Details of the Researcher

PHOTO

Takeyoshi Koseki
Section
Graduate School of Dentistry
Job title
Professor
Degree

  • 博士（歯学）（東京医科歯科大学）

Research History 5

  • 2001/04 - 2002/11
    九州歯科大学　助手

  • 1996/04 - 2001/03
    国立感染症研究所　主任研究員

  • 1997/03 - 1999/03
    ミシガン大学医学部

  • 1995/04 - 1996/03
    科学技術庁　特別研究員

  • 1994/11 - 1995/03
    ヒューマンサイエンス財団　流動研究員

Education 3

  • Tokyo Medical and Dental University　Graduate School, Division of Dental Research　歯周治療学

    - 1993/03/31

  • Tohoku University　Faculty of Dentistry

    - 1988/03

  • Tohoku University　Faculty of Dentistry

    - 1982/03/31

Committee Memberships 12

  • 日本歯科医療管理学会　評議員

    2006/09 - Present

  • 日本歯科医療管理学会　評議員

    2006/09 - Present

  • 東北大歯学会　評議員

    2006/04 - Present

  • 東北大歯学会　評議員

    2006/04 - Present

  • 日本口腔衛生学会　編集委員

    2005/05 - Present

  • 日本口腔衛生学会　編集委員

    2005/05 - Present

  • 日本口腔衛生学会　理事

    2003/09 - Present

  • 日本口腔衛生学会　理事

    2003/09 - Present

  • 東北大学歯学会　理事

    2003/04 - 2006/04

  • 東北大学歯学会　理事

    2003/04 - 2006/04

  • 東北大歯学会　理事

    2003/04 - 2006/03

  • 東北大歯学会　理事

    2003/04 - 2006/03

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Professional Memberships 6

  • 東北大歯学会(2006/04- 評議員)

  • 東北大歯学会(2003/04-2006/03 理事)

  • 日本歯科医療管理学会(2006/09- 評議員)

  • 東北大学歯学会(2003/04-2006/04 理事)

  • 日本口腔衛生学会(2005/05- 編集委員)

  • 日本口腔衛生学会(2003/09- 理事)

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Research Interests 3

  • 予防

  • 歯周病

  • 齲蝕

Research Areas 1

  • Life sciences / Conservative dentistry and endodontics /

Papers 132

  1. Elucidation of Factors Affecting Anterior Occlusion in Primary Dentition Based on the Japan Environment and Children's Study. International-journal

    Risa Ishiko, Kotaro Sena, Ichie Koseki, Masumi Sasai, Chiharu Ota, Takeyoshi Koseki

    Children (Basel, Switzerland)　12　(2)　2025/02/19

    DOI： 10.3390/children12020254 　

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    Background/Objectives: Malocclusion in primary dentition affects permanent dentition. However, the factors contributing to malocclusion in the oral cavities of children have not been fully elucidated. We hypothesized that environmental factors affect malocclusion in the primary dentition of the Japanese population and aimed to identify factors associated with anterior occlusion in primary dentition. Methods: The study involved 3793 parent-child pairs from the Miyagi Regional Centre as part of a supplementary survey to the Japan Environment and Children's Study, a cohort study. A questionnaire assessing oral development and environmental factors was administered to parent-child pairs who consented to participate. Parents assessed anterior occlusion when their children were 3.5 years old. Results: The maxillary primary central incisors tended to erupt earlier in the open bite group. Significantly more children in this group were breastfed until 1 year and drank ionic beverages at 1.5 years. In addition, thumb sucking or pacifier use was significantly common at 2 years of age. A correlation was observed between the mother's body mass index (BMI) before and after pregnancy and anterior occlusion. Conclusions: In the open bite group, the occlusion status of the anterior teeth at 3.5 years of age showed distinctive results influenced by the eruption period of the primary anterior teeth, oral habits, intake of sweetened beverages, and maternal BMI. These findings suggest that parental observation could be useful for screening children for malocclusion until the eruption of permanent dentition.

  2. Timing of Primary Tooth Eruption in Infants Observed by Their Parents. International-journal

    Mina Dodo, Chiharu Ota, Motohiro Ishikawa, Ichie Koseki, Junichi Sugawara, Nozomi Tatsuta, Takahiro Arima, Nobuo Yaegashi, Takeyoshi Koseki

    Children (Basel, Switzerland)　10　(11)　2023/10/25

    DOI： 10.3390/children10111730 　

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    BACKGROUND: The timing of primary teeth eruption is a visible indicator of infant physical growth other than body weight or height. It also reflects neurological integrity and development as well as nutrition, socioeconomic state, or underlying diseases. Therefore, the timing of primary teeth eruption is one of the major concerns for parents in health checkups for infants and children. However, the detailed developmental timing of teeth eruption differs depending on the survey methodology, country, or generation. We hypothesized that the timing of primary teeth eruption differs between the medical checkup by dentists and the daily records by parents. METHODS: We conducted a questionnaire survey on the date of eruption of primary teeth as an adjunct study among Miyagi Regional Center participants in the Japan Environment and Children's Study (JECS), a large-scale birth cohort study. A total of 1695 responses (3793 participants) were analyzed. RESULTS: The median ages of eruption were 7.1 months (male) and 7.6 months (female) for mandibular primary central incisors, 8.7 months (male) and 9.2 months (female) for maxillary primary central incisors, 10.0 months (male) and 10.3 months (female) for maxillary primary lateral incisors, and 10.4 months (male) and 10.8 months (female) for mandibular primary lateral incisors, which were earlier than the reported timings based on dental check-ups. Comparing the eruption time of preterm and term infants, the eruption time was earlier in preterm infants in the corrected ages. CONCLUSIONS: The eruption timing observed and described by the parents is earlier than that examined by dentists at regular check-ups. In addition to examining the primary teeth eruption of full-term birth children, we also examined that of preterm birth children because of the increasing number of premature births. To the best of our knowledge, this is the first report from a large cohort study to clarify the eruption time of primary teeth monitored by parents.

  3. 新型コロナウイルス感染症の流行ががん口腔支持療法受診に及ぼす影響

    瀬名 浩太郎, 石河 理紗, 丹田 奈緒子, 百々 美奈, 加藤 翼, 小関 健由

    口腔衛生学会雑誌　73　(増刊)　204-204　2023/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  4. 周術期等口腔管理に対する患者意識や歯科受診における質問紙調査

    百々 美奈, 石河 理紗, 加藤 翼, 瀬名 浩太郎, 丹田 奈緒子, 小関 健由

    みちのく歯学会雑誌　53　(1-2)　84-84　2022/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  5. Influence of alcohol sensitivity on bone metastases and skeletal-related events in primary operable breast cancer: A retrospective cohort study

    Naoko Tanda, Hiroshi Tada, Jumpei Washio, Nobuhiro Takahashi, Takanori Ishida, Takeyoshi Koseki

    PLOS ONE　17　(6)　e0269335-e0269335　2022/06/03

    Publisher: Public Library of Science (PLoS)

    DOI： 10.1371/journal.pone.0269335 　

    eISSN： 1932-6203

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    Background Bone metastases in breast cancer patients are a common concern for medical doctors and dentists. Bone-modifying agents, which are necessary to prevent skeletal-related events (SREs), are associated with osteonecrosis of the jaw as an adverse side effect. Hypersensitivity to alcohol is an unfavorable response caused by deficiency of aldehyde dehydrogenase-2 (ALDH2) activity. Inactive ALDH2 is associated with osteoporosis, but its influence on bone metastases is unclear. The aim of our study was to evaluate the effects of alcohol sensitivity on bone metastases and SREs in primary operable breast cancer patients. Methods We retrospectively analyzed patients who were administered docetaxel, an anti-tumor agent, for histologically diagnosed breast cancer between April 2004 and September 2015. Alcohol sensitivity was assessed based on medical records of hypersensitivity to alcohol. The primary endpoint was time to bone metastases and the secondary endpoint was time to first SRE from the initial docetaxel administration. Data were stratified by alcohol sensitivity and tumor stages, and differences were estimated by the Kaplan-Meier method. Prognostic risk factors were analyzed by the multivariate Cox proportional hazards model. Results The median follow-up period of patients with high sensitivity to alcohol (n = 45) was 54 months and that for those with low sensitivity (n = 287) was 64 months. Stratification by alcohol sensitivity revealed that tumor stage exhibited significant correlations with the cumulative incidence of bone metastases in low-sensitivity patients; however, no differences were found in high-sensitivity patients. In multivariate analysis, alcohol sensitivity was a significant prognostic risk factor for bone metastases (HR 2.721, 95% CI 1.268–5.841, P = 0.010). Conclusion Alcohol sensitivity may be a prognostic risk factor for bone metastases. More detailed genetic investigations and metabolic analyses are needed.

  6. 医科から紹介された周術期口腔管理患者の口腔に対する意識について

    百々 美奈, 石河 理紗, 加藤 翼, 丹田 奈緒子, 小関 健由

    口腔衛生学会雑誌　72　(増刊)　101-101　2022/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  7. Impact of Dental Referral Prior to Elective Surgery on Postoperative Outcomes. International-journal

    Hanako Suenaga, Jun Aida, Akira Kumasaka, Aya Shibamoto, Satoko Sato, Yuta Shinohara, Takenori Hatakeyama, Hirofumi Yamaguchi, Keiichi Sasaki, Tetsu Takahashi, Takeyoshi Koseki, Chris Peck, Masahiro Iikubo

    Journal of the American Medical Directors Association　23　(4)　638-645　2022/04

    DOI： 10.1016/j.jamda.2022.01.071 　

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    OBJECTIVES: Oral bacteria may contribute to postoperative infectious complications including postoperative pneumonia or surgical site infection. The aim of this study was to investigate the impact of preoperative dental care on postoperative outcomes among surgical patients under general anesthesia. DESIGN: Retrospective cohort study. SETTING AND PARTICIPANTS: We analyzed clinical records of major surgical patients at a university hospital between 2016 and 2018. Subjects were categorized into either the preoperative dental care group, those being referred to dentists by their surgeons based on an individual surgeon's judgment for dental care before surgery, or the control group. METHODS: The primary outcome was postoperative infectious complications. Secondary outcomes were postoperative inflammation markers (C-reactive protein and fever), and economic outcomes (postoperative length of hospital stay and medical expenses). As the main analysis, the average treatment effects of the preoperative dental care were obtained from the augmented inverse-probability weighting (AIPW) method with consideration of demographics and perioperative risk factors to estimate causal effect of the intervention from the observational data. Then, stratified analyses by age and surgical sites were conducted with the inverse-probability weighting and linear regression methods, respectively. RESULTS: In the AIPW estimation, compared with the control group, the care group saw a significantly lower rate of postoperative infection (average treatment effect -3.02) and shorter fever duration (-2.79 days). The stratified analysis by age revealed significant positive impact of dental care in all age groups, including the highest treatment effects observed among patients younger than 60. Also, treatment effect was observed in wider surgical sites than previously known. CONCLUSION/IMPLICATIONS: This study indicates a significant impact of preoperative dental care on preventing postoperative infection and inflammation. Along with old age or certain types of surgeries in which advantages of dental referral have been already known, preoperative dental referral could be beneficial for broader types of patients.

  8. Dental care using an oral appliance to support hematopoietic stem cell transplantation for NK/T cell lymphoma, nasal type, with palatal perforation.

    Hanako Suenaga, Masahiro Iikubo, Toru Tamahara, Mina Dodo, Chris Peck, Takeyoshi Koseki, Hideo Harigae, Keiichi Sasaki

    Journal of prosthodontic research　66　(2)　353-356　2021/07/15

    DOI： 10.2186/jpr.JPR_D_20_00270 　

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    PATIENT: A 33-year-old man diagnosed with extranodal natural killer cell/T-cell lymphoma, nasal type (ENKTCL-NT) inducing palatal perforation was referred to the perioperative oral care support center of Tohoku University Hospital for dental care to support cancer treatment including chemotherapy and hematopoietic stem cell transplantation (HSCT). Dental review during chemotherapy revealed mucositis suspected to be caused by mucosal trauma from altered jaw function (chewing and speech) due to palatal perforation. Although the patient was already in the cleanroom, an oral appliance as well as conservative care as recommended in oral management guidelines for HSCT were used to prevent worsening of oral mucositis at subsequent HSCT including High-dose chemotherapy and total body irradiation. After HSCT, a prosthodontist fitted a palatal obturator made by a dental technician and an oral surgeon reviewed the necrotic bone and removed the sequestra according to the changes in the palate. This approach involving a multidisciplinary team including a hematologist improved the impaired oral function and minimized oral complications . DISCUSSION: ENKTCL-NT and its treatment have a significant impact on patients' oral status. Hence, it is important to provide customized dental care based on previously endorsed guidelines according to the type of disease, treatment requirements, and oral and systemic status. CONCLUSIONS: This report indicated the importance of dental care with a customized plan before, during, and after HSCT for ENKTCL-NT with multidisciplinary supportive care for cancer patients to improve the impaired oral function and to minimize oral complications.

  9. HbA1cと歯周ポケットの状態および喪失歯数の疫学的関係

    渡辺 俊吾, 岩永 賢二郎, 百々 美奈, 石河 理紗, 飯嶋 若菜, 加藤 翼, 丹田 奈緒子, 相田 潤, 小関 健由

    口腔衛生学会雑誌　70　(3)　129-135　2020/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  10. 東北大学病院周術期口腔支援センター・予防歯科における紹介患者の動向

    百々 美奈, 岩永 賢二郎, 玉原 亨, 飯嶋 若菜, 石河 理紗, 猪狩 真奈, 渡辺 俊吾, 田中 篤史, 加藤 翼, 丹田 奈緒子, 飯久保 正弘, 小関 健由

    東北大学歯学雑誌　37/38/39　(2/1-2/1)　47-47　2020/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  11. 周術期口腔支援センター・予防歯科におけるハイドロゲル創傷被覆・保護材(エピシル口腔溶液)の使用経験

    岩永 賢二郎, 丹田 奈緒子, 百々 美奈, 石河 理紗, 飯嶋 若菜, 加藤 翼, 渡辺 俊吾, 熊坂 晃, 伊藤 彩, 佐藤 聡子, 飯久保 正弘, 小関 健由

    東北大学歯学雑誌　37/38/39　(2/1-2/1)　52-52　2020/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  12. 周術期口腔支援センター・予防歯科におけるハイドロゲル創傷被覆・保護材(エピシル口腔溶液)の使用経験

    岩永 賢二郎, 丹田 奈緒子, 百々 美奈, 石河 理紗, 飯嶋 若菜, 加藤 翼, 渡辺 俊吾, 熊坂 晃, 伊藤 彩, 佐藤 聡子, 飯久保 正弘, 小関 健由

    東北大学歯学雑誌　37/38/39　(2/1-2/1)　52-52　2020/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  13. 治療計画用CT装置Siemens SOMATOM Definition AS+の初期使用経験

    武田 一也, 山本 貴也, 伊藤 謙吾, 神宮 啓一, 佐藤 清和, 小関 健由

    Japanese Journal of Radiology　38　(Suppl.)　9-9　2020/02

    Publisher: (公社)日本医学放射線学会

    ISSN： 1867-1071

    eISSN： 1867-108X

  14. Association of cleft lip and palate on mother-to-infant bonding: a cross-sectional study in the Japan Environment and Children's Study (JECS). Peer-reviewed

    Tsuchiya S, Tsuchiya M, Momma H, Koseki T, Igarashi K, Nagatomi R, Arima T, Yaegashi N, Japan Environment, Children’s Study Group

    BMC pediatrics　19　(1)　505　2019/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1186/s12887-019-1877-9 　

    eISSN： 1471-2431

  15. Professional Oral Care Reduces Carcinogenic Acetaldehyde Levels in Mouth Air of Perioperative Esophageal Cancer Patients: A Prospective Comparative Study. Peer-reviewed

    Tanda N, Washio J, Kamei T, Akazawa K, Takahashi N, Koseki T

    The Tohoku journal of experimental medicine　249　(1)　75-83　2019/09

    DOI： 10.1620/tjem.249.75 　

    ISSN： 0040-8727

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    Acetaldehyde is a potential carcinogen for esophageal cancer, and some oral microorganisms produce acetaldehyde from ethanol or glucose. In this prospective study, we examined the influence of professional oral care on acetaldehyde levels in mouth air of esophageal cancer patients. Acetaldehyde concentrations in mouth air and breath were measured by a portable gas chromatograph, and acetaldehyde production from oral microbiota was also evaluated. Samples were taken from 21 esophageal cancer patients (median age 68 years) and 20 age-matched healthy volunteers (control group) before and after oral care. Post-operative samples were also taken from 17 patients who had undergone surgery. All samples (mouth air, breath, and saliva) were collected 2 to 3 hours after lunch. Oral microbial samples were prepared from saliva. Genotype analysis of alcohol dehydrogenase 1B (ADH1B) and aldehyde dehydrogenase-2 (ALDH2) genes revealed no significant differences in the genotypes between the two groups. In the control group, acetaldehyde levels in mouth air showed no significant changes after oral care, while the amount of microbial acetaldehyde production from ethanol was significantly decreased. By contrast, among the patients, acetaldehyde levels in mouth air were significantly decreased after oral care and after operation, while the amount of microbial acetaldehyde production from ethanol showed no significant changes. Moreover, microbial acetaldehyde production from glucose was significantly decreased after operation. Overall, oral health was poorer in the patient group. In conclusion, professional oral care for esophageal cancer patients is effective for reducing acetaldehyde levels in mouth air due to the reduction of microbial count.

