Details of the Researcher

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Yuta Kudo
Section
Frontier Research Institute for Interdisciplinary Sciences
Job title
Associate Professor
Degree

  • 博士（農学）（東北大学）

  • 修士（農学）（東北大学）

Profile

天然物化学を専攻しています。
フグやイモリに含まれる強力な神経毒テトロドトキシンを主な研究対象として、質量分析器を用いた新規類縁体および生合成中間体の探索、単離精製、構造解析、生物活性評価をしています。有毒イモリから得られた推定生合成中間体の化学構造をもとに、陸上テトロドトキシンの生合成経路について新たな仮説を提唱しました。生合成経路の解明に向けて、メタゲノム解析を含めた生合成遺伝子の探索をしています。また、イモリの毒の起源についても研究しています。
米国カリフォルニア大学サンディエゴ校(UCSD) スクリプス海洋研究所Moore研究室では海洋放線菌が生産する抗マラリア活性化合物とシグナル分子の生合成やヒト虫歯菌が生産する抗生物質について研究しました。現在も放線菌に関する研究を行っており、特に放線菌シグナル分子の化学-酵素合成法および迅速同定法の構築に注力しています。

Research History 12

  • 2024/04 - Present
    Tohoku University　Graduate School of Agricultural Science

  • 2024/04 - Present
    Tohoku University　Frontier Research Institute for Interdisciplinary Sciences Creative Interdisciplinary Research Division　Associate Professor

  • 2023/04 - Present
    Tohoku Initiative for Fostering Global Researchers for Interdisciplinary Sciences (TI-FRIS)　TI-FRIS Fellow

  • 2024/04 - 2025/03
    Osaka University　Institute for Protein Research

  • 2019/04 - 2024/03
    Tohoku University, The Frontier Research Institute for Interdisciplinary Sciences　Creative Interdisciplinary Research Division　Assistant Professor

  • 2019/06 - 2022/03
    科学技術人材育成のコンソーシアムの構築事業　第二世代 育成対象者

  • 2018/04 - 2019/03
    Tohoku University　Graduate School of Agriculture　Research Assistant Professor

  • 2016/04 - 2018/03
    JSPS　Overseas Reserch Fellow (UCSD Scripps Instituion of Oceanography)

  • 2013/04 - 2016/03
    JSPS

  • 2022/04 - Present
    連携型博士研究人材総合育成システムの構築　連携育成対象者

  • 2022/02 - 2024/03
    Tohoku University

  • 2019/04 - 2024/03
    Tohoku University　Graduate School of Agricultural Science

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Education 3

  • Tohoku University　Graduate School of Agriculture　Division of Bioscience and Biotechnology for Future Bioindustries Department of Applied Bioorganic Chemistry

    2013/04 - 2016/03

  • Tohoku University　Graduate School of Agriculture　Division of Bioscience and Biotechnology for Future Bioindustries Department of Applied Bioorganic Chemistry

    2011/04 - 2013/03

  • Tohoku University　Faculty of Agriculture

    2007/04 - 2011/03

Committee Memberships 11

  • 日本農芸化学会　和文誌編集委員会委員

    2025/03 - Present

  • 東北大学学際科学フロンティア研究所　DEI推進WG

    2024/01 - Present

  • toxin symposium　committee member

    2023/01 - Present

  • 天然物化学談話会　世話人

    2022/08 - Present

  • 東北大学オープンオンライン教育開発推進センター　講座企画運営委員会

    2024/04 - 2025/03

  • FRIS　FRIS-URO WG

    2024/04 - Present

  • 東北大学大学院農学研究科　冷凍室管理運営委員会

    2023/04 - Present

  • 日本農芸化学会東北支部会　若手の会世話人

    2023/09 - 2023/12

  • 東北大学大学院農学研究科　冷凍室管理運営委員会 委員長

    2021/04 - 2023/03

  • 日本農芸化学会　日本農芸化学会2021年度仙台大会実行委員

    2020/03 - 2021/03

  • 東北大学　サマースクール2013 実行委員長

    2013/03 - 2013/09

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Professional Memberships 7

  • 日本水産学会

    2023/09 - Present

  • 日本生薬学会

    2022/04 - Present

  • 毒素シンポジウム

    2022/04 - Present

  • American Chemical Society

    2021/07 - Present

  • マリントキシン研究会

    2021/03 - Present

  • American Society of Pharmacognosy

    2019 - Present

  • JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

    2011/12 - Present

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Research Interests 5

  • biosynthesis

  • LC-MS

  • structure determination

  • bioactive compound

  • natural product chemistry

Research Areas 1

  • Life sciences / Bioorganic chemistry / Natural product chemistry

Awards 11

  1. 日本生薬学会 第70回年会 優秀発表賞（口頭）

    2024/10　日本生薬学会　放線菌シグナル分子の迅速同定を目指したγ-ブチロラクトン化合物の化学-酵素合成法の構築

  2. Article award

    2023/03　Japan Society for Bioscience, Biotechnology, and Agrochemistry　Mass spectrometry–guided discovery of new analogs of bicyclic phosphotriester salinipostin and evaluation of their monoacylglycerol lipase inhibitory activity

  3. 日本農芸化学会東北支部奨励賞

    2022/12　日本農芸化学会東北支部　新規化合物の探索を軸とする二次代謝産物の生合成研究

  4. 毒素シンポジウム奨励賞

    2022/09　毒素シンポジウム

  5. ACS Editors' Choice

    2020/09　American Chemical Society and American Society of Pharmacognosy　Structures of N-Hydroxy-Type Tetrodotoxin Analogues and Bicyclic Guanidinium Compounds Found in Toxic Newts

  6. 東北大学 総長賞

    2016/03　東北大学

  7. 第57回天然有機化合物討論会 奨励賞(ポスター発表)

    2015/10　第57回天然有機化合物討論会　有毒イモリから得られた新規環状グアニジン化合物群とテトロドトキシン生合成経路の考察

  8. 東北大学 農学研究科 科長賞

    2016/03　東北大学

  9. 日本農芸化学会東北支部 若手奨励賞

    2015/09　日本農芸化学会東北支部

  10. 新学術領域研究「天然物ケミカルバイオロジー～分子標的と活性制御～」第7回若手研究者ワークショップ 優秀講演者

    2015/06　新学術領域研究「天然物ケミカルバイオロジー～分子標的と活性制御～」第7回若手研究者ワークショップ　生合成経路解明を目指した新規テトロドトキシン類縁体、生合成中間体の探索

  11. 平成25年度化学系学協会東北大会及び日本化学会東北支部70周年記念国際会議 優秀ポスター賞

    2013/09　平成25年度化学系学協会東北大会及び日本化学会東北支部70周年記念国際会議　The structure of the novel tetrodotoxin analog, 6-deoxytetrodotoxin, and structure activity relationship study on C-6 and C-11 hydroxyl groups of tetrodotoxin

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Papers 36

  1. Identification of γ-butyrolactone signalling molecules in diverse actinomycetes using resin-assisted isolation and chemoenzymatic synthesis

    Yuta Kudo, Keiichi Konoki, Mari Yotsu-Yamashita

    RSC Chemical Biology　6　(4)　630-641　2025

    Publisher: Royal Society of Chemistry (RSC)

    DOI： 10.1039/d5cb00007f 　

    eISSN： 2633-0679

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    γ-Butyrolactone signalling molecules were identified in diverse actinomycetes through resin-assisted isolation, and LCMS analysis using chemoenzymatically synthesized standards.

  2. Mass spectrometry–guided discovery of new analogs of bicyclic phosphotriester salinipostin and evaluation of their monoacylglycerol lipase inhibitory activity International-journal Peer-reviewed

    Yuta Kudo, Keiichi Konoki, Mari Yotsu-Yamashita

    Bioscience, Biotechnology, and Biochemistry　86　(10)　1333-1342　2022/08/02

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/bbb/zbac131 　

    eISSN： 1347-6947

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    Natural products containing the highly unusual phosphotriester ring are known to be potent serine hydrolase inhibitors. The long-chain bicyclic enol-phosphotriester salinipostins (SPTs) from the marine actinomycete Salinispora have been identified as selective antimalarial agents. A potential regulatory function has been suggested for phosphotriesters based on their structural relationship with actinomycete signaling molecules and the prevalence of spt-like biosynthetic gene clusters across actinomycetes. In this study, we established a mass spectrometry–guided screening method for phosphotriesters focusing on their characteristic fragment ions. Applying this screening method to the SPT producer Salinispora tropica CNB-440, new SPT analogs (4-6) were discovered and their structures were elucidated by spectroscopic analyses. Previously known and herein-identified SPT analogs inhibited the activity of human monoacylglycerol lipase (MAGL), a key serine hydrolase in the endocannabinoid system, in the nanomolar range. Our method could be applied to the screening of phosphotriesters, potential serine hydrolase inhibitors and signaling molecules.

  3. Identification of Tricyclic Guanidino Compounds from the Tetrodotoxin-Bearing Newt Taricha granulosa Peer-reviewed

    Yuta Kudo, Charles T. Hanifin, Mari Yotsu-Yamashita

    Organic Letters　23　(9)　3513-3517　2021/05/07

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.orglett.1c00916 　

    ISSN： 1523-7060

    eISSN： 1523-7052

  4. Expansion of Gamma-Butyrolactone Signaling Molecule Biosynthesis to Phosphotriester Natural Products Peer-reviewed

    Yuta Kudo, Takayoshi Awakawa, Yi-Ling Du, Peter A. Jordan, Kaitlin E. Creamer, Paul R. Jensen, Roger G. Linington, Katherine S. Ryan, Bradley S. Moore

    ACS Chemical Biology　15　(12)　3253-3261　2020/11/24

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acschembio.0c00824 　

    ISSN： 1554-8929

    eISSN： 1554-8937

  5. Structures of N-Hydroxy-Type Tetrodotoxin Analogues and Bicyclic Guanidinium Compounds Found in Toxic Newts Peer-reviewed

    Yuta Kudo, Charles T. Hanifin, Yuichi Kotaki, Mari Yotsu-Yamashita

    Journal of Natural Products　83　(9)　2706-2717　2020/09/08

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jnatprod.0c00623 　

    ISSN： 0163-3864

    eISSN： 1520-6025

  6. Cariogenic Streptococcus mutans Produces Tetramic Acid Strain-Specific Antibiotics That Impair Commensal Colonization Peer-reviewed

    †Xiaoyu Tang, †Yuta Kudo, †Jonathon L. Baker, Sandra LaBonte, Peter A. Jordan, Shaun M, K. McKinnie, Jian Guo, Tao Huan, Bradley S. Moore, Anna Edlund

    ACS Infectious Diseases　6　(4)　563-571　2020/04/10

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acsinfecdis.9b00365 　

    ISSN： 2373-8227

    eISSN： 2373-8227

  7. Cyclic Guanidine Compounds from Toxic Newts Support the Hypothesis that Tetrodotoxin is Derived from a Monoterpene Peer-reviewed

