Details of the Researcher

PHOTO

Baiho Kan
Section
Graduate School of Environmental Studies
Job title
Associate Professor
Degree

  • 博士（生命科学）（東北大学）

  • Ｍ．Ｓ．（台湾 国立中興大学）

Profile

1979年台湾生まれ、植物学科卒、生命科学研究科修士・博士号を取得。専門は環境微生物学、遺伝子工学。関心を持って行ってきた研究として、①金属や有機化合物に対する微生物や植物の生物応答の解明、②上記生物機能を活かした環境汚染物質（重金属・有機汚染物）の浄化技術の開発、および③有価金属回収のバイオシステムの開発を行なっている。また最近は④生物間・生物と環境との相互作用の解明、⑤バイオエネルギーの生産についても少し展開している。

Research History 7

  • 2022/04 - Present
    Tohoku University　Graduate School of Environmental Studies Department of Environmental Studies for Advanced Society　Associate Professor

  • 2021/04 - Present
    東北大学大学院　環境科学研究科 寄付講座 環境物質政策学講座（DOWAホールディングス）　（兼担）

  • 2024/05 - 2024/05
    BRAWIJAYA UNIVERSITY　Faculty of Fisheries and Marine Science　Visiting Lecturer

  • 2014/04 - 2023/03
    東北大学大学院　環境科学研究科　助教

  • 2010/04 - 2014/03
    Tohoku Gakuin University　Research Institute for Engineering and Technology

  • 2008/09 - 2010/03
    Ehime University　Center for Marine Environmental Studies

  • 2008/04 - 2008/08
    Tohoku Gakuin University

Show all Show first 5

Education 4

  • Tohoku University

    2005/04 - 2008/03

  • 台湾国立中興大学　大学院　生命科学研究科 博士後期課程

    2004/09 - 2005/03

  • 台湾国立中興大学　大学院　生命科学研究科 修士課程

    2002/09 - 2004/06

  • 台湾国立中興大学　生命科学部　植物学科

    1998/09 - 2002/06

Committee Memberships 8

  • 環境バイオテクノロジー学会　理事

    2023/07 - Present

  • Sustainability　Guest editor of the Special Issue "Green Technologies for Environment, Health and Sustainable Development"

    2022/04 - Present

  • 環境バイオテクノロジー学会　企画委員

    2021/09 - Present

  • International Journal of Environmental Research and Public Health　Guest editor of the Special Issue "Evaluation and Health Impact of Toxic Metals Pollution"

    2020 - Present

  • Frontiers in Microbiology Review Editor

    2013 - Present

  • 生物工学会　和文誌バイオミディア委員

    2023/01 - 2024/12

  • 日本生物工学会第72回（2020）大会実行委員会　実行委員

    2020/03 - 2020/09

  • 環境微生物系学会合同大会２０１７実行委員会　委員

    2016/04 - 2017/08

Show all ︎Show first 5

Professional Memberships 7

  • 資源・素材学会

    - Present

  • Asian Federation of Biotechnology

  • American Society for Microbiology

  • 環境バイオテクノロジー学会

  • 日本生物工学会

  • 日本農芸化学会

  • Japan Society on Water Environment

︎Show all ︎Show first 5

Research Interests 9

  • ファイトレメディエーション

  • 環境バイオテクノロジー

  • Environmental purification

  • Environmental microbiology

  • Biorecovery

  • bioremediation

  • Plant-microbe interaction

  • Genetic engineering

  • Microbial Engineering

Research Areas 4

  • Energy / Earth resource engineering, energy science /

  • Social infrastructure (civil Engineering, architecture, disaster prevention) / Civil engineering (environmental systems) / Environmental biotechnology

  • Environmental science/Agricultural science / Biological resource conservation /

  • Environmental science/Agricultural science / Environmental load reduction/restoration technology /

Awards 14

  1. 優秀ポスター賞

    2024/05　環境バイオテクノロジー学会　生物による金属資源リサイクルのためのニッケル吸着組換え酵母の特徴付け

  2. 優秀ポスター賞

    2024/05　環境バイオテクノロジー学会　Pseudomonas moorei m318 株とその産生する環状ジペプチドの植物成長促進作用

  3. 第6回「バイオインダストリー奨励賞」

    2022/10　一般財団法人 バイオインダストリー協会　生物学的環境技術の効率化に寄与する生物間相互作用の解明と応用展開

  4. 資生堂 女性研究者サイエンスグラント第 15 回

    2022/07　株式会社 資生堂　循環型社会の実現に向けた生物学的金属応答機能の活用

  5. 第5回紫千代萩賞

    2022/03　国立大学法人 東北大学　植物・微生物による環境浄化機構の解明および有効利用の研究

  6. 第2回野本賞

    2021/09　日本微生物学連盟　環境調和型バイオテクノロジーの創出を目指した生物機能と生物間作用の解明

  7. 奨励賞

    2020/07　環境バイオテクノロジー学会　微生物・植物による環境汚染浄化機構の解明とその応用に関する研究

  8. 若手優秀講演賞

    2019/07　第56回アイソトープ・放射線研究発表会　重金属高蓄積植物ハクサンハタザオ体内におけるカドミウムの吸収過程の追跡

  9. The best oral presentation award

    2018/10　15th International Phytotechnology Conference　Enhancement or Inhibition: Understanding the role of plant root exudates in bacterial polycyclic aromatic hydrocarbons degradation using sudangrass

  10. The best poster presentation award

    2018/06　Plant-induced variability in bacterial PAHs degradation in hydroponic culture of sudangrass

  11. 第９回研究奨励賞

    2018/03　NPO法人環境エネルギー技術研究所　植物・微生物による効果的なヒ素除去機構の解明と汚染現場に適用するための技術提案

  12. 第64回日本生物工学会 トピックス集

    2012/10　日本生物工学会　ヒ素高蓄積植物根圏の細菌相の解析と植物によるヒ素蓄積機構に関する研究

  13. The best oral presentation award

    2009/09　Interdisciplinary Symposium “Biological Responses to Chemical Contaminants: from Molecular to Community Level”　Distribution of mercury resistance determinants in highly mercury polluted area of Taiwan

  14. Excellent poster

    2004/10　International Symposium of Environmental Biotechnologies on Bioenergy and Bioremediation　Molecular Characterization of a Group II Intron Identified in Transposon TnMERI1 of Bacillus megaterium MB1

Show all ︎Show 5

Papers 96

  1. Production of lipase and extracellular polymeric substances by the lipid-degrading bacterium Burkholderia arboris strain JYK2 in response to different substrates Peer-reviewed

    Jiyu Huang, Mei-Fang Chien, Hernando P. Bacosa, Chihiro Inoue

    World Journal of Microbiology and Biotechnology　41　(6)　2025/06/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s11274-025-04432-5 　

    ISSN： 0959-3993

    eISSN： 1573-0972

    More details Close

    Abstract Characterization of lipid-degrading microorganisms and understanding their metabolite production mechanisms are essential for enhancing biodegradation efficiency of lipid-rich wastewater. In this study, we isolated Burkholderia arboris strain JYK2, which demonstrated lipid degradation at 46.29 mg/(l • h) with lipase activity reaching 59.92 U/ml when lipid was provided as the sole carbon source. Experimental results revealed that strain JYK2 secretes lipase in media containing lipids and fatty acids but not in glycerol-containing media, a phenomenon likely attributable to quorum sensing mechanisms. Furthermore, extracellular polymeric substances (EPS) were produced in the presence of lipids and fatty acids. Compositional analysis showed that EPS primarily consisted of proteins (approximately 50%) and polysaccharides (approximately 25%). This protein-rich characteristic conferred high hydrophobicity to the EPS, contributing to its lipase adsorption capacity as verified in this study. Additionally, biosurfactant activity was detected in the EPS produced by JYK2. These functions collectively enhance substrate utilization by microorganisms and promote lipid biodegradation. However, minimal EPS production was observed at low fatty acid concentrations, suggesting that the EPS production mechanism cannot be solely attributed to quorum sensing. This study provides insights into the interactions between lipid-degrading bacteria, lipase production, and EPS functionality, which are crucial for optimizing biological treatment of lipid-rich wastewater. Graphical Abstract

  2. Navigating the Depths: A Comprehensive Review of 40 Years of Marine Oil Pollution Studies in the Philippines (1980 to 2024) Peer-reviewed

    Hernando P. Bacosa, Jill Ruby L. Parmisana, Nur Inih U. Sahidjan, Joevin Mar B. Tumongha, Keana Aubrey A. Valdehueza, Jay Rumen U. Maglupay, Andres Philip Mayol, Chin-Chang Hung, Marianne Faith Martinico-Perez, Kozo Watanabe, Mei-Fang Chien, Chihiro Inoue

    Water　17　(11)　1709-1709　2025/06/04

    Publisher: MDPI AG

    DOI： 10.3390/w17111709 　

    eISSN： 2073-4441

    More details Close

    This review synthesizes four decades (1980–2024) of marine oil spill research in the Philippines, analyzing 80 peer-reviewed publications sourced from Scopus, Web of Science, Clarivate, and Google Scholar. Findings show that oil spill research activity spikes after major spills, particularly the 2006 Guimaras incident, which accounts for over half of the reviewed studies and were mostly concentrated in the field of biology, followed by social sciences. Mangroves are the most studied as they are the widely affected ecosystem in the Philippines. Despite the number of published articles on oil spills in the Philippines, only the major events were emphasized, and small-scale spills remain under documented. Research on small-scale oil spills and the country’s two recent big oil spills (Mindoro Oil Spill and Manila Bay Oil Spill), particularly in a country’s environmentally sensitive areas, must be conducted in collaboration with academic institutions and relevant stakeholders to gain a deeper understanding and formulate appropriate countermeasures in the event of future spills. The review also highlights limited application of advanced techniques such as hydrocarbon fingerprinting, geospatial analysis, and next-generation DNA sequencing, limiting comprehensive assessments of oil fate and ecological effects. Addressing these gaps through interdisciplinary collaboration is critical to improving oil spill response, environmental management, and policy formulation in the Philippines’ complex archipelagic setting.

  3. Enhanced Molybdenum Recovery Achieved by a Complex of Porous Material-Immobilized Surface-Engineered Yeast in Development of a Sustainable Biosorption Technology Peer-reviewed

    Thiti Jittayasotorn, Kentaro Kojima, Audrey Stephanie, Kaho Nakamura, Hernando P. Bacosa, Kengo Kubota, Masanobu Kamitakahara, Chihiro Inoue, Mei-Fang Chien

    Microorganisms　13　(5)　1034-1034　2025/04/30

    Publisher: MDPI AG

    DOI： 10.3390/microorganisms13051034 　

    eISSN： 2076-2607

    More details Close

    Molybdenum (Mo) is a critical industrial metal valued for its corrosion resistance and strength-enhancing properties. However, increasing demand necessitates more efficient and sustainable recovery methods. Bio-recovery of Mo by biosorption is a promising resolution, especially by the use of surface-engineered microbes that express metal binding proteins on its cell surface. This study investigates the potential of Saccharomyces cerevisiae strain ScBp6, which displays a molybdate-binding protein (ModE) on its cell surface, immobilized on porous materials. Our findings reveal that polyurethane sponges (PS) significantly outperform ceramic materials in yeast immobilization, entrapping 1.76 × 107 cells per sponge compared to 1.70 × 106 cells per ceramic cube. Furthermore, the yeast–PS complex demonstrated superior Mo adsorption, reaching 2.16 pg Mo per yeast cell under 10 ppm Mo conditions, comparable to free yeast cells (1.96 pg Mo per yeast cell). These results establish PS as an effective and scalable platform for Mo recovery, offering high biosorption efficiency, reusability, and potential for industrial wastewater treatment applications.