  16. Lactate dehydrogenase C is required for the protein expression of a sperm-specific isoform of lactate dehydrogenase A. International-journal Peer-reviewed

    Mina Dodo, Hiroshi Kitamura, Hiroki Shima, Daisuke Saigusa, Sisca Meida Wati, Nao Ota, Fumiki Katsuoka, Hatsune Chiba, Hiroaki Okae, Takahiro Arima, Kazuhiko Igarashi, Takeyoshi Koseki, Hiroki Sekine, Hozumi Motohashi

    Journal of biochemistry　165　(4)　323-334　2019/04/01

    DOI： 10.1093/jb/mvy108 　

    ISSN： 0021-924X

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    Metabolites are sensitive indicators of moment-to-moment cellular status and activity. Expecting that tissue-specific metabolic signatures unveil a unique function of the tissue, we examined metabolomes of mouse liver and testis and found that an unusual metabolite, 2-hydroxyglutarate (2-HG), was abundantly accumulated in the testis. 2-HG can exist as D- or L-enantiomer, and both enantiomers interfere with the activities of 2-oxoglutarate (2-OG)-dependent dioxygenases, such as the Jumonji family of histone demethylases. Whereas D-2-HG is produced by oncogenic mutants of isocitrate dehydrogenases (IDH) and known as an oncometabolite, L-2-HG was the major enantiomer detected in the testis, suggesting that a distinct mechanism underlies the testicular production of this metabolite. We clarified that lactate dehydrogenase C (LDHC), a testis-specific lactate dehydrogenase, is responsible for L-2-HG accumulation by generating and analysing Ldhc-deficient mice. Although the inhibitory effects of 2-HG on 2-OG-dependent dioxygenases were barely observed in the testis, the LDHA protein level was remarkably decreased in Ldhc-deficient sperm, indicating that LDHC is required for LDHA expression in the sperm. This unique functional interaction between LDH family members supports lactate dehydrogenase activity in the sperm. The severely impaired motility of Ldhc-deficient sperm suggests a substantial contribution of glycolysis to energy production for sperm motility.

  17. Exhaled acetone and isoprene in perioperative lung cancer patients under intensive oral care: possible indicators of inflammatory responses and metabolic changes Peer-reviewed

    Tanda N, Hoshikawa Y, Sato T, Takahashi N, Koseki T

    Biomedical Research　40　(1)　29-36　2019/02

    DOI： 10.2220/biomedres.40.29 　

  18. 舌癌に対する198Au粒子での小線源治療における周術期口腔管理の器具として設計したスペーサー(Design of the spacer for brachytherapy using 198Au grain for carcinoma of the tongue as a tool of perioperative oral management) Peer-reviewed

    Suenaga Hanako, Tamahara Toru, Dodo Mina, Murakami Toshimitsu, Sasaki Satoshi, Ito Kengo, Jingu Keiichi, Koseki Takeyoshi, Sasaki Keiichi

    Journal of Prosthodontic Research　62　(4)　518-521　2018/10

    Publisher: (公社)日本補綴歯科学会

    DOI： 10.1016/j.jpor.2018.07.002 　

    ISSN： 1883-1958

    eISSN： 1883-9207

  19. 頭頸部腫瘍患者における治療前後の口腔機能変化に関する検討

    村上 裕理亜, 佐藤 由美子, 渡部 千代, 小関 健由, 泉田 一賢, 水戸 武彦, 佐藤 奈央子, 小山 重人

    日本歯科衛生学会雑誌　13　(1)　112-112　2018/08

    Publisher: 日本歯科衛生学会

    ISSN： 1884-5193

  20. 大学病院摂食嚥下治療センターにおける歯科衛生士の取り組みについて

    佐藤 由美子, 村上 裕理亜, 渡部 千代, 小関 健由, 泉田 一賢, 水戸 武彦, 佐藤 奈央子, 小山 重人

    日本歯科衛生学会雑誌　13　(1)　123-123　2018/08

    Publisher: 日本歯科衛生学会

    ISSN： 1884-5193

  21. Factors associated with sports-related dental injuries among young athletes: a cross-sectional study in Miyagi prefecture. International-journal Peer-reviewed

    Shinobu Tsuchiya, Masahiro Tsuchiya, Haruki Momma, Takuya Sekiguchi, Kaoru Kuroki, Kenji Kanazawa, Takeyoshi Koseki, Kaoru Igarashi, Ryoichi Nagatomi, Yoshihiro Hagiwara

    BMC oral health　17　(1)　168-168　2017/12/29

    DOI： 10.1186/s12903-017-0466-2 　

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    BACKGROUND: Sports-related dental injuries, such as tooth fracture, loosening, and avulsion, are a major concern among young athletes because they directly impair oral function. Although the preventive efficacy of mouthguards has been well established, the prevalence of sports-related dental injuries remains high among young athletes. The aim of this study is to identify the variables contributing to the risk of sports-related dental injuries by conducting a survey on large population of young athletes in Miyagi prefecture. METHODS: A cross-sectional study was conducted with school-aged athletes (aged 6-15 years, n = 5735) using a self-reported questionnaire. The questionnaire examined general variables, including sex, age, and body mass index; sports-related variables, including sports-type, team level, activity schedule, break time, and verbal/physical abuse by coaches; and lifestyle variables related to free time, including screen-time and sleep duration. Their associations with sports-related dental injuries were examined using multivariate logistic regression models. RESULTS: The prevalence of sports-related dental injuries was 13.3% (763 of 5735 young athletes) and was higher in males (14.3%, 592 of 4132) than in females (10.7%, 171 of 1603; adjusted odds ratios [ORs] and 95% confidence intervals [CIs]: 1.48 [1.22-1.79], p < 0.001). After stratification according to sex, significant associations with the prevalence of sports-related dental injuries were evident for three variables-insufficient break time, verbal abuse, and physical punishment-in males (adjusted ORs [95% CI]: 1.35 [1.03-1.77], p = 0.032; 1.31 [1.05-1.62], p = 0.015; and 1.36 [1.06-1.75], p = 0.016, respectively) but not in females (adjusted ORs [95% CI]: 0.88 [0.53-1.47], p = 0.623; 1.29 [0.87-1.91], p = 0.206; and 0.97 [0.57-1.63], p = 0.894, respectively). CONCLUSIONS: Although our results might be based on the individual athlete's self-perception to the sports-related variables, our results suggest that insufficient break time, verbal abuse, and physical punishment from coaches are positively associated with the prevalence of sports-related dental injuries in young male athletes.

  22. The mTOR-Bach2 Cascade Controls Cell Cycle and Class Switch Recombination during B Cell Differentiation Peer-reviewed

    Toru Tamahara, Kyoko Ochiai, Akihiko Muto, Yukinari Kato, Nicolas Sax, Mitsuyo Matsumoto, Takeyoshi Koseki, Kazuhiko Igarashi

    MOLECULAR AND CELLULAR BIOLOGY　37　(24)　2017/12

    DOI： 10.1128/MCB.00418-17 　

    ISSN： 0270-7306

    eISSN： 1098-5549

  23. Metastasis in the mandibular condyle: A case report Peer-reviewed

    Mina Dodo, Masahiro Kumagai, Yuta Kato, Hisashi Hirakawa, Takeyoshi Koseki

    Journal of Medical Case Reports　11　(1)　323　2017/11/16

    Publisher: BioMed Central Ltd.

    DOI： 10.1186/s13256-017-1450-9 　

    ISSN： 1752-1947

  24. 2-ヒドロキシグルタル酸の精子形成・胎児発生における生理的役割の検討

    百々 美奈, 北村 大志, 太田 奈緒, 三枝 大輔, 勝岡 史城, 千葉 初音, 岡江 寛明, 有馬 隆博, 小関 健由, 本橋 ほづみ

    日本生化学会大会プログラム・講演要旨集　89回　[3P-165]　2016/09

    Publisher: (公社)日本生化学会

  25. 病院における多職種連携による口腔のケアと管理の推進について

    玉原 亨, 細川 亮一, 丹田 奈緒子, 末永 華子, 佐久間 陽子, 菅崎 将樹, 飯嶋 若菜, 百々 美奈, 加藤 翼, 渡辺 俊吾, 伊藤 恵美, 高橋 久美子, 小関 健由

    東北大学歯学雑誌　34/35　(2/1)　13-26　2016/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  26. Changes in Cognitive Functions in the Elderly Living in Temporary Housing after the Great East Japan Earthquake Peer-reviewed

    Aiko Ishiki, Shoji Okinaga, Naoki Tomita, Reiko Kawahara, Ichiro Tsuji, Ryoichi Nagatomi, Yasuyuki Taki, Takashi Takahashi, Masafumi Kuzuya, Shigeto Morimoto, Katsuya Iijima, Takeyoshi Koseki, Hiroyuki Arai, Katsutoshi Furukawa

    PLOS ONE　11　(1)　e0147025　2016/01

    DOI： 10.1371/journal.pone.0147025 　

    ISSN： 1932-6203

  27. Oral malodorous gases and oral microbiota: From halitosis to carcinogenesis Invited Peer-reviewed

    Tanda N, Hoshikawa Y, Ishida N, Sato T, Takahashi N, Hosokawa R, Koseki T

    Journal of Oral Biosciences　57　(4)　175-178　2015/11

  28. Analysis of ketone bodies in exhaled breath and blood of ten healthy Japanese at OGTT using a portable gas chromatograph Peer-reviewed

    Naoko Tanda, Yoshinori Hinokio, Jumpei Washio, Nobuhiro Takahashi, Takeyoshi Koseki

    JOURNAL OF BREATH RESEARCH　8　(4)　046008　2014/12

    DOI： 10.1088/1752-7155/8/4/046008 　

    ISSN： 1752-7155

    eISSN： 1752-7163

  29. Factors associated with dry mouth in dependent Japanese elderly Peer-reviewed

    Naoki Kakudate, Tsukasa Muramatsu, Mami Endoh, Kazuhito Satomura, Takeyoshi Koseki, Yuji Sato, Kayoko Ito, Tadashi Ogasawara, Seiji Nakamura, Etsuo Kishimoto, Haruhiko Kashiwazaki, Yoshihisa Yamashita, Kimio Uchiyama, Tatsuji Nishihara, Yutaka Kiyohara, Yasuaki Kakinoki

    GERODONTOLOGY　31　(1)　11-18　2014/03

    DOI： 10.1111/j.1741-2358.2012.00685.x 　

    ISSN： 0734-0664

    eISSN： 1741-2358

  30. Model of oral health care programs to achieve self-actualization in nursing homes

    Miyuki Sugiyama, Jun Aida, Takeyoshi Koseki

    Interface Oral Health Science 2011　306-308　2012/01/01

    DOI： 10.1007/978-4-431-54070-0_91 　

  31. Collaboration of Medical and Dental Facilities Promotes Oral Health Care in Hospitals Peer-reviewed

    Jun Harako, Toru Tamahara, Ryoichi Hosokawa, Kazunori Kimura, Moritoshi Komagata, Koji Hanaoka, Seiichi Aonuma, Fusako Okuya, Kaoru Itagaki, Yukiko Akai, Kazuhiko Tamura, Hidetoshi Sonobe, Takeyoshi Koseki

    Interface Oral Health Science 2011　297-299　2012

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_88 　

  32. Promotion of Professional Oral Care for Hospitalized and Home-Care Patients Peer-reviewed

    Toru Tamahara, Jun Harako, Ryoichi Hosokawa, Kazunori Kimura, Moritoshi Komagata, Koji Hanaoka, Seiichi Aonuma, Fusako Okuya, Kaoru Itagaki, Yukiko Akai, Kazuhiko Tamura, Hidetoshi Sonobe, Takeyoshi Koseki

    Interface Oral Health Science 2011　309-311　2012

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_92 　

  33. 口腔環境を考慮した糖負荷試験時呼気中アセトン測定と血中ケトン体との関連

    丹田 奈緒子, 檜尾 好徳, 由浪 有希子, 鷲尾 純平, 高橋 信博, 小関 健由

    糖尿病　54　(Suppl.1)　S-216　2011/04

    Publisher: (一社)日本糖尿病学会

    ISSN： 0021-437X

    eISSN： 1881-588X

  34. 東北6県における集団フッ化物洗口の実態2010

    田浦 勝彦, 鍛治山 徹, 五十嵐 雄一, 田村 光平, 佐藤 勤一, 斎藤 愼一, 栃内 圭子, 黒田 雅行, 田口 千恵子, 木本 一成, 境 脩, 相田 潤, 玉原 亨, 原子 惇, 鈴木 淳, 細川 亮一, 小関 健由

    みちのく歯学会雑誌　41　(1-2)　71-72　2010/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  35. フッ化物洗口の都道府県別にみた普及の推移 国の政策が果たした役割の検討

    田浦 勝彦, 相田 潤, 安藤 雄一, 晴佐久 悟, 田口 千恵子, 木本 一成, 葭原 明弘, 筒井 昭仁, 眞木 吉信, 荒川 浩久, 飯島 洋一, 磯崎 篤則, 小林 清吾, 小関 健由

    口腔衛生学会雑誌　60　(5)　556-562　2010/10

    Publisher: (一社)日本口腔衛生学会

    DOI： 10.5834/jdh.60.5_556 　

    ISSN： 0023-2831

  36. 入院中に口腔環境改善を試みた治療コンプライアンスの低い2型糖尿病の1例

    丹田 奈緒子, 檜尾 好徳, 由浪 有希子, 鷲尾 純平, 高橋 信博, 小関 健由, 片桐 秀樹, 岡 芳知

    糖尿病　53　(7)　528-528　2010/07

    Publisher: (一社)日本糖尿病学会

    ISSN： 0021-437X

    eISSN： 1881-588X

  37. National survey on the school-based fluoride mouth rinsing program in Japan: proposition regarding final assessment of Healthy Japan 21 in 2010, and in 2020 Peer-reviewed

    Katsuhiko Taura, Kazunari Kimoto, Satoru Haresaku, Osamu Sakai, Takeyoshi Koseki

    INTERFACE ORAL HEALTH SCIENCE 2009　353-+　2010

    DOI： 10.1007/978-4-431-99644-6_105 　

  38. Tonometric measurement of the gingiva in young and elder humans Peer-reviewed

    Kyoko Ikawa, Motohide Ikawa, Takeyoshi Koseki

    INTERFACE ORAL HEALTH SCIENCE 2009　181-+　2010

    DOI： 10.1007/978-4-431-99644-6_41 　

  39. Difference between age generation of oral health examination in a rural town Peer-reviewed

    Naoko Tanda, Kyoko Ikawa, Jumpei Washio, Yoshiko Shigihara, Yoshiro Shibuya, Masaki Iwakura, Megumi Naga, Yuhei Ogawa, Katsuhiko Taura, Takeyoshi Koseki

    INTERFACE ORAL HEALTH SCIENCE 2009　323-+　2010

    DOI： 10.1007/978-4-431-99644-6_94 　

  40. A new intra-oral pressure monitor for screening swallowing dysfunction Peer-reviewed

    Tatsuo Aoba, Jun Suzuki, Naoko Tanda, Kyoko Ikawa, Katsuhiko Taura, Emi Ito, Takeyoshi Koseki

    INTERFACE ORAL HEALTH SCIENCE 2009　356-357　2010

    DOI： 10.1007/978-4-431-99644-6_106 　

  41. 東北大学病院附属歯科医療センターにおける特定機能病院へ向けての取り組み

    佐々木 啓一, 島内 英俊, 菊池 雅彦, 小関 健由, 五十嵐 薫

    みちのく歯学会雑誌　40　(1-2)　19-19　2009/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  42. Differences of Caries Experience between Nursery School Children and Kindergarten Children : Analysis on new dental examination system

    IGARI Kazuko, KOSEKI Takeyoshi, TSUKADA Mitsuo, AMANO Mieko, KOSUGA Akira, FUKUMOTO Satoshi

    The Japanese Journal of Pediatric Dentistry　47　(3)　427-432　2009/06/25

    Publisher: Japanese Society of Pediatric Dentistry

    DOI： 10.11411/jspd.47.3_427 　

    ISSN： 0583-1199

    More details Close

    We constructed a new dental examination using standardized examination criteria and data analysis systems in order to assess the status of caries experiences of preschool children in S city. In 2007, clinical dental examinations on the new system were carried out at total of 118 nursery schools and 106 kindergartens in S city. A total of 23,222 childrens data (10,432 nursery school children and 12,790 kindergarten children) was obtained, and caries prevalence and status of treatment were compared between nursery school children and kindergarten children. The following results were obtained. 1. Percentage of children with caries was 29.6% for 3-year-olds, 44.1% for 4-year-olds, 56.1% for 5- year-olds in nursery schools, whereas it was 24.3% for 3-year-olds, 39.8% for 4-year-olds, 52.0% for 5-year-olds in kindergartens. Those were significantly higher in nursery schools than in kindergartens. 2. Mean dft values per child at 3 to 5-years-old in nursery school children were also higher than those in kindergarten children. 3. Trend toward decrease in caries prevalence was showed in both nursery school children and kindergarten children, but it was 2 to 3 years behind the national trend. 4. Percentage of children with caries experience and without decayed teeth and percentage of treated teeth at each age of 3 to 5-years-old in nursery school children was significantly lower than those in kindergarten children. 5. Percentage of treated teeth for all age children in both nursery schools and kindergartens except 5- year-old children in kindergartens was far lower than the percentage of decayed teeth. These results demonstrated that both nursery school children and kindergarten children in S city still showed high caries experiences and nursery school children had higher caries prevalence and more untreated teeth than kindergarten children.

