Details of the Researcher

PHOTO

Emi Kanno
Section
Graduate School of Medicine
Job title
Professor
Degree

  • 博士（医学）（東北大学）

  • 修士（看護学）（山形大学）

e-Rad No.
10431595

Research History 6

  • 2022/06 - Present
    東北大学大学院医学系研究科 看護技術開発学分野　教授

  • 2018/11 - 2022/05
    東北大学大学院医学系研究科保健学専攻 准教授

  • 2013/04 - 2018/10
    東北大学大学院医学系研究科保健学専攻

  • 2007/04 - 2013/03
    東北大学大学院医学系研究科保健学専攻　助教

  • 2006/04 - 2007/03
    Tohoku University　School of Health Sciences, School of Medicine, Faculty of Medicine

  • 2003/04 - 2006/03
    東北大学病院　看護部　看護師

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Education 3

  • Tohoku University　Graduate School, Division of Medical Sciences

    2007/04 - 2011/03

  • Yamagata University　Graduate School, Division of Medical Sciences　看護学専攻

    2001/04 - 2003/03

  • Yamagata University　Faculty of Medicine　看護学科

    1997/04 - 2001/03

Committee Memberships 6

  • 看護理工学会　(査読委員)

    2018 - Present

  • 日本創傷・オストミ－・失禁管理学会　(評議員)

    2017 - Present

  • 日本創傷治癒学会　(評議員・学会賞選考委員会委員・将来構想委員会委員)

    2015 - Present

  • 日本褥瘡学会　(評議員・利益相反委員)

    2013 - Present

  • 公益社団法人 日本看護科学学会　(若手研究者活動推進委員会委員・和文誌専任査読委員・Scoping Review "insulin ball" メンバー)

    2021/07 -

  • 日本褥瘡学会東北地方会　(事務局長・世話人)

    2021/04 -

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Professional Memberships 10

  • JAPAN ACADEMY OF NURSING SCIENCE

  • 日本創傷・オストミ－・失禁管理学会

  • THE JAPANESE SOCIETY FOR MEDICAL MYCOLOGY

  • 日本創傷治癒学会(2007/08-)

  • Japanese Society of Nursing Art and Science

  • THE JAPANESE SOCIETY FOR IMMUNOLOGY

  • 日本生体防御学会(2010/07-)

  • CO-MEDICAL RESEARCH SOCIETY OF STRUCTURE AND FUNCTION

  • 北日本看護学会(2001/04-)

  • JAPANESE SOCIETY OF PRESSURE ULCERS

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Research Interests 10

  • 乳酸菌

  • 看護技術学

  • 基礎看護学

  • ケアイノベーション

  • pattern-recognition receptor: PRR

  • neutrophilic inflammatory responses

  • 免疫

  • 褥瘡、難治性皮膚潰瘍

  • 細菌感染、真菌感染

  • 創傷治癒、感染症、皮膚組織、褥瘡、慢性創傷、免疫

Research Areas 3

  • Life sciences / Infectious disease /

  • Life sciences / Basic nursing /

  • Life sciences / Plastic and reconstructive surgery /

Awards 9

  1. 【サムライインキュベート賞】

    2022/03　2021年度 仙台市「SENDAI NEW PUBLIC」

  2. 【サムライインキュベート賞】

    2021/05　2020年度 仙台市「SENDAI NEW PUBLIC」

  3. 【研究奨励賞】

    2020/11　第50回日本創傷治癒学会　Dectin-2シグナルを介した皮膚創傷治癒へのNatural Killer T細胞欠損の影響

  4. 【学会奨励賞】

    2019/09　コ・メディカル形態機能学会第18回学術集会　乳酸菌Enterococcus faecalis局所投与による皮膚創傷治癒の促進

  5. 【平成30年度 医学部・医学系研究科教育貢献賞】

    2019/03　東北大学大学院医学系研究科

  6. 【最優秀演題賞】

    2018/09　第20回日本褥瘡学会学術集会　緑膿菌接種創の治癒過程と菌排除におけるNKT細胞欠損の影響

  7. 【研究奨励賞】

    2016/12　第46回日本創傷治癒学会　マウス皮膚創傷治癒過程におけるCARD9を介したシグナル伝達の影響

  8. 【ポスター賞】

    2015　第17回日本褥瘡学会学術集会　褥瘡に対する局所陰圧閉鎖療法施行時における創傷治癒と栄養状態に関する考察

  9. 【ポスター演題優秀賞】

    2014/12　第44回日本創傷治癒学会　緑膿菌が創部アポトーシス誘導に与える影響

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Papers 95

  1. CARD9-mediated macrophage responses and collagen fiber capsule formation caused by textured-type breast implants. International-journal

    Miki Shoji, Emi Kanno, Hiromasa Tanno, Kenji Yamaguchi, Sinyo Ishi, Naoyuki Takagi, Shiho Kurosaka, Ko Sato, Momoko Niiyama, Akihiko Ito, Keiko Ishii, Yoshimichi Imai, Kazuyoshi Kawakami, Masahiro Tachi

    Plastic and reconstructive surgery　2023/10/17

    DOI： 10.1097/PRS.0000000000011152 　

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    BACKGROUND: An increasing number of women are undergoing breast implantation for cosmetic purposes and for reconstructive purposes after breast excision. The surface morphology of the breast implant is one of the key factors associated with the induction of capsule contraction. The effect of surface morphology on the inflammatory response following implant insertion remains unclear, however. This study conducted comparative analyses to determine the effect of the textured and smooth surface morphology of silicone sheets. METHODS: Each type of silicone sheet was inserted into the subcutaneous pocket below the panniculus carnosus in C57BL/6 mice and mice with genetic disruption of CARD9, Dectin-1, Dectin-2, or Mincle. We also analyzed the collagen fiber capsule thickness, histological findings, and macrophage inflammatory response, including TGF-β synthesis. RESULTS: We found that textured surface morphology contributed to the formation of collagen fiber capsules and the accumulation of fibroblasts and myofibroblasts, and was accompanied by the accumulation of TGF-β-expressing macrophages and foreign-body giant cells. CARD9 deficiency attenuated collagen fiber capsule formation, macrophage responses, and TGF-β synthesis, although the responsible C-type lectin receptors (CLRs) remain to be clarified. CONCLUSIONS: These results suggest that CARD9 may have a strong impact on silicone sheet insertion through the regulation of macrophage responses.

  2. Cutaneous wound healing promoted by topical administration of heat-killed Lactobacillus plantarum KB131 and possible contribution of CARD9-mediated signaling. International-journal

    Shinyo Ishi, Emi Kanno, Hiromasa Tanno, Shiho Kurosaka, Miki Shoji, Toshiro Imai, Kenji Yamaguchi, Kanna Kotsugai, Momoko Niiyama, Haruko Kurachi, Fuko Makabe, Takumi Watanabe, Ko Sato, Keiko Ishii, Hiromitsu Hara, Yoshimichi Imai, Kazuyoshi Kawakami

    Scientific reports　13　(1)　15917-15917　2023/09/23

    DOI： 10.1038/s41598-023-42919-z 　

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    Optimal conditions for wound healing require a smooth transition from the early stage of inflammation to proliferation, and during this time alternatively activated (M2) macrophages play a central role. Recently, heat-killed lactic acid bacteria (LAB), such as Lactobacillus plantarum (L. plantarum) have been reported as possible modulators affecting the immune responses in wound healing. However, how signaling molecules regulate this process after the administration of heat-killed LAB remains unclear. In this study, we examined the effect of heat-killed L. plantarum KB131 (KB131) administration on wound healing and the contribution of CARD9, which is an essential signaling adaptor molecule for NF-kB activation upon triggering through C-type lectin receptors, in the effects of this bacterium. We analyzed wound closure, histological findings, and inflammatory responses. We found that administration of KB131 accelerated wound closure, re-epithelialization, granulation area, CD31-positive vessels, and α-SMA-positive myofibroblast accumulated area, as well as the local infiltration of leukocytes. In particular, M2 macrophages were increased, in parallel with CCL5 synthesis. The acceleration of wound healing responses by KB131 was canceled in CARD9-knockout mice. These results indicate that the topical administration of KB131 accelerates wound healing, accompanying increased M2 macrophages, which suggests that CARD9 may be involved in these responses.

  3. Distinct roles for Dectin-1 and Dectin-2 in skin wound healing and neutrophilic inflammatory responses. International-journal Peer-reviewed

    Kenji Yamaguchi, Emi Kanno, Hiromasa Tanno, Ayako Sasaki, Yuki Kitai, Takayuki Miura, Naoyuki Takagi, Miki Shoji, Jun Kasamatsu, Ko Sato, Yuka Sato, Momoko Niiyama, Yuka Goto, Keiko Ishii, Yoshimichi Imai, Shinobu Saijo, Yoichiro Iwakura, Masahiro Tachi, Kazuyoshi Kawakami

    The Journal of investigative dermatology　141　(1)　164-176　2020/06/05

    DOI： 10.1016/j.jid.2020.04.030 　

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    C-type lectin receptors (CLRs) recognize microbial polysaccharides. The CLRs Dectin-1 and Dectin-2, which are triggered by β-glucan and α-mannan, respectively, contribute to up-regulation of the inflammatory response. Recently, we demonstrated that activation of the Dectin-2 signal delayed wound healing; in previous studies, triggering the Dectin-1 signal promoted this response. However, the precise roles of these CLRs in skin wound healing remain unclear. This study was conducted to determine the roles of Dectin-1 and Dectin-2 in skin wound healing, with a particular focus on the kinetics of neutrophilic inflammatory response. Full-thickness wounds were created on the backs of C57BL/6 mice, and the effects of Dectin-1 or Dectin-2 deficiency and those of β-glucan or α-mannan administration were examined. We also analyzed wound closure, histological findings, and neutrophilic inflammatory response including neutrophil extracellular trap (NET) formation at the wound sites. We found that Dectin-1 contributed to the acceleration of wound healing by inducing early-phase neutrophil accumulation, whereas Dectin-2 was involved in prolonged neutrophilic responses and NET formation, leading to delayed wound healing. Dectin-2 deficiency also improved collagen deposition and TGF-β1 expression. These results suggest that Dectin-1 and Dectin-2 have different roles in wound healing through their different effects on the neutrophilic response.

  4. Batroxobin promotes wound healing after burn injury by enhancing blood flow. International-journal

    Toshiro Imai, Yuki Sato, Hiromasa Tanno, Wakana Kamada, Shinyo Ishi, Miki Shoji, Hiromu Matsunaga, Ko Sato, Yoshimichi Imai, Emi Kanno

    Plastic and reconstructive surgery　2025/04/01

    DOI： 10.1097/PRS.0000000000012137 　

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    BACKGROUND: In the process of burn wound healing, promoting healing and suppressing wound progression by enhancing blood flow is considered essential. Various agents that increase blood flow are currently being assessed. Batroxobin (DF-521; Defibrase®) is a denitrogenating agent extracted from Bothrops moojeni, used as a thrombin-like serine protease to improve ischemic conditions. However, it remains unclear how this agent affects the burn wound healing process. In this study, we conducted analyses to define the effects of batroxobin administration on burn wound healing. METHODS: Full-thickness burn wounds were created on the dorsal skin of C57BL/6 mice by applying a 90℃, 5-mm-diameter soldering iron for 10 s. Immediately after wounding, batroxobin (30 batroxobin units/kg/mouse) was administered intraperitoneally daily. As a vehicle control, the same volume of saline was administered. We analyzed wound area, histological findings, blood flow, growth factor and chemokine synthesis, and ischemia-reperfusion related factor expression. RESULTS: We found that the systemic administration of batroxobin prevented burn wound progression, which was accompanied by decreased expression of TNF-α and NOX2 and reduced synthesis of Hif-1α. In addition, this agent promoted wound healing by enhancing blood flow, increasing S100A4-positive fibroblast accumulation, and stimulating the production of growth factors (bFGF, EGF, and PlGF). CONCLUSIONS: These results indicate that the systemic administration of batroxobin prevented burn wound progression and accelerated the healing process by enhancing blood flow. (Word count: 222).

  5. Contribution of CARD9 signaling to wound healing in skin promoted by topical administration of heat-killed Enterococcus faecalis strain KH2 and the involvement of Dectin-2. International-journal

    Shiho Kurosaka, Hiromasa Tanno, Minako Hirose, Wakana Kamada, Rena Takayashiki, Ikue Sone, Yuki Sato, Takumi Watanabe, Shinyo Ishi, Miki Shoji, Yoshimichi Imai, Ko Sato, Keiko Ishii, Hiromitsu Hara, Sho Yamasaki, Shinobu Saijo, Yoichiro Iwakura, Kazuyoshi Kawakami, Emi Kanno

    Frontiers in immunology　16　1550934-1550934　2025

    DOI： 10.3389/fimmu.2025.1550934 　

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    INTRODUCTION: Lactic acid bacteria (LAB) are well known for their beneficial effects on the regulation of immune responses and host protection against microbial infections. We previously reported that heat-killed Enterococcus faecalis strain KH2 (heat-killed KH2), a species of LAB, enhances inflammatory responses at wound sites and accelerates the skin wound healing process. In this study, we aimed to clarify the pathway underlying the wound-healing effects of heat-killed KH2. We focused on CARD9, a common adaptor molecule for C-type lectin receptors and Dectin-2, the upstream receptor for this adaptor molecule. METHODS: Four full-thickness dermal wounds were created on the backs of wild-type (WT) mice, CARD9 KO mice, and Dectin-2 KO mice, and the effects of heat-killed KH2 administration were examined. We analyzed the percent wound closure, re-epithelialization, granulation tissue formation, and the production of inflammatory cytokines and chemokines. RESULTS: Heat-killed KH2 administration enhanced wound closure, granulation tissue formation, and re-epithelialization in WT mice. However, these effects were absent in heat-killed KH2-treated CARD9 KO mice. Similar results were observed in the migration of neutrophils and the production of TNF-α, IL-6, KC, and MIP-2 in heat-killed KH2-treated CARD9 KO mice. Furthermore, heat-killed KH-2 induced activation of reporter cells expressing Dectin-2. Finally, heat-killed KH-2 treatment in Dectin-2 KO mice did not promote skin wound healing. CONCLUSION: These results suggest that recognition of heat-killed KH2 by Dectin-2 may activate CARD9-mediated signaling, which may contribute to the promotion of skin wound healing through KH2 treatment.

  6. Innate phase production of IFN-γ by memory and effector T cells expressing early activation marker CD69 during infection with Cryptococcus deneoformans in the lungs. International-journal

    Anna Miyahara, Aya Umeki, Ko Sato, Toshiki Nomura, Hideki Yamamoto, Tomomitsu Miyasaka, Daiki Tanno, Ikumi Matsumoto, Tong Zong, Takafumi Kagesawa, Akiho Oniyama, Kotone Kawamura, Xiaoliang Yuan, Rin Yokoyama, Yuki Kitai, Emi Kanno, Hiromasa Tanno, Hiromitsu Hara, Sho Yamasaki, Shinobu Saijo, Yoichiro Iwakura, Keiko Ishii, Kazuyoshi Kawakami

    Infection and immunity　e0002424　2024/05/03

    DOI： 10.1128/iai.00024-24 　

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    Cryptococcus deneoformans is a yeast-type fungus that causes fatal meningoencephalitis in immunocompromised patients and evades phagocytic cell elimination through an escape mechanism. Memory T (Tm) cells play a central role in preventing the reactivation of this fungal pathogen. Among these cells, tissue-resident memory T (TRM) cells quickly respond to locally invaded pathogens. This study analyzes the kinetics of effector T (Teff) cells and Tm cells in the lungs after cryptococcal infection. Emphasis is placed on the kinetics and cytokine expression of TRM cells in the early phase of infection. CD4+ Tm cells exhibited a rapid increase by day 3, peaked at day 7, and then either maintained their levels or exhibited a slight decrease until day 56. In contrast, CD8+ Tm cells reached their peak on day 3 and thereafter decreased up to day 56 post-infection. These Tm cells were predominantly composed of CD69+ TRM cells and CD69+ CD103+ TRM cells. Disruption of the CARD9 gene resulted in reduced accumulation of these TRM cells and diminished interferon (IFN) -γ expression in TRM cells. TRM cells were derived from T cells with T cell receptors non-specific to ovalbumin in OT-II mice during cryptococcal infection. In addition, TRM cells exhibited varied behavior in different tissues. These results underscore the importance of T cells, which produce IFN-γ in the lungs during the early stage of infection, in providing early protection against cryptococcal infection through CARD9 signaling.

  7. Differences in clinicopathological characteristics between lipohypertrophy and localized insulin-derived amyloidosis: A scoping review. International-journal

    Kanae Mukai, Hiromasa Tanno, Junko Sugama, Toshihiko Yanagita, Emi Kanno

    Chronic diseases and translational medicine　10　(1)　22-30　2024/03

    DOI： 10.1002/cdt3.98 　

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    Insulin is used as a therapeutic agent in patients with diabetes, and cutaneous lipohypertrophy (LH) and localized insulin-derived amyloidosis (LIDA) are well-known adverse effects associated with insulin injections. The clinical implications, management, assessment methods, and pathological differentiation of LH and LIDA have been recently updated. This review was to update our knowledge of the pathological differentiation, effects of insulin absorption, hypoglycemic events, and recent assessment methods for LH and LIDA. A scoping review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta Analyses extension for Scoping Reviews guidelines. Original studies and case reports in English were also included. PubMed and Scopus databases were searched for keywords to identify papers published up to January 2022. A total of 113 studies were identified through a database search, and 31 were eligible for inclusion in this scoping review. In the 31 studies included in this review, patients with type 2 diabetes had high frequencies of LH and LIDA. LH outcome parameters were assessed using pathological findings and imaging. LIDA is mainly determined by pathological methods, such as hematoxylin and eosin and Congo red staining. Several in vitro and in vivo LIDA models of LIDA have been developed. These results suggest that pathological analysis is required to identify LH and LIDA. It is important to consider LIDA, as it likely influences insulin adsorption and glycemic control. Although several studies have evaluated the LIDA process, little is known about the mechanisms underlying the development of adverse effects associated with insulin injections.