  4. Effects of Wood Ash Fertilizer on Element Dynamics in Soil Solution and Crop Uptake Peer-reviewed

    Chuanzhen Jian, Toru Hamamoto, Chihiro Inoue, Mei-Fang Chien, Hiroshi Naganuma, Takehito Mori, Akihiro Sawada, Masafumi Hidaka, Hiroyuki Setoyama, Tomoyuki Makino

    Agronomy　15　(5)　1097-1097　2025/04/30

    Publisher: MDPI AG

    DOI： 10.3390/agronomy15051097 　

    eISSN： 2073-4395

    More details Close

    Wood ash, a byproduct of woody biomass power generation, has potential as an alternative K fertilizer due to its high K content and pH-raising properties. However, concerns remain about heavy metal contaminants like Cr and the limited understanding of element dynamics in soil–solution–crop systems after wood ash’s application. This study examined the effects of 1% (w/w) wood ash on element dynamics and komatsuna (Brassica rapa var. perviridis) uptake in low-K soil through a pot experiment. XRD was used to analyze mineral composition, SEM-EDS to observe surface and elemental properties, and XANES to examine Cr speciation in wood ash. Soil solution analysis covered macro- and micronutrients, heavy metals, anions, pH, and DOC, while crop element concentrations and aboveground dry weight were also quantified. The chemical speciation of Cu and Cr in a soil solution was modeled using Visual MINTEQ. Wood ash significantly increased K concentrations (from 17 mg/L to 650 mg/L) in the soil solution, along with Ca, Mg, P, and Mo, while reducing Ni, Mn, Zn, and Cd levels. Komatsuna K uptake surged from 123 mg/kg to 559 mg/kg, leading to a 3.31-fold biomass increase. Notably, the Cd concentration in the crops dropped significantly from 0.709 to 0.057 mg/kg, well below the Codex standard of 0.2 mg/kg. Although Cu and Cr concentrations rose in the soil solution, crop uptake remained low due to >99% complexation with fulvic acid, as confirmed by Visual MINTEQ modeling. This study confirms that wood ash is an effective K fertilizer, but emphasizes the need for risk mitigation strategies to ensure safe and sustainable agricultural application.

  5. Effect of oxidation treatment and UV irradiation of titanium on surface properties and yeast adhesion Peer-reviewed

    Masaki Umetsu, Ryota Sekiai, Mei-Fang Chien, Shun Yokoyama, Masanobu Kamitakahara

    Journal of Asian Ceramic Societies　1-10　2025/04/15

    Publisher: Informa UK Limited

    DOI： 10.1080/21870764.2025.2492407 　

    eISSN： 2187-0764

  6. Unraveling tissue-specific molecular mechanisms orchestrating arsenic response processes in Pteris vittata through transcriptomic analysis Peer-reviewed

    Shujun Wei, Ryota Moriuchi, Christine Dwi A.P. Wiyono, Chihiro Inoue, Mei-Fang Chien

    Ecotoxicology and Environmental Safety　2025/04

    DOI： 10.1016/j.ecoenv.2025.118059 　

  7. Mesoplastics: A Review of Contamination Status, Analytical Methods, Pollution Sources, Potential Risks, and Future Perspectives of an Emerging Global Environmental Pollutant Peer-reviewed

    Dioniela Mae C. Ellos, Mei-Fang Chien, Chihiro Inoue, Haruka Nakano, Atsuhiko Isobe, Deo Florence L. Onda, Kozo Watanabe, Hernando P. Bacosa

    Toxics　2025/03/20

    DOI： 10.3390/toxics13030227 　

  8. Complete genome sequence of a carbon tetrachloride-degrading bacterium, Pseudomonas sp. strain Stari2 Peer-reviewed

    Leonardo Stari, Thiti Jittayasotorn, Chihiro Inoue, Mei-Fang Chien

    Microbiology Resource Announcements　2025/03/11

    DOI： 10.1128/mra.00958-24 　

  9. New insights into immobilized bacterial systems for removal of heavy metals from wastewater Peer-reviewed

    Jhariya, U., Chien, M.-F., Umetsu, M., Kamitakahara, M.

    International Journal of Environmental Science and Technology　22　(9)　2025/02/10

    DOI： 10.1007/s13762-025-06369-6 　

    ISSN： 1735-2630 1735-1472

  10. Exploring Cd tolerance and detoxification strategies of Arabidopsis halleri ssp. gemmifera under high cadmium exposure Peer-reviewed

    Sari, S.H.J., Chien, M.-F., Inoue, C.

    International Journal of Phytoremediation　27　(7)　2025/01/29

    DOI： 10.1080/15226514.2025.2456678 　

    ISSN： 1549-7879 1522-6514

  11. Complete genome sequence of Pseudomonas moorei strain m318 isolated from rhizosphere of Pteris multifida in high-arsenic-content soil Peer-reviewed

    Ning Han, ChongYang Yang, Ying-Ning Ho, Ryota Moriuchi, Che-Chun Chen, Chihiro Inoue, Mei-Fang Chien

    Microbiology Resource Announcements　2024/12/12

    DOI： 10.1128/mra.00774-24 　

  12. Synergized Effects of Amino Acids and NaCl to Enhance Silicate Mineral Dissolution in Aqueous Environments for Efficient Atmospheric CO2 Removal Peer-reviewed

    Jiajie Wang, Sena Kikuchi, Noriyoshi Tsuchiya, Yoshinori Sato, Mei-Fang Chien, Noriaki Watanabe

    Environmental Science & Technology　2024/12/02

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.est.4c07526 　

    ISSN： 0013-936X

    eISSN： 1520-5851

  13. Effective selenate removal using pH modulated synthesis of biogenic jarosite: Comparative insight with non-biogenic jarosite and biogenic schwertmannite Peer-reviewed

    Upasana Jhariya, Mei-Fang Chien, Masaki Umetsu, Masanobu Kamitakahara

    Journal of Hazardous Materials　480　136256-136256　2024/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.jhazmat.2024.136256 　

    ISSN： 0304-3894

  14. CoNi layered double hydroxides grown on Ag nanowires for high-efficient nitrate-to-ammonia conversion Peer-reviewed

    Xufeng Rao, Shuoshuo Feng, Yanhong Zou, Linlin Wang, Mei-fang Chien, Chihiro Inoue, Yuyu Liu

    Separation and Purification Technology　354　129286-129286　2024/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.seppur.2024.129286 　

    ISSN： 1383-5866

  15. Effects of temperature on plant growth and arsenic removal efficiency of Pteris vittata in purifying arsenic-contaminated water in winter: A two-year year-round field study Peer-reviewed

    Yi Huang-Takeshi Kohda, Keisuke Miyauchi, Farzana Rahman, Haruki Naruse, Mitsuaki Mito, Nobuyuki Kitajima, Mei-Fang Chien, Ginro Endo, Chihiro Inoue

    Chemosphere　362　142902-142902　2024/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.chemosphere.2024.142902 　

    ISSN： 0045-6535

  16. データサイエンスが紐解く複合生物系による環境修復機構とそこから見える課題 Invited Peer-reviewed

    簡梅芳, 韓凝, 工藤宏史, 井上千弘

    環境バイオテクノロジー学会誌　24　(1)　83-89　2024/07

    DOI： 10.50963/jenvbio.24.1_83 　

  17. Bayesian network highlights the contributing factors for efficient arsenic phytoextraction by Pteris vittata in a contaminated field Peer-reviewed

    Hiroshi Kudo, Ning Han, Daiki Yokoyama, Tomoko Matsumoto, Mei-Fang Chien, Jun Kikuchi, Chihiro Inoue

    Science of The Total Environment　899　165654-165654　2023/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.scitotenv.2023.165654 　

    ISSN： 0048-9697

  18. A porous Co3O4-carbon paper electrode enabling nearly 100% electrocatalytic reduction of nitrate to ammonia Peer-reviewed

    Xufeng Rao, Jiaying Yan, Koji Yokoyama, Xiaolin Shao, Chihiro Inoue, Mei-Fang Chien, Yuyu Liu

    Materials Reports: Energy　100216-100216　2023/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.matre.2023.100216 　

    ISSN： 2666-9358

  19. A microbial consortium led by a novel Pseudomonas species enables degradation of carbon tetrachloride under aerobic conditions Peer-reviewed

    Stari, L., Tusher, T.R., Inoue, C., Chien, M.-F.

    Chemosphere　319　137988-137988　2023/04

    Publisher: Elsevier BV

    DOI： 10.1016/j.chemosphere.2023.137988 　

    ISSN： 1879-1298 0045-6535

  20. Temperature Dependence of Metals Accumulation and Removal Kinetics by Arabidopsis halleri ssp. gemmifera Peer-reviewed

    Hiroshi Kudo, Zhaojie Qian, Chihiro Inoue, Mei-Fang Chien

    Plants　12　(4)　877-877　2023/02/15

    Publisher: MDPI AG

    DOI： 10.3390/plants12040877 　

    eISSN： 2223-7747

    More details Close

    Cadmium (Cd), which is present in zinc (Zn) ore, is a toxic metal and causes contamination globally. Phytoremediation is a promising technology for the remediation of sites with low and moderate contamination. Temperature is an important factor in phytoremediation because it has an impact on both plant biomass and the accumulation of heavy metals. However, little is known about the influence of temperature on heavy metal accumulation by the Cd and Zn hyperaccumulator Arabidopsis halleri ssp. gemmifera. The effect of temperature on the distribution of Cd and Zn in A. halleri ssp. gemmifera and the mechanism of metal removal from solution were investigated in this study. Our results showed that the temperature dependence of the distribution of Cd and Zn in the plant was different, which may suggest that the mechanisms of xylem loading were different between Cd and Zn. Although Cd and Zn have partially similar transport pathways, the removal kinetics based on the first-order reaction rate constant revealed that the temperature which maximized rate of absorption was different between Cd and Zn. This study suggests a potential for efficient Cd phytoextraction using A. halleri ssp gemmifera in Cd and Zn co-existing environments.

  21. The benefit of the Arabidopsis halleri ssp. gemmifera root exudate in cadmium extraction from the cadmium contaminated soil Peer-reviewed

    Ariyanti, A.L., Chien, M.-F., Inoue, C.

    Journal of Degraded and Mining Lands Management　10　(2)　4107-4107　2023/01/01

    Publisher: Faculty of Agriculture, Brawijaya University

    DOI： 10.15243/jdmlm.2023.102.4107 　

    ISSN： 2502-2458 2339-076X

    eISSN： 2502-2458

  22. Cadmium and zinc accumulation behaviour of hyperaccumulator Arabidopsis halleri ssp. gemmifera in the hydroponic system Peer-reviewed

    Sari, S.H.J., Chien, M.-F., Inoue, C.

    Journal of Degraded and Mining Lands Management　10　(2)　4155-4155　2023/01/01

    Publisher: Faculty of Agriculture, Brawijaya University

    DOI： 10.15243/jdmlm.2023.102.4155 　

    ISSN： 2502-2458 2339-076X

    eISSN： 2502-2458

  23. Efficient biodegradation of 1,4-dioxane commingled with additional organic compound: Role of interspecies interactions within consortia Peer-reviewed

    Tusher, T.R., Inoue, C., Chien, M.-F.

    Chemosphere　308　136440-136440　2022/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.chemosphere.2022.136440 　

    ISSN： 1879-1298 0045-6535

  24. Recent progress in noble metal electrocatalysts for nitrogen-to-ammonia conversion Peer-reviewed

    Rao, X., Liu, M., Chien, M., Inoue, C., Zhang, J., Liu, Y.

    Renewable and Sustainable Energy Reviews　168　112845-112845　2022/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.rser.2022.112845 　

    ISSN： 1879-0690 1364-0321

  25. Second-generation bioethanol production from phytomass after phytoremediation using recombinant bacteria-yeast co-culture Peer-reviewed

    Tanmoy Roy Tusher, Jui-Jen Chang, Maria Ita Saunivalu, Sosuke Wakasa, Wen-Hsiung Li, Chieh-Chen Huang, Chihiro Inoue, Mei-Fang Chien

    Fuel　326　124975-124975　2022/10

    Publisher: Elsevier {BV}

    DOI： 10.1016/j.fuel.2022.124975 　

    ISSN： 0016-2361

  26. Subcellular localization and chemical speciation of Cd in Arabidopsis halleri ssp. gemmifera to reveal its hyperaccumulating and detoxification strategies Peer-reviewed

    Sari, S.H.J., Chien, M.-F., Inoue, C.

