Details of the Researcher

PHOTO

Takashi Nakamura
Section
Graduate School of Dentistry
Job title
Associate Professor
Degree

  • 歯学 (Osaka University)

e-Rad No.
90585324

Research History 4

  • 2007/10 - 2009/03
    Thomas Jefferson University

  • 2004/09 - 2007/09
    National Institute of Dental and Craniofacial Research　職員研究員

  • 1999/11 - 2004/08
    National Institute of Dental and Craniofacial Resarch　Visiting Fellow

  • 1998/06 - 1999/10
    関西ろうさい病院

Education 2

  • Osaka University　Graduate School, Division of Dental Research　口腔外科学

    - 1998/03/25

  • Hiroshima University　Faculty of Dentistry　歯学科

    - 1994/03/25

Professional Memberships 6

  • The Japanese Pharmacological Society

    2015/10 - Present

  • THE JAPANESE SOCIETY OF PEDIATRIC DENTISTRY

  • THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE

  • THE JAPANESE BIOCHEMICAL SOCIETY

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

  • JAPANESE ASSOCIATION FOR ORAL BIOLOGY

︎Show all ︎Show first 5

Research Interests 18

  • Molecular biology

  • Endocrinology

  • Developmental biology

  • Cell biology

  • gender difference

  • 栄養学

  • enamel

  • cancer

  • Pharmacology

  • Morphogenesis

  • Organogenesis

  • Salivary gland

  • epiprofin

  • cell cycle

  • vitaminD

  • parathyroid

  • molecular biology

  • tooth development

Research Areas 3

  • Life sciences / Oral medicine /

  • Life sciences / Regenerative dentistry and dental engineering /

  • Life sciences / Oral medicine /

Awards 6

  1. 優秀演題賞

    2018/02　第39回東北骨代謝・骨粗鬆症研究会　新規転写因子エピプロフィンによる副甲状ホルモン発現制御機構

  2. 小児歯科学会優秀発表賞

    2010/05/20　日本小児歯科学会

  3. NIDCR Incentive Award

    2007/10　National Institute of Dental and Craniofacial Research

  4. NIDCR Individual Cash Award

    2006/11　National Institute of Dental and Craniofacial Research

  5. NIH Fellows Award for Research Excellence

    2005/01　National Institutes of Health

  6. Japan Society for the Promotion of Sciences Fellowship Award

    2001/01　NIH

Show all ︎Show 5

Papers 115

  1. Molecular mechanism for transcriptional regulation of the parathyroid hormone gene by epiprofin. International-journal

    Takashi Nakamura, Hannah M Nakamura, Yasumasa Iwasaki, Motomi Enomoto-Iwamoto, Noriaki Nakashima, Satoshi Fukumoto, Maurizio Pacifici, Masahiro Iwamoto, Minoru Wakamori

    The FEBS journal　2025/03/31

    DOI： 10.1111/febs.70085 　

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    Epiprofin (Epfn), an Sp/KLF family transcription factor that regulates cell proliferation and determines cell fates, is essential for normal skin, hair follicle, and tooth development. We found that Epfn was expressed in parathyroid glands, and Epfn-knockout mice displayed elevated serum parathyroid hormone (PTH) concentrations, decreased bone volume, and intracranial ectopic calcification. To investigate the role of Epfn in the regulation of PTH expression, parathyroid gland explant and parathyroid cell line culture methods were used. Epfn expression was found to be upregulated in response to an increase in extracellular calcium concentration, whereas PTH expression was downregulated, thus demonstrating an inverse correlation. Forced expression of Epfn inhibited PTH gene expression and PTH promoter reporter activity in parathyroid cells. In addition, with a high extracellular calcium concentration, Epfn silencing in cultured parathyroid glands failed to block PTH gene expression. ChIP-qPCR analysis also revealed Epfn binding in the proximal region of the PTH promoter, which was accelerated in the presence of a high concentration of calcium ions. The results from our in vitro and ex vivo analyses suggest that Epfn is a newly identified negative regulator of PTH transcription by regulating the proximal PTH promoter. Furthermore, the expression of Epfn was significantly reduced in parathyroid adenomas of primary hyperparathyroidism patients. The identification of Epfn as a potential therapeutic target for the control of PTH production in hyperparathyroidism patients opens new avenues for targeted treatment approaches.

  2. Disrupted odontoblast differentiation and dentin dysplasia in Epiprofin-deficient mice. International-journal Peer-reviewed

    Lucia Jiménez-Rojo, Susana de Vega, Gaskon Ibarretxe, Takashi Nakamura, Fernando J Unda

    The International journal of developmental biology　68　(1)　19-24　2024

    DOI： 10.1387/ijdb.240029lj 　

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    Tooth formation is a process tightly regulated by reciprocal interactions between epithelial and mesenchymal tissues. These epithelial-mesenchyme interactions regulate the expression of target genes via transcription factors. Among the regulatory elements governing this process, Epiprofin/Sp6 is a zinc finger transcription factor which is expressed in the embryonic dental epithelium and in differentiating pre-odontoblasts. Epiprofin knockout (Epfn-/-) mice present severe dental abnormalities, such as supernumerary teeth and enamel hypoplasia. Here, we describe dentin defects in molars and incisors of Epfn-/- mice. We observed that in the absence of Epfn, markers of early odontoblast differentiation, such as alkaline phosphatase activity, Dsp/Dpp expression, and Collagen Type I deposition, are downregulated. In addition, the expression of tight and gap junction proteins was severely impaired in the predontoblastic cell layer of developing Epfn-/- molars. Altogether, our data shows that Epfn is crucial for the proper differentiation of dental mesenchymal cells towards functional odontoblasts and subsequent dentin-matrix deposition.

  3. Deficiency of G protein-coupled receptor Gpr111/Adgrf2 causes enamel hypomineralization in mice by alteration of the expression of kallikrein-related peptidase 4 (Klk4) during pH cycling process. International-journal Peer-reviewed

    Yuta Chiba, Keigo Yoshizaki, Hiroshi Sato, Tomoko Ikeuchi, Craig Rhodes, Mitsuki Chiba, Kan Saito, Takashi Nakamura, Tsutomu Iwamoto, Aya Yamada, Yoshihiko Yamada, Satoshi Fukumoto

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology　37　(4)　e22861　2023/04

    DOI： 10.1096/fj.202202053R 　

    ISSN： 0892-6638

    eISSN： 1530-6860

  4. GSK3beta inhibitor-induced dental mesenchymal stem cells regulate ameloblast differentiation. International-journal Peer-reviewed

    Aya Yamada, Keigo Yoshizaki, Kan Saito, Masaki Ishikawa, Yuta Chiba, Seira Hoshikawa, Mitsuki Chiba, Ryoko Hino, Yuriko Maruya, Hiroshi Sato, Keiji Masuda, Haruyoshi Yamaza, Takashi Nakamura, Tsutomu Iwamoto, Satoshi Fukumoto

    Journal of oral biosciences　64　(4)　400-409　2022/10/18

    DOI： 10.1016/j.job.2022.10.002 　

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    OBJECTIVES: Epithelial-mesenchymal interactions are extremely important in tooth development and essential for ameloblast differentiation, especially during tooth formation. We aimed to identify the type of mesenchymal cells important in ameloblast differentiation. METHODS: We used two types of cell culture systems with chambers and found that a subset of dental mesenchymal cells is important for the differentiation of dental epithelial cells into ameloblasts. Among odontogenic mesenchymal cells, dental pulp stem cell-like cells induced the expression of ameloblast differentiation markers. Therefore, we induced dental pulp stem cell-like cells from dental pulp stem cells using the small molecule compound BIO (a GSK-3 inhibitor IX) to clarify the mechanism involved in inducing ameloblast differentiation of dental pulp stem cells. RESULTS: The BIO-induced dental pulp cells promoted the expression of mesenchymal stem cell markers Oct3/4 and Bcrp1. Furthermore, we used artificial dental pulp stem cells induced by BIO to identify the molecules expressed in dental pulp stem cells required for ameloblast differentiation. Panx3 expression was induced in the dental pulp stem cell through interaction with the dental epithelial cells. In addition, ATP release from cells increased in Panx3-expressing cells. We also confirmed that ATP stimulation is accepted in dental epithelial cells. CONCLUSIONS: These results showed that the Panx3 expressed in dental pulp stem cells is important for ameloblast differentiation and that ATP release by Panx3 may play a role in epithelial-mesenchymal interaction.

  5. A parasympathetic neurotransmitter induces myoepithelial cell differentiation during salivary gland development Peer-reviewed

    Yuki Shindo, Hannah M. Nakamura, Junichi Nakai, Minoru Wakamori, Takashi Nakamura

    Experimental Cell Research　416　(1)　113137-113137　2022/07

    Publisher: Elsevier {BV}

    DOI： 10.1016/j.yexcr.2022.113137 　

    ISSN： 0014-4827

  6. Epiprofin Transcriptional Activation Promotes Ameloblast Induction From Mouse Induced Pluripotent Stem Cells via the BMP-Smad Signaling Axis Peer-reviewed

    Xinchao Miao, Kunimichi Niibe, Yunyu Fu, Maolin Zhang, Praphawi Nattasit, Yumi Ohori-Morita, Takashi Nakamura, Xinquan Jiang, Hiroshi Egusa

    Frontiers in Bioengineering and Biotechnology　10　2022/06/21

    Publisher: Frontiers Media SA

    DOI： 10.3389/fbioe.2022.890882 　

    eISSN： 2296-4185

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    The transcriptional regulation of induced pluripotent stem cells (iPSCs) holds promise for their directed differentiation into ameloblasts, which are usually lost after tooth eruption. Ameloblast differentiation is regulated by multiple signaling molecules, including bone morphogenetic proteins (BMPs). Epiprofin (Epfn), a transcription factor, is expressed in the dental epithelium, and epithelial Epfn overexpression results in ectopic ameloblast differentiation and enamel formation in mouse incisor, a striking phenotype resembling that of mice with deletion of follistatin (a BMP inhibitor). However, it remains unknown whether and how Epfn transcriptional activation promotes ameloblast induction from mouse iPSCs. Here, we generated doxycycline-inducible Epfn-expressing mouse iPSCs (Epfn-iPSCs). Ameloblasts, which are characterized by positive staining for keratin 14 and amelogenin and alizarin red S staining, were successfully derived from Epfn-iPSCs based on a stage-specific induction protocol, which involved the induction of the surface ectoderm, dental epithelial cells, and ameloblasts at stages 1, 2, and 3, respectively. Epfn activation by doxycycline at stages 2 and/or 3 decreased cell proliferation and promoted ameloblast differentiation, along with the upregulation of p-Smad1/5/8, a key regulator of the BMP-Smad signaling pathway. Gene analysis of the BMP-Smad signaling pathway-associated molecules revealed that Epfn activation decreased follistatin expression at stage 2, but increased BMP2/4/7 expression at stage 3. Perturbations in the ameloblast differentiation process were observed when the BMP-Smad signaling pathway was inhibited by a BMP receptor inhibitor (LDN-193189). Simultaneous LDN-193189 treatment and Epfn activation largely reversed the perturbations in ameloblast induction, with partial recovery of p-Smad1/5/8 expression, suggesting that Epfn activation promotes ameloblast induction from mouse iPSCs partially by upregulating BMP-Smad activity. These results reveal the potential regulatory networks between Epfn and the BMP-Smad pathway and suggest that Epfn is a promising target for inducing the differentiation of ameloblasts, which can be used in enamel and tooth regeneration.

  7. Sp6/Epiprofin is a master regulator in the developing tooth Peer-reviewed

    Craig S. Rhodes, Yasuo Yoshitomi, Peter D. Burbelo, Nowlan H. Freese, Takashi Nakamura, Yuta Chiba, Yoshihiko Yamada

    Biochemical and Biophysical Research Communications　581　89-95　2021/12/03

    DOI： 10.1016/j.bbrc.2021.10.017 　

    ISSN： 0006-291X

    eISSN： 1090-2104

  8. Stage-specific role of Amelx activation in stepwise ameloblast induction from mouse induced pluripotent stem cells Peer-reviewed

    Xinchao Miao, Kunimichi Niibe, Maolin Zhang, Zeni Liu, Praphawi Nattasit, Yumi Ohori-Morita, Takashi Nakamura, Xinquan Jiang, Hiroshi Egusa

    International Journal of Molecular Sciences　22　(13)　2021/07/01

    DOI： 10.3390/ijms22137195 　

    ISSN： 1661-6596

    eISSN： 1422-0067

  9. Pannexin 3 regulates skin development via Epiprofin. Peer-reviewed

    Takashi Nakamura

    Scientific reports　11　(1)　2021/01/19

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-021-81074-1 　

    eISSN： 2045-2322

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    <title>Abstract</title>Pannexin 3 (Panx3), a member of the gap junction pannexin family is required for the development of hard tissues including bone, cartilage and teeth. However, the role of Panx3 in skin development remains unclear. Here, we demonstrate that Panx3 regulates skin development by modulating the transcription factor, Epiprofin (Epfn). Panx3−/− mice have impaired skin development and delayed hair follicle regeneration. Loss of Panx3 in knockout mice and suppression by shRNA both elicited a reduction of Epfn expression in the epidermis. In cell culture, Panx3 overexpression promoted HaCaT cell differentiation, cell cycle exit and enhanced Epfn expression. Epfn−/− mice and inhibition of Epfn by siRNA showed no obvious differences of Panx3 expression. Furthermore, Panx3 promotes Akt/NFAT signaling pathway in keratinocyte differentiation by both Panx3 ATP releasing channel and ER Ca2+ channel functions. Our results reveal that Panx3 has a key role factor for the skin development by regulating Epfn.