  8. 創傷治癒へのC型レクチン受容体の関与

    丹野 寛大, 菅野 恵美

    BIO Clinica　37　(9)　870-873　2022/08

    Publisher: (株)北隆館

    ISSN： 0919-8237

  9. 【急性期褥瘡のアセスメントとケア-慢性化・重症化を防ぐために-】(Part 2)急性期褥瘡の特徴

    丹野 寛大, 菅野 恵美

    看護技術　68　(8)　736-742　2022/07

    Publisher: (株)メヂカルフレンド社

    ISSN： 0449-752X

  10. Deficiency of lung-specific claudin-18 leads to aggravated infection with Cryptococcus deneoformans through dysregulation of the microenvironment in lungs

    Ko Sato, Ikumi Matsumoto, Koya Suzuki, Atsushi Tamura, Aki Shiraishi, Hiroshi Kiyonari, Jun Kasamatsu, Hideki Yamamoto, Tomomitsu Miyasaka, Daiki Tanno, Anna Miyahara, Tong Zong, Takafumi Kagesawa, Akiho Oniyama, Kotone Kawamura, Yuki Kitai, Aya Umeki, Emi Kanno, Hiromasa Tanno, Keiko Ishii, Sachiko Tsukita, Kazuyoshi Kawakami

    Scientific Reports　11　(1)　2021/12

    DOI： 10.1038/s41598-021-00708-6 　

    eISSN： 2045-2322

  11. Topical administration of heat-killed enterococcus faecalis strain kh2 promotes re-epithelialization and granulation tissue formation during skin wound-healing

    Hiromasa Tanno, Emi Kanno, Shiho Kurosaka, Yukari Oikawa, Takumi Watanabe, Ko Sato, Jun Kasamatsu, Tomomitsu Miyasaka, Shinyo Ishi, Miki Shoji, Naoyuki Takagi, Yoshimichi Imai, Keiko Ishii, Masahiro Tachi, Kazuyoshi Kawakami

    Biomedicines　9　(11)　2021/11

    DOI： 10.3390/biomedicines9111520 　

    eISSN： 2227-9059

  12. Role of Dectin-2 in the phagocytosis of Cryptococcus neoformans by dendritic cells. International-journal

    Yuki Kitai, Ko Sato, Daiki Tanno, Xiaoliang Yuan, Aya Umeki, Jun Kasamatsu, Emi Kanno, Hiromasa Tanno, Hiromitsu Hara, Sho Yamasaki, Shinobu Saijo, Yoichiro Iwakura, Keiko Ishii, Kazuyoshi Kawakami

    Infection and immunity　89　(10)　IAI0033021　2021/07/12

    DOI： 10.1128/IAI.00330-21 　

    ISSN： 0019-9567

    eISSN： 1098-5522

  13. Contribution of Invariant Natural Killer T Cells to the Clearance of Pseudomonas aeruginosa from Skin Wounds. International-journal

    Hiromasa Tanno, Emi Kanno, Suzuna Sato, Yu Asao, Mizuki Shimono, Shiho Kurosaka, Yukari Oikawa, Shinyo Ishi, Miki Shoji, Ko Sato, Jun Kasamatsu, Tomomitsu Miyasaka, Hideki Yamamoto, Keiko Ishii, Yoshimichi Imai, Masahiro Tachi, Kazuyoshi Kawakami

    International journal of molecular sciences　22　(8)　2021/04/10

    DOI： 10.3390/ijms22083931 　

    ISSN： 1661-6596

    eISSN： 1422-0067

  14. 【創傷における感染対策〜この10年で何が変わったか〜】創傷における感染防御機構、影響を与える要因

    丹野 寛大, 菅野 恵美

    WOC Nursing　9　(4)　4-11　2021/04

    Publisher: (株)医学出版

  15. 【創傷における感染対策〜この10年で何が変わったか〜】Wound hygiene(ウンドハイジーン)の概念

    菅野 恵美, 丹野 寛大

    WOC Nursing　9　(4)　12-17　2021/04

    Publisher: (株)医学出版

  16. Dectin-2-mediated initiation of immune responses caused by influenza virus hemagglutinin.

    Hideki Yamamoto, Chikako Tomiyama, Ko Sato, Jun Kasamatsu, Kazuki Takano, Aya Umeki, Nana Nakahata, Tomomitsu Miyasaka, Emi Kanno, Hiromasa Tanno, Sho Yamasaki, Shinobu Saijo, Yoichiro Iwakura, Keiko Ishii, Kazuyoshi Kawakami

    Biomedical research (Tokyo, Japan)　42　(2)　53-66　2021

    DOI： 10.2220/biomedres.42.53 　

    ISSN： 0388-6107

    eISSN： 1880-313X

  17. Effect of CARD9 Deficiency on Neutrophil-Mediated Host Defense against Pulmonary Infection with Streptococcus pneumoniae. International-journal

    Shigenari Ishizuka, Rin Yokoyama, Ko Sato, Ryuhei Shiroma, Ayako Nakahira, Hideki Yamamoto, Kazuki Takano, Takafumi Kagesawa, Tomomitsu Miyasaka, Jun Kasamatsu, Emi Kanno, Hiromasa Tanno, Keiko Ishii, Kazuyoshi Kawakami

    Infection and immunity　89　(1)　2020/12/15

    DOI： 10.1128/IAI.00305-20 　

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    Streptococcus pneumoniae is a major causative bacterium of community-acquired pneumonia. Dendritic cell-associated C-type lectin-2 (dectin-2), one of the C-type lectin receptors (CLRs), was previously reported to play a pivotal role in host defense against pneumococcal infection through regulating phagocytosis by neutrophils while not being involved in neutrophil accumulation. In the present study, to elucidate the possible contribution of other CLRs to neutrophil accumulation, we examined the role of caspase recruitment domain-containing protein 9 (CARD9), a common adaptor molecule for signal transduction triggered by CLRs, in neutrophilic inflammatory response against pneumococcal infection. Wild-type (WT), CARD9 knockout (KO), and dectin-2 KO mice were infected intratracheally with pneumococcus, and the infected lungs were histopathologically analyzed to assess neutrophil accumulation at 24 h postinfection. Bronchoalveolar lavage fluids (BALFs) were collected at the same time point to count the neutrophils and assess the production of inflammatory cytokines and chemokines. Neutrophil accumulation was significantly decreased in CARD9 KO mice, but not in dectin-2 KO mice. Tumor necrosis factor alpha (TNF-α), keratinocyte-derived chemokine (KC), and macrophage inflammatory protein-2 (MIP-2) production in BALFs were also attenuated in CARD9 KO mice, but not in dectin-2 KO mice. Production of TNF-α and KC by alveolar macrophages stimulated with pneumococcal culture supernatants was significantly attenuated in CARD9 KO mice, but not in dectin-2 KO mice, compared to that in each group's respective control mice. In addition, pneumococcus-infected CARD9 KO mice showed larger bacterial burdens in the lungs than did WT mice. These data indicate that CARD9 is required for neutrophil migration after pneumococcal infection, as well as inflammatory cytokine and chemokine production by alveolar macrophages, and suggest that a CLR distinct from dectin-2 may be involved in this response.

  18. Limited Role of Mincle in the Host Defense against Infection with Cryptococcus deneoformans. International-journal

    Yuki Sato, Ko Sato, Hideki Yamamoto, Jun Kasamatsu, Tomomitsu Miyasaka, Daiki Tanno, Anna Miyahara, Takafumi Kagesawa, Akiho Oniyama, Kotone Kawamura, Rin Yokoyama, Yuki Kitai, Aya Umeki, Shigenari Ishizuka, Kazuki Takano, Ryuhei Shiroma, Nana Nakahata, Kaori Kawakami, Emi Kanno, Hiromasa Tanno, Sho Yamasaki, Hiromitsu Hara, Keiko Ishii, Kazuyoshi Kawakami

    Infection and immunity　88　(11)　2020/10/19

    DOI： 10.1128/IAI.00400-20 　

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    Cryptococcus deneoformans is an opportunistic fungal pathogen that frequently causes fatal meningoencephalitis in patients with impaired cell-mediated immune responses such as AIDS. Caspase-associated recruitment domain 9 (CARD9) plays a critical role in the host defense against cryptococcal infection, suggesting the involvement of one or more C-type lectin receptors (CLRs). In the present study, we analyzed the role of macrophage-inducible C-type lectin (Mincle), one of the CLRs, in the host defense against C. deneoformans infection. Mincle expression in the lungs of wild-type (WT) mice was increased in the early stage of cryptococcal infection in a CARD9-dependent manner. In Mincle gene-disrupted (Mincle KO) mice, the clearance of this fungus, pathological findings, Th1/Th2 response, and antimicrobial peptide production in the infected lungs were nearly comparable to those in WT mice. However, the production of interleukin-22 (IL-22), tumor necrosis factor alpha (TNF-α), and IL-6 and the expression of AhR were significantly decreased in the lungs of Mincle KO mice compared to those of WT mice. In in vitro experiments, TNF-α production by bone marrow-derived dendritic cells was significantly decreased in Mincle KO mice. In addition, the disrupted lysates of C. deneoformans, but not those of whole yeast cells, activated Mincle-triggered signaling in an assay with a nuclear factor of activated T cells (NFAT)-green fluorescent protein (GFP) reporter cells expressing this receptor. These results suggest that Mincle may be involved in the production of Th22-related cytokines at the early stage of cryptococcal infection, although its role may be limited in the host defense against infection with C. deneoformans.

  19. Production of IL-17A at Innate Immune Phase Leads to Decreased Th1 Immune Response and Attenuated Host Defense against Infection with Cryptococcus deneoformans. International-journal Peer-reviewed

    Ko Sato, Hideki Yamamoto, Toshiki Nomura, Jun Kasamatsu, Tomomitsu Miyasaka, Daiki Tanno, Ikumi Matsumoto, Takafumi Kagesawa, Anna Miyahara, Tong Zong, Akiho Oniyama, Kotone Kawamura, Rin Yokoyama, Yuki Kitai, Shigenari Ishizuka, Emi Kanno, Hiromasa Tanno, Hiromi Suda, Masanobu Morita, Masayuki Yamamoto, Yoichiro Iwakura, Keiko Ishii, Kazuyoshi Kawakami

    Journal of immunology (Baltimore, Md. : 1950)　205　(3)　686-698　2020/06/19

    DOI： 10.4049/jimmunol.1901238 　

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    IL-17A is a proinflammatory cytokine produced by many types of innate immune cells and Th17 cells and is involved in the elimination of extracellularly growing microorganisms, yet the role of this cytokine in the host defense against intracellularly growing microorganisms is not well known. Cryptococcus deneoformans is an opportunistic intracellular growth fungal pathogen that frequently causes fatal meningoencephalitis in patients with impaired immune responses. In the current study, we analyzed the role of IL-17A in the host defense against C. deneoformans infection. IL-17A was quickly produced by γδT cells at an innate immune phase in infected lungs. In IL-17A gene-disrupted mice, clearance of this fungal pathogen and the host immune response mediated by Th1 cells were significantly accelerated in infected lungs compared with wild-type mice. Similarly, killing of this fungus and production of inducible NO synthase and TNF-α were significantly enhanced in IL-17A gene-disrupted mice. In addition, elimination of this fungal pathogen, Th1 response, and expression of IL-12Rβ2 and IFN-γ in NK and NKT cells were significantly suppressed by treatment with rIL-17A. The production of IL-12p40 and TNF-α from bone marrow-derived dendritic cells stimulated with C. deneoformans was significantly suppressed by rIL-17A. In addition, rIL-17A attenuated Th1 cell differentiation in splenocytes from transgenic mice highly expressing TCR for mannoprotein 98, a cryptococcal Ag, upon stimulation with recombinant mannoprotein 98. These data suggest that IL-17A may be involved in the negative regulation of the local host defense against C. deneoformans infection through suppression of the Th1 response.

  20. Novel Toll-Like Receptor 9 Agonist Derived from Cryptococcus neoformans Attenuates Allergic Inflammation Leading to Asthma Onset in Mice. International-journal Peer-reviewed

    Kaori Dobashi-Okuyama, Kazuyoshi Kawakami, Tomomitsu Miyasaka, Ko Sato, Keiko Ishii, Kaori Kawakami, Chiaki Masuda, Syugo Suzuki, Jun Kasamatsu, Hideki Yamamoto, Daiki Tanno, Emi Kanno, Hiromasa Tanno, Tasuku Kawano, Motoaki Takayanagi, Tomoko Takahashi, Isao Ohno

    International archives of allergy and immunology　181　(9)　651-664　2020

    Publisher: S. Karger AG

    DOI： 10.1159/000508535 　

    ISSN： 1018-2438

    eISSN： 1423-0097

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    INTRODUCTION: The enhanced type 2 helper (Th2) immune response is responsible for the pathogenesis of allergic asthma. To suppress the enhanced Th2 immune response, activation of the Th1 immune response has been an alternative strategy for anti-asthma therapy. In this context, effective Th1-inducing adjuvants that inhibit the development of allergic asthma but do not flare the side effects of the primary agent are required in clinical treatment and preventive medicine. OBJECTIVE: In this study, we aimed to determine the regulation of the Th2 type immune response in asthma by a novel immunostimulatory oligodeoxynucleotide (ODN) derived from Cryptococcus neoformans, termed ODN112, which contains a cytosine-guanine (CG) sequence but not canonical CpG motifs. METHODS: Using an ovalbumin-induced asthma mouse model, we assessed the effect of ODN112 on prototypical asthma-related features in the lung and on the Th1/Th2 profile in the lymph nodes and lung of mice treated with ODN112 during sensitization. RESULTS AND CONCLUSION: ODN112 treatment attenuated asthma features in mice. In the bronchial lymph nodes of the lungs and in the spleen, ODN112 increased interferon-γ production and attenuated Th2 recall responses. In dendritic cells (DCs) after allergen sensitization, ODN112 enhanced cluster of differentiation (CD) 40 and CD80 expression but did not alter CD86 expression. Interleukin-12p40 production from DCs was also increased in a Th2-polarizing condition. Our results suggest that ODN112 is a potential Th1-inducing adjuvant during Th2 cell differentiation in the sensitization phase.

  21. Defect of Interferon γ Leads to Impaired Wound Healing through Prolonged Neutrophilic Inflammatory Response and Enhanced MMP-2 Activation. International-journal Peer-reviewed

    Emi Kanno, Hiromasa Tanno, Airi Masaki, Ayako Sasaki, Noriko Sato, Maiko Goto, Mayu Shisai, Kenji Yamaguchi, Naoyuki Takagi, Miki Shoji, Yuki Kitai, Ko Sato, Jun Kasamatsu, Keiko Ishii, Tomomitsu Miyasaka, Kaori Kawakami, Yoshimichi Imai, Yoichiro Iwakura, Ryoko Maruyama, Masahiro Tachi, Kazuyoshi Kawakami

    International journal of molecular sciences　20　(22)　2019/11/12

    DOI： 10.3390/ijms20225657 　

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    Interferon (IFN)-γ is mainly secreted by CD4+ T helper 1 (Th1), natural killer (NK) and NKT cells after skin injury. Although IFN-γ is well known regarding its inhibitory effects on collagen synthesis by fibroblasts in vitro, information is limited regarding its role in wound healing in vivo. In the present study, we analyzed how the defect of IFN-γ affects wound healing. Full-thickness wounds were created on the backs of wild type (WT) C57BL/6 and IFN-γ-deficient (KO) mice. We analyzed the percent wound closure, wound breaking strength, accumulation of leukocytes, and expression levels of COL1A1, COL3A1, and matrix metalloproteinases (MMPs). IFN-γKO mice exhibited significant attenuation in wound closure on Day 10 and wound breaking strength on Day 14 after wound creation, characteristics that are associated with prolonged neutrophil accumulation. Expression levels of COL1A1 and COL3A1 mRNA were lower in IFN-γKO than in WT mice, whereas expression levels of MMP-2 (gelatinase) mRNA were significantly greater in IFN-γKO than in WT mice. Moreover, under neutropenic conditions created with anti-Gr-1 monoclonal antibodies, wound closure in IFN-γKO mice was recovered through low MMP-2 expression levels. These results suggest that IFN-γ may be involved in the proliferation and maturation stages of wound healing through the regulation of neutrophilic inflammatory responses.

  22. TNF Receptor-Associated Factor 5 Limits Function of Plasmacytoid Dendritic Cells by Controlling IFN Regulatory Factor 5 Expression. International-journal Peer-reviewed

    Kobayashi S, Sakurai T, So T, Shiota Y, Asao A, Phung HT, Tanaka R, Kawabe T, Maruyama T, Kanno E, Kawakami K, Owada Y, Ishii N

    Journal of immunology (Baltimore, Md. : 1950)　203　(6)　1447-1456　2019/09

    DOI： 10.4049/jimmunol.1900188 　

    ISSN： 0022-1767

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    The physiological functions of TNF receptor-associated factor 5 (TRAF5) in the skin inflammation and wound healing process are not well characterized. We found that Traf5-/- mice exhibited an accelerated skin wound healing as compared with wild-type counterparts. The augmented wound closure in Traf5-/- mice was associated with a massive accumulation of plasmacytoid dendritic cells (pDCs) into skin wounds and an enhanced expression of genes related to wound repair at skin sites. In accordance with this result, adoptive transfer of Traf5-/- pDCs, but not wild-type pDCs, into the injured skin area in wild-type recipient mice significantly promoted skin wound healing. The expression of skin-tropic chemokine receptor CXCR3 was significantly upregulated in Traf5-/- pDCs, and treatment with a CXCR3 inhibitor cancelled the promoted wound healing in Traf5-/- mice, suggesting a pivotal role of CXCR3 in pDC-dependent wound healing. Traf5-/- pDCs displayed significantly higher expression of IFN regulatory factor 5 (IRF5), which correlated with greater induction of proinflammatory cytokine genes and CXCR3 protein after stimulation with TLR ligands. Consistently, transduction of exogeneous TRAF5 in Traf5-/- pDCs normalized the levels of abnormally elevated proinflammatory molecules, including IRF5 and CXCR3. Furthermore, knockdown of IRF5 also rescued the abnormal phenotypes of Traf5-/- pDCs. Therefore, the higher expression and induction of IRF5 in Traf5-/- pDCs causes proinflammatory and skin-tropic characteristics of the pDCs, which may accelerate skin wound healing responses. Collectively, our results uncover a novel role of TRAF5 in skin wound healing that is mediated by IRF5-dependent function of pDCs.

  23. Dectin-2-mediated signaling leads to delayed skin wound healing through enhanced neutrophilic inflammatory response and neutrophil extracellular traps formation. Peer-reviewed

    Miura T, Kawakami K, Kanno E, Tanno H, Tada H, Sato N, Masaki A, Yokoyama R, Kawamura K, Kitai Y, Takagi N, Yamaguchi K, Yamaguchi N, Kyo Y, Ishii K, Imai Y, Saijo S, Iwakura Y, Tachi M

    The Journal of investigative dermatology　139　(3)　702-711　2018/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.jid.2018.10.015 　

    ISSN： 0022-202X

  24. 緑膿菌接種創の治癒過程と菌排除におけるNKT細胞欠損の影響

    丹野 寛大, 菅野 恵美, 佐藤 すずな, 山口 賢次, 石井 恵子, 丸山 良子, 川上 和義, 館 正弘

    日本褥瘡学会誌　20　(3)　294-294　2018/09

    Publisher: (一社)日本褥瘡学会

    ISSN： 1345-0417

  25. 【創傷治癒の最前線】急性創傷と慢性創傷の治癒過程

    丹野 寛大, 菅野 恵美

    WOC Nursing　6　(7)　5-12　2018/07

    Publisher: (株)医学出版

  26. クリプトコックス感染免疫応答におけるMincleの役割の解析

    佐藤 佑樹, 菅野 恵美, 石井 恵子, 川上 和義

    感染症学雑誌　92　(3)　482-482　2018/05

    Publisher: (一社)日本感染症学会

    ISSN： 0387-5911

  27. Attenuated accumulation of regulatory T cells and reduced production of interleukin 10 lead to the exacerbation of tissue injury in a mouse model of acute respiratory distress syndrome. International-journal Peer-reviewed

    Masahiko Toyama, Daisuke Kudo, Tetsuji Aoyagi, Tomomitsu Miyasaka, Keiko Ishii, Emi Kanno, Mitsuo Kaku, Shigeki Kushimoto, Kazuyoshi Kawakami

    Microbiology and immunology　62　(2)　111-123　2018/02

    DOI： 10.1111/1348-0421.12564 　

    ISSN： 0385-5600

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    Acute respiratory distress syndrome (ARDS) is a pathological condition that involves diffuse lung injury and severe hypoxemia caused by pulmonary and systemic diseases. We have established a mouse model of severe ARDS, developed by intratracheal injection of α-galactosylceramide (α-GalCer), an activator of natural killer T (NKT) cells, followed by LPS. In the present study, we used this model to investigate the regulatory mechanism in the early inflammatory response during acute lung injury. In α-GalCer/LPS-treated mice, the number of CD4+ CD25+ Foxp3+ regulatory T (Treg) cells and the expression of a Treg cell-tropic chemokine, secondary lymphoid-tissue chemokine (SLC), in the lungs was significantly lower than in mice treated with LPS alone. Giving recombinant (r)SLC increased the number of Treg cells in α-GalCer/LPS-treated mice. Treatment with anti-IFN-γ mAb enhanced the expression of SLC and the accumulation of Treg cells in the lungs of α-GalCer/LPS-treated mice, whereas giving recombinant (r)IFN-γ reduced the number of Treg cells in mice treated with LPS alone. IL-10 production was significantly lower in α-GalCer/LPS-treated mice than in mice treated with LPS alone. Giving rIL-10 prolonged survival and attenuated lung injury as a result of reduced production of inflammatory cytokines (such as IL-1β, IL-6, TNF-α, and IFN-γ) and chemokines (including MCP-1, RANTES, IP-10, Mig, MIP-2, and KC) in α-GalCer/LPS-treated mice. Treatment with anti-IFN-γ mAb enhanced IL-10 production in α-GalCer/LPS-treated mice. These results suggest that the attenuated accumulation of Treg cells may be involved in the development of severe ARDS through a reduction in the synthesis of IL-10.

  28. Experimental wound ischemia does not promote Pseudomonas aeruginosa biofilm formation. Peer-reviewed

    Emi Kanno, Hiromasa Tanno, Kenji Yamaguchi, Ayako Sasaki, Ryoko Maruyama, Masahiro Tachi

    Journal of Dermatology and Dermatologic Surgery　22　68-71　2018

  29. 創傷感染管理の最前線 Biofilm-based wound therapy バイオフィルムに対する治療・ケア 何をターゲットとするべきか?