    Yuta Kudo, Takeshi Yasumoto, Dietrich Mebs, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　55　(30)　8728-8731　2016/07

    DOI： 10.1002/anie.201602971 　

    ISSN： 1433-7851

    eISSN： 1521-3773

  8. Total Synthesis and Structural Revision of (±)-Mauritamide B Peer-reviewed

    Ryosuke Hirozumi, Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita

    Journal of Natural Products　2025/02/21

    DOI： 10.1021/acs.jnatprod.5c00019 　

  9. Synthesis of Saxitoxin Biosynthetic Intermediates: Reveal the Mechanism for Formation of its Tricyclic Skeleton in Biosynthesis Peer-reviewed

    Ryosuke Hirozumi, Mayu Hakamada, Takashi Minowa, Yuko Cho, Yuta Kudo, Keiichi Konoki, Yasukatsu Oshima, Kazuo Nagasawa, Mari Yotsu-Yamashita

    Chemistry - An Asian Journal　2024/09

    DOI： 10.1002/asia.202400834 　

  10. Assay for okadaic acid O-acyl transferase using HPLC-FLD Peer-reviewed

    Masato Terauchi, Yuki Komazaki, Atsushi Yoshino, Yuko Cho, Yuta Kudo, Mari Yotsu-Yamashita, Keiichi Konoki

    Bioscience, Biotechnology, and Biochemistry　88　(9)　999-1006　2024/06/17

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/bbb/zbae085 　

    eISSN： 1347-6947

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    ABSTRACT Dinophysistoxin 1 (DTX1, 1) and okadaic acid (OA, 2), produced by the dinoflagellates Dinophysis spp. and Prorocentrum spp., are primary diarrhetic shellfish toxins (DSTs), which may cause gastric illness in people consuming such as bivalves. Both compounds convert to dinophysistoxin 3 (DTX3, 3; generic name for 1 and 2 with fatty acids conjugated at 7-OH) in bivalves. The enzyme okadaic acid O-acyl transferase (OOAT) is a membrane protein found in the microsomes of the digestive glands of bivalves. In this study, we established an in vitro enzymatic conversion reaction using 4-nitro-2,1,3-benzoxadiazole (NBD)-OA (4), an OA derivative conjugated with (R)-(-)-4-nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole (NBD-APy) on 1-CO2H, as a substrate. We detected the enzymatically produced 3, NBD-7-O-palmitoyl-OA (NBD-Pal-OA), using high-performance liquid chromatography-fluorescence detection. We believe that an OOAT assay using 4 will facilitate the fractionation and isolation of OOAT in the future.

  11. Synthesis and Identification of decarbamoyloxySaxitoxins in Toxic Microalgae and their Reactions with the Oxygenase, SxtT, Reveal Saxitoxin Biosynthesis Peer-reviewed

    Mayu Hakamada, Chihiro Tokairin, Hayate Ishizuka, Kanna Adachi, Toma Osawa, Shiori Aonuma, Ryosuke Hirozumi, Shigeki Tsuchiya, Yuko Cho, Yuta Kudo, Keiichi Konoki, Yasukatsu Oshima, Kazuo Nagasawa, Mari Yotsu-Yamashita

    Chemistry – A European Journal　2024/01/25

    Publisher: Wiley

    DOI： 10.1002/chem.202304238 　

    ISSN： 0947-6539

    eISSN： 1521-3765

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    Saxitoxin (STX, 1) is a representative compound of paralytic shellfish toxins (PSTs) that are produced by marine dinoflagellates and freshwater cyanobacteria. Although several pathways have been proposed for the biosynthesis of STX, the order of ring and side chain hydroxylation, and formation of the tricyclic skeleton have not been well established. In this study, 12,12‐dideoxy‐decarbamoyloxySTX (dd‐doSTX, 2), the most reduced STX analogue having the tricyclic skeleton, and its analogues, 12β‐deoxy‐doSTX (12β‐d‐doSTX, 3), 12α‐deoxy‐doSTX (12α‐d‐doSTX, 4), and doSTX (5), were synthesized, and these compounds were screened in the toxic microalgae using high‐resolution LCMSMS. dd‐doSTX (2) and 12β‐d‐doSTX (3) were identified in the PSTs‐producing dinoflagellates (Alexandrium catenella, A. pacificum, and/or Gymnodinium catenatum) and in the cyanobacterium Dolichospermum circinale (TA04). doSTX (5), previously isolated from the dinoflagellate G. catenatum, was also identified in D. circinale (TA04). Furthermore, the conversion of 2 to 3, and 4 to 5, by SxtT with VanB, a reported Rieske oxygenase and its redox partner in STX biosynthesis, was confirmed. These results support that 2 is a possible biosynthetic precursor of STX, and that ring and side‐chain hydroxylations proceed after cyclization.

  12. The quite low cross-reactivity of Kawatsu's anti-tetrodotoxin monoclonal antibody to 5,6,11-trideoxytetrodotoxin, 11-nortetrodotoxin-6(S)-ol, and 11-oxotetrodotoxin, the major tetrodotoxin analogues in pufferfish Peer-reviewed

    Keita Yamaki, Kyoka Sato, Yuta Kudo, Yuko Cho, Keiichi Konoki, Tomohiro Takatani, Osamu Arakawa, Kentaro Kawatsu, Mari Yotsu-Yamsahita

    Toxicon　107081-107081　2023/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.toxicon.2023.107081 　

    ISSN： 0041-0101

  13. State-Dependent Inhibition of Voltage-Gated Sodium Channels in Neuroblastoma Neuro-2A Cells by Arachidonic Acid from Halichondria okadai International-journal Peer-reviewed

    Osamu Chiba, Noriko Shimada, Shutaro Yoshio, Yuta Kudo, Yuko Cho, Mari Yotsu-Yamashita, Keiichi Konoki

    Chemical Research in Toxicology　35　(11)　1950-1961　2022/11/21

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.chemrestox.2c00062 　

    ISSN： 0893-228X

    eISSN： 1520-5010

  14. Isolation and Biological Activity of 9-epiTetrodotoxin and Isolation of Tb-242B, Possible Biosynthetic Shunt Products of Tetrodotoxin from Pufferfish International-journal Peer-reviewed

    Yuji Yaegashi, Yuta Kudo, Nozomi Ueyama, Ken-ichi Onodera, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita

    Journal of Natural Products　85　(9)　2199-2206　2022/09/23

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jnatprod.2c00588 　

    ISSN： 0163-3864

    eISSN： 1520-6025

  15. Chemical Ecology of the North American Newt Genera Taricha and Notophthalmus

    Charles T. Hanifin, Yuta Kudo, Mari Yotsu-Yamashita

    Progress in the Chemistry of Organic Natural Products 118　101-130　2022/04/14

    Publisher: Springer International Publishing

    DOI： 10.1007/978-3-030-92030-2_3 　

    ISSN： 2191-7043

    eISSN： 2192-4309

  16. First Identification of 12β-Deoxygonyautoxin 5 (12α-Gonyautoxinol 5) in the Cyanobacterium Dolichospermum circinale (TA04) and 12β-Deoxysaxitoxin (12α-Saxitoxinol) in D. circinale (TA04) and the Dinoflagellate Alexandrium pacificum (Group IV) (120518KureAC) International-journal Invited Peer-reviewed

    Michiru Akamatsu, Ryosuke Hirozumi, Yuko Cho, Yuta Kudo, Keiichi Konoki, Yasukatsu Oshima, Mari Yotsu-Yamashita

    Marine Drugs　20　(3)　166-166　2022/02/25

    Publisher: MDPI AG

    DOI： 10.3390/md20030166 　

    eISSN： 1660-3397

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    Saxitoxin and its analogues, paralytic shellfish toxins (PSTs), are potent and specific voltage-gated sodium channel blockers. These toxins are produced by some species of freshwater cyanobacteria and marine dinoflagellates. We previously identified several biosynthetic intermediates of PSTs, as well as new analogues, from such organisms and proposed the biosynthetic and metabolic pathways of PSTs. In this study, 12β-deoxygonyautoxin 5 (12α-gonyautoxinol 5 = gonyautoxin 5-12(R)-ol) was identified in the freshwater cyanobacterium, Dolichospermum circinale (TA04), and 12β-deoxysaxitoxin (12α-saxitoxinol = saxitoxin-12(R)-ol) was identified in the same cyanobacterium and in the marine dinoflagellate Alexandrium pacificum (Group IV) (120518KureAC) for the first time from natural sources. The authentic standards of these compounds and 12α-deoxygonyautoxin 5 (12β-gonyautoxinol 5 = gonyautoxin 5-12(S)-ol) were prepared by chemical derivatization from the major PSTs, C1/C2, produced in D. circinale (TA04). These standards were used to identify the deoxy analogues by comparing the retention times and MS/MS spectra using high-resolution LC-MS/MS. Biosynthetic or metabolic pathways for these analogues have also been proposed based on their structures. The identification of these compounds supports the α-oriented stereoselective oxidation at C12 in the biosynthetic pathway towards PSTs.

  17. Phylogenetic analysis of the salinipostin γ-butyrolactone gene cluster uncovers new potential for bacterial signalling-molecule diversity Peer-reviewed

    Kaitlin E. Creamer, Yuta Kudo, Bradley S. Moore, Paul R. Jensen

    Microbial Genomics　7　(5)　1-14　2021/05/12

    Publisher: Microbiology Society

    DOI： 10.1099/mgen.0.000568 　

    eISSN： 2057-5858

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    Bacteria communicate by small-molecule chemicals that facilitate intra- and inter-species interactions. These extracellular signalling molecules mediate diverse processes including virulence, bioluminescence, biofilm formation, motility and specialized metabolism. The signalling molecules produced by members of the phylum Actinobacteria generally comprise γ-butyrolactones, γ-butenolides and furans. The best-known actinomycete γ-butyrolactone is A-factor, which triggers specialized metabolism and morphological differentiation in the genus <italic> <named-content content-type="genus"> <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.6817" xlink:type="simple">Streptomyces</ext-link> </named-content> </italic>. Salinipostins A–K are unique γ-butyrolactone molecules with rare phosphotriester moieties that were recently characterized from the marine actinomycete genus <italic> <named-content content-type="genus"> <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9671" xlink:type="simple">Salinispora</ext-link> </named-content> </italic>. The production of these compounds has been linked to the nine-gene biosynthetic gene cluster (BGC) <italic>spt</italic>. Critical to salinipostin assembly is the γ-butyrolactone synthase encoded by <italic>spt9</italic>. Here, we report the surprising distribution of <italic>spt9</italic> homologues across 12 bacterial phyla, the majority of which are not known to produce γ-butyrolactones. Further analyses uncovered a large group of <italic>spt</italic>-like gene clusters outside of the genus <italic> <named-content content-type="genus"> <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9671" xlink:type="simple">Salinispora</ext-link> </named-content> </italic>, suggesting the production of new salinipostin-like diversity. These gene clusters show evidence of horizontal transfer and location-specific recombination among <italic> <named-content content-type="genus"> <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9671" xlink:type="simple">Salinispora</ext-link> </named-content> </italic> strains. The results suggest that γ-butyrolactone production may be more widespread than previously recognized. The identification of new γ-butyrolactone BGCs is the first step towards understanding the regulatory roles of the encoded small molecules in Actinobacteria.

