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Kohsuke Gonda

Section

Graduate School of Medicine

Job title

Professor

Degree

博士（理学）（筑波大学）
修士（理学）（筑波大学）

researchmap

https://researchmap.jp/gonda555

J-GLOBAL ID

200901067282382917

Research History 6

2020 - Present

International Center for Synchrotron Radiation Innovation Smart (SRIS), Tohoku University
2013 - Present

Tohoku University　Graduate School of Medicine　Professor
2012 - 2013

Tohoku University　Graduate School of Medicine　Associate Professor
2009 - 2012

Tohoku University　Graduate School of Medicine　Lecturer
2005 - 2009

Tohoku University　Graduate School of Medicine　Assistant Professor
2005 - 2008

Tohoku University　Biomedical Engineering Research Organization　Assistant Professor

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Committee Memberships 2

ナノ学会　理事、副会長

2014/04 - Present
日本ナノメディシン交流協会　理事

2011/04 - Present

Professional Memberships 4

THE JAPANESE SOCIETY FOR BIOMATERIALS
THE BIOPHYSICAL SOCIETY OF JAPAN
Society of Nano Science and Technology
THE JAPANESE CANCER ASSOCIATION

Research Interests 12

Synchrotron radiation
drug efficacy
therapy
diagnosis
nanoparticle
fluorescence
X-ray
arteriosclerosis
cancer
imaging
radiology
nanomedicine

Research Areas 3

Life sciences / Radiology /
Life sciences / Tumor diagnostics and therapeutics /
Nanotechnology/Materials / Nanobioscience /

Awards 2

技術賞

2018/06　日本写真学会　蛍光ナノ粒子を用いた高感度タンパク質定量in vitro/in vivoイメージング技術
コニカミノルタ画像科学奨励賞

2010/03　コニカミノルタ画像科学振興財団　In vivo ナノイメージング法を用いた抗がん剤の開発

Papers 89

Development of Gold Nanoparticles Coated with Bismuth Oxide for X-ray Computed Tomography Imaging Peer-reviewed

Soichiro Takiguchi, Ayumi Takahashi, Noriko Yamauchi, Shohei Tada, Takumu Takase, Mone Kimura, Kohsuke Gonda, Yoshio Kobayashi

Journal of Cluster Science　36　(1)　2024/12/02
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s10876-024-02724-8 　

ISSN： 1040-7278

eISSN： 1572-8862
Synthesis of gold nanoparticle-immobilized biodegradable polymer nanoparticles and their degradation properties Peer-reviewed

Keisuke Akino, Ayumi Takahashi, Noriko Yamauchi, Shohei Tada, Takumu Takase, Mone Kimura, Shigeharu Nakao, Kohsuke Gonda, Yoshio Kobayashi

Chemical Papers　78　(5)　3343-3353　2024/02/08
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s11696-024-03315-7 　

ISSN： 0366-6352

eISSN： 2585-7290
Mitochondrial dynamics as a novel treatment strategy for triple-negative breast cancer. International-journal Peer-reviewed

Yuechen Wang, Narumi Harada-Shoji, Narufumi Kitamura, Yuto Yamazaki, Akiko Ebata, Masakazu Amari, Mika Watanabe, Minoru Miyashita, Hiroshi Tada, Takaaki Abe, Takashi Suzuki, Kohsuke Gonda, Takanori Ishida

Cancer medicine　13　(2)　e6987　2024/01

DOI： 10.1002/cam4.6987 　

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INTRODUCTION: Triple-negative breast cancer (TNBC), recognized as the most heterogeneous type of breast cancer (BC), exhibits a worse prognosis than other subtypes. Mitochondria dynamics play a vital role as mediators in tumorigenesis by adjusting to the cell microenvironments. However, the relationship between mitochondrial dynamics and metabophenotype exhibits discrepancies and divergence across various research and BC models. Therefore, this study aims to explore the role of mitochondrial dynamics in TNBC drug resistance and tumorigenesis. METHODS: The Wst-8 test was conducted to assess doxorubicin sensitivity in HCC38, MDA-MB-231 (TNBC), and MCF-7 (luminal). Confocal microscopy and FACS were used to quantify the mitochondrial membrane potential (ΔφM), mitophagy, and reactive oxygen species (ROS) production. Agilent Seahorse XF Analyzer was utilized to measure metabolic characteristics. Dynamin-related protein-1 (DRP1), Parkin, and p62 immunohistochemistry staining were performed using samples from 107 primary patients with BC before and after neoadjuvant chemotherapy (NAC). RESULTS: MDA-MB-231, a TNBC cell line with reduced sensitivity to doxorubicin, reduced ΔφM, and enhanced mitophagy to maintain ROS production through oxidative phosphorylation (OXPHOS)-based metabolism. HCC38, a doxorubicin-sensitive cell line, exhibited no alterations in ΔφM or mitophagy. However, it demonstrated an increase in ROS production and glycolysis. Clinicopathological studies revealed that pretreatment (before NAC) expression of DRP1 was significant in TNBC, as was pretreatment expression of Parkin in the hormone receptor-negative group. Furthermore, low p62 levels seem to be a risk factor for recurrence-free survival. CONCLUSION: Our findings indicated that the interplay between mitophagy, linked to a worse clinical prognosis, and OXPHOS metabolism promoted chemotherapy resistance in TNBC. Mitochondrial fission is prevalent in TNBC. These findings suggest that targeting the unique mitochondrial metabolism and dynamics in TNBC may offer a novel therapeutic strategy for patients with TNBC.
Synthesis of Single Nanometer-Sized Au Nanoparticles Coated with Silica Toward X-Ray Contrast Agent Peer-reviewed

Takeru Sakamoto, Noriko Yamauchi, Takumu Takase, Mone Kimura, Kohsuke Gonda, Yoshio Kobayashi

Solid State Phenomena　354　119-128　2023/12/20
Publisher: Trans Tech Publications, Ltd.
DOI： 10.4028/p-5loba0 　

eISSN： 1662-9779

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In this study, we proposed a method for fabricating diagnostic imaging nanoparticles composed of Au nanoparticles and silica shells (Au/SiO2). The proposed method consisted of two steps. The first step was the synthesis of Au nanoparticles. In sodium hydroxide (NaOH) solution, hydrogen tetrachloroaurate (III) trihydrate was reduced with tetrakis(hydroxymethyl)phosphonium chloride to synthesize Au nanoparticles with a diameter of 1.7 ± 0.3 nm. The Au nanoparticles were then coated with silica in the following step. The silica coating was achieved via a sol–gel reaction of tetraethyl orthosilicate in the presence of Au nanoparticles in water/ethanol dissolved in NaOH. The Au/SiO2 nanoparticles degraded faster in saline or phosphate-buffered saline than in water, and the X-ray imaging capability was retained.
Clinical Significance of ABCG2/BCRP Quantified by Fluorescent Nanoparticles in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy Peer-reviewed

Hiroshi Tada, Kohsuke Gonda, Narufumi Kitamura, Takanori Ishida

Cancers　15　(8)　2365-2365　2023/04/18
Publisher: MDPI AG
DOI： 10.3390/cancers15082365 　

eISSN： 2072-6694

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Breast cancer resistance protein (BCRP), also known as ATP-binding cassette transporter G2 (ABCG2), is associated with chemotherapy resistance. BCRP is also implicated in breast cancer stem cells, and is reported as a poor prognostic factor. However, the relationship of BCRP levels in breast cancer tissues with chemotherapy resistance and prognosis has not been clarified. We aimed to evaluate the correlation between BCRP expression and prognosis in breast cancer using immunohistochemistry with fluorescent phosphor-integrated dots (IHC-PIDs). A total of 37 breast cancer patients with residual cancer in the primary tumor and axillary lymph nodes were evaluated. BCRP levels in breast cancer tissue and metastatic lymph nodes were quantitatively detected after neoadjuvant chemotherapy (NAC). Among these 37 patients, 24 had corresponding core needle biopsies obtained before NAC. Biomarker assay with IHC-PIDs showed high accuracy for the quantitative assessment of BCRP with low expression. High BCRP expression in the primary tumor and metastatic lymph nodes after preoperative chemotherapy was associated with worse overall survival. In conclusion, high BCRP levels may be associated with poor prognosis in patients with breast cancer, having residual tumors within the primary tumor and lymph nodes after preoperative chemotherapy. These findings provide a basis for further appropriate adjuvant therapy in these patients.
Synthesis of Au nanoparticles coated with silica containing polyethylene glycol for extracorporeally dischargeable X-ray contrast agents Peer-reviewed

Takeru Sakamoto, Noriko Yamauchi, Shohei Tada, Takumu Takase, Mone Kimura, Chihiro Nishidate, Kohsuke Gonda, Yoshio Kobayashi

Journal of Nanoparticle Research　25　(4)　2023/03/27
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s11051-023-05726-1 　

ISSN： 1388-0764

eISSN： 1572-896X
Development of gold-coated magnetic nanoparticles as a theranostic agent for magnetic hyperthermia and CT imaging applications Peer-reviewed

Loi Tonthat, Mone Kimura, Tomoyuki Ogawa, Narufumi Kitamura, Yoshio Kobayashi, Kohsuke Gonda, Shin Yabukami

AIP Advances　13　(2)　025239-025239　2023/02/01
Publisher: AIP Publishing
DOI： 10.1063/9.0000592 　

eISSN： 2158-3226

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In this study, we aim to develop gold-coated Fe₃O₄ nanoparticles (Fe₃O₄@Au NPs) as theranostic agents for magnetic hyperthermia and CT imaging applications. The Fe₃O₄ NPs were synthesized via thermal decomposition method, and the gold was then deposited onto the surface of Fe₃O₄ NPs by reducing gold acetate at 190 °C. The average sizes of Fe₃O₄ and Fe₃O₄@Au NPs were 5.2 nm and 6.1 nm, respectively, which are effectively removed by the kidneys. The magnetization of Fe₃O₄@Au NPs (9.7 emu/g-Fe₃O₄) at 300 K was much smaller than that of Fe₃O₄ NPs (52.4 emu/g-Fe₃O₄). The heating efficiency of Fe₃O₄@Au NPs in water was sufficient to treat the tumor at 43–45 °C, and their high CT value of 851 HU was obtained. The synthesized ultrasmall Fe₃O₄@Au NPs showed great promise as a potential theranostic agent for magnetic hyperthermia and CT imaging applications.
X-ray irradiation negatively affects immune responses in the lymphatic network Peer-reviewed

Sawa Kanabuchi, Narufumi Kitamura, Mayumi Takano-Kasuya, Tomoya Inose, Chihiro Nishidate, Mizuki Yamanashi, Makoto Kudo, Tatsuki Ito, Naho Ito, Hiroshi Okamoto, Yusuke Taniyama, Yoshio Kobayashi, Takashi Kamei, Kohsuke Gonda

Microvascular Research　104511-104511　2023/02
Publisher: Elsevier BV
DOI： 10.1016/j.mvr.2023.104511 　

ISSN： 0026-2862
Development of methods for fabricating nanoparticles composed of magnetite, gold, and silica toward diagnostic imaging Peer-reviewed

Yukina Kobayashi, Michi Nagatsuka, Keisuke Akino, Noriko Yamauchi, Kouichi Nakashima, Tomoya Inose, Chihiro Nishidate, Keisuke Sato, Kohsuke Gonda, Yoshio Kobayashi

Colloids and Surfaces A: Physicochemical and Engineering Aspects　643　128773-128773　2022/06
Publisher: Elsevier BV
DOI： 10.1016/j.colsurfa.2022.128773 　

ISSN： 0927-7757
Fabrication and fluorescence imaging properties of indocyanine green-loaded poly(lactic-co-glycolic acid) nanoparticles Peer-reviewed

Mayu Tayama, Tomoya Inose, Noriko Yamauchi, Kouichi Nakashima, Masayuki Tokunaga, Chihiro Kato, Kohsuke Gonda, Yoshio Kobayashi

Colloid and Polymer Science　299　(8)　1271-1283　2021/08

DOI： 10.1007/s00396-021-04844-3 　

ISSN： 0303-402X

eISSN： 1435-1536
Fabrication of gold-immobilized quantum dots/silica core–shell nanoparticles and their multimodal imaging properties Peer-reviewed

M. Tayama, T. Inose, N. Yamauchi, K. Nakashima, M. Tokunaga, C. Kato, K. Gonda, Y. Kobayashi

Particulate Science and Technology　1-9　2021/06/08
Publisher: Informa UK Limited
DOI： 10.1080/02726351.2021.1934918 　

