Details of the Researcher

PHOTO

Koetsu Ogasawara
Section
Institute of Development, Aging and Cancer
Job title
Professor
Degree

  • 博士（歯学）（東北大学）

e-Rad No.
30323603

Research Interests 6

  • Infection

  • Autoimmune diseases

  • Metal Allergy

  • Receptor

  • Tumor Immunity

  • NK cell

Awards 1

  1. 文部科学大臣表彰若手科学者賞

    2006

Papers 98

  1. Reconstruction of a resilient and secure community and medical care system in the coronavirus era - English translation of the Japanese opinion released from the Science Council of Japan.

    Katsuya Iijima, Masahiro Akishita, Tamao Endo, Tetsuo Ichikawa, Norio Ozaki, Kouetsu Ogasawara, Yasuki Kihara, Masafumi Kuzuya, Hiroko Komatsu, Hiroko Terasaki, Yuichiro Doki, Haruko Noguchi, Kiyoko Nishi, Yumi Nishimura, Nobuhiko Haga, Motohiko Miyachi, Seiji Yasumura, Junko Wake, Hidenori Arai

    Geriatrics & gerontology international　25　(4)　481-490　2025/04

    DOI： 10.1111/ggi.15073 　

    More details Close

    BACKGROUND: Over 3 years have passed since the outbreak of novel coronavirus disease 2019 (COVID-19), a disease associated with a high risk of severe illness and death among older individuals. This period has brought to light regional and social issues, including issues in overall and regional healthcare, that existed before the epidemic. "COVID-19-related frailty" is defined as secondary damage to health caused by inactivity and disconnection from human interaction owing to prolonged isolation among older individuals. Now in its fourth year, COVID-19 cannot be taken lightly, even though it is now a Category 5 infectious disease. Looking at it from the perspective of the Corona (COVID-19)/post-Corona (COVID-19) era and society, it is necessary to reconstruct regional communities in which active residents can resume their activities, a resilient regional society from multiple perspectives, and a medical and care system that can give the public a sense of security, all of which will lead to the development of local communities. CURRENT SITUATION AND PROBLEMS: Weak healthcare systems in emergencies such as emerging infectious diseases and disasters The COVID-19 pandemic has posed challenges in the management of older individuals in Japan. These challenges are common to those encountered with other emerging infectious diseases and disasters; however, the pandemic has emphasized the vulnerability of older adults. End-of-life care and advance care planning do not function during a contingency The COVID-19 pandemic has had a significant effect on the end-of-life (EOL) care of older adults, with the lack of implementation and dysfunction of advance care planning (ACP) identified as the biggest factors. This has made it difficult for this population to share their values, intentions, and life goals with their families and healthcare providers. Inadequate use of information and communication technology and the latest technologies Disparity in the digital field (digital divide) is more pronounced among older individuals. Consequently, the benefits of new technologies, such as digitalization and robotics, have not fully reached older individuals, leading to social isolation and frailty in this population. Various secondary health outcomes have emerged as a result of the COVID-19 pandemic The influence of misinformation and disinformation following the outbreak of the COVID-19 pandemic has accelerated secondary health outcomes, as excessive isolation in life has become prolonged. The inability of older individuals to screen information is a source of major concern. Furthermore, older adults are generally vulnerable to information technology and often face difficulty in accessing correct information. Lack of human resources in the field of public health The promotion of vaccine development, therapeutic drug development, and measures to prevent serious illnesses among older adults remain major challenges, especially following the COVID-19 pandemic. Information gathering and analysis during normal times are also important issues in the public health, medical care, and nursing care sectors. A shortage of human resources for this purpose has also become evident. CONTENT OF OPINION: The COVID-19 pandemic has led to the compilation of a vision for the future of the aging Japanese society from the viewpoint of individual health as well as from a broader viewpoint of the systems in the medical community, local community, and environment. These views will be reflected in the policies (including cross-ministerial flow) of academic associations such as the Japan Geriatrics Society; the Ministry of Health, Labor and Welfare; the Ministry of Education, Culture, Sports, Science and Technology; the Ministry of Economy, Trade and Industry; the Ministry of Land, Infrastructure, Transport and Tourism; the Cabinet Office; and various professional organizations. Healthcare systems that respond promptly to other emerging infectious diseases, disasters, and contingencies should be reconstructed As an issue that can commonly arise during the COVID-19 pandemic and other emerging infectious diseases, disasters, and other contingencies, a healthcare system designed for the older population, the most vulnerable segment of the population, must be developed. EOL care and ACP that is fully respected even in a contingency should be accelerated ACP should be implemented from an early stage, so that all parties involved can share values, intentions, and life goals with family members and healthcare personnel such that they are reflected in EOL care. This will enable older individuals to live as they desire until EOL. Use of information and communication technology and new technologies should be promoted to actively build new regional communication Disparities in the digital field (digital divide) must be eliminated to create an environment that enables everyone to benefit from digitalization. Furthermore, new regional communication systems, wherein the perspective of mobility support is key, must be created to prevent social isolation. The secondary health outcomes caused by the COVID-19 disaster among older individuals should be prevented through a multifaceted approach Utmost attention must be paid to preventing the occurrence of secondary health outcomes through a multifaceted approach that includes raising awareness regarding health maintenance and providing appropriate information related to health maintenance. Research in the field of public health must be promoted to strengthen human resource development in this area, with a focus on analyzing information on health, medical care, and long-term care from ordinary times Continuous support must be provided even before the occurrence of emergencies to facilitate basic research that will lead to clinical applications. Researchers at universities and research organizations, in particular, must strive to promote these activities. In addition, the government (local and national governments that have data and the national government that supports research and human resource development) must also commit to playing an important role in such research activities. Geriatr Gerontol Int 2025; 25: 481-490.

  2. Antibacterial properties of TiO2 layers formed by Au-sputtering and thermal oxidation of titanium under visible light Peer-reviewed

    T. Ueda, R. Koizumi, K. Ueda, K. Ito, K. Ogasawara, H. Kanetaka, T. Narushima

    J. Jpn. Inst. Metals and Mater.　88　(12)　314-323　2024/12

    Publisher: The Japan Institute of Metals and Materials

    DOI： 10.2320/jinstmet.JC202405 　

    ISSN： 0021-4876 1345-9678

    eISSN： 1880-6880 2433-7501 1347-5320

    More details Close

    To prevent infection in dental implants using photocatalytic activity under visible-light irradiation, the fabrication of Au-added TiO2 layers on Ti substrates and their antibacterial properties were studied. Pure Au and Ti-(60, 40) mol％Au alloy films with thicknesses of 10-47 nm were sputtered onto Ti, followed by thermal oxidation in air at 873 K for 1.8 ks to form TiO2 layers. The antibacterial properties against Escherichia coli, cytotoxicity, and bonding strength to Ti substrates were evaluated. The highest antibacterial activity under visible-light irradiation was obtained when the sputtered film was pure Au and its thickness was 38 nm. Compared with as-polished commercially pure Ti, the number of viable mouse osteoblast-like cells and human gingival fibroblasts on Au-added TiO2 layers increased after placement in the dark but decreased after visible-light irradiation. The best antibacterial property-bonding strength balance was achieved when the Ti-40 mol％Au sputtered film with a thickness of 42 nm was formed on Ti. To the best of our knowledge, this study is the first to report the formation of TiO2 layers with antibacterial activity under visible-light irradiation by combining Au-sputtering and thermal oxidation of Ti.   Mater. Trans. 64 (2023) 155-164に掲載

  3. Highly purified hypochlorous acid water facilitates glucose metabolism and memory formation in type 2 diabetic mice associated with altered-gut microbiota. International-journal

    Kazuki Watanabe, Yusuke Maruyama, Risako Mikami, Keiji Komatsu, Kenji Kikuchi, Kunimoto Hotta, Toshikazu Yoshikawa, Kouetsu Ogasawara, Atsuhiko Hattori, Shinichi Arakawa

    Scientific reports　14　(1)　16107-16107　2024/07/12

    DOI： 10.1038/s41598-024-67129-z 　

    More details Close

    Hypochlorous acid (HOCl) is an endogenous oxidant and chlorinating agent in mammals that is effective against a broad range of microorganisms. However, the effects of exogenous HOCl on biological processes have not been reported. In this study, the effects of highly purified slightly acidic hypochlorous acid water (HP-HAW) were investigated. After the safety of oral administration of HP-HAW was confirmed, the effects of HP-HAW on glucose homeostasis were assessed in mice. HP-HAW treatment significantly improved blood glucose levels in hyperglycemic condition. Based on the 16S rRNA sequencing, HP-HAW treatment significantly increased the diversity and changed the composition of gut microbiota by decreasing the abundance of genus Romboutsia in mice fed normal chow. In obese mice, HP-HAW administration tended to improve glucose tolerance. HP-HAW also attenuated memory impairments and changes N-methyl-d-aspartate (NMDA) receptor mRNA expression in obese mice. HP-HAW treatment suppressed Il-6 mRNA expression in the hippocampus in type 2 diabetic mice. Overall, these results support HP-HAW as a potential therapeutic agent to improve or prevent glucose tolerance and memory decline via gut microbiota alteration.

  4. Formation of the junctions between lymph follicles in the Peyer's patches even before postweaning activation. International-journal

    Anri Teshigahara, Yuri Banba, Hiromi Yoshida, Mitsuji Kaji, Zhou Zhou, Nao Koyama, Yoshifumi Sakai, Niel A Karrow, Kouetsu Ogasawara, Ryota Hirakawa, Jahidul Islam, Mutsumi Furukawa, Tomonori Nochi

    Scientific reports　14　(1)　15783-15783　2024/07/09

    DOI： 10.1038/s41598-024-65984-4 　

    More details Close

    Peyer's patches (PPs), which contain an abundance of B and T cells, play a key role in inducing pivotal immune responses in the intestinal tract. PPs are defined as aggregated lymph follicles, which consist of multiple lymph follicles (LFs) that may interact with each other in a synergistic manner. LFs are thought to be spherical in shape; however, the characteristics of their structure are not fully understood. To elucidate changes in the structure of PPs as individuals grow, we generated serial 2D sections from entire PPs harvested from mice at 2, 4, and 10 weeks of age and performed a 3D analysis using a software, Amira. Although the number of LFs in PPs was not changed throughout the experiment, the volume and surface area of LFs increased significantly, indicating that LFs in PPs develop continuously by recruiting immune cells, even after weaning. In response to the dramatic changes in the intestinal environment after weaning, the development of germinal centers (GCs) in LFs was observed at 4 and 10 weeks (but not 2 weeks) of age. In addition, GCs gradually began to form away from the center of LFs and close to the muscle layer where export lymphatic vessels develop. Importantly, each LF was joined to the adjacent LF; this feature was observed even in preweaning nonactivated PPs. These results suggest that PPs may have a unique organization and structure that enhance immune functions, allowing cells in LFs to have free access to adjacent LFs and egress smoothly from PPs to the periphery upon stimulation after weaning.

  5. Effect of age on orthodontic tooth movement in mice. International-journal

    Kayoko Kanou, Hideki Kitaura, Takahiro Noguchi, Fumitoshi Ohori, Aseel Marahleh, Ria Kinjo, Jinghan Ma, Jiayi Ren, Kouetsu Ogasawara, Itaru Mizoguchi

    Journal of dental sciences　19　(2)　828-836　2024/04

    DOI： 10.1016/j.jds.2023.09.016 　

    More details Close

    BACKGROUND/PURPOSE: The number of middle-aged and elderly orthodontic patients is increasing due to changes in age composition. It is important to investigate the detailed mechanisms of bone remodeling in orthodontic tooth movement (OTM) in the elderly. However, there are few reports on the mechanism of tooth movement in the elderly. The purpose of the present study was to analyze OTM and osteoclastogenesis in aged mice and to elucidate the mechanism. MATERIALS AND METHODS: It has been reported that tumor necrosis factor (TNF)-α plays an important role in osteoclast formation and OTM. First, 8-week-old and 78-week-old male C57BL/6J mice were subcutaneously injected with TNF-α into the calvaiae, and micro-CT, tartrate-resistant acid phosphatase (TRAP) staining, and real-time PCR were performed to evaluate osteoclast formation and bone resorption. Furthermore, osteoclastogenesis by TNF-α and receptor activator of nuclear factor-kappa B ligand (RANKL) using bone marrow cells was evaluated in vitro. Finally, a nickel-titanium closed-coil spring was attached, mesial movement of the maxillary left first molar was performed, and tooth movement distance and osteoclast formation were evaluated. RESULTS: Compared to 8-week-old mice, 78-week-old mice had decreased TNF-α-induced bone resorption, osteoclastogenesis, and TRAP and cathepsin K expression in the calvariae. In vitro osteoclast formation also decreased in 78-week-old mice. Furthermore, tooth movement distance and osteoclastogenesis were reduced. CONCLUSION: OTM decreased in aged mice, which was shown to be caused by a decrease in osteoclastogenesis. Therefore, it was suggested that it is necessary to keep in mind that tooth movement may be suppressed when treating elderly patients.

  6. Phospholipase D4 as a signature of toll-like receptor 7 or 9 signaling is expressed on blastic T-bet + B cells in systemic lupus erythematosus. International-journal

    Ken Yasaka, Tomohide Yamazaki, Hiroko Sato, Tsuyoshi Shirai, Minkwon Cho, Koji Ishida, Koyu Ito, Tetsuhiro Tanaka, Kouetsu Ogasawara, Hideo Harigae, Tomonori Ishii, Hiroshi Fujii

    Arthritis research & therapy　25　(1)　200-200　2023/10/16

    DOI： 10.1186/s13075-023-03186-5 　

    More details Close

    BACKGROUND: In systemic lupus erythematosus (SLE), autoreactive B cells are thought to develop by-passing immune checkpoints and contribute to its pathogenesis. Toll-like receptor (TLR) 7 and 9 signaling have been implicated in their development and differentiation. Although some B cell subpopulations such as T-bet + double negative 2 (DN2) cells have been identified as autoreactive in the past few years, because the upregulated surface markers of those cells are not exclusive to them, it is still challenging to specifically target autoreactive B cells in SLE patients. METHODS: Our preliminary expression analysis revealed that phospholipase D4 (PLD4) is exclusively expressed in plasmacytoid dendritic cells (pDCs) and B cells in peripheral blood mononuclear cells (PBMCs) samples. Monoclonal antibodies against human PLD4 were generated, and flow cytometry analyses were conducted for PBMCs from 23 healthy donors (HDs) and 40 patients with SLE. In vitro cell culture was also performed to study the conditions that induce PLD4 in B cells from HDs. Finally, recombinant antibodies were synthesized from subpopulations of PLD4 + B cells from a patient with SLE, and their antinuclear activity was measured through enzyme-linked immunosorbent assay. RESULTS: pDCs from both groups showed comparable frequency of surface PLD4 expression. PLD4 + B cells accounted for only a few percent of HD B cells, whereas they were significantly expanded in patients with SLE (2.1% ± 0.4% vs. 10.8% ± 1.2%, P < 0.005). A subpopulation within PLD4 + B cells whose cell size was comparable to CD38 + CD43 + plasmablasts was defined as "PLD4 + blasts," and their frequencies were significantly correlated with those of plasmablasts (P < 0.005). PLD4 + blasts phenotypically overlapped with double negative 2 (DN2) cells, and, in line with this, their frequencies were significantly correlated with several clinical markers of SLE. In vitro assay using healthy PBMCs demonstrated that TLR7 or TLR9 stimulation was sufficient to induce PLD4 on the surface of the B cells. Finally, two out of three recombinant antibodies synthesized from PLD4 + blasts showed antinuclear activity. CONCLUSION: PLD4 + B cells, especially "blastic" ones, are likely autoreactive B cells undergoing TLR stimulation. Therefore, PLD4 is a promising target marker in SLE treatment.

