Details of the Researcher

PHOTO

Osamu Suzuki
Section
Graduate School of Dentistry
Job title
Professor
Degree

  • 医学博士（東北大学）

Committee Memberships 7

  • 日本再生医療学会　代議員

    2018/11 - Present

  • 日本バイオマテリアル学会　常任理事

    2018/04 - Present

  • 日本歯科理工学会　副理事長，Dental Materials Journal編集長

    2018/04 - 2020/03

  • 日本バイオマテリアル学会　評議員

    2006/04 - 2011/03

  • 日本歯科理工学会　評議員

    2005/08 - 2011/03

  • 日本再生医療学会　評議員

    2006/11 - 2009/11

  • 日本歯科基礎医学会　評議員

    2004/10 - 2009/03

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Professional Memberships 6

  • 東北骨代謝研究会(2005/02-2010/01 幹事)

  • 日本バイオマテリアル学会・東北支部会(2006/12-2011/11 支部委員)

  • 日本再生医療学会(2006/11-2009/11 評議員)

  • 日本バイオマテリアル学会(2006/04-2011/03 評議員)

  • 日本歯科理工学会(2005/08-2011/03 評議員)

  • 日本歯科基礎医学会(2004/10-2009/03 評議員)

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Research Interests 5

  • 骨再生

  • バイオミネラリゼーション

  • 生体材料学

  • マイクロ・ナノ技術

  • 組織工学

Research Areas 7

  • Life sciences / Surgical dentistry /

  • Life sciences / Prosthodontics /

  • Life sciences / Orthopedics /

  • Life sciences / Regenerative dentistry and dental engineering /

  • Nanotechnology/Materials / Basic physical chemistry / Biomineralization and tissue engineering of bone and tooth

  • Life sciences / Biomaterials / Biomaterial

  • Life sciences / Biomedical engineering / Biomaterial

Awards 6

  1. Fellow, Biomaterials Science and Engineering (FBSE)

    2020/12　International Union of Societies for Biomaterial Science and Engineering (IUSBSE)

  2. 日本バイオマテリアル学会賞（科学）

    2015/11　日本バイオマテリアル学会　リン酸八カルシウム骨補填材の開発と骨伝導機序の解明

  3. 平成25年度科研費審査委員表彰

    2013/11　日本学術振興会

  4. 第23回エンジニアリング功労者賞（特別テーマ）.

    2003/07/10　(財)エンジニアリング振興協会　わが国エンジニアリング産業の新たな事業展開に先駆的な例を示したプロジェクトの特別枠

  5. 日揮（株）社員表彰

    2003/01　日揮株式会社　コーカー加熱炉設計手法の確立（加熱炉管コーク付着抑制技術の確立）

  6. 日揮（株）開発努力賞

    1998/02　日揮株式会社　純蒸気配管･機器の表面処理技術の開発（サニタリー配管汚れ付着防止メカニズムの解明）

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Papers 347

  1. Effect of Octacalcium Phosphate on Osteogenic Differentiation of Induced Pluripotent Stem Cells in a 3D Hybrid Spheroid Culture

    Yuki Sugai, Ryo Hamai, Yukari Shiwaku, Takahisa Anada, Kaori Tsuchiya, Tai Kimura, Manami Tadano, Kensuke Yamauchi, Tetsu Takahashi, Hiroshi Egusa, Osamu Suzuki

    Biomimetics　10　(4)　205-205　2025/03/26

    Publisher: MDPI AG

    DOI： 10.3390/biomimetics10040205 　

    eISSN： 2313-7673

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    Octacalcium phosphate (OCP) has been shown to exhibit an osteogenic property and, therefore, has been utilized recently as a bone substitute, clinically. However, the stimulatory capacity for induced pluripotent stem (iPS) cells is not known. This study investigated whether OCP enhances osteoblastic differentiation of three-dimensionally cultured spheroids of iPS cells compared to hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP). Mouse iPS cells were mixed with smaller (less than 53 μm) or larger (300–500 μm) sizes of calcium phosphate (CaP) granules and cultured in a laboratory-developed oxygen-permeable culture chip under minimizing hypoxia for up to 21 days. Osteoblastic differentiation was estimated by the cellular alkaline phosphatase (ALP) activities. The degree of supersaturation (DS) with respect to CaP phases was determined from the media chemical compositions. Incubated CaP materials were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The culture promoted well the formation of hybrid spheroids of CaP materials and iPS cells regardless of the type of materials and their granule sizes. The ALP activity of OCP was about 1.5 times higher than that of β-TCP and HA in smaller granule sizes. FTIR, XRD, and DS analyses showed that larger OCP granules tended to hydrolyze to HA slightly faster than smaller granules with time while HA and β-TCP materials tended to remain unchanged. In conclusion, the results suggest that OCP enhances the osteogenic differentiation of iPS cells more than HA and β-TCP through a mechanism of hydrolyzing to HA. This inherent material property of OCP is essential for enhancing the osteoblastic differentiation of iPS cells.

  2. Enhanced osteogenic capacity of octacalcium phosphate involving adsorption of stromal-derived factor-1 in a standardized defect of a rat femur. International-journal

    Ryuichi Kanabuchi, Ryo Hamai, Yu Mori, Soshi Hamada, Yukari Shiwaku, Yuko Sai, Kaori Tsuchiya, Toshimi Aizawa, Osamu Suzuki

    Journal of materials science. Materials in medicine　36　(1)　23-23　2025/02/28

    DOI： 10.1007/s10856-025-06872-9 　

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    This study investigated whether octacalcium phosphate (OCP) enhances bone regeneration through its synergistic effect with stromal-derived factor-1 (SDF-1). Recombinant SDF-1 (0.5-5.0 μg) was combined with OCP granules through lyophilization. OCP/SDF-1 granules were implanted into a rat femoral standardized defect for 2 and 4 weeks and subjected to histomorphometry, C-X-C motif chemokine receptor 4 (CXCR4) and osteocalcin immunohistomorphometry, and tartrate-resistant acid phosphatase (TRAP) staining. Calcium-deficient hydroxyapatite (CDHA) was used as a control for in vitro analyses. Mesenchymal stem cell (MSC) migration was estimated using a Transwell system with OCP/SDF-1. SDF-1 release from OCP/SDF-1 into the supernatant was determined without cells. SDF-1 adsorption in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer onto OCP, the chemical structure of OCP immersed in the medium using Fourier transform infrared spectroscopy, and the degree of supersaturation of the medium were determined. Bone regeneration and OCP degradation were enhanced the most by 1.0 μg of OCP/SDF-1 at 2 weeks after implantation by CT analysis and increasing CXCR4-positive, osteocalcin-positive, and TRAP-positive cells accumulation around the OCP. MSC migration increased until 48 h in the following order: SDF-1 only, CDHA/SDF-1, and OCP/SDF-1, with the greatest effect with 1.0 μg of SDF-1 than from OCP. CDHA promoted a greater release than OCP at 48 h. The physicochemical analyses indicated that SDF-1 interacted with OCP through Freundlich-type adsorption and that the adsorption controlled SDF-1 release from OCP during the hydrolysis into CDHA. Therefore, leveraging its molecular affinity for the OCP surface, OCP/SDF-1 facilitates MSC migration and enhances bone formation by ensuring the controlled, sustained release of SDF-1 from OCP.

  3. Adsorption of Serum Fetuin onto Octacalcium Phosphate and Its Relation to Osteogenic Property

    Yuki Tsuboi, Ryo Hamai, Kyosuke Okuyama, Kaori Tsuchiya, Yukari Shiwaku, Kensuke Yamauchi, Osamu Suzuki

    International Journal of Molecular Sciences　26　(3)　1391-1391　2025/02/06

    Publisher: MDPI AG

    DOI： 10.3390/ijms26031391 　

    eISSN： 1422-0067

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    This study aimed to investigate how the chemical elements in relation to octacalcium phosphate (OCP) hydrolysis affect the osteoblastic differentiation in the presence of serum fetuin. The adsorption of fetuin onto OCP was examined in buffers having different degrees of supersaturation (DS) with respect to OCP and hydroxyapatite (HA) at pH 7.4 and 37 °C. The osteoblastic differentiation of mesenchymal stem cells (MSCs) was evaluated in cultures with OCP and 0 to 0.8 mg/mL of fetuin. The amount of fetuin adsorbed increased with increasing DS in the buffer. In the MSC culture, the coexistence of OCP and 0.2–0.4 mg/mL of fetuin close to serum level increased alkaline phosphatase activity; however, the activity was suppressed by 0.2–0.8 mg/mL of fetuin. Transmission electron microscopy revealed de novo crystal formation on OCP in supersaturated buffer and culture media with respect to OCP and HA at lower fetuin concentrations. Infrared spectroscopy and DS estimation indicate that the hydrolysis of OCP with de novo apatite formation was promoted in the culture media at 0.2–0.4 mg/mL of fetuin. These results suggest that OCP may promote osteoblastic differentiation if the suitable conditions are attained regarding the chemical elements and fetuin adsorption around OCP.

  4. Enhancement of the Cytokine Adsorption Capacity of Octacalcium Phosphate by Interaction-Controlled Glycosaminoglycan Modification to Promote Osteoblastic Differentiation

    Ryo Hamai, Kaori Tsuchiya, Osamu Suzuki

    Langmuir　40　(52)　27253-27269　2024/12/18

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.langmuir.4c03067 　

    ISSN： 0743-7463

    eISSN： 1520-5827

  5. Induced pluripotent stem cell-derived neural stem cells promote bone formation in mice with calvarial defects

    Yukari Shiwaku, Hiroko Okawa, Ikuro Suzuki, Susumu Sakai, Hiroshi Egusa, Osamu Suzuki

    Acta Biomaterialia　188　93-102　2024/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.actbio.2024.08.054 　

    ISSN： 1742-7061

  6. Development of triple-functionalized calcium phosphate nanoparticles as an advanced drug delivery system for bone tissue repair

    Taichi Tenkumo, Benedikt Kruse, Kathrin Kostka, Viktoriya Sokolova, Toru Ogawa, Nobuhiro Yoda, Oleg Prymak, Osamu Suzuki, Keiichi Sasaki, Matthias Epple

    Regenerative Therapy　25　49-60　2024/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.reth.2023.11.010 　

    ISSN： 2352-3204

  7. Efficacy of Octacalcium Phosphate/Gelatin (OCP/Gel) Composite Implantation for Miniature Swine Lumbar Interbody Fusion

    Yu Mori, Ryo Hamai, Ryuichi Kanabuchi, Takahiro Onoki, Kohei Takahashi, Ko Hashimoto, Toshimi Aizawa, Osamu Suzuki

    The Tohoku Journal of Experimental Medicine　2024

    Publisher: Tohoku University Medical Press

    DOI： 10.1620/tjem.2024.j138 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  8. Effects of degradation products from gelatin spongy scaffolds on angio-osteogenic capacity

    Takanori Hatakeyama, Ryo Hamai, Yukari Shiwaku, Takahisa Anada, Susumu Sakai, Tomoya Sato, Kazuyoshi Baba, Keiichi Sasaki, Osamu Suzuki

    Science and Technology of Advanced Materials　2023/11/03

    Publisher: Informa UK Limited

    DOI： 10.1080/14686996.2023.2277675 　

    ISSN： 1468-6996

    eISSN： 1878-5514

  9. Adsorption behavior and osteoblastic cellular activity of metronidazole molecule with octacalcium phosphate and hydroxyapatite materials

    Danupong Chaiariyakul, Ryo Hamai, Yukari Shiwaku, Kaori Tsuchiya, Osamu Suzuki

    Journal of Drug Delivery Science and Technology　89　105046-105046　2023/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.jddst.2023.105046 　

    ISSN： 1773-2247

  10. ラット大腿骨皮質骨欠損におけるOCP/Gel,Gel,β-TCPの骨修復能の検討

    森 優, 金淵 龍一, 濱井 瞭, 土屋 香織, 馬場 一慈, 濱田 壮志, 相澤 俊峰, 鈴木 治

    日本バイオマテリアル学会大会予稿集　45回　143-143　2023/10

    Publisher: 日本バイオマテリアル学会

  11. ケモカイン担持リン酸八カルシウムのラット長管骨欠損修復能の評価

    金淵 龍一, 濱井 瞭, 塩飽 由香利, 森 優, 土屋 香織, 蔡 優広, 濱田 壮志, 栗山 恭明, 原田 健登, 相澤 俊峰, 鈴木 治

    日本バイオマテリアル学会大会予稿集　45回　270-270　2023/10

    Publisher: 日本バイオマテリアル学会

  12. リン酸オクタカルシウム/ゼラチン多孔質複合体のラット大腿骨両側皮質骨欠損モデルに対する骨修復能の放射線学的・組織学的評価

    金淵 龍一, 森 優, 濱井 瞭, 土屋 香織, 宮武 尚史, 馬場 一慈, 濱田 壮志, 栗山 恭明, 原田 健登, 相澤 俊峰, 鈴木 治

    日本整形外科学会雑誌　97　(8)　S1617-S1617　2023/08

    Publisher: (公社)日本整形外科学会

    ISSN： 0021-5325

  13. リン酸オクタカルシウム/ゼラチン多孔質複合体のラット大腿骨両側皮質骨欠損モデルに対する骨修復能の放射線学的・組織学的評価

    金淵 龍一, 森 優, 濱井 瞭, 土屋 香織, 宮武 尚史, 馬場 一慈, 濱田 壮志, 栗山 恭明, 原田 健登, 相澤 俊峰, 鈴木 治

    日本整形外科学会雑誌　97　(8)　S1617-S1617　2023/08

    Publisher: (公社)日本整形外科学会

    ISSN： 0021-5325

  14. CLOSE UP! インプラント治療のためのコラーゲン使用人工骨「ボナーク」の有効な使い方のポイント

    川井 忠, 鎌倉 慎治, 鈴木 治

    日本歯科評論　83　(5)　83-94　2023/05

    Publisher: (株)ヒョーロン・パブリッシャーズ

    ISSN： 0289-0909

  15. The material design of octacalcium phosphate bone substitute: increased dissolution and osteogenecity. International-journal

    Osamu Suzuki, Ryo Hamai, Susumu Sakai

    Acta biomaterialia　158　1-11　2023/03/01

    DOI： 10.1016/j.actbio.2022.12.046 　

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    Octacalcium phosphate (OCP) has been advocated as a precursor of bone apatite crystals. Recent studies have shown that synthetic OCP exhibits highly osteoconductive properties as a bone substitute material that stems from its ability to activate bone tissue-related cells, such as osteoblasts, osteocytes, and osteoclasts. Accumulated experimental evidence supports the proposition that the OCP-apatite phase conversion under physiological conditions increases the stimulatory capacity of OCP. The conversion of OCP progresses by hydrolysis toward Ca-deficient hydroxyapatite with Ca2+ ion incorporation and inorganic phosphate ion release with concomitant increases in the solid Ca/P molar ratio, specific surface area, and serum protein adsorption affinity. The ionic dissolution rate during the hydrolysis reaction was controlled by introducing a high-density edge dislocation within the OCP lattice by preparing it through co-precipitation with gelatin. The enhanced dissolution intensifies the material biodegradation rate and degree of osteogenecity of OCP. Controlling the biodegradation rate relative to the dissolution acceleration may be vital for controlling the osteogenecity of OCP materials. This study investigates the effects of the ionic dissolution of OCP, focusing on the structural defects in OCP, as the enhanced metastability of the OCP phase modulates biodegradability followed by new bone formation. STATEMENT OF SIGNIFICANCE: Octacalcium phosphate (OCP) is recognized as a highly osteoconductive material that is biodegradable by osteoclastic resorption, followed by new bone formation by osteoblasts. However, if the degradation rate of OCP is increased by maintaining the original osteoconductivity or acquiring a bioactivity better than its current properties, then early replacement with new bone can be expected. Although cell introduction or growth factor addition by scaffold materials is the standard method for tissue engineering, material activity can be augmented by introducing dislocations into the lattice of the OCP. This review article summarizes the effects of introducing structural defects on activating OCP, which was obtained by co-precipitation with gelatin, as a bone substitute material and the mechanism of improved bone replacement performance.

  16. Effect of Octacalcium Phosphate Crystals on the Osteogenic Differentiation of Tendon Stem/Progenitor Cells In Vitro

    Xianchen Liu, Yukari Shiwaku, Ryo Hamai, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki

    International Journal of Molecular Sciences　24　(2)　1235-1235　2023/01/08

    Publisher: MDPI AG

    DOI： 10.3390/ijms24021235 　

    eISSN： 1422-0067

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    Synthetic octacalcium phosphate (OCP) activates bone tissue-related cells, such as osteoblasts, osteoclasts, and vascular endothelial cells. However, the effect of OCP on tendon-related cell activation remains unknown. This study examined the response of rat tendon stem/progenitor cells (TSPCs) to OCP and related calcium phosphate crystals in vitro. TSPCs were cultured with OCP and Ca-deficient hydroxyapatite (CDHA) obtained from the original OCP hydrolysis to assess the activity of alkaline phosphatase (ALP) and the expression of osteogenesis-related genes. Compared with CDHA, the effect of OCP on promoting the osteogenic differentiation of TSPCs was apparent: the ALP activity and mRNA expression of RUNX2, Col1a1, OCN, and OPN were higher in OCP than in CDHA. To estimate the changes in the chemical environment caused by OCP and CDHA, we measured the calcium ion (Ca2+) and inorganic phosphate (Pi) ion concentrations and pH values of the TSPCs medium. The results suggest that the difference in the osteogenic differentiation of the TSPCs is related to the ionic environment induced by OCP and CDHA, which could be related to the progress of OCP hydrolysis into CDHA. These results support the previous in vivo observation that OCP has the healing function of rabbit rotator cuff tendon in vivo.

  17. Impact of Octacalcium Phosphate/Gelatin (OCP/Gel) Composite on Bone Repair in Refractory Bone Defects

    Yu Mori, Ryo Hamai, Toshimi Aizawa, Osamu Suzuki

    The Tohoku Journal of Experimental Medicine　2023

    Publisher: Tohoku University Medical Press

    DOI： 10.1620/tjem.2023.j040 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  18. Osteogenic capacity of octacalcium phosphate involving macrophage polarization. International-journal

    Linghao Xiao, Yukari Shiwaku, Ryo Hamai, Kazuyoshi Baba, Kaori Tsuchiya, Satoshi Imazato, Keiichi Sasaki, Osamu Suzuki

    Journal of biomedical materials research. Part A　2022/12/27

    DOI： 10.1002/jbm.a.37484 　

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    Previous research has found that octacalcium phosphate (OCP) increases macrophage accumulation and alters the initial inflammatory response. However, the role of the immune response induced by OCP in osteogenesis remains unknown. This study investigated the behavior of macrophages and bone regeneration capacity during the early inflammatory stage of OCP-mediated osteogenesis. To assess the change in macrophage polarization and osteogenic capacity, we used a standardized rat defect model filled with OCP or calcium-deficient hydroxyapatite (CDHA)-a material obtained through the hydrolysis of the original OCP. OCP or CDHA granules were incubated with RAW264 cells for 5 days to investigate the effect of physicochemical characteristics on macrophage cytokine/chemokine expression in vitro. Our in vivo results show that due to the OCP implantation, macrophages in the rat tibial defect area tend to polarize to the M2 phenotype (anti-inflammatory) and inhibit the formation of the M1 phenotype (pro-inflammatory). In comparison to CDHA, OCP exhibited superior bone regeneration potential due to its rapid promotion of cortical bone healing and stimulation of macrophage-related growth factors. Furthermore, our in vitro results have shown that OCP regulates the expression of macrophage chemokines over time. Compared to incubation with CDHA, incubation with OCP caused changes in the ionic microenvironment. These findings suggest that the OCP-mediated macrophage polarization and secretion profile not only regulate immune function but also positively affect osteogenesis.

  19. マウス骨髄由来間葉系幹細胞の分化と骨再生に及ぼすリン酸八カルシウムの効果

    奥山 喬介, 塩飽 由香利, 濱井 瞭, 溝口 利英, 土屋 香織, 鈴木 治

    日本バイオマテリアル学会大会予稿集　44回　182-182　2022/11

    Publisher: 日本バイオマテリアル学会

  20. リン酸八カルシウム周囲の間葉系幹細胞の局在と分化

    奥山 喬介, 塩飽 由香利, 濱井 瞭, 溝口 利英, 土屋 香織, 高橋 哲, 鈴木 治

    日本口腔科学会雑誌　71　(2)　122-122　2022/07

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  21. リン酸八カルシウムに含有された微量無機イオンによる血管新生と骨形成の関係について

    高山 慎騎, 濱井 瞭, 塩飽 由香利, 黒羽根 壮, 土屋 香織, 高橋 哲, 鈴木 治

    日本口腔科学会雑誌　71　(2)　122-122　2022/07

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  22. Octacalcium Phosphate/Gelatin Composite (OCP/Gel) Enhances Bone Repair in a Critical-Sized Transcortical Femoral Defect Rat Model

    Soshi Hamada, Yu Mori, Yukari Shiwaku, Ryo Hamai, Kaori Tsuchiya, Kazuyoshi Baba, Itsuki Oizumi, Ryuichi Kanabuchi, Naohisa Miyatake, Toshimi Aizawa, Osamu Suzuki

    Clinical Orthopaedics & Related Research　Publish Ahead of Print　(10)　2043-2055　2022/05/30

    Publisher: Ovid Technologies (Wolters Kluwer Health)

    DOI： 10.1097/corr.0000000000002257 　

    ISSN： 0009-921X

    eISSN： 1528-1132

  23. リン酸八カルシウムと骨髄間葉系幹細胞の挙動

    奥山 喬介, 塩飽 由香利, 濱井 瞭, 高橋 哲, 鈴木 治

    日本歯科理工学会誌　41　(Special Issue79)　34-34　2022/05

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  24. 微量銅イオン含有により誘導される血管新生がリン酸八カルシウムによる骨再生に与える影響について

    高山 慎騎, 濱井 瞭, 塩飽 由香利, 黒羽根 壮, 高橋 哲, 鈴木 治

    日本歯科理工学会誌　41　(Special Issue79)　33-33　2022/05

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  25. Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity

    Ryo Hamai, Susumu Sakai, Yukari Shiwaku, Takahisa Anada, Kaori Tsuchiya, Takuya Ishimoto, Takayoshi Nakano, Osamu Suzuki

    Applied Materials Today　26　101279-101279　2022/03

    DOI： 10.1016/j.apmt.2021.101279 　

    ISSN： 2352-9407

  26. Differentiation of committed osteoblast progenitors by octacalcium phosphate compared to calcium-deficient hydroxyapatite in Lepr-cre/Tomato mouse tibia. International-journal

    Kyosuke Okuyama, Yukari Shiwaku, Ryo Hamai, Toshihide Mizoguchi, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki

    Acta biomaterialia　142　332-344　2022/02/17

    DOI： 10.1016/j.actbio.2022.02.016 　

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    This study aimed to investigate the accumulation and differentiation of mesenchymal stem cells (MSCs) around octacalcium phosphate (OCP) compared with those around calcium-deficient hydroxyapatite (CDHA), a material obtained through hydrolysis of the original OCP. Leptin receptor (Lepr)-expressing bone marrow-derived MSCs around the OCP and CDHA were pursued utilizing genetically modified Lepr-cre/Tomato mice. OCP and CDHA granules were implanted into the tibia defect of the mice for 10 weeks and subjected to histomorphometric and immunohistochemical analyses. The structural properties of OCP and CDHA after inoculation into mouse subcutaneous tissue (until 4 weeks) or culture mediums (14 days) were analyzed using physicochemical techniques. In vitro osteoblastic differentiation of primary MSCs was examined with the materials for 14 days. While Lepr-cre/Tomato positive cells (red) accumulated around both OCP and CDHA, Lepr and osteocalcin double-positive osteoblastic cells (yellow) were significantly more abundant around OCP than around CDHA in the early implantation period. OCP enhanced the osteoblastic differentiation of MSCs more than CDHA in vitro. Physicochemical techniques, including selected area electron diffraction, provided evidence that OCP tended to convert to the apatitic phase in the tested physiological environments. The higher osteoconductivity of OCP originated from a capacity-enhancing osteoblastic differentiation of committed osteoblast progenitors in bone marrow accompanied by OCP hydrolysis. STATEMENT OF SIGNIFICANCE: : Mesenchymal stem cells (MSCs) play a key role in bone regeneration by differentiating MSCs into osteoblasts. Calcium phosphates have been widely applied as bone substitute materials, and OCP has a better ability to promote osteoblast differentiation of MSCs than that of HA in vitro. However, it is not clear how MSCs accumulate in the bone marrow and differentiate into osteoblasts during bone regeneration in vivo. In this study, we focused on the leptin receptor, a marker of bone marrow-derived MSCs. Using genetically modified mice labeled with the red fluorescent protein Tomato, we observed the accumulation of MSCs around calcium phosphates implanted in tibia bone defects and their differentiation into osteoblasts.

  27. Angio-osteogenic capacity of octacalcium phosphate co-precipitated with copper gluconate in rat calvaria critical-sized defect

    Shinki Koyama, Ryo Hamai, Yukari Shiwaku, Tsuyoshi Kurobane, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki

    Science and Technology of Advanced Materials　2022/02/01

    Publisher: Informa UK Limited

    DOI： 10.1080/14686996.2022.2035193 　

    ISSN： 1468-6996

    eISSN： 1878-5514

  28. Proteomic identification of serum proteins to induce osteoconductivity of hydroxyapatite Peer-reviewed

    Yunting WANG, Yutaka YOSHIDA, Junichi KAMIIE, Yukari SHIWAKU, Osamu SUZUKI, Maiko FURUYA, Kotone YOKOTA, Hiroyasu KANETAKA, Taishi YOKOI, Masakazu KAWASHITA

    Dental Materials Journal　40　(6)　1428-1436　2021/11/25

    Publisher: Japanese Society for Dental Materials and Devices

    DOI： 10.4012/dmj.2021-120 　

    ISSN： 0287-4547

    eISSN： 1881-1361

  29. リン酸八カルシウムによる骨再生における間葉系幹細胞の役割に関する研究

    奥山 喬介, 塩飽 由香利, 濱井 瞭, 溝口 利英, 高橋 哲, 鈴木 治

    日本バイオマテリアル学会大会予稿集　43回　JP3-07　2021/11

    Publisher: 日本バイオマテリアル学会

  30. リン酸八カルシウムによる骨再生における血管新生の役割について

    高山 慎騎, 濱井 瞭, 塩飽 由香利, 黒羽根 壮, 土屋 香織, 高橋 哲, 鈴木 治

    日本バイオマテリアル学会大会予稿集　43回　JP1-07　2021/11

    Publisher: 日本バイオマテリアル学会

  31. Macrophage Polarization Related to Crystal Phases of Calcium Phosphate Biomaterials. International-journal

    Linghao Xiao, Yukari Shiwaku, Ryo Hamai, Kaori Tsuchiya, Keiichi Sasaki, Osamu Suzuki

    International journal of molecular sciences　22　(20)　2021/10/19

    DOI： 10.3390/ijms222011252 　

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    Calcium phosphate (CaP) materials influence macrophage polarization during bone healing. However, the effect of the crystal phase of CaP materials on the immune response of bone remains unclear. In this study, the effect of the crystal phases of CaP materials on the regulation of macrophage polarization was investigated. Human THP-1 cells and mouse RAW 264 cells were cultured with octacalcium phosphate (OCP) and its hydrolyzed form Ca-deficient hydroxyapatite to assess the expression of pro-inflammatory M1 and anti-inflammatory M2 macrophage-related genes. OCP inhibited the excessive inflammatory response and switched macrophages to the anti-inflammatory M2 phenotype, which promoted the expression of the interleukin 10 (IL10) gene. In contrast, HL stimulated an excessive inflammatory response by promoting the expression of pro-inflammatory M1 macrophage-related genes. To observe changes in the microenvironment induced by OCP and HL, inorganic phosphate (Pi) and calcium ion (Ca2+) concentrations and pH value in the medium were measured. The expression of the pro-inflammatory M1 macrophage-related genes (tumor necrosis factor alpha (TNFα) and interlukin 1beta (IL1β)) was closely related to the increase in ion concentration caused by the increase in the CaP dose. Together, these results suggest that the microenvironment caused by the crystal phase of CaP materials may be involved in the immune-regulation capacity of CaP materials.

  32. 顎骨再建の骨形態計測学的評価 リン酸オクタカルシウム・コラーゲン複合体の組織学的評価と臨床応用(Histological evaluation and clinical application of octacalcium phosphate and collagen composite)

    高橋 哲, 松井 桂子, 鎌倉 慎治, 川井 忠, 鈴木 治

    Journal of Oral Biosciences Supplement　2021　74-74　2021/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  33. 顎骨再建の骨形態計測学的評価 リン酸オクタカルシウム・コラーゲン複合体の組織学的評価と臨床応用(Histological evaluation and clinical application of octacalcium phosphate and collagen composite)

    高橋 哲, 松井 桂子, 鎌倉 慎治, 川井 忠, 鈴木 治

    Journal of Oral Biosciences Supplement　2021　74-74　2021/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  34. Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area

    Yukari Shiwaku, Ryo Hamai, Shinichi Sato, Susumu Sakai, Kaori Tsuchiya, Kazuyoshi Baba, Tetsu Takahashi, Osamu Suzuki

    International Journal of Molecular Sciences　22　(18)　9770-9770　2021/09/09

    Publisher: MDPI AG

    DOI： 10.3390/ijms22189770 　

    eISSN： 1422-0067

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    The microstructure of biomaterials influences the cellular and biological responses in the bone. Octacalcium phosphate (OCP) exhibits higher biodegradability and osteoconductivity than hydroxyapatite (HA) during the conversion process from OCP to HA. However, the effect of the microstructure of OCP crystals on long tubular bones has not been clarified. In this study, two types of OCPs with different microstructures, fine-OCP (F-OCP) and coarse-OCP (C-OCP), were implanted in rat tibia for 4 weeks. F-OCP promoted cortical bone regeneration compared with C-OCP. The osteoclasts appearance was significantly higher in the C-OCP group than in the control group (defect only) at 1-week post-implantation. To investigate whether the solubility equilibrium depends on the different particle sizes of OCPs, Nano-OCP, which consisted of nanometer-sized OCPs, was prepared. The degree of supersaturation (DS) tended to decrease modestly in the order of C-OCP, F-OCP, and Nano-OCP with respect to HA and OCP in Tris-HCl buffer. F-OCP showed a higher phosphate ion concentration and lower calcium ion concentration after immersion in the buffer than C-OCP. The crystal structures of both OCPs tended to be converted to HA by rat abdominal implantation. These results suggest that differences in the microstructure of OCPs may affect osteoclastogenesis and result in osteoconductivity of this material in long tubular bone by altering dissolution behavior.

  35. A follow-up study on the clinical outcomes of alveolar reconstruction using octacalcium phosphate granules and atelocollagen complex

    Toshiro Kibe, Aya Iino-Maeda, Tetsu Takahashi, Shinji Kamakura, Osamu Suzuki, Norifumi Nakamura

    Journal of Oral and Maxillofacial Surgery　2021/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.joms.2021.09.017 　

    ISSN： 0278-2391

  36. Involvement of distant octacalcium phosphate scaffolds in enhancing early differentiation of osteocytes during bone regeneration

    Shizu Saito, Ryo Hamai, Yukari Shiwaku, Tomoka Hasegawa, Susumu Sakai, Kaori Tsuchiya, Yuko Sai, Ryosuke Iwama, Norio Amizuka, Tetsu Takahashi, Osamu Suzuki

    Acta Biomaterialia　129　309-322　2021/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.actbio.2021.05.017 　

    ISSN： 1742-7061

  37. New Dental Material 新規骨補填材料 リン酸八カルシウム・コラーゲン複合体「ボナーク」について

    川井 忠, 高橋 哲, 松井 桂子, 鈴木 治, 鎌倉 慎治

    日本歯科評論　81　(5)　105-114　2021/05

    Publisher: (株)ヒョーロン・パブリッシャーズ

    ISSN： 0289-0909

  38. Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration

    Hisashi Ozaki, Ryo Hamai, Yukari Shiwaku, Susumu Sakai, Kaori Tsuchiya, Osamu Suzuki

    Science and Technology of Advanced Materials　2021/04/16

    Publisher: Informa UK Limited

    DOI： 10.1080/14686996.2021.1916378 　

    ISSN： 1468-6996

    eISSN： 1878-5514

  39. Impact of simultaneous hydrolysis of OCP and PLGA on bone induction of a PLGA-OCP composite scaffold in a rat femoral defect

    Itsuki Oizumi, Ryo Hamai, Yukari Shiwaku, Yu Mori, Takahisa Anada, Kazuyoshi Baba, Naohisa Miyatake, Soshi Hamada, Kaori Tsuchiya, Shin-nosuke Nishimura, Eiji Itoi, Osamu Suzuki

    Acta Biomaterialia　124　358-373　2021/04

    Publisher: Elsevier BV

    DOI： 10.1016/j.actbio.2021.01.048 　

    ISSN： 1742-7061

  40. ゼラチンスポンジが骨再生に及ぼす影響の検討

    畠山 高徳, 塩飽 由香利, 濱井 瞭, 穴田 貴久, 佐々木 啓一, 鈴木 治

    日本歯科理工学会誌　39　(Special Issue76)　6-6　2020/12

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  41. Effect of surface modification of Ti-6Al-4V alloy by electron cyclotron resonance plasma oxidation. Peer-reviewed

    Mayumi Oikawa, Hiroshi Masumoto, Naru Shiraishi, Yusuke Orii, Takahisa Anada, Osamu Suzuki, Keiichi Sasaki

    Dental materials journal　2020/10/15

    DOI： 10.4012/dmj.2020-051 　

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    Ti-6Al-4V alloy is used as biomaterials for dental and orthopedic implants because of their excellent biocompatibilities and mechanical properties. However, it is unclear that electron cyclotron resonance (ECR) plasma oxidation can create the oxide films on Ti-6Al-4V alloy surface, and this technique improves the ability of its osseointegration. The purpose of this study was to investigate the characteristics and calcification ability of the oxide films. X-ray diffraction (XRD) peaks of rutile phase were intensified with increasing the temperature. Scanning electron microscopy (SEM) images showed a crater-like structure, and bonding strengths between the substrate and oxide film reached a maximum at 400°C. Calcium phosphate (CaP) compounds after calcification process were identified as octacalcium phosphate (OCP) and precipitation amount was maximized at 400°C. The results suggested that the altered surface of Ti-6Al-4V alloy by ECR plasma oxidation might have the potential of accelerating the ability of its osseointegration through enhancement of OCP.

  42. Resorption analysis of deproteinized cancellous bovine bone. Peer-reviewed

    Masayuki Narukawa, Osamu Suzuki, Mitsuori Mayahara, Eisaku Imamura, Yoshitoki Takagi, Takashi Funae, Yuusuke Makino, Retsu Ohki, Masanori Nakamura

    Dental materials journal　39　(5)　760-765　2020/09/29

    DOI： 10.4012/dmj.2019-240 　

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    Commercially available deproteinized cancellous bovine bone (DPBB) has been indicated as non-absorbable bone filling materials. Stoichiometric hydroxyapatite (HA) was not resorbed by osteoclasts while calcium-deficient and carbonate-rich apatite converted from octacalcium (OCP hydrolysate) was resorbed by osteoclasts. We analyzed the chemical composition of DPBB and compared the tissue reactions around two materials after implanting into mouse bone marrow. X-ray diffraction analysis and Fourier transform infrared spectroscopy showed that DPBB was a carbonate-rich apatite. Micro-CT analysis indicated the massive bone formation on both materials at 2 weeks, then gradually resorbed. At 12 weeks, osteoclasts were directly attached to both materials. The ultrastructure of ruffled borders on DPBB was identical to osteoclasts resorbing normal bone while ruffled border on OCP hydrolysate showed irregular shape. These results indicated that DPBB was the absorbed material and that the structure of ruffled border of osteoclasts might be regulated by the size or orientation of HA.