ISSN： 0272-6351

eISSN： 1548-0046
The anti-angiogenic agent lenvatinib induces tumor vessel normalization and enhances radiosensitivity in hepatocellular tumors. International-journal Peer-reviewed

Norikazu Une, Mayumi Takano-Kasuya, Narufumi Kitamura, Mineto Ohta, Tomoya Inose, Chihiro Kato, Ryuichi Nishimura, Hiroshi Tada, Shigehito Miyagi, Takanori Ishida, Michiaki Unno, Takashi Kamei, Kohsuke Gonda

Medical oncology (Northwood, London, England)　38　(6)　60-60　2021/04/21

DOI： 10.1007/s12032-021-01503-z 　

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The evaluation of angiogenesis inhibitors requires the analysis of the precise structure and function of tumor vessels. The anti-angiogenic agents lenvatinib and sorafenib are multi-target tyrosine kinase inhibitors that have been approved for the treatment of hepatocellular carcinoma (HCC). However, the different effects on tumor vasculature between lenvatinib and sorafenib are not well understood. In this study, we analyzed the effects of both drugs on vascular structure and function, including vascular normalization, and investigated whether the normalization had a positive effect on a combination therapy with the drugs and radiation using micro X-ray computed tomography with gold nanoparticles as a contrast agent, as well as immunohistochemical analysis and interstitial fluid pressure (IFP) measurement. In mice subcutaneously transplanted with mouse HCC cells, treatment with lenvatinib or sorafenib for 14 days inhibited tumor growth and reduced the tumor vessel volume density. However, analysis of integrated data on vessel density, rates of pericyte-covering and perfused vessels, tumor hypoxia, and IFP measured 4 days after drug treatment showed that treatment with 3 mg/kg of lenvatinib significantly reduced the microvessel density and normalized tumor vessels compared to treatment with 50 mg/kg of sorafenib. These results showed that lenvatinib induced vascular normalization and improved the intratumoral microenvironment in HCC tumors earlier and more effectively than sorafenib. Moreover, such changes increased the radiosensitivity of tumors and enhanced the effect of lenvatinib and radiation combination therapy, suggesting that this combination therapy is a powerful potential application against HCC.
Development of X-ray contrast agents using single nanometer-sized gold nanoparticles and lactoferrin complex and their application in vascular imaging. International-journal Peer-reviewed

Tomoya Inose, Narufumi Kitamura, Mayumi Takano-Kasuya, Masayuki Tokunaga, Norikazu Une, Chihiro Kato, Mayu Tayama, Yukina Kobayashi, Noriko Yamauchi, Daisuke Nagao, Takuji Aimiya, Naoko Furusawa, Yasushi Nakano, Yoshio Kobayashi, Kohsuke Gonda

Colloids and surfaces. B, Biointerfaces　203　111732-111732　2021/04/01

DOI： 10.1016/j.colsurfb.2021.111732 　

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The technology to accurately image the morphology of tumor vessels with X-ray contrast agents is important to clarify mechanisms underlying tumor progression and evaluate the efficacy of chemotherapy. However, in clinical practice, iodine-based contrast agents present problems such as short blood retention owing to a high clearance ability and insufficient X-ray absorption capacity when compared with other high atomic number elements. To resolve these issues, gold nanoparticles (AuNPs), with a high atomic number, have attracted a great deal of attention as contrast agents for angiography, and have been employed in small animal models. Herein, we developed novel contrast agents using AuNPs and captured changes in tumor vessel morphology with time using X-ray computed tomography (CT). First, glutathione-supported single nanometer-sized AuNPs (sAu/GSH) (diameter, 2.2 nm) were fabricated using tetrakis(hydroxymethyl)phosphonium chloride as a reducing agent. The sAu/GSH particles were intravenously injected into mice, remained in vessels for a few minutes, and were then excreted by the kidneys after 24 h, similar to the commercial contrast agent iopamidol. Next, the Au/GSH and lactoferrin (sAu/GSH-LF) (long axis size, 17.3 nm) complex was produced by adding lactoferrin to the sAu/GSH solution under the influence of a condensing agent. On intravenously administering sAu/GSH-LF to mice, the blood retention time was 1-3 h, which was considerably longer than that observed with iopamidol and sAu/GSH. Moreover, we succeeded in imaging morphological changes in identical tumor vessels for several days using X-ray CT with sAu/GSH-LF.
Development of composite nanoparticles composed of silica-coated nanorods and single nanometer-sized gold particles toward a novel X-ray contrast agent Peer-reviewed

Tomoya Inose, Takahiro Oikawa, Masayuki Tokunaga, Noriko Yamauchi, Kouichi Nakashima, Chihiro Kato, Keiichiro Hatoyama, Takashi Kamei, Kohsuke Gonda, Yoshio Kobayashi

Materials Science and Engineering: B　262　114716-114716　2020/12

DOI： 10.1016/j.mseb.2020.114716 　

ISSN： 0921-5107

eISSN： 1873-4944
Heterogeneous Drug Efficacy of an Antibody-Drug Conjugate Visualized Using Simultaneous Imaging of Its Delivery and Intracellular Damage in Living Tumor Tissues Peer-reviewed

Kohsuke Gonda, Hiroshi Negishi, Mayumi Takano-Kasuya, Narufumi Kitamura, Naoko Furusawa, Yasushi Nakano, Yoh Hamada, Masayuki Tokunaga, Hideo Higuchi, Hiroshi Tada, Takanori Ishida

Translational Oncology　13　(6)　100764-100764　2020/06
Publisher: Elsevier BV
DOI： 10.1016/j.tranon.2020.100764 　

ISSN： 1936-5233
A millisecond structured illumination microscope for super-resolution live cell imaging Peer-reviewed

Tomu Suzuki, Shinji Kajimoto, Narufumi Kitamura, Mayumi Takano-Kasuya, Naoko Furusawa, Yasushi Nakano, Hiroshi Fukumura, Kohsuke Gonda, Takakazu Nakabayashi

Applied Physics Express　13　(4)　045002 (4 page)　2020/03

DOI： 10.35848/1882-0786/ab7cef 　
Au nanoparticles coated with chitosan Peer-reviewed

Akitoshi Tokumasu, Tomoya Inose, Noriko Yamauchi, Kouichi Nakashima, Masayuki Tokunaga, Chihiro Kato, Keiichiro Hatoyama, Takashi Kamei, Kohsuke Gonda, Yoshio Kobayashi

COLLOID AND POLYMER SCIENCE　297　(7-8)　1143-1148　2019/08

DOI： 10.1007/s00396-019-04524-3 　

ISSN： 0303-402X

eISSN： 1435-1536
Fabrication and dual-modal imaging properties of quantum dot/silica core-shell particles with immobilized single-nanometer-sized gold nanoparticles Peer-reviewed

Mayu Tayama, Tomoya Inose, Noriko Yamauchi, Kouichi Nakashima, Masayuki Tokunaga, Chihiro Kato, Keiichiro Hatoyama, Takashi Kamei, Kohsuke Gonda, Yoshio Kobayashi

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS　574　162-170　2019/08

DOI： 10.1016/j.colsurfa.2019.04.055 　

ISSN： 0927-7757

eISSN： 1873-4359
Electron Microscopy Observations of the Au Nanorods and Au Nanorod/SiO₂ Nanocapsules Peer-reviewed

Kouichi NAKASHIMA, Tomoya INOSE, Takahiro OIKAWA, Masayuki TOKUNAGA, Keiichiro HATOYAMA, Masato KAKIHANA, Kohsuke GONDA, Yoshio KOBAYASHI

Journal of the Japan Society of Powder and Powder Metallurgy　66　(5)　210-214　2019/05/15
Publisher: Japan Society of Powder and Powder Metallurgy
DOI： 10.2497/jjspm.66.210 　

ISSN： 0532-8799

eISSN： 1880-9014
Automated Quantification of Extranuclear ERα using Phosphor-integrated Dots for Predicting Endocrine Therapy Resistance in HR+/HER2- Breast Cancer. International-journal Peer-reviewed

Zhaorong Guo, Hiroshi Tada, Narufumi Kitamura, Yoh Hamada, Minoru Miyashita, Narumi Harada-Shoji, Akiko Sato, Yohei Hamanaka, Kouki Tsuboi, Nobuhisa Harada, Mayumi Takano-Kasuya, Hisatake Okada, Yasushi Nakano, Noriaki Ohuchi, Shin-Ichi Hayashi, Takanori Ishida, Kohsuke Gonda

Cancers　11　(4)　2019/04/12

DOI： 10.3390/cancers11040526 　

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In addition to genomic signaling, Estrogen receptor alpha (ERα) is associated with cell proliferation and survival through extranuclear signaling contributing to endocrine therapy (ET) resistance. However, the relationship between extranuclear ERα and ET resistance has not been extensively studied. We sought to measure extranuclear ERα expression by immunohistochemistry using phosphor-integrated dots (IHC-PIDs) and to assess its predictive value for ET resistance. After quantitative detection of ERα by IHC-PIDs in vitro, we developed "the nearest-neighbor method" to calculate the extranuclear ERα. Furthermore, tissue sections from 65 patients with HR+/HER2- BC were examined by IHC-PIDs, and the total ERα, nuclear ERα, extranuclear ERα PIDs score, and ratio of extranuclear-to-nuclear ERα (ENR) were measured using the novel method. We demonstrate that quantification of ERα using IHC-PIDs exhibited strong correlations to real-time qRT-PCR (r2 = 0.94) and flow cytometry (r2 = 0.98). High ERα ENR was significantly associated with poor overall survival (p = 0.048) and disease-free survival (DFS) (p = 0.007). Multivariate analysis revealed that the ERα ENR was an independent prognostic factor for DFS [hazard ratio, 3.8; 95% CI, 1.4-11.8; p = 0.006]. Our automated measurement has high accuracy to localize and assess extranuclear ERα. A high ERα ENR in HR+/HER2- BC indicates decreased likelihood of benefiting from ET.
Influence of hydrolysis susceptibility and hydrophobicity of Sn-38 nano-prodrugs on their anticancer activity Peer-reviewed

Yoshitaka Koseki, Yoshikazu Ikuta, Liman Cong, Mayumi Takano-Kasuya, Hiroshi Tada, Mika Watanabe, Kohsuke Gonda, Takanori Ishida, Noriaki Ohuchi, Keita Tanita, Farsai Taemaitree, Anh Thi Ngoc Dao, Tsunenobu Onodera, Hidetoshi Oikawa, Hitoshi Kasai

Bulletin of the Chemical Society of Japan　92　(8)　1305-1313　2019

DOI： 10.1246/bcsj.20190088 　

ISSN： 0009-2673

eISSN： 1348-0634
Quantitative analyses of amount and localization of radiosensitizer gold nanoparticles interacting with cancer cells to optimize radiation therapy Peer-reviewed

Keiichiro Hatoyama, Narufumi Kitamura, Mayumi Takano-Kasuya, Masayuki Tokunaga, Takahiro Oikawa, Mineto Ohta, Yoh Hamada, Hiroshi Tada, Yoshio Kobayashi, Takashi Kamei, Kohsuke Gonda

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　508　(4)　1093-1100　2019/01

DOI： 10.1016/j.bbrc.2018.12.016 　

ISSN： 0006-291X

eISSN： 1090-2104
Synthesis on aggregation of colloidal solutions of ICG-active silica nanoparticles and their application in in-vivo fluorescence imaging Peer-reviewed

Yuta Shindo, Tomoya Inose, Yohsuke Kubota, Takahiro Oikawa, Masayuki Tokunaga, Takashi Kamei, Kohsuke Gonda, Yoshio Kobayashi

MATERIALS CHEMISTRY AND PHYSICS　220　201-207　2018/12

DOI： 10.1016/j.matchemphys.2018.08.073 　

ISSN： 0254-0584

eISSN： 1879-3312
The development of quantum dot/silica particles for fluorescence imaging and medical diagnostics

Yoshio Kobayashi, Kohsuke Gonda

Handbook of Sol-Gel Science and Technology: Processing, Characterization and Applications　3393-3430　2018/07/09
Publisher: Springer International Publishing
DOI： 10.1007/978-3-319-32101-1_126 　
Thermo-triggered Release of CRISPR-Cas9 System by Lipid-Encapsulated Gold Nanoparticles for Tumor Therapy Peer-reviewed

