integrated Simulation

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    2005 (18) 105-106 2006/01/12

    Publisher: The Japan Society of Mechanical Engineers

  67. B205 Blood Flow Simulation Integrated with Ultrasonic Measurement : 5^<th> Report: Reproduction of Three-dimensional Unsteady Blood Flow Field

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2005 (16) 137-138 2005/11/08

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  68. 907 Evaluation of Effectiveness of Feedback Formulae in Three-dimensional Ultrasonic-Measurement-Integrated Simulation of Blood Flow

    Funamoto Kenichi, Hayase Toshiyuki, Saijo Yoshifumi, Yambe Tomoyuki

    Fluids engineering conference ... 2005 139-139 2005/10/28

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2882

  69. AM05-17-010 Effect of Feedback Points Arrangement on Accuracy of Three-dimensional Ultrasonic-Measurement-Integrated Simulation of Blood Flow

    Funamoto Kenichi, Hayase Toshiyuki, Saijo Yoshifumi, Yambe Tomoyuki

    2005 226-226 2005

    Publisher: 日本流体力学会

    More details Close

    We have developed Ultrasonic-Measurement-Integrated (UMI) simulation to obtain detailed and accurate information of blood flow field numerically for the diagnosis or treatment of aneurysms. In this methodology, feedback signals derived from the difference between Doppler velocities by measurement and computation are fed back to the numerical simulation. In this study, we investigate the effect of feedback point arrangement on the computational accuracy of three-dimensional UMI simulation by numerical analysis. The result of UMI simulation converges to the standard solution in the feedback domain and in some extent of the downstream region of the feedback domain. The computational accuracy is more improved as the increment of feedback point density, while the necessary computational time for convergence is unchanged.

  70. Bio-Fluids

    HAYASE Toshiyuki, FUNAMOTO Kenichi

    Fluids engineering conference ... 2004 201-201 2004/11/24

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2882

  71. Blood Flow Simulation Integrated with Ultrasonic Measurement 3^<rd> Report : Reproduction of Three-dimensional Blood Flow Field

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SAIJO Yoshifumi, YAMBE Tomoyuki

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 2004 (15) 113-114 2004/11/04

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

  72. 流体シミュレーションと融合した高度流体計測手法の開発 (第21回センシングフォーラム 資料--センシング技術の新たな展開と融合) -- (セッション1A1 高機能流速・流量センシング--流体計測部会企画OS)

    早瀬 敏幸, 船本 健一

    センシングフォ-ラム資料 21 3-6 2004/09/14

    Publisher: 〔計測自動制御学会〕

    ISSN:1343-7631

  73. Ultrasonic-Measurement-Integrated Simulation for Blood Flows 2^<nd> Report : Reproduction of Blood Flow in Aorta

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SHIRAI Atsushi, YAMBE Tomoyuki, SAIJO Yoshifumi

    2004 (16) 165-166 2004/01/21

    Publisher: The Japan Society of Mechanical Engineers

  74. B324 Study of Feedback Law for Ultrasonic-Measurement-Integrated Simulation of Blood Flow

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SHIRAI Atsushi, SAIJO Yoshifumi, YAMBE Tomoyuki

    2004 538-539 2004

    Publisher: 日本流体力学会

    More details Close

    In order to reproduce the blood velocity and pressure fields of the blood flow for an accurate diagnosis or treatment for serious circulatory diseases such as aortic aneurysms, we integrate color Doppler ultrasonography and numerical simulation. We term this method as Ultrasonic-Measurement-Integrated (UMI) simulation. In the UMI simulation, feedback signals are added to the governing equations to compensate for the difference between computation and measurement. In this paper, we investigate effects of feedback law of the UMI simulation, such as feedback formulae, number and arrangement of monitoring points and the direction of ultrasonic beam for acquisition of Doppler velocity data, on the accuracy of the UMI simulation and computational time. It is revealed that the result of UMI simulation in the feedback domain rapidly converges to the standard solution, even with inevitably incorrect upstream boundary conditions.

  75. Ultrasonic-Measurement-Integrated Simulation for Blood Flows : 1^<st> Report : Reduction of Error Originating from Upstream Boundary Condition

    FUNAMOTO Kenichi, HAYASE Toshiyuki, SHIRAI Atsushi, YAMBE Tomoyuki, SAIJO Yoshifumi

    Proceedings of the ... JSME Conference on Frontiers in Bioengineering 1 83-84 2003

    Publisher: The Japan Society of Mechanical Engineers

    ISSN:1348-2939

Show all ︎Show first 5

Books and Other Publications 1

  1. 高度物理刺激と生体応答

    Kenichi Funamoto

    Yokendo co. ltd. 2017/08

    ISBN: 9784842505626

    More details Close

    佐藤 岳彦, 大橋 俊朗, 川野 聡恭, 白樫 了 編著

Presentations 293

  1. ラグランジュ未定乗数法に基づく血管透過性の推定のためのデータ同化法(試行回数による誤差の収束性)

    茂 零音, 宮内 優, 竹内 伸太郎, 船本健一

    日本機械学会九州学生会第54回学生員卒業研究発表講演会 2023/03/03

  2. 低酸素環境下の乳がんスフェロイドの動態解析

    飯嶋 雄太, 船本 健一, 早瀬 元, 吉野 大輔

    日本機械学会第33回バイオエンジニアリング講演会 2022/12/17

  3. マイクロ流体デバイスの3次元培養系によるニワトリ胚肢芽間充織細胞の軟骨分化の観察

    柳田 翔平, 田村 宏治, 船本 健一

    日本機械学会第33回バイオフロンティア講演会 2022/12/17

  4. グルコース濃度と酸素濃度の変化に対する血管内皮細胞単層の応答の観察

    曽根 一輝, 廣瀬 理美, 吉野 大輔, 船本 健一

    日本機械学会第33回バイオフロンティア講演会 2022/12/18

  5. 細胞性粘菌の走気性の生化学的機構に関する研究

    廣瀬 理美, Jean-Paul Rieu, 船本 健一

    日本機械学会第33回バイオフロンティア講演会 2022/12/18

  6. Clarification of the aerotaxis of Dictyostelium discoideum in microfluidic device with oxygen controllability

    Satomi Hirose, Jean-Paul Rieu, Kenichi Funamoto

    Lab-on-a-Chip and Microfluidics World Congress 2022 2022/12/14

  7. Microfluidic platform for investigation of hypoxic responses of eukaryotic cells Invited

    Kenichi Funamoto

    The 6th FRIS-TFC Collaboration Event Workshop on Biosystems Design - From nanotechnology to microfluidics in biotechnology 2022/12/08

  8. Cancer cell migration under oxygen concentration gradients

    Satoshi Aratake, Zhouxing Su, Jean-Paul Rieu, Nicolas Aznar, Kenichi Funamoto

    ELyT Workshop 2022 2022/11/18

  9. Hypoxia triggers collective aerotactic spreading of eukaryotic cells

    Nasser Ghazi, Satomi Hirose, Jean-Paul Rieu, Christophe Anjard, Kenichi Funamoto

    ELyT Workshop 2022 2022/11/18

  10. Brain-targeted mechanical intervention using passive head motion can be antihypertensive

    Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Keisuke Shinohara, Takuya Kishi, Koji Tomiyasu, Toru Ogata, Hirotsugu Tsuchimochi, Shigeyoshi Saito, Kohzoh Yoshino, Kenichi Funamoto, Masahiro Shinohara, Motoshi Nagao, Yasuhiro Sawada

    Angiotensin Gordon Research Conference 2022 2023/01

  11. Three-dimensional culture of chick limb mesenchymal cells using microfluidic devices

    Shohei Yanagita, Koji Tamura, Kenichi Funamoto

    The 19th International Conference on Flow Dynamics (ICFD2022) 2022/11/09

  12. Observation of neutrophil-like HL-60 cell motility under oxygen gradient

    Masashi Tomita, Satomi Hirose, Kenichi Funamoto

    The 19th International Conference on Flow Dynamics (ICFD2022) 2022/11/10

  13. Microfluidic experiment about hypoxic responses of vascular endothelial cells under hyperglycemia

    Kazuki Sone, Satomi Hirose, Daisuke Yoshino, Kenichi Funamoto

    The 19th International Conference on Flow Dynamics (ICFD2022) 2022/11/10

  14. Data assimilation method for estimating membrane permeability based on the Lagrange multiplier method: Effect of signal-to-noise ratio on estimation accuracy

    Renon Shigeru, Suguru Miyauchi, Shintaro Takeuchi, Kenichi Funamoto

    The 22nd International Symposium on Advanced Fluid Information (AFI-2022) 2022/11/10

  15. Effects of pulsatile flow on endothelial permeability and cell motility

    Kenichi Funamoto, Eugenia Corvera Poiré

    The 22nd International Symposium on Advanced Fluid Information (AFI-2022) 2022/11/10

  16. Visualization of extracellular vesicles transport across brain microvasculature in a human 3D blood-brain barrier chip

    Yuka Sakamaki, Mai Inagaki, Momoko Sato, Miku Inai, Kenichi Funamoto, Masanori Tachikawa

    The 22nd International Symposium on Advanced Fluid Information (AFI-2022) 2022/11/10

  17. Numerical viscosity estimation considering inertial migration in plane Poiseuille suspension flow

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto

    The 22nd International Symposium on Advanced Fluid Information (AFI-2022) 2022/11/11

  18. Elucidation of a blood turbulence using electronic stethoscope

    Hikaru Dalton Yukimura, Jota Sasaki, Masatsugu Hirano, Kenichi Funamoto, Katsuhito Yamasaki

    The 22nd International Symposium on Advanced Fluid Information (AFI-2022) 2022/11/10

  19. マイクロ流体デバイス上に構築した3次元ヒト脳血管網の特性解析

    稲垣 舞, 佐藤 桃子, 船本 健一, 立川 正憲

    第37回日本薬物動態学会 2022/11/10

  20. マイクロ流体デバイスを用いた三次元ヒト血液脳関門の再構築と特性解析

    立川 正憲, 稲垣 舞, 酒巻 祐花, 佐藤 桃子, 船本 健一

    第44回神経組織培養研究会 2022/11/06

  21. マイクロ流体デバイスを用いた3次元ヒト脳血管網の再構築

    今井 健, 稲垣 舞, 佐藤 桃子, 船本 健一, 立川 正憲

    第61回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会 2022/11/05

  22. Brain-targeted mechanical intervention using passive head motion can have an antihypertensive effect

    Yasuhiro Sawada, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Keisuke Shinohara, Takuya Kishi, Ayumu Konno, Hirokazu Hirai, Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Toru Ogata, Hirotsugu Tsuchimochi, Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Kenichi Funamoto, Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao

    The Resuscitation Science Symposium 2022 2022/10/30

  23. Data assimilation method for estimating membrane permeability based on the Langrange multiplier method: formulation and fundamental examination

    Suguru Miyauchi, Shintaro Takeuchi, Kenichi Funamoto

    15th World Congress on Computational Mechanics (WCCM-XV) 8th Asian Pacific Congress on Computational Mechanics (APCOM-VIII) 2022