  43. Comparative analysis of bovine serum albumin adsorption onto octacalcium phosphate crystals prepared using different methods. Peer-reviewed

    Kaori Tsuchiya, Ryo Hamai, Susumu Sakai, Osamu Suzuki

    Dental materials journal　39　(5)　883-891　2020/09/29

    DOI： 10.4012/dmj.2019-250 　

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    This study compared bovine serum albumin (BSA) adsorption onto octacalcium phosphate (OCP) materials prepared from two wet preparations in the absence (w-OCP) and presence (c-OCP) of gelatin. Raman spectroscopy was used to analyze the BSA adsorption onto OCPs in a 150 mM Tris-HCl buffer containing 0.5 mM calcium and inorganic phosphate (Pi) ions at pH 7.4 and at 37°C. The degree of supersaturation of the supernatants after the adsorption was determined by measuring the ion composition. The results showed that BSA adsorption onto w-OCP was higher than that for c-OCP. The calcium ion concentration of the supernatant decreased for both w-OCP and c-OCP, whereas the Pi ion concentration increased, approaching OCP equilibria at different saturation levels. BSA adsorbed even onto c-OCP, which included a small amount of gelatin during c-OCP preparation. These results indicate that the biodegradability of w-OCP and c-OCP may be modulated through interactions with serum proteins.

  44. ラット長管骨骨欠損モデルにおける新規骨補填材のリン酸八カルシウムと乳酸グリコール酸共重合体複合体による骨修復効果の検討

    大泉 樹, 森 優, 塩飽 由香利, 濱井 瞭, 馬場 一慈, 穴田 貴久, 井樋 栄二, 鈴木 治

    日本整形外科学会雑誌　94　(8)　S1917-S1917　2020/09

    Publisher: (公社)日本整形外科学会

    ISSN： 0021-5325

  45. Effect of Surrounding Chemical Environment on Adsorption and Accumulation of Serum Protein onto Octacalcium Phosphate Crystals

    Ryo HAMAI, Kaori TSUCHIYA, Osamu SUZUKI

    Journal of the Japan Society of Powder and Powder Metallurgy　67　(5)　258-263　2020/05/15

    Publisher: Japan Society of Powder and Powder Metallurgy

    DOI： 10.2497/jjspm.67.258 　

    ISSN： 0532-8799

    eISSN： 1880-9014

  46. リン酸八カルシウム結晶周囲の化学環境が血清タンパク質吸着と集積に及ぼす影響の検討 Invited Peer-reviewed

    濱井 瞭, 土屋 香織, 鈴木 治

    粉体および粉末冶金　67　258-263　2020/05/15

  47. ゼラチン多孔質足場材料がラット頭蓋冠骨欠損部に及ぼす骨再生能の評価

    畠山 高徳, 塩飽 由香利, 濱井 瞭, 穴田 貴久, 酒井 進, 佐々木 啓一, 鈴木 治

    日本歯科理工学会誌　39　(Special Issue75)　37-37　2020/04

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  48. OCP/SF2細胞スフェロイド複合体におけるエナメル芽細胞分化の評価

    ただ野 愛実, 塩飽 由香利, 濱井 瞭, 穴田 貴久, 鈴木 治

    日本歯科理工学会誌　39　(Special Issue75)　72-72　2020/04

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  49. Octacalcium phosphate bone substitute materials: Comparison between properties of biomaterials and other calcium phosphate materials. Peer-reviewed

    Osamu Suzuki, Yukari Shiwaku, Ryo Hamai

    Dental materials journal　39　(2)　187-199　2020/03/31

    DOI： 10.4012/dmj.2020-001 　

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    Octacalcium phosphate (OCP) is a material that can be converted to hydroxyapatite (HA) under physiological environments and is considered a mineral precursor to bone apatite crystals. The structure of OCP consists of apatite layers stacked alternately with hydrated layers, and closely resembles the structure of HA. The performance of OCP as a bone substitute differs from that of HA materials in terms of their osteoconductivity and biodegradability. OCP manifests a cellular phagocytic response through osteoclast-like cells similar to that exhibited by the biodegradable material β-tricalcium phosphate (β-TCP). The use of OCP for human cranial bone defects involves using its granule or composite form with one of the natural polymers, viz., the reconstituted collagen. This review article discusses the differences and similarities in these calcium phosphate (Ca-P)-based materials from the viewpoint of the structure and their material chemistry, and attempts to elucidate why Ca-P materials, particularly OCP, display unique osteoconductive property.

  50. Chemical stability-sensitive osteoconductive performance of octacalcium phosphate bone substitute in an ovariectomized rat tibia defect Peer-reviewed

    K Baba, Y Shiwaku, R Hamai, Y Mori, T Anada, K Tsuchiya, I Oizumi, N Miyatake, E Itoi, O Suzuki

    ACS Applied Bio Materials　3　(3)　1444-1458　2020/03

    DOI： 10.1021/acsabm.9b01091 　

    eISSN： 2576-6422

  51. Histomorphometric assessments of peri-implant bone around Ti-Nb-Sn alloy implants with low Young's modulus. Peer-reviewed

    Naru Shiraishi, Hiroshi Masumoto, Kenta Takahashi, Taichi Tenkumo, Takahisa Anada, Osamu Suzuki, Toru Ogawa, Keiichi Sasaki

    Dental materials journal　39　(1)　148-153　2020/01/31

    DOI： 10.4012/dmj.2018-376 　

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    Many β-Ti alloys have been developed for, and used in, medical devices because of the corrosion resistance, biocompatibility, and exceptionally low Young's modulus. The aim of the present study was to investigate the histomorphometric aspects of peri-implant bone around Ti-Nb-Sn alloy implants and compare them with those in the case of commercially pure Ti (Ti). Fluorescent morphological observations of ST-2 cells on the substrate were performed and bone morphogenesis around implants in rat femur was evaluated. There was no difference between the cell morphology on Ti and those on the Ti-Nb-Sn alloy during observation for 24 h. A comparison of the Ti-Nb-Sn alloy implant and the Ti implant showed no significant differences between the bone-to-implant contact ratios or the bone fractions. These results suggest that the biological adaptations with Ti-Nb-Sn implants during a healing period are similar to those with Ti. Ti-Nb-Sn is therefore suitable for use in dental implants.

  52. Erratum to: Compressive Force-Produced CCN2 Induces Osteocyte Apoptosis Through ERK1/2 Pathway: COMPRESSIVE FORCE-PRODUCED CCN2 INDUCES APOPTOSIS (Journal of Bone and Mineral Research, (2014), 29, 5, (1244-1257), 10.1002/jbmr.2115)

    Kenji Hoshi, Harumi Kawaki, Ichiro Takahashi, Nobuo Takeshita, Masahiro Seiryu, Sakhr A. Murshid, Taisuke Masuda, Takahisa Anada, Ryushi Kato, Hideki Kitaura, Osamu Suzuki, Teruko Takano-Yamamoto

    Journal of Bone and Mineral Research　2020

    DOI： 10.1002/jbmr.4169 　

    ISSN： 0884-0431

    eISSN： 1523-4681

  53. リン酸八カルシウム(OCP)材料-生理的石灰化促進型骨補填材- Invited

    鈴木 治

    日本歯科理工学会誌　67　258-263　2020

  54. Clinical study of octacalcium phosphate and collagen composite in oral and maxillofacial surgery Peer-reviewed

    Tadashi Kawai, Shinji Kamakura, Keiko Matsui, Masayuki Fukuda, Hiroshi Takano, Mitsuyoshi Iino, Shigeo Ishikawa, Hiromasa Kawana, Tomoya Soma, Eisaku Imamura, Hideki Kizu, Aya Michibata, Izumi Asahina, Keiichiro Miura, Norifumi Nakamura, Toshiro Kibe, Osamu Suzuki, Tetsu Takahashi

    Journal of Tissue Engineering　11　1-15　2020

    DOI： 10.1177/2041731419896449 　

    eISSN： 2041-7314

  55. 閉経後骨粗鬆症モデルラットにおけるリン酸八カルシウムの溶解性が骨新生に与える影響

    馬場 一慈, 塩飽 由香利, 濱井 瞭, 森 優, 穴田 貴久, 大泉 樹, 宮武 尚央, 土屋 香織, 井樋 栄二, 鈴木 治

    日本バイオマテリアル学会大会予稿集　41回　274-274　2019/11

    Publisher: 日本バイオマテリアル学会

  56. 骨粗鬆症モデルラットにおけるリン酸八カルシウムの骨新生の評価

    馬場 一慈, 塩飽 由香利, 穴田 貴久, 濱井 瞭, 森 優, 井樋 栄二, 鈴木 治

    日本整形外科学会雑誌　93　(8)　S1973-S1973　2019/09

    Publisher: (公社)日本整形外科学会

    ISSN： 0021-5325

  57. Degradation of extracellular matrices propagates calcification during development and healing in bones and teeth. International-journal Peer-reviewed

    Yasuyuki Sasano, Megumi Nakamura, Akiko Henmi, Hiroshi Okata, Osamu Suzuki, Atsuko Kayaba, Miyuki Mayanagi

    Journal of oral biosciences　61　(3)　149-156　2019/09

    DOI： 10.1016/j.job.2019.07.004 　

    ISSN： 1349-0079

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    BACKGROUND: Bone, dentin, and enamel are tissues formed through calcification, a process involving deposition of calcium phosphate minerals on extracellular organic matrices. Calcification, the underlying mechanism of which is unknown, is initiated with mineral deposition followed by advancing of the deposit and subsequent maturation of the mineral crystal. HIGHLIGHT: We have reviewed the current knowledge of how calcification proceeds during bone development, bone healing, and enamel and dentin development, based on reported studies. Previous studies reported by us and by other authors have suggested that degradation of some extracellular matrix (ECM) proteins is involved in calcification during bone and dentin development and bone healing in a manner similar to that previously reported for enamel development. CONCLUSION: The ECM proteins may inhibit mineral deposition and calcification, similar to the role of amelogenin during enamel development. The candidates for the amelogenin equivalents in bone and dentin have not been identified. Further studies are required to elucidate the regulatory mechanisms of bone and dentin calcification in light of specific ECM proteins that prevent calcification and enzymes that degrade these ECM proteins.

  58. Adsorption of Serum Albumin onto Octacalcium Phosphate in Supersaturated Solutions Regarding Calcium Phosphate Phases. International-journal Peer-reviewed

    Ryo Hamai, Kaori Tsuchiya, Osamu Suzuki

    Materials (Basel, Switzerland)　12　(14)　2333　2019/07/23

    DOI： 10.3390/ma12142333 　

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    Octacalcium phosphate (OCP) has been shown to enhance new bone formation, coupled with its own biodegradation, through osteoblasts and osteoclast-like cell activities concomitant with de novo hydroxyapatite (HA) formation and serum protein accumulation on its surface. However, the nature of the chemical environment surrounding OCP and how it affects its metabolism and regulates protein accumulation is unknown. The present study examined how the degree of supersaturation (DS) affects the bovine serum albumin (BSA) adsorption onto OCP in 150 mM Tris-HCl buffer at 37 °C and pH 7.4, by changing the Ca2+ ion concentration. The amount of BSA adsorbed onto OCP increased as the DS increased. In addition, the amount of newly formed calcium phosphate, which could be OCP, was increased, not only by increases in DS, but also at lower equilibrium concentrations of BSA. The increased adsorption capacity of BSA was likely related to the formation of calcium phosphate on the adsorbed OCP. Together the results suggested that the formation of new calcium phosphate crystals is dependent on both the DS value and the adsorbate protein concentration, which may control serum protein accumulation on the OCP surface in vivo.

  59. リン酸オクタカルシウム(OCP)により促進された骨再生における血管新生の観察

    黒羽根 壮, 塩飽 由香利, 穴田 貴久, 濱井 瞭, 土屋 香織, 飯久保 正弘, 高橋 哲, 鈴木 治

    日本口腔科学会雑誌　68　(2)　179-179　2019/07

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

  60. Effect of calcium phosphate phases affecting the crosstalk between osteoblasts and osteoclasts in vitro. International-journal Peer-reviewed

    Shiwaku Y, Tsuchiya K, Xiao L, Suzuki O

    Journal of biomedical materials research. Part A　107　(5)　1001-1013　2019/05

    DOI： 10.1002/jbm.a.36626 　

    ISSN： 1549-3296

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    Previous studies have reported that octacalcium phosphate (OCP) enhances osteoblast differentiation and osteoclast formation during the hydrolysis process to hydroxyapatite (HA). However, the crystal phases that affect the crosstalk between osteoclasts and osteoblasts are unknown, which should determine the bone substitute material's property of OCP. The present study was designed to investigate whether the chemical composition and crystal structure of calcium phosphates affect osteoclast formation and the osteoclast-osteoblast crosstalk. Biodegradable β-tricalcium phosphate (β-TCP) was used as the control material. Osteoclasts were cultured on HA/OCP or HA/TCP disks and their cellular responses were assessed. Both OCP and β-TCP had a similar ability to create multinucleated osteoclasts. However, OCP promoted the expression of complement component 3a (C3a), a positive coupling factor, in osteoclasts, whereas β-TCP enhanced that of EphrinB2 (EfnB2) and collagen triple helix repeat containing 1 (Cthrc1). During osteoclast culture, phosphate ions were released from the crystals, and OCP-HA conversion was advanced in HA/OCP mixtures and OCP. X-ray diffraction analysis revealed no remarkable changes in the crystal structures of HA/TCP mixtures and β-TCP before and after osteoclast culture. These results indicate that the distinct chemical environment induced by the calcium phosphate phases affects the crosstalk between osteoclasts and osteoblasts. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1001-1013, 2019.

  61. How the Japanese Society for Biomaterials established interdisciplinary studies in biomaterials science. International-journal Peer-reviewed

    Osamu Suzuki

    Journal of biomedical materials research. Part A　107　(5)　917-929　2019/05

    DOI： 10.1002/jbm.a.36629 　

    ISSN： 1549-3296

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    The Japanese Society for Biomaterials (JSB) was established in December 1978, aiming to develop and improve science and technology regarding materials used in living organisms and their application. Currently, JSB plays important roles as a domestic and international society serving as a center for interdisciplinary studies on biomaterial science and its applied research. Our academic activities are wide-ranging, from basic science and clinical practice to post-application medical devices including the following: holding academic lectures, granting academic and other awards, publishing academic journals, developing guidelines for medical devices and bioassays, conducting research associated with clinical trials and studies, and participating in the World Biomaterials Congress and overseas academic meetings on biomaterials. Through such activities, we have actively engaged professionally in diverse discussions and operations. Thanks to your support, JSB recently celebrated its 40th anniversary since establishment. Over the 40-year history, our predecessors worked hard to establish this interdisciplinary field spanning basic science (engineering, medical, biology, and others) and collaboration with companies. In addition, they contributed to society through the application of biomaterial technology. Their achievements made what we are today. To commemorate this anniversary, this report looks back at their achievements in order to use such information for further academic progress. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 917-929, 2019.

  62. ラット頭蓋冠骨欠損に埋入したリン酸オクタカルシウム材料による骨再生と血管新生の観察

    黒羽根 壮, 塩飽 由香利, 穴田 貴久, 濱井 瞭, 飯久保 正弘, 高橋 哲, 鈴木 治

    日本歯科理工学会誌　38　(Special Issue73)　10-10　2019/04

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  63. Angiogenesis involvement by octacalcium phosphate-gelatin composite-driven bone regeneration in rat calvaria critical-sized defect. International-journal Peer-reviewed

    Tsuyoshi Kurobane, Yukari Shiwaku, Takahisa Anada, Ryo Hamai, Kaori Tsuchiya, Kazuyoshi Baba, Masahiro Iikubo, Tetsu Takahashi, Osamu Suzuki

    Acta biomaterialia　88　514-526　2019/04/01

    DOI： 10.1016/j.actbio.2019.02.021 　

    ISSN： 1742-7061

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    Effect of octacalcium phosphate/gelatin composite (OCP/Gel) on angiogenesis was studied by its implantation in rat calvaria critical-sized defect in relation to bone regeneration for 2 and 4 weeks. The implantation of OCP/Gel disks was analyzed by radiomorphometry using a radiopaque material perfusion (Microfil®) method and histomorphometry by hematoxylin and eosin-staining before and after the decalcification. Effect of the OCP dose in the range up to 4 mg per well on the capillary-like tube formation by human umbilical vein endothelial cells (HUVECs) was also examined in a transwell cell culture. The results showed that the blood vessels formation by OCP/Gel group was significantly higher at 2 weeks than other groups but decreased at 4 weeks during the advancement of new bone formation. The capillary-like tube formation was highest in an OCP dose of 1 mg per well while other OCP doses above or below 1 mg did not show such a stimulatory effect. The results established both in vivo and in vitro confirmed that OCP has a positive effect on angiogenesis during bone regeneration in a suitable dose ranges, suggesting that the angiogenesis stimulated by OCP could be involved in the OCP/Gel-enhanced bone regeneration. STATEMENT OF SIGNIFICANCE: We have reported that octacalcium phosphate (OCP) materials display stimulatory capacities on the bone tissue-related cells. However, the effect of OCP on the angiogenesis and its relation to the OCP-enhanced bone regeneration is unknown. This study confirmed the capacity of OCP on angiogenesis before increasing the new bone mass after the implantation of a composite of OCP and gelatin (OCP/Gel). The blood vessels formation took place associated with the area beginning of the new bone formation, which finally decreased together with development of bone formation. Because OCP was ascertained stimulating the capillary-like tube formation in HUVEC culture with a certain OCP dose, the present study is the first report showing the capacity of OCP on angiogenesis during the OCP/Gel-enhanced bone regeneration.

  64. Culture of hybrid spheroids composed of calcium phosphate materials and mesenchymal stem cells on an oxygen-permeable culture device to predict in vivo bone forming capability. International-journal Peer-reviewed

    Tomoya Sato, Takahisa Anada, Ryo Hamai, Yukari Shiwaku, Kaori Tsuchiya, Susumu Sakai, Kazuyoshi Baba, Keiichi Sasaki, Osamu Suzuki

    Acta biomaterialia　88　477-490　2019/04/01

    DOI： 10.1016/j.actbio.2019.03.001 　

    ISSN： 1742-7061

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    Three-dimensional (3-D) cell culture can better mimic physiological conditions in which cells interact with adjacent cells and the extracellular matrix than monolayer culture. We have developed a 3-D cell culture device, the Oxy chip, which can be used to generate and supply oxygen to cell spheroids to prevent hypoxia. Here, we used the Oxy chip to generate hybrid spheroids comprising calcium phosphate (CaP) particles (hydroxyapatite (HA), β-tricalcium phosphate (β-TCP) or octacalcium phosphate (OCP)) and mesenchymal stem cells (MSCs, C3H10T1/2 cells or D1 cells) that can be used to analyze cell differentiation mechanisms. We showed that the 3-D cell-cell and cell-material interactions and oxygenation offered by the Oxy chip promoted osteoblastic differentiation of MSCs. We also used histomorphometric analysis of hematoxylin and eosin staining, quality analyses by μCT and collagen orientation observation with picrosirius red staining in bone regeneration following implantation of three CaPs in a critical-sized defect in mouse calvaria. The in vivo bone formation capacity of the three tested CaP materials was OCP ≥ β-TCP > HA: the newly formed bone by OCP had a structure relatively close to that of the calvaria intact bone. When MSCs were 3-D cultured with the CaP materials using the Oxy chip, the in vitro osteogenic capacity of these materials was highly similar to trends observed in vivo. The in vitro alkaline phosphatase activity of D1 cells had the highest correlation with in vivo bone volume (R = 0.900). Chemical and FTIR spectroscopic analyses confirmed that differentiation of D1 cells could be associated with amorphous calcium phosphate (ACP) precipitation concomitant with OCP hydrolysis. Taken together, hybrid spheroid cultures using the Oxy chip can be used to screen and predict bone forming potential of bone substitute materials. STATEMENT OF SIGNIFICANCE: An oxygen permeable-culture chip (Oxy chip) can be used to induce formation of cell spheroids by mesenchymal stem cells (MSCs). Use of the Oxy chip avoids hypoxia in the spheroid core and enhances MSC osteoblastic differentiation relative to conventional spheroid culture methods. The present study showed that the Oxy chip mimics the in vivo environment associated with bone formation and can be used to generate hybrid spheroids consisting of calcium phosphates and MSCs that are useful for analyzing cell differentiation mechanisms. Bone formation analysis following implantation of calcium phosphate materials in mouse calvaria defects showed positive correlation with the in vitro results. We propose that hybrid spheroids cultured on the Oxy chip can be used to screen and predict the bone forming potential of bone substitute materials.

  65. Vascularized Bone-Mimetic Hydrogel Constructs by 3D Bioprinting to Promote Osteogenesis and Angiogenesis. International-journal Peer-reviewed

    Takahisa Anada, Chi-Chun Pan, Alexander M Stahl, Satomi Mori, Junji Fukuda, Osamu Suzuki, Yunzhi Yang

    International journal of molecular sciences　20　(5)　2019/03/04

    DOI： 10.3390/ijms20051096 　

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    Bone is a highly vascularized tissue with a unique and complex structure. Long bone consists of a peripheral cortical shell containing a network of channels for vascular penetration and an inner highly vascularized bone marrow space. Bioprinting is a powerful tool to enable rapid and precise spatial patterning of cells and biomaterials. Here we developed a two-step digital light processing technique to fabricate a bone-mimetic 3D hydrogel construct based on octacalcium phosphate (OCP), spheroids of human umbilical vein endothelial cells (HUVEC), and gelatin methacrylate (GelMA) hydrogels. The bone-mimetic 3D hydrogel construct was designed to consist of a peripheral OCP-containing GelMA ring to mimic the cortical shell, and a central GelMA ring containing HUVEC spheroids to mimic the bone marrow space. We further demonstrate that OCP, which is evenly embedded in the GelMA, stimulates the osteoblastic differentiation of mesenchymal stem cells. We refined the design of a spheroid culture device to facilitate the rapid formation of a large number of HUVEC spheroids, which were embedded into different concentrations of GelMA hydrogels. It is shown that the concentration of GelMA modulates the extent of formation of the capillary-like structures originating from the HUVEC spheroids. This cell-loaded hydrogel-based bone construct with a biomimetic dual ring structure can be potentially used for bone tissue engineering.

  66. Octacalcium phosphate effects on the systemic and local factors that regulate bone-cell activity

    Yukari Shiwaku, Osamu Suzuki

    Octacalcium Phosphate Biomaterials: Understanding of Bioactive Properties and Application　17-36　2019/01/01

    Publisher: Elsevier

    DOI： 10.1016/B978-0-08-102511-6.00002-9 　

  67. Novel scaffold composites containing octacalcium phosphate and their role in bone repair

    Ryo Hamai, Takahisa Anada, Osamu Suzuki

    Octacalcium Phosphate Biomaterials: Understanding of Bioactive Properties and Application　121-145　2019/01/01

    Publisher: Elsevier

    DOI： 10.1016/B978-0-08-102511-6.00006-6 　

  68. 骨補填材リン酸八カルシウムの結晶性状多様性と骨形成活性 Invited Peer-reviewed

    鈴木 治, 宮武 尚央, 今泉秀樹

    日本結晶成長学会誌　46　1-10　2019

  69. リン酸八カルシウム・コラーゲン複合体に関する基礎研究から治験までの経過概要

    川井 忠, 鎌倉慎治, 松井桂子, 髙橋 哲, 鈴木 治

    日本バイオマテリアル学会誌　37　(2)　88-95　2019

    Publisher: 日本バイオマテリアル学会

    ISSN： 1347-7080

    eISSN： 2434-0359

  70. Effect of Functionally-Graded Calcium Titanate Film, Prepared by Metal-Organic Chemical Vapor Deposition, on Titanium Implant Peer-reviewed

    Shiraishi Naru, Ishiko-Uzuka Risa, Takahashi Kenta, Ogawa Toru, Anada Takahisa, Suzuki Osamu, Goto Takashi, Sasaki Keiichi

    APPLIED SCIENCES-BASEL　9　(1)　2019/01/01

    DOI： 10.3390/app9010172 　

    ISSN： 2076-3417

    eISSN： 2076-3417

  71. Surface reactivity of octacalcium phosphate-derived fluoride-containing apatite in the presence of polyols and fluoride. International-journal Peer-reviewed

    Sei Tsutsui, Takahisa Anada, Yukari Shiwaku, Kaori Tsuchiya, Hajime Yamazaki, Osamu Suzuki

    Journal of biomedical materials research. Part B, Applied biomaterials　106　(6)　2235-2244　2018/08

    DOI： 10.1002/jbm.b.34026 　

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    The present study was designed to characterize co-precipitated fluoridated apatitic materials from octacalcium phosphate (OCP) precursor and to investigate their surface reactions with polyols including glycerol in the presence of fluoride ions. Laboratory-synthesized fluoridated apatite crystals (LS-FA) were obtained in a solution containing fluoride (F) from 25 to 500 ppm. LS-FAs and commercially available fluoroapatite (FA) and hydroxyapatite (HA) were characterized by physical techniques, such as X-ray diffraction. LS-FA obtained in the presence of 100 ppmF (100 ppm-LS-FA) had an apatitic structure, but its solubility was close to HA in a culture medium (α-MEM) despite the fact it contains over 3 wt % of F. 100 ppm-LS-FA, FA, and HA were then subjected to the human serum albumin (HSA) adsorption test at pH 7.4 (in a 150 mM Tris-HCl buffer) and the dissolution and re-mineralization experiments in the presence of xylitol, D-sorbitol, or glycerol, and F under acidic and neutral conditions. Adsorption affinity of HSA was estimated as highest for FA and lowest for LS-FA. LS-FA, FA, and HA were immersed in a lactic acid solution with the polyols and/or F ion-containing solution up to 200 ppm to analyze the dissolution behavior. LS-FA had the highest dissolution tendency in the conditions examined. Glycerol enhanced the dissolution of phosphate from apatite crystals in particular from LS-FA. The results suggest that the apatite crystals, obtained through the hydrolysis of OCP in the presence of F, provide a more reactive surface than FA or HA under physiological environments. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2235-2244, 2018.

  72. 三次元細胞培養とリン酸オクタカルシウムが間葉系幹細胞の骨芽細胞分化に与える影響

    佐藤 智哉, 穴田 貴久, 塩飽 由香利, 土屋 香織, 佐々木 啓一, 鈴木 治

    日本補綴歯科学会誌　10　(特別号)　275-275　2018/06

    Publisher: (公社)日本補綴歯科学会

    ISSN： 1883-4426

    eISSN： 1883-6860

  73. リン酸オクタカルシウム・コラーゲン複合体 Invited

    川井 忠, 松井桂子, 鈴木 治, 高橋 哲, 鎌倉慎治

    歯界展望　131　(5)　870-876　2018/05

  74. Osteogenic cellular activity around onlaid octacalcium phosphate-gelatin composite onto rat calvaria Peer-reviewed

    Ryosuke Iwama, Takahisa Anada, Yukari Shiwaku, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki

    Journal of Biomedical Materials Research - Part A　106　(5)　1322-1333　2018/05/01

    Publisher: John Wiley and Sons Inc.

    DOI： 10.1002/jbm.a.36335 　

    ISSN： 1552-4965 1549-3296

    eISSN： 1552-4965

  75. Appplication of octacalcium phosphate collagen composite for dental implant treatment Invited

    17　(1)　3-8　2018/04

    Publisher:

    ISSN： 1347-894X

  76. Capacity of octacalcium phosphate to promote osteoblastic differentiation toward osteocytes in vitro Peer-reviewed

    Yuko Sai, Yukari Shiwaku, Takahisa Anada, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki

    Acta Biomaterialia　69　362-371　2018/03/15

    Publisher: Acta Materialia Inc

    DOI： 10.1016/j.actbio.2018.01.026 　

    ISSN： 1878-7568 1742-7061

    eISSN： 1878-7568

  77. Efficacy of octacalcium phosphate collagen composite for titanium dental implants in dogs Peer-reviewed

    Tadashi Kawai, Keiko Matsui, Yushi Ezoe, Fumihiko Kajii, Osamu Suzuki, Tetsu Takahashi, Shinji Kamakura

    Materials　11　(2)　pii: E229　2018/02/02

    Publisher: MDPI AG

    DOI： 10.3390/ma11020229 　

    ISSN： 1996-1944

    eISSN： 1996-1944

  78. Epogallocatechin gallate-modified gelatins with different compositions alter the quality of regenerated bone Peer-reviewed

    Harai, Y Honda, O Suzuki, T Tanaka, N Matsumoto

    Int J Mol Sci　19　pii E3232　2018

  79. Application of octacalcium phosphate and collagen composite to bone adefect in humans Peer-reviewed

    T Kawai, T Takahashi, K Matsui, Y Ezoe, O Suzuki, S Kamakura

    Clinics in Surgery　3　No.2130　2018

  80. Application of octacalcium phosphate and collagen composite for bone augmentation with sinus floor elevation in humans Peer-reviewed

    T Kawai, T Takahashi, K Matsui, Y Ezoe, F Kajii, Y Shimizu, S Koyama, H Kumamato, O Suzuki, S Kamakura

    Clinics in Surgery　3　No.2112　2018

  81. Effect of polyols on phosphorus-containing calcium fluoride deposition on hydroxyapatite surfaces Peer-reviewed

    Sei Tsutsui, Takahisa Anada, Yukari Shiwaku, Atsushi Yamagishi, Osamu Suzuki

    Journal of Hard Tissue Biology　27　(1)　34-38　2018

    Publisher: Society of Hard Tissue Regenerative Biology

    DOI： 10.2485/jhtb.27.34 　

    ISSN： 1341-7649

  82. Otimal hypoxia regulates human iPSC-derived liver bud differentiation through intercellular TGFB signaling Peer-reviewed

    H Ayabe, T Anada, T Kamoya, T satoi, M Kimurai, E Yoshizawa, S Kikuchi, Y Ueno, K Sekine, JG Camp, B Treutlein, A Ferguson, O Suzuki, T Takebe, H Taniguchi

    Stem Cell Reports　S2213-6711　30278-30279　2018

  83. Oriented bone regenerative capacity of octacalcium phosphate/gelatin composites obtained through two-step crystal preparation method Peer-reviewed

    Risa Ishiko-Uzuka, Takahisa Anada, Kazuhito Kobayashi, Tadashi Kawai, Yuji Tanuma, Keiichi Sasaki, Osamu Suzuki

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS　105　(5)　1029-1039　2017/07

    DOI： 10.1002/jbm.b.33640 　

    ISSN： 1552-4973

    eISSN： 1552-4981

  84. Octacalcium phosphate collagen composite facilitates bone regeneration of large mandibular bone defect in humans Peer-reviewed

    Tadashi Kawai, Osamu Suzuki, Keiko Matsui, Yuji Tanuma, Tetsu Takahashi, Shinji Kamakura

    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE　11　(5)　1641-1647　2017/05

    DOI： 10.1002/term.2110 　

    ISSN： 1932-6254

    eISSN： 1932-7005

  85. Application of an indicator-immobilized-gel-sheet for measuring the pH surrounding a calcium phosphate-based biomaterial Peer-reviewed

    Taisuke Masuda, Hisataka Maruyama, Fumihito Arai, Takahisa Anada, Kaori Tsuchiya, Toshio Fukuda, Osamu Suzuki

    JOURNAL OF BIOMATERIALS APPLICATIONS　31　(9)　1296-1304　2017/04

    DOI： 10.1177/0885328217699108 　

    ISSN： 0885-3282

    eISSN： 1530-8022

  86. Development of analytical methods to estimate osteoconductive activity of octacalcium phosphate materials in vitro

    Transactions of Japanese Society for Medical and Biological Engineering　55　(5)　492-492　2017

    Publisher: Japanese Society for Medical and Biological Engineering

    DOI： 10.11239/jsmbe.55Annual.492 　

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    <p>It has been advocated that bone apatite crystals are formed via precursor phases, such as octacalcium phosphate (OCP). We have developed OCP-based bone substitute materials which enhance bone formation through a mechanism that simulates bone mineralization (Jpn Dent Sci Rev 49: 58, 2013). We have designed in vitro analytical methods to estimate the bioactivity of OCP to bone tissue-related cells, such as osteoblasts. They include: 1) cell cultures on OCP-coated plate that the cells can directly attach (Biomaterials 27:267, 2006; Tissue Eng Part A 14:965, 2008); 2) cell cultures in media simulating inorganic ion composition that OCP induces at physiological pH (Dent Mater J 33:242, 2014); 3) cell culture in a 3D environment that OCP is included within the cellular construct (spheroid) (Regen Therapy 3: 58, 2016). These studies suggest that OCP stimulates the activity of bone tissue-related cells through its intrinsic physicochemical property (RSC Adv 6: 57475, 2016).</p>

  87. Effect of octacalcium phosphate-based bone substitute materials on oriented bone regeneration Peer-reviewed

    Osamu Suzuki, Takahisa Anada

    Key Engineering Materials　758　223-227　2017

    Publisher: Trans Tech Publications Ltd

    DOI： 10.4028/www.scientific.net/KEM.758.223 　

    ISSN： 1013-9826

  88. MICROFLUIDIC CHIP HAVING MULTI FLUORESCENCE MICROSENSORS FOR SPATIOTEMPORAL SENSING OF CULTURE ENVIRONMENT Peer-reviewed

    Hisataka Maruyama, Keisuke Takagi, Taisuke Masuda, Osamu Suzuki, Fumihito Arai

    30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017)　386-389　2017

    ISSN： 1084-6999

  89. Implantation of octacalcium phosphate collagen composites (OCP/Col) after extraction of canine deciduous teeth achieved undisturbed permanent tooth eruption Peer-reviewed

    Naofumi Kanda, Keiko Matsui, Tadashi Kawai, Hiroshi Edamatsu, Yuji Tanuma, Osamu Suzuki, Tetsu Takahashi, Shinji Kamakura

    ARCHIVES OF ORAL BIOLOGY　72　179-186　2016/12

    DOI： 10.1016/j.archoralbio.2016.08.027 　

    ISSN： 0003-9969

    eISSN： 1879-1506

  90. リン酸オクタカルシウム・コラーゲン複合体とインプラント体との親和性評価

    川井 忠, 松井 桂子, 江副 祐史, 梶井 文彦, 鈴木 治, 高橋 哲, 鎌倉 慎治

    Japanese Journal of Maxillo Facial Implants　15　(3)　148-148　2016/11

    Publisher: (公社)日本顎顔面インプラント学会

    ISSN： 1347-894X

  91. Effect of resorption rate and osteoconductivity of biodegradable calcium phosphate materials on the acquisition of natural bone strength in the repaired bone Peer-reviewed

    Shinpei Chiba, Takahisa Anada, Kentaro Suzuki, Keisuke Saito, Yukari Shiwaku, Naohisa Miyatake, Kazuyoshi Baba, Hideki Imaizumi, Masami Hosaka, Eiji Itoi, Osamu Suzuki

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A　104　(11)　2833-2842　2016/11

    DOI： 10.1002/jbm.a.35828 　

    ISSN： 1549-3296

    eISSN： 1552-4965

  92. An oxygen-permeable spheroid culture chip (Oxy chip) promotes osteoblastic differentiation of mesenchymal stem cells Peer-reviewed

    Takuo Kamoya, Takahisa Anada, Yukari Shiwaku, Teruko Takano-Yamamoto, Osamu Suzuki

    SENSORS AND ACTUATORS B-CHEMICAL　232　75-83　2016/09

    DOI： 10.1016/j.snb.2016.03.107 　

    ISSN： 0925-4005

  93. 上顎洞底挙上術にリン酸オクタカルシウム・コラーゲン複合体を応用し評価した1例

    川井 忠, 松井 桂子, 田中 秀典, 梶井 文彦, 岩井 敦史, 清水 良央, 熊本 裕行, 鈴木 治, 鎌倉 慎治, 高橋 哲

    日本口腔科学会雑誌　65　(2)　225-225　2016/07

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  94. Comparative study on the resorbability and dissolution behavior of octacalcium phosphate, beta-tricalcium phosphate, and hydroxyapatite under physiological conditions Peer-reviewed

    Susumu Sakai, Takahisa Anada, Kaori Tsuchiya, Hajime Yamazaki, Henry C. Margolis, Osamu Suzuki

    DENTAL MATERIALS JOURNAL　35　(2)　216-224　2016/04

    DOI： 10.4012/dmj.2015-255 　

    ISSN： 0287-4547

  95. Development of a uniaxial mechanical loading device to evaluate osteogenic differentiation of stem cells seeded on a scaffold material Peer-reviewed

    Masakazu Yamada, Takahisa Anada, Taisuke Masuda, Teruko Takano-Yamamoto, Osamu Suzuki

    2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015　2016/03/21

    Publisher: Institute of Electrical and Electronics Engineers Inc.