  18. Two new skeletal analogues of saxitoxin found in the scallop, Patinopecten yessoensis, as possible metabolites of paralytic shellfish toxins Peer-reviewed

    Satoshi Numano, Yuta Kudo, Yuko Cho, Keiichi Konoki, Yoshimasa Kaga, Kazuo Nagasawa, Mari Yotsu-Yamashita

    Chemosphere　278　130224-130224　2021/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.chemosphere.2021.130224 　

    ISSN： 0045-6535

  19. Phylogenetic analysis of the salinipostin γ-butyrolactone gene cluster uncovers new potential for bacterial signaling-molecule diversity

    Kaitlin E. Creamer, Yuta Kudo, Bradley S. Moore, Paul R. Jensen

    bioRχiv　2020/10/16

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1101/2020.10.16.342204 　

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    <title>Abstract</title>Bacteria communicate by small-molecule chemicals that facilitate intra- and inter-species interactions. These extracellular signaling molecules mediate diverse processes including virulence, bioluminescence, biofilm formation, motility, and specialized metabolism. The signaling molecules produced by members of the phylum Actinobacteria are generally comprised of γ-butyrolactones, γ-butenolides, and furans. The best known actinomycete γ-butyrolactone is A-factor, which triggers specialized metabolism and morphological differentiation in the genus <italic>Streptomyces</italic>. Salinipostins A-K are unique γ-butyrolactone molecules with rare phosphotriester moieties that were recently characterized from the marine actinomycete genus <italic>Salinispora</italic>. The production of these compounds has been linked to the 9-gene biosynthetic gene cluster <italic>spt</italic>. Critical to salinipostin assembly is the γ-butyrolactone synthase encoded by <italic>spt9</italic>. Here, we report the global distribution of <italic>spt9</italic> among sequenced bacterial genomes, revealing a surprising diversity of gene homologs across 12 bacterial phyla, the majority of which are not known to produce γ-butyrolactones. Further analyses uncovered a large group of <italic>spt</italic>-like gene clusters outside of the genus <italic>Salinispora</italic>, suggesting the production of new salinipostin-like diversity. These gene clusters show evidence of horizontal transfer between many bacterial taxa and location specific homologous recombination exchange among <italic>Salinispora</italic> strains. The results suggest that γ-butyrolactone production may be more widespread than previously recognized. The identification of new γ-butyrolactone biosynthetic gene clusters is the first step towards understanding the regulatory roles of the encoded small molecules in Actinobacteria. <sec><title>Importance</title>Signaling molecules orchestrate a wide variety of bacterial behaviors. Among Actinobacteria, γ-butyrolactones mediate morphological changes and regulate specialized metabolism. Despite their importance, few γ-butyrolactones have been linked to their cognate biosynthetic gene clusters. A new series of γ-butyrolactones called the salinipostins was recently identified from the marine actinomycete genus <italic>Salinispora</italic> and linked to the <italic>spt</italic> biosynthetic gene cluster. Here we report the detection of <italic>spt</italic>-like gene clusters in diverse bacterial families not known for the production of this class of compounds. This finding expands the taxonomic range of bacteria that may employ this class of compounds and provides opportunities to discover new compounds associated with chemical communication. </sec>

  20. Temporal variation of the profile and concentrations of paralytic shellfish toxins and tetrodotoxin in the scallop, Patinopecten yessoensis, cultured in a bay of East Japan International-journal Peer-reviewed

    Satoshi Numano, Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita

    Marine Drugs　17　(12)　653　2019/11

    DOI： 10.3390/md17120653 　

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    Paralytic shellfish toxins (PSTs) are the major neurotoxic contaminants of edible bivalves in Japan. Tetrodotoxin (TTX) was recently detected in bivalve shellfish around the world, drawing widespread attention. In Japan, high levels of TTX were reported in the digestive gland of the scallop, Patinopecten yessoensis, in 1993; however, no new data have emerged since then. In this study, we simultaneously analyzed PSTs and TTX in scallops cultured in a bay of east Japan using hydrophilic interaction chromatography (HILIC)-MS/MS. These scallops were temporally collected from April to December 2017. The highest concentration of PSTs (182 µmol/kg, total congeners) in the hepatopancreas was detected in samples collected on May 23, lined to the cell density of the dinoflagellate, Alexandrium tamarense, in seawater around the scallops, whereas the highest concentration of TTX (421 nmol/kg) was detected in samples collected on August 22. Contrary to the previous report, temporal variation of the PSTs and TTX concentrations did not coincide. The highest concentration of TTX in the entire edible tissues was 7.3 µg/kg (23 nmol/kg) in samples obtained on August 22, which was lower than the European Food Safety Authority (EFSA)-proposed threshold, 44 µg TTX equivalents/kg shellfish meat. In addition, 12β-deoxygonyautoxin 3 was firstly identified in scallops.

  21. Cariogenic Streptococcus mutans produces strain-specific antibiotics that impair commensal colonization

    †Xiaoyu Tang, †Yuta Kudo, Jonathon Baker, Sandra LaBonte, Peter A. Jordan, Shaun M, K. McKinnie, Jian Guo, Tao Huan, Bradley S. Moore, Anna Edlund

    bioRχiv　6　(4)　563-571　2019/09

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acsinfecdis.9b00365 　

    ISSN： 2373-8227

    eISSN： 2373-8227

  22. Isolation and Biological Activity of 8-Epitetrodotoxin and the Structure of a Possible Biosynthetic Shunt Product of Tetrodotoxin, Cep-226A, from the Newt Cynops ensicauda popei Peer-reviewed

    Yuta Kudo, Mari Yotsu-Yamashita

    Journal of Natural Products　82　(6)　1656-1663　2019/05

  23. Total syntheses and determination of absolute configurations of Cep-212 and Cep-210, predicted biosynthetic intermediates of tetrodotoxin isolated from toxic newt Peer-reviewed

    Masaatsu Adachi, Tadachika Miyasaka, Yuta Kudo, Keita Sugimoto, Mari Yotsu-Yamashita, Toshio Nishikawa

    Organic Letters　21　(3)　780-784　2019/01

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.orglett.8b04043 　

    ISSN： 1523-7060

    eISSN： 1523-7052

  24. Structural Elucidation of Trace Components Combining GC/MS, GC/IR, DFT-Calculation and Synthesis-Salinilactones, Unprecedented Bicyclic Lactones from Salinispora Bacteria Peer-reviewed

    Christian Schlawis, Simone Kern, Yuta Kudo, Jörg Grunenberg, Bradley S. Moore, Stefan Schulz

    Angewandte Chemie International Edition　57　(45)　14921-14925　2018/11/05

    Publisher: Wiley

    DOI： 10.1002/anie.201807923 　

    ISSN： 1433-7851

  25. Spiro Bicyclic Guanidino Compounds from Pufferfish: Possible Biosynthetic Intermediates of Tetrodotoxin in Marine Environments Peer-reviewed

    Nozomi Ueyama, Keita Sugimoto, Yuta Kudo, Ken-Ichi Onodera, Yuko Cho, Keiichi Konoki, Toshio Nishikawa, Mari Yotsu-Yamashita

    Chemistry - A European Journal　24　(28)　7250-7258　2018/05/17

    Publisher: Wiley-VCH Verlag

    DOI： 10.1002/chem.201801006 　

    ISSN： 1521-3765 0947-6539

  26. Comparative transcriptomics as a guide to natural product discovery and biosynthetic gene cluster functionality Peer-reviewed

    Gregory C. A. Amos, Takayoshi Awakawa, Robert N. Tuttle, Anne-Catrin Letzel, Min Cheol Kim, Yuta Kudo, William Fenical, Bradley S. Moore, Paul R. Jensen

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　114　(52)　E11121-E11130　2017/12

    DOI： 10.1073/pnas.1714381115 　

    ISSN： 0027-8424

  27. Dietary administration of tetrodotoxin and its putative biosynthetic intermediates to the captive-reared non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster Peer-reviewed

    Yuta Kudo, Chikafumi Chiba, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashita

    TOXICON　137　78-82　2017/10

    DOI： 10.1016/j.toxicon.2017.07.016 　

    ISSN： 0041-0101

  28. フグの新規スピロ環状グアニジノ化合物の単離、構造決定と海産テトロドトキシンの生合成経路の推定

    上山望, 杉本敬太, 工藤雄大, 長由扶子, 此木敬一, 西川俊夫, 山下まり

    第59回天然有機化合物討論会講演要旨集　597-601　2017/08

  29. A new sarasinoside congener, sarasinoside M-2, from a marine sponge collected in the Solomon Islands Peer-reviewed

    Clyde Gorapava Puilingi, Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　81　(2)　222-225　2017/02

    DOI： 10.1080/09168451.2016.1246172 　

    ISSN： 0916-8451

    eISSN： 1347-6947

  30. Screening of novel tetrodotoxin analogues and study of the biosynthesis of tetrodotoxin Peer-reviewed

    Yuta Kudo

    Tohoku University　2016/03

  31. Tetrodotoxin and Its Analogues in the Pufferfish Arothron hispidus and A-nigropunctatus from the Solomon Islands: A Comparison of Their Toxin Profiles with the Same Species from Okinawa, Japan Peer-reviewed

    Clyde Gorapava Puilingi, Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita

    TOXINS　7　(9)　3436-3454　2015/09

    DOI： 10.3390/toxins7093436 　

    ISSN： 2072-6651

  32. Confirmation of the absence of tetrodotoxin and its analogues in the juveniles of the Japanese fire-bellied newt, Cynops pyrrhogaster, captive-reared from eggs in the laboratory using HILIC-LC-MS Peer-reviewed

    Yuta Kudo, Chikafumi Chiba, Keiichi Konoki, Yuko Cho, Mad Yotsu-Yamashita

    TOXICON　101　101-105　2015/07

    DOI： 10.1016/j.toxicon.2015.05.008 　

    ISSN： 0041-0101

  33. C5-C10 Directly Bonded Tetrodotoxin Analogues: Possible Biosynthetic Precursors of Tetrodotoxin From Newts Peer-reviewed

    Yuta Kudo, Yoko Yamashita, Dietrich Mebs, Yuko Cho, Keiichi Konoki, Takeshi Yasumoto, Mari Yotsu-Yamashita

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　53　(52)　14546-14549　2014/12

    DOI： 10.1002/anie.201408913 　

    ISSN： 1433-7851

    eISSN： 1521-3773

  34. Isolation of 6-Deoxytetrodotoxin from the Pufferfish, Takifugu pardalis, and a Comparison of the Effects of the C-6 and C-11 Hydroxy Groups of Tetrodotoxin on Its Activity Peer-reviewed

    Yuta Kudo, Julian Finn, Kohei Fukushima, Satsuki Sakugawa, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita

    JOURNAL OF NATURAL PRODUCTS　77　(4)　1000-1004　2014/04

    DOI： 10.1021/np401097n 　

    ISSN： 0163-3864

    eISSN： 1520-6025

  35. First Identification of 5,11-Dideoxytetrodotoxin in Marine Animals, and Characterization of Major Fragment Ions of Tetrodotoxin and Its Analogs by High Resolution ESI-MS/MS Peer-reviewed

    Mari Yotsu-Yamashita, Yuka Abe, Yuta Kudo, Raphael Ritson-Williams, Valerie J. Paul, Keiichi Konoki, Yuko Cho, Masaatsu Adachi, Takuya Imazu, Toshio Nishikawa, Minoru Isobe

    MARINE DRUGS　11　(8)　2799-2813　2013/08

    DOI： 10.3390/md11082799 　

    ISSN： 1660-3397

  36. Isolation and Structural Determination of the First 8-epi-type Tetrodotoxin Analogs from the Newt, Cynops ensicauda popei, and Comparison of Tetrodotoxin Analogs Profiles of This Newt and the Puffer Fish, Fugu poecilonotus Peer-reviewed

    Yuta Kudo, Takeshi Yasumoto, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashita

    MARINE DRUGS　10　(3)　655-667　2012/03

    DOI： 10.3390/md10030655 　

    ISSN： 1660-3397

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Misc. 18

  1. Origin, accumulation and proposed biosynthetic pathway of tetrodotoxin in terrestrial amphibian, newts and frogs Invited

    Yuta Kudo, Mari Yotsu-Yamashi

    KAIYO MANTHLY　55　(2)　53-59　2023/02

  2. Mystery of the Neurotoxin Tetrodotoxin in the Terrestrial Newt Invited Peer-reviewed

    Yuta Kudo, Mari Yotsu-Yamashita

    KAGAKU TO SEIBUTSU　60　(9)　440-442　2022/09

  3. テトロドトキシンはモノテルペン由来か？

    山下まり, 工藤雄大

    月刊化学　71　(12)　66-67　2016/12

    Publisher: 化学同人

    More details Close

    国際共著論文

  4. Prediction of biosynthetic pathway towards pufferfish toxin, tetrodotoxin Invited

    Mari Yotsu-Yamashita, Yuta Kudo, Nozomi Ueyama, Yuji Yaegashi, Yuko Cho, Keiichi Konoki

    KAIYO MANTHLY　55　(2)　47-52　2023/02

  5. 二枚貝が含有する麻痺性貝毒の代謝物に関する研究

    沼野聡, 渡邊龍一, 内田肇, 小澤眞由, 松嶋良次, 工藤雄大, 工藤雄大, 山下まり, 鈴木敏之

    日本食品衛生学会学術講演会講演要旨集　119th　2023

  6. ホタテガイ(Patinopecten yessoensis)における麻痺性貝毒代謝物の蓄積

    沼野聡, 渡邊龍一, 内田肇, 小澤眞由, 松嶋良次, 工藤雄大, 工藤雄大, 山下まり, 鈴木敏之

    日本水産学会大会講演要旨集(CD-ROM)　2023　2023

  7. Prediction of biosynthetic pathways of the pufferfish toxin, tetrodotoxin International-coauthorship Invited

    MARI YOTSU-YAMASHITA, YUTA KUDO, NOZOMI UEYAMA, CHARLES T. HANIFIN, SATOSHI NUMANO, YUKO CHO, KEIICHI KONOKI

    NIPPON SUISAN GAKKAISHI　88　(5)　423-423　2022/09/15

    Publisher: Japanese Society of Fisheries Science

    DOI： 10.2331/suisan.wa2978-4 　

    ISSN： 0021-5392

    eISSN： 1349-998X

  8. はっけん!イモリ イモリを探求する大学・研究施設 Invited

    林 光武, 関 慎太郎

    はっけん!イモリ　152-152　2022/08

    Publisher: 緑書房

  9. Analysis of Paralytic Shellfish Toxins in the Scallop by LC-MS/MS

    沼野聡, 沼野聡, 加賀克昌, 加賀克昌, 工藤雄大, 工藤雄大, 工藤雄大, 山下まり, 山下まり

    食品衛生研究　72　(3)　2022

    ISSN： 0559-8974

  10. Screening for metabolites of paralytic shellfish toxins in the scallop, Patinopecten yessoensis.

    沼野聡, 沼野聡, 工藤雄大, 工藤雄大, 長由扶子, 此木敬一, 山下まり

    日本農芸化学会大会講演要旨集(Web)　2021　2021

    ISSN： 2186-7976

  11. 有毒イモリより得られた新規テトロドトキシン類縁体および環状グアニジノ化合物

    工藤 雄大, Charles T. Hanifin, 山下 まり

    第62回 天然有機化合物討論会 講演要旨集　337-342　2020/09

  12. Phylogenetic analysis of the salinipostin γ-butyrolactone gene cluster uncovers new bacterial signaling-molecule diversity

    Kaitlin E Creamer, Yuta Kudo, Bradley Moore, Paul R Jensen

    3rd International Conference on Natural Products Discovery and Development in the Genomic Era, Abstract　2020/01

  13. テトロドトキシン推定生合成中間体のテトロドトキシン含有生物への投与

    杉本敬太, 工藤雄大, 宮坂忠親, 安立昌篤, 長由扶子, 此木敬一, 千葉親文, 西川俊夫, 山下まり

    日本農芸化学会大会講演要旨集(Web)　2019　2019

    ISSN： 2186-7976

  14. テトロドトキシンの推定生合成中間体の全合成と絶対立体配置の決定

    宮坂忠親, 安立昌篤, 工藤雄大, 杉本敬太, 山下まり, 西川俊夫

    日本農芸化学会大会講演要旨集(Web)　2019　2019

    ISSN： 2186-7976

  15. 東日本で養殖したホタテガイ中のLC-MS/MSによる麻痺性貝毒分析

    沼野聡, 工藤雄大, 長由扶子, 此木敬一, 山下まり

    日本農芸化学会大会講演要旨集(Web)　2019　2019

    ISSN： 2186-7976

  16. ホタテガイに含有する麻痺性貝毒の代謝物に関する研究

    沼野聡, 沼野聡, 加賀克昌, 工藤雄大, 工藤雄大, 山下まり

    日本食品衛生学会学術講演会講演要旨集　115th　2019

  17. The First Identification of the Novel Tetrodotoxin Analog, 6-Deoxytetrodotoxin, and its Biological Activity

    Y. Kudo, K. Konoki, Y. Cho, M. Yotsu-Yamashita

    PLANTA MEDICA　79　(10)　846-847　2013/07

    ISSN： 0032-0943

    eISSN： 1439-0221

  18. P-33 Isolation and Structural Determination of New Analogs of Tetrodotoxin from Okinawan Newt Cynops ensicauda popei(Poster Presentation)

    Kudo Yuta, Konoki Keiichi, Cho Yuko, Yasumoto Takeshi, Yotsu-Yamashita Mari

    Symposium on the Chemistry of Natural Products, symposium papers　53　(0)　367-372　2011

    Publisher: Symposium on the Chemistry of Natural Products Steering Committee

    DOI： 10.24496/tennenyuki.53.0_367 　

    More details Close

    Tetrodotoxin (TTX) is distributed in wide range of animals, marine puffer fish and terrestrial newts and frogs, for examples. We have isolated various TTX analogs from these animals, and attempted to obtain clues to biosynthetic pathway of TTX from the structures of these analogs. Here we isolated four new analogs of TTX from the Okinawan newt Cynops ensicauda popei, and determined their structures as 8-epi-5,6,11-trideoxyTTX, its 4,9-anhydro derivative, its 1-hydroxy derivative, and its 1-hydroxy-4,4a-anhydro derivative, by spectroscopic method. These analogs are not detected in puffer fish, instead, 5,6,11-trideoxyTTX is detected as a major analog in puffer fish eggs. 1-Hydroxy type analog might be specific to newts, since 1-hydroxy-5,11-dideoxyTTX was previously isolated from the other species of newt, Taricha granulosa, by other group. This is the first identification of 4,4a-anhydro type analog of TTX from the natural source.

Show all ︎Show first 5

Books and Other Publications 2

  1. Progress in the Chemistry of Organic Natural Products, vol 118. Chemical Ecology of the North American Newt Genera Taricha and Notophthalmus

    Charles T. Hanifin, Yuta Kudo, Mari Yotsu-Yamashita

    Springer Nature　2022/04

    ISBN: 9783030920302

  2. テトロドトキシンはモノテルペン由来か？―有毒イモリから発見した新規環状グアニジン化合物

    山下まり, 工藤 雄大

    月刊化学　2016

Presentations 151

  1. Rapid Identification of γ-Butyrolactone Signaling Molecules in Actinomycetes: Discovery of Optical Isomer and Identification of 6-Hydroxy-γ-Butyrolactones

    2025/03/07

  2. Identification of Trace Natural Products Utilizing LCMS: Investigating the Tetrodotoxin Biosynthesis and Actinomycete Signaling Molecules International-presentation Invited

    Yuta Kudo

    SIO Symposium, The Genomic Wave: New Frontiers in Natural Product Discovery　2025/01/10

  3. Rapid identification of γ-butyrolactone signaling molecules in actinomycete through isolation and chemo-enzymatic synthesis International-presentation

    Yuta Kudo, Keiichi Konoki, Mari Yotsu-Yamashita

    5th International Conference on “Natural Product Discovery and Development in the Genomic Era”　2025/01/06

  4. The Origin of Toxins in Amphibians: Focusing on Tetrodotoxin in Newts International-presentation Invited

    Yuta Kudo

    The 2nd NIBB Workshop on Kleptobiology　2024/09/27

  5. Isolation and structural determination of γ-butyrolactone compounds similar to the signaling molecule A-factor, and their concise chemo-enzymatic synthesis and identification in actinomycetes

    Yuta Kudo, Keiichi Konoki, Mari Yotsu-Yamashita

    2024/09/04

  6. Tetrodotoxin analogues and its biosynthetic intermediates in toxic newts

    2024/05/19

  7. Concise chemo-enzymatic synthesis of γ-butyrolactone compounds similar to the signaling molecule A-factor, and their identification in actinomycetes

    JSBBA 2024　2024/03/26

  8. Discovery of new analogues of antimalarial phosphotriester salinipostin using LCMSMS and their inhibitory activity toward monoacyl-glycerol lipase International-presentation

    Yuta Kudo, Keiichi Konoki, Mari Yotsu-Yamashita

    ACS spring 2024　2024/03/20

  9. MS-guided discovery of novel tricyclic guanidino compounds from tetrotodoxin-bearing newts and new analogues of antimalarial phosphotriester salinipostin from actinobacteria International-presentation

    Yuta Kudo, Charles T. Hanifin, Keiichi Konoki, Mari Yotsu-Yamashita

    31st International Symposium on the Chemistry of Natural Products and 11th International Congress on Biodiversity (ISCNP31 & ICOB11)　2023/10/16

