Details of the Researcher

PHOTO

Wataru Makino
Section
Graduate School of Life Sciences
Job title
Assistant Professor
Degree

  • 博士（水産学）（北海道大学）

  • 修士（水産学）（北海道大学）

Researcher ID
GLS-2518-2022

Research History 4

  • 2002/04 - 2003/09
    日本学術振興会 特別研究員

  • 2000/02 - 2002/03
    ミネソタ大学 生態・進化・行動学部 研究員

  • 1999/05 - 2000/01
    愛媛大学沿岸環境科学研究センター 講師（研究機関研究員）

  • 1998/04 - 1999/03
    （財）水産科学研究奨励会 研究員

Education 2

  • Hokkaido University　Faculty of Fisheries

    - 1998/03/25

  • Hokkaido University　Faculty of Fisheries

    - 1993/03/25

Committee Memberships 16

  • 日本陸水学会　評議員（東北地区委員）

    2024/01 - Present

  • 日本プランクトン学会　日本プランクトン学会報 編集委員

    2021/04 - Present

  • 一般財団法人 水源地環境センター　河川水辺の国勢調査「ダム湖版」スクリーニング委員会

    2016/12 - Present

  • 日本陸水学会・英文誌編集委員会　委員

    2017/01 - 2022/12

  • 日本陸水学会　評議員（東北地区委員）

    2018/01 - 2021/12

  • 日本生態学会・Ecological Research編集委員　Handling editor

    2016/01 - 2019/12

  • 日本生態学会・東北地区会　庶務幹事

    2016/08 - 2018/07

  • 日本陸水学会　日本陸水学会大会 第82回仙北市田沢湖大会実行委員会

    2017/09 - 2017/10

  • 日本生態学会・東北地区会　庶務幹事

    2014/08 - 2016/07

  • 日本陸水学会・和文誌編集委員会　委員

    2011/01 - 2014/12

  • 日本生態学会・東北地区会　会計幹事

    2010/08 - 2012/07

  • 日本陸水学会・英文誌編集委員会　委員

    2005/01 - 2008/12

  • 日本生態学会・東北地区会　会計幹事

    2004/08 - 2006/07

  • 日本生態学会・東北地区会　会計幹事

    2004/08 - 2006/07

  • 日本生態学会大会 第70回仙台大会実行委員会

    2023/03 -

  • 日本生態学会　日本生態学会大会 第63回仙台大会実行委員会

    2016/03 -

Show all ︎Show first 5

Professional Memberships 5

  • American Society of Limnology and Oceanography

  • Japanese Society of Limnology

  • 日本進化学会

  • Plankton Society of Japan

  • Japanese Society of Ecology

Research Interests 6

  • ecological stoichiometry

  • phylogeography

  • life history strategy

  • distribution

  • plankton

  • DNA baracode

Research Areas 1

  • Life sciences / Ecology and environmental science /

Awards 3

  1. Best Paper Award of the Plankton Society of Japan

    2022/03　Feeding ecology of a mysid species, Neomysis awatschensis in the Lake Kasumigaura: combining approach with microscopy, stable isotope analysis and DNA metabarcoding

  2. English Oral Presentation Audience Special Award

    2021/03　Untangling lineages of freshwater copepods having a broad geographical range: The cases of Cyclops vicinus and C. kikuchii

  3. 日本陸水学会 第6回学会賞（吉村賞）

    2004/09　日本陸水学会　Limnology誌に掲載された論文に対して

Papers 73

  1. The first report of the non-indigenous Chydorus brevilabris Frey, 1980 (Crustacea: Cladocera) in Asian freshwaters Peer-reviewed

    Wataru Makino, Hiromichi Suzuki, Yurie Otake, Syuhei Ban, Jotaro Urabe

    Limnology　2023/04/27

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10201-023-00719-4 　

    ISSN： 1439-8621

    eISSN： 1439-863X

  2. Comparative phylogeography of Cyclops vicinus Uljanin, 1875 and Cyclops kikuchii Smirnov, 1932 with implications for differences in their ecological characteristics Peer-reviewed

    Imane Sioud, Wataru Makino, Jotaro Urabe

    Journal of Plankton Research　43　(6)　974-985　2021/11

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/plankt/fbab076 　

    ISSN： 0142-7873

    eISSN： 1464-3774

  3. Differential intraspecific genetic variations of the closely related, wide-ranged freshwater copepods Cyclops vicinus Uljanin, 1875 and C. kikuchii Smirnov, 1932 Peer-reviewed

    Imane Sioud, Wataru Makino, Jotaro Urabe

    Limnology　22　(2)　209-219　2021/04

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10201-020-00647-7 　

    ISSN： 1439-8621

    eISSN： 1439-863X

  4. Feeding ecology of a mysid species, Neomysis awatschensis in the Lake Kasumigaura: combining approach with microscopy, stable isotope analysis and DNA metabarcoding Peer-reviewed

    Yasuhide Nakamura, Akihiro Tuji, Wataru Makino, Shin-ichiro S. Matsuzaki, Nobuaki Nagata, Megumi Nakagawa, Noriko Takamura

    Plankton and Benthos Research　15　(1)　44-54　2020/02/28

    Publisher: The Plankton Society of Japan/The Japanese Association of Benthology

    DOI： 10.3800/pbr.15.44 　

    ISSN： 1880-8247

    eISSN： 1882-627X

  5. Underestimated species diversity and hidden habitat preference in Moina (Crustacea, Cladocera) revealed by integrative taxonomy Peer-reviewed

    Wataru Makino, Ryuji J. Machida, Jiro Okitsu, Nisikawa Usio

    Hydrobiologia　847　(3)　857-878　2019/12/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10750-019-04147-3 　

    ISSN： 0018-8158

    eISSN： 1573-5117

  6. The taxonomic position of Asian Holopedium (Crustacea: Cladocera) confirmed by morphological and genetic analyses Peer-reviewed

    Ayaka Yamamoto, Wataru Makino, Jotaro Urabe

    Limnology　21　(1)　97-106　2019/07/11

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10201-019-00585-z 　

    ISSN： 1439-8621

    eISSN： 1439-863X

  7. Evidence of multiple introductions and genetic admixture of the Asian brush-clawed shore crab Hemigrapsus takanoi (Decapoda: Brachyura: Varunidae) along the Northern European coast Peer-reviewed

    Wataru Makino, Osamu Miura, Felix Kaiser, Mélanie Geffray, Tatsuya Katsube, Jotaro Urabe

    Biological Invasions　20　(4)　825-842　2018/04/01

    Publisher: Springer International Publishing

    DOI： 10.1007/s10530-017-1604-0 　

    ISSN： 1573-1464 1387-3547

  8. The fauna of freshwater calanoid copepods in Japan in the early decades of the 21st Century: Implications for the assessment and conservation of biodiversity Peer-reviewed