  24. 細胞性粘菌の走気性の特性の解析

    廣瀬 理美, Jean-Paul Rieu, 船本 健一

    日本機械学会第34回バイオエンジニアリング講演会 2022/06/25

  25. マイクロ流体デバイスを用いた三次元ヒト微小血管網モデルの構築と胎盤由来細胞外小胞の動態可視化

    酒巻 祐花, 稲垣 舞, 佐藤 桃子, 中野 瑛介, 船本 健一, 立川 正憲

    日本薬剤学会第37年会 2022/05/27

  26. マイクロ流体デバイスを用いた三次元ヒト脳微小血管網の構築

    佐藤 桃子, 稲垣 舞, 酒巻 祐花, 船本 健一, 立川 正憲

    日本薬学会第142年会 2022/03/26

  27. 生理学的レベルの酸素濃度勾配下におけるがん細胞の動態の観察

    荒武 聖, 吉野 大輔, 船本 健一

    日本機械学会第32回バイオフロンティア講演会 2022/01/13

  28. 低酸素負荷時のエンドサイトーシスによるVE-カドヘリンの内在化の評価

    曽根 一輝, 廣瀬 理美, 吉野 大輔, 船本 健一

    日本機械学会第32回バイオフロンティア講演会 2022/01/13

  29. 流れと低酸素負荷による血管内皮細胞の遊走速度の増加と低酸素誘導因子の核内移行

    菅原 竜志, 船本 健一

    日本機械学会第32回バイオフロンティア講演会 2022/01/12

  30. 繰り返し低酸素負荷による3次元微小血管網モデルの物質透過性の亢進

    二階堂 正隆, 大﨑 達哉, 船本健一

    日本機械学会第32回バイオフロンティア講演会 2022/01/12

  31. 大動脈二尖弁における左心室内血流場の変化に関する数値解析

    津田 晋吾, 宮内 優, 船本 健一

    日本機械学会第32回バイオフロンティア講演会 2022/01/12

  32. Migration characteristics of Dictyostelium;discoideum depending on oxygen environment

    Satomi Hirose, Jean-Paul Rieu, Kenichi Funamoto

    The 11th Asian-Pacific Conference on Biomechanics (AP Biomech 2021) 2021/12/04

  33. 酸素濃度依存性を有する血管内皮細胞の遊走におけるPAK1の活性化の評価

    曽根 一輝, 廣瀬 理美, 吉野 大輔, 船本 健一

    日本機械学会第33回バイオエンジニアリング講演会 2021/06/25

  34. 低酸素負荷による細胞性粘菌の運動性増進に関する評価

    廣瀬 理美, Jean-Paul Rieu, 船本 健一

    日本機械学会第33回バイオエンジニアリング講演会 2021/06/25

  35. マイクロ流体デバイスを用いた灌流性を有する三次元ヒト微小血管網の再構築

    酒巻 祐花, 稲垣 舞, 佐藤 桃子, 船本 健一, 立川 正憲

    日本薬剤学会第36年会 2021/05/13

  36. 酸素濃度制御下の3次元微小血管網モデルの物質透過性の評価

    二階堂 正隆, 大﨑 達哉, 船本 健一

    日本機械学会 第31回バイオフロンティア講演会 2020/12/12

  37. マイクロ流体デバイスによる酸素濃度勾配下の細胞性粘菌の運動解析

    廣瀬 理美, Jean-Paul Rieu, 船本 健一

    日本機械学会 第31回バイオフロンティア講演会 2020/12/13

  38. 乳腺がん細胞の低酸素感知と遊走速度の変化

    荒武 聖, 吉野 大輔, 船本 健一

    日本機械学会 第31回バイオフロンティア講演会 2020/12/13

  39. 低酸素負荷と流れ負荷時の血管内皮細胞の集団的遊走の評価

    高橋 直之, 吉野 大輔, 船本 健一

    日本機械学会 第31回バイオフロンティア講演会 2020/12/13

  40. Dynamics observation of vascular microenvironment by using microfluidic device with controllability of oxygen concentration Invited

    Kenichi Funamoto

    The 43rd Annual Meeting of the Molecular Biology Society of Japan 2020/12/03

  41. Observation and analysis of cellular dynamics by using organ-on-a-chip Invited

    Kenichi Funamoto

    2020/11/18

  42. 3D human blood-brain barrier chip for central nervous system drug development

    Momoko Sato, Yuuka Sakamaki, Mai Inagaki, Kenichi Funamoto, Masanori Tachikawa

    The 20th International Symposium on Advanced Fluid Information (AFI-2020) 2020/10/28

  43. Novel experimental-computational research framework for in-depth understanding of cancer mechanobiology

    Ryo Torii, Kenichi Funamoto

    The 20th International Symposium on Advanced Fluid Information (AFI-2020) 2020/10/28

  44. Mass transport analysis in a tumor microenvironment using finite element method

    Masataka Nikaido, Suguru Miyauchi, Kenichi Funamoto, Toshiyuki Hayase

    The 17th International Conference on Flow Dynamics (ICFD2020) 2020/10/30

  45. Measurement of oxygen;ension in microfluidic device by using oxygen-sensing film

    Naoyuki Takahashi, Satomi Hirose, Jean-Paul Rieu, Kenichi Funamoto

    The 17th International Conference on Flow Dynamics (ICFD2020) 2020/10/30

  46. Evaluation of Dictyostelium migration under oxygen concentration gradient

    Satomi Hirose, Jean-Paul Rieu, Kenichi Funamoto

    The 17th International Conference on Flow Dynamics (ICFD2020) 2020/10/30

  47. Changes of migration speed of breast cancer cells by oxygen sensing

    Satoshi Aratake, Daisuke Yoshino, Kenichi Funamoto

    The 17th International Conference on Flow Dynamics (ICFD2020) 2020/10/30

  48. Fundamental study of the blood flow field in the left ventricle with aortic regurgitation

    Ryuji Sugawara, Suguru Miyauchi, Toshiyuki Hayase, Kenichi Funamoto

    The 17th International Conference on Flow Dynamics (ICFD2020) 2020/10/30

  49. Experimental study on the radial particle distributions of neutrally buoyant spheres in a dilute suspension flow through a circular microchannel

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto, Miho Tanaka, Mitsuru Tanaka, Shigeru Murata, Suguru Miyauchi, Toshiyuki Hayase

    The 20th International Symposium on Advanced Fluid Information (AFI-2020) 2020/10/30

  50. Endothelial cell migration under oscillatory flow

    Kenichi Funamoto, Eugenia Corvera Poiré

    The 20th International Symposium on Advanced Fluid Information (AFI-2020) 2020/10/28

  51. Hypoxia triggers collective aerotactic migration in Dictyostelium discoideum

    Olivier Cochet-Escartin, Satomi Hirose, Kenichi. Funamoto, Christophe Anjard, Jean-Paul Rieu

    The 20th International Symposium on Advanced Fluid Information (AFI-2020) 2020/10/30

  52. 酸素に依存する細胞運動の機序解明を目的とした細胞性粘菌の走気性の観察

    廣瀬 理美, Jean-Paul Rieu, 船本 健一

    生体医工学シンポジウム2020 2020/09/19

  53. Reduced oxygen availability triggers aerotactic migration of Dictyostelium

    Jean-Paul Rieu, Kenichi Funamoto

    LyonSEN – ELyT Workshop 2020 2020/02/18

  54. Mechanism through which hydrostatic pressure promotes cell cycle in vascular endothelial cells

    Satomi Hirose, Kenichi Funamoto, Daisuke Yoshino

    2019/12/21

  55. Breast cancer cell migration under oxygen gradient

    Kenichi Funamoto, Rei Koens, Yugo Tabata, Daisuke Yoshino

    2019/12/21

  56. Oxygen-dependent migration of breast cancer cell is altered by cell-cell interaction with vascular endothelial cells

    Kenichi Funamoto, Rei Koens, Yugo Tabata, Daisuke Yoshino

    The 17th international conference on biomedical engineering (ICBME 2019) 2019/12/11

  57. Increased hydrostatic pressure affects vascular endothelial cell behavior

    Satomi Hirose, Kenichi Funamoto, Daisuke Yoshino

    The 17th international conference on biomedical engineering (ICBME 2019) 2019/12/09

  58. Controlled oxygen tension regulates vascular endothelial cell migration through p21-activated kinase signaling

    Yugo Tabata, Naoyuki Takahashi, Daisuke Yoshino, Kenichi Funamoto

    The 17th international conference on biomedical engineering (ICBME 2019) 2019/12/10

  59. Vascular endothelial responses to fluid flow under hypoxic condition

    Naoyuki Takahashi, Yugo Tabata, Satomi Hirose, Kiyoe Funamoto, Daisuke Yoshino, Kenichi Funamoto

    The 17th international conference on biomedical engineering (ICBME 2019) 2019/12/11

  60. Microfluidic device for reproduction of in vivo microenvironment with three-dimensional vascular structure Invited

    Kenichi Funamoto

    The 34th Annual Meeting of the Japanese Society for the Study of Xenobiotics 2019/12/10

  61. Investigation of oxygen-dependent vascular endothelial cell migration using microfluidic device

    Yugo Tabata, Naoyuki Takahashi, Daisuke Yoshino, Kenichi Funamoto

    The 16th International Conference on Flow Dynamics (ICFD2019) 2019/11/08

  62. A method to control oxygen tension in microfluidic device for reproducing vascular microenvironment

    Naoyuki Takahashi, Yugo Tabata, Daisuke Yoshino, Kenichi Funamoto

    The 16th International Conference on Flow Dynamics (ICFD2019) 2019/11/08

  63. Measurement of particle concentration profiles of a dilute suspension in different Reynolds number conditions

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto, Mitsuru Tanaka, Shigeru Murata, Suguru Miyauchi, Toshiyuki Hayase

    The 19th International Symposium on Advanced Fluid Information (AFI-2019) 2019/11/07

  64. 高血圧様の静水圧刺激に対する血管内皮細胞の応答

    廣瀬 理美, 船本 健一, 吉野 大輔

    日本機械学会第30回バイオフロンティア講演会 2019/07/20

  65. 血管内低酸素環境模擬チップによる血管内皮細胞の形態変化の評価

    高橋 直之, 田端 優吾, 廣瀬 理美, 船本 聖絵, 吉野 大輔, 船本 健一

    日本機械学会第30回バイオフロンティア講演会 2019/07/19

  66. 低酸素負荷による血管内皮細胞の接着結合と遊走の変化

    田端 優吾, 吉野 大輔, 船本 聖絵, 船本 健一

    日本機械学会第30回バイオフロンティア講演会 2019/07/19

  67. がん微小環境チップによる酸素濃度制御下の細胞外マトリクスの変化の観察

    船本 健一, 吉野 大輔

    日本機械学会第31回バイオエンジニアリング講演会 2018/12/15

  68. Breast cancer cell migration under controlled oxygen tensions International-presentation

    Rei Koens, Yugo Tabata, Daisuke Yoshino, Kenichi Funamoto

    The 15th International Conference on Flow Dynamics (ICFD2018) 2018/11/08

  69. Influence of hypoxic environment on vascular endothelial cell migration International-presentation

    Yugo Tabata, Daisuke Yoshino, Kiyoe Funamoto, Rei Koens, Kenichi Funamoto

    The 15th International Conference on Flow Dynamics (ICFD2018) 2018/11/08

  70. Numerical and experimental studies on non-Newtonian rheology of a suspension International-presentation

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto, Suguru Miyauchi, Toshiyuki Hayase

    The 18th International Symposium on Advanced Fluid Information (AFI-2018) 2018/11/08

  71. マイクロ流体デバイスを用いた酸素制御下の血管内皮細胞単層の遊走の評価

    田端 優吾, 吉野 大輔, 船本 聖絵, コーエンズ 礼, 船本 健一

    日本機械学会第29回バイオフロンティア講演会 2018/10/25

  72. 乳腺がん細胞の遊走と増殖の酸素依存性

    コーエンズ 礼, 田端 優吾, 吉野 大輔, Roger D. Kamm, 船本 健一

    日本機械学会第29回バイオフロンティア講演会 2018/10/25

  73. 地球の裏側で Invited

    船本 健一

    日本機械学会第29回バイオフロンティア講演会 2018/10/24

  74. A loss of vascular endothelial barrier function by hypoxic exposure International-presentation

    Kenichi Funamoto, Yugo Tabata, Rei Koens, Daisuke Yoshino

    World Congress of Biomechanics 2018 (WCB2018) 2018/07/09

  75. 低酸素ストレスに対する血管内皮細胞単層の細胞間結合の初期応答

    船本 健一, 吉野 大輔, 中山 勝文

    日本機械学会第30回バイオエンジニアリング講演会 2017/12/14

  76. Microfluidic device for co-culture under controlled oxygen tension International-presentation

    Kenichi Funamoto, Daisuke Yoshino

    The 3rd International Symposium on Mechanobiology (ISMB2017) 2017/12/11

  77. Estimation of viscosity profiles of semidilute suspensions by computational and experimental studies International-presentation