    DOI： 10.1109/MHS.2015.7438233 　

  96. Evaluation of bioactivity of octacalcium phosphate using osteoblastic cell aggregates on a spheroid culture device Peer-reviewed

    Takahisa Anada, Tomoya Sato, Takuo Kamoya, Yukari Shiwaku, Kaori Tsuchiya, Teruko Takano-Yamamoto, Keiichi Sasaki, Osamu Suzuki

    REGENERATIVE THERAPY　3　58-62　2016/03

    DOI： 10.1016/j.reth.2016.02.004 　

    ISSN： 2352-3204

  97. Using fluorescent polyanions to assay for osteoclastic calcium-resorption activity Peer-reviewed

    Tatsuya Miyazaki, Osamu Suzuki

    Food and Nutritional Components in Focus　2016-　(10)　111-125　2016

    Publisher: Royal Society of Chemistry

    DOI： 10.1039/9781782622130-00111 　

    ISSN： 2045-1709 2045-1695

  98. Multi Fluorescence Microsensors for Spatiotemporal Measurement of Culture Environment in a Microfluidic Chip Peer-reviewed

    Keisuke Takagi, Hisataka Maruyama, Taisuke Masuda, Osamu Suzuki, Fumihito Arai

    2016 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)　2016

    ISSN： 2474-378X

    eISSN： 2474-3798

  99. Bone matrix calcification during embryonic and postembryonic rat calvarial development assessed by SEM-EDX spectroscopy, XRD, and FTIR spectroscopy Peer-reviewed

    Akiko Henmi, Hiroshi Okata, Takahisa Anada, Mariko Yoshinari, Yasuto Mikami, Osamu Suzuki, Yasuyuki Sasano

    JOURNAL OF BONE AND MINERAL METABOLISM　34　(1)　41-50　2016/01

    DOI： 10.1007/s00774-014-0647-x 　

    ISSN： 0914-8779

    eISSN： 1435-5604

  100. Immune cell response and subsequent bone formation induced by implantation of octacalcium phosphate in a rat tibia defect Peer-reviewed

    Bunichi Hirayama, Takahisa Anada, Yukari Shiwaku, Naohisa Miyatake, Kaori Tsuchiya, Masanori Nakamura, Tetsu Takahashi, Osamu Suzuki

    RSC ADVANCES　6　(62)　S7475-S7484　2016

    DOI： 10.1039/c6ra10834b 　

    ISSN： 2046-2069

  101. A 3D culture model study monitoring differentiation of dental epithelial cells into ameloblast-like cells Peer-reviewed

    Mayu Tadaki, Takahisa Anada, Yukari Shiwaku, Takashi Nakamura, Masanori Nakamura, Masaru Kojima, Tatsuo Arai, Satoshi Fukumoto, Osamu Suzuki

    RSC ADVANCES　6　(67)　62109-62118　2016

    DOI： 10.1039/c6ra04570g 　

    ISSN： 2046-2069

  102. Dose-dependent enhancement of octacalcium phosphate biodegradation with a gelatin matrix during bone regeneration in a rabbit tibial defect model Peer-reviewed

    Keisuke Saito, Takahisa Anada, Yukari Shiwaku, Shinpei Chiba, Naohisa Miyatake, Kentaro Suzuki, Kaori Tsuchiya, Osamu Suzuki

    RSC ADVANCES　6　(69)　64165-64174　2016

    DOI： 10.1039/c6ra07602e 　

    ISSN： 2046-2069

  103. Clinical safety and efficacy of implantation of octacalcium phosphatecollagen composites in tooth extraction sockets and cyst holes Peer-reviewed

    Kawai T, Tanuma Y, Matsui K, Suzuki O, Takahashi T, Kamakura S

    J Tissue Eng　7　1-8　2016

    DOI： 10.1177/2041731416670770 　

    ISSN： 2041-7314

  104. Effect of mechanical stress on differentiation of mouse mesenchymal stem cells seeded into an octacalcium phosphate-gelatin scaffold Peer-reviewed

    Masakazu Yamada, Takahisa Anada, Taisuke Masuda, Teruko Takano-Yamamoto, Osamu Suzuki

    SENSORS AND ACTUATORS B-CHEMICAL　220　125-130　2015/12

    DOI： 10.1016/j.snb.2015.05.073 　

    ISSN： 0925-4005

  105. Orthotopic Osteogenecity Enhanced by a Porous Gelatin Sponge in a Critical-Sized Rat Calvaria Defect Peer-reviewed

    Naofumi Kanda, Takahisa Anada, Takuto Handa, Kazuhito Kobayashi, Yushi Ezoe, Tetsu Takahashi, Osamu Suzuki

    MACROMOLECULAR BIOSCIENCE　15　(12)　1647-1655　2015/12

    DOI： 10.1002/mabi.201500191 　

    ISSN： 1616-5187

    eISSN： 1616-5195

  106. Enhancement of osteoblastic differentiation in alginate gel beads with bioactive octacalcium phosphate particles Peer-reviewed

    Kosei Endo, Takahisa Anada, Masumi Yamada, Minoru Seki, Keiichi Sasaki, Osamu Suzuki

    BIOMEDICAL MATERIALS　10　(6)　065019　2015/12

    DOI： 10.1088/1748-6041/10/6/065019 　

    ISSN： 1748-6041

    eISSN： 1748-605X

  107. Synthetic octacalcium phosphate-enhanced reparative dentine formation via induction of odontoblast differentiation Peer-reviewed

    Xiaogu Wang, Tetsuo Suzawa, Tomohiko Miyauchi, Baohong Zhao, Rika Yasuhara, Takahisa Anada, Masanori Nakamura, Osamu Suzuki, Ryutaro Kamijo

    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE　9　(11)　1310-1320　2015/11

    DOI： 10.1002/term.1669 　

    ISSN： 1932-6254

    eISSN： 1932-7005

  108. Chondroitin Sulfate-E Binds to Both Osteoactivin and Integrin alpha V beta 3 and Inhibits Osteoclast Differentiation Peer-reviewed

    Tatsuya Miyazaki, Satoshi Miyauchi, Takahisa Anada, Akira Tawada, Osamu Suzuki

    JOURNAL OF CELLULAR BIOCHEMISTRY　116　(10)　2247-2257　2015/10

    DOI： 10.1002/jcb.25175 　

    ISSN： 0730-2312

    eISSN： 1097-4644

  109. リン酸オクタカルシウムの組織反応性と骨再生能

    平山 聞一, 穴田 貴久, 宮武 尚央, 土屋 香織, 中村 雅典, 高橋 哲, 鈴木 治

    日本骨代謝学会学術集会プログラム抄録集　33回　219-219　2015/07

    Publisher: (一社)日本骨代謝学会

    ISSN： 1349-0761

  110. リン酸オクタカルシウム骨再生材料の埋入早期における生体反応

    平山 聞一, 穴田 貴久, 宮武 尚央, 土屋 香織, 中村 雅典, 高橋 哲, 鈴木 治

    日本口腔科学会雑誌　64　(2)　126-126　2015/07

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

  111. The role of an octacalcium phosphate in the re-formation of infraspinatus tendon insertion Peer-reviewed

    Yoshiaki Itoigawa, Osamu Suzuki, Hirotaka Sano, Takahisa Anada, Takuto Handa, Taku Hatta, Yoshiyuki Kuwahara, Atsushi Takahashi, Yushi Ezoe, Kazuo Kaneko, Eiji Itoi

    JOURNAL OF SHOULDER AND ELBOW SURGERY　24　(7)　E175-E184　2015/07

    DOI： 10.1016/j.jse.2015.01.011 　

    ISSN： 1058-2746

  112. リン酸オクタカルシウムが関与する生体反応と骨形成の関連性

    平山 聞一, 穴田 貴久, 宮武 尚央, 土屋 香織, 中村 雅典, 高橋 哲, 鈴木 治

    Clinical Calcium　25　(5)　746-747　2015/04

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  113. Deterioration of polymethyl methacrylate dentures in the oral cavity Peer-reviewed

    Hiroshi Matsuo, Hanako Suenaga, Masatoshi Takahashi, Osamu Suzuki, Keiichi Sasaki, Nobuhiro Takahashi

    DENTAL MATERIALS JOURNAL　34　(2)　234-239　2015/04

    DOI： 10.4012/dmj.2014-089 　

    ISSN： 0287-4547

  114. Characterization of partially hydrolyzed OCP crystals deposited in a gelatin matrix as a scaffold for bone tissue engineering Peer-reviewed

    Yushi Ezoe, Takahisa Anada, Hajime Yamazaki, Takuto Handa, Kazuhito Kobayashi, Tetsu Takahashi, Osamu Suzuki

    JOURNAL OF NANOPARTICLE RESEARCH　17　(3)　127　2015/03

    DOI： 10.1007/s11051-015-2864-1 　

    ISSN： 1388-0764

    eISSN： 1572-896X

  115. Biomechanical Evaluation of Ti-Nb-Sn Alloy Implants with a Low Young's Modulus Peer-reviewed

    Kenta Takahashi, Naru Shiraishi, Risa Ishiko-Uzuka, Takahisa Anada, Osamu Suzuki, Hiroshi Masumoto, Keiichi Sasaki

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES　16　(3)　5779-5788　2015/03

    DOI： 10.3390/ijms16035779 　

    ISSN： 1422-0067

  116. SEM-CT observation of bone from mice using environmental-SEM Peer-reviewed

    Masahiro Nakajima, Masaru Takeuchi, Taisuke Masuda, Takahisa Anada, Osamu Suzuki, Toshio Fukuda, Qiang Huang, Yasuhisa Hasegawa

    2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014　2015/01/09

    Publisher: Institute of Electrical and Electronics Engineers Inc.

    DOI： 10.1109/MHS.2014.7006069 　

  117. Bone related cell-stimulating scaffold materials and a 3D cellular construct for hard tissue regeneration Peer-reviewed

    Osamu Suzuki, Takahisa Anada

    Hyper Bio Assembler for 3D Cellular Systems　261-273　2015/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-55297-0_16 　

  118. リン酸オクタカルシウム・コラーゲン複合体の埋入によるヒト抜歯寡治癒過程の評価 Peer-reviewed

    田沼裕志, 越後成志, 川井 忠, 松井桂子, 高橋 哲, 鈴木 治, 鎌倉慎治

    東北大学歯学雑誌　32　(1)　38-43　2015

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  119. Osteoconductive Property of a Mechanical Mixture of Octacalcium Phosphate and Amorphous Calcium Phosphate Peer-reviewed

    Kazuhito Kobayashi, Takahisa Anada, Takuto Handa, Naofumi Kanda, Mariko Yoshinari, Tetsu Takahashi, Osamu Suzuki

    ACS APPLIED MATERIALS & INTERFACES　6　(24)　22602-22611　2014/12

    DOI： 10.1021/am5067139 　

    ISSN： 1944-8244

  120. リン酸オクタカルシウム・コラーゲン複合体の適正規格の検討

    松井 桂子, 鎌倉 慎治, 川井 忠, 江副 祐史, 田沼 裕志, 鈴木 治, 越後 成志, 高橋 哲

    日本口腔科学会雑誌　63　(4)　446-446　2014/09

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  121. Compressive force-produced CCN2 induces osteocyte apoptosis through ERK1/2 pathway Peer-reviewed

    Kenji Hoshi, Harumi Kawaki, Ichiro Takahashi, Nobuo Takeshita, Masahiro Seiryu, Sakhr A. Murshid, Taisuke Masuda, Takahisa Anada, Ryushi Kato, Hideki Kitaura, Osamu Suzuki, Teruko Takano-Yamamoto

    Transactions of Japanese Society for Medical and Biological Engineering　52　237-O-238　2014/08/17

    Publisher: Japan Soc. of Med. Electronics and Biol. Engineering

    DOI： 10.11239/jsmbe.52.O-237 　

    ISSN： 1347-443X 1881-4379

    eISSN： 1347-443X

  122. Effect of mechanical stress on bone formation by osteoconductive octacalcium phosphate bone substitute materials Peer-reviewed

    Osamu Suzuki, Takahisa Anada, Taisuke Masuda

    Transactions of Japanese Society for Medical and Biological Engineering　52　43　2014/08/17

    Publisher: Japan Soc. of Med. Electronics and Biol. Engineering

    DOI： 10.11239/jsmbe.52.SY-43 　

    ISSN： 1347-443X 1881-4379

  123. 棘下筋腱付着部再生におけるoctacalcium phosphate(OCP)ゼラチン複合体の役割

    糸魚川 善昭, 鈴木 治, 佐野 博高, 穴田 貴久, 半田 拓人, 八田 卓久, 桑原 功行, 高橋 敦, 江副 祐史, 金子 和夫, 井樋 栄二

    日本整形外科学会雑誌　88　(8)　S1628-S1628　2014/08

    Publisher: (公社)日本整形外科学会

    ISSN： 0021-5325

  124. リン酸オクタカルシウムのラット骨欠損内における早期組織応答性

    平山 聞一, 穴田 貴久, 宮武 尚央, 小林 司史, 吉成 真理子, 中村 雅典, 高橋 哲, 鈴木 治

    日本骨代謝学会学術集会プログラム抄録集　32回　306-306　2014/07

    Publisher: (一社)日本骨代謝学会

    ISSN： 1349-0761

  125. The Regenerated Bone Quality by Implantation of Octacalcium Phosphate Collagen Composites in a Canine Alveolar Cleft Model Peer-reviewed

    Aritsune Matsui, Keiko Matsui, Takuto Handa, Yuji Tanuma, Kei-ichiro Miura, Yuta Kato, Tadashi Kawai, Osamu Suzuki, Shinji Kamakura, Seishi Echigo

    CLEFT PALATE-CRANIOFACIAL JOURNAL　51　(4)　420-430　2014/07

    DOI： 10.1597/12-096 　

    ISSN： 1545-1569

    eISSN： 1055-6656

  126. Bone response to immediate loading through titanium implants with different surface roughness in rats Peer-reviewed

    Naoko Sato, Toshie Kuwana, Miou Yamamoto, Hanako Suenaga, Takahisa Anada, Shigeto Koyama, Osamu Suzuki, Keiichi Sasaki

    ODONTOLOGY　102　(2)　249-258　2014/07

    DOI： 10.1007/s10266-013-0107-4 　

    ISSN： 1618-1247

    eISSN： 1618-1255

  127. 臨床応用に向けた、リン酸オクタカルシウム(OCP)、OCP・コラーゲン複合体(OCP/Collagen)の研究

    川井 忠, 鎌倉 愼治, 高橋 哲, 越後 成志, 鈴木 治

    東北大学歯学雑誌　33　(1)　40-40　2014/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  128. Compressive Force-Produced CCN2 Induces Osteocyte Apoptosis Through ERK1/2 Pathway Peer-reviewed

    Kenji Hoshi, Harumi Kawaki, Ichiro Takahashi, Nobuo Takeshita, Masahiro Seiryu, Sakhr A. Murshid, Taisuke Masuda, Takahisa Anada, Ryushi Kato, Hideki Kitaura, Osamu Suzuki, Teruko Takano-Yamamoto

    JOURNAL OF BONE AND MINERAL RESEARCH　29　(5)　1244-1257　2014/05

    DOI： 10.1002/jbmr.2115 　

    ISSN： 0884-0431

    eISSN： 1523-4681

  129. First Clinical Application of Octacalcium Phosphate Collagen Composite in Human Bone Defect Peer-reviewed

    Tadashi Kawai, Seishi Echigo, Keiko Matsui, Yuji Tanuma, Tetsu Takahashi, Osamu Suzuki, Shinji Kamakura

    TISSUE ENGINEERING PART A　20　(7-8)　1336-1341　2014/04

    DOI： 10.1089/ten.tea.2013.0508 　

    ISSN： 1937-3341

    eISSN： 1937-335X

  130. Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway Peer-reviewed

    Ryuhei Nishikawa, Takahisa Anada, Risa Ishiko-Uzuka, Osamu Suzuki

    DENTAL MATERIALS JOURNAL　33　(2)　242-251　2014/04

    DOI： 10.4012/dmj.2013-226 　

    ISSN： 0287-4547

  131. ゼラチン溶液中で合成したリン酸オクタカルシウムによる新規骨補填材の開発

    穴田 貴久, 石河 理紗, 佐々木 啓一, 鈴木 治

    Clinical Calcium　24　(4)　610-610　2014/03

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  132. リン酸オクタカルシウム・コラーゲン複合体を用いた骨再生療法の臨床研究

    鎌倉 慎治, 川井 忠, 田沼 裕志, 松井 桂子, 鈴木 治, 越後 成志, 高橋 哲

    Clinical Calcium　24　(4)　608-608　2014/03

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  133. The Expression of Fn14 via Mechanical Stress-activated JNK Contributes to Apoptosis Induction in Osteoblasts Peer-reviewed

    Hiroyuki Matsui, Naoto Fukuno, Yoshiaki Kanda, Yusuke Kantoh, Toko Chida, Yuko Nagaura, Osamu Suzuki, Hideki Nishitoh, Kohsuke Takeda, Hidenori Ichijo, Yasuhiro Sawada, Keiichi Sasaki, Takayasu Kobayashi, Shinri Tamura

    JOURNAL OF BIOLOGICAL CHEMISTRY　289　(10)　6438-6450　2014/03

    DOI： 10.1074/jbc.M113.536300 　

    ISSN： 0021-9258

    eISSN： 1083-351X

  134. Octacalcium phosphate: A potential scaffold material for controlling activity of bone-related cells in vitro

    Osamu Suzuki, Takahisa Anada

    Materials Science Forum　783-786　1366-1371　2014

    Publisher: Trans Tech Publications Ltd

    ISSN： 1662-9752 0255-5476

  135. リン酸オクタカルシウム・コラーゲン複合体によるヒト嚢胞摘出腔の骨再生評価

    川井 忠, 鎌倉 慎治, 松井 桂子, 田沼 裕志, 枝松 洋, 神田 直典, 小林 司史, 越後 成志, 鈴木 治, 高橋 哲

    日本口腔科学会雑誌　63　(1)　182-182　2014/01

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  136. In Vitro Assessment of Osteoblastic Differentiation of Encapsulated Stromal Cells in Alginate/Octacalcium Phosphate Peer-reviewed

    Kosei Endo, Takahisa Anada, Masumi Yamada, Minoru Seki, Keiichi Sasaki, Osamu Suzuki

    2014 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)　2014

    ISSN： 2474-378X

    eISSN： 2474-3798

  137. Effect of addition of hyaluronic acids on the osteoconductivity and biodegradability of synthetic octacalcium phosphate Peer-reviewed

    Kentaro Suzuki, Takahisa Anada, Tatsuya Miyazaki, Naohisa Miyatake, Yoshitomo Honda, Koshi N. Kishimoto, Masami Hosaka, Hideki Imaizumi, Eiji Itoi, Osamu Suzuki

    ACTA BIOMATERIALIA　10　(1)　531-543　2014/01

    DOI： 10.1016/j.actbio.2013.09.005 　

    ISSN： 1742-7061

    eISSN： 1878-7568

  138. ゼラチン担体が組織再生に与える影響についての検討

    神田 直典, 穴田 貴久, 小林 司史, 高橋 哲, 鈴木 治

    日本バイオマテリアル学会大会予稿集　35回　195-195　2013/11

    Publisher: 日本バイオマテリアル学会

  139. Characterization and bioactivity of nano-submicro octacalcium phosphate/gelatin composite Peer-reviewed

    Kei-Ichiro Miura, Takahisa Anada, Yoshitomo Honda, Yukari Shiwaku, Tadashi Kawai, Seishi Echigo, Tetsu Takahashi, Osamu Suzuki

    Applied Surface Science　282　138-145　2013/10/01

    DOI： 10.1016/j.apsusc.2013.05.086 　

    ISSN： 0169-4332

  140. Physiological Distal Drift in Rat Molars Contributes to Acellular Cementum Formation Peer-reviewed

    Shinobu Tsuchiya, Masahiro Tsuchiya, Takashi Nishioka, Osamu Suzuki, Yasuyuki Sasano, Kaoru Igarashi

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY　296　(8)　1255-1263　2013/08

    DOI： 10.1002/ar.22731 　

    ISSN： 1932-8486

    eISSN： 1932-8494

  141. Octacalcium phosphate suppresses chondrogenic differentiation of ATDC5 cells Peer-reviewed

    Isao Shibuya, Kentaro Yoshimura, Yoichi Miyamoto, Atsushi Yamada, Masamichi Takami, Tetsuo Suzawa, Dai Suzuki, Noriharu Ikumi, Fumitaka Hiura, Takahisa Anada, Osamu Suzuki, Ryutaro Kamijo

    CELL AND TISSUE RESEARCH　352　(2)　401-412　2013/05

    DOI： 10.1007/s00441-012-1548-8 　

    ISSN： 0302-766X

  142. Labile Zn ions on octacalcium phosphate-derived Zn-containing hydroxyapatite surfaces Peer-reviewed

    Yoshitomo Honda, Takahisa Anada, Shinji Morimoto, Osamu Suzuki

    APPLIED SURFACE SCIENCE　273　343-348　2013/05

    DOI： 10.1016/j.apsusc.2013.02.040 　

    ISSN： 0169-4332

    eISSN： 1873-5584

  143. Synthetic Octacalcium Phosphate Enhanced Reparative Dentin Formation via Induction of Odontoblast Differentiation Peer-reviewed

    X Wang T, Suzawa, T, Miyauchi B Zhao, R, Yasuhara T, Anada N Nakamura, O, Suzuki R Kamijo

    J Tissue Eng Regen Med　in press　2013/04

  144. リン酸オクタカルシウム・ゼラチン複合体の加水分解における結晶相の変化

    江副 祐史, 穴田 貴久, 半田 拓人, 高橋 哲, 鈴木 治

    日本歯科理工学会誌　32　(2)　82-82　2013/03

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  145. Cell encapsulation into alginate/octacalcium phosphate hydrogel beads for bone regenerative therapy Peer-reviewed

    Kosei Endo, Takahisa Anada, Masumi Yamada, Minoru Seki, Keiichi Sasaki, Osamu Suzuki

    2013 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)　2013

    ISSN： 2474-378X

    eISSN： 2474-3798

  146. Synthetic octacalcium phosphate: a possible carrier for mesenchymal stem cells in bone regeneration Peer-reviewed

    Osamu Suzuki, Takahisa Anada

    2013 35TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC)　397-400　2013

    ISSN： 1557-170X

  147. Evaluation of Hydroxyapatite Film by Powder Jet Deposition after Artificial Aging Peer-reviewed

    Ryo Akatsuka, Ken Matsumura, Miyoko Noji, Chihiro Nishikawa, Kei Sato, Takayuki Hagiwara, Takahisa Anada, Osamu Suzuki, Tunemoto Kuriyagawa, Keiichi Sasaki

    Key Engineering Materials　529-530　229-232　2013

    Publisher: Trans Tech Publications, Ltd.

    DOI： 10.4028/www.scientific.net/KEM.529-530.229 　

    eISSN： 1662-9795

    More details Close

    This study aimed to create a thick hydroxyapatite (HAp) film on the surface of a human tooth by using a newly developed powder jet deposition (PJD) device for dental handpieces, and sought to examine the microstructural and mechanical properties of the resulting HAp film. The film was evaluated on three-dimensional view, surface roughness, Vickers hardness, and bonding strength before and after artificial aging through thermal cycling (555°C) for 500 cycles (30 sec for each cycle, 20 sec of dwell time).The HAp particles in the deposited film were densely packed, and the HAp films three-dimensional microstructure and its rough surface were maintained after thermal cycling. There was no significant difference in either the HAp films Vickers hardness or the bonding strength between the film and the enamel substrate before and after thermal cycling. The HAp films created in this study demonstrated excellent microstructural and mechanical properties even after the application of thermal stress. We demonstrated the possibility of using a new type of powder jet deposition (PJD) method we developed to form a new type of interface between the tooth and biomaterials. Consequently, we propose the use of this method in new dental treatments.

  148. Cortical bone tissue response of injectable octacalcium phosphate-hyaluronic acid complexes Peer-reviewed

    Suzuki, K.a, Anada, T, Honda, Y, c, Kishimoto, K.N, Miyatake, N, Hosaka, M.a, Imaizumi, H.b e, Itoi, E.a, Suzuki, O

    Key Eng Mat　529-530　(1)　296-299　2013

    DOI： 10.4028/www.scientific.net/KEM.529-530.296 　

  149. Comparison of bone regeneration between octacalcium phosphate/collagen composite and beta-tricalcium phosphate in canine calvarial defect Peer-reviewed

    Yuji Tanuma, Keiko Matsui, Tadashi Kawai, Aritsune Matsui, Osamu Suzuki, Shinji Kamakura, Seishi Echigo

    ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY　115　(1)　9-17　2013/01

    DOI： 10.1016/j.oooo.2011.12.029 　

    ISSN： 2212-4403

    eISSN： 1528-395X

  150. Size regulation of chondrocyte spheroids using a PDMS-based cell culture chip Peer-reviewed

    Takahisa Anada, Osamu Suzuki

    Journal of Robotics and Mechatronics　25　(4)　644-649　2013

    Publisher: Fuji Technology Press

    DOI： 10.20965/jrm.2013.p0644 　

    ISSN： 1883-8049 0915-3942

  151. A microfabricated platform to form three-dimensional toroidal multicellular aggregate Peer-reviewed

    Taisuke Masuda, Natsuki Takei, Takuma Nakano, Takahisa Anada, Osamu Suzuki, Fumihito Arai

    BIOMEDICAL MICRODEVICES　14　(6)　1085-1093　2012/12

    DOI： 10.1007/s10544-012-9713-0 　

    ISSN： 1387-2176

    eISSN： 1572-8781

  152. Structural, morphological and surface characteristics of two types of octacalcium phosphate-derived fluoride-containing apatitic calcium phosphates Peer-reviewed

    Y. Shiwaku, T. Anada, H. Yamazaki, Y. Honda, S. Morimoto, K. Sasaki, O. Suzuki

    ACTA BIOMATERIALIA　8　(12)　4417-4425　2012/12

    DOI： 10.1016/j.actbio.2012.07.041 　

    ISSN： 1742-7061

  153. ヒト嚢胞摘出腔におけるリン酸オクタカルシウム・コラーゲン複合体の骨再生能

    川井 忠, 鎌倉 慎治, 松井 桂子, 田沼 裕志, 枝松 洋, 神田 直典, 小林 司史, 越後 成志, 鈴木 治, 高橋 哲

    Japanese Journal of Maxillo Facial Implants　11　(3)　160-160　2012/11

    Publisher: (公社)日本顎顔面インプラント学会

    ISSN： 1347-894X

  154. イヌ頭蓋冠臨界骨欠損におけるOCP含有率の異なるOCP/Colの骨再生能と機械的性質

    小林 司史, 松井 桂子, 川井 忠, 枝松 洋, 神田 直典, 鈴木 治, 鎌倉 慎治, 高橋 哲

    Japanese Journal of Maxillo Facial Implants　11　(3)　236-236　2012/11

    Publisher: (公社)日本顎顔面インプラント学会

    ISSN： 1347-894X

  155. リン酸オクタカルシウムコラーゲン複合体を介した骨補填材移植後の歯の萠出の解析

    神田 直典, 松井 桂子, 川井 忠, 枝松 洋, 小林 司史, 鈴木 治, 鎌倉 慎治, 越後 成志, 高橋 哲

    Japanese Journal of Maxillo Facial Implants　11　(3)　239-239　2012/11

    Publisher: (公社)日本顎顔面インプラント学会

    ISSN： 1347-894X

  156. 成犬頭蓋冠臨界骨欠損におけるOCP含有率の異なるOCP/コラーゲン複合体の骨再生能

    小林 司史, 松井 桂子, 川井 忠, 枝松 洋, 神田 直典, 鈴木 治, 鎌倉 慎治, 高橋 哲

    日本バイオマテリアル学会大会予稿集　シンポジウム2012　190-190　2012/11

    Publisher: 日本バイオマテリアル学会

  157. リン酸オクタカルシウム・コラーゲン複合体の臨床応用

    川井 忠, 鎌倉 慎治, 松井 桂子, 田沼 裕志, 小林 司史, 枝松 洋, 神田 直典, 越後 成志, 鈴木 治, 高橋 哲

    日本バイオマテリアル学会大会予稿集　シンポジウム2012　328-328　2012/11

    Publisher: 日本バイオマテリアル学会

  158. An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids Peer-reviewed

    Takahisa Anada, Junji Fukuda, Yuko Sai, Osamu Suzuki

    BIOMATERIALS　33　(33)　8430-8441　2012/11

    DOI： 10.1016/j.biomaterials.2012.08.040 　

    ISSN： 0142-9612

  159. 成犬頭蓋冠臨界骨欠損におけるOCP含有率の異なるOCP/Colの骨再生能

    小林 司史, 松井 桂子, 川井 忠, 枝松 洋, 神田 直典, 鈴木 治, 鎌倉 慎治, 越後 成志, 高橋 哲

    Journal of Oral Biosciences Supplement　2012　120-120　2012/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  160. 犬におけるリン酸オクタカルシウムコラーゲン複合体(OCP/Col)を介した歯の萠出の解析

    神田 直典, 松井 桂子, 川井 忠, 枝松 洋, 小林 司史, 鈴木 治, 鎌倉 慎治, 越後 成志, 高橋 哲

    Journal of Oral Biosciences Supplement　2012　120-120　2012/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  161. Octacalcium phosphate collagen composites with titanium mesh facilitate alveolar augmentation in canine mandibular bone defects Peer-reviewed

    K. Miura, K. Matsui, T. Kawai, Y. Kato, A. Matsui, O. Suzuki, S. Kamakura, S. Echigo

    INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY　41　(9)　1161-1169　2012/09

    DOI： 10.1016/j.ijom.2012.05.020 　

    ISSN： 0901-5027

  162. Stimulatory capacity of an octacalcium phosphate/gelatin composite on bone regeneration in rabbit tibia defect model. Peer-reviewed

    K Suzuki Y, Honda, T, Anada T, Handa, N, Miyatake A, Takahashi M Hosaka, H, Imaizumi E, Itoi O Suzuki

    Phosphorus Res Bull　26　53-58　2012/09/01

    DOI： 10.3363/prb.26.53 　

  163. Comparative study on in vitro biocompatibility of synthetic octacalcium phosphate and calcium phosphate ceramics used clinically Peer-reviewed

    Shinji Morimoto, Takahisa Anada, Yoshitomo Honda, Osamu Suzuki

    BIOMEDICAL MATERIALS　7　(4)　45020　2012/08

    DOI： 10.1088/1748-6041/7/4/045020 　

    ISSN： 1748-6041

    eISSN： 1748-605X

  164. Biomechanical Evaluation of Amorphous Calcium Phosphate Coated TNTZ Implants Prepared Using a Radiofrequency Magnetron Sputtering System Peer-reviewed

    Naru Shiraishi, Rong Tu, Risa Uzuka, Takahisa Anada, Takayuki Narushima, Takashi Goto, Mitsuo Niinomi, Keiichi Sasaki, Osamu Suzuki

    MATERIALS TRANSACTIONS　53　(7)　1343-1348　2012/07

    DOI： 10.2320/matertrans.M2012078 　

    ISSN： 1345-9678

    eISSN： 1347-5320

  165. リン酸オクタカルシウム・コラーゲン複合体(OCP/Col)を用いた臨床試験の2例

    小林 司史, 川井 忠, 神田 直典, 枝松 洋, 松井 桂子, 鈴木 治, 鎌倉 慎治, 越後 成志, 高橋 哲

    東北大学歯学雑誌　31　(1)　63-63　2012/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  166. PMMAレジンの口腔内生物学的劣化の多面的評価

    松尾 洋, 末永 華子, 高橋 正敏, 鈴木 治, 佐々木 啓一, 高橋 信博

    日本補綴歯科学会誌　4　(特別号)　97-97　2012/05

    Publisher: (公社)日本補綴歯科学会

    ISSN： 1883-4426

    eISSN： 1883-6860

  167. MSCの分化条件の検討とOCP/Colと組み合わせた担体の骨再生能の検討

    川井 忠, 穴田 貴久, 益田 泰輔, 本田 義知, 坂井 裕大, 加藤 幸夫, 鎌倉 慎治, 越後 成志, 鈴木 治

    Clinical Calcium　22　(5)　748-748　2012/04

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  168. イヌ人工的下顎骨辺縁切除モデルにおけるOCP/Colの骨再生とメカニカルストレスの影響の検討

    三浦 圭一郎, 松井 桂子, 松井 有恒, 鈴木 治, 鎌倉 慎治, 越後 成志

    Clinical Calcium　22　(5)　748-749　2012/04

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  169. The effect of an octacalcium phosphate co-precipitated gelatin composite on the repair of critical-sized rat calvarial defects Peer-reviewed

    T. Handa, T. Anada, Y. Honda, H. Yamazaki, K. Kobayashi, N. Kanda, S. Kamakura, S. Echigo, O. Suzuki

    ACTA BIOMATERIALIA　8　(3)　1190-1200　2012/03

    DOI： 10.1016/j.actbio.2011.12.002 　

    ISSN： 1742-7061

  170. Granule Size-Dependent Bone Regenerative Capacity of Octacalcium Phosphate in Collagen Matrix Peer-reviewed

    Yuji Tanuma, Takahisa Anada, Yoshitomo Honda, Tadashi Kawai, Shinji Kamakura, Seishi Echigo, Osamu Suzuki

    TISSUE ENGINEERING PART A　18　(5-6)　546-557　2012/03

    DOI： 10.1089/ten.tea.2011.0349 　

    ISSN： 1937-3341

    eISSN： 1937-335X

  171. Hydroxyapatite film for dental treatment by powder jet deposition: A review Peer-reviewed

    Ryo Akatsuka, Hiroshi Ishihata, Miyoko Noji, Ken Matsumura, Takahiro Anada, Tunemoto Kuriyagawa, Osamu Suzuki, Keiichi Sasaki

    Interface Oral Health Science 2011　246-248　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0-70 　

  172. Highly biodegradable bone substitute materials with OCP Peer-reviewed

    Osamu Suzuki, Takahisa Anada

    Interface Oral Health Science 2011　321-326　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_96 　

  173. Signal Pathway Analysis with differentiation markers in osteoblasts stimulated by synthetic analogue to bone mineral Peer-reviewed