  10. MS-guided screening, structural elucidation and evaluation of biological activities of new phophotriesters and γ-butyrolactones

    Yuta KUDO, Keiichi KONOKI, Mari YOTSU-YAMASHITA

    2023/03/14

  11. Screening of novel compounds aimed at elucidating biosynthetic pathway of secondary metabolites Invited

    2022/12/03

  12. Screening of new signaling molecules and phosphotriester compounds from actinomycetes using LC-MS

    2022/09/10

  13. Identification of new compounds from the toxic newts aimed at elucidating the biosynthesis of terrestrial tetrodotoxin Invited

    2022/03/18

  14. Structures of novel analogues and possible biosynthetic intermediates/shunts of tetrodotoxin obtained from toxic newts International-presentation Invited

    Yuta Kudo

    Korea-Japan Joint Workshop on Biofunctional Chemistry　2022/01/12

  15. Identification of new analogues and possible biosynthetic shunt products of terrestrial tetrodotoxin aimed at elucidating its biosynthesis Invited

    Yuta Kudo, Charles Hanifin, Mari Yotsu-Yamashita

    The 2021 International Chemical Congress of Pacific Basin Societies　2021/12/21

  16. Structural Elucidation of Novel Skeletal Tricyclic Guanidino Compounds from the Tetrodotoxin- Bearing Newt, Taricha granulosa

    63th Symposium on the Chemistry of Natural Products　2021/09/17

  17. New tetrodotoxin analogues and cyclic guanidino compounds from toxic newts

    Yuta Kudo, Charles T. Hanifin, Mari Yotsu-Yamashita

    62th Symposium on the Chemistry of Natural Products　2020/09/22

  18. Identification of new analogs and putative biosynthetic intermediates of tetrodotoxin aimed at elucidating its biosynthetic pathway and structure activity relationship International-presentation

    Yuta Kudo, Mari Yotsu-Yamashita

    60 th American Society of Pharmacognosy Annual Meeting 2019　2019/07/16

  19. 海洋放線菌Salinispora由来リン酸トリエステル化合物salinipostinの生合成におけるγ-butyrolactone構造の構築

    工藤 雄大, 淡川孝義, Yi-Ling Du, Peter Jordan, Paul R Jensen, Katherine Ryan, Rojer Linington, Bradley S. Moore

    第60回 天然有機化合物討論会　2018/09

  20. 有毒イモリから得られた新規環状グアニジン化合物群とテトロドトキシン生合成経路の考察

    工藤 雄大, 千葉 親文, 長 由扶子, 此木 敬一, 山下 まり

    第57回 天然有機化合物討論会　2015/09

  21. 新規テトロドトキシン類縁体の構造と生合成経路の推定

    工藤 雄大, 山下 瑶子, 此木 敬一, 長 由扶子, 安元 健, 山下 まり

    第55回 天然有機化合物討論会　2013/09

  22. 珪藻Nitzschia navis-varingica複数株のドウモイ酸生合成遺伝子の発現解析と関連物質の分析

    山下まり, 芦田康洋, 望月政希, 前野優香理, Clyde G. Puilingi, 工藤雄大, 此木敬一, 由扶子, 小瀧裕一

    令和7年度日本水産学会春季大会　2025/03/28

  23. オカダ酸結合タンパク質を用いる新規下痢性貝毒定量法の開発

    此木敬一, 棟本健, 田端滉樹, 内田肇, 渡邊龍一, 長由扶子, 工藤雄大, 山下まり

    令和7年度日本水産学会春季大会　2025/03/28

  24. 日本産アカザラガイChlamys (Azumapecten) farreri akazara中のテトロドトキシンと麻痺性貝毒の組織分布

    松井 蘭丸, 工藤 雄大, 長 由扶子, 此木 敬一, 山下 まり

    日本農芸化学会2025年度大会　2025/03/08

  25. パプアニューギニア産珪藻Nitzschia navis-varingicaのドウモイ酸生合成遺伝子の同定

    芦田 康洋, 望月 政希, Clyde Puilingi, 前野 優香理, 工藤 雄大, 長 由扶子, 此木 敬一, 小瀧 裕一, 山下 まり

    日本農芸化学会2025年度大会　2025/03/05

  26. 麻痺性貝毒サキシトキシンの生合成中間体の合成および三環形成機構の解明

    廣住 燎亮, 袴田 真有, 美野輪 高之, 長 由扶子, 工藤 雄大, 此木 敬一, 大島 泰克, 長澤 和夫, 山下 まり

    日本農芸化学会2025年度大会　2025/03/07

  27. Elucidation of saxitoxin biosynthetic pathway based on the heterologous expression of its biosynthetic enzyme, SxtH

    2025/03/08

  28. サキシトキシンの生合成研究から着想を得たdispacamide類の合成研究

    廣住燎亮, 工藤雄大, 長 由扶子, 此木敬一, 山下まり

    日本農芸化学会東北支部 第159回大会　2024/09/28

  29. 下痢性貝毒オカダ酸の二枚貝中腸腺内のアシル化酵素の探索

    長谷川巧, 長由扶子, 工藤雄大, 山下まり, 此木敬一

    日本農芸化学会東北支部 第159回大会　2024/09/28

  30. サキシトキシン生合成酵素SxtTの異種発現と反応に基づく生合成経路の推定

    青沼栞里, 袴田真有, 廣住燎亮, 工藤雄大, 長 由扶子, 此木敬一, 石塚 颯, 長澤和夫, 山下まり

    日本農芸化学会東北支部 第159回大会　2024/09/28

  31. 放線菌シグナル分子の迅速同定を目指したγ-ブチロラクトン化合物の化学-酵素合成法の構築

    工藤雄大, 此木敬一, 山下まり

    日本生薬学会第70回年会　2024/09/16

  32. 致死性海藻食中毒原因物質ポリカバノシド類の作用機序および新規ポリカバノシド類縁体の構造と毒性

    青木玲翔, 土肥裕花, 岩崎浩太郎, 佐々木 理, 川島悠岐, 島田紀子, 工藤雄大, 長 由扶子, 此木敬一, 佐々木 誠, 山下まり

    第66回天然有機化合物討論会　2024/09/04

  33. Synthetic studies for elucidation of saxitoxin biosynthesis International-presentation

    Ryosuke Hirozumi, Takashi Minowa, Yuko Cho, Yuta Kudo, Keiichi Konoki, Yasukatsu Oshima, Kazuo Nagasawa, Mari Yotsu-Yamashita

    Gordon Research Conference Natural Products and Bioactive Compounds　2024/07/31

  34. Synthetic study for elucidation of saxitoxin biosynthesis International-presentation

    Ryosuke Hirozumi, Takashi Minowa, Yuko Cho, Yuta Kudo, Keiichi Konoki, Yasukatsu Oshima, Kazuo Nagasawa, Mari Yotsu-Yamashita

    FRIS - KKU Joint Symposium 2024 in Sendai, Japan　2024/07/24

  35. Study of saxitoxin biosynthesis using synthetic products International-presentation

    Ryosuke Hirozumi, Takashi Minowa, Yuko Cho, Yuta Kudo, Keiichi Konoki, Kazuo Nagasawa, Yasukatsu Oshima, Mari Yotsu-Yamashita

    GP-Chem Chemistry Summer School 2024　2024/07/20

  36. Chemoenzymatic synthesis of signal molecules, γ-butyrolactones, and their identification in actinomycetes

    2024/06/21

  37. Isolation and structural elucidation of new tetrodotoxin analogues from the pufferfish Chelonodon patoca

    2024/05/18

  38. 藍藻Dolichospermum circinale由来のサキシトキシン生合成酵素SxtTの大腸菌での発現と反応

    山下まり, 青沼栞里, 工藤雄大, 袴田真有, 廣住燎亮, 長 由扶子, 此木敬一, 石塚颯, 長澤和夫

    令和6年度日本水産学会春季大会　2024/03/30

  39. Quantitation of tetrodotoxin and identification of its analogues in two species of bivalves collected in Japan

    2024/03/25

  40. Identification of kainoid synthase of the diatom, Nitzschia navis-varingica, collected from Papua New Guinea International-coauthorship

    2024/03/25

  41. Preparation of novel kainic acid analogues using the heterologously expressed kainoid synthases

    2024/03/25

  42. Synthetic study of a putative biosynthetic intermediate of paralytic shellfish toxin, saxitoxin (2)

    2024/03/26

  43. The possible structure of a new analogue of polycavernoside, the causative toxin of the lethal seaweed poisoning

    2024/03/25

  44. Isolation and structural elucidation of a new tetrodotoxin analogue from the pufferfish Chelonodon patoca

    2024/03/25

  45. 海洋生物毒マイトトキシンの作用機序解明に向けたケミカルプローブ合成

    此木敬一, 中山 健, 大岩弘隆, 駒崎有紀, 工藤雄大, 長 由扶子, 山下まり

    日本農芸化学会東北支部第158回大会　2023/12/02

  46. オカダ酸結合タンパク質OABP2.1を用いる簡易下痢性貝毒定量法の開発

    田端滉樹, 工藤雄大, 長 由扶子, 山下まり, 此木敬一

    日本農芸化学会東北支部第158回大会　2023/12/02

  47. 痺性貝毒サキシトキシンの推定生合成中間体の合成および生合成研究

    廣住燎亮, 長 由扶子, 工藤雄大, 此木敬一, 長澤和夫, 山下まり

    日本農芸化学会東北支部第158回大会　2023/12/02

  48. 二枚貝類における麻痺性貝毒の代謝物 (Мトキシン) 蓄積について

    沼野 聡, 渡邊龍一, 小澤眞由, 内田 肇, 松嶋良次, 加賀新之助, 工藤雄大, 山下まり, 鈴木敏之

    貝毒分科会　2023/11/20

  49. Synthetic study of a putative biosynthetic intermediate of the paralytic shellfish toxin saxitoxin International-presentation

    Ryosuke Hirozumi, Yuko Cho, Yuta Kudo, Keiichi Konoki, Kazuo Nagasawa, Mari Yotsu-Yamashita

    International Symposium in Okinawa, 2023, on Ciguatera and Related Marine Biotoxins.　2023/11/13

  50. Synthesis and identification of decarbamoyloxysaxitoxins in the toxic microalgae for elucidation of saxitoxin biosynthesis International-presentation

    Mari Yotsu-Yamashita, Mayu Hakamada, Chihiro Tokairin, Hayate Ishizuka, Kanna Adachi, Toma Osawa, Ryosuke Hirozumi, Yuko Cho, Yuta Kudo, Keiichi Konoki, Yasukatsu Oshima, Kazuo Nagasawa

    2023/11/13

  51. Chemical studies on biosynthetic and metabolic pathways of marine toxins. International-presentation Invited

    Mari Yotsu-Yamashita, Yuta Kudo, Shigeki Tsuchiya, Satoshi Numano, Yuko Cho, Keiichi Konoki, Yasukatsu Oshima, Kazuo Nagasawa

    20th International Conference on Harmful Algae　2023/11/09

  52. 二枚貝が含有する麻痺性貝毒の代謝物に関する研究.