    菅野 恵美, 川上 和義, 丹野 寛大, 山口 賢次, 館 正弘

    日本創傷治癒学会プログラム・抄録集　47回　75-75　2017/11

    Publisher: (一社)日本創傷治癒学会

  30. Contribution of CARD9-mediated signalling to wound healing in skin Peer-reviewed

    Emi Kanno, Kazuyoshi Kawakami, Hiromasa Tanno, Aiko Suzuki, Noriko Sato, Airi Masaki, Ayano Imamura, Naoyuki Takagi, Takayuki Miura, Hideki Yamamoto, Keiko Ishii, Hiromitsu Hara, Yoshimichi Imai, Ryoko Maruyama, Masahiro Tachi

    EXPERIMENTAL DERMATOLOGY　26　(11)　1097-1104　2017/11

    DOI： 10.1111/exd.13389 　

    ISSN： 0906-6705

    eISSN： 1600-0625

  31. Invariant NKT cells promote skin wound healing by preventing a prolonged neutrophilic inflammatory response. Peer-reviewed

    Tanno H, Kawakami K, Kanno E, Suzuki A, Takagi N, Yamamoto H, Ishii K, Imai Y, Maruyama R, Tachi M

    Wound Repair and Regeneration　25　(5)　805-815　2017/09

    DOI： 10.1111/wrr.12588 　

    ISSN： 1524-475X

  32. Invariant NKT cells promote skin wound healing by preventing a prolonged neutrophilic inflammatory response Peer-reviewed

    Hiromasa Tanno, Kazuyoshi Kawakami, Emi Kanno, Aiko Suzuki, Naoyuki Takagi, Hideki Yamamoto, Keiko Ishii, Yoshimichi Imai, Ryoko Maruyama, Masahiro Tachi

    Wound Repair and Regeneration　25　(5)　805-815　2017/09

    Publisher: Wiley

    DOI： 10.1111/wrr.12588 　

    ISSN： 1067-1927

  33. Development and evaluation of a quantitative assay detecting cytomegalovirus transcripts for preemptive therapy in allogeneic hematopoietic stem cell transplant recipients Peer-reviewed

    Keiko Ishi, Yasushi Onishi, Namiko Miyamura, Noriko Fukuhara, Kenichi Ishizawa, Mutsumi Nakanishi, Satoru Ohnaka, Tomomitsu Miyasaka, Emi Kanno, Kazuyoshi Kawakami, Hideo Harigae, Mitsuo Kaku

    JOURNAL OF MEDICAL VIROLOGY　89　(7)　1265-1273　2017/07

    DOI： 10.1002/jmv.24775 　

    ISSN： 0146-6615

    eISSN： 1096-9071

  34. Pedometer-determined physical activities of daily living can affect blood pressure regulation in healthy young adults after postural change Peer-reviewed

    Hongxia Yang, Yumika Kotaki, Junko Hoshi, Sarina Bao, Hiromasa Tanno, Emi Kanno, Ryoko Maruyama

    FASEB JOURNAL　31　2017/04

    ISSN： 0892-6638

    eISSN： 1530-6860

  35. Does Mozart's music have a relaxation effect? Peer-reviewed

    Junko Hoshi, Mako Sasaki, Misaki Sugawara, Hongxia Yang, Sarina Bao, Hiromasa Tannno, Emi Kanno, Ryoko Maruyama

    FASEB JOURNAL　31　2017/04

    ISSN： 0892-6638

    eISSN： 1530-6860

  36. クリプトコックス感染防御におけるMincleの役割

    佐藤 佑樹, 宮原 杏奈, 宗 童, 景澤 貴史, 菅野 恵美, 石井 恵子, 川上 和義

    感染症学雑誌　91　(臨増)　390-390　2017/03

    Publisher: (一社)日本感染症学会

    ISSN： 0387-5911

  37. IL-17A promotes neutrophilic inflammation and disturbs acute wound healing in skin Peer-reviewed

    Naoyuki Takagi, Kazuyoshi Kawakami, Emi Kanno, Hiromasa Tanno, Atsushi Takeda, Keiko Ishii, Yoshimichi Imai, Yoichiro Iwakura, Masahiro Tachi

    EXPERIMENTAL DERMATOLOGY　26　(2)　137-144　2017/02

    DOI： 10.1111/exd.13115 　

    ISSN： 0906-6705

    eISSN： 1600-0625

  38. Critical role of tumor necrosis factor-α in the early process of wound healing in skin. Peer-reviewed

    Masae Ritsu, Kazuyoshi Kawakami, Emi Kanno, Hiromasa Tanno, Keiko Ishii, Yoshimichi Imai, Ryoko Maruyama, Masahiro Tachi

    Journal of Dermatology and Dermatologic Surgery　21　(1)　14-19　2017

  39. マウス皮膚創傷治癒過程におけるMincle遺伝子欠損の影響

    佐藤 紀子, 菅野 恵美, 丹野 寛大, 正木 愛梨, 高木 尚之, 石井 恵子, 丸山 良子, 川上 和義, 館 正弘

    日本創傷治癒学会プログラム・抄録集　46回　189-189　2016/12

    Publisher: (一社)日本創傷治癒学会

  40. マウス皮膚創傷治癒過程におけるインターフェロン-γの影響に関する研究

    正木 愛梨, 菅野 恵美, 丹野 寛大, 佐藤 紀子, 石井 恵子, 丸山 良子, 川上 和義, 館 正弘

    日本創傷治癒学会プログラム・抄録集　46回　188-188　2016/12

    Publisher: (一社)日本創傷治癒学会

  41. NKT細胞の活性化が創傷治癒過程に与える影響 α-GalCer創部投与による検証

    丹野 寛大, 川上 和義, 菅野 恵美, 今村 彩乃, 佐藤 紀子, 正木 愛梨, 高木 尚之, 石井 恵子, 丸山 良子, 館 正弘

    日本創傷治癒学会プログラム・抄録集　46回　163-163　2016/12

    Publisher: (一社)日本創傷治癒学会

  42. マウス皮膚創傷治癒過程におけるCARD9を介したシグナル伝達の影響

    菅野 恵美, 川上 和義, 丹野 寛大, 鈴木 愛子, 佐藤 紀子, 正木 愛梨, 高木 尚之, 石井 恵子, 丸山 良子, 館 正弘

    日本創傷治癒学会プログラム・抄録集　46回　126-126　2016/12

    Publisher: (一社)日本創傷治癒学会

  43. Promotion of acute-phase skin wound healing by Pseudomonas aeruginosa C-4-HSL Peer-reviewed

    Emi Kanno, Kazuyoshi Kawakami, Shinichi Miyairi, Hiromasa Tanno, Aiko Suzuki, Rina Kamimatsuno, Naoyuki Takagi, Tomomitsu Miyasaka, Keiko Ishii, Naomasa Gotoh, Ryoko Maruyama, Masahiro Tachi

    INTERNATIONAL WOUND JOURNAL　13　(6)　1325-1335　2016/12

    DOI： 10.1111/iwj.12523 　

    ISSN： 1742-4801

    eISSN： 1742-481X

  44. Blunted Autonomic Responses and Low-Grade Inflammation in Mongolian Adults Born at Low Birth Weight Peer-reviewed

    Sarina Bao, Emi Kanno, Ryoko Maruyama

    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE　240　(2)　171-179　2016/10

    DOI： 10.1620/tjem.240.171 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  45. Reconsideration of iodine in wound irrigation: the effects on Pseudomonas aeruginosa biofilm formation Peer-reviewed

    E. Kanno, H. Tanno, A. Suzuki, R. Kamimatsuno, M. Tachi

    JOURNAL OF WOUND CARE　25　(6)　335-339　2016/06

    DOI： 10.12968/jowc.2016.25.6.335 　

    ISSN： 0969-0700

  46. Contribution of Invariant Natural Killer T Cells to Skin Wound Healing Peer-reviewed

    Hiromasa Tanno, Kazuyoshi Kawakami, Masae Ritsu, Emi Kanno, Aiko Suzuki, Rina Kamimatsuno, Naoyuki Takagi, Tomomitsu Miyasaka, Keiko Ishii, Yoshimichi Imai, Ryoko Maruyama, Masahiro Tachi

    AMERICAN JOURNAL OF PATHOLOGY　185　(12)　3248-3257　2015/12

    DOI： 10.1016/j.ajpath.2015.08.012 　

    ISSN： 0002-9440

    eISSN： 1525-2191

  47. 創傷治癒過程におけるNKT細胞活性化の影響

    丹野 寛大, 川上 和義, 菅野 恵美, 高木 尚之, 上松野 りな, 石井 恵子, 丸山 良子, 館 正弘

    日本創傷治癒学会プログラム・抄録集　45回　99-99　2015/11

    Publisher: (一社)日本創傷治癒学会

  48. 創傷治癒過程におけるI型インターフェロンの影響に関する研究

    上松野 りな, 菅野 恵美, 川上 和義, 丹野 寛大, 高木 尚之, 石井 恵子, 丸山 良子, 館 正弘

    日本創傷治癒学会プログラム・抄録集　45回　98-98　2015/11

    Publisher: (一社)日本創傷治癒学会

  49. 【褥瘡患者のQOL向上を目指したドレッシング材の活用】 創傷治癒と湿潤環境理論

    菅野 恵美, 館 正弘

    WOC Nursing　3　(10)　7-14　2015/10

    Publisher: (株)医学出版

  50. 【創傷と細菌感染を考える〜細菌感染を考慮した創傷管理〜】 総論 湿潤環境療法と感染症、クリティカルコロナイゼーションとバイオフィルム

    菅野 恵美, 館 正弘

    WOC Nursing　3　(9)　7-14　2015/09

    Publisher: (株)医学出版

  51. Cryptococcus neoformans Infection in Mice Lacking Type I Interferon Signaling Leads to Increased Fungal Clearance and IL-4-Dependent Mucin Production in the Lungs Peer-reviewed

    Ko Sato, Hideki Yamamoto, Toshiki Nomura, Ikumi Matsumoto, Tomomitsu Miyasaka, Tong Zong, Emi Kanno, Kazuko Uno, Keiko Ishii, Kazuyoshi Kawakami

    PLOS ONE　10　(9)　e0138291　2015/09

    DOI： 10.1371/journal.pone.0138291 　

    ISSN： 1932-6203

  52. 褥瘡に対する局所陰圧療法施行時における創傷治癒と栄養状態に関する考察

    須藤 洋子, 菅野 恵美, 高橋 悌子, 久慈 瑞希, 横田 佳那恵, 丸山 良子, 館 正弘

    日本褥瘡学会誌　17　(3)　411-411　2015/07

    Publisher: (一社)日本褥瘡学会

    ISSN： 1345-0417

  53. NKT細胞制御による新たな慢性皮膚潰瘍の治療法開発への基礎研究

    丹野 寛大, 川上 和義, 鈴木 愛子, 菅野 恵美, 高木 尚之, 上松野 りな, 石井 恵子, 丸山 良子, 館 正弘

    日本褥瘡学会誌　17　(3)　335-335　2015/07

    Publisher: (一社)日本褥瘡学会

    ISSN： 1345-0417

  54. 創傷治癒の基礎 IL-17Aによる好中球性炎症を介した創傷治癒遅延機構の解明

    高木 尚之, 川上 和義, 菅野 恵美, 丹野 寛大, 武田 睦, 上松野 りな, 石井 恵子, 館 正弘

    日本褥瘡学会誌　17　(3)　300-300　2015/07

    Publisher: (一社)日本褥瘡学会

    ISSN： 1345-0417

  55. Mongolian low birth weight young adults have higher risk of hypertension compared with Han Chinese and Japanese counterparts Peer-reviewed

    Sarina Bao, Haruka Sato, Konosuke Sasaki, Emi Kanno, Ryoko Maruyama

    FASEB JOURNAL　29　2015/04

    ISSN： 0892-6638

    eISSN： 1530-6860

  56. Dectin-2 deficiency promotes Th2 response and mucin production in the lungs after pulmonary infection with Cryptococcus neoformans. International-journal Peer-reviewed

    Yuri Nakamura, Ko Sato, Hideki Yamamoto, Kana Matsumura, Ikumi Matsumoto, Toshiki Nomura, Tomomitsu Miyasaka, Keiko Ishii, Emi Kanno, Masahiro Tachi, Sho Yamasaki, Shinobu Saijo, Yoichiro Iwakura, Kazuyoshi Kawakami

    Infection and immunity　83　(2)　671-81　2015/02

    DOI： 10.1128/IAI.02835-14 　

    ISSN： 1098-5522

    More details Close

    Dectin-2 is a C-type lectin receptor that recognizes high mannose polysaccharides. Cryptococcus neoformans, a yeast-form fungal pathogen, is rich in polysaccharides in its cell wall and capsule. In the present study, we analyzed the role of Dectin-2 in the host defense against C. neoformans infection. In Dectin-2 gene-disrupted (knockout) (Dectin-2KO) mice, the clearance of this fungus and the inflammatory response, as shown by histological analysis and accumulation of leukocytes in infected lungs, were comparable to those in wild-type (WT) mice. The production of type 2 helper T (Th2) cytokines in lungs was higher in Dectin-2KO mice than in WT mice after infection, whereas there was no difference in the levels of production of Th1, Th17, and proinflammatory cytokines between these mice. Mucin production was significantly increased in Dectin-2KO mice, and this increase was reversed by administration of anti-interleukin 4 (IL-4) monoclonal antibody (MAb). The levels of expression of β1-defensin, cathelicidin, surfactant protein A (Sp-A), and Sp-D in infected lungs were comparable between these mice. In in vitro experiments, IL-12p40 and tumor necrosis factor alpha (TNF-α) production and expression of CD86 and major histocompatibility complex (MHC) class II by bone marrow-derived dendritic cells and alveolar macrophages were completely abrogated in Dectin-2KO mice. Finally, the disrupted lysates of C. neoformans, but not of whole yeast cells, activated Dectin-2-triggered signaling in an assay with nuclear factor of activated T cells (NFAT)-green fluorescent protein (GFP) reporter cells expressing this receptor. These results suggest that Dectin-2 may oppose the Th2 response and IL-4-dependent mucin production in the lungs after infection with C. neoformans, and it may not be required for the production of Th1, Th17, and proinflammatory cytokines or for clearance of this fungal pathogen.

  57. Dectin-2 Deficiency Promotes Th2 Response and Mucin Production in the Lungs after Pulmonary Infection with Cryptococcus neoformans Peer-reviewed

    Yuri Nakamura, Ko Sato, Hideki Yamamoto, Kana Matsumura, Ikumi Matsumoto, Toshiki Nomura, Tomomitsu Miyasaka, Keiko Ishii, Emi Kanno, Masahiro Tachi, Sho Yamasaki, Shinobu Saijo, Yoichiro Iwakura, Kazuyoshi Kawakami

    INFECTION AND IMMUNITY　83　(2)　671-681　2015/02

    DOI： 10.1128/IAI.02835-14 　

    ISSN： 0019-9567

    eISSN： 1098-5522

  58. 創傷における細菌コントロールの意義. Invited Peer-reviewed

    菅野恵美

    東北大学保健学科紀要　24　(1)　15-18　2015

  59. 緑膿菌が創部アポトーシス誘導に与える影響. Invited Peer-reviewed

    菅野 恵美

    東北医誌　127　91-91　2015

  60. 創傷治癒における微生物負荷の意義 Invited Peer-reviewed

    菅野 恵美

    基礎科学をもとにしたCo-Medical研究会雑誌　3　(1)　16-21　2015

  61. 緑膿菌が創部アポトーシス誘導に与える影響

    菅野 恵美, 川上 和義, 丹野 寛大, 鈴木 愛子, 上松野 りな, 石井 恵子, 高木 尚之, 丸山 良子, 館 正弘

    日本創傷治癒学会プログラム・抄録集　44回　75-75　2014/12

    Publisher: (一社)日本創傷治癒学会

  62. 創傷治癒におけるIL-17の役割に関する研究

    高木 尚之, 川上 和義, 菅野 恵美, 丹野 寛大, 武田 睦, 鈴木 愛子, 上松野 りな, 石井 恵子, 館 正弘

    日本創傷治癒学会プログラム・抄録集　44回　58-58　2014/12

    Publisher: (一社)日本創傷治癒学会

  63. 創傷治癒過程における1型インターフェロンの関与

    上松野 りな, 菅野 恵美, 川上 和義, 丹野 寛大, 鈴木 愛子, 高木 尚之, 石井 恵子, 丸山 良子, 館 正弘

    日本創傷治癒学会プログラム・抄録集　44回　57-57　2014/12

    Publisher: (一社)日本創傷治癒学会

  64. マウス皮膚創傷治癒過程におけるCARD9遺伝子欠損の影響

    鈴木 愛子, 菅野 恵美, 川上 和義, 丹野 寛大, 上松野 りな, 高木 尚之, 石井 恵子, 丸山 良子, 館 正弘

    日本創傷治癒学会プログラム・抄録集　44回　57-57　2014/12

    Publisher: (一社)日本創傷治癒学会

  65. NKT細胞による好中球性炎症反応の制御を介した皮膚創傷治癒の促進

    丹野 寛大, 川上 和義, 菅野 恵美, 鈴木 愛子, 上松野 りな, 石井 恵子, 丸山 良子, 館 正弘

    日本創傷治癒学会プログラム・抄録集　44回　55-55　2014/12

    Publisher: (一社)日本創傷治癒学会

  66. 【適切なトリアージと処置に活かす足病変の診断とフットケアの実際】 下肢感染症の評価と栄養管理

    菅野 恵美, 館 正弘

    WOC Nursing　2　(11)　58-65　2014/11

    Publisher: (株)医学出版

  67. Defect of CARD9 leads to impaired accumulation of gamma interferon-producing memory phenotype T cells in lungs and increased susceptibility to pulmonary infection with Cryptococcus neoformans. International-journal Peer-reviewed

    Hideki Yamamoto, Yuri Nakamura, Ko Sato, Yurie Takahashi, Toshiki Nomura, Tomomitsu Miyasaka, Keiko Ishii, Hiromitsu Hara, Natsuo Yamamoto, Emi Kanno, Yoichiro Iwakura, Kazuyoshi Kawakami

    Infection and immunity　82　(4)　1606-15　2014/04

    DOI： 10.1128/IAI.01089-13 　

    ISSN： 1098-5522

    More details Close

    Caspase recruitment domain-containing protein 9 (CARD9) is an adaptor molecule signal that is critical for NF-κB activation and is triggered through C-type lectin receptors (CLRs), which are pattern recognition receptors that recognize carbohydrate structures. Previous studies have reported that Cryptococcus neoformans, a fungal pathogen that causes meningoencephalitis in AIDS patients, is recognized through some CLRs, such as mannose receptors or DC-SIGN. However, the role of CARD9 in the host defense against cryptococcal infection remains to be elucidated. In the present study, we analyzed the role of CARD9 in the host defense against pulmonary infection with C. neoformans. CARD9 gene-disrupted (knockout [KO]) mice were highly susceptible to this infection, as shown by the reduced fungal clearance in the infected lungs of CARD9 KO mice, compared to that in wild-type (WT) mice. Gamma interferon (IFN-γ) production was strongly reduced in CARD9 KO mice during the innate-immunity phase of infection. Reduced IFN-γ synthesis was due to impaired accumulation of NK and memory phenotype T cells, which are major sources of IFN-γ innate-immunity-phase production; a reduction in the accumulation of these cells was correlated with reduced CCL4, CCL5, CXCL9, and CXCL10 synthesis. However, differentiation of Th17 cells, but not of Th1 cells, was impaired at the adaptive-immunity phase in CARD9 KO mice compared to WT mice, although there was no significant difference in the infection susceptibility between interleukin 17A (IL-17A) KO and WT mice. These results suggest that CARD9 KO mice are susceptible to C. neoformans infection probably due to the reduced accumulation of IFN-γ-expressing NK and memory phenotype T cells at the early stage of infection.