Peng Wang, Lingmin Zhang, Wenfu Zheng, Liman Cong, Zhaorong Guo, Yangzhouyun Xie, Le Wang, Rongbing Tang, Qiang Feng, Yoh Hamada, Kohsuke Gonda, Zhijian Hu, Xiaochun Wu, Xingyu Jiang

Angewandte Chemie - International Edition　57　(6)　1491-1496　2018/02/05

DOI： 10.1002/anie.201708689 　

ISSN： 1521-3773 1433-7851
Fabrication and dual imaging properties of quantum dot/silica core-shell particles immobilized with gold nanoparticles Peer-reviewed

Li Ting-ting, Inose Tomoya, Oikawa Takahiro, Tokunaga Masayuki, Hatoyama Keiichiro, Nakashima Kouichi, Kamei Takashi, Gonda Kohsuke, Kobayashi Yoshio

MATERIALS TECHNOLOGY　33　(11)　737-747　2018

DOI： 10.1080/10667857.2018.1502504 　

ISSN： 1066-7857

eISSN： 1753-5557
A comparison of five partial volume correction methods for Tau and Amyloid PET imaging with [F-18]THK5351 and [C-11]PIB Peer-reviewed

Miho Shidahara, Benjamin A. Thomas, Nobuyuki Okamura, Masanobu Ibaraki, Keisuke Matsubara, Senri Oyama, Yoichi Ishikawa, Shoichi Watanuki, Ren Iwata, Shozo Furumoto, Manabu Tashiro, Kazuhiko Yanai, Kohsuke Gonda, Hiroshi Watabe

ANNALS OF NUCLEAR MEDICINE　31　(7)　563-569　2017/08

DOI： 10.1007/s12149-017-1185-0 　

ISSN： 0914-7187

eISSN： 1864-6433
Prediction of the Clinical SUV Ratio in Amyloid PET Imaging Using a Biomathematic Modeling Approach Toward the Efficient Development of a Radioligand Peer-reviewed

Yuma Arakawa, YingHwey Nai, Miho Shidahara, Shozo Furumoto, Chie Seki, Nobuyuki Okamura, Manabu Tashiro, Yukitsuka Kudo, Kazuhiko Yanai, Kohsuke Gonda, Hiroshi Watabe

JOURNAL OF NUCLEAR MEDICINE　58　(8)　1285-1292　2017/08

DOI： 10.2967/jnumed.116.183566 　

ISSN： 0161-5505

eISSN： 1535-5667
Quantitative diagnostic imaging of cancer tissues by using phosphor-integrated dots with ultra-high brightness Peer-reviewed

Kohsuke Gonda, Mika Watanabe, Hiroshi Tada, Minoru Miyashita, Yayoi Takahashi-Aoyama, Takashi Kamei, Takanori Ishida, Shin Usami, Hisashi Hirakawa, Yoichiro Kakugawa, Yohei Hamanaka, Ryuichi Yoshida, Akihiko Furuta, Hisatake Okada, Hideki Goda, Hiroshi Negishi, Kensaku Takanashi, Masaru Takahashi, Yuichi Ozaki, Yuka Yoshihara, Yasushi Nakano, Noriaki Ohuchi

SCIENTIFIC REPORTS　7　2017/08

DOI： 10.1038/s41598-017-06534-z 　

ISSN： 2045-2322
Fabrication of silica-coated gold nanorods and investigation of their property of photothermal conversion Peer-reviewed

Tomoya Inose, Takahiro Oikawa, Kyosuke Shibuya, Masayuki Tokunaga, Keiichiro Hatoyama, Kouichi Nakashima, Takashi Kamei, Kohsuke Gonda, Yoshio Kobayashi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　484　(2)　318-322　2017/03

DOI： 10.1016/j.bbrc.2017.01.112 　

ISSN： 0006-291X

eISSN： 1090-2104
Fabrication of multilayered Au/silica/gadolinium compound core-shell particles and their imaging properties Peer-reviewed

Y. Kobayashi, Y. Shindo, T. Oikawa, M. Tokunaga, Y. Kubota, K. Gonda

MATERIALS SCIENCE AND TECHNOLOGY　33　(8)　963-970　2017

DOI： 10.1080/02670836.2016.1246120 　

ISSN： 0267-0836

eISSN： 1743-2847
Quantitative diagnosis of HER2 protein expressing breast cancer by single-particle quantum dot imaging Peer-reviewed

Minoru Miyashita, Kohsuke Gonda, Hiroshi Tada, Mika Watanabe, Narufumi Kitamura, Takashi Kamei, Hironobu Sasano, Takanori Ishida, Noriaki Ohuchi

CANCER MEDICINE　5　(10)　2813-2824　2016/10

DOI： 10.1002/cam4.898 　

ISSN： 2045-7634
Preparation of high-concentration colloidal solution of silica-coated gold nanoparticles and their application to X-ray imaging Peer-reviewed

Yoshio Kobayashi, Kyosuke Shibuya, Masayuki Tokunaga, Yohsuke Kubota, Takahiro Oikawa, Kohsuke Gonda

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY　78　(1)　82-90　2016/04

DOI： 10.1007/s10971-015-3921-z 　

ISSN： 0928-0707

eISSN： 1573-4846
Fabrication of gadolinium hydroxide nanoparticles using ion-exchange resin and their MRI property Peer-reviewed

Y. Kobayashi, H. Morimoto, T. Nakagawa, Y. Kubota, K. Gonda, N. Ohuchi

Journal of Asian Ceramic Societies　4　(1)　138-142　2016/03
Publisher: Informa UK Limited
DOI： 10.1016/j.jascer.2016.01.005 　

eISSN： 2187-0764
Fabrication of quantum dot/silica core-shell particles immobilizing Au nanoparticles and their dual imaging functions Peer-reviewed

Yoshio Kobayashi, Hiromu Matsudo, Ting-ting Li, Kyosuke Shibuya, Yohsuke Kubota, Takahiro Oikawa, Tomohiko Nakagawa, Kohsuke Gonda

APPLIED NANOSCIENCE　6　(3)　301-307　2016/03

DOI： 10.1007/s13204-015-0440-8 　

ISSN： 2190-5509

eISSN： 2190-5517
Preparation of a Colloid Solution of Au/Silica Core-Shell Nanoparticles Surface-Modified with Cellulose and Its X-ray Imaging Properties Peer-reviewed

Kobayashi, Y Nagasu R, Nakagawa T, Kubota Y, Gonda K, Ohuchi N

Journal of Nanomaterials & Molecular Nanotechnology　5　4　2016

DOI： 10.1080/02670836.2016.1246120 　
Performance evaluation of the small-animal PET scanner ClairvivoPET using NEMA NU 4-2008 Standards Peer-reviewed

K. Sato, M. Shidahara, H. Watabe, S. Watanuki, Y. Ishikawa, Y. Arakawa, Y. H. Nai, S. Furumoto, M. Tashiro, T. Shoji, K. Yanai, K. Gonda

PHYSICS IN MEDICINE AND BIOLOGY　61　(2)　696-711　2016/01

DOI： 10.1088/0031-9155/61/2/696 　

ISSN： 0031-9155

eISSN： 1361-6560
Stabilization of silica-coated silver iodide nanoparticles by ethanol-washing Peer-reviewed

Yoshio Kobayashi, Tetsuya Ayame, Kyosuke Shibuya, Tomohiko Nakagawa, Yohsuke Kubota, Kohsuke Gonda, Noriaki Ohuchi

PIGMENT & RESIN TECHNOLOGY　45　(2)　99-105　2016

DOI： 10.1108/PRT-10-2014-0083 　

ISSN： 0369-9420

eISSN： 1758-6941
X-ray computed tomography imaging of a tumor with high sensitivity using gold nanoparticles conjugated to a cancer-specific antibody via polyethylene glycol chains on their surface Peer-reviewed

Tomohiko Nakagawa, Kohsuke Gonda, Takashi Kamei, Liman Cong, Yoh Hamada, Narufumi Kitamura, Hiroshi Tada, Takanori Ishida, Takuji Aimiya, Naoko Furusawa, Yasushi Nakano, Noriaki Ohuchi

SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS　17　(1)　387-397　2016

DOI： 10.1080/14686996.2016.1194167 　

ISSN： 1468-6996

eISSN： 1878-5514
Cytotoxicity of Pure Nanodrugs of SN-38 and Podophyllotoxin Dimers in Human Cancer HepG2, KPL-4, and MCF-7 Cells Peer-reviewed

Yoshitaka Koseki, Yoshikazu Ikuta, Tatsuya Murakami, Tsunenobu Onodera, Hidetoshi Oikawa, Liman Cong, Hiroshi Tada, Kohsuke Gonda, Noriaki Ohuchi, Hitoshi Kasai

MOLECULAR CRYSTALS AND LIQUID CRYSTALS　622　(1)　1-5　2015/11

DOI： 10.1080/15421406.2015.1096483 　

ISSN： 1542-1406

eISSN： 1563-5287
Predictive diagnosis of the risk of breast cancer recurrence after surgery by single-particle quantum dot imaging. International-journal

Kohsuke Gonda, Minoru Miyashita, Hideo Higuchi, Hiroshi Tada, Tomonobu M Watanabe, Mika Watanabe, Takanori Ishida, Noriaki Ohuchi

Scientific reports　5　14322-14322　2015/09/22

DOI： 10.1038/srep14322 　

More details Close

In breast cancer, the prognosis of human epidermal growth factor receptor 2 (HER2)-positive patients (20-25%) has been dramatically improved by the clinical application of the anti-HER2 antibody drugs trastuzumab and pertuzumab. However, the clinical outcomes of HER2-negative cases with a poor prognosis have not improved, and novel therapeutic antibody drugs or diagnostic molecular markers of prognosis are urgently needed. Here, we targeted protease-activated receptor 1 (PAR1) as a new biomarker for HER2-negative patients. The developed anti-PAR1 antibody inhibited PAR1 activation by matrix metalloprotease 1 and thereby prevented cancer-cell migration and invasion. To estimate PAR1 expression levels in HER2-negative patient tissues using the antibody, user-friendly immunohistochemistry with fluorescence nanoparticles or quantum dots (QDs) was developed. Previously, immunohistochemistry with QDs was affected by tissue autofluorescence, making quantitative measurement extremely difficult. We significantly improved the quantitative sensitivity of immunohistochemistry with QDs by using an autofluorescence-subtracted image and single-QD imaging. The immunohistochemistry showed that PAR1 expression was strongly correlated with relapse-free survival time in HER2-negative breast cancer patients. Therefore, the developed anti-PAR1 antibody is a strong candidate for use as an anticancer drug and a prognostic biomarker for HER2-negative patients.
Quantitative kinetic analysis of PET amyloid imaging agents [C-11]BF227 and [F-18]FACT in human brain Peer-reviewed

Miho Shidahara, Hiroshi Watabe, Manabu Tashiro, Nobuyuki Okamura, Shozo Furumoto, Shoichi Watanuki, Katsutoshi Furukawa, Yuma Arakawa, Yoshihito Funaki, Ren Iwata, Kohsuke Gonda, Yukitsuka Kudo, Hiroyuki Arai, Kiichi Ishiwata, Kazuhiko Yanai

NUCLEAR MEDICINE AND BIOLOGY　42　(9)　734-744　2015/09

DOI： 10.1016/j.nucmedbio.2015.05.001 　

ISSN： 0969-8051

eISSN： 1872-9614
Alternating current magnetic susceptibility and heat dissipation by Mn1-xZnxFe2O4 nanoparticles for hyperthermia treatment Peer-reviewed

T. Kondo, K. Mori, M. Hachisu, T. Yamazaki, D. Okamoto, M. Watanabe, K. Gonda, H. Tada, Y. Hamada, M. Takano, N. Ohuchi, Y. Ichiyanagi

JOURNAL OF APPLIED PHYSICS　117　(17)　17D157　2015/05

DOI： 10.1063/1.4919327 　

ISSN： 0021-8979

eISSN： 1089-7550
Clinical significance of subtype classification in metastatic lymph nodes of breast cancer patients undergoing neoadjuvant chemotherapy Peer-reviewed

Noriko Nemoto, Yukiko Shibahara, Hiroshi Tada, Keiko Uchida, Keely M. McNamara, Monica S. M. Chan, Mika Watanabe, Kentaro Tamaki, Minoru Miyashita, Yasuhiro Miki, Kohsuke Gonda, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano

INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS　30　(2)　E174-E183　2015/04

DOI： 10.5301/jbm.5000128 　

ISSN： 0393-6155

eISSN： 1724-6008
Direct immobilization of gadolinium complex on silica particles and their MRI properties Peer-reviewed

Yoshio Kobayashi, Kyosuke Shibuya, Tomohiko Nakagawa, Yohsuke Kubota, Kohsuke Gonda, Noriaki Ohuchi

e-Journal of Surface Science and Nanotechnology　13　42-46　2015/02/21

DOI： 10.1380/ejssnt.2015.42 　

ISSN： 1348-0391
Preparation of Silica-Coated Quantum Dot Nanoparticle Colloid Solutions and Their Application in in-vivo Fluorescence Imaging Peer-reviewed

Yoshio Kobayashi, Hiromu Matsudo, Yohsuke Kubota, Tomohiko Nakagawa, Kohsuke Gonda, Noriaki Ohuchi

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN　48　(2)　112-117　2015/02

DOI： 10.1252/jcej.14we218 　

ISSN： 0021-9592
Highly Sensitive Imaging of Cancer with Functional Nanoparticles Peer-reviewed

Kohsuke Gonda, Yoh Hamada, Narufumi Kitamura, Hiroshi Tada, Minoru Miyashita, Takashi Kamei, Takanori Ishida, Noriaki Ohuchi

JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY　28　(6)　731-736　2015

DOI： 10.2494/photopolymer.28.731 　

ISSN： 0914-9244
Preparation of Au/silica/poly(ethylene glycol) nanoparticle colloid solution and its use in x-ray imaging process Peer-reviewed

Kobayashi Yoshio, Nagasu Ryoko, Nakagawa Tomohiko, Kubota Yohsuke, Gonda Kohsuke, Ohuchi Noriaki

NANOCOMPOSITES　1　(2)　83-88　2015

DOI： 10.1179/2055033215Y.0000000003 　

ISSN： 2055-0324
Synthesis of a colloid solution of silica-coated gold nanoparticles for X-ray imaging applications Peer-reviewed

Yoshio Kobayashi, Ryoko Nagasu, Kyosuke Shibuya, Tomohiko Nakagawa, Yohsuke Kubota, Kohsuke Gonda, Noriaki Ohuchi

JOURNAL OF NANOPARTICLE RESEARCH　16　(8)　2014/07

DOI： 10.1007/s11051-014-2551-7 　

ISSN： 1388-0764

eISSN： 1572-896X
X-ray imaging technique using colloid solution of Au/silica/poly(ethylene glycol) nanoparticles Peer-reviewed

Y. Kobayashi, H. Inose, R. Nagasu, T. Nakagawa, Y. Kubota, K. Gonda, N. Ohuchi

Materials Research Innovations　17　(7)　507-514　2013/11

DOI： 10.1179/1433075X13Y.0000000100 　

ISSN： 1432-8917 1433-075X
Single Quantum Dot Tracking Reveals that an Individual Multivalent HIV-1 Tat Protein Transduction Domain Can Activate Machinery for Lateral Transport and Endocytosis Peer-reviewed

Yasuhiro Suzuki, Chandra Nath Roy, Warunya Promjunyakul, Hiroyasu Hatakeyama, Kohsuke Gonda, Junji Imamura, Biju Vasudevanpillai, Noriaki Ohuchi, Makoto Kanzaki, Hideo Higuchi, Mitsuo Kaku

MOLECULAR AND CELLULAR BIOLOGY　33　(15)　3036-3049　2013/08

DOI： 10.1128/MCB.01717-12 　

ISSN： 0270-7306

eISSN： 1098-5549
In-vivo fluorescence imaging technique using colloid solution of multiple quantum dots/silica/poly(ethylene glycol) nanoparticles Peer-reviewed

Yoshio Kobayashi, Hiromu Matsudo, Tomohiko Nakagawa, Yohsuke Kubota, Kohsuke Gonda, Noriaki Ohuchi

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY　66　(1)　31-37　2013/04

DOI： 10.1007/s10971-013-2962-4 　

ISSN： 0928-0707

eISSN： 1573-4846
Preparation of silica-coated gadolinium compound particle colloid solution and its application in imaging Peer-reviewed

Kobayashi Y, Morimoto H, Nakagawa T, Gonda K, Ohuchi N

Advanced in Nano Research　1　(3)　159-169　2013

DOI： 10.1252/jcej.14we218 　
X-ray imaging technique using colloid solution of Au/silica core-shell nanoparticles Peer-reviewed

Kobayashi Y, Inose H, Nakagawa T, Kubota Y, Gonda K, Ohuchi N

Journal of Nanostructure in Chemistry　3　62　2013
Preparation of AgI/silica/poly(ethylene glycol) nanoparticle colloid solution and X-ray imaging using it Peer-reviewed

Kobayashi Y, Ayame T, Nakagawa T, Kubota Y, Gonda K, Ohuchi N

ISRN Nanomaterials　670402　1-5　2013

DOI： 10.1179/1433075X13Y.0000000100 　
Fabrication of multilayered Au/silica/gadolinium compound/silica core-shell particles Peer-reviewed

Kobayashi Y, Inose H, Nakagawa T, Kubota Y, Gonda K, Ohuchi N

Materials Focus　2　369-373　2013

DOI： 10.1080/02670836.2016.1246120 　
Fabrication of hollow particles composed of silica containing gadolinium compound and magnetic resonance imaging using them Peer-reviewed

Kobayashi Y, Morimoto H, Nakagawa T, Kubota Y, Gonda K, Ohuchi N

Journal of Nanostructure in Chemistry　3　11　2013
Preparation of Gd Complex-Immobilized Silica Particles and Their Application to MRI Peer-reviewed

Kobayashi Y, Morimoto H, Nakagawa T, Kubota Y, Gonda K, Ohuchi N

ISRN Nanotechnology　908614　1-6　2013
Development of Silica-Coated Silver Iodide Nanoparticles and Their Biodistribution Peer-reviewed

Yu Sakurai, Hiroshi Tada, Kohsuke Gonda, Motohiro Takeda, Liman Cong, Masakazu Amari, Yoshio Kobayashi, Mika Watanabe, Takanori Ishida, Noriaki Ohuchi

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE　228　(4)　317-323　2012/12

DOI： 10.1620/tjem.228.317 　

ISSN： 0040-8727

eISSN： 1349-3329
Multilayered, core/shell nanoprobes based on magnetic ferric oxide particles and quantum dots for multimodality imaging of breast cancer tumors Peer-reviewed

Qiang Ma, Yuko Nakane, Yuki Mori, Miyuki Hasegawa, Yoshichika Yoshioka, Tomonobu M. Watanabe, Kohsuke Gonda, Noriaki Ohuchi, Takashi Jin

BIOMATERIALS　33　(33)　8486-8494　2012/11

DOI： 10.1016/j.biomaterials.2012.07.051 　

ISSN： 0142-9612
Mouse respiratory cilia with the asymmetric axonemal structure on sparsely distributed ciliary cells can generate overall directional flow Peer-reviewed

Hironori Ueno, Takuji Ishikawa, Khanh Huy Bui, Kohsuke Gonda, Takashi Ishikawa, Takami Yamaguchi

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE　8　(7)　1081-1087　2012/10

DOI： 10.1016/j.nano.2012.01.004 　

ISSN： 1549-9634
Development of a quantitative diagnostic method of estrogen receptor expression levels by immunohistochemistry using organic fluorescent material-assembled nanoparticles Peer-reviewed

Kohsuke Gonda, Minoru Miyashita, Mika Watanabe, Yayoi Takahashi, Hideki Goda, Hisatake Okada, Yasushi Nakano, Hiroshi Tada, Masakazu Amari, Noriaki Ohuchi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　426　(3)　409-414　2012/09

DOI： 10.1016/j.bbrc.2012.08.105 　

ISSN： 0006-291X
Synthesis of High Concentration Colloid Solution of Silica-Coated AgI Nanoparticles Peer-reviewed

Yoshio Kobayashi, Marie Minato, Kazuaki Ihara, Tomohiko Nakagawa, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi, Atsuo Kasuya

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY　12　(8)　6741-6745　2012/08

DOI： 10.1166/jnn.2012.4531 　

ISSN： 1533-4880
Synthesis of Au-silica core-shell particles by sol-gel process Peer-reviewed

Y. Kobayashi, H. Inose, T. Nakagawa, K. Gonda, M. Takeda, N. Ohuchi, A. Kasuya

SURFACE ENGINEERING　28　(2)　129-133　2012/03

DOI： 10.1179/1743294411Y.0000000069 　

ISSN： 0267-0844
Fabrication and fluorescence properties of multilayered core-shell particles composed of quantum dot, gadolinium compound, and silica Peer-reviewed

Yoshio Kobayashi, Takuya Nozawa, Tomohiko Nakagawa, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi

JOURNAL OF MATERIALS SCIENCE　47　(4)　1852-1859　2012/02

DOI： 10.1007/s10853-011-5972-z 　

ISSN： 0022-2461

eISSN： 1573-4803
Immobilization of magnetite on core-shell particles Peer-reviewed

Kobayashi Y, Fujisaku T, Mine E, Takezawa T, Nakagawa T, Gonda K, Takeda M, Ohuchi N

International Journal on Intelligent Electronic Systems　6　40-45　2012
X-ray imaging technique using colloid solution of AgI/silica/poly(ethylene glycol) nanoparticles Peer-reviewed

Kobayashi Y, Ayame T, Nakagawa T, Gonda K, Ohuchi N

Materials Focus　1　127-130　2012

DOI： 10.1007/s10971-013-2962-4 　
X-ray imaging of newly-developed gadolinium compound/silica core-shell particles Peer-reviewed

Hikaru Morimoto, Marie Minato, Tomohiko Nakagawa, Masahide Sato, Yoshio Kobayashi, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi, Noboru Suzuki

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY　59　(3)　650-657　2011/09

DOI： 10.1007/s10971-011-2540-6 　

ISSN： 0928-0707
In vivo imaging of the molecular distribution of the VEGF receptor during angiogenesis in a mouse model of ischemia Peer-reviewed

Yoh Hamada, Kohsuke Gonda, Motohiro Takeda, Akira Sato, Mika Watanabe, Tomoyuki Yambe, Susumu Satomi, Noriaki Ohuchi

BLOOD　118　(13)　E93-E100　2011/09

DOI： 10.1182/blood-2010-12-322842 　

ISSN： 0006-4971
Control of shell thickness in silica-coating of Au nanoparticles and their X-ray imaging properties Peer-reviewed

Yoshio Kobayashi, Hiromitsu Inose, Tomohiko Nakagawa, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi, Atsuo Kasuya

JOURNAL OF COLLOID AND INTERFACE SCIENCE　358　(2)　329-333　2011/06

DOI： 10.1016/j.jcis.2011.01.058 　

ISSN： 0021-9797
Preparation of silica-coated AgI nanoparticles by an amine-free process and their X-ray imaging properties Peer-reviewed

Tetsuya Ayame, Yoshio Kobayashi, Tomohiko Nakagawa, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi

JOURNAL OF THE CERAMIC SOCIETY OF JAPAN　119　(1390)　397-401　2011/06

DOI： 10.2109/jcersj2.119.397 　

ISSN： 1882-0743

eISSN： 1348-6535
Single Particle Tracking Confirms That Multivalent Tat Protein Transduction Domain-induced Heparan Sulfate Proteoglycan Cross-linkage Activates Rac1 for Internalization Peer-reviewed

Junji Imamura, Yasuhiro Suzuki, Kohsuke Gonda, Chandra Nath Roy, Hiroyuki Gatanaga, Noriaki Ohuchi, Hideo Higuchi

JOURNAL OF BIOLOGICAL CHEMISTRY　286　(12)　10581-10592　2011/03

DOI： 10.1074/jbc.M110.187450 　

ISSN： 0021-9258
Uniform Silica Coated Fluorescent Nanoparticles: Synthetic Method, Improved Light Stability and Application to Visualize Lymph Network Tracer Peer-reviewed

Liman Cong, Motohiro Takeda, Yohei Hamanaka, Kohsuke Gonda, Mika Watanabe, Masutaka Kumasaka, Yoshio Kobayashi, Masaki Kobayashi, Noriaki Ohuchi