    Environmental and Experimental Botany　203　105047-105047　2022/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.envexpbot.2022.105047 　

    ISSN： 0098-8472

  27. Rhizospheric plant-microbe synergistic interactions achieve efficient arsenic phytoextraction by Pteris vittata Peer-reviewed

    Chongyang Yang, Ning Han, Chihiro Inoue, Yu-Liang Yang, Hideaki Nojiri, Ying-Ning Ho, Mei-Fang Chien

    Journal of Hazardous Materials　434　128870-128870　2022/07

    Publisher: Elsevier {BV}

    DOI： 10.1016/j.jhazmat.2022.128870 　

    ISSN： 0304-3894

  28. Arsenic uptake by Pteris vittata in a subarctic arsenic-contaminated agricultural field in Japan: An 8-year study Peer-reviewed

    Yi Huang-Takeshi Kohda, Ginro Endo, Nobuyuki Kitajima, Kazuki Sugawara, Mei-Fang Chien, Chihiro Inoue, Keisuke Miyauchi

    Science of The Total Environment　831　154830-154830　2022/07

    Publisher: Elsevier {BV}

    DOI： 10.1016/j.scitotenv.2022.154830 　

    ISSN： 0048-9697

  29. Empirical Evidence of Arsenite Oxidase Gene as an Indicator Accounting for Arsenic Phytoextraction by Pteris vittata Peer-reviewed

    Ning Han, Chongyang Yang, Shunya Shimomura, Chihiro Inoue, Mei-Fang Chien

    International Journal of Environmental Research and Public Health　19　(3)　1796-1796　2022/02/04

    Publisher: MDPI AG

    DOI： 10.3390/ijerph19031796 　

    eISSN： 1660-4601

    More details Close

    Arsenic (As) is a toxic semi-metallic element that is ubiquitous in the environment and poses serious human health risks. Phytoextraction by Pteris vittata is considered a low-cost and environmentally friendly approach to treat As-contaminated soil. P. vittata mainly absorbs arsenate thus the bioavailability of As to P. vittata depends on the chemical form of As. Microbial redox of As contributes to the biogeochemical cycling of As, and rhizobacterium-assisted phytoextraction by P. vittata was proposed. In this study, this microbe-assisted phytoextraction was applied to two fields, and the effectiveness of phytoextraction was evaluated. The results revealed that P. vittata was able to grow in temperate and subarctic climate zones. The biomass was influenced by the weather, and the As concentration in plants was dependent on the As content in the soil. The ratio of arsenite oxidase genes (aioA-like genes) to 16S rRNA genes was employed to evaluate the effect of As phytoextraction, and the results exhibited that the ratio was related to the As concentration in P. vittata. Our results showed that arsenite oxidation in the rhizosphere might not be achieved by single-strain inoculation, while this study provided empirical evidence that the rhizospheric aioA-like genes could be an indicator for evaluating the effectiveness of As phytoextraction.

  30. HMA4 and IRT3 as indicators accounting for different responses to Cd and Zn by hyperaccumulator Arabidopsis halleri ssp. gemmifera Peer-reviewed

    Christine Dwi AP. Wiyono, Chihiro Inoue, Mei-Fang Chien

    Plant Stress　2　100042-100042　2021/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.stress.2021.100042 　

    ISSN： 2667-064X

  31. Separation of microplastic from soil by centrifugation and its application to agricultural soil Peer-reviewed

    Guido Grause, Yamato Kuniyasu, Mei-Fang Chien, Chihiro Inoue

    Chemosphere　288　132654-132654　2021/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.chemosphere.2021.132654 　

    ISSN： 0045-6535

  32. 微生物;植物による環境汚染浄化機構の解明とその応用—ヒ素のファイトエキストラクションおよび多環芳香族炭化水素のリゾデグラデーションを例として Invited Peer-reviewed

    簡梅芳, 楊重陽, 魏書君, John Jewish, A. Dominguez, 何攖寧, 井上千弘

    環境バイオテクノロジー学会誌　21　(1)　1-7　2021/09

  33. Influence of low temperature on comparative arsenic accumulation and release by three Pteris hyperaccumulators Peer-reviewed

    Rahman, F., Sugawara, K., Wei, S., Kohda, Y.H.-T., Chien, M.-F., Inoue, C.

    Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering　56　(11)　1179-1188　2021/08

    DOI： 10.1080/10934529.2021.1970443 　

    ISSN： 1532-4117 1093-4529

  34. New evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer Peer-reviewed

    Yi Huang-Takeshi Kohda, Zhaojie Qian, Mei-Fang Chien, Keisuke Miyauchi, Ginro Endo, Nobuo Suzui, Yong-Gen Yin, Naoki Kawachi, Hayato Ikeda, Hiroshi Watabe, Hidetoshi Kikunaga, Nobuyuki Kitajima, Chihiro Inoue

    Scientific Reports　11　(1)　12149-12158　2021/07

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-021-91374-1 　

    eISSN： 2045-2322

    More details Close

    <title>Abstract</title><italic>Pteris vittata</italic> is an arsenic (As) hyperaccumulator plant that accumulates a large amount of As into fronds and rhizomes (around 16,000 mg/kg in both after 16 weeks hydroponic cultivation with 30 mg/L arsenate). However, the sequence of long-distance transport of As in this hyperaccumulator plant is unclear. In this study, we used a positron-emitting tracer imaging system (PETIS) for the first time to obtain noninvasive serial images of As behavior in living plants with positron-emitting 74As-labeled tracer. We found that As kept accumulating in rhizomes as in fronds of <italic>P. vittata</italic>, whereas As was retained in roots of a non-accumulator plant <italic>Arabidopsis thaliana</italic>. Autoradiograph results of As distribution in <italic>P</italic>. <italic>vittata</italic> showed that with low As exposure, As was predominantly accumulated in young fronds and the midrib and rachis of mature fronds. Under high As exposure, As accumulation shifted from young fronds to mature fronds, especially in the margin of pinna, which resulted in necrotic symptoms, turning the marginal color to gray and then brown. Our results indicated that the function of rhizomes in <italic>P. vittata</italic> was As accumulation and the regulation of As translocation to the mature fronds to protect the young fronds under high As exposure.

  35. Isolation and characterization of novel bacteria capable of degrading 1,4-dioxane in the presence of diverse co-occurring compounds Peer-reviewed

    Tusher, T.R., Shimizu, T., Inoue, C., Chien, M.-F.

    Microorganisms　9　(5)　887-900　2021/04/21

    DOI： 10.3390/microorganisms9050887 　

    ISSN： 2076-2607

  36. Expression of PvPht1;3, PvACR2 and PvACR3 during arsenic processing in root of Pteris vittata Peer-reviewed

    Wei, S., Kohda, Y.H.-T., Inoue, C., Chien, M.-F.

    Environmental and Experimental Botany　182　104312-104312　2021/02

    Publisher: Elsevier BV

    DOI： 10.1016/j.envexpbot.2020.104312 　

    ISSN： 0098-8472

  37. ヒ素高蓄積植物モエジマシダ体内におけるヒ素の輸送過程のイメージング

    黄田 毅, 銭 照杰, 簡 梅芳, 井上 千弘, 宮内 啓介, 遠藤 銀郎, 池田 隼人, 菊永 英寿, 渡部 浩司, 鈴井 伸郎, 尹 永根, 河地 有木, 北島 信行

    アイソトープ・放射線研究発表会　1　32　2021

    Publisher: 公益社団法人 日本アイソトープ協会

    DOI： 10.50955/happyokai.1.0_32 　

    eISSN： 2436-4487

    More details Close

    モエジマシダ (Pteris vittata) はヒ素(As)の高蓄積植物であり、乾燥重量当たり最大で20,000 mg/kg Asを蓄積することが知られており、ヒ素で汚染された土壌と水の植物浄化処理(phytoremediation)への応用に期待されている。これまでにモエジマシダにおけるAsの輸送過程は明らかにされていない。そこで本研究ではPETISを利用し74Asを用いて、モエジマシダが生きたままの状態で Asの輸送過程を観測した結果、根から吸収されたAsが根茎部に一度蓄積された後に地上部に輸送されることが明らかになった。

  38. Biomimetic anti-biofouling oil-infused honeycomb films fabricated by breath figures Peer-reviewed

    Hiroya Abe, Ryunosuke Shimura, Hiroshi Yabu, Mei-Fang Chien, Chihiro Inoue

    Polymer Journal　2021

    DOI： 10.1038/s41428-021-00467-1 　

    ISSN： 0032-3896

    eISSN： 1349-0540

  39. Changes during the weathering of polyolefins Peer-reviewed

    Guido Grause, Mei Fang Chien, Chihiro Inoue

    Polymer Degradation and Stability　181　109364　2020/11

    DOI： 10.1016/j.polymdegradstab.2020.109364 　

    ISSN： 0141-3910

  40. Molybdate recovery using immobilized bioengineered Saccharomyces cerevisiae Peer-reviewed

    Stephanie, A., Chien, M.-F., Ikeda, N., Inoue, C.

    Hydrometallurgy　198　105491-105499　2020/10

    DOI： 10.1016/j.hydromet.2020.105491 　

    ISSN： 0304-386X

  41. Potential of biosurfactants’ production on degrading heavy oil by bacterial consortia obtained from tsunami-induced oil-spilled beach areas in Miyagi, Japan Peer-reviewed

    Sandia Primeia, Chihiro Inoue, Mei-Fang Chien

    Journal of Marine Science and Engineering　8　(8)　577-593　2020/07/31

    DOI： 10.3390/JMSE8080577 　

    eISSN： 2077-1312

  42. Long-term effectiveness of microbe-assisted arsenic phytoremediation by Pteris vittata in field trials Peer-reviewed

    Yang, C., Ho, Y.-N., Inoue, C., Chien, M.-F.

    Science of the Total Environment　740　140137-140145　2020

    DOI： 10.1016/j.scitotenv.2020.140137 　

  43. Hydroponic approach to assess rhizodegradation by sudangrass (Sorghum x Peer-reviewed

    John Jewish, A. Dominguez, Chihiro Inoue, Mei-Fang Chien

    Journal of Hazardous Materials　387　121695-121705　2020

  44. Hydroponic approach to assess rhizodegradation by sudangrass (Sorghum x drummondii) reveals pH- and plant age-dependent variability in bacterial degradation of polycyclic aromatic hydrocarbons (PAHs) Peer-reviewed

    Dominguez, J.J.A., Inoue, C., Chien, M.-F.

    Journal of Hazardous Materials　387　121695-121605　2020

    DOI： 10.1016/j.jhazmat.2019.121695 　

  45. Cupriavidus basilensis strain r507, a toxic arsenic phytoextraction facilitator, potentiates the arsenic accumulation by Pteris vittata Peer-reviewed

    Yang, C., Ho, Y.-N., Makita, R., Inoue, C., Chien, M.-F.

    Ecotoxicology and Environmental Safety　190　110075-110082　2020

    DOI： 10.1016/j.ecoenv.2019.110075 　

  46. Enrichment and analysis of stable 1,4-dioxane- degrading microbial consortia consisting of novel dioxane-degraders Peer-reviewed

    Tusher, T.R., Shimizu, T., Inoue, C., Chien, M.-F.

    Microorganisms　8　(1)　50-60　2020

    DOI： 10.3390/microorganisms8010050 　

  47. A multifunctional rhizobacterial strain with wide application in different ferns facilitates arsenic phytoremediation Peer-reviewed

    Chongyang Yang, Ying-Ning Ho, Ryota Makita, Chihiro Inoue, Mei-Fang Chien

    Science of the Total Environment　712　134504-134514　2020

    DOI： 10.1016/j.scitotenv.2019.134504 　

    ISSN： 0048-9697

    eISSN： 1879-1026

  48. Phosphorus- And Iron-Deficiency Stresses Affect Arsenic Accumulation and Root Exudates in Pteris vittata Peer-reviewed

    Yang, C., Chien, M.-F., Ho, Y.-N., Inoue, C.