  7. Visible-light-induced antibacterial activity of carbon and nitrogen co-doped rutile TiO<inf>2</inf> films

    Ryusuke Koizumi, Kyosuke Ueda, Koyu Ito, Kouetsu Ogasawara, Hiroyasu Kanetaka, Takayuki Mokudai, Takayuki Narushima

    Thin Solid Films　780　139944　2023/09/01

    Publisher:

    DOI： 10.1016/j.tsf.2023.139944 　

    ISSN： 0040-6090

  8. Platelet αIIbβ3 integrin binds to SARS-CoV-2 spike protein of alpha strain but not wild type and omicron strains

    Koyu Ito, Kota Goto, Ryutaro Shirakawa, Hisanori Horiuchi, Kouetsu Ogasawara

    Biochemical and Biophysical Research Communications　657　80-85　2023/05/21

    DOI： 10.1016/j.bbrc.2023.03.057 　

    ISSN： 0006-291X

    eISSN： 1090-2104

  9. Antibacterial properties of TiO2 layers formed by Au-sputtering and thermal oxidation of titanium under visible light Peer-reviewed

    T. Ueda, R. Koizumi, K. Ueda, K. Ito, K. Ogasawara, H. Kanetaka, T. Narushima

    Mater. Trans.　63　(12)　155-164　2023/01

    DOI： 10.2320/matertrans.MT-MLA2022006 　

    ISSN： 1345-9678

  10. Palladium-Induced Temporal Internalization of MHC Class I Contributes to T Cell-Mediated Antigenicity. International-journal

    Koyu Ito, Takayuki Kanaseki, Serina Tokita, Toshihiko Torigoe, Noriyasu Hirasawa, Kouetsu Ogasawara

    Frontiers in immunology　12　736936-736936　2021

    DOI： 10.3389/fimmu.2021.736936 　

    More details Close

    Palladium (Pd) is a widely used metal and extremely important biomaterial for the reconstruction of occlusions during dental restorations. However, metallic biomaterials can cause serious allergic reactions, such as Pd-related oral mucositis seen in dentistry. Metal allergy is categorized as a type IV allergy and we demonstrated that CD8 T cells play an important role in Pd allergy previously. As TCR of CD8 T cells recognizes MHC class I/peptide complex, the antigen specificity to this complex seems to be generated during Pd allergy. However, it remains unknown if Pd affects the MHC class I/peptide complex. In this study, we investigated the behavior of the MHC class I/peptide complex in response to Pd treatment. We found that PdCl2 treatment altered peptide presentation on MHC class I and that co-culture with Pd-treated DC2.4 cells induced activation of Pd-responsive TCR-expressing T cell line. Furthermore, PdCl2 treatment induced temporal MHC class I internalization and inhibition of membrane movement suppressed Pd-induced T cell-mediated antigenicity. These data suggest that Pd-induced MHC class I internalization is critical for generation of antigenicity through a mechanism including differential peptide loading on MHC class I, which results in Pd allergy.

  11. Formation of carbon-added anatase-rich TiO2 layers on titanium and their antibacterial properties in visible light

    Takatoshi Ueda, Naoki Sato, Ryusuke Koizumi, Kyosuke Ueda, Koyu Ito, Kouetsu Ogasawara, Takayuki Narushima

    Dental Materials　37　(2)　e37-e46　2020/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.dental.2020.10.009 　

    ISSN： 0109-5641

  12. Point mutation bias in SARS-CoV-2 variants results in increased ability to stimulate inflammatory responses. International-journal

    Masato Kosuge, Emi Furusawa-Nishii, Koyu Ito, Yoshiro Saito, Kouetsu Ogasawara

    Scientific reports　10　(1)　17766-17766　2020/10/20

    DOI： 10.1038/s41598-020-74843-x 　

    More details Close

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection induces severe pneumonia and is the cause of a worldwide pandemic. Coronaviruses, including SARS-CoV-2, have RNA proofreading enzymes in their genomes, resulting in fewer gene mutations than other RNA viruses. Nevertheless, variants of SARS-CoV-2 exist and may induce different symptoms; however, the factors and the impacts of these mutations are not well understood. We found that there is a bias to the mutations occurring in SARS-CoV-2 variants, with disproportionate mutation to uracil (U). These point mutations to U are mainly derived from cytosine (C), which is consistent with the substrate specificity of host RNA editing enzymes, APOBECs. We also found the point mutations which are consistent with other RNA editing enzymes, ADARs. For the C-to-U mutations, the context of the upstream uracil and downstream guanine from mutated position was found to be most prevalent. Further, the degree of increase of U in SARS-CoV-2 variants correlates with enhanced production of cytokines, such as TNF-α and IL-6, in cell lines when compared with stimulation by the ssRNA sequence of the isolated virus in Wuhan. Therefore, RNA editing is a factor for mutation bias in SARS-CoV-2 variants, which affects host inflammatory cytokines production.

  13. Organogenesis of Ileal Peyer's Patches Is Initiated Prenatally and Accelerated Postnatally With Comprehensive Proliferation of B Cells in Pigs. International-journal

    Mutsumi Furukawa, Shun Ito, Shunichi Suzuki, Daiichiro Fuchimoto, Akira Onishi, Kanae Niimi, Katsuki Usami, Guoyao Wu, Fuller W Bazer, Kouetsu Ogasawara, Kouichi Watanabe, Hisashi Aso, Tomonori Nochi

    Frontiers in immunology　11　604674-604674　2020

    DOI： 10.3389/fimmu.2020.604674 　

    More details Close

    Morphogenesis and differentiation of organs is required for subsequent functional maturation. The morphological features of Peyer's patches vary among species. In pigs, they develop extensively in the ileum as ileal Peyer's patches (IPPs). However, the role of IPPs in the porcine immune system remains to be elucidated because of a lack of complete understanding of IPP organogenesis. Results of the present study revealed that development of porcine IPPs is initiated prenatally between embryonic days 76 and 91. The process of IPP organogenesis is concomitant with increased transcriptional patterns of CXCL13 and CCL19. IPPs undergo further development postnatally by forming central, marginal, and subepithelial zones. Importantly, a large number of proliferating B cells and apoptotic cells are found in porcine IPPs postnatally, but not prenatally. The expression level of IgM in proliferating B cells depends on the zone in which distinct B cells are separately localized after birth. Specifically, IgM+ cells are predominantly found in the central zone, whereas IgM-/low cells are abundant in the marginal zone. Importantly, the cellular feature of IPPs differs from that of mesenteric lymph nodes (MLNs) where such distinct zones are not formed both prenatally and postnatally. Our findings suggest that IPPs (not MLNs) in postnatal pigs are involved in complementing functions of the primary lymphoid tissue that promotes the differentiation and maturation of B cells.

  14. 【口腔環境と全身のクロストーク】歯科金属アレルギーの予防・診断・治療に向けた疾患特異的T細胞の同定

    伊藤 甲雄, 武田 裕利, 小笠原 康悦

    炎症と免疫　27　(6)　469-473　2019/10

    Publisher: (株)先端医学社

    ISSN： 0918-8371

  15. COX-2 Induces T cell accumulation and IFN-γ production during the development of chromium allergy Peer-reviewed

    R. Sitalaksmi, K. Ito, K. Ogasawara, Y. Suto, M. Itabashi, K. Ueda, N. Hirasawa, T. Narushima, S. Higuchi, N. Sato, N. Hendrijantini, U. Kresnoadi, K. Sasaki

    Autoimmunity　52　(5-6)　228-234　2019/08

    DOI： 10.1080/08916934.2019.1662404 　

    ISSN： 0891-6934

    eISSN： 1607-842X

  16. Visible-light responsible antibacterial activity of Au-incorporated TiO2 layers formed on Ti-(0–10)at%Au alloys by air oxidation International-journal Peer-reviewed

    T. Ueda, K. Ueda, K. Ito, K. Ogasawara, T. Mokudai, H. Kanetaka, Y. Niwano, T. Narushima

    J. Biomed. Mater. Res. A　107　(5)　991-1000　2019/05

    DOI： 10.1002/jbm.a.36624 　

    More details Close

    Rutile TiO2 layers were formed on substrates of Ti-(0-10)at%Au alloys by a simple process of air oxidation, and their antibacterial activities were evaluated under visible-light irradiation (λ ≥ 400 nm). Au was introduced into the TiO2 layers on Ti-(1-10)at%Au alloys and existed as both metallic Au nanoparticles and dissolved Au3+ ions. The TiO2 layers that formed on Ti-5at%Au and Ti-10at%Au alloys exhibited visible-light photocatalytic activity, that is, degradation of stearic acid and antibacterial activity against Escherichia coli. These visible-light activities were attributed to the surface plasmon resonance of metallic Au nanoparticles and the decrease in bandgap energy caused by dissolved Au3+ ions. The formation of hydroxyl radicals observed under visible-light irradiation is attributable to antibacterial activity. From a cost perspective, a Ti-5at%Au alloy is more suitable as a substrate for the formation of a TiO2 layer with antibacterial properties than a Ti-10at%Au alloy. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 991-1000, 2019.

  17. Identification of HLA-DRB1*04:10 allele as risk allele for Japanese moyamoya disease and its association with autoimmune thyroid disease: A case-control study. International-journal Peer-reviewed

    Ryosuke Tashiro, Kuniyasu Niizuma, Seik-Soon Khor, Katsushi Tokunaga, Miki Fujimura, Hiroyuki Sakata, Hidenori Endo, Hidetoshi Inoko, Koetsu Ogasawara, Teiji Tominaga

    PloS one　14　(8)　e0220858　2019

    DOI： 10.1371/journal.pone.0220858 　

    More details Close

    BACKGROUND AND PURPOSE: Moyamoya disease (MMD) is a progressive cerebrovascular disease with unknown etiology. Growing evidence suggest its involvement of autoimmune and genetic mechanisms in the pathogenesis of MMD. This study aims to clarify the association between HLA allele and MMD. METHODS: Case-control study: the DNA of 136 MMD patients in Japan was extracted and the genotype of human leukocyte antigen (HLA) from this DNA was determined by super-high-resolution single-molecule sequence-based typing using next-generation sequencing. Next, the frequency of each HLA allele (HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DQB1, and HLA-DPB1) was compared with those in the Japanese control database. In addition, haplotype estimation was performed using the expectation maximization algorithm. RESULTS: The frequencies of the HLA-DRB1*04:10 allele (4.77% vs. 1.47% in the control group; P = 1.7 × 10-3; odds ratio [OR] = 3.35) and of the HLA-DRB1*04:10-HLA-DQB1*04:02 haplotype (haplotype frequency 4.41% vs. 1.35% in the control group; P = 2.0 × 10-3; OR = 3.37) significantly increased. The frequency of thyroid diseases, such as Graves' disease and Hashimoto thyroiditis, increased in HLA-DRB1*04:10-positive MMD patients compared with that in HLA-DRB1*04:10-negative MMD patients. CONCLUSIONS: HLA-DRB1*04:10 is a risk allele and HLA-DRB1*04:10-HLA-DQB1*04:02 a risk haplotype for MMD. In addition, HLA-DRB1*04:10 is associated with thyroid disease in MMD patients.

  18. Antibacterial activity of Ag nanoparticle-containing hydroxyapatite powders in simulated body fluids with Cl ions Peer-reviewed

    O. Gokcekaya, K. Ueda, K. Ogasawara, T. Narushima

    Mater. Chem. Phys.　223　473-478　2019/01

    DOI： 10.1016/j.matchemphys 　

  19. Nickel ions bind to HSP90β and enhance HIF-1α-mediated IL-8 expression. International-journal Peer-reviewed

    Sanki Asakawa, Ryo Onodera, Koji Kasai, Yu Kishimoto, Taiki Sato, Ryosuke Segawa, Natsumi Mizuno, Kouetsu Ogasawara, Takahiro Moriya, Masahiro Hiratsuka, Noriyasu Hirasawa

    Toxicology　395　45-53　2018/02/15

    DOI： 10.1016/j.tox.2018.01.006 　

    ISSN： 0300-483X

  20. Zinc ions have a potential to attenuate both Ni ion uptake and Ni ion-induced inflammation. International-journal Peer-reviewed

    Ryo Onodera, Sanki Asakawa, Ryosuke Segawa, Natsumi Mizuno, Kouetsu Ogasawara, Masahiro Hiratsuka, Noriyasu Hirasawa

    Scientific reports　8　(1)　2911-2911　2018/02/13

    DOI： 10.1038/s41598-018-21014-8 　

    ISSN： 2045-2322

  21. Cytotoxicity of Natural Killer Cells Activated Through NKG2D Contributes to the Development of Bronchiolitis Obliterans in a Murine Heterotopic Tracheal Transplant Model Peer-reviewed

    T. Kawakami, K. Ito, Y. Matsuda, M. Noda, A. Sakurada, Y. Hoshikawa, Y. Okada, K. Ogasawara

    American Journal of Transplantation　17　(9)　2338-2349　2017/09/01

    Publisher: Blackwell Publishing Ltd

    DOI： 10.1111/ajt.14257 　

    ISSN： 1600-6143 1600-6135

  22. In vitro evaluation of Ag-containing calcium phosphates: Effectiveness of Ag-incorporated beta-tricalcium phosphate Peer-reviewed

    Ozkan Gokcekaya, Kyosuke Ueda, Kouetsu Ogasawara, Hiroyasu Kanetaka, Takayuki Narushima

    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS　75　926-933　2017/06

    DOI： 10.1016/j.msec.2017.02.059 　

    ISSN： 0928-4931

    eISSN： 1873-0191

  23. TRAV7-2*02 Expressing CD8⁺ T Cells Are Responsible for Palladium Allergy. International-journal Peer-reviewed

    Yuri Takeda, Yoshiko Suto, Koyu Ito, Wataru Hashimoto, Tadashi Nishiya, Kyosuke Ueda, Takayuki Narushima, Tetsu Takahashi, Kouetsu Ogasawara

    International journal of molecular sciences　18　(6)　2017/05/31

    DOI： 10.3390/ijms18061162 　

    ISSN： 1422-0067

  24. IFN-gamma is required for cytotoxic T cell-dependent cancer genome immunoediting Peer-reviewed

    Kazuyoshi Takeda, Masafumi Nakayama, Yoshihiro Hayakawa, Yuko Kojima, Hiroaki Ikeda, Naoko Imai, Kouetsu Ogasawara, Ko Okumura, David M. Thomas, Mark J. Smyth

    NATURE COMMUNICATIONS　8　14607　2017/02

    DOI： 10.1038/ncomms14607 　

    ISSN： 2041-1723

  25. Ceramic coating of Ti and its alloys using dry processes for biomedical applications Peer-reviewed

    T. Ueda, N. Kondo, S. Sado, O. Gokcekaya, K. Ueda, K. Ogasawara, T. Narushima

    Interface Oral Health Science 2016, Innovative Research on Biosis-Abiosis Intelligent Interface　23-34　2017/01/08

    DOI： 10.1007/978-981-10-1560-1_2 　

  26. In vitro properties of Ag-containing calcium phosphates Peer-reviewed

    O. Gokcekaya, K. Ueda, T. Narushima, K. Ogasawara, H. Kanetaka

    Advances in Ceramic Armor, Bioceramics, and Porous Materials: Ceramic Engineering and Science Proceedings　47　(4)　87-93　2017/01

    DOI： 10.1002/9781119321682.ch10 　

  27. The potential role of Osteopontin in the maintenance of commensal bacteria homeostasis in the intestine. International-journal Peer-reviewed

    Koyu Ito, Akira Nakajima, Yuji Fukushima, Keiichiro Suzuki, Keiko Sakamoto, Yoko Hamazaki, Kouetsu Ogasawara, Nagahiro Minato, Masakazu Hattori

    PloS one　12　(3)　e0173629　2017

    DOI： 10.1371/journal.pone.0173629 　

    ISSN： 1932-6203

  28. Quantitative in vivo biocompatibility of new ultralow-nickel cobalt-chromium-molybdenum alloys Peer-reviewed

    Kazuaki Sonofuchi, Yoshihiro Hagiwara, Yuichiro Koizumi, Akihiko Chiba, Mitsuko Kawano, Masafumi Nakayama, Kouetsu Ogasawara, Yutaka Yabe, Eiji Itoi

    JOURNAL OF ORTHOPAEDIC RESEARCH　34　(9)　1505-1513　2016/09

    DOI： 10.1002/jor.23150 　

    ISSN： 0736-0266

    eISSN： 1554-527X

  29. The antihistamine olopatadine regulates T cell activation in palladium allergy Peer-reviewed

    Naohiko Iguchi, Yuri Takeda, Naoki Sato, Kenichirou Ukichi, Akira Katakura, Kyosuke Ueda, Takayuki Narushima, Shigehito Higuchi, Kouetsu Ogasawara

    INTERNATIONAL IMMUNOPHARMACOLOGY　35　70-76　2016/06

    DOI： 10.1016/j.intimp.2016.03.021 　

    ISSN： 1567-5769

    eISSN： 1878-1705

  30. Activation of the NLRP3 inflammasome by cellular labile iron Peer-reviewed

    Kyohei Nakamura, Toru Kawakami, Naoki Yamamoto, Miyu Tomizawa, Tohru Fujiwara, Tomonori Ishii, Hideo Harigae, Kouetsu Ogasawara