PLOS ONE　5　(10)　e13167-e13167　2010/10

DOI： 10.1371/journal.pone.0013167 　

ISSN： 1932-6203
Direct coating of quantum dots with silica shell Peer-reviewed

Yoshio Kobayashi, Takuya Nozawa, Tomohiko Nakagawa, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi, Atsuo Kasuya

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY　55　(1)　79-85　2010/07

DOI： 10.1007/s10971-010-2218-5 　

ISSN： 0928-0707
Myosin-X Induces Filopodia by Multiple Elongation Mechanism Peer-reviewed

Tomonobu M. Watanabe, Hiroshi Tokuo, Kohsuke Gonda, Hideo Higuchi, Mitsuo Ikebe

JOURNAL OF BIOLOGICAL CHEMISTRY　285　(25)　19605-19614　2010/06

DOI： 10.1074/jbc.M109.093864 　

ISSN： 0021-9258
Nano-imaging of the lymph network structure with quantum dots Peer-reviewed

Makoto Hikage, Kohsuke Gonda, Motohiro Takeda, Takashi Kamei, Masaki Kobayashi, Masutaka Kumasaka, Mika Watanabe, Susumu Satomi, Noriaki Ohuchi

NANOTECHNOLOGY　21　(18)　185103-185103　2010/05

DOI： 10.1088/0957-4484/21/18/185103 　

ISSN： 0957-4484
In Vivo Nano-imaging of Membrane Dynamics in Metastatic Tumor Cells Using Quantum Dots Peer-reviewed

Kohsuke Gonda, Tomonobu M. Watanabe, Noriaki Ohuchi, Hideo Higuchi

JOURNAL OF BIOLOGICAL CHEMISTRY　285　(4)　2750-2757　2010/01

DOI： 10.1074/jbc.M109.075374 　

ISSN： 0021-9258

eISSN： 1083-351X
Intracellular imaging of targeted proteins labeled with quantum dots Peer-reviewed

Jungwoo Ybo, Taketoshi Kambara, Kohsuke Gonda, Hideo Higuchi

EXPERIMENTAL CELL RESEARCH　314　(19)　3563-3569　2008/11

DOI： 10.1016/j.yexcr.2008.09.014 　

ISSN： 0014-4827
Centrin controls the activity of the ciliary reversal-coupled volt-age-gated Ca2+ channels Ca2+-dependently Peer-reviewed

Kohsuke Gonda, Kazunori Oami, Mihoko Takahashi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　362　(1)　170-176　2007/10

DOI： 10.1016/j.bbrc.2007.07.173 　

ISSN： 0006-291X
Three-dimensional nanometry of vesicle transport in living cells using dual-focus imaging optics Peer-reviewed

Tornonobu M. Watanabe, Takashi Sato, Kohsuke Gonda, Hideo Higuchi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　359　(1)　1-7　2007/07

DOI： 10.1016/j.bbrc.2007.04.168 　

ISSN： 0006-291X
2P243 Imaging of membrane protrusion of cellular migration in tumor using quantum dots(39. Cell motility,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Gonda Kohsuke, Watanabe Tomonobu M, Higuchi Hideo

Seibutsu Butsuri　46　(2)　S356　2006
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.46.S356_3 　
Centrin is essential for the activity of the ciliary reversal-coupled voltage-gated Ca2+ channels Peer-reviewed

K Gonda, A Yoshida, K Oami, M Takahashi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　323　(3)　891-897　2004/10

DOI： 10.1016/j.bbrc.2004.08.172 　

ISSN： 0006-291X
Identification of elongation factor-1 alpha as a Ca2+/calmodulin-binding protein in Tetrahymena cilia Peer-reviewed

H Ueno, K Gonda, T Takeda, O Numata

CELL MOTILITY AND THE CYTOSKELETON　55　(1)　51-60　2003/05

DOI： 10.1002/cm.10111 　

ISSN： 0886-1544
p85 binds to G-actin in a Ca2+/calmodulin-dependent manner, thus regulating the initiation of cytokinesis in Tetrahymena Peer-reviewed

K Gonda, O Numata

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　292　(4)　1098-1103　2002/04

DOI： 10.1006/bbrc.2002.6777 　

ISSN： 0006-291X
Calmodulin and Ca2+/calmodulin-binding proteins are involved in Tetrahymena thermophila phagocytosis Peer-reviewed

K Gonda, M Komatsu, O Numata

CELL STRUCTURE AND FUNCTION　25　(4)　243-251　2000/08

DOI： 10.1247/csf.25.243 　

ISSN： 0386-7196

eISSN： 1347-3700
Cloning and sequencing of the gene for a Tetrahymena fimbrin-like protein Peer-reviewed

A Watanabe, Yonemura, I, K Gonda, O Numata

JOURNAL OF BIOCHEMISTRY　127　(1)　85-94　2000/01

DOI： 10.1093/oxfordjournals.jbchem.a022587 　

ISSN： 0021-924X
Tetrahymena elongation factor-1 alpha is localized with calmodulin in the division furrow Peer-reviewed

O Numata, Y Kurasawa, K Gonda, Y Watanabe

JOURNAL OF BIOCHEMISTRY　127　(1)　51-56　2000/01

DOI： 10.1093/oxfordjournals.jbchem.a022583 　

ISSN： 0021-924X

eISSN： 1756-2651
Molecular cloning of the gene for p85 that regulates the initiation of cytokinesis in Tetrahymena Peer-reviewed

K Gonda, K Nishibori, H Ohba, A Watanabe, O Numata

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　264　(1)　112-118　1999/10

DOI： 10.1006/bbrc.1999.1354 　

ISSN： 0006-291X
Ca2+/calmodulin and p85 cooperatively regulate an initiation of cytokinesis in Tetrahymena Peer-reviewed

Kohsuke Gonda, Mariko Katoh, Kazuko Hanyu, Yoshio Watanabe, Osamu Numata

Journal of Cell Science　112　(21)　3619-3626　1999

ISSN： 0021-9533

Show all ︎Show first 5

Misc. 37

Visualization of Biological Information and Quantitative Evaluation of Pathological Conditions Using Functional Nanoparticles Invited

北村成史, 多田寛, 高野真由美, 小林芳男, 石田孝宣, 亀井尚, 権田幸祐

化学工業　72　(4)　2021

ISSN： 0451-2014
Supporting Technology for Development of Antibody Drug and Diagnostic Method to Predict Response to the Drug, by Using Fluorescence Imaging Invited

権田幸祐, 根岸洋, 高野真由美, 北村成史, 古澤直子, 中野寧, 多田寛, 石田孝宣

癌と化学療法　48　(2)　2021

ISSN： 0385-0684
超高輝度蛍光ナノ粒子を用いたイメージングによるがん抗体医薬の薬効予測診断 Invited

権田幸祐, 中野寧, 多田寛, 石田孝宣

化学工業　(5月号)　42-47　2019/05
蛍光ナノ粒子を用いたがん組織の高感度定量イメージング

権田幸祐, 宮下穣, 多田寛, 中野寧

がん分子標的治療　16　(3)　287-292　2018/10
Publisher: (株)メディカルレビュー社
ISSN： 1347-6955
蛍光ナノ粒子を用いたがん組織の高感度定量イメージング Invited

権田幸祐

がん分子標的治療　16　(3)　59-64　2018/09
【in vivoイメージングとセラノスティクスの新展開】がん病態の定量的ナノバイオイメージング Invited

Drug Delivery System　33　(3)　179-189　2018/07
Publisher: 日本DDS学会
ISSN： 0913-5006
Quantitative nano-bio-imaging of cancer disease state

Kohsuke Gonda, Naoko Furusawa, Yasushi Nakano, Yoshio Kobayashi

Drug Delivery System　33　(3)　179-189　2018
Publisher: Japan Society of Drug Delivery System
DOI： 10.2745/dds.33.179 　

ISSN： 1881-2732 0913-5006
1粒子蛍光イメージングによる高精度がん組織診断法 Invited

ファルマシア　54　(1)　36-40　2018/01
Publisher: (公社)日本薬学会
DOI： 10.14894/faruawpsj.54.1_36 　

ISSN： 0014-8601
【乳癌学-最新の診断と治療-】乳癌最新の基礎研究動向ナノテクノロジーの乳癌診療への応用

多田寛, 郭照蓉, 宮下穣, 権田幸祐, 大内憲明

日本臨床　75　(増刊3 乳癌学)　181-185　2017/04
Publisher: (株)日本臨床社
ISSN： 0047-1852
【『診る』バイオマテリアル】機能性ナノ粒子でがんの病態を診る Invited

バイオマテリアル-生体材料-　34　(3)　224-229　2016/07
Publisher: 日本バイオマテリアル学会
ISSN： 1347-7080
【がんの先制医療のための画像診断技術】蛍光ナノバイオによるがん病態イメージング Invited

GONDA Kohsuke, OHUCHI Noriaki

MEDICAL IMAGING TECHNOLOGY　34　(2)　61-67　2016/03
Publisher: 日本医用画像工学会
DOI： 10.11409/mit.34.61 　

ISSN： 0288-450X
生体数学モデルを用いたアミロイドPETイメージングにおける臨床SUVR予測

荒川悠真, 志田原美保, Nai Ying Hwey, 古本祥三, 関千江, 岡村信行, 田代学, 工藤幸司, 谷内一彦, 権田幸祐, 渡部浩司

核医学　52　(3)　260-260　2015/09
Publisher: (一社)日本核医学会
ISSN： 0022-7854
ナノメディシンが拓く医学・医療研究の新領域 Invited

東北医学雑誌　126　(2)　153-158　2014/12
Publisher: 東北医学会
ISSN： 0040-8700
In Vivo Dual Imaging of Lymph Node Using X-ray CT and Fluorescence

12　(2)　69-72　2014/03
Publisher: ナノ学会
ISSN： 1347-8028
X線CTイメージングへのナノ粒子造影剤の応用 Invited

東北大学医学部保健学科紀要　22　(2)　61-66　2013/07
Publisher: 東北大学医学部保健学科
ISSN： 1348-8899
Imaging of Sentinel Lymph Node in an Animal Model with Silica-Coated Fluorescent Nanoparticles

Sou R, Gonda K, Tada H, Hamanaka Y, Amari M, Ishida T, Ohuchi N

Tohoku Igaku Zasshi　125　171-173　2013
CT imaging of tumor with Au nanoparticle Invited

ナノ学会会報 = The bulletin of the Society of Nano Science and Technology　11　(1)　27-30　2012/10
Publisher: ナノ学会
ISSN： 1347-8028
In vivo Molecular Imaging of the Distribution of VEGF Receptor in Angiogenesis

10　(1)　35-39　2011/10
Publisher: ナノ学会
ISSN： 1347-8028
【癌と生体イメージング】ナノイメージングで探る癌転移の仕組み (Surgery Frontier) Invited

Surgery Frontier　18　(1)　50-57　2011/03
がん転移の生体ナノイメージング (生物物理) Invited

権田幸祐, 渡邉朋信, 大内憲明, 樋口秀男

生物物理　51　(2)　82-83　2011/03
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.51.082 　

ISSN： 0582-4052
バイオ1分子メージングとナノ計測 Invited

樋口秀男, 茅元司, 権田幸祐

Molecular electronics and bioelectronics　21　(4)　247-252　2010/11
Micro-structural imaging of the lymph networks with quantum dots Invited

The bulletin of the Society of Nano Science and Technology　9　(1)　13-17　2010/10
Publisher: ナノ学会
ISSN： 1347-8028
ナノサイズ抗がん剤の創薬へ応用可能なin vivoナノイメージング法の開発 (東北医学雑誌) Invited

権田幸祐

東北医学雑誌　122　(1)　113-115　2010/06
ナノ粒子を用いた内視鏡手術下蛍光センチネルリンパ生検 (東北医学雑誌) Invited

日景允, 権田幸祐, 武田元博, 亀井尚, 大内憲明

東北医学雑誌　122　(1)　91-94　2010/06
【ナノテクノロジーの外科領域への応用】蛍光ナノトレーサーを用いたセンチネルリンパ節生検 (Surgery Frontier) Invited

武田元博, 日景允, 権田幸祐, 叢莉蔓, 大内憲明

Surgery Frontier　17　(2)　131-134　2010/06
ナノ医療のためのin vivoナノイメージング (現代化学) Invited

権田幸祐, 武田元博, 大内憲明

現代化学　(464)　49-54　2009/11
Publisher: 東京化学同人
ISSN： 0386-961X
【ナノ粒子メディスン】ナノテクノロジーと乳腺外科 (医学のあゆみ) Invited