    International Journal of Environmental Science and Development　10　(12)　430-434　2019/11

    DOI： 10.18178/ijesd.2019.10.12.1211 　

  49. Identification of a novel arsenic resistance transposon nested in a mercury resistance transposon of bacillus sp. Mb24 Invited Peer-reviewed

    Chien, M.-F., Ho, Y.-N., Yang, H.-E., Narita, M., Miyauchi, K., Endo, G., Huang, C.-C.

    Microorganisms　7　(11)　566-576　2019/11

    DOI： 10.3390/microorganisms7110566 　

  50. Overexpression of PvPht1;3, PvACR2, and PvACR3 in yeast toward an understanding of arsenic hyperaccumulator Pteris vittata Peer-reviewed

    Shu Jun Wei, Mei-Fang Chien, J.J.Chang, Y.J. Lin, W. H. Li, Chihiro Inoue

    2019/09

  51. Investigating the Influence of Temperature on Cadmium and Zinc Absorption of Arabidopsis halleri ssp. Gemmiferain Hydroponics Peer-reviewed

    Zao Jie Qian, Yi Huang-Takeshi Kohda, Mei-Fang Chien, Nobuyuki Kitajima, A. Kanayama, Chihiro Inoue

    Proceeding of 16th International Phytotechnology Conference　226　2019/09

  52. A field application of multi-functional rhizobacterium for elevating arsenic phytoremediation by Pteris vittata Peer-reviewed

    ChonYang Yang, Ryota Makita, Mei-Fang Chien, Chihiro Inoue

    Proceeding of 16th International Phytotechnology Conference　73　2019/09

  53. Assessing HMA4, IRT3, NRAMP5 as Potential Cadmium Resistance Genes in a Hyperaccumulator Plant Arabidopsis halleri ssp. Gemmifera Peer-reviewed

    Christine Dwi, A. Wiyono, Mei-Fang Chien, Chihiro Inoue

    Proceeding of 16th International Phytotechnology Conference　61　2019/09

  54. A challenging to transfer biomass after phytoremediation to biofuel Peer-reviewed

    Mei-Fang. Chien, Sousuke Wakasa, Jui-Jen Chang, Yu-Ju. Lin, Wen-Hsing. Li, Chihiro Inoue

    Proceeding of 16th International Phytotechnology Conference　160　2019/09

  55. MerB3, an organomercurial lyase of Bacillus as an antidote against organomercurial poisoning Peer-reviewed

    Mei-Fang Chien, Ying-Ning Ho, Hui-Tzu Lin, Kuo-Hsing Lin, Ginro Endo, Chieh-Chen Huang

    Journal of Environmental Biotechnology　19　(1)　73-80　2019/07

  56. Comparative geochemical evaluation of toxic metals pollution and bacterial communities of industrial effluent tributary and a receiving estuary in Nigeria. International-journal Peer-reviewed

    Ganiyu O Oyetibo, Keisuke Miyauchi, Yi Huang, Wakako Ikeda-Ohtsubo, Mei-Fang Chien, Matthew O Ilori, Olukayode O Amund, Ginro Endo

    Chemosphere　227　638-646　2019/07

    DOI： 10.1016/j.chemosphere.2019.04.048 　

    More details Close

    Toxic metals/metalloid contaminations of estuarine sediments due to compromised tributaries arouse significant interest in studying bacterial community that triggers natural attenuation processes. Geo-accumulation index (Igeo), contamination factor (CF), pollution load index (PLI), and Hakanson potential ecological risk index (RI) as a sum of risk factors (Er) were used to quantify toxic metal/metalloid-pollution status of Lagos Lagoon (2W) and 'Iya-Alaro' tributary (4W) sediments in comparison with pristine 'Lekki Conservation Centre' sediment (L1-B). Bacteriology of the ecosystems was based on culture-independent analyses using pyrosequencing. 2W and 4W were extremely contaminated with mercury (Igeo > 7), whereas, cadmium contamination was only observed in 4W. The two ecosystems were polluted with toxic metal based on PLI, where mercury (Er = 2900 and 1900 for 4W and 2W, respectively) posed very high ecological risks. Molecular fingerprinting revealed that Proteobacteria, Firmicutes, and Acidobacteria predominately contributed the 20 most abundant genera in the two ecosystems. The 240 and 310 species present in 2W and 4W, respectively, but absent in L1-B, thrive under the metal concentrations in the polluted hydrosphere. Whereas, the 58,000 species missing in 2W and 4W but found in L1-B would serve as indicators for systems impacted with metal eco-toxicity. Despite toxic metal pollution of the ecosystems understudied, bacterial communities play vital roles in self-recovery processes occurring in the hydrosphere.

  57. Enhanced degradation of polycyclic aromatic hydrocarbons (PAHs) in the rhizosphere of sudangrass (Sorghum × drummondii) Peer-reviewed

    Dominguez, J.J.A., Bacosa, H.P., Chien, M.-F., Inoue, C.

    Chemosphere　234　789-795　2019/06

    DOI： 10.1016/j.chemosphere.2019.05.290 　

  58. Efficient nitrate removal from water using selected cathodes and Ti/PbO2 anode: Experimental study and mechanism verification. Peer-reviewed

    Xufeng Rao, Xiaolin Shao, Jie Xua, Jin Yi, Jinli Qiao, Qingyu Li, Hongqiang Wang, Mei-Fang Chien, Chihiro Inoue, Yuyu Liu, Jiujun Zhang

    Separation and Purification Technology　216　158-165　2019

    DOI： 10.1016/j.seppur.2019.02.009 　

    ISSN： 1383-5866

    eISSN： 1873-3794

  59. Arsenic, lead and cadmium removal potential of Pteris multifida from contaminated water and soil Peer-reviewed

    Rahman, F., Sugawara, K., Huang, Y., Chien, M.-F., Inoue, C.

    International Journal of Phytoremediation　20　(12)　1187-1193　2018/12

    DOI： 10.1080/15226514.2017.1375896 　

  60. Enhancement of inhibition: understanding the role of plant root exudates in bacterial polycyclic aromatic hydrocarbons degradation using sudangrass Peer-reviewed

    J.A. Dominguez, M.F. Chien, C. Inoue

    Proceedings of 15th International Phytotechnologies Conference　2018/09

  61. Gene expression of a phosphate transporter and an arsenate reductase in Pteris vittata during arsenic uptake Peer-reviewed

    Shujun Wei, Mei-Fang Chien, Yi Huang-Takeshi Kohda, Chihiro Inoue

    Proceedings of 15th International Phytotechnologies Conference　2018/09

  62. Efficient arsenic phytoremediation by co-culture of Pterix multifida with a multifunctional rhizobiacterium Peer-reviewed

    Chongyang Yang, Mei-Fang Chien, Chihiro Inoue

    Proceedings of 15th International Phytotechnologies Conference　2018/09

  63. Cadmium removal from mine drainage using Arabidopsis halleri ssp. gemmifera through hydroponic culture Peer-reviewed

    Zhaojie Qian, Mei-Fang Chien, YiHuang-Takeshi Kohda, Nobuyuki Kitajima, Kentaro Hayashi, Akihiro Kanayama, Chihiro Inoue

    Proceedings of 15th International Phytotechnologies Conference　2018/09

  64. Further potential of phytoremediation drawed by rhizospheric microoragnisms Invited Peer-reviewed

    2　(7)　682-683　2018/07

  65. Analysis of stable 1,2-dichlorobenzene-degrading enrichments and two newly isolated degrading strains, Acidovorax sp sk40 and Ralstonia sp sk41 Peer-reviewed

    Ge Cui, Mei-Fang Chien, Koichi Suto, Chihiro Inoue

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY　101　(17)　6821-6828　2017/09

    DOI： 10.1007/s00253-017-8406-2 　

    ISSN： 0175-7598

    eISSN： 1432-0614

  66. WBC11 as a Potential Gene for Cadmium Accumulation/Transportation in Arabidopsis halleri ssp. gemmifera Peer-reviewed

    C. D. A. Wiyono, M.-F. Chien, C. Inoue

    Proceedings of 14th International Phytotechnologies Conference　31-31　2017/09

  67. Comparative analysis of arsenate reductase gene expression in two arsenic hyperaccumulator Pteris ferns Peer-reviewed

    Shujun Wei, Mei-Fang Chien, Chihiro Inoue

    Proceedings of 14th International Phytotechnologies Conference　31-31　2017/09

  68. Adsorption of Cd and Zn to the root of Arabidopsis halleri ssp. gemmifera Peer-reviewed

    Z.-J. Qian, Y. Huang, M.-F. Chien, C. Inoue

    Proceedings of 14th International Phytotechnologies Conference　68-68　2017/09

  69. VISUALIZATION OF ZINC TRANSLOCATION DYNAMIC IN HYPERACCUMULATOR ARABIDOPSIS HALLERI SSP. GEMMIFERA AND NON-ACCUMULATOR ARABIDOPSIS THALIANA Peer-reviewed

    Y. Huang, Z. Qian, M.-F. Chien, K. Sugawara, N. Suzui, Y. Yin, N. Kawachi, H. Watabe, N. Kitajima, C. Inoue

    Proceedings of 14th International Phytotechnologies Conference　43-43　2017/09

  70. Influence of Temperature on Arsenic Uptake by Pteris cretica from Hydroponic Solution Peer-reviewed

    F. Rahman, K. Sugawara, Y. Huang, M.-F. Chien, C. Inoue

    Proceedings of 14th International Phytotechnologies Conference　76-76　2017/09

  71. Biodegradation of crude oil and phenanthrene by heavy metal resistant Bacillus subtilis isolated from a multi-polluted industrial wastewater creek Peer-reviewed

    Ganiyu Oladunjoye Oyetibo, Mei-Fang Chien, Wakako Ikeda-Ohtsubo, Hitoshi Suzuki, Oluwafemi Sunday Obayori, Sunday Adekunle Adebusoye, Matthew Olusoji Ilori, Olukayode Oladipo Amund, Ginro Endo

    INTERNATIONAL BIODETERIORATION & BIODEGRADATION　120　143-151　2017/05

    DOI： 10.1016/j.ibiod.2017.02.021 　

    ISSN： 0964-8305

    eISSN： 1879-0208

  72. Biotechnological remedies for the estuarine environment polluted with heavy metals and persistent organic pollutants Peer-reviewed

    Ganiyu Oladunjoye Oyetibo, Keisuke Miyauchi, Yi Huang, Mei-Fang Chien, Matthew Olusoji Ilori, Olukayode Oladipo Amund, Ginro Endo

    INTERNATIONAL BIODETERIORATION & BIODEGRADATION　119　614-625　2017/04

    DOI： 10.1016/j.ibiod.2016.10.005 　

    ISSN： 0964-8305

    eISSN： 1879-0208

  73. Construction of a cell surface engineered yeast aims to selectively recover molybdenum, a rare metal Peer-reviewed

    Mei-Fang Chien, Naoya Ikeda, Kengo Kubota, Chihiro Inoue

    Solid State Phenomena　262　421-424　2017

    Publisher: Trans Tech Publications Ltd

    DOI： 10.4028/www.scientific.net/SSP.262.421 　

    ISSN： 1662-9779

  74. Phytofiltration potential of Pteris multifida for accumulating As, Pb, Cd and Se from mixed metal solution Peer-reviewed

    Farzana Rahman, Kazuki Sugawara, Mei-Fang Chien, Chihiro, Inoue

    Proceedings of 13th International Phytotechnologies Conference　50　2016/09

  75. Analysis of bacteria in rhizosphere of two arsenic hyperaccumulators and their effects on phytoremediation of arsenic Peer-reviewed

    Ryota Makita, Mei-Fang Chien, Hirofumi Nagayama, Chihiro, Inoue

    Proceedings of 13th International Phytotechnologies Conference,　117　2016/09

  76. Phenotypic and genotypic analysis of arsenic accumulation and transformation in three Pteris ferns Peer-reviewed

    Shujun Wei, Mei-Fang Chien, Kazuki Sugawara, Chihiro Inoue

    Proceedings of 13th International Phytotechnologies Conference　83　2016/09

  77. Mercury resistance transposons in Bacilli strains from different geographical regions Peer-reviewed

    Kazuaki Matsui, Satoshi Yoshinami, Masaru Narita, Mei-Fang Chien, Le T. Phung, Simon Silver, Ginro Endo