    Misa Kawaguchi, Tomohiro Fukui, Kenichi Funamoto, Toshiyuki Hayase

    The 17th International Symposium on Advanced Fluid Information (AFI-2017) 2017/11/02

  78. 生体内低酸素環境を再現するマイクロ流体デバイスの酸素濃度制御性能の向上

    コーエンズ 礼, 田端 優吾, 吉野 大輔, Roger D. Kamm, 船本 健一

    日本機械学会第28回バイオフロンティア講演会 2017/10/29

  79. Microfluidic experiments of cellular responses to hypoxia International-presentation Invited

    臺灣人才躍昇計畫 2017系列講座(三) 2017/09/08

  80. The neurophysiology of brain sparing International-presentation

    Clarissa, L. Velayo, Takuya Ito, YuPeng Dong, Kenichi Funamono, Kiyoe Funamono, Keita Iida, Miyuki Endo, Rika Sugibayashi, Nobuo Yaegashi, Yoshitaka Kimura

    The 44th Annual Meeting Fetal and Neonatal Physiological Society 2017/09/04

  81. Excess retinoic acid to pregnant mouse increases the risk of Long QT syndrome for the fetal heart International-presentation

    Kiyoe Funamoto, Motoyoshi Kawataki, Kana Nakanishi, Chihiro Yoshida, Kenichi Funamoto, YuPeng Dong, Takuya Ito, Naoaki Sato, Yoshitaka Kimura

    The Joint Meeting of the German and Japanese Societies of Developmental Biologists 2017/03/17

  82. 低酸素負荷による血管内皮細胞単層の物質透過性と接着結合の変化

    船本 健一, 吉野 大輔, 松原 健人, 船本 聖絵, 木村 芳孝, Roger D. Kamm

    日本機械学会第29回バイオエンジニアリング講演会 2017/01/20

  83. Microfluidic device for investigation of cellular responses to heterogeneity of oxygen tension International-presentation

    Kenichi Funamoto, Roger D. Kamm

    The 16th International Conference on Biomedical Engineering (ICBME 2016) 2016/12/07

  84. Microfluidic experiments of cellular responses to hypoxia International-presentation

    Kenichi Funamoto

    The 13th International Conference on Flow Dynamics (ICFD2016) 2016/10/11

  85. Preliminary study for fluid dynamic effects of upstream bifurcation and bend on blood flow simulation in cerebral aneurysm International-presentation

    Daichi Suzuki, Kenichi Funamoto, Shin-ichiro Sugiyama, Toshiyuki Hayase, Suguru Miyauchi, Teiji Tominaga

    The 16th International Conference on Biomedical Engineering (ICBME 2016) 2016/10/11

  86. Analysis of an autonomic nervous system of mouse fetus with congenital heart defect International-presentation

    Kiyoe Funamoto, Rika Sugibayashi, Kenichi Funamoto, Kana Nakanishi, Takuya Ito, Motoyoshi Kawataki, Toshiyuki Hayase, Yoshitaka Kimura

    The 16th International Symposium on Advanced Fluid Information (AFI-2016) 2016/10/11

  87. Computational and experimental studies on the blood cells behavior in microcirculation International-presentation

    Tomohiro Fukui, Misa Kawaguchi, Atsuhide Kitagawa, Kenichi Funamoto, Toshiyuki Hayase

    The 16th International Symposium on Advanced Fluid Information (AFI-2016) 2016/10/11

  88. 拍動に伴う血管変形を考慮した2次元超音波計測融合血流解析システム

    原田 大輔, 早瀬 敏幸, 宮内 優, 井上 浩介, 門脇 弘子, 船本 健一, 島崎 正, 地挽 隆夫, 見山 広二

    日本超音波医学会第89回学術集会 2016/05/27

  89. Investigation of permeability of endothelial cell monolayer by using microfluidic device with controllability of oxygen tension

    日本機械学会第28回バイオエンジニアリング講演会 2016/01/10

  90. 静水圧負荷による一過性のERK活性を介した血管新生の促進

    吉野 大輔, 船本 健一, 佐藤 正明, Lim Chwee Teck

    日本機械学会第28回バイオエンジニアリング講演会 2016/01/09

  91. Study of Permeability Changes of Endothelial Cell Monolayer Exposed to Hypoxia International-presentation

    Kento Matsubara, Kenichi Funamoto, Ioannis K. Zervantonakis, Kiyoe Funamoto, Takuya Ito, Yoshitaka Kimura, Toshiyuki Hayase, Roger D. Kamm

    The 12th International Conference on Flow Dynamics (ICFD2015) 2015/10/29

  92. Determination of Optimum Feedback Gain of Two-Dimensional Ultrasonic-Measurement-Integrated Blood Flow Analysis System International-presentation

    Hiroko Kadowaki, Toshiyuki Hayase, Kenichi Funamoto, Suguru Miyauchi, Kosuke Inoue, Tadashi Shimazaki, Takao Jibiki, Koji Miyama

    The 12th International Conference on Flow Dynamics (ICFD2015) 2015/10/29

  93. Fundamental Study of Interaction between Erythrocyte and Endothelial Cell under Inclined Centrifugal Force (Physical Explanation of Interaction Model) International-presentation

    Akira Yatsuyagagi, Toshiyuki Hayase, Suguru Miyauchi, Kenichi Funamoto, Kosuke Inoue, Atsushi Shirai, Luca Brandt

    The 12th International Conference on Flow Dynamics (ICFD2015) 2015/10/28

  94. Numerical Analysis of Ultrasound Scattering Property of Medium Mimicking Blood (Influence of Ultrasound Frequency) International-presentation

    Yoshiki Chiba, Toshiyuki Hayase, Suguru Miyauchi, Kenichi Funamoto

    The 12th International Conference on Flow Dynamics (ICFD2015) 2015/10/28

  95. Cardiac Evaluation of Fetal Mice by ECG and Ultrasound International-presentation

    Rika Sugibayashi, Takuya Ito, Kenichi Funamoto, Toshiyuki Hayase, Yoshitaka Kimura

    The 15th International Symposium on Advanced Fluid Information (AFI-2015) 2015/10/28

  96. Investigation of Inlet Position in Hemodynamic Analysis of a Cerebral Aneurysm International-presentation

    Daichi Suzuki, Kenichi Funamoto, Shin-ichiro Sugiyama, Toshiyuki Hayase, Suguru Miyauchi, Teiji Tominaga

    The 12th International Conference on Flow Dynamics (ICFD2015) 2015/10/27

  97. マイクロ流体デバイスによる低酸素下の血管内皮細胞の物質透過性の計測

    松原 健人, 船本 健一, Ioannis Zervantonakis, 船本 聖絵, 伊藤 拓哉, 正宗 淳, 木村 芳孝, 早瀬 敏幸, Roger Kamm

    日本機械学会第26回バイオフロンティア講演会 2015/10/02

  98. 2次元超音波計測融合血流解析における上流端流速推定手法の実血管形状による検証

    門脇 弘子, 早瀬 敏幸, 船本 健一, 宮内 優, 谷口 信行

    日本流体力学会年会2015 2015/09/27

  99. Effect of Speckle Noise in Ultrasonic Measurement on Two-Dimensional Ultrasonic-Measurement-Integrated Blood Flow Analysis International-presentation

    Hiroko Kadowaki, Toshiyuki Hayase, Kenichi Funamoto, Suguru Miyauchi, Kosuke Inoue, Tadashi Shimazaki, Takao Jibiki, Koji Miyama

    The International Conference on Biotechnology and Bioengineering 2015/07/14

  100. 2次元超音波計測融合血流解析システムにおける最適フィードバックゲインの決定

    門脇 弘子, 早瀬 敏幸, 船本 健一, 井上 浩介, 島崎 正, 地挽 隆夫, 見山 広二

    日本超音波医学会第88回学術集会 2015/05/22

  101. Measurements of Blood Flow and Blood Pressure under Different Indoor Temperature and Body Postural Conditions, and Development of a New Human Simulation Model International-presentation

    Hiroto Sakamoto, Yuki Chiba, Toshiyuki Hayase, Kenichi Funamoto, Yoshifumi Saijo, Tomonobu Goto

    Healthy Buildings Europe 2015 2015/05/19

  102. 2次元超音波計測融合血流解析システムにおける超音波計測ノイズと解析精度に関する実験的検討

    門脇 弘子, 早瀬 敏幸, 船本 健一, 井上 浩介, 島崎 正, 地挽 隆夫, 見山 広二

    流体科学研究所・統計数理研究所 合同ワークショップ 2015/03/26

  103. 2次元超音波計測融合血流解析システムの有用性の検証

    船本 健一, 早瀬 敏幸, 曾根 周作, 門脇 弘子, 島崎 正, 地挽 隆夫, 見山 広二

    流体科学研究所・統計数理研究所 合同ワークショップ 2015/03/26

  104. MR画像データに基づく左心室内血流場の数値解析(非定常3D心室モデルの構築)

    山田 智美, 早瀬 敏幸, 船本 健一

    日本機械学会東北支部第50期総会・講演会 2015/03/13

  105. Analysis of High Reynolds Number Flow Behind a Square Cylinder by Hybrid Wind Tunnel (Reproduction of Turbulent Velocity Fluctuations) International-presentation

    Jumpei Okutani, Toshiyuki Hayase, Kosuke Inoue, Shusaku Sone, Kenichi Funamoto

    2015 Annual ELyT Workshop 2015/02/19

  106. 脳動脈瘤内の血流の数値解析における上流端の設定法の検討

    鈴木 大地, 船本 健一, 杉山 慎一郎, 早瀬 敏幸, 冨永 悌二

    日本機械学会第27回バイオエンジニアリング講演会 2015/01/10

  107. 3次元非定常の超音波計測融合血流解析における圧力場の再現に関する検討

    船本 健一, 早瀬 敏幸

    日本機械学会第27回バイオエンジニアリング講演会 2015/01/09

  108. 酸素濃度依存の細胞応答観察のためのマイクロ流体デバイスの開発

    藤高 直也, 福島 修一郎, 船本 健一, 橋本 守, 荒木 勉

    日本機械学会第27回バイオエンジニアリング講演会 2015/01/09

  109. 低酸素マイクロ流体デバイスを用いた血管内皮細胞単層の物質透過性の評価

    松原 健人, 船本 健一, Ioannis Zervantonakis, 船本 聖絵, 伊藤 拓哉, 正宗 淳, 木村 芳孝, 早瀬 敏幸, Roger Kamm

    日本機械学会第27回バイオエンジニアリング講演会 2015/01/09

  110. 傾斜遠心力場における赤血球と内皮細胞との力学的相互作用の解明(相互作用モデルの物理的解釈)

    八柳 暁, 早瀬 敏幸, 船本 健一, 井上 浩介, 白井 敦

    日本機械学会第27回バイオエンジニアリング講演会 2015/01/09

  111. 傾斜遠心顕微鏡による内皮細胞上の赤血球の摩擦特性の計測(傾斜遠心力の垂直成分の影響)

    井上 浩介, 早瀬 敏幸, 船本 健一, 白井 敦

    日本機械学会第27回バイオエンジニアリング講演会 2015/01/09

  112. 希釈した血液を模擬した媒質における超音波の散乱特性に関する数値解析(超音波の周波数の影響)