武田元博, 権田幸祐, 大内憲明

医学のあゆみ　230　(8)　507-511　2009/08
機能性ナノ粒子を用いたDDS並びに腫瘍細胞動態のin vivoイメージング Invited

大内憲明, 武田元博, 権田幸祐

ナノ医工学年報　2　(1)　183-190　2009/03
Publisher: 東北大学グローバルCOEプログラム「新世紀世界の成長焦点に築くナノ医工学拠点」
ISSN： 1882-4692
Bio-imaging by functional nano-particles of nano to macro scale

M. Takeda, H. Tada, M. Kawai, Y. Sakurai, H. Higuchi, K. Gonda, T. Ishida, N. Ohuchi

IFMBE Proceedings　23　2272-2274　2009

DOI： 10.1007/978-3-540-92841-6_570 　

ISSN： 1680-0737
分子イメージング In vivoナノイメージングで解き明かすがん転移とDDSの仕組み (Drug Delivery System)

権田幸祐, 渡邉朋信, 河合賢朗, 武田元博, 樋口秀男, 大内憲明

Drug Delivery System　24　(3)　276-276　2009
【がん分子イメージングの新展開】がん分子イメージングの新展開 (癌と化学療法) Invited

武田元博, 権田幸祐, 樋口秀男, 大内憲明

癌と化学療法　35　(8)　1277-1280　2008/08
[In vivo single molecular fluorescence imaging for analysis of pharmacokinetics]

Takeda Motohiro, Gonda Kohsuke, Higuchi Hideo, Ohuchi Noriaki

Gan To Kagaku Ryoho　35　(8)　1277-1280　2008
医学領域におけるナノ粒子展開をめぐる話題機能性ナノ粒子による生体イメージングの臨床検査への応用 (臨床病理)

武田元博, 権田幸祐, 桜井遊, 河合賢朗, 石田孝宣, 大内憲明

臨床病理　56　(補冊)　60-60　2008
生体超微細1分子可視化技術によるナノDDSとがん標的治療 (新しい医療機器研究)

大内憲明, 渡邉朋信, 武田元博, 石田直理雄, 川添良幸, 粕谷厚生, 水関博志, 小林正樹, 小林芳男, 名嘉尚, 亀井尚, 甘利正和, 権田幸祐, 仲田栄子, 多田寛, 魚森昌彦

新しい医療機器研究　12　21-22　2007
Publisher: (財)医療機器センター情報サービス部
ISSN： 0918-435X
Determination of division plane and organization of contractile ring in Tetrahymena Invited Peer-reviewed

Osamu Numata, Kohsuke Gonda

Cell Structure and Function　26　(6)　593-601　2001

DOI： 10.1247/csf.26.593 　

ISSN： 0386-7196
Cytokinesis in Tetrahymena: Determination of division plane and organization of contractile ring Invited Peer-reviewed

O Numata, K Gonda, A Watanabe, Y Kurasawa

MICROSCOPY RESEARCH AND TECHNIQUE　49　(2)　127-135　2000/04

ISSN： 1059-910X

eISSN： 1097-0029
Macronuclear division and cytokinesis in Tetrahymena Invited Peer-reviewed

O Numata, K Fujiu, K Gonda

CELL BIOLOGY INTERNATIONAL　23　(12)　849-857　1999

DOI： 10.1006/cbir.1999.0481 　

ISSN： 1065-6995

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Books and Other Publications 7

Functionalized nanomaterials II (1st Edition)

Kobayashi Y, Gonda K

Engineering & Technology, Physical Sciences　2021/04
Handbook of Sol-Gel Science and Technology

Kobayashi Y, Gonda K

2016
マイクロ／ナノカプセルの調製、徐放性制御と応用事例

小林芳男, 権田幸祐, 大内憲明

技術情報協　2014
1分子生物学

権田幸祐

化学同人　2014
先端バイオマテリアルハンドブック

樋口秀男, 権田幸祐

NTS出版　2012
乳癌テーラーメード治療の理論と実践

武田元博, 権田幸祐, 大内憲明

金原出版　2009
有機分散系の分散・凝集技術

小林芳男, 権田幸祐, 大内憲明

シーエムシー出版　2008

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Industrial Property Rights 58

蛍光ナノ粒子を用いた、病理学的完全奏効(pCR)の予測を支援するための検査支援方法

郷田秀樹, 高橋優, 高梨健作, 岡田尚大, 尾崎雄一, 吉原由佳, 中野寧, 権田幸祐, 大内憲明, 渡邉みか, 多田寛, 宮下穣, 増田慎三, 戸井雅和

Property Type: Patent
生体物質定量方法、病理診断支援システム及びプログラム

郷田秀樹, 高梨健作, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
生体物質定量方法、病理診断支援システム及びプログラム

磯田武寿, 古澤直子, 元杭康之, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
ソラフェニブを含有する標識剤

相宮拓司, 古澤直子, 川岸直樹, 権田幸祐, 村上圭吾, 渡邉みか, 大内憲明

Property Type: Patent
多重免疫染色法に基づく生体物質の定量方法

郷田秀樹, 磯田武寿, 渡辺泰宏, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
画像処理装置、病理診断支援システム、画像処理プログラム及び画像処理方法

渡辺泰宏, 一谷修司, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
染色方法

高橋優, 中野寧, 岡田文徳, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
染色方法

高橋優, 中野寧, 岡田文徳, 権田幸祐, 大内憲明, 渡邉みか

特許第6237194号

Property Type: Patent
組織染色方法および生体物質検出方法

相宮拓司, 郷田秀樹, 岡田尚大, 中野寧, 権田幸祐, 武田元博, 大内憲明

Property Type: Patent
組織染色方法および生体物質検出方法

相宮拓司, 郷田秀樹, 岡田尚大, 中野寧, 権田幸祐, 武田元博, 大内憲明

特許第6128153号

Property Type: Patent
組織切片における生体物質の定量法

岡田文徳, 相宮拓司, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
画像処理装置、病理診断支援システム、画像処理プログラム及び病理診断支援方法

渡辺泰宏, 磯田武寿, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
生体物質検出方法

高梨健作, 郷田秀樹, 高野敬三, 星野秀樹, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
医用画像処理装置及びプログラム

角森昭教, 岡田尚大, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
医用画像処理装置及びプログラム

角森昭教, 岡田尚大, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
組織染色方法

郷田秀樹, 星野秀樹, 高梨健作, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
生体物質の検出方法

星野秀樹, 郷田秀樹, 高梨健作, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
組織評価方法

岡田尚大, 郷田秀樹, 高梨健作, 相宮拓司, 中野寧, 権田幸祐, 大内憲明, 渡邉みか, 角森昭教, 石光義幸

Property Type: Patent
組織評価方法

岡田尚大, 郷田秀樹, 高梨健作, 相宮拓司, 中野寧, 権田幸祐, 大内憲明, 渡邉みか, 角森昭教, 石光義幸

特許第5887823号

Property Type: Patent
蛍光物質内包ナノ粒子およびこれを用いた生体物質の検出方法

古澤直子, 相宮拓司, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
蛍光物質内包ナノ粒子およびこれを用いた生体物質の検出方法

古澤直子, 相宮拓司, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

特許第5790352号

Property Type: Patent
生体物質発現レベル評価システム

相宮拓司, 角森昭教, 郷田秀樹, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

特許第5906623号

Property Type: Patent
生体物質発現レベル評価システム

相宮拓司, 角森昭教, 郷田秀樹, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
生体物質検出方法

高梨健作, 岡田尚大, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
組織染色方法

郷田秀樹, 岡田尚大, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

Property Type: Patent
X線吸収蛍光ナノ粒子

古澤直子, 相宮拓司, 中野寧, 権田幸祐, 大内憲明, 中川智彦

Property Type: Patent
病理診断情報生成方法及び病理診断情報生成システム

岡田尚大, 郷田秀樹, 中野寧, 角森昭教, 権田幸祐, 武田元博, 大内憲明

Property Type: Patent
病理診断情報生成方法及び病理診断情報生成システム

岡田尚大, 郷田秀樹, 中野寧, 角森昭教, 権田幸祐, 武田元博, 大内憲明

特許第5858461号

Property Type: Patent
免疫組織化学染色方法及び反応試薬

高野敬三, 星野秀樹, 岡田尚大, 中野寧, 権田幸祐, 武田元博, 大内憲明

Property Type: Patent
免疫組織染色法、およびこれを用いた抗体医薬の有効性を判定する方法

郷田秀樹, 中野寧, 岡田尚大, 権田幸祐, 武田元博, 大内憲明

Property Type: Patent
組織評価方法

岡田尚大, 郷田秀樹, 高梨健作, 相宮拓司, 中野寧, 権田幸祐, 武田元博, 大内憲明

Property Type: Patent
抗体を成分として含む医薬品の有効性の判定方法

宮下穣, 権田幸祐, 大内憲明, 武田元博

Property Type: Patent
生体物質検出方法

高梨健作, 郷田秀樹, 岡田尚大, 中野寧, 権田幸祐, 武田元博, 大内憲明

Property Type: Patent
がん発症又はがん発症リスクの判定方法

権田幸祐, 宮下穣, 武田元博, 大内憲明

Property Type: Patent
組織染色方法、組織評価方法および生体物質検出方法

相宮拓司, 郷田秀樹, 岡田尚大, 中野寧, 権田幸祐, 武田元博, 大内憲明

Property Type: Patent
半導体ナノ粒子集積体及び半導体ナノ粒子集積体の製造方法

星野秀樹, 高橋優, 権田幸祐, 武田元博, 大内憲明

Property Type: Patent
半導体ナノ粒子集積体

高橋優, 高梨健作, 星野秀樹, 権田幸祐, 武田元博, 大内憲明

Property Type: Patent
輸入リンパ管流入部検出方法及び特定細胞同定方法

日景允, 権田幸祐, 武田元博, 亀井尚, 大内憲明, 郷田秀樹, 中野寧

Property Type: Patent
がん細胞運動およびがん細胞浸潤抑制剤

権田幸祐, 樋口秀男, 大内憲明, 武田元博

Property Type: Patent
多重免疫染色法に基づく生体物質の定量方法

郷田秀樹, 磯田武寿, 渡辺泰宏, 権田幸祐, 大内憲明, 渡邉みか

特許第5843054号

Property Type: Patent
画像処理装置、病理診断支援システム、画像処理プログラム及び画像処理方法

渡辺泰宏, 一谷修司, 権田幸祐, 大内憲明, 渡邉みか

特許第5822054号

Property Type: Patent
生体物質検出方法

高梨健作, 郷田秀樹, 高野敬三, 星野秀樹, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

特許第6265119号

Property Type: Patent
医用画像処理装置及びプログラム

角森昭教, 岡田尚大, 権田幸祐, 大内憲明, 渡邉みか

特許第5804194号

Property Type: Patent
医用画像処理装置及びプログラム

角森昭教, 岡田尚大, 権田幸祐, 大内憲明, 渡邉みか

特許第5892238号

Property Type: Patent
組織染色方法

郷田秀樹, 星野秀樹, 高梨健作, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

特許第6168047号

Property Type: Patent
生体物質の検出方法

星野秀樹, 郷田秀樹, 高梨健作, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

特許第6194882号

Property Type: Patent
生体物質検出方法

高梨健作, 岡田尚大, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

特許第5812095号

Property Type: Patent
組織染色方法

郷田秀樹, 岡田尚大, 中野寧, 権田幸祐, 大内憲明, 渡邉みか

特許第6127972号

Property Type: Patent
免疫組織染色法、およびこれを用いた抗体医薬の有効性を判定する方法

郷田秀樹, 中野寧, 岡田尚大, 権田幸祐, 武田元博, 大内憲明

特許第5900489号

Property Type: Patent
組織評価方法

岡田尚大, 郷田秀樹, 高梨健作, 相宮拓司, 中野寧, 権田幸祐, 武田元博, 大内憲明

特許第5863057号

Property Type: Patent
生体物質検出方法

高梨健作, 郷田秀樹, 岡田尚大, 中野寧, 権田幸祐, 武田元博, 大内憲明

特許第5974892号

Property Type: Patent
抗体を成分として含む医薬品の有効性の判定方法

宮下穣, 権田幸祐, 大内憲明, 武田元博

特許第5682976号

Property Type: Patent
がん発症又はがん発症リスクの判定方法

権田幸祐, 宮下穣, 武田元博, 大内憲明

特許第5682975号

Property Type: Patent
半導体ナノ粒子集積体の製造方法

星野秀樹, 高橋優, 権田幸祐, 武田元博, 大内憲明

特許第5915529号

Property Type: Patent
組織評価方法

相宮拓司, 郷田秀樹, 岡田尚大, 中野寧, 権田幸祐, 武田元博, 大内憲明

特許第5915531号

Property Type: Patent
半導体ナノ粒子集積体

高橋優, 高梨健作, 星野秀樹, 権田幸祐, 武田元博, 大内憲明

特許第5716029号

Property Type: Patent
輸入リンパ管流入部検出方法及び特定細胞同定方法

日景允, 権田幸祐, 武田元博, 亀井尚, 大内憲明, 郷田秀樹, 中野寧

特許第5698131号

Property Type: Patent
がん細胞運動およびがん細胞浸潤抑制剤

権田幸祐, 樋口秀男, 大内憲明, 武田元博

特許第5483023号

Property Type: Patent

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Research Projects 38

次世代放射光と質量分析イメージングで明らかにする腹部大動脈壁構造と瘤径拡大の関連

梅津道久, 赤松大二朗, 芹澤玄, 鈴木峻也, 権田幸祐

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2024/04/01 - 2027/03/31
非標準的遺伝子四重鎖への効果的薬剤送達を基盤とする新規光線力学療法の開発