    Wataru Makino, Akifumi S. Tanabe, Jotaro Urabe

    Limnology and Oceanography　63　(2)　758-772　2018/03/01

    Publisher: Wiley Blackwell

    DOI： 10.1002/lno.10667 　

    ISSN： 1939-5590

  9. DNA barcoding of freshwater zooplankton in Lake Kasumigaura, Japan Peer-reviewed

    Wataru Makino, Natsumi Maruoka, Megumi Nakagawa, Noriko Takamura

    ECOLOGICAL RESEARCH　32　(4)　481-493　2017/07

    DOI： 10.1007/s11284-017-1458-z 　

    ISSN： 0912-3814

    eISSN： 1440-1703

  10. Cryptic diversity of Japanese Diaphanosoma (Crustacea: Cladocera) revealed by morphological and molecular assessments Peer-reviewed

    Csilla Lakatos, Jotaro Urabe, Wataru Makino

    INLAND WATERS　5　(3)　253-262　2015

    DOI： 10.5268/IW-5.3.847 　

    ISSN： 2044-2041

    eISSN： 2044-205X

  11. Finding copepod footprints: A protocol for molecular identification of diapausing eggs in lake sediments Peer-reviewed

    Wataru Makino, Hajime Ohtsuki, Jotaro Urabe

    Limnology　14　(3)　269-282　2013/08

    DOI： 10.1007/s10201-013-0404-1 　

    ISSN： 1439-8621

  12. Invasion, genetic variation and species identity of the calanoid copepod Sinodiaptomus valkanovi Peer-reviewed

    Wataru Makino, Matthew A. Knox, Ian C. Duggan

    FRESHWATER BIOLOGY　55　(2)　375-386　2010/02

    DOI： 10.1111/j.1365-2427.2009.02287.x 　

    ISSN： 0046-5070

  13. Extreme population genetic differentiation and secondary contact in the freshwater copepod Acanthodiaptomus pacificus in the Japanese Archipelago Peer-reviewed

    Wataru Makino, Akifumi S. Tanabe

    MOLECULAR ECOLOGY　18　(17)　3699-3713　2009/09

    DOI： 10.1111/j.1365-294X.2009.04307.x 　

    ISSN： 0962-1083

    eISSN： 1365-294X

  14. Niche partitioning and phylogenetic distance of rotifer species revealed by the four-year temporal dynamics in a small mountain lake Peer-reviewed

    Ishara Uhanie Perera, Natsumi Maruoka, Xiaofei Tian, Wataru Makino, Jotaro Urabe

    Limnology　2024/11/30

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10201-024-00771-8 　

    ISSN： 1439-8621

    eISSN： 1439-863X

  15. High-resolution Microscopic Image Dataset of Freshwater Plankton in Japanese Lakes and Reservoirs (FREPJ): I. Zooplankton Peer-reviewed

    Yurie Otake, Aoi Osone, Wataru Makino, Koichi Ito, Takafumi Aoki, Kanta Miura, Yoshinobu Hayakawa, Ryotaro Yoshida, Satoshi Ichise, Akihiro Tuji, Jotaro Urabe

    Bulletin of the National Museum of Nature and Science. Series B, Botany　50　(4)　159-164　2024/11

    DOI： 10.50826/bnmnsbot.50.4_159 　

  16. Taxonomic re-evaluation of the Brachionus calyciflorus species complex (Rotifera) in Japanese freshwaters Peer-reviewed

    Yurie Otake, Mai Kurokawa, Natsumi Maruoka, Megumi Nakagawa, Wataru Makino, Jotaro Urabe

    Limnology　2024/10/18

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s10201-024-00768-3 　

    ISSN： 1439-8621

    eISSN： 1439-863X

    More details Close

    Abstract Recently, the Brachionus calyciflorus species complex was divided into four nominal species: B. calyciflorus sensu stricto (s.s.) Pallas, 1766; Brachionus dorcas Gosse, 1851, Brachionus elevatus Michaloudi et al., 2018, and Brachionus fernandoi Michaloudi et al., 2018 based on the results of both morphological and molecular investigations. We applied this systematic approach to B. “calyciflorus” collected from lakes and ponds in various parts of Japan to examine spatial and temporal distributions of these species. We found that all four species indeed distributed in Japan. Among the species, B. fernandoi exhibited a somewhat different occurrence pattern and morphology. Generally, B. fernandoi appeared to occur in the winter–spring, while the other three species seemed to occur in the early summer–late autumn. A seasonal succession from B. fernandoi-dominant community to other species-dominant communities was observed in the same lakes/ponds. A statistical analysis showed that B. fernandoi-dominant species composition was also related to low temperatures. It was also found that the body of B. fernandoi was more elongated compared to those of other species. These results suggest that B. fernandoi is a distinct species in terms of morphology and phenology. Among the other three species, we observed a variation in the length of posterolateral spines. However, it was not clear if this variation is a trait that distinguish these species morphologically. In addition, some of these species co-occurred in the same lakes and ponds. The mechanism by which they coexist is a topic for future study.

  17. Assessment of toxic effects of imidacloprid on freshwater zooplankton: An experimental test for 27 species Peer-reviewed

    Hiromichi Suzuki, Wataru Makino, Shinji Takahashi, Jotaro Urabe

    Science of The Total Environment　927　172378-172378　2024/06

    Publisher: Elsevier BV

    DOI： 10.1016/j.scitotenv.2024.172378 　

    ISSN： 0048-9697

  18. To lose is to win: Long‐term co‐occurrence of two asexual populations realized by a dormant strategy of the inferior competitor Peer-reviewed

    Natsumi Maruoka, Kenyu Yamaki, Wataru Makino, Jotaro Urabe

    Functional Ecology　2024/04/08

    Publisher: Wiley

    DOI： 10.1111/1365-2435.14551 　

    ISSN： 0269-8463

    eISSN： 1365-2435

    More details Close

    Abstract Asexual organisms are ubiquitous. While being members of a single species with the same ecological requirements, multiple asexual genotypes within a species are often found in a single habitat. Niche partitioning in time and space among genotypes has been proposed to explain this phenomenon. However, it is not clear whether these different genotypes co‐occur in the long term. Therefore, we examined the population dynamics of two asexual Daphnia cf. pulex genotypes (JPN1 and JPN2) over 9 years in a small mountain lake. These two genotypes consistently occurred in the same seasons and layers, suggesting that niche partitioning cannot explain their long‐term co‐occurrence. The abundance of JPN1 was typically higher in most years. However, the abundance of dormant eggs in lake sediments was at the same level between the genotypes. Results of a laboratory experiment showed that JPN1 competitively excluded JPN2. However, many JPN2 individuals produced dormant eggs before JPN1 competitively excluded them from the experiment. Furthermore, the competitively inferior JPN2 produced dormant eggs abundantly in the medium containing a crowding cue from JPN1 while no such trend was observed for JPN1. These results showed that a competitively inferior asexual genotype could maintain a population with a competitively superior genotype for a long period of time because it had the ability to detect an increase in competitors and produce dormant eggs each year before being competitively eliminated. Based on these results, we suggest that the variation in genotype‐specific response in dormant egg production to environmental change plays a key role in the long‐term co‐occurrence of different asexual genotypes in single habitats. Read the free Plain Language Summary for this article on the Journal blog.