  10. Connexin 43-Mediated Gap Junction Communication Regulates Ameloblast Differentiation via ERK1/2 Phosphorylation. International-journal Peer-reviewed

    Aya Yamada, Keigo Yoshizaki, Masaki Ishikawa, Kan Saito, Yuta Chiba, Emiko Fukumoto, Ryoko Hino, Seira Hoshikawa, Mitsuki Chiba, Takashi Nakamura, Tsutomu Iwamoto, Satoshi Fukumoto

    Frontiers in physiology　12　748574-748574　2021

    DOI： 10.3389/fphys.2021.748574 　

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    Connexin 43 (Cx43) is an integral membrane protein that forms gap junction channels. These channels mediate intercellular transport and intracellular signaling to regulate organogenesis. The human disease oculodentodigital dysplasia (ODDD) is caused by mutations in Cx43 and is characterized by skeletal, ocular, and dental abnormalities including amelogenesis imperfecta. To clarify the role of Cx43 in amelogenesis, we examined the expression and function of Cx43 in tooth development. Single-cell RNA-seq analysis and immunostaining showed that Cx43 is highly expressed in pre-secretory ameloblasts, differentiated ameloblasts, and odontoblasts. Further, we investigated the pathogenic mechanisms of ODDD by analyzing Cx43-null mice. These mice developed abnormal teeth with multiple dental epithelium layers. The expression of enamel matrix proteins such as ameloblastin (Ambn), which is critical for enamel formation, was significantly reduced in Cx43-null mice. TGF-β1 induces Ambn transcription in dental epithelial cells. The induction of Ambn expression by TGF-β1 depends on the density of the cultured cells. Cell culture at low densities reduces cell-cell contact and reduces the effect of TGF-β1 on Ambn induction. When cell density was high, Ambn expression by TGF-β1 was enhanced. This induction was inhibited by the gap junction inhibitors, oleamide, and 18α-grycyrrhizic acid and was also inhibited in cells expressing Cx43 mutations (R76S and R202H). TGF-β1-mediated phosphorylation and nuclear translocation of ERK1/2, but not Smad2/3, were suppressed by gap junction inhibitors. Cx43 gap junction activity is required for TGF-β1-mediated Runx2 phosphorylation through ERK1/2, which forms complexes with Smad2/3. In addition to its gap junction activity, Cx43 may also function as a Ca2+ channel that regulates slow Ca2+ influx and ERK1/2 phosphorylation. TGF-β1 transiently increases intracellular calcium levels, and the increase in intracellular calcium over a short period was not related to the expression level of Cx43. However, long-term intracellular calcium elevation was enhanced in cells overexpressing Cx43. Our results suggest that Cx43 regulates intercellular communication through gap junction activity by modulating TGF-β1-mediated ERK signaling and enamel formation.

  11. Erratum: Author Correction: Nrf2 contributes to the weight gain of mice during space travel (Communications biology (2020) 3 1 (496)) Peer-reviewed

    Takafumi Suzuki, Akira Uruno, Akane Yumoto, Keiko Taguchi, Mikiko Suzuki, Nobuhiko Harada, Rie Ryoke, Eriko Naganuma, Nanae Osanai, Aya Goto, Hiromi Suda, Ryan Browne, Akihito Otsuki, Fumiki Katsuoka, Michael Zorzi, Takahiro Yamazaki, Daisuke Saigusa, Seizo Koshiba, Takashi Nakamura, Satoshi Fukumoto, Hironobu Ikehata, Keizo Nishikawa, Norio Suzuki, Ikuo Hirano, Ritsuko Shimizu, Tetsuya Oishi, Hozumi Motohashi, Hirona Tsubouchi, Risa Okada, Takashi Kudo, Michihiko Shimomura, Thomas W. Kensler, Hiroyasu Mizuno, Masaki Shirakawa, Satoru Takahashi, Dai Shiba, Masayuki Yamamoto

    Communications biology　3　(1)　566　2020/10/07

    DOI： 10.1038/s42003-020-01292-7 　

    eISSN： 2399-3642

  12. Nrf2 contributes to the weight gain of mice during space travel. International-journal Peer-reviewed

    Takafumi Suzuki, Akira Uruno, Akane Yumoto, Keiko Taguchi, Mikiko Suzuki, Nobuhiko Harada, Rie Ryoke, Eriko Naganuma, Nanae Osanai, Aya Goto, Hiromi Suda, Ryan Browne, Akihito Otsuki, Fumiki Katsuoka, Michael Zorzi, Takahiro Yamazaki, Daisuke Saigusa, Seizo Koshiba, Takashi Nakamura, Satoshi Fukumoto, Hironobu Ikehata, Keizo Nishikawa, Norio Suzuki, Ikuo Hirano, Ritsuko Shimizu, Tetsuya Oishi, Hozumi Motohashi, Hirona Tsubouchi, Risa Okada, Takashi Kudo, Michihiko Shimomura, Thomas W Kensler, Hiroyasu Mizuno, Masaki Shirakawa, Satoru Takahashi, Dai Shiba, Masayuki Yamamoto

    Communications biology　3　(1)　496-496　2020/09/08

    DOI： 10.1038/s42003-020-01227-2 　

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    Space flight produces an extreme environment with unique stressors, but little is known about how our body responds to these stresses. While there are many intractable limitations for in-flight space research, some can be overcome by utilizing gene knockout-disease model mice. Here, we report how deletion of Nrf2, a master regulator of stress defense pathways, affects the health of mice transported for a stay in the International Space Station (ISS). After 31 days in the ISS, all flight mice returned safely to Earth. Transcriptome and metabolome analyses revealed that the stresses of space travel evoked ageing-like changes of plasma metabolites and activated the Nrf2 signaling pathway. Especially, Nrf2 was found to be important for maintaining homeostasis of white adipose tissues. This study opens approaches for future space research utilizing murine gene knockout-disease models, and provides insights into mitigating space-induced stresses that limit the further exploration of space by humans.

  13. G-protein coupled receptor Gpr115 (Adgrf4) is required for enamel mineralization mediated by ameloblasts. International-journal Peer-reviewed

    Yuta Chiba, Keigo Yoshizaki, Kan Saito, Tomoko Ikeuchi, Tsutomu Iwamoto, Craig Rhodes, Takashi Nakamura, Susana de Vega, Robert J Morell, Erich T Boger, Daniel Martin, Ryoko Hino, Hiroyuki Inuzuka, Christopher K E Bleck, Aya Yamada, Yoshihiko Yamada, Satoshi Fukumoto

    The Journal of biological chemistry　295　(45)　15328-15341　2020/08/31

    DOI： 10.1074/jbc.RA120.014281 　

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    Dental enamel, the hardest tissue in the human body, is derived from dental epithelial cell ameloblast-secreted enamel matrices. Enamel mineralization occurs in a strictly synchronized manner along with ameloblast maturation in association with ion transport and pH balance, and any disruption of these processes results in enamel hypomineralization. G-protein coupled receptors (GPCRs) function as transducers of external signals by activating associated G-proteins and regulate cellular physiology. Tissue-specific GPCRs play important roles in organ development, though their activities in tooth development remains poorly understood. The present results show that the adhesion-GPCR Gpr115 (Adgrf4) is highly and preferentially expressed in mature ameloblasts and plays a crucial role during enamel mineralization. To investigate the in vivo function of Gpr115, knockout (Gpr115-KO) mice were created and found to develop hypo-mineralized enamel, with a larger acidic area due to the dysregulation of ion composition. Transcriptomic analysis also revealed that deletion of Gpr115 disrupted pH homeostasis and ion transport processes in enamel formation. In addition, in vitroanalyses using the dental epithelial cell line Cervical Loop-Derived Dental Epithelial (CLDE) cell demonstrated that Gpr115 is indispensable for the expression of carbonic anhydrase 6 (Car6), which has a critical role in enamel mineralization. Furthermore, an acidic condition induced Car6 expression under the regulation of Gpr115 in CLDE cells. Thus, we concluded that Gpr115 plays an important role in enamel mineralization via regulation of Car6 expression in ameloblasts. The present findings indicate a novel function of Gpr115 in ectodermal organ development and clarify the molecular mechanism of enamel formation.

  14. Sox21 Regulates Anapc10 Expression and Determines the Fate of Ectodermal Organ Peer-reviewed

    Kan Saito, Frederic Michon, Aya Yamada, Hiroyuki Inuzuka, Satoko Yamaguchi, Emiko Fukumoto, Keigo Yoshizaki, Takashi Nakamura, Makiko Arakaki, Yuta Chiba, Masaki Ishikawa, Hideyuki Okano, Irma Thesleff, Satoshi Fukumoto

    iScience　23　(7)　101329-101329　2020/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.isci.2020.101329 　

    ISSN： 2589-0042

  15. Paeonol, an ingredient of kamishoyosan, reduces intracellular lipid accumulation by inhibiting glucocorticoid receptor activity in 3T3-L1 cells Peer-reviewed

    Masayuki Izumi, Takashi Yoshida, Takashi Nakamura, Minoru Wakamori

    Nutrients　12　(2)　2020/02

    DOI： 10.3390/nu12020309 　

    eISSN： 2072-6643

  16. Single-Cell RNA-Sequencing From Mouse Incisor Reveals Dental Epithelial Cell-Type Specific Genes. International-journal Peer-reviewed

    Yuta Chiba, Kan Saito, Daniel Martin, Erich T Boger, Craig Rhodes, Keigo Yoshizaki, Takashi Nakamura, Aya Yamada, Robert J Morell, Yoshihiko Yamada, Satoshi Fukumoto

    Frontiers in cell and developmental biology　8　841-841　2020

    DOI： 10.3389/fcell.2020.00841 　

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    Dental epithelial stem cells give rise to four types of dental epithelial cells: inner enamel epithelium (IEE), outer enamel epithelium (OEE), stratum intermedium (SI), and stellate reticulum (SR). IEE cells further differentiate into enamel-forming ameloblasts, which play distinct roles, and are essential for enamel formation. These are conventionally classified by their shape, although their transcriptome and biological roles are yet to be fully understood. Here, we aimed to use single-cell RNA sequencing to clarify the heterogeneity of dental epithelial cell types. Unbiased clustering of 6,260 single cells from incisors of postnatal day 7 mice classified them into two clusters of ameloblast, IEE/OEE, SI/SR, and two mesenchymal populations. Secretory-stage ameloblasts expressed Amel and Enam were divided into Dspp + and Ambn + ameloblasts. Pseudo-time analysis indicated Dspp + ameloblasts differentiate into Ambn + ameloblasts. Further, Dspp and Ambn could be stage-specific markers of ameloblasts. Gene ontology analysis of each cluster indicated potent roles of cell types: OEE in the regulation of tooth size and SR in the transport of nutrients. Subsequently, we identified novel dental epithelial cell marker genes, namely Pttg1, Atf3, Cldn10, and Krt15. The results not only provided a resource of transcriptome data in dental cells but also contributed to the molecular analyses of enamel formation.

  17. Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells. International-journal Peer-reviewed

    Tomoaki Nakamura, Tsutomu Iwamoto, Hannah M Nakamura, Yuki Shindo, Kan Saito, Aya Yamada, Yoshihiko Yamada, Satoshi Fukumoto, Takashi Nakamura

    Frontiers in cell and developmental biology　8　156-156　2020

    DOI： 10.3389/fcell.2020.00156 　

    eISSN： 2296-634X

  18. The transcription factor AmeloD stimulates epithelial cell motility essential for tooth morphology. International-journal Peer-reviewed

    Yuta Chiba, Bing He, Keigo Yoshizaki, Craig Rhodes, Muneaki Ishijima, Christopher K E Bleck, Erin Stempinski, Emily Y Chu, Takashi Nakamura, Tsutomu Iwamoto, Susana de Vega, Kan Saito, Satoshi Fukumoto, Yoshihiko Yamada

    The Journal of biological chemistry　294　(10)　3406-3418　2019/03/08

    DOI： 10.1074/jbc.RA118.005298 　

    ISSN： 1083-351X

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    The development of ectodermal organs, such as teeth, requires epithelial-mesenchymal interactions. Basic helix-loop-helix (bHLH) transcription factors regulate various aspects of tissue development, and we have previously identified a bHLH transcription factor, AmeloD, from a tooth germ cDNA library. Here, we provide both in vitro and in vivo evidence that AmeloD is important in tooth development. We created AmeloD-knockout (KO) mice to identify the in vivo functions of AmeloD that are critical for tooth morphogenesis. We found that AmeloD-KO mice developed enamel hypoplasia and small teeth because of increased expression of E-cadherin in inner enamel epithelial (IEE) cells, and it may cause inhibition of the cell migration. We used the CLDE dental epithelial cell line to conduct further mechanistic analyses to determine whether AmeloD overexpression in CLDE cells suppresses E-cadherin expression and promotes cell migration. Knockout of epiprofin (Epfn), another transcription factor required for tooth morphogenesis and development, and analysis of AmeloD expression and deletion revealed that AmeloD also contributed to multiple tooth formation in Epfn-KO mice by promoting the invasion of dental epithelial cells into the mesenchymal region. Thus, AmeloD appears to play an important role in tooth morphogenesis by modulating E-cadherin and dental epithelial-mesenchymal interactions. These findings provide detailed insights into the mechanism of ectodermal organ development.