    千葉 吉規, 早瀬 敏幸, 船本 健一

    日本機械学会第27回バイオエンジニアリング講演会 2015/01/09

  113. 2次元超音波計測融合シミュレーションシステムにおける解析精度の実験的検証

    門脇 弘子, 早瀬 敏幸, 船本 健一, 井上 浩介, 島崎 正, 地挽 隆夫, 見山 広二

    日本機械学会第27回バイオエンジニアリング講演会 2015/01/09

  114. 超音波計測と計測融合シミュレーションによる血圧場と血流場の同時解析システム

    曾根 周作, 早瀬 敏幸, 船本 健一, 白井 敦

    日本機械学会第27回バイオエンジニアリング講演会 2015/01/09

  115. 2次元超音波計測融合血流解析システムによる頸動脈内の血流解析

    第14回日本流体力学会中四国・九州支部講演会 2014/12/14

  116. 2次元超音波計測融合シミュレーションによる頸動脈分岐部の血流解析

    計測自動制御学会東北支部50周年記念学術講演会 2014/12/11

  117. 酸素濃度制御マイクロ流体デバイスを用いた細胞実験

    第18回酸素ダイナミクス研究会 2014/11/29

  118. Analysis of High Reynolds Number Flow behind a Square Cylinder by Hybrid Wind Tunnel (Improvement of Critical Gain of Instability for Pressure Feedback) International-presentation

    The 11th International Conference on Flow Dynamics (ICFD2014) 2014/10/10

  119. Application of MR-Measurement-Integrated Hemodynamic Simulation to Cerebrovascular Diseases International-presentation

    The 14th International Symposium on Advanced Fluid Information (AFI-2014) 2014/10/09

  120. Cardiac Evaluation of Fetal Mice by ECG and Ultrasound International-presentation

    The 14th International Symposium on Advanced Fluid Information (AFI-2014) 2014/10/09

  121. Elucidation of Mechanisms of the Frictional Characteristics of Erythrocytes under Inclined Centrifugal Force International-presentation

    The 14th International Symposium on Advanced Fluid Information (AFI-2014) 2014/10/09

  122. Evaluation of Permeability of Endothelial Cell Monolayer under Controlled Oxygen Tension International-presentation

    The 14th International Symposium on Advanced Fluid Information (AFI-2014) 2014/10/09

  123. Investigation on Advanced Medical Ultrasound Imaging Technology International-presentation

    The 14th International Symposium on Advanced Fluid Information (AFI-2014) 2014/10/09

  124. Observation of Hypoxia Cellular Response by Using Microfluidic Devices International-presentation

    The 14th International Symposium on Advanced Fluid Information (AFI-2014) 2014/10/09

  125. Fundamental Study of Interaction between Erythrocyte and Endothelial Cell under Inclined Centrifugal Force (Reproduction of Friction Characteristics by Numerical Analysis Using Simple Interaction Model) International-presentation

    Eleventh International Conference on Flow Dynamics (ICFD2014) 2014/10/08

  126. 光電容積脈波計測と超音波計測融合シミュレーションによる頸動脈内の血圧と血流場の同時解析

    日本流体力学会年会2014 2014/09/17

  127. 超音波計測融合シミュレーションにおける血流量推定手法の有効性の検証

    日本流体力学会年会2014 2014/09/17

  128. “Sonocytometry” - Novel Diagnostic Method of Ultrasonic Differentiation of Cells in Blood Flow International-presentation

    The 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC’14) 2014/08

  129. Simultaneous Analysis System for Blood Pressure and Flow Using Ultrasonic-Measurement-Integrated Simulation International-presentation

    7th World Congress of Biomechanics 2014/07/11

  130. Observation of Extracellular Collagen Remodeling by Second-Harmonic-Generation Microscopy International-presentation

    The 7th World Congress of Biomechanics 2014/07/06

  131. Twinkling Sign機序解明のための超音波照射による微粒子挙動の光学観察

    日本超音波医学会第87回学術集会 2014/05/10

  132. ソノサイトメトリーの基盤技術の検討

    日本超音波医学会第87回学術集会 2014/05/09

  133. 光電脈波計測と超音波計測融合解析による血圧血流同時計測システムによるWI解析

    日本超音波医学会第87回学術集会 2014/05/09

  134. 超音波計測融合シミュレーションにおける血流量推定精度の向上

    日本超音波医学会第87回学術集会 2014/05/09

  135. ハイブリッド風洞による角柱後流の高レイノルズ数流れ解析(圧力フィードバックにおける不安定化臨界ゲインの改善)

    日本機械学会東北支部第49期総会・講演会 2014/03/14

  136. 傾斜遠心力場における赤血球と内皮細胞との力学的相互作用の解明(赤血球とグリコカリックスの相互作用モデルを用いた数値解析による摩擦特性の再現)

    日本機械学会東北支部第49期総会・講演会 2014/03/14

  137. 光電脈波計測と超音波計測融合シミュレーションによる血圧と血流の同時計測システムの開発(開発システムによるWave Intensityの評価)

    日本機械学会第26回バイオエンジニアリング講演会 2014/01/12

  138. 小動物用超音波計測連成解析システムによる3次元血管形状抽出と血流解析の精度検証

    日本機械学会第26回バイオエンジニアリング講演会 2014/01/12

  139. 総頸動脈内血流の2次元超音波計測融合シミュレーションにおける2次元断面流量の軸方向変化の影響

    日本機械学会第26回バイオエンジニアリング講演会 2014/01/12

  140. 超音波計測融合シミュレーションにおける血流量推定の評価関数に関する考察

    日本機械学会第26回バイオエンジニアリング講演会 2014/01/12

  141. 脳動脈瘤の壁面性状と血行力学パラメータの比較

    日本機械学会第26回バイオエンジニアリング講演会 2014/01/11

  142. 微小流体デバイス内における血管新生過程の基質再構築

    日本機械学会第26回バイオエンジニアリング講演会 2014/01/11

  143. Effects of Time-Varying Feedback Signals on Pressure Field in Ultrasonic-Measurement-Integrated Simulation of Pulsatile Blood Flow International-presentation

    The 3rd International Conference on Computational and Mathematical Biomedical Engineering (CMBE13) 2013/12/17

  144. 低酸素マイクロ流体デバイスの開発

    第11回がんとハイポキシア研究会 2013/12/13

  145. Evaluation by High-Frequency Ultrasound B-Mode Imaging of Cerebral Hemorrhage in Mouse Fetal Brain Resulting from Ischemia/Reperfusion International-presentation

    The 15th International Conference on Biomedical Engineering (ICBME 2013) 2013/12/07

  146. Validation of blood vessel geometry reconstruction and of blood flow analysis by ultrasonic-measurement-integrated flow-structure interaction simulation system for small animals International-presentation

    The 15th International Conference on Biomedical Engineering 2013/12/07

  147. Comparison of Hemodynamic Parameters and Wall Condition of Cerebral Aneurysm International-presentation

    The 15th International Conference on Biomedical Engineering (ICBME 2013) 2013/12/06

  148. Hemodynamic Analysis of Intracranial Aneurysms with Atherosclerosis International-presentation

    The 15th International Conference on Biomedical Engineering (ICBME 2013) 2013/12/06

  149. The Effect of Axial Variation of the Plane Flow Rate on Two-Dimensional Ultrasonic-Measurement-Integrated Simulation of Blood Flow in a Common Carotid Artery International-presentation

    The 15th International Conference on Biomedical Engineering (ICBME 2013) 2013/12/06

  150. Cardiac Evaluation of Fetal Mice by ECG and Ultrasound International-presentation

    The 13th International Symposium on Advanced Fluid Information (AFI-2013) 2013/11/26

  151. Development of a Microfluidic Device for a Three-Dimensional Cell Culture under a Controlled Hypoxic Environment International-presentation

    The 13th International Symposium on Advanced Fluid Information (AFI-2013) 2013/11/26

  152. Effects of Temporal and Spatial Oxygen Heterogeneity on Cell Processes International-presentation

    The 13th International Symposium on Advanced Fluid Information (AFI-2013) 2013/11/26

  153. Evaluation of Intracranial Aneurysm Rupture Using MR-Measurement-Integrated Simulation International-presentation

    The 13th International Symposium on Advanced Fluid Information (AFI-2013) 2013/11/26

  154. In-Depth Investigation of Twinkling Sign: Optical Observation of Ultrasound Radiation Force Driven Oscillation of Glass Particle International-presentation

    The 13th International Symposium on Advanced Fluid Information (AFI-2013) 2013/11/26

  155. Numerical Simulation of Ultrasound Imaging for Detection of Microcalcification in Soft Tissue International-presentation

    The 13th International Symposium on Advanced Fluid Information (AFI-2013) 2013/11/26

  156. Observation of Hypoxia Cellular Response by Using Microfluidic Devices International-presentation

    The 13th International Symposium on Advanced Fluid Information (AFI-2013) 2013/11/26

  157. Improvement of Simultaneous Analysis System for Blood Pressure and Flow Velocity Using Photoplethymography and Ultrasonic-Measurement-Integrated Simulation International-presentation

    7th East Asian Consortium on Biomedical Engineering 2013/11/18

  158. Consideration on the Evaluation Function of Blood Flow Rate Estimation in Ultrasonic-Measurement-Integrated Simulation International-presentation

    7th East Asian Consortium on Biomedical Engineering 2013/11/18

  159. Ultrasonic-Measurement-Integrated Simulation of Complex Blood Flow International-presentation

    The International Workshop on Flow Dynamics Related to Energy, Aerospace and Material Science 2013/09/11

  160. マイクロ流体デバイスを用いた細胞の低酸素応答の観察

    日本機械学会2013年度年次大会 2013/09/10

  161. Fundamental Study on Micro Calcification Detection Using Twinkling Sign (TS): The Effect of Stiffness of Surrounding Tissue on the Appearance of TS International-presentation

    The 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC’13) 2013/07/04

  162. Simultaneous Analysis System for Blood Pressure and Flow Using Photoplethysmography and Ultrasonic-Measurement-Integrated Simulation International-presentation

    35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC’13) 2013/07/04

  163. Frictional Characteristics of Erythrocytes on Endothelia-Cultured or Material-Coated Glass Plates Subject to Inclined Centrifugal Forces International-presentation

    8th International Conference on Multiphase Flow (ICMF 2013) 2013/05/29

  164. 超音波計測融合シミュレーションによる生体内の血液粘度の推定

    日本超音波医学会第86回学術集会 2013/05/24

  165. 軟組織内微細石灰化Twinkling Signに関する実験的研究:粒子径,粒子種類が与える影響

    日本超音波医学会第86回学術集会 2013/05/24

  166. 超音波Mモードイメージングと光電容積脈波センシングによる脈波同定の比較

    日本超音波医学会第86回学術集会 2013/05/24

  167. 脳動脈瘤内の3次元血流解析とMRI計測の比較

    日本機械学会第25回バイオエンジニアリング講演会 2013/01/11

  168. マイクロ流体デバイスを用いた低酸素下におけるがん細胞の挙動の観察

    日本機械学会第25回バイオエンジニアリング講演会 2013/01/11

  169. 頸動脈内の3次元血流場に対する2次元超音波計測融合シミュレーションの解析精度の検証

    日本機械学会第25回バイオエンジニアリング講演会 2013/01/10

  170. 超音波計測融合シミュレーションによる血液粘度の推定

    日本機械学会第25回バイオエンジニアリング講演会 2013/01/10

  171. 血管内圧による狭窄部の形状変化と血流分布の関連性

    日本機械学会第25回バイオエンジニアリング講演会 2013/01/10

  172. 小動物用超音波計測連成解析システムによるマウスの頸動脈の血流解析

    日本機械学会第25回バイオエンジニアリング講演会 2013/01/10

  173. 酸素濃度制御下の細胞実験のためのマイクロ流体デバイスの設計

    COMSOL Conference Tokyo 2012 2012/11/22

  174. 低酸素下細胞実験のためのマイクロ流体デバイスの開発

    日本機械学会第4回マイクロ・ナノ工学シンポジウム 2012/10/23

  175. 脳動脈瘤破裂点の流体力学的特徴: コンピュータ流体解析による検討

    日本脳神経外科学会第71回学術総会 2012/10/19

  176. 小動物用超音波計測連成解析システムにおける血管形状抽出の高精度化

    坂西 山河, 早瀬 敏幸, 船本 健一, 曾根 周作

    日本機械学会第23回バイオフロンティア講演会 2012/10/06

  177. 2次元超音波計測融合シミュレーションによる頸動脈分岐部の血流解析

    日本機械学会第23回バイオフロンティア講演会 2012/10/05

  178. Neumerical and Experimental Study of Flow in Stenosis models with Several Mechanical Properties International-presentation