  19. Zooplankton Classification Using Hierarchical Attention Branch Network

    Koichi Ito, Kanta Miura, Takafumi Aoki, Yurie Otake, Wataru Makino, Jotaro Urabe

    Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)　14407 LNCS　409-419　2023

    DOI： 10.1007/978-3-031-47637-2_31 　

    ISSN： 0302-9743

    eISSN： 1611-3349

  20. Recovery of macrobenthic communities in tidal flats following the Great East Japan Earthquake Peer-reviewed

    Takeshi Yuhara, Takao Suzuki, Tatsuki Nishita, Junichi Murakami, Wataru Makino, Gen Kanaya, Kyoko Kinoshita, Natsuru Yasuno, Takashi Uchino, Jotaro Urabe

    Limnology and Oceanography Letters　8　(3)　473-480　2022/11/10

    Publisher: Wiley

    DOI： 10.1002/lol2.10292 　

    ISSN： 2378-2242

    eISSN： 2378-2242

  21. Temporal β-diversity of zooplankton at various time scales in a small mountain lake Peer-reviewed

    Ishara Uhanie Perera, Natsumi Maruoka, Wataru Makino, Jotaro Urabe

    Limnology　22　(1)　89-99　2021/01

    DOI： 10.1007/s10201-020-00637-9 　

    ISSN： 1439-8621

    eISSN： 1439-863X

  22. Variations in effects of ectosymbiotic microbes on the growth rates among different species and genotypes of Daphnia fed different algal diets Peer-reviewed

    Xuan Zhang, Hajime Ohtsuki, Wataru Makino, Yasuhiko Kato, Hajime Watanabe, Jotaro Urabe

    Ecological Research　36　(2)　303-312　2020/12/29

    Publisher: Wiley

    DOI： 10.1111/1440-1703.12194 　

    ISSN： 0912-3814

    eISSN： 1440-1703

  23. Nuclear and mitochondrial ribosomal ratio as an index of animal growth rate Peer-reviewed

    Wye‐Lup Kong, Takeshi Miki, Ya‐Ying Lin, Wataru Makino, Jotaro Urabe, Shi‐Hong Gu, Ryuji J. Machida

    Limnology and Oceanography: Methods　17　575-584　2019/11

    DOI： 10.1002/lom3.10334 　

  24. Resting eggs of the perennial copepod Eodiaptomus japonicus in Lake Biwa (Japan) Peer-reviewed

    Liu X, Ban S, Deyrend D, Dur G, Kuwae M, Makino W, Urabe J

    Inland Waters　10　(1)　89-100　2019/09

    Publisher: Informa UK Limited

    DOI： 10.1080/20442041.2019.1671766 　

    ISSN： 2044-2041

    eISSN： 2044-205X

  25. Correction to: Evidence of multiple introductions and genetic admixture of the Asian brush-clawed shore crab Hemigrapsus takanoi (Decapoda: Brachyura: Varunidae) along the Northern European coast

    Wataru Makino, Osamu Miura, Felix Kaiser, Mélanie Geffray, Tatsuya Katsube, Jotaro Urabe

    Biological Invasions　20　(5)　1369-1369　2018/05/19

    Publisher: Springer Nature

    DOI： 10.1007/s10530-018-1682-7 　

  26. Rediscovery after almost 120 years: morphological and genetic evidence supporting the validity of Daphnia mitsukuri (Crustacea: Cladocera) Peer-reviewed

    Natsumi Maruoka, Hajime Ohtsuki, Wataru Makino, Jotaro Urabe

    Zoological Science　35　(5)　468-475　2018

    DOI： 10.2108/zs170081 　

    ISSN： 0289-0003

  27. Reconstructing the Invasion History of the Asian shorecrab, Hemigrapsus sanguineus (De Haan 1835) in the Western Atlantic (vol 164, 47, 2017) Peer-reviewed

    April M. H. Blakeslee, Yumi Kamakura, Jaclyn Onufrey, Wataru Makino, Jotaro Urabe, Susan Park, Carolyn L. Keogh, A. Whitman Miller, Mark S. Minton, James T. Carlton, Osamu Miura

    MARINE BIOLOGY　164　(4)　2017/04

    DOI： 10.1007/s00227-017-3117-x 　

    ISSN： 0025-3162

    eISSN： 1432-1793

  28. Reconstructing the Invasion History of the Asian shorecrab, Hemigrapsus sanguineus (De Haan 1835) in the Western Atlantic Peer-reviewed

    April M. H. Blakeslee, Yumi Kamakukra, Jaclyn Onufrey, Wataru Makino, Jotaro Urabe, Susan Park, Carolyn L. Keogh, A. Whitman Miller, Mark S. Minton, James T. Carlton, Osamu Miura

    MARINE BIOLOGY　164　(3)　2017/03

    DOI： 10.1007/s00227-017-3069-1 　

    ISSN： 0025-3162

    eISSN： 1432-1793

  29. Microsatellite records for volume 9, issue 1 Peer-reviewed

    Ana Castillo-Paez, Axayacatl Rocha-Olivares, Celia Isabel Bisbal-Pardo, Dongfa Zhu, Dongmei Zhu, Hajime Itoh, Hiroaki Fukumori, Joichiro Koseki, John Carlos Garza, Jotaro Urabe, Yefeng Lv, Masayuki Maki, Miguel Angel del Rio-Portilla, Satoshi Chiba, Wataru Makino, Weimin Wang, Wen Song, Xuejun Chai, Yasunori Kano, Yuebin Wang, Zehui Hu

    CONSERVATION GENETICS RESOURCES　9　(1)　165-171　2017/03

    DOI： 10.1007/s12686-017-0689-1 　

    ISSN： 1877-7252

    eISSN： 1877-7260

  30. Ecological and genetic impact of the 2011 Tohoku Earthquake Tsunami on intertidal mud snails Peer-reviewed

    Osamu Miura, Gen Kanaya, Shizuko Nakai, Hajime Itoh, Satoshi Chiba, Wataru Makino, Tomohiro Nishimura, Shigeaki Kojima, Jotaro Urabe

    SCIENTIFIC REPORTS　7　2017/03

    DOI： 10.1038/srep44375 　

    ISSN： 2045-2322

  31. Variations in lignin-derived phenols in sediments of Japanese lakes over the last century and their relation to watershed vegetation Peer-reviewed

    Fujio Hyodo, Michinobu Kuwae, Naoko Sasaki, Ryoma Hayashi, Wataru Makino, Soichiro Kusaka, Narumi K. Tsugeki, Seiji Ishida, Hajime Ohtsuki, Kohei Omoto, Jotaro Urabe

    ORGANIC GEOCHEMISTRY　103　125-135　2017/01

    DOI： 10.1016/j.orggeochem.2016.11.001 　

    ISSN： 0146-6380

  32. Characterization of 22 polymorphic microsatellite loci for Cryptoblepharus nigropunctatus. Peer-reviewed

    Koseki J, Makino W, Maki M, Urabe J, Chiba S

    Conservation Genetics Resources　9　(1)　2016

  33. Ecological responses of macrobenthic communities in tidal flats to disturbances by the Great East Japan Earthquake Peer-reviewed