  43. 某高等専門学校男子学生における1975年から2005年のう蝕有病状況の改善について

    田浦 勝彦, 山田 雄大, 鈴木 淳, 伊藤 恵美, 井川 恭子, 丹田 奈緒子, 鷲尾 純平, 小関 健由

    東北大学歯学雑誌　28　(1)　13-17　2009/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  44. 介護予防「口腔機能向上」プログラム対象者選定項目と歯科医療ニーズとの関連―要介護者を対象とした分析― Peer-reviewed

    野口有紀, 相田潤, 丹田奈緒子, 伊藤恵美, 金高弘恭, 小関健由, 小坂健

    口腔衛生学会雑誌　59　(2)　111-117　2009/04

    Publisher:

    DOI： 10.5834/jdh.59.2_111 　

    ISSN： 0023-2831

  45. 顎変形症手術症例クリニカルパスへの連携とその課題

    丹田 奈緒子, 田浦 勝彦, 斎藤 恵一, 井川 恭子, 後藤 哲, 小枝 聡子, 鈴木 孝裕, 根井 仁志, 原 純一, 高田 陽子, 川村 仁, 小関 健由

    東北大学歯学雑誌　27　(1)　30-30　2008/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  46. 口腔育成参加患者のう蝕抑制効果について

    井川 恭子, 門馬 祐子, 千葉 美麗, 田浦 勝彦, 齋藤 恵一, 畑 真二, 吉田 光秀, 丸谷 由里子, 高橋 一郎, 台丸谷 隆慶, 富塚 亮, 福本 敏, 山本 照子, 小関 健由

    東北大学歯学雑誌　27　(1)　28-28　2008/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  47. MRL/lprマウスの唾液腺炎におけるAquaporin 5,SSA,ssDNAの発現に対する緑茶カテキン投与の影響

    齋藤 恵一, 森 士朗, 小関 健由

    東北大学歯学雑誌　27　(1)　27-27　2008/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  48. Heparin inhibits osteoclastic differentiation and function Peer-reviewed

    Wataru Ariyoshi, Tetsu Takahashi, Takahiro Kanno, Hisashi Ichimiya, Kouhei Shinmyouzu, Hiroshi Takano, Takeyoshi Koseki, Tatsuji Nishihara

    JOURNAL OF CELLULAR BIOCHEMISTRY　103　(6)　1707-1717　2008/04

    DOI： 10.1002/jcb.21559 　

    ISSN： 0730-2312

  49. 某高等専門学校男子学生の齲蝕の減少について 15歳と19歳の1975年から2005年までの成績から

    山田 雄大, 小川 裕平, 天野 一宇, 丹田 奈緒子, 井川 恭子, 田浦 勝彦, 鷲尾 純平, 小関 健由

    東北大学歯学雑誌　26　(2)　43-44　2007/12

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  50. 某高等専門学校男子学生の齲蝕有病状況について 15歳と19歳の1975年から2005年までの変化

    田浦 勝彦, 井川 恭子, 山田 雄大, 小川 裕平, 天野 一宇, 丹田 奈緒子, 鷲尾 純平, 小関 健由

    口腔衛生学会雑誌　57　(3)　228-228　2007/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  51. 町の一般健診に併設して導入に口臭を用いた学習歯科健診

    蒲生 正若, 蒲生 裕子, 木村 篤, 木村 歩, 吉田 厚子, 玉根 幸恵, 松本 百合恵, 高原 カネ子, 井川 恭子, 丹田 奈緒子, 草野 歩, 山田 雄大, 小川 裕平, 天野 一宇, 鴫原 ヨシ子, 伊藤 恵美, 鷲尾 純平, 渋谷 芳郎, 岩倉 政城, 小関 健由

    口腔衛生学会雑誌　57　(3)　228-229　2007/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  52. A new portable sulfide monitor with a zinc-oxide semiconductor sensor for daily use and field study Peer-reviewed

    Naoko Tanda, Jumpei Washio, Kyoko Ikawa, Kengo Suzuki, Takeyoshi Koseki, Masaki Iwakura

    JOURNAL OF DENTISTRY　35　(7)　552-557　2007/07

    DOI： 10.1016/j.jdent.2007.03.003 　

    ISSN： 0300-5712

  53. 津山町で実施した学習歯科健診の試み

    丹田 奈緒子, 岩倉 政城, 井川 恭子, 天野 一宇, 小川 裕平, 山田 雄大, 草野 歩, 鷲尾 純平, 鴫原 ヨシ子, 渋谷 芳郎, 小関 健由, 阿部 陽子, 小橋 純子, 渋谷 香奈, 小泉 香織, 成澤 喜美子, 芳賀 めぐみ, 小坂 健

    宮城県公衆衛生学会会誌　(39)　12-12　2007/03

    Publisher: 宮城県公衆衛生学会

    ISSN： 0912-747X

  54. Potential role of high molecular weight hyaluronan in the anti-Candida activity of human oral epithelial cells Peer-reviewed

    Akiyoshi Sakai, Sumio Akifusa, Naoki Itano, Koji Kimata, Taro Kawamura, Takeyoshi Koseki, Tadamichi Takehara, Tatsuji Nishihara

    MEDICAL MYCOLOGY　45　(1)　73-79　2007/02

    DOI： 10.1080/13693780601039607 　

    ISSN： 1369-3786

  55. 舌苔中の硫化水素産生菌の網羅的検索と菌種の同定 口臭との関連

    鷲尾 純平, 佐藤 拓一, 小関 健由, 高橋 信博

    Journal of Oral Biosciences　47　(Suppl.)　126-126　2005/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  56. Immunohistochemical study on the pathogenesis of drug-induced gingival hyperplasia Peer-reviewed

    Keiichi Saito, Shiro Mori, Masaki Iwakura, Naoko Tanda, Seizaburo Sakamoto, Kyoko Ikawa, Takeyoshi Koseki

    International Congress Series　1284　85-86　2005/09

    DOI： 10.1016/j.ics.2005.06.009 　

    ISSN： 0531-5131

  57. Influence of salivary mutans streptococci level and HLA gene polymorphisms to dental caries susceptibility Peer-reviewed

    J. Chiba, Y. Ozawa, T. Matsusaka, N. Tanda, T. Koseki

    International Congress Series　1284　181-182　2005/09

    DOI： 10.1016/j.ics.2005.06.094 　

    ISSN： 0531-5131

  58. Circadian changes of volatile sulfur compounds measured by Breathtron™ Peer-reviewed

    K. Ikawa, M. Iwakura, J. Washio, A. Kusano, N. Tanda, T. Koseki

    International Congress Series　1284　89-90　2005/09

    DOI： 10.1016/j.ics.2005.06.008 　

    ISSN： 0531-5131

  59. Development of a portable bad-breath monitor and application to field study of halitosis Peer-reviewed

    Naoko Tanda, Masaki Iwakura, Kyoko Ikawa, Junpei Washio, Ayumi Kusano, Kengo Suzuki, Takeyoshi Koseki

    International Congress Series　1284　201-202　2005/09

    DOI： 10.1016/j.ics.2005.06.064 　

    ISSN： 0531-5131

  60. Relationship between hydrogen sulfide-producing bacteria of the tongue coating and oral malodor Peer-reviewed

    J. Washio, T. Sato, K. Ikawa, N. Tanda, M. Iwakura, T. Koseki, N. Takahashi

    International Congress Series　1284　199-200　2005/09

    DOI： 10.1016/j.ics.2005.06.068 　

    ISSN： 0531-5131

  61. Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodour Peer-reviewed

    J Washio, T Sato, T Koseki, N Takahashi

    JOURNAL OF MEDICAL MICROBIOLOGY　54　(9)　889-895　2005/09

    DOI： 10.1099/jmm.0.46118-0 　

    ISSN： 0022-2615

  62. 口臭測定の導入が地域の歯科保健活動に果たす役割

    丹田 奈緒子, 岩倉 政城, 井川 恭子, 鷲尾 純平, 草野 歩, 天野 一宇, 今泉 芙由子, 小川 裕平, 山田 雄大, 石井 瑛一郎, 渋谷 芳郎, 芳賀 めぐみ, 鴫原 ヨシ子, 鈴木 健吾, 小関 健由

    口腔衛生学会雑誌　55　(4)　330-330　2005/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  63. 某高等専門学校生のう蝕の状況と歯科受診,フッ化物配合歯磨き剤の使用状況との関係について

    田浦 勝彦, 山田 雄大, 小川 裕平, 天野 一宇, 石井 瑛一郎, 今泉 芙由子, 千葉 順子, 丹田 奈緒子, 井川 恭子, 松坂 朋典, 鷲尾 純平, 小関 健由

    口腔衛生学会雑誌　55　(4)　424-424　2005/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  64. 日本における小学校教科書「保健」(5・6年)と「ほけん」(3・4年)の口腔関連記述について

    田浦 勝彦, 木本 一成, 志村 匡代, 晴佐久 悟, 荒川 浩久, 小林 清吾, 磯崎 篤則, 小関 健由

    口腔衛生学会雑誌　55　(4)　500-500　2005/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  65. 某高等専門学校生のう蝕罹患状況の改善について

    田浦 勝彦, 鷲尾 純平, 丹田 奈緒子, 井川 恭子, 山田 雄大, 小川 裕平, 天野 一宇, 今泉 芙由子, 石井 瑛一郎, 小関 健由

    口腔衛生学会雑誌　55　(3)　221-221　2005/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  66. 口臭測定の導入が地域歯科保健活動に果たす役割 BreathtronTMによる成人歯科健診受診者への一斉検査

    丹田 奈緒子, 岩倉 政城, 井川 恭子, 鷲尾 純平, 草野 歩, 山田 雄大, 小川 裕平, 天野 一宇, 今泉 芙由子, 石井 瑛一郎, 鈴木 健吾, 小関 健由

    口腔衛生学会雑誌　55　(3)　222-222　2005/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  67. Mechanisms involved in enhancement of osteoclast formation and function by low molecular weight hyaluronic acid Peer-reviewed

    W Ariyoshi, T Takahashi, T Kanno, H Ichimiya, H Takano, T Koseki, T Nishihara

    JOURNAL OF BIOLOGICAL CHEMISTRY　280　(19)　18967-18972　2005/05

    DOI： 10.1074/jbc.M412740200 　

    ISSN： 0021-9258

  68. Mechanisms involved in enhancement of osteoclast formation and function by low molecular weight hyaluronic acid Peer-reviewed

    W Ariyoshi, T Takahashi, T Kanno, H Ichimiya, H Takano, T Koseki, T Nishihara

    JOURNAL OF BIOLOGICAL CHEMISTRY　280　(19)　18967-18972　2005/05

    DOI： 10.1074/jbc.M412740200 　

    ISSN： 0021-9258

  69. 東北大学歯学部における口腔育成実習について

    千葉 美麗, 小沢 雄樹, 畑 真二, 高橋 一郎, 五十嵐 薫, 小関 健由, 真柳 秀昭

    東北大学歯学雑誌　23　(2)　89-90　2004/12

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  70. LiNbO3を用いた中赤外線波長レーザーの生物学的効果

    鈴木 麻衣子, 鷲尾 純平, 小関 健由

    東北大学歯学雑誌　23　(2)　90-90　2004/12

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  71. gamma-interferon enhances expression of CD14/MyD88 and subsequent responsiveness to lipopolysaccharide from Actinobacillus actinomycetemcomitans in human gingival fibroblasts Peer-reviewed

    S Mochizuki, M Kobayashi, T Suzuki, A Oikawa, T Koseki, T Nishihara, K Hasegawa

    JOURNAL OF PERIODONTAL RESEARCH　39　(5)　333-343　2004/10

    DOI： 10.1111/j.1600-0765.2004.00749.x 　

    ISSN： 0022-3484

  72. 3歳児のう蝕をきっかけに取り組んだ地域歯科保健計画策定と成人歯科健診

    芳賀 めぐみ, 鷲尾 純平, 井川 恭子, 丹田 奈緒子, 草野 歩, 渋谷 芳郎, 岩倉 政城, 小関 健由

    口腔衛生学会雑誌　54　(4)　372-372　2004/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  73. 低分子ヒアルロン酸の破骨細胞分化・骨吸収の増強作用

    有吉 渉, 管野 貴浩, 高野 裕史, 高橋 哲, 小関 健由, 西原 達次

    日本骨代謝学会学術集会プログラム抄録集　22回　195-195　2004/08

    Publisher: (一社)日本骨代謝学会

    ISSN： 1349-0761

  74. A new automatic device for measuring the spinnbarkeit of saliva: the Neva Meter Peer-reviewed

    K Gohara, T Ansai, T Koseki, M Ishikawa, Y Kakinoki, K Shibuya, T Nishihara, T Takehara

    JOURNAL OF DENTISTRY　32　(4)　335-338　2004/05

    DOI： 10.1016/j.jdent.2004.01.006 　

    ISSN： 0300-5712

  75. Breathtronを用いた口臭測定の条件についての検討

    井川 恭子, 鷲尾 純平, 丹田 奈緒子, 針生 ひろみ, 志村 匡代, 草野 歩, 岩倉 政城, 小関 健由

    口腔衛生学会雑誌　54　(2)　152-153　2004/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  76. 住民歯科保健学習組織構成員の助言を契機に始めた歯周病療養の体験報告

    広田 好子, 朝倉 みさ子, 鴫原 ヨシ子, 鷲尾 純平, 小関 健由, 岩倉 政城

    口腔衛生学会雑誌　54　(2)　156-156　2004/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  77. 地域活動体験を経た歯科衛生士の業務進展

    大沼 禎子, 安斉 深雪, 伊東 美香, 折越 文子, 片平 玲子, 鴫原 ヨシ子, 渡辺 淳子, 朝倉 みさ子, 鷲尾 純平, 小関 健由, 岩倉 政城

    口腔衛生学会雑誌　54　(2)　157-157　2004/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  78. Involvement of caspase activation through release of cytochrome c from mitochondria in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans Peer-reviewed

    H Kasai, K Yamamoto, T Koseki, M Yokota, T Nishihara

    FEMS MICROBIOLOGY LETTERS　233　(1)　29-35　2004/04

    DOI： 10.1016/j.femsle.2004.01.034 　

    ISSN： 0378-1097

  79. Involvement of caspase activation through release of cytochrome c from mitochondria in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans Peer-reviewed

    H Kasai, K Yamamoto, T Koseki, M Yokota, T Nishihara

    FEMS MICROBIOLOGY LETTERS　233　(1)　29-35　2004/04

    DOI： 10.1016/j.femsle.2004.01.034 　

    ISSN： 0378-1097

  80. Detection of subgingival calculus and dentine caries by laser fluorescence Peer-reviewed

    E Kurihara, T Koseki, K Gohara, T Nishihara, T Ansai, T Takehara

    JOURNAL OF PERIODONTAL RESEARCH　39　(1)　59-65　2004/02

    DOI： 10.1111/j.1600-0765.2004.00712.x 　

    ISSN： 0022-3484

  81. Microbial etiology of periodontitis Peer-reviewed

    T Nishihara, T Koseki

    PERIODONTOLOGY 2000　36　14-26　2004

    DOI： 10.1111/j.1600-0757.2004.03671.x 　

    ISSN： 0906-6713

  82. 農村地区における口臭治療の潜在的需要について 成人歯科健診における口臭検査と質問紙調査の検討 Peer-reviewed

    志村匡代, 岩倉政城, 井川恭子, 小関健由

    東北大学歯学雑誌　22　(2)　99-105　2003/12

    ISSN： 0287-3915

  83. In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by E6 siRNA Peer-reviewed

    M Yoshinouchi, T Yamada, M Kizaki, J Fen, T Koseki, Y Ikeda, T Nishihara, K Yamato

    MOLECULAR THERAPY　8　(5)　762-768　2003/11

    DOI： 10.1016/j.ymthe.2003.08.004 　

    ISSN： 1525-0016

  84. 宮城県内におけるフッ化物配合歯磨剤の利用状況について Peer-reviewed

    井川恭子, 田浦勝彦, 楠本雅子, 千葉順子, 針生ひろみ, 小関健由

    口腔衛生学会雑誌　53　(5)　554-563　2003/10

    Publisher: Japanese Society for Oral Health

    DOI： 10.5834/jdh.53.5_554 　

    ISSN： 0023-2831

    More details Close

    In this study, the use of fluoride toothpaste was surveyed by questionnaire in 2,188 subjects aged between 1 and 56 years in Sendai City, Miyagi Prefecture, for the purpose of the promotion of its use. The subjects were categorized as 1-3 year-old children, 4-5 year-old children, primary school children, their parents and vocational school students. The survey was carried out from April to June 2000 using a questionnaire prepared by the Committee for the Promotion of Fluoride Use of the Japanese Society for Dental Health, and the results were as follows : 1. Tooth brushing was performed once a day or more in 88.8% of the subjects. The percentages of the daily use of fluoride during brushing in 1-3 year-old children, 4-5 year-old children, primary school children, vocational school students and the parents were 13.4, 34.0, 52.3, 55.3 and 66.5 (%), respectively. 2. The parents and vocational school students used a larger amount of toothpaste and rinsed more frequently than younger subjects. There was a significant difference in the frequency of mouth rinsing after brushing among the groups, dependent on the amount of toothpaste used. 3. The first reason for toothpaste selection was the preventive effect on dental caries and the second was inclusion of fluoride. For infants, most of the parents did not use toothpaste because of the anxiety of swallowing toothpaste. The vocational school students selected toothpaste primarily because it was available at home. Our findings suggested that dental professionals should prepare guidelines for appropriate use of toothpaste and should recommend the use of fluoride-containing toothpaste to increase exposure to small amounts of fluoride.