    EXPERIMENTAL HEMATOLOGY　44　(2)　116-124　2016/02

    DOI： 10.1016/j.exphem.2015.11.002 　

    ISSN： 0301-472X

    eISSN： 1873-2399

  31. In vitro evaluation of Ag-containing calcium phosphates Peer-reviewed

    O. Gokcekaya, K. Ueda, T. Narushima, K. Ogasawara

    Proceedings of the 24th International Symposium on Processing and Fabrication of Advanced Materials, PFAM XXIV　95-103　2015/12/18

  32. Control of IFN-γ production and regulatory function by the inducible nuclear protein IκB-ζ in T cells

    Takashi MaruYama, Shuhei Kobayashi, Kouetsu Ogasawara, Akihiko Yoshimura, WanJun Chen, Tatsushi Muta

    Journal of Leukocyte Biology　98　(3)　385-393　2015/09

    Publisher: Wiley

    DOI： 10.1189/jlb.2a0814-384r 　

    ISSN： 0741-5400

  33. Control of IFN-gamma production and regulatory function by the inducible nuclear protein I kappa B-zeta in T cells Peer-reviewed

    Takashi MaruYama, Shuhei Kobayashi, Kouetsu Ogasawara, Akihiko Yoshimura, WanJun Chen, Tatsushi Muta

    JOURNAL OF LEUKOCYTE BIOLOGY　98　(3)　385-393　2015/09

    DOI： 10.1189/jlb.2A0814-384R 　

    ISSN： 0741-5400

    eISSN： 1938-3673

  34. Evaluation of photocatalytic activity of the TiO2 layer formed on Ti by thermal oxidation Peer-reviewed

    T. Narushima, S. Sado, N. Kondo, K. Ueda, M. Kawano, K. Ogasawara

    Interface Oral Health Science 2014, Innovative Research on Biosis-Abiosis Intelligent Interface　65-78　2015/02/01

    DOI： 10.1007/978-4-431-55192-8_6 　

  35. Macrophage with gold nanorod visualized by optical-resolution and acoustic-resolution photoacoustic microscopes. International-journal

    Rena Yamazaki, Koetsu Ogasawara, Mitsuhiro Fujiwara, Kazuto Kobayashi, Yoshifumi Saijo

    37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society(EMBC)　2015　2387-2390　2015

    Publisher: IEEE

    DOI： 10.1109/EMBC.2015.7318874 　

    More details Close

    Macrophages play a key role in inflammation and they are frequently observed in vulnerable atherosclerotic plaque. In the present study, macrophages phagocytosing gold nanorod (AuNR) were observed by optical-resolution (OR) and acoustic-resolution (AR) photoacoustic microscope (PAM). The OR-PAM consisted of diode laser optically focused to 60 micron and planar ultrasonic transducer with the central frequency of 8 MHz placed under the object. AR-PAM consisted of concave ultrasonic transducer with the central frequency of 20 MHz and optical fiber through the center hole of the transducer for laser irradiation. First, PA signal from gold, silver and copper wire were measured in order to determine the best metal substrate for enhancing PA contrast. Gold generated largest PA signal. AuNR with the resonance wavelength of 1064 nm was co-cultured with the macrophages for phagocytosis. PA signal was successfully detected from macrophages with AuNR by both OR-PAM and AR-PAM. PA imaging of the macrophages with AuNR indicates inflammation in the vulnerable plaque and AR-PAM method would be applicable for clinical settings.

  36. Pathological analysis of metal allergy to metallic materials Peer-reviewed

    Mitsuko Kawano, Yuri Takeda, Kouetsu Ogasawara

    Springer Series in Biomaterials Science and Engineering　3　305-321　2015

    Publisher: Springer New York LLC

    DOI： 10.1007/978-3-662-46836-4_13 　

    ISSN： 2195-0652 2195-0644

  37. Comparison of CD163(+) Macrophages and CD206(+) Cells in Lesional Skin of CD30(+) Lymphoproliferative Disorders of Lymphomatoid Papulosis and Primary Cutaneous Anaplastic Large-cell Lymphoma Peer-reviewed

    Aya Kakizaki, Taku Fujimura, Yumi Kambayashi, Sadanori Furudate, Mitsuko Kawano, Kouetsu Ogasawara, Setsuya Aiba

    ACTA DERMATO-VENEREOLOGICA　95　(5)　600-602　2015

    DOI： 10.2340/00015555-2016 　

    ISSN： 0001-5555

    eISSN： 1651-2057

  38. NKG2D functions as an activating receptor on natural killer cells in the common marmoset (Callithrix jacchus) Peer-reviewed

    Masamichi Watanabe, Yohei Kudo, Mitsuko Kawano, Masafumi Nakayama, Kyohei Nakamura, Mai Kameda, Masamune Ebara, Takeki Sato, Marina Nakamura, Kaito Omine, Yoshie Kametani, Ryuji Suzuki, Kouetsu Ogasawara

    INTERNATIONAL IMMUNOLOGY　26　(11)　597-606　2014/11

    DOI： 10.1093/intimm/dxu053 　

    ISSN： 0953-8178

    eISSN： 1460-2377

  39. Effect of Silica Particle Size on Macrophage Inflammatory Responses Peer-reviewed

    Toshimasa Kusaka, Masafumi Nakayama, Kyohei Nakamura, Mai Ishimiya, Emi Furusawa, Kouetsu Ogasawara

    PLOS ONE　9　(3)　e92634　2014/03

    DOI： 10.1371/journal.pone.0092634 　

    ISSN： 1932-6203

  40. NKG2D(+) IFN-gamma(+) CD8(+) T Cells Are Responsible for Palladium Allergy Peer-reviewed

    Mitsuko Kawano, Masafumi Nakayama, Yusuke Aoshima, Kyohei Nakamura, Mizuho Ono, Tadashi Nishiya, Syou Nakamura, Yuri Takeda, Akira Dobashi, Akiko Takahashi, Misato Endo, Akiyo Ito, Kyosuke Ueda, Naoki Sato, Shigehito Higuchi, Takeru Kondo, Suguru Hashimoto, Masamichi Watanabe, Makoto Watanabe, Tetsu Takahashi, Keiichi Sasaki, Masanori Nakamura, Takehiko Sasazuki, Takayuki Narushima, Ryuji Suzuki, Kouetsu Ogasawara

    PLOS ONE　9　(2)　e86810　2014/02

    DOI： 10.1371/journal.pone.0086810 　

    ISSN： 1932-6203

  41. 細菌感染におけるペア型受容体の役割

    中山 勝文, 小笠原 康悦

    Surgery Frontier　21　41-45　2014

  42. Approach for Development of Novel Biomaterials Based on Immunological Analysis

    Kawano Mitsuko, Takeda Yuri, Nakamura Sho, Ogasawara Kouetsu

    Bulletin of the Japan Institute of Metals　53　(4)　153-156　2014

    Publisher: The Japan Institute of Metals and Materials

    DOI： 10.2320/materia.53.153 　

    ISSN： 1340-2625

    eISSN： 1884-5843

  43. Accumulation of Metal-Specific T Cells in Inflamed Skin in a Novel Murine Model of Chromium-Induced Allergic Contact Dermatitis Peer-reviewed

    Hiroaki Shigematsu, Kenichi Kumagai, Hiroshi Kobayashi, Takanori Eguchi, Kazutaka Kitaura, Satsuki Suzuki, Tatsuya Horikawa, Takaji Matsutani, Kouetsu Ogasawara, Yoshiki Hamada, Ryuji Suzuki

    PLOS ONE　9　(1)　e85983　2014/01

    DOI： 10.1371/journal.pone.0085983 　

    ISSN： 1932-6203

  44. Successful Treatment of Adult Onset Langerhans Cell Histiocytosis with Bi-weekly Administration of Pegylated Interferon-alpha Peer-reviewed

    Sadanori Furudate, Taku Fujimura, Yumi Kambayashi, Mitsuko Kawano, Kouetsu Ogasawara, Akira Tsukada, Erika Tamabuchi, Takahiro Haga, Akira Hashimoto, Setsuya Aiba

    ACTA DERMATO-VENEREOLOGICA　94　(5)　611-612　2014

    DOI： 10.2340/00015555-1807 　

    ISSN： 0001-5555

    eISSN： 1651-2057

  45. High frequencies of positive nickel/cobalt patch tests and high sweat nickel concentration in patients with intrinsic atopic dermatitis Peer-reviewed

    Hayato Yamaguchi, Rieko Kabashima-Kubo, Toshinori Bito, Jun-ichi Sakabe, Takatoshi Shimauchi, Taisuke Ito, Satoshi Hirakawa, Noriyasu Hirasawa, Koetsu Ogasawara, Yoshiki Tokura

    JOURNAL OF DERMATOLOGICAL SCIENCE　72　(3)　240-245　2013/12

    DOI： 10.1016/j.jdermsci.2013.07.009 　

    ISSN： 0923-1811

    eISSN： 1873-569X

  46. Photocatalytic evaluation of anatase layer formed on Ti alloys by thermal oxidation Peer-reviewed

    S. Sado, K. Ueda, T. Narushima, M. Kawano, K. Ogasawara

    Proceedings for International Symposium on Eco Topia Science 2013　1131　2013/11

  47. NK-cell fratricide Dynamic crosstalk between NK and cancer cells Peer-reviewed

    Kyohei Nakamura, Masafumi Nakayama, Mitsuko Kawano, Tomonori Ishii, Hideo Harigae, Kouetsu Ogasawara

    ONCOIMMUNOLOGY　2　(11)　e26529　2013/11

    DOI： 10.4161/onci.26529 　

    ISSN： 2162-4011

    eISSN： 2162-402X

  48. Characterization of T Cell Receptors of Th1 Cells Infiltrating Inflamed Skin of a Novel Murine Model of Palladium-Induced Metal Allergy Peer-reviewed

    Hiroshi Kobayashi, Kenichi Kumagai, Takanori Eguchi, Hiroaki Shigematsu, Kazutaka Kitaura, Mitsuko Kawano, Tatsuya Horikawa, Satsuki Suzuki, Takaji Matsutani, Kouetsu Ogasawara, Yoshiki Hamada, Ryuji Suzuki

    PLOS ONE　8　(10)　e76385　2013/10

    DOI： 10.1371/journal.pone.0076385 　

    ISSN： 1932-6203

  49. Accumulation of invariant NKT cells into inflamed skin in a novel murine model of nickel allergy Peer-reviewed

    Takanori Eguchi, Kenichi Kumagai, Hiroshi Kobayashi, Hiroaki Shigematsu, Kazutaka Kitaura, Satsuki Suzuki, Tatsuya Horikawa, Yoshiki Hamada, Kouetsu Ogasawara, Ryuji Suzuki

    CELLULAR IMMUNOLOGY　284　(1-2)　163-171　2013/07

    DOI： 10.1016/j.cellimm.2013.07.010 　

    ISSN： 0008-8749

    eISSN： 1090-2163

  50. Fratricide of natural killer cells dressed with tumor-derived NKG2D ligand Peer-reviewed

    Kyohei Nakamura, Masafumi Nakayama, Mitsuko Kawano, Ryo Amagai, Tomonori Ishii, Hideo Harigae, Kouetsu Ogasawara

    Proceedings of the National Academy of Sciences of the United States of America　110　(23)　9421-9426　2013/06/04

    DOI： 10.1073/pnas.1300140110 　

    ISSN： 0027-8424 1091-6490

  51. Comparison of Interleukin-17-Producing Cells in Different Clinical Types of Alopecia Areata Peer-reviewed

    Genichi Tojo, Taku Fujimura, Mitsuko Kawano, Kouetsu Ogasawara, Yumi Kambayashi, Sadanori Furudate, Masato Mizuashi, Setsuya Aiba

    DERMATOLOGY　227　(1)　78-82　2013

    DOI： 10.1159/000353159 　

    ISSN： 1018-8665

    eISSN： 1421-9832

  52. Inhibitory Receptor Paired Ig-like Receptor B Is Exploited by Staphylococcus aureus for Virulence Peer-reviewed

    Masafumi Nakayama, Kenji Kurokawa, Kyohei Nakamura, Bok Luel Lee, Kazuhisa Sekimizu, Hiromi Kubagawa, Keiichi Hiramatsu, Hideo Yagita, Ko Okumura, Toshiyuki Takai, David M. Underhill, Alan Aderem, Kouetsu Ogasawara

    JOURNAL OF IMMUNOLOGY　189　(12)　5903-5911　2012/12

    DOI： 10.4049/jimmunol.1201940 　

    ISSN： 0022-1767

  53. A new method for quantitative analysis of the T cell receptor V region repertoires in healthy common marmosets by microplate hybridization assay Peer-reviewed

    Kazutaka Kitaura, Yoshiki Fujii, Takaji Matsutani, Kenji Shirai, Satsuki Suzuki, Tomohiko Takasaki, Shin Shimada, Yoshie Kametani, Takashi Shiina, Shuji Takabayashi, Hideki Katoh, Kouetsu Ogasawara, Ichiro Kurane, Ryuji Suzuki

    JOURNAL OF IMMUNOLOGICAL METHODS　384　(1-2)　81-91　2012/10

    DOI： 10.1016/j.jim.2012.07.012 　

    ISSN： 0022-1759

    eISSN： 1872-7905

  54. A group of atopic dermatitis without IgE elevation or barrier impairment shows a high Th1 frequency: Possible immunological state of the intrinsic type Peer-reviewed

    Rieko Kabashima-Kubo, Motonobu Nakamura, Jun-ichi Sakabe, Kazunari Sugita, Ryosuke Hino, Tomoko Mori, Miwa Kobayashi, Toshinori Bito, Kenji Kabashima, Koetsu Ogasawara, Yukiko Nomura, Toshifumi Nomura, Masashi Akiyama, Hiroshi Shimizu, Yoshiki Tokura

    JOURNAL OF DERMATOLOGICAL SCIENCE　67　(1)　37-43　2012/07

    DOI： 10.1016/j.jdermsci.2012.04.004 　

    ISSN： 0923-1811

    eISSN： 1873-569X

  55. Blocking of CTLA-4 on lymphocytes improves the sensitivity of lymphocyte transformation tests in a patient with nickel allergy Peer-reviewed

    Kazunari Sugita, Kenji Kabashima, Yu Sawada, Sanehito Haruyama, Manabu Yoshioka, Tomoko Mori, Miwa Kobayashi, Kouetsu Ogasawara, Yoshiki Tokura

    EUROPEAN JOURNAL OF DERMATOLOGY　22　(2)　268-269　2012/03

    DOI： 10.1684/ejd.2012.1641 　

    ISSN： 1167-1122

    eISSN： 1952-4013

  56. Natural killer (NK)-dendritic cell interactions generate MHC class II-dressed NK cells that regulate CD4(+) T cells Peer-reviewed

    Masafumi Nakayama, Kazuyoshi Takeda, Mitsuko Kawano, Toshiyuki Takai, Naoto Ishii, Kouetsu Ogasawara

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　108　(45)　18360-18365　2011/11

    DOI： 10.1073/pnas.1110584108 　

    ISSN： 0027-8424

  57. IFN-gamma production by lung NK cells is critical for the natural resistance to pulmonary metastasis of B16 melanoma in mice Peer-reviewed

    Kazuyoshi Takeda, Masafumi Nakayama, Masashi Sakaki, Yoshihiro Hayakawa, Michio Imawari, Kouetsu Ogasawara, Ko Okumura, Mark J. Smyth

    JOURNAL OF LEUKOCYTE BIOLOGY　90　(4)　777-785　2011/10

    DOI： 10.1189/jlb.0411208 　

    ISSN： 0741-5400

  58. Increased Positive Selection Pressure Within the Complementarity Determining Regions of the T-Cell Receptor beta Gene in New World Monkeys Peer-reviewed

    Takaji Matsutani, Yoshiki Fujii, Kazutaka Kitaura, Satsuki Suzuki, Yuji Tsuruta, Tomohiko Takasaki, Kouetsu Ogasawara, Norihiro Nishimoto, Ichiro Kurane, Ryuji Suzuki

    AMERICAN JOURNAL OF PRIMATOLOGY　73　(10)　1082-1092　2011/10

    DOI： 10.1002/ajp.20976 　

    ISSN： 0275-2565

  59. The ECS(SPSB) E3 ubiquitin ligase is the master regulator of the lifetime of inducible nitric-oxide synthase Peer-reviewed