    Tatsuki Nishita, Wataru Makino, Takao Suzuki, Jotaro Urabe

    Ecological Impacts of Tsunamis on Coastal Ecosystems　2016

    DOI： 10.1007/978-4-431-56448-5_11 　

  34. Invasion and molecular evolution of Daphnia pulex in Japan Peer-reviewed

    Mika So, Hajime Ohtsuki, Wataru Makino, Seiji Ishida, Hitoshi Kumagai, Kenyu G. Yamaki, Jotaro Urabe

    LIMNOLOGY AND OCEANOGRAPHY　60　(4)　1129-1138　2015/07

    DOI： 10.1002/lno.10087 　

    ISSN： 0024-3590

    eISSN： 1939-5590

  35. Usefulness of the size of dark spots on the body surface as a diagnostic character distinguishing two morphologically similar Hemigrapsus species (Decapoda: Brachyura: Varunidae) Peer-reviewed

    Wataru Makino, Kotaro Kan, Masanori Sato, Yasuo Mukai, Felix Kaiser, Tatsuya Katsube, Takao Suzuki, Jotaro Urabe

    Plankton and Benthos Research　10　(1)　45-54　2015/03/20

    Publisher: Plankton Society of Japan

    DOI： 10.3800/pbr.10.45 　

    ISSN： 1882-627X 1880-8247

  36. Historical Changes in the Ecosystem Condition of a Small Mountain Lake over the Past 60 Years as Revealed by Plankton Remains and Daphnia Ephippial Carapaces Stored in Lake Sediments Peer-reviewed

    Hajime Ohtsuki, Tamotsu Awano, Narumi K. Tsugeki, Seiji Ishida, Hirotaka Oda, Wataru Makino, Jotaro Urabe

    PLOS ONE　10　(3)　e0119767　2015/03

    DOI： 10.1371/journal.pone.0119767 　

    ISSN： 1932-6203

  37. 山形県畑谷大沼堆積物の花粉分析に基づく過去６０年間の植生とスギ花粉年間推定量の変化. Peer-reviewed

    林 竜馬, 槻木玲美, 小田寛貴, 大槻 朝, 粟野 将, 牧野 渡, 占部城太郎

    日本花粉学会誌　60　(1)　13-25　2014/10

    Publisher: Palynological Society of Japan

    DOI： 10.24524/jjpal.60.1_13 　

    ISSN： 0387-1851

    More details Close

    Changes in vegetation and pollen accumulation rate (PAR) of Cryptomeria japonica during the past 60 years were reconstructed based on pollen analysis of a core from Hataya-onuma dated by Pb-210 and Cs-137 methods. Results of our study show that vegetation around Hataya-onuma has been stable since 1945. Secondary forests mainly consisting of deciduous oaks and an artificial forest of C. japonica and Pinus densiflora introduced over 60 years ago. The PAR for C. japonica in the Hataya-onuma core was relatively low (15 - 20×10^3 grains・cm^<-2>・year^<-1>) from the 1950's until the 1970's. From 1980, the PAR showed a rapid increase, reaching 40×10^3 grains・cm^<-2>・year^<-1> by the late 1990's. This trend towards an increasing PAR for the Hataya-onuma core synchronizes with that estimated for a mature forest area of C. japonica in Yamagata prefecture. The trend is also similar to that of the PAR for the Lake Biwa sediment. However, the PAR in Hataya-onuma is three-times higher than that for Lake Biwa which corresonds with the much smaller basin size of Hataya-onuma.

  38. 動物プランクトン調査

    占部城太郎, 牧野渡

    モニタリングサイト1000 陸水域調査 湖沼・湿原　17-20　2014/10

  39. Ecological impacts of the 2011 Tohoku Earthquake Tsunami on aquatic animals in rice paddies Peer-reviewed

    Yasuo Mukai, Tomoyo Suzuki, Wataru Makino, Tsubasa Iwabuchi, Mika So, Jotaro Urabe

    LIMNOLOGY　15　(3)　201-211　2014/08

    DOI： 10.1007/s10201-014-0432-5 　

    ISSN： 1439-8621

    eISSN： 1439-863X

  40. Characterization and cross-species utility of polymorphic microsatellite markers in the Asian mud snails Batillaria attramentaria and B-multiformis Peer-reviewed

    Osamu Miura, Wataru Makino, Jotaro Urabe

    CONSERVATION GENETICS RESOURCES　6　(2)　461-463　2014/06

    DOI： 10.1007/s12686-013-0126-z 　

    ISSN： 1877-7252

    eISSN： 1877-7260

  41. Immediate Ecological Impacts of the 2011 Tohoku Earthquake Tsunami on Intertidal Flat Communities Peer-reviewed

    Jotaro Urabe, Takao Suzuki, Tatsuki Nishita, Wataru Makino

    PLOS ONE　8　(5)　e62779　2013/05

    DOI： 10.1371/journal.pone.0062779 　

    ISSN： 1932-6203

  42. Annual variation in biomass and the community structure of crustacean zooplankton over 5 years in Lake Toya, Japan Peer-reviewed

    Syuhei Ban, Wataru Makino, Hiroyuki Sakano, Hiroyuki Haruna, Hiroshi Ueda

    Limnology　14　(1)　59-70　2013/01

    DOI： 10.1007/s10201-012-0387-3 　

    ISSN： 1439-8621

  43. DNA extraction and amplification methods for ephippial cases of Daphnia resting eggs in lake sediments: a novel approach for reconstructing zooplankton population structure from the past Peer-reviewed

    Seiji Ishida, Hajime Ohtsuki, Tamotsu Awano, Narumi K. Tsugeki, Wataru Makino, Yoshihisa Suyama, Jotaro Urabe

    LIMNOLOGY　13　(3)　261-267　2012/12

    DOI： 10.1007/s10201-012-0380-x 　

    ISSN： 1439-8621

    eISSN： 1439-863X

  44. Shifts in morphology and diet of non-native sticklebacks introduced into Japanese crater lakes Peer-reviewed

    Tatsuya Adachi, Asano Ishikawa, Seiichi Mori, Wataru Makino, Manabu Kume, Masakado Kawata, Jun Kitano

    ECOLOGY AND EVOLUTION　2　(6)　1083-1098　2012/06

    DOI： 10.1002/ece3.234 　

    ISSN： 2045-7758

  45. Stoichiometric effects of warming on herbivore growth: experimental test with plankters Peer-reviewed

    Wataru Makino, Qi Gong, Jotaro Urabe

    ECOSPHERE　2　(7)　Article79　2011/07

    DOI： 10.1890/ES11-00178.1 　

    ISSN： 2150-8925

  46. The long-term consequences of hybridization between the two Daphnia species, D. galeata and D. dentifera, in mature habitats Peer-reviewed

    Seiji Ishida, Akiko Takahashi, Noe Matsushima, Jun Yokoyama, Wataru Makino, Jotaro Urabe, Masakado Kawata

    BMC EVOLUTIONARY BIOLOGY　11　209　2011/07

    DOI： 10.1186/1471-2148-11-209 　

    ISSN： 1471-2148

  47. Effects of Protoceratium reticulatum yessotoxin on feeding rates of Acartia hudsonica: A bioassay using artificial particles coated with purified toxin Peer-reviewed