    Ryuhei Nishikawa, Takahisa Anada, Osamu Suzuki

    2012 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)　164-168　2012

    ISSN： 2474-378X

    eISSN： 2474-3798

  174. Erratum: Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals (Analytical Biochemistry (2011) 418 (276-285)) Peer-reviewed

    Kaneko H, Kamiie J, Kawakami H, Anada T, Honda Y, Shiraishi N, Kamakura S, Terasaki T, Shimauchi H, Suzuki O

    Analytical Biochemistry　420　(2)　198-198　2012

    DOI： 10.1016/j.ab.2011.09.023 　

    ISSN： 0003-2697

  175. Calcium phosphate-coated titanium alloy implants prepared by radiofrequency magnetron sputtering: a review Peer-reviewed

    N. Shiraishi, Y. Suzuki, N. Sato, T. Anada, T. Goto, R. Tu, M. Niinomi, T. Narushima, K. Ueda, R. Uzuka, O. Suzuki, K. Sasaki

    Interface Oral Health Science 2011　352-354　2012

    DOI： 10.1007/978-4-431-54070-0_102 　

  176. Changes in stress distribution of orthodontic miniscrews and surrounding bone evaluated by 3-dimensional finite element analysis Peer-reviewed

    Akihiro Suzuki, Taisuke Masuda, Ichiro Takahashi, Toru Deguchi, Osamu Suzuki, Teruko Takano-Yamamoto

    AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS　140　(6)　E273-E280　2011/12

    DOI： 10.1016/j.ajodo.2011.06.025 　

    ISSN： 0889-5406

  177. リン酸オクタカルシウム/ゼラチン複合体(OCP/Gel)によるラット頭蓋冠臨界径骨欠損の骨再生

    半田 拓人, 本田 義知, 穴田 貴久, 鎌倉 慎治, 越後 成志, 鈴木 治

    日本バイオマテリアル学会大会予稿集　33回　280-280　2011/11

    Publisher: 日本バイオマテリアル学会

  178. 骨再生材料ナノOCP/ゼラチン複合体の合成と機能評価

    三浦 桂一郎, 穴田 貴久, 本田 義知, 鎌倉 慎治, 越後 成志, 鈴木 治

    日本バイオマテリアル学会大会予稿集　33回　129-129　2011/11

    Publisher: 日本バイオマテリアル学会

  179. Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals Peer-reviewed

    Hirofumi Kaneko, Junichi Kamiie, Hirotaka Kawakami, Takahisa Anada, Yoshitomo Honda, Naru Shiraishi, Shinji Kamakura, Tetsuya Terasaki, Hidetoshi Shimauchi, Osamu Suzuki

    ANALYTICAL BIOCHEMISTRY　418　(2)　276-285　2011/11

    DOI： 10.1016/j.ab.2011.07.022 　

    ISSN： 0003-2697

    eISSN： 1096-0309

  180. OCP/Col複合体のOCP顆粒径の違いによる骨再生能の比較および骨質評価

    加藤 雄大, 松井 桂子, 川井 忠, 田沼 裕志, 松井 有恒, 鈴木 治, 鎌倉 慎治, 越後 成志

    日本口腔外科学会雑誌　57　(Suppl.)　121-121　2011/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  181. イヌ下顎骨辺縁切除モデルでのOCP/Colの骨造成能とメカニカルストレスの影響の検討

    三浦 桂一郎, 松井 桂子, 松井 有恒, 鈴木 治, 鎌倉 慎治, 越後 成志

    日本口腔外科学会雑誌　57　(Suppl.)　207-207　2011/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  182. 未萠出永久歯のある顎裂閉鎖に応用可能な骨再生材料としてのOCP/Colの検討

    松井 桂子, 神田 直典, 三浦 桂一郎, 枝松 洋, 小林 司史, 加藤 雄大, 半田 拓人, 田沼 裕志, 鈴木 治, 鎌倉 慎治, 越後 成志

    日本口腔外科学会雑誌　57　(Suppl.)　290-290　2011/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  183. 骨再生材料OCP/Colによる再生骨の解析(犬人工的顎裂モデルへの応用)

    松井 有恒, 松井 桂子, 田沼 裕志, 半田 拓人, 加藤 雄大, 三浦 桂一郎, 鈴木 治, 鎌倉 慎治, 越後 成志

    日本口腔外科学会雑誌　57　(Suppl.)　291-291　2011/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  184. Characteristics of hydroxyapatite film formed on human enamel with the powder jet deposition technique Peer-reviewed

    Ryo Akatsuka, Keiichi Sasaki, Mohammed Saeed Sepasy Zahmaty, Miyoko Noji, Takahisa Anada, Osamu Suzuki, Tsunemoto Kuriyagawa

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS　98B　(2)　210-216　2011/08

    DOI： 10.1002/jbm.b.31781 　

    ISSN： 1552-4973

  185. PMMAレジンの口腔環境における生物学的劣化(biodeterioration) 使用義歯の劣化の評価について

    松尾 洋, 末永 華子, 高橋 正敏, 鈴木 治, 佐々木 啓一, 高橋 信博

    日本補綴歯科学会誌　3　(特別号)　287-287　2011/05

    Publisher: (公社)日本補綴歯科学会

    ISSN： 1883-4426

    eISSN： 1883-6860

  186. TGF-beta-activated kinase 1 mediates mechanical stress-induced IL-6 expression in osteoblasts Peer-reviewed

    Naoto Fukuno, Hiroyuki Matsui, Yoshiaki Kanda, Osamu Suzuki, Kunihiro Matsumoto, Keiichi Sasaki, Takayasu Kobayashi, Shinri Tamura

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　408　(2)　202-207　2011/05

    DOI： 10.1016/j.bbrc.2011.02.127 　

    ISSN： 0006-291X

  187. Effect of Zn2+ on the Physicochemical Characteristics of Octacalcium Phosphate and Its Hydrolysis into Apatitic Phases Peer-reviewed

    Yoshitomo Honda, Takahisa Anada, Shinji Morimoto, Yukari Shiwaku, Osamu Suzuki

    CRYSTAL GROWTH & DESIGN　11　(5)　1462-1468　2011/05

    DOI： 10.1021/cg1009835 　

    ISSN： 1528-7483

    eISSN： 1528-7505

  188. 生体内吸収性を示すリン酸オクタカルシウム(OCP)/ゼラチン骨再生材料の開発

    半田 拓人, 本田 義知, 穴田 貴久, 益田 泰輔, 鎌倉 慎治, 越後 成志, 鈴木 治

    Clinical Calcium　21　(5)　758-759　2011/04

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  189. リン酸オクタカルシウム・コラーゲン複合体(OCP/Col)を応用したイヌ人工的顎裂モデルの骨再生

    松井 桂子, 松井 有恒, 半田 拓人, 川井 忠, 鈴木 治, 鎌倉 慎治, 越後 成志

    Clinical Calcium　21　(5)　759-759　2011/04

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  190. Enhancement of Bone Morphogenetic Protein-2-Induced Ectopic Bone Formation by Transforming Growth Factor-beta 1 Peer-reviewed

    Keita Tachi, Masamichi Takami, Hana Sato, Ayako Mochizuki, Baohong Zhao, Yoichi Miyamoto, Hiroaki Tsukasaki, Tomio Inoue, Satoru Shintani, Tatsuya Koike, Yoshitomo Honda, Osamu Suzuki, Kazuyoshi Baba, Ryutaro Kamijo

    TISSUE ENGINEERING PART A　17　(5-6)　597-606　2011/03

    DOI： 10.1089/ten.tea.2010.0094 　

    ISSN： 1937-3341

  191. Evaluation of osteoclastic resorption activity using calcium phosphate coating combined with labeled polyanion Peer-reviewed

    Tatsuya Miyazaki, Satoshi Miyauchi, Takahisa Anada, Hideki Imaizumi, Osamu Suzuki

    ANALYTICAL BIOCHEMISTRY　410　(1)　7-12　2011/03

    DOI： 10.1016/j.ab.2010.11.014 　

    ISSN： 0003-2697

  192. Fibroblast growth factor 10 regulates Meckel&apos;s cartilage formation during early mandibular morphogenesis in rats Peer-reviewed

    Fumie Terao, Ichiro Takahashi, Hidetoshi Mitani, Naoto Haruyama, Yasuyuki Sasano, Osamu Suzuki, Teruko Takano-Yamamoto

    DEVELOPMENTAL BIOLOGY　350　(2)　337-347　2011/02

    DOI： 10.1016/j.ydbio.2010.11.029 　

    ISSN： 0012-1606

  193. MSCを播種したOCP/Collagen複合体による骨再生能の長期的検討

    川井 忠, 穴田 貴久, 益田 泰輔, 本田 義知, 坂井 裕大, 加藤 幸夫, 鎌倉 慎治, 越後 成志, 鈴木 治

    日本口腔科学会雑誌　60　(1)　131-131　2011/01

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  194. イヌ人工的顎裂部における骨補填材OCP/Colの骨再生能と再生骨の骨質評価

    松井 有恒, 松井 桂子, 君塚 哲, 田沼 裕志, 半田 拓人, 鈴木 治, 鎌倉 慎治, 越後 成志

    日本口腔科学会雑誌　60　(1)　131-132　2011/01

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  195. Identification of serum proteins accumulated onto octacalcium phosphate in vitro Peer-reviewed

    Kaneko H, Kamiie J, Kawakami H, Anada T, Honda Y, Kamakura S, Terasaki T, Shimauchi H, Suzuki O

    24th European Conference on Biomaterials - Annual Conference of the European Society for Biomaterials　2011

  196. Reconstruction of Critical-Sized Bone Defect in Dog Skull by Octacalcium Phosphate Combined with Collagen Peer-reviewed

    Tadashi Kawai, Keiko Matsui, Shinya Iibuchi, Takahisa Anada, Yoshitomo Honda, Kazuo Sasaki, Shinji Kamakura, Osamu Suzuki, Seishi Echigo

    CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH　13　(2)　112-123　2011

    DOI： 10.1111/j.1708-8208.2009.00192.x 　

    ISSN： 1523-0899

    eISSN： 1708-8208

  197. The effect of synthetic octacalcium phosphate in a collagen scaffold on the osteogenicity of mesenchymal stem cells Peer-reviewed

    Tadashi Kawai, Takahisa Anada, Taisuke Masuda, Yoshitomo Honda, Yuhiro Sakai, Yukio Kato, Shinji Kamakura, Seishi Echigo, Osamu Suzuki

    European Cells and Materials　22　124-136　2011

    Publisher: AO Research Institute Davos

    DOI： 10.22203/eCM.v022a10 　

    ISSN： 1473-2262

  198. Bone regeneration by octacalcium phosphate collagen composites in a dog alveolar cleft model Peer-reviewed

    K. Matsui, A. Matsui, T. Handa, T. Kawai, O. Suzuki, S. Kamakura, S. Echigo

    INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY　39　(12)　1218-1225　2010/12

    DOI： 10.1016/j.ijom.2010.07.015 　

    ISSN： 0901-5027

  199. リン酸オクタカルシウム・コラーゲン複合体のOCP顆粒径の違いによる骨形成能の比較

    加藤 雄大, 松井 桂子, 川井 忠, 田沼 裕志, 三浦 桂一郎, 半田 拓人, 松井 有恒, 鈴木 治, 鎌倉 慎治, 越後 成志

    日本口腔外科学会雑誌　56　(Suppl.)　204-204　2010/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  200. Octacalcium phosphate: Osteoconductivity and crystal chemistry Invited Peer-reviewed

    O. Suzuki

    ACTA BIOMATERIALIA　6　(9)　3379-3387　2010/09

    DOI： 10.1016/j.actbio.2010.04.002 　

    ISSN： 1742-7061

  201. Effect of chondroitin sulfate-E on the osteoclastic differentiation of RAW264 cells Peer-reviewed

    Tatsuya Miyazaki, Satoshi Miyauchi, Akira Tawada, Takahisa Anada, Osamu Suzuki

    DENTAL MATERIALS JOURNAL　29　(4)　403-410　2010/07

    DOI： 10.4012/dmj.2009-132 　

    ISSN： 0287-4547

  202. Quality of Regenerated Bone Enhanced by Implantation of Octacalcium Phosphate-Collagen Composite Peer-reviewed

    Taisuke Masuda, Tadashi Kawai, Takahisa Anada, Shinji Kamakura, Osamu Suzuki

    TISSUE ENGINEERING PART C-METHODS　16　(3)　471-478　2010/06

    DOI： 10.1089/ten.tec.2009.0212 　

    ISSN： 1937-3384

  203. Analysis of physicochemical properties of octacalcium phosphate prepared by hydrolysis and co-precipitation with fluoride ions Peer-reviewed

    Yukari Shiwaku, Yoshitomo Honda, Takahisa Anada, Shinji Morimoto, Taisuke Masuda, Keiichi Sasaki, Osamu Suzuki

    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN　118　(1378)　402-405　2010/06

    DOI： 10.2109/jcersj2.118.402 　

    ISSN： 1882-0743

    eISSN： 1348-6535

  204. Three-dimensional cell culture device utilizing thin membrane deformation by decompression Peer-reviewed

    Takahisa Anada, Taisuke Masuda, Yoshitomo Honda, Junji Fukuda, Fumihito Arai, Toshio Fukuda, Osamu Suzuki

    SENSORS AND ACTUATORS B-CHEMICAL　147　(1)　376-379　2010/05

    DOI： 10.1016/j.snb.2010.01.065 　

    ISSN： 0925-4005

  205. Enhancement of octacalcium phosphate deposition on a titanium surface activated by electron cyclotron resonance plasma oxidation Peer-reviewed

    Yusuke Orii, Hiroshi Masumoto, Yoshitomo Honda, Takahisa Anada, Takashi Goto, Keiichi Sasaki, Osamu Suzuki

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS　93B　(2)　476-483　2010/05

    DOI： 10.1002/jbm.b.31605 　

    ISSN： 1552-4973

  206. 早期分化誘導MSCを播種したOCP/Col複合体の骨再生能

    川井 忠, 穴田 貴久, 益田 泰輔, 本田 義知, 坂井 裕大, 加藤 幸夫, 鎌倉 慎治, 越後 成志, 鈴木 治

    Clinical Calcium　20　(5)　788-788　2010/04

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  207. Comparative study on bone regeneration by synthetic octacalcium phosphate with various granule sizes Peer-reviewed

    Yoshihisa Murakami, Yoshitomo Honda, Takahisa Anada, Hidetoshi Shimauchi, Osamu Suzuki

    ACTA BIOMATERIALIA　6　(4)　1542-1548　2010/04

    DOI： 10.1016/j.actbio.2009.10.023 　

    ISSN： 1742-7061

  208. OCPコラーゲン複合体の骨再生能の検討 OCP顆粒径と架橋温度の影響

    田沼 裕志, 穴田 貴久, 益田 泰輔, 本田 義知, 松井 有恒, 川井 忠, 鎌倉 慎治, 越後 成志, 鈴木 治

    日本歯科理工学会誌　29　(2)　119-119　2010/03

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  209. 骨再生材料としてのOCP析出ゼラチン担体の開発

    半田 拓人, 本田 義知, 穴田 貴久, 益田 泰輔, 窪田 弘幸, 鎌倉 慎治, 越後 成志, 鈴木 治

    日本歯科理工学会誌　29　(2)　156-156　2010/03

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  210. Preparation and characterization of porous alginate scaffolds containing various amounts of octacalcium phosphate (OCP) crystals Invited Peer-reviewed

    Naru Shiraishi, Takahisa Anada, Yoshitomo Honda, Taisuke Masuda, Keiichi Sasaki, Osamu Suzuki

    JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE　21　(3)　907-914　2010/03

    DOI： 10.1007/s10856-009-3911-1 　

    ISSN： 0957-4530

  211. TGF-β1はBMP-2による骨形成を強力に促進する

    舘 慶太, 高見 正道, 佐藤 華, 本田 義知, 新谷 悟, 鈴木 治, 馬場 一美, 上條 竜太郎

    口腔組織培養学会誌　19　(1)　27-28　2010/02

    Publisher: 日本口腔組織培養学会

    ISSN： 1347-6661

  212. Octacalcium phosphate (OCP) collagen composites enhance bone healing in a dog tooth extraction socket model Peer-reviewed

    S. Iibuchi, K. Matsui, T. Kawai, K. Sasaki, O. Suzuki, S. Kamakura, S. Echigo

    INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY　39　(2)　161-168　2010/02

    DOI： 10.1016/j.ijom.2009.12.006 　

    ISSN： 0901-5027

  213. Bone regeneration by octacalcium phosphate and collagen composites

    Shinji Kamakura, Takahisa Anada, Osamu Suzuki

    Bone Regeneration: Growth Factors, Augmentation Procedures and Tissue Engineering Applications　177-202　2010/01/01

  214. S0201-1-5 Solution-mediated dissolution and cellular biodegradation of octacalcium phosphate (OCP) bone substitute material

    SUZUKI Osamu, ANADA Takahisa

    The proceedings of the JSME annual meeting　2010　19-20　2010

    Publisher: The Japan Society of Mechanical Engineers

    DOI： 10.1299/jsmemecjo.2010.5.0_19 　

    More details Close

    Synthetic octacalcium phosphate (OCP) exhibits biodegradable characteristics if implanted in various bone defects. OCP tends to convert to hydroxyapatite (HA) both in vitro at physiologic pH and in the implanted sites. This conversion is accompanied by calcium ion consumption from and phosphate ion release into the surrounding solution. When OCP is incubated in culture medium, this tendency is more distinct as the dose of OCP increased. The equilibrium is attained to approach to the saturation with respect to OCP but slightly to higher degree of supersaturation beyond OCP. The growth of mouse bone marrow stromal ST-2 cells on OCP crystals is inhibited in the initial stage of incubation while osteoblastic differentiation is facilitated at the later stage more than that by HA crystals. The slight advancement of OCP hydrolysis into HA inhibits the appearance of osteoclastic cells around the OCP-implant in rat tibia bone marrow spaces. The results suggests that the characteristics of OCP which tend to convert to HA is a factor that stimulates not only osteoblastic cellular activity but also osteoclastic cellular response induced by OCP crystals.

  215. Octacalcium phosphate-precipitated gelatin scaffold as a bone substitute material

    Handa Takuto, Honda Yoshitomo, Anada Takahisa, Masuda Taisuke, Kubota Hiroyuki, Kamakura Sinji, Echigo Seishi, Suzuki Osamu

    Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices　2010　(0)　70-70　2010

    Publisher: The Japanese Society for Dental Materials and Devices

    DOI： 10.14832/gsjsdmd.2010s.0.70.0 　

  216. Effect of topography of the octacalcium phosphate granule surfaces on its bone regenerative property Peer-reviewed

    Yoshitomo Honda, Takahisa Anada, Shinji Kamakura, Osamu Suzuki

    INTERFACE ORAL HEALTH SCIENCE 2009　288-+　2010

    DOI： 10.1007/978-4-431-99644-6_82 　

  217. Apatite formation from octacalcium phosphate with fluoride Peer-reviewed

    Yukari Shiwaku, Yoshitomo Honda, Takahisa Anada, Keiichi Sasaki, Osamu Suzuki

    INTERFACE ORAL HEALTH SCIENCE 2009　286-+　2010

    DOI： 10.1007/978-4-431-99644-6_81 　

  218. Alginate/octacalcium phosphate composites enhance bone formation in critical-sized mouse calvaria defects Peer-reviewed

    Takeshi Fuji, Takahisa Anada, Yoshitomo Honda, Yukari Shiwaku, Keiichi Sasaki, Osamu Suzuki

    INTERFACE ORAL HEALTH SCIENCE 2009　308-+　2010

    DOI： 10.1007/978-4-431-99644-6_89 　

  219. Formation of hydroxyapatite film on tooth using powder-jet-deposition Peer-reviewed

    Ryo Akatsuka, Mohamamd Saeed Sepasy Zahmaty, Miyoko Noji, Takahisa Anada, Tsunemoto Kuriyagawa, Osamu Suzuki, Keiichi Sasaki

    INTERFACE ORAL HEALTH SCIENCE 2009　302-+　2010

    DOI： 10.1007/978-4-431-99644-6_87 　

  220. Mechanical stress modulates bone remodeling signals Peer-reviewed

    Hiroyuki Matsui, Naoto Fukuno, Osamu Suzuki, Kohsuke Takeda, Hidenori Ichijo, Takayasu Kobayashi, Shinri Tamura, Keiichi Sasaki

    INTERFACE ORAL HEALTH SCIENCE 2009　129-+　2010

    DOI： 10.1007/978-4-431-99644-6_21 　

  221. Mechanical Stress-Related Calvaria Bone Augmentation by Onlayed Octacalcium Phosphate-Collagen Implant Peer-reviewed

    Aritsune Matsui, Takahisa Anada, Taisuke Masuda, Yoshitomo Honda, Naohisa Miyatake, Tadashi Kawai, Shinji Kamakura, Seishi Echigo, Osamu Suzuki

    TISSUE ENGINEERING PART A　16　(1)　139-151　2010/01

    DOI： 10.1089/ten.tea.2009.0284 　

    ISSN： 1937-3341

    eISSN： 1937-335X

  222. Appositional Bone Formation by OCP-Collagen Composite Peer-reviewed

    Y. Suzuki, S. Kamakura, Y. Honda, T. Anada, K. Hatori, K. Sasaki, O. Suzuki

    JOURNAL OF DENTAL RESEARCH　88　(12)　1107-1112　2009/12

    DOI： 10.1177/0022034509351378 　

    ISSN： 0022-0345

  223. Osteoclast Differentiation Induced by Synthetic Octacalcium Phosphate Through Receptor Activator of NF-kappa B Ligand Expression in Osteoblasts Peer-reviewed

    Masamichi Takami, Ayako Mochizuki, Atsushi Yamada, Keita Tachi, Baohong Zhao, Yoichi Miyamoto, Takahisa Anada, Yoshitomo Honda, Tomio Inoue, Masanori Nakamura, Osamu Suzuki, Ryutaro Kamijo

    TISSUE ENGINEERING PART A　15　(12)　3991-4000　2009/12

    DOI： 10.1089/ten.tea.2009.0065 　

    ISSN： 1937-3341

    eISSN： 1937-335X

  224. Octacalcium Phosphate-Precipitated Alginate Scaffold for Bone Regeneration Peer-reviewed

    Takeshi Fuji, Takahisa Anada, Yoshitomo Honda, Yukari Shiwaku, Hiroko Koike, Shinji Kamakura, Keiichi Sasaki, Osamu Suzuki

    TISSUE ENGINEERING PART A　15　(11)　3525-3535　2009/11

    DOI： 10.1089/ten.tea.2009.0048 　

    ISSN： 1937-3341

  225. 分化培地で前培養した間葉系幹細胞のOCP/Col骨補填材への播種効果

    川井 忠, 穴田 貴久, 本田 義知, 鎌倉 慎治, 越後 成志, 鈴木 治

    日本口腔外科学会雑誌　55　(Suppl.)　169-169　2009/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  226. 成犬頭蓋骨欠損モデルにおけるOCPコラーゲン複合体とβ-TCPの骨形成能

    田沼 裕志, 松井 桂子, 川井 忠, 松井 有恒, 飯淵 信也, 佐々木 和夫, 鈴木 治, 鎌倉 慎治, 越後 成志

    日本口腔科学会雑誌　58　(4)　222-222　2009/09

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  227. リン酸オクタカルシウム・コラーゲン複合体(OCP/Col)の骨造成能とメカニカルストレスの影響

    松井 有恒, 穴田 貴久, 益田 泰輔, 本田 義知, 川井 忠, 鎌倉 慎治, 鈴木 治, 越後 成志

    日本口腔科学会雑誌　58　(4)　276-276　2009/09

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  228. 間葉系幹細胞を組み合わせたリン酸オクタカルシウム・コラーゲン複合体の骨再生能

    川井 忠, 穴田 貴久, 益田 泰輔, 本田 義知, 坂井 裕大, 加藤 幸夫, 鎌倉 慎治, 越後 成志, 鈴木 治

    日本口腔科学会雑誌　58　(4)　277-277　2009/09

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  229. Synthesis of calcium phosphate-binding liposome for drug delivery Peer-reviewed

    Takahisa Anada, Yoichi Takeda, Yoshitomo Honda, Kazuo Sakurai, Osamu Suzuki

    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　19　(15)　4148-4150　2009/08

    DOI： 10.1016/j.bmcl.2009.05.117 　

    ISSN： 0960-894X

  230. The Effect of Microstructure of Octacalcium Phosphate on the Bone Regenerative Property Peer-reviewed

    Yoshitomo Honda, Takahisa Anada, Shinji Kamakura, Shinji Morimoto, Tsunemoto Kuriyagawa, Osamu Suzuki

    TISSUE ENGINEERING PART A　15　(8)　1965-1973　2009/08

    DOI： 10.1089/ten.tea.2008.0300 　

    ISSN： 1937-3341

    eISSN： 1937-335X

  231. 骨再生を探る 臨床と基礎研究

    越後 成志, 松井 桂子, 君塚 哲, 川井 忠, 松井 有恒, 飯淵 信也, 鎌倉 慎治, 鈴木 治

    東北大学歯学雑誌　28　(1)　28-28　2009/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  232. Intramembranous bone tissue response to biodegradable octacalcium phosphate implant Peer-reviewed

    T. Kikawa, O. Kashimoto, H. Imaizumi, S. Kokubun, O. Suzuki

    ACTA BIOMATERIALIA　5　(5)　1756-1766　2009/06

    DOI： 10.1016/j.actbio.2008.12.008 　

    ISSN： 1742-7061

  233. 生体吸収性OCP/Collagen複合体の骨造成能とメカニカルストレスの影響

    松井 有恒, 穴田 貴久, 益田 泰輔, 本田 義知, 川井 忠, 鎌倉 慎治, 越後 成志, 鈴木 治

    Clinical Calcium　19　(6)　858-858　2009/05

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  234. Characteristics of the hydroxyapatite film deposited on human enamel:deposition of a ceramic film by powder jet deposition technique Peer-reviewed

    Miyoko Noji, Mohammad Saeed, Sepasy Zahmaty, Toshihiko Shibuya, Nobuhito Yoshihara, Tsunemoto Kuriyagawa, Keiichi Sasaki, Osamu Suzuki

    International Journal of Abrasive Technology (IJAT)　2　(1)　83-96　2009/02

    DOI： 10.1504/IJAT.2009.021640 　

  235. Effect of partial hydrolysis of octacalcium phosphate on its osteoconductive characteristics Peer-reviewed

    Naohisa Miyatake, Koshi N. Kishimoto, Takahisa Anada, Hideki Imaizumi, Eiji Itoi, Osamu Suzuki

    BIOMATERIALS　30　(6)　1005-1014　2009/02

    DOI： 10.1016/j.biomaterials.2008.10.058 　

    ISSN： 0142-9612

  236. Proteome analysis of rat serum proteins accumulated onto octacalcium phosphate (OCP) crystals

    Kaneko Hirofumi, Kamiie Junichi, Anada Takahisa, Honda Yoshitomo, Kamakura Shinji, Terasaki Tetsuya, Shimauchi Hidetoshi, Suzuki Osamu

    Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices　2009　(0)　98-98　2009

    Publisher: The Japanese Society for Dental Materials and Devices

    DOI： 10.14832/gsjsdmd.2009f.0.98.0 　

  237. Development of a pH indicator immobilized-gel-sheet for microenviroment analysis Peer-reviewed

    Taisuke Masuda, Hisataka Maruyama, Fumihito Arai, Takahisa Anada, Toshio Fukuda, Osamu Suzuki

    20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science　362-367　2009

    DOI： 10.1109/MHS.2009.5351885 　

  238. Mechanical stress induces the activation of TAK1 and its downstream pathways in pre-osteoblastic cells Peer-reviewed

    Naoto Fukuno, Hiroyuki Matsui, Osamu Suzuki, Keiichi Sasaki, Takayasu Kobayashi, Shinri Tamura

    20th Anniversary MHS 2009 and Micro-Nano Global COE - 2009 International Symposium on Micro-NanoMechatronics and Human Science　383-384　2009

    DOI： 10.1109/MHS.2009.5351995 　

  239. Synthetic Octacalcium Phosphate Augments Bone Regeneration Correlated with Its Content in Collagen Scaffold Peer-reviewed

    Tadashi Kawai, Takahisa Anada, Yoshitomo Honda, Shinji Kamakura, Keiko Matsui, Aritsune Matsui, Kazuo Sasaki, Shinji Morimoto, Seishi Echigo, Osamu Suzuki

    TISSUE ENGINEERING PART A　15　(1)　23-32　2009/01

    DOI： 10.1089/ten.tea.2008.0141 　

    ISSN： 1937-3341

    eISSN： 1937-335X

  240. Oversulfated Chondroitin Sulfate-E Binds to BMP-4 and Enhances Osteoblast Differentiation Peer-reviewed

    Tatsuya Miyazaki, Satoshi Miyauchi, Akira Tawada, Takahisa Anada, Satoshi Matsuzaka, Osamu Suzuki

    JOURNAL OF CELLULAR PHYSIOLOGY　217　(3)　769-777　2008/12

    DOI： 10.1002/jcp.21557 　

    ISSN： 0021-9541

  241. ラット頭蓋冠上骨膜下に埋入された骨置換性リン酸オクタカルシウム/Collagen複合体の骨造成反応

    松井 有恒, 穴田 貴久, 益田 泰輔, 本田 義知, 川井 忠, 松井 桂子, 越後 成志, 鎌倉 慎治, 鈴木 治

    日本骨代謝学会学術集会プログラム抄録集　26回　228-228　2008/10

    Publisher: (一社)日本骨代謝学会

    ISSN： 1349-0761

  242. OCP/アルギン酸複合体を用いた骨再生 Peer-reviewed

    冨士岳志, 穴田貴久, 本田義知, 塩飽由香利, 佐々木啓一, 鈴木治

    Journal of Oral Biosciences　50　(Supplement)　124-124　2008/09/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

  243. リン酸オクタカルシウム・コラーゲン複合体(OCP/Col)を用いたイヌ頭蓋骨臨界骨欠損部での骨再生研究

    川井 忠, 松井 桂子, 佐々木 信也, 佐々木 和夫, 鎌倉 慎治, 鈴木 治, 越後 成志

    日本口腔科学会雑誌　57　(4)　461-461　2008/09

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  244. リン酸オクタカルシウム・コラーゲン複合体(OCP/Col)埋入による骨再生能の検討

    飯淵 信也, 川井 忠, 松井 桂子, 鎌倉 慎治, 鈴木 治, 越後 成志

    日本口腔科学会雑誌　57　(4)　536-536　2008/09

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  245. リン酸オクタカルシウム(OCP)/コラーゲン複合体はOCPの用量依存的に骨再生を促進する

    川井 忠, 穴田 貴久, 本田 義知, 鎌倉 慎治, 松井 有恒, 松井 桂子, 越後 成志, 鈴木 治

    Journal of Oral Biosciences　50　(Suppl.)　112-112　2008/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  246. リン酸オクタカルシウム・コラーゲン複合体による骨再生の長期観察

    鎌倉 慎治, 松井 桂子, 川井 忠, 松井 有恒, 佐々木 信也, 越後 成志, 鈴木 治

    日本口腔外科学会雑誌　54　(Suppl.)　124-124　2008/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  247. リン酸オクタカルシウム・コラーゲン複合体(OCP/Collagen)のイヌ人工的顎裂部への応用

    松井 有恒, 松井 桂子, 鎌倉 慎治, 川井 忠, 佐々木 信也, 鈴木 治, 越後 成志

    日本口腔外科学会雑誌　54　(Suppl.)　124-124　2008/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  248. リン酸オクタカルシウム(OCP)含有量に依存したOCPコラーゲン複合体の骨再生能

    川井 忠, 穴田 貴久, 鎌倉 慎治, 松井 桂子, 松井 有恒, 越後 成志, 鈴木 治

    日本口腔外科学会雑誌　54　(Suppl.)　184-184　2008/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  249. リン酸オクタカルシウムは骨芽細胞分化を用量依存的に促進する

    穴田 貴久, 熊谷 崇, 本田 義知, 鎌倉 慎治, 鈴木 治

    Clinical Calcium　18　(7)　1030-1030　2008/06

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  250. Dose-dependent osteogenic effect of octacalcium phosphate on mouse bone marrow stromal cells Peer-reviewed

    Takahisa Anada, Takashi Kumagai, Yoshitomo Honda, Taisuke Masuda, Ryutaro Kamijo, Shinji Kamakura, Nobuhito Yoshihara, Tsunemoto Kuriyagawa, Hidetoshi Shimauchi, Osamu Suzuki

    TISSUE ENGINEERING PART A　14　(6)　965-978　2008/06

    DOI： 10.1089/ten.tea.2007.0339 　

    ISSN： 1937-3341

    eISSN： 1937-335X

  251. Analysis of appositional bone formation using a novel rat experimental model Peer-reviewed

    D. Nakajima, S. Kamakura, M. Nakamura, O. Suzuki, S. Echigo, Y. Sasano

    ORAL DISEASES　14　(4)　308-313　2008/05

    DOI： 10.1111/j.1601-0825.2007.01377.x 　

    ISSN： 1354-523X

  252. Induction of octacalcium phosphate by surface modification of TiO2 film prepared by electron cyclotron resonance plasma oxidation Peer-reviewed

    Yusuke Orii, Hiroshi Masumoto, Takashi Goto, Yoshitomo Honda, Takahisa Anada, Keiichi Sasaki, Osamu Suzuki

    Interface Oral Health Science 2007: Proceedings of the 2nd International Symposium for Interface Oral Health Science, Held in Sendai, Japan, Between 18 and 19 February, 2007　317-322　2008/04/14

    DOI： 10.1007/978-4-431-76690-2_69 　

    More details Close

    ISBN-10: 4431766898<br /> ISBN-13: 978-4431766896<br /> Springer<br />

  253. リン酸オクタカルシウム・コラーゲン複合体(OCP/Col)の人工的顎裂部への応用

    松井 桂子, 川井 忠, 鎌倉 慎治, 鈴木 治, 松井 有恒, 越後 成志, 半田 拓人

    日本口蓋裂学会雑誌　33　(2)　210-210　2008/04

    Publisher: (一社)日本口蓋裂学会

    ISSN： 0386-5185

    eISSN： 2186-5701

  254. Long-term results of bone regeneration with OCP-collagen composites

    Shinji Kamakura, Taisuke Masuda, Kazuo Sasaki, Seishi Echigo, Osamu Suzuki

    8th World Biomaterials Congress 2008　4　2048　2008

  255. Intramembranous bone reaction to synthetic ocp during hydroxyapatite deposition onto the OCP template

    Osamu Suzuki, Tazunu Kikawa, Takahisa Anada, Shinji Kamakura, Hideki Imaizumi

    8th World Biomaterials Congress 2008　1　209　2008

  256. Effect of octacalcium phosphate/collagenon composite on subperiosteal bone augmentation.

    MATSUI Aritsune, ANADA Takahisa, MASUDA Taisuke, KAMAKURA Shinji, ECHIGO Seishi, SUZUKI Osamu

    Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices　2008　(0)　45-45　2008

    Publisher: The Japanese Society for Dental Materials and Devices

    DOI： 10.14832/gsjsdmd.2008f.0.45.0 　

  257. Loading of mechanical stress to osteoblasts may induce cytokine cross talks between osteoblasts and monocytes/macrophages in a JNK/p38-dependent manner Peer-reviewed

    Hiroyuki Matsui, Naoto Fukuno, Keiichi Sasaki, Osamu Suzuki, Takayasu Kobayashi, Shinri Tamura