    FEMS MICROBIOLOGY LETTERS　363　(5)　fnw013　2016/03

    DOI： 10.1093/femsle/fnw013 　

    ISSN： 0378-1097

    eISSN： 1574-6968

  78. Study on As uptake and rhizobacteria of two As hyperaccumulators forward to As phytoremediation Peer-reviewed

    Mei-Fang Chien, Ryota Makita, Kazuki Sugawara, Chihiro Inoue

    Advanced Materials Research　1130　568-572　2015/11

    DOI： 10.4028/www.scientific.net/AMR.1130.568 　

  79. Characterization of rhizobacteria isolated from arsenic hyperaccumulator ferns Peer-reviewed

    Mei-Fang Chien, Ryota Makita, Hirofumi Nagayama, Kazuki Sugawara, Chihiro Inoue

    Proceedings of 12th International Phytotechnologies Conference　107　2015/09

  80. Evaluation of arsenic behavior in temperate-zone plant, Pteris multifida Peer-reviewed

    Kazuki Sugawara, Mei-Fang Chien, Chihiro Inou

    Proceedings of 12th International Phytotechnologies Conference　137　2015/09

  81. Study on As uptake and removal by As hyper-accumulator Pteris vittata and it’s rhizosphere bacteria Peer-reviewed

    Mei Fang Chien, Kazuki Obata, Yi Huang, Keisuke Miyauchi, Ginro Endo, Chihiro Inoue

    Proceedings of 11th International Phytotechnologies Conference　147　2014/09

  82. ヒ素高蓄積植物による土壌ヒ素の吸収・除去と土壌微生物の亜ヒ酸酸化に関する研究 Peer-reviewed

    簡 梅芳, 小畑和貴, 黄 毅, 宮内啓介, 遠藤銀朗

    土木学会論文集　69　(7)　III9-III15　2013/11

    Publisher: Japan Society of Civil Engineers

    DOI： 10.2208/jscejer.69.III_9 　

    ISSN： 2185-6648

    More details Close

    Arsenic hyper-accumulators such as Pteris vittata have been known applicable to purify arsenic pollution from the soil. The present study focuses on the phytoextraction of arsenic and aims to clarify the participation of microbes in the arsenic absorption by Pteris vittata. The cultivation experiments of Pteris vittata have been performed to investigate the transition of arsenic from the soil to Pteris vittata. The results show that the arsenite in the soil is promptly oxidized to arsenate and subsequently absorbed by Pteris vittata. The arsenic oxidation experiment in the soil with soil bacteria addition has been peformed, and the speciation of arsenic in the soil has been investigated. The results suggest that soil bacteria are responsible for the arsinite oxidation. T-RFLP analysis targeting to arsenite oxidase genes, aroAs, has been applied to analyze the microbial community in the rhizoshpere of Pteris vittata. The results suggest that specific aroA containing bacterial strains are involved in the arsenite oxidation prior to being absorbed by Pteris vittata.

  83. Field study on phytoremediation of the arsenic contaminated site in northeast Japan using arsenic-hyperaccumulator Pteris vittata Peer-reviewed

    Yi Huang, Mei Fang Chien, Shunsuke Otomo, Keisuke Miyauchi, Chihiro Inoue, Ginro Endo

    Proceedings of 10th International Phytortechnologies Conference 2013　299　2013/10

  84. Arsenic Contamination of Soil by the 2011 Off the Pacific Coast of Tohoku Earthquake Tsunami and Its Remediation Invited Peer-reviewed

    Mei-Fang Chien, Keisuke Miyauchi, Mei-Fang Chien, Yi Huang, Shunsuke Ohtomo, Takuya Izumi, Chihiro Inoue, Nobuyuki Kitajima, Ginro Endo

    Journals of Environmental Biotechnology　13　(1)　27-30　2013/08

    Publisher:

    ISSN： 1347-1856

  85. 宮城県主要河川沖積平野部の土壌ヒ素濃度と東北地方太平洋沖地震津波の影響 Peer-reviewed

    簡 梅芳, 宮内啓介, 井上千弘, 北島信行, 遠藤銀朗

    土木学会論文集　69　(1)　19-24　2013

    Publisher: Japan Society of Civil Engineers

    DOI： 10.2208/jscejer.69.19 　

    ISSN： 2185-6648

    More details Close

    The arsenic contamination of soil causes a huge damage on human health and production of food. In this study, the arsenic pollution of the sea shore areas of Miyagi Prefecture, Japan, where marine sediment was brought onto the land surface by the 2011 off the Pacific coast of Tohoku earthquake tsunami was investigated. Seventy eight soil samples were collected from both the tsunami disaster regions and the non-tsunami disaster regions of each major river alluvial plain in Miyagi prefecture. The arsenic contents of those soil samples were analyzed by an atomic absorption photometer. The results showed that in each river alluvial plain, the average of the arsenic content of the tsunami disaster region soil is higher than that of the non-tsunami disaster region. Furthermore, the arsenic content of each alluvial plain soil and the tsunami deposit reflected on the geological features and the anthropogenic activities (mining, hot spring, etc.) in the river basins. These results suggested that the arsenic contamination of the soil of major river alluvial plains that were suffered from the tsunami disaster was enlarged by the tsunami deposit which accumulated arsenic in the marine sediments during long time period of the river flows.

  86. Selection and application of endophytic bacterium Achromobacter xylosoxidans strain F3B for improving phytoremediation of phenolic pollutants Peer-reviewed

    Ying-Ning Ho, Dony Chacko Mathew, Shu-Chuan Hsiao, Chun-Hao Shih, Mei-Fang Chien, Hsing-Mei Chiang, Chieh-Chen Huang

    JOURNAL OF HAZARDOUS MATERIALS　219　43-49　2012/06

    DOI： 10.1016/j.jhazmat.2012.03.035 　

    ISSN： 0304-3894

  87. Mercury removal and recovery by immobilized Bacillus megaterium MB1 Peer-reviewed

    Meifang Chien, Ryo Nakahata, Tetsuya Ono, Keisuke Miyauchi, Ginro Endo

    Frontiers of Chemical Science and Engineering　6　(2)　192-197　2012/06

    DOI： 10.1007/s11705-012-1284-3 　

    ISSN： 2095-0179 2095-0187

  88. 地震・津波被災によるヒ素汚染土壌の植物による浄化技術の開発

    遠藤銀朗, 宮内啓介, 簡 梅芳, 井上千弘, 北島信行

    Chemical Engineering　57　29-34　2012

  89. Organomercurials removal by heterogeneous merB genes harboring bacterial strains Peer-reviewed

    Mei-Fang Chien, Masaru Narita, Kuo-Hsing Lin, Kazuaki Matsui, Chieh-Chen Huang, Ginro Endo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING　110　(1)　94-98　2010/07

    DOI： 10.1016/j.jbiosc.2010.01.010 　

    ISSN： 1389-1723

    eISSN： 1347-4421

  90. Mercury resistance and accumulation in Escherichia coli with cell surface expression of fish metallothionein Peer-reviewed

    Kuo-Hsing Lin, Mei-Fang Chien, Ju-Liang Hsieh, Chieh-Chen Huang

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY　87　(2)　561-569　2010/06

    DOI： 10.1007/s00253-010-2466-x 　

    ISSN： 0175-7598

  91. Distribution of mercury resistance determinants in highly mercury polluted area of Taiwan Peer-reviewed

    Mei-Fang Chien, Kao-Hung Lin, Juu-En Chang, Chieh-Chen Huang, Ginro Endo, Satoru Suzuki

    Interdisciplinary Studies on Environmental Chemistry　3　31-36　2010

  92. Bacterial mercury resistance of TnMERI1 and its’ application in bioremediation Peer-reviewed

    Chieh-Chen Huang, Mei-Fang Chien, Kuo-Hsing Lin

    Interdisciplinary Studies on Environmental Chemistry　3　23-29　2010

  93. Splicing of a Bacterial Group II Intron from Bacillus megaterium Is Independent of Intron-Encoded Protein Peer-reviewed

    Mei-Fang Chien, Saeko Tosa, Chieh-Chen Huang, Ginro Endo

    MICROBES AND ENVIRONMENTS　24　(1)　28-32　2009

    DOI： 10.1264/jsme2.ME08540 　

    ISSN： 1342-6311

  94. Expressing a bacterial mercuric ion binding protein in plant for phytoremediation of heavy metals Peer-reviewed

    Ju-Liang Hsieh, Ching-Yi Chen, Meng-Hsuen Chiu, Mei-fang Chein, Jo-Shu Chang, Ginro Endo, Chieh-Chen Huang

    JOURNAL OF HAZARDOUS MATERIALS　161　(2-3)　920-925　2009/01

    DOI： 10.1016/j.jhazmat.2008.04.079 　

    ISSN： 0304-3894

  95. Facilities for transcription and mobilization of an exon-less bacterial group II intron nested in transposon TnMERI1 Peer-reviewed

    Mei-Fang Chien, Chieh-Chen Huang, Tomonobu Kusano, Ginro Endo

    GENE　408　(1-2)　164-171　2008/01

    DOI： 10.1016/j.gene.2007.10.032 　

    ISSN： 0378-1119

  96. Development of new in situ molecular breeding method for application of microbial environment purification capability --gene introduction by bacterial intron--

    Mei-Fang Chien, Saeko Tosa, Chieh-Chen Huang, Tomonobu Kusano, Ginro Endo

    Bulletin of Environmental and Disaster Prevention Research Institute Tohoku Gakuin University　19　39-45　2007

Show all ︎Show first 5

Misc. 34

  1. ヒ素超蓄積植物根圏のマルチオミクス解析による 圃場環境におけるヒ素吸収促進要因の検討

    工藤宏史, 韓凝, 横山大稀, 簡梅芳, 菊地淳, 井上千弘

    日本農芸化学会2024年度大会要旨集　2024/03

  2. ベイジアンネットワークを用いた植物によるヒ素蓄積要因の解析

    工藤宏史, 韓凝, 横山大稀, 簡梅芳, 菊地淳, 井上千弘

    環境バイオテクノロジー学会大会プログラム講演要旨集　2022　2022

  3. Tracking arsenic dynamics in As-hyperaccumulator Pteris vittata by using Positron-Emitting Tracer Imaging System (PETIS)

    黄田毅, 黄田毅, QIAN Zhaojie, CHIEN Meifang, 宮内啓介, 宮内啓介, 遠藤銀朗, 池田隼人, 池田隼人, 菊永英寿, 渡部浩司, 鈴井伸郎, YIN Yong-Gen, 河地有木, 北島信行, 井上千弘

    アイソトープ・放射線研究発表会(Web)　58th　2021

    ISSN： 2436-4487

  4. RNA-seqによるモエジマシダのヒ素吸収・輸送に寄与する遺伝子の探索

    簡梅芳, 魏書君, 森内良太, 井上千弘

    環境バイオテクノロジー学会大会プログラム講演要旨集　2021 (CD-ROM)　2021

  5. Application of data science on phytoremediation towards identifying indicators for environmental diagnosis

    工藤宏史, HAN Ning, 横山大稀, CHIEN Mei-Fang, 菊地淳, 井上千弘

    日本生物工学会大会講演要旨集　73rd　2021

  6. 重金属高蓄積植物ハクサンハタザオ体内におけるカドミウムの吸収過程の追跡

    QIAN Zhaojie, 黄田毅, 池田隼人, 渡部浩司, 鈴井伸郎, YIN Yong-Gen, 河地有木, CHIEN Mei-Fang, 井上千弘

    アイソトープ・放射線研究発表会要旨集　56th　2019

  7. PETISによる亜鉛とカドミウムの高蓄積植物ハクサンハタザオの体内における亜鉛の挙動の追跡

    黄田毅, QIAN Zhaojie, 渡部浩司, 鈴井伸郎, YIN Yong-Gen, 河地有木, CHIEN Meifang, 井上千弘

    アイソトープ・放射線研究発表会要旨集　56th　2019

  8. Plant-induced variability in bacterial PAHs degradation in hydroponic culture of sudangrass

    DOMINGUEZ John Jewish A., CHIEN Mei-Fang, INOUE Chihiro

    環境バイオテクノロジー学会大会プログラム講演要旨集　2018　2018

  9. ハクサンハタザオにおけるカドミウムと亜鉛の輸送過程の追跡

    銭照杰, 黄田毅, 鈴井伸郎, 尹永根, 河地有木, 池田隼人, 池田隼人, 渡部浩司, 北島信行, 簡梅芳, 井上千弘

    環境バイオテクノロジー学会大会プログラム講演要旨集　2018　2018

  10. Effective removal of arsenic from soil by Pteris ferns

    23　441-443　2017/11/09

    Publisher: [地盤工学会]