    Wataru Makino, Kotaro Ito, Yasukatsu Oshima, Jotaro Urabe

    HARMFUL ALGAE　7　(5)　639-645　2008/08

    DOI： 10.1016/j.hal.2008.01.002 　

    ISSN： 1568-9883

  48. Temperature affects stoichiometry and biochemical composition of Escherichia coli Peer-reviewed

    James B. Cotner, Wataru Makino, Bopaiah A. Biddanda

    MICROBIAL ECOLOGY　52　(1)　26-33　2006/07

    DOI： 10.1007/s00248-006-9040-1 　

    ISSN： 0095-3628

  49. Genotype x environment interactions, stoichiometric food quality effects, and clonal coexistence in Daphnia pulex Peer-reviewed

    LJ Weider, W Makino, K Acharya, KL Glenn, M Kyle, J Urabe, JJ Elser

    OECOLOGIA　143　(4)　537-547　2005/05

    DOI： 10.1007/s00442-005-0003-x 　

    ISSN： 0029-8549

  50. Elemental stoichiometry of a heterotrophic bacterial community in a freshwater lake: implications for growth- and resource-dependent variations Peer-reviewed

    W Makino, JB Cotner

    AQUATIC MICROBIAL ECOLOGY　34　(1)　33-41　2004/01

    DOI： 10.3354/ame034033 　

    ISSN： 0948-3055

    eISSN： 1616-1564

  51. Organic carbon biogeochemistry of Lake Superior Peer-reviewed

    James B. Cotner, Bopaiah A. Biddanda, Wataru Makino, Edward Stets

    Aquatic Ecosystem Health and Management　7　(4)　451-464　2004

    DOI： 10.1080/14634980490513292 　

    ISSN： 1463-4988

  52. The dynamics of microbial and herbivorous food webs in a coastal sea with special reference to intermittent nutrient supply from bottom intrusion Peer-reviewed

    S Nakano, Y Tomaru, T Katano, A Kaneda, W Makino, Y Nishibe, M Hirose, M Onji, S Kitamura, H Takeoka

    AQUATIC ECOLOGY　38　(4)　485-493　2004

    DOI： 10.1007/s10452-004-0441-2 　

    ISSN： 1386-2588

    eISSN： 1573-5125

  53. Growth rate-stoichiometry couplings in diverse biota Peer-reviewed

    JJ Elser, K Acharya, M Kyle, J Cotner, W Makino, T Markow, T Watts, S Hobbie, W Fagan, J Schade, J Hood, RW Sterner

    ECOLOGY LETTERS　6　(10)　936-943　2003/10

    DOI： 10.1046/j.1461-0248.2003.00518.x 　

    ISSN： 1461-023X

  54. Stay cool: habitat selection of a cyclopoid copepod in a north temperate oligotrophic lake Peer-reviewed

    W Makino, T Yoshida, H Sakano, SH Ban

    FRESHWATER BIOLOGY　48　(9)　1551-1562　2003/09

    DOI： 10.1046/j.1365-2427.2003.01108.x 　

    ISSN： 0046-5070

  55. Biological productivity of Lake Towada, a north temperate, oligotrophic, kokanee fishery lake Peer-reviewed

    W Makino, H Mikami, N Katano, M Nakagawa, N Takamura

    LIMNOLOGY　4　(2)　79-90　2003/08

    DOI： 10.1007/s10201-003-0093-2 　

    ISSN： 1439-8621

  56. Fecal pellet production between molts in a cyclopoid copepod: patterns, individual variability and implications for growth and development Peer-reviewed

    W Makino, SH Ban

    HYDROBIOLOGIA　501　(1-3)　101-107　2003/07

    DOI： 10.1023/A:1026211520190 　

    ISSN： 0018-8158

  57. Temperature and the chemical composition of poikilothermic organisms Peer-reviewed

    HA Woods, W Makino, JB Cotner, SE Hobbie, JF Harrison, K Acharya, JJ Elser

    FUNCTIONAL ECOLOGY　17　(2)　237-245　2003/04

    DOI： 10.1046/j.1365-2435.2003.00724.x 　

    ISSN： 0269-8463

  58. Bacterial and algal resource limitation and the microbial food web: A test of the light:nutrient hypothesis. Peer-reviewed

    Elser J, Kyle M, Makino W, Yoshida T, Urabe J

    Aquatic Microbial Ecology　31　49-65　2003/04

  59. Are bacteria more like plants or animals? Growth rate and resource dependence of bacterial C : N : P stoichiometry Peer-reviewed

    W Makino, JB Cotner, RW Sterner, JJ Elser

    FUNCTIONAL ECOLOGY　17　(1)　121-130　2003/02

    DOI： 10.1046/j.1365-2435.2003.00712.x 　

    ISSN： 0269-8463

    eISSN： 1365-2435

  60. Ecological stoichiometry in the microbial food web: a test of the light : nutrient hypothesis Peer-reviewed

    JJ Elser, M Kyle, W Makino, T Yoshida, J Urabe

    AQUATIC MICROBIAL ECOLOGY　31　(1)　49-65　2003/02

    ISSN： 0948-3055

    eISSN： 1616-1564

  61. Reduced light increases herbivore production due to stoichiometric effects of light/nutrient balance Peer-reviewed

    J Urabe, M Kyle, W Makino, T Yoshida, T Andersen, JJ Elser

    ECOLOGY　83　(3)　619-627　2002/03

    ISSN： 0012-9658

  62. Evidence of phosphorus-limited individual and population growth of Daphnia in a Canadian Shield lake Peer-reviewed

    W Makino, J Urabe, JJ Elser, C Yoshimizu

    OIKOS　96　(2)　197-205　2002/02

    DOI： 10.1034/j.1600-0706.2002.960201.x 　

    ISSN： 0030-1299

  63. Food quality determinants for Daphnia growth in P-limited lakes Peer-reviewed

    J Urabe, Makino, W, K Hayakawa, JJ Elser

    INTERNATIONAL ASSOCIATION OF THEORETICAL AND APPLIED LIMNOLOGY, VOL 28, PT 2, PROCEEDINGS　28　1089-1094　2002

    ISSN： 0368-0770

  64. ユスリカ蛹の浮上は魚の補食からミジンコを解放し間接的に透明度を向上させたか？

    牧野 渡, 加藤秀男, 高村典子, 水谷 寿, 片野 登, 三上 一

    国立環境研究所研究報告　167　15-25　2001/10

    More details Close

    Hydrobiologia 442: 309-317 (2001)に加筆修正

  65. 十和田湖における甲殻類動物プランクトン生産量の試算.