    沼野 聡, 渡邊龍一, 内田 肇, 小澤眞由, 松嶋良次, 工藤雄大, 山下まり, 鈴木敏之.

    第119 回日本食品衛生学会 学術講演会　2023/10/12

  53. デオキシ-デカルバモイロキシサキシトキシンの有毒藍藻からの同定

    山下まり, 袴田真有, 石塚颯, 安達栞菜, 大澤瞳生, 長由扶子, 工藤雄大, 此木敬一, 大島泰克, 長澤和夫

    令和５年度日本水産学会秋季大会　2023/09/21

  54. ホタテガイ (Patinopecten yessoensis) における麻痺性貝毒代謝物の蓄積.

    沼野 聡, 渡邊龍一, 内田 肇, 小澤眞由, 松嶋良次, 工藤雄大, 山下まり, 鈴木敏之

    令和５年度日本水産学会秋季大会　2023/09/21

  55. デカルバモイロキシサキシトキシン類の合成と有毒生物中からの同定および生合成経路の推定

    袴田真有, 東海林千容, 石塚颯, 安達栞菜, 大澤瞳生, 廣住燎亮, 土屋成輝, 長 由扶子, 工藤雄大, 此木敬一, 大島泰克, 長澤和夫, 山下まり

    第65回天然有機化合物討論会　2023/09/13

  56. Synthetic study of a putative biosynthetic intermediate of the paralytic shellfish toxin saxitoxin International-presentation

    Ryosuke Hirozumi, Yuko Cho, Yuta Kudo, Keiichi Konoki, Kazuo Nagasawa, Mari Yotsu-Yamashita

    International Symposium for the 80th Anniversary of the Tohoku Branch of the Chemical Society of Japan (2023 Joint Meeting of the Tohoku Area Chemistry Societies)　2023/09/10

  57. Structures of new tricyclic guanidino compounds from toxic newt and phosphotriesters from bacteria International-presentation

    Yuta Kudo, Charles T. Hanifin, Keiichi Konoki, Mari Yotsu-Yamashita

    International Symposium for the 80th Anniversary of the Tohoku Branch of the Chemical Society of Japan (2023 Joint Meeting of the Tohoku Area Chemistry Societies)　2023/09/10

  58. Evaluation of inhibitory activities of synthetic saxitoxin derivatives to voltage-gated sodium channels International-presentation

    Takumi Kobayashi, Shutaro Yoshio, Osamu Chiba, Ayato Nureki, Yuka Takayanagi, Hayate Ishizuka, Yuta Kudo, Yuko Cho, Mari Yotsu-Yamashita, Kazuo Nagasawa, Keiichi Konoki

    Tohoku University GP-Chem Chemistry Summer School 2023　2023/08/09

  59. Synthesis and identification of decarbamoyloxysaxitoxins in the toxic microalgae for elucidation of saxitoxin biosynthesis International-presentation

    Mayu Hakamada, Chihiro Tokairin, Hayate Ishizuka, Kanna Adachi, Toma Osawa, Ryosuke Hirozumi, Yuko Cho, Yuta Kudo, Keiichi Konoki, Yasukatsu Oshima, Kazuo Nagasawa, Mari Yotsu-Yamashita

    Tohoku University GP-Chem Chemistry Summer School 2023　2023/08/09

  60. Synthetic study of a putative biosynthetic intermediate of the paralytic shellfish toxin, saxitoxin International-presentation

    Ryosuke Hirozumi, Yuko Cho, Yuta Kudo, Keiichi Konoki, Kazuo Nagasawa, Mari Yotsu-Yamashita

    Tohoku University GP-Chem Chemistry Summer School 2023　2023/08/09

  61. Synthesis and identification of 12,13-deoxydecarbamoylsaxitoxins in toxic microalgae, and prediction of biosynthetic pathway

    2023/04/23

  62. 致死性海藻中毒原因物質ポリカバノシド類の作用機序

    山下まり, 土肥裕花, 岩崎浩太郎, 佐々木理, 川島悠岐, 島田紀子, 工藤雄大, 長 由扶子, 此木敬一, 佐々木誠

    令和５年度 日本水産学会 春季大会　2023/03/24

  63. 電位依存性ナトリウムチャネルに対するサキシトキシン合成誘導体の感受性評価

    小林 巧, 吉尾 柊太郎, 千葉 修, 濡木 絢斗, 髙栁 優夏, 石塚 颯, 工藤 雄大, 長 由扶子, 山下 まり, 長澤 和夫, 此木 敬一

    日本農芸化学会2023年度広島大会　2023/03/15

  64. 多価不飽和脂肪酸による電位依存性ナトリウムチャネルの阻害機構

    吉尾 柊太郎, 千葉 修, 島田 紀子, 工藤 雄大, 長 由扶子, 山下 まり, 此木 敬一

    日本農芸化学会2023年度広島大会　2023/03/15

  65. 麻痺性貝毒サキシトキシンの推定生合成中間体の合成研究

    廣住 燎亮, 長 由扶子, 工藤 雄大, 此木 敬一, 長澤 和夫, 山下 まり

    日本農芸化学会2023年度広島大会　2023/03/15

  66. オキナワフグChelonodon patoca由来の新規テトロドトキシン類縁体の単離、構造決定

    青沼 栞里, 工藤 雄大, 長 由扶子, 此木 敬一, 山下 まり

    日本農芸化学会2023年度広島大会　2023/03/14

  67. パプアニューギニア産珪藻Nitzschia navis-varingicaのドウモイ酸生合成中間体の解析

    芦田 康洋, Puilingi Clyde, 山田 基生, 前野 優香理, 工藤 雄大, 長 由扶子, 此木 敬一, 小瀧 裕一, 山下 まり

    日本農芸化学会2023年度広島大会　2023/03/14

  68. サキシトキシンの推定生合成中間体の合成と有毒藍藻および渦鞭毛藻における同定

    袴田 真有, 石塚 颯, 安達 栞菜, 大澤 瞳生, 東海林 千容, 長 由扶子, 工藤 雄大, 此木 敬一, 大島 泰克, 長澤 和夫, 山下 まり

    日本農芸化学会2023年度広島大会　2023/03/14

  69. Preparation of domoic acid and kainic acid analogues using the cyclases, and evaluation of their toxicities International-presentation

    Motomi Yamada, Yukari Maeno, Yuichi Kotaki, Ryuta Terada, Masafumi Hidaka, Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita

    Tohoku University – OIST 3rd Joint Workshop on Biodiversity　2022/10/24

  70. Prediction of biosynthetic pathways of major marine toxins based on their structural diversity International-presentation Invited

    Mari Yotsu-Yamashita, Yuta Kudo, Shigeki Tsuchiya, Satoshi Numano, Yuko Cho, Keiichi Konoki, Kazuo Nagasawa

    Tohoku University – OIST 3rd Joint Workshop on Biodiversity　2022/10/24

  71. Screening for Nav inhibitors from the marine sponge Halichondria okadai and characterization of fatty acid modulation of Nav International-presentation

    Shutaro Yoshio, Osamu Chiba, Noriko Shimada, Yuta Kudo, Yuko Cho, Mari Yotsu, Yamashita, Keiichi Konoki

    Tohoku University – OIST 3rd Joint Workshop on Biodiversity　2022/10/24

  72. 電位依存性ナトリウムチャネルを阻害する核酸アプタマーの探索

    小林 巧, 鳥海優人, 工藤雄大, 長 由扶子, 山下まり, 此木敬一

    令和4年度 日本農芸化学会 北海道・東北支部 合同支部会　2022/09/21

  73. 麻痺性貝毒サキシトキシンの二環性推定生合成中間体の合成研究

    廣住燎亮, 長 由扶子, 工藤雄大, 此木敬一, 長澤和夫, 山下まり

    令和4年度 日本農芸化学会 北海道・東北支部 合同支部会　2022/09/21

  74. Screening of new signaling molecules in actinobacteria

    2022/09/21

  75. 環化酵素を利用したドウモイ酸とカイニン酸類縁体の調製と毒性評価

    山田基生, 前野優香理, 小瀧裕一, 寺田竜太, 日高將文, 工藤雄大, 長由扶子, 此木敬一, 山下まり

    第64回天然有機化合物討論会　2022/09/08

  76. Identification of new tetrodotoxin-related compounds from the toxic newts aimed at elucidating its biosynthesis International-coauthorship

    2022/09/05

  77. 2種の12β-デオキシサキシトキシン類縁体の 有毒藍藻および渦鞭毛藻からの同定

    赤松みちる, 廣住燎亮, 長 由扶子, 工藤雄大, 此木敬一, 大島泰克, 山下まり

    第33回万有仙台シンポジウム　2022/05/21

  78. フグ毒テトロドトキシンの生合成経路の推定 International-coauthorship Invited

    山下まり, 工藤雄大, 上山 望・Charles T. Hanifin, 沼野 聡, 長 由扶子, 此木敬一

    令和4年度日本水産学会春季大会ミニシンポジウム フグ毒と麻痺性貝毒の産生と動態に 関する研究の現状と展望　2022/03/29

  79. 12β-deoxysaxitoxinの藍藻Dolichospermum circinale (TA04) および渦鞭毛藻 Alexandrium pacificum (Group IV)からの同定

    山下まり, 赤松みちる, 長 由扶子, 此木敬一, 工藤雄大, 大島泰克

    令和4年度日本水産学会春季大会　2022/03/24

  80. クロイソカイメンより単離したアラキドン酸と各種脂肪酸による電位依存性ナトリウムチャネルの阻害作用

    千葉 修, 島田 紀子, 工藤 雄大, 長 由扶子, 山下 まり, 此木 敬一

    日本農芸化学会2022年度大会　2022/03/17

  81. 異種発現環化酵素を用いた新規カイニン酸類縁体の調製

    山田 基生, 前野 優香理, 小瀧 裕一, 寺田 竜太, 工藤 雄大, 長 由扶子, 此木 敬一, 山下 まり

    日本農芸化学会2022年度大会　2022/03/16

  82. サキシトキシンの推定生合成中間体の合成と有毒淡水産藍藻における同定

    袴田 真有, 東海林 千容, 長 由扶子, 安達 栞菜, 石塚 颯, 工藤 雄大, 此木 敬一, 大島 泰克, 長澤 和夫, 山下 まり

    日本農芸化学会2022年度大会　2022/03/16

  83. 抗テトロドトキシンモノクローナル抗体の 主要テトロドトキシン類縁体に対する交差反応性評価

    八巻慶汰, 佐藤恭佳, 工藤雄大, 川津健太郎, 荒川修, 高谷智裕, 長由扶子, 此木敬一, 山下まり

    日本農芸化学会2022年度大会　2022/03/17

  84. Two novel skeletal tricyclic guanidino compounds from the tetrodotoxin-bearing newt International-coauthorship

    2022/03/16

  85. Possible mode of action of polycavernosides, and Nav blocking activities of highly purified tetrodotoxin analogues Invited