北村成史, 石田孝宣, 亀井尚, 多田寛, 金野智浩, 権田幸祐

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2022/04/01 - 2025/03/31
プロテオーム・メタボローム解析による乳癌の新規悪性度評価と代謝標的治療効果予測

多田寛, 権田幸祐, 石田孝宣, 原田成美, 北村成史

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2022/04/01 - 2025/03/31
リンパネットワークのメカノバイオ解析に基づくリンパ節転移機序の解明と診断技術開発

高野真由美, 北村成史, 権田幸祐

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2022/04/01 - 2025/03/31
放射線治療に有用なアブスコパル効果の発現機序解明と応用

権田幸祐, 多田寛, 北村成史

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2022/04/01 - 2025/03/31
慢性関節炎の微小血管塞栓術に関する基礎実験

小黒草太, 高瀬圭, 石幡浩志, 権田幸祐, 太田信, 中村保宏, 大田英揮, 佐藤友美, 鎌田裕基

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2022/04/01 - 2025/03/31
ジラジカル錯体をがん認識光熱変換プローブとするがんの未来型セラノスティクス

壹岐伸彦, 西條芳文, 高橋透, 唐島田龍之介, 権田幸祐

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2022/04/01 - 2025/03/31
重症下肢虚血における組織障害因子の解明と新たな救肢治療法の開発

土田憲, 後藤均, 赤松大二朗, 藤島史喜, 多田寛, 権田幸祐, 綿貫宗則

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2019/04/01 - 2023/03/31

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重症下肢虚血（CLI: Critical Limb Ischemia）は下肢末梢動脈疾患（PAD: Peripheral Artery Disease）のうち安静時疼痛や下肢虚血性潰瘍・壊死を呈する最重症の状態でPADの約3%を占める。CLI患者の生命および下肢自然予後は概して不良である。3年以内に約半数が心血管イベントにより死亡し、下肢に関しては血行再建術を行わなければ約30%が一年以内に大切断を余儀なくされるため何らかの血流改善策が下肢救済のためには必須と考えられている。しかし、その脆弱な患者背景から周術期死亡率が高い上に創傷治癒までに数ヶ月を要する事も多いため医療費の高騰などを含め様々な問題を生んでいる。我々はCLI治療の経験から、下肢虚血を契機とした潰瘍・壊死の形成と進展過程には虚血に加えて何らかの組織障害を惹起する要因が介在するものと推察している。組織障害因子を特定する事が出来れば、虚血が存在していても潰瘍・壊死の発生や拡大を抑制する事が可能になるのではないかと考えた。CLI患者の切断組織を用いて組織障害の機序を明らかにし、CLI治療における新しい治療戦略の確立を目指すことを最終目的として本研究を申請する。本研究の完遂によって‘虚血を背景とした下肢潰瘍・壊疽形成の促進因子は何か’という学術的問いに対する究明が期待される。具体的には以下の課題を設定している。①CLI切断趾を用いた組織障害因子の解明 ②マウス虚血肢モデルを用いた組織障害抑制に向けた新たな治療法の確立
腹部大動脈瘤の拡大速度を規定する病理組織学的因子に関する前向きコホート研究

濱田庸, 後藤均, 赤松大二朗, 今井陽介, 菅原宏文, 権田幸祐

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2019/04/01 - 2023/03/31

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昨年度に報告した通り現状ではコロナウイルス感染拡大により、研究計画遂行に大きな支障が生じている。今年度は遅れを取り戻すべく、昨年度と同様に大動脈瘤の拡大速度を規定する病理組織学的因子を解析するため、大動脈瘤血流シミレーション技術の確立と習得、大動脈瘤症例の瘤径の継時的変化の観察、また、大動脈瘤壁の組織サンプルの蓄積、得た技術をもとにした大動脈瘤破裂症例の血流シミレーション等を行う予定であった。しかしながら、今年度も引き続いてコロナ感染は拡大に歯止めがかからず、数回の波も出現した。研究代表者が所属するのが大規模医療機関である事から、大動脈瘤血流シミレーションソフトを開発する予定であった神戸大学との共同研究が、人的交流を含めて困難である状況は変わっていない。それゆえ、現在に至るまでもシミレーションの基盤を確立する段階に至っていない。オンラインミーティングを活用もしているものの、オンラインではその特性からプライバシーに配慮した患者データのやり取りには限界があり、それを元にしたシミレーションデモは行っているものの、数名程度にとどまっている。また、引き続きコロナウイルス感染症の影響で、そもそも大動脈瘤の新規受診症例が減少していることは変わりなく、また、ICU病床・手術室の利用制限も同様であるため、大動脈瘤手術症例の蓄積を行う事が困難である事にも変わりがない。したがって今年度も研究実績は、残念ながらほぼ得られていない。コロナウイルス感染症は収束の糸口が若干見えてきた感があるが、残りの研究期間を考えると、現時点で当初予定していた研究遂行は非常に困難であると言わざるを得ない。従って、次年度最終年度を迎えるにあたり、研究期間の延長申請を行う事を考えている。
Development of new cancer therapy by tumor blood vessel-specific radiation therapy

GONDA Kohsuke

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Tohoku University

2019/06/28 - 2022/03/31

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In this study, we aimed to develop a method for improving the antitumor effect of an angiogenesis inhibitor (bevacizumab). In 2019, conditions for low-dose radiation therapy to be used in combination therapy with bevacizumab administration were examined. In addition, the tumor-bearing mice administered with gold nanoparticles (AuNPs) were irradiated with radiation, and radiosensitization effect of AuNPs on tumor shrinkage was investigated. In 2020, to evaluate the histological effect of combination therapy of angiogenesis inhibitor and radiation therapy, we developed techniques to investigate the "morphological change of tumor blood vessels" and "functional change of tumor blood vessels". In 2021, those evaluation technologies developed in 2020 were applied to the evaluation of the antitumor effect of mice in combination with angiogenesis inhibitors and radiation therapy.
Development of early-stage cancer detection method and radiotherapy using ultra-small gold nanoparticles

Kitamura Narufumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2019/04/01 - 2022/03/31

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We prepared ultra-small gold nanoparticles with a diameter of approximately 3 nm by coating with reduced glutathione. In animal experiments using mice, it was confirmed that the nanoparticles could be excreted in urine with a half-life of less than one hour in blood. The initial plan was then slightly modified to integrate multiple gold nanoparticles on a protein basis rather than iron oxide nanoparticles. As a result, biodegradable nanoparticles with 3~4 gold nanoparticles bound on protein particles of about 10~20 nm in diameter were successfully synthesized. The results of CT imaging analysis showed that synthesized nanoparticles had different blood convection times depending on the size and properties of the scaffold protein. The gold nanoparticles can be used as a contrast agent to delineate tiny blood vessels in tumors.
Development and application of new gastrointestinal fluorescent clip system which can be detected from outside

Kamei Takashi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2017/04/01 - 2022/03/31

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The aim of this research was development of a new fluorescent endoscopic clip system which is detected through gastrointestinal wall and we had the study results as below. 1. We have developed and improved a new fluorescent endoscopic system. 2. We created an original fluorescent clip system with PID, new organic fluorescent probe, and analyzed the property of this clip. 3. We successfully detected the fluorescent signal of the clips from outside of the gastrointestinal tract (esophagus, stomach, colon) in pig model using our original surgical system. 4. We confirmed that gastrointestinal mucosa was not affected by the clip from the pathological examination. 5. We also confirmed that there was no significant change of fluorescent property in the examination of the effects with acid or alkali assuming digestive juice.
Clinical significance of ABCG2/BCRP quantified by fluorescence nanoparticle in breast cancer patients undergoing neoadjuvant chemotherapy

TADA Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2017/04/01 - 2022/03/31

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We aimed to evaluate the correlation between BCRP expression and prognosis in breast cancer tissue samples using immunohistochemistry with fluorescent phosphor-integrated dots (IHC-PIDs). A total of 37 breast cancer patients with residual cancer in the primary tumor and axillary lymph node were evaluated. BCRP levels in breast cancer tissue (PT) and metastatic lymph nodes (LN) were quantitatively detected after preoperative chemotherapy (NAC) and core needle biopsy (CNB). Biomarker assay with IHC-PIDs showed high accuracy for quantitative assessment of BCRP with low expression. High BCRP expression in Primary tumor and lymphnode after NAC was associated with worse overall survival (log-rank, p=0.0089). In conclusion, high BCRP levels are associated with poor prognosis in breast cancer patients with residual tumor in the primary tumor and lymph node after preoperative chemotherapy. These findings provide a basis for further appropriate adjuvant therapy in these patients.
薬剤による腫瘍血管の病態変化を可視化する技術開発

権田幸祐

Offer Organization: 日本医療研究開発機構

System: シーズA)開発

Institution: 東北大学

2021/04 - 2022/03
Investigation of early diagnosis and drug delivery using gold nanoparticle for mice model of colorectal liver metastases.

Nishimura Ryuichi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2018/04/01 - 2021/03/31

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Micro cancers in mouse models of colorectal cancer liver metastasis (CRCLM) and hepatocellular carcinoma (HCC) were detected using high-resolution CT imaging device with gold nanoparticle contrast agent. The in vivo CT imaging showed that there is a difference between the EPR effects of the micro cancers in HCC and in CRCLM. We succeeded to obtain pathological sections of the micro cancers and immunohistochemistry of them were performed using angiogenesis markers of CD31 and αSMA. As the result, it was suggested that differences in angiogenesis affect drug delivery in early stage of cancer.
薬剤による腫瘍血管の病態変化を可視化する技術開発

権田幸祐

Offer Organization: 日本医療研究開発機構

System: シーズA)開発

Institution: 東北大学

2020/04 - 2021/03
High Resolusion Imaging and Pathological Analysis of Micro Tumor in Mouse Model for Non-alcoholic Steatohepatitis-associated Hepatocellular Carcinoma

Hara Yasuyuki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2016/04/01 - 2019/03/31

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Hepatocellular carcinoma (HCC), particularly originated from the background of non-alcoholic steatohepatitis (NASH), has been on the rise in recent years. The carcinogenic mechanisms of NASH-HCC is difficult to understood because of its complications such as diabetes mellitus and hyperlipidemia. Then, early diagnosis of NASH-HCC is very important. We attempted to detect early lesions in NASH-HCC model mice by using gold nanoparticle contrast agents. By using the EPR (Enhanced Permeability and Retention Effect) of gold nanoparticles, it was actually successfully detected a micro-cancer less than 1 mm. Furthermore, pathological analysis according to the degree of progression was possible by removing those small lesions as pathological sections.
Elucidation of infiltration and propagation mechanism of breast cancer bone metastasis using multimodal imaging