  19. Patients with SATB2-associated syndrome exhibiting multiple odontomas Peer-reviewed

    Takashi Kikuiri, Hiroyuki Mishima, Hideto Imura, Satoshi Suzuki, Yusuke Matsuzawa, Takashi Nakamura, Satoshi Fukumoto, Yoshitaka Yoshimura, Satoshi Watanabe, Akira Kinoshita, Takahiro Yamada, Masanobu Shindoh, Yoshihiko Sugita, Hatsuhiko Maeda, Yasutaka Yawaka, Tadashi Mikoya, Nagato Natsume, Koh ichiro Yoshiura

    American Journal of Medical Genetics, Part A　176　(12)　2614-2622　2018/12

    DOI： 10.1002/ajmg.a.40670 　

    ISSN： 1552-4825

    eISSN： 1552-4833

  20. Cyclic stretch negatively regulates IL-1β secretion through the inhibition of NLRP3 inflammasome activation by attenuating the AMP kinase pathway Peer-reviewed

    Kentaro Maruyama, Yukihiko Sakisaka, Mizuki Suto, Hiroyuki Tada, Takashi Nakamura, Satoru Yamada, Eiji Nemoto

    Frontiers in Physiology　9　(JUN)　802　2018/06/28

    DOI： 10.3389/fphys.2018.00802 　

    eISSN： 1664-042X

  21. Pannexin 3 regulates proliferation and differentiation of odontoblasts via its hemichannel activities Peer-reviewed

    Tsutomu Iwamoto, Takashi Nakamura, Masaki Ishikawa, Keigo Yoshizaki, Asuna Sugimoto, Hiroko Ida-Yonemochi, Hayato Ohshima, Masahiro Saito, Yoshihiko Yamada, Satoshi Fukumoto

    PLoS ONE　12　(5)　2017/05

    DOI： 10.1371/journal.pone.0177557 　

    ISSN： 1932-6203

    eISSN： 1932-6203

  22. Epiprofin Regulates Enamel Formation and Tooth Morphogenesis by Controlling Epithelial-Mesenchymal Interactions During Tooth Development Peer-reviewed

    Takashi Nakamura, Lucia Jimenez-Rojo, Eiki Koyama, Maurizio Pacifici, Susana de Vega, Masahiro Iwamoto, Satoshi Fukumoto, Fernando Unda, Yoshihiko Yamada

    Journal of Bone and Mineral Research　32　(3)　601-610　2017/03/01

    DOI： 10.1002/jbmr.3024 　

    ISSN： 0884-0431

    eISSN： 1523-4681

  23. The effect of three-dimensional culture on the ameloblastic differentiation of dental epithelial cells Peer-reviewed

    Takahisa Anada, Mayu Tadaki, Yukari Shiwaku, Takashi Nakamura, Masanori Nakamura, Masaru Kojima, Tatsuo Arai, Satoshi Fukumoto, Osamu Suzuki

    2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016　2017/01/18

    DOI： 10.1109/MHS.2016.7824173 　

    ISSN： 2474-378X

    eISSN： 2474-3798

  24. Globoside accelerates the differentiation of dental epithelial cells into ameloblasts Peer-reviewed

    Takashi Nakamura, Yuta Chiba, Masahiro Naruse, Kan Saito, Hidemitsu Harada, Satoshi Fukumoto

    International Journal of Oral Science　8　(4)　205-212　2016/12/16

    DOI： 10.1038/ijos.2016.35 　

    ISSN： 1674-2818

    eISSN： 2049-3169

  25. Fine tuning of Rac1 and RhoA alters cuspal shapes by remolding the cellular geometry Peer-reviewed

    Liwen Li, Qinghuang Tang, Takashi Nakamura, Jun Gyo Suh, Hayato Ohshima, Han Sung Jung

    Scientific Reports　6　2016/11/28

    DOI： 10.1038/srep37828 　

    ISSN： 2045-2322

    eISSN： 2045-2322

  26. p38 MAP kinase is required for Wnt3a-mediated osterix expression independently of Wnt-LRP5/6-GSK3β signaling axis in dental follicle cells Peer-reviewed

    Yukihiko Sakisaka, Sousuke Kanaya, Takashi Nakamura, Masato Tamura, Hidetoshi Shimauchi, Eiji Nemoto

    Biochemical and Biophysical Research Communications　478　(2)　527-532　2016/09/16

    DOI： 10.1016/j.bbrc.2016.07.076 　

    ISSN： 0006-291X

    eISSN： 1090-2104

  27. Wnt3a signaling induces murine dental follicle cells to differentiate into cementoblastic/osteoblastic cells via an osterix-dependent pathway Peer-reviewed

    E. Nemoto, Y. Sakisaka, M. Tsuchiya, M. Tamura, T. Nakamura, S. Kanaya, M. Shimonishi, H. Shimauchi

    Journal of Periodontal Research　51　(2)　164-174　2016/04

    DOI： 10.1111/jre.12294 　

    ISSN： 0022-3484

    eISSN： 1600-0765

  28. Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation Peer-reviewed

    Jia Liu, Kan Saito, Yuriko Maruya, Takashi Nakamura, Aya Yamada, Emiko Fukumoto, Momoko Ishikawa, Tsutomu Iwamoto, Kanako Miyazaki, Keigo Yoshizaki, Lihong Ge, Satoshi Fukumoto

    Scientific Reports　6　2016/03/31

    DOI： 10.1038/srep23670 　

    ISSN： 2045-2322

    eISSN： 2045-2322

  29. Wnt/β-Catenin regulates the activity of Epiprofin/Sp6, SHH, FGF, and BMP to coordinate the stages of odontogenesis Peer-reviewed

    Maitane Aurrekoetxea, Igor Irastorza, Patricia García-Gallastegui, Lucia Jiménez-Rojo, Takashi Nakamura, Yoshihiko Yamada, Gaskon Ibarretxe, Fernando J. Unda

    Frontiers in Cell and Developmental Biology　4　(MAR)　2016/03/30

    Publisher: Frontiers Media SA

    DOI： 10.3389/fcell.2016.00025 　

    eISSN： 2296-634X

  30. Connexin 43 is necessary for salivary gland branching morphogenesis and FGF10-induced ERK1/2 phosphorylation Peer-reviewed

    Aya Yamada, Masaharu Futagi, Emiko Fukumoto, Kan Saito, Keigo Yoshizaki, Masaki Ishikawa, Makiko Arakaki, Ryoko Hino, Yu Sugawara, Momoko Ishikawa, Masahiro Naruse, Kanako Miyazaki, Takashi Nakamura, Satoshi Fukumoto

    Journal of Biological Chemistry　291　(2)　904-912　2016/01/08

    DOI： 10.1074/jbc.M115.674663 　

    ISSN： 0021-9258

    eISSN： 1083-351X

  31. A 3D culture model study monitoring differentiation of dental epithelial cells into ameloblast-like cells Peer-reviewed

    Mayu Tadaki, Takahisa Anada, Yukari Shiwaku, Takashi Nakamura, Masanori Nakamura, Masaru Kojima, Tatsuo Arai, Satoshi Fukumoto, Osamu Suzuki

    RSC Advances　6　(67)　62109-62118　2016

    DOI： 10.1039/c6ra04570g 　

    ISSN： 2046-2069

    eISSN： 2046-2069

  32. Articular cartilage endurance and resistance to osteoarthritic changes require transcription factor Erg Peer-reviewed

    Yoichi Ohta, Takahiro Okabe, Colleen Larmour, Agnese Di Rocco, Marijke W. Maijenburg, Amanda Phillips, Nancy A. Speck, Shigeyuki Wakitani, Takashi Nakamura, Yoshihiko Yamada, Motomi Enomoto-Iwamoto, Maurizio Pacifici, Masahiro Iwamoto

    Arthritis &amp; rheumatology (Hoboken, N.J.)　67　(10)　2679-2690　2015/10/01

    DOI： 10.1002/art.39243 　

    ISSN： 2326-5191

    eISSN： 2326-5205

  33. Interaction between fibronectin and β1integrin is essential for tooth development Peer-reviewed

    Kan Saito, Emiko Fukumoto, Aya Yamada, Kenji Yuasa, Keigo Yoshizaki, Tsutomu Iwamoto, Masahiro Saito, Takashi Nakamura, Satoshi Fukumoto

    PLoS ONE　10　(4)　2015/04

    DOI： 10.1371/journal.pone.0121667 　

    ISSN： 1932-6203

    eISSN： 1932-6203

  34. 白血病治療を目指したヒト乳歯歯髄細胞から人工骨髄誘導法の開発

    山田 亜矢, 菊入 崇, 中村 卓史, 新垣 真紀子, 齋藤 幹, 福本 敏

    小児歯科学雑誌　53　(2)　220-220　2015/04

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  35. Novel hedgehog agonists promote osteoblast differentiation in mesenchymal stem cells Peer-reviewed

    Takashi Nakamura, Masahiro Naruse, Yuta Chiba, Toshihisa Komori, Keiichi Sasaki, Masahiro Iwamoto, Satoshi Fukumoto

    Journal of Cellular Physiology　230　(4)　922-929　2015/04

    DOI： 10.1002/jcp.24823 　

    ISSN： 0021-9541

    eISSN： 1097-4652

  36. Wnt5a attenuates Wnt3a-induced alkaline phosphatase expression in dental follicle cells Peer-reviewed

    Yukihiko Sakisaka, Masahiro Tsuchiya, Takashi Nakamura, Masato Tamura, Hidetoshi Shimauchi, Eiji Nemoto

    Experimental Cell Research　336　(1)　85-93　2015

    DOI： 10.1016/j.yexcr.2015.06.013 　

    ISSN： 0014-4827

    eISSN： 1090-2422

  37. Epiprofin orchestrates epidermal keratinocyte proliferation and differentiation Peer-reviewed

    Takashi Nakamura, Yasuo Yoshitomi, Kiyoshi Sakai, Vyomesh Patel, Satoshi Fukumoto, Yoshihiko Yamada

    JOURNAL OF CELL SCIENCE　127　(24)　5261-5272　2014/12

    DOI： 10.1242/jcs.156778 　

    ISSN： 0021-9533

    eISSN： 1477-9137

  38. 歯根と歯周組織発生の分子機構解明の新たなる展開と歯科疾患へのアプローチ 歯根発生過程における細胞骨格制御因子の役割とその異常

    福本 敏, 日野 綾子, 山田 亜矢, 大津 圭史, 新垣 真紀子, 齋藤 幹, 中村 卓史, 原田 英光

    Journal of Oral Biosciences Supplement　2014　80-80　2014/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  39. New insights into the functions of enamel matrices in calcified tissues

    Satoshi Fukumoto, Takashi Nakamura, Aya Yamada, Makiko Arakaki, Kan Saito, Juan Xu, Emiko Fukumoto, Yoshihiko Yamada

    Japanese Dental Science Review　50　(2)　47-54　2014

    Publisher: Elsevier BV

    DOI： 10.1016/j.jdsr.2014.01.001 　

    ISSN： 1882-7616

  40. Establishment of ex vivo mucocele model using salivary gland organ culture Peer-reviewed

    Ryoko Hino, Masaharu Futagi, Aya Yamada, Makiko Arakaki, Kan Saito, Yu Sugawara, Mariko Ono, Emiko Fukumoto, Takashi Nakamura, Satoshi Fukumoto

    Pediatric Dental Journal　24　(2)　78-82　2014

    Publisher: Elsevier Ltd

    DOI： 10.1016/j.pdj.2014.03.003 　

    ISSN： 1880-3997 0917-2394

    eISSN： 1880-3997

  41. Biological roles of gap junction proteins in cartilage and bone development

    Tsutomu Iwamoto, Masaki Ishikawa, Mariko Ono, Takashi Nakamura, Satoshi Fukumoto, Yoshihiko Yamada

    Journal of Oral Biosciences　55　(1)　29-33　2013

    Publisher: Japanese Association for Oral Biology

    DOI： 10.1016/j.job.2012.12.001 　

    ISSN： 1349-0079

  42. Genetics of supernumerary tooth formation

    Takashi Nakamura, Satoshi Fukumoto

    Journal of Oral Biosciences　55　(4)　180-183　2013

    Publisher: Japanese Association for Oral Biology

    DOI： 10.1016/j.job.2013.06.006 　

    ISSN： 1349-0079

  43. A vitamin D-3 analog augmented interleukin-8 production by human monocytic cells in response to various microbe-related synthetic ligands, especially NOD2 agonistic muramyldipeptide Peer-reviewed

    Tomoko Ikeuchi, Takashi Nakamura, Satoshi Fukumoto, Haruhiko Takada

    INTERNATIONAL IMMUNOPHARMACOLOGY　15　(1)　15-22　2013/01

    DOI： 10.1016/j.intimp.2012.10.027 　

    ISSN： 1567-5769

  44. Epiprofin/Sp6 regulates Wnt-BMP signaling and the establishment of cellular junctions during the bell stage of tooth development Peer-reviewed

    Gaskon Ibarretxe, Maitane Aurrekoetxea, Olatz Crende, Iker Badiola, Lucia Jimenez-Rojo, Takashi Nakamura, Yoshihiko Yamada, Fernando Unda

    CELL AND TISSUE RESEARCH　350　(1)　95-107　2012/10

    DOI： 10.1007/s00441-012-1459-8 　

    ISSN： 0302-766X

  45. Wnt5a signaling is a substantial constituent in bone morphogenetic protein-2-mediated osteoblastogenesis Peer-reviewed

    Eiji Nemoto, Yukari Ebe, Sousuke Kanaya, Masahiro Tsuchiya, Takashi Nakamura, Masato Tamura, Hidetoshi Shimauchi

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　422　(4)　627-632　2012/06

    DOI： 10.1016/j.bbrc.2012.05.039 　

    ISSN： 0006-291X

  46. Role of Epithelial-Stem Cell Interactions during Dental Cell Differentiation Peer-reviewed

    Makiko Arakaki, Masaki Ishikawa, Takashi Nakamura, Tsutomu Iwamoto, Aya Yamada, Emiko Fukumoto, Masahiro Saito, Keishi Otsu, Hidemitsu Harada, Yoshihiko Yamada, Satoshi Fukumoto

    JOURNAL OF BIOLOGICAL CHEMISTRY　287　(13)　10590-10601　2012/03

    DOI： 10.1074/jbc.M111.285874 　

    ISSN： 0021-9258

    eISSN： 1083-351X

  47. Epithelial-mesenchymal interaction reduces inhibitory effects of fluoride on proliferation and enamel matrix expression in dental epithelial cells

    Aya Yamada, Tsutomu Iwamoto, Emiko Fukumoto, Makiko Arakaki, Ryoko Miyamoto, Yu Sugawara, Hideji Komatsu, Takashi Nakamura, Satoshi Fukumoto

    Pediatric Dental Journal　22　(1)　55-63　2012

    DOI： 10.11411/pdj.22.55 　

    ISSN： 1880-3997 0917-2394

    eISSN： 1880-3997

  48. Epithelial cell lines in the field of dental research: Review

    Satoshi Fukumoto, Makiko Arakaki, Tsutomu Iwamoto, Aya Yamada, Ryoko Miyamoto, Masahiro Naruse, Takashi Nakamura

    Interface Oral Health Science 2011　327-333　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_97 　

  49. Review: The regulation of tooth development and morphogenesis

    Takashi Nakamura, Yoshihiko Yamada, Satoshi Fukumoto

    Interface Oral Health Science 2011　14-21　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_3 　

  50. Pannexin 3, a gap junction protein, regulates chondrocyte differentiation in part through hemichannel activity

    Tsutomu Iwamoto, Mariko Ono, Makiko Arakaki, Takashi Nakamura, Aya Yamada, Satoshi Fukumoto

    Interface Oral Health Science 2011　346-348　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_100 　

  51. Glycosphingolipids Regulate Ameloblastin Expression in Dental Epithelial Cells Peer-reviewed

    Y. Kamasaki, T. Nakamura, K. Yoshizaki, T. Iwamoto, A. Yamada, E. Fukumoto, Y. Maruya, K. Iwabuchi, K. Furukawa, T. Fujiwara, S. Fukumoto