    Kazuma Matsumoto, Tadashi Nishiya, Satoshi Maekawa, Takahiro Horinouchi, Kouetsu Ogasawara, Takashi Uehara, Soichi Miwa

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　409　(1)　46-51　2011/05

    DOI： 10.1016/j.bbrc.2011.04.103 　

    ISSN： 0006-291X

  60. Regulation of Inducible Nitric-oxide Synthase by the SPRY Domain- and SOCS Box-containing Proteins Peer-reviewed

    Tadashi Nishiya, Kazuma Matsumoto, Satoshi Maekawa, Emi Kajita, Takahiro Horinouchi, Masahiro Fujimuro, Kouetsu Ogasawara, Takashi Uehara, Soichi Miwa

    JOURNAL OF BIOLOGICAL CHEMISTRY　286　(11)　9009-9019　2011/03

    DOI： 10.1074/jbc.M110.190678 　

    ISSN： 0021-9258

  61. Intact NKG2D-Independent Function of NK Cells Chronically Stimulated with the NKG2D Ligand Rae-1 Peer-reviewed

    Marine Champsaur, Joshua N. Beilke, Kouetsu Ogasawara, Ulrich H. Koszinowski, Stipan Jonjic, Lewis L. Lanier

    JOURNAL OF IMMUNOLOGY　185　(1)　157-165　2010/07

    DOI： 10.4049/jimmunol.1000397 　

    ISSN： 0022-1767

  62. Compensatory role of Langerhans cells and langerin-positive dermal dendritic cells in the sensitization phase of murine contact hypersensitivity Peer-reviewed

    Tetsuya Honda, Saeko Nakajima, Gyohei Egawa, Kouetsu Ogasawara, Bernard Malissen, Yoshiki Miyachi, Kenji Kabashima

    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY　125　(5)　1154-1156　2010/05

    DOI： 10.1016/j.jaci.2009.12.005 　

    ISSN： 0091-6749

  63. Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus Peer-reviewed

    Silvia Vilarinho, Kouetsu Ogasawara, Stephen Nishimura, Lewis L. Lanier, Jody L. Baron

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　104　(46)　18187-18192　2007/11

    DOI： 10.1073/pnas.0708968104 　

    ISSN： 0027-8424

  64. Genomic analysis of RAE-1 genes Peer-reviewed

    Kouetsu Ogasawara, Kazusa Ishizaki, Naruyoshi Fujiwara, Takehiko Sasazuki

    JOURNAL OF IMMUNOLOGY　178　2007/04

    ISSN： 0022-1767

    eISSN： 1550-6606

  65. NKG2D in NK and T cell-mediated immunity Peer-reviewed

    K Ogasawara, LL Lanier

    JOURNAL OF CLINICAL IMMUNOLOGY　25　(6)　534-540　2005/11

    DOI： 10.1007/s10875-005-8786-4 　

    ISSN： 0271-9142

  66. Function of NKG2D in natural killer cell-mediated rejection of mouse bone marrow grafts Peer-reviewed

    K Ogasawara, J Benjamin, R Takaki, JH Phillips, LL Lanier

    NATURE IMMUNOLOGY　6　(9)　938-945　2005/09

    DOI： 10.1038/ni1236 　

    ISSN： 1529-2908

  67. IFN-gamma-mediated negative feedback regulation of NKT-cell function by CD94/NKG2 Peer-reviewed

    T Ota, K Takeda, H Akiba, Y Hayakawa, K Ogasawara, Y Ikarashi, S Miyake, H Wakasugi, T Yamamura, M Kronenberg, DH Raulet, K Kinoshita, H Yagita, MJ Smyth, K Okumura

    BLOOD　106　(1)　184-192　2005/07

    DOI： 10.1182/blood-2004-11-4257 　

    ISSN： 0006-4971

  68. TRAIL identifies immature natural killer cells in newborn mice and adult mouse liver Peer-reviewed

    K Takeda, E Cretney, Y Hayakawa, T Ota, H Akiba, K Ogasawara, H Yagita, K Kinoshita, K Okumura, MJ Smyth

    BLOOD　105　(5)　2082-2089　2005/03

    DOI： 10.1182/blood-2004-08-3262 　

    ISSN： 0006-4971

  69. NK cells in innate immunity Peer-reviewed

    JA Hamerman, K Ogasawara, LL Lanier

    CURRENT OPINION IN IMMUNOLOGY　17　(1)　29-35　2005/02

    DOI： 10.1016/j.coi.2004.11.001 　

    ISSN： 0952-7915

  70. Engagement of NKG2D by cognate ligand or antibody alone is insufficient to mediate costimulation of human and mouse CD8(+) T cells Peer-reviewed

    LIR Ehrlich, K Ogasawara, JA Hamerman, R Takaki, A Zingoni, JP Allison, LL Lanier

    JOURNAL OF IMMUNOLOGY　174　(4)　1922-1931　2005/02

    ISSN： 0022-1767

  71. NKG2D blockade prevents autoimmune diabetes in NOD mice Peer-reviewed

    K Ogasawara, JA Hamerman, LR Ehrlich, H Bour-Jordan, P Santamaria, JA Bluestone, LL Lanier

    IMMUNITY　20　(6)　757-767　2004/06

    DOI： 10.1016/j.immuni.2004.05.008 　

    ISSN： 1074-7613

  72. Cutting edge: Toll-like receptor signaling in macrophages induces ligands for the NKG2D receptor Peer-reviewed

    JA Hamerman, K Ogasawara, LL Lanier

    JOURNAL OF IMMUNOLOGY　172　(4)　2001-2005　2004/02

    ISSN： 0022-1767

  73. The signaling adapter protein DAP12 regulates multinucleation during osteoclast development Peer-reviewed

    MB Humphrey, K Ogasawara, W Yao, SC Spusta, MR Daws, NE Lane, LL Lanier, MC Nakamura

    JOURNAL OF BONE AND MINERAL RESEARCH　19　(2)　224-234　2004/02

    DOI： 10.1359/JBMR.0301234 　

    ISSN： 0884-0431

  74. Activation of natural killer cells and dendritic cells upon recognition of a novel CD99-like ligand by paired immunoglobulin-like type 2 receptor. Peer-reviewed

    Shiratori I, Ogasawara K, Saito T, Lanier LL, Arase H

    The Journal of experimental medicine　199　(4)　525-533　2004/02

    DOI： 10.1084/jem.20031885 　

    ISSN： 0022-1007

  75. NKG2D triggers cytotoxicity in mouse NK cells lacking DAP12 or Syk family kinases Peer-reviewed

    S Zompi, JA Hamerman, K Ogasawara, E Schweighoffer, VLJ Tybulewicz, JP Di Santo, LL Lanier, F Colucci

    NATURE IMMUNOLOGY　4　(6)　565-572　2003/06

    DOI： 10.1038/ni930 　

    ISSN： 1529-2908

  76. NKG2D-mediated natural killer cell protection against cytomegalovirus is impaired by viral gp40 modulation of retinoic acid early inducible 1 gene molecules Peer-reviewed

    M Lodoen, K Ogasawara, JA Hamerman, H Arase, JP Houchins, ES Mocarski, LL Lanier

    JOURNAL OF EXPERIMENTAL MEDICINE　197　(10)　1245-1253　2003/05

    DOI： 10.1084/jem.20021973 　

    ISSN： 0022-1007

  77. Impairment of NK cell function by NKG2D modulation in NOD mice Peer-reviewed

    K Ogasawara, JA Hamerman, H Hsin, S Chikuma, H Bour-Jordan, T Chen, T Pertel, C Carnaud, JA Bluestone, LL Lanier

    IMMUNITY　18　(1)　41-51　2003/01

    DOI： 10.1016/S1074-7613(02)00505-8 　

    ISSN： 1074-7613

  78. Inducible costimulator costimulates cytotoxic activity and IFN-gamma production in activated murine NK cells Peer-reviewed

    K Ogasawara, SK Yoshinaga, LL Lanier

    JOURNAL OF IMMUNOLOGY　169　(7)　3676-3685　2002/10

    ISSN： 0022-1767

  79. Requirement of the IFN-alpha/beta-induced CXCR3 chemokine signalling for CD8(+) T cell activation Peer-reviewed

    K Ogasawara, S Hida, YM Weng, A Saiura, K Sato, H Takayanagi, S Sakaguchi, T Yokochi, T Kodama, M Naitoh, JA De Martino, T Taniguchi

    GENES TO CELLS　7　(3)　309-320　2002/03

    DOI： 10.1046/j.1365-2443.2002.00515.x 　

    ISSN： 1356-9597

  80. Antiviral response by natural killer cells through TRAIL gene induction by IFN-α/β

    Kojiro Sato, Shigeaki Hida, Hiroshi Takayanagi, Taeko Yokochi, Nobuhiko Kayagaki, Kazuyoshi Takeda, Hideo Yagita, Ko Okumura, Nobuyuki Tanaka, Tadatsugu Taniguchi, Kouetsu Ogasawara

    European Journal of Immunology　31　3138-3146　2001/12/11

    DOI： 10.1002/1521-4141(200111)31:11<3138::AID-IMMU3138>3.0.CO;2-B 　

  81. IRF family of transcription factors as regulators of host defense

    T Taniguchi, K Ogasawara, A Takaoka, N Tanaka

    ANNUAL REVIEW OF IMMUNOLOGY　19　623-655　2001

    DOI： 10.1146/annurev.immunol.19.1.623 　

    ISSN： 0732-0582

  82. Genes highly expressed in the early phase of murine graft-versus-host reaction Peer-reviewed

    Masatoshi Wakui, Daisuke Sakurai, Naoyuki Tsuchiya, Katsushi Tokunaga, Masatoshi Wakui, Yasuo Ikeda, Akihiro Yamaguchi, Kouetsu Ogasawara, Taeko Yokochi

    Biochemical and Biophysical Research Communications　282　(1)　200-206　2001

    DOI： 10.1006/bbrc.2001.4550 　

    ISSN： 0006-291X

  83. T-cell-mediated regulation of osteoclastogenesis by signalling cross-talk between RANKL and IFN-γ Peer-reviewed

    Hiroshi Takayanagi, Kouetsu Ogasawara, Shigeaki Hida, Tomoki Chiba, Shigeo Murata, Kojiro Sato, Akinori Takaoka, Taeko Yokochi, Hiromi Oda, Keiji Tanaka, Kozo Nakamura, Tadatsugu Taniguchi

    Nature　408　(6812)　600-605　2000/11/30

    DOI： 10.1038/35046102 　

    ISSN： 0028-0836

  84. A novel motor, KIF13A, transports mannose-6-phosphate receptor to plasma membrane through direct interaction with AP-1 complex Peer-reviewed

    Terunaga Nakagawa, Mitsutoshi Setou, Dae-Hyun Seog, Kouetsu Ogasawara, Naoshi Dohmae, Koji Takio, Nobutaka Hirokawa

    Cell　103　(4)　569-581　2000

    Publisher: Cell Press

    DOI： 10.1016/S0092-8674(00)00161-6 　

    ISSN： 0092-8674

  85. Distinct and essential roles of transcription factors IRF-3 and IRF-7 in response to viruses for IFN-α/β gene induction International-journal

    Mitsuharu Sato, Hirofumi Suemori, Naoki Hata, Masataka Asagiri, Kouetsu Ogasawara, Kazuki Nakao, Takeo Nakaya, Motoya Katsuki, Shigeru Noguchi, Nobuyuki Tanaka, Tadatsugu Taniguchi

    Immunity　13　(4)　539-548　2000/01/01

    DOI： 10.1016/S1074-7613(00)00053-4 　

    ISSN： 1074-7613

  86. Loss of transcription factor IRF-1 affects tumor susceptibility in mice carrying the Ha-ras transgene or nullizygosity for p53 Peer-reviewed

    H Nozawa, E Oda, K Nakao, M Ishihara, S Ueda, T Yokochi, K Ogasawara, Y Nakatsuru, S Shimizu, Y Ohira, K Hioki, S Aizawa, T Ishikawa, M Katsuki, T Muto, T Taniguchi, N Tanaka

    GENES & DEVELOPMENT　13　(10)　1240-1245　1999/05

    DOI： 10.1101/gad.13.10.1240 　

    ISSN： 0890-9369

  87. Transcription factor IRF-1 and its family members in the regulation of host defense Peer-reviewed

    T Taniguchi, N Tanaka, K Ogasawara, S Taki, M Sato, A Takaoka

    COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY　64　465-472　1999

    DOI： 10.1101/sqb.1999.64.465 　

    ISSN： 0091-7451

  88. Functional dissection of the cytoplasmic subregions of the IL-2 receptor βc chain in primary lymphocyte populations Peer-reviewed

    Hodaka Fujii, Kouetsu Ogasawara, Hidefumi Otsuka, Misao Suzuki, Ken-Ichi Yamamura, Taeko Yokochi, Tadaaki Miyazaki, Haruhiko Suzuki, Tak W. Mak, Shinsuke Taki, Tadatsugu Taniguchi

    EMBO Journal　17　(22)　6551-6557　1998/11/16

    ISSN： 0261-4189

  89. Requirement for IRF-1 in the microenvironment supporting development of natural killer cells (vol 391, pg 700, 1998)

    K Ogasawara, S Hida, N Azimi, Y Tagaya, T Sato, T Yokochi-Fukuda, TA Waldmann, T Taniguchi, S Taki

    NATURE　392　(6678)　843-843　1998/04

    ISSN： 0028-0836

  90. Involvement of NK1+ T cells and their IFN-γ production in the generalized Shwartzman reaction Peer-reviewed

    Kouetsu Ogasawara, Kazuyoshi Takeda, Wataru Hashimoto, Masayuki Satoh, Ryuhei Okuyama, Nobuaki Yanai, Masuo Obinata, Katsuo Kumagai, Haruhiko Takada, Hoshio Hiraide, Shuhji Seki

    Journal of Immunology　160　(7)　3522-3527　1998/04/01

    ISSN： 0022-1767

  91. Requirement for IRF-1 in the microenvironment supporting development of natural killer cells Peer-reviewed

    K. Ogasawara, S. Hida, N. Azimi, Y. Tagaya, T. Sato, T. Yokochi-Fukuda, T. A. Waldmann, T. Taniguchi, S. Taki

    Nature　391　(6668)　700-703　1998/02/12

    DOI： 10.1038/35636 　

    ISSN： 0028-0836

  92. Antimetastatic effect of NK1+ T cells on experimental haematogenous tumour metastases in the liver and lungs of mice Peer-reviewed

    S. Seki, W. Hashimoto, K. Ogasawara, M. Satoh, H. Watanabe, Y. Habu, H. Hiraide, K. Takeda

    Immunology　92　(4)　561-566　1997

    Publisher: Blackwell Publishing Ltd

    DOI： 10.1046/j.1365-2567.1997.00383.x 　

    ISSN： 0019-2805

  93. Multistage regulation of Th1-type immune responses by the transcription factor IRF-1 Peer-reviewed

    Shinsuke Taki, Takeo Sato, Kouetsu Ogasawara, Taeko Fukuda, Mitsuharu Sato, Shigeaki Hida, Gen Suzuki, Masao Mitsuyama, Eun-Hee Shin, Soumei Kojima, Tadatsugu Taniguchi, Yoshihiro Asano

    Immunity　6　(6)　673-679　1997

    Publisher: Cell Press

    DOI： 10.1016/S1074-7613(00)80443-4 　

    ISSN： 1074-7613

  94. Cytotoxic γδ or αβ T Cells with a Natural Killer Cell Marker, CD56, Induced from Human Peripheral Blood Lymphocytes by a Combination of IL-12 and IL-2 Peer-reviewed

    Masayuki Satoh, Shuhji Seki, Wataru Hashimoto, Kouetsu Ogasawara, Terutada Kobayashi, Katsuo Kumagai, Seiki Matsuno, Kazuyoshi Takeda

    Journal of Immunology　157　(9)　3886-3892　1996/11/01

    ISSN： 0022-1767

  95. Liver NK1.1+ CD4+ αβ T cells activated by IL-12 as a major effector in inhibition of experimental tumor metastasis Peer-reviewed

    Kazuyoshi Takeda, Shuhji Seid, Kouetsu Ogasawara, Ryoichi Anzai, Wataru Hashimoto, Keitaro Sugiura, Motoyoshi Takahashi, Masayuki Satoh, Katsuo Kumagai