Sakurai Yu

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2015/04/01 - 2019/03/31

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Bone metastasis is a high frequency as a metastatic site of cancer like breast, lung and prostate cancer. It is important to diagnose and medical treatment of the bone metastasis at early stage because of many clinically problematic symptoms such as pain and hypercalcemia caused by pathological fractures. Using a cancer-bearing mouse model that causes voluntarily bone metastasis, we measured the bone density of the osteocortex and bone marrow. The researh results showed us that at the beginning of the bone metastasis, the bone marrow metastasis was occurred ahead to the osteocortex metastasis. It is considered useful for postoperative periodic examination because it can be measured at low cost and with no exposure. In this study we could diagnose early bone marrow metastasis and it seemed to be effective in elucidation of the mechanism of invasion and propagation of bone metastasis.
リンパ節がん微小転移の高精度診断法の開発 Competitive

権田幸祐

Offer Organization: 日本学術振興会

System: 基盤研究 (B)

2016/04 - 2019/03
ナノバイオ材料を用いた高分解能イメージングによるがん生物学の主要分子機序の解明 Competitive

権田幸祐

Offer Organization: 日本学術振興会

System: 日中韓フォーサイト事業

2013/08 - 2018/07
がんの検出と治療の効果を同時に併せ持つナノ粒子造影剤の開発 Competitive

権田幸祐

Offer Organization: 日本学術振興会

System: 挑戦的萌芽

2016/04 - 2018/03
quantitative diagnosis of Breast cancer stemness-related protein by single particle nano imaging

Hiroshi Tada, Nemoto Noriko

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2014/04/01 - 2017/03/31

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Assessing breast cancer tissues for expression of cancer stemness biomarkers, such as breast cancer resistance protein (BCRP) which is a member of ABC transporter, may provide critical information for patient prognosis. We developed a novel immunohistochemical method with new fluorescent nanoparticle, phosphor integrated dot (PID), by single-particle imaging using tissue samples from patients with clinical information. We successfully calculated the number of PID particles in a cell and defined it as nano patho score that reflects the level of BCRP protein expression in cancer cells. The BCRP nano patho score in 13 cases was correlated proportionally with the score of Ki67 as assessed by immunohistochemical method (R =0.62). But BCRP nano patho score were not correlated with breast cancer recurrence rate in breast cancer patients who were treated with preoperative chemotherapy
Basic construction for treatment effect prediction of molecular targeted drugs by quantitative estimation of HER family receptor based on highly sensitive nanoparticle imaging

Ohuchi Noriaki

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tohoku University

2013/04/01 - 2017/03/31

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We developed new method for quantitative imaging of HER2/HER3 dimer by using fluorescent nanoparticle probes conjugated with antibodies. We performed detecting HER2/HER3 hetero-dimers by using the combination of antibody-based fluorescent probes which induce fluorescent resonance energy transfer (FRET). Because the efficiency of FRET is extremely sensitive to the distance between the pair of fluorophores, this method can be suitable for our purpose. We have applied this method for detection of HER2/HER3 hetero-dimers expressed on the membranes of human breast cancer cell lines. The amount of HER2/HER3 hetero-dimers and localization on cultured-cell lines have been evaluated with quantitative sensitivity. The imaging data showed HER2 and/or HER3-derived unique localization, and hot-spot on cancer cells.We also developed the evaluation method of the effect of antibody-drugs, especially anti-HER2 dimerization inhibitor, by FRET technique.
X-ray computed tomography imaging of a tumor with high sensitivity

Ohuchi Noriaki, GONDA Kohsuke, TADA Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2014/04/01 - 2016/03/31

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Contrast agents are often used to enhance the contrast of X-ray computed tomography (CT) imaging of tumors to improve diagnostic accuracy. However, because the iodine-based contrast agents currently used in hospitals are of low molecular weight, the agent is rapidly excreted from the kidney. This leads to nonspecific enhancement of contrast images for tissues. Here, we created gold (Au) nanoparticles as a new contrast agent to specifically image tumors with CT using an enhanced permeability and retention (EPR) effect. Au has a higher X-ray absorption coefficient than does iodine. Au nanoparticles were supported with polyethylene glycol (PEG) chains on their surface to increase the blood retention The developed Au nanoparticles were injected into tumor-bearing mice, and the distribution of Au was examined with CT imaging, transmission electron microscope (TEM) imaging. The results show that specific localization of the developed Au nanoparticles in the tumor
Diagnostics development of hepatocellular carcinoma by ultrahigh precision fluorescent imaging of tyrosine kinase receptor

Hara Yasuyuki, OHUCHI Noriaki, KAWAGISHI Naoki, UMEDA Mika, GONDA Kousuke, TAKEDA Ikuo, SEKIGUCHI Satoru

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2013/04/01 - 2016/03/31

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In this study, we aim to develop the new method to measure the level of expression of sorafenib-targeting proteins by fluorescent nanoparticle quantitatively with a high degree of accuracy. We combined pegylated fluorescent nanoparticle with sorafenib via linker, and confirmed by infrared spectroscopy that synthesized nanoparticle had predictable structure. From the results of experiments using cultured cells, bond between sorafenib and fluorescent nanoparticle did not decrease the inhibitory capacity of cell proliferation, which sorafenib had originally. This showed that combination via linker did not decrease EFGR-binding capacity of sorafenib. In pathological analyses using paraffin-embedded cells, it was difficult to adjust photographing conditions, which was left open.
Nanomedicine Molecular Science

ISHIHARA KAZUHIKO, HIGUCHI Hideo, IWATA Hiroo, NATSUME Atsushi, URISU Tsuneo, FUKUDA Norio, YUI Nobuhiko, MARUYAMA Atsushi, MIYAKE Jun, GONDA Kosuke

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Institution: The University of Tokyo

2011/04/01 - 2016/03/31

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Nanomedicine molecular science focuses on molecular reactions in the cells, which constitute the living organism and control its biological activities. The cellular environment, however, differs from the chemical reaction environment. The goal of this project is to develop molecular reaction parameters to quantify and examine such reactions. The studies are part of a scientific initiative focusing on cells and have the following main objectives: establishing the theoretical basis of molecular reactions in the cell, and understanding the cellular environment and intracellular chemical reaction mechanisms. They include topics related to the origin of cellular functions, which provide knowledge indispensable for molecular nanomedicine; topics required for establishing molecular nanomedicine; topics that may help integrate the research projects and promote constructive interactions among researchers; and topics that encourage original ideas to solve difficulties in the field.
がんリンパ行性転移の分子機構解明に基づく新治療法創発 Competitive

権田幸祐

Offer Organization: 文部科学省

System: 新学術領域研究(研究領域提案型)

2011/04 - 2016/03
Development of Synthesis of Nano-Capsules for Medical Imaging Diagnoses Using Dispersion/Aggregation Process

KOBAYASHI YOSHIO, SATO Masahide, SUZUKI Noboru, GONDA Kohsuke, GONDA Kohsuke

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Ibaraki University

2012/04/01 - 2015/03/31

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Luminescent semiconductor nanoparticles (QDs) are expected to be used as a fluorescent marker for medical diagnostics. Among various methods for reducing harm of Cd contained in QD, encapsulating, or coating of contrast agents with materials inert to living bodies is a good candidate. Meanwhile, materials that have both properties of fluorescence and X-ray absorption will be used for imaging targets by multiple techniques. The present work developed methods for preparing the QDs coated with silica (QD/SiO2), the QD/SiO2 particles on which Au nanoparticles were immobilized (QD/SiO2/Au particles), and the QD/SiO2/Au particles coated with silica (QD/SiO2/Au/SiO2 multi-layered particles).
エピジェネティック異常の超高精度蛍光イメージングによるがん悪性度診断法開発 Competitive

権田幸祐

Offer Organization: 日本学術振興会

System: 基盤研究 (B)

2012/04 - 2015/03
１粒子蛍光ナノイメージングによる超高精度がん組織診断システムの研究開発 Competitive

兒玉篤

Offer Organization: 新エネルギー・産業技術総合開発機構

System: がん超早期診断・治療機器の総合研究開発

2010/09 - 2015/03
生体内超微細構造可視化技術を用いた抗体医薬品の革新的創薬法の開発 Competitive

権田幸祐

Offer Organization: 日本学術振興会

System: 若手研究 (B)

2010/04 - 2012/03
Development of an innovative method for evaluating DDS formulationpharmacokinetics by using multi-photon excitation microscopy

OUCHI Noriaki, GONDA Kosuke, AMARI Masakazu, TADA Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2011 - 2012

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We prepared GFP-fused EB1-expressing human breast cancer cells. The growth rate of the microtubule plus-end was measured using the positions of EB1 assembly over time. Analysis of the dynamics of the EB1-GFP comet showed that the microtubule growth rates decreased when the cells were treated with anticancer drugs. We also measured the microtubule growth rates in tumor-bearing mice by using an original stereotaxic instrument. Thus, alteration in EB1-GFP dynamics is an effective indicator for the effects of anticancer drugs.
Development of a novel sentinel lymph node biopsy method using fluorescent nano-particles

KAMEI Takashi, OHCHI Noriaki, GONDA Kosuke, TANIYAMA Yusuke

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2010 - 2012

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We succeeded in real-time fluorescent imaging of sentinel lymph nodes of various gastrointestinal tracts in pig model using QDs as a tracer. After that, we analyzed fluorescent specificities in varied conditions and revealed the range of a signal detection through fat tissue or other organs. Furthermore, we developed novel organic material nanoparticles as a safety fluorescent probe for sentinel lymph node biopsy(SLNB) with the idea of clinical application. We revealed that these particles were suitable for SLNB and their fluorescent specificities were similar to the QDs.
蛍光ナビゲーション内視鏡による消化器癌手術システムの開発

武田元博, 亀井尚, 権田幸祐, 大内憲明, 里見進, 日景允

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 挑戦的萌芽研究

Institution: 東北大学

2010 - 2011

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内視鏡下手術は、低侵襲性、術後回復の早さと速やかな社会復帰、優れた整容性などの利点がある。私共は蛍光イメージングを内視鏡手術に付加し、新規の生体情報を蛍光シグナルで提供できる内視鏡手術システムを構想した。私共はこれまでに生体微弱光計測の技術を背景として、蛍光の体外診断装置の開発、小型動物を用いた蛍光検出実験を行い、また培養細胞やマウスにおいて蛍光量子ドットを用い、ナノレベルの超微細蛍光計測システムを開発、in vivoリアルタイム観察することに成功している。本研究の目的は、これら超精細蛍光計測技術を基に、腫瘍の存在部位同定と切除範囲の決定、脈管(胆管、胸管など)走行の確認と副損傷回避、センチネルリンパ節描出などを術中リアルタイムに蛍光シグナルとして得ることで、詳細な生体情報を提供するシステムを構築することである。今年度はブタ消化管をモデルとして蛍光計測を行い、量子ドット投与量と蛍光強度について相関関係を得ることができた。また、蛍光ナノ粒子注入直後より蛍光ナノ粒子がセンチネルリンパ節に移動する様子をリアルタイムに観察、染色されたセンチネルリンパ節の精密蛍光計測により、リンパ管流入部位を高精度に同定することに成功した(Hikage,Nanotechnology,2010)。これによりリンパ経路が複雑かつ多方向に及ぶ可能性のある、胃をはじめとした消化管がんの術中センチネルリンパ節生検等に応用が期待される。また生体内の蛍光ナノ粒子の安定化を目指し、シリカコーティングした蛍光ナノ粒子の安定した作製に成功した(Cong,PLoS ONE,2010)。この技術により将来安全な蛍光マーカーの開発が期待される。以上の成果により今後、新規蛍光ナビゲーション内視鏡下手術による、より緻密で安全な内視鏡手術の開発が期待される。
癌疾患モデルマウスのin vivoナノイメージング法の開発とナノ医療への応用 Competitive

権田幸祐

Offer Organization: 科学技術振興機構

System: シーズ発掘試験研究

2009/07 - 2010/03
新規抗体医薬品と革新的免疫染色法を用いたがん治療法の開発 Competitive

権田幸祐

Offer Organization: 日本学術振興会

System: 挑戦的萌芽

2009/04 - 2010/03
転移性乳がん細胞を標的とした抗がん剤の開発と応用 Competitive

権田幸祐

Offer Organization: 科学技術振興機構

System: シーズ発掘試験研究

2008/07 - 2009/03
電位依存性Ca2+チャンネルの新規調節因子Centrinの分子機能の解明 Competitive

権田幸祐

Offer Organization: 日本学術振興会

System: 若手研究(B)

2005/04 - 2007/03

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