    JOURNAL OF DENTAL RESEARCH　91　(1)　78-83　2012/01

    DOI： 10.1177/0022034511424408 　

    ISSN： 0022-0345

  52. Diverse Function of Epiprofin in Tooth Development

    Takashi Nakamura, Satoshi Fukumoto, Yoshihiko Yamada

    Journal of Oral Biosciences　53　(1)　22-30　2011/09/14

    DOI： 10.1016/S1349-0079(11)80032-0 　

  53. Pannexin 3 functions as an ER Ca2+ channel, hemichannel, and gap junction to promote osteoblast differentiation Peer-reviewed

    Masaki Ishikawa, Tsutomu Iwamoto, Takashi Nakamura, Andrew Doyle, Satoshi Fukumoto, Yoshihiko Yamada

    JOURNAL OF CELL BIOLOGY　193　(7)　1257-1274　2011/06

    DOI： 10.1083/jcb.201101050 　

    ISSN： 0021-9525

  54. Expression and Functional Roles of Pannexin 3 in Odontoblast Proliferation and Differentiation Peer-reviewed

    Tsutomu Iwamoto, Mariko Ono, Takashi Nakamura, Aya Yamada, Yoshihiko Yamada, Satoshi Fukumoto

    FASEB JOURNAL　25　2011/04

    ISSN： 0892-6638

  55. Diverse functions of Epiprofin in ectodermal organogenesis Peer-reviewed

    Takashi Nakamura, Satoshi Fukumoto, Yoshihiko Yamada

    FASEB JOURNAL　25　2011/04

    ISSN： 0892-6638

  56. Diverse function of epiprofin in tooth development

    Takashi Nakamura, Satoshi Fukumoto, Yoshihiko Yamada

    Journal of Oral Biosciences　53　(1)　22-30　2011

    Publisher: Japanese Association for Oral Biology

    DOI： 10.2330/joralbiosci.53.22 　

    ISSN： 1349-0079

  57. Expressions and functions of neurotrophic factors in tooth development

    Tsutomu Iwamoto, Aya Yamada, Makiko Arakaki, Yu Sugawara, Mariko Ono, Masaharu Futaki, Keigo Yoshizaki, Emiko Fukumoto, Takashi Nakamura, Satoshi Fukumoto

    Journal of Oral Biosciences　53　(1)　13-21　2011

    Publisher: Japanese Association for Oral Biology

    DOI： 10.2330/joralbiosci.53.13 　

    ISSN： 1349-0079

  58. Expressions and functions of neurotrophic factors in tooth development

    Tsutomu Iwamoto, Aya Yamada, Makiko Arakaki, Yu Sugawara, Mariko Ono, Masaharu Futaki, Keigo Yoshizaki, Emiko Fukumoto, Takashi Nakamura, Satoshi Fukumoto

    Journal of Oral Biosciences　53　(1)　13-21　2011

    Publisher: Japanese Association for Oral Biology

    DOI： 10.2330/joralbiosci.53.13 　

    ISSN： 1349-0079

  59. Epiprofin/Sp6: A new player in the regulation of tooth development Peer-reviewed

    Lucia Jimenez-Rojo, Gaskon Ibarretxe, Maitane Aurrekoetxea, Susana de Vega, Takashi Nakamura, Yoshihiko Yamada, Fernando Unda

    HISTOLOGY AND HISTOPATHOLOGY　25　(12)　1621-1630　2010/12

    ISSN： 0213-3911

  60. Epiprofin/Sp6: A new player in the regulation of tooth development Peer-reviewed

    Lucia Jimenez-Rojo, Gaskon Ibarretxe, Maitane Aurrekoetxea, Susana de Vega, Takashi Nakamura, Yoshihiko Yamada, Fernando Unda

    HISTOLOGY AND HISTOPATHOLOGY　25　(12)　1621-1630　2010/12

    ISSN： 0213-3911

  61. 【知っておきたい「小児歯科」】 小児歯科で取り組む再生医療 Invited

    福本敏, 山田亜矢, 岩本勉, 中村卓史

    小児科臨床　63　(11)　2303-2307　2010/11

    Publisher:

    ISSN： 0021-518X

  62. 象牙芽細胞分化におけるギャップ結合分子の発現および機能解析

    岩本勉, 中村卓史, 吉崎恵吾, 山田亜矢, 福本敏

    Journal of Oral Biosciences　52　(Suppl)　154-154　2010/09

    Publisher:

    ISSN： 1349-0079

    eISSN： 1880-3865

  63. 神経栄養因子による歯原性上皮細胞の増殖、分化制御機構の解明

    小野真理子, 岩本勉, 吉崎恵吾, 中村卓史, 山田亜矢, 宮本綾子, 福本敏

    Journal of Oral Biosciences　52　(Suppl)　134-134　2010/09

    Publisher:

    ISSN： 1349-0079

    eISSN： 1880-3865

  64. インテグリン-フィラミンA複合体による歯の形態形成の分子制御

    宮本綾子, 山田亜矢, 中村卓史, 岩本勉, 小野真理子, 福本敏

    Journal of Oral Biosciences　52　(Suppl)　127-127　2010/09

    Publisher:

    ISSN： 1349-0079

    eISSN： 1880-3865

  65. 歯科再生医療に歯の発生生物学はどのように貢献してきたか、そして今後どのように貢献できるか 歯原性上皮の分化の分子制御メカニズム Invited

    福本敏, 中村卓史, 山田亜矢, 新垣真紀子, 岩本勉

    Journal of Oral Biosciences　52　(Suppl)　71-71　2010/09

    Publisher:

    ISSN： 1349-0079

    eISSN： 1880-3865

  66. 器官形成における転写因子エピプロフィンの機能

    中村卓史, 岩本勉, 山田亜矢, 山田吉彦, 福本敏

    東北大学歯学雑誌　29　(1)　31-32　2010/06

    Publisher:

    ISSN： 0287-3915

  67. エナメル芽細胞の分化制御機構

    山田亜矢, 岩本勉, 中村卓史, 福本敏

    小児歯科学雑誌　48　(3)　374-380　2010/06

    Publisher: Japanese Society of Pediatric Dentistry

    DOI： 10.11411/jspd.48.3_374 　

    ISSN： 0583-1199

    eISSN： 2186-5078

    More details Close

    Tooth organs develop through the sequential reciprocal signaling interaction between ectodermalderived epithelial cells and cranial neural crest-derived mesenchymal cells. In recent advances in tissue engineering technology and developmental biology, a partial tooth regeneration has been demonstrated by using embryonic mouse dental epithelial and mesenchyme cells. However, no technology of tooth reconstruction has been identified to regulate tooth morphogenesis possessing positional characters of original tooth shape and size for its function. In this study, we focus on the roles of ameloblastin, one of the enamel matrix proteins, in dental epithelial cell differentiation into ameloblast and enamel formation. Ameloblastin regulates not only ameloblast proliferation and differentiation,but also enamel formation. In addition, ameloblastin controls the cell polarity of ameloblast, which go through multiple stages of differentiation to orchestrate enamel formation. The enamel epithelium does not exist in adult teeth, and currently there are not readily available sources of dental epithelial cells. This limitation, along with a lack of knowledge on mechanisms guiding the formation of enamel, impacts our capacity to develop strategies for the repair and regeneration of enamel.

  68. PDGFs regulate tooth germ proliferation and ameloblast differentiation Peer-reviewed

    Nan Wu, Tsutomu Iwamoto, Yu Sugawara, Masaharu Futaki, Keigo Yoshizaki, Shinya Yamamoto, Aya Yamada, Takashi Nakamura, Kazuaki Nonaka, Satoshi Fukumoto

    ARCHIVES OF ORAL BIOLOGY　55　(6)　426-434　2010/06

    DOI： 10.1016/j.archoralbio.2010.03.011 　

    ISSN： 0003-9969

  69. Pannexin 3 Regulates Intracellular ATP/cAMP Levels and Promotes Chondrocyte Differentiation Peer-reviewed

    Tsutomu Iwamoto, Takashi Nakamura, Andrew Doyle, Masaki Ishikawa, Susana de Vega, Satoshi Fukumoto, Yoshihiko Yamada

    JOURNAL OF BIOLOGICAL CHEMISTRY　285　(24)　18948-18958　2010/06

    DOI： 10.1074/jbc.M110.127027 　

    ISSN： 0021-9258

  70. エピプロフィンによる歯原性上皮細胞の増殖と分化の制御機構

    中村卓史, 岩本勉, 山田亜矢, 福本敏

    小児歯科学雑誌　48　(2)　247-247　2010/04

    Publisher:

    ISSN： 0583-1199

    eISSN： 2186-5078

  71. 唾液腺分岐におけるギャップ結合の役割解明と再生への応用

    鈴木宏治, 山田亜矢, 岩本勉, 中村卓史, 福本敏

    小児歯科学雑誌　48　(2)　238-238　2010/04

    Publisher:

    ISSN： 0583-1199

    eISSN： 2186-5078

  72. Pannexin 3, a gap junction protein, Regulates Odontoblasts Differentiation Peer-reviewed

    Tsutomu Iwamoto, Takashi Nakamura, Aya Yamada, Yoshihiko Yamada, Satoshi Fukumoto

    FASEB JOURNAL　24　2010/04

    ISSN： 0892-6638

  73. Essential roles of zinc finger factor epiprofin in tooth development Peer-reviewed

    Takashi Nakamura, Kenji Yuasa, Lucia Jimenez, Fernando Unda, Eiki Koyama, Satoshi Fukumoto, Yoshihiko Yamada

    FASEB JOURNAL　24　2010/04

    ISSN： 0892-6638

  74. 顎顔面発生研究の新規展開 若手研究者の発想とねらい 上皮器官形成における転写因子エピプロフィンの役割 Invited

    中村卓史, 山田吉彦, 福本敏

    解剖学雑誌　85　(Suppl.)　96-96　2010/03

    Publisher:

    ISSN： 0022-7722

  75. Over-expression of thymosin beta 4 promotes abnormal tooth development and stimulation of hair growth Peer-reviewed

    Hee-Jae Cha, Deborah Philp, Soo-Hyun Lee, Hye-Sung Moon, Hynda K. Kleinman, Takashi Nakamura

    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY　54　(1)　135-140　2010

    DOI： 10.1387/ijdb.082735hc 　

    ISSN： 0214-6282

  76. Role of Epiprofin, a zinc-finger transcription factor, in limb development Peer-reviewed

    Ana Talamillo, Irene Delgado, Takashi Nakamura, Susana de-Vega, Yasuo Yoshitomi, Fernando Unda, Walter Birchmeier, Yoshihiko Yamada, Maria A. Ros

    DEVELOPMENTAL BIOLOGY　337　(2)　363-374　2010/01

    DOI： 10.1016/j.ydbio.2009.11.007 　

    ISSN： 0012-1606

  77. Over-expression of thymosin beta 4 promotes abnormal tooth development and stimulation of hair growth Peer-reviewed

    Hee-Jae Cha, Deborah Philp, Soo-Hyun Lee, Hye-Sung Moon, Hynda K. Kleinman, Takashi Nakamura

    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY　54　(1)　135-140　2010

    DOI： 10.1387/ijdb.082735hc 　

    ISSN： 0214-6282

  78. Dental epithelium proliferation and differentiation regulated by ameloblastin.

    Satoshi Fukumoto, Aya Yamada, Tsutomu Iwamoto, Takashi Nakamura

    Interface Oral Health Science　33-38　2009/12

  79. Influences of interferon-gamma on cell proliferation and interleukin-6 production in Down syndrome derived fibroblasts Peer-reviewed

    Tsutomu Iwamoto, Aya Yamada, Kenji Yuasa, Emiko Fukumoto, Takashi Nakamura, Taku Fujiwara, Satoshi Fukumoto

    ARCHIVES OF ORAL BIOLOGY　54　(10)　963-969　2009/10

    DOI： 10.1016/j.archoralbio.2009.07.009 　

    ISSN： 0003-9969

  80. Influences of interferon-gamma on cell proliferation and interleukin-6 production in Down syndrome derived fibroblasts Peer-reviewed

    Tsutomu Iwamoto, Aya Yamada, Kenji Yuasa, Emiko Fukumoto, Takashi Nakamura, Taku Fujiwara, Satoshi Fukumoto

    ARCHIVES OF ORAL BIOLOGY　54　(10)　963-969　2009/10

    DOI： 10.1016/j.archoralbio.2009.07.009 　

    ISSN： 0003-9969

  81. Critical Role of Heparin Binding Domains of Ameloblastin for Dental Epithelium Cell Adhesion and Ameloblastoma Proliferation Peer-reviewed

    Akira Sonoda, Tsutomu Iwamoto, Takashi Nakamura, Emiko Fukumoto, Keigo Yoshizaki, Aya Yamada, Makiko Arakaki, Hidemitsu Harada, Kazuaki Nonaka, Seiji Nakamura, Yoshihiko Yamada, Satoshi Fukumoto

    JOURNAL OF BIOLOGICAL CHEMISTRY　284　(40)　27176-27184　2009/10

    DOI： 10.1074/jbc.M109.033464 　

    ISSN： 0021-9258

  82. エナメル芽細胞分化過程における細胞間結合の役割

    山田亜矢, 岩本勉, 鈴木宏治, 中村卓史, 原田英光, 斎藤正寛, 福本敏

    Journal of Oral Biosciences　51　(Suppl.)　139-139　2009/08

    Publisher:

    ISSN： 1349-0079

    eISSN： 1880-3865

  83. 象牙芽細胞の分化に関わる新奇ギャップ結合分子の同定

    岩本勉, 中村卓史, 吉崎恵悟, 山田亜矢, 山田吉彦, 福本敏

    Journal of Oral Biosciences　51　(Suppl.)　100-100　2009/08

    Publisher:

    ISSN： 1349-0079

    eISSN： 1880-3865

  84. 頭蓋底の軟骨結合形成及び成長における一次繊毛成分ポラリスの役割

    落合隆永, 永山元彦, 中村卓史, 長谷川博雅

    Journal of Oral Biosciences　51　(Suppl.)　97-97　2009/08

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  85. 歯原性上皮細胞におけるエピプロフィンの機能解析 Invited

    中村卓史, 岩本勉, 福本敏, 湯浅健司, 山田吉彦

    Journal of Oral Biosciences　51　(Suppl.)　73-73　2009/08

    Publisher:

    ISSN： 1349-0079

    eISSN： 1880-3865

  86. 歯と歯周組織、骨の再生 エナメル質形成の分子制御機構

    福本敏, 山田亜矢, 新垣真紀子, 福本恵美子, 岩本勉, 中村卓史

    日本骨代謝学会学術集会プログラム抄録集　27回　107-107　2009/07

    Publisher:

    ISSN： 1349-0761

  87. Roles of the Primary Cilium Component Polaris in Synchondrosis Development Peer-reviewed

    T. Ochiai, M. Nagayama, T. Nakamura, T. Morrison, D. Pilchak, N. Kondo, H. Hasegawa, B. Song, R. Serra, M. Pacifici, E. Koyama

    JOURNAL OF DENTAL RESEARCH　88　(6)　545-550　2009/06

    DOI： 10.1177/0022034509337775 　

    ISSN： 0022-0345

  88. Synergistic Roles of Amelogenin and Ameloblastin Peer-reviewed

    J. Hatakeyama, S. Fukumoto, T. Nakamura, N. Haruyama, S. Suzuki, Y. Hatakeyama, L. Shum, C. W. Gibson, Y. Yamada, A. B. Kulkarni

    JOURNAL OF DENTAL RESEARCH　88　(4)　318-322　2009/04

    DOI： 10.1177/0022034509334749 　

    ISSN： 0022-0345

  89. Identification of tooth specific genes (Proceeding of 3rd Hiroshima Conference on Education and Science in Dentistry)

    Fukumoto S, Nakamura T, Iwamoto T, Yamada A Fukumoto, E, Yamada Y

    Proceeding of 3rd Hiroshima Conference on Education and Science in Dentistry　35-37　2009

  90. 新奇細胞-細胞間蛋白質Pannexin3(Panx3)による軟骨細胞の増殖と分化の制御機構の解明

    岩本勉, 中村卓史, 福本敏, 野中和明, 山田吉彦

    Journal of Oral Biosciences　50　(Suppl.)　149-149　2008/09

    Publisher:

    ISSN： 1349-0079

    eISSN： 1880-3865

  91. 象牙芽細胞と骨芽細胞の違いを考える 象牙芽細胞特異的分子の同定と分化制御

    福本敏, 岩本勉, 山田亜矢, 中村卓史, 福本恵美子, 山田吉彦

    解剖学雑誌　83　(Suppl.)　80-80　2008/03

  92. Transcription factor epiprofin is essential for tooth morphogenesis by regulating epithelial cell fate and tooth number Peer-reviewed

    Takashi Nakamura, Susana de Vega, Satoshi Fukumoto, Lucia Jimenez, Fernando Unda, Yoshihiko Yamada

    JOURNAL OF BIOLOGICAL CHEMISTRY　283　(8)　4825-4833　2008/02

    DOI： 10.1074/jbc.M708388200 　

    ISSN： 0021-9258

  93. TM14 is a new member of the fibulin family (fibulin-7) that interacts with extracellular matrix molecules and is active for cell binding Peer-reviewed

    Susana de Vega, Tsutomu Iwamoto, Takashi Nakamura, Kentaro Hozumi, Dianalee A. McKnight, Larry W. Fisher, Satoshi Fukumoto, Yoshihiko Yamada

    JOURNAL OF BIOLOGICAL CHEMISTRY　282　(42)　30878-30888　2007/10

    DOI： 10.1074/jbc.M705847200 　

    ISSN： 0021-9258

  94. 軟骨特異的TGF-βI型受容体の欠損マウスにおける軸骨格形成異常

    鳥越清之, IshijimaMuneaki, Nakamura Takashi, HaruyamaNaoto, KulkarniAshok, KarlssonStefan, YamadaYoshihiko, 岩本幸英

    日本結合組織学会学術大会・マトリックス研究会大会合同学術集会プログラム・抄録集　39回・54回　97-97　2007/05

  95. Transcription factor ERG and joint and articular cartilage formation during mouse limb and spine skeletogenesis Peer-reviewed

    Masahiro Iwamoto, Yoshihiro Tamamura, Eiki Koyama, Toshihisa Komori, Nobuo Takeshita, Julie A. Williams, Takashi Nakamura, Motomi Enomoto-Iwamoto, Maurizio Pacifici

    DEVELOPMENTAL BIOLOGY　305　(1)　40-51　2007/05

    DOI： 10.1016/j.ydbio.2007.01.037 　

    ISSN： 0012-1606

  96. Deficiency of the G-protein alpha-subunit G(s)alpha in osteoblasts leads to differential effects on trabecular and cortical bone

    A Sakamoto, M Chen, T Nakamura, T Xie, G Karsenty, LS Weinstein

    JOURNAL OF BIOLOGICAL CHEMISTRY　280　(22)　21369-21375　2005/06

    DOI： 10.1074/jbc.M500346200 　

    ISSN： 0021-9258

  97. Deficiency of the G-protein alpha-subunit G(s)alpha in osteoblasts leads to differential effects on trabecular and cortical bone Peer-reviewed

    A Sakamoto, M Chen, T Nakamura, T Xie, G Karsenty, LS Weinstein

    JOURNAL OF BIOLOGICAL CHEMISTRY　280　(22)　21369-21375　2005/06

    DOI： 10.1074/jbc.M500346200 　

    ISSN： 0021-9258

  98. Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts Peer-reviewed

    S Fukumoto, T Kiba, B Hall, N Iehara, T Nakamura, G Longenecker, PH Krebsbach, A Nanci, AB Kulkarni, Y Yamada

    JOURNAL OF CELL BIOLOGY　167　(5)　973-983　2004/12

    DOI： 10.1083/jcb.200409077 　

    ISSN： 0021-9525

  99. kruppel様因子Epirofinは毛髪,歯,そして手足の発育に不可欠である Invited

    YamadaYoshihiko, Nakamura Takashi

    Connective Tissue　36　(2)　62-62　2004/06

  100. The Kruppel-like factor epiprofin is expressed by epithelium of developing teeth, hair follicles, and limb buds and promotes cell proliferation Peer-reviewed

    T Nakamura, F Unda, S de-Vega, A Vilaxa, S Fukumoto, KM Yamada, Y Yamada

    JOURNAL OF BIOLOGICAL CHEMISTRY　279　(1)　626-634　2004/01

    DOI： 10.1074/jbc.M307502200 　

    ISSN： 0021-9258

  101. 顎下部に発生した結核性リンパ節炎 歯科及び口腔外科領域における結核症について Peer-reviewed

    中原寛和, 北村龍二, 岸野万伸, 小原浩, 中村卓史, 鏡内肇, 棟方哲, 森野英男, 古郷幹彦, 松矢篤三

    大阪大学歯学雑誌　45　(2)　93-97　2001/03

    ISSN： 0473-4629

  102. Hedgehog proteins stimulate chondrogenic cell differentiation and cartilage formation Peer-reviewed

    M Enomoto-Iwamoto, T Nakamura, T Aikawa, Y Higuchi, T Yuasa, A Yamaguchi, T Nohno, S Noji, T Matsuya, K Kurisu, E Koyama, M Pacifici, M Iwamoto

    JOURNAL OF BONE AND MINERAL RESEARCH　15　(9)　1659-1668　2000/09

    ISSN： 0884-0431

  103. Hedgehog proteins stimulate chondrogenic cell differentiation and cartilage formation Peer-reviewed

    M Enomoto-Iwamoto, T Nakamura, T Aikawa, Y Higuchi, T Yuasa, A Yamaguchi, T Nohno, S Noji, T Matsuya, K Kurisu, E Koyama, M Pacifici, M Iwamoto

    JOURNAL OF BONE AND MINERAL RESEARCH　15　(9)　1659-1668　2000/09

    DOI： 10.1359/jbmr.2000.15.9.1659 　

    ISSN： 0884-0431

  104. 軟組織に再発した下顎骨骨肉腫の1例 Peer-reviewed

    北村龍二, 中原寛和, 中村卓史, 吉岡秀郎

    日本口腔外科学会雑誌　46　(2)　153-153　2000/02

  105. 上顎骨中心性に発生した多形性腺腫の1例 Peer-reviewed

    中村卓史, 中原寛和, 小倉孝文

    日本口腔外科学会雑誌　44　(3)　333-335　1998/03

    Publisher: Japanese Society of Oral and Maxillofacial Surgeons

    DOI： 10.5794/jjoms.44.333 　

    ISSN： 0021-5163

    More details Close

    Centrally occurring pleomorphic adenoma of the maxilla is extremely rare, although this tumor is the most common neoplasm involving both the major and minor sali-vary glands. A 32-year-old Japanese woman presented with gingival swelling of the left molar region of the maxilla. After oral and radiographic examinations, the tumor was resected with part of the maxilla under the clinical diagnosis of ameloblastoma. Microscopic examination showed that the tumor centrally occurred in the maxilla. Histopathological examination of a surgical specimen showed a benign pleomorphic adenoma. After surgery, the patient had a fairly good prognosis, with no evidence of local recurrence.

  106. Erratum: Induction of osteogenic differentiation by hedgehog proteins (Biochemical and Biophysical Research Communications (1997) 237, 2 (465-469)) Peer-reviewed

    Nakamura, T., Aikawa, T., Iwamoto-Enomoto, M., Iwamoto, M., Higuchi, Y., Pacifici, M., Kinto, N., Yamaguchi, A., Noji, S., Kurisu, K., Matsuya, T.

    Biochemical and Biophysical Research Communications　247　(3)　910-910　1998

  107. Sonic hedgehogはBMP2と相乗的に作用して軟骨形成を促進する

    中村卓史

    日本口腔外科学会雑誌　43　(13)　1027-1028　1997/12

  108. Induction of osteogenic differentiation by hedgehog proteins Peer-reviewed

    T Nakamura, T Aikawa, M IwamotoEnomoto, M Iwamoto, Y Higuchi, P Maurizio, N Kinto, A Yamaguchi, S Noji, K Kurisu, T Matsuya

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　237　(2)　465-469　1997/08

    DOI： 10.1006/bbrc.1997.7156 　

    ISSN： 0006-291X

  109. 軟骨初期分化に対するSonic hedgehogの作用とBMP-2との相乗作用

    中村卓史

    日本骨代謝学会雑誌　15　(2)　42-42　1997/06

  110. 軟骨細胞分化過程におけるCadherin family, BMP familyの発現

    中村卓史

    日本口腔科学会雑誌　45　(5)　682-682　1996/12

  111. 上顎骨中心性に発生した多形性腺腫の1例

    中村卓史

    日本口腔科学会雑誌　45　(5)　761-761　1996/12

  112. Establishment of bone morphogenetic protein 2 responsive chondrogenic cell line Peer-reviewed

    T Aikawa, K Shirasuna, M Iwamoto, K Watatani, T Nakamura, M Okura, H Yoshioka, T Matsuya

    JOURNAL OF BONE AND MINERAL RESEARCH　11　(4)　544-553　1996/04

    ISSN： 0884-0431

  113. Establishment of bone morphogenetic protein 2 responsive chondrogenic cell line Peer-reviewed

    T Aikawa, K Shirasuna, M Iwamoto, K Watatani, T Nakamura, M Okura, H Yoshioka, T Matsuya

    JOURNAL OF BONE AND MINERAL RESEARCH　11　(4)　544-553　1996/04

    DOI： 10.1002/jbmr.5650110416 　

    ISSN： 0884-0431

  114. 骨形成因子による軟骨細胞分化過程の検討

    中村卓史, 相川友直, 白砂兼光

    日本骨代謝学会雑誌　13　(2)　257-257　1995/07

    ISSN： 0910-0067

  115. ヒト唾液腺癌細胞HSGにおける骨形成因子の発現

    相沢友直, 白砂兼光, 中村卓史

    日本骨代謝学会雑誌　13　(2)　319-319　1995/07

    ISSN： 0910-0067

Show all ︎Show first 5

Misc. 52

  1. 三叉神経中脳路核における機械刺激負荷歯根膜細胞由来Wnt5aの役割

    高橋 かおり, 吉田 卓史, 中村 卓史, 若森 実

    Journal of Oral Biosciences Supplement　2023　[O3-01]　2023/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  2. 副交感神経から分泌されるアセチルコリン(ACh)が、唾液腺の発生過程で筋上皮細胞の分化誘導と機能的器官内配置を決定している(Acetylcholine(ACh), a parasympathetic neurotransmitter, orchestrates the induction and the positioning of myoepithelial cells in developing salivary glands)

    真藤 裕基, 若森 実, 中井 淳一, 中村 卓史

    Journal of Oral Biosciences Supplement　2022　250-250　2022/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  3. ビタミンDとエナメル質低石灰化症発症との関連

    中村 卓史, 若森 実, 福本 敏

    Journal of Oral Biosciences Supplement　2020　145-145　2020/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  4. 亜鉛によるMC3T3-E1細胞分化亢進作用におけるカルシウム依存性カリウムチャネルの役割

    吉田 卓史, 高橋 かおり, 中村 卓史, 若森 実

    Journal of Oral Biosciences Supplement　2018　450-450　2018/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  5. 新規basic-helix-loop-helix転写因子AmeloDは歯原性上皮細胞の遊走能を制御し歯胚形態形成に関与する

    千葉 雄太, 吉崎 恵悟, 齋藤 幹, 中村 卓史, 岩本 勉, 福本 敏, He Bing, de Vega Susana, 石島 旨章, 山田 吉彦

    Journal of Oral Biosciences Supplement　2018　285-285　2018/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  6. 歯冠・歯根長を制御する新規歯胚形態制御因子の同定と機能解明

    千葉 雄太, 齋藤 幹, 岩本 勉, 中村 卓史, 福本 敏

    小児歯科学雑誌　56　(2)　217-217　2018/04

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  7. 骨芽細胞様細胞MC3T3-E1細胞に於ける亜鉛イオンによる過分極応答

    光 夢凱, 吉田 卓史, 中村 卓史, 若森 実

    Journal of Oral Biosciences Supplement　2016　191-191　2016/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  8. エピプロフィンによる骨代謝調節

    中村 卓史, 中村 友昭, 若森 実, 福本 敏

    Journal of Oral Biosciences Supplement　2016　260-260　2016/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  9. 組織再生に関わる基礎研究基盤の現状 エピプロフィンは歯原性上皮細胞のエナメル芽細胞系への誘導と分化を制御する多機能制御因子である