    The 9th International Conference on Flow Dynamics (ICFD2012) 2012/09/21

  179. Rheological Analysis of the Mechanism of Fetal Brain Hemorrhage International-presentation

    The 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 2012/09/20

  180. Ultrasound Radiation Force Driven B-Flow Twinkling Sign for Microcalcification Detection International-presentation

    The 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 2012/09/20

  181. Moving on to the Small World: Microfluidic Cell Culture International-presentation

    The 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 2012/09/20

  182. Magnetic Resonance Fluid Dynamics for Intracranial Aneurysms International-presentation

    The 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 2012/09/20

  183. Observation of Hypoxia Cellular Response by Using Microfluidic Devices International-presentation

    The 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 2012/09/20

  184. Computational Simulation to Create Low Oxygen Tension in a Microfluidic Device for Cell Culture International-presentation

    The 12th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2012) 2012/09/20

  185. Numerical Analysis of Levitation Mechanism of Red Blood Cell in Inclined Centrifuge Microscope - Effect of Asymmetric Cell Shape on the Motion International-presentation

    Takashi Oshibe, Toshiyuki Hayase, Kenichi Funamoto, Atsushi Shirai

    The 9th International Conference on Flow Dynamics (ICFD2012) 2012/09/20

  186. Oxygen Tension Control in a Microfluidic Device for Cell Culture International-presentation

    The 9th International Conference on Flow Dynamics (ICFD2012) 2012/09/19

  187. Intra-left Atrial Flow International-presentation

    The 9th International Conference on Flow Dynamics (ICFD2012) 2012/09/19

  188. A Novel Approach to Understanding Perinatal Hypoxic-Ischemia International-presentation

    The 11th World Congress in Fetal Medicine 2012/06/24

  189. 視覚協創学(9):視覚協創による血流動態のリアライゼーション

    早瀬 敏幸, 船本 健一, 曾根 周作, 坂西 山河

    第17回計算工学講演会 2012/05/30

  190. 軟組織内微細石灰化のB-Flow“Twinkling Sign”発生メカニズムに関するin vitro研究

    日本超音波医学会第85回学術集会 2012/05/26

  191. 2次元超音波計測融合シミュレーションによる頸動脈分岐部の非定常血流量推定

    日本超音波医学会第85回学術集会 2012/05/25

  192. Oxygen sensors for microfluidic 3D cell cultures International-presentation

    EUROPT(R)ODE XI Conference on Optical Chemical Sensors and Biosensors 2012/04/02

  193. 頸動脈分岐部内血流の2次元超音波計測融合シミュレーション

    日本機械学会第24回バイオエンジニアリング講演会 2012/01/08

  194. 傾斜遠心顕微鏡下での赤血球の浮上機構に関する数値解析 第2報:剛体赤血球モデルによる摩擦特性の再現性の検討

    日本機械学会第24回バイオエンジニアリング講演会 2012/01/08

  195. Flow Analysis of Rupture Point in Cerebral Aneurysms: Computational Fluid Dynamics Study International-presentation

    The 5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering 2011/12/13

  196. Numerical Analysis of the Effect of Angle of Attack on a Red Blood Cell Moving in an Inclined Centrifuge Microscope International-presentation

    The 5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering 2011/12/13

  197. Two-Dimensional Ultrasonic-Measurement-Integrated Simulation of Blood Flow in Bifurcation of Carotid Artery International-presentation

    The 5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering 2011/12/13

  198. Comparison between Ultrasonic-Measurement-Integrated Simulation and Ordinary Simulation with Measured Upstream Velocity Condition International-presentation

    The 8th International Conference on Flow Dynamics 2011/11/10

  199. Computational Simulation of Blood Flow in Intracranial Aneurysms under Patient-Specific Pulsatile Inlet Condition International-presentation

    The 11th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2011) 2011/11/10

  200. Detection of Microcalcification in Soft Tissue Employing B-Flow ”Twinkling” Sign International-presentation

    The 11th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2011) 2011/11/10

  201. Left Atrial Vortex International-presentation

    The 11th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2011) 2011/11/10

  202. Rheological Analysis of the Mechanism of Fetal Brain Hemorrhage International-presentation

    The 11th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2011) 2011/11/10

  203. 微細石灰化を含んだ乳房組織を模擬したPVA phantomの実験的研究

    第49回人工臓器学会 2011/11

  204. 傾斜遠心顕微鏡下での赤血球浮上機構に関する数値解析

    日本機械学会第22回バイオフロンティア講演会 2011/10/07

  205. Hemodynamic Changes in the Left Atrium due to Atrial Fibrillation International-presentation

    The ASME 2011 Summer Bioengineering Conference 2011/06/24

  206. “Twinkling Sign”を応用した軟組織内微細石灰化検出に関する基礎的研究

    日本超音波医学会第84回学術集会 2011/05/28

  207. 超音波計測融合シミュレーションと計測による上流速度を与えたシミュレーションの比較

    日本超音波医学会第84回学術集会 2011/05/28

  208. Integration of Ultrasound Color Doppler Imaging and Numerical Simulation International-presentation

    The 2nd International Conference on Computational and Mathematical Biomedical Engineering (CMBE11) 2011/04/01

  209. 頚動脈モデルを用いた超音波計測融合血流シミュレーションの検証実験

    第23回バイオエンジニアリング講演会 2011/01/08

  210. Blood Flow Analysis in the Left Atrium with/without Atrial Fibrillation International-presentation

    The 4th East Asian Pacific Student Workshop on Nano-Biomedical Engineering 2010/12/16

  211. Experimental Validation of Ultrasonic-Measurement-Integrated Blood Flow Simulation Using Carotid Artery Models International-presentation

    The 4th East Asian Pacific Student Workshop on Nano-Biomedical Engineering 2010/12/15

  212. MR-Measurement-Integrated Simulation for Reproduction of Blood Flow in Cerebral Aneurysm International-presentation

    Swiss/Japan International Seminar on Medical Engineering Based on Vessel Biology 2010/11/15

  213. 小動物用超音波計測融合シミュレーションシステムの開発(検証実験)

    第21回バイオフロンティア講演会 2010/11/13

  214. 超音波計測融合シミュレーションによる頚動脈内の壁せん断応力の評価

    第21回バイオフロンティア講演会 2010/11/13

  215. 心房細動に関する左心房内血流解析

    第21回バイオフロンティア講演会 2010/11/12

  216. Computer Simulation Predicts the Convective Drug Distribution in the Primate Brainstem International-presentation

    The tenth International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2010) 2010/11/02

  217. Consideration for Simulations of Infusion in Realistic Animal Brain Geometries International-presentation

    The tenth International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2010) 2010/11/02

  218. Detection of Microcalcification in Soft Tissue International-presentation

    The tenth International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2010) 2010/11/02

  219. Evaluation of Wall Shear Stress on Carotid Artery with Ultrasonic-Measurement-Integrated Simulation International-presentation

    The seventh International Conference on Flow Dynamics 2010/11/02

  220. Fluid Analysis of the Mechanism of Fetal Brain Hemorrhage International-presentation

    The tenth International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2010) 2010/11/02

  221. Left Atrial Appendage International-presentation

    The tenth International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration (AFI/TFI-2010) 2010/11/02

  222. 医療計測との融合による血流解析

    日本機械学会第88期流体工学部門講演会 2010/10/31

  223. 血流の超音波計測融合シミュレーションにおけるゼロ表示されたドプラ速度のフィードバックの影響

    日本流体力学会年会2010 2010/09/10

  224. Computational Simulation on Convection-Enhanced Drug Delivery into the Primate Brain International-presentation

    The 6th World Congress of Biomechanics 2010/08/04

  225. Effect of Measurement Error on Ultrasonic-Measurement-Integrated Simulation of Blood Flow in an Aortic Aneurysm International-presentation

    The 13th International Symposium of 2007 Tohoku University Global COE Program “Global Nano-Biomedical Engineering Education and Research Network Centre” 2010/08/04

  226. Visualization of Microcirculation Based on Brightness Variation in Contrast-Enhanced Ultrasound International-presentation

    ASME 2010 Summer Bioengineering Conference 2010/06/17

  227. 超音波計測融合シミュレーションによる臨床応用に関する基礎的研究

    日本超音波医学会第83回学術集会 2010/05/30

  228. 超音波計測融合シミュレーションによる分岐血管の流量推定

    日本超音波医学会第83回学術集会 2010/05/29

  229. 傾斜遠心力下における赤血球挙動の流体構造連成解析

    第15回計算工学講演会 2010/05/26

  230. 大動脈瘤内における非定常血流に関する情報探査

    第15回計算工学講演会 2010/05/26

  231. 2次元下行大動脈瘤近傍における血流の計測融合シミュレーションに関する固有値解析

    日本機械学会東北支部第45期総会・講演会 2010/03/12

  232. Clinical Application of Ultrasonic-Measurement-Integrated Simulation for Diagnosis of Carotid Artery International-presentation

    SMART-Tohoku GCOE joint Workshop on Micro & Nano Bioengineering: MIT, NUS, NTU and Tohoku 2010/01/12

  233. 集中定数モデルによる循環器系病態の動特性解析 第2報:心室中隔欠損症の考察

    第22回バイオエンジニアリング講演会 2010/01/10

  234. 超音波Bモード画像の輝度値の変動に基づく微小循環の可視化

    第22回バイオエンジニアリング講演会 2010/01/10

  235. PVA頚動脈モデルを用いた3次元超音波計測融合血流シミュレーション

    第22回バイオエンジニアリング講演会 2010/01/09

  236. Analysis of Clinical Data with Ultrasonic-Measurement-Integrated Simulation International-presentation

    The 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering 2009/12

  237. Dynamic Characteristics Analysis of Diseased Circulatory System with Lumped Parameter Model 1st Report : Heart Valve Disease International-presentation

    The 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering 2009/12

  238. Eigenvalue Analysis for Error Dynamics of Ultrasonic-Measurement-Integrated Simulation of Blood Flow in the Aneurismal Aorta International-presentation

    The 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering 2009/12

  239. Three-Dimensional Ultrasonic-Measurement-Integrated Blood flow Simulation using PVA Carotid Artery Model International-presentation

    The 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering 2009/12

  240. 血流の超音波計測融合シミュレーションに関する研究 第9報:計測ノイズの影響

    第20回バイオフロンティア講演会 2009/11/08

  241. 集中定数モデルによる循環器系病態の動特性解析 第1報:心臓弁膜症の考察

    第20回バイオフロンティア講演会 2009/11/08

  242. 超音波計測融合シミュレーションによる臨床データの解析

    第20回バイオフロンティア講演会 2009/11/07

  243. Atrial Vortex International-presentation

    The 9th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration 2009/11/05

  244. Blood Flow Analysis by Measurement-Integrated Simulation International-presentation

    The 9th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration 2009/11/05

  245. Convection-enhanced Delivery of ACNU under MRI Monitoring against Recurrent Gliomas-Development of Computational Simulation of Drug Distribution International-presentation