    牧野 渡

    国立環境研究所研究報告　167　26-30　2001/10

  66. Did chironomid emergence release Daphnia from fish predation and lead to a Daphnia-driven clear-water phase in Lake Towada, Japan? Peer-reviewed

    W Makino, H Kato, N Takamura, H Mizutani, N Katano, H Mikami

    HYDROBIOLOGIA　442　(1-3)　309-317　2001/01

    ISSN： 0018-8158

  67. Response of life history traits to food conditions in a cyclopoid copepod from an oligotrophic environment Peer-reviewed

    W Makino, SH Ban

    LIMNOLOGY AND OCEANOGRAPHY　45　(2)　396-407　2000/03

    ISSN： 0024-3590

  68. 十和田湖における動物プランクトン群集の季節変化 - 1998年の結果から.

    牧野 渡

    国立環境研究所研究報告　146　41-48　1999/10

  69. 十和田湖における動物プランクトン計数データ（1998年） .

    牧野 渡

    国立環境研究所研究報告　146　190-202　1999/10

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    資料

  70. Diel changes in vertical overlap between Cyclops strenuus (Copepoda; Cyclopoida) and its prey in oligotrophic Lake Toya, Hokkaido, Japan Peer-reviewed

    W Makino, S Ban

    JOURNAL OF MARINE SYSTEMS　15　(1-4)　139-148　1998/06

    DOI： 10.1016/S0924-7963(97)00073-0 　

    ISSN： 0924-7963

  71. 洞爺湖の動物プランクトン動態と漁業生産

    伴 修平, 牧野 渡, 坂野博之, 春名寛幸, 上田 宏

    陸水学雑誌　58　89-91　1997/10

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    シンポジウム課題講演報告

  72. 今,なぜ貧栄養湖研究なのか? 貧栄養湖研究の現状と未来

    日野 修次, 三上 英敏, 石川 靖, 坂田 康一, 有末 二郎, 今田 和史, 安富 亮平, 伴 修平, 牧野 渡, 坂野 博之, 春名 寛幸, 上田 宏, 吉岡 崇仁, 李 在鎔, 河合 崇欣, 岩熊 敏夫, 中里 亮治, 沖野 外輝夫, 坂本 充, 高村 典子, 手塚 泰彦, 占部 城太郎, 安野 正之

    陸水學雜誌　58　(1)　83-104　1997/03/01

    Publisher: The Japanese Society of Limnology

    DOI： 10.3739/rikusui.58.83 　

    ISSN： 0021-5104

  73. Diel vertical migration and feeding rhythm of Daphnia longispina and Bosmina coregoni in Lake Toya, Hokkaido, Japan. Peer-reviewed

    Makino, W, H. Haruna, S. Ban

    Hydrobiologia　337　133-143　1996/10

    DOI： 10.1007/BF00028514 　

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Misc. 3

  1. 飯綱高原の池に出現した橈脚類および枝角類動物プランクトン

    牧野渡

    第１回 親子自然教室「水の中の世界」報告書　12-18　2019/02

  2. 東日本大震災後の干潟生物群集の形成過程：その人為的影響と生態系機能の活用

    占部城太郎, 鈴木孝男, 牧野渡, 大槻朝, 金谷弦, 中川雅博, 竹本徳子, 秦範子, 柚原剛

    三井物産環境基金2015-2017年度最終報告書　1-27　2018/03

  3. 遺伝子情報に基づく淡水橈脚類の種判別とその応用

    牧野渡

    日本プランクトン学会報　62　(1)　16-21　2015

    Publisher: 日本プランクトン学会

    ISSN： 0387-8961

    More details Close

    http://iss.ndl.go.jp/books/R000000004-I026281773-00

Books and Other Publications 3

  1. 占部城太郎（編）湖沼近過去調査法 よりよい湖沼環境と保全目標設定のために

    牧野渡

    2014/12/25

  2. シリーズ現代の生態学９ 淡水生態学のフロンティア

    石田聖二, 牧野渡

    2012/03/25

  3. rikusui no jiten

    Makino, Wataru

    Kodansha　2006/03/31

Presentations 34

  1. 日本産オカメミジンコ属の多様性と分布：DNAバーコーディングの結果から

    牧野渡, 鈴木碩通, 大竹裕里恵, 占部城太郎, 辻彰洋

    日本生態学会第72回全国大会　2025/03

  2. 実は1990年代に生じていた北米原産マルミジンコの琵琶湖北湖への侵入

    牧野渡, 槻木玲美, 占部城太郎, 中野伸一

    日本生態学会第71回全国大会　2024/03

  3. 日本産ネコゼミジンコ属の多様性と分布：DNAバーコーディングの結果から

    牧野渡, 鈴木碩通, 大竹裕里恵, 占部城太郎

    日本生態学会第70回全国大会　2023/03

  4. 日本産ミジンコ属の多様性と分布：DNAバーコーディングの結果から

    牧野渡, 大槻朝, 丸岡奈津美, 占部 城太郎

    日本生態学会第69回全国大会　2022/03

  5. Temporal β-diversity reveals the temporal scale difference in factors regulating zooplankton community structures: an example in a small lake

    Ishara Uhanie PERERA, Natsumi MARUOKA, Wataru MAKINO, Jotaro URABE

    2021/03/18

  6. Untangling lineages of freshwater copepods having a broad geographical range: The cases of Cyclops vicinus and C. kikuchii.

    Imane SIOUD, Wataru MAKINO, Jotaro URABE

    2021/03/18

  7. 淡水産微小甲殻類の多様性と生態 Invited

    牧野渡

    日本微生物生態学会第33回大会　2019/09/11

  8. 自然栽培水田は表在性ミジンコ類の代替生息地として機能する

    牧野渡, 西川潮

    日本生態学会第66回大会　2019/03/18

  9. 湖沼の動物プランクトン群集組成を超並列DNAシーケンスで調べる前に解決すべき問題点について Invited

    牧野渡

    国立環境研究所琵琶湖分室セミナー 第21回　2019/02/19

  10. 動物プランクトンの DNA バーコーディングとその応用

    牧野渡, 中川惠, 永田貴丸, 高村典子

    日本陸水学会第83回大会　2018/10/08

  11. タマミジンコ（Moina属）の「正しい」種判別とその応用

    牧野渡

    日本生態学会第65回大会　2018/03/17

  12. 日本淡水産動物プランクトンのDNAバーコーディング（経過報告）

    日本プランクトン学会・日本ベントス学会 合同大会　2017/09/03

  13. DNAバーコードを用いた日本淡水動物プランクトンの種判別

    牧野渡, 丸岡奈津美, 中川惠, 高村典子

    日本生態学会第64回大会　2017/03/16

  14. MIG-seqを用いた集団遺伝学的解析：動物プランクトンへの適用

    牧野渡, 松木悠, 陶山佳久, 占部城太郎

    第63回日本生態学会大会　2016/03/24

  15. 日本のホロミジンコ：その正体を探る

    山元綾弥香, 牧野渡, 占部城太郎

    第63回日本生態学会大会　2016/03/24

  16. DNA barcoding of Japanese freshwater calanoid copepods International-presentation

    Wataru Makino, Jotaro Urabe

    Unraveling biodiversity from DNA – from the management of databases to the use of next generation sequencers –　2014/09/19