    2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008　379-380　2008

    DOI： 10.1109/MHS.2008.4752481 　

  258. Effects of mechanical strain on differentiation of osteoblastic cells Peer-reviewed

    Matsui A, Masuda T.L, Anada T, Takahashi I, Arai F, Takami M, Kamijo R, Fukuda T, Echigo S, Suzuki O

    2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008　475-480　2008

    DOI： 10.1109/MHS.2008.4752499 　

  259. Effect of octacalcium phosphate ionic dissolution products on osteoblastic cell differentiation Peer-reviewed

    Takahisa Anada, Akihiro Araseki, Shou Matsukawa, Tomokazu Yamasaki, Shinji Kamakura, Osamu Suzuki

    BIOCERAMICS, VOL 20, PTS 1 AND 2　361-363　31-+　2008

    DOI： 10.4028/www.scientific.net/KEM.361-363.31 　

    ISSN： 1013-9826

  260. Effect of oxidation pressure on controlling octacalcium phosphate nucleation on implant titanium by ECR plasma oxidation Peer-reviewed

    Yusuke Orii, Hiroshi Masumoto, Takashi Goto, Yoshitomo Honda, Takahisa Anada, Keiichi Sasaki, Osamu Suzuki

    BIOCERAMICS, VOL 20, PTS 1 AND 2　361-363　725-+　2008

    ISSN： 1013-9826

  261. Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite. Peer-reviewed

    Takashi Kumagai, Takahisa Anada, Yoshitomo Honda, Masamichi Takami, Ryutaro Kamijo, Hidetoshi Shimauchi, Osamu Suzuki

    BIOCERAMICS, VOL 20, PTS 1 AND 2　361-363　1025-+　2008

    ISSN： 1013-9826

  262. Bone regenerative property of octacalcium phosphate in mouse critical sized calvarial defects Peer-reviewed

    Yoshitomo Honda, Shinji Kamakura, Takashi Kumagai, Osamu Suzuki

    BIOCERAMICS, VOL 20, PTS 1 AND 2　361-363　1253-+　2008

    DOI： 10.4028/www.scientific.net/KEM.361-363.1253 　

    ISSN： 1013-9826

  263. Osteoconductivity of titania films prepared by electron-cyclotron-resonance plasma oxidation of implant titanium Peer-reviewed

    Hiroshi Masumoto, Takashi Goto, Yusuke Orii, Yoshitomo Honda, Osamu Suzuki, Keiichi Sasaki

    BIOCERAMICS, VOL 20, PTS 1 AND 2　361-363　717-+　2008

    ISSN： 1013-9826

  264. Differences of bone regeneration by various calcium phosphate/collagen composites Peer-reviewed

    Shinji Kamakura, Kazuo Sasaki, Yoshitomo Honda, Taisuke Masuda, Takahisa Anada, Tadashi Kawai, Aritsune Matsui, Keiko Matsui, Seishi Echigo, Osamu Suzuki

    BIOCERAMICS, VOL 20, PTS 1 AND 2　361-363　1229-+　2008

    DOI： 10.4028/www.scientific.net/KEM.361-363.1229 　

    ISSN： 1013-9826

  265. Development of a cell culture system loading cyclic mechanical strain to chondrogenic cells Peer-reviewed

    Taisuke Masuda, Ichiro Takahashi, Takahisa Anada, Fumihito Arai, Toshio Fukuda, Teruko Takano-Yamamoto, Osamu Suzuki

    JOURNAL OF BIOTECHNOLOGY　133　(2)　231-238　2008/01

    DOI： 10.1016/j.jbiotec.2007.08.007 　

    ISSN： 0168-1656

    eISSN： 1873-4863

  266. The primacy of octacalcium phosphate collagen composites in bone regeneration Peer-reviewed

    Shinji Kamakura, Kazuo Sasaki, Takahiro Homma, Yoshitomo Honda, Takahisa Anada, Seishi Echigo, Osamu Suzuki

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A　83A　(3)　725-733　2007/12

    DOI： 10.1002/jbm.a.31332 　

    ISSN： 1549-3296

  267. OCP/Collagen複合体の骨再生能における優位性

    鎌倉 慎治, 佐々木 和夫, 本間 崇裕, 本田 義知, 穴田 貴久, 越後 成志, 鈴木 治

    日本バイオマテリアル学会大会予稿集　29回　211-211　2007/11

    Publisher: 日本バイオマテリアル学会

  268. リン酸オクタカルシウムの結晶性は骨髄間質細胞の接着と増殖を制御する

    本田 義知, 穴田 貴久, 鎌倉 慎治, 鈴木 治

    日本バイオマテリアル学会大会予稿集　29回　376-376　2007/11

    Publisher: 日本バイオマテリアル学会

  269. リン酸オクタカルシウム(OCP)は、コラーゲン担体中で用量依存的にラット頭蓋冠骨欠損修復を促進する

    川井 忠, 穴田 貴久, 鎌倉 慎治, 本田 義知, 松井 有恒, 佐々木 和夫, 越後 成志, 鈴木 治

    日本バイオマテリアル学会大会予稿集　29回　210-210　2007/11

    Publisher: 日本バイオマテリアル学会

  270. Dehydrothermal treatment of collagen influences on bone regeneration by octacalcium phosphate (OCP) collagen composites Peer-reviewed

    Shinji Kamakura, Kazuo Sasaki, Yoshitomo Honda, Takahisa Anada, Keiko Matsui, Seishi Echigo, Osamu Suzuki

    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE　1　(6)　450-456　2007/11

    DOI： 10.1002/term.58 　

    ISSN： 1932-6254

  271. Conversion of Octacalcium Phosphate into Hydroxyapatite and Bone Regeneration Peer-reviewed

    Suzuki O Kamakura, S Anada T

    Key Engineering Materials　in press　2007/10

  272. Proliferation and adhesion of periodontal ligament cells on synthetic biominerals Peer-reviewed

    J. Hatakeyama, Y. Hatakeyama, I. Takahashi, O. Suzuki, Y. Sasano

    ORAL DISEASES　13　(5)　500-506　2007/09

    DOI： 10.1111/j.1601-0825.2006.01333.x 　

    ISSN： 1354-523X

  273. Creating a Hydroxyapatite Film on Human Enamel by Powder Jet Deposition Technique Peer-reviewed

    Miyoko Noji, Mohammad Saeed, Sepasy Zahmaty, Toshihiko Shibuya, Nobuhito Yoshihara, Tsunemoto Kuriyagawa, Keiichi Sasaki, Osamu Suzuki

    Proceedings of the 10th International Symposium on Advances in Abrasive Technology　663-670　2007/09

  274. 骨再生担体材料OCPの物理化学的因子は骨形成能を左右する

    本田 義知, 穴田 貴久, 鎌倉 慎治, 鈴木 治

    Journal of Oral Biosciences　49　(Suppl.)　203-203　2007/08

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  275. イヌ頭蓋骨欠損部へ埋入したOCP/Collagen複合体の定量的骨再生能評価

    川井 忠, 松井 桂子, 佐々木 信也, 鎌倉 慎治, 鈴木 治, 越後 成志

    日本口腔外科学会雑誌　53　(Suppl.)　52-52　2007/08

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  276. リン酸カルシウム・コラーゲン複合体間での骨再生能の比較検討

    鎌倉 慎治, 本間 崇裕, 松井 桂子, 川井 忠, 佐々木 信也, 松井 有恒, 越後 成志, 鈴木 治

    日本口腔外科学会雑誌　53　(Suppl.)　53-53　2007/08

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  277. リン酸オクタカルシウムによる象牙芽細胞分化誘導と象牙質形成

    王 シアオグ, 須澤 徹夫, 舘 慶太, 中村 雅典, 本田 義知, 穴田 貴久, 鈴木 治, 上條 竜太郎

    Journal of Oral Biosciences　49　(Suppl.)　102-102　2007/08

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  278. 合成リン酸オクタカルシウム(OCP)の骨再生能について

    鈴木 治, 今泉 秀樹, 鎌倉 慎治, 穴田 貴久, 益田 泰輔

    日本歯科産業学会誌　21　(1)　58-58　2007/06

    Publisher: 日本歯科産業学会

    ISSN： 1342-3606

  279. イヌ頭蓋骨欠損部へ埋入したリン酸オクタカルシウム・コラーゲン複合体は骨再生を促進する

    川井 忠, 佐々木 信也, 松井 桂子, 佐々木 和夫, 鎌倉 慎治, 鈴木 治, 越後 成志

    Clinical Calcium　17　(5)　796-796　2007/04

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  280. Differentiation and monitoring of cells using a biochip for regenerative medicine. (Differentiation of bone marrow stromal cells and myoblasts) Invited Peer-reviewed

    Tomoyuki Uchida, Fumihito Arai, Osamu Suzuki, Akihiko Ichikawa, Toshio Fukuda, Takenobu Katagiri, Ryutaro Kamijo, Masanori Nakamura, Mamoru Numata, Naruaki Watanabe

    JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing　49　(3)　852-858　2007/03/15

    DOI： 10.1299/jsmec.49.852 　

    ISSN： 1344-7653 1347-538X

  281. リン酸オクタカルシウム(OCP)による硬組織再生誘導メカニズムの検討

    高見 正道, 須澤 徹夫, 上條 竜太郎, 望月 文子, 川和 忠治, 王 こう谷, 松本 光吉, 中村 雅典, 鎌倉 慎治, 鈴木 治

    昭和歯学会雑誌　27　(1)　53-54　2007/03

    Publisher: 昭和大学・昭和歯学会

    ISSN： 0285-922X

  282. Formation of bone-like apatite enhanced by hydrolysis of octacalcium phosphate crystals deposited in collagen matrix Peer-reviewed

    Y. Honda, S. Kamakura, K. Sasaki, O. Suzuki

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS　80B　(2)　281-289　2007/02

    DOI： 10.1002/jbm.b.30595 　

    ISSN： 1552-4973

  283. Biological response of Octacalcium Phosphate Combined with collagen implanted in subperiosteal region

    Suzuki Yuko, Kamakura Shinji, Hatori Kouki, Sasaki Kazuo, Honda Yoshitomo, Anada Takahisa, Sasaki Keiichi, Suzuki Osamu

    Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices　2007　(0)　39-39　2007

    Publisher: The Japanese Society for Dental Materials and Devices

    DOI： 10.14832/gsjsdmd.2007f.0.39.0 　

    More details Close

    Previous studies showed that octacalcium phosphate (OCP) combined with collagen (OCP/Col) enhances bone regeneration in bone defect. In the present study, OCP/Col disc (1 mm or 3 mm in thickness) were implanted into the periosteal region in rat crania to investigate the bone regenerative properties of OCP/Col. The maximum thickness of the bone with implant was measured parallel to the calvaria. The histological sections were cut and activities of tartrate resistant acid phosphatase (TRAP) were stained to examine phagocytotic multinucleated giant cells. The effect of implantation on crystalline phase of OCP in the implant was examined by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The present study suggests that new bone formation on OCP/Col and biodegradation of OCP/Col are depending on the thickness of OCP/Col.

  284. Fabrication of PDMS scaffold with controlled configurations Peer-reviewed

    Taisuke Masuda, Takahisa Anada, Ichiro Takahashi, Fumihito Arai, Toshio Fukuda, Osamu Suzuki

    2007 INTERNATIONAL SYMPOSIUM ON MICRO-NANO MECHATRONICS AND HUMAN SCIENCE, VOLS 1 AND 2　362-+　2007

    ISSN： 2474-378X

    eISSN： 2474-3798

  285. Mechanobiological signal transduction in differentiating chondrocyte and new configuration for mechanical stress culture Peer-reviewed

    Ichiro Takahashi, Taisuke Masuda, Fumihito Arai, Takahisa Anada, Toshio Fukuda, Osamu Suzuki, Teruko Takano-Yamamoto

    2007 2ND IEEE INTERNATIONAL CONFERENCE ON NANO/MICRO ENGINEERED AND MOLECULAR SYSTEMS, VOLS 1-3　827-+　2007

  286. The magnitude and duration of cyclic stretch affect JNK and p38 activation in MC3T3-E1 pre-osteoblastic cells Peer-reviewed

    Hiroyuki Matsui, Naoto Fukuno, Keiichi Sasaki, Osamu Suzuki, Takayasu Kobayashi, Shinri Tamura

    2007 INTERNATIONAL SYMPOSIUM ON MICRO-NANO MECHATRONICS AND HUMAN SCIENCE, VOLS 1 AND 2　183-+　2007

    ISSN： 2474-378X

    eISSN： 2474-3798

  287. Bone regenerative property of synthetic octacalcium phosphate in collagen matrix. Peer-reviewed

    Tadashi Kawai, Takahisa Anada, Shinji Kamakura, Yoshitomo Honda, Aritsune Matsui, Kazuo Sasaki, Seishi Echigo, Osamu Suzuki

    Interface Oral Health Science　2007

  288. OCP/Collagen複合体はイヌ頭蓋の臨界骨欠損における骨再生を効果的に促進する Peer-reviewed

    川井 忠, 松井 桂子, 飯淵 信也, 佐々木 和夫, 鎌倉 慎治, 鈴木 治, 越後 成志

    Orthopaedic Ceramic Implants　27　2007

  289. Preparation of titania films on implant titanium by electron cyclotron resonance plasma oxidation Peer-reviewed

    Hiroshi Masumoto, Takashi Goto, Yoshitomo Honda, Osamu Suzuki, Keiichi Sasaki

    BIOCERAMICS, VOL 19, PTS 1 AND 2　330-332　565-+　2007

    ISSN： 1013-9826

  290. DCPD/collagen scaffold and apatite formation in vitro Peer-reviewed

    Y. Honda, S. Kamakura, K. Sasaki, T. Anada, T. Masuda, O. Suzuki

    BIOCERAMICS, VOL 19, PTS 1 AND 2　330-332　479-+　2007

    ISSN： 1013-9826

  291. Osteoconductivity of porous titanium having Young's modulus similar to bone and surface modification by OCP Peer-reviewed

    Yuko Suzuki, Naoyuki Nomura, Shuji Hanada, Shinji Kamakura, Takahisa Anada, Takeshi Fuji, Yoshitomo Honda, Taisuke Masuda, Keiichi Sasaki, Shoichi Kokubun, Osamu Suzuki

    BIOCERAMICS, VOL 19, PTS 1 AND 2　330-332　951-+　2007

    DOI： 10.4028/www.scientific.net/KEM.330-332.951 　

    ISSN： 1013-9826

  292. Stability of regenerated bone by octacalcium phosphate (OCP) combined with collagen Peer-reviewed

    Shinji Kamakura, Kazuo Sasaki, Yoshitomo Honda, Takahisa Anada, Tadashi Kawai, Keiko Matsui, Seishi Echigo, Osamu Suzuki

    BIOCERAMICS, VOL 19, PTS 1 AND 2　330-332　(332)　1315-+　2007

    DOI： 10.4028/www.scientific.net/KEM.330-332.1315 　

    ISSN： 1013-9826

  293. Bone regeneration with a transitory scaffold modifying local environment

    S. Kamakura, O. Suzuki, Y. Honda, R. Kamijo, M. Nakamura, I. Takahashi, K. Sasaki, M. Oda, F. Arai, T. Fukuda

    2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS　2006/12/01

    DOI： 10.1109/MHS.2006.320329 　

  294. OCP-コラーゲン複合材料による骨再生能とコラーゲン架橋処理

    鎌倉 慎治, 佐々木 和夫, 本田 義知, 穴田 貴久, 松井 桂子, 越後 成志, 鈴木 治

    日本バイオマテリアル学会大会予稿集　28回　129-129　2006/11

    Publisher: 日本バイオマテリアル学会

  295. 骨芽細胞様細胞の分化に及ぼすリン酸オクタカルシウムの影響

    穴田 貴久, 熊谷 崇, 本田 義知, 鎌倉 慎治, 鈴木 治

    日本バイオマテリアル学会大会予稿集　28回　348-348　2006/11

    Publisher: 日本バイオマテリアル学会

  296. Octacalcium phosphate combined with collagen orthotopically enhances bone regeneration Peer-reviewed

    Shinji Kamakura, Kazuo Sasaki, Yoshitomo Honda, Takahisa Anada, Osamu Suzuki

    Journal of Biomedical Materials Research - Part B Applied Biomaterials　79　(2)　210-217　2006/11

    DOI： 10.1002/jbm.b.30531 　

    ISSN： 0021-9304 1552-4981

    eISSN： 1552-4981

  297. Octacalcium phosphate combined with collagen orthotopically enhances bone regeneration Peer-reviewed

    Shinji Kamakura, Kazuo Sasaki, Yoshitomo Honda, Takahisa Anada, Osamu Suzuki

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS　79B　(2)　210-217　2006/11

    DOI： 10.1002/jbm.b.30531 　

    ISSN： 1552-4973

    eISSN： 1552-4981

  298. 細胞外カルシウムのマクロファージ様細胞が持つ潜在的骨形成能促進効果

    本田 義知, 穴田 貴久, 鎌倉 慎治, 中村 雅典, 菅原 俊二, 鈴木 治

    Journal of Oral Biosciences　48　(Suppl.)　160-160　2006/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  299. リン酸オクタカルシウムは量依存的に培養骨芽細胞様細胞の増殖・分化に影響を与える

    熊谷 崇, 穴田 貴久, 本田 義知, 鎌倉 慎治, 島内 英俊, 鈴木 治

    Journal of Oral Biosciences　48　(Suppl.)　160-160　2006/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  300. OCP-Collagen複合体の人工的骨欠損部への応用

    松井 桂子, 鎌倉 慎治, 川井 忠, 佐々木 信也, 鈴木 治, 越後 成志

    日本口腔外科学会雑誌　52　(Suppl.)　163-163　2006/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  301. Erratum to "Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line" [Biochem. Biophys. Res. Commun. 345 (2006) 1155-1160] (DOI:10.1016/j.bbrc.2006.05.013) Peer-reviewed

    Yoshitomo Honda, Takahisa Anada, Shinji Kamakura, Masanori Nakamura, Shunji Sugawara, Osamu Suzuki

    Biochemical and Biophysical Research Communications　347　(3)　843　2006/09/01

    DOI： 10.1016/j.bbrc.2006.06.124 　

    ISSN： 0006-291X 1090-2104

    eISSN： 1090-2104

  302. 骨再生におけるOctacalcium phosphate(OCP)とCollagenの複合効果

    鎌倉 慎治, 佐々木 和夫, 本田 義知, 穴田 貴久, 鈴木 治

    Clinical Calcium　16　(9)　1560-1561　2006/08

    Publisher: (株)医薬ジャーナル社

    ISSN： 0917-5857

  303. Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line Invited Peer-reviewed

    Y Honda, T Anada, S Kamakura, M Nakamura, S Sugawara, O Suzuki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　345　(3)　1155-1160　2006/07

    DOI： 10.1016/j.bbrc.2006.05.013 　

    ISSN： 0006-291X

    eISSN： 1090-2104

  304. Bone formation enhanced by implanted octacalcium phosphate involving conversion into Ca-deficient hydroxyapatite Peer-reviewed

    O Suzuki, S Kamakura, T Katagiri, M Nakamura, BH Zhao, Y Honda, R Kamijo

    BIOMATERIALS　27　(13)　2671-2681　2006/05

    DOI： 10.1016/j.boimaterials.2005.12.004 　

    ISSN： 0142-9612

  305. Octacalcium phosphate(OCP)/Collagen複合体による骨再生促進

    鎌倉 慎治, 本田 義知, 穴田 貴久, 鈴木 治

    歯科材料・器械　25　(2)　139-139　2006/04

    Publisher: (一社)日本歯科理工学会

    ISSN： 0286-5858

  306. Surface chemistry and biological responses to synthetic octacalcium phosphate Peer-reviewed

    Osamu Suzuki, Shinji Kamakura, Takenobu Katagiri

    Journal of Biomedical Materials Research - Part B Applied Biomaterials　77　(1)　201-212　2006/04

    DOI： 10.1002/jbm.b.30407 　

    ISSN： 0021-9304 1552-4981

    eISSN： 1552-4981

  307. Characterization of dentin formed in transplanted rat molars by electron probe microanalysis Peer-reviewed

    N Akiba, Y Sasano, O Suzuki, K Sasaki

    CALCIFIED TISSUE INTERNATIONAL　78　(3)　143-151　2006/03

    DOI： 10.1007/s00223-005-0134-4 　

    ISSN： 0171-967X

  308. Fabrication and estimation of biomimetic scaffold combining octacalcium phosphate with collagen

    Honda Yoshitomo, Kamakura Sinji, Suzuki Osamu

    Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices　2006　(0)　41-41　2006

    Publisher: The Japanese Society for Dental Materials and Devices

    DOI： 10.14832/gsjsdmd.2006s.0.41.0 　

    More details Close

    This study was designed to investigate whether formation of bone like apatite is accelerated by calcium phosphate deposited with collagen when immersed in simulated body fluid. The apatite formation was proved within one day.

  309. Enhancement of bone regeneration by the implantation of OCP-Collagen composite

    KAMAKURA SHINJI, HONDA YOSHITOMO, ANADA TAKAHISA, SUZUKI OSAMU

    Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices　2006　(0)　19-19　2006

    Publisher: The Japanese Society for Dental Materials and Devices

    DOI： 10.14832/gsjsdmd.2006s.0.19.0 　

  310. Proliferation and differentiation of osteoblastic cells on octacalcium phosphate

    Anada Takahisa, Honda Yoshitomo, Kamakura Shinji, Suzuki Osamu

    Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices　2006　(0)　62-62　2006

    Publisher: The Japanese Society for Dental Materials and Devices

    DOI： 10.14832/gsjsdmd.2006f.0.62.0 　

    More details Close

    The present study was designed to investigate whether octacalcium phosphate (OCP) affects proliferation and differentiation of osteoblastic cells. Mouse bone marrow stromal ST-2 cells were cultured on the dishes pre-coated with OCP or hydroxyapatite (HA). The proliferation of ST-2 cultured on OCP was initially inhibited, whereas their differentiation to osteoblasts was promoted at last.

  311. A new configuration device for mechanical stress culture Peer-reviewed

    Taisuke Masuda, Ichiro Takahashi, Fumihito Arai, Takahisa Anada, Yoichi Miyamoto, Toshio Fukuda, Osamu Suzuki

    2006 IEEE INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE　214-+　2006

    ISSN： 2474-378X

    eISSN： 2474-3798

  312. Comparative study on osteoconductivity by synthetic octacalcium phosphate and sintered hydroxyapatite in rabbit bone marrow Peer-reviewed

    H Imaizumi, M Sakurai, O Kashimoto, T Kikawa, O Suzuki

    CALCIFIED TISSUE INTERNATIONAL　78　(1)　45-54　2006/01

    DOI： 10.1007/s00223-005-0170-0 　

    ISSN： 0171-967X

  313. Fluoridated Ca-deficient apatite formed via octacalcium phosphate enhances bone regeneration Peer-reviewed

    S Kamakura, T Kumagai, Y Honda, T Anada, K Sasaki, H Shimauchi, O Suzuki

    BIOCERAMICS 18, PTS 1 AND 2　309-311　137-140　2006

    ISSN： 1013-9826

  314. Effects of mechanical stress and scaffold material on osteogenesis and chondrogenesis

    O. Suzuki, I. Takahashi, S. Kamakura, K. Sasaki, R. Kamijo, M. Nakamura, M. Oda, T. Uchida, F. Arai, T. Fukuda

    Proceedings of the 2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Progr　2005　235-240　2005/12/01

    DOI： 10.1109/MHS.2005.1589996 　

  315. Octacalcium phosphate(OCP)/Collagen複合体による骨再生

    鎌倉 慎治, 本田 義知, 穴田 貴久, 鈴木 治

    Journal of Oral Biosciences　47　(Suppl.)　82-82　2005/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  316. 新規生体骨組織模倣材料による骨再生

    鎌倉 慎治, 鈴木 治

    日本口腔外科学会雑誌　51　(Suppl.)　135-135　2005/09

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  317. Creating new interface between tooth and biomaterials using powder-jet technology Peer-reviewed

    M.Noji, K.Sasaki, T.Kuriyagawa, O.Suzuki, K.Ogawa, M.Kanehira

    International Congress Series　1284　302-304　2005/09

    DOI： 10.1016/j.ics.2005.07.009 　

  318. Synthetic octacalcium phosphate (OCP) is an effective scaffold to regenerate bone Peer-reviewed

    Kamakura, S, Sasano, Y, Suzuki, O

    Int. Congr. Ser.　1284　290-295　2005

    DOI： 10.1016/j.ics.2005.06.035 　

    ISSN： 0531-5131

  319. Differentiation and monitoring of cells using a biochip for regenerative medicine. Peer-reviewed

    Arai, F, Suzuki, O, Uchida, T, Ichikawa, A, Fukuda, T, Katagiri, T, Kamijo, R, Nakamura, M, Numata, M, Watanabe, N

    Transaction of the Japanese Society of Mechanical Engineers　71　(C)　3239-3245　2005

    DOI： 10.1299/kikaic.71.3239 　

  320. In vitro mechanical cell loading system for regenerative medicine Peer-reviewed

    F Arai, O Suzuki, T Uchida, A Ichikawa, T Fukuda, R Kamijor, T Katagiri, M Nakamura, M Numata, N Watanabe

    Micro Total Analysis Systems 2004, Vol 1　1　(296)　360-362　2005

    ISSN： 0260-6291

  321. 新規生体置換型材料を用いた骨再生

    鎌倉 慎治, 鈴木 治, 笹野 泰之

    日本口腔外科学会雑誌　50　(13)　851-852　2004/12

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

  322. Multiple imaging techniques demonstrate the manipulation of surfaces to reduce bacterial contamination and corrosion Peer-reviewed

    JW Arnold, DH Boothe, O Suzuki, GW Bailey

    JOURNAL OF MICROSCOPY-OXFORD　216　(Pt 3)　215-221　2004/12

    DOI： 10.1111/j.0022-2720.2004.01415.x 　

    ISSN： 0022-2720

  323. New scaffold for recombinant human bone morphogenetic protein-2 Peer-reviewed

    S Kamakura, S Nakajo, O Suzuki, Y Sasano

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A　71A　(2)　299-307　2004/11

    DOI： 10.1002/jbm.a.30157 　

    ISSN： 0021-9304

  324. Design criteria and evaluation for pharmaceutical containment systems. Evaluation for closed isolation systems Peer-reviewed

    Suzuki O, Takeda M, Tanaka K, Numata M

    Pharmaceutical Engineering　24　44-58　2004/04

  325. ケミカルハザード対応医薬品製造施設・設備における封じ込め性能の定量的評価

    渡辺成章, 鈴木治, 竹田守彦, 沼田守

    クリーンテクノロジー　2　10-15　2004/04

  326. Design criteria and evaluation of pharmaceutical containment systems. Evaluation for open isolation systems Peer-reviewed

    Suzuki O, Takeda M, Tanaka K, Inoue M

    Pharmaceutical Engineering　23　66-78　2003/04

  327. Design criteria and evaluation for pharmaceutical containment systems in aseptic dosage form manufacturing facilities Peer-reviewed

    Suzuki O, Tanaka K, Watanabe N, Takeda M

    Pharmaceutical Technology, Aseptic Processing (Supplement)　suppl　24-31　2003/04

  328. Effects of corrosive treatment on stainless steel surface finishes and bacterial attachment Peer-reviewed

    Arnold JW, Suzuki O

    Transaction of the American Society of Agricultural Engineers (ASAE)　46　1595-1602　2003/04

  329. ケミカルハザード対応医薬品製造施設・設備の封じ込めレベル評価 (2)., 無菌製剤製造工場におけるオープン系アイソレーションシステムの例

    鈴木治, 田中浩二, 渡辺成章, 竹田守彦

    PHARM TECH JAPAN　19　59-68　2003/04

  330. Coke deposition in furnace tubes of the vacuum distillation unit estimated by stability of atmospheric residue oil Peer-reviewed

    Suzuki O, Yamano S, Konishi H, Umino H, Inomata M

    J Jpn Petrol Inst　45　207-213　2002/04

    DOI： 10.1627/jpi.45.207 　

  331. ケミカルハザード対応医薬品製造施設・設備の封じ込めレベル評価(1). 非無菌原薬製造工場におけるオープン系アイソレーションシステムの例

    鈴木治, 竹田守彦

    PHARM TECH JAPAN　18　31-39　2002/04

  332. Implanted octacalcium phosphate is more resorbable than beta-tricalcium phosphate and hydroxyapatite Peer-reviewed

    S Kamakura, Y Sasano, T Shimizu, K Hatori, O Suzuki, M Kagayama, K Motegi

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH　59　(1)　29-34　2002/01

    ISSN： 0021-9304

  333. Implanted octacalcium phosphate is more resorbable than β-tricalcium phosphate and hydroxyapatite Peer-reviewed

    S. Kamakura, Y. Sasano, T. Shimizu, K. Hatori, O. Suzuki, M. Kagayama, K. Motegi

    Journal of Biomedical Materials Research　59　(1)　29-34　2002

    DOI： 10.1002/jbm.1213 　

    ISSN： 0021-9304

  334. Implantation of octacalcium phosphate combined with transforming growth factor-beta(1) enhances bone repair as well as resorption of the implant in rat skull defects Peer-reviewed

    S Kamakura, Y Sasano, S Nakajo, T Shimizu, O Suzuki, F Katou, M Kagayama, K Motegi

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH　57　(2)　175-182　2001/11

    DOI： 10.1002/1097-4636(200111)57:2<175::AID-JBM1156>3.0.CO;2-Q 　

    ISSN： 0021-9304

  335. Implantation of octacalcium phosphate nucleates isolated bone formation in rat skull defects Peer-reviewed

    S Kamakura, Y Sasano, H Homma, O Suzuki, M Kagayama, K Motegi

    ORAL DISEASES　7　(4)　259-265　2001/07

    DOI： 10.1034/j.1601-0825.2001.0070410.x 　

    ISSN： 1354-523X

  336. Implantation of octacalcium phosphate (OCP) in rat skull defects enhances bone repair Peer-reviewed

    S Kamakura, Y Sasano, H Homma, O Suzuki, M Kagayama, K Motegi

    JOURNAL OF DENTAL RESEARCH　78　(11)　1682-1687　1999/11

    ISSN： 0022-0345

  337. Implanted octacalcium phosphate (OCP) stimulates osteogenesis by osteoblastic cells and/or committed osteoprogenitors in rat calvarial periosteum Peer-reviewed

    Y Sasano, S Kamakura, H Homma, O Suzuki, Mizoguchi, I, M Kagayama

    ANATOMICAL RECORD　256　(1)　1-6　1999/09

    DOI： 10.1002/(SICI)1097-0185(19990901)256:1<1::AID-AR1>3.0.CO;2-X 　

    ISSN： 0003-276X

  338. Adsorption of benzoic compounds onto stainless steel particles Peer-reviewed

    Osamu Suzuki, Yoshinaru Shibata, Mikio Inoue

    Journal of Colloid and Interface Science　193　(2)　234-241　1997/09/15

    Publisher: Academic Press Inc.

    DOI： 10.1006/jcis.1997.5038 　

    ISSN： 0021-9797

  339. Multinucleated giant cells recruited by implantation of octacalcium phosphate (OCP) in rat bone marrow share ultrastructural characteristics with osteoclasts Peer-reviewed

    S Kamakura, Y Sasano, H HommaOhki, M Nakamura, O Suzuki, M Kagayama, K Motegi

    JOURNAL OF ELECTRON MICROSCOPY　46　(5)　397-403　1997

    ISSN： 0022-0744

  340. Initiation of alveolar ridge augmentation in the rat mandible by subperiosteal implantation of octacalcium phosphate Peer-reviewed

    S Kamakura, Y Sasano, M Nakamura, O Suzuki, H Ohki, M Kagayama, K Motegi

    ARCHIVES OF ORAL BIOLOGY　41　(11)　1029-1038　1996/11

    DOI： 10.1016/S0003-9969(96)00080-5 　

    ISSN： 0003-9969

  341. Experimental study of alveolar augmentation with a mixture of octacalcium phosphate and hydroxyapatite

    KAMAKURA Shinji, SASANO Yasuyuki, OHKI Hidetaka, SUZUKI Osamu, KAGAYAMA Manabu, MOTEGI Katsutoshi

    Journal of Oral Surgery Society of Japan　42　(10)　1043-1047　1996/10/20

    Publisher: Japanese Society of Oral and Maxillofacial Surgeons

    DOI： 10.5794/jjoms.42.1043 　

    ISSN： 0021-5163

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    The present study was designed to investigate the process of bone formation induced by implantation of a mixture of octacalcium phosphate (OCP) and hydroxyapatite (HA)(OCP/HA) in the alveolar ridge of the rat mandible. Synthetic OCP and HA (weight ratio 1: 1) were mixed and implanted into the subperiosteal pocket of the rat mandible. Bone formation at the alveolar ridge was examined radiographically and histologically between 1 and 8 weeks after implantation. Granular radiopacity of the OCP/HA implant was obvious at week 1. Osteogenesis was initiated from the bone surface near the implantation site of the mandible at week 1 and some implanted OCP and HA were directly enclosed by newly formed bone at week 4. The present study suggests that the OCP/HA mixture could be used to augment the atrophic alveolar ridge.

  342. Reversible structural changes of octacalcium phosphate and labile acid phosphate Peer-reviewed

    O Suzuki, H Yagishita, T Amano, T Aoba

    JOURNAL OF DENTAL RESEARCH　74　(11)　1764-1769　1995/11

    ISSN： 0022-0345

  343. SUBPERIOSTEAL IMPLANTATION OF OCTACALCIUM PHOSPHATE (OCP) STIMULATES BATH CHONDROGENESIS AND OSTEOGENESIS IN THE TIBIA, BUT ONLY OSTEOGENESIS IN THE PARIETAL BONE OF A RAT Peer-reviewed

    Y SASANO, S KAMAKURA, M NAKAMURA, O SUZUKI, MIZOGUCHI, I, H AKITA, M KAGAYAMA

    ANATOMICAL RECORD　242　(1)　40-46　1995/05

    DOI： 10.1002/ar.1092420106 　

    ISSN： 0003-276X

  344. 燐酸オクタカルシウム(OCP)およびハイドロキシアパタイト(HA)を用いた歯槽堤増大に関する実験的研究 Peer-reviewed

    鎌倉 慎治, 大木英孝, 茂木克俊, 笹野泰之, 中村雅典, 田辺 元, 鈴木 治

    日本口腔科学会雑誌　42　(5)　1004-1004　1993/12

    Publisher: (NPO)日本口腔科学会

    ISSN： 0029-0297

    eISSN： 2185-0461

  345. MACLURA-POMIFERA AGGLUTININ-BINDING GLYCOCONJUGATES ON CONVERTED APATITE FROM SYNTHETIC OCTACALCIUM PHOSPHATE IMPLANTED INTO SUBPERIOSTEAL REGION OF MOUSE CALVARIA Peer-reviewed

    O SUZUKI, M NAKAMURA, Y MIYASAKA, M KAGAYAMA, M SAKURAI

    BONE AND MINERAL　20　(2)　151-166　1993/02

    ISSN： 0169-6009

  346. Experimental study on alveolar augumentation with octacalcium phosphate.

    KAMAKURA Shinji, SASANO Yasuyuki, NAKAMURA Masanori, TANABE Gen, SUZUKI Osamu, OOKI Hidetaka, MOTEGI Katsutoshi

    Journal of Oral Surgery Society of Japan　39　(12)　1288-1294　1993

    Publisher: Japanese Society of Oral and Maxillofacial Surgeons

    DOI： 10.5794/jjoms.39.1288 　

    ISSN： 0021-5163

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    Progressive resorption of the alveolar ridge after tooth extraction results in diminished retention and stability of a denture. Preprosthetic augumentation of the alveolar ridge is therefore required to provide a firm base for a denture and reconstruct the functional occlusion in the field of oral and maxillofacial surgery.<BR>Octacalcium phosphate (OCP), the putative precursor of biological hydroxyapatite, was implanted into the subperiosteal pocket adjacent to the mandibular alveolar ridge in 7-week-old male Wistar rats. Soft radiographic and the histological examinations were performed to investigate the biological response to the OCP implant and assess possibilities for the clinical application.<BR>OCP was prepared using the method of LeGeros; granules between 32 and 48 mesh were used for implantation. Four rats were used in each experimental group. The animals were perfused with 4% paraformaldehyde in 0.1 M phosphate buffer at pH 7.4. After soft radiographic examination, the specimens were decalcified in a mixed solution of sodium citrate and formic acid, embedded in paraffin, sliced into thin sections, and stained with hematoxylin and eosin.<BR>In the experimental group, signs of radiopacity were not obvious at the implantation site at one week. At two weeks, increased signs of radiopacity were noted at the implantation site. The radiopacity increased markedly by four weeks after implantation and seemed to be relatively homogeneous, well amalgamated to the contiguous bone. Bone resorption was not obvious at the implantation site after eight weeks.<BR>Histologically, the implanted-OCP (i-OCP) was stained by hematoxylin at one week. The ridge was augumented at the region corresponding to the implantation site. Numerous connective tissue cells and inflammatory cells were surrounding i-OCP. Additional bone formation increased with time. I-OCP tended to aggregate and be resorbed. Two weeks after implantation, inflammatory cells decreased. After four weeks, i-OCP was surrounded by new bone. The newly formed bone was observed to mature with time and was not resorbed after eight weeks. The present study suggested that OCP is a useful material to augument the atrophic alveolar ridge in preprosthetic surgery.