    山田 吉彦, 酒井 陽, 千葉 雄太, Mahboubi Darius, 池内 友子, 石河 真幸, 中村 卓史, 福本 敏

    Journal of Oral Biosciences Supplement　2016　57-58　2016/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  10. 【歯の細胞生物学】 エナメル芽細胞の分化制御機構

    中村 卓史, 成瀬 正啓, 齋藤 幹, 福本 敏

    腎と骨代謝　29　(1)　15-24　2016/01

    Publisher: (株)日本メディカルセンター

    DOI： 10.19020/J02201.2016149814 　

    ISSN： 0914-5265

    eISSN： 2433-2496

  11. エナメル形成と石灰化におけるビタミンDの役割

    中村 卓史

    松本歯学　41　(2)　185-186　2015/12

    Publisher: 松本歯科大学学会

    ISSN： 0385-1613

    eISSN： 2188-7233

  12. 発生から考えた歯の再生

    福本敏, 新垣真紀子, 山田亜矢, 齋藤幹, 中村卓史

    日本抗加齢医学会雑誌　11　(2)　188-193　2015/05

    Publisher: (株)メディカルレビュー社

    ISSN： 1880-1579

  13. 歯の萠出時期と嚢胞形成に関わるインテグリンbeta1の分子機構について

    齋藤 幹, 山田 亜矢, 新垣 真紀子, 二木 正晴, 中村 卓史, 福本 敏

    小児歯科学雑誌　53　(2)　205-205　2015/04

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  14. エピプロフィンによる上皮細胞増殖制御機構(Molecular mechanism of Epiprofin in epithelial cell proliferation)

    中村 卓史, 福本 敏

    Journal of Oral Biosciences Supplement　2014　123-123　2014/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  15. アメロブラスチン受容体グリピカン1の歯胚発生における発現とその機能

    浦川 麻衣子, 二木 正晴, 只木 麻夕, 斎藤 幹, 中村 卓史, 福本 敏

    小児歯科学雑誌　52　(2)　332-332　2014/04

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  16. 歯髄幹細胞分化における細胞内シグナル制御機構の解明

    菅原 優, 岩本 勉, 小野 真理子, 新垣 真紀子, 中村 卓史, 福本 敏

    小児歯科学雑誌　52　(2)　351-351　2014/04

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  17. 新規転写因子エピプロフィンによる副甲状腺ホルモン発現制御

    中村卓史, 福本敏, 中村はな, 千葉雄太, 山田吉彦, 岩本容泰

    月刊内分泌・糖尿病・代謝内科　38　(2)　165-171　2014/02/28

    Publisher: (有)科学評論社

    ISSN： 1884-2917

  18. The influence of Sox21 as a novel ameloblast marker on tooth germ differentiation

    SAITO Kan, YAMADA Aya, NAKAMURA Takashi, FUKUMOTO Satoshi

    51　(2)　158-158　2013/04/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  19. Analysis of Connexin43-FGF10 signaling on salivary gland development

    FUKUMOTO Satoshi, FUTAGI Masaharu, IWAMOTO Tsutomu, NAKAMURA Takashi, YAMADA Aya, SAITO Kan

    51　(2)　156-156　2013/04/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  20. Regulation of dental epithelial cell proliferation and differentiation by laminin

    OIKAWA Satoko, FUTAGI Masaharu, YAMADA Aya, ARAKAKI Makiko, MIYAMOTO Ryoko, SAITO Kan, NAKAMURA Takashi, FUKUMOTO Satoshi

    51　(2)　274-274　2013/04/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  21. 歯胚形成におけるビタミンDの役割

    池内 友子, 千葉 雄太, 成瀬 正啓, 新垣 真紀子, 中村 卓史, 福本 敏

    小児歯科学雑誌　51　(2)　270-270　2013/04/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  22. 骨・カルシウム代謝Update 副甲状腺における新規転写因子エピプロフィンの役割

    中村 卓史

    日本内分泌学会雑誌　89　(1)　171-171　2013/04

    Publisher: (一社)日本内分泌学会

    ISSN： 0029-0661

    eISSN： 2186-506X

  23. 乳歯歯髄を用いた再生医療における課題について

    二木 かおり, 山田 亜矢, 新垣 真紀子, 岩本 勉, 中村 卓史, 福本 敏

    小児歯科学雑誌　51　(1)　78-79　2013/03

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  24. 歯科再生利用実現化を目指した新しい分子機能予測法開発

    及川 知子, 只木 麻友, 成瀬 正啓, 新垣 真紀子, 岩本 勉, 山田 亜矢, 中村 卓史, 福本 敏

    小児歯科学雑誌　51　(1)　111-112　2013/03

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  25. Novel Compounds Mimic Hedgehog Activity and Promote Osteoblast Differentiation in C3H10T1/2 Cells and Osteoblastic Cells from Runx2-Deficient Mice

    Takashi Nakamura, Masahiro Naruse, Tomoko Ikeuchi, Toshihisa Komori, Aya Yamada, Masahiro Iwamoto, Satoshi Fukumoto

    JOURNAL OF BONE AND MINERAL RESEARCH　28　2013/02

    ISSN： 0884-0431

    eISSN： 1523-4681

  26. エピプロフィン遺伝子の同定とその機能解析

    中村 卓史

    松本歯学　38　(2)　201-202　2012/12

    Publisher: 松本歯科大学学会

    ISSN： 0385-1613

    eISSN： 2188-7233

  27. エピプロフィン欠損マウスモデルの解析

    中村 卓史

    Journal of Oral Biosciences Supplement　2012　77-77　2012/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  28. 歯の発生におけるスフィンゴ糖脂質の役割

    千葉 雄太, 中村 卓史, 成瀬 正啓, 池内 友子, 新垣 真紀子, 福本 敏

    Journal of Oral Biosciences Supplement　2012　108-108　2012/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  29. フィラミン-Aによる歯根形成メカニズムの解明

    宮本 綾子, 山田 亜矢, 岩本 勉, 中村 卓史, 福本 敏

    小児歯科学雑誌　50　(2)　168-168　2012/04/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  30. エナメル芽細胞分化過程におけるテトラスパニンCD9の発現とその役割

    岩本 勉, 小野 真理子, 二木 正晴, 菅原 優, 山田 亜矢, 中村 卓史, 福本 敏

    小児歯科学雑誌　50　(2)　173-173　2012/04/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  31. Melnick-Needles 症候群における歯の形成異常メカニズム

    宮本 綾子, 山田 亜矢, 岩本 勉, 中村 卓史, 福本 敏

    小児歯科学雑誌　49　(4)　331-331　2011/10/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  32. 人工歯髄幹細胞における骨芽細胞分化誘導の評価

    山田 亜矢, 中村 卓史, 二木 正晴, 新垣 真紀子, 菅原 優, 岩本 勉, 福本 敏

    小児歯科学雑誌　49　(4)　334-334　2011/10/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  33. 新規小分子化合物を用いた歯原性上皮細胞の増殖と分化制御

    成瀬 正啓, 中村 卓史, 新垣 真紀子, 池内 友子, 山田 亜矢, 岩本 勉, 福本 敏

    小児歯科学雑誌　49　(4)　335-335　2011/10

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  34. 新規小分子化合物による骨芽細胞分化誘導

    中村 卓史, 成瀬 正啓, 池内 友子, 新垣 真紀子, 福本 敏

    Journal of Oral Biosciences　53　(Suppl.)　116-116　2011/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  35. 小児科医のための子どもの歯科 歯の再生

    山田 亜矢, Tsutomu Iwamoto, 中村 卓史, 福本 敏

    Japanese Journal of Pediatric Medicine　Vol.43　(No.8)　1396-1399　2011/08

    Publisher: (株)東京医学社

    ISSN： 0385-6305

  36. パネキシン3は細胞-基質間相互作用により象牙芽細胞の増殖と分化を調和させる(Pannexin 3 coordinates odontoblasts proliferation and differentiation by cell-matrix interaction)

    二木 正晴, 岩本 勉, 新垣 真紀子, 山田 亜矢, 小野 真理子, 中村 卓史, 福本 敏

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　83回・33回　4P-0702　2010/12

    Publisher: (公社)日本生化学会

  37. 歯および皮膚の発生におけるエピプロフィンの役割(Roles of Epiprofin in tooth and skin development)

    中村 卓史, 吉冨 康夫, 岩本 勉, 山田 吉彦, 福本 敏

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　83回・33回　1P-0951　2010/12

    Publisher: (公社)日本生化学会

  38. 歯発生における血小板由来増殖因子の機能的役割(Functional roles of platelet-derived growth factors in tooth development)

    菅原 優, 岩本 勉, 二木 正晴, 山田 亜矢, 新垣 真紀子, 小野 真理子, 中村 卓史, 福本 敏

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　83回・33回　1P-0953　2010/12

    Publisher: (公社)日本生化学会

  39. 歯の発生過程におけるマイクロRNAの発現変化と役割

    田中 康二朗, 中村 卓史, 山田 亜矢, 岩本 勉, 新垣 真紀子, 前田 奈名子, 福本 敏

    小児歯科学雑誌　48　(5)　570-570　2010/11/10

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  40. 器官培養系を用いた粘液嚢胞モデルの確立

    福本 敏, 鈴木 宏治, 岩本 勉, 山田 亜矢, 二木 正晴, 中村 卓史

    小児歯科学雑誌　48　(5)　561-561　2010/11/10

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  41. 歯冠の近遠心径および頬舌径の分子制御機構

    岩本 勉, 山田 亜矢, 中村 卓史, 自見 英治郎, 福本 敏

    小児歯科学雑誌　48　(5)　571-571　2010/11/10

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  42. Regulation of ameloblastin expression via gap junctional communication

    Aya Yamada, Tsutomu Iwamoto, Takashi Nakamura, Satoshi Fukumoto

    FASEB JOURNAL　24　2010/04

    ISSN： 0892-6638

  43. Expression of filamin-A during tooth development

    Ryoko Miyamoto, Aya Yamada, Tsutomu Iwamoto, Takashi Nakamura, Satoshi Fukumoto

    FASEB JOURNAL　24　2010/04

    ISSN： 0892-6638

  44. 顎顔面発生研究の新規展開 若手研究者の発想とねらい 上皮器官形成における転写因子エピプロフィンの役割

    中村 卓史, 山田 吉彦, 福本 敏

    解剖学雑誌　85　(Suppl.)　96-96　2010/03

    Publisher: (一社)日本解剖学会

    ISSN： 0022-7722

  45. 下顎第二乳臼歯の埋伏症例における不正咬合について

    新垣 真紀子, 岩本 勉, 山田 亜矢, 鈴木 宏治, 中村 卓史, 福本 敏

    小児歯科学雑誌　48　(1)　114-114　2010/03

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  46. 頭蓋底の軟骨結合形成及び成長における一次繊毛成分ポラリスの役割(Roles of Primary Cilia Component Polaris in Cranial Base Synchondrosis Development and Growth)

    落合 隆永, 永山 元彦, 中村 卓史, 長谷川 博雅

    Journal of Oral Biosciences　51　(Suppl.)　81-81　2009/08

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  47. 肢芽未分化間葉細胞高密度培養に対するSonic Hedgehogの影響

    畠山 雄次, 畠山 純子, Nakamura Takashi, 田中 康二朗, 高橋 一郎, 笹野 泰之, 真柳 秀昭

    Journal of Oral Biosciences　47　(Suppl.)　169-169　2005/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  48. Abnormal axial skeleton in TGF-beta type I receptor-deficient mice.

    K Torigoe, M Ishijima, T Nakamura, N Haruyama, AB Kulkarni, S Karlsson, Y Yamada

    JOURNAL OF BONE AND MINERAL RESEARCH　20　(9)　S49-S49　2005/09

    ISSN： 0884-0431

  49. Epiprofin, a Kruppel-like factor, is essential for hair, tooth, and limb development

    YAMADA Yoshihiko, NAKAMURA Takashi

    Connective tissue　36　(2)　62-62　2004/06/25

    Publisher: The Japanese Society for Connective Tissue

    ISSN： 0916-572X

    More details Close

    We identified a cDNA clone for a nuclear factor that we named epiprofin by differential hybridization using DNA microarrays from an embryonic day 1 9.5 mouse molar cDNA library. Full-length cloning and sequencing revealed that epiprofin encodes a member of the Kruppel-Like Factor (KLF) family containing three characteristic C2H2-type zinc-finger motifs. Except for its 5' terminal sequence, the epiprofin mRNA sequence is almost identical to the predicted sequence of KLF14/SP6, which was previously identified in EST databases and GenBank through a sequence homology search for an Sp1 zinc-finger DNA binding domain. In situ hybridization revealed that epiprofin mRNA is expressed by proliferating hair follicle matrix epithelium and also dental epithelium. In addition, whole-mount in situ hybridization showed transient expression of epiprofin mRNA in cells of the apical ectodermal ridge (AER) in developing limb and the posterior neuropore. Transfection of an epiprofin expression vector showed that epiprofin is localized in the nucleus and promotes cell proliferation, suggesting that epiprofin is a highly cell- and tissue-specific nuclear protein expressed primarily by proliferating epithelial cells of hair follicle, tooth, and limb. Homozygous epiprofin knockout mice survive but are hairless, toothless, and have fusion and/or missing digits, indicating the essential role of epiprofin in the development of these tissues.