  17. 遺伝子情報に基づく淡水カイアシ類の種判別とその応用 Invited

    2014年度日本プランクトン学会春季シンポジウム「遺伝子解析とプランクトン研究」　2014/03/26

  18. 欧州沿岸域における外来種タカノケフサイソガニの由来

    牧野渡, Felix Kaiser, 勝部達也, 鈴木孝男, 占部城太郎

    第61回日本生態学会大会　2014/03/17

  19. タカノケフサイソガニの遺伝的変異と地理的変異

    牧野渡, Felix Kaiser, 勝部達也, 鈴木孝男, 占部城太郎

    日本ベントス学会・日本プランクトン学会 合同大会　2013/09/28

  20. 動物プランクトン情報を用いた湖沼生物群集の復元

    牧野渡, 大槻朝, 石田聖二, 占部城太郎

    第60回日本生態学会大会　2013/03/06

  21. MAINTENANCE OF PARAPATRY IN FRESHWATER COPEPODS International-presentation

    2012 ASLO Aquatic Sciences Meeting　2012/07/13

  22. Finding copepod footprints: DNA barcoding for the resting eggs in lake sediments

    Makino W, Urabe J

    第59回日本生態学会大会　2012/03/21

  23. 隠蔽種の共存・非共存と繁殖干渉

    第57回日本生態学会大会　2010/03/17

  24. Phylogeography of Japanese freshwater copepods as background information on the potential impacts of warming on zooplankton fauna International-presentation

    The Symposium of the International Society of Limnology (SIL) 2009　2009/10

  25. ヒゲナガケンミジンコmtCOI塩基配列の「中途半端な」種内変異

    第56回日本生態学会大会　2009/03

  26. 日本におけるSinodiaptomus属ケンミジンコの分布と遺伝的分化

    第73回日本陸水学会大会　2008/10

  27. 移動分散に果たすニッチ幅の役割：浮遊性ヒゲナガケンミジンコを例として

    占部 城太郎

    第55回日本生態学会大会　2008/03

  28. ヒゲナガケンミジンコの個体群遺伝構造と移動分散

    第54回日本生態学会大会　2007/03

  29. 日本産ヤマヒゲナガケンミジンコは隠蔽種の複合体? DNAバーコーディングの結果から

    第71回日本陸水学会大会　2006/09

  30. ヤマヒゲナガケンミジンコの遺伝構造の地理的変異

    第53回日本生態学会大会　2006/03

  31. Mitochondrial gene diversity of Acanthodiaptomus pacificus in Japan International-presentation

    2nd Scientific Congress of East Asia Federation of Ecological Societies　2006/03

  32. 植物プランクトンの化学組成（炭素：リン比）がケンミジンコの成長と再生産に与える効果

    第52回日本生態学会大会　2005/03

  33. 宮城県のSinodiaptomus valkanoviについて

    日本ベントス学会・日本プランクトン学会 合同大会　2004/09

  34. Increased body size of a copepod due to fish predation? International-presentation

    H. Haruna, S. Ban

    SIL XXIX Congress　2004/08

Show all Show first 5

Research Projects 12

  1. プランクトンAI画像判別計数システムによる湖沼・ダム湖生態系監視手法の確立と展開

    占部 城太郎, KASS Jamie, 藤原 直樹, 一瀬 諭, 安達 孝実, 辻 彰洋, 牧野 渡, 伊藤 康一, 青木 孝文, 樋村 正雄, 杉島 英樹, 堀江 啓史, 吉成 暁, 阿部 真己, 高橋 巧

    Offer Organization: 独立行政法人環境再生保全機構

    System: 環境研究総合推進費

    2024/04 - 2027/03

  2. Ecology, genetics, and beta diversity of zooplankton in "managed floodplain" rice paddies

    Makino Wataru

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2018/04/01 - 2021/03/31

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    In this study, we 1) examined the possibility that the copy number of ribosomal RNA genes is higher in microcrustaceans in rice paddies than in microcrustaceans in lakes and reservoirs where the period of inundation is relatively long, using the quantitative PCR method, but the results did not support the hypothesis. Furthermore, 2) the possibility that the zooplankton diversity of the entire region increases when there is a mixture of "rice paddies" that differ greatly in the timing of inundation was examined, and the results supported this hypothesis. However, paddy fields that were flooded later in the year had more paddy weeds than those that were not, and the diversity increased as a result of the emergence of microcrustaceans that used the paddy weeds as a substrate. Therefore, it was not possible to distinguish the effect of inundation timing from the effect of paddy weeds.

  3. Establishment and application to lake ecosystem of next generation monitoring system

    Takamura Noriko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: National Institute for Environmental Studies

    2015/04/01 - 2019/03/31

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    We developed a reference database for DNA metabarcoding analyses (MA) in Lake Kasumigaura. We also examined the relationships between the number of DNA sequences of target plankton taxa, and their abundance, and found significant positive correlations between them. This suggests eDNA MA is applicable to quantitative assessments for the target organisms. By using the reference database, we conducted a high-resolution gut content analysis of a commercially important planktivorous fish species and revealed that its dietary interactions with the prey organisms were highly dynamic seasonally. Further, we demonstrated the eDNA MA is useful to quantify the temporal dynamics of the whole planktonic community. We also applied a convergent cross-mapping to long-term monitoring data elucidating that bottom-up linkages between nutrients, primary production, and, rotifers and cyclopoids may be a key mechanism supporting high planktivorous fish abundance in hypereutrophic lakes.

  4. Effects of farm-pond and paddy-field constructions on the diversity of aquatic microcrustaceans

    Makino Wataru

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    In Japan, the effects of farm-pond and paddy-field constructions on the diversity of aquatic microcrustaceans remain unclear. By investigating the geographic structures of genetic compositions in farm-pond and paddy-field zooplankton, this study tried to approve the hypothesis that farm-pond and paddy-field constructions have increased the population sizes of zooplankters there, which had been distributed in floodplains before such constructions. The obtained results in the genetic analysis, including the genotyping by sequencing, of the genus Diaphanosoma and the genus Moina in Japan, suggested that farm-pond and paddy-field dwelling species at present have higher dispersal abilities than those in their counterparts. It is therefore argued that historical farm-pond and paddy-field constructions have functioned to stabilize/increase the population size of animals that had been distributed in floodplains prior to the construction of farm-pond and paddy-field.

  5. Functional roles of reproductive mode in persisting population and maintaining genetic diversity of daphnia species.

    Urabe Jotaro, MAKINO WATARU

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

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    In general, Daphnia reproduce parthenogenetically but switch to sexual reproduction for producing resting eggs that are capable of overwintering. If certain parthenogenetic individuals are competitively superior to others, their success results to reduce genetic diversity. However, it not yet clear how competitive persistence of daphnia populations are related with maintenance of the genetic diversities. This study showed that some of competitively inferior individuals are highly sensitive to environmental changes in initiating production of the resting or sexual eggs By producing these eggs before intensifying the competitive interactions, daphnia species maintained their genetic diversities.