  347. BONE-FORMATION ON SYNTHETIC PRECURSORS OF HYDROXYAPATITE Peer-reviewed

    O SUZUKI, M NAKAMURA, Y MIYASAKA, M KAGAYAMA, M SAKURAI

    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE　164　(1)　37-50　1991/05

    DOI： 10.1620/tjem.164.37 　

    ISSN： 0040-8727

    eISSN： 1349-3329

Show all ︎Show first 5

Misc. 117

  1. 生体内リン酸八カルシウムによる骨分化誘導効果

    奥山喬介, 奥山喬介, 塩飽由香利, 塩飽由香利, 濱井瞭, 溝口利英, 土屋香織, 高橋哲, 鈴木治

    日本再生医療学会総会(Web)　21st　2022

  2. リン酸八カルシウム周囲の間葉系幹細胞の局在と分化

    奥山喬介, 奥山喬介, 塩飽由香利, 塩飽由香利, 濱井瞭, 溝口利英, 土屋香織, 高橋哲, 鈴木治

    日本口腔科学会学術集会プログラム・抄録集　76th　2022

  3. Effects of octacalcium phosphate on mouse bone marrow-derived mesenchymal stem cell differentiation and bone regeneration.

    奥山喬介, 奥山喬介, 奥山喬介, 塩飽由香利, 濱井瞭, 溝口利英, 土屋香織, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　44th　2022

  4. 新規骨補塡材料 リン酸八カルシウム・コラーゲン複合体「ボナーク」について

    川井 忠, 髙橋 哲, 松井 桂子, 鈴木 治, 鎌倉 慎治

    81　(5)　105-114　2021/05

    ISSN： 0289-0909

  5. Role of mesenchymal stem cells in bone regeneration by octacalcium phosphate

    奥山喬介, 塩飽由香利, 塩飽由香利, 濱井瞭, 溝口利英, 高橋哲, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　43rd　2021

  6. Bone regeneration and osteocyte differentiation induced by octacalcium phosphate (OCP)

    塩飽由香利, 塩飽由香利, 齊藤志都, 濱井瞭, 長谷川智香, 網塚憲生, 蔡優広, 高橋哲, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　43rd　2021

  7. リン酸八カルシウム(OCP)が新生骨基質の骨細胞分化に及ぼす影響の検討

    齊藤志都, 齊藤志都, 塩飽由香利, 濱井瞭, 長谷川智香, 網塚憲生, 高橋哲, 鈴木治

    日本再生医療学会総会(Web)　20th　2021

  8. Analysis of osteocyte differentiation during bone formation by OCP implantation

    齊藤志都, 塩飽由香利, 濱井瞭, 高橋哲, 鈴木治, 長谷川智香, 網塚憲生

    日本セラミックス協会年会講演予稿集(CD-ROM)　2020　2020

  9. Performance of an octacalcium phosphate biomaterial in a long bone defect in ovariectomized rat

    Kazuyoshi Baba, Yukari Shiwaku, Takahisa Anada, Yu Mori, Kaori Tsuchiya, Eiji Itoi, Osamu Suzuki

    Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium　40　932　2019/01/01

    ISSN： 1526-7547

  10. リン酸オクタカルシウムによって誘起される骨再生における早期組織応答

    黒羽根壮, 穴田貴久, 塩飽由香利, 濱井瞭, 飯久保正弘, 高橋哲, 鈴木治

    セラミックス基礎科学討論会講演要旨集　57th　206　2019/01

  11. 骨粗鬆症モデルラットによるリン酸オクタカルシウムの骨組織応答性に関する研究

    馬場一慈, 塩飽由香利, 穴田貴久, 濱井瞭, 森優, 井樋栄二, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　40th　218 (WEB ONLY)　2018

  12. 調製方法の異なるOCP/Gel複合体のラット頭蓋冠での骨形成の評価

    酒井進, 穴田貴久, 塩飽由香利, 濱井瞭, 土屋香織, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　40th　130 (WEB ONLY)　2018

  13. ハイブリッドスフェロイド培養法を用いたリン酸カルシウム系材料の骨再生能の評価

    佐藤智哉, 穴田貴久, 塩飽由香利, 濱井瞭, 土屋香織, 佐々木啓一, 鈴木治

    日本バイオマテリアル学会大会予稿集(Web)　40th　424 (WEB ONLY)　2018

  14. 未分化間葉系細胞とリン酸オクタカルシウムによる三次元複合組織体の構築

    佐藤 智哉, 穴田 貴久, 塩飽 由香利, 土屋 香織, 佐々木 啓一, 鈴木 治

    日本バイオマテリアル学会大会予稿集　39回　210-210　2017/11

    Publisher: 日本バイオマテリアル学会

  15. 種々のリン酸カルシウム系骨補填材料が骨細胞分化に与える影響

    蔡優広, 蔡優広, 塩飽由香利, 穴田貴久, 宮本洋一, 上條竜太郎, 高橋哲, 鈴木治

    再生医療　16　446　2017/02/01

    ISSN： 1347-7919

  16. The effect of three-dimensional culture on the ameloblastic differentiation of dental epithelial cells

    Takahisa Anada, Mayu Tadaki, Mayu Tadaki, Yukari Shiwaku, Yukari Shiwaku, Takashi Nakamura, Masanori Nakamura, Masaru Kojima, Tatsuo Arai, Satoshi Fukumoto, Osamu Suzuki

    2016 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2016　2017/01/18

    DOI： 10.1109/MHS.2016.7824173 　

  17. A-18 Evaluation of osteoconductivity of a spongy form of octacalcium phosphate/gelatin composites implanted in rabbit tibia bone defects

    Saito K, Anada T, Tsuchiya K, Chiba S, Itoi E, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　34　(5)　368-368　2015/09/15

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    The present study was designed to investigate whether osteoconductivity of octacalcium phosphate/gelatin (OCP/Gel) composites can be improved by controlling the content of OCP in the composites. The composites with some mixing ratios of OCP were prepared by mixing granules of OCP with Gel. OCP/Gel composites were implanted into the defect in rabbit tibia for 4 or 6 weeks and then examined radiographically, histologically, and histomorphometrically. The results indicated that the content of the OCP granules can control the osteoconductivity of OCP/Gel in the defect of rabit tibia.

  18. P-51 Analysis of bone regeneration in rat calvaria bone defect by octacalcium phosphate/ gelatin composites

    Sakai S, Anada T, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　34　(5)　395-395　2015/09/15

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Octacalcium phosphate co-precipitated gelatin composites (OCP/Gel) show superior osteoconductivity more than that of the original OCP. This study was designed whether the OCP/Gel composites, which were synthesized with some mixing ratios of OCP, promote bone regeneration in rat calvaria critical-sized bone defects. OCP/Gel composites were implanted for 8 weeks and then examined using 2D and 3D X-ray radiography. The results indicated that the osteoconductivity of OCP/Gel in the defect of rat calvaria could be controlled by the OCP inclusion in the composites.

  19. A-4 Influence of three-dimensional culture on differentiation of dental epithelial cells into ameloblasts

    Tadaki M, Anada T, Fukumoto S, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　34　(5)　316-316　2015/09/15

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Dental epithelial cell line SF2 cells secrete some enamel proteins stimulated by BMP-2 and NT-4. Three-dimensional cell culture can mimic natural condition in the body compared to monolayer culture. In this study, we investigated whether three dimensional culture affects the differentiation of SF2 cells into ameloblasts compared to the monolayer culture.

  20. Vascularized Bone Engineering using Rapid Vascular Fabrication Technique

    T. Kageyama, T. Osaki, J. Enomoto, T. Anada, O. Suzuki, J. Fukuda

    TISSUE ENGINEERING PART A　21　S406-S406　2015/09

    ISSN： 1937-3341

    eISSN： 1937-335X

  21. A-8 Bone regenerative property of a spongy form of octacalcium phosphate/gelatin composite in rabbit tibia bone defects

    Saito K, Anada T, Chiba S, Itoi E, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　34　(2)　65-65　2015/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    The present study was designed to investigate whether an octacalcium phosphate/gelatin (OCP/Gel) composite can repair a defect created in rabbit tibia within 4 and 6 weeks. Histological and μCT studies showed that the implantation of OCP/Gel composite enhanced cortical bone formation over the defect accompanied by the formation of cancellous bone within 6 weeks. The defects were not almost repaired in the control group (gelatin only) within 4 weeks. The repaired cortical bone by gelatin was thinner than that in the OCP/Gel-implanted group. The results suggest that an OCP/Gel composite could be effectively used as a bone substitute material.

  22. P-7 The formation of toroid multicellular aggregates by microfabrication technology

    Masuda T, Anada T, Suzuki O, Arai F

    The journal of the Japanese Society for Dental Materials and Devices　34　(2)　78-78　2015/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    We have developed a novel multicellular aggregate (spheroid) formation device that utilizes a maskless grey-scale photolithography. The device was capable of producing hundreds of aggregates rapidly at a time and regulating the diameter of aggregates with complex design. The cellular aggregates have a micro-pore to supply the growth factor and to expel the waste product. As a result, the cellular aggregates enable long-term culture. In addition we can use micro-pore as continuous channel and insert vascular system and nerve system into assembled tissue.

  23. A-1 Effect of mechanical stress on osteoblast-like cells seeded on the OCP/gelatin composites

    Yamada M, Anada T, Masuda T, Takano-Yamamoto T, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　34　(2)　57-57　2015/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    We have reported that OCP-gelatin (OCP/Gel) composites have a potential to enhance bone formation in various bone sites. However, certain mechanical stress suppresses the bone formation property of OCP under in vivo condition. Mechanical stimulation is considered to be one of the significant factors regulating the homeostasis, especially, skeletal structure and function. In the present study, we developed a cyclic mechanical loading device to evaluate the effect of mechanical stress on the osteoblastic differentiation of stem cells seeded on the three-dimensional scaffold. The effect of compression was analyzed using mouse mesenchymal stem cell line D1 cells by deforming the cell-seeded OCP/Gel composite. The results showed that osteoblastic differentiation of D1 cells was promoted if suitable conditions regarding mechanical stress were provided.

  24. P-52 Preparation of partially hydrolyzed octacalcium phosphate/gelatin composites and their cellular response

    Ezoe Y, Anada T, Kobayashi K, Handa T, Takahashi T, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　34　(2)　140-140　2015/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    We have previously shown that partially hydrolyzed octacalcium phosphate (OCP) has a better osteoconductivity more than that of the original OCP. This study was designed to investigate the effect of the presence of gelatin molecules on the hydrolysis reaction of OCP and the biological characteristics of OCP-co-precipitated gelatin composite (OCP/Gel) by partially hydrolysis reaction. The results showed that hydrolysis of OCP in OCP/Gel tended to advance more than the absence of Gel molecules in the hydrolysis reaction and the hydrolyzed OCP/Gel showed similar biocompatibility as the Gel alone.

  25. Interface oral health science 2014: Innovative research on biosis-abiosis intelligent interface

    Keiichi Sasaki, Suzuki Osamu, Nobuhiro Takahashi

    Interface Oral Health Science 2014: Innovative Research on Biosis-Abiosis Intelligent Interface　1-351　2015/01/01

    DOI： 10.1007/978-4-431-55192-8 　

    More details Close

    ? 2015, Springer Japan. All rights reserved. The Tohoku University Graduate School of Dentistry first introduced the concept of “Interface Oral Health Science”, designed to establish and maintain healthy oral cavities, which are home to a number of mixed systems. Included in those systems are: (1) host tissues such as teeth, mucosa, muscle and bone, (2) parasites and microorganisms cohabiting the surfaces of the oral cavity and (3) biomaterials that are used for the rehabilitation of oral functions. In addition, (4) these systems are subject to severe and complex mechanical forces. Therefore, it is critical to promote dental studies that integrate a wide range of interdisciplinary research as medicine, agriculture, material science, engineering, and pharmacology. With this incentive, international symposiums for interface oral health science have been held several times in the past. The concept has since refined and expanded, the result being the “Biosis-Abiosis Intelligent Interface,” and projects aiming at the creation of highly functional and autonomic intelligent interfaces are ongoing. This book brings together a number of studies on incentives and projects by leading authors. Topics include biosis-abiosis interface of dental implants, biomaterials in interface science, biomedical engineering interface and cell manipulation and tissue regeneration. Readers not only from the field of dentistry but also many related areas will find this book a valuable resource.

  26. P-72 The mechanobiological availability of Ti-Nb-Sn with low young's modulus and high-strength

    Takahashi K, Shiraishi N, Ishiko-Uzuka R, Anada T, Suzuki O, Masumoto H, Sasaki K

    The journal of the Japanese Society for Dental Materials and Devices　33　(5)　477-477　2014/09/20

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    It is reported that Ti-Nb-Sn has low Young's modulus possessing around 40 GPa close to human cortical bone (30 GPa) and low cytotoxicity as well as commercially pure Ti (cpTi). However, the availability for dental implant is unknown. The purpose of this study was to investigate the biomechanical behavior, biocompatibility and bioactivity of Ti-Nb-Sn. As a result, Ti-Nb-Sn is considered to have sufficient biological potential and capacity for osseointegration.

  27. A-11 Development of cell culture device to prevent oxygen deprivation of cellular aggregates

    Anada T, Kamoya T, Yamamoto T, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　33　(5)　382-382　2014/09/20

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    The multicellular structure of spheroid can be used as an in vitro model for the 3D cellular construct in tissue engineering. Since oxygen is one of the most important factors for cell survival and growth, oxygen deprivation (hypoxia) induces necrotic cell death in the center of the spheroid. We have developed an oxygen-permeable spheroid culture device using polydimethylsiloxane to prevent oxygen deprivation of the spheroid. In this study, we investigated the influence of oxygen supply on the cell growth, the viability, and the differentiation of hepatoma cell spheroid and mesenchymal stem cell spheroid.

  28. A-27 Extent of osteoconductivity of octacalcium phosphate with coexistence of amorphous calcium phosphate

    Kobayashi K, Anada T, Takahashi T, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　33　(5)　448-448　2014/09/20

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Octacalcium phosphate (OCP) and amorphous calcium phosphate (ACP) are both precursors in hydroxyapatite (HA) formation. They show osteoconduction by the implantation on mouse calvaria. ACP is known to be more soluble than OCR In the present study, we hypothesized that coexistence of ACP and OCP has an effect on controlling the conversion from OCP to HA, and therefore we analyzed the immersion in simulated body fluid (SBF) and the implantation into critical-sized rat calvaria defect in the mixed granules of ACP and OCP. The results suggest that the addition of ACP to OCP in granule forms may have a positive effect on the osteoconductivity of OCP.

  29. Hydrolysis of octacalcium phosphate and tissue response in bone

    32　(3)　180-187　2014/07

    Publisher: 日本バイオマテリアル学会 ; 2002-

    ISSN： 1347-7080

  30. ゼラチン/リン酸オクタカルシウム複合体の骨再生能・生体内吸収性に関する検討

    石河 理紗, 穴田 貴久, 佐々木 啓一, 鈴木 治

    日本補綴歯科学会誌　6　(特別号)　261-261　2014/05

    Publisher: (公社)日本補綴歯科学会

    ISSN： 1883-4426

    eISSN： 1883-6860

  31. A-17 Repair of rat calvaria critical-sized bone defect with gelatin/OCP composites

    Ishiko-Uzuka R, Anada T, Sasaki K, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　33　(2)　127-127　2014/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    In this study we have prepared a series of new gelatin/OCP complexes using OCP synthesized under specific conditions. After considering optimal material condition of gelatin/OCP composite, we evaluated the bone regeneration ability and bone maturity of the regenerative bone by using a rat calvaria critical-sized bone defect model. As a result, the utility as a bone substitute material of gelatin/OCP has been confirmed.

  32. P-52 Effects of oxygen supply on the differentiation of osteoblastic cells seeded in porous scaffolds

    Sawaki Y, Anada T, Kanari D, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　33　(2)　161-161　2014/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Bone regeneration using mesenchymal stem cells seeded in three-dimensional porous scaffolds is an attractive therapy in tissue engineering field. The present study was designed to develop and characterize an oxygen-permeable cell culture device and investigate whether the devise improves osteoblastic cell differentiation. Differentiation of mouse mesenchymal stem cells inoculated into the gelatin disks was examined. The results indicate that the device is capable of promoting the osteoblastic differentiation of mesenchymal stem cells seeded in porous scaffolds.

  33. 2F25 Osteoconductivity of octacalcium phosphate associated with its crystal characteristics

    SUZUKI Osamu, ANADA Takahisa

    2014　(26)　495-495　2014/01/10

    Publisher: The Japan Society of Mechanical Engineers

  34. 2F24 Development of a three-dimensional cell culture system and analysis of cell assemblies

    ANADA Takahisa, KAMOYA Takuo, SUZUKI Osamu

    2014　(26)　493-493　2014/01/10

    Publisher: The Japan Society of Mechanical Engineers

  35. 東北大学歯学部・歯学研究科における新機軸の教育研究の展開

    佐々木 啓一, 竹内 裕尚, 天雲 太一, 洪 光, 真柳 弦, 西岡 貴志, 鈴木 治, 高橋 信博, 小坂 健

    みちのく歯学会雑誌　44　(1-2)　17-21　2013/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  36. P-40 The effect of octacalcium phosphate (OCP) on the differentiation of dental epithelial cells

    Tadaki M, Anada T, Fukumoto S, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　32　(5)　390-390　2013/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Dental epithelial cell line SF2 expresses ameloblastin after stimulation of BMP2 or NT-4. Ameloblastin is one of the enamel matrices, and important for dental epithelial cell differentiation from iPS cells co-culturing with SF2 cells. Octacalcium phosphate (OCP) has been recognized to have a stimulatory capacity on osteoblastic differentiation. We evaluated whether OCP promotes ameloblastic cell differentiation.

  37. P-67 Evaluation of angiogenesis in bone regeneration by octacalcium phosphate/gelatin composites

    Murakami Y, Anada T, Shimauchi H, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　32　(5)　417-417　2013/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Octacalcium phosphate (OCP) has been recognized to have a stimulatory capacity on osteoblastic differentiation in vitro and stimulate bone formation in various bone defect models. Angiogenesis is a key component of bone regeneration. However, the mechanism of blood vessel development in the bone regeneration process by OCP remains unclear. In this study, we evaluated the angiogenesis in rat calvarial bone defects after the implantation of OCP gelatin composite.

  38. P-71 Surface modification of Ti-6Al-4V alloy by electron cyclotron resonance plasma oxidation

    Oikawa M, Masumoto H, Orii Y, Anada T, Sasaki K, Suzuki O

    The journal of the Japanese Society for Dental Materials and Devices　32　(5)　421-421　2013/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Ti-6Al-4V alloy is used as biomaterials for dental and orthopedic implants because of their excellent biocompatibilities and mechanical properties. However, it is unclear that surface modification techniques improve osseointegration of the alloy. Electron cyclotron resonance (ECR) plasma is high-active plasma, and it is suitable for generating highly crystalline films at low temperatures. The purpose of this study was to investigate the characteristics and the biocalcification ability of the oxidation films created by ECR plasma oxidation apparatus on the Ti-6Al-4V alloy. The results suggested that altered surface of Ti-6Al-4V alloy by ECR plasma oxidation can improve the biocalcification ability.

  39. Octacalcium phosphate (OCP)-based bone substitute materials

    Osamu Suzuki

    Japanese Dental Science Review　49　(2)　58-71　2013/05

    DOI： 10.1016/j.jdsr.2013.01.001 　

    ISSN： 1882-7616

  40. Characterization of bone substitute materials gelatin and gelatin/OCP complex

    UZUKA R, ANADA T, KANDA N, KOBAYASHI K, SASAKI K, SUZUKI O

    The journal of the Japanese Society for Dental Materials and Devices　32　(2)　107-107　2013/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    We previously reported that complex consisted of gelatin and octacalcium phosphate (OCP) is a highly osteoconductive material. The purpose of this study was to examine the optimal material condition of gelatin/OCP complex to reveal better performance as bone substitute material. Gelatin samples, made up from 0.5 to 3.0wt% gelatin concentration in preparation, were subjected to the material characterization and in vivo tests. The biocompatibility and bone regeneration of the gelatin were influenced by the preparation condition of the gelatin. Gelatin/OCP complexes were prepared in the range of these gelatin concentrations and further analyzed from the view point of the material and biocompatible properties.

  41. Preparation of alginate/OCP beads as a new bone substitute material and analysis of osteoblastic cell viability

    ENDO K, ANADA T, YAMADA M, SEKI M, SASAKI K, SUZUKI O

    The journal of the Japanese Society for Dental Materials and Devices　32　(2)　104-104　2013/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    The aim of this study was to develop a new bone substitute using alginate hydrogels and octacalcium phosphate (OCP) for minimally invasive surgery. Alginate hydrogels are suitable for containing live cells without damages. OCP enhances bone mineralization. Our hypothesis is that the alginate/OCP beads can be used as a new bone substitute material. In this study, we report a method to prepare alginate beads and the viability of ST2, a mouse bone marrow-derived stromal cell line, in alginate beads.

  42. A study on in vitro cellular compatibility of synthetic octacalcium phosphate

    MORIMOTO S, ANADA T, HONDA Y, SUZUKI O

    The journal of the Japanese Society for Dental Materials and Devices　31　(5)　447-447　2012/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Octacalcium phosphate (OCP) is a biodegradable ceramic material which is known to show better osteoconductivity. The present study was designed to investigate the extent to which OCP displays cytotoxic and inflammatory responses based on its dissolution in vitro by comparing with other calcium phosphate ceramics. Cell proliferation on the sample coated plates, estimation of calcium and phosphate concentration, and degree of supersaturation in the medium supernatant, and cytotoxicity test and inflammatory response test were performed. The results obtained indicate that OCP is a biocompatible material as well as hydroxyapatite and β-tricalcium phosphate which are already used clinically.

  43. Development of cyclic mechanical loading device for 3D scaffold

    YAMADA M, MASUDA T, ANADA T, HONDA Y, YAMAMOTO TAKANO T, SUZUKI O

    The journal of the Japanese Society for Dental Materials and Devices　31　(5)　402-402　2012/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Mechanical stimulation is considered to be one of factors regulating the homeostasis of bone, especially, the skeletal structure and the function. However, little is known about the mechanism of cell sensing stimulation. Although many studies have reported the effect of mechanical stress on monolayered cells attached on plate, the effect has not sufficiently been examined in 3D cultured cells so far. We have developed a cyclic uni-axial mechanical loading device for 3D scaffold including cells. In the present study, we analyzed the effect of the mechanical stress on osteoblastic cell growth within 3D gelatin scaffold in vitro.

  44. The effect of amorphous calcium phosphate films by radiofrequency magnetron sputtering on TNTZ implants on integration with bone

    SHIRAISHI N, NARUSHIMA T, GOTO T, NIINOMI M, SUZUKI O, SASAKI K

    The journal of the Japanese Society for Dental Materials and Devices　31　(5)　385-385　2012/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Ti-29Nb-13Ta-4.6Zr alloy (TNTZ) has excellent advantages as a biomaterial, such as low Young's modulus, low cytotoxicity, and the absence of allergens. However, it is unclear whether TNTZ can achieve sufficient osseointegration as a dental implant. The effectiveness of surface modification of TNTZ implants with amorphous calcium phosphate (ACP) by radiofrequency (RF) magnetron sputtering is also unclear. We investigated the biomechanical behaviors of TNTZ implants in vivo. The results suggest that TNTZ implants can achieve osseointegration similar to that of pure Ti, and that surface modification with ACP by RF magnetron sputtering improves osseointegration.

  45. Alveolar augmentation by octacalcium phosphate collagen composites with titanium mesh in canine mandibular bone defects

    K. Miura, K. Matsui, T. Kawai, Y. Kato, A. Matsui, O. Suzuki, S. Kamakura, S. Echigo

    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE　6　2-2　2012/09

    ISSN： 1932-6254

  46. Effect of dehydration on osteoconductive capacity of octacalcium phosphate bone substitute material

    SHINTANI S, ANADA T, HONDA Y, KAWAI T, SHIMAUCHI H, SUZUKI O

    The journal of the Japanese Society for Dental Materials and Devices　31　(2)　106-106　2012/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    We have shown that OCP is a biodegradable and highly osteoconductive material and that its capacity is improved if partially hydrolyzed in water. OCP can be decomposed by heating in the air due to water molecule dehydration. In this study, we examined the bone regeneration of partially dehydrated OCP if implanted onto calvarial bone of rats in a granule form. There is a report that the dehydration produces similar portion in the structure produced by the partial hydrolysis. It was apparent that the partial dehydration of OCP influences the bone regeneration enhanced by OCP.

  47. Effect of introduction of amorphous calcium phosphate on osteoconductivity of octacalcium phosphate bone substitute material

    KOBAYASHI K, ANADA T, HONDA Y, HANDA T, KAMAKURA S, ECHIGO S, SUZUKI O

    The journal of the Japanese Society for Dental Materials and Devices　31　(2)　94-94　2012/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Octacalcium phosphate (OCP) is an osteoconductive material, the property of which could be induced by a conversion process from OCP to hydroxyapatite (HA). The present study was designed to investigate whether addition of amorphous calcium phosphate (ACP) into OCP in granule forms has an effect on raising the intrinsic osteoconductivity of OCP if implanted into critical sized rat calvaria defect. ACP is known to be more soluble than OCP so that the OCP conversion would be controlled. Radiographic and histological studies of the implants showed that the introduction of ACP may have a positive effect on bone regeneration with OCP.

  48. Interface oral health science 2011

    Keiichi Sasaki, Osamu Suzuki, Nobuhiro Takahashi, Philip Stashenko, Thomas E. Van Dyke, Toshihisa Kawai, Hatice Hasturk, Alpdogan Kantarci

    Interface Oral Health Science 2011　1-422　2012/01/01

    DOI： 10.1007/978-4-431-54070-0 　

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    ? Springer 2012. Interface oral health science was founded on the concept that healthy oral function is maintained by biological and biomechanical harmony between three systems: oral tissues, parasitic oral microorganisms, and biomaterials. On that basis, dental caries, periodontal disease, and temporomandibular joint disorders may be regarded as interface disorders that result from a disruption in the intact interface of these systems. Interface oral health science encompasses the fields of dentistry and dental medicine, but also extends to general medicine, agriculture, biomaterials science, bioengineering, and pharmacology. This book is a compendium of the research presented at symposiums held in 2011 by the Tohoku University Graduate School of Dentistry and by the Forsyth Institute. Its publication is intended provide further impetus for the progress of oral science and health, pointing the way for dental research for future generations.

  49. Synthesis of octacalcium phosphate with fluoride ions and protein adsorption onto the crystals

    Yukari Shiwaku, Takahisa Anada, Yoshitomo Honda, Shinji Morimoto, Keiichi Sasaki, Osamu Suzuki

    Interface Oral Health Science 2011　237-239　2012/01

    DOI： 10.1007/978-4-431-54070-0_67 　

  50. Effect of basic-fibroblast growth factor on osteogenic differentiation of rat mesenchymal stem cells

    T. Kawai, T. Anada, T. Masuda, Y. Honda, Y. Sakai, Y. Kato, S. Echigo, O. Suzuki

    24th European Conference on Biomaterials - Annual Conference of the European Society for Biomaterials　2011/12/01

  51. Surface modification of porous titanium having young's modulus similar to human cortical bone by electron cyclotron resonance plasma oxidation

    M. Fujikawa, H. Masumoto, M. Oikawa, Y. Orii, N. Nomura, O. Suzuki, K. Sasaki

    24th European Conference on Biomaterials - Annual Conference of the European Society for Biomaterials　2011/12/01

  52. System engineering approach for identifying the optimal BMP2-included osteogenic drug cocktails

    HONDA Y, SUZUKI O, NISHIMURA I

    The journal of the Japanese Society for Dental Materials and Devices　30　(5)　285-285　2011/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Bone morphogenic protein 2 (BMP2) is known to be a potent osteogenic factor. However, supraphysiological concentrations are required to achieve its therapeutic effect, resulting in higher healthcare cost and significant side effects that may prevent its routine clinical use. Although drug combinations offer a promising alternative to single drug administration, the identification of optimal drug combinations is extremely challenging due to the large number of possible combinations. In this presentation, we will present the novel feedback system control (FSC) approach, to identify the optimal osteogenic drug cocktails containing minimal BMP2 concentrations.

  53. Characterization of octacalcium phosphate/gelatin composites

    ANADA T, HANDA T, YAMAZAKI H, HONDA Y, KANDA N, ECHIGO S, SUZUKI O

    The journal of the Japanese Society for Dental Materials and Devices　30　(5)　351-351　2011/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    The present study was designed to synthesize and characterize octacalcium phosphate (OCP)-precipitated gelatin (Gel) composites and investigate whether the composites improve osteoblastic cell affinity. OCP crystals were grown with various concentrations of Gel molecules. OCP/Gel composites were characterized by chemical analysis, x-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), and mercury intrusion porosimetry. Proliferation of mouse bone marrow stromal ST-2 cells, inoculated into the disks of OCP/Gel composite, was examined. The results indicate that osteoblastic cells can easily be invaded into OCP/Gel composite.

  54. メカニカルストレスによる骨芽細胞シグナル伝達路活性化メカニズムの分子生物学的解析

    福野 直人, 松井 裕之, 神田 佳明, 鈴木 治, 小林 孝安, 田村 眞理, 佐々木 啓一

    日本補綴歯科学会誌　3　(特別号)　314-314　2011/05

    Publisher: (公社)日本補綴歯科学会

    ISSN： 1883-4426

  55. 骨芽細胞のJNKおよびp38は骨メカノバイオロジーにおいて破骨細胞とのサイトカインクロストークに寄与する

    松井 裕之, 福野 直人, 神田 佳明, 鈴木 治, 武田 弘資, 一條 秀範, 佐々木 啓一, 小林 孝安, 田村 眞理

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　83回・33回　1T2-9　2010/12

    Publisher: (公社)日本生化学会

  56. Osteoconductivity of Octacalcium Phosphate : Physicochemical Property and Bone Tissue Reaction

    SUZUKI Osamu

    The journal of the Japanese Society for Dental Materials and Devices　29　(5)　449-449　2010/09/20

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

  57. New Inorganic-organic hybrid scaffolds for bone regeneration

    SHIRAISHI N, ANADA T, HONDA Y, SASAKI K, SUZUKI O

    The journal of the Japanese Society for Dental Materials and Devices　29　(5)　458-458　2010/09/20

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    The aim of this study is to fabricate the new inorganic-organic hybrid scaffolds for bone regeneration. The conditions required are osteoconductivity for inorganic material and biocompatibility, biodegradability, low immunogenicity, optimum mechanical strength and pore formation for organic material. For this purposes, we selected the β-TCP and gluten. The complex was mixed with water and then burned to prepare 6 types of Gluten/β-TCP scaffolds. The property of the scaffold, including the pore size and the mechanical strength was investigated.

  58. BSA and cytochrome c adsorption behaviors on octacalcium phosphate prepared in the presence of fluoride

    SHIWAKU Y, HONDA Y, ANADA T, MORIMOTO S, SASAKI K, SUZUKI O

    The journal of the Japanese Society for Dental Materials and Devices　29　(5)　386-386　2010/09/20

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Octacalcium phosphate (OCP) has been suggested to be a precursor of biological apatite and proved to exhibit osteoconductive and biodegradable properties. We previously reported that hydrolytic conversion from OCP to apatite plays a role in enhancing osteoblastic cell differentiation. Fluoride ion is known not only to promote OCP-apatite conversion but also to modify protein adsorption onto calcium phosphate. The aim of this study was to investigate the effect of synthesis conditions on protein adsorption onto fluoride-containing calcium phosphate (F-CaP) which is derived from OCP. The results showed that F-CaPs have different protein adsorption capacities depending on the synthesis conditions.

  59. リン酸オクタカルシウムによる未分化軟骨細胞の分化抑制の機序

    澁谷勲, 宮本洋一, 吉村健太郎, 鈴木治, 上條竜太郎

    日本整形外科学会雑誌　84　(8)　S1324-S1324　2010/08/25

    Publisher: (公社)日本整形外科学会

    ISSN： 0021-5325

  60. Bonding Strength of the Hydroxyapatite Film on Tooth Using Powder-Jet-Deposition

    AKATSUKA R, NOJI M, SEPASY ZAHMATY M. S., ANADA T, OGAWA K, KURIYAGAWA T, SUZUKI O, SASAKI K

    1　(118)　112-112　2010/08/16

  61. 市販β-TCP焼結体と比較した合成リン酸オクタカルシウム (OCP) の骨芽細胞の分化能 (日本口腔外科学会雑誌)

    川井忠, 穴田貴久, 本田義知, 鎌倉慎治, 松井有恒, 松井桂子, 越後成志, 鈴木治

    日本口腔外科学会雑誌　56　(1)　2-8　2010/04

    Publisher: Japanese Society of Oral and Maxillofacial Surgeons

    DOI： 10.5794/jjoms.56.2 　

    ISSN： 0021-5163

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    Octacalcium phosphate (OCP) has been suggested to be a precursor of biologic apatite crystals in bones and teeth. Previous studies have shown that synthetic OCP facilitates osteoblastic cell differentiation in vitro. The present study was designed to investigate whether OCP facilitates osteoblastic cell differentiation in vitro as compared with commercially available sintered β-tricalcium phosphate (β-TCP) ceramic, a bone regenerative and biodegradable material used clinically. Mouse-bone-marrow-derived stromal ST-2 cells were cultured on dishes pre-coated with powdered OCP or commercially available sintered β-TCP ceramic. The capacity for proliferation and differentiation was determined up to day 21 of culture. The proliferation of ST-2 cells on OCP coating was inhibited intially, but the cell number increased similar to that of commercially available sintered βTCP ceramic or control culture dishes up to day 14. On the other hand, the alkaline phosphatase activity of ST-2 cells on the OCP coating, a maker of osteoblastic differentiation, was higher than that of commercially available sintered β-TCP ceramic or control up to day 14. Measurement of the mRNA expression level of osteopontin (OPN) by real-time PCR on day 21 of culture showed that the OPN mRNA level of ST-2 cells on OCP coating was higher than that of cells on commercially available sintered β-TCP ceramic. Chemical analysis of the supernatants of the medium after incubation on the coatings of OCP and commercially available sintered β-TCP ceramic revealed distinct patterns: commercially available sintered β-TCP ceramic decreased both Ca2＋ and inorganic phosphate ion (Pi) concentrations, whereas OCP decreased Ca2＋ but increased Pi ion concentrations beyond the control. These changes may be derived from the intrinsic physicochemical properties of OCP. The present study demonstrated that OCP facilitates osteoblastic cell differentiation more than that associated with commercially available sintered β-TCP ceramic.