  50. Erratum: Induction of osteogenic differentiation by hedgehog proteins (Biochemical and Biophysical Research Communications (1997) 237, 2 (465-469))

    T. Nakamura, T. Aikawa, M. Iwamoto-Enomoto, M. Iwamoto, Y. Higuchi, M. Pacifici, N. Kinto, A. Yamaguchi, S. Noji, K. Kurisu, T. Matsuya

    Biochemical and Biophysical Research Communications　247　910　1998/06/29

    ISSN： 0006-291X

  51. Induction of osteogenic differentiation by hedgehog proteins (vol 237, pg 465, 1997)

    T Nakamura, T Aikawa, M Iwamoto-Enomoto, M Iwamoto, Y Higuchi, P Maurizio, N Kinto, A Yamaguchi, S Noji, K Kurisu, T Matsuya

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　247　(3)　910-910　1998/06

    ISSN： 0006-291X

  52. The Incidence of Cleft Lip and/or Palate in the Suburbs of Osaka Prefecture in Japan.

    足立忠文, 古郷幹彦, 岡田元, 田中晋, 茶野珠里, 富永仰, 中島昌宗, 中村卓史, 松矢篤三

    大阪大学歯学雑誌　41　(1)　240-247　1996/06

    ISSN： 0473-4629

Show all ︎Show first 5

Books and Other Publications 3

  1. 新規転写因子エピプロフィンによる副甲状腺ホルモン発現制御

    Takashi Nakamura, Satoshi Fukumoto, Hannah M Nakamura, Yuta Chiba, Yoshihiko Yamada, Masahiro Iwamoto

    2014/02/01

  2. 小児歯科で取り組む再生医療

    福本敏, 山田亜矢, 岩本勉, 中村卓史

    日本小児医事出版社　2010/11

  3. 歯の再生-歯の発生生物学から歯の再生研究まで

    中村 卓史, 上田実

    真興交易医書出版部　2008/09/01

Presentations 42

  1. 100年つかえる丈夫な永久歯には、 おなかの中にいるときの栄養が関係しているの？ Invited

    中村卓史

    至学館大学公開講座 2024年度　2024/10/03

  2. Elucidation of neuronal-epithelial communication in murine submandibular development Invited

    Takashi Nakamura

    Korean Division of theInternational Association for Dental Research　2022/09/22

  3. Roles of Epiprofin in dental epithelial cell differentiation International-presentation

    NAKAMURA Takashi

    IOHS 2019　2019/01/11

  4. 副甲状腺ホルモンの転写制御に関わるエピプロフィンの役割 Invited

    中村 卓史

    第36回内分泌代謝学サマーセミナー　2018/08/02

  5. The effect of three-dimensional culture on the ameloblastic differentiation of dental epithelial cells

    Takahisa Anada, Mayu Tadaki, Mayu Tadaki, Yukari Shiwaku, Yukari Shiwaku, Takashi Nakamura, Masanori Nakamura, Masaru Kojima, Tatsuo Arai, Satoshi Fukumoto, Osamu Suzuki

    2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016　2017/01/18

    More details Close

    © 2016 IEEE. We have developed three-dimensional (3D) cell culture devices. The present study investigated that the effect of 3D culture of rat incisor-derived dental epithelial cells on their differentiation into ameloblast-like cells.

  6. 組織再生に関わる基礎研究基盤の現状 エピプロフィンは歯原性上皮細胞のエナメル芽細胞系への誘導と分化を制御する多機能制御因子である

    山田 吉彦, 酒井 陽, 千葉 雄太, Mahboubi Darius, 池内 友子, 石河 真幸, 中村 卓史, 福本 敏

    Journal of Oral Biosciences Supplement　2016/09

  7. 骨芽細胞様細胞MC3T3-E1細胞に於ける亜鉛イオンによる過分極応答

    光 夢凱, 吉田 卓史, 中村 卓史, 若森 実

    Journal of Oral Biosciences Supplement　2016/09

  8. エピプロフィンによる骨代謝調節

    中村 卓史, 中村 友昭, 若森 実, 福本 敏

    Journal of Oral Biosciences Supplement　2016/09

  9. Transcriptional regulation of Epiprofin International-presentation

    NAKAMURA Takashi

    TMD 2016　2016/06/13

  10. エナメル形成と石灰化におけるビタミンDの役割

    中村 卓史

    松本歯学　2015/12

  11. 歯の萠出時期と嚢胞形成に関わるインテグリンbeta1の分子機構について

    齋藤 幹, 山田 亜矢, 新垣 真紀子, 二木 正晴, 中村 卓史, 福本 敏

    小児歯科学雑誌　2015/04

  12. Wnt5a attenuates Wnt3a-induced alkaline phosphatase expression in dental follicle cells

    Yukihiko Sakisaka, Masahiro Tsuchiya, Masahiro Tsuchiya, Takashi Nakamura, Takashi Nakamura, Masato Tamura, Hidetoshi Shimauchi, Eiji Nemoto

    Experimental Cell Research　2015/01/01

    More details Close

    © 2015 Elsevier Inc. Wnt signaling regulates multiple cellular events such as cell proliferation, differentiation, and apoptosis through β-catenin-dependent canonical and β-catenin-independent noncanonical pathways. Canonical Wnt/β-catenin signaling can promote the differentiation of dental follicle cells, putative progenitor cells for cementoblasts, osteoblasts, and periodontal ligament cells, toward a cementoblast/osteoblast phenotype during root formation, but little is known about the biological significance of noncanonical Wnt signaling in this process. We identified the expression of Wnt5a, a representative noncanonical Wnt ligand, in tooth root lining cells (i.e. precementoblasts/cementoblasts) and dental follicle cells during mouse tooth root development, as assessed by immunohistochemistry. Silencing expression of the Wnt5a gene in a dental follicle cell line resulted in enhancement of the Wnt3a (a representative canonical Wnt ligand)-mediated increase in alkaline phosphatase (ALP) expression. Conversely, treatment with recombinant Wnt5a inhibited the increase in ALP expression, suggesting that Wnt5a signaling functions as a negative regulator of canonical Wnt-mediated ALP expression of dental follicle cells. Wnt5a did not affect the nuclear translocation of β-catenin as well as β-catenin-mediated transcriptional activation of T-cell factor (Tcf) triggered by Wnt3a, suggesting that Wnt5a inhibits the downstream part of the β-catenin-Tcf pathway. These findings suggest the existence of a feedback mechanism between canonical and noncanonical Wnt signaling during the differentiation of dental follicle cells.

  13. Regulation of bone homeostasis by the transcription factor Epiprofin International-presentation

    Takashi Nakamura, Satoshi Fukumoto, Takahiro Okabe, Takanaga Ochiai, Eiki Koyama, Motomi Enomoto-Iwamoto, Maurizio Pacifici, Masahiro Iwamoto, Yoshihiko Yamada

    ASBMR 2011　2011/09/16

  14. Nerve growth factor (NGF) promotes dental epithelial cell proliferation International-presentation

    Mariko Ono, Tsutomu Iwamoto, Yu Sugawara, Aya Yamada, Takashi Nakamura, Satoshi Fukumoto

    ASBMR 2011　2011/09/16

  15. Pannexin 3 plays a critical role for Odontoblast Differentiation International-presentation

    Tsutomu Iwamoto, Yu Sugawara, Mariko Ono, Aya Yamada, Takashi Nakamura, Satoshi Fukumoto

    ASBMR 2011　2011/09/16

  16. Platelet-derived growth factors (PDGFs) functions in tooth development International-presentation

    Yu Sugawara, Tsutomu Iwamoto, Masaharu Futagi, Mariko Ono, Aya Yamada, Takashi Nakamura, Satoshi Fukumoto

    ASBMR 2011　2011/09/16

  17. Roles of Epiprofin in hair follicle development International-presentation

    Takashi Nakamura, Yasuo Yoshitomi, Makiko Arakaki, Yoshihiko Yamada, Satoshi Fukumoto

    44th Annual Meeting for the Japanese Society of Developmental Biologists　2011/05/18

  18. Induction of ameloblasts from induced pluripotent stem cells International-presentation

    Makiko Arakaki, Takashi Nakamura, Tsutomu Iwamoto, Aya Yamada, Satoshi Fukumoto

    44th Annual Meeting for the Japanese Society of Developmental Biologists.　2011/05/18

  19. Multiple roles of Epiprofin in ectodermal organs International-presentation

    TAKASHI NAKAMURA, Yasuo Yoshitomi, Yoshihiko Yamada, Tsutomu Iwamoto, Aya Yamada, Satoshi Fukumoto

    43rd Annual Meeting for the Japanese Society of Developmental Biologists　2011/02/14

  20. Roles of Epiprofin in tooth and skin development

    Takashi Nakamura, Yasuo Yoshitomi, Tsutomu Iwamoto, Yoshihiko Yamada, Satoshi Fukumoto

    第33回日本分子生物学会年会　2010/12/07

  21. Pannexin 3 coordinates odontoblasts proliferation and differentiation by cell-matrix interaction

    Masaharu Futagi, Tsutomu Iwamoto, Makiko Arakaki, Aya Yamada, Mariko Ono, Takashi Nakamura, Satoshi Fukumoto

    第33回日本分子生物学会年会　2010/12/07

  22. Functional roles of platelet-derived growth factors in tooth development

    Yu Sugawara, Tsutomu Iwamoto, Masaharu Futagi, Aya Yamada, Makiko Arakaki, Mariko Ono, Takashi Nakamura, Satoshi Fukumoto

    第33回日本分子生物学会年会　2010/12/07

  23. 器官培養系を用いた粘液嚢胞モデルの確立

    福本 敏, 鈴木宏治, 岩本 勉, 山田亜矢, 二木正晴, 中村卓史

    平成22年度一般社団法人日本小児歯科学会秋季大会　2010/12/02

  24. 歯の発生過程におけるマイクロRNAの発現変化と役割

    田中康二朗, 中村卓史, 山田亜矢, 岩本勉, 新垣真紀子, 前田奈名子, 福本敏

    平成22年度一般社団法人日本小児歯科学会秋季大会　2010/12/02

  25. 歯冠の近遠心径および頬舌径の分子制御機構

    岩本 勉, 山田亜矢, 中村卓史, 自見英治郎, 福本 敏

    平成22年度一般社団法人日本小児歯科学会秋季大会　2010/12/02

  26. 合成ビタミンD誘導体で前処理したヒト単球系細胞の自然免疫応答、特にNOD2応答増強とそのメカニズム

    池内友子, 中村卓史, 福本敏, 高田春比古

    第16回日本エンドトキシン・自然免疫研究会　2010/11/12

  27. インテグリンーフィラミンA複合体による歯の形態形成の分子制御

    宮本綾子, 山田亜矢, 中村 卓史, 岩本 勉, 小野真理子, 福本 敏

    第52回歯科基礎医学会学術大会・総会　2010/09/20

  28. 歯原性上皮の分化の分子制御メカニズム

    福本敏, 中村卓史, 山田亜矢, 新垣真紀子, 岩本勉

    第52回歯科基礎医学会学術大会・総会　2010/09/20

  29. 象牙芽細胞分化におけるギャップ結合分子の発現および機能解析

    岩本勉, 中村卓史, 吉崎恵吾, 山田亜矢, 福本敏

    第52回歯科基礎医学会学術大会・総会　2010/09/20

  30. 神経栄養因子による歯原性上皮細胞の増殖、分化制御機構の解明

    小野真理子, 岩本勉, 吉崎恵吾, 中村卓史, 山田亜矢, 宮本綾子, 福本敏

    第52回歯科基礎医学会学術大会・総会　2010/09/20

  31. GSK 3beta inhibitor promotes reprogramming of detal pulp cell International-presentation

    Yamada, A, Nakamura, T, Iwamoto, T, Fukumoto, S

    IADR General Session　2010/07/14

  32. Induction of Ameloblasts from induced pluriopotent stem cells International-presentation

    Arakaki, M, Nakamura, T, Yamada, A, Suzuki, H, Iwamoto, T, Fukumoto, S

    IADR General Session　2010/07/14

  33. エピプロフィンによる歯原性上皮細胞の増殖と分化の制御機構

    中村卓史, 岩本勉, 山田亜矢, 福本敏

    第48回一般社団法人日本小児歯科学会大会　2010/05/19

  34. 唾液腺分岐におけるギャップ結合の役割解明と再生への応用

    鈴木宏治, 山田亜矢, 岩本勉, 中村卓史, 福本敏

    第48回一般社団法人日本小児歯科学会大会　2010/05/19

  35. Essential roles of zinc-figer factor epiprofin in tooth development International-presentation

    Takashi Nakamura, Kenji Yuasa, Lucia Jimenez, Fernando Unda, Eiki Koyama, Satoshi Fukumoto, Yoshihiko Yamada

    ASBMB ANNUAL MEETING　2010/04/24

  36. Regulation of ameloblastin expression via gap junctional communication International-presentation

    Aya Yamada, Tsutomu Iwamoto, Takashi Nakamura, Satoshi Fukumoto

    ASBMB ANNUAL MEETING　2010/04/24

  37. Expression of filamin-A during tooth development International-presentation

    Ryoko Miyamoto, Aya Yamada, Tsutomu Iwamoto, Takashi Nakamura, Satoshi Fukumoto

    ASBMB ANNUAL MEETING　2010/04/24

  38. Pannexin 3 regulates intracellular ATP/cAMP levels and promotes chondrocyte differentiation International-presentation

    Tsutomu Iwamoto, Takashi Nakamura, Andrew Doyle, Masaki Ishikawa, Susana de Vega, Satoshi Fukumoto, Yoshihiko Yamada

    ASBMB ANNUAL MEETING　2010/04/24

  39. Regulation of PTHrP gene in articular chondrocytes

    Okabe, T, Kondo, N, Williams, J, Nakamura, T, Wakitani, S, Pacifici, M, Iwamoto, I

    第23回日本軟骨代謝学会　2010/04/02

  40. 顎顔面発生研究の新規展開 若手研究者の発想とねらい 上皮器官形成における転写因子エピプロフィンの役割

    中村卓史, 山田吉彦, 福本敏

    第115回日本解剖学会総会・全国学術集会　2010/03/28

  41. エナメル質形成の分子制御機構

    福本敏, 山田亜矢, 新垣真紀子, 福本恵美子, 岩本勉, 中村卓史

    第27回日本骨代謝学会　2009/07/23

  42. Epiprofin, a transcription factor expressed in ectoderm organs, is essential for tooth morphogenesis International-presentation

    Takashi Nakamura, Yoshihiko Yamada

    84th General Session & Exhibition of the IADR　2006/06/28

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Research Projects 16

  1. オートファジーによる歯の幹細胞老化制御メカニズムの解明

    依田 浩子, 大島 勇人, 中村 卓史

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 新潟大学

    2024/04/01 - 2027/03/31

  2. Molecular mechanisms of Epiprofin in dental epithelial cells.

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2024/04/01 - 2027/03/31

  3. 唾液腺発生における神経-器官相互作用の分子機構の解明と機能再生技術への応用

    中村 卓史

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 国際共同研究加速基金(国際共同研究強化(B))

    Category: 国際共同研究加速基金(国際共同研究強化(B))