    Journal of Immunology　156　(9)　3366-3373　1996/05/01

    ISSN： 0022-1767

  96. LPS induces NK1.1+ αβ T cells with potent cytotoxicity in the liver of mice via production of IL-12 from Kupffer cells Peer-reviewed

    Motoyoshi Takahashi, Kouetsu Ogasawara, Kazuyoshi Takeda, Wataru Hashimoto, Hiroshi Sakihara, Katsuo Kumagai, Ryoichi Anzai, Masayuki Satoh, Shuhji Seki

    Journal of Immunology　156　(7)　2436-2442　1996/04/01

    ISSN： 0022-1767

  97. Interleukin-12 induces cytotoxic NK1+ αβ T cells in the lungs of euthymic and athymic mice Peer-reviewed

    R. Anzai, S. Seki, K. Ogasawara, W. Hashimoto, K. Sugiura, M. Sato, K. Kumagai, K. Takeda

    Immunology　88　(1)　82-89　1996

    Publisher: Blackwell Publishing Ltd

    DOI： 10.1046/j.1365-2567.1996.d01-638.x 　

    ISSN： 0019-2805

  98. Cytotoxic NK1.1 Ag+ αβ T cells with intermediate TCR induced in the liver of mice by IL-12 Peer-reviewed

    W. Hashimoto, K. Takeda, R. Anzai, K. Ogasawara, H. Sakihara, K. Sugiura, S. Seki, K. Kumagai

    Journal of Immunology　154　(9)　4333-4340　1995

    ISSN： 0022-1767

Show all ︎Show first 5

Misc. 125

  1. 1J1-1 Performance comparison of cell phantoms developed for optical-resolution photoacoustic microscopy

    Nishimae Daisuke, Ishii Takuro, Ogasawara Koetsu, Saijo Yoshifumi

    Proceedings of Symposium on Ultrasonic Electronics　44　1　2023/11/13

    Publisher: Institute for Ultrasonic Elecronics

    DOI： 10.24492/use.44.0_1j1-1 　

    ISSN： 1348-8236

    eISSN： 2433-1910

  2. がん抗原免疫を軸とした治療抵抗性を克服するための遺伝子解析と基礎研究

    上田 翔平, 牛島 美保, 入江 厚, 千住 覚, 伊藤 甲雄, 浜名 洋, 岸 裕之, 小笠原 康悦, 宇高 恵子, 西村 泰治, 江藤 正俊

    西日本泌尿器科学会総会抄録集　75回　255-255　2023/11

    Publisher: (一社)西日本泌尿器科学会

  3. マウスモデルにおけるIn silico解析を用いた新規がん抗原の同定と難治性がんの克服

    上田 翔平, 牛島 美保, 入江 厚, 千住 覚, 伊藤 甲雄, 浜名 洋, 岸 裕之, 小笠原 康悦, 宇高 恵子, 江藤 正俊, 西村 泰治

    日本がん免疫学会総会プログラム・抄録集　27回　96-96　2023/06

    Publisher: 日本がん免疫学会

  4. Antibacterial Properties of TiO2 Layers Formed by Au-Sputtering and Thermal Oxidation of Titanium under Visible Light

    Ueda Takatoshi, Koizumi Ryusuke, Ueda Kyosuke, Ito Koyu, Ogasawara Kouetsu, Kanetaka Hiroyasu, Narushima Takayuki

    Materials Transactions (Web)　64　(1)　2023

    ISSN： 1347-5320

  5. 腫瘍抗原ワクチンにより増強される難治性がんに対する免疫チェックポイント阻害剤の抗腫瘍効果(Tumor antigen vaccine enhances anti-tumor effects of Immune checkpoint inhibitors against refractory cancers.)

    上田 翔平, 牛島 美保, 入江 厚, 千住 覚, 伊藤 甲雄, 浜名 洋, 岸 裕之, 小笠原 康悦, 宇高 恵子, 西村 泰治, 江藤 正俊

    西日本泌尿器科学会総会抄録集　74回　169-169　2022/11

    Publisher: (一社)西日本泌尿器科学会

  6. 腫瘍関連抗原の能動免疫による難治性がんの克服(Tumor associated antigen vaccine enhances anti-tumor effects of immune checkpoint inhibitors against refractory cancers.)

    上田 翔平, 牛島 美保, 入江 厚, 千住 覚, 伊藤 甲雄, 浜名 洋, 岸 裕幸, 小笠原 康悦, 宇高 恵子, 江藤 正俊, 西村 泰治

    日本癌学会総会記事　81回　J-1044　2022/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  7. マウスモデルにおけるIn silico解析を用いた新規がん抗原の同定と難治性がんの克服

    上田 翔平, 牛島 美保, 入江 厚, 千住 覚, 伊藤 甲雄, 浜名 洋, 岸 裕之, 小笠原 康悦, 宇高 恵子, 江藤 正俊, 西村 泰治

    日本がん免疫学会総会プログラム・抄録集　26回　77-77　2022/06

    Publisher: 日本がん免疫学会

  8. 金属アレルギーの発症機序と予防・治療法の確立に向けた免疫学的解析

    伊藤 甲雄, 小笠原 康悦

    臨床免疫・アレルギー科　76　(6)　668-674　2021/12

    Publisher: (有)科学評論社

    ISSN： 1881-1930

  9. マウスモデルにおける全遺伝子網羅的なIn silicoアルゴリズムによる新規がん抗原の同定と難治性膀胱がんの克服

    上田 翔平, 牛島 美保, 入江 厚, 千住 覚, 伊藤 甲雄, 浜名 洋, 岸 裕之, 小笠原 康悦, 宇高 恵子, 西村 泰治, 江藤 正俊

    西日本泌尿器科学会総会抄録集　73回　194-194　2021/11

    Publisher: (一社)西日本泌尿器科学会

  10. 腫瘍関連抗原の能動免疫と免疫チェックポイント阻害剤の併用による難治性がんの克服

    上田 翔平, 牛島 美保, 入江 厚, 千住 覚, 伊藤 甲雄, 浜名 洋, 小笠原 康悦, 岸 裕幸, 江藤 正俊, 西村 泰治

    日本癌学会総会記事　80回　[P12-3]　2021/09

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  11. 肺移植後閉塞性細気管支炎進展におけるNK細胞の役割 マウス異所性気管移植モデルを用いて

    川上 徹, 伊藤 甲雄, 松田 安史, 野田 雅史, 桜田 晃, 星川 康, 岡田 克典, 小笠原 康悦

    移植　55　(4)　462-462　2021/03

    Publisher: (一社)日本移植学会

    ISSN： 0578-7947

    eISSN： 2188-0034

  12. Enhancement of myogenic differentiation by MMP inhibition and attenuation of the enhancement effect in senescent cells

    Sakai Hiroki, Honda Takeshi, Ogasawara Kouetsu, Asagiri Masataka

    Proceedings for Annual Meeting of The Japanese Pharmacological Society　94　1-P2-LB47　2021

    Publisher: Japanese Pharmacological Society

    DOI： 10.1254/jpssuppl.94.0_1-p2-lb47 　

    eISSN： 2435-4953

    More details Close

    Extracellular matrix (ECM) and its regulatory proteins such as matrix metalloproteinases (MMPs) in skeletal muscles are inextricably linked with regulation of muscle mass and function. ECMs has also been found to be involved in muscle diseases such as muscular dystrophy and muscle mass loss like sarcopenia. We found that batimastat, a broad spectrum MMP inhibitor promotes myosin heavy chain (MHC) expression during myogenic differentiation of C2C12 myoblasts. The expression of myogenin, which is a transcription factor that induces MHC expression, was also enhanced by batimastat. Batimastat promoted MHC expression even under conditions where myogenic differentiation was strongly inhibited, such as differentiation into other cell type (osteoblast) or proliferative cultures. Increased MHC expression was found in other MMP inhibitor possessing similar spectrum to batimastat, but not in specific inhibitors for MMP-3, -9 or -13 in C2C12 myoblasts, suggesting that several MMPs coordinately regulate MHC expression. Interestingly, batimastat-induced enhancement of MHC expression was attenuated in myogenic differentiation of senescent C2C12 myoblasts with elevated senescence-associated β-galactosidase activity. These results indicate that the mechanism regulating myoblast differentiation by MMPs may be altered by cellular senescence. Elucidating the senescence-induced changes in MMP-mediated regulation can contribute to the understanding of the pathogenesis of age-related muscle diseases such as sarcopenia.

  13. マウス難治性癌モデルにおける腫瘍抗原ペプチドワクチン療法と免疫チェックポイント阻害剤の併用療法の奏効

    上田 翔平, 入江 厚, 千住 覚, 伊藤 甲雄, 浜名 洋, 小笠原 康悦, 岸 裕幸, 江藤 正俊, 西村 泰治

    日本癌学会総会記事　79回　OJ12-5　2020/10

    Publisher: (一社)日本癌学会

    ISSN： 0546-0476

  14. 口腔・全身のネットワーク～最先端研究-免疫・神経・内分泌～ アレルギーにおけるネオ・セルフ

    小笠原 康悦

    Journal of Oral Biosciences Supplement　2019　69-69　2019/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  15. 酸性細胞外pH馴化は造腫瘍性を促進する

    加藤 靖正, 鈴木 厚子, 前田 豊信, 小笠原 康悦

    日本生化学会大会プログラム・講演要旨集　92回　[1T09a-03]　2019/09

    Publisher: (公社)日本生化学会

  16. Au蒸着Ti基板の大気酸化によるAu添加TiO2膜の作製およびその可視光誘起抗菌能

    上田隆統志, 佐藤直生, 上田恭介, 伊藤甲雄, 小笠原康悦, 目代貴之, 金高弘恭, 成島尚之

    日本金属学会講演概要(CD-ROM)　164th　2019

    ISSN： 2433-3093

  17. Ti-Au合金表面に作製したAu添加TiO2膜の可視光照射下における抗菌能

    上田隆統志, 上田恭介, 伊藤甲雄, 小笠原康悦, 金高弘恭, 目代貴之, 目代貴之, 庭野吉己, 庭野吉己, 成島尚之

    日本防菌防黴学会年次大会要旨集　46th　2019

  18. 金属アレルギー研究の最前線

    小笠原 康悦

    東北矯正歯科学会雑誌　26　(1)　105-107　2018/12

    Publisher: 東北矯正歯科学会

    ISSN： 1340-2668

  19. 動物モデルを用いたパラジウムアレルギー発症の分子機構

    関 智水, 佐藤 直毅, 樋口 繁仁, 小笠原 康悦

    Journal of Oral Biosciences Supplement　2018　356-356　2018/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  20. 動物モデルを用いたパラジウムアレルギー発症の分子機構

    関 智水, 佐藤 直毅, 樋口 繁仁, 小笠原 康悦

    Journal of Oral Biosciences Supplement　2018　356-356　2018/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

  21. 大気酸化によりTi‐Au合金表面に作製したTiO2膜の可視光照射下における抗菌能およびラジカル生成

    上田隆統志, 上田恭介, 伊藤甲雄, 小笠原康悦, 目代貴之, 金高弘恭, 庭野吉己, 成島尚之

    日本金属学会講演概要(CD-ROM)　162nd　ROMBUNNO.217　2018/03/05

    ISSN： 1342-5730

  22. 大気酸化によりTi-Au合金表面に作製したTiO2膜の可視光照射下における抗菌能およびラジカル生成

    上田隆統志, 上田恭介, 伊藤甲雄, 小笠原康悦, 目代貴之, 金高弘恭, 庭野吉己, 成島尚之

    日本金属学会講演概要(CD-ROM)　162nd　2018

    ISSN： 2433-3093

  23. Ag含有非晶質リン酸カルシウムコーティングによる硬組織代替デバイスへの抗菌性と骨形成能の付与

    上田 恭介, 井上 紅花, 伊藤 甲雄, 小笠原 康悦, 成島 尚之

    日本骨形態計測学会雑誌　27　(1)　S140-S140　2017/05

    Publisher: 日本骨形態計測学会

    ISSN： 0917-4648

  24. 【金属と炎症】 マウスモデルを用いた歯科金属アレルギーの分子機構

    小笠原 康悦

    炎症と免疫　25　(2)　104-109　2017/02

    Publisher: (株)先端医学社

    ISSN： 0918-8371

  25. 金属に対するアレルギー反応の免疫化学的解析

    小笠原 康悦, 樋口 繁仁, 佐藤 直毅

    日本生化学会大会プログラム・講演要旨集　89回　[1F10-5]　2016/09

    Publisher: (公社)日本生化学会

  26. 金属記憶抗原提示細胞の同定

    武田 裕利, 小笠原 康悦, 井口 直彦, 高橋 哲

    日本口腔科学会雑誌　65　(2)　175-175　2016/07

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  27. 【Autoantigenとアレルギー疾患】 金属アレルギーと病原性T細胞

    武田 裕利, 井口 直彦, 山口 佳宏, 高橋 哲, 小笠原 康悦

    臨床免疫・アレルギー科　64　(3)　270-274　2015/09

    Publisher: (有)科学評論社

    ISSN： 1881-1930

  28. マーモセットのNK関連分子のクローニング

    小笠原 康悦

    Journal of Oral Biosciences Supplement　2015　154-154　2015/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  29. 【特殊なリンパ球群の最近の話題】 NK細胞死の分子機構

    中村 恭平, 小笠原 康悦

    炎症と免疫　23　(5)　388-393　2015/08

    Publisher: (株)先端医学社

    ISSN： 0918-8371

  30. パラジウムアレルギーにかかわるIFN-γ産生CD8+T細胞

    武田 裕利, 小笠原 康悦, 井口 直彦, 山口 佳宏, 高橋 哲

    日本口腔科学会雑誌　64　(2)　164-164　2015/07

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  31. 【免疫系とバイオマテリアル】 金属アレルギーを引き起こす病原性T細胞

    武田 裕利, 井口 直彦, 山口 佳宏, 高橋 哲, 小笠原 康悦

    バイオマテリアル-生体材料-　33　(2)　140-143　2015/04

    Publisher: 日本バイオマテリアル学会

    ISSN： 1347-7080

    eISSN： 2434-0359

  32. 超音波マルチモダリティーイメージング 炎症およびがんのイメージングを目的とした光音響効果増強の基礎的検討

    西條 芳文, 山崎 玲奈, 小笠原 康悦, 壹岐 伸彦, 田村 昂作

    超音波医学　42　(Suppl.)　S225-S225　2015/04

    Publisher: (公社)日本超音波医学会

    ISSN： 1346-1176

    eISSN： 1881-9311

  33. Cellular Labile Iron Activates NLRP3 Inflammasome

    Kyohei Nakamura, Tohru Fujiwara, Tomonori Ishii, Hideo Harigae, Kouetsu Ogasawara

    BLOOD　124　(21)　2014/12

    ISSN： 0006-4971

    eISSN： 1528-0020

  34. 【生体防御における免疫反応の新知見】 新展開を生じた生体反応の最新情報 細菌感染におけるペア型受容体の役割

    中山 勝文, 小笠原 康悦

    Surgery Frontier　21　(3)　273-277　2014/09

    Publisher: (株)メディカルレビュー社

    ISSN： 1340-5594

  35. 癌の分子標的治療から分子標的予防医学へ がんとNK細胞

    小笠原 康悦

    Journal of Oral Biosciences Supplement　2014　68-68　2014/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  36. ナノ粒子によるマクロファージ炎症の解析(1)

    古澤 慧美, 小笠原 康悦

    Journal of Oral Biosciences Supplement　2014　126-126　2014/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  37. ナノ粒子によるマクロファージ炎症の解析(2)

    小笠原 康悦, 古澤 慧美

    Journal of Oral Biosciences Supplement　2014　126-126　2014/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  38. 新規人工関節金属の生体内における金属溶出量の評価

    園淵 和明, 萩原 嘉廣, 金澤 憲治, 矢部 裕, 安藤 晃, 井樋 栄二, 小泉 雄一郎, 千葉 昌彦, 川野 光子, 小笠原 康悦

    日本整形外科学会雑誌　88　(8)　S1500-S1500　2014/08

    Publisher: (公社)日本整形外科学会

    ISSN： 0021-5325

  39. MHC class II dressed NK細胞によるT細胞の反応抑制

    小笠原 康悦

    臨床免疫・アレルギー科　61　(1)　112-118　2014/01

    Publisher: (有)科学評論社

    ISSN： 1881-1930

  40. Natural Killer Cell Death Mediated By NKG2D-Trogocytosis

    Kyohei Nakamura, Masafumi Nakayama, Mitsuko Kawano, Tomonori Ishii, Hideo Harigae, Kouetsu Ogasawara