  85. 口臭と舌苔中の総菌数及び硫化水素産生菌数との関係

    鷲尾 純平, 佐藤 拓一, 井川 恭子, 丹田 奈緒子, 岩倉 政城, 小関 健由, 高橋 信博

    口腔衛生学会雑誌　53　(4)　344-344　2003/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  86. Expression of endothelin-1 in gingival epithelial cells Peer-reviewed

    E Yamamoto, S Awano, T Koseki, T Ansai, T Takehara

    JOURNAL OF PERIODONTAL RESEARCH　38　(4)　417-421　2003/08

    ISSN： 0022-3484

  87. Growth/differentiation factor-5 induces growth arrest and apoptosis in mouse B lineage cells with modulation by Smad Peer-reviewed

    T Nakahara, K Tominaga, T Koseki, M Yamamoto, K Yamato, J Fukuda, T Nishihara

    CELLULAR SIGNALLING　15　(2)　181-187　2003/02

    DOI： 10.1016/S0898-6568(02)00088-8 　

    ISSN： 0898-6568

  88. Involvement of cytochromc c and caspascs in apoptotic cell death of human submandibular gland ductal cells induced by concanamycin A Peer-reviewed

    K Aiko, T Tsujisawa, T Koseki, S Hashimoto, Y Morimoto, T Amagasa, T Nishihara

    CELLULAR SIGNALLING　14　(8)　717-722　2002/08

    DOI： 10.1016/S0898-6568(02)00016-5 　

    ISSN： 0898-6568

  89. p53-independent expression of p21(CIP1/WAF1) in plasmacytic cells during G(2) cell cycle arrest induced by Actinobacillus actinomycetemcomitans cytolethal distending toxin Peer-reviewed

    T Sato, T Koseki, K Yamato, K Saiki, K Konishi, M Yoshikawa, Ishikawa, I, T Nishihara

    INFECTION AND IMMUNITY　70　(2)　528-534　2002/02

    DOI： 10.1128/IAI.70.2.528-534.2002 　

    ISSN： 0019-9567

  90. Role of TGF-beta family in osteoclastogenesis induced by RANKL Peer-reviewed

    T Koseki, Y Gao, N Okahashi, Y Murase, T Tsujisawa, T Sato, K Yamato, T Nishihara

    CELLULAR SIGNALLING　14　(1)　31-36　2002/01

    DOI： 10.1016/S0898-6568(01)00221-2 　

    ISSN： 0898-6568

  91. Possible involvement of protein kinases and Smad2 signaling pathways on osteoclast differentiation enhanced by activin A Peer-reviewed

    Y Murase, N Okahashi, T Koseki, K Itoh, N Udagawa, O Hashimoto, H Sugino, T Noguchi, T Nishihara

    JOURNAL OF CELLULAR PHYSIOLOGY　188　(2)　236-242　2001/08

    DOI： 10.1002/jcp.1113 　

    ISSN： 0021-9541

  92. Activation of the p21(CIP1/WAF1) promoter by bone morphogenetic protein-2 in mouse B lineage cells Peer-reviewed

    K Yamato, S Hashimoto, T Imamura, H Uchida, N Okahashi, T Koseki, A Ishisaki, M Kizaki, K Miyazono, Y Ikeda, T Nishihara

    ONCOGENE　20　(32)　4383-4392　2001/07

    DOI： 10.1038/sj.onc.1204572 　 10.1038/sj/onc/ 　

    ISSN： 0950-9232

  93. Involvement of caspases in apoptotic cell death of murine macrophages infected with Actinobacillus actinomycetemcomitans Peer-reviewed

    K Nonaka, A Ishisaki, N Okahashi, T Koseki, S Kato, M Muro, K Nakashima, T Nishihara, Y Kowashi

    JOURNAL OF PERIODONTAL RESEARCH　36　(1)　40-47　2001/02

    ISSN： 0022-3484

  94. Activin A and follistatin control osteoclast differentiation through a smad2 signaling pathway

    Murase, Y, Okahashi, N, Koseki, T, Itoh, K, Udagawa, N, Hashimoto, O, Sugino, H, Nishihara, T

    Journal of Cellular Physiology　188　236-242　2001

    DOI： 10.1002/jcp.1113 　

  95. Osteoclast differentiation is associated with transient upregulation of cyclin-dependent kinase inhibitors p21(WAF1/CIP1) and p27(KIP1) Peer-reviewed

    N Okahashi, Y Murase, T Koseki, T Sato, K Yamato, T Nishihara

    JOURNAL OF CELLULAR BIOCHEMISTRY　80　(3)　339-345　2001

    ISSN： 0730-2312

  96. An induced proximity model for NF-kappa B activation in the Nod1/RICK and RIP signaling pathways Peer-reviewed

    N Inohara, T Koseki, JM Lin, L del Peso, PC Lucas, FF Chen, Y Ogura, G Nunez

    JOURNAL OF BIOLOGICAL CHEMISTRY　275　(36)　27823-27831　2000/09

    DOI： 10.1074/jbc.M003415200 　

    ISSN： 0021-9258

  97. Bone morphogenetic protein-2 induces apoptosis in human myeloma cells with modulation of STAT3 Peer-reviewed

    C Kawamura, M Kizaki, K Yamato, H Uchida, Y Fukuchi, Y Hattori, T Koseki, T Nishihara, Y Ikeda

    BLOOD　96　(6)　2005-2011　2000/09

    ISSN： 0006-4971

  98. Dissociation of bone morphogenetic protein-mediated growth arrest and apoptosis of mouse B cells by HPV-16 E6/E7 Peer-reviewed

    K Yamato, S Hashimoto, N Okahashi, A Ishisaki, K Nonaka, T Koseki, M Kizaki, Y Ikeda, T Nishihara

    EXPERIMENTAL CELL RESEARCH　257　(1)　198-205　2000/05

    DOI： 10.1006/excr.2000.4876 　

    ISSN： 0014-4827

  99. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappa B Peer-reviewed

    N Inohara, T Koseki, L del Peso, YM Hu, C Yee, S Chen, R Carrio, J Merino, D Liu, J Ni, G Nunez

    JOURNAL OF BIOLOGICAL CHEMISTRY　274　(21)　14560-14567　1999/05

    DOI： 10.1074/jbc.274.21.14560 　

    ISSN： 0021-9258

  100. Oral administration of Porphyromonas gingivalis fimbriae with cholera toxin induces anti-fimbriae serum IgG, IgM, IgA and salivary IgA antibodies Peer-reviewed

    T Nagasawa, M Aramaki, N Takamatsu, T Koseki, H Kobayashi, Ishikawa, I

    JOURNAL OF PERIODONTAL RESEARCH　34　(3)　169-174　1999/04

    ISSN： 0022-3484

  101. CIPER, a novel NF kappa B-activating protein containing a caspase recruitment domain with homology to Herpesvirus-2 protein E10 Invited Peer-reviewed

    T Koseki, N Inohara, S Chen, R Carrio, J Merino, MO Hottiger, GJ Nabel, G Nunez

    JOURNAL OF BIOLOGICAL CHEMISTRY　274　(15)　9955-9961　1999/04

    DOI： 10.1074/jbc.274.15.9955 　

    ISSN： 0021-9258

  102. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD Peer-reviewed

    N Inohara, T Koseki, S Chen, MA Benedict, G Nunez

    JOURNAL OF BIOLOGICAL CHEMISTRY　274　(1)　270-274　1999/01

    DOI： 10.1074/jbc.274.1.270 　

    ISSN： 0021-9258

  103. Phylogenetic analysis of saccharolytic oral treponemes isolated from human subgingival plaque Peer-reviewed

    M Sakamoto, T Koseki, M Umeda, Ishikawa, I, Y Benno, T Nakase

    MICROBIOLOGY AND IMMUNOLOGY　43　(7)　711-716　1999

    ISSN： 0385-5600

  104. Actinobacillus actinomycetemcomitans toxin induces both cell cycle arrest in the G(2)/M phase and apoptosis Peer-reviewed

    M Ohguchi, A Ishisaki, N Okahashi, M Koide, T Koseki, K Yamato, T Noguchi, T Nishihara

    INFECTION AND IMMUNITY　66　(12)　5980-5987　1998/12

    ISSN： 0019-9567

  105. Diva, a Bcl-2 homologue that binds directly to Apaf-1 and induces BH3-independent cell death Peer-reviewed

    N Inohara, TS Gourley, R Carrio, M Muniz, J Merino, L Garcia, T Koseki, YM Hu, S Chen, G Nunez

    JOURNAL OF BIOLOGICAL CHEMISTRY　273　(49)　32479-32486　1998/12

    DOI： 10.1074/jbc.273.49.32479 　

    ISSN： 0021-9258

  106. The role of activin type I receptors in activin A-induced growth arrest and apoptosis in mouse B-cell hybridoma cells Peer-reviewed

    O Hashimoto, K Yamato, T Koseki, M Ohguchi, A Ishisaki, H Shoji, T Nakamura, Y Hayashi, H Sugino, T Nishihara

    CELLULAR SIGNALLING　10　(10)　743-749　1998/11

    DOI： 10.1016/S0898-6568(98)00021-7 　

    ISSN： 0898-6568

  107. Presence of activated B-1 cells in chronic inflamed gingival tissue Peer-reviewed

    M Aramaki, T Nagasawa, T Koseki, Ishikawa, I

    JOURNAL OF CLINICAL IMMUNOLOGY　18　(6)　421-429　1998/11

    ISSN： 0271-9142

  108. Two different types of humoral immune response to Actinobacillus actinomycetemcomitans in high-responder periodontitis patients Peer-reviewed

    K Nakashima, C Usui, T Koseki, T Nishihara, Ishikawa, I

    JOURNAL OF MEDICAL MICROBIOLOGY　47　(7)　569-575　1998/07

    ISSN： 0022-2615

  109. Correlation between Bcl-X expression and B-cell hybridoma apoptosis induced by activin A Invited Peer-reviewed

    T Koseki, K Yamato, A Ishisaki, O Hashimoto, H Sugino, T Nishihara

    CELLULAR SIGNALLING　10　(7)　517-521　1998/07

    DOI： 10.1016/S0898-6568(97)00191-5 　

    ISSN： 0898-6568

  110. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor Peer-reviewed

    N Inohara, T Koseki, S Chen, XY Wu, G Nunez

    EMBO JOURNAL　17　(9)　2526-2533　1998/05

    DOI： 10.1093/emboj/17.9.2526 　

    ISSN： 0261-4189

  111. RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis Peer-reviewed

    N Inohara, L del Peso, T Koseki, S Chen, G Nunez

    JOURNAL OF BIOLOGICAL CHEMISTRY　273　(20)　12296-12300　1998/05

    DOI： 10.1074/jbc.273.20.12296 　

    ISSN： 0021-9258

  112. Differentiation of clinical isolates of Actinobacillus actinomycetemcomitans using an insertion sequence, ISAa1. International-journal Peer-reviewed

    H Hayashida, H Hotokezaka, N Ohara, T Koseki, T Nishihara, O Takagi, T Yamada

    Oral microbiology and immunology　13　(2)　120-3　1998/04

    ISSN： 0902-0055

    More details Close

    We previously identified an IS200-like sequence (ISAa1) in the genome of Actinobacillus actinomycetemcomitans FDC Y4. One or more hybridizing bands to the ISAa1 probe were detected in each of several reference strains, representing three of the serotypes (a through c) of A. actinomycetemcomitans. In this study, we examined whether a restriction fragment-length polymorphism (RFLP) with ISAa1 as a probe could differentiate clinical isolates. One or more hybridizing bands were detected in each of the 27 strains examined, which could be divided into seven groups according to restriction fragment-length polymorphism pattern. Several strains were observed with identical restriction fragment-length polymorphism types but with different serotypes. Conversely, strains were also observed with differing restriction fragment-length polymorphism types and identical serotypes.

  113. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases Invited Peer-reviewed

    T Koseki, N Inohara, S Chen, G Nunez

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　95　(9)　5156-5160　1998/04

    DOI： 10.1073/pnas.95.9.5156 　

    ISSN： 0027-8424

  114. Increase in Bcl-2 level promoted by CD40 ligation correlates with inhibition of B cell apoptosis induced by vacuolar type H+-ATPase inhibitor Peer-reviewed

    S Akifusa, M Ohguchi, T Koseki, K Nara, Semba, I, K Yamato, N Okahashi, R Merino, G Nunez, N Hanada, T Takehara, T Nishihara

    EXPERIMENTAL CELL RESEARCH　238　(1)　82-89　1998/01

    DOI： 10.1006/excr.1997.3848 　

    ISSN： 0014-4827

  115. Prevalence of Actinobacillus actinomycetemcomitans serotypes in Japanese patients with periodontitis Peer-reviewed

    M Yamamoto, T Nishihara, T Koseki, T He, K Yamato, YJ Zhang, K Nakashima, S Oda, Ishikawa, I

    JOURNAL OF PERIODONTAL RESEARCH　32　(8)　676-681　1997/11

    ISSN： 0022-3484

  116. CLARP, a death effector domain-containing protein interacts with caspase-8 and regulates apoptosis Peer-reviewed

    N Inohara, T Koseki, YM Hu, S Chen, G Nunez

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　94　(20)　10717-10722　1997/09

    DOI： 10.1073/pnas.94.20.10717 　

    ISSN： 0027-8424

  117. Activin a induction of cell-cycle arrest involves modulation of cyclin D2 and p21(CIP1/WAF1) in plasmacytic cells Peer-reviewed

    K Yamato, T Koseki, M Ohguchi, M Kizaki, Y Ikeda, T Nishihara

    MOLECULAR ENDOCRINOLOGY　11　(8)　1044-1052　1997/07

    DOI： 10.1210/me.11.8.1044 　

    ISSN： 0888-8809

  118. Role of CD14 molecules in internalization of Actinobacillus actinomycetemcomitans by macrophages and subsequent induction of apoptosis Peer-reviewed

    M Muro, T Koseki, S Akifusa, S Kato, Y Kowashi, Y Ohsaki, K Yamato, M Nishijima, T Nishihara

    INFECTION AND IMMUNITY　65　(4)　1147-1151　1997/04

    ISSN： 0019-9567

  119. Induction of the immune response to periodontopathic bacteria and its role in the pathogenesis of periodontitis Peer-reviewed

    Ishikawa, I, K Nakashima, T Koseki, T Nagasawa, H Watanabe, S Arakawa, H Nitta, T Nishihara

    PERIODONTOLOGY 2000　14　79-111　1997

    ISSN： 0906-6713

  120. Characterization of the immunodominant antigens of Porphyromonas gingivalis 381 in high-responder patients Peer-reviewed

    EA Boutsi, T Koseki, T Nishihara, Ishikawa, I

    ORAL MICROBIOLOGY AND IMMUNOLOGY　11　(4)　236-241　1996/08

    ISSN： 0902-0055

  121. [Activin A induces apoptotic cell death of myeloma cells] Peer-reviewed

    Yamato K, Koseki T, Kisaki M, Ikeda Y, Nishihara T

    Rinsho Ketsueki　37　(7)　564-573　1996/07

    Publisher: The Japanese Society of Hematology

    DOI： 10.11406/rinketsu.37.564 　

    ISSN： 0485-1439

  122. Detection frequencies and the colony-farming unit recovery of oral treponemes by different cultivation methods Invited Peer-reviewed

    T Koseki, Y Benno, YJ ZhangKoseki, M Umeda, Ishikawa, I

    ORAL MICROBIOLOGY AND IMMUNOLOGY　11　(3)　203-208　1996/06

    ISSN： 0902-0055

  123. Nutritional analysis and an enriched medium for fermentative treponemes isolated from subgingival plaque Invited Peer-reviewed

    T Koseki, Ishikawa, I, E Boutsi, T He, Y Benno

    ORAL MICROBIOLOGY AND IMMUNOLOGY　11　(3)　166-171　1996/06

    ISSN： 0902-0055

  124. ACTIN A-INDUCED APOPTOSIS IS SUPPRESSED BY BCL-2 Invited Peer-reviewed

    T KOSEKI, K YAMATO, S KRAJEWSKI, JC REED, Y TSUJIMOTO, T NISHIHARA

    FEBS LETTERS　376　(3)　247-250　1995/12

    DOI： 10.1016/0014-5793(95)01290-7 　

    ISSN： 0014-5793

  125. CHARACTERIZATION OF ORAL TREPONEMES ISOLATED FROM HUMAN PERIODONTAL POCKETS Invited Peer-reviewed

    T KOSEKI, ISHIKAWA, I, M UMEDA, Y BENNO

    ORAL MICROBIOLOGY AND IMMUNOLOGY　10　(5)　271-277　1995/10

    ISSN： 0902-0055

  126. SPECIFIC INHIBITORS OF VACUOLAR-TYPE H+-ATPASES INDUCE APOPTOTIC CELL-DEATH Peer-reviewed

    T NISHIHARA, S AKIFUSA, T KOSEKI, S KATO, M MURO, N HANADA

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　212　(1)　255-262　1995/07

    DOI： 10.1006/bbrc.1995.1964 　

    ISSN： 0006-291X

  127. INDUCTION OF APOPTOSIS IN B-LINEAGE CELLS BY ACTIVIN-A DERIVED FROM MACROPHAGES Peer-reviewed

    T NISHIHARA, Y OHSAKI, N UEDA, T KOSEKI, Y ETO

    JOURNAL OF INTERFERON AND CYTOKINE RESEARCH　15　(6)　509-516　1995/06

    ISSN： 1079-9907

  128. 歯周治療の長期観察について Peer-reviewed

    坂東薫, 小関健由, 杉山榮一

    日本歯周病学会会誌　37　(2)　384-391　1995/06

    Publisher: The Japanese Society of Periodontology

    DOI： 10.2329/perio.37.384 　

    ISSN： 0385-0110

    More details Close

    The purpose of this study was to investigate the effect of periodontal treatment and maintenance therapy of 10 years duration. The study population consisted of 1 juvenile, 2 rapidly progressive and 43 adult periodontitis patients. The average age of the patients was 42.3 years at the initial visit and the average duration of observation was 14.1 years. At the initial and most recent maintenance visits, the number of remaining teeth and the interproximal bone levels on X-ray films were measured. The average number of remaining teeth was 27.4 at baseline and 24.7 at final follow up. The total number of lost teeth during observation was 124, that is 2.7 per individual. Four patients had lost more than 0.6 teeth per year, 5 patients had lost 0.3 to 0.6 teeth and 37 patients had lost less than 0.3. Twenty maxillary second molars and 18 mandibular third molars were extracted. The extractions were commonly located in the molar region and bilaterally symmetrical patterns of extraction were observed. Bone loss was observed in some regions of the mouth at the most recent visit. However, our data suggest that, despite slight bone loss, periodontal therapy was effective if maintenance therapy was correctly performed and that such treatment modalities facilitate reaching the goal of the "8020" movement.

  129. Membrane-associated interleukin-1 on macrophages stimulated with Actinobacillus actinomycetemcomitans lipopolysaccharide induces osteoclastic bone resorption in vivo. International-journal Peer-reviewed

    Nishihara T, Ishihara Y, Koseki T, Boutsi E A, Senpuku H, Hanada N

    Cytobios　81　(327)　229-237　1995/04

    ISSN： 0011-4529

    More details Close

    The effect of paraformaldehyde-fixed murine macrophage P388D1 cells stimulated with Actinobacillus actinomycetemcomitans Y4 lipopolysaccharide (membrane-associated interleukin-1) on osteoclastic bone resorption was investigated. Both the number of osteoclasts and the bone resorption surfaces increased considerably along the inside of the marrow space of mouse calvaria when membrane-associated interleukin-1 mediated by A. actinomycetemcomitans Y4 lipopolysaccharide were injected into the subcutaneous tissues overlying the central calvaria of 4-week-old C3H/HeJ mice. Membrane-associated interleukin-1 caused hypercalcaemia when injected into C3H/HeJ mice twice a day for 2 days. The calvaria of C3H-HeJ mice injected with membrane-associated interleukin-1 contained less mineral than those of the control mice. These results suggest that membrane-associated interleukin-1 on macrophages stimulated with A. actinomycetemcomitans Y4 lipopolysaccharide plays an important role in inflammatory osteoclastic bone resorption in periodontal diseases.