  62. Bone regeneration by octacalcium phosphate (OCP) collagen composites (Bone Regeneration: Growth Factors, Augmentation Procedures and Tissue Engineering Applications, Chapter 6, Eds V Legard and R Schluter, Nova Science Publisher, NY)

    S Kamakura T, Anada O Suzuki

    Bone Regeneration: Growth Factors, Augmentation Procedures and Tissue Engineering Applications, Chapter 6, Eds V Legard and R Schluter, Nova Science Publisher, NY　177-202　2010/04

  63. Synthesis and characterization of octacalcium phosphate prepared by hydrolysis and co-precipitation in the presence of fluoride

    SHIWAKU Y, HONDA Y, ANADA T, MORIMOTO S, SASAKI K, SUZUKI O

    The journal of the Japanese Society for Dental Materials and Devices　29　(2)　144-144　2010/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 1884-4421

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    Fluoridated hydroxyapatite (FHA) is known to stimulate osteoblast function and alter protein adsorption behavior depending on the degree of fluoridation of HA. Because octacalcium phosphate (OCP) has a structural similarity to HA, we hypothesized that the interaction between fluoride ion and OCP possibly improves the osteogenic capability of original OCP. In this study, we investigated the synthesis and characterization of OCP prepared by hydrolysis and co-precipitation methods in the presence of fluoride. The results showed that the products prepared by different methods have district physicochemical properties, such as crystal structure, solubility and protein adsorption.

  64. Molecular mechanisms of the response to mechanical stimulation during chondrocyte differentiation

    Takahashi I, Masuda T, Kohsaka K, Terao F, Anada T, Sasano Y, Takano-Yamamoto T, Suzuki O

    Interface Oral Health Science 2009　2010

  65. Biological role of synthetic octacalcium phosphate in bone formation and mineralization

    Osamu Suzuki

    Journal of Oral Biosciences　52　(1)　6-14　2010

    Publisher: Japanese Association for Oral Biology

    DOI： 10.2330/joralbiosci.52.6 　

    ISSN： 1349-0079

  66. 高分子リンカーを有するリン酸カルシウム結合性リポソームの機能評価

    穴田貴久, 本田義知, 武田陽一, 櫻井和朗, 鈴木治

    日本バイオマテリアル学会大会予稿集　31st　394　2009/11/16

  67. Effect of amino acids on Zn release from Zn-incorporated octacalcium phosphate and hydroxyapatite

    HONDA Y, ANADA T, SUZUKI O

    Journal of the Japanese Society for Dental Materials and Devices　28　(5)　235-235　2009/09/10

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

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    Elucidation of Zinc-released mechanism from Zinc incorporated calcium phosphate (Zn-CaP) is fundamental to develop safe and effective Zn-CaPs in the biomedical use. However, little attention has been given to the involvement of amino acids in regulating Zn release. In the present study, we provide evidence that some of amino acids, such as cysteine and histidine, facilitate markedly the Zn release from the Zn-incorporated octacalcium phosphate and hydroxyapatite.

  68. Preparation and characterization of porous alginate/octacalcium phosphate scaffolas

    SHIRAISHI N, ANADA T, HONDA Y, MASUDA T, SASAKI K, SUZUKI O

    Journal of the Japanese Society for Dental Materials and Devices　28　(5)　271-271　2009/09/10

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

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    We previously fabricated alginate(Alg) / octacalcium phosphate(OCP) scaffolds by co-precipitation method (OCP-precipitated Alg), and demonstrated that increasing pore size improved cell attachment in vitro and bone regeneration in vivo. The present study was designed to investigate whether the amount of OCP affects the material characteristics, such as the pore size, when the granules of OCP are mixed in Alg. The results showed that immersion of Alg/OCP in SBF promoted OCP conversion and apatite deposition, suggesting that the Alg/OCP may have osteoconductive nature. The pore size of the composites was dependent on the amount of OCP.

  69. P-53 リン酸オクタカルシウム(OCP)に集積するラット血清由来タンパク質のプロテオーム解析(生体組織・毒性,一般講演(ポスター発表),第54回日本歯科理工学会学術講演会)

    金子裕史, 上家潤一, 穴田貴久, 本田義知, 鎌倉慎治, 寺崎哲也, 島内英俊, 鈴木治

    歯科材料・器械　28　(5)　2009/09/10

    Publisher: 日本歯科理工学会

    ISSN： 0286-5858

    More details Close

    Previous study showed that octacalcium Phosphate (OCP) enhances bone formation and accumulation of circulating serum proteins on its surface if implanted in bone defect. In this study, serum proteins accumulated on OCP and hydroxyapatite (HA) in vitro were analyzed by proteomic approaches using liquid chromatography-tandem mass spectrometry (LC-MS/MS). 139 proteins were detected from OCP surface in total. 46 of them were indentical to the proteins accumulated on HA. The difference in the proteins found could be a potential factor to display the distinct osteoconductive nature between OCP an...

  70. Chondroitin sulfate-e enhances osteoblast differentiation and inhibits osteoclast differentiation in vitro

    T. Miyazaki, S. Miyauchi, A. Tawada, T. Anada, S. Matsuzaka, O. Suzuki

    BONE　44　(2)　S314-S314　2009/06

    DOI： 10.1016/j.bone.2009.03.587 　

    ISSN： 8756-3282

  71. Transitory nature of octacalcium phosphate in physiological milieu and its osteoconductive property (Phosphorus Research Bulletin )

    Suzuki O

    Phosphorus Research Bulletin　23　31-34　2009/04

    DOI： 10.3363/prb.23.31 　

  72. 生体に働きかけるバイオマテリアルの開発をめざして (J Dent Eng (DE) )

    鈴木治, 穴田貴久, 本田義知

    J Dent Eng (DE)　170　33-35　2009/04

  73. Synergistic immunostimulating effect of CpG DNA/calcium phosphate complex

    HONDA Y, ANADA T, SUZUKI O

    Journal of the Japanese Society for Dental Materials and Devices　28　(2)　115-115　2009/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  74. Development of calcium phosphate-binding liposomes

    ANADA T, HONDA Y, SUZUKI O

    Journal of the Japanese Society for Dental Materials and Devices　28　(2)　129-129　2009/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  75. Effect of stoichiometry of synthetic octacalcium phosphate (OCP) on its bone regenerative property

    MIYATAKE N., KISHIMOTO KN., ANADA T., IMAIZUM H., ITOI E., SUZUKI O.

    Journal of the Japanese Society for Dental Materials and Devices　28　(2)　86-86　2009/03/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  76. S108 Biocompatibility of biodegradable calcium phosphate bone substitute

    SUZUKI Osamu, ANADA Takahisa

    2008　(21)　329-330　2009/01/22

    Publisher: The Japan Society of Mechanical Engineers

  77. Molecular mechanisms of mechanical stress response during chondrogenesis

    Ichiro Takahashi, Taisuke Masuda, Kumiko Kohsaka, Fumie Terao, Takahisa Anada, Yasuyuki Sasano, Teruko Takano-Yamamoto, Osamu Suzuki

    Journal of Biomechanical Science and Engineering　4　(3)　307-317　2009

    DOI： 10.1299/jbse.4.307 　

    ISSN： 1880-9863

  78. リン酸カルシウムに特異的に結合するリポソームの合成と応用

    穴田貴久, 武田陽一, 櫻井和朗, 鈴木治

    日本バイオマテリアル学会シンポジウム予稿集　2008　294　2008/11/17

  79. Effect of octacalcium phosphate combined with collagen on subperiosteal bone augmentation

    MATSUI A., ANADA T., MASUDA T., KAMAKURA S., ECHIGO S., SUZUKI O.

    Journal of the Japanese Society for Dental Materials and Devices　27　(5)　355-355　2008/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  80. 局所環境が硬組織形成細胞の分化に及ぼす影響について (生体医工学)

    鈴木治, 益田泰輔, 高橋一郎

    生体医工学　46　414-418　2008/07

    DOI： 10.11239/jsmbe.46.414 　

  81. リン酸オクタカルシウムの骨再生能と生体内吸収性 (セラミックス)

    鈴木治

    セラミックス　43　286-289　2008/07

  82. 骨置換性材料“リン酸オクタカルシウム” (バイオマテリアル？生体材料)

    鈴木 治, 鎌倉慎治, 穴田貴久

    バイオマテリアル？生体材料　26　(3)　199-205　2008/07

  83. 生体模倣材料による生理的骨再生へのアプローチ (再生医療)

    鈴木 治, 鎌倉慎治, 穴田貴久本田義知

    再生医療　7　131-136　2008/07

  84. Molecular Biological Analysis of the Response to Mechanical Stress in Mouse Pre-osteoblastic Cell

    MATSUI H, FUKUNO N, KOBAYASHI T, SUZUKI O, TAMURA S, SASAKI K

    52　(117)　128-128　2008/06/06

  85. Bone Augmentation by Implantation of Octacalcium Phosphate Combined with Collagen (OCP/Col) in Subperiosteal Region

    SUZUKI Y, KAMAKURA S, HATORI K, HONDA Y, ANADA T, SUZUKI O, SASAKI K

    52　(117)　125-125　2008/06/06

  86. A-24 Octacalcium phosphate (OCP)/sodium alginate complex as a bone substitute material

    FUJI T., ANADA T., HONDA Y., SASAKI K., SUZUKI O.

    Journal of the Japanese Society for Dental Materials and Devices　27　(2)　193-193　2008/03/31

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  87. Bone regeneration by synthetic octacalcium phosphate and its role in biological mineralization

    O. Suzuki, H. Imaizumi, S. Kamakura, T. Katagiri

    Current Medicinal Chemistry　15　(3)　305-313　2008/02

    DOI： 10.2174/092986708783497283 　

    ISSN： 0929-8673

  88. (Regenerative medicine)

    鈴木治, 鎌倉慎治, 穴田貴久, 本田義知

    Regenerative medicine　7　131-136　2008

  89. Biodegradable synthetic octacalcium phosphate bone substitute (Environmental Biodegradation Research (Nova Science Publishers, Inc., Hauppauge, NY))

    Takahisa Anada, Hideki Imaizumi, Shinji Kamakura, Osamu Suzuki

    Environmental Biodegradation Research (Nova Science Publishers, Inc., Hauppauge, NY)　in press　2007/12

  90. 下顎器官培養系におけるエレクトロポレーション法による遺伝子導入の応用

    寺尾 文恵, 高橋 一郎, 三谷 英稔, 春山 直人, 鈴木 治, 笹野 泰之, 山本 照子

    日本矯正歯科学会大会プログラム・抄録集　66回　135-135　2007/09

    Publisher: (公社)日本矯正歯科学会

  91. Titanium Oxide Film Formation by Electron Cyclotron Resonance Plasma Oxidation and Its Calcifocation Ability

    ORII Y, MASUMOTO H, SUZUKI O, HONDA Y, ANADA T, GOTO T, SASAKI K

    51　(116)　110-110　2007/05/18

  92. Development of mechanical strain cell culture system for mechanobiological analysis

    Masuda T, Takahashi, I, Matsui A, Anada T, Arai F, Takano-Yamamoto-T, Suzuki O

    Proceeding, Interface Oral Health Science　167　2007

  93. Mechanical stretch inhibits chondrogenesis through ERK1/2 phosphorylation in micromass culture

    Takahashi I Terao, F, Masuda T, Sasano Y, Suzuki, O, Takano-Yamamoto T

    Proceeding, Interface Oral Health Science　161　2007

  94. 4.骨再生材料OCPはマウス骨髄由来間質細胞の骨芽細胞への分化を促進する(第49回東北大学歯学会講演抄録,歯学情報)

    塩飽 由香利, 尾崎 尚, 鍵和田 沙紋, 本田 義知, 熊谷 崇, 穴田 貴久, 鎌倉 慎治, 鈴木 治, 東北大学歯学部, 東北大学歯学部, 東北大学歯学部, 東北大学大学院歯学研究科顎口腔機能創建分野, 口腔生物学講座歯内歯周治療学分野, 東北大学大学院歯学研究科顎口腔機能創建分野, 東北大学大学院医学系研究科創生応用医学研究センター, 東北大学大学院歯学研究科顎口腔機能創建分野

    東北大学歯学雑誌 = Tohoku University dental journal　25　(2)　87-88　2006/12/01

    ISSN： 0287-3915

  95. リン酸オクタカルシウムの骨伝導能を応用した骨再生 (上原記念生命科学財団研究報告集)

    鈴木治

    上原記念生命科学財団研究報告集　20　359-362　2006/10/01

  96. Proliferation and differentiation of osteoblastic cells on octacalcium phosphate

    ANADA T., HONDA Y., KAMAKURA S., SUZUKI O.

    Journal of the Japanese Society for Dental Materials and Devices　25　(5)　350-350　2006/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  97. Mechanism of osteoclast differentiation induced by octacalcium phosphate

    MOCHIZUKI A, TAKAMI M, KAWAWA T, SUZUKI O, NAKAMURA M, KAMIJO R

    Journal of the Japanese Society for Dental Materials and Devices　25　(5)　333-333　2006/09/25

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  98. Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line (vol 345, pg 1155, 2006)

    Yoshitomo Honda, Takahisa Anada, Shinji Kamakura, Masanori Nakamura, Shunji Sugawara, Osamu Suzuki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　347　(3)　843-843　2006/09

    DOI： 10.1016/j.bbrc.2006.06.124 　

    ISSN： 0006-291X

  99. 組織置換型骨再生材料の開発 (．日本歯科産業学会会報（ニュースレター）)

    鈴木治

    ．日本歯科産業学会会報（ニュースレター）　34　(9)　1-2　2006/09/01

  100. 生体模倣材料を用いてどこまで生理的な骨再生機序に近づけるか (バイオマテリアル「生体材料」)

    鈴木治

    バイオマテリアル「生体材料」　24　(4)　361-362　2006/07/01

  101. 培養歯根膜細胞のバイオミネラル基質に対する生物学的対応

    畠山 純子, 畠山 雄次, 鈴木 治, 笹野 泰之

    解剖学雑誌　81　(2)　70-70　2006/06

    Publisher: (一社)日本解剖学会

    ISSN： 0022-7722

  102. The fabrication and evaluation of calcified OCP/collagen complex

    HONDA Y., KAMAKURA S., SUZUKI O.

    Journal of the Japanese Society for Dental Materials and Devices　25　(2)　103-103　2006/04/05

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  103. Enhancement of bone regeneration by the implantation of OCP-Collagen composite

    KAMAKURA S., HONDA Y., ANADA T., SUZUKI O.

    Journal of the Japanese Society for Dental Materials and Devices　25　(2)　139-139　2006/04/05

    Publisher: The Japanese Society for Dental Materials and Devices (JSDMD)

    ISSN： 0286-5858

  104. Surface chemistry and biological responses to synthetic octacalcium phosphate

    O Suzuki, S Kamakura, T Katagiri

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS　77B　(1)　201-212　2006/04

    DOI： 10.1002/jbm.b.30407 　

    ISSN： 1552-4973

  105. 合成バイオミネラル上の歯根膜細胞の接着と増殖

    畠山 純子, 畠山 雄次, 高橋 一郎, 鈴木 治, 笹野 泰之

    Journal of Oral Biosciences　47　(Suppl.)　105-105　2005/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  106. Proliferation and adhesion of periodontal ligament cells on synthetic biominerals

    Junko Hatakeyama, Yuji Hatakeyama, Ichiro Takahashi, Osamu Suzuki, Yasuyuki Sasano

    International Congress Series　1284　328-329　2005/09

    DOI： 10.1016/j.ics.2005.06.089 　

    ISSN： 0531-5131

  107. Interface of synthetic inorganic biomaterials and bone regeneration

    Osamu Suzuki

    International Congress Series　1284　274-283　2005/09

    DOI： 10.1016/j.ics.2005.06.066 　

    ISSN： 0531-5131

  108. 硬組織代替用生体材料と生体組織のインターフェイス (防菌防黴)

    鈴木 治, 鎌倉慎治

    防菌防黴　33　215-223　2005/04

  109. Assessing the particulate containment performance of pharmaceutical equipment (ISPE Good Practice Guide)

    Gurney-Read P, Koch M, Suzuki O, Heidel D, Fluckiger A, Abromovitz M, Wren J, Harrison D, Sargent E, Marshall P, Boland F, Buechl S, Farris J, Wilkins J, Ainsworth D, Fukushima Y, Meiners M, Kaplan JI, Floura H, Drew D, Zuidar A, Lunardi V, Cocker N

    ISPE Good Practice Guide　1-78　2005/04/01

  110. ラット臼歯胚移植後の象牙質形成と石灰化の動態について

    小田島 奈美, 笹野 泰之, 鈴木 治, 佐々木 啓一

    Journal of oral biosciences　46　(5)　377-377　2004/09/01

    Publisher: 歯科基礎医学会

    ISSN： 1349-0079

  111. オクタリン酸カルシウムの構造変化が骨形成に重要な役割を果たす

    鈴木 治, 片桐 岳信, 上條 竜太郎, 中村 雅典, 鎌倉 慎治, 沼田 守

    Journal of oral biosciences　46　(5)　2004/09/01

    Publisher: 歯科基礎医学会

    ISSN： 1349-0079

  112. [Interface science in formation of biominerals and biomolecules] (Clin Calcium)

    Suzuki Osamu

    Clin Calcium　14　(6)　9-15　2004/06

  113. 硬組織ミネラルの科学―バイオミネラルと生体成分のインターフェイス― (CLINICAL CALCIUM )

    鈴木治

    CLINICAL CALCIUM　14　9-15　2004/04

  114. バイオマテリアル・インターフェイス研究の新しい展開 (東北大歯誌)

    鈴木治

    東北大歯誌　23　60-66　2004/04

  115. Adsorption and fouling at stainless steel surfaces (Encyclopedia of Surface and Colloid Science)

    Suzuki O

    Encyclopedia of Surface and Colloid Science　Encyclopedia　167-174　2002/04

  116. 燐酸オクタカルシウムが促す骨修復のpQCTを用いた定量系の確立 : ラット頭頂骨部分欠損モデルの alive での解析

    中條 悟, 笹野 泰之, 鎌倉 慎治, 鈴木 治, 加賀山 学, 国分 正一

    日本骨代謝学会雑誌 = Japanese journal of bone metabolism　16　(2)　61-61　1998/07/07

    ISSN： 0910-0067

  117. 燐酸オクタカルシウムを用いた歯槽堤増大に関する実験的研究

    鎌倉 慎治, 茂木克俊, 笹野泰之, 中村雅典, 田辺 元, 鈴木 治

    日本口腔外科学会雑誌　38　(13)　2042-2042　1992/12

    Publisher: (公社)日本口腔外科学会

    ISSN： 0021-5163

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Books and Other Publications 19

  1. 人工骨材料 (リン酸八カルシウム材料)（人工臓器 51:160-164）

    鈴木 治,井樋栄二

    学研メディカル秀潤社・日本人工臓器学会　2022/12

  2. Novel scaffold composites containing octacalcium phosphate (Octacalcium Phosphate Biomaterials, pp.121-145. Edited by O Suzuki, G Insley)

    2019/11

  3. Octacalcium phosphate effects on the systemic and local factors that regulate bone-cell activity (Edited by O Suzuki, G Insley)

    2019/11

  4. Evolution of octacalcium phosphate biomaterials (Octacalcium Phosphate Biomaterials, pp.1-15. Edited by O Suzuki, G Insley)

    Osamu Suzuki

    2019/11

  5. Octacalcium Phosphate Biomaterials

    Osamu Suzuki, Gerard Insley

    Elsevier Ltd., Woodhead Publishing　2019/11

  6. リン酸八カルシウム足場材料の開発とその応用（再生医療の開発戦略と最新研究事例集, pp.141-160）

    鈴木 治, 濱井 瞭

    情報技術協会　2019

  7. 3次元オルガノイド形成（歯科再生医学, pp.206-215）

    穴田貴久, 鈴木 治

    医歯薬出版株式会社　2019

  8. リン酸八カルシウム・コラーゲン複合体に関する基礎研究から治験までの経過概要（バイオマテリアル-生体材料- 3, pp.88-95）

    川井 忠, 鎌倉慎治, 松井佳子, 高橋 哲, 鈴木 治

    日本バイオマテリアル学会　2019

  9. 医療を支える新素材「リン酸カルシウムによる骨再生」（現代化学, pp.34-36）

    鈴木 治

    東京化学同人　2019

  10. リン酸オクタカルシウム（OCP）の骨再生などへの応用（無機/有機材料の表面処理・改質による生体適合性付与, pp.56-57）

    鈴木 治, 濱井 瞭

    シーエムシー出版　2019

  11. バイオマテリアル？生体材料 30-4

    日本バイオマテリアル学会, 鈴木治

    医学書院　2013/02

  12. 『環境調和型新材料シリーズ 生体材料』

    日本セラミックス協会編, 鈴木治

    日刊工業新聞社　2008/10

  13. Interface Oral Health Science 2007

    M. Watanabe, O. Okuno, K, Sasaki O. Suzuki, N. Takahashi, ed. T, Kawai T, Anada S. Kamakura, Y. Honda, A. Matsui, K. Sasaki, S. Echigo, O. Suzuki

    Springer Japan　2007/12

  14. Interface Oral Health Science 2007

    M. Watanabe, O. Okuno, K, Sasaki O. Suzuki, N. Takahashi, ed. Ichiro, Takahashi Fumie Terao, Taisuke, Masuda Yasuyuki, Sasano Osamu Suzuki, Teruko Takano-Yamamoto

    Springer Japan　2007/11

  15. Interface Oral Health Science 2007

    M. Watanabe, O. Okuno, K, Sasaki O. Suzuki, N. Takahashi, ed. Yuko Suzuki, Shinji Kamakura, Koki, Hatori Kazuo, Sasaki Yoshitomo Honda, Takahisa Anada Keiichi, Sasaki Osamu Suzuki

    Springer Japan　2007/10

  16. Interface Oral Health Science 2007

    M. Watanabe, O. Okuno, K, Sasaki O. Suzuki, N. Takahashi, ed. Fumie Terao Ichiro, Takahashi Hidetoshi Mitani, Naoto Haruyama, Osamu Suzuki, Yasuyuki Sasano, Teruko Takano-Yamamoto

    Springer Japan　2007/09

  17. Interface Oral Health Science (International Congress Series 1284)

    Watanabe M, Takahashi N, Takada H, eds. Noji M, Sasaki K, Kuriyagawa T, Suzuki O, Ogawa K, Kanehira M

    ELSEVIER　2005/06/01

  18. Interface Oral Health Science (International Congress Series 1284)

    Watanabe M, Takahashi N, Takada H eds, Kamakura S, Sasano Y, Suzuki O

    ELSEVIER　2005/05/01

  19. Interface Oral Health Science (International Congress Series 1284)

    Watanabe M, Takahashi N, Takada H, eds. Junko Hatakeyama, Yuji Hatakeyama, Ichiro Takahashi, Osamu Szuki, Yasuyuki Sasano

    ELSEVIER　2005/04/01

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Research Projects 52

  1. Bone substitute materials that promote the materials resorption and new bone formation

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2024/04/01 - 2027/03/31

  2. Development of bone graft substitutes with both osteogenic potential and high strength

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2023/04/01 - 2026/03/31

  3. リン酸八カルシウム・コラーゲン複合体と自家骨とによる広範囲顎骨再建法の確立

    川井 忠, 松井 桂子, 江副 祐史, 鈴木 治, 鎌倉 慎治, 山田 浩之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 岩手医科大学

    2021/04/01 - 2024/03/31

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    ２０２１年度はリン酸八カルシウム・コラーゲン複合体のみをイヌ下顎骨区域切除部位に埋入する手術と、腸骨から採取した自家骨とリン酸八カルシウム・コラーゲン複合体を混合させて埋入する手術を行う計画であった。リン酸八カルシウム・コラーゲン複合体（商品名Bonarc）の販売予定が遅れており、２０２１年度中での購入ができなかった。購入が今後も遅れる場合は、製造元の東洋紡株式会社に大学との共同研究の申請を依頼し、材料の供給を確保することを考えている。イヌを用いた実験内容で岩手医科大学動物実験計画書を申請したが、これまでの経験からイヌでの実験はかなりの労力を費やすことから、現在では手術に伴う時間や人員などの削減のため、同じ中型動物であるウサギの下顎骨を用いた同内容での実験の検討を行っている。今後はウサギでも同内容の実験が可能かを確認しつつBonarcの供給を待つ予定である。当初の予定通りイヌで進める場合は、現在は予定より遅れているため、スムーズに実験を進めるために、これまでイヌでの実験多く進めてきた東北大学の動物実験施設で行うことも検討している。実験の準備としては、下顎骨の区域切除術に使用する医療用器機を購入した。人員も申請した分担者以外にも、当分野の大学院生を数人確保し、研究を行う準備は進めている。また今後の研究へのためにも様々な学会へ参加し、顎骨再建に関わる材料や手法についての演題や講演から研究に参考となる情報を収集した。

  4. プロテオームとミネラロームのイメージングを用いたMMP-2による再生骨成熟の解析

    笹野 泰之, Yang MuChen, 鈴木 治, 矢尾 育子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2021/04/01 - 2024/03/31

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    骨の発生過程で、MMP-2による基質タンパクの分解は石灰化、すなわちミネラル結晶の沈着と成長を進行させ、骨基質を成熟させる。一方、骨の修復過程（再生過程）については知見が乏しい。本研究では、MMP-2欠損マウスを用いて、修復骨（再生骨）のタンパク構成すなわちプロテオームを質量顕微鏡で、ミネラル構成すなわちミネラロームを分析走査電子顕微鏡で、それぞれ可視化し、MMP-2欠損マウスと野生型マウスにおける修復骨の石灰化と成熟を検討することを目的とした。この際、MMP-2欠損マウスの再生骨で分解されずに残存し、野生型マウスで分解される基質タンパクを画像上で選択して同定し、石灰化における基質タンパクの役割を解析する計画とした。 理研バイオリソース研究センター（理研BRC）から導入し、本学の動物実験施設における検疫を経て、研究代表者等の研究グループで繁殖をしているMMP-2欠損マウス（GelA KO）を研究に利用した。当初は生後10週齢のMMP-2欠損マウス及び野生型のマウスに、全身麻酔下で頭頂骨に直径2.4mmの規格化骨欠損を作製し骨修復を検討する計画であったが、本研究の遂行過程で利用するマウスの週齢が修復骨の石灰化に大きく影響する可能性が考えられた。そのため、研究に用いる週齢を再検討することとし、その基礎とするため、若齢マウス（７週齢）と高齢マウス（２５週齢）との間で、骨のミネラル構成（ミネラローム）をマイクロX線CTと分析走査電子顕微鏡で検討する実験を開始した。 生後７週齢および２５週齢のMMP-2欠損マウスおよび野生型のマウスをそれぞれ１１匹、計４４匹を利用した。全身麻酔下に、下肢骨、頭蓋骨、上下顎骨を4％パラホルムアルデヒド、 2％グルタールアルデヒド-2％グルタールアルデヒドまたは95％エチルアルコールで固定した。繁殖期間も含め、全試料の採取に約１年間を要した。

  5. 骨再生能と高弾性を有する骨補填材開発の試み

    鈴木 治, 濱井 瞭, 森 優, 塩飽 由香利

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2021/04/01 - 2024/03/31

  6. Fabrication of a bioactive calcium phosphate implant material

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2021/07/09 - 2023/03/31

  7. New innovation of jaw bone reconstruction using octacalciumphosphate/collagen composite and iPS cells

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2020/04/01 - 2023/03/31

  8. トンネル磁気誘電効果を有するナノ複相構造薄膜の新機能生体センサーの展開

    増本 博, 小林 伸聖, 薮上 信, 曹 洋, 青木 英恵, 池田 賢司, 佐々木 啓一, 鈴木 治

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2020/04/01 - 2023/03/31

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    本研究グループは、磁性ナノ粒子（直径約数nm）が誘電体セラミックス中に均一に分散した薄膜である磁性体－誘電体ナノ複相構造薄膜を作製し、これまでに報告例の無い新しい機能変換材料である『トンネル磁気誘電（Tunneling Magneto-Dielectric:TMR）効果」（磁場を印加すると材料の誘電率が磁場の強さに応じて変化する現象。室温で動作する。本研究グループで命名）』を見いだした。本研究は、このTMD効果の特性向上や原理の究明といった基礎研究を進めるとともに、新しい生体センサーの応用研究という次の段階にステップアップするための基盤的研究を行うことを目的としている。 今年度の成果として、①誘電体マトリックスとして用いるフッ化物（MgF2やBaF2）材料として、新たにSrF2に着目し、共スパッタリング法による高結晶化フッ化ストロンチウム－コバルトナノ複合薄膜の合成を実現し、理論に基づき、マトリックスの結晶化度を上げることでより高いトンネリング磁気誘電効果を得ることに成功した。②誘電体マトリックスとしてフッ化物に代わり各種酸化物を用いたナノ複相構造薄膜（Co-Al2O3、Co-MgO、Co-Ta2O5）を作製し、膜の電気抵抗のCo組成依存性がTMD特性と密接に関連していることを突き止めた。③これまでの磁性体－誘電体ナノ複相構造薄膜では、磁性体はおよそ球体ナノ粒子であったが、扁平ナノ粒子を作製することにより異方性を向上させて、膜の方向によりTMD特性を制御する手法を考案した。

  9. Search for matrix proteins that regulate calcification by analyzing degradome for calcified tissues of MMP 2 - deficient mice

    Sasano Yasuyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2018/04/01 - 2022/03/31

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    This study was designed to search for regulatory matrix proteins in bone and tooth calcification that are degenerated by MMP-2 (Matrix Metalloproteinase-2). The concentration of elements of calcium, phosphorus and carbon was compared between MMP-2 deficient mice (MMP-2 KO) and wild type mice (WT) to understand how deficiency in MMP-2 affects composition of the elements and how the element composition is involved in calcification. Tibiae of MMP-2 KO and WT at 7 weeks were examined with micro-computed tomography, scanning electron microscope with energy dispersive X-ray spectroscopy and histology. The bone mineral density of the cortical bone in tibiae were lower in MMP-2 KO than WT. In contrast, no significant difference was recognized in composition of the elements or in histological observation.

  10. A study on bioactive ceramic biomaterials

    Suzuki Osamu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2018/04/01 - 2021/03/31

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    The present study was designed to investigate about how physicochemical environments affect the activity of calcium phosphate materials. Calcium phosphate materials, including octacalcium phosphate (OCP), cultured with mesenchymal stem cells in the spheroid form, enhanced its osteoblastic differentiation in vitro. The cellular activity was well correlated with mouse calvaria defect bone formation by the materials. OCP/gelatin composite was capable of enhancing angiogenesis followed by bone formation in rat calvaria defect. The dose of OCP in the composite affected bone formation ability in rat model under osteoporotic serum environment. The chemical environment surrounding the bone substitute materials may affect the activity of calcium phosphate materials in particular OCP.

  11. Reconstruction of a bone development model by a 3-D cell culture system

    Anada Takahisa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    2017/06/30 - 2020/03/31

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    The formation of bone occurs by intramembranous or endochondral ossification. Endochondral ossification takes place during the transition of soft to hard callus. In this process, various molecules and their concentration gradient around cells play an important role in the cell differentiation of stem cells. We proposed a novel 3D cell culture system as a model of the endochondral ossification. The purpose of this study was reconstruction of a 3D tissue model for the chondrogenesis and invasion by capillaries to cartilaginous callus.

  12. Model construction for molecular regulatory mechanisms in condensation of mesenchymal stem cells during organogenesis

    Takahashi Ichiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Kyushu University

    2017/04/01 - 2020/03/31

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    Cell condensation is a critical role for the organ morphology. However, the mechanisms are still unclear. In this study, we explored specific transcriptional start sites (TSS) of tooth organ by CAGE (Cap Analysis of Gene Expression) analysis. We identified a tooth specific TSS, which has been detected on chromosome 15qD1 region. This TSS codes microRNA-875 (mir875). MiR875-5p was specifically expressed in dental mesenchymal cells at bell stage of tooth germ.We identified that the cell migration toward to dental epithelial cells was significantly induced by miR875-5p via PDGF signaling pathway.

  13. Fabrication of 3-D cellular constructs using gel transfer methods

    Anada Takahisa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    2017/04/01 - 2020/03/31

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    Three dimensional cellular constructs which are formed in vitro are called as 3D organoids or miniature organs. Construction of 3D organoids is one of the most important issue in tissue engineering. In this study, we have developed a novel 3D organoid culture system based on an oxygen permeable cell culture device. Three dimensional vascular-like structures were formed by use of different types of spheroids embedded in hydrogels. We found that the reconstruction techniques for the active and vascularized cellular constructs improve cell-cell interactions between osteoblasts and endothelial cells and are effective on the differentiation of cells.

  14. Development of biodegradable osteosynthesis device activating osteoblast cell

    Miyatake Naohisa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2016/04/01 - 2020/03/31

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    The present study was designed to investigate whether octacalcium phosphate (OCP)/ biodegradable polymer composite can be used as an artificial bone that has osteoconductivity. Bone formation was enhanced by the OCP/ poly lactic-co-glycolic acid (PLGA) composites compared to PLGA alone, through enhanced osteoclastic resorption of OCP. Our earlier study showed that OCP/gelatin (Gel) composites rapidly biodegraded and promoted the formation of new bone compared to commercially available β-TCP. The present study suggests that octacalcium phosphate/ biodegradable polymer composite could be used as an osteoconductive and biodegradable material through the comparative study with HA / poly-L-lactic acid (PLLA) composite or βTCP /PLGA composite which are commercially available as the biodegradable material.

  15. A study of bioactive materials used for bone filling

    Suzuki Osamu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2017/06/30 - 2019/03/31

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    The present study was designed to investigate about how chemical environment around calcium phosphate crystals affects proteins accumulation onto the crystals. Co-precipitation of bovine serum albumin as a model protein for active molecules, such as proteins in immunomodulation, was controlled by the solution calcium and phosphate ions environment under physiological conditions. The results suggest that the design concept to accumulate the proteins on the materials. The concept of the materials accumulating active molecules obtained in the present study could be used for the tissue regeneration.

  16. Study on practical application of innovative prevention and treatment method for old people's root caries by formation of fluorinated apatite film

    Akatsuka Ryo

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2016/04/01 - 2019/03/31

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    This study aimed to create a thick hydroxyapatite (HA) film on the surface of a human tooth via a powder jet deposition (PJD) device for dental handpieces, and to examine the microstructural and mechanical properties of the FHA film. The FHA particles in the deposited film were densely packed, and the three-dimensional microstructure and the rough surface of the FHA film were unchanged after thermal cycling. There were also no significant differences in the hardness and the bonding strength between the FHA film before and after thermal cycling.