  68. Effect of low birth weight on inflammation biomarkers and autonomic function in healthy young adults Peer-reviewed

    Sarina Bao, Konosuke Sasaki, Haruka Sato, Emi Kanno, Ryoko Maruyama

    FASEB JOURNAL　28　(1)　2014/04

    ISSN： 0892-6638

    eISSN： 1530-6860

  69. Hemodynamic responses to recumbent positions in healthy adults Peer-reviewed

    Konosuke Sasaki, Haruka Sato, Sarina Bao, Emi Kanno, Ryoko Maruyama

    FASEB JOURNAL　28　(1)　2014/04

    ISSN： 0892-6638

    eISSN： 1530-6860

  70. Impaired autonomic regulation in hyperglycemia and cardiovascular dynamics during autologous blood donation Peer-reviewed

    Miho Kamakura, Emi Kanno, Masako Takahashi, Ryoko Maruyama

    FASEB JOURNAL　28　(1)　2014/04

    ISSN： 0892-6638

    eISSN： 1530-6860

  71. Sex differences in heart rate variability and circulation after postural change Peer-reviewed

    Haruka Sato, Konosuke Sasaki, Sarina Bao, Emi Kanno, Ryoko Maruyama

    FASEB JOURNAL　28　(1)　2014/04

    ISSN： 0892-6638

    eISSN： 1530-6860

  72. 局所陰圧療法施行時における疼痛管理の重要性

    高橋 悌子, 菅野 恵美, 須藤 洋子, 丸山 良子, 館 正弘

    日本創傷・オストミー・失禁管理学会誌　18　(2)　219-219　2014/04

    Publisher: (一社)日本創傷・オストミー・失禁管理学会

    ISSN： 1884-233X

  73. Defect of CARD9 Leads to Impaired Accumulation of Gamma Interferon-Producing Memory Phenotype T Cells in Lungs and Increased Susceptibility to Pulmonary Infection with Cryptococcus neoformans Peer-reviewed

    Hideki Yamamoto, Yuri Nakamura, Ko Sato, Yurie Takahashi, Toshiki Nomura, Tomomitsu Miyasaka, Keiko Ishii, Hiromitsu Hara, Natsuo Yamamoto, Emi Kanno, Yoichiro Iwakura, Kazuyoshi Kawakami

    INFECTION AND IMMUNITY　82　(4)　1606-1615　2014/04

    DOI： 10.1128/IAI.01089-13 　

    ISSN： 0019-9567

    eISSN： 1098-5522

  74. Involvement of Gr-1 dull+ cells in the production of TNF-α and IL-17 and exacerbated systemic inflammatory response caused by lipopolysaccharide Peer-reviewed

    Tanno, D, Akahori, Y, Toyama, M, Sato, K, Kudo, D, Abe, Y, Miyasaka, T, Yamamoto, H, Ishii, K, Kanno, E, Maruyama, R, Kushimoto, S, Iwakura, Y, Kawakami, K

    Inflammation　37　186-195　2014/02

    DOI： 10.1007/s10753-013-9729-5 　

    ISSN： 1573-2576

  75. Involvement of Gr-1(dull+) Cells in the Production of TNF-alpha and IL-17 and Exacerbated Systemic Inflammatory Response Caused by Lipopolysaccharide Peer-reviewed

    Daiki Tanno, Yukiko Akahori, Masahiko Toyama, Ko Sato, Daisuke Kudo, Yuzuru Abe, Tomomitsu Miyasaka, Hideki Yamamoto, Keiko Ishii, Emi Kanno, Ryoko Maruyama, Shigeki Kushimoto, Yoichiro Iwakura, Kazuyoshi Kawakami

    INFLAMMATION　37　(1)　186-195　2014/02

    DOI： 10.1007/s10753-013-9729-5 　

    ISSN： 0360-3997

    eISSN： 1573-2576

  76. 【小外傷・熱傷・褥瘡 創傷に対応する「ワザ」と「知恵」】 褥瘡ケアを実践するための創の見方 褥瘡の部位・状態による創傷被覆材の選び方と使い分け

    菅野 恵美, 館 正弘

    薬局　64　(12)　3026-3032　2013/11

    Publisher: (株)南山堂

    ISSN： 0044-0035

  77. Involvement of high mobility group box 1 and the therapeutic effect of recombinant thrombomodulin in a mouse model of severe acute respiratory distress syndrome Peer-reviewed

    Kudo, D, Toyama, M, Aoyagi, T, Akahori, Y, Yamamoto, H, Ishii, K, Kanno, E, Maruyama, R, Kaku, M, Kushimoto, S, Kawakami K

    Clinical and Experimental Immunology　173　276-287　2013/08

    DOI： 10.1111/cei.12106 　

    ISSN： 1365-2249

  78. Low-dose Interferon-alpha Treatment Improves Survival and Inflammatory Responses in a Mouse Model of Fulminant Acute Respiratory Distress Syndrome Peer-reviewed

    Daisuke Kudo, Kazuko Uno, Tetsuji Aoyagi, Yukiko Akahori, Keiko Ishii, Emi Kanno, Ryoko Maruyama, Shigeki Kushimoto, Mitsuo Kaku, Kazuyoshi Kawakami

    INFLAMMATION　36　(4)　812-820　2013/08

    DOI： 10.1007/s10753-013-9607-1 　

    ISSN： 0360-3997

  79. Neutrophil-derived tumor necrosis factor-α contributes to acute wound healing promoted by N-(3-oxododecanoyl)-l-homoserine lactone from Pseudomonas aeruginosa Peer-reviewed

    Emi Kanno, Kazuyoshi Kawakami, Shinichi Miyairi, Hiromasa Tanno, Hirono Otomaru, Arina Hatanaka, Shiori Sato, Keiko Ishii, Denso Hayashi, Nobuhito Shibuya, Yoshimichi Imai, Naomasa Gotoh, Ryoko Maruyama, Masahiro Tachi

    Journal of Dermatological Science　70　(2)　130-138　2013/05

    DOI： 10.1016/j.jdermsci.2013.01.004 　

    ISSN： 0923-1811 1873-569X

    eISSN： 1873-569X

  80. Age dependency of autonomic response to recumbent positions Peer-reviewed

    Ryuhei Sato, Konosuke Sasaki, Mayu Haga, Haruka Sato, Yurie Kimura, Emi Kanno, Ryoko Maruyama

    FASEB JOURNAL　27　2013/04

    ISSN： 0892-6638

  81. ROLE OF INVARIANT NATURAL KILLER T CELL IN SKIN WOUND HEALING Invited Peer-reviewed

    H. Tanno, K. Kawakami, E. Kanno, K. Ishii, D. Hayashi, R. Maruyama, M. Tachi

    WOUND REPAIR AND REGENERATION　21　(1)　A9-A9　2013/01

    ISSN： 1067-1927

  82. Low-energy extracorporeal shock wave therapy enhances skin wound healing in diabetic mice: A critical role of endothelial nitric oxide synthase Peer-reviewed

    Denso Hayashi, Kazuyoshi Kawakami, Kenta Ito, Keiko Ishii, Hiromasa Tanno, Yoshimichi Imai, Emi Kanno, Ryoko Maruyama, Hiroaki Shimokawa, Masahiro Tachi

    WOUND REPAIR AND REGENERATION　20　(6)　887-895　2012/11

    DOI： 10.1111/j.1524-475X.2012.00851.x 　

    ISSN： 1067-1927

    eISSN： 1524-475X

  83. 皮膚創傷治癒過程におけるbFGF産生へのTNF-αの関与 Peer-reviewed

    菅野恵美, 丹野寛大, 館 正弘

    日本褥瘡学会誌　14　(2)　113-120　2012/06

    Publisher:

    ISSN： 1345-0417

  84. Skin wound healing and cytokine response promoted by a quorum-sensing molecule from Pseudomonas aeruginosa Peer-reviewed

    Emi Kanno, Kazuyoshi Kawakami, Shinichi Miyairi, Hiromasa Tanno, Keiko Ishii, Denso Hayashi, Ryoko Maruyama, Masahiro Tachi

    JOURNAL OF IMMUNOLOGY　188　2012/05

    ISSN： 0022-1767

  85. Toll-like receptor 9-dependent activation of bone marrow-derived dendritic cells by URA5 DNA from Cryptococcus neoformans. International-journal Peer-reviewed

    Misuzu Tanaka, Keiko Ishii, Yuri Nakamura, Akiko Miyazato, Atsuko Maki, Yuzuru Abe, Tomomitsu Miyasaka, Hideki Yamamoto, Yukiko Akahori, Misaki Fue, Yurie Takahashi, Emi Kanno, Ryoko Maruyama, Kazuyoshi Kawakami

    Infection and immunity　80　(2)　778-86　2012/02

    DOI： 10.1128/IAI.05570-11 　

    ISSN： 1098-5522

    More details Close

    Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis in immunocompromised patients. Recently, we reported that Toll-like receptor 9 (TLR9) is involved in host defense against C. neoformans: specifically, it detects the pathogen's DNA. In the present study, we aimed to elucidate the mechanisms underlying TLR9-mediated activation of innate immune responses by using the URA5 gene, which encodes a virulent component of this fungal pathogen. A PCR-amplified 345-bp URA5 gene fragment induced interleukin-12 p40 (IL-12p40) production by bone marrow-derived dendritic cells (BM-DCs) in a TLR9-dependent manner. Similar activity was detected in the 5' 129-bp DNA fragment of URA5 and in a synthesized oligodeoxynucleotide (ODN) with the same sequence. Shorter ODN fragments, which contained GTCGGT or GACGAT but had only 24 or 21 bases, induced IL-12p40 production and CD40 expression by BM-DCs, but this activity vanished when the CG sequence was replaced by GC or when a phosphorothioate modification was introduced. IL-12p40 production caused by active ODN was strikingly enhanced by treatment with DOTAP, a cationic lipid that increases the uptake of DNA by BM-DCs, though DOTAP failed to induce IL-12p40 production by inactive ODN and did not affect the activity of an ODN-containing canonical CpG motif. There was no apparent difference in intracellular trafficking between active and inactive ODNs. Finally, an extremely high dose of inactive ODN suppressed IL-12p40 production by BM-DCs that had been stimulated with active ODN. These results suggest that the C. neoformans URA5 gene activates BM-DCs through a TLR9-mediated signaling pathway, using a mechanism possibly independent of the canonical CpG motif.

  86. Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells by URA5 DNA from Cryptococcus neoformans Peer-reviewed

    Misuzu Tanaka, Keiko Ishii, Yuri Nakamura, Akiko Miyazato, Atsuko Maki, Yuzuru Abe, Tomomitsu Miyasaka, Hideki Yamamoto, Yukiko Akahori, Misaki Fue, Yurie Takahashi, Emi Kanno, Ryoko Maruyama, Kazuyoshi Kawakami

    INFECTION AND IMMUNITY　80　(2)　778-786　2012/02

    DOI： 10.1128/IAI.05570-11 　

    ISSN： 0019-9567

  87. Wound healing in skin promoted by inoculation with Pseudomonas aeruginosa PAO1: The critical role of tumor necrosis factor-alpha secreted from infiltrating neutrophils Peer-reviewed

    Emi Kanno, Kazuyoshi Kawakami, Masae Ritsu, Keiko Ishii, Hiromasa Tanno, Sohachi Toriyabe, Yoshimichi Imai, Ryoko Maruyama, Masahiro Tachi

    WOUND REPAIR AND REGENERATION　19　(5)　608-621　2011/09

    DOI： 10.1111/j.1524-475X.2011.00721.x 　

    ISSN： 1067-1927

  88. 緑膿菌PAO1株接種による創傷治癒の促進 創部に集積した好中球の役割

    菅野 恵美, 川上 和義, 丹野 寛大, 石井 恵子, 林 殿聡, 丸山 良子, 館 正弘

    形態・機能　10　(1)　39-39　2011/08

    Publisher: コ・メディカル形態機能学会

    ISSN： 1347-7145

    eISSN： 1884-6084

  89. Heart rate variability and cardiovascular dynamics in postmenopausal women and premenopausal female runners with menstrual disorder Peer-reviewed

    Mizuki Nagai, Kaori Shoji, Mayu Haga, Miho Kamakura, Haruka Chiba, Emi Kanno, Wurigumula Wurigumula, Takahiro Kawabata, Ryoko Maruyama

    FASEB JOURNAL　25　2011/04

    ISSN： 0892-6638

  90. Biofilm formation on rat skin wounds by Pseudomonas aeruginosa carrying the green fluorescent protein gene Peer-reviewed

    Emi Kanno, Souhachi Toriyabe, Lianbo Zhang, Yoshimichi Imai, Masahiro Tachi

    EXPERIMENTAL DERMATOLOGY　19　(2)　154-156　2010/02

    DOI： 10.1111/j.1600-0625.2009.00931.x 　

    ISSN： 0906-6705

  91. The effects of estrogen and aging on autonomic nerve system in healthy women Peer-reviewed

    Kaori Shoji, Konosuke Sasaki, Emi Kanno, Ikue Watanabe, Akiko Sekimata, Ryoko Maruyama

    JOURNAL OF PHYSIOLOGICAL SCIENCES　60　S186-S186　2010

    ISSN： 1880-6546

  92. ラット皮膚潰瘍創における緑膿菌バイオフィルムの微細構造 Peer-reviewed

    菅野恵美, 鳥谷部荘八, 館正弘

    形態・機能　6　(2)　111-118　2008/03

    DOI： 10.11172/keitaikinou2002.6.111 　

  93. Morphological study on the blood supply in cadaver sacral region - Comparison between average physique and emaciation Peer-reviewed

    Emi Kanno, Yumi Matsuda, Ryoko Maruyama, Hiroshi Watanabe

    FASEB JOURNAL　21　(6)　A967-A968　2007/04

    ISSN： 0892-6638

  94. 解剖実習遺体を用いた仙骨部の形態学的研究 褥瘡の初発部位と組織損傷の拡大方向に注目して Peer-reviewed

    菅野恵美, 渡辺皓

    日本褥瘡学会誌　5　(3)　515-524　2003/12

  95. 解剖実習遺体にみられた褥瘡に関する研究 Peer-reviewed

    菅野恵美, 宮本久美子, 渡辺皓

    北日本看護学会誌　5　(1)　9-15　2002/11

Show all ︎Show first 5

Misc. 72

  1. Differences in clinicopathological characteristics between lipohypertrophy and localized insulin-derived amyloidosis: A scoping review. International-journal

    Kanae Mukai, Hiromasa Tanno, Junko Sugama, Toshihiko Yanagita, Emi Kanno

    Chronic diseases and translational medicine　10　(1)　22-30　2024/03

    DOI： 10.1002/cdt3.98 　

    eISSN： 2095-882X

  2. Dectin-2シグナルを介した皮膚創傷治癒遅延へのNatural Killer T細胞欠損の影響

    丹野 寛大, 菅野 恵美, 佐々木 綾子, 黒坂 志歩, 及川 ゆかり, 石井 恵子, 川上 和義, 館 正弘

    日本創傷治癒学会プログラム・抄録集　50回　70-70　2020/11

    Publisher: (一社)日本創傷治癒学会

  3. 乳酸菌Enterococcus faecalis KH2株が皮膚創傷治癒に与える影響

    黒坂 志歩, 丹野 寛大, 菅野 恵美, 及川 ゆかり, 石井 恵子, 川上 和義, 館 正弘

    日本創傷治癒学会プログラム・抄録集　50回　75-75　2020/11

    Publisher: (一社)日本創傷治癒学会

  4. 褥瘡感染対策の新展開 褥瘡の感染・バイオフィルムに対する治療・ケア ターゲットの再考

    菅野 恵美, 川上 和義, 丹野 寛大, 山口 賢次, 館 正弘

    日本褥瘡学会誌　22　(3)　245-245　2020/08

    Publisher: (一社)日本褥瘡学会

    ISSN： 1345-0417

  5. 緑膿菌に学び、緑膿菌を操る 皮膚創傷治癒の免疫機構と緑膿菌感染とのクロストーク

    菅野 恵美, 川上 和義, 丹野 寛大, 山口 賢次, 館 正弘

    感染症学雑誌　94　(臨増)　154-155　2020/03

    Publisher: (一社)日本感染症学会

    ISSN： 0387-5911

    eISSN： 1884-569X

  6. 皮膚創傷治癒におけるDectin-1,2の役割とNETs形成との関連

    山口 賢次, 川上 和義, 菅野 恵美, 佐々木 綾子, 庄司 未樹, 三浦 孝行, 丹野 寛大, 高木 尚之, 今井 啓道, 館 正弘

    日本創傷治癒学会プログラム・抄録集　49回　108-108　2019/12

    Publisher: (一社)日本創傷治癒学会

  7. Natural killer T細胞が創部緑膿菌排除および治癒に与える影響

    佐々木 綾子, 丹野 寛大, 佐藤 すずな, 霜野 瑞葵, 石井 恵子, 丸山 良子, 館 正弘, 川上 和義, 菅野 恵美

    形態・機能　18　(1)　59-59　2019/08

    Publisher: コ・メディカル形態機能学会

    ISSN： 1347-7145

    eISSN： 1884-6084

  8. 乳酸菌Enterococcus faecalis局所投与による皮膚創傷治癒の促進

    丹野 寛大, 黒坂 志歩, 島谷 香波, 佐々木 綾子, 石井 恵子, 丸山 良子, 館 正弘, 川上 和義, 菅野 恵美

    形態・機能　18　(1)　60-60　2019/08

    Publisher: コ・メディカル形態機能学会

    ISSN： 1347-7145

    eISSN： 1884-6084

  9. 褥瘡の感染対策をもっと考えよう 褥瘡の感染予防における新たなアプローチ

    菅野 恵美

    日本褥瘡学会誌　21　(3)　272-272　2019/07

    Publisher: (一社)日本褥瘡学会

    ISSN： 1345-0417

  10. 乳酸菌Enterococcus faecalis KH株のもつ免疫賦活作用が創傷治癒に与える影響

    丹野 寛大, 菅野 恵美, 黒坂 志歩, 島谷 香波, 佐々木 綾子, 石井 恵子, 丸山 良子, 川上 和義, 館 正弘

    日本褥瘡学会誌　21　(3)　308-308　2019/07

    Publisher: (一社)日本褥瘡学会

    ISSN： 1345-0417

  11. 創傷治癒のプロセスとメカニズム(トランスレーショナルリサーチ) 皮膚創傷治癒におけるDectin-1、2シグナル活性化の影響の違い

    山口 賢次, 川上 和義, 菅野 恵美, 丹野 寛大, 佐々木 綾子, 三浦 孝行, 高木 尚之, 館 正弘

    日本創傷治癒学会プログラム・抄録集　48回　112-112　2018/11

    Publisher: (一社)日本創傷治癒学会

  12. Dectin-2シグナル活性化による創傷治癒遅延へのNatural Killer T細胞の関与

    佐々木 綾子, 菅野 恵美, 丹野 寛大, 石井 恵子, 丸山 良子, 川上 和義, 館 正弘

    日本創傷治癒学会プログラム・抄録集　48回　157-157　2018/11

    Publisher: (一社)日本創傷治癒学会

  13. 緑膿菌接種創の治癒過程におけるNatural Killer T細胞の役割

    丹野 寛大, 菅野 恵美, 佐藤 すずな, 霜野 瑞葵, 佐々木 綾子, 山口 賢次, 石井 恵子, 丸山 良子, 川上 和義, 館 正弘

    日本創傷治癒学会プログラム・抄録集　48回　171-171　2018/11

    Publisher: (一社)日本創傷治癒学会

  14. 【創傷治癒の最前線】 急性創傷と慢性創傷の治癒過程

    丹野 寛大, 菅野 恵美

    WOC Nursing　6　(7)　5-12　2018/07

    Publisher: (株)医学出版

  15. 創傷治癒と細菌感染 (特集 創部感染の予防とケア)