  130. 最近の歯学 歯科保存 口腔内スピロヘータの生態と培養 Peer-reviewed

    小関健由, 石川烈

    口腔病学会雑誌　60　(3)　417-417　1993/09

  131. 歯周ポケット由来Treponema属の分離・同定に関する研究 Peer-reviewed

    小関健由

    口腔病学会雑誌　60　(1)　149-168　1993/03

    DOI： 10.5357/koubyou.60.149 　

  132. Papillon-Lefevre症候群患者2名の臨床的,免疫学的および細菌学的検査所見および治療経過について Peer-reviewed

    梅田誠, 張いしょう, 小関健由

    口腔病学会雑誌　57　(3)　430-440　1990/09

    DOI： 10.5357/koubyou.57.430 　

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Misc. 134

  1. 自習にて初等プロービング実習を推進するプロービング学習法について

    笹井真澄, 瀬名浩太郎, 石河理沙, 加藤翼, 丹田奈緒子, 小関健由

    口腔衛生学会雑誌　74　2024

    ISSN： 0023-2831

  2. 頭頸部癌皮弁移植術前にアムホテリシンB耐性を有する稀少カンジダ感染を生じた1例

    石河理紗, 瀬名浩太郎, 瀬名浩太郎, 加藤翼, 笹井真澄, 丹田奈緒子, 小関健由

    口腔衛生学会雑誌　74　2024

    ISSN： 0023-2831

  3. 歯科衛生士の離職防止と復職に関連する要因:ストレスモデルと歯科医師との意識の差

    相田潤, 相田潤, 草間太郎, 五十嵐彩夏, 小関健由, 小坂健, 人見早苗, 人見早苗, 渡部千代, 渡部千代

    口腔衛生学会雑誌　71　2021

    ISSN： 0023-2831

  4. 唾液タンパク組成と全身状態の関連性

    渡辺俊吾, 岩永賢二郎, 百々美奈, 飯嶋若菜, 石河理紗, 玉原亨, 加藤翼, 織部令奈, 丹田奈緒子, 小関健由

    口腔衛生学会雑誌　69　(増刊)　172-172　2019/04/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  5. 術後性開口障害患者にかいくんを用いた開口訓練の効果について

    田中篤史, 岩永賢二郎, 玉原亨, 百々美奈, 飯嶋若菜, 猪狩真奈, 渡辺俊吾, 加藤翼, 清信仁, 丹田奈緒子, 小関健由

    日本歯科医療管理学会雑誌　53　(1)　75　2018/06/15

    ISSN： 0387-5687

  6. 東北大学病院周術期口腔支援センターおよび予防歯科における診療の動向

    田中篤史, 岩永賢二郎, 玉原亨, 百々美奈, 飯嶋若菜, 猪狩真奈, 渡辺俊吾, 加藤翼, 丹田奈緒子, 小関健由

    みちのく歯学会雑誌　48　(1-2)　26‐27-27　2017/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  7. 東北大学病院周術期口腔支援センターおよび予防歯科における診療の動向

    田中 篤史, 岩永 賢二郎, 玉原 亨, 百々 美奈, 飯嶋 若菜, 猪狩 真奈, 渡辺 俊吾, 加藤 翼, 丹田 奈緒子, 小関 健由

    みちのく歯学会雑誌　48　(1-2)　26-27　2017/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  8. 東日本大震災後の東北大学病院口臭外来受診動態

    丹田 奈緒子, 玉原 亨, 佐久間 陽子, 飯嶋 若菜, 猪狩 真奈, 百々 美奈, 渡辺 俊吾, 田中 篤史, 波多野 悠夏, 伊藤 恵美, 高橋 久美子, 細川 亮一, 小関 健由

    口腔衛生学会雑誌　67　(4)　312-312　2017/10

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  9. 半夏瀉心湯による口腔粘膜炎抑制の新たな作用機序の報告

    玉原 亨, 丹田 奈緒子, 佐久間 陽子, 飯嶋 若菜, 猪狩 真奈, 百々 美奈, 渡辺 俊吾, 田中 篤史, 波田野 悠夏, 伊藤 恵美, 高橋 久美子, 細川 亮一, 小関 健由

    口腔衛生学会雑誌　67　(4)　312-312　2017/10

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  10. 仮想現実を用いた歯周診療技能修得教育システムの構築 手指の動作を収録する力学的臨床技能修得装置による歯科臨床技能教育への試みについて

    小関 健由, 木下 淳博, 品田 佳世子, 合場 千佳子, 高阪 利美, 眞木 吉信

    日本歯科衛生教育学会雑誌　8　(2)　101-101　2017/10

    Publisher: 日本歯科衛生教育学会

    ISSN： 2186-3881

  11. 東北大学病院周術期口腔支援センターにおける初診患者の動向

    百々美奈, 玉原亨, 飯嶋若菜, 猪狩真奈, 渡辺俊吾, 田中篤史, 加藤翼, 佐久間陽子, 丹田奈緒子, 山内健介, 半田慶介, 下田元, 小関健由, 小関健由, 小関健由, 細川亮一, 飯久保正弘

    東北大学歯学雑誌　35/36　(2/1)　76‐79-79　2017/06/30

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  12. 東北大学病院周術期口腔支援センターにおける初診患者の動向

    百々 美奈, 玉原 亨, 飯嶋 若菜, 猪狩 真奈, 渡辺 俊吾, 田中 篤史, 加藤 翼, 佐久間 陽子, 丹田 奈緒子, 山内 健介, 半田 慶介, 下田 元, 小関 健由, 細川 亮一, 飯久保 正弘

    東北大学歯学雑誌　35/36　(2/1)　76-79　2017/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  13. ITを活用した新しい歯科臨床技能教育の試み 技能修得における拡張現実の活用 見えない技能を見ながら学習

    小関 健由

    口腔衛生学会雑誌　67　(増刊)　57-57　2017/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  14. 東北大学病院周術期口腔支援センターにおける診療の動向

    百々 美奈, 玉原 亨, 渡辺 俊吾, 田中 篤史, 加藤 翼, 畠山 博之, 高橋 志麻, 石川 元洋, 高柳 久与, 丹田 奈緒子, 小関 健由

    口腔衛生学会雑誌　67　(増刊)　134-134　2017/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  15. 地域住民の口腔保健の実施状況の10年間の経時変化

    渡辺 俊吾, 玉原 亨, 百々 美奈, 田中 篤史, 加藤 翼, 畠山 博之, 高橋 志麻, 石川 元洋, 高柳 久与, 丹田 奈緒子, 小関 健由

    口腔衛生学会雑誌　67　(増刊)　135-135　2017/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  16. 看護学生の口腔の健康に関する実態調査

    佐藤 由記子, 小関 健由

    日本看護学会論文集: 看護教育　47　(47)　43-46　2017/03

    Publisher: 日本看護学会

    ISSN： 1347-8265

  17. 口腔保健の推進が支える超高齢社会のQOL

    小関 健由

    山形県公衆衛生学会講演集　43回　np2-np2　2017/03

    Publisher: 山形県公衆衛生学会

  18. 半夏瀉心湯は頭頸部化学放射線治療由来の口腔粘膜炎を抑制する

    玉原亨, 細川亮一, 丹田奈緒子, 佐久間陽子, 飯嶋若菜, 百々美奈, 伊藤恵美, 高橋久美子, 小関健由

    みちのく歯学会雑誌　47　(1-2)　81‐82-82　2016/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  19. 東北大学病院における摂食嚥下治療センターの取り組みと摂食・嚥下障害の実態調査

    石河 理紗, 小山 重人, 佐藤 奈央子, 細川 亮一, 松井 裕之, 松舘 芳樹, 加藤 健吾, 香取 幸夫, 小関 健由, 佐々木 啓一

    みちのく歯学会雑誌　47　(1-2)　44-46　2016/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  20. The role of dental team in perioperative management of oral function

    細川亮一, 細川亮一, 玉原亨, 小関健由

    Japanese Journal of Maxillo Facial Implants　15　(2)　71‐76-76　2016/08/25

    Publisher: (公社)日本顎顔面インプラント学会

    ISSN： 1347-894X

  21. 頭頸部がんに対する化学放射線療法に伴う口腔粘膜炎に対する半夏瀉心湯の基礎研究と臨床的効果

    細川亮一, 細川亮一, 玉原亨, 丹田奈緒子, 佐久間陽子, 飯嶋若菜, 百々美奈, 渡辺俊介, 伊藤恵美, 小関健由

    和漢医薬学会学術大会要旨集　33rd　102‐103-103　2016/08

    Publisher: (一社)和漢医薬学会

  22. 東北大学病院予防歯科におけるBRONJ患者の動向

    加藤翼, 細川亮一, 丹田奈緒子, 末永華子, 菅崎将樹, 佐久間陽子, 飯嶋若菜, 玉原亨, 百々美奈, 福井玲子, 山崎佐千子, 笠原千秋, 伊藤恵美, 高橋久美子, 手代木史枝, 馬目麻衣, 小関健由

    口腔衛生学会雑誌　66　(3)　345-345　2016/04/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  23. 東日本大震災前後の歯科医師の社会活動の取り組みについて

    杉山 美幸, 百々 美奈, 加藤 翼, 玉原 亨, 飯島 若菜, 末永 華子, 菅崎 将樹, 佐久間 陽子, 丹田 奈緒子, 伊藤 恵美, 高橋 久美子, 坪井 明人, 土谷 昌広, 細川 亮一, 小関 健由

    口腔衛生学会雑誌　66　(3)　345-346　2016/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  24. エコチル追加調査による妊娠中の夫婦の口腔保健状況について

    小関健由, 石川元洋, 玉原亨, 百々美奈, 加藤翼, 高橋久美子, 細川亮一, 丹田奈緒子, 末永華子, 飯島若菜, 菅崎将樹, 佐久間陽子, 伊藤恵美, 有馬隆博

    宮城県公衆衛生学会会誌　(48)　12-12　2016/03

    Publisher: 宮城県公衆衛生学会

    ISSN： 0912-747X

  25. 東日本大震災を経験した歯科医師の感想と提言について

    杉山 美幸, 玉原 亨, 百々 美奈, 加藤 翼, 渡辺 俊吾, 伊藤 恵美, 土谷 昌広, 坪井 明人, 小関 健由

    感性福祉研究所年報　(17)　261-269　2016/03

    Publisher: 東北福祉大学感性福祉研究所

    ISSN： 1344-9966

  26. 「赤ちゃんとママのむし歯予防教室」の歯科保健学習効果について 宮城県妊娠中からの歯科保健モデル事業から

    根本充康, 細谷仁憲, 山本壽一, 新沼康弘, 鈴木宏明, 戸田愼治, 佐藤文彦, 佐々木隆二, 森拓也, 細川亮一, 丹田奈緒子, 末永華子, 菅崎将樹, 佐久間陽子, 飯島若菜, 菊地瑞穂, 玉原亨, 百々美奈, 加藤翼, 伊藤恵美, 高橋久美子, 戸田康子, 小関健由

    みちのく歯学会雑誌　46　(1-2)　60‐61-61　2015/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  27. 東日本大震災被災地の震災後の学校歯科保健の動向について

    手代木史枝, 伊藤恵美, 戸田康子, 高橋久美子, 菅崎将樹, 佐久間陽子, 玉原亨, 百々美奈, 加藤翼, 末永華子, 丹田奈緒子, 細川亮一, 小関健由

    口腔衛生学会雑誌　65　(2)　258-258　2015/04/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  28. 東北大学病院における緩和医療に関する学生ならびに歯科医師に対する意識調査

    細川亮一, 伊藤恵美, 手代木史枝, 丹田奈緒子, 菅崎将樹, 末永華子, 百々美奈, 佐久間陽子, 加藤翼, 玉原亨, 小関健由

    口腔衛生学会雑誌　65　(2)　240-240　2015/04/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  29. 1歳6か月児の母親の育児時の歯科保健意識の変化とう蝕発生との関連

    戸田康子, 玉原亨, 伊藤恵美, 高橋久美子, 手代木史枝, 菅崎将樹, 佐久間陽子, 百々美奈, 加藤翼, 末永華子, 丹田奈緒子, 細川亮一, 小関健由

    口腔衛生学会雑誌　65　(2)　204　2015/04/30

    ISSN： 0023-2831

  30. 農業地帯住民の歯科保健に関する意識調査について

    百々美奈, 高橋文一, 木村俊春, 山崎達見, 庄司達雄, 野田清一, 伏見行則, 白石信也, 近江浩昭, 曽根憲一, 虻江勝, 久保圭, 渋谷芳和, 玉原亨, 小野瑞穂, 加藤翼, 細川亮一, 丹田奈緒子, 吉田英子, 伊藤恵美, 小関健由

    口腔衛生学会雑誌　65　(1)　55-56　2015/01/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  31. 東北大学病院におけるがん治療と歯科処置

    小野瑞穂, 玉原亨, 百々美奈, 加藤翼, 福井玲子, 原美里, 山崎佐千子, 鈴木寿子, 細川亮一, 丹田奈緒子, 吉田英子, 末永華子, 飯嶋若菜, 伊藤恵美, 小関健由

    口腔衛生学会雑誌　65　(1)　54-54　2015/01/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  32. 「赤ちゃんとママのむし歯予防」教室受講者の歯科保健に関する意識と行動―妊娠中からの歯科保健モデル事業報告から(3)―

    菊池瑞穂, 細川亮一, 丹田奈緒子, 末永華子, 菅崎将樹, 佐久間陽子, 飯島若菜, 玉原亨, 百々美奈, 加藤翼, 伊藤恵美, 高橋久美子, 手代木史枝, 細谷仁憲, 山本壽一, 新沼康弘, 鈴木宏明, 戸田愼治, 佐藤文彦, 佐々木隆二, 根本充康, 小関健由

    口腔衛生学会雑誌　65　(1)　59-59　2015/01/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  33. 子どもの歯科健康診断情報収録システムの開発とデータベース化の取り組み

    加藤翼, 伊藤恵美, 玉原亨, 百々美奈, 小野瑞穂, 細川亮一, 丹田奈緒子, 吉田英子, 末永華子, 飯嶋若菜, 小関健由

    口腔衛生学会雑誌　65　(1)　56-56　2015/01/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  34. 妊娠時と1歳6か月児の育児時の母親の歯科保健に関する意識と行動の変化―妊娠中からの歯科保健モデル事業報告から(2)―

    戸田康子, 細川亮一, 丹田奈緒子, 末永華子, 菅崎将樹, 佐久間陽子, 飯島若菜, 菊池瑞穂, 玉原亨, 百々美奈, 加藤翼, 伊藤恵美, 高橋久美子, 手代木史枝, 細谷仁憲, 山本壽一, 新沼康弘, 鈴木宏明, 戸田愼治, 佐藤文彦, 佐々木隆二, 根本充康, 小関健由

    口腔衛生学会雑誌　65　(1)　59　2015/01/30

    ISSN： 0023-2831

  35. 開口障害と多発性うしょくと放射線性顎骨壊死の重篤な晩期有害事象

    原美里, 笠原千秋, 山崎佐千子, 福井玲子, 山田桂子, 手代木史枝, 高橋久美子, 伊藤恵美, 細川亮一, 丹田奈緒子, 末永華子, 菅崎将樹, 佐久間陽子, 飯島若菜, 菊池瑞穂, 玉原亨, 百々美奈, 加藤翼, 小関健由

    口腔衛生学会雑誌　65　(1)　59-60　2015/01/30

    ISSN： 0023-2831

  36. 1歳6カ月児のうしょく発生に関与する妊娠中からの母親の意識と行動―妊娠中からの歯科保健モデル事業報告から(1)―

    根本充康, 細谷仁憲, 山本壽一, 新沼康弘, 鈴木宏明, 戸田愼治, 佐藤文彦, 佐々木隆二, 細川亮一, 丹田奈緒子, 末永華子, 菅崎将樹, 佐久間陽子, 飯島若菜, 菊池瑞穂, 玉原亨, 百々美奈, 加藤翼, 伊藤恵美, 高橋久美子, 手代木史枝, 戸田康子, 小関健由

    口腔衛生学会雑誌　65　(1)　58-59　2015/01/30

    ISSN： 0023-2831

  37. 口腔衛生関連用語に関する提言(第6回) 歯周疾患関連用語について

    小関 健由, 加藤 一夫, 佐藤 勉, 千葉 逸朗, 森田 学, 伊藤 博夫, 天野 敦雄, 齋藤 俊行, 廣瀬 公治, 山本 龍生, 川崎 弘二, 日本口腔衛生学会用語委員会

    口腔衛生学会雑誌　65　(1)　48-49　2015/01

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  38. 頭頸部癌に対する開口訓練の効果の検証

    吉田英子, 細川亮一, 丹田奈緒子, 末永華子, 菅崎将樹, 佐久間陽子, 飯嶋若菜, 菊池瑞穂, 玉原亨, 百々美奈, 加藤翼, 山崎佐千子, 原美里, 笠原千秋, 福井玲子, 伊藤恵美, 高橋久美子, 手代木史枝, 小関健由

    みちのく歯学会雑誌　45　(1-2)　7-8　2014/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  39. 東北大学病院予防歯科におけるがん患者の周術期口腔支援

    加藤翼, 細川亮一, 丹田奈緒子, 末永華子, 菅崎将樹, 佐久間陽子, 飯嶋若菜, 菊池瑞穂, 吉田英子, 玉原亨, 百々美奈, 山崎佐千子, 原美里, 笠原千秋, 福井玲子, 伊藤恵美, 高橋久美子, 手代木史枝, 小関健由

    みちのく歯学会雑誌　45　(1-2)　74-75　2014/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  40. 宮城県立精神医療センター入院患者における歯科保健指導方法の確立に向けた基礎調査

    小山 重人, 白石 成, 塙 総司, 細川 亮一, 小関 健由, 伊藤 恵美, 石河 理紗, 松井 裕之, 水戸 武彦, 貴田岡 亜希, 佐々木 啓一, 相田 潤, 小坂 健, 及川 祐司, 小高 晃

    みちのく歯学会雑誌　45　(1-2)　53-55　2014/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  41. 妊産婦の出産前後における口腔保健の行動・意識調査