  11. Dissipation of polycyclic aromatic hydrocarbons and bacterial community shift in grasses

    DOMINGUEZ John Jewish Arellano, CHIEN Mei-Fang, INOUE Chihiro

    日本微生物生態学会大会(Web)　2017　2017

  12. 亜ヒ酸酸化を指標にしたモエジマシダによるヒ素の吸収を触媒する微生物の探索

    CHIEN Meifang, 宮内啓介, HUANG Yi, 遠藤銀朗

    日本農芸化学会大会講演要旨集(Web)　2014　2A02A09 (WEB ONLY)　2014/03/05

    ISSN： 2186-7976

  13. 3P-092 Isolation and characterization of microbes in the rhizosphere of an arsenic hyper-accumulator, Pteris vittata

    Chien Mei-Fang, Lo Pei-Hsin, Makita Ryota, Miyauchi Keisuke, Endo Ginro, Inoue Chihiro

    66　217-217　2014

    Publisher: 日本生物工学会

  14. 2S-Dp05 Bioremediation of arsenic-contaminated agricultural soil using arsenic hyperaccumulator fern

    Miyauchi Keisuke, Huang Yi, Chien Mei-Fang, Endo Ginro

    66　101-101　2014

    Publisher: 日本生物工学会

  15. モエジマシダによるヒ素吸収に関与する微生物の特性解析

    簡梅芳, 小畑和貴, 宮内啓介, 遠藤銀朗

    環境バイオテクノロジー学会大会プログラム講演要旨集　2013　50　2013/05/30

  16. ヒ素高蓄積植物モエジマシダによるヒ素汚染土壌の修復

    黄毅, 簡梅芳, 宮内啓介, 北島信行, 井上千弘, 遠藤銀朗

    環境バイオテクノロジー学会大会プログラム講演要旨集　2013　30　2013/05/30

  17. モエジマシダによるヒ素高蓄積における微生物の関与

    CHIEN Meifang, 小畑和貴, 宮内啓介, 遠藤銀朗

    日本農芸化学会大会講演要旨集(Web)　2013　3C32P03 (WEB ONLY)　2013/03/05

    ISSN： 2186-7976

  18. 3P-101 Phytoremediation of soil contaminated with arsenic using arsenic-hyperaccumulator plant Pteris vittata

    Huang Yi, Chien Mei-Fang, Miyauchi Keisuke, Kitajima Nobuyuki, Inoue Chihiro, Endo Ginro

    65　213-213　2013

    Publisher: 日本生物工学会

  19. 東北地方太平洋沖地震波による土壌ヒ素汚染の実態とその修復への取り組み

    遠藤銀朗, 簡梅芳, 宮内啓介, 今野大, 本多光太郎, 井上千弘, 北島信行

    環境バイオテクノロジー学会大会プログラム講演要旨集　2012　27　2012/06/25

  20. 地震・津波被災によるヒ素汚染土壌の植物による浄化技術の開発 (特集 大震災と環境への取り組み)

    遠藤 銀朗, 宮内 啓介, 簡 梅芳

    ケミカルエンジニヤリング　57　(6)　437-442　2012/06

    Publisher: 化学工業社

    ISSN： 0387-1037

  21. 2Hp17 Study on the bacterial flora in rhizosphere of an arsenic hyper-accumulator plant and its arsenic accumulation mechanism

    Chien Mei-Fang, Miyauchi Keisuke, Endo Ginro

    64　77-77　2012

    Publisher: 日本生物工学会

  22. 固定化したBacillus megaterium MB1による水銀除去特性

    簡梅芳, 中畑涼, 小野哲矢, 宮内啓介, 遠藤銀朗

    環境バイオテクノロジー学会大会プログラム講演要旨集　44th　43　2011/06/20

  23. 2Cp18 Mercury Removal by Immobilized Sporulating Mercury Volatilizing Bacterium, Bacillus megaterium MB1

    Chien Mei-Fang, Konno Hiroshi, Konno Junpei, Nakahata Ryo, Endo Ginro

    63　131-131　2011

    Publisher: 日本生物工学会

  24. Purification and function analysis of a broad-spectrum organomercurial lyase (MerB3) from mercury resistance transposon, TnMERI1

    Mei-Fang Chien, Hui-Tzu Lin, Kuo-Hsing Lin, Ginro Endo, Chieh-Chen Huang

    JOURNAL OF BIOTECHNOLOGY　150　S54-S54　2010/11

    DOI： 10.1016/j.jbiotec.2010.08.144 　

    ISSN： 0168-1656

  25. 2P-2020 Study on distribution and diversity of mercuric reductase gene, merA in a mercury polluted site in Taiwan

    CHIEN Mei-Fang, ISHIDO Rie, TANAKI Yuko, KOYAMA Masayuki, MIYAUCHI Keisuke, CHANG Jun-En, ENDO Ginro

    22　111-111　2010

    Publisher: 日本生物工学会

  26. merA遺伝子を標的とした水銀汚染土壌における水銀耐性細菌の多様性の解析

    簡梅芳, 石堂里英, 棚木優子, 宮内啓介, 張祖恩, 遠藤銀朗

    環境バイオテクノロジー学会大会プログラム講演要旨集　40th　23　2010

  27. Mercury removal by immobilized bacteria that were genetically modified with merA and merB genes from Minamata Bay bacillus strain

    Ginro Endo, Keisuke Miyauchi, Mei-Fang Chien, Hiromi Somaki, Kyohei Yamato

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING　108　S84-S85　2009/11

    DOI： 10.1016/j.jbiosc.2009.08.248 　

    ISSN： 1389-1723

  28. 07-121 Capacity and specificity of organomercurials detoxification by heterogeneous merB genes expressed in recombinant Escherichia coli(Bioremediation) :

    Narita Masaru, Chien Meifang, Matsui Kazuaki, Huang Chiehchen, Endo Ginro

    (24)　119-119　2008

    Publisher: 日本微生物生態学会

  29. 1Dp08 Basic study on the splicing of a bacterial intron for in situ molecular breeding

    CHIEN Mei-Fang, TOSA Saeko, HUANG Chieh-chen, KUSANO Tomonobu, ENDO Ginro

    20　114-114　2008

    Publisher: 日本生物工学会

  30. 1Dp09 Study on the expression of the functional gene inserted in a trans-ferable genetic element, bacterial group II intron B.me.I1, and splicing of B.me.I1 in eukaryotic cells

    TOSA Saeko, KUMAGAI Emi, CHIEN Mei-Fang, ENDO Ginro

    20　115-115　2008

    Publisher: 日本生物工学会

  31. 原位置分子育種を目的とした細菌性グループIIイントロンのスプライシングに関する研究

    土佐彩絵子, CHIEN Meifang, 宮内啓介, 遠藤銀朗

    日本生物工学会大会講演要旨集　59th　115-115　2007/08/02

    Publisher: 日本生物工学会

  32. 1E11-2 Bacis study on the transcription and transition of a bacterial group II intron, B.me.I1

    CHIEN Meifang, TOSA Saeko, ASANO Ryo, HUANG Chiehchen, KUSANO Tomonobu, ENDO Ginro

    19　115-115　2007

    Publisher: 日本生物工学会

  33. 1H10-4 A study of transcription and mobility of a bacterial group II intron identified in Bacillus megaterium MB1

    CHIEN Meifang, HUANG Chiehchen, KUSANO Tomonobu, ENDO Ginro

    18　130-130　2006

    Publisher: 日本生物工学会

  34. 1H10-3 A study of essential factors in splicing of a bacterial group II intron identified in a mercury resistant bacteria, Bacillus megaterium MB 1

    TOSA Saeko, CHIEN Meifang, ENDO Ginro

    18　130-130　2006

    Publisher: 日本生物工学会

Show all ︎Show first 5

Presentations 51

  1. A Scope of biological alchemy by introducing advanced molecular biotechnology Invited

    Mei-Fang Chien

    International Conference on Water Environment Research for Sustainability　2024/10/29

  2. Utilization of biological functions to remove toxic metals or recover valuable metals from the aquatic environment Invited

    Mei-Fang Chien

    2024/09/18

  3. Recovery of metallic resource through advanced biotechnological approaches toward a sustainable society Invited

    Mei-Fang Chien

    International Conference on Environmental Science and Resource Management  “Environmental Protection and Resource Management Towards Sustainable Development”　2024/05/16

  4. 超蓄積植物とその根圏微生物の活用による土壌中ヒ素の生物学的除去

    簡梅芳, 楊重陽,韓凝, 井上千弘

    第28 回ヒ素シンポジウム　2023/11/30

  5. 微生物補助型ファイトエキストラクションによるヒ素含有土壌の処理 Invited

    簡梅芳, 井上千弘, 楊重陽, 韓凝

    2023年度資源・素材関係学協会合同秋季大会　2023/09/14

  6. Biorecycling of metals for a sustainable society: elucidation and utilization of biological functions Invited

    Mei-Fang Chien

    International Symposium on Advanced and Sustainable Science and Technology　2023/09/11

  7. Data science unfolds the mechanisms of environmental remediation by complex biological systems and its challenges Invited

    2023/09/04

  8. Metal biotechnologies toward environmental conservation and sustainability Invited

    Mei-Fang Chien

    International Conference on Biotechnology and Healthcare Industry　2021/12/10

  9. 複合環境下での1,4-ジオキサン生分解における非分解菌の役割を示す経験的証拠

    簡梅芳, Tusher Tanmoy Roy, 井上千弘

    日本生物工学会2021年度大会　2021/10/29

  10. 環境診断に資する指標決定に向けたファイトレメディエーションにおけるデータサイエンス

    工藤宏史, 韓凝, 横山大稀, 簡梅芳, 菊地淳, 井上千弘

    日本生物工学会2021年度大会　2021/10/29

  11. An academic journey into the interplay of plants and microorganisms toward a sustainable development of environment Invited

    Mei-Fang Chien

    2021/10/25

  12. 環境調和型バイオテクノロジーの創出を目指した生物機能と生物間作用の解明 Invited

    簡梅芳

    日本微生物学連盟理事会、日本学術会議総合微生物科学分科会・IUMS 分科会・病原体学分科会合同会議　2021/10/08

  13. 両極型微生物燃料電池の運転による硝酸性窒素除去能力の評価

    志村龍之介, 荒井雄貴, 井上千弘, 簡梅芳

    環境バイオテクノロジー学会2021年度大会　2021/09/03

  14. 農業用プラスチック資材の表面に存在する土壌微生物の評価

    佐藤花菜子, 井上千弘, 簡梅芳

    環境バイオテクノロジー学会2021年度大会　2021/09/03

  15. RNA-seq によるモエジマシダのヒ素吸収・輸送に寄与する遺伝子の探索

    簡梅芳, 魏書君, 森内良太, 井上千弘

    環境バイオテクノロジー学会2021年度大会　2021/09/02

  16. 油脂分解に寄与する細菌の単離と特徴づけ

    黄季宇, 井上千弘, 簡梅芳

    環境バイオテクノロジー学会2021年度大会　2021/09/02

  17. 微生物・植物によるヒ素汚染浄化機構の解明とその応用 Invited

    簡梅芳

    環境バイオテクノロジー学会2021年度大会　2021/09/03

  18. 機能性根圏細菌が多圃場におけるモエジマシダのヒ素ファイトエキストラクションに及ぼす影響の評価

    韓 凝, 竹岡拓人, 井上千弘, 簡 梅芳

    環境バイオテクノロジー学会2021年度大会　2021/09/02

  19. ハクサンハタザオによる圃場レベルでのCd浄化

    朴炤妍, 工藤宏史, 黄田毅, 簡梅芳, 井上千弘, 宮内啓介

    第26回地下水・土壌汚染とその防止対策に関する研究集会　2021/06

  20. Characterization of novel 1,4-dioxan-degrading bacteria isolated from a stable 1,4-dioxane-degrading microbial consortium