    The 9th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration 2009/11/05

  246. Fundamental Study of Convection-Enhanced Delivery Simulation in Rat Brain International-presentation

    The 9th International Symposium on Advanced Fluid Information and Transdisciplinary Fluid Integration 2009/11/05

  247. MRI計測に基づく数値シミュレーションを用いた左心房内壁せん断応力の検討

    第12回日本栓子検出と治療学会 2009/10/10

  248. 生体モデリングにおける音響特性の制御と超音波計測

    計測自動制御学会東北支部45周年記念学術講演会 2009/09/08

  249. Reproduction of Blood Flow Field by Numerical Simulation Integrated with Ultrasonic Measurement with Noise International-presentation

    ASME 2009 Summer Bioengineering Conference 2009/06/18

  250. 超音波計測融合シミュレーションによる血行力学情報のリアルタイム可視化に関する研究

    日本超音波医学会第82回学術集会 2009/05/24

  251. Effect of Aliasing on Ultrasonic-Measurement-Integrated Simulation of Three-Dimensional Unsteady Blood Flow International-presentation

    The 10th International Symposium of 2007 Tohoku University Global COE Program “Global Nano-Biomedical Engineering Education and Research Network Centre” 2009/04/17

  252. Fluid-Structure Coupled Ultrasonic-Measurement-Integrated Simulation of Fluid in Elastic Tube with Contraction International-presentation

    9th International Symposium of Tohoku University Global COE Programme Global Nano-Biomedical Engineering Education and Research Network Centre 2009/03

  253. 血流の超音波計測融合シミュレーションに関する研究 第8報:計測誤差の影響

    第21回バイオエンジニアリング講演会 2009/01

  254. 生体に近い音響特性を有するPVAゲルの開発

    第21回バイオエンジニアリング講演会 2009/01

  255. Numerical Evaluation of MR-Measurement-Integrated Simulation of Unsteady Hemodynamics in a Cerebral Aneurysm International-presentation

    Kenichi Funamoto, Yoshitsugu Suzuki, Toshiyuki Hayase, Takashi Kosugi, Haruo Isoda

    The 8th International Symposium of 2007 Tohoku University Global COE Program “Global Nano-Biomedical Engineering Education and Research Network Centre” 2008/12

  256. MR-Measurement-Integrated Simulation of Blood Flow in Cerebral Artery International-presentation

    Tsinghua University and IFS, Tohoku University Joint Workshop 2008/10

  257. MR計測融合シミュレーションによる脳動脈瘤内の非定常流再現の数値実験

    船本 健一, 鈴木 禎嗣, 早瀬 敏幸, 小杉 隆司, 礒田 治夫

    第19回バイオフロンティア講演会 2008/09

  258. Numerical Experiment of MR-Measurement-Integrated Simulation of Steady Blood Flow in a Cerebral Aneurysm International-presentation

    Kenichi Funamoto, Yoshitsugu Suzuki, Toshiyuki Hayase, Takashi Kosugi, Haruo Isoda

    ASME 2008 Summer Bioengineering Conference 2008/06

  259. MR計測融合シミュレーションによる脳動脈瘤内の定常流再現の数値実験

    船本 健一, 鈴木 禎嗣, 早瀬 敏幸, 小杉 隆司, 礒田 治夫

    第13回計算工学講演会 2008/05

  260. 超音波計測融合シミュレーションによる血流場の可視化

    船本 健一, 早瀬 敏幸, 劉 磊, 小笠原 正文, 地挽 隆夫, 橋本 浩, 見山 広二

    日本超音波医学会第81回学術集会 2008/05

  261. Numerical Evaluation of Ultrasonic-Measurement-Integrated Simulation of Blood Flow International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    The 5th International Bio-Fluid Symposium and Workshop 2008/03

  262. 超音波計測融合シミュレーションにおける直円管内層流の解析 (非軸対称フィードバック則による3次元解析)

    船本 健一, 劉 磊, 早瀬 敏幸

    第20回バイオエンジニアリング講演会 2008/01

  263. Effect of Time Resolution of Measurement on Ultrasonic-Measurement-Integrated Simulation of Three-Dimensional Unsteady Blood Flow in an Aneurysmal Aorta International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    The 3rd Tohoku-NUS Joint Symposium on Nano-Biomedical Engineering in the East Asian-Pacific Rim Region 2007/12

  264. Accuracy of Ultrasonic-Measurement-Integrated Simulation for Three-Dimensional Blood Flow in Aneurysmal Aorta International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    The 5th Joint ASME/JSME Fluids Engineering Conference 2007/07

  265. 血流の超音波計測融合シミュレーションに関する研究 第7報:計測の時間分解能の影響

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    第19回バイオエンジニアリング講演会 2007/01

  266. 3次元非定常血流の超音波計測融合シミュレーションに関する数値実験

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    日本機械学会第84期流体工学部門講演会 2006/10

  267. 血流の超音波計測融合シミュレーションに関する研究 第6報:3次元非定常血流解析

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    日本機械学会2006年度年次大会 2006/09

  268. Improvement of Accuracy of Blood Flow Simulation by Integrating Measurement Using Ultrasonic Doppler Method International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    The 5th World Congress of Biomechanics 2006/07

  269. Fundamental Study of Transient Characteristics of Ultrasonic-Measurement-Integrated Simulation toward Reproduction of Unsteady Blood Flows International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    ASME 2006 Summer Bioengineering Conference 2006/06

  270. 超音波ドプラ計測を融合した3次元血流解析に関する基礎的研究

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    計測自動制御学会東北支部第228回研究集会 2006/05

  271. 3次元超音波計測融合シミュレーションにおけるフィードバック点の異方的配置の影響

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    第18回バイオエンジニアリング講演会 2006/01

  272. Effect of Ultrasound Probe Placement in Ultrasonic-Measurement-Integrated Simulation of Blood Flows International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    The 12th International Conference on Biomedical Engineering (ICBME 2005) 2005/12

  273. 血流の超音波計測融合シミュレーションに関する研究 第5報:3次元非定常血流場の再現

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    第16回バイオフロンティア講演会 2005/11

  274. Effect of Feedback Domain in Ultrasonic-Measurement-Integrated Simulation on Reproduction of Blood Flow Field in an Aneurysmal Aorta International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    The 6th International Symposium on Future Medical Engineering based on Bio-nanotechnology (21st Century COE Program) 2005/11

  275. 3次元超音波計測融合血流シミュレーションのフィードバック方式に関する検討

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    日本機械学会第83期流体工学部門講演会 2005/10

  276. 3次元超音波計測融合血流シミュレーションにおけるフィードバック点配置の影響

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    日本流体力学会年会2005 2005/09

  277. 血流の超音波計測融合シミュレーションに関する研究 第4報:血行力学解析の高精度化

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    日本機械学会2005年度年次大会 2005/09

  278. Ultrasonic-Measurement-Integrated Simulation for Reproduction of Three-Dimensional Blood Flow Field in the Aorta with Aneurysm International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    ASME 2005 Summer Bioengineering Conference 2005/06

  279. 超音波計測融合血流シミュレーションの開発

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    第1回21世紀COE8大学拠点合同シンポジウム 2005/03

  280. Effect of Density of Monitoring Points for Feedback in Ultrasonic-Measurement-Integrated Simulation of Blood Flow in the Aorta with Aneurysm International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    The 3rd IASTED International Conference on Biomedical Engineering (BioMED2005) 2005/02

  281. 超音波計測融合シミュレーションの3次元フィードバック則に関する検討

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    第17回バイオエンジニアリング講演会 2005/01

  282. 血流の超音波計測融合シミュレーションにおけるエイリアジングの除去

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    計測自動制御学会東北支部40周年記念学術講演会 2004/12

  283. 血流の超音波計測融合シミュレーションに関する研究 第3報:3次元血流場の再現

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    第15回バイオフロンティア講演会 2004/11

  284. Ultrasonic-Measurement-Integrated Simulation of Blood Flow in the Aorta with an Aneurysm International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Yoshifumi Saijo, Tomoyuki Yambe

    The 4th International Symposium on Advanced Fluid Information and the 1st International Symposium on Transdisciplinary Fluid Integration (AFI/TFI-2004) 2004/11

  285. 超音波計測融合シミュレーションによる大動脈血流解析

    船本 健一, 早瀬 敏幸, 西條 芳文, 山家 智之

    日本機械学会2004年度年次大会 2004/09

  286. 超音波計測融合血流シミュレーションのフィードバック則に関する検討

    船本 健一, 早瀬 敏幸, 白井 敦, 西條 芳文, 山家 智之

    日本流体力学会年会2004 2004/08

  287. Application of Ultrasonic-Measurement-Integrated Simulation to Blood Flow International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Atsushi Shirai, Yoshifumi Saijo, Tomoyuki Yambe

    The 4th International Symposium on Future Medical Engineering based on Bio-nanotechnology (21st Century COE Program) 2004/06

  288. 血流の超音波計測融合シミュレーションに関する研究 第2報:大動脈瘤内の血流の再現

    船本 健一, 早瀬 敏幸, 白井 敦, 山家 智之, 西條 芳文

    第16回バイオエンジニアリング講演会 2004/01

  289. Reproduction of Real Blood Flow in Aorta with Ultrasonic-Measurement-Integrated Simulation

    Kenichi Funamoto, Toshiyuki Hayase, Atsushi Shirai, Tomoyuki Yambe, Yoshifumi Saijo

    東北大学流体科学研究所第15回研究発表会 2003/12

  290. Reproduction of Real Blood Flow in Aorta with Ultrasonic-Measurement-Integrated Simulation International-presentation

    Kenichi Funamoto, Toshiyuki Hayase, Atsushi Shirai, Tomoyuki Yambe, Yoshifumi Saijo

    The 3rd International Symposium on Advanced Fluid Information (AFI-2003) 2003/11

  291. 血流の超音波計測融合シミュレーションに関する研究 第1報:上流速度境界条件に起因する誤差の改善

    船本 健一, 早瀬 敏幸, 白井 敦, 山家 智之, 西條 芳文

    第14回バイオフロンティア講演会 2003/09

  292. 流れの数値解析を融合した超音波診断装置の基礎的研究

    船本 健一, 早瀬 敏幸, 白井 敦

    第45回自動制御連合講演会 2002/11

  293. 傾斜遠心力下の赤血球の挙動に関する流動数値解析

    船本 健一, 早瀬 敏幸, 白井 敦

    日本機械学会2002年度年次大会 2002/09

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Industrial Property Rights 4

  1. 超音波診断装置および血流量推定プログラム

    早瀬 敏幸, 船本 健一, 門脇 弘子

    特許第5958806号

    Property Type: Patent

  2. 超音波診断装置、血流可視化装置および制御プログラム

    早瀬 敏幸, 船本 健一, 見山 広二

    特許第5522719

    Property Type: Patent

  3. 脳性麻痺発症仔の作成方法

    木村 芳孝, 伊藤 拓哉, 船本 健一, 早瀬 敏幸

    Property Type: Patent

  4. 血流可視化診断装置

    早瀬 敏幸, 船本 健一, 白井 敦, 山家 智之, 西條 芳文

    特許第4269623

    Property Type: Patent

Research Projects 23

  1. マイクロ流体デバイスを用いた細胞群の低酸素応答の解明 Competitive

    System: The Other Research Programs

    2011/02 - Present

  2. 超音波を用いた医療イメージングに関する研究 Competitive

    System: The Other Research Programs

    2007/04 - Present

  3. Measurement-Integrated Simulation of Blood Flow Competitive

    System: The Other Research Programs

    2007/04 - Present

  4. Development of vascular permeability control method by integration of blood-brain barrier-on-a-chip and measurement-integrated simulation