  17. Identification of ECM proteins that inhibit calcification in bone and dentin using matrix-assisted laser desorption/ionization imaging mass spectrometry

    SASANO YASUYUKI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2019/03/31

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    The study was designed to investigate matrix molecules characteristic for bone and dentin using matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS). We examined methods to apply IMS for the calcified tissues and adopted 4% paraformaldehyde fixation - freeze with or without decalcification in 10% EDTA since information about the tissue preparation was not available. Developing rat teeth at 3 days were processed for IMS analysis. The data was acquired in the positive ion mode with 75μm and 10μm pitches and a mass range setting of 0 to 1000. Characteristic localization of signals in the tooth germs was shown at m/z 706 and 732. The signals were localized in the area of dental papillae and enamel epithelia in the tooth germ. The data base search indicated that the corresponding molecules are phosphatidylcholines. Sudan Black B staining showed comparable localization of lipids in the tooth germ.

  18. A study of crystal growth and its relation to bioactivity of calcium phosphate materials

    Suzuki Osamu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2017/03/31

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    The present study was designed to investigate as to whether calcium phosphate precipitation and its crystal phases affect its bioactivity of materials used for bone substitute materials. The materials, synthesized in batch or continuous preparations, were made through wet and dry mixing procedures and subjected for analyses by physical techniques, such as Raman spectroscopy, to indentify the crystal phases included. It was suggested that the activity of osteoblastic cells could be influenced by distinct crystals of octacalcium phosphates obtained in the continuous preparation.

  19. Construction of mesenchymal stem cells-calcium phosphate hybrid spheroids using an oxygen-permeable cell culture device

    Anada Takahisa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    Three-dimensional (3D) cellular constructs have been paid attention in the field of tissue engineering. Increase in size of cellular aggregates causes a depletion of oxygen in the core of aggregates. We have developed an oxygen-permeable cell culture device to prevent hypoxia in the core of the cellular aggregates. The aim of this study was construction of 3D cellular structure consisted of mesenchymal stem cells (MSCs) and calcium phosphate materials based on the oxygen-permeable device we developed. We found that the device was able to improve osteoblastic differentiation of MSCs and generate hybrid spheroids consisted of cells and calcium phosphates. These results suggest that the device is useful for engineering 3D cellular constructs for tissue engineering.

  20. The development of a novel preventive method of periodontal disease using S-PRG Filler eluate

    Iwamatsu-Kobayashi Yoko, SUZUKI OSAMU

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    S-PRG filler, a component of composite resin, is capable of releasing 6 metal ions that possess antibacterial activity against caries and periodontal pathogens. Although S-PRG has been suggested to be involved in oral disease prevention, no reports have been published regarding its preventive effect on periodontal disease in vivo. The present study investigated whether the eluate from S-PRG (S-PRG eluate) has a preventive effect on a mouse model of ligature-induced periodontal disease. C57BL/6 mice were divided into three groups; no ligature, ligature and ligature with S-PRG eluate group. S-PRG eluate clearly inhibited alveolar bone loss of ligature-induced periodontal disease and reduced the infiltration of inflammatory cells. TOF-SIMS analysis revealed that more boron ions were present in the S-PRG group. Our results suggest that the S-PRG eluate has a preventive effect against tissue destruction in periodontal disease through its anti-inflammatory effects in vivo.

  21. Development of new evaluation system of mucosal inflammation based upon vascular imaging and preventive method of oral mucositis with oral mucosa mapping system

    KOSEKI Takeyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    To prevent the oral mucosal problem and to enhance the promotion of oral-related QOL, a preventive method of oral mucositis and mucosal injure was built for the elderly suffering xerostomia and for the patients who were undergoing perioperative medical treatments. The updated technology of visual examination of mucosa, so-called Narrow Band Imaging, was employed in this system to visualize the abnormality of oral mucosa. The oral mucosa mapping system which covered whole oral cavity was also developed. By using these two systems, new diagnostic method of oral mucosa was proposed to prevent oral mucositis. Finally, the comprehensive guideline of diagnosis and treatment of oral mucositis were discussed.

  22. A study of materials property and osteoconductive capacity of bone substitutes

    Suzuki Osamu, ARAI FUMIHITO

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    The present study was designed to investigate as to whether calcium phosphate in matrix polymer materials affects oriented bone formation. Octacalcium phosphate without the matrix stimulated macrophage-like cells migration. The presence of gelatin controls the crystallite size and the hydrolysis rate. Octacalcium phosphate in gelatin matrix affects the production of oriented bone formation in rat calvaria defect. The composite stimulated the formation of cortical bone having strength compatible to natural bone in rabbit tibia defect (push-in-test). The presence of octacalcium phosphate in the matrix material could be associated with the quality of newly formed bone.

  23. Construction of cell-based composite tissue by using an oxygen permeable culture device

    Anada Takahisa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    Engineering three-dimensional (3D) cellular constructs outside of the body is one of the most important issues for tissue engineering. Increase in size of cellular aggregates causes a depletion of oxygen in the core of aggregates. We have developed an oxygen-permeable cell culture device to prevent hypoxia in the core of the cellular aggregates. The aim of this study was construction of 3D cellular structure consisted of mesenchymal stem cells (MSCs) and vascular endothelial cells based on the oxygen-permeable device we developed. We found that the device was able to improve osteoblastic differentiation of MSCs and generate cellular aggregates consisted of two types of cells. These results suggest that the device is useful for engineering 3D cellular constructs for tissue engineering.

  24. Clarification of bone mineralization process and hard tissue construction

    Suzuki Osamu, ANADA TAKAHISA, SHIWAKU YUKARI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: Tohoku University

    2011/04/01 - 2016/03/31

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    The present study was designed to investigate whether the arrangement of calcium phosphate mineral crystals in the matrix scaffold affects the cellular activity around the crystals, including the differentiation from the precursor cells, in the mineralized tissue formation. It was apparent that the differentiation of mouse bone marrow derived stromal cells is controlled by the chemical environment associated with the crystals, that the differentiation of murine bone marrow derived mesenchymal stem cells is controlled by the crystal phase and the amount of strain of the matrix around the cells, and that the tissue formation, including the formation of the mineralized tissue and the bone-related non-mineralized tissue, is controlled by the dispersion condition of the crystals in the matrix scaffold material. The presence and the characteristics of the mineral crystals could be a factor to control the mineralized tissue formation.

  25. Research Management

    Arai Tatsuo, ARAI Fumihito, Yamato Masayuki, KANEKO Makoto, NAKAUCHI Hiromitsu, FUKUDA Toshio, SEKI Minoru, TAKEUCHI Syouji, SUZUKI Osamu, MAE Yasushi, KANEKO Makoto, NAKAUCHI Hiromitsu, FUKUDA Toshio, SEKI Minoru, MAE Yasushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: Osaka University

    2011/04/01 - 2016/03/31

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    We coordinated research groups closely in the area and proceeded fusional research, and presented our research activities in wide area of the domestic and international societies. We exchanged the newest research results and shared knowledge regularly among the research groups in the area through the management meetings and the whole research meetings to make our studies more active. In four main research groups, each leader coordinated research groups and proceeded research by exchanging new results and sharing knowledge through regular group meetings.

  26. A study of hydrated layer of calcium phosphate surface and its osteoconductivity

    SUZUKI Osamu, KAMIIE Junichi, ANADA Takahisa, KAWAI Tadashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04/01 - 2015/03/31

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    The present study was designed to investigate about whether surface hydrated structure of calcium phosphate materials is associated with their biocompatibility and osteoconductivity. Fluoride-containing hydroxyapatite was synthesized according to our previous report (Shiwaku et al. Acta Biomater 2012) and subjected to analyze non-apatitic and apatitic HPO4 and OH ions in the structure by FTIR cure fitting. The hydrolysis progress was shown to relate to the change of HPO4 content. Because it has been suggested that the conversion process from HA precursor to HA may be involved in increasing the osteoconductivity of the calcium phosphate materials, the degree of crystallization from the precursor phases to HA was investigated by immersing the HA precursors in simulated body fluid. It was apparent that the hydrolysis of OCP is enhanced in the co-presence of ACP and the process can be detected by monitoring the surface OH ions in the hydrated structure through FTIR cure fitting analysis.

  27. REGENERATION OF A RABBIT TIBIA BONE USING A NEW BONE SUBSITUTE MATERIALACTIVATING OSTEOBLAST CELL

    NAOHISA miyatake, SUZUKI Osamu, IMAIZUMI Hideki, ITOI Eiji, HOSAKA Masami

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    This study was designed to investigate whether an octacalcium phosphate/gelatin (OCP/Gel) composite can repair a defect created in rabbit tibia for 2, 4, 8 weeks. Histological histomorphometrical and microCT studies showed that implantation of OCP/Gel composite enhanced bone regeneration in the defect compared to other bone substitute materials such as hydroxyapatite and b-TCP. Furthermore, the results demonstrated that OCP/Gel composite was a highly bioderadable material in rabbit tibia. The present study suggests that the OCP/Gel composite is a bone substitute material in which the stimulatory capacity of OCP crystals in new bone formation can be expressed even in the defect of long bone.

  28. Development and proposal of evaluation method of oral mucosal damages based on the degree of injuries and atrophy of oral mucosa and saliva properties

    KOSEKI Takeyoshi, TANDA Naoko, HOSOKAWA Ryoichi, SUZUKI Osamu, ITOU Emi, TAURA Katuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2011/04/01 - 2014/03/31

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    Novel diagnostic method and preventive treatment system of oral mucosa had been developed based upon the evaluation of the degree of injury and atrophy, to prevent oral problems of the elderly and side effects of cancer treatment. For this purpose, we established the haptic diagnostic devices of oral mucosa with the technology of ultrasound sensing. Using this device and conventional evaluation systems of oral environment, we analyzed the symptoms of injured oral mucosa, and objective estimation of damage on oral mucosa had advanced to utilize for the clinical applications. Based upon these results, total management plans to maintain the health of oral mucosa were discussed.

  29. Creation of next generations of periodontal tissue engineering by applying honeycomb microarrays

    SHIMAUCHI Hidetoshi, ISHIHATA Hiroshi, NEMOTO Eiji, SUZUKI Osamu, KANAYA Sousuke, SHIMOMURA Masatsugu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2011/04/01 - 2014/03/31

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    This research project was aimed to develop the new strategy for periodontal regeneration by combining both technologies of honeycomb microfilm (HF) and conditioning of the extracellular microenvironment for periodontal stem cells. As the result of studies, we indicated that: 1) a biodegradable polymer HF is suitable for the carrier of periodontal ligament cell sheet, 2) autocrine secretion of prostaglandin E2 is essential for the differentiation of periodontal cells stimulated with Ca2+ ion and mechanical stress, and 3) nano-hydroxyapatite could upregulated BMP-2 expression independent of ion release. Furthermore, OCP/ gelatin composite inlay increased angiogenesis as well as bone regeneration in the rat calvaria defect model. Our research results well established the foundations of new concept scaffold technology that can stimulate both cell proliferation and differentiation.

  30. A study of bioactive materials used for filling bone defects

    SUZUKI Osamu, ITOI Eiji, KAMAKURA Shinji, ANADA Takahisa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2011/04/01 - 2014/03/31

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    The present study was designed to investigate the effect of calcium phosphate crystal characteristics on the bioactivity of the bone substitute materials. The spongy composite consists of synthetic octacalcium phosphate (OCP) and natural polymers, such as gelatin, was prepared. OCP crystals, ground mechanically from micrometer to nano-submicro meter scale in size, were dispersed in gelatin matrix and molded. The composite was soaked in simulated body fluid for one week or implanted in rat calvaria critical-sized defect for 8 weeks and then analyzed. X-ray diffraction of the soaked materials showed that hydroxyapatite became detected with the time. Histological analysis of the decalcified specimens of the calvaria indicated that new bone was formed together with the partial biodegradation of the materials. The results suggest that the crystal characteristics may be a factor to affect the bioactivity of the composite.

  31. Creation of functional interface on dentin by powder jet deposition

    NOJI Miyoko, SASAKI Keiichi, AKATSUKA Ryo, SUZUKI Osamu, KURIYAGAWA Tsunemoto

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2011 - 2013

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    A powder jet deposition (PJD) process can be used to create a thick hydroxyapatite (HA) film on the surface of a human tooth. In the experiment using dentin, class V cavities were prepared on the buccal surfaces of the teeth of beagle dogs and filled with (1) resin composite on the HA film, (2) zinc oxide-eugenol cement as a negative control group, or (3) zinc phosphate cement as a positive control group. In the experiment using enamel, the enamel was polished and coated with (1) the HA films, (2) resin sealants as one negative control group, and (3) glass ionomer cement as the other negative control group. No severe inflammatory responses were observed in all specimens applied by PJD, showing even milder response than that of specimens used as negative control. The bacteria were not identified in the pulp space or dentinal tubules in any postoperative periods.

  32. Promotion of bone formation using chondroitin sulfate E and application to bone regenerating biomaterials

    MIYAZAKI Tatsuya, SUZUKI Osamu, ANADA Takahisa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2011 - 2013

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    Chondroitin sulfate E enhances osteoblast differentiation and inhibits osteoclast differentiation. As a mechanism of action, it was demonstrated that CS-E bound to adhesion molecules such as both osteoactivin (OA) and its receptor, integrin. And also CS-E inhibited the osteoclast differentiation by blocking the interaction of OA to integrin and heparan sulfate. On the other hands, osteoblast produced biglycan core protein and E-unit of CS. The amount of these molecules transiently increased before osteoblast differentiation (mineralization). These results indicated that not only the exogenously-added CS-E but also endogenous CS contributed the differentiation of the cells. Based on the results in the present study, we will further investigate the potentiality of CS-E as an application to bone regenerating biomaterials.

  33. A study of a synthetic construct for bone development

    SUZUKI Osamu, SHIMAUCHI Hidetoshi, KAMAKURA Shinji, ANADA Takahisa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

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    Synthetic calcium phosphates, having a higher adsorption affinity with non-collagenous proteins, such as albumin, were prepared as a source material for an implantable construct. Fluoride-containing calcium phosphates, showing a relatively higher solubility and a higher protein adsorption affinity, were developed. Natural polymer, such as alginate, was used to study for the preparation of the implantable gel form.

  34. Development of drug carrier for bone regeneration

    ANADA Takahisa, SUZUKI Osamu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

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    Novel calcium phosphate-binding lipids were synthesized. The polymer modified lipids were incorporated efficiently into liposomes. We found that the calcium phosphate binding and drug encapsulation efficiencies of the polymer modified liposomes were higher than those of the unmodified liposomes.

  35. A study of injectable osteoconductive bone substitute materials

    IMAIZUMI Hideki, SUZUKI Osamu, ITOI Eiji, KISHIMOTO Koushi, ANADA Takahisa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2009 - 2011

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    Octacalcium phosphate(OCP)crystals, having particular stoichiometeric composition, have been shown to display a stimulatory capacity on osteoblastic differentiation and bone regeneration. Synthesized OCP crystals show higher biocompatibility and are expected to be an artificial bioabsorbable material. On the other hand, its brittleness based on the ceramics characteristics makes it difficult to mold the objective shape. In the present study, we created a new OCP materials combined with hyaluronan(Hya), which could be used as the injectable form, and examined their bone regenerative properties on mouse calvaria.

  36. 破骨細胞培養系における非侵襲的細胞回収用装置の開発と応用

    穴田 貴久, 鈴木 治, 溝口 利英

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的萌芽研究

    Institution: 東北大学

    2009 - 2010

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    細胞凝集塊(スフェロイド)培養は、三次元的に細胞が増殖するため、通常行われる単層培養に比べ、より生体内環境に近い状態での培養が可能となる。肝細胞はスフェロイド培養により細胞活性の長期維持が可能になることが知られている。また、癌細胞をスフェロイド化することで単層培養よりも生体内環境に近い薬物代謝解析が可能になると言われている。細胞のスフェロイド培養は応用性が高く、今後、創薬や再生医療においてよりニーズが高くなると考えられるが、これまでスフェロイド培養には煩雑な操作や高度な技術が必要であった。そこで、我々は、再生医療や薬物代謝解析に応用可能なスフェロイド培養装置の開発を行っている。昨年度までに作製したスフェロイド培養装置は、ポリジメチルシロキサン(PDMS)薄膜、アクリル板、ガラス板を材料としてたもので、細胞をこの装置へ播種するだけで大きさが均一なスフェロイドを大量に形成させることに成功した。この成果は、海外雑誌に受理されている(Sensors and Actuators B 2010,147,376)。本年度はさらに簡便に量産化可能な培養装置を開発することに成功した。これはPDMSだけ材料としており、より安価で簡便なスフェロイド培養が可能となった。この成果は第10回再生医療学会総会で報告している。この培養器にマクロファージRAW264.7細胞を播種し、破骨細胞誘導因子であるRANKLを添加すると培養器内に破骨細胞が形成することを確かめている。培養器に播種する細胞数を変化させることで形成する破骨細胞の大きさが変わることを見出した。また、本培養器を用いるとこれまでには困難であった破骨細胞の非侵襲的回収が可能であることがわかった。

  37. 骨芽細胞の増殖分化を促進するリン酸オクタカルシウムコア成長因子徐放デバイス開発

    鈴木 治, 島内 英俊, 鎌倉 慎治, 穴田 貴久

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的萌芽研究

    Institution: 東北大学

    2008 - 2010

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    最終年度である平成22年度は,平成21年度までに開発したフッ素(F)含有OCP(F-OCP)のタンパク質吸着能を調べた.F-OCPは,合成済みのOCPをFイオン存在下で中性からアリカリ領域の範囲で生理的温度下,加水分解させる系(Hydrolysis F:HF-CaP)および,Fイオンの共存下でOCPを共沈殿させる系(Co-precipitation F:CF-CaP)で調整した.合成試料のX線回折および化学分析によると,低濃度のFを含むF-OCPは,HF-CaP,CF-CaPともにOCPの結晶構造を基本的に維持した.試料の単位面積当たりの成長因子モデルタンパク質としてのチトクロムc吸着量は,HF-CaPがより良い吸着媒として働いた.次に,亜鉛(Zn)を含むOCPを同様の合成手法にて調整した.得られたZn-CaPでは,X線回折およびFTIRの分析から,非晶質様の低結晶性アパタイトが形成されたが,OCPが有する溶解性に近い溶解性を示しており,生体内でOCPが吸収されるに伴ってZnイオンが徐放される可能性が示された(Honda Y,Anada T,Suzuki O et al.Effect of Zn^<2+> on the physicochemical characteristics of octacalcium phosphate and its hydrolysis into apatitic phases.Crystal Growth and Design 2011 in press.Publication Date (Web):March 25,2011.DOI:10,1021/cg1009835).以上,本研究により骨芽細胞を活性化する無機の因子を含有する新規のリン酸オクタカルシウムコア成長因子徐放デバイスの開発を達成することができた.

  38. A role of MMP family molecules in remodeling ECM and advancing calcification

    SASANO Yasuyuki, NAKAMURA Megumi, AKITA Hirotoshi, SUZUKI Osamu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2008 - 2010

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    An organ culture system to model the physiological calcification process was designed using rat embryonic calvaria as a device for analyzing its mechanism. Standardized calvarial explants were dissected from rat embryos aged day 18 and cultured for 1, 3, and 5 days. The Ca content was examined with atomic absorption spectrophotometry and distribution of Ca and the concentration of Ca, P and C were examined with von Kossa stain and/or SEM-EDX. The result indicates that the organ culture system using E18 calvaria is useful for modeling of the physiological calcification process in vitro.

  39. Development of accelerated remineralization method based upon the non-invasive assessment of mineral density of tooth

    KOSEKI Takeyoshi, TAKAHASHI Nobuhiro, SUZUKI Osamu, TAURA Katsuhiko, IKAWA Kyoko, TANDA Naoko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2008 - 2010

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    Ultrasonic measurement system for mineral density of tooth surfaces had been developed. This system actualized significantly sensitive, non-destructive, and quick measurement. Using this system, we analyzed the relationship between mineral density of tooth and oral environment, especially function of saliva. The result revealed that acid resistivity was correlated with ultrasonic density of tooth mineral. This indicated the possibility of developing new preventive program against tooth decay according to the ultrasonic density of tooth mineral, buffering capacity of saliva, and living practice of daily life.

  40. Creation of the new periodontal tissue engineering by dual delivery

    HIDETOSHI Shimauchi, EIJI Nemoto, KANAYA Sousuke, SUZUKI Osamu, SASANO Yasuyuki, KAMAKURA Shinji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2008 - 2010

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    The purpose of this study is to develop the new concept of periodontal tissue regeneration which has a high-efficiency and is propagable to general dentistry. It was revealed that an application of newly-developed scaffold induced the proliferation and control of extracellular inorganic ion concentration induced the expression of a variety of cell growth factors, resulting in the enhanced proliferation and differentiation of periodontal component cells, as well as other hard tissue forming cells. Our study strongly suggested a possibility for the development of periodontal tissue engineering by applying controlled release of inorganic ions and surface topography of scaffold combined with cytokines, without using cell therapy.

  41. Creation of highly functionalized bone regenerative material based on octacalcium phosphate (OCP) collagen composites

    KAMAKURA Shinji, ECHIGO Seishi, SUZUKI Osamu, MATSUI Keiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2008 - 2010

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    Bone regenerative properties of various octacalcium phosphate (OCP)?collagen composite (OCP/Col) were investigated. (1) OCP/Col promoted the proliferation and attachment of cells. (2) Bone regeneration by implantation of OCP/Col in small animals was improved by dose-dependent manner of OCP content. The quality of the regenerated bone by implantation of OCP/Col was progressively increased and was compatible to that of normal bone of mechanical properties. (3) In various implantation designs of large animals, sufficient bone regeneration was confirmed by implantation of OCP/Col. These sequential fruitions led to the first clinical trial of OCP/Col implantation in the world.

  42. パウダージェット法と硬組織自己再構成法による瞬時小窩裂溝填塞術の開発

    小関 健由, 佐々木 啓一, 鈴木 治, 田浦 勝彦, 丹田 奈緒子, 細川 亮一, 井川 恭子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的萌芽研究

    Institution: 東北大学

    2008 - 2009

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    パウダージェットデポジション(PJD)法を応用して歯質上に歯の主成分であるハイドロキシアパタイト(HAp)膜の生成し,歯質-生体材料間に全く新しいインターフェイスの創成を実施した。エナメル質の成膜に関しては、PJD装置、HAp微粒子,噴射条件等を検討してきた結果、小窩裂溝内への成膜が確認された。特にPJD装置では、実験用装置ではなく、ハンドピース型装置での成膜が可能になっており、実際の治療に準じたハンドピース先端の噴射ノズルの速度、角度等の条件を自由に設定することにより、より良い成膜条件の検討を可能とした。様々な粒子半径、焼成温度のHAp微粒子を使用し、成膜に適した条件(粒子半径3-4μm,焼成温度1200℃)を明らかにした。さらに、HAp膜の象牙細管透過性へ与える影響を中心に評価を行ったところ、HAp粒子が象牙細管に入り込み、象牙細管開口部を閉鎖し、象牙細管透過性が低下することが判明した。また、条件を変化させることで、PJD装置をそのまま利用して、歯質を切削するアブレイシブジェット加工も行えることが判明した。これらの結果から、今回申請した方法で、軟化象牙質と脱灰エナメル質の選択的除去し清掃された硬組織の実質欠損部分に、PJD法にてハイドロキシアパタイトの歯質と一体化したハイドロキシアパタイト膜を形成させて象牙細管の封鎖と歯髄の保護を行う裏層を施し、その上で充填術を施す全く新しい治療法の可能性が示唆された。

  43. Antineoplastic effect of octacalcium phosphate with osteoconductive characteristics

    HATORI Masahito, SUZUKI Osamu, ITOI Eiji, HOSAKA Masami

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2007 - 2009

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    The purpose of this study is to evaluate antineoplastic effect of octacalcium phosphate(OCP) with osteoconductive characteristics. EES1, newly-established cell line of Ewing's sarcoma were cultured and evaluated for its growth potential on OCP-coated and hydrolyzate(HL)-coated plates and control.As compared with the control, EES1 growth was suppressed on both OCP-coated and HL-coated plates. C2C12, mouse myoblast cell line was also suppressed on OCP-coated and HL-coated plates. The present study suggests potential application of OCP with various kinds of crystallinity as a bone substitute for the treatment of bone and soft tissue tumors.

  44. Analysis of proteins accumulated onto octacalcium phosphate and the related calcium phosphate crystals

    SUZUKI Osamu, KAMAKURA Shinji, KAMIIE Junichi, ANMIIE Takahisa

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2007 - 2009

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    Synthetic octacalcium phosphate (OCP) enhances bone formation and accumulates circulating serum proteins on its surface if implanted in bone defect. The rat serum proteins accumulated on OCP in vitro preparation were analyzed by proteomic approaches using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Over 100 proteins were detected from OCP surface as cellular and extracellular components. The extracellular components were categorized into molecular function, biological process and intracellular elements based on Protein ANalysis THrough Evolutionary Relationships classification system.

  45. Applied study to bone augmentation using platelet rich plasma and octacalcium phosphate

    KIMIZUKA Satoshi, KAMAKURA Shinji, SUZUKI Osamu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2006 - 2009

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    We used an animal model to evaluate for bone regeneration by maxillary sinus floor augmentation with OCP/Collagen and palatelt-rich plasma(PRP) gel. With an extraction specimen after bone transplantation eight weeks, we compared it with a group of control for roentgenology and developed a non-transmitted image significantly. The extraction specimen accepted remarkable new bone formation on examination of 3 dimensions by a micro X-ray computed tomography scan. From the result, OCP/Collagen and bone regeneration method with PRP gel do not need autograft of bone, and it is the methods that are the utility which bone regeneration can do without adding the second operation field.

  46. A study on mechanical stress and local environment for calcified tissue regeneration

    OSAMU Suzuki, SASAKI Keiichi, KAMAKURA Shinji, TAKAHASHI Ichiro, NAKAMURA Masanori, KAMIJO Ryutaro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research on Priority Areas

    Institution: Tohoku University

    2005 - 2009

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    The present study demonstrated that local environments, such as mechanical stress, control quantitatively osteoclastic cell-mediated biodegradability of apatite precursor (synthetic octacalcium phosphate, OCP), resulting in the loss of bone regenerative capability of this material through strain-dependent stimulation of the osteoblasts around the implanted OCP with collagen matrix. The mechanical stress could be a key factor controlling the morphology of the calcified tissue regeneration during bone and cartilage formation.

  47. 力学適応性傾斜機能を有する生体-インプラントインターフェイスの創製

    佐々木 啓一, 増本 博, 鈴木 治

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 萌芽研究

    Institution: 東北大学

    2007 - 2008

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    ECRプラズマ酸化法により生成される多孔性チタニア膜が生成条件のちがいにより、ナノ・ミクロ構造および結晶組成が変化する。そこで本研究の目的は、ECRプラズマ酸化法による多孔性チタニア膜の生成条件、膜厚、ナノ・ミクロ構造を明らかにし、さらにこれらと生体親和性、細胞親和性などとの関連を明らかにすることである。 本年度は前年度に続き金属材料研究所で開発したECRプラズマ装置を用い鏡面研磨されたチタン基板上にチタニア膜を作製し、その特性の評価を行った。前年度の実験より、ECRプラズマ酸化時のチャンバー内全圧を高真空にすることにより、高温になるほどダメージを受けやすいチタニア膜の低温・短時間での生成を可能にした。さらに、本年度の引っ張り試験の結果、チャンバー内全圧を低真空から高真空にするにつれチタニア膜のチタンとの密着度が増加し、チャンバー内全圧0.015Paで613kg/cm^2という高い密着強度を示し、それ以上の高真空状態では密着強度は減少した。これは前年度の石灰化試験における石灰化能の促進条件と一致し、チャンバー内全圧0.015Paにおいてチタニア膜密着強度と石灰化能の促進が示された。また、この条件下で生成されたチタニア膜はぬれ性試験の結果、生成後まもなくにおいて超親水性を有することが観察された。これらの結果より、ECRプラズマ酸化により生成されたチタニア膜の生体内での骨親和性を期待できる結果が得られた。

  48. Three-dimensional bone formation by octacalcium phosphate combined with collagen

    KAWATA Tetsuo, SASAKI Keiichi, SUZUKI Osamu, KAMAKURA Shinji, KOYAMA Shigeto, KOHKI Hatori, OGAWA Toru, KUBO Kei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2006 - 2008

  49. 生体成分のファウリングを阻止して清浄性を維持する人工材料表面の開発

    鈴木 治, 高橋 信博, 佐々木 啓一, 島内 英俊

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 萌芽研究

    Institution: 東北大学

    2006 - 2007

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    平成19年度は、光重合型レジン表面硬化材にリン酸エステルを含浸したものをアクリル表面に塗布し、表面電位(ゼータ電位)、接触角、モデルタンパク質の吸着特性を測定した。タンパク質として、1)牛血清アルブミン(酸性)、2)ミオグロビン(中性)、3)チトクロームc(塩基性)を用いた。表面改質材として、無機フィラー含有硬化材(市販のナノコートラボ)にリン酸エステルモノマーを1、3、5%含有したものをアクリル表面(角材)で光硬化させ表面をコートしてこれを吸着媒とした。タンパク質溶液(25mM Tris-HC1,pH7.2)を角材の表面に滴下し、室温・湿潤雰囲気下で一定時間保持した後、経時的にサンプリングしブラッド法によるタンパクアッセイを行い、上澄みのタンパク濃度からアクリル表面に吸着したタンパク量を間接的に定量した。その結果、リン酸エステルの含有量またタンパク質の種類により吸着特性に違いが見られ、特に酸性および塩基性タンパク質で吸着に抑制効果があることが示唆された。ゼータ電位はアクリルでは弱く正にチャージしていたが、リン酸エステルを含有すると負のチャージとなった。また、接触角はリン酸エステルが増えるほど小さくなる傾向があった。このことからリン酸エステル含有による表面改質が口腔内タンパク質群の電荷の違いによる表面相互作用を調節する可能性およびその後の口腔内細菌の付着の調節につながる可能性が示唆された。 平成18年度に作製検討していたリン酸化ポリビニルアルコールはリン酸化率17.7%の合成に成功した。しかし、アクリル樹脂との相溶性が低く複合体は作製できたが、その均一分散性にやや難点があり課題を残した。解決策としてリン酸エステルの高分子化を図りそれをアクリルの重合時に加えることで相溶性を高められる可能性があり、本研究から新しい材料作製の着想につながった。

  50. サイトカイン徐放性を有する歯周組織再生deviceの開発

    島内 英俊, 鈴木 治, 笹野 泰之, 鎌倉 慎司, 根本 英二

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 萌芽研究

    Institution: 東北大学

    2005 - 2006

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    本研究の目的は、歯周組織を構成する細胞の増殖・分化のステージに応じた複数のサイトカインの選択的投与が必要という考えから、担体そのものにサイトカインを結合させて徐放させるというシステムを開発することである。平成17年度においては、OCP加水分解物(fiuoridated Ca-deficient HA ; F-HA)をラット頭蓋冠欠損モデルに埋入したところ有意に高い骨再生が得られ、F-HAが代替骨として非常に有用であることをまず示した。本年度はまず骨芽細胞系のST-2のOCPあるいはHAコートディッシュ上での増殖と分化を調べたところ、ST-2のOCP上での細胞増殖は初期において阻害されるものの石灰化能は培養終期に急速に増加することが明らかにあり、OCPは骨芽細胞によってHAに転換されることで硬組織形成を促進することが示された。一方、硬組織形成を促進するサイトカインであるBMP-2とOCPを同時にラット頭蓋冠欠損モデルに移植したところ、顕著に骨再生を促進することが明らかとなった。この作用をin vitroで解析したところ、BMP-2活性は吸着温度条件に依存することが示された。さらにBMP-2吸着はOCPの表面結晶構造により異なることも明らかとなった。マラッセ上皮細胞からの産生が報告されているIGF-IおよびIGF-IIを用いて、マウスセメント芽細胞に分化に与える影響を検討した。その結果、IGF-IIの刺激によって、osteogenic geneのオステオカルシン、骨シアロプロテイン遺伝子発現が有意に亢進することが明らかとなった。また、分化マーカ・一であるアルカリンフォスファターゼの発現も弱いながらも誘導されることが分かった。IGF-Iの刺激においては、著明な変化は見られなかった。一方、セメント芽細胞の前駆細胞と考えられているマウス歯小嚢細胞ではこれらの遺伝子発現はむしろ抑制されることが明らかとなった。すなわち、IGF-IIの作用はtargetとなる細胞の分化段階に大きく依存していることが示唆された。

  51. パウダージェットデポジション法による歯質・生体材料間の新インターフェイス創成

    佐々木 啓一, 厨川 常元, 鈴木 治, 川田 哲男

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 萌芽研究

    Institution: 東北大学

    2005 - 2006

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    本研究は,歯の主成分であるハイドロキシアパタイト(以下HA)微粒子を歯質上に高速で吹きつけ,接着材を介することなく直接HA厚膜を形成することで全く新しい歯質-生体材料間インターフェイスを創成し,新たな歯科治療を提案することである.本研究は大きく3グループ((1)PJD装置開発(2)HA開発(3)臨床試験,評価)に分けられる. (1)に関しては,歯科用PJD装置の試作を行った.HA粉体はガラス容器に窒素充填した状態で提供する方式とし,そこからサーボモータ駆動の微量供給ピストンを用いて押し出す方式を新たに考案した.また装置全重量は,歯科医師が常用可能な150から180gの範囲になるように小型軽量化を図った. (2)に関しては,HA粉合成条件を種々検討し,結晶性の高い粉体が合成でき,かつその粒度分布が2-3μmで一定になるような条件を見いだした.(2)によって合成したHA粉体を,(1)で試作した装置により噴射実験を行い,成膜状態を確認した.またその機械的性質,人工唾液中での形態変化等を観察した. (3)に関しては,(1),(2)で試作した装置並びに粉体を用いて,歯質上に噴射成膜した.新たに合成したHA粉が,安定して噴射することが可能となり,HA膜の生成も良好で,硬度はHV500程度であることを確認した.さらにSEM並びにTEMによる膜断面の観察を行った結果,均一な断面構造を持つHA粉体が,衝突により一旦数10nmの微粒子に粉砕され,再び再結合して膜形成がされていることが明らかとなった. またそのHA膜は,人工唾液中で放置してその形態変化を観察した結果,膜表面に新たな膜が堆積している状態が観察され,膜厚は実質,増加することが明らかになった.

  52. Mass spectrometric identification of intrinsic growth factors accumulated with Immaterial and reconstruction of highly activated bone regenerative material

    KAMAKURA Shinji, SASANO Yasuyuki, SUZUKI Osamu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2004 - 2006

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    Octacalcium phosphate (OCP) was implanted into the standardized rat calvarial defect. After ten days of implantation, the implants were retrieved and lyophilized, then the protein accumulated with the implants were extracted. The extracted protein was purified and was determined by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Although over 200 types of protein were detected from the experimental groups, it was difficult to identify which protein was directly involved in bone regeneration. During the process of this investigation, we prepared a composite bone morphogenetic protein-2 (BMP-2) combined with OCP (OCP/BMP-2) and implanted into the rat calvarial defect. Because OCP/BMP-2 synergistically enhanced bone regeneration, OCP may be useful as an effective scaffold for BMP-2 (J Biomed Mater Res A 71A : 299-307, 2004). Then, we have engineered OCP-collagen composite (OCP/Col) that was biomimetic components of bone tissue, and implanted. OCP/Col significantly enhanced bone regeneration than OCP alone (J Biomed Mater Res B Appl Biomater 79B : 210-217, 2006), and a patent as "Substitutional scaffold for hard tissue" was applied.. Furthermore, we confirmed that the implantation of OCP/Col in a rat cranial defect enhanced more bone regeneration than β-tricalcium phosphate collagen composite or hydroxyapatite collagen composite, suggesting that OCP/Col would be an effective bone regenerative material (J Biomed Mater Res A in press, 2007).

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