    Mari Yotsu-Yamashita, Yuka Doi, Kotaro, Iwasaki, Satori Sasaki, Yuki Kawashima, Noriko Shimada, Osamu Chiba, Kyoka Sato, Yuta Kudo, Yuko Cho, Keiichi Konoki, Makoto Sasaki

    The 2021 International Chemical Congress of Pacific Basin Societies　2021/12/20

  86. Prediction of biosynthetic pathway towards tetrodotoxin based on the structures of its natural analogues Invited

    Mari Yotsu-Yamashita, Yuta Kudo, Yuji Yaegashi, Satoshi Numano, Nozomi Ueyama | Charles Hanifin, Yuko Cho, Keiichi Konoki

    The 2021 International Chemical Congress of Pacific Basin Societies　2021/12/21

  87. Hemiaminal type saxitoxin analogues found in the scallop, Patinopecten yessoensis,

    Mari Yotsu-Yamashita, Satoshi Numano, Yuta Kudo, Yuko Cho, Keiichi Konoki, Yoshimasa Kaga, Kazuo Nagasawa

    2021/10/14

  88. 二次元NMR long range-HSQMBCを用いた構造解析の有用性の検証

    工藤雄大, 海原大輔, Charles T. Hanifin, 長 由扶子, 此木敬一, 山下まり

    第156回 農芸化学会東北支部大会　2021/10/09

  89. Identification of New Analogues and Putative Biosynthetic Intermediates/Shunts of Tetrodotoxin from Toxic Newts

    Yuta Kudo, Charles T. Hanifin, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamshita

    ACS Publications Symposium: The Power of Chemical Transformations　2021/05/20

  90. Structure elucidation of novel skeletal tricyclic guanidino compounds

    2021/05/15

  91. テトロドトキシン類縁体のマウス毒性の再確認およびテトロドトキシンのアルカリ初期分解物の単離と構造

    山下まり, 八重樫優士, 佐藤恭佳, 杉本亜津子, 長 由扶子, 此木敬一, 工藤雄大

    令和3年度日本水産学会春季大会　2021/03/27

  92. New cyclic guanidino compounds from the American tetrodotoxin-bearing newts

    2021/03/19

  93. 「天狗の麦飯」から分離されたクテドノバクテリア（Dictyobacter alpinus Uno16）からの抗菌化合物の探索

    齋藤 彩菜, 白石 太郎, 葛山 智久, 新谷 尚弘, 工藤 雄大, 山下 まり, 阿部 敬悦, 横田 明, 矢部 修平

    日本農芸化学会2021年度大会　2021/03/19

  94. ホタテガイ (Patinopecten yessoensis) 中における麻痺性貝毒代謝物の探索

    沼野 聡, 工藤 雄大, 長 由扶子, 此木 敬一, 山下 まり

    日本農芸化学会2021年度大会　2021/03/19

  95. テトロドトキシン-タンパク質複合体作製のモデル反応と主生成物の構造決定

    善 瑞穂, 工藤 雄大, 長 由扶子, 此木 敬一, 山下 まり

    日本農芸化学会2021年度大会　2021/03/19

  96. フグ由来の新規テトロドトキシン関連化合物

    八重樫 優士, 上山 望, 工藤 雄大, 長 由扶子, 此木 敬一, 山下 まり

    日本農芸化学会2021年度大会　2021/03/19

  97. LC-MS/MSを用いた麻痺性貝毒研究

    沼野 聡, 加賀 克昌, 工藤 雄大, 山下 まり

    貝毒分科会　2020/11/24

  98. ヒト虫歯菌が生産するテトラミン酸系抗生物質

    工藤 雄大, Xiaoyu Tang, Jonathon, L. Baker, Anna Edlund, Bradley S. Moore

    日本農芸化学会東北支部 第155 大会　2020/11/07

  99. ホタテガイ中腸腺由来オカダ酸アシル基転移酵素の可溶化条件の検討

    駒崎有紀, 寺内将斗, 工藤雄大, 長 由扶子, 山下まり, 此木敬一

    第155回 農芸化学会東北支部大会　2020/11/07

  100. Structures of new tetrodotoxin analogues and possible biosynthetic products of tetrodotoxin obtained from toxic newts

    Yuta Kudo, Charles T. Hanifi, Mari Yotsu-Yamashita

    2020/10/17

  101. 高純度テトロドトキシン類縁体の定量とNav 阻害活性

    山下まり, 佐藤恭佳, 千葉 修, 長 由扶子, 此木敬一, 工藤雄大

    令和2年度日本水産学会春季大会　2020/03/29

  102. 有毒イモリより得られたN-hydroxy型テトロドトキシン類縁体の構造解析

    工藤 雄大, ハニフィン チャールス, 小瀧 裕一, 山下 まり

    日本農芸化学会2020年度大会　2020/03/05

  103. Isolation and structure elucidation of novel tetrodotoxin related compounds from pufferfish

    Yuji YAEGASHI, Yuta KUDO, Yuko CHO, Keiichi KONOKI, Mari YAMASHITA

    2020/03/05

  104. Phylogenetic analysis of the salinipostin γ-butyrolactone gene cluster uncovers new bacterial signaling-molecule diversity

    Kaitlin E. Creamer, Yuta Kudo, Bradley Moore, Paul R. Jensen

    3rd International Conference on Natural Product Discovery and Development in the Genomic Era　2020/01/12

  105. 岩手県産ホタテガイの中腸腺に含有する麻痺性貝毒の分析

    沼野 聡, 加賀克昌, 工藤雄大, 山下まり

    第56回 全国衛生化学技術協議会年会　2019/12/05

  106. LC-MS/MSを用いた抗マラリア活性天然物サリニポスチンの新規類縁体の探索

    工藤 雄大, 山下 まり

    日本農芸化学会東北支部 第154 大会　2019/11/09

  107. 次世代研究者からの研究紹介～他の分野の研究ってどんな感じ？～ Invited

    工藤 雄大

    連携型博士研究人材総合育成システム 次世代研究者シンポジウム2019　2019/10/31

  108. ホタテガイに含有する麻痺性貝毒の代謝物に関する研究

    沼野 聡, 加賀克昌, 工藤雄大, 山下まり

    第115回日本食品衛生学会学術講演会　2019/10/04

  109. テトロドトキシンの推定生合成中間体の全合成と絶対立体配置の決定

    宮坂 忠親, 安立 昌篤, 工藤 雄大, 杉本 敬太, 山下 まり, 西川 俊夫

    第61回天然有機化合物討論会　2019/09/11

  110. 陸上におけるテトロドトキシンの起源と生合成経路の研究 Invited

    工藤 雄大

    第14回化学生態学研究会　2019/06/29

  111. 高純度テトロドトキシン類縁体の調製

    山下まり, 佐藤恭佳, 工藤雄大, 長 由扶子, 此木敬一

    平成31年度 日本水産学会大会春季大会　2019/03/28

  112. Administration of possible biosynthetic intermediates of tetrodotoxin to tetrodotoxin possessing animals

    Keita Sugimoto, Yuta Kudo, Tadachika Miyasaka, Masaatsu Adachi, Yuko Cho, Keiichi Konoki, Chikafumi Chiba, Toshio Nishikawa, Mari Yotsu-Yamashita

    2019/03/26

  113. Structures and bioactivities of new tetrodotoxin analogue and biosynthetic shunt compound from the newt

    Yuta Kudo, Mari Yotsu-Yamashita

    2019/03/26

  114. Analysis of paralytic shellfish toxins in the scallop cultured in a coast of eastern Japan by LC-MS/MS

    Satoshi Numano, Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita

    2019/03/26

  115. Total Syntheses and Determination of Absolute Configurations of Predicted Biosynthetic Intermediates of Tetrodotoxin

    Tadachika Miyasaka, Masaatsu Adachi, Yuta Kudo, Keita Sugimoto, Mari Yotsu-Yamashita, Toshio Nishikawa

    2019/03/24

  116. Identification of seven possible biosynthetic intermediates of tetrodotoxin in marine environments isolated from pufferfish Invited

    Mari Yotsu-Yamashita, Nozomi Ueyama, Keita Sugimoto, Yuji Yaegashi, Yuta Kudo, Ken-ichi Onodera, Yuko Cho, Keiichi Konoki, Toshio Nishikawa

    CHA 2018 18th International conference on Harmful Algae　2018/10/25

  117. 抗マラリア活性化合物サリニポスチンと新規γ-ブチロラクトンの生合成研究

    工藤雄大, Bradley S. Moore

    日本農芸化学会東北・北海道合同支部 （東北支部 第153回大会）　2018/09

  118. Investigations of the biosynthesis and signaling function of salinipostin, an unusual phosphotriester gamma-butyrolactone compound from Salinispora International-presentation

    Yuta Kudo, Takayoshi Awakawa, Yi-Ling Du, Peter Jordan, Katherine Ryan, Rojer Linington, Bradley S. Moore

    2nd International Conference on Natural Product Discovery and Development in the Genomic Era, Society for Industrial Microbiology & Biotechnology　2018/01

  119. Investigating salinipostin biosynthesis and function, a novel phosphotriester bacterial molecule

    Yuta Kudo

    Third Annual Chemistry & Biochemistry Postdoctoral Research Symposium　2017/10/06

  120. フグの新規スピロ環状グアニジノ化合物の単離、構造決定と海産テトロドトキシンの生合成経路の推定

    上山望, 杉本敬太, 工藤雄大, 此木敬一, 西川俊夫, 山下まり

    第59回天然有機化合物討論会　2017/09

  121. Prediction of biosynthetic pathways of tetrodotoxin and saxitoxin on the basis of the structures of their intermediates International-presentation

    Mari Yotsu-Yamashita, Yuta Kudo, Shigeki Tsuchiya, Yuko Cho, Keiichi Konoki

    US-Japan Symposium in Marine Bioorganic Chemistry: 21st Century Innovations in Natural Products　2016/11

  122. フグから得られた新規テトロドトキシン関連化合物の単離と構造

    上山望, 工藤雄大, 長由扶子, 此木敬一, 山下まり

    日本農芸化学会2016年度大会　2016/03

  123. Screening of bioactive compounds in pufferfish and marine invertebrates from Solomon Islands

    Clyde Gorapava Puilingi, Yuta Kudo, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashita

    2016/03

  124. テトロドトキシンの生合成経路解明を目指した有毒イモリからの推定生合成中間体の探索

    工藤 雄大, 長 由扶子, 此木 敬一, 山下 まり

    日本農芸化学会2016年度大会　2016/03

  125. Our recent studies of tetrodotoxin, saxitoxin and polycavernosides International-presentation

    Mari Yotsu-Yamashita, Yuta Kudo, Shigeki Tsuchiya, Kosuke Maeta, Yuko Cho, Keiichi Konoki

    The 2015 International Chemical Congress of Pacific Basin Societies　2015/12

  126. Screening and structure elucidation of the possible biosynthetic intermediates of tetrodotoxin from the newts International-presentation

    Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita

    The 2015 International Chemical Congress of Pacific Basin Societies　2015/12

  127. イモリにおけるテトロドトキシンの起源および蓄積能の調査と考察

    工藤 雄大, 千葉親文, 長 由扶子, 此木 敬一, 山下 まり

    日本農芸化学会東北支部 第150回大会　2015/10

  128. Tetrodotoxin study in pufferfish and screening of bioactive compounds in marine invertebrates from Solomon Islands International-presentation

    Clyde Gorapava Puilingi, Yuta Kudo, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashit

    Tohoku University’s Chemistry Summer School 2015　2015/08

  129. Identification of analogues and biosynthetic intermediates of tetrodotoxin aimed at elucidating its biosynthesis International-presentation Invited

    Yuta Kudo

    Tohoku University’s Chemistry Summer School 2015　2015/08

  130. 生合成経路解明を目指した新規テトロドトキシン類縁体、生合成中間体の探索

    工藤 雄大

    新学術領域研究「天然物ケミカルバイオロジー～分子標的と活性制御～」第7回若手研究者ワークショップ　2015/06

  131. 質量分析器を用いた新規テトロドトキシン類縁体の探索とその構造解析

    工藤 雄大, 山下 瑤子, 長 由扶子, 此木 敬一, 安元 健, 山下 まり

    第29回海洋生物活性談話会　2015/05

  132. Investigation of tetrodotoxin in pufferfish and screening of bioactive compounds in marine invertebrates from Solomon Islands

    Clyde Gorapava Puilingi, Yuta Kudo, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashita

    2015/03

  133. 有毒イモリからのテトロドトキシンの推定生合成中間体の探索

    工藤 雄大, 此木 敬一, 長 由扶子, 山下 まり

    日本農芸化学会2015年度大会　2015/03

  134. Approach to elucidation of biosynthetic pathways towards tetrodotoxin and saxitoxin International-presentation

    Mari Yotsu-Yamashita, Yuta Kudo, Shigeki Tsuchiya, Yuko Cho, Keiichi Konoki

    Tohoku University’s Chemistry Summer school　2014/08

  135. Screening of tetrodotoxin in pufferfish from Solomon Islands International-presentation

    Clyde Gorapava Puilingi, Yuta Kudo, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashita

    Tohoku University’s Chemistry Summer school　2014/08

  136. Screening of New Tetrodotoxin Analogues Using HILIC-LC-MS/MS and Their Structure Determination International-presentation

    Yuta Kudo, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashita

    Tohoku University’s Chemistry Summer school　2014/08

  137. Structure elucidation of 10-hemiketal-type tetrodotoxin analogues, indicative of the biosynthetic pathway International-presentation

    Yuta Kudo, Yoko Yamashita, Keiichi Konoki, Yuko Cho, Takeshi Yasumoto, Mari Yotsu-Yamashita

    Gordon Research Conference: Natural Products　2014/07

  138. オキナワシリケンイモリの新規テトロドトキシン類縁体の構造、および各種有毒イモリにおける分布

    工藤 雄大, 此木 敬一, 長 由扶子, 山下 まり

    第9回化学生態学研究会　2014/06

  139. フグ毒テトロドトキシン新規類縁体の構造と生理活性、および含有生物の毒組成解析

    工藤 雄大, 此木 敬一, 長 由扶子, 山下 まり

    第25回記念 万有仙台シンポジウム　2014/06

  140. メタゲノムからの新規Baeyer-Villiger monooxygenaseのスクリーニング

    山下 瑤子, 山下 まり, 工藤 雄大, 長 由扶子, 此木 敬一

    日本農芸化学会2014年度大会　2014/03

  141. 10-hemiketal型テトロドトキシン類縁体の構造決定と生合成経路の考察

    工藤 雄大, 山下 瑤子, 此木 敬一, 長 由扶子, 安元 健, 山下 まり

    日本農芸化学会2014年度東京大会　2014/03

  142. Identification of 5,11-dideoxytetrodotoxin in marine animals, and characterization of ESI-MS fragment ions of tetrodotoxin and its analogs International-presentation

    Mari Yotsu-Yamashita, Yuka Abe, Yuta Kudo, Keiichi Konoki, Yuko Cho, Toshio Nishikawa, Minoru Isobe

    2013/09

  143. The structure of the novel tetrodotoxin analog, 6-deoxytetrodotoxin, and structure activity relationship study on C-6 and C-11 hydroxyl groups of tetrodotoxin International-presentation

    Yuta Kudo, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashita

    2013/09

  144. The first identification of the novel tetrodotoxin analog, 6-deoxytetrodotoxin, and its biological activity International-presentation

    Yuta Kudo, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashita

    The American Society of Pharmacognosy’s 2013 annual meeting　2013/09

  145. 新規テトロドトキシン類縁体6-デオキシテトロドトキシンの単離・構造決定

    工藤 雄大, 此木 敬一, 長 由扶子, 山下 まり

    日本農芸化学会2013年度仙台大会　2013/03

  146. HILIC-ESI-Q-TOF-MSを用いたテトロドトキシン類縁体の微量分析法の検討

    工藤 雄大, 此木 敬一, 長 由扶子, 山下 まり

    日本農芸化学会東北支部 第147回大会　2012/10

  147. Isolation and Structural Determination of the First 8-epi-type Tetrodotoxin Analogs from the Newt, Cynops ensicauda popei, and Comparison of Tetrodotoxin Analogs Profiles of This Newt and the Puffer Fish, Fugu poecilonotus International-presentation

    Yuta Kudo, Takeshi Yasumoto, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashita

    2012/08

  148. 沖縄産シリケンイモリ(Cynops ensicauda popei)の新規テトロドトキシン類縁体の単離・構造決定、及びイモリとフグの毒組成比較

    工藤 雄大, 此木 敬一, 長 由扶子, 安元 健, 山下 まり

    第7回化学生態学研究　2012/06

  149. 海洋生物とイモリからの新規テトロドトキシン類縁体の同定

    工藤雄大, 阿部由佳, 山下まり, 西川俊夫, 磯部 稔, 安元 健

    平成24年度日本水産学会春季大会　2012/03

  150. 沖縄産シリケンイモリ (Cynops ensicauda popei)の新規テトロドトキシン類縁体の単離・構造決定、及びイモリとフグの毒組成比較

    工藤 雄大, 此木 敬一, 長 由扶子, 安元 健, 山下 まり

    日本農芸化学会2012年度大会　2012/03

  151. 沖縄産シリケンイモリ (Cynops ensicauda popei)の新規テトロドトキシン類縁体の単離と構造決定

    工藤 雄大, 此木 敬一, 長 由扶子, 安元 健, 山下 まり

    第53回 天然有機化合物討論会　2011/09

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Research Projects 10

  1. Identification of signaling molecules in various actinomycetes and application for secondary metabolism activation Competitive

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2024/04 - 2027/03

  2. 二次代謝制御を目指した放線菌シグナル分子の化学-酵素合成と迅速同定法の開発

    Offer Organization: 公益財団法人野田産業科学研究所

    System: 2025年度「野田産研研究助成」奨励研究助成

    2025/04 - 2026/03

  3. 陸上におけるテトロドトキシンの生合成経路の解明

    Offer Organization: 上原記念生命科学財団

    System: 2024年度研究奨励金

    2025/04 - 2026/03

  4. 化合物生産を制御する放線菌シグナル分子の迅速同定法の開発と有用天然化合物の生産への応用 Competitive

    工藤雄大

    Offer Organization: 公益財団法人矢崎科学技術振興記念財団

    System: 奨励研究助成

    2024/03 - 2025/02

  5. Investigation of the biosynthesis of terrestrial tetrodotoxin via new compound screening and metagenomic analysis

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    Category: Grant-in-Aid for Early-Career Scientists

    Institution: Tohoku University

    2022/04 - 2024/03

  6. 陸上における神経毒テトロドトキシンの生合成経路の解明

    工藤 雄大

    Offer Organization: 内藤記念科学振興財団

    System: 2020年度 内藤記念科学奨励金・研究助成

    Institution: 東北大学

    2021/01 - 2023/06

  7. Elucidation of the biosynthetic pathway of terrestrial tetrodotoxin via metagenomic analysis

    Yuta Kudo

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    Category: Grant-in-Aid for Early-Career Scientists

    Institution: Tohoku University

    2020/04 - 2022/03

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    The biosynthesis of tetrodotoxin (TTX), a potent neurotoxin distributed in pufferfish and newt, has not yet been elucidated. With the aim of elucidating the biosynthetic pathway of TTX in terrestrial organisms, we conducted metagenomic analysis of environmental DNA obtained from the toxic newt habitats, and screening for new TTX-related compounds from toxic newts. New TTX analogues was identified from the toxic newt extract. The structure of the previously reported TTX analogue was revised in this study. We discovered novel cyclic guanidino compounds including two novel skeletal tricyclic compounds. The chemical structures of the obtained compounds provided insight into the biosynthetic/shunt pathways of TTX in terrestrial organisms. The fosmid metagenomic library was prepared from the environmental samples of the toxic newt habitat. PCR screening of possible biosynthetic genes of TTX and sequencing of the library by Next Generation Sequencer were performed.

  8. 化学分析とメタゲノム解析に基づく、陸上におけるテトロドトキシンの起源と生合成 Competitive

    工藤 雄大

    Offer Organization: 日本学術振興会

    System: 研究活動スタート支援

    2018/04 - 2020/03

  9. 海洋放線菌由来シグナル分子の生合成と生体内機能の解析 Competitive

    工藤 雄大

    Offer Organization: 日本学術振興会

    System: 海外特別研究員

    2016/04 - 2018/03

  10. テトロドトキシンの生合成経路の解明－ＬＣ／ＭＳを用いた微量生合成中間体の探索－ Competitive

    工藤 雄大

    Offer Organization: 日本学術振興会

    System: 特別研究員

    2013/04 - 2016/03

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Teaching Experience 10

  1. Advanced Lecture on Food Science　Tohoku University

  2. 生命化学A　東北大学

  3. Advanced Bioorganic Chemistry　Tohoku University

  4. Food and Chemistry　Tohoku University

  5. Chemistry of Biomolecules　Tohoku University

  6. 学問論演習　東北大学全学教育科目

  7. 生命機能物質分析特論　東北大学

  8. Biology B　Tohoku University

  9. Experiments in Agricultural Chemistry II　Tohoku University

  10. 現代における農と農学　東北大学

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Other 2

  1. C&EN Bacteria behind tooth decay make antibiotics that take out their competitors

    More details Close

    C&EN News, Bacteria behind tooth decay make antibiotics that take out their competitors by Jyoti Madhusoodanan, special to C&EN January 30, 2020 (ACS Infect. Dis. 2020, DOI: 10.1021/acsinfecdis.9b00365).

  2. Nature Chemical Biology: Research Highlights

    More details Close

    Natural products: A painful discovery. Nature Chemical Biology 10, 325 (2014), Catherine Goodman, doi:10.1038/nchembio.1516, Published online 17 April 2014.