    BLOOD　122　(21)　2013/11

    ISSN： 0006-4971

    eISSN： 1528-0020

  41. 【病態の理解に向かう アレルギー疾患研究-なぜ、アレルギーが起こるのか?発症・進展の新概念と臨床への展開】 (第3章)アレルギー疾患研究の最新トピック 金属アレルギー研究 動物モデルによる免疫学的解析

    川野 光子, 遠藤 美里, 佐藤 直毅, 鈴木 隆二, 小笠原 康悦

    実験医学　31　(17)　2823-2828　2013/11

    Publisher: (株)羊土社

    ISSN： 0288-5514

  42. 薬疹の理解に必要な免疫学 動物モデルを用いた金属アレルギーの研究

    小笠原 康悦, 川野 光子, 佐藤 直毅, 樋口 繁仁, 鈴木 隆二

    Journal of Environmental Dermatology and Cutaneous Allergology　7　(5)　361-361　2013/11

    Publisher: (一社)日本皮膚アレルギー・接触皮膚炎学会

    ISSN： 1882-0123

  43. レニンにより誘導されるNK細胞の免疫応答

    島田 栄理遣, 遠藤 実里, 小笠原 康悦

    Journal of Oral Biosciences Supplement　2013　130-130　2013/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  44. NK細胞の誘導性細胞死機構の発見

    小笠原 康悦, 島田 栄理遣, 遠藤 実里

    Journal of Oral Biosciences Supplement　2013　130-130　2013/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  45. 金属アレルギーにおける金属イオン可視化技術の開発

    遠藤 実里, 島田 栄理遣, 小笠原 康悦

    Journal of Oral Biosciences Supplement　2013　130-130　2013/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  46. 【新たな免疫抑制細胞】 免疫抑制にはたらくドレスNK細胞の発見

    橋元 亘, 中村 恭平, 遠藤 実里, 島田 栄理遣, 小笠原 康悦, 中山 勝文, 佐藤 直毅, 樋口 繁仁

    炎症と免疫　21　(4)　297-304　2013/06

    Publisher: (株)先端医学社

    ISSN： 0918-8371

  47. 内因性アトピーと金属アレルギー

    山口 隼人, 久保 利江子, 尾藤 利憲, 坂部 純一, 龍野 一樹, 平澤 典保, 小笠原 康悦, 戸倉 新樹

    アレルギー　62　(3-4)　455-455　2013/04

    Publisher: (一社)日本アレルギー学会

    DOI： 10.15036/arerugi.62.455_3 　

    ISSN： 0021-4884

    eISSN： 1347-7935

  48. Ubiquitin-proteasome-dependent degradation of CDC14A phosphatase via SPSB family of proteins

    Tadashi Nishiya, Ken Maruyama, Kazuma Matsumoto, Kouetsu Ogasawara, Souichi Miwa, Takashi Uehara

    JOURNAL OF PHARMACOLOGICAL SCIENCES　121　118P-118P　2013

    ISSN： 1347-8613

  49. 【新発見が続く自然リンパ球】 ドレスNK細胞による免疫抑制機能

    小笠原 康悦

    実験医学　30　(19)　3034-3039　2012/12

    Publisher: (株)羊土社

    ISSN： 0288-5514

  50. CDC14AはElongin B/C-Cul5-SPSB型E3ユビキチンリガーゼの基質である

    西屋 禎, 松本 一馬, 小笠原 康悦, 三輪 聡一, 上原 孝

    日本生化学会大会プログラム・講演要旨集　85回　3P-220　2012/12

    Publisher: (公社)日本生化学会

  51. MHC II dressed NK細胞

    小笠原 康悦

    感染・炎症・免疫　42　(3)　202-207　2012/10

    Publisher: 鳥居薬品(株)

    ISSN： 0387-1010

  52. 金属溶液の塗布による金属炎症モデルの構築

    高橋 亜希子, 小野 瑞穂, 土橋 明, 小笠原 康悦

    Journal of Oral Biosciences Supplement　2012　100-100　2012/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  53. リンパ球移植による金属アレルギー動物モデルの構築

    土橋 明, 高橋 亜希子, 小野 瑞穂, 小笠原 康悦

    Journal of Oral Biosciences Supplement　2012　100-100　2012/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  54. 金属と細菌成分による免疫細胞の反応性の検討

    小野 瑞穂, 土橋 明, 高橋 亜希子, 小笠原 康悦

    Journal of Oral Biosciences Supplement　2012　101-101　2012/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  55. ドレスNK細胞の発見

    小笠原 康悦, 小野 瑞穂, 土橋 明, 高橋 亜希子

    Journal of Oral Biosciences Supplement　2012　101-101　2012/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  56. NK細胞の生物学

    小笠原 康悦

    東北大学歯学雑誌　30　(2)　56-57　2011/12

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  57. T細胞機能調節 分子機能の観点から 新世界ザルにおけるT細胞受容体β鎖遺伝子のCDR3領域における正の選択(Increased positive selection pressure within the CDR regions of the T cell receptor beta gene in New World monkeys)

    北浦 一孝, 松谷 隆治, 藤井 克樹, 白井 顕治, 鈴木 さつき, 高崎 智彦, 小笠原 康悦, 西本 憲弘, 倉根 一郎, 鈴木 隆二

    日本免疫学会総会・学術集会記録　40　139-139　2011/11

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  58. Dermatological View 金属アレルギーの免疫学Up-to-date

    川野 光子, 小笠原 康悦

    Visual Dermatology　10　(11)　1208-1212　2011/10

    Publisher: (株)学研メディカル秀潤社

    ISSN： 2186-6589

  59. 金属アレルギーのT細胞解析

    小笠原 康悦

    Journal of Oral Biosciences　53　(Suppl.)　111-111　2011/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  60. 【金属アレルギー発症メカニズムと対策】 金属アレルギー動物モデルを用いた免疫学的解析

    小笠原 康悦

    臨床免疫・アレルギー科　55　(5)　530-534　2011/05

    Publisher: (有)科学評論社

    ISSN： 1881-1930

  61. NK細胞の生物学

    小笠原 康悦

    Dental Medicine Research　31　(1)　82-82　2011/03

    Publisher: 昭和大学・昭和歯学会

    ISSN： 1882-0719

  62. iNOS regulation by the SPRY domain- and SOCS box-containing proteins

    Tadashi Nishiya, Kazuma Matsumoto, Satoshi Maekawa, Takahiro Horinouchi, Masahiro Fujimuro, Kouetsu Ogasawara, Takashi Uehara, Soichi Miwa

    JOURNAL OF PHARMACOLOGICAL SCIENCES　115　102P-102P　2011

    ISSN： 1347-8613

  63. Elongin B/C、Cul5、Rbx2、及びSPRY domain-containing SOCS box protein(SSB)により構成されるE3ユビキチンリガーゼ複合体(ECS-SSB)は誘導型一酸化窒素合成酵素(iNOS)の分解制御システムである

    西屋 禎, 前川 聡, 松本 一馬, 藤室 雅弘, 小笠原 康悦, 上原 孝, 三輪 聡一

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　83回・33回　3T3-2　2010/12

    Publisher: (公社)日本生化学会

  64. 金属アレルギーの発症メカニズムと対策 マウス金属アレルギーモデルを用いた免疫学的解析

    小笠原 康悦, 鈴木 隆二

    アレルギー　59　(9-10)　1265-1265　2010/10

    Publisher: (一社)日本アレルギー学会

    DOI： 10.15036/arerugi.59.1265_1 　

    ISSN： 0021-4884

    eISSN： 1347-7935

  65. The lifetime of INOS is regulated by the SPRY domain-containing SOCS box protein family linking iNOS to the elongin BC-Cul5-Rbx2 E3 ubiquitin ligase complex

    Tadashi Nishiva, Satoshi Maekawa, Masahiro Fujimuro, Kouetsu Ogasawara, Takashi Uehara, Soichi Miwa

    NITRIC OXIDE-BIOLOGY AND CHEMISTRY　22　S11-S12　2010/06

    DOI： 10.1016/j.niox.2010.05.028 　

    ISSN： 1089-8603

  66. NK細胞の生物学

    小笠原 康悦

    東北大学歯学雑誌　29　(1)　1-11　2010/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  67. 生体防御と疾患

    小笠原 康悦

    東北医学雑誌　121　(2)　155-156　2009/12

    Publisher: 東北医学会

    ISSN： 0040-8700

  68. 高IgE外因性アトピー性皮膚炎(AD)と正常域IgE内因性ADの皮膚バリア能と知覚閾値の比較

    森 智子, 石田 耕一, 椋本 祥子, 山田 陽子, 芋川 玄爾, 椛島 健治, 小林 美和, 尾藤 利憲, 中村 元信, 小笠原 康悦, 戸倉 新樹

    Journal of Environmental Dermatology and Cutaneous Allergology　3　(4)　310-310　2009/10

    Publisher: (一社)日本皮膚アレルギー・接触皮膚炎学会

    ISSN： 1882-0123

  69. 金属アレルギーの発症にかかわる細胞集団の同定

    小笠原 康悦

    Journal of Oral Biosciences　51　(Suppl.)　72-72　2009/08

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  70. バイオマテリアルとアレルギー 金属アレルギー研究の最前線

    小笠原 康悦

    日本口腔インプラント学会誌　22　(特別号)　89-89　2009/08

    Publisher: (公社)日本口腔インプラント学会

    ISSN： 0914-6695

    eISSN： 2187-9117

  71. 自己免疫疾患におけるNKG2Dリガンドの異常発現の分子機構

    小笠原 康悦

    上原記念生命科学財団研究報告集　22　1-4　2008/12

    Publisher: (公財)上原記念生命科学財団

    eISSN： 2433-3441

  72. アレルギー 花粉症、皮膚炎症を中心にして 金属アレルギーマウスモデル用いた分子細胞生物学的病理解析

    川野 光子, 浦野 奈央子, 田中 和沙, 小笠原 康悦

    日本免疫学会総会・学術集会記録　38　49-49　2008/11

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  73. 腫瘍抗原および樹状細胞 腫瘍内浸潤マクロファージにおけるRAE-1の発現

    田中 和沙, 石崎, 川野 光子, 浦野 奈央子, 小笠原 康悦

    日本免疫学会総会・学術集会記録　38　107-107　2008/11

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  74. 金属アレルギーモデルマウスにおける耳介真皮へのT細胞の浸潤

    浦野 奈央子, 川野 光子, 田中 和沙, 崎, 岩崎 之克, 中村 雅典, 小笠原 康悦

    Journal of Oral Biosciences　50　(Suppl.)　135-135　2008/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  75. 【新時代の糖尿病学 病因・診断・治療研究の進歩】 糖尿病基礎研究の進歩 糖尿病と耐糖能低下の成因分類と発症機序 1型糖尿病 発症にかかわる免疫異常

    小笠原 康悦

    日本臨床　66　(増刊3 新時代の糖尿病学(1))　354-358　2008/05

    Publisher: (株)日本臨床社

    ISSN： 0047-1852

  76. 自己免疫疾患とNK活性化レセプター

    小笠原 康悦

    Frontiers in Rheumatology & Clinical Immunology　2　(1)　39-45　2008/02

    Publisher: (株)メディカルレビュー社

    ISSN： 1882-1472

  77. 生活習慣病における医学、薬学の萠芽的研究 糖尿病素因の発見のための新規診断法の開発

    小笠原 康悦

    医科学応用研究財団研究報告　25　187-190　2008/02

    Publisher: (公財)鈴木謙三記念医科学応用研究財団

    ISSN： 0914-5117

    eISSN： 2185-2561

  78. NKG2DリガンドRAE-1 epsilonの転写制御解析

    田中 和沙, 崎, 浦野 奈央子, 小笠原 康悦

    日本免疫学会総会・学術集会記録　37　211-211　2007/10

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  79. 【巧妙な免疫調節システム NK細胞の細胞認識機構と疾患 癌・自己免疫疾患・拒絶反応への関与】 NK細胞の制御シグナルと疾患

    小笠原 康悦

    実験医学　25　(9)　1282-1286　2007/06

    Publisher: (株)羊土社

    ISSN： 0288-5514

  80. 【巧妙な免疫調節システム NK細胞の細胞認識機構と疾患 癌・自己免疫疾患・拒絶反応への関与】 NK細胞活性型レセプターNKG2Dの生体内における機能 腫瘍、自己免疫疾患、骨髄移植

    石崎 和沙, 小笠原 康悦

    実験医学　25　(9)　1321-1325　2007/06

    Publisher: (株)羊土社

    ISSN： 0288-5514

  81. 「感染・免疫研究のフロンティア 注目の若手研究者」の企画にあたって 免疫系におけるNKレセプターの役割

    小笠原 康悦

    Journal of Oral Biosciences　48　(Suppl.)　73-73　2006/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  82. 自己免疫性糖尿病におけるNK活性化レセプターNKG2D

    小笠原 康悦, 藤原 成芳

    感染・炎症・免疫　36　(2)　164-166　2006/06

    Publisher: 鳥居薬品(株)

    ISSN： 0387-1010

  83. NKレセプターNKG2Dと自己免疫性糖尿病 (病気と免疫)

    小笠原 康悦, 藤原 成芳

    免疫　2006　301-307　2006

    Publisher: 中山書店

    ISSN： 0918-6557

  84. 骨髄移植拒絶に関わるNK細胞の標的分子の発見

    小笠原 康悦

    細胞工学　24　(12)　1312-1313　2005/11

    Publisher: (株)学研メディカル秀潤社

    ISSN： 0287-3796

  85. HIV由来VPRの細胞侵入現象とMHCクラスIへの抗原提示メカニズムの解明

    藤原 成芳, 石坂 幸人, 小笠原 康悦

    日本免疫学会総会・学術集会記録　35　129-129　2005/11

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  86. NKG2D BLOCKADE PREVENTS AUTOIMMUNE DIABETES IN NOD MICE

    OGASAWARA Kouetsu, HAMERMAN Jessica A., EHRLICH Lauren R., BOUR-JORDAN Helene, SANTAMARIA Pere, BLUESTONE Jeffrey A., LANIER Lewis L.

    52　27-27　2005/10/01

    ISSN： 0918-8959

  87. 【1型糖尿病 成因と治療の最近の進歩】 1型糖尿病におけるNK活性化レセプターを介した膵β細胞障害機構

    小笠原 康悦

    内分泌・糖尿病科　20　(5)　444-451　2005/05

    Publisher: (有)科学評論社

    ISSN： 1341-3724

  88. NK,NKT NKG2Dとそのリガンドの機能と役割

    小笠原 康悦

    Annual Review免疫　2005　102-109　2004/12

    Publisher: (株)中外医学社

  89. NK活性化レセプターNKG2Dとそのリガンドの機能と役割

    小笠原 康悦, Bluestone Jeffrey A, Lanier Lewis L

    日本免疫学会総会・学術集会記録　34　332-332　2004/11

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  90. 新規NK細胞レセプターとそのリガンドの同定

    白鳥 行大, 小笠原 康悦, 斉藤 隆, Lanier Lewis, 荒瀬 尚

    日本癌学会総会記事　63回　436-436　2004/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  91. 感染・免疫 NK細胞活性化レセプター,NKG2Dの機能と役割

    小笠原 康悦

    Journal of Oral Biosciences　46　(5)　366-366　2004/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  92. がん細胞はどのようにしてNK細胞からの攻撃を逃れているのか?