    丹野 寛大, 菅野 恵美

    看護技術　63　(9)　830-834　2017/08

    Publisher: メヂカルフレンド社

    ISSN： 0449-752X

  16. IL‐17Aは好中球性炎症反応を遷延させ急性創傷治癒の遅延に働く

    菅野恵美, 高木尚之, 丹野寛大, 石井恵子, 丸山良子, 岩倉洋一郎, 館正弘, 川上和義

    日本生体防御学会学術総会講演抄録集　28th　49　2017

  17. NKT細胞は好中球性炎症を制御し皮膚創傷治癒を促進する

    丹野寛大, 菅野恵美, 三浦孝之, 石井恵子, 中山俊憲, 谷口克, 丸山良子, 館正弘, 川上和義

    日本生体防御学会学術総会講演抄録集　28th　48　2017

  18. LACK OF NKT CELLS LEADS TO PERSISTED INFILTRATION OF NEUTROPHILS AND DELAYED WOUND HEALING PROCESS IN SKIN

    H. Tanno, K. Kawakami, E. Kanno, A. Suzuki, N. Takagi, R. Kamimatsuno, K. Ishii, R. Maruyama, M. Tachi

    WOUND REPAIR AND REGENERATION　24　(4)　A38-A38　2016/07

    ISSN： 1067-1927

    eISSN： 1524-475X

  19. EFFECT OF CARD9-DEFICIENCY ON THE WOUND HEALING PROCESS IN SKIN

    A. Suzuki, E. Kanno, K. Kawakami, H. Tanno, R. Kamimatsuno, N. Takagi, K. Ishii, R. Maruyama, M. Tachi

    WOUND REPAIR AND REGENERATION　24　(4)　A38-A38　2016/07

    ISSN： 1067-1927

    eISSN： 1524-475X

  20. INVOLVEMENT OF TYPE I INTERFERON ON THE WOUND HEALING IN SKIN

    R. Kamimatsuno, E. Kanno, K. Kawakami, H. Tanno, A. Suzuki, N. Takagi, K. Ishii, R. Maruyama, M. Tachi

    WOUND REPAIR AND REGENERATION　24　(4)　A39-A39　2016/07

    ISSN： 1067-1927

    eISSN： 1524-475X

  21. PSEUDOMONAS AERUGINOSA CONTRIBUTES TO APOPTOSIS INDUCTION ON THE WOUND SITES

    E. Kanno, K. Kawakami, H. Tanno, A. Suzuki, R. Kamimatsuno, K. Ishii, N. Takagi, R. Maruyama, M. Tachi

    WOUND REPAIR AND REGENERATION　24　(4)　A41-A41　2016/07

    ISSN： 1067-1927

    eISSN： 1524-475X

  22. マウス創傷治癒過程におけるCARD9遺伝子欠損の影響

    上松野りな, 鈴木愛子, 菅野恵美, 丹野寛大, 丹野寛大, 高木尚之, 石井恵子, 原博満, 丸山良子, 川上和義, 館正弘

    日本生体防御学会学術総会講演抄録集　26th　56　2015

  23. NKT細胞による好中球性炎症反応の制御を介した皮膚創傷治癒の促進

    丹野寛大, 丹野寛大, 菅野恵美, 鈴木愛子, 高木尚之, 上松野りな, 石井恵子, 岩倉洋一郎, 中山俊憲, 谷口克, 丸山良子, 川上和義, 館正弘

    日本生体防御学会学術総会講演抄録集　26th　60　2015

  24. 年齢と性差が体位変換時の自律神経活動および循環動態に与える影響

    佐藤 遥, 佐々木 康之輔, 包 薩日娜, 菅野 恵美, 丸山 良子

    日本看護技術学会学術集会講演抄録集　13回　127-127　2014/11

    Publisher: (一社)日本看護技術学会

  25. 局所陰圧療法施行時における疼痛管理の重要性

    高橋悌子, 菅野恵美, 須藤洋子, 丸山良子, 館正弘

    日本創傷・オストミー・失禁管理学会誌(Web)　18　(2)　P-1 (WEB ONLY)　2014/10/22

    ISSN： 1884-2321

  26. INVOLVEMENT OF INVARIANT NATURAL KILLER T CELLS IN THE ACUTE WOUND HEALING IN SKIN

    H. Tanno, K. Kawakami, E. Kanno, A. Suzuki, K. Ishii, R. Maruyama, M. Tachi

    WOUND REPAIR AND REGENERATION　22　(2)　A61-A61　2014/03

    ISSN： 1067-1927

    eISSN： 1524-475X

  27. PROMOTION OF ACUTE WOUND HEALING BY N-BUTANOYL-(L)-HOMOSERINE LACTONE FROM PSEUDOMONAS AERUGINOSA

    E. Kanno, H. Tanno, K. Kawakami, S. Miyairi, K. Ishii, R. Maruyama, M. Tachi

    WOUND REPAIR AND REGENERATION　22　(1)　A8-A8　2014/01

    ISSN： 1067-1927

    eISSN： 1524-475X

  28. 創傷治癒過程におけるサイトカイン,ケモカイン産生とNKT細胞の関与

    丹野寛大, 川上和義, 鈴木愛子, 菅野恵美, 高木尚之, 上松野りな, 石井恵子, 丸山良子, 館正弘

    日本生体防御学会学術総会講演抄録集　25th　21　2014

  29. 性差から見た体位と自律神経活動および循環動態

    佐藤 遥, 佐々木 康之輔, 芳賀 麻有, 菅野 恵美, 丸山 良子

    日本看護技術学会学術集会講演抄録集　12回　114-114　2013/09

    Publisher: (一社)日本看護技術学会

  30. 心臓血管疾患手術が術後の栄養状態に及ぼす影響

    佐々木 康之輔, 菅野 恵美, 丸山 良子

    日本看護技術学会学術集会講演抄録集　12回　126-126　2013/09

    Publisher: (一社)日本看護技術学会

  31. 食道がん患者を対象にPTPS発症群と非発症群の特徴的要因を明らかにする

    細萱順一, 菅野恵美, 丸山良子, 中村美鈴

    Pain Res　28　(2)　123-123　2013/07

    Publisher: 日本疼痛学会

    ISSN： 0915-8588

  32. 皮膚創傷治癒過程におけるCARD9遺伝子欠損の影響に関する研究

    鈴木愛子, 菅野恵美, 川上和義, 丹野寛大, 上松野りな, 石井恵子, 丸山良子, 館正弘

    日本創傷治癒学会プログラム・抄録集　43rd　123　2013

  33. 創傷治癒過程におけるサイトカイン,ケモカイン産生とNKT細胞の関与

    丹野寛大, 川上和義, 鈴木愛子, 中山史子, 菅野恵美, 高木尚之, 石井恵子, 古和田雪, 丸山良子, 館正弘

    日本創傷治癒学会プログラム・抄録集　43rd　126　2013

  34. 緑膿菌クオラムセンシング分子N‐butanoyl‐L‐homoserine lactoneが創傷治癒過程と創部炎症反応に与える影響

    鈴木愛子, 菅野恵美, 川上和義, 宮入伸一, 丹野寛大, 石井恵子, 丸山良子, 館正弘

    日本生体防御学会学術総会講演抄録集　24th　55　2013

  35. 皮膚の構造と機能.

    菅野恵美, 館 正弘

    理学療法　30　(4)　387-393　2013

  36. 褥瘡の部位・状態による創傷被覆材の選び方と使い分け.

    菅野恵美, 館 正弘

    薬局　64　(12)　106-112　2013

  37. 側臥位は自律神経活動にどのような影響を与えるのか?

    佐々木 康之輔, 芳賀 麻有, 永井 瑞希, 松井 憲子, 菅野 恵美, 丸山 良子

    日本看護技術学会学術集会講演抄録集　11回　123-123　2012/08

    Publisher: (一社)日本看護技術学会

  38. 睡眠時の姿勢はどのように決定されているのか?

    芳賀 麻有, 永井 瑞希, 佐々木 康之輔, 菅野 恵美, 丸山 良子

    日本睡眠学会定期学術集会プログラム・抄録集　37回　231-231　2012/06

    Publisher: (一社)日本睡眠学会

  39. マウス皮膚創傷治癒過程とNatural Killer T細胞:IFN‐γとIL‐4の関与について

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 石井恵子, 林殿聡, 丸山良子, 館正弘

    日本創傷治癒学会プログラム・抄録集　42nd　126　2012

  40. マウス皮膚創傷治癒過程におけるNatural Killer T細胞とIFN‐γ,IL‐4の役割

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 林殿聡, 石井恵子, 丸山良子, 館正弘

    日本生体防御学会学術総会講演抄録集　23rd　44　2012

  41. 緑膿菌クオラムセンシング分子N‐butanoyl‐L‐homoserine lactoneが創傷治癒過程に与える影響

    菅野恵美, 丹野寛大, 川上和義, 宮入伸一, 石井恵子, 丸山良子, 館正弘

    日本創傷治癒学会プログラム・抄録集　42nd　109　2012

  42. 女子長距離選手の無排卵性月経が自律神経系・心血管系に及ぼす影響

    永井瑞希, 芳賀麻有, 菅野恵美, 高橋和広, 丸山良子

    体力科学　60　(6)　653　2011/12/01

    ISSN： 0039-906X

  43. マットレスの違いによる自律神経活動の変化

    宍戸華奈子, 芳賀麻有, 永井瑞希, 菅野恵美, 丸山良子

    日本看護技術学会学術集会講演抄録集　10th　149　2011/10

  44. 緑膿菌クオラムセンシング機構が創傷治癒過程と創部炎症反応に与える影響

    菅野恵美, 川上和義, 乙丸礼乃, 石井恵子, 丹野寛大, 林殿総, 丸山良子, 館正弘

    日本じょくそう学会誌　13　(3)　452　2011/07/20

    ISSN： 1345-0417

  45. マウス皮膚創傷治癒におけるNatural Killer T細胞の役割

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 石井恵子, 林殿聡, 丸山良子, 館正弘

    日本じょくそう学会誌　13　(3)　454　2011/07/20

    ISSN： 1345-0417

  46. マウス皮膚創傷治癒過程におけるNatural Killer T細胞とIFN‐γの役割

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 林殿聡, 石井恵子, 丸山良子, 館正弘

    日本生体防御学会学術総会講演抄録集　22nd　28　2011

  47. 緑膿菌クオラムセンシング分子N‐3‐Oxododecanoyl‐homoserine lactoneが創傷治癒過程と創部炎症反応に与える影響

    菅野恵美, 川上和義, 乙丸礼乃, 石井恵子, 丹野寛大, 林殿聡, 丸山良子, 館正弘

    日本生体防御学会学術総会講演抄録集　22nd　49　2011

  48. マウス皮膚創傷治癒過程におけるNatural Killer T細胞の役割

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 石井恵子, 林殿聡, 丸山良子, 館正弘

    日本創傷治癒学会プログラム・抄録集　41st　69　2011

  49. 緑膿菌クオラムセンシング分子による創傷治癒の促進と創部炎症反応の誘導

    菅野恵美, 川上和義, 乙丸礼乃, 石井恵子, 丹野寛大, 林殿聡, 丸山良子, 館正弘

    日本創傷治癒学会プログラム・抄録集　41st　64　2011

  50. マットレスの安楽性の検討~マットレスの違いによる自律神経活動の変化から~

    芳賀麻有, 永井瑞希, 千葉春香, 菅野恵美, 渡邊生恵, 竹田和博, 土屋智久, 丸山良子

    日本看護技術学会学術集会講演抄録集　9th　164　2010/10

  51. マウス皮膚創傷治癒過程におけるTNF‐αとinvariant natural killer T細胞の役割

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 石井恵子, 林殿聡, 丸山良子, 館正弘

    日本生体防御学会学術総会講演抄録集　21st　36　2010

  52. 創傷治癒過程における緑膿菌接種の影響:集積した好中球によるTNF‐α産生に注目して

    菅野恵美, 川上和義, 石井恵子, 立雅恵, 丹野寛大, 鳥谷部荘八, 林殿聡, 丸山良子, 館正弘

    日本生体防御学会学術総会講演抄録集　21st　60　2010

  53. マウス皮膚創傷治癒過程におけるNatural Killer T細胞の役割とその作用におけるTNF‐αの関与

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 石井恵子, 林殿聡, 丸山良子, 館正弘

    日本創傷治癒学会プログラム・抄録集　40th　60　2010

  54. 皮膚創傷治癒過程におけるbFGF,VEGF産生へのTNF‐αの関与

    菅野恵美, 川上和義, 立雅恵, 鳥谷部荘八, 石井恵子, 丸山良子, 館正弘

    形態・機能　8　(1)　40　2009/08/27

    ISSN： 1347-7145

  55. 創傷治癒過程における緑膿菌接種の影響:好中球の働きに注目して

    菅野恵美, 川上和義, 立雅恵, 鳥谷部荘八, 石井恵子, 丸山良子, 館正弘

    日本じょくそう学会誌　11　(3)　332　2009/08/01

    ISSN： 1345-0417

  56. Contribution of quorum sensing to the development of biofilm and virulence of pseudomonas aeruginosa in rat wound infection

    Lianbo Zhang, Masahiro Tachi, Emi Kanno, Souhati Toriyabe, Masahiro Kosaka

    WOUND REPAIR AND REGENERATION　17　(1)　A3-A3　2009/01

    ISSN： 1067-1927

  57. The effect of 1% PVP-I solution for experimentally heavily colonized wounds

    Emi Kanno, Masahiro Tachi, Lianbo Zhang, Sohachi Toriyabe, Masahiro Kosaka

    WOUND REPAIR AND REGENERATION　17　(1)　A1-A1　2009/01

    ISSN： 1067-1927

  58. Making of a pseudomonas aeruginosa biofilm model in a rat skin ulcer

    Sohachi Toriyabe, Masahiro Tachi, Emi Kanno

    WOUND REPAIR AND REGENERATION　16　(1)　A7-A7　2008/01

    ISSN： 1067-1927

  59. Biofilm formation by pseudomonas aeruginosa on skin wounds

    Emi kanno

    PCEM(Touhoku Univ.Med.School)　27　21-25　2008

  60. Ultrastructural analysis of biofilm by Pseudomonas aeruginosa on rat skin wounds

    Emi Kanno, Sohachi Toriyabe, Masahiro Tachi

    形態・機能　6　(2)　111-118　2008

  61. 皮膚創傷治癒過程における好中球の集積とTNF-α産生 緑膿菌接種の影響

    立雅恵, 菅野恵美, 鳥谷部荘八, 石井恵子, 丸山良子, 川上和義, 館正弘

    補体シンポジウム講演集　45　107-107　2008

  62. ラット皮膚感染潰瘍におけるバイオフィルム形成と上皮化の関連性 Lasl、RhlI遺伝子欠損の影響に注目して

    菅野恵美, 張連波, 鳥谷部荘八, 館正弘

    日本褥瘡学会誌　10　(3)　449-449　2008

  63. 皮膚創傷治癒過程における好中球集積とTNF-α産生への緑膿菌接種の影響

    菅野恵美, 立雅恵, 鳥谷部荘八, 石井恵子, 丸山良子, 川上和義, 館正弘

    形態・機能　7　(1)　33-33　2008

  64. だいじょうぶ? 静脈注射 4. 静脈注射の部位, , 22(8), 25-26, 2007. (Nursing Today)

    菅野恵美

    Nursing Today　22　(8)　25-26　2007/07

  65. 褥瘡,慢性難治性皮膚潰瘍に関する組織学的研究(第44回保健学科学術研究会)

    菅野 恵美, 丸山 良子, 東北大学医学部保健学科, 東北大学医学部保健学科

    東北大学医学部保健学科紀要 = Bulletin of School of Health Sciences Tohoku University　16　(1)　37-38　2007/01/01

    Publisher: 東北大学医学部保健学科

    ISSN： 1348-8899

    More details Close

    報告Report

  66. ラット皮膚潰瘍創における緑膿菌バイオフィルムの微細構造

    菅野恵美, 鳥谷部荘八, 館正弘

    形態・機能　6　(1)　59-59　2007

  67. 皮膚潰瘍創における緑膿菌バイオフィルム

    菅野恵美, 鳥谷部荘八, 館正弘

    日本褥瘡学会誌　9　(4)　556-556　2007

  68. ラット皮膚潰瘍における緑膿菌バイオフィルム形成 LasI、Rh1I遺伝子欠損の影響に注目して

    張連波, 館正弘, 菅野恵美, 鳥谷部荘八, 小阪智大

    日本創傷治癒学会プログラム・抄録集　37回　80-80　2007

  69. 希釈ポビドンヨード溶液を用いた創洗浄の検討

    菅野恵美, 館正弘, 張連波, 鳥谷部荘八, 小阪智大

    日本創傷治癒学会プログラム・抄録集　37回　71-71　2007

  70. 皮膚潰瘍創における緑膿菌バイオフィルム形成. (PCEM (Tohoku Univ. Med. School))

    菅野恵美

    PCEM (Tohoku Univ. Med. School)　27　21-25　2007/01

  71. 皮膚潰瘍創における細菌バイオフィルム形成の検討 虚血の影響に注目して

    菅野恵美, 鳥谷部荘八, 館正弘

    形態・機能　5　(1)　35-35　2006

  72. ラット皮膚潰瘍における緑膿菌バイオフィルムモデルの作成 虚血とバイオフィルム形成について

    鳥谷部荘八, 館正弘, 菅野恵美

    日本創傷治癒学会プログラム・抄録集　36回　54-54　2006

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Books and Other Publications 23

  1. Wound hygiene (ウンドハイジーン) の概念.

    菅野恵美, 丹野寛大

    WOC Nursing. 医学出版　2021/07

  2. 創傷における感染防御機構, 影響を与える要因.

    丹野寛大, 菅野恵美

    WOC Nursing. 医学出版　2021/07

  3. 術後創傷に対する創傷処置.

    菅野恵美

    ナース専科, オンライン記事　2021/04

  4. Chapter11 感覚器系.

    渡辺皓, 菅野恵美

    ワンポイント問題集 解剖学. サイオ出版　2020/12

  5. NPWTi-dにおける感染制御の機序.

    菅野恵美

    第49回日本創傷治癒学会 記録集 スーツセミナー1 周期的洗浄併用陰圧閉鎖療法(NPWTi-d)における感染制御の機序と治療設定の実際. 3M・ケーシーアイ株式会社　2020/04

  6. 創傷ケアにおける感染対策‐バイオフィルムを可視化し、日常的なケアで制御する時代の到来‐.

    菅野恵美

    Hospital Communication. サラヤ株式会社　2020/03

  7. WOC Nursing 2018年7月 Vol.6No.7 特集:創傷治癒の最前線

    菅野 恵美

    医学出版　2018/07

    ISBN: 4287730569

  8. Visual Dermatology 研究者の目から見た皮膚科学の面白さ！－創傷治癒過程の華麗なるバトンパスに魅了されて－.

    秀潤社　2018

  9. フットケアと足病変治療ガイドブック.

    菅野恵美, 館正弘

    医学書院　2017

  10. フットケアと足病変治療ガイドブック. 第7章 足のアセスメントと検査. 1. プライマリケアで必要なアセスメント. I 潰瘍・感染徴候のアセスメント.

    菅野恵美, 館正弘

    医学書院　2017

  11. 看護技術 第1特集 創部感染の予防とケア. Part1 創傷治癒と細菌感染.

    丹野寛大, 菅野恵美

    メヂィカルフレンド社　2017

  12. 看護技術 第1特集 創部感染の予防とケア.

    KANNO Emi

    2017

  13. 新改訂 図解ワンポイント 解剖学. ChapterⅪ感覚器系.

    渡辺皓, ed, 渡辺皓, 菅野恵美

    サイオ出版　2016

  14. 術後ケアとドレーン管理のすべて. 第3章 創管理 創傷治癒過程.

    竹末芳生, 藤野智子, 菅野恵美, 館正弘

    照林社　2016

  15. 外用薬の特性に基づいた褥瘡外用療法のキホン. 3. 外用薬かドレッシング材かの判断のポイント.

    宮地良樹, 菅野恵美, 館正弘

    南山堂　2016

  16. 食生活からみた中国少数民族の出生体重と高血圧発症の関連.

    丸山良子, 菅野恵美, 包薩日娜, 佐藤遥, 佐々木康之輔

    公益財団法人アサヒビール学術振興財団　2015

  17. 総論-湿潤環境療法と感染症, クリティカルコロナイゼーションとバイオフィルム.

    館正弘 ed, 菅野恵美, 館正弘

    医学出版 WOC Nursing　2015

  18. 創傷治癒と湿潤環境理論

    貝谷敏子, 菅野恵美, 館正弘

    医学出版 WOC Nursing　2015

  19. 創傷のすべて

    市岡滋 e, 菅野恵美, 館

    克誠堂出版　2012/08

  20. ナースのためのアドバンスド創傷ケア

    真田弘美, 大浦紀彦, 溝上祐子, 市岡滋, ed, 菅野恵美, 館

    照林社　2012/08

  21. 創傷ケアの基礎知識と実践

    溝上祐子, 菅野恵美, 館

    メディカ出版　2011/05/10

  22. 創傷管理の必須知識

    渡辺皓, 菊地憲明, 館正弘 ed, 松田友美, 菅野恵美, 齋野幸子

    エルゼビア・ジャパン　2008/12

  23. 創傷管理の必須知識

    渡辺皓, 菊地憲明, 館正弘 ed, 齋野幸子, 菅野恵美, 松田友美

    エルゼビア・ジャパン　2008/12

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Presentations 97

  1. 創傷ケアに革新を！褥瘡の再発を防ぐナノ型乳酸菌を含有した創傷被覆材の創出. Invited

    菅野恵美

    官民による若手研究者発掘支援事業 社会実装目的型の医療機器創出支援プロジェクト, Tokyo Biodesign Medtech Innovation day 2025　2025/03/04

  2. 未来を支えるバイオ研究 Invited

    菅野恵美

    シンポジウム1 バイオ研究のすすめ. 第12回看護理工学会学術集会　2024/11/02

  3. 異分野融合・産学連携のきっかけとマインド. Invited

    菅野恵美

    第23回JANSセミナー, 日本看護科学学会　2024/03/18

  4. 褥瘡の再発を防ぐナノ型乳酸菌を含有した創傷被覆材の創出.

    菅野恵美

    官民による若手研究者発掘支援事業 社会実装目的型の医療機器創出支援プロジェクト, Tokyo Biodesign Medtech Innovation day 2024　2024/03/07

  5. Development of wound dressing for preventing severe pressure ulcers using nano-type lactic acid bacteria Invited

    菅野恵美

    Medtech Academy Pitch Day 2023/バイオデザイン学会　2023/03/03

  6. 便の付着から感染, 褥瘡重症化に至るメカニズムを考える. Invited

    菅野恵美

    第27回日本褥瘡学会学術集会, シンポジウム8 排便コントロールから考える褥瘡感染・重症化予防　2025/08/30

  7. 基礎研究からみえてきた！透明な創傷被覆材ATKPADの活用法 Invited

    菅野恵美

    第68回日本形成外科学会総会・学術集会 ランチョンセミナー　2025/04/18

  8. 肌の修復におけるβグルカンの効果 Invited

    菅野恵美

    ランコム 「ジェニフィック アルティメ セラム」製品発表会　2024/07/23

  9. ATKパッドの有効性を最大限に発揮するためには？～基礎研究からの考察～. Invited

    菅野恵美

    第33回日本創傷・オストミー・失禁管理学会学術集会 コーヒーブレイクセミナー　2024/05/25

  10. 褥瘡・創傷の創部の観察および処置方法について Invited

    菅野恵美

    医療法人ひろせ会広瀬病院 褥瘡予防対策委員会セミナー　2023/09/15

  11. 褥瘡の再発を防ぐナノ型乳酸菌を含有した創傷被覆材の創出. Invited

    菅野恵美

    官民による若手研究者発掘支援事業 社会実装目的型の医療機器創出支援プロジェクト 令和5年度 第7回メドテックマッチング　2023/08/29

  12. Current treatment and potential therapeutic targets of biofilms in chronic nonhealing wounds. Invited

    菅野恵美

    シンポジウム2, 第99回日本生理学会　2022/03/16

  13. Contribution of iNKT cells to the clearance of Pseudomonas aeriginosa from skin wounds.

    第47回日本免疫学会学術集会　2018/12/12

  14. Effect of interferon-γ deficiency on skin wound healing processes.

    Ayako SASAKI, Emi KANNO, Hiromasa TANNO, Keiko Ishii, Yoichiro IWAKURA, Kazuyoshi KAWAKAMI

    第47回日本免疫学会学術集会　2018/12/11

  15. Dectin-2-mediated signaling leads to delayed skin wound healing through enhanced neutrophilic inflammatory response and NETosis.