    Tanmoy Roy Tusher, Takuya Shimizu, Mei-Fang Chien, Chihiro Inoue

    日本農芸化学会2021年度大会　2021/03

  21. The interactions between Pteris vittata and its rhizosphere contribute to As phytoextraction

    Chongyang Yang, Ning Han, Ying-Ning Ho, Yu-Liang Yang, Hideaki Nojiri, Mei-Fang Chien, Chihiro Inoue

    日本農芸化学会2021年度大会　2021/03

  22. 根圏メタボロミクス解析を起用したヒ素のファイトエキストラクション過程における代謝産物および代謝経路の調査

    韓 凝・楊 重陽・ホ インニン・楊玉良・簡 梅芳・井上 千弘

    日本農芸化学会2021年度大会　2021/03

  23. ポットを用いたモエジマシダのヒ素吸収における土壌との相互作用の検討

    韓 凝, 楊 重陽, ホ, インニン・簡 梅芳, 井上 千弘

    日本農芸化学会2020年度大会　2020/03

  24. Molybdate recovery using immobilized bioengineered Saccaromyces cerevisiae

    Audrey Stephanie, Mei-Fang Chien, Naoya Ikeda, Chirihi Inoue

    The international biohydrometallrugy symposium 2019　2019/10

  25. Investigation of CCl4 degrading consortia via quantitative next generation sequencing

    Stari Lazo Leonardo Alfredo, Mei-Fang Chien, Chihiro Inoue

    2019/06/15

  26. Pseudomonas sp. strain m318 increased As(III)--oxidase gene in the rhizosphere of As hyperaccumulators and elevated the As phytoextraction

    Chongyang Yang, Mei-Fang Chien, Chihiro Inoue

    2019/06/15

  27. Bioengineering of a stable molybdate binding system using Saccharomyces cerevisiae

    Stephanie Audrey, Mei-Fang Chien, Naoya Ikeda, Kengo Kubota, Chihiro Inoue

    2019/03/24

  28. シダ類植物による土壌中ヒ素の効果的除去

    簡梅芳, 牧田涼太, 井上千弘

    地下水・土壌汚染とその防止対策に関する研究集会　2017/11/09

  29. Influence of Temperature on Arsenic Uptake by Pteris cretica from Hydroponic Solution International-presentation

    Farzana Rahman, Kazuki Sugawara, Yi Huang, Mei-Fang Chien, Chihiro Inoue

    the 14th International Phytotechnologies Conference　2017/09/25

  30. Comparative analysis of arsenate reductase gene expression in two arsenic hyperaccumulator Pteris ferns International-presentation

    Shujun Wei, Mei-Fang Chien, Chihiro Inoue

    the 14th International Phytotechnologies Conference　2017/09/25

  31. WBC11 as a Potential Gene for Cadmium Accumulation/Transportation in Arabidopsis halleri ssp. gemmifera International-presentation

    C. D. A. Wiyono, M.-F. Chien, C. Inoue

    the 14th International Phytotechnologies Conference　2017/09/25

  32. Adsorption of Cd and Zn to the root of Arabidopsis halleri ssp. gemmifera International-presentation

    Z.-J. Qian, Y. Huang, M.-F. Chien, C. Inoue

    the 14th International Phytotechnologies Conference　2017/09/25

  33. VISUALIZATION OF ZINC TRANSLOCATION DYNAMIC IN HYPERACCUMULATOR ARABIDOPSIS HALLERI SSP. GEMMIFERA AND NON-ACCUMULATOR ARABIDOPSIS THALIANA International-presentation

    Y. Huang, Z. Qian, M.-F. Chien, K. Sugawara, N. Suzui, Y. Yin, N. Kawachi, H. Watabe, N. Kitajima, C. Inoue

    the 14th International Phytotechnologies Conference　2017/09/25

  34. Construction of a cell surface engineered yeast aims to selectively recover molybdenum, a rare metal International-presentation

    Mei-Fang Chien, Naoya Ikeda, Kengo Kubota, Chihiro Inoue

    22. International Biohydrometallurgy Symposium　2017/09/24

  35. 金属を操る微生物とそれらを利用した環境技術の開発

    分析化学会東北支部会若手の会　2017/07/14

  36. レアメタルの持続可能な利用を目的とした酵母によるモリブデンの選択的回収・濃縮システムの構築

    池田 直也, 簡 梅芳, 久保田 健吾, 井上 千弘

    日本農芸化学会2017年度大会　2017/03/17

  37. Analysis of arsenate reductase gene expression in Pteris vittata

    Shujun Wei, Mei-Fang Chien, Kazuki Sugawara, Chihiro Inoue

    日本農芸化学会2017年度大会　2017/03/17

  38. Study on arsenic remove from the soil by hyperaccumulators and their rhizobacteria forward to arsenic phytoremediation International-presentation

    Mei-Fang Chien, Ryota Makita, Kazuki Sugawara, Chihiro Inoue

    2016 Conference of Soil and Groundwater Environment　2016/10/13

  39. Phenotypic and genotypic analysis of arsenic accumulation and transformation in three Pteris ferns International-presentation

    Shujun Wei, Mei-Fang Chien, Kazuki Sugawara, Chihiro Inoue

    13th International Phytotechnologies Conference　2016/09/26

  40. Analysis of bacteria in rhizosphere of two arsenic hyperaccumulators and their effects on phytoremediation of arsenic International-presentation

    Ryota Makita, Mei-Fang Chien, Hirofumi Nagayama, Chihiro, Inoue

    13th International Phytotechnologies Conference　2016/09/26

  41. Phytofiltration potential of Pteris multifida for accumulating As, Pb, Cd and Se from mixed metal solution International-presentation

    Farzana Rahman, Kazuki Sugawara, Mei-Fang Chien, Chihiro, Inoue

    13th International Phytotechnologies Conference　2016/09/26

  42. The arsenic accumulation and arsenate reductase gene of three Pteris ferns

    Shujun Wie, Han Wang, Kazuki Sugawara, Meifang Chien, Chihiro Inoue

    日本農芸化学会2016年度大会　2016/03/27

  43. Study on As uptake and rhizobacteria of two As hyperaccumulators forward to As phytoremediation International-presentation

    Ryota Makita, Kazuki Sugawara, Chihiro Inoue

    International Biohydrometallurgy Symposium 2015　2015/10/05

  44. Characterization of rhizobacteria isolated from arsenic hyperaccumulator ferns International-presentation

    Ryota Makita, Hirofumi Nagayama, Kazuki Sugawara, Chihiro Inoue

    12th International Phytotechnologies Conference　2015/09/27

  45. ヒ素超蓄積植物の栽培実験およびその根圏微生物相の解析

    牧田涼太, 永山浩史, 井上千弘, 簡梅芳

    資源素材学会　2015/09/08

  46. 植物のヒ素吸収とヒ素耐性に寄与する根圏微生物の機能解析

    簡梅芳, 牧田涼太, 永山浩史, 井上千弘

    日本環境バイオテクノロジー学会　2015/06/29

  47. ヒ素超蓄積シダ植物モエジマシダとイノモトソウの根圏微生物の特徴づけ

    簡梅芳, 牧田涼太, 菅原一輝, 井上千弘

    日本水環境学会2014年度大会　2015/03/16

  48. Analysis of 1,2-dichlorobenzene degrading consortium International-presentation

    Ge Cui, Koichi Suto, Mei-Fang Chien, Chihiro Inoue

    International Symposium on Evolutionary Genomics and Bioinformatics 2014　2014/11/07

  49. Study on As uptake and removal by As hyper-accumulator Pteris vittata and it’s rhizosphere bacteria International-presentation

    Mei-Fang Chien, Kazuki Obata, Yi Huang, Keisuke Miyauchi, Ginro Endo, Chihiro Inoue

    11th International Phytotechnologies Conference　2014/09/30

  50. ヒ素超蓄積植物モエジマシダの根圏微生物の単離および特徴づけ

    簡 梅芳, 羅 佩昕, 牧田 涼太, 宮内 啓介, 遠藤 銀朗, 井上 千弘

    第６６回日本生物工学会大会（2014)　2014/09/09

  51. 微生物・植物による環境調和型 バイオテクノロジーの創出と課題 Invited

    簡梅芳

    理研-東北大科学技術ハブ共同研究プログラム採択課題によるシンポジウム　2021/12/14

Show all Show first 5

Research Projects 21

  1. 環境・生物間作用の解明と活用による現場適応型ヒ素除去バイオモデルの確立

    簡 梅芳, 菊地 淳, 井上 千弘

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2024/04 - 2028/03

  2. Innovative Resource Recycling Use of Woody Biomass Ash and Nutrient Dynamics Analysis: New Developments for Sustainable Agriculture

    Offer Organization: Bio-oriented Technology Research Advancement Institution (BRAIN)

    2024/09 - 2027/03

  3. 生物利用による製錬過程に伴う環境負荷とコストの低減に関する研究

    簡梅芳, 上高原理暢

    Offer Organization: DOWAホールディングス

    System: DOWAテクノファンド

    2023/06 - 2025/05

  4. 生物学的金属イオン回収のための外部刺激応答性担体の創製

    上高原 理暢, 梅津 将喜, 簡 梅芳

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2022/06 - 2025/03

    More details Close

    微生物の付着・脱着の制御が可能な担体の創製に向けて、紫外線や超音波などによる外部刺激に応答する担体の創製に取り組んだ。純チタン金属を種々の条件で酸化処理することによりその表面に酸化チタン層を作製し、その表面上での微生物の挙動について調べ、陽極酸化を施すと酸化処理していない試料および大気酸化した試料よりも酵母が付着しやすいことを明らかにしている。今年度は、陽極酸化したチタン金属表面に酵母を付着させた後に紫外線を照射することで表面にラジカルを発生させて、酵母の付着に寄与する有機物を分解することで酵母の脱着を試みた。その結果、酵母の代謝活性が見られなくなったが、酵母はあまり脱着していない様子が観察された。有機分解能力が十分でなく脱着が起きなかった可能性を考え、陽極酸化したチタン金属表面の光触媒活性を高めることを考えた。陽極酸化したチタン金属を熱処理することで、紫外線を照射した際の有機物分解能を向上させることができた。しかし、その熱処理した陽極酸化チタン金属表面でも紫外線により酵母の脱着はあまり見られなかった。そこで、外部刺激として脱離に寄与する振動とラジカル生成を期待できる超音波照射を検討した。超音波照射すると、熱処理した陽極酸化チタン金属表面からの酵母の脱着が著しく促進されることが分かった。すなわち、超音波照射は酵母を脱着させるための有効な手段となることが分かった。今後、陽極酸化チタン金属表面での特異的な現象であるかどうかも検証していく予定である。一方で、酵母の付着と脱着には、酵母を付着させる際に用いる溶液の影響も大きいことが分かった。接着と脱着のメカニズム解明に重要な知見であると考えるため、溶液の影響についても今後検証していく予定である。

  5. 遺伝子改変酵母を利用した金属回収技術の能力検証

    井上千弘

    Offer Organization: 本田技研工業

    2023/06 - 2024/03

  6. Realization of a novel metallic resource recovery technology by introducing the advanced molecule biotechnology method

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    Institution: Tohoku University

    2019/10 - 2024/03

  7. 循環型社会の実現に向けた生物学的金属応答機能の活用

    Offer Organization: 株式会社 資生堂

    System: 資生堂 女性研究者サイエンスグラント第 15 回

    2022/06 - 2023/05

  8. 持続可能な環境修復を実現するための環境・生物要因解析―環境科学プラットフォーム拠点 の形成に向けてー

    横山大稀, 菊地淳, 井上千弘, 工藤宏史, 韓凝, 松本朋子

    Offer Organization: 理化学研究所、東北大学

    System: 令和4年度 理研-東北大 科学技術ハブ 共同研究プログラム

    Institution: 理化学研究所、東北大学

    2022/04 - 2023/03

  9. Elucidation and application of hyperaccumulating functions of plants towards remediation of heavy metals contamination

    Inoue Chihiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2019/04 - 2023/03

    More details Close

    Following results were obtained with regard to the arsenic-hyperaccumulating plant Pteris vittata and the cadmium-hyperaccumulating plant Arabidopsis halleri ssp. gemmifera. Gene expression analysis of each plant organ under the stress of toxic elements (arsenic or cadmium), two-dimensional mapping and chemical morphology analysis of each toxic elements absorbed by the plants, and dynamics of both elements in the hyperaccumulating plants using Positron Emitting Tracer Imaging System are deepened our understanding of the mechanism of uptake and accumulation of toxic elements in these hyperaccumulator. In addition, network analysis of the field trial data of phytoremediation using these hyperaccumulating plants extracted the factors that improve the remediation efficiency.