    戸田康子, 伊藤恵美, 玉原亨, 加藤翼, 高橋久美子, 小関健由

    日本歯科衛生学会雑誌　9　(1)　217-217　2014/08/10

    Publisher: 日本歯科衛生学会

    ISSN： 1884-5193

  42. 糖尿病啓発イベントからみえる糖尿病と歯周病の関連

    山田 紗智子, 由浪 有希子, 児玉 栄一, 伊藤 恵美, 小関 健由, 清元 秀康, 石井 正

    宮城県糖尿病看護研究会　10回　13-13　2014/06

    Publisher: 宮城県糖尿病看護研究会

    ISSN： 2187-2511

  43. 口の健康と認知に関する歯科からの話題 健康の基は食べることと話すこと、口の健康から全身の健康へ

    小関 健由, 玉原 亨, 百々 美奈, 加藤 翼

    認知神経科学　16　(1)　1-8　2014/05

    Publisher: 認知神経科学会

    DOI： 10.11253/ninchishinkeikagaku.16.1 　

    ISSN： 1344-4298

  44. 東北大学病院におけるがん支持療法としての口腔機能管理の取り組み

    加藤翼, 細川亮一, 玉原亨, 吉田英子, 丹田奈緒子, 菅崎将樹, 末永華子, 百々美奈, 小野瑞穂, 伊藤恵美, 小関健由

    口腔衛生学会雑誌　64　(2)　235-235　2014/04/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  45. 妊産婦の出産前後における口腔保健の意識および行動の変化

    伊藤恵美, 玉原亨, 吉田英子, 丹田奈緒子, 細川亮一, 大内康宏, 小関健由

    日本歯科衛生学会雑誌　8　(1)　164-164　2013/08/10

    Publisher: 日本歯科衛生学会

    ISSN： 1884-5193

  46. 情報入力を省力化した歯科健診情報管理システムの構築

    伊藤恵美, 玉原亨, 細川亮一, 吉田英子, 丹田奈緒子, 小島健, 斉藤純一, 小関健由

    口腔衛生学会雑誌　63　(4)　362-363　2013/07/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  47. 脱水素酵素濃度により歯周疾患スクリーニングの年齢別成績

    平田正嗣, 玉原亨, 吉田英子, 梁川誠郎, 大山治, 山崎尚哉, 小菅玲, 大内康弘, 村上正博, 郷家茂樹, 木村一則, 高野友幸, 田鎖利明, 村上廣一, 馬場智彦, 細川亮一, 丹田奈緒子, 伊藤恵美, 小関健由, 長田純一

    口腔衛生学会雑誌　63　(4)　363-364　2013/07/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  48. 東北大学病院における周術期口腔機能管理

    吉田英子, 玉原亨, 細川亮一, 丹田奈緒子, 小島健, 福井玲子, 佐藤由美子, 高橋美里, 小野ゆかり, 伊藤恵美, 小関健由

    口腔衛生学会雑誌　63　(4)　364-364　2013/07/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  49. P-72 東北大学病院における口腔ケアの現状と症例(ポスター)

    吉田 英子, 玉原 亨, 細川 亮一, 丹田 奈緒子, 伊藤 恵美, 佐藤 由美子, 小関 健由

    口腔衛生学会雑誌　63　(2)　203-203　2013/04/30

    Publisher: 有限責任中間法人日本口腔衛生学会

    ISSN： 0023-2831

  50. P-74 歯科衛生士と看護師の協働の推進に関する質問紙調査(ポスター)

    吉田 志麻, 玉原 亨, 細川 亮一, 丹田 奈緒子, 吉田 英子, 伊藤 恵美, 小関 健由

    口腔衛生学会雑誌　63　(2)　204-204　2013/04/30

    Publisher: 有限責任中間法人日本口腔衛生学会

    ISSN： 0023-2831

  51. 東日本大震災による義歯喪失と口腔のQOLの関連

    佐藤 遊洋, 相田 潤, 竹内 研時, 若栗 真太郎, 冨士 岳志, 服部 佳功, 小関 健由, 小坂 健, 佐々木 啓一, 辻 一郎

    宮城県公衆衛生学会会誌　(45)　5-5　2013/03

    Publisher: 宮城県公衆衛生学会

    ISSN： 0912-747X

  52. 刺激唾液分泌量と口腔内健康指標との関連

    吉田英子, 細川亮一, 丹田奈緒子, 伊藤恵美, 鈴木淳, 玉原亨, 小島健, 田浦勝彦, 小関健由

    東北大学歯学雑誌　31　(2)　71-78　2012/12/28

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  53. 全身状態と口腔内因子の刺激唾液流出量との関連について

    吉田英子, 玉原亨, 細川亮一, 伊藤恵美, 丹田奈緒子, 斎藤恵一, 田浦勝彦, 小関健由

    口腔衛生学会雑誌　62　(2)　228-228　2012/04/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  54. 東北大学病院における病棟患者における口腔ケアの臨床的検討

    玉原亨, 細川亮一, 伊藤恵美, 吉田英子, 丹田奈緒子, 小関健由

    口腔衛生学会雑誌　62　(2)　213-213　2012/04/30

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  55. 「公衆衛生」、「地域保健」、「口腔保健」の定義 デルファイ法を用いた合意形成過程とその収束結果

    深井 穫博, 相田 潤, 安細 敏弘, 伊藤 博夫, 小川 祐司, 小関 健由, 佐々木 健, 白石 雅照, 千葉 逸朗, 鶴本 明久, 八重垣 健, 日本口腔衛生学会地域口腔保健委員会

    口腔衛生学会雑誌　62　(2)　206-206　2012/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  56. 東日本大震災による歯科医院への通院の中断と再開とその関連要因

    末永 竜右, 相田 潤, 竹内 研時, 若栗 真太郎, 服部 佳功, 小関 健由, 小坂 健, 佐々木 啓一, 辻 一郎

    口腔衛生学会雑誌　62　(2)　255-255　2012/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  57. 震災による義歯喪失の実態とその影響について

    佐藤 遊洋, 相田 潤, 竹内 研時, 若栗 真太郎, 服部 佳功, 小関 健由, 小坂 健, 佐々木 啓一, 辻 一郎

    口腔衛生学会雑誌　62　(2)　256-256　2012/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  58. Breath acetone in type 1 and type 2 diabetes mellitus

    Naoko Tanda, Yoshinori Hinokio, Jumpei Washio, Nobuhiro Takahashi, Takeyoshi Koseki

    Interface Oral Health Science 2011　212-214　2012/01/01

    DOI： 10.1007/978-4-431-54070-0_59 　

    More details Close

    ? Springer 2012. Difference of breath acetone between type 1 and type 2 diabetes mellitus (DM) is examined under informed consent. We measure acetone in mouth air and in deep breath by Gas Analyzer? XG-100V (New Cosmos Electric Co., Ltd., Osaka, Japan) and volatile sulfide compound in mouth air by Breathtron? (New Cosmos Electric Co., Ltd., Osaka, Japan), after meal. The acetone ratio of deep exhaled breath to mouth air and concentration of volatile sulfide compound (VSC) are com- pared between type 1 and type 2 DM patients. The acetone ratio of deep exhaled breath to mouth air is significantly higher in type 1 DM than type 2 DM patients. The concentration of VSC shows no significant difference. Though more cases should be studied, there is a possibility that the acetone ratio might reflect metabolic disorder of type 1 DM patients.

  59. 災害時の高齢者対策 長寿科学総合研究事業『災害時高齢者医療の初期対応と救急搬送基準に関するガイドライン作成』班成果発表講演会 大津波が残したもの 瓦礫の山と高齢者の疾患

    冲永 壯治, 大東 久佳, 小関 健由, 荒井 啓行

    日本集団災害医学会誌　16　(3)　379-379　2011/12

    Publisher: (一社)日本集団災害医学会

    ISSN： 1345-7047

  60. 山形県歯周疾患検診事業の受診者における口腔内状況と現状のその満足度について

    鈴木 欣一, 五十嵐 雄一, 阿部 友宏, 清水 一衛, 桜井 久夫, 齋藤 裕太, 佐藤 博嗣, 斎藤 純一, 石黒 慶一, 鍛治山 徹, 相田 潤, 田浦 勝彦, 山田 雄大, 鈴木 淳, 濱本 宜興, 小関 健由

    口腔衛生学会雑誌　60　(3)　243-243　2010/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  61. 2. 唾液を用いた口腔ケアの評価法の確立に関する研究(一般口演,第56回東北大学歯学会講演抄録)

    細川 亮一, 田浦 勝彦, 鈴木 浮, 伊藤 恵美, 小関 健由

    東北大学歯学雑誌　29　(1)　31-31　2010/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  62. 2008年東北六県における集団応用フッ化物洗口の実態調査報告

    田浦 勝彦, 相田 潤, 細川 亮一, 丹田 奈緒子, 井川 恭子, 齋藤 恵一, 鈴木 淳, 伊藤 恵美, 木本 一成, 小関 健由

    口腔衛生学会雑誌　60　(2)　179-179　2010/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  63. 宮城県美里町における介護予防口腔機能向上事業の展開

    相原浩子, 若生沙希, 横山太一, 鈴木正樹, 沼田いく子, 結城直子, 大井孝, 水戸祐子, 服部佳功, 坪井明人, 小関健由, 渡邉誠

    老年歯科医学　24　(2)　232-233　2009/09/30

    ISSN： 0914-3866

  64. フッ化物洗口の都道府県別にみた普及の推移 国の政策が果たした役割の検討

    田浦 勝彦, 相田 潤, 安藤 雄一, 晴佐久 悟, 田口 千恵子, 木本 一成, 葭原 明弘, 筒井 昭仁, 眞木 吉信, 荒川 浩久, 飯島 洋一, 磯崎 篤則, 小林 清吾, 小関 健由

    口腔衛生学会雑誌　59　(4)　516-516　2009/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  65. 成人検診における安静時唾液流出量と全身状態の関連

    鈴木 淳, 小川 裕平, 細川 亮一, 伊藤 恵美, 井川 恭子, 丹田 奈緒子, 齋藤 恵一, 田浦 勝彦, 相田 潤, 小関 健由

    口腔衛生学会雑誌　59　(4)　445-445　2009/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  66. 宮城県美里町における介護予防口腔機能向上事業の展開

    相原 浩子, 若生 沙希, 横山 太一, 鈴木 正樹, 沼田 いく子, 結城 直子, 大井 孝, 水戸 祐子, 服部 佳功, 坪井 明人, 小関 健由, 渡邉 誠

    日本老年歯科医学会総会・学術大会プログラム・抄録集　20回　183-183　2009/06

    Publisher: (一社)日本老年歯科医学会

  67. 地域歯科保健を推進する住民参加型学習歯科健診の取り組み

    小関健由, 小関健由, 斎藤恵一, 井川恭子, 細川亮一, 鈴木淳, 伊藤恵美, 近江浩昭, 虻江勝, 木村俊春, 庄司達雄, 野田清一, 山崎達見, 伏見行則, 白石信也, 曽根憲一, 高橋文一, 田浦勝彦, 丹田奈緒子, 佐々木早苗

    みちのく歯学会雑誌　40　(1-2)　2009

    ISSN： 0385-0099

  68. 東北大学大学院歯学研究科の地域歯科保健推進への取り組み 歯学研究科地域歯科保健推進室の果たす役割

    小関 健由, 小坂 健

    みちのく歯学会雑誌　39　(1-2)　30-30　2008/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  69. 良好な口腔のQOLは、定期的な歯科健診と関係する 5年間の山形県歯周疾患検診の結果から

    鈴木 欣一, 五十嵐 雄一, 阿部 友宏, 清水 一衛, 桜井 久夫, 齋藤 裕太, 鍛治山 徹, 相田 潤, 田浦 勝彦, 山田 雄大, 鈴木 淳, 濱本 宜興, 小関 健由, 佐藤 博嗣

    日本歯科医師会雑誌　61　(5)　553-553　2008/08

    Publisher: (公社)日本歯科医師会

    ISSN： 0047-1763

  70. 中日両国における歯周疾患の罹患状況と質問紙調査から導かれる予防策の検討

    小関 健由, 胡 徳瑜

    日中医学　23　(2)　52-52　2008/07/25

    ISSN： 0912-6287

  71. 歯科医師の社会貢献に関する意識調査について

    小関 健由, 猪狩 和子, 井川 恭子, 相田 潤, 小暮 由佳子, 大内 康弘, 山崎 尚哉

    口腔衛生学会雑誌　58　(3)　197-197　2008/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  72. 保育園児と幼稚園児の齲蝕罹患状況の相違について : 歯科健診標準化と健診結果集約システムにおける分析

    猪狩 和子, 小関 健由, 塚田 満男, 天野 三榮子, 小菅 玲, 福本 敏

    小児歯科学雑誌　46　(2)　253-253　2008/04/25

    ISSN： 0583-1199

  73. Guideline for Diagnosis of Dry Mouth in the Disabled

    KAKINOKI Yasuaki, MAKI Yoshinobu, OGASAWARA Tadashi, KOSEKI Takeyoshi, NISHIHARA Tatsuji, KIKUTANI Takeshi, UEDA Koichiro, WATANABE Shigeru, KISHIMOTO Etsuo

    (27)　30-34　2008/03/31

    ISSN： 0286-164X

  74. 通所介護施設のサービス利用者における基本チェックリスト口腔関連項目と歯科医療ニーズとの関連

    野口 有紀, 相田 潤, 丹田 奈緒子, 山田 雄大, 小川 裕平, 天野 一宇, 伊藤 恵美, 小関 健由, 小坂 健

    口腔衛生学会雑誌　57　(4)　378-378　2007/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  75. 名取市における乳幼児歯科保健事業の効果の検討

    小齋 薫, 相田 潤, 伊藤 恵子, 岡村 圭子, 渋谷 得江, 小坂 健, 小関 健由

    口腔衛生学会雑誌　57　(4)　406-406　2007/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  76. 通所介護施設での通所サービス利用者の歯科医療ニーズについて

    相田 潤, 野口 有紀, 丹田 奈緒子, 山田 雄大, 小川 裕平, 天野 一宇, 伊藤 恵美, 小関 健由, 小坂 健

    口腔衛生学会雑誌　57　(3)　225-225　2007/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  77. 名取市における3歳児のう蝕要因に関する調査結果の解析

    小齋 薫, 相田 潤, 伊藤 恵子, 岡村 圭子, 渋谷 得江, 小坂 健, 小関 健由

    口腔衛生学会雑誌　57　(3)　228-228　2007/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  78. 要介護高齢者の義歯装着・喫煙経験年数および薬剤の服用の関係について

    野口 有紀, 相田 潤, 丹田 奈緒子, 山田 雄大, 小川 裕平, 天野 一宇, 伊藤 恵美, 小関 健由, 小坂 健

    口腔衛生学会雑誌　57　(3)　229-229　2007/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  79. 東北地方の地域歯科保健推進へ向けた東北大学の取り組み 東北大学地域歯科保健推進室の設置

    小関 健由, 小坂 健, 高橋 信博, 佐々木 啓一, 高田 春比古, 菊地 正嘉, 川村 仁, 渡邉 誠, 東北大学大学院歯学研究科社会貢献委員会

    口腔衛生学会雑誌　57　(3)　229-229　2007/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  80. 5.新型定圧プローブによるプロービングの規格化への試み(第49回東北大学歯学会講演抄録,歯学情報)

    伊藤 恵美, 加藤 恵, 天野 一宇, 小川 裕平, 山田 雄大, 井川 恭子, 大木 亜紀子, 鍔原 泰介, 井川 資英, 島内 英俊, 小関 健由, 東北大学大学院歯学研究科口腔保健発育学講座予防歯科学分野, 山形歯科専門学校歯科衛生士科, 東北大学大学院歯学研究科口腔保健発育学講座予防歯科学分野, 東北大学大学院歯学研究科口腔保健発育学講座予防歯科学分野, 東北大学大学院歯学研究科口腔保健発育学講座予防歯科学分野, 東北大学大学院歯学研究科口腔保健発育学講座予防歯科学分野, 口腔生物学講座歯内歯周治療学分野, 口腔生物学講座歯内歯周治療学分野, 口腔生物学講座歯内歯周治療学分野, 口腔生物学講座歯内歯周治療学分野, 東北大学大学院歯学研究科口腔保健発育学講座予防歯科学分野

    東北大学歯学雑誌 = Tohoku University dental journal　25　(2)　88-88　2006/12/01

    ISSN： 0287-3915

  81. 東北大型定圧歯周プローブによるプロービングの規格化への試み

    井川 恭子, 伊藤 恵美, 青葉 達夫, 星野 秀明, 小関 健由

    口腔衛生学会雑誌 = JOURNAL OF DENTAL HEALTH　56　(4)　606-606　2006/08/30

    ISSN： 0023-2831

  82. A case report of chronic periodontitis patient treated 15 years

    ITO Emi, SAITO Jun-ichi, KOSEKI Takeyoshi

    48　211-211　2006/03/31

    ISSN： 0385-0110

  83. Stable Functionality of Tohoku University-type Power-Sensitive Periodontal Probe

    ITO Emi, IKAWA Kyoko, KATO Megumi, ISHII Eiichiro, AOBA Tatsuo, SHIMAUCHI Hidetoshi, KOSEKI Takeyoshi

    48　171-171　2006/03/31

    ISSN： 0385-0110

  84. 4. 口腔内環境計測装置の試作(一般口演,第47回東北大学歯学会講演抄録)

    石井 瑛一郎, 山田 雄大, 小川 裕平, 天野 一宇, 五十嵐 公英, 高橋 信博, 佐々木 啓一, 小関 健由, 東北大学大学院歯学研究科 予防歯科学分野, 東北大学大学院歯学研究科 予防歯科学分野, 東北大学大学院歯学研究科 予防歯科学分野, 東北大学大学院歯学研究科 予防歯科学分野, 東北大学大学院歯学研究科 口腔生化学分野, 東北大学大学院歯学研究科 口腔生化学分野, 東北大学大学院歯学研究科 口腔システム補綴学分野, 東北大学大学院歯学研究科 予防歯科学分野

    東北大学歯学雑誌 = Tohoku University dental journal　24　(2)　69-69　2005/12/27

    ISSN： 0287-3915

  85. Host-parasite interface and preventive dentistry

    Takeyoshi Koseki

    International Congress Series　1284　140-149　2005/09

    DOI： 10.1016/j.ics.2005.07.035 　

    ISSN： 0531-5131

  86. 2. 東北大学病院リハビリテーション科での口腔ケアの試み(第46回東北大学歯学会講演抄録)

    丹田 奈緒子, 松坂 朋典, 小関 健由, 東北大学病院予防歯科, 東北大学大学院歯学研究科口腔保健発育学講座予防歯科学分野, 東北大学大学院歯学研究科口腔保健発育学講座予防歯科学分野

    東北大学歯学雑誌 = Tohoku University dental journal　24　(1)　35-35　2005/06/30

    ISSN： 0287-3915

  87. 8. 口腔育成実習参加者における口腔状況年次推移(第45回東北大学歯学会講演抄録)