  6. Conservation palaeontology: protecting future biodiversity by past information

    CHIBA Satoshi, MAKINO Wataru

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    Temporal changes in environmental condition and its impacts on community were estimated by investigating fossil and modern land snail assemblages on the Ogasawara and Ryukyu Islands. The results of stable isotope analyses and morphological analyses suggest that habitat shift from wet to dry condition occurred at the end of the last ice age (ca. 10ka), and it caused sSerious extinction and rapid changes of species composition. On the Ogasawara Islands, extinction of land snails occurred due to large changes in vegetation after 300 years ago when first human immigration occurred. These suggest that habitat of these islands were formerly more moist than the present condition, and land snail species adapted to wet habitat were domminated in the community in the past. These information would be crucial for conservation plan for these snails.

  7. Ecological stoichiometry for explaining habitat segregations in freshwater copepods

    MAKINO Wataru

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2011 - 2013

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    The effects of algal phosphorus content on the development of freshwater calanoid copepods were investigated for two groups of species (lowland and highland groups). Using chemostats, the cellular C:P ratio of a cryptomonad species was manipulated, and the excess amount of the alga was supplied to copepods, whose postembryonic development was observed individually. With decreases in algal phosphorus content the development of copepods were severely retarded in both of the groups. It was also revealed that the survivorship of individuals in lowland groups was low when temperature was low. Thus the relative importance of temperature may be stronger than that of algal phosphorus content for habitat separations observed between the two groups of copepods.

  8. Mitigation of stoichiometry effects on biological interactions by biodiversity.

    URABE Jotaro, MMAKINO Wataru, ISHIDA Seiji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2010 - 2012

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    This study was performed to clarify experimentally how stoichiometric impacts by putative environmental changes on prey (algae) - consumer (Daphnia) interactions are altered depending on genetic and species identities of consumers. Theoretical and experimental analyses showed that optimal feeding rates of the consumers change depending not only on abundance but also stoichiometry of algal food, that threshold food levels of the consumers change even within species according to the genotypes, and that outcomes of competitive interactions change depending on species identities of the consumers. These results support an idea that stoichiometric impacts on ecosystem processes are different among communities with different genetic and species identities.

  9. Population genetic structure of freshwater zooplankton in Japan

    MAKINO Wataru

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2007 - 2008

  10. MECHANISMS OF ADAPTIVE DIVERGENCES

    CHIBA Satoshi, MAKINO Wataru, YAMAZAKI Kazuhito, TOYOFUKU Takashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2006 - 2008

  11. 「コスモポリタン的」動物プランクトンの生活史特性と遺伝子組成の湖沼間比較

    牧野 渡

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 若手研究(B)

    Institution: 東北大学

    2004 - 2006

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    1)「コスモポリタン的」な分布を示すカイアシ類として本研究ではAcanthodiaptomus pacificus(ヤマヒゲナガケンミジンコ)に焦点を置いてきたが、今年度はその対照種としてEodiaptomus japonicus(ヤマトヒゲナガケンミジンコ)を取りあげて遺伝子解析に供した。ヤマヒゲナガケンミジンコが様々なタイプのハビタットに生息したのに対し、ヤマトヒゲナガケンミジンコのほとんどは、低地(標高1000m以下)のため池に出現した。なお両種の共存は、本研究では認められなかった。 2)ヤマトヒゲナガケンミジンコにおいても、ヤマヒゲナガケンミジンコと同様にミトコンドリアDNA・COI領域の630bpが遺伝マーカーとして使用可能であることが分った。得られた配列データを元に近隣接合法(K2Pモデル)にて系統樹を作成した結果、ヤマトヒゲナガケンミジンコの遺伝的分化(K2P距離で最大2.6%)は、ヤマヒゲナガケンミジンコ(K2P距離で最大20%)ほど顕著ではなく、DNAバーコーディング的に判断するなら、ヤマトヒゲナガケンミジンコは一種類であるとみなしてよいことがわかった。 3)いずれの種類とも、個体群遺伝構造の大半が個体群間での変異で説明できたが、「コスモポリタン的」なヤマヒゲナガケンミジンコでは、その傾向がヤマトヒゲナガケンミジンコより強かった(個体群間変異の寄与率は、ヤマヒゲナガケンミジンコでは90%、ヤマトヒゲナガケンミジンコでは50%)。これはヤマヒゲナガケンミジンコの潜在的な生息環境が多様である(ニコスモポリタン的である)ことと関係があると考えられた。すなわちヤマヒゲナガケンミジンコにとっては、移動分散先の物理化学あるいは生物的環境が現在のそれと顕著に異なる可能性が高いので、移動分散先で迅速に局所適応する能力を獲得したのであろう。そのため移動分散先では先住者の遺伝子型が卓越するため個体郡内での遺伝的変異が小さくなる。この創始者効果が以後長期間にわたり維持されているため、個体群遺伝構造に対する個体群間変異の寄与率が高いのだろうと考えられた。

  12. 植食性動物プランクトンにおけるファシリテーション効果

    牧野 渡

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 特別研究員奨励費

    2002 - 2003

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    平成15年度は、ミジンコ(Daphnia)と同じ植食性動物プランクトンでありながら、ミジンコよりも遥かに飼育が困難であることが前年度に発覚したヒゲナガケンミジンコ類の飼育体系の確立を目指してスタートした。平成15年9月までは琵琶湖からEodiaptomusを採集し、平成15年10月以降は仙台市近郊のため池からAcanthodiaptomus、Eodiaptomus、およびSinodiaptomusを採集し、それぞれミジンコの餌として用いていた植物プランクトンのScenedesmus(緑藻の一種)を与えてみたが、この方法だと、餌を潤沢に与えても発育途上で死滅することがわかった。これはScenedesmusがヒゲナガケンミジンコにとって好ましくない(必須化学成分の欠如などの理由が考えられる)ためと考え、打開策としてScenedesmus以外の藻類株を各地の研究機関から購入し、それを上記三種に与えてみた。新たに試した藻類は、珪藻のCyclotella、クリプト藻のCryptomonas、および緑藻のChlamydomonasである。これらの培養系を確立したのち、いずれも良質な細胞を餌として充分量与えたのだが、CyclotellaとChlamydomonasはヒゲナガケンミジンコ類は発育途上で死滅した。一方、Cryptomonasを与えたヒゲナガケンミジンコは無事に成熟することがわかった。ただし、産生された卵の孵化率には種間差があり、卵がほぼ100%孵化するのはSinodiaptomusだけで、ほかの種類ではその値が10-20%と著しく小さいことがわかった。なお福島県以北でのSinodiaptomusの分布は知られておらず、本研究がその初報告となる。

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Teaching Experience 8

  1. 東北大学理学部生物学科 動物生態学実習（流域生態分野）

  2. 東北大学全学教育 自然科学総合実験 課題11 DNAによる生物の識別

  3. 東北大学理学部生物学科 ⽣態・進化⽣物学特選科⽬I

  4. 仙台高等専門学校（非常勤講師）生物学

  5. 東北大学理学部生物学科 生物学演習I

  6. 東北大学全学教育 自然科学総合実験 課題10 細胞

  7. 東北大学理学部生物学科 生物学へのアプローチ

  8. 東北大学理学部生物学科 生態学実習

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Social Activities 1

  1. 東日本グリーン復興プロジェクト 被災した地域の生き物調査

    2012/05 - 2019/08

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    市民調査