    第47回日本免疫学会学術集会 (Workshop-19)　2018/12/11

  16. 皮膚創傷治癒におけるDectin-1、2シグナル活性化の影響の違い.

    山口賢次, 川上和義, 菅野恵美, 丹野寛大, 佐々木綾子, 三浦考行, 高木尚之, 館正弘

    第48回日本創傷治癒学会　2018/11/30

  17. 緑膿菌接種創の治癒過程におけるNatural Killer T細胞の役割.

    丹野寛大, 菅野恵美, 佐藤すずな, 霜野瑞葵, 佐々木綾子, 山口賢次, 石井恵子, 丸山良子, 川上和義, 館正弘

    第48回日本創傷治癒学会　2018/11/30

  18. Dectin-2シグナル活性化による創傷治癒遅延へのNatural Killer T細胞の関与.

    佐々木綾子, 菅野恵美, 丹野寛大, 石井恵子, 丸山良子, 川上和義, 館正弘

    第48回日本創傷治癒学会　2018/11/29

  19. 緑膿菌接種創の治癒過程と菌排除におけるNKT細胞欠損の影響.

    丹野寛大, 菅野恵美, 佐藤すずな, 山口賢次, 石井恵子, 丸山良子, 川上和義, 館正弘

    第20回日本褥瘡学会学術集会　2018/09/28

  20. Does Mozart's and Bach's music have a relaxation effect?

    Junko Hoshi, Hongxia Yang, Xinru Sun, Hiromasa Tanno, Emi Kanno, Ryoko Maruyama

    第95回日本生理学会大会　2018/03/29

  21. 頭頸部放射線治療中の放射線皮膚炎に対してソフトシリコンタイプの創傷被覆材によるケアが奏功した事例

    門脇美佳, 菅野恵美, 小川武則, 後藤えり子

    第32回日本がん看護学会　2018/02/03

  22. Effect of Mincle deficiency on the wound healing process in skin.

    SATO Noriko, KANNO Emi, TANNO Hiromasa, MASAKI Airi, MIURA Takayuki, ISHII Keiko, YAMASAKI Sho, KAWAKAMI Kazuyoshi

    第46回日本免疫学会学術集会　2017/12/14

  23. Critical role of interferon (IFN)-γ in the skin wound healing.

    MASAKI Airi, KANNO Emi, TANNO Hiromasa, SATO Noriko, MIURA Takayuki, ISHII Keiko, IWAKURA Yoichiro, KAWAKAMI Kazuyoshi

    第46回日本免疫学会学術集会　2017/12/14

  24. Effect of Mincle-deficiency on the host immune response against Cryptococcus neoformans infection.

    Yuki SATO, Emi KANNO, Keiko Ishii, Sho YAMASAKI, Kazuyoshi KAWAKAMI

    第46回日本免疫学会学術集会　2017/12/12

  25. Effect of Dectin-2-mediated signaling on skin wound healing and NETosis.

    MIURA Takayuki, KANNO EMI, TANNO Hiromasa, SATO Noriko, MASAKI Airi, ISHII Keiko, SAIJO Shinobu, IWAKURA Yoichiro, KAWAKAMI Kazuyoshi

    第46回日本免疫学会学術集会　2017/12/12

  26. Cタイプレクチン受容体を介した皮膚炎症反応の制御.

    菅野恵美

    第1回東北医真菌研究会　2017/12/08

  27. 皮膚創傷におけるDectin-2を介した治癒過程およびNETosisの制御.

    三浦考行, 川上和義, 菅野恵美, 丹野寛大, 多田浩之, 佐藤紀子, 正木愛梨, 高木尚之, 山口賢次, 石井恵子, 館正弘

    第47回日本創傷治癒学会　2017/11/28

  28. バイオフィルムに対する治療・ケア－何をターゲットとするべきか？. Invited

    菅野恵美, 川上和義, 丹野寛大, 山口賢次, 館正弘

    第47回日本創傷治癒学会 シンポジウム4 創傷感染管理の最前線: Biofilm-based wound therapy　2017/11/28

  29. マウス皮膚創傷治癒過程におけるMincle遺伝子欠損の影響.

    佐藤紀子, 菅野恵美, 丹野寛大, 正木愛梨, 高木尚之, 石井恵子, 丸山良子, 川上和義, 館正弘

    第47回日本創傷治癒学会　2017/11/27

  30. 創傷治癒過程におけるインターフェロン-γ欠損の影響.

    正木愛梨, 菅野恵美, 丹野寛大, 佐藤紀子, 三浦考行, 山口賢次, 石井恵子, 川上和義, 館正弘

    第47回日本創傷治癒学会　2017/11/27

  31. 皮膚創傷治癒過程におけるNKT細胞の役割－好中球性炎症に注目して－.

    丹野寛大, 菅野恵美, 佐藤紀子, 正木愛梨, 三浦考行, 山口賢次, 石井恵子, 丸山良子, 川上和義, 館正弘

    第47回日本創傷治癒学会　2017/11/27

  32. 頭頸部放射線治療中の皮膚炎に対して創傷被覆材によるケアが奏功した事例.

    門脇美佳, 菅野恵美, 小川武則, 後藤えり子

    第30回日本放射線腫瘍学会　2017/11/17

  33. クリプトコックス感染免疫応答におけるMincleの役割の解析.

    佐藤佑樹, 菅野恵美, 石井恵子, 川上和義

    第66回日本感染症学会 東日本地方会学術集会・第64回日本化学療法学会 東日本支部総会 合同学会　2017/11/01

  34. NKT細胞は好中球性炎症を制御し皮膚創傷治癒を促進する.

    丹野寛大, 菅野恵美, 三浦孝行, 石井恵子, 中山俊憲, 谷口克, 丸山良子, 館正弘, 川上和義

    第28回日本生体防御学会学術総会　2017/06/30

  35. IL-17Aは好中球性炎症反応を遷延させ急性創傷治癒の遅延に働く.

    菅野恵美, 高木尚之, 丹野寛大, 石井恵子, 丸山良子, 岩倉洋一郎, 館正弘, 川上和義

    第28回日本生体防御学会学術総会　2017/06/30

  36. 看護師からみた褥瘡治療の現状～創状態に応じたドレッシング材の選定と求める機能性～．

    菅野恵美

    技術情報協会セミナーNo. 704116 ドレッシング材に求められる機能性と選択基準　2017/04/17

  37. マウス圧迫創におけるHMGB1陽性細胞の経時的変化

    松田 友美, 石田 陽子, 菅野 恵美

    第43回日本創傷治癒学会　2013/11/14

  38. 創傷治癒におけるIL-17欠損の影響に関する研究

    高木 尚之, 川上 和義, 丹野 寛大, 菅野 恵美, 石井 恵子, 館 正弘

    第43回日本創傷治癒学会　2013/11/14

  39. 創傷治癒におけるサイトカイン, ケモカイン産生とNKT細胞の関与

    丹野 寛大, 川上 和義, 鈴木 愛子, 中山 史子, 菅野 恵美, 高木 尚之, 石井 恵子, 古和田 雪, 丸山 良子, 館 正弘

    第43回日本創傷治癒学会　2013/11/14

  40. 皮膚創傷治癒過程におけるCARD9遺伝子欠損の影響に関する研究

    鈴木 愛子, 菅野 恵美, 川上 和義, 丹野 寛大, 上松野 りな, 石井 恵子, 丸山 良子, 館 正弘

    第43回日本創傷治癒学会　2013/11/14

  41. 心臓血管疾患手術が術後の栄養状態に及ぼす影響

    佐々木康之輔, 菅野恵美, 丸山良子

    第12回日本看護技術学会学術集会　2013/09/14

  42. 性差から見た体位と自律神経活動および循環動態

    佐藤 遥, 佐々木 康之輔, 芳賀 麻有, 菅野 恵美, 丸山 良子

    第12回日本看護技術学会学術集会　2013/09/14

  43. Reduced heart rate in left lateral decubitus position in healthy adults International-presentation

    Konosuke Sasaki, Mayu Haga, Haruka Sato, Yurie Kimura, Emi Kanno, Ryoko Maruyama

    The 37th World Congress of the International Union of Physiological Sciences　2013/07/21

  44. Higher risk of hypertension and autonomic nervous system imbalance in Mongolian young adults with low birth weight compared to Japanese counterparts. International-presentation

    Sarina Bao, Wurigumura, Eriko Takahashi, Manami Dendo, Konosuke Sasaki, Emi Kanno, Ryoko Maruyama

    The 37th World Congress of the International Union of Physiological Sciences　2013/07/21

  45. 食道がん患者を対象にPTPS発症群と非発症群の特徴的要因を明らかにする

    細萱順一, 菅野恵美, 丸山良子

    第35回日本疼痛学会学術集会　2013/07/12

  46. 緑膿菌クオラムセンシング分子N-butanoyl-L-homoserine lactoneが創傷治癒と創部炎症反応に与える影響

    鈴木愛子, 菅野恵美, 川上和義, 宮入伸一, 丹野寛大, 石井恵子, 丸山良子, 館正弘

    第24回日本生体防御学会　2013/07/10

  47. Predictive Factors For Post-Thoracotomy Pain Syndrome International-presentation

    Junichi Hosogaya, Emi Kanno, Ryoko Maruyama

    American Thoracic Society　2013/05/17

  48. Age dependency of autonomic response to recumbent positions International-presentation

    Ryuhei Sato, Konosuke Sasaki, Mayu Haga, Haruka Sato, Yurie Kimura, Emi Kanno, Ryoko Maruyama

    FASEB　2013/04/20

  49. Involvement of invariant Natural Killer T cells in the acute wound healing in skin.

    TANNO Hiromasa, KANNO Emi, ISHII Keiko, NAKAYAMA Toshinori, TANIGUCHI Masaru, KAWAKAMI Kazuyoshi

    第41回日本免疫学会学術集会　2012/12/05

  50. N-(3-oxododecanoyl)-L-homoserine lactone, a quorum-sensing molecule from Pseudomonas aeruginosa, promotes acute wound healing.

    KANNO Emi, TANNO Hiromasa, ISHII Keiko, KAWAKAMI Kazuyoshi

    第41回日本免疫学会学術集会　2012/12/05

  51. 緑膿菌クオラムセンシング分子N-butanoyl-L-homoserine lactoneが創傷治癒過程に与える影響

    菅野恵美, 丹野寛大, 川上和義, 宮入伸一, 石井恵子, 丸山良子, 館正弘

    第42回日本創傷治癒学会　2012/12/02

  52. マウス皮膚創傷治癒過程とNatural Killer T細胞: IFN-γとIL-4の関与について.

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 石井恵子, 林殿聡, 丸山良子, 館正弘

    第42回日本創傷治癒学会　2012/12/02

  53. 側臥位は自律神経活動にどのような影響を与えるのか？

    佐々木康之輔, 芳賀麻有, 永井瑞希, 松井憲子, 菅野恵美, 丸山良子

    第11回日本看護技術学会学術集会　2012/09/16

  54. Skin wound healing promoted by a quorum-sensing moleclue, N-(3-Oxododecanoyl)-L-homoserine lactone from Pseudomonas aeruginosa International-presentation

    Emi Kanno, Kazuyoshi Kawakami, Shinichi Miyairi, Hiromasa Tanno, Keiko Ishii, Denso Hayashi, Ryoko Maruyama, Masahiro Tachi

    4th Congress of the World Union of Wound Healing Societies　2012/09/02

  55. Role of invariant natural killer T cells in the skin wound healing International-presentation

    Hiromasa Tanno, Kazuyoshi Kawakami, Emi Kanno, Denso Hayashi, Keiko Ishii, Ryoko Maruyama, Masahiro Tachi

    4th Congress of the World Union of Wound Healing Societies　2012/09/02

  56. マウス皮膚創傷治癒過程におけるNatural Killer T細胞とIFNγ, IL-4の役割

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 林殿聡, 石井恵子, 丸山良子, 館正弘

    第23回日本生体防御学会　2012/07/09

  57. 睡眠時の姿勢はどのように決定されているのか？

    芳賀麻有, 永井瑞希, 佐々木康之輔, 菅野恵美, 丸山良子

    第37回日本睡眠学会学術集会　2012/06/28

  58. Skin wound healing and cytokine response promoted by a quorum-sensing moleclue from Pseudomonas aeruginosa International-presentation

    Emi Kanno, Kazuyoshi Kawakami, Shinichi Miyairi, Hiromasa Tanno, Keiko Ishii, Denso Hayashi, Ryoko Maruyama, Masahiro Tachi

    IMMUNOLOGY　2012/05/04

  59. 緑膿菌クオラムセンシング分子による創傷治癒の促進と創部炎症反応の誘導

    菅野恵美, 川上和義, 乙丸礼乃, 石井恵子, 丹野寛大, 林殿聡, 丸山良子, 館正弘

    第41回日本創傷治癒学会　2011/12/05

  60. マウス皮膚創傷治癒過程におけるNatural Killer T細胞の役割と

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 石井恵子, 林殿聡, 丸山良子, 館正弘

    第41回日本創傷治癒学会　2011/12/05

  61. マットレスの違いによる自律神経活動の変化

    宍戸華奈子, 芳賀麻有, 永井瑞希, 菅野恵美, 丸山良子

    日本看護技術学会第10回学術集会　2011/10/29

  62. 緑膿菌PAO1株接種による創傷治癒の促進：創部に集積した好中球の役割

    菅野恵美, 川上和義, 丹野寛大, 石井恵子, 林殿総, 丸山良子, 館正弘

    コメディカル形態機能学会 第10回学術集会　2011/09/17

  63. 緑膿菌クオラムセンシング機構が創傷治癒過程と創部炎症反応に与える影響

    菅野恵美, 川上和義, 乙丸礼乃, 石井恵子, 丹野寛大, 林殿聡, 丸山良子, 館正弘

    第13回日本褥瘡学会学術集会　2011/08/26

  64. マウス皮膚創傷治癒過程におけるNatural Killer T細胞との役割

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 石井恵子, 林殿聡, 丸山良子, 館正弘

    第13回日本褥瘡学会学術集会　2011/08/26

  65. マウス皮膚創傷治癒過程におけるNatural Killer T細胞とIFN-γの役割

    丹野寛大, 川上和義, 立雅恵, 菅野恵美, 林殿聡, 石井恵子, 丸山良子, 館正弘

    第22回日本生体防御学会学術集会　2011/06/29

  66. 緑膿菌クオラムセンシング分子N-3-Oxododecanoyl-homoserine lactoneが創傷治癒過程と創部炎症反応に与える影響

    菅野恵美, 川上和義, 乙丸礼乃, 石井恵子, 丹野寛大, 林殿聡, 丸山良子, 館正弘

    第22回日本生体防御学会学術集会　2011/06/29

  67. マウス皮膚創傷治癒過程におけるNatural Killer T細胞の役割とその作用におけるTNF-αの関与

    丹野 寛大, 川上 和義, 立 雅恵, 菅野 恵美, 石井 恵子, 林 殿聡, 丸山 良子, 館 正弘

    第40回日本創傷治癒学会　2010/12/02

  68. マットレスの安楽性の検討〜マットレスの違いによる自律神経活動の変化から〜

    芳賀麻有, 永井瑞希, 千葉春香, 菅野恵美, 渡邊生恵, 竹田和博, 土屋智久, 丸山良子

    日本看護技術学会第9回学術集会　2010/10/23

  69. Promoted wound healing in the skin by inoculation of Pseudomonas aeruginosa : role of TNF-α secreted from infiltrating neutrophils International-presentation

    Emi Kanno, Kazuyoshi Kawakami, Masae Ritsu, Keiko Ishii, Sohachi Toriyabe, Ryoko Maruyama, Masahiro Tachi

    14th International Congress of Immunology　2010/08/22

  70. Contribution of tumor necrosis factor-α and invariant natural killer T cell to skin wound healing in mice International-presentation

    Hiromasa Tanno, Kazuyoshi Kawakami, Masae Ritsu, Emi Kanno, Keiko Ishii, Sohachi Toriyabe, Ryoko Maruyama, Masahiro Tachi

    14th International Congress of Immunology　2010/08/22

  71. 創傷治癒過程における緑膿菌接種の影響：集積した好中球によるTNF-α産生に注目して

    菅野恵美, 川上和義, 石井恵子, 立 雅恵, 丹野寛大, 鳥谷部荘八, 林 殿聡, 丸山良子, 館 正弘

    第21回日本生体防御学会　2010/07/22

  72. マウス皮膚創傷治癒過程におけるTNF-αとinvariant natural killer T 細胞の役割

    丹野寛大, 川上和義, 菅野恵美, 立 雅恵, 石井恵子, 林 殿聡, 丸山良子, 館 正弘

    第21回日本生体防御学会　2010/07/22

  73. The effect of estrogen and aging on autonomic nerve system in

    Kaori Shoji, Konosuke Sasaki, Emi Kanno, Ikue Watanabe, Akiko Sekimata, Ryoko Maruyama

    第87回日本生理学会大会　2010/05/19

  74. 皮膚創傷治癒過程におけるbFGF、VEGF産生へのTNF-αの関与

    菅野恵美, 川上和義, 立 雅恵, 鳥谷部荘八, 石井恵子, 丸山良子, 館 正弘

    コ・メディカル形態機能学会 第8回学術集会　2009/09/12

  75. 創傷治癒過程における緑膿菌接種の影響：好中球の働きに注目して

    菅野恵美, 川上和義, 立 雅恵, 鳥谷部荘八, 石井恵子, 丸山良子, 館 正弘

    第11回日本褥瘡学会学術集会　2009/09/04

  76. Accumulation of neutrophils and TNF-α synthesis during wound healing process in the skin: effect of inoculation of Pseudomonas aeruginosa International-presentation

    Emi Kanno, Kazuyoshi Kawakami, Masae Ritsu, Sohachi Toriyabe, Keiko Ishii, Ryoko Maruyama, Masahiro Tachi

    EWMA 2009　2009/05/20

  77. 皮膚創傷治癒過程における好中球集積とTNF-α産生への緑膿菌接種の影響

    菅野恵美, 立 雅恵, 鳥谷部荘八, 石井恵子, 丸山良子, 川上和義, 館 正弘

    コ・メディカル形態機能学会第7回学術集会　2008/09/13

  78. ラット皮膚感染潰瘍におけるバイオフィルム形成と上皮化の関連性

    菅野恵美, 張 連波, 鳥谷部荘八, 館 正弘

    第10回日本褥瘡学会学術集会　2008/08/29

  79. 皮膚創傷治癒過程における好中球の集積とTNF-α産生：緑膿菌接種の影響

    立 雅恵, 菅野恵美, 鳥谷部荘八, 石井恵子, 丸山良子, 川上和義, 館 正弘

    第73回日本インターフェロン・サイトカイン学会学術集会、第19回日本生体防御学会学術総会、第45回補体シンポジウム 3学会合同大会　2008/07/10

  80. Contribution of Quorum Sensing to the formation of Peudomonas aeruginosa biofilms on skin wounds International-presentation

    Emi Kanno, Masahiro Tachi, Lianbo Zhang, Sohachi Toriyabe

    World Union of Wound Healing Aocieties Meeing 2008　2008/06/04

  81. 褥瘡初期に出現するCD34陽性細胞に関する免疫組織化学的検討

    伊達文子, 菅野恵美, 山下雅大, 渡辺皓, 小野栄夫

    第97回日本病理学会総会　2008/05/15

  82. 創洗浄によるバイオフィルム抑制効果の検討

    菅野恵美, 館 正弘, 張 連波, 鳥谷部荘八, 小阪智大

    第4回 日本褥瘡学会 東北地方会　2008/02/09

  83. 希釈ポビドンヨード溶液を用いた創洗浄の検討

    菅野恵美, 館 正弘, 張 連波, 鳥谷部荘八, 小阪智大

    第37回 日本創傷治癒学会　2007/12/06

  84. ラット皮膚潰瘍における緑膿菌バイオフィルム形成 – Las？、Rhl？遺伝子欠損の影響に注目して？

    張 連波, 館 正弘, 菅野恵美, 鳥谷部荘八, 小阪智大

    第37回 日本創傷治癒学会　2007/12/06

  85. 皮膚潰瘍におけるバイオフィルムの観察

    鳥谷部荘八, 館 正弘, 菅野恵美, 今井啓道, 武田 睦, 工藤宏之, 古和田雪, 宮地智洋, 小阪智大

    第16回 日本形成外科学会基礎　2007/10/11

  86. ラット皮膚潰瘍創における緑膿菌バイオフィルムの微細構造

    菅野恵美, 鳥谷部荘八, 館 正弘

    コ・メディカル形態機能学会 第6回学術集会　2007/09/15

  87. 皮膚潰瘍創における緑膿菌バイオフィルム

    菅野恵美, 鳥谷部荘八, 館 正弘

    第3回日本褥瘡学会東北地方学術集会　2007/05/26

  88. Biofilm formation by pseudomonas aeruginosa on skin wounds: an in vivo model International-presentation

    Emi Kanno, Sohachi Toriyabe, Ryoko Maruyama, Masahiro Tachi

    EWMA 2007　2007/05/02

  89. Experimental wound ischemia does not increase the formation of biofilms International-presentation

    Masahiro Tachi, Sohachi Toriyabe, Emi Kanno

    EWMA 2007　2007/05/02

  90. Morphological study on the blood supply in cadaver sacral region -Comparison between average physique and emaciation- International-presentation