    井川 恭子, 小澤 雄樹, 高橋 一郎, 畑 真二, 小関 健由, 五十嵐 薫, 真柳 秀昭, 東北大学大学院歯学研究科 口腔保健学講座 予防歯科学分野, 東北大学大学院歯学研究科 口腔保健学講座 予防歯科学分野, 東北大学大学院歯学研究科 口腔保健学講座 顎口腔矯正学分野, 東北大学大学院歯学研究科 口腔保健学講座 小児発達歯科学分野, 東北大学大学院歯学研究科 口腔保健学講座 予防歯科学分野, 東北大学大学院歯学研究科 口腔保健学講座 口腔障害科学分野, 東北大学大学院歯学研究科 口腔保健学講座 小児発達歯科学分野

    東北大学歯学雑誌 = Tohoku University dental journal　23　(2)　90-90　2004/12/30

    ISSN： 0287-3915

  88. Mechanical stress induces the osteoblast differentiation and activates Runx2 and activator protein-1 through diverse MAPK signal transduction pathways

    T Kanno, T Tsujisawa, W Ariyoshi, T Koseki, T Takahashi, T Nishihara

    JOURNAL OF BONE AND MINERAL RESEARCH　19　S273-S273　2004/10

    ISSN： 0884-0431

  89. 幼児の食環境条件の乳歯う蝕に及ぼす影響

    熊谷 利花, 田浦 勝彦, 小関 健由

    口腔衛生学会雑誌 = JOURNAL OF DENTAL HEALTH　54　(4)　357-357　2004/08/30

    ISSN： 0023-2831

  90. 地域連携を重視した歯科保健への取り組み

    芳賀めぐみ, 成沢喜美子, 阿部陽子, 小橋純子, 岩倉政城, 小関健由

    口腔衛生学会雑誌　54　(5)　2004

    ISSN： 0023-2831

  91. Microbial etiology of periodontitis

    T Nishihara, T Koseki

    PERIODONTOLOGY 2000　36　14-26　2004

    DOI： 10.1111/j.1600-0757.2004.03671.x 　

    ISSN： 0906-6713

  92. 歯周病をどう予防するか 「かかりつけ歯科医」の役割について (東北大学歯学雑誌)

    小関健由

    東北大学歯学雑誌　22　(2)　67-78　2003/12

  93. レーザー光を用いた歯の表面粗さ計の基礎的研究

    小関 健由, 井川 恭子, 栗原 えりこ, 安細 敏弘, 竹原 直道

    口腔衛生学会雑誌　53　(4)　469-469　2003/08/30

    ISSN： 0023-2831

  94. 仙台市内保育所幼児のう蝕有病状況の推移について

    田浦 勝彦, 井川 恭子, 齋藤 恵一, 小関 健由

    口腔衛生学会雑誌　53　(4)　497-497　2003/08/30

    ISSN： 0023-2831

  95. レーザー光を利用した歯肉縁下歯石検出の試み

    栗原 えりこ, 小関 健由, 郷原 賢次郎, 西原 達次, 安細 敏弘, 竹原 直道

    口腔衛生学会雑誌　53　(4)　337-337　2003/08/30

    ISSN： 0023-2831

  96. 5.歯垢中のmutans streptococciのPCR法による検出について(一般演題)(第42国東北大学歯学会講演抄録)

    熊谷 崇, 重光 竜二, 太郎丸 毅, 鷲尾 純平, 小関 健由, 佐藤 拓一, 高橋 信博

    東北大学歯学雑誌 = Tohoku University dental journal　22　(1)　54-54　2003/06/30

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  97. Low-molecular-weight hyaluronic acid activates osteoclastic differentiation and function.

    W. Ariyoshi, T. Kanno, H. Ichimiya, H. Takano, T. Koseki, T. Takahashi, T. Nishihara

    JOURNAL OF DENTAL RESEARCH　82　B393-B393　2003/06

    ISSN： 0022-0345

  98. Bone-regenerative profiles of bone growth factors and matrix protein expression in mechanically strained human osteoblast-like cells examined by RT-PCR.

    T. Kanno, T. Tsujisawa, W. Ariyoshi, M. Iwamura, T. Koseki, T. Takahashi, T. Nishihara

    JOURNAL OF DENTAL RESEARCH　82　B381-B381　2003/06

    ISSN： 0022-0345

    eISSN： 1544-0591

  99. 唾液と臨床症状 (2) 歯周病と唾液

    小関健由

    唾液と口腔乾燥症　20-21　2003

    Publisher: 医歯薬出版

  100. 唾液の曳糸性測定方法及び測定値の粘度との関連について

    郷原 賢次郎, 小関 健由, 安細 敏弘, 石川 正夫, 渋谷 耕司, 西原 達次

    歯科基礎医学会雑誌　44　(5)　443-443　2002/09/20

    Publisher: 歯科基礎医学会

    ISSN： 0385-0137

  101. Actinobacillus actinomycetemcomitans由来のCytolethal Distending Toxinによる細胞障害の機序解析

    三瀬 功二, 秋房 住郎, 小関 健由, 安細 敏弘, 西原 達次

    歯科基礎医学会雑誌　44　(5)　384-384　2002/09/20

    Publisher: 歯科基礎医学会

    ISSN： 0385-0137

  102. P-13. Examination of surroundings to apply "NEVA METER" for practical use

    Gohara Kenjiro, Koseki Takeyoshi, Awano Shuji, Ansai Toshihiro, Takehara Tadamichi, Nishihara Tatsuji

    The Journal of the Kyushu Dental Society　56　(4)　183-184　2002/08/25

    Publisher: Kyushu Dental Society

    ISSN： 0368-6833

  103. 13. Effects of PPR (Platelet Rich Plasma) on human osteoblast like cells

    Kanno Takahiro, Koseki Takeyoshi, Tsujisawa Toshiyuki, Ariyoshi Wataru, Takano Hiroshi, Funaki Katsuyuki, Takahashi Tetsu, Nishihara Tatsuji

    The Journal of the Kyushu Dental Society　56　(4)　172-172　2002/08/25

    Publisher: Kyushu Dental Society

    ISSN： 0368-6833

  104. 14. Inhibitory effect of glycosaminoglycan on the formation of osteoclasts

    Ariyoshi Wataru, Koseki Takeyoshi, Kanno Takahiro, Takano Hiroshi, Nishihara Tatsuji, Takahashi Tetsu

    The Journal of the Kyushu Dental Society　56　(4)　172-173　2002/08/25

    Publisher: Kyushu Dental Society

    ISSN： 0368-6833

  105. 17. Expression of endothelin-1 in gingival epithelial cells

    Yamamoto Eri, Awano Shuji, Koseki Takeyoshi, Ansai Toshihiro, Takehara Tadamichi

    The Journal of the Kyushu Dental Society　56　(4)　174-174　2002/08/25

    Publisher: Kyushu Dental Society

    ISSN： 0368-6833

  106. 曳糸性測定器ネバ・メーターの唾液曳糸性測定に関わる条件の検討 : 新しく開発された唾液物性測定器の使用法について

    郷原 賢次郎, 小関 健由, 柿木 保明, 越宗 紳二郎, 栗野 秀慈, 安細 敏弘, 竹原 直道

    口腔衛生学会雑誌　52　(4)　582-583　2002/08/15

    ISSN： 0023-2831

  107. 17 歯肉上皮細胞における endothelin-1 の発現

    山本 英理, 粟野 秀慈, 小関 健由, 安細 敏弘, 竹原 直道

    九州歯科学会総会抄録プログラム　62　15-15　2002/05/25

    Publisher: 九州歯科学会

  108. 14 グリコサミノグリカンによる破骨細胞誘導系の抑制作用についての研究

    有吉 渉, 小関 健由, 管野 貴浩, 高野 裕史, 西原 達次, 高橋 哲

    九州歯科学会総会抄録プログラム　62　14-14　2002/05/25

    Publisher: 九州歯科学会

  109. 13 骨芽細胞に対する PRP (Platelet Rich Plasma) の効果について

    管野 貴浩, 小関 健由, 辻澤 利行, 有吉 渉, 高野 裕史, 船木 勝介, 高橋 哲, 西原 達次

    九州歯科学会総会抄録プログラム　62　13-13　2002/05/25

    Publisher: 九州歯科学会

  110. P-13 唾液曳糸性測定器ネバメーターの測定条件の考察

    郷原 賢次郎, 小関 健由, 越宗 紳二郎, 粟野 秀慈, 安細 敏弘, 竹原 直道, 西原 達次

    九州歯科学会総会抄録プログラム　62　24-24　2002/05/25

    Publisher: 九州歯科学会

  111. RGF-b1 and activin A promote osteoclastogenesis induced by RANKL

    T Koseki, T Ansai, T Nishihara, T Takehara

    JOURNAL OF DENTAL RESEARCH　81　A106-A106　2002/03

    ISSN： 0022-0345

  112. Levels of Interleukin-8 and Nitrite in the Saliva of Patients with Periodontitis

    AWANO Shuji, ANSAI Toshihiro, KUSABA Akito, KOSHIMUNE Shinjiro, YAMAMOTO Eri, KOSEKI Takeyoshi, TAKEHARA Tadamichi

    38　89-91　2002/03/01

    ISSN： 0070-3737

  113. Regulatory Effects of Inflammatory Factors on CD14 Expression

    MOCHIZUKI Satoru, KOBAYASHI Makoto, NISHIHARA Tatsuji, KOSEKI Takeyoshi, OIKAWA Akiko, HASEGAWA Kohji

    Journal of the Japanese Association of Periodontology　43　125-125　2001/09/30

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  114. Actinobacillus actinomycetemcomitans由来cytolethal distending toxinによるマウスB細胞のp53非依存性p21CIP1/WAF1発現と細胞周期G2/M期停止の誘導

    佐藤 毅, 小関 健由, 大和 建嗣, 西原 達次, 石川 烈

    歯科基礎医学会雑誌　43　(5)　535-535　2001/08/20

    Publisher: 歯科基礎医学会

    ISSN： 0385-0137

  115. Mechanisms of the cell cycle arrest induced by recombinant cytolethal distending toxin of Actinobacillus actinomycetemcomitans

    Sato Tsuyoshi, Koseki Takeyoshi, Nishihara Tatsuji, Ishikawa Isao

    Journal of the Japanese Association of Periodontology　42　61-61　2000/04/15

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  116. A novel of Actinobacillus actinomycetemcomitans induces apoptosis of HL-60 cells.

    T He, T Kishihara, T Sato, T Koseki, S Oda, Ishikawa, I

    JOURNAL OF DENTAL RESEARCH　79　231-231　2000

    ISSN： 0022-0345

  117. 歯周病とう蝕

    小関 健由, 花田 信弘

    Current Concepts in Infectious Diseases　19　(2)　18-19　2000/01

    Publisher: (株)ティ・エル・エム・ジャパン

    ISSN： 0289-775X

  118. Modulation of apoptosis in Actinobacillus actinomycetemcomitans-infected macrophages by protein kinase

    NONAKA Koji, MURO Miyuki, KATO Satsuki, KOSEKI Takeyoshi, NISHIHARA Tatsuji, KOWASHI Yusuke

    Journal of the Japanese Association of Periodontology　39　138-138　1997/03/25

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  119. Genotyping of Actinobacillus actinomycetemcomitans by AP-PCR

    HE Tao, HAYASHI Joichiro, YAMAMOTO Matsuo, WATANABE Hisashi, KOSEKI Takeyoshi, NISHIHARA Tatsuji, ISHIKAWA Isao

    Journal of the Japanese Association of Periodontology　38　97-97　1996/09/05

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  120. Apoptosis in activated macrophages induced by Actinobacillus actinomycetemcomitans infection

    MURO Miyuki, KOSEKI Takeyoshi, KATO Satsuki, NISHIHARA Tatsuji, KOWASHI Yusuke

    Journal of the Japanese Association of Periodontology　38　99-99　1996/09/05

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  121. Establishment of baculovirus expression system of recombinant Actinobacillus

    Koseki Takeyoshi, Zhang-Koseki Yi Jie, Hanada Nobuhiro, Nishihara Tatsuji

    Journal of the Japanese Association of Periodontology　38　(秋季特別号)　160-160　1996/09/05

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  122. Regulation of Interleukin-1 Production in Human Monocytic Cells by Activin

    Ohguchi Masahiro, Koide Masanori, Koseki Takeyoshi, Ueda Nobuo, Ishihara Yuichi, Amano Katuhiko, Nishihara Tatsuji, Noguchi Toshihide

    Journal of the Japanese Association of Periodontology　38　(秋季特別号)　114-114　1996/09/05

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  123. Apoptosis and Growth Arrest Induced by Activins in B Cell Hybridomas

    KOSEKI Takeyoshi, YAMATO Kenji, NAKAMURA Takanori, SUGINO Kishiko, SUGINO Hiromu, NISHIHARA Tatsuji

    19　846-846　1996/08/01

  124. Analysis of mechanism of Apoptotic cell death of macrophages induced by Actinobacillus actinomycetemcomitans

    MURO Miyuki, KOSEKI Takeyoshi, KATO Satsuki, NISHIHARA Tatsuji, KOWASHI Yusuke

    Journal of the Japanese Association of Periodontology　38　96-96　1996/03/25

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  125. The genotype distribution of Actinobacillus actinomycetemcomitans by AP-PCR

    T He, J Hayashi, T Koseki, M Umeda, H Watanabe, Ishikawa, I

    JOURNAL OF DENTAL RESEARCH　75　1982-1982　1996

    ISSN： 0022-0345

  126. BCL-2 confers resistance to activin A-induced apoptosis on B cell hybridomas

    T Koseki, T Nishihara

    JOURNAL OF DENTAL RESEARCH　75　2377-2377　1996

    ISSN： 0022-0345

  127. Mechanism of induction of Apopotosis in Actinobacillus actinomycetemcomitans infected macrophages

    MURO Miyuki, KATO Satsuki, KOSEKI Takeyoshi, OIDO Mari, NISHIHARA Tatsuji, KOWASHI Yusuke

    Journal of the Japanese Association of Periodontology　37　131-131　1995/09/06

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  128. Purification of Serotype d- and e- specific Antigens from Actinobacillus actinomycetemcomitans and Seroclassification of Clinical Isolates from Periodontal Patients

    YAMAMOTO Matsuo, KOSEKI Takeyoshi, NISHIHARA Tatsuji, WATANABE Hisashi, ISHIKAWA Isao

    Journal of the Japanese Association of Periodontology　37　137-137　1995/09/06

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  129. Long term observations of the patients with periodontal dieases

    BANDOH Kaoru, KOSEKI Takeyoshi, SUGIYAMA Eiichi, ISHIKAWA Isao

    Journal of the Japanese Association of Periodontology　36　130-130　1994/09/10

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  130. Inhibitory effect of <Porphyromonas>___-<gingivalis>___- on platelet-activating factor production in human neutrophils

    NOGUCHI Kazuyuki, YANAI Masayuki, KOSEKI Takeyoshi, ISHIKAWA Isao

    Journal of the Japanese Association of Periodontology　36　89-89　1994/03/25

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  131. Inhibitory effect of <P.gingivalis>___- on 5-HETE and LTB_4 production of human PMNLs

    YANAI Masayuki, KOSEKI Takeyoshi, NOGUCHI Kazuyuki, ISHIKAWA Isao

    Journal of the Japanese Association of Periodontology　34　128-128　1992/09/16

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  132. Characterization of Oral Treponemes

    Koseki Takeyoshi, Umeda Makoto, Benno Yoshimi, Ishikawa Isao

    Journal of the Japanese Association of Periodontology　34　(0)　100-100　1992/04/20

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  133. The investigation and treatment of two Papillon-Lefevre syndrome patients

    UMEDA Makoto, NISHI Nobumasa, KOSEKI Takenori, LAOSRISIN Narongsak, ZHANG Yijie, ISHIKAWA Isao

    Journal of the Japanese Association of Periodontology　31　71-71　1989/09/22

    Publisher: The Japanese Society of Periodontology

    ISSN： 0385-0110

  134. THE INVESTIGATION OF 2 PAPILLON-LEFEVRE SYNDROME PATIENTS

    M UMEDA, N NISHI, T KOSEKI, N LAOSRISIN, ISHIKAWA, I

    JOURNAL OF DENTAL RESEARCH　68　(4)　674-674　1989/04

    ISSN： 0022-0345

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Industrial Property Rights 6

  1. 歯間フロス用糸

    柴田, 恵, 美子, 小関 健由

    Property Type: Patent

  2. 歯間フロス用糸

    柴田, 恵, 美子, 小関 健由

    特許第5992700号

    Property Type: Patent

  3. 歯周病検査用プローブ

    小関 健由, 石田 和也

    Property Type: Patent

  4. 歯周病検査用プローブ

    小関 健由, 石田 和也

    特許第4425199号

    Property Type: Patent

  5. 口腔内設置型生体モニター・治療装置

    西原 達次, 小関 健由

    Property Type: Patent

  6. 口腔内設置型生体モニター・治療装置

    西原 達次, 小関 健由

    特許第4264472号

    Property Type: Patent

Show all Show first 5

Research Projects 46

  1. プロービングの規格化に向けて Competitive

    2005/04 - Present

  2. 唾液性状と機能に関する予防歯学的研究 Competitive

    2005/04 - Present

  3. 根面齲蝕の評価法の研究 Competitive

    2004/04 - Present

  4. 化学療法誘発性末梢神経障害の実態解明およびリスク・増悪因子の検索

    石河 理紗, 瀬名 浩太郎, 小関 健由

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2023/04/01 - 2026/03/31

  5. 呼気オミックスを用いた乳がん口腔支援システムの開発

    丹田 奈緒子, 石田 孝宣, 赤池 孝章, 鷲尾 純平, 多田 寛, 高橋 信博, 小関 健由

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

  6. Creation of a dental skill distance education system incorporating a clinical skill AI evaluation engine

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2022/04/01 - 2025/03/31

  7. Development of risk assessment system of dental caries based upon hardness of hard tissue and remineralization method of enamel surfaces

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2020/04/01 - 2023/03/31

  8. 標的指向性マイクロバブルと金属ナノ粒子及び低出力超音波を用いた口腔癌治療法の確立

    岩永 賢二郎, 丹田 奈緒子, 小関 健由

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 九州歯科大学

    2020/04/01 - 2023/03/31

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    抗体修飾マイクロバブルと超音波の併用による抗癌剤導入法の有効性の解析：抗体修飾マイクロバブルの作製は、既にプロトコール化されている。同法によるブレオマイシンの導入後、培養48時間後にWST-8による生細胞数の評価を行ったところ、BLM単独群やマイクロバブルを使用したBLM導入群、またIgG修飾マイクロバブルを使用したBLM導入群では、そこまで細胞数の減少は認められなかったが、抗EGFR抗体修飾マイクロバブルを併用したBLM導入群では有意に生細胞数が減少した。 また，一般的な2次元培養下の細胞は組織環境が生体内と大きく乖離している。口腔癌細胞においても薬剤に対する感受性や増殖様式は生体内の挙動が異なると考えられる。細胞同士が凝集した球状の集合体（スフェロイド）を大量に作製可能なデバイスを用いて、口腔癌組織の環境を模倣した3次元培養を行い、同法による癌治療の開発に生かせないか検討した。HSC-3とCa9-22をそれぞれ培養し、非接着処理を施したマイクロウェルチップ上に播種した。形成されたスフェロイドの形態観察と直径の計測およびPCRによる遺伝子発現の解析を行った。デバイス上に播種したHSC-3とCa9-22は、培養5日目にかけて徐々に凝集し、直径も短縮化した。また、幹細胞マーカーのCD44・Oct4・Nanog・Sox2と低酸素性マーカーVEGFの発現量は、スフェロイド群では2次元培養群と比較していずれも亢進していることが確認された。LIVE/DEAD染色では培養5日目においても、スフェロイド内部は生細胞を主体に構成されていることが示された。回収したスフェロイドは2次元培養下で定着・増殖し、幹細胞マーカーの発現は維持されていた。専用デバイスを用いた口腔癌スフェロイドは生態環境に近い培養法であることが示唆された。

  9. Development of a comprehensive reconstruction of the oral environment by detailed physicochemical and functional analysis of saliva

    KOSEKI Yakeyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

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    In the physicochemical function analysis of saliva, cross-sectional analysis of stimulated saliva collected at the time of adult oral health examination and component analysis of resting saliva with different viscosity in the same person were performed. In salivary components, various glycoproteins play an important role in determining the oral environment. Our study indicated that several salivary proteins in the high and low molecular weight might be associated with general health condition in stimulated saliva. In addition, in a longitudinal survey of saliva during radiotherapy of head and neck cancer, changes in the physical properties of saliva and the concentrations of polysaccharides and glycoproteins were observed. From the obtained results, we tried to prepare artificial saliva that approximated the physical and biochemical properties of saliva, and considered the factors necessary for the reconstruction of the oral environment.