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2023/04/01 - 2027/03/31

  5. 脳血管-神経ユニット・胎盤エクソソーム輸送系を軸とした胎盤-脳連関機構解明と応用

    立川 正憲, 船本 健一, 福田 達也, 吉田 あつ子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 徳島大学

    2022/04/01 - 2026/03/31

  6. 血流解剖に基づく未破裂脳動脈瘤分類および新規画像診断システムの提案

    杉山 慎一郎, 新妻 邦泰, 安西 眸, 船本 健一

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

  7. Elucidation of pathological molecular mechanisms involved in pregnancy hypertension using human placental organoid chips

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tokyo University of Agriculture and Technology

    2021/04/01 - 2024/03/31

  8. 血管壁イメージングMRIと瘤内血流のAI解析による脳動脈瘤破裂点の推定

    面高 俊介, 中川 敦寛, 新妻 邦泰, 杉山 慎一郎, 園部 真也, 遠藤 英徳, 船本 健一

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2021/04/01 - 2024/03/31

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    本研究では近年明らかになったMRIにおける脳動脈瘤壁の造影効果を破裂点の予測に応用し、さらにCFD (数値流体解析: computational fluid dynamics) 解析及びAI (人工知能: artificial intelligence) 解析を組み合わせることで、従来は主に形態学的特徴から予測していた破裂点をより高い精度で予測する手法を確立する。初年度である当該当年度は後ろ向き研究として主に患者データの収集を行った。画像データ(MRI及び脳血管撮影の3次元データ)を院内放射線サーバーからPCへ移す作業を行った。予定していた約400例分のうち2/3程終了した。移行した画像データを用いて画像解析ソフトを用いた瘤壁造影効果の評価、流体解析ソフトを用いたCFD解析を同時に進めているところである。現在のところ中間結果の段階であるが破裂点の予測にはMRIにおける瘤壁造影効果は非常に有用であるとの結果が得られつつある。すなわちくも膜下血種の患者さんで術前に造影MRIを行うことで事前に破裂点を予測しうるとの結果が得られており今後解析が進むにつれて根拠が増すものと予想される。現在移行している画像データはAI解析にも活用でき、将来的にはAI解析も進めることを視野に入れている。関連する検討として同データベースを用いて切迫破裂例における瘤壁臓絵効果に関する検討を行い論文化(Omodaka S et al. J Neurosurg. 2022)、学会発表 (脳神経外科総会2021, Stroke 2022)を行い、瘤壁造影効果の臨床的意義を示すことができており本研究成果の一部であるとともに本研究を後押しする成果が得られている。

  9. ヒトウイルス受容体を介した血液脳関門突破機構に基づくエクソソームの脳細胞標的化

    立川 正憲, 船本 健一, 福田 達也

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(B)

    Category: 基盤研究(B)

    Institution: 徳島大学

    2019/04/01 - 2023/03/31

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    高分子中枢薬の脳への薬物送達を実現するためには、脳毛細血管内皮細胞を実体とする血液脳関門(Blood-Brain Barrier, BBB)の透過技術に加え、神経細胞やグリア細胞への輸送標的化の基盤技術開発が重要である。そこで本研究は、ヒトBBBにおけるエクソソーム輸送の仕組みを解明することを目的とした。ヒト神経芽細胞腫SH-SY5Y細胞の培養上清から、超遠心分離法を用いて細胞外膜小胞の単離を行った結果、細胞外膜小胞は、平均粒子径約120 nmであり、エクソソームの物理化学的特性を有していることが示された。回収したエクソソームをPKH67で蛍光標識し、ヒトBBB in vitroモデル細胞(hCMEC/D3細胞)への取り込み活性及び温度依存性を解析した。エクソソームの細胞内取り込みは、37℃と比較し4℃において有意に低下することが示され、エクソソームのBBB輸送には、脳毛細血管内皮細胞内へのエネルギー依存的な取り込み機構が関与することが示唆された。BBB輸送の分子機構を解明するため、エクソソーム上のリガンド-BBB受容体タンパク質の相互作用を明らかにすることは重要である。そこで、網羅的定量プロテオミクスを用いて、エクソソーム上に発現するタンパクを網羅的に同定し、ヒト脳血管内皮細胞上の膜タンパク質との相互作用についてパスウエイ解析や文献情報をもとに、絞り込みを行った。以上の結果から、エクソソームをキャリアーとして用いた脳への高分子薬送達の学術的基盤構築につながるデータを蓄積することができた。

  10. Investigation of the mechanism and prevention for ischemia-reperfusion injury by using blood-brain barrier-on-a-chip

    Funamoto Kenichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

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    Microvascular network was created by culturing vascular endothelial cells and fibroblasts mixed in a fibrin gel placed in a chip with controllability of oxygen concentration and mechanical and chemical stimuli. Gas mixtures were supplied to the gas channels in the chip to generate a steady state of normoxic or hypoxic conditions at oxygen concentration of 21% or 0.3%, respectively, or repeatedly change the two oxygen conditions. Immediately after the reoxygenation from the hypoxic state, reactive oxygen species were produced, and the permeability of microvascular network was significantly increased. The same tendency of the permeability increase by changes of oxygen concentration was observed in the microvascular network that mimicked human blood-brain barrier with vascular endothelial cells, pericytes, and astrocytes.

  11. Search for prevention method of fetal dysfunction by placenta-on-a-chip

    Funamoto Kenichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2018/06/29 - 2022/03/31

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    Placenta-on-a-chip was used to reproduce a characteristic three-dimensional layered structure of human placenta, in which the syncytiotrophoblast and cytotrophoblast, as well as the fetal vasculature, are located across the stroma. Fibrin gel, fibrin gel mixed with vascular endothelial cells and fibroblast cells, and cell suspensions of placental cells were injected into the three parallel gel channels and their adjacent media channels at different timings to reveal the process of human placenta formation. In addition, quantitative evaluation of material exchange between mother and fetus was achieved by measuring permeability of the trophoblast and microvascular network under controlled culture conditions. Furthermore, we developed an experimental setup to observe the dynamics of a single layer of vascular endothelial cells under hypoxia and flow exposure, and showed that their stimuli promote morphological changes.

  12. Inflammatory responses to crystals and nanoparticles

    Nakayama Masafumi, Kinoshita Kengo

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2016/04/01 - 2019/03/31

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    The inhalation of silica dust is associated with fibrosis and lung cancer, which are triggered by macrophage inflammatory responses; however, how macrophages recognize silica remains largely unknown. Here we identify by functional expression cloning the class B scavenger receptor SR-B1 as a silica receptor. Genetic deletion of SR-B1 and masking of SR-B1 by monoclonal antibodies showed that SR-B1-mediated recognition of silica is associated with caspase-1-mediated inflammatory responses in mouse macrophages and human peripheral blood monocytes. Furthermore, SR-B1 was involved in silica-induced pulmonary inflammation in mice. These results indicate that SR-B1 is a silica receptor associated with canonical inflammasome activation.

  13. Control of Cellular Responses with 3-in-1 Chip Mimicking In Vivo Microenvironment

    Funamoto Kenichi, KAMM Roger, YOSHINO Daisuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)

    Category: Grant-in-Aid for Young Scientists (A)

    Institution: Tohoku University

    2016/04/01 - 2019/03/31

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    3-in-1 chip mimicking in vivo microenvironment was developed to simultaneously control oxygen tension and mechanical and chemical stimuli to cells. The microfluidic chip enabled to generate a uniform oxygen state down to 0.3% or a linear oxygen gradient within 15 minutes by supplying gas mixtures into the gas channels in the chip. Behavior of the human breast cancer cells suspended in type I collagen gel were then observed while controlling oxygen tension using the developed chip. As the results, it was revealed that the hypoxic environment promoted the proliferation and migration of the cancer cells. Moreover, the hypoxic response of breast cancer cells was changed by cell-cell interaction when co-cultured with vascular endothelial cells.

  14. Investigation of mechanical properties of tumor microenvironment with spatial and temporal heterogeneity of oxygen tension for control of tumor cell behavior(Fostering Joint International Research)

    Funamoto Kenichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    Institution: Tohoku University

    2017 - 2019

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    Effects of spatiotemporal heterogeneity of oxygen concentration on mechanical properties of cancer cells and the surrounding extracellular matrix were investigated. A 3-in-1 chip which reproduces in vivo microenvironment was developed and employed to simultaneously control oxygen tension and mechanical and chemical stimuli to cells. Proliferation and migration speed of human breast cancer cell (MDA-MB-231 cell line) were increased under hypoxia than those under normoxia. However, these tendencies became unclear when the cancer cells were co-cultured with the vascular endothelial cells. Contraction and degradation of collagen gel was promoted under the co-culture condition, but its speed was slowed down by hypoxic exposure. Thus, it was found that changes of the cancer cell behaviors and the surrounding extracellular matrix as well as their interactions were oxygen-dependent.

  15. New concept of local chemo- and cell-therapy for spinal cord injury using convection-enhanced drug delivery

    Sugiyama Shinichiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    Spinal cord injury has dismal prognosis even in this modern era. Convection-enhanced delivery (CED) is a method for distributing small and large molecules locally in the interstitial space of the spinal cord. Delivering these molecules to the spinal cord is otherwise difficult due to the blood spinal cord barrier. This study revealed that regional infusion of drugs into the spinal cord via CED is a promising strategy for treating spinal cord injury. In addition, we investigated the safety and efficacy of stem cell transplantation (multilineage-differentiating stress enduring cell (Muse cell) transplantation) in spinal cord injury.

  16. Challenge for development of placenta-on-a-chip

    Funamoto Kenichi, YOSHINO Daisuke, ITO Takuya, SUGIBAYASHI Rika, FUNAMOTO Kiyoe

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    A placenta-on-a-chip was developed in this study in order to reproduce the structure and function of the microenvironment of a human placenta. Media channels, which model intervillous space and fetal blood vessel, were placed flanking the three parallel gel channels which mimic the extracellular matrix. Gas channels through which gas mixtures are supplied to control the oxygen tension inside the chip were placed above the media channels. A method to form monolayers of human choriocarcinoma cells (JEG3s) and human umbilical vein endothelial cells (HUVECs) in the media channels, and a method to measure the permeability of the monolayers were established. The co-culture of JEG3s and HUVECs under a controlled oxygen tension at different concentrations showed variations of cell morphology and cell junctions, implying that oxygen tension can influence the cell-cell interaction.

  17. Multidisciplinary Approach for Arteriovenous Malformation Based on Computational Fluid Dynamics

    Shimizu Hiroaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Akita University

    2014/04/01 - 2017/03/31

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    Hemodynamics of arteriovenous malformation (AVM) was successfully analyzed using computational fluid dynamics (CFD). Four dimensional magnetic resonance imaging (4D-MRI) was also performed and compared with CFD. Hemodynamic parameters such as wall shear stress and velocity were provide by both CFD and 4D-MRI. However patients who suffered from AVM mostly had severe physical conditions, and it was difficult to create a large database during three years. <BR> On the other hand, the research team developed a new approach for diagnosing the risk factor of the recurrence after coil embolization of cerebral aneurysms. Based on it, a new software to evaluate the risk for coil embolization is under developing. In conclusion, this project became a foundation for the future development of CFD.

  18. Brain protection by local hypothermia in the acute phase of traumatic brain injury

    Tominaga Teiji, Endo Toshiki, Saito Ryuta, Nakagawa Atsuhiro, Niizuma Kuniyasu, Sugiyama Shinichiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2014/04/01 - 2016/03/31

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    The purpose of this study was to develop new methodology for brain protection. We used convection-enhanced delivery (CFD) to inject low-temperature fluid into damaged tissue in the acute phase of traumatic brain injury. First, we developed magnetic resonance method for real-time monitoring of fluid distribution during the continuous CFD injection. Second, we aimed the proof-of-concept study for the effect of local hypothermia on the damaged brain. In the animal models of traumatic brain injury, it was difficult to perfuse damaged brain tissue with cooling saline because of the rapid elevation of intracranial pressure just after the injury. Though hyperosmotic fluid was reported to be effective in the CFD injection into damaged brain tissue, we could not reproduce the effectiveness of heyperosmotic fluid in our experimental design. In order to make a breakthrough, we are developing the optimal fluid to perfuse the damaged brain tissue.