    小笠原 康悦

    Journal of Nippon Medical School　71　(3)　222-222　2004/06

    Publisher: 日本医科大学医学会

    ISSN： 1345-4676

    eISSN： 1347-3409

  93. 新規NK細胞レセプターとそのリガンドのクローニング及び機能解明

    白鳥 行大, 小笠原 康悦, 海野 緑, 坂元 亜矢子, Lanier Lewis, 斉藤 隆, 荒瀬 尚

    日本免疫学会総会・学術集会記録　33　143-143　2003/11

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  94. 慢性関節リウマチ骨破壊における破骨細胞の形成機構と制御に関する研究 T細胞による破骨細胞分化制御と関節炎性骨破壊の抑制に関する研究

    高柳 広, 谷口 維紹, 小笠原 康悦, 肥田 重明, 佐藤 浩二郎, 高岡 晃教, 田中 啓二, 千葉 智樹, 村田 茂穂, 織田 弘美, 中村 耕三

    短期プロジェクト研究報告書 骨の破壊と老化　(97〜99年)　57-2　2001/03

    Publisher: (財)東京都高齢者研究・福祉振興財団 東京都老人総合研究所

  95. CD8(+) T cell-mediated skin disease in mice lacking IRF-2, the transcriptional attenuator of interferon-alpha/beta signaling

    S Hida, K Ogasawara, K Sato, M Abe, H Takayanagi, T Yokochi, T Sato, S Hirose, T Shirai, S Taki, T Taniguchi

    IMMUNITY　13　(5)　643-655　2000/11

    ISSN： 1074-7613

  96. マウス移植片対宿主反応早期における遺伝子発現の解析

    涌井 昌俊, 山口 晃弘, 小笠原 康悦, 横地 妙子, 唐木 幸子, 土屋 尚之, 池田 康夫, 徳永 勝士

    日本免疫学会総会・学術集会記録　30　144-144　2000/11

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  97. 転写因子IRF-2によるtype I-IFNシグナル依存性,非依存性Th1/2細胞分化制御

    肥田 重明, 佐藤 健夫, 小笠原 康悦, 佐藤 浩二郎, 高柳 広, 横地 妙子, 竹田 潔, 審良 静男, 谷口 維紹, 瀧 伸介

    日本免疫学会総会・学術集会記録　30　234-234　2000/11

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  98. TNF-related apoptosis-inducing ligand(TRAIL)がウイルス感染時に果たす役割の検討

    佐藤 浩二郎, 小笠原 康悦, 肥田 重明, 高柳 広, 榧垣 伸彦, 八木田 秀雄, 奥村 康, 田中 信之, 谷口 維紹

    日本免疫学会総会・学術集会記録　30　324-324　2000/11

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  99. NK,CD8+T細胞の制御とIRFファミリー転写因子

    谷口 維紹, 小笠原 康悦

    日本癌学会総会記事　59回　223-223　2000/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  100. Transcription factor IRF-1 is required for the activation of NK cells by IL-12 and allogenic bone marrow rejection

    K Ogasawara, K Sato, S Hida, H Takayanagi, T Yokochi, S Taki, T Taniguchi

    FASEB JOURNAL　14　(6)　A1022-A1022　2000/04

    ISSN： 0892-6638

  101. CD8+ T cell-mediated skin disease in mice lacking IRF-2, the transcriptional attenuator of interferon-α/β signalling

    Hida, S, Ogasawara, K, Sato, K, Abe, M, Takayanagi, H, Yokochi, T, Sato, T, Hirose, S, Shirai, S, Taki, S, Taniguchi, T

    Immunity　13　643-655　2000

  102. NK細胞・NKT細胞 IL-15によるNK細胞の分化,活性化とそのシグナル

    小笠原 康悦

    Annual Review免疫　2000　146-154　1999/12

    Publisher: (株)中外医学社

  103. Analysis of gene expression profile in murine graft-versus-host reaction using differential display method.

    M Wakui, A Yamaguchi, D Sakurai, K Ogasawara, T Yokochi, T Taniguchi, N Tsuchiya, Y Ikeda, K Tokunaga

    BLOOD　94　(10)　333B-333B　1999/11

    ISSN： 0006-4971

  104. Type I IFNによるNK細胞の活性化機構の解析

    小笠原 康悦, 佐藤 浩二郎, 肥田 重明, 横地 妙子, 瀧 伸介, 谷口 維紹

    日本免疫学会総会・学術集会記録　29　272-272　1999/10

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  105. I型インターフェロン(type I IFN)によるTRAILの転写制御の機構及びその生理的意義の解析

    佐藤 浩二郎, 小笠原 康悦, 肥田 重明, 横地 妙子, 瀧 伸介, 榧垣 伸彦, 竹田 和由, 八木田 秀雄, 奥村 康, 田中 信之

    日本免疫学会総会・学術集会記録　29　340-340　1999/10

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  106. I型インターフェロン(IFN)系の制御の欠損による免疫異常と炎症性皮膚疾患

    肥田 重明, 小笠原 康悦, 佐藤 浩二郎, 佐藤 健夫, 横地 妙子, 田中 信之, 瀧 伸介, 谷口 維紹

    日本免疫学会総会・学術集会記録　29　199-199　1999/10

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  107. 【臨床遺伝子学'99 免疫研究の最前線】 免疫応答における細胞内情報伝達機構 免疫系におけるIRFの役割

    小笠原 康悦

    最新医学　54　(増刊)　2210-2219　1999/09

    Publisher: (株)最新医学社

    ISSN： 0370-8241

  108. 【NK細胞:最近の話題】 IRF-1,IL-15とNK細胞分化

    小笠原 康悦

    炎症と免疫　7　(5)　503-511　1999/08

    Publisher: (株)先端医学社

    ISSN： 0918-8371

  109. IRFファミリー転写因子による免疫系の調節

    瀧 伸介, 佐藤 健夫, 小笠原 康悦, 肥田 重明

    阿蘇シンポジウム記録　22回　97-110　1999/07

    Publisher: (株)南山堂

  110. 【NK細胞をめぐって】 NK細胞の発生分化とサイトカイン

    小笠原 康悦

    臨床免疫　31　(1)　1-6　1999/01

    Publisher: (有)科学評論社

    ISSN： 0386-9695

  111. Mechanism of the tumor suppression by transcription factor IRF-1

    ODA Eri, NOZAWA Hiroaki, NAKAO Kazuki, UEDA Seiji, YOKOCHI Taeko, ODASAWARA Koetsu, NAKATSURU Yoko, SHIMIZU Seiichiro, OHIRA Yoshikazu, ISHIKAWA Takatoshi, AIZAWA Shinichi, KATSUKI Motoya, MUTO Tetsuichiro, TANIGUCHI Tadatsugu, TANAKA Nobuyuki

    21　516-516　1998/12/01

  112. 転写因子IRF-1によるNK細胞の分化制御機構

    小笠原 康悦

    免疫Immunology Frontier　8　(6)　370-374　1998/12

    Publisher: (株)メディカルレビュー社

    ISSN： 0917-0774

  113. IRF-1遺伝子欠損マウスにおけるNK-T細胞の機能解析

    小笠原 康悦, 肥田 重明, 横地 妙子, 佐藤 健夫, 谷口 維紹, 瀧 伸介

    日本臨床免疫学会会誌　(26回抄録集)　167-167　1998/10

    Publisher: 日本臨床免疫学会

    ISSN： 0911-4300

    eISSN： 1349-7413

  114. 転写抑制因子IRF-2は転写活性化因子IRF-1とは異なる機構でTh1分化に寄与する

    佐藤 健夫, 肥田 重明, 小笠原 康悦, 横地 妙子, 谷口 維紹, 瀧 伸介

    日本臨床免疫学会会誌　(26回抄録集)　62-62　1998/10

    Publisher: 日本臨床免疫学会

    ISSN： 0911-4300

    eISSN： 1349-7413

  115. I型インターフェロン(IFN)系の抑制性転写因子の欠損による炎症性皮膚疾患の発症と免疫異常

    肥田 重明, 阿部 雅明, 小笠原 康悦, 佐藤 健夫, 横地 妙子, 広瀬 幸子, 白井 俊一, 谷口 維紹, 瀧 伸介

    日本臨床免疫学会会誌　(26回抄録集)　155-155　1998/10

    Publisher: 日本臨床免疫学会

    ISSN： 0911-4300

    eISSN： 1349-7413

  116. 【発生工学を用いた免疫担当細胞の機能解析】 NK細胞の分化におけるIRF-1とIL-15の機能

    肥田 重明, 小笠原 康悦, 瀧 伸介

    組織培養工学　24　(9)　346-349　1998/08

    Publisher: (株)ニュー・サイエンス社

    ISSN： 1344-1027

  117. 【免疫1998-99】 免疫系の制御とその異常 NK細胞の分化における転写因子IRF-1の役割

    小笠原 康悦, 瀧 伸介

    Molecular Medicine　35　(臨増 免疫1998-99号)　166-174　1998/08

    Publisher: (株)中山書店

    ISSN： 0918-6557

  118. 細菌内毒素によるTNK細胞の活性化とガンマーインターフェロン産生誘導

    小笠原 康悦

    東北大学歯学雑誌　16　(1)　1-10　1997/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  119. Interleukin-12 as an inducer of cytotoxic effectors in anti-tumor immunity

    Katsuo Kumagai, Kazuyoshi Takeda, Wataru Hashimoto, Shuji Seki, Kouetsu Ogasawara, Ryoichi Anzai, Motoyoshi Takahashi, Masayuki Sato, Hidemi Rikjishi

    International Reviews of Immunology　14　(2-3)　229-256　1997

    Publisher: Informa Healthcare

    DOI： 10.3109/08830189709116854 　

    ISSN： 0883-0185

  120. SCIDmiceにおいてmIL-12により活性化されるリンパ球の同定と実験的転移の抑制

    小笠原 康悦

    日本癌学会総会記事　54回　433-433　1995/09

    Publisher: 日本癌学会

    ISSN： 0546-0476

  121. 転移抑制効果をもつTNK細胞の性状と胸腺外分化の可能性

    小笠原 康悦

    日本癌学会総会記事　53回　415-415　1994/10

    Publisher: 日本癌学会

    ISSN： 0546-0476

  122. 細胞接着分子の基礎と臨床 癌転移と接着分子 CD44を中心として

    小笠原 康悦, 熊谷 勝男

    Oncology & Chemotherapy　10　(1)　22-27　1994/02

    Publisher: 日本婦人科悪性腫瘍化学療法学会

    ISSN： 0913-9834

  123. マウス腫瘍RL♂1の実験的転移に対するmIL-12の投与効果

    安西 良一, 杉浦 慶太郎, 橋元 亘, 崎原 浩, 小笠原 康悦, 下田 明, 竹田 和由, 力石 秀実, 熊谷 勝男

    日本免疫学会総会・学術集会記録　23　167-167　1993/10

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

  124. 癌の転移と細胞接着分子 肺転移を制御する接着分子CD44

    力石 秀実, 小笠原 康悦, 熊谷 勝男

    実験医学　11　(16)　2176-2181　1993/10

    Publisher: (株)羊土社

    ISSN： 0288-5514

  125. 癌細胞のGM-CSF遺伝子発現と肺転移能(1) B16melanomaへのGM-CSF遺伝子の導入とその転移形成性の検討

    小笠原 康悦

    日本細菌学雑誌　48　(2)　443-443　1993/03

    Publisher: 日本細菌学会

    ISSN： 0021-4930

    eISSN： 1882-4110

Show all ︎Show first 5

Industrial Property Rights 12

  1. T細胞受容体の認識機構を用いたがん又は感染症の治療及び診断

    小笠原 康悦

    特許第7012364号

    Property Type: Patent

  2. 遺伝子特異的非バイアス増幅法

    小笠原 康悦

    特許第6793956号

    Property Type: Patent

  3. 金属高感度・高感受性の金属アレルギー動物モデルの樹立

    小笠原 康悦

    特許第6666001号

    Property Type: Patent

  4. NKG2Dの調節

    ラニア ルイス エル., 小笠原 康悦, ブルーストーン ジェフリー エー.

    特許第5693127号

    Property Type: Patent

  5. NKG2Dの調節

    ラニア ルイス エル., 小笠原 康悦, ブルーストーン ジェフリー エー.

    特許第4667451号

    Property Type: Patent

  6. 抗原特異的MHC発現調節法

    小笠原 康悦

    Property Type: Patent

  7. T細胞受容体の認識機構を用いたがん又は感染症の治療及び診断

    小笠原 康悦

    Property Type: Patent

  8. 遺伝子特異的非バイアス増幅法

    小笠原 康悦

    Property Type: Patent

  9. NKG2Dの調節

    ラニア ルイス エル., 小笠原 康悦, ブルーストーン ジェフリー エー.

    Property Type: Patent

  10. 光触媒機能性部材およびその製造方法

    成島 尚之, 上田 恭介, 上田 隆統志, 佐渡 翔太, 小笠原 康悦, 伊藤 甲雄, 金高 弘恭

    Property Type: Patent

  11. 金属高感度・高感受性の金属アレルギー動物モデルの樹立

    小笠原 康悦

    Property Type: Patent

  12. NKG2Dの調節

    ラニア ルイス エル., 小笠原 康悦, ブルーストーン ジェフリー エー.

    Property Type: Patent

Show all Show first 5

Research Projects 32

  1. 多機能可溶型TCR-NK療法の開発

    小笠原 康悦

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(開拓)

    Institution: 東北大学

    2025/06/27 - 2029/03/31

  2. メラトニン代謝産物AMKによる脳内炎症制御に着目した新たな認知症予防基盤の構築

    渡辺 数基, 小笠原 康悦, 平山 順, 渡辺 靖章, 服部 淳彦

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2025/04/01 - 2029/03/31

  3. Development of photo-functional antibacterial and antiviral material surfaces considering bacterial adhesion and virus adsorption

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2025/04/01 - 2028/03/31

  4. 歯周疾患による金属アレルギー悪化の機序の解明

    小笠原 康悦

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2025/04/01 - 2028/03/31

  5. Development of antibacterial-expressing self-maintenance implant surfaces using biological window

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2024/04/01 - 2028/03/31

  6. がん特異的T細胞受容体を用いた診断・治療法開発

    小笠原 康悦

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2023/06/30 - 2025/03/31

  7. Analysis of the mechanism of autoimmune disease using murine model

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: University of Tsukuba

    2022/04/01 - 2025/03/31

  8. 金属アレルギー病原性ペプチドの探索研究

    小笠原 康悦

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

    More details Close

    歯科は、生体金属材料を最も使用している診療科である。生体金属材料は、患者のQOLの向上に役立ってきているが、その一方で、金属アレルギーを引き起こすことが報告されており、金属アレルギーの病態解明が求められている。金属アレルギーは、遅延型過敏反応でⅣ型アレルギーに分類されT細胞依存性の免疫疾患とされている。研究代表者は、これまで、歯科金属で広く用いられているパラジウムを対象に金属アレルギー動物モデルを開発して、パラジウムに反応するT細胞の特定、およびT細胞受容体の特定を進めてきた。本研究では、金属アレルギーを引き起こす抗原に着目して、金属アレルギー病原性ペプチドの探索研究を行うことを目的としている。本年度は、病原性ペプチドが提示されると考えられる主要組織適合抗原複合体（MHC）について研究を進めた。マウス抗原提示細胞を用いて培養し、パラジウム溶液を添加した群と添加しない群について、培養をおこなったものの、想定したような細胞増殖が認められなかった。MHCに提示されたペプチドを、質量分析装置で解析するためには、相当量のタンパク質が必要で、そのためには、多数の細胞が必要である。パラジウム溶液添加により、予想に反して細胞の増殖が抑制されたことから、質量分析を行うには工夫が必要であることが判明した。現在、マウス骨髄細胞を分化誘導して用いているが、マウス骨髄細胞数を増やす、あるいは、マウス樹状細胞株を用いるなどして、この問題を解決する予定である。

  9. 金属アレルギー病原性ペプチドの探索研究

    小笠原 康悦

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

  10. Analysis of the mechanism of autoimmune disease using murine model

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: University of Tsukuba

    2022/04 - 2025/03

  11. Materials surface design to inactivate coronaviruses by focusing on spike proteins

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2021/04 - 2025/03

  12. Functionalization of implant by bio-active coating with ion release and high bonding strength

    UEDA Kyosuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2020/04/01 - 2024/03/31

    More details Close

    A coating process of bio-dissolvable bioactive glass with antibacterial elements was developed to provide bone compatibility and antibacterial properties to dental implants made of Ti. The coating films were prepared on the mirror-finished abutment by RF magnetron sputtering and, on the screw-shaped and roughened root by a sol-gel dip method. The coating films had high adhesion, dissolved in simulated body fluid, and the release of antimicrobial elements was detected, indicating antibacterial activity.