    Emi Kanno, Yumi Matsuda, Ryoko Maruyama, Hiroshi Watanabe

    Experimental Biology 2007　2007/04/28

  91. ラット皮膚潰瘍における緑膿菌バイオフィルムモデルの作成‐虚血とバイオフィルム形成について‐

    鳥谷部荘八, 館 正弘, 菅野恵美

    第36回日本創傷治癒学会学術集会　2006/12/04

  92. 皮膚潰瘍創における細菌バイオフィルム形成の検討-虚血の影響に注目して-

    菅野恵美, 鳥谷部荘八, 館 正弘

    第5回コ・メディカル形態機能学会 学術集会　2006/09/23

  93. ショートステイ的短期入院における患者、介護者の満足度と課題

    鈴木加恵, 大里るり, 保原奈央美, 菅野恵美, 松田みち子

    第10回日本難病看護学会学術集会　2005/09/02

  94. 褥瘡好発部位としての仙骨部における血液供給-動脈の由来と走行に注目して-

    菅野恵美, 渡辺 皓

    第6回北日本看護学会学術集会　2002/09/07

  95. 褥瘡好発部位としての仙骨部の解剖学的特徴

    菅野恵美, 渡辺 皓

    第4回日本褥瘡学会学術集会　2002/08/30

  96. 仙骨部皮膚組織の構造

    菅野恵美, 渡辺 皓

    第3回日本褥瘡学会学術集会　2001/09/21

  97. 解剖実習遺体にみられた褥瘡に関する研究

    菅野恵美, 渡辺 皓

    第5回北日本看護学会学術集会　2001/09/15

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Industrial Property Rights 2

  1. 皮膚塗布用の皮膚創傷治癒促進組成物、その製造方法、及び創傷被覆材

    特許7045671

    Property Type: Patent

  2. 皮膚損傷治癒の促進剤

    Property Type: Patent

Research Projects 46

  1. 皮膚の常在菌を活用したアトピー性皮膚炎のバリア機能を高める革新的技術の創出

    菅野 恵美, 丹野 寛大, 佐藤 佑樹, 庄司 未樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2025/06/27 - 2027/03/31

  2. Elucidation of the mechanism of excessive inflammation control in the early stage of pressure ulcer by hot and cold compresses and development of nursing care with multidisciplinary professionals

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Yamagata University

    2024/04/01 - 2027/03/31

  3. 肥満改善後も慢性炎症を増悪させる肥満メモリー免疫細胞の探索と創傷難治化への関与

    丹野 寛大, 菅野 恵美, 佐藤 佑樹, 伊師 森葉, 庄司 未樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2024/06/28 - 2026/03/31

  4. Development of an innovative treatment device using electrosterilization for wounds with infection

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: The University of Tokyo

    2024/06/28 - 2026/03/31

  5. 熱傷治療に新たな選択肢を-感染制御と上皮化誘導を両立するナノ型乳酸菌-

    山口 賢次, 菅野 恵美, 丹野 寛大, 佐藤 光, 工藤 大介, 今井 啓道, 庄司 未樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2023/04/01 - 2026/03/31

    More details Close

    本年度は急性創傷におけるナノ型乳酸菌の効果の検証と熱傷創モデルの確立を中心に研究を進めた。 急性創傷におけるナノ型乳酸菌の効果に関しては、直接投与の濃度を検証し、さらに初回単回投与での創閉鎖率の改善を認めた。加えて、病理学的解析においても、CD31陽性血管数やαSMA陽性筋繊維芽細胞数の増加を認め、創傷治癒に関わる肉芽の増生を認めた。上皮化に関しても、上皮の進展率の上昇を認め、増生した肉芽上に皮膚が進展し、治癒が促進していることを確認できた。 急性期において炎症の調節に必要なサイトカインに関してELISA法での解析を行ったところ、IL-6やTNF-αの早期での上昇を認めた。加えて、好中球の遊走に関与するCXCL2やCXCL1なども著明な上昇を認めた。これらは、乳酸菌を急性創傷に投与することで創部における炎症が惹起され、創傷治癒過程における炎症期のメカニズムと合致する。炎症を惹起することで創の清浄化が促進する可能性が示唆された。さらに、抗炎症に作用するI L-10も後期にて上昇を認めた。炎症期から増殖期への移行を適切にかつ早期に誘導することで、創傷治癒を早めている可能性がある。今後、フローサイトメトリーで創部に遊走される白血球の分画を解明し、創傷治癒過程の変化を観察する予定である。 熱傷創モデルに関しては、マウス背部に電気ごてで作成した熱傷創の治癒過程及び病理学的変化を解析した。熱傷創が治癒する過程と、温度による変化、保護剤による違いなどを検討している。今後、標準的な治癒過程を明らかにし、ナノ型乳酸菌投与による変化を比較検討していく予定である。

  6. 「食べる」を支える、がん治療に伴う粘膜障害に対する新規ケア法の創出

    菅野 恵美, 丹野 寛大, 佐藤 佑樹, 佐藤 光, 庄司 未樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2024/04/01 - 2025/03/31

  7. 消化管をケアし、がん治療に伴う栄養障害を改善する革新的技術の創出

    菅野 恵美, 丹野 寛大, 佐藤 佑樹, 佐藤 光, 庄司 未樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2023/06/30 - 2025/03/31

  8. Snake venom heals wounds -Inhibition of NETs by batroxobin and induction of CD34positive cells-

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2022/04/01 - 2025/03/31

  9. 「食べる」を支える、がん治療に伴う粘膜障害に対する新規ケア法の創出

    菅野 恵美, 丹野 寛大, 佐藤 光, 庄司 未樹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

  10. 褥瘡の再発を防ぐナノ型乳酸菌を含有した創傷被覆材の創出

    菅野 恵美

    Offer Organization: 国立研究開発法人 日本医療研究開発機構（AMED）

    System: 令和4年度「官民による若手研究者発掘支援事業（社会実装目的型の医療機器創出支援プロジェクト）」

    2022/04 - 2024/03

  11. 糖尿病性足壊疽に対する切り札-プロタミン含有創傷被覆材の開発-

    梅山 広勝, 菅野 恵美, 丹野 寛大, 高木 尚之, 山口 賢次

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2021 - 2024/03

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    本研究はサケ白子由来抽出物プロタミンを使用した創傷治癒に関する解析である。プロタミンは抗凝固薬であるヘパリンの拮抗薬やインスリンの作用持続薬として実臨床で使用されており、今回さらなる新規治療薬としての効果を解析することが目的である。in vitroの解析ではマウスから抽出した骨髄由来樹状細胞を培養し、プロタミンで刺激する実験を行った。その結果、IL-6やTNF-α、IL-12p40などの炎症性サイトカインの上昇を認めた。創傷治癒において早期の炎症性サイトカインの上昇は白血球の遊走を促進し、創の清浄化を早め治癒を促進すると言われている。今後は、より後期のフェーズで炎症が低下し、創傷治癒における増殖期へのスムーズな移行がなされているかをフローサイトメトリーなどで解析する必要がある。また、in vivoの解析において、マウスへのプロタミン投与と至適濃度の解析を行った。非固着性ガーゼに塩酸プロタミンを浸漬させ乾燥させた疑似的な創傷被覆材を作成し、マウス背部に3mmパンチで作成した全層皮膚欠損に貼付、連日交換のうえDay3で創閉鎖率を解析した。結果、1.25mg／mlの溶液で作成した擬似的被覆材において創閉鎖率の改善を認めた。in vitroの解析で高濃度での細胞毒性の可能性が指摘されていたため、本実験で示された濃度を至適濃度として今後解析を進めていく必要がある。今後、さらに被覆材に準じた形で交換の頻度や解析日、さらに創サイズの変更や感染創、糖尿病マウスでの解析などを検討している。

  12. シリコン人工乳房へのバイオフィルム形成に注目した慢性炎症機序の解明、制御法の開拓

    高木 尚之, 菅野 恵美, 山口 賢次

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2021 - 2024/03

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    乳癌術後の乳房再建術において、組織拡張期(TE)や人工乳房(SBI)を用いた手術が広く行われているが、これらのシリコン性の人工物周囲には、長期的な被膜拘縮を高頻度で生じ、さらに近年ブレストインプラント関連未分化大細胞型リンパ腫(BIA-ALCL)の発声が報告されている。また、被膜拘縮は表面が平滑なスムースタイプのシリコンで多いのに対し、BIA-ALCLは表面に微細な凹凸のあるテクスチャードタイプのシリコンで多いとされており、シリコンの表面形状による反応の違いが指摘されているが、これらの原因は全く解明されておらず、深刻な合併症に対する対策は立てようがない。 申請者らはこれまで乳房再建において43臨床検体のテクスチャードタイプのシリコン周囲被膜を採取し、驚くべきことに51%の検体でバイオフィルムが検出されるという結果を示した。 本研究では、これまで本邦で主流であったテクスチャードタイプのシリコンから、BIA-ALCLを契機に使用が開始されたスムースタイプのシリコン周囲の被膜検体を同様に検討することで、炎症遷延化の原因の一つと考えられているバイオフィルム形成がシリコンの表面形状の違いにどのように関与するかを解析することを目的としている。 以上よりバイオフィルムによるシリコンインプラントへの影響について基盤情報を獲得し、さらに被膜の肥厚化・拘縮に対してはどのような生体反応が関与しているのかを明らかにする。

  13. 慢性創傷に対する「好中球細胞外トラップ」阻害によるユニバーサル創傷ケアの開発

    丹野 寛大, 菅野 恵美, 高木 尚之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2021 - 2024/03

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    慢性創傷は、バイオフィルム形成、血流障害などが複合的に関与し、難治化する。しかし、複数の原因に対し、同時にアプローチできる創傷ケアは存在しない。難治性の原因によらず、全ての創傷を治癒に導く「ユニバーサル創傷ケア」が求められている。 本研究では、既に突発性難聴や慢性閉塞症などの治療薬として用いられ、血流改善効果や血栓を形成する好中球細胞外トラップ阻害効果が確認されているデフィブラーゼが創傷治癒に与える影響を解明することにより、ユニバーサル創傷ケアの確立を目指す。 急性創傷として、マウス背側皮膚に直径6 mmの全層欠損層を4個作成し、生理食塩水(生食)またはデフィブラーゼ 30 BU/kgを腹腔内に投与した後、創閉鎖率、再上皮化率、肉芽面積、血管新生の解析を行った。さらに、慢性創傷モデルとして、我々が確立したα-mannan投与モデルを用い、創作成後、生食またはデフィブラーゼ30 BU/kgを腹腔内に投与し、創閉鎖率の解析を行った。 急性創傷モデルでは、デフィブラーゼ投与により、創作成後3, 5, 7日目の創閉鎖率が有意に増加した。デフィブラーゼ投与による再上皮化率には差は認められなかったが、肉芽面積は創作成後7日目に有意に増加した。さらに、デフィブラーゼ投与により創作成後5日目の血管数が有意に増加した。 慢性創傷モデルもデフィブラーゼ投与により、創作成後5日目に創閉鎖率が生食投与群と比較し、有意に増加した。

  14. 飲む褥瘡予防法の確立に向けた、皮膚－腸管ネットワークによる創傷治癒制御機構の解明

    菅野 恵美

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的研究(萌芽)

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2021/07 - 2023/03

  15. Biofilm形成に関わる免疫と細菌間クロストークの解明、新規治療法の開発

    下寺 佐栄子, 館 正弘, 菅野 恵美, 丹野 寛大, 高木 尚之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2020/04 - 2023/03

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    本課題において申請者らは、褥瘡や糖尿病性下腿潰瘍など難治性皮膚潰瘍におけるBiofilm形成への免疫と細菌間クロストークの関与について解明し、宿主由来のBiofilm誘導因子をターゲットとした新規治療法の確立を目指している。特に、好中球がBiofilm誘導に関与する可能性に注目している。創傷治癒において、好中球は最も早く創部に到達し、初期の感染防御や炎症反応に重要な役割を担う。その一方でBiofilmを伴う皮膚潰瘍では、好中球による組織障害が報告されており、好中球の暴走が慢性的感染症のトリガーではないかと指摘されている。 我々は昨年度、SDラットを用いて皮膚潰瘍緑膿菌Biofilm形成モデルを確立し、①皮膚潰瘍への緑膿菌接種により、24時間をピークに好中球集積数が高まり、その後低下すること、②好中球集積には、緑膿菌クオラムセンシング分子が関与することを明らかにした。 今年度は、緑膿菌クオラムセンシング分子の合成に関わるlasI遺伝子を欠損した緑膿菌とその親株であるPAO1を用いて、lasI遺伝子が好中球集積に関わるか否かについて検証を行った。PAO1株と比較してlasI遺伝子を欠損した緑膿菌株では、創部への好中球集積が約半数に減少した。また、好中球の誘導に関わるサイトカインであるTNF-αは約3分の1の産生量に減少した。次にlasI遺伝子が産生に関わるクオラムセンシング分子である3-oxo-C12-HSLを創傷部位に投与したところ、TNF-α産生を伴い、創傷部位に大量の好中球集積を認めた。 以上のことから、緑膿菌クオラムセンシング分子3-oxo-C12-HSLは、好中球の遊走、TNF-α産生の増加に関与することが明らかになった。今後、好中球と緑膿菌クオラムセンシング分子のクロストークについて解析を進める予定である。

  16. Nursing care approach via group3 innate lymphoid cells to orchestrate pressure-related wounds with deep tissue injury repair.

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Yamagata University

    2020/04 - 2023/03

  17. Development of immunotherapy through C-type lectin receptors for chronic wounds

    TACHI Masahiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2019/04 - 2022/03

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    C-type lectin receptors (CLRs) recognize microbial polysaccharides. The precise roles of these CLRs in wound healing remain unclear. This study was conducted to determine the roles of Dectin-1 and Dectin-2 in wound healing, with a particular focus on the kinetics of neutrophilic inflammatory response. Wounds were created on the backs of C57BL/6 mice, and the effects of Dectin-1 or Dectin-2 deficiency. We also analyzed wound closure, histological findings, and neutrophilic inflammatory response, including neutrophil extracellular trap formation. We found that Dectin-1 contributed to the acceleration of wound healing by inducing early-phase neutrophil accumulation, whereas Dectin-2 was involved in prolonged neutrophilic responses and neutrophil extracellular trap formation, leading to delayed wound healing. These results suggest that Dectin-1 and Dectin-2 have different roles in wound healing through their different effects on the neutrophilic response.

  18. Development of wound care for regulating inflammatory responses using nano-type lactic acid bacteria

    Kanno Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2019/04 - 2022/03

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    The purpose of our study is to develop an “innovative skin wound care using nano-type lactic acid bacteria (heat-killed KH2).” We analyzed percent wound closure and granulation area. We found that heat-killed KH2 contributed to the acceleration of the formation of granulation tissue by inducing tumor necrosis factor-α, interleukin-6, basic fibroblast growth factor, transforming growth factor (TGF)-β1 production. In addition, heat-killed KH2 also improved wound closure, which was accompanied by the increased production of TGF-β1 in diabetic mice. Topical administration of heat-killed KH2 might have therapeutic potential for the treatment of chronic skin wounds in diabetes mellitus. In the present study, we concluded that heat-killed KH2 promoted skin wound-healing through the formation of granulation tissues and the production of inflammatory cytokines and growth factors.

  19. Contribution of invariant natural killer T cells and IFN-gamma to neutrophilic inflammation in skin wounds

    Kanno Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2019/06 - 2021/03

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    Interferon (IFN)-γ is mainly secreted by CD4+ T helper 1 (Th1), natural killer (NK) and NKT cells after skin injury. Although IFN-γ is well known regarding its inhibitory effects on collagen synthesis by fibroblasts in vitro,information is limited regarding its role in wound healing in vivo.In the present study, we analyzed how the defect of IFN-γ affects wound healing. Full-thickness wounds were created on the backs of wild type (WT) C57BL/6 and IFN-γ-deficient (KO) mice. We analyzed the percent wound closure, accumulation of leukocytes, and matrix metalloproteinases (MMPs). IFN-γKO mice exhibited significant attenuation in wound closure on Day 10, characteristics that are associated with prolonged neutrophil accumulation. Expression levels of MMP-2 mRNA were significantly greater in IFN-γKO than in WT. Moreover, under neutropenic conditions created with anti-Gr-1 monoclonal antibodies, wound closure in IFN-γKO mice was recovered through low MMP-2 expression levels.

  20. Contribution of C-type lectin receptor-mediated signaling to neutrophil extracellular traps in skin ulcers

    Akiko Yano

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2018/04 - 2021/03

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    C-type lectin receptors recognize microbial polysaccharides. The C-type lectin receptors such as dendritic cell-associated C-type lectin (Dectin)-1 and Dectin-2, which are triggered by β-glucan and α-mannan, respectively, contribute to upregulation of the inflammatory response. This study was conducted to determine the roles of Dectin-1 and Dectin-2 in skin wound healing, with a particular focus on the kinetics of neutrophilic inflammatory response. Full-thickness wounds were created on the backs of C57BL/6 mice, and the effects of Dectin-1 or Dectin-2 deficiency and those of β-glucan or α-mannan administration were examined. We also analyzed wound closure, histological findings, and neutrophilic inflammatory response, including neutrophil extracellular trap formation. We found that Dectin-2 was involved in prolonged neutrophilic responses and neutrophil extracellular trap formation, leading to delayed wound healing.

  21. 深部損傷褥瘡（DTI）における炎症誘導機序の解明と治癒促進ケア技術の確立

    松田 友美, 石田 陽子, 菅野 恵美, 櫻田 香, 片岡 ひとみ

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 山形大学

    2017/04 - 2021/03

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    本研究は、深部損傷褥瘡（Deep Tissue Pressure Injury: DTPI）の炎症誘導機序の解明とDTPI発生初期およびDTPIの治癒調整・促進の適切な看護ケア技術の開発を行うことを目的としている。これまでの好中球の枯渇実験により、好中球の適正な時期の活性がない場合は、ケモカイン（KC）が持続的に増加し、炎症性サイトカイン（MCP-1，IL-1β、TNF）などの増加活性の遅延をもたらしており、炎症期が遷延することが明らかになった。そこで、今年度は好中球の適正な時期の活性を促進する刺激として、臨床で一般的に用いられる冷・温罨法を実験群に施した。その結果、冷・温罨法を施していない対照の圧迫創は、3日目以降に、IL-1β、TNF-α、IL-17Aが増加を示した。冷罨法はIL-1β、TNF-α、IL-17A、IL-10いずれも創傷作製後1日目にピークを迎えその後数値が低下するのに対し、温罨法は3日目、7日目に数値が上昇することが明らかになった。好中球遊走を促進するIL-17Aの増加は、炎症を抑制するIL-10と連動しており、マクロファージを活性化するIL-1βも促進されていることが示された。冷罨法は、比較的炎症性サイトカインの上昇はなく、温罨法は炎症性サイトカインの上昇が認められたものの、肉眼的には冷罨法の創は痂疲が顕著で収縮が認められ、温罨法は表層の創傷の程度は悪化せずに経過した。圧迫創において、温罨法は炎症系サイトカイン、抗炎症系のサイトカイン双方の上昇を認め、好中球の遊走刺激になることが考えられた。サイトカインは上昇したものの、拮抗するサイトカインの上昇が認められたことから炎症のバランスを保っているとも考えられ、肉眼的な創傷の程度が軽傷であったことから、温罨法は圧迫創の表層部における創傷治癒調整に効果をもたらす可能性が示唆された。

  22. The right lateral decubitus position may attenuate sympathetic nervous activity in elderly adults

    Maruyama Ryoko, HOSHI Junko, SUN Xinru

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2017/06/30 - 2019/03/31

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    This study was to evaluate the effect of the spine, left lateral decubitus, and right lateral decubitus position on autonomic nervous activity elderly by using spectral analysis heart rate variability (HRV). Forty-five elderly adults were enrolled. The lowest heart rate continued for 10 min, when participants were left lateral decubitus position on compared with the other two positions, The low frequency HRV to high-frequency HRV ratio (LF/HF) for the right lateral decubitus position was significantly lower than that for the other position. The right lateral decubitus position may attenuate sympathetic nerve activity in elder adults.

  23. Involvement of NETosis on the diabetic ulcers and development of novel wound care

    Tachi Masahiro, KAWAKAMI kazuyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2017/06 - 2019/03

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    It is well known about immunocompromised state and neutrophil dysfunction in diabetic foot ulcers. But the detailed mechanism of neutrophil dysfunction remains unclear. In the current study, to identify the possible contribution of NETosis the co-localization between Ly6G (marker of neutrophils) and citrullinated histon (an indicator of NETosis) and elastase activity after α-mannan administration were examined. We discovered that the expression of citrullinated histone was accerated at the wound sites. In addition, elastase activity level was significantly higher in α-mannan-administrated mice than in vehicle-administered mice. We next investigated the effect of sivelestat sodium, a neutrophil elastase inhibitor, on wound closure in WT mice administered α-mannan, and the administration of sivelestat sodium was found to significantly improve the delayed wound closure caused by α-mannan.