  10. Preparation of photoresponsive scaffolds that enable highly efficient biological recovery of rare metals

    Kamitakahara Masanobu

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2019/06/28 - 2022/03/31

    More details Close

    We prepared the titanium plates with titanium oxide layer on their surface by various oxidation treatments for the creation of the scaffolds that can control the attachment and detachment of microorganisms. By anodizing the titanium plate, it was possible to form a highly hydrophilic and rough titanium oxide layer. This anodized sample had higher adhesion to yeast than the unoxidized sample. When the yeast attached to the surface-oxidized titanium plate was irradiated with UV, the metabolic activity of the yeast decreased and the photocatalytic activity of titanium oxide was expressed. On the other hand, it was found that the yeast expressing the molybdenum-binding protein loaded on the porous ceramic could also recover molybdenum.

  11. 持続可能な環境修復を実現するための環境・生物要因解析―環境科学プラットフォーム拠点 の形成に向けてー

    横山大稀, 菊地淳, 井上千弘, 工藤宏史, 韓凝, 村山昂平

    Offer Organization: 理化学研究所、東北大学

    System: 令和3年度 理研-東北大 科学技術ハブ 共同研究プログラム

    Institution: 理化学研究所、東北大学

    2021/04 - 2022/03

  12. レアメタル回収バイオリアクターのための光応答性担体の創製 Competitive

    上高原理暢, 簡梅芳

    Offer Organization: 日本学術振興会

    System: 科学研究費挑戦的研究（萌芽）

    2019/06 - 2022/03

  13. Development of fundamental technologies for rare metal recovery by exploring metal responsible bioresource

    Chien Mei-Fang

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2019/04 - 2022/03

    More details Close

    This study aimed to discover the metal-responsible bioresources, to construct novel metal-recovery microorganisms, and to develop a biosystem by applying metal-recovery microorganisms. Regarding the search for bioresources, we succeeded in obtaining binding protein genes of two rare metals, molybdenum and nickel, in finding microorganisms that have detoxification genes for the toxic metals arsenic and mercury in a special structure, and in analyzing the expression of genes that contribute to the uptake of arsenic, cadmium, and zinc from hyperaccumulator plants. Regarding the construction of metal-recovery microorganisms, we succeeded in producing molybdenum-adsorbing yeast and nickel- and cobalt-adsorbing Escherichia coli cells, and were also able to study the fixation carriers for these microorganisms. These results have published six papers in academic journals and given seven presentations in academic conferences.

  14. 排水からレアメタルを回収するための生物学的濃縮・回収技術の確立 Competitive

    簡梅芳

    Offer Organization: 公益財団法人 岩谷直治記念財団

    System: 岩谷科学技術研究助成

    2019/04 - 2020/03

  15. Development of a biological recovery and concentration technology aimed at sustainable use of rare metals

    Chien Mei-Fang

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2018/06 - 2020/03

    More details Close

    The study aimed to develop biological recovery techniques to rare metal toward a sustainable using of rare metals. The achievements of this study are as follows: 1, Organic mercury degrading enzyme MerB3 in a mercury resistant bacterium MB1 showed the potential to be a biological antidote. 2, mercury resistant bacterium MB24 contains both mercury and arsenic resistance operons as a special structure. The above results were published in English journals Journal of Environmental Biotechnology and Microorganisms, respectively. 3, the molybdenum adsorbing yeast was constructed by expressing molybdenum binding protein on the cell surface of yeast cells. Besides, the efficiency of adsorption got increasing through immobilizing the yeast cells. The above results have been presented at Japan Society for Bioscience, Biotechnology and Agrochemistry and International Biometallurgy Symposium 2019.

  16. 植物・微生物による効果的なヒ素除去機構の解明と汚染現場に適用するための技術提案 Competitive

    簡梅芳

    Offer Organization: NPO環境エネルギー技術研究所

    System: 環境エネルギー技術研究所第９回研究助成

    2018/04 - 2019/03

  17. レアメタルの濃縮回収を同時にこなす革新的な生物学的地下資源開発技術の基盤構築 Competitive

    簡梅芳

    Offer Organization: 公益財団法人 新井科学技術振興財団

    System: 平成２７年度研究助成

    2016/04 - 2018/09

  18. 植物根圏環境の解明による新しいファイトエクストラクション技術の構築と展開 Competitive

    簡梅芳, 久保田健吾, 大坪和香子

    Offer Organization: 東北大学 学際科学フロンティア研究所

    System: 領域創成研究

    2015/04 - 2017/03

  19. Development o phytoremediation to efficiently remove arsenic from polluted soil environment

    Chien Mei-Fang

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    2013/04 - 2016/03

    More details Close

    This study aimed to develop an efficient phytoremediation technology to remove arsenic from polluted soil using arsenic hyperaccumulator ferns. The results obtained from this study were as bellow: 1, the soil bacteria played an important role in arsenic oxidation which was necessary before being absorbed by the ferns; 2, the rhizobacterial community of arsenic hyper accumulator ferns was composed of soil bacteria with adapting to the ferns; 3, the rhizobacteria isolates that are capable of arsenite oxidation and plant growth promotion were obtained; 4, the results of co-cultivation experiment using above isolated strains and hyper accumulator ferns showed that both biomass and accumulated arsenic of inoculated ferns increased much more than that of non-inoculated ones did. These results provided hints when linking microbial arsenic transformation to arsenic uptake by ferns which is important when designing pre-treatment procedures of phytoremediation.

  20. 水銀汚染水の生物学的除去・回収するバイオリアクターの開発 Competitive

    簡梅芳

    Offer Organization: 公益財団法人 岩谷直治記念財団

    System: 岩谷科学技術研究助成

    2011/04 - 2012/03

  21. Development of in situ bioremediation technology to prevent repetition of Minamata disease in the world

    ENDO Ginrou, MIYAUCHI Keisuke, KAN Baihou

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku Gakuin University

    2009 - 2011

    More details Close

    In this study, we analyzed merA genes from the soil sample of a mercury contaminated site. The result showed that there is big diversity between merA genes. Second study was conducted to wide range of merA gene analysis. The results showed that there are many new merA genes in the mercury contaminated soil sample. In the final stage of this research, direct interspecies gene transfer was investigated by using filter-mating method and sandwich-meting method. The mercury resistant and tetracycline resistant bacterial colonies were observed on the culture plates. However, those resistant bacteria were not stable in the liquid culture. From these results, it was concluded that interspecies gene transfer of mercury resistance gene needs special vectors for the horizontal gene transfer.

Show all Show first 5

Teaching Experience 9

  1. 環境修復生態学　東北大学大学院環境科学研究科

  2. エネルギー環境入門（オムニバス）　東北大学工学部機械知能・航空工学科

  3. 生態学合同講義（オムニバス）　東北大学大学院環境科学研究科

  4. 都市水環境論（オムニバス、英語開講）　東北大学大学院環境科学研究科

  5. 環境システム学II　東北大学工学部機械知能・航空工学科

  6. 環境システム学I　東北大学工学部機械知能・航空工学科

  7. Energy, resources and sustainability　Tohoku University

  8. 環境科学概論（オムニバス、日本語・英語開講）　東北大学大学院環境科学研究科

  9. 環境科学演習（英語開講）　東北大学大学院環境科学研究科

Show all Show first 5

Social Activities 2

  1. 東北大学女性限定研究者の採用・昇進枠創設へ

    2017/08/17 -

    More details Close

    東北大学が平成２９年度から5年間女性限定の研究者採用・昇進枠を創設することに当たって、現場で働く女性研究者の現状として取材を受けました。

  2. オープンキャンパス for 女子高校生 2015

    東北大学オープンキャンパスfor女子高校生

    2015/07/29 -

Other 3

  1. 「環境研究とデータサイエンスの接点とそれから見えるもの」シンポジウム開催

    More details Close

    自然生態系における事象は、気象・地質・地形・海流等の環境的要因と生物的要因が複雑に作用しあうことにより生み出されます。 このような複雑系の理解・予測、そして制御が、21世紀の環境科学において最も挑戦的な課題の一つであり、 その達成には長期間にわたる環境モニタリングデータの蓄積とデータの解析が不可欠です。 本シンポジウムでは、この課題の達成に挑んでいる環境科学及びデータサイエンスの研究者による研究成果を共有し、 参加者の皆さまと共に、環境科学とデータサイエンスの接点とそれから見えるものについて考えます。 (本シンポジウムは、東北大学大学院環境科学研究科第１３１回コロキウム環境および令和３年度理研-東北大科学技術ハブ共同研究プログラム採択課題によるシンポジウムとして開催しました） 東北大学大学院環境科学研究科のHPからも確認できます。 http://www.kankyo.tohoku.ac.jp/news/event/e20211214.html

  2. レアメタルの濃縮回収を同時にこなす革新的な生物学的地下資源開発技術の基盤構築

    More details Close

    日本のレアメタルはほぼ海外からの輸入に頼っているが、供給が困難時に備えて、最も欠かせない７種類のレアメタルは国家に備蓄されている。合金や機械産業に多く利用されるモリブデンはその一つであり、２１日分も常に備えている。一方、モリブデンは全ての生物にとって必須な微量元素である。生物は環境中のモリブデンを感知し、結合して輸送したり取り込んだりするシステムが備えられている。本研究は、レアメタルのモリブデンを最初のターゲットとして、環境におけるモリブデン応答遺伝子を探索した上、生物工学を活かした効率的濃縮・回収システムの創出を目指す。本研究は以下三つの研究項目にわけて実施する：（１）モリブデン応答遺伝子の探索、（２）レアメタルの濃縮システムの基盤技術の確立、（３）濃縮したレアメタルの回収システムの基盤技術の確立。

  3. 水銀汚染水の生物学的除去・回収するバイオリアクターの開発

    More details Close

    水銀及び水銀化合物は様々な産業において使われており、日本では年間約14トン、世界的には年間約4000トンの需要量がある。しかし、高い生物毒性を持つ水銀化合物は、環境中の残留や生態系での生物濃縮などの作用を通して、世界的に大変深刻な健康被害及び環境公害問題を引き起こしており、日本においては、「水俣病」の原因環境汚染物質と確認された。水俣病のような被害が世界において二度と起こらないように、既存の水銀汚染に対する効果的、経済的、かつ安全な除去・回収技術の開発と適用が必要とされる。環境中には、水俣病の原因物質である有機水銀等の水銀化合物を分解し毒性の低い金属水銀に変換する微生物が存在しており、これらの微生物の持つ水銀浄化能力を利用した効果的な環境浄化（バイオレメディエーション）技術は、深刻化する水銀汚染を改善するための有効な対策の一つと考えられる。申請者はこれまで環境中の水銀還元細菌を持つ水銀耐性遺伝子の分子生物学的特性、環境中の生態学的分布、またバイオレメディエーションの開発に向けた組み換え微生物や植物の研究を行ってきた。本研究は、水銀還元環境細菌を固定化することにより、安全かつ有効な水銀汚染処理に適用できる生物学的水銀除去・回収システムの開発を目的とする。