  13. Basic research for new immunological therapies based on a pathological mechanism of periodontal disease

    Tanaka Yoshihiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Fukuoka Dental College

    2020/04/01 - 2023/03/31

    More details Close

    Periodontal disease is an infection caused by periodontal pathogenic bacteria and has attracted attention as being caused by the helper T cell Th17, but the detailed mechanism of immune response was unknown. When periodontal pathogenic bacteria were introduced into the intestines of mice at the amount that periodontal disease patients swallow daily, it was found that periodontal pathogenic bacteria were taken up from the Peyer's patches of the intestines and that Th17 cells that respond to periodontal pathogenic bacteria (responsible Th17 cells) were activated in the intestines. Subsequently, we elucidated that the responsible Th17 cells migrate from the gut to the mouth, which is infected with periodontal pathogenic bacteria, causing severe periodontal disease. Meanwhile, in mice without intestinal bacteria, the responsible Th17 cells were not activated and periodontal disease did not occur, indicating that intestinal bacteria were involved in the development of periodontal disease.

  14. Challenge to paradigm shift in head and neck cancer treatment

    Ogasawara Koetsu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2019/06/28 - 2023/03/31

    More details Close

    In immunotherapy, immune checkpoint inhibitors have been effective to tumor, but the response rate is about 20%. Therefore, more effective treatment has been desired. This requires a tumor-specific immune response. Using the third-generation T cell receptor repertoire analysis technology developed by the applicants, I aimed to establish a foundation for a new tumor immunotherapy using tumor-specific T cell receptors. Using a soluble form of tumor-specific T cell receptor, binding to target cells was investigated. Tumor-specific T cell receptors could bind to target cell specifically. This method may lead to the development of new therapy using tumor-specific T cell receptor.

  15. Studies on new diagnosis and treatments of metal allergy

    Ogasawara Kouetsu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

    More details Close

    We elucidated the molecular mechanism of the onset of palladium allergy. In vitro culture condition, we found that when antigen-presenting cells were added with a palladium solution, MHC class I expression was reduced and re-expressed on the antigen-presenting cells. It was also found that the antigenic peptide was changed in the process of expression reduction and recovery of MHC class I, and was replaced with a peptide that should not be normally expressed. Furthermore, it was clarified that allergic antigens are expressed and allergic T cells are activated by antigen-peptide substitution with palladium.

  16. Creation of optically-functional titanium surface with compatibility of bone forming ability and antibacterial activity and its application to implants

    Narushima Takayuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2018/04 - 2022/03

    More details Close

    We succeeded in fabricating a Ti surface with both osteogenic and antibacterial properties for application to dental implants. The Au-containing TiO2 layers prepared on the Ti surface by an original process based on Au-sputtering and thermal oxidation showed antibacterial activity against E. coli under visible-light irradiation. The photofunction of the Au-containing TiO2 layers obtained in this study is considered to be an achievement to realize the “self-maintenance function” in which the implant itself plays a role in “healing” by reattaching to bone tissue and also enables “prevention” by patients themselves. These results, together with the excellent adhesion between the TiO2 layers and the Ti substrates, are expected to be applied not only to dental implants but also to the prevention of contact infection of the new coronavirus (SARS-CoV-2).

  17. Generation mechanism of Neo-self by metal / chemical

    Kouetsu Ogasawara

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: Tohoku University

    2016/06/30 - 2021/03/31

    More details Close

    In this study, we investigated the molecular mechanism by which the antigen peptide-MHC complex modified by haptens as “neo-self”. To explore the mechanisms, we analyzed T cell receptors that react with haptens such as metal. We identified the metal allergy-specific T cell receptors in a mouse model of metal allergy using palladium (Pd). Furthermore, by in vitro cell culture system, we found that the antigen peptide-MHC complex was changed by Pd solution and the expression of MHC was transiently reduced. In other words, we found that the behavior of the antigen peptide-MHC complex induces changes in the repertoire of T cell receptors. Therefore, our findings suggest that the behavior of antigen peptide-MHC complex may be induce the neo-self.

  18. Development of a new rheumatoid arthritis monitoring method by analysis of anti-CCP antibody-producing B cell repertoire

    ISHII Tomonori

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2017/04/01 - 2020/03/31

    More details Close

    Abatacept was administered to 15 patients with rheumatoid arthritis who did not lose their activity despite existing treatment. Before treatment, peripheral blood was collected from patients and lymphocytes were separated from peripheral blood. Repertoire analysis of B cell receptor and T cell receptor was performed on the obtained lymphocytes. Abatacept was treated for more than three months in these patients. After treatment, a second repertoire analysis of B cells and T cells was performed and compared with the repertoire before treatment. Abatacept treatment was effective in 70% of patients. Specific T cell repertoire decreased before and after treatment in lymphocytes obtained from patients who responded to these treatments

  19. Establish the foundation for advanced tumor immunotherapy

    Ogasawara Koetsu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2017/06 - 2020/03

    More details Close

    Although immune checkpoint inhibitors have been effective against intractable tumors, we hope more effective treatments. Therefore, we performed a study to detect tumor-specific T cell receptors. When mouse peripheral tissues around tumors and lymphoid tissues were collected using a tumor-bearing mouse model, T cell accumulation was observed around the tumors. In addition, it was possible to detect T cell receptors that are considered to be tumor-specific in lymphoid tissues around the tumor. Furthermore, we were able to establish a comprehensive analysis method for T cell receptors.

  20. ネオ・セルフ Competitive

    小笠原 康悦

    System: 新学術領域

    2016 - 2020

  21. Immunochemical analysis of dental metal allergy

    OGASAWARA KOUETSU, ITO KOYU

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2016/04/01 - 2019/03/31

    More details Close

    Metal allergy is considered to be delayed-type of allergy by T-cell dependent diseases, but its immune mechanism is not well understood. In this study, we performed to clarify the characteristics of T cells involved in the onset of metal allergy using the our technology. We found that non-biased gene amplification plays a very important role in T cell receptor analysis. Thus, in T cell receptor analysis, it is necessary to use a non-biased gene amplification method, such as a single chain adapter ligation PCR by our technology.

  22. Pathogenesis of inflammation or allergy in dental metal material

    OGASAWARA KOUETSU, NAKAYAMA MASAFUMI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

    More details Close

    Metal is used as a component of biomaterials in dental care, because metal has stiffness, elasticity, and ductility. In dental care, the metal is frequently used as a part of denture, an implant and restoration. However, the molecular mechanisms underlying metal inflammation and metal allergy have not been understood. In this study, we examined the molecular mechanism of development for metal allergy and inflammation by focusing NK cells and NK receptors. We found that NKG2D, a NK activating receptor, is expressed on CD8+T cells are involved in metal allergy.

  23. Development of effective diagnostic methods and treatment of oral cancer and pre-cancerous lesions

    Hashimoto Wataru, OGASAWARA KOUETSU, TANIGUCHI TAKAHIRO

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2012/04/01 - 2016/03/31

    More details Close

    To develop effective diagnostic methods and treatment of oral cancer, NKG2D-ligand expression on human oral cancer cell lines was analysed by a flowcytometer. As a result, the only weak expression of NKG2D-ligand was determined on two tumor cell lines. In addition, the expression of NKG2D-ligand was analysed by immunohistochemical methods on normal cells, the epithelial dysplasia cells and squamous carcinoma cells, no expression was observed on all type cells. On the other hand, the high expression of PD-1 ligands, which are immune control receptors, was determined on two tumor cell lines by a flowcytometric analysis. In the future, we will check the PD-1 ligand expression in the past of pathological tissue specimens. These results suggested that PD-1 ligands, but not NKG2D ligands, will be new marker for diagnosis of oral cancer.

  24. Biofunctionalization of metallic biomaterials by forming ceramic interface layers

    NARUSHIMA Takayuki, KASUGA Toshihiro, OGASAWARA Kouetsu, UEDA Kyosuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2013/04 - 2016/03

    More details Close

    Ceramic coating layers of amorphous calcium phosphate (ACP) and TiO2 were prepared on Ti, and their antibacterial activities were evaluated. Ag-containing ACP layers fabricated by RF magnetron sputtering exhibited antibacterial activity through the continuous release of Ag ions, caused by the resorbability of ACP. TiO2 layers fabricated by thermal oxidation processes showed photodegradation of organic compounds and antibacterial activity under both UV- and visible-light irradiation. The introduction of Au into TiO2 layers from Ti-Au alloy substrates contributed to the expression of visible-light response. The optimized fabrication processes provided the coating films with both the bone forming ability and antibacterial activity.

  25. Photoacoustic molecular imaging of macrophage with metal nanoparticle

    SAIJO YOSHIFUMI, OGASAWARA Koetsu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04 - 2015/03

    More details Close

    Acoustical-resolution and optical-resolution photoacoustic microscope system were developed. Photoacoustic properties of some metals was measured and macrophage phagocytosing gold nanorod was observed by the system. Photoacoustic imaging of macrophage with gold nanorod has a potential to be a biomarker of inflammation.

  26. Macrophage inflammatory responses to amorphous silica particles

    NAKAYAMA Masafumi, OGASAWARA Kouetsu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2012/04 - 2015/03

    More details Close

    Although amorphous silica particles are used in a wide variety of products such as pharmaceuticals and foods, the immunotoxicity of these particles is not fully understood. In this study, we addressed the relationship between the size of amorphous silica and the inflammatory activity. Of note, 30 nm-1000 nm diameter silica particles induced macrophage lysosomal destabilization, cell death, and IL-1β secretion at markedly higher levels than did 3000 nm-10000 nm silica particles. Consistent with in vitro results, intra-tracheal administration of 30 nm silica particles into mice caused more severe lung inflammation than that of 3000 nm silica particles. Taken together, these results suggest that silica particle size impacts immune responses, with submicron amorphous silica particles inducing higher inflammatory responses than silica particles over 1000 nm in size.

  27. Application of Super High-Purity Iron for Dental Treatment

    WATANABE Makoto, OGASAWARA Kouetsu, TSUBOI Akito

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

    More details Close

    Dental metal has increased the patient's QOL as a repair material. However, safer alternative materials are desired, because there is a possibility that several oral disease may be caused by dental metal. The "ultra-high purity iron (UHP-Fe)" that is the next-generation material unparalleled in the world has been generated in the Institute for Materials Research at Tohoku University. We also developed the novel safety test for evaluating biomaterials using experimental animal model. In this study, our purpose is to examine whether UHP-Fe is valuable for safer biomaterials in the clinical application using the safety test in vivo. We injected the wire of UHP-Fe into the mice subcutaneously, Five days later we obtained the surrounding tissue embedded in the metal wire. To investigate the elution of metal ions in the in vivo, we were dissolved the surrounding tissue and the solution was analyzed by mass spectrum analysis metal ions quantitative analysis technique (ICP-MS method). In addition, the removed wires were detected the corrosion of metal surfaces by scanning electron microscopy (SEM method). There is almost no corrosion of the surface ultra-high purity iron and inflammation of the subcutaneous tissue was not observed. Therefore, ultra-high purity iron is a safe biological material, suggesting that it will become a new medical biomaterial in the future.

  28. To explore new biomarkers in autoimmune diseases

    OGASAWARA Kouetsu, ISHII Tomonori

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

    More details Close

    Autoimmune disease is considered to be caused by genetic factors, environmental factors, and the time factor. However, the diagnostic methods including environmental factors and a time factor have not been established. NK cells are known as a group of cells of the innate immune system. NK cell has been examined cell surface molecules and receptors in the view of the elimination of tumor and infection defense. Therefore, until now it has been unknown whether NK receptors and NK cells are involved in autoimmune disease. In this study, we performed experiments on the basis of the original idea by which NK receptors and NK cells are involved in autoimmune diseases. Our purpose was to explore new biomarkers of autoimmune diseases, such as systemic lupus erythematosus (SLE) and type I diabetes for a diagnostic index.In this study we found that NKG2D ligands that do not express almost normal tissues isabnormally expressed in autoimmune disease model mice, and autoreactive T cells had abnormally express NKG2D. Therefore, these results suggest that NKG2D ligand isavailable as a new biomarker in type I diabetes.

  29. Imminological analysis of inflammation by dental materials

    OGASAWARA Kouetsu, SASAKI Keiichi, NAKAYAMA Masafumi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2010 - 2012

    More details Close

    In recent years, a lot of medical biomaterials have been developed and have been widely used. Metal is used as a component of biomaterials in dental treatment, because metal is easy to process and metal has stiffness, elasticity, and ductility. In dentistry, the metal is frequently used as a part of denture, an implant and crown restoration. Also, it is utilized as vascular stents and artificial joint material in medical area. However, since oral disease, inflammation and allergic dermatitis are caused by metal, the risk has been pointed out previously. Recently, patients of allergic dermatitis or inflammation, which are caused by metal are increased, because the frequency of use of metal material by improving medical and dental technology is increased, and people to put the ornaments such as necklaces and earrings are increased. However, the pathogenesis of the disease induced by the metal is not well understood.In this study, we addressed to develop new diagnostic methods for delayed-type hypersensitivity and inflammation induced by metal using the experimental animal model and we examined the molecular mechanism of development for metal allergy and inflammation.In lymphocyte stimulation test using the patient's peripheral blood for diagnostic metal allergy, we found that the sensitivity is enhanced by inhibition of CTLA-4. In this method, we may develop new method of lymphocyte stimulation test for diagnostic metal allergy. In a mouse model metal allergy, we were able to develop a new animal model ofmetal allergy which is closer to the human pathology.

  30. 発がんによるNKG2Dリガンドの発現機構の解明(特定領域がん(発がん))

    小笠原 康悦

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 特定領域研究

    Institution: 国立病院医療センター(臨床研究部)

    2006 - 2007

    More details Close

    生体防御機構は,発がんした細胞を排除すべく免疫監視に当たっている。しかしながら,がん細胞はImmuno-editingと呼ばれる,巧妙に生体防御系から逃れている。我々は,生体防御の最前線で働くNK細胞,特にNK活性化レセプターNKG2Dに着目し研究を進めてきた。興味深いことに,NKG2Dリガンドは,正常組織ではサイレントであり,発現がみとめられないが,がん細胞,特に悪性度の高い腫瘍には広範にかつ恒常的にNKG2Dリガンドの異常発現が認められる。我々は,発がんによるNKG2Dリガンドの異常発現の分子機構を解明することを目的とし,研究を行った。 我々は,NKG2Dリガンドのがん細胞における発現調節機構を解明するため,プロモーターのクローニングおよびその解析を進めた。RAE-1 d,eを強く発現しているマウス肺がん細胞であるLL/2を用い,mRNAを調整し5'RACE法によりRAE-1 d,eのmRNAの配列を決定した。その結果,NCBIで公開されているdatabaseでは報告されていなかった新規のexonがRAE-1eにおいて存在することが判明した。このmRNA情報をもとに転写開始点を決定し,その上流のプロモーターと思われる領域をクローニングした。RAE-1eのプロモーター解析により,Sp1,NF-kB,AP-1の結合領域が存在することが明らかとなった。さらに,Sp1,NF-kB,AP-1に対するケミカルインヒビターによる解析で,これら転写因子が,肺がん細胞LL/2においてRAE-1eの発現にかかわっており,特にSp1が重要であることが判明した。さらに,siRNAによるSp1のknockdownにより,RAE-1eの発現が抑制された。以上のことより,がん細胞におけるRAE-1 eの発現は,Sp1が重要な転写因子であることが明らかとなった.

  31. Contribution of NK cells and NK related molecules in autoimmunity

    OGASAWARA Kouetsu, FUJIWARA Naruyoshi, TANAKA Kazusa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Research Institute, International Medical Center of Japan

    2006 - 2007

    More details Close

    Autoimmune diseases are caused by abnormal immune responses in self-tissues. Co-stimulatory molecules play important roles in immune responses. We focused the signaling mechanisms of co-stimulatory molecules and their ligands to explore development of autoimmune diseases. Type I diabetes is an autoimmune disease in which insulin-producing beta cells in pancreatic islets are destroyed by auto-reactive T cells. We showed that autoreactive T cells infiltrating the pancreas in diabetic NOD mice express NKG2D and NKG2D ligands are present in the pre-diabetic pancreas islets. Treatment with anti-NKG2D monoclonal antibody during the pre-diabetic stage completely prevented disease by impairing expansion of autoreactive T cells and blocking their effector functions. However, the molecular mechanisms by which RAE-1, one of NKG2D ligands are expressed in the pancreas in the development diabetes are unknown. We found that RAE-1 expression is regulated by transcriptional factors and that RAE-1 alpha is a major NKG2D ligand in NOD. In addition, transcriptional activator (s) derived from NOD may regulate RAE-1 alpha expression.

  32. NK細胞、NK-T細胞における転写因子IRFファミリーによる制御機構の解明

    小笠原 康悦

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 東京大学

    1999 - 2000

Show all Show first 5