  24. Elucidation of environment in wound with biofilms and development of efficient therapies

    Takagi Naoyuki, TACHI Masahiro, KAWAKAMI Kazuyoshi, KANNO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2017/04 - 2019/03

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    The involvement of biofilms in chronic wounds is becoming widely known, but all chronic wounds don't have biofilms.In this study, biofilm formation in chronic wounds was 33%. In addition, it was found that biofilm formation in ischemic conditions was inhibited, and that biofilm formation and neutrophils were involved. The possibility of inhibiting the biofilm formation by negative pressure closure therapy (NPWT-id) among the therapies for various biofilms was indicated.

  25. Development of innovative wound care using applied immune regulation on chronic wounds

    Tachi Masahiro, KAZAMA itsurou

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2016/04 - 2019/03

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    In chronic wounds, the inflammatory responses are prolonged with persistent neutrophil infiltration. Recently, we showed that skin wound healing was delayed and the healing process was impaired under conditions lacking invariant natural killer T (iNKT) cells, an innate immune lymphocyte with potent immuno-regulatory activity. In the present study, to identify the possible contribution of NKT cells activation, the effects of α-Galactosylceramide (α-GalCer) administration on wound healing, specific activator of iNKT cells, were examined. Administration of a-GalCer at the wound sites significantly promoted wound closure, granulation tissue formation, angiogenesis compared with vehicle treatment. In addition, the inflammatory cells were rapidly decreased and granulation tissue were enhanced in α-GalCer administered mice.

  26. Analysis of DAMPs at the wound sites and development of novel wound care on chronic wounds

    Emi Kanno

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2016/04 - 2019/03

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    The role of endogenous damage-related molecular patterns (DAMPs) in skin wound healing remains unclear. In the present study, to identify the possible contribution of CLRs, such as Dectin-2, the expression of Dectin-2 mRNA was examined. Dectin-2 mRNA expression reached peak levels at 12 hours in WT mice, and expression levels declined thereafter to the basal level. we found that both neutrophils and macrophages expressed Dectin-2, and the proportions of these cells expressing Dectin-2 were 40.1±5.0% and 32.1±2.9%, respectively. In immunohistochemical analysis, DAMPs recognized by Dectin-2 was increased in infiltrating cells at the wound site.

  27. Age differences in cardiac autonomic nervous regulation in response in change positions.

    Maruyama Ryoko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    The aim of this study was to evaluate the effect of the spine, left lateral decubitus, and right decubitus position on autonomic nerve activity in young adults and elderly by using spectral analysis of heart rate variability(HRV). After laying in spine position, all participants in a random order and maintained the position for 10 min, while ECG data were recorded to measure HRV. The lowest heart rate continued for 10 min. when participants were in the left decubitus position compared with the other positions, however the HRV remained unchanged. The low frequency to high frequency ratio for the right lateral position was significantly lower than that for the other positions. The right lateral position may attenuate sympathetic nerve activity in elderly adults.

  28. Analysis of inframmatory chain mechanism at the wound site through C type lectin receptor (Mincle)

    Kei Yoshikawa, KAWAKAMI Kazuyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2016/04 - 2018/03

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    Macrophage inducible C-type lectin (Mincle) is an activating receptor, expressed in macrophages and dendritic cells, which senses PAMPs and DAMPs. However, the role of Mincle at the wound sites has not been elucidated. In the present study, we addressed a question how defect of Mincle affected to the healing process of wounds. Wounds were created on the backs of wild type (WT) C57BL/6 and Mincle-deficient (KO) mice. We analyzed percent wound closure, height of granulation tissue, expression of Mincle by real-time PCR and immunohistochemistry. The percent wound closure was significantly increased in Mincle KO mice compared to WT mice, which was associated with the increased height of granulation tissues. Mincle was detected at the early phase after wound creation. These results suggest that Mincle may be involved in the regulation of granulation at the wounded tissues, which may lead to delayed wound healing.

  29. The cardiac autonomic nervous response to elderly adults to position change.

    Maruyama Ryoko, Sasaki Kounosuke, Bao Sarina

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2017/03/31

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    In particular, parasympathetic nerve activity as well as arterial baroreflex sensitivity reduces with aging process. In elderly patients with coronary artery disease, high parasympathetic nerve activity and low sympathetic nerve activity have been observed in right lateral decubitus position compared with supine and left lateral decubitus positions. However, little research has been conducted to determine sex and age differences in autonomic nervous activity in different positions, i.e., supine, left recumbent, and right recumbent positions. The aim of this study was to evaluate the effect of the left and right recumbent positions on heart rate variability in elderly was the lowest among the three positions regardless of sex. In elderly men, the LF/HF ratio in right recumbent position was significantly lower than that in supine position. Right recumbent position may attenuate cardiac sympathetic nerve activity in elderly adult men.

  30. The analysis of skin-liver network during skin wound healing

    Yamauchi Sakiko, KAWAKAMI KAZUYOSHI, KANNO EMI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04 - 2017/03

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    Recently, the interaction between multiple organs and tissues have been clarified. However skin-liver network have not been reported. In the present study, we analyzed the network between skin and liver after wound creation. Hepatic NKT cells and neutrophils were increased after wounding. In addition, MIP-2 expression was also increased after wounding. These results suggest that skin-liver network may be existed.

  31. Involvement of type I interferons on the wound healing in skin

    Takayuki Aizawa, KAWAKAMI Kazuyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04 - 2017/03

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    Type I interferons (IFNs) are secreted by many cells upon stimulation via pattern recognition receptors like TLR3 and TLR7, and deliver the activation signal through a specific cell surface IFN-α/β receptor (IFNAR) that consists of IFNAR1 and IFNAR2 chains. Although type I IFNs are well known in their antiviral activity, limited information is available in their role during wound healing. In the present study, we analyzed how defect of IFNAR-mediated signaling affected the wound healing . Wounds were created on the backs of C57BL/6 mice, we examinesd the percent wound closure and accumulation of leukocytes were compared between IFNAR1 gene-disrupted (KO) mice and wild type mice. The percent wound closure was significantly delyed in IFNAR1KO mice compared to WT mice on day 5, which was associated with decreased macrophage accumulation. These results suggest that type I IFNs may be involved in the regulation of the wounds healing.

  32. Elucidation of IL-17 function and production in the wound healing and the role in chronic wounds

    TAKEDA ATSUSHI, TAKAGI Naoyuki, TANNO Hiromasa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04 - 2017/03

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    Interleukin-17 (IL-17) is a proinflammatory cytokine. The role of this cytokine in the wound healing process is not fully clarified. In this study, IL-17A knockout mice showed promoted wound healing and decreased neutrophil accumulation compared with wild-type mice, and the administration of recombinant IL-17A led to delayed wound closure, accelerated neutrophilic accumulation and improved wound repair with treatment of a neutrophil elastase inhibitor. Neutrophils, macrophages and gamma-delta T cels showed IL-17A expression after wounding in wild-type mice. These results indicated that IL-17A hampared the wound healing process and neutrophilic inflammation caused by IL-17 led to impaired wound healing in skin.

  33. Relation birth weight and adult hypertension on ethnic minorities In China

    Maruyama Ryoko, KANNO Emi, MINAMI Yuko, TANNO Hiromasa, BAO Sarina

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

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    Low birth weight(LBW) conformed as a risk of high blood pressure in later life.However, a number of studies have shown that Mongolian people have higher prevalence of hypertension compared to other ethnic groups. including the Japanese. The purpose of this study was to identify whether there are differences in the relationship of LBW and the risk of hypertension between Japanese and Mongolian.We measured BP and heart rate variability at resting condition and postal change for both Japanese and Mongolian volunteers with LBW and normal birth weight,respectively. We conclude that Mongolian young adults a higher risk of hypertension compared to the Japanese counterpart.

  34. The effects of DAMPs through C type lectin receptors on the wound healing

    Usuba Chie, TACHI Masahiro, KANNO Emi, KAWAKAMI Kazuyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2014/04 - 2016/03

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    We addressed the possible involvement of C-type lectin receptors (CLRs) in the wound site. The expression of CLRs (Mincle, Dectin-1, Dectin-2) mRNA in the wounded skin were increased after wound creation. We analyzed the role of caspase recruitment domain-containing protein 9 (CARD9), which is an essential signaling adaptor molecule in NF-κB activation upon triggered through CLRs. The percent wound closure, accumulation of leukocytes, mRNA expression of TNF-α were decreased in CARD9 KO mice. These results suggest that CLRs may involved in the inflammatory response at the wound site.

  35. Analysis of inhibition of MRSA infection by quorum sensing molecules from pseudomonas aeruginosa

    Tachi Masahiro, KANNO Emi, TAKEDA Atsushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2014/04 - 2016/03

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    We addressed the possible involvement of quorum sensing (QS) molecules, 3-oxo-C12-HSL and C4-HSL, from Pseudomonas aeruginosa to inhibition of methicillin-resistant Staphylococcus aureus (MRSA). We used Vancomycin (VCM) as a positive control. More than 10% Dimethyl sulfoxide (DMSO) had the effect of inhibition of MRSA, but on the other hand QS molecules had not that effect. In addition, the MRSA growth did not affect by the stimulation of 3-oxo-C12-HSL and C4-HSL at the same time. Minimum inhibitory concentration of VCM showed 1 μg/ml. These results suggest that QS molecules, 3-oxo-C12-HSL and C4-HSL, have not effect to inhibition of MRSA.

  36. Promoted Nursing Care of Wound Healing Based on Mechanisms of The Deep Tissue Injury (DTI)

    MATSUDA YUMI, KANNO EMI, ISHIDA YOKO

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Yamagata University

    2013/04 - 2016/03

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    In the present study, we evaluated the effects that cool and hot packs as an initial therapy has contributed wound healing on post-pressure induced deep tissue injury model mouse. Wound healing is similar to that of secondary intention on the model mouse. The cool group had significant tissue necrosis in the epidermis/dermis, but l though the deep skeletal muscle had been damaged, muscle cell regeneration was noted on days 5 to 7. The hot group exhibited reverse findings in the respective tissue layers, and had delayed regeneration of skeletal muscle. Applied cool or hot packs to the pressure wound were indicated different effects on each layer of the skin tissue including muscles.

  37. Involvement of DAMPs in the inflammatory response at the chronic wound site and the establishment of effective care

    Kanno Emi, TACHI Masahiro, MARUYAMA Ryoko, KAWAKAMI Kazuyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2013/04 - 2016/03

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    We addressed the possible involvement of damage-associated molecular patterns (DAMPs) and C-type lectin receptors (CLRs)in the wound site. We analyzed the role of caspase recruitment domain-containing protein 9 (CARD9), which is an essential signaling adaptor molecule in NF-κB activation upon triggered through CLRs. Next, we analyzed the expression of SAP130 and Mincle in the wound site. Wounds were created on the backs of wild-type and CARD9 gene-disrupted (knockout) mice. We analyzed percent wound closure, accumulation of leukocytes, mRNA expression of cytokines and chemokines by real-time PCR. CARD9 KO mice were exhibited significant attenuation in wound closure compared with WT mice, which was associated with the decreased expression of TNF-α, MIP-1α and MIP-1β mRNA.In an immunohistochemical analysis using WT mice, expression of SAP130 and Mincle were detected in the leukocytes. These results suggest that DAMPs may be involved in the inflammatory response.

  38. Hemodynamic responses to posture change in elderly subjects

    MARUYAMA Ryoko, KANNO Emi, SASAKI Kounosuke, SATO Haruka, BAO Sarina

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04/01 - 2015/03/31

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    Little research has been conducted on age-related differences in autonomic nervous activity and circulation between the various recumbent positions. The purpose of the present study was to evaluate elderly subjects show differences in the autonomic nervous activity and circulation between the 3 positions (supine, right lateral decubitus, and left lateral decubitus position). We performed electrocardiographic measurements for 50 elderly subjects (63-85 years). No significant differences were observed in mean BP, low frequency HRV, high frequency HRV, and their ratio. However, in the left lateral decubitus position, HR was significantly lower than in the supine position. In contrast, the LF/HF ratio in the right lateral decubitus position was significantly lesser than that in the supine position the elderly .

  39. Explication of NKT cellular function in a chronic hurt and establishment of a new remedy by the control

    KOWATA Yuki, TACHI Masahiro, KAWAKAMI Kazuyoshi, KANNO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2012/04 - 2015/03

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    Natural Killer T (NKT) cells, an unique lymphocyte with a potent regulatory ability in various inflammatory responses, produce IFN-γ and IL-4 upon stimulation with α-galactosylceramide (α-GalCer). However, it is not understood how this lymphocyte is involved in skin wound healing. In this study, we elucidated the role of NKT cells in the skin wound healing process. In Jα18KO mice lacking NKT cells, wound healing was impaired compared with wild-type (WT) mice. The adoptive transfer of liver mononuclear cells (LMNCs) from WT, but not from Jα18KO mice, resulted in the reversal of this impaired wound healing in Jα18KO mice. Administration of α-GalCer resulted in the acceleration of wound healing. These results indicate that NKT cells play important roles in wound healing.

  40. Low-energy extracorporeal shock wave therapy enhances skin wound healing in diabetic mice: a critical role of endothelial nitric oxide synthase.

    IMAI Yoshimichi, ITOU Kenta, KAWAKAMI Kazuyoshi, TAKEDA Atsushi, KANNO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2011 - 2013

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    There is the country where low power outside the body shock wave treatment is really used for for a diabetic foot ulcer and a chronic leg ulcer in the Western European countries, but the authorization in this country is not done. The fundamental law can expect the role as the ace in the hole in the case that revascularizing it has difficult for diabetes, the arterial ischemia-related ulcer caused by the seriously ill ischemic limb (occlusive arteriosclerosis). The vascularization action was clarified through animal experiment. I pushed forward preparations for clinical application in parallel.

  41. The analysis of inflammatory responses and effective care in chronic wounds

    KANNO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2011 - 2012

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    Several bacteria, including Pseudomonas aeruginosa exist in chronic wounds with prolonged inflammatory reaction. In the present study, using a rat model with full-thickness dermal wounds, we examined the effect of P. aeruginosa and quorum sensing (QS) molecules, N-(3-oxododecanoyl)-_L-homoserine lactone (3-oxo-C_<12>-HSL), which is produced by P. aeruginosa. Within 3 days, inoculation with P. aeruginosa or3-oxo-C_<12>-HSL had accelerated local infiltration of neutrophils and tumor necrosis factor-α. These results indicate that 3-oxo-C_<12>-HSL may be involved in induction of inflammatory responses.

  42. The trial of building a nursing assessment for detecting chronic side effects in early stages on radiotherapy.

    SEKIMATA Akiko, MARUYAMA Ryoko, MINAMI Yuko, WATANABE Ikue, KANNO Emi, NAKANO Akihiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    2009 - 2011

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    The screening for detecting genes which involved in breast cancer development revealed 25 related ones. The five of them were unknown as DNA repair factors. Also, we characterized a novel repair gene, MDT1, which we isolated previously, and the experiment showed that MDT1 has a role for the protein metabolism.

  43. New woun d management strategy by distraction of wound biofilm

    TACHI Masahiro, KAWAKAMI Kazuyoshi, KANNO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2009 - 2011

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    Quorum-sensing(QS)systems regulate virulence factors in Pseudomonas aeruginosa, which produces autoinducers. Accerelations of wound healing were not observed when inoculated with ΔlasI or ΔrhlI in place of PAO1 and cancelled when co-treated with these anti HSL antisera, respectively, but not with control rabbit serum. By contrast, administration of HSL significantly accelerated the re-epithelialization. These results suggest that HSL may be deeply involved in the skin wound healing during P. aeruginosa infection. Low-energy extracorporeal shockwave treatment(LE-ESWT)has been demonstrated to accelerate wound repair. E-NOS is induced at the wound tissues by LE-ESWT and plays a critical role in the therapeutic effects of this treatment accelerating the wound healin g through promoting VEGF expression and neo-vascularization.

  44. The analysis in the management of pressure ulcers and chronic wounds

    KANNO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2008 - 2009

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    In the present study, we examined the activity of the silver-containing dressing on wound infection was studied in vivo model. Full- thickness wounds were created on the backs of SD rats. P. aeruginosa strain PAO1 was inoculated at the wound surface. Wounds were harvested at 3 days postwounding, processed for histology and bacterial counts in the wound tissue. The silver-containing dressing did not demonstrate an antibacterial effect in the minimal volume of wound fluid. In conclusion, it is necessary for appropriate measures of the silver-containing dressing to need an accurate acessment of wound environment.

  45. The cardiovascular effect of position change

    MARUYAMAQ Ryoko, IKUE Watanabe, EMI Kannno, MACHIKO Yamamoto, TSUYAKO Sato, TOSHIKO Sugiyama

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2006 - 2008

  46. 褥瘡、慢性難治性皮膚潰瘍に対する局所管理方法の検討

    菅野 恵美

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 若手研究(スタートアップ)

    Category: 若手研究(スタートアップ)

    Institution: 東北大学

    2006 - 2007

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    ラット皮膚潰瘍創実験モデルを用いた細菌と創傷治癒に関する検討 我々が昨年度確立したラット感染性皮膚潰瘍創実験モデル(人工創を作製し、緑膿菌PAO1を接種後、閉鎖環境においた状態)を用い、創傷におけるバイオフィルム形成や細菌と創傷治癒との関連性、創傷における洗浄の効果について検討した。 細菌と創傷治癒との関連性 創作製、細菌接種7日目に組織を摘出し、光学顕微鏡的に観察した結果、緑膿菌を接種した創傷では、好中球浸潤に伴って顕著な表皮の肥厚や表皮化の促進が観察された。このことから細菌のcolonizationにより、通常とは異なる創傷治癒機転が生じ、その際生体側の要因として白血球や表皮細胞の作用が推定された。 創傷における洗浄の効果 潰瘍創作成2日目より、創部を生食にて連日1回/日洗浄した結果、洗浄7日目では洗浄なし群と比較し洗浄群ではバイオフィルムの発達を認めなかった。この結果より、洗浄はバイオフィルム抑制に有効な手段であることを確認した。 創部の細菌感染は創傷治癒遅延原因の一つであるため、創面の細菌数コントロールは重要だと考えられている。本研究により、洗浄が細菌数コントロールに有効であり、潰瘍創に対する洗浄の重要性が確認された。 研究成果の発表 平成19年度に得られた結果のうち、皮膚潰瘍創における緑膿菌バイオフィルム形成や潰瘍創に対する洗浄について、国内外の専門学会にて発表を行うとともに、論文にて公表した。

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Teaching Experience 8

  1. 看護研究原論

  2. 生体機能学Ⅰ・Ⅱ

  3. 医療解剖学

  4. 看護技術論Ⅲ

  5. 看護技術論Ⅱ

  6. 看護技術論Ⅰ

  7. 基礎看護学実習Ⅱ

  8. 基礎看護学実習Ⅰ

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Social Activities 7

  1. [日本国内] 褥瘡管理方法の指導

    2011/10 - Present

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    仙台富沢病院のスタッフを対象に、褥瘡管理方法の実際について伝達した

  2. [日本国内] 褥瘡管理方法の伝達

    2012/04 - 2013/03

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    仙台富沢病院において、褥瘡管理方法の実際について伝達した

  3. [日本国内] 褥瘡管理方法の伝達

    2010/04 - 2011/03

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    仙台富沢病院において、褥瘡管理方法の実際について伝達した

  4. [日本国内] 褥瘡管理方法の指導

    2009/04 - 2010/03

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    仙台富沢病院にて、主に看護師・介護士・栄養士を対象に、褥瘡の局所および全身管理方法に関する情報提供および技術指導を実施

  5. [日本国内] 褥瘡管理方法の指導

    2008/04 - 2009/03

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    仙台富沢病院において、主に看護師・介護士・栄養士を対象とし、褥瘡の局所および全身管理方法について、情報提供と技術指導を実施

  6. [日本国内] 住吉台中学への出前講義

    2008/11/12 -

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    「ひとのからだをみる, 見る, 診る, 看る」というテーマで丸山良子教授の講義後の演習に携わった。

  7. [日本国内] 折立中学校への出前講義

    2008/10/23 -

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    「ひとのからだをみる, 見る, 診る, 看る」というテーマで丸山良子教授の講義後の演習に携わった。

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Media Coverage 2

  1. 『ナノ型乳酸菌を含有した創傷被覆材』の開発

    https://www.tohoku.ac.jp/japanese/2025/05/press20250512-01-amed.html

    2025/05

    Type: Internet

  2. SENDAI NEW PUBLIC

    PR TIMES　(https://prtimes.jp/main/html/rd/p/000000168.000014738.html)

    2021/05

Other 3

  1. 皮膚潰瘍におけるナノ型乳酸菌の効果

  2. 日本褥瘡学会 褥瘡認定師資格 取得

  3. 皮膚潰瘍における緑膿菌接種の影響