  19. Prediction of the wall property and rupture risk of cerebral aneurysms based on the computational fluid dynamics

    Niizuma Kuniyasu, SUGIYAMA SHIN-ICHIRO, NAKAGAWA ATSUHIRO, HAYASE TOSHIYUKI, OHTA MAKOTO, FUNAMOTO KENICHI, ARAFUNE TATSUHIKO, WASHIO TOSHIKATSU

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)

    Category: Grant-in-Aid for Young Scientists (A)

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

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    The purpose of this study was to build a large-scale cerebral aneurysm database including computational fluid dynamics (CFD) analysis, and to predict wall property and rupture risk. In the 3 years around 500 cases were included. Based on the database, we reported that the yellowish wall can be predicted by CFD. In addition, we revealed that rupture risk or recanalization after coil embolization could be predicted by CFD. Further analysis based on the database and outpatient clinic system are planned.

  20. Convection-enhanced drug delivery into the spinal cord

    SHIN-ICHIRO Sugiyama, ENDO Toshiki, FUNAMOTO Kenichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    Convection-enhanced delivery (CED) is a method for distributing small and large molecules locally in the interstitial space of the spinal cord. Delivering these molecules to the spinal cord is otherwise difficult due to the blood-brain or spinal cord barrier. This study revealed regional distribution of macromolecules in the gray and white matter of the spinal cord. In addition, we developed computational methods to predict drug distribution after CED. Finally, we conducted CED treatment for patients with malignant spinal tumor under the approval of the ethical board in Tohoku University Hospital, and got excellent results.

  21. Blood flow simulation integrated with ultrasonic measurement of small animals

    FUNAMOTO Kenichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2009 - 2010

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    Feasibility study of ultrasonic-measurement-integrated (UMI) simulation, in which feedback signals are added to the governing equations based on errors between ultrasonic measurement and numerical simulation, was performed to obtain accurate and detailed information of hemodynamics in small animals. Flow channel whose ultrasound image was similar to that of an artery in a small animal was made from PVA gel. By experiments measuring flows in flow channels and real blood flows in mouse carotid artery or in human finger artery, the computational accuracy of UMI simulation was improved, showing the feasibility of in vivo measurement.

  22. Development of blood flow simulation integrated with ultrasonic measurement for small animals

    FUNAMOTO Kenichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (Start-up)

    Category: Grant-in-Aid for Young Scientists (Start-up)

    Institution: Tohoku University

    2007 - 2008

  23. 数値解析と超音波計測を融合した血流構造の解析

    船本 健一

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 東北大学

    2004 - 2006

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    本研究は、血流の超音波計測と数値シミュレーションを一体化した超音波計測融合シミュレーション技術を確立し、血管内の血流場および血行力学の正確かつ詳細な情報を医療現場に提供することを目的としている。本手法では、計算領域内に定義したフィードバック領域内の複数のフィードバック点において、超音波により計測可能な速度情報(ドプラ速度)を基に、実際の血流場に対する速度場の計算結果の誤差を推定し、それに基づく信号をフィードバックして計算を行う。このフィードバックの適用により、計算結果を実際の流れ場に収束させる。 本年度は、これまでの知見に基づき、実際の血流場の状態である3次元非定常流の再現に対する本手法の有用性を検証するため、動脈瘤を有する血管内の実際の血流を模擬した基準解の再現に関する数値実験を行った。実際の超音波計測には時間分解能に制限があり、計算時間刻みに対して粗い時間間隔の計測しか行えない可能性がある。そこで、計測の時間分解能が不十分な場合におけるフィードバック方法について検討した。計測データが得られた時刻のみにフィードバックを行う間欠的な方法により、各タイムステップにおいてフィードバックを適用する方法と同じ計算精度が実現でき、有用であることが明らかとなった。ただし、計測の時間間隔を大きくするのに伴い計算が不安定になり易く、ゲインが比較的小さい場合においても解が振動したり計算が発散したりする現象が見られた。 動脈瘤内の速度場の誤差が最小となる条件において本手法の計算精度を評価すると、プローブを動脈瘤と同じ高さに1つ設定した場合に、速度場および圧力場の誤差は29%、58%にまでそれぞれ減少し、プローブを2つ用いると計算精度は一層向上し、誤差は8%、17%にまで減少した。壁せん断応力分布もフィードバックの適用により基準解のものに近づき、例えば平均壁せん断応力の最大値は誤差1%以下の精度で再現できた。

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Teaching Experience 7

  1. Fluid mechanics II Ishinomaki Senshu University

  2. Fluid mechanics I Ishinomaki Senshu University

  3. Intelligent Mechanosystem Analysis Tohoku University

  4. Fluid mechanics II Tohoku University

  5. 創薬分析・理論化学持論 徳島大学

  6. 基礎ゼミ 東北大学

  7. 医工学概論 東北大学

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Social Activities 4

  1. 青森県立弘前高等学校 職業人講話

    2020/10/09 -

  2. 青森県立弘前高等学校 職業人講話

    2017/10/14 -

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    青森県立弘前高等学校1年生対象に、「医工学のおもしろさ」と題して、医工学研究者・大学教員として働くことの意義やその職業に求められる資質・能力等について講演を行った。

  3. サイエンスデイ2013

    2013/07/21 -

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    研究内容を一般の方、特に小学生を対象にわかり易く説明し、体験してもらう展示を行った。「私たちの体の中では、体じゅうに栄養や酸素を運び、老廃物(体の中のいらないもの)を集めるため、血液が血管の中をかけめぐっています。血液や血管の病気を探したり治したりするために、病院では色々な装置が活躍しています。私たちは、病気をもっと簡単に早く見つけ、そして体に優しく治すための研究をしています。ここでは、体の中の血管の形や、血管の中に入って病気を治すのに使うカテーテルや体の中を映し出す超音波装置を実際に触れてみよう。」

  4. サイエンスデイ2012

    2012/07/15 -

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    研究内容を一般の方、特に小学生を対象にわかり易く説明し、体験してもらう展示を行った。「病院では色々な装置が病気の診断や治療に活躍しています。私たちは、もっと簡単にそして優しく治せる装置の研究をしています。例えば、カテーテルという細くて柔らかいチューブは、血管の中に入っていって、手が届かないからだの奥底にある病気を治療します。血管のモデルの中にカテーテルを通して、どれだけ速くゴールまで到達できるか挑戦してみよう。さらに、血管のどこに病気があるかわかるかな?迷路血管の中を通って病気までたどり着こう。また、超音波を体にあてることで、目では直接見ることができない体の中を映すことができます。超音波装置を操って、黒い物体の中に隠れているものを探しあててみよう。」

Academic Activities 3

  1. Journal of Fluid Science and Technology

    2019/09 - Present

    Activity type: Peer review

  2. Journal of Biomechanical Science and Engineering

    2018/04/01 - 2022/03/31

    Activity type: Peer review

  3. Frontiers in Cell and Developmental Biology

    2021/10 - 2022/03

    Activity type: Peer review

Other 14

  1. 酸素濃度制御マイクロ流体デバイスによるがん微小環境の低酸素応答の解明

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    がん組織の内部は、細胞の過剰な増殖により低酸素状態にあるとともに、形成される血管が未熟であるため、酸素濃度の空間的な不均一性や急性の低酸素負荷と再酸素化が発生する。本研究では、3次元微小空間の酸素濃度分布を制御できるマイクロ流体デバイスを用いて生体内のがん微小環境を再現し、がん細胞と血管内皮細胞の挙動と、それらの細胞間の相互作用に対して酸素濃度の時空間的な変化が与える影響を解明する。

  2. 傾斜遠心力下の赤血球の摩擦特性に関する流体構造連成解析

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    傾斜遠心力を受けて平板上を押し付けられながら移動する赤血球の挙動について流体構造連成解析を行い、実験により得られる摩擦特性との比較を行う。

  3. 電場と流れ場の連成シミュレーションに関する研究

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    静電噴霧により生成された微粒子水の飛行挙動解析を行うことを目的とし、3次元空間内の静電界および流体の連成解析を実施した。

  4. 酸素制御マイクロ流体デバイスを用いた細胞応答の観測

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    酸素濃度に依存する細胞と基質間の相互作用を明らかにするため、マイクロ流体デバイス内に培養した細胞を非線形光学顕微鏡により経時的に観察し、細胞の遊走を評価する研究を行っている。

  5. 3次元培養下の細胞周囲の酸素濃度を制御するマイクロ流体デバイスの開発

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    マイクロ流体デバイスを用いることにより、これまで難しかった培養下の細胞の3次元リアルタイム観察が可能となる。本研究では、細胞周囲の酸素濃度を時間的および空間的に制御しながら細胞実験を行うためのマイクロ流体デバイスを開発している。

  6. 生体軟組織内の石灰化検出に関する研究

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    乳がんの早期発見を目的に、超音波計測により生体軟組織内の微細石灰化が造影されるメカニズムについてモデル実験を行うと共に、より効果的に石灰化を検出するため超音波の送波方法について検討を行っている。

  7. 脳動脈瘤内血流のMR計測融合シミュレーション

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    脳動脈瘤の壁面性状に関して、血流解析結果と手術中に得られる臨床所見との比較や、脳動脈瘤内の血流場や血行力学を正確に再現するためのMR計測融合シミュレーションのフィージビリティスタディを行っている。

  8. 左心房内血流の数値シミュレーション

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    心房の能動的な収縮が欠如し、心房壁が小刻みに振動する心房細動の発症による左心房内の血流場や血行力学の変化について数値シミュレーションによる検討を行っている。

  9. 脳腫瘍に対するConvection-Enhanced Delivery (CED)治療の数値シミュレーション

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    脳内の腫瘍に対して薬剤を直接投与するCED治療について、効果的に薬剤を送達するための方法について、数値シミュレーションによる検討を行っている。

  10. 胎児脳出血の機序の解明と予防法の確立

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    小児脳性麻痺の予防を目的に、その主な原因である胎児脳出血に関する動物実験を行っている。小動物の胎仔に対して虚血再還流処置を行い、超音波診断装置や胎児心電図を用いて脳出血の好発部位やタイミングを明らかにし、その予防方法について検討を行っている。

  11. 血流のMR計測融合シミュレーション手法の開発

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    脳の血管内の血流情報を高分解能かつ高精度に得るため、血流のPhase-Contrast MRIと数値シミュレーションを融合したMR計測融合シミュレーション手法を開発し、脳動脈瘤内の血流場や血行力学を再現する手法を確立した。

  12. 2次元超音波計測融合血流解析システムの開発

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    臨床応用を目的とした2次元超音波計測融合シミュレーションによる血流可視化システムについて、複数の患者の頚動脈内の血流を解析対象としたフィージビリティスタディを行い、システムの高精度化および汎用化に取り組んでいる。

  13. マイクロバブルを用いた腫瘍内の微小血管構造の可視化と評価

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    がんの診断と治療を目的に、マイクロバブルを用いた超音波計測により腫瘍内部の新生血管を含む微小な血管構造の可視化を可能にし、薬剤投与による変化を評価した。

  14. 胸部動脈瘤内血流の超音波計測融合シミュレーション

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    超音波計測と数値シミュレーションを融合した血流解析(血流の超音波計測融合シミュレーション)により、胸部動脈瘤内の複雑な非定常の血流場および血行力学を正確かつ詳細に再現する手法を開発している。

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