    Institution: 東北大学

    2021/10/07 - 2025/03/31

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    歯、毛包、腺組織、腎臓、肺、消化管組織に存在する上皮組織は、共通性のある上皮間葉相互作用により発生し、転写因子エピプロフィン（Epfn）は上皮細胞の増殖、枝分かれ、運命決定、組織幹細胞維持、細胞死を制御し上皮組織の発生形態形成、また皮膚、毛包などでは組織恒常性維持のため幹細胞維持とTransient-amplifying (TA)細胞の増殖と運命決定に重要である。完成した器官が、支配神経制御下で生体機能を発揮するためには、器官形成時に神経終末が効果器となる細胞（唾液腺では主に筋上皮細胞）に隣接して分化誘導され組織内に配置される必要がある。すなわち自律神経制御を受ける唾液腺は、器官完成後分泌を促進させる指令を出す副交感神経とその指令を受け唾液を分泌させるために腺房・導管を収縮させる筋上皮細胞が近接されて器官設計されることが必要である。これらの器官形成には、歯、毛包、腺組織、腎臓、肺、消化管組織形成期に展開される共通性のある上皮間葉相互作用が重要である。転写因子エピプロフィン（Epfn）は上皮細胞の増殖、枝分かれ、運命決定、組織幹細胞維持、細胞死を制御しているが、神経―上皮組織間の相互作用にも関与している事が、強く示唆される結果を得た。本研究の目的は、唾液腺器官培養系を用いて神経伝達物質とEpfnがどのように共役し、唾液腺上皮細胞の増殖と運命決定と組織内の細胞配置を制御しているのかを解明することであり、海外共同研究では、放射線照射モデルを利用し、障害唾液腺の再生を発生過程での神経伝達物質とEpfnの作用を応用することによる効果的で機能的な再生器官誘導法樹立への道筋をたてることである。

  4. エピプロフィンによる上皮間葉転換制御機構を応用したがん治療と器官原器複製術の開発

    中村 卓史, 中村 はな, 若森 実

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2021/04/01 - 2024/03/31

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    本研究は、器官形成期に一部の細胞集団が一塊となり細胞遊走能を獲得し間葉組織へと陥入し器官原器を形成する機構の解明のみならず、1つの歯堤から複数の歯胚や後続永久歯胚の形成機構、毛包器官のself-renewing機構の解明の一助になる。また、がん細胞がEMT状態へ移行し、細胞遊走能を活性化しそして転移する新たなメカニズムの解明にもつながるのではないかと考えている。また、本研究は転移しないがん細胞への形質転換治療という新たな治療法や器官原器数を増やす器官再生技術の開発を目指し、Epfnの発現制御に関わる小分子化合物をリード化合物として誘導体を合成し、新規物質の創製などへの研究の発展性もある。 上皮細胞が移動能を有する間葉細胞に転換する上皮間葉転換(Epithelial to mesenchymal transition; EMT)は、胚の初期発生や器官形成に重要である。この現象は発生時のみならず、創傷治癒、腫瘍細胞の転移などでも起こり、EMTに関連する蛋白は、がんのバイオマーカーにも使われている。転写因子エピプロフィン(Epfn)発現が欠失した上皮および歯原性上皮細胞は、細胞移動能が亢進し間葉への上皮陥入が継続する。つまりEpfnはEMTを負に制御していることが示唆される。以上のことから本研究では細胞遊走・移動におけるEpfnの役割解明を目的とする。さらにEpfn発現を人為的に操作し上皮細胞の遊走を制御することにより、新たながん治療法や器官原器複製による再生技術の開発基盤となる知見獲得を目指す 。

  5. Study on neural mechanism of swallowing by imaging

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2020/04/01 - 2023/03/31

  6. Elucidation of voltage-gated calcium channel function in nonexcitatory cells based on clinical genomic studies

    Wakamori Minoru

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2020/04/01 - 2023/03/31

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    The mutation of p.A36V in CACNA1C may be an ultra-rare single-nucleotide variant in Japan individuals. CACNA1C with control or this mutation was transfected into BHK cells that constantly expressed the auxiliary subunits (α2/δ and β2 subunits) of voltage-gated Ca2+ channels. After 3 days, the whole-cell mode of the patch-clamp technique was applied to BHK cells, and the membrane current was recorded under the voltage-clamp condition. This mutation reduced Ca2+-dependent inactivation and resulted in excessive Ca2+ influx via mutation channels. These results suggest that this novel single-nucleotide variant in CACNA1C may contribute to the risk of schizophrenia by affecting Ca2+ homeostasis.

  7. Controlling of organ morphology by anchor proteins in regenerative organogenesis

    Nakamura Takashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2018/06/29 - 2020/03/31

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    In the present study, we constructed a single-cell RNA sequence library from developing mouse tooth germ. We performed the rebuild the data on computer-based organ mapping, and performed individual gene expression profiling of dental epithelial cells. As a result, we succeeded in categorizing a variety of dental epithelial cells, and focus on the expression of gap junction protein connexin 43 (Cx43). We found that microRNA-1 (miR-1) is involved in Cx43 protein expression, and miR-1 inhibits Cx43 translation during tooth development and negatively regulates the proliferation of dental epithelial cells. Furthermore, the negative regulation in cell proliferation was associated to ATP release by the formation of hemi-channel Cx43 in differentiating dental epithelial cells with the modification of the cellular localization of Cx43.

  8. Establishment of Control Theory for Cementum Formation based on Wnt and Dentin Matrix Protein

    NEMOTO Eiji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2016/04/01 - 2020/03/31

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    The purpose of this study is to find a substance that effectively acts on the cementum formation, which plays an important role in the firm adhesion of the root and gingival tissurs. In this study, it was verified that the dentin sialoprotein contained in the dentin which constitutes the tooth root has the action which induces the cementum formation. Using genetic engineering techniques, we synthesized recombinant dentin sialoprotein and found that it regulated the differentiation of cementoblasts. These results lead to the development of periodontal tissue regeneration theory.

  9. Epiprofin regulation in cell proliferation and differentiation in tooth development

    Nakamura Takashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    We previously identified Epiprofin(Epfn) as a novel member of Sp transcription factor family that is expressed in certain ectodermal organs, such as teeth, hair follicles, nails and skin. During tooth development, Epfn regulates cell proliferation as well as cell migration at the early stages and cell differentiation in the later mature stage by controlling the phosphorylation of Epfn. We also identified a small molecule that strongly induced the expression of Epfn in dental epithelial cells. We are applying the Epfn -inducible small molecule in enamel biosynthesis of regenerative dentistry.

  10. Influence of chromosome 13 abnormality on hard tissue

    SAITO KAN

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    The osteoblasts were collected from Sox21KO and WT mouse, and the expression of the bone metabolism marker was investigated by RT-PCR. The decrease of expression level was not observed in the Sox21KO mouse. Next, the effect on osteoclasts was examined. The osteoclasts differentiation potency was compared by TRAP staining from the macrophage of spleen origin, and the difference was not found in this either. It was considered indirect effect through the other cells from these results. A blood was tested since Sox21 is expressed in the blood. No changes major in the item about the bone metabolism were identified. The osteoclast differentiation was induced from the bone marrow cell collected from the mouse femur because Sox21 was expressed in the bone marrow too. The number of osteoclasts in the Sox21KO Mouse increased compared with WT mouse.

  11. Vitamin D regulates dental epithelial cell differentiation and enamel formation

    Nakamura Takashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    The aim of this research is to elucidate the mechanism of vitamin D during enamel formation as well as enamel mineralization. 1,25(OH)2D3 promoted the expression of a series of enamel matrix proteins such as Ameloblastin, enamelin, and amelogenin in dental epithelial cells. Thus, 1,25(OH)2D3 promotes dental epithelial cell differentiation into ameloblast. In addition, long term culture of dental epithelial cells in the presence of 10-7M 1,25(OH)2D3 with mineralization media induced the mineralization of culture cells as well as secreted matrices. Attrition of the tip of incisors and molars are observed in rats which feed vitamin D-deficient diet for 12 weeks. Furthermore, incisors of vitamin D-deficient rats showed chalky white enamel suggesting enamel hypomineralization. These results suggest that 1,25(OH)2D3 is an essential vitamin for normal enamel formation.

  12. Function and regulation of gap junction on oral epithelium organogenesis

    Yamada Aya, NAKAMURA TAKASHI, SAITO MASAHIRO, SAKAI TAKAYOSHI, IWAMOTO TSUTOMU

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2012/04/01 - 2016/03/31

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    Connexin 43 is one of the gap junction protein. To understand the role of connexin 43 (Cx43) in salivary gland development, first we examined the expression of Cx43 by immunostaining using their specific antibody. Cx43 expressed terminal bud epithelum of salivary gland, but not in mesenchymal tisuue. In Cx43 knockout mice, branching morphogenesis of submandibular gland was dramatically inhibited. In presence of inhibitory peptide for Cx43 function, branching morphogenesis of salivary gland organ culture was also inhibited as similar to Cx43 knockout mice. From our results, Cx43 is necessary for salivary gland development and terminal bud branching. Further, gap junctional communication is important for the determination of cell fate. These information is useful for understanding the molecular mechanism of salivary gland development and regeneration.

  13. Elucidate the regulational mechanism of tooth bud formation

    NAKAMURA TAKASHI, SAKAI Takayoshi, SAITO Masahiro, FUKUMOTO Satoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    The final goal of this study is to elucidate the molecular mechanism of the regulation in the number of teeth by novel transcription factor Epiprofin in tooth germ formation and development. Our analysis of Epfn KO mice revealed that Epiprofin promotes the transitory cell proliferative activities in keratinocyte as well as dental epithelial cells. In Epfn KO mice, keratinocytes and dental epithelial cells keep migrating into dental mesenchyme due to the decrease of proliferation activity and apoptosis of cells. In addition, Epfn contributes to activate the cell proliferation of Transit Amplifying cells produced from stem cells through Notch signaling. Furthermore Epfn exerts a critical role in cell fate determination of dental epithelial cells and epithelial stem cells.

  14. Development of the dental pulp stem cell differentiation derivative method using GDF-5

    MARUYA Yuriko, NAKAMURA Takashi, IWAMOTO Tsutomu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    2012/04/01 - 2015/03/31

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    Growth/Differentiation Factor-5 (GDF-5) is a member of the bone morphogenetic protein and promotes formation of bones and cartilages. It has been reported that GDF-5 gene is expressed in oral region. However, the roles of GDF-5 in the differentiation of dental pulp cells are unknown. Thus, we investigated the effect of GDF-5 on the differentiation of dental pulp stem cell. GDF-5 promoted production of molecules characteristic for odontoblastic phenotype.And gene expression of VEGF increased in the presence of GDF-5. These findings suggested that GDF-5 promotes differentiation of dental pulp stem cell to odontblast.In addition, possibility to become the effective molecules in reproducing pulp tissue from dental pulp stem cell was suggested .

  15. Development of a novel cell based regenerative therapy

    NAKAMURA Takashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    The balance of sustainable production of maintenance and regenerating tissue component cells of stem cells is crucial in the tissue regeneration therapy with stem cells. Vascular endothelial cells, there is microenvironment where keep the production balance and maintain progenitor cells of the hematopoietic stem cells in the bone marrow tissues are called the vascular niche. However, the detail mechanisms of the functional maintenance in vascular niche by various factors secreted from vascular endothelial cells are still not fully understood. We focus on Wnt and Hedgehog signaling which is believed to also play an important role in the vascular niche and analyze both molecular signals in the maintenance of stem cells and cell fate determination in undifferentiated mesenchymal cells. We identified novel small molecules, which promote osteoblastic differentiation in mesenchymal stem cells effectively.

  16. Analysis of multiple odontoma in the patients

    KIKUIRI Takashi, YOSHIMURA Yoshitaka, FUKUMOTO Satoshi, NAKAMURA Takashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Hokkaido University

    2012/04/01 - 2014/03/31

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    Odontomas are hamartoma composed of a mixture of odontogenic hard and soft tissues. Odontomas are the most common of the odontogenic tumors in the jaw. However it is a rare case that multiple odontoma occurs in the same patient. Here we report two patients of multiple odontomas. These two patients have both of the compound and complex type odontoma at the root apical of all molars. Microscopic examination showed the unusual structure of dentin tubes and cementum of not only odontoma but also extracted tooth. Because the clinical features of these two patients with multiple odontoma seem to be relatively consistent, we performed the gene analysis using CGH array. These data suggested that the structural abnormalities of the tooth and the occurrence of multiple odontomas are caused by specific genes.

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Media Coverage 2

  1. 人工的に歯のエナメル質を形成することに成功

    2016/11/07

    Type: Other

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    国立大学法人東北大学は、歯の発生やかたちの制御に関わる分子の役割を解明する過程で、エナメル質の形成のマスター遺伝子の同定と機能解析に成功し、どのように歯のエナメルが作られ、また、歯のかたちを制御しているのかを明らかにしました。 本研究は東北大学歯学研究科歯科薬理学分野の中村卓史准教授、小児発達歯科学分野の福本敏教授らと、米国国立衛生研究所との共同研究による成果です。 私たちの歯の最外層はエナメル質という構造で守られており、体の中で最も硬い組織です。骨や軟骨などの硬組織と異なり、歯のエナメル質は皮膚の上皮細胞や毛や爪と同じ歯原性上皮細胞とよばれる上皮細胞によって形成されます。また、歯の生える場所に応じて変化する歯の歯冠や歯根のかたちは、この歯原性上皮細胞が制御しています。 本研究では、転写因子の1つであるエピプロフィンをマウスの全身の上皮細胞に発現するような遺伝子操作したマウス（K5-Epfnマウス）を

  2. 皮膚、毛などの増殖や分化 同一遺伝子関与

    河北新報他

    2014/11/25

    Type: Newspaper, magazine

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    再生医療・がん治療へ道 東北大大学院歯学研究科の中村卓史准教授（再生医療）、福本敏教授（小児歯科）らの研究グループは、歯、皮膚、毛など「上皮系器官」の細胞の増殖や分化に同じ遺伝子が関わっていることを突き止めた。形や大きさの制御が難しかった歯や皮膚の再生医療に道を開くとともに、がんの診断や治療への応用が期待されるという。 研究グループは、歯などの形成に関わる遺伝子「エピプロフィン」を欠損させたマウスやヒトの細胞を用い、上皮系器官ができる過程を調べた。 歯や皮膚の元となる組織幹細胞は分裂して前駆細胞になり、増殖、各器官への分化、増殖停止へと段階が進む。 これまで増殖、分化、増殖停止は別々の現象と考えられていたが、いずれの段階でもエピプロフィンが、作用する分子を変えながら働いていることが分かった。 組織幹細胞はあらゆる細胞を作りだす万能性を持っており、再生医療への応用が注目されている。エピプ