  10. Development of predictive oral supportive system for perioperative breast cancer patients

    Tanda Naoko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2018/04/01 - 2022/03/31

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    Bone is the most frequent metastatic site of breast cancer, and metastases cause a poor quality of life due to bone pain, pathological fracture, hypercalcemia, and spinal cord compression. Bone metastases in breast cancer patients are a common concern for medical doctors and dentists. Bone-modifying agents, which are necessary to prevent skeletal-related events, are associated with osteonecrosis of the jaw as an adverse side effect. Prognostic risk factors of bone metastases are important for predictive oral supportive system for breast cancer patients. Univariate analysis and multivariate analysis were performed using the Cox proportional hazards model to investigate associations between prognostic variables and time to bone metastasis in primary operable breast cancer patients. In multivariate analysis, tumor stage and alcohol sensitivity were significant prognostic risk factors for bone metastases.

  11. Exhaustive survey of development of oral function and health promotion factors on a large-scale birth cohort

    Koseki Takeyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    About two-thousand participates of large-scale birth cohort survey were analyzed about the health-related factors, such as environmental, hereditary, social and lifestyle factors. In the same time, we analyzed the behavior and the attitude of parents for oral health care and promotion. Furthermore, we developed the new method to estimate the risk of dental caries of deciduous tooth, which was suitable for mass examination. From these results, we established the sustainable research base and fundamental data of long-term cohort survey continuing more than ten years.

  12. The comprehensive survey for the risk factor of oral mucositis under the radiotherapy.

    Hosokawa Ryoichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    It is very important to prevent or reduce adverse events caused by the radiotherapy and chemotherapy for completing the treatment plan. Especially, the adverse events of head and neck cancer caused by radiotherapy are very severe. For instance, patients complain dry mouth and oral mucositis so on. Oral mucositis cause severe pain and reduce patient’s quality of life. It is very hard for patients to eat or talk due to the pain caused by oral mucositis. So it is requested to establish the methods inhibit oral mucositis during radiotherapy. In this study, we proposed the possibility to reduce the severity of oral mucositis using Hangeshasinto, which is one of traditional medicine in Japan in clinical study. Also we provide the mechanism how Hangeshasinto prevent oral mucositis by using cell culture experiments.

  13. Comprehensive analysis in oral microbiome as a promoting factor for esophageal cancer

    TANDA NAOKO, ITO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    Acetaldehyde is a powerful carcinogen in thoracic esophageal cancer. The aim of this study is to evaluate perioperative change of mouth acetaldehyde and its causal oral bacteria in thoracic esophageal cancer patients under intensive oral care. Analysis was done compared to control cases. Drinking and smoking histories were longer and oral condition was worse in patients group than in control. Acetaldehyde in mouth air of thoracic esophageal cancer patients decreased after oral intensive care before operation. It also decreased after operation. It is suggested that intensive oral care for perioperative thoracic esophageal cancer patients decreases acetaldehyde produced in oral microbiome.

  14. Guideline for the early diagnosis of residual ridge by using NaF-PET/CT and FEA

    SUENAGA HANAKO, YAMAGUCHI KEIICHIRO, Li Qing, Swain Mike

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    NaF Positron Emission Tomography (PET) can identify subtle functional variation prior to the major structural change detectable by X-ray. NaF-PET/CT scan was performed to examine the change of bone metabolism in the residual ridge underneath denture before and after the RPD treatment. The resultant mechanobiological response was analyzed using a 3D finite element (FE) model derived from the computerized tomography (CT) images of a corresponding patient under the occlusal loading. Mechanobiological stimulus determined from the FEA are quantified and compared with the standardized uptake values (SUV) of PET. The increased application of occlusal force after RPD insertion induced higher mechanical stimuli in the residual bone, and SUV increased in the region of residual ridge with higher mechanical stimuli. SUV had clear correlation with mechanical stimulus in the cancellous bone, especially after RPD insertion (R2 > 0.8, p < 0.01).

  15. Development of new evaluation system of mucosal inflammation based upon vascular imaging and preventive method of oral mucositis with oral mucosa mapping system

    KOSEKI Takeyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    To prevent the oral mucosal problem and to enhance the promotion of oral-related QOL, a preventive method of oral mucositis and mucosal injure was built for the elderly suffering xerostomia and for the patients who were undergoing perioperative medical treatments. The updated technology of visual examination of mucosa, so-called Narrow Band Imaging, was employed in this system to visualize the abnormality of oral mucosa. The oral mucosa mapping system which covered whole oral cavity was also developed. By using these two systems, new diagnostic method of oral mucosa was proposed to prevent oral mucositis. Finally, the comprehensive guideline of diagnosis and treatment of oral mucositis were discussed.

  16. Metabolome analysis of oral biofilm: a possible risk assessment of oral diseases using oral microbiome metabolism

    TAKAHASHI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    This study aimed to clarify the whole picture of metabolic systems of oral biofilm through the metabolome analysis and metabolic activity measurement, and to consider an assessment of oral health and disease using oral microbiome metabolism. The CE-MS metabolome system enabled to analyze the metabolites associated with carbohydrates and amino acids comprehensively and revealed the whole picture of metabolic systems of carbohydrates and amino acids in the oral biofilm. In addition, the metabolic activity of a tiny amount of oral biofilm was successfully measured using the alamarblue system. Furthermore, the metabolic activity was diverse among sample sites and metabolic substrates, suggesting that these metabolic characteristics of oral microbiome can be used as an indicator of oral health and disease.

  17. ナノ・マイクロバブルと超音波を用いた歯質へのフッ素導入法の開発

    菅崎 将樹, 小関 健由, 細川 亮一, 丹田 奈緒子, 末永 華子, 佐久間 陽子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2015/04/01 - 2016/03/31

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    口腔・頭頚部がんに対する放射線治療では、粘膜や皮膚の炎症、唾液分泌障害、嚥下障害、味覚障害など様々な副作用を生じ、一旦出現すると放射線療法中は悪化の一途をたどる。特に唾液腺は放射線感受性が高い臓器あり、照射線量が多くなるに従って唾液分泌量が低下し、40Gy 以上の線量では唾液腺が不可逆に障害され、照射終了後も重度の口腔乾燥を生じるといわれている。重度の口腔乾燥により唾液による自浄作用を失った口腔内では、歯牙自体が乾燥により脆くなることも相まって齲蝕が多発する。がんに対する放射線治療を受けた患者に対するフッ化物応用を含めた齲蝕予防法は確立しておらず、口腔乾燥に対しては保湿剤を使用し、スポンジブラシ等も併用して試行錯誤しながらできる限り口腔清掃を試みているのが現状である。一方、歯質強化にはフッ化物応用が有効であると考えられるが、重度の口腔乾燥では従来のフッ素の応用法では齲蝕予防のコントロールが難しく、より積極的な用法が必要と思われた。 本研究は、ナノ・マイクロバブルと超音波を用いた歯質へのフッ素導入法の開発を目的としている。平成27年度においては、ヒト抜去歯を用いるため東北大学大学院歯学研究科研究倫理委員会に倫理申請書を提出した。倫理委員会からの承認を得る間、作製予定のフッ化ナトリウム封入ナノ・マイクロバブルと同じナノ・マイクロバブルに蛍光色素を封入し、蛍光色素封入ナノ・マイクロバブルを作製、同ナノ・マイクロバブルの生体内での物理学的性状を検討し、フッ化ナトリウム封入ナノ・マイクロバブルの作製法の基礎的技術を確立した。

  18. Establishment of new research platform of functional salivary proteins with comprehensive proteasome analysis

    IIJIMA Wakana, HOSOKAWA Ryoichi, TANDA Naoko, KOSEKI Takeyoshi, SUGAZAKI Masaki, SAKUMA Yoko, SUENAGA Hanako, ITO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2014/04/01 - 2016/03/31

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    New research approach for salivary proteins by using the comprehensive proteasome analysis of functional salivary proteins, with the pooled saliva samples as standard markers, had established. This simple and comprehensive method was effective to research the relationship between the parameter of oral quality of life and each component of salivary proteins. New research platform of functional salivary proteins was proposed with comprehensive proteasome analysis from the viewpoint of healthy salivary function.

  19. Proposal of the guideline for the treatment and rehabilitation of the neck and head cancer based on the healing processes of soft tissues

    Yoshida Eiko, KOSEKI Takeyoshi, HOSOKAWA Ryoichi, KOYAMA Shigeto, TANDA Naoko, ITO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

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    To evaluate the recovery level of the craniofacial and oral functions after the treatment of the head and neck cancer, new estimation approach for healing of soft tissues, which had created by the surgical operation, had been studied. The index of oral functional rehabilitation was composed with the measurement values of oral functions and surgical wound healing. The patients who suffered the head and neck cancer were evaluated and compared the processes of oral functional recovery by using this evaluation method, with the observation of scar contraction and the oral functional rehabilitation, such as exercises of jaw opening.

  20. Establishment of the concept of rehabilitation with the oral caring with the effect of mental healing and recovery

    Ito Emi, KOSEKI Takeyoshi, HOSOKAWA Ryoichi, TANDA Naoko, SAKAI Nobuyuki, ARI Toshiyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

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    The concept of the oral caring had been established as an actual practice of mental healing and recovery by the intervention of oral cavity. The process of the oral caring was composed with the understanding of oral conditions with the method of organic and functional oral assessments, performing oral care and management, catching the mental reaction caused by the oral intervention, and providing advanced state of relaxation by the feedback. The oral caring was provided across all age groups with a sense of satisfaction and mental rehabilitation. The rehabilitation with the oral caring will consist of the concept of the oral caring with the effect of mental healing and recovery.

  21. Correlation between periodontal bacteria and progression of aortic aneurysm

    SAIKI Yoshikatsu, Kawamoto Shunsuke, Motoyoshi Naotaka, Hosokawa Ryoichi, Koseki Takeyoshi, Hosoyama Katsuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2013/04/01 - 2016/03/31

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    This study was designed to elucidate the effect of periodontal bacteria on the progression of aortic aneurysm by inspecting the specimens of aortic aneurysm surgery. So far, several aortic samples were examined of long-term culturing, followed by qPCR of 16sRNA for the identification of the bacterium. As a result, Staphylococcus spp. and Propioni bacterium acnes were detected, although periodontal bacteria were not detected.

  22. Development of artificial saliva-secreting automated device placed inside the mouth activated by oral environment and functional activities

    KOSEKI Takeyoshi, HOSOKAWA Ryoichi, TANDA Naoko, YOSHIDA Eiko, ITO Emi, NISHIHARA Tatsuji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04/01 - 2015/03/31

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    Saliva has important roles in maintaining the environment of the healthy mouth and in supporting the smooth activities of many oral functions in daily lives. When the secretion of saliva was reduced, the quality of life (QOL) related to the mouth would be heavily decreased, because of the increasing the risks of oral diseases and the dysfunctions of nutrition intake and difficulty in speaking. In this study, we proposed the means of maintaining good oral QOL by replacement therapy of artificial saliva in the patients with xerostomia, through analyzing the functions of saliva, preparing of artificial saliva, and developing the saliva-secreting automated device placed inside the mouth.

  23. Development and proposal of evaluation method of oral mucosal damages based on the degree of injuries and atrophy of oral mucosa and saliva properties

    KOSEKI Takeyoshi, TANDA Naoko, HOSOKAWA Ryoichi, SUZUKI Osamu, ITOU Emi, TAURA Katuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2011/04/01 - 2014/03/31

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    Novel diagnostic method and preventive treatment system of oral mucosa had been developed based upon the evaluation of the degree of injury and atrophy, to prevent oral problems of the elderly and side effects of cancer treatment. For this purpose, we established the haptic diagnostic devices of oral mucosa with the technology of ultrasound sensing. Using this device and conventional evaluation systems of oral environment, we analyzed the symptoms of injured oral mucosa, and objective estimation of damage on oral mucosa had advanced to utilize for the clinical applications. Based upon these results, total management plans to maintain the health of oral mucosa were discussed.

  24. Metabolomic approach to oral biofilm and saliva: possible risk indicator for oral diseases

    TAKAHASHI Nobuhiro, KOSEKI Takeyoshi, SENPUKU Hidenobu, SAKAMOATO Mitsuo, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2010/04/01 - 2014/03/31

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    Metabolome analysis for oral biofilm and saliva using CE-TOFMS was established. This method made it possible to analyze not only sugar metabolism but also amino acid metabolism in oral biofilm and even in saliva, where only small amount of metabolites (about 1/100 of oral biofilm) was contained. In addition, the analyses of the effect of sugar alcohols and fluorides on the biofilm metabolism suggested close coordination between sugar and amino acid metabolisms. Metabolome profiles of sugar and amino acid metabolisms can be used for risk indicators of dental caries and periodontitis/oral malodor, respectively.

  25. Parameters of oral environment for perioperative patients of lung cancer based on two dimensional analysis of breath gasses

    TANDA Naoko, HOSHIKAWA Yasushi, TAKAHSI Nobuhiro, KOSEKI Takeyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2011 - 2013

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    We analyzed gasses from exhaled and mouth air and saliva of perioperative patients of lung cancer at bedside before oral care, after oral care before operation, and after operation. Protective reflexes (cough and swallowing reflex) were also measured before and after operation. As for patients without postoperative pneumonia, oral care before operation increased ratio of salivary streptococci, usually known as healthy-associated bacteria, in anaerobic condition. Oral care also decreased total number of bacteria in saliva and decreased malodorous gasses. Acetone concentration increased after operation with rapid increase of CRP. This study suggests possibility that non-invasive measuring volatile sulfide compounds and volatile organic compounds at bedside indicate oral environments and general inflammatory conditions of perioperative lung cancer.

  26. Development of the debridement method of soft and hard tissues with novel functional strings

    ITO Emi, KOSEKI Takeyoshi, TAURA Katsuhiko, HOSOKAWA Ryoichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

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    The evaluation method of the effectiveness of biofilm removal was innovated and fundamental bases of researches and applications of novel functional strings were developed. Clinical application of novel functional strings, such as dental floss or swab sticks, revealed the high performance of biofilm removal and high biological compatibility with human oral mucosa. These results indicated the possibility of wide ranges of clinical application of novel functional strings, not only for oral hygiene but also for general medical use. The debridement method by using novel functional strings might be one of the best cleaning methods for every surface of human bodies, including soft and hard tissues.

  27. Clinical application of assessment devices for the risk of dental caries with ultrasonic haptic sensor technology

    KOSEKI Takeyoshi, HOSOKAWA Ryouichi, ITO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

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    Clinical diagnosis of initial regions of dental caries is important for oral health promotion and prevention of dental caries. To development of diagnostic devices of tooth surfaces condition, including the initial caries regions, we utilized the ultrasonic mineralization measurement system of hard tissues with ultrasonic technology of haptic sensor. In the clinical use of the ultrasonic mineralization measurement system, the thermal protective equipment was needed for the stabilization of readout, which was sensitively influent to the thermal shift of the sensing oscillator. Furthermore, for the data management of mass oral health check-up, the dental chart input method system was developed. Thus, the evaluation system of caries risk assessment was established.

  28. Study about the current state of environmental pollution and prevention control caused by formaldehyde mixture at dental clinics

    SHINDOH Taku, IKAWA Motohide

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2009 - 2011

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    According to the modification of the Industrial Safety and Health Law and Ordinance on Prevention of Hazards due to Specified Chemical Substances , the administrative level of the formaldehyde vapor concentration in working space was changed from 0. 25 ppm to 0. 1 ppm on administrative action to the evaluation of the risk of occupational diseases. In endodontic treatment, formaldehyde is commonly used for the intracanal dressing as a compound. The authors measured the formaldehyde vapor concentration in the nearby air of the simulated intracanal dressing of Formalin Guaiacol (FG). The results indicated that the root canal dressing of FG produced the formaldehyde vapor at the acceptable concentration designated by the law. There was a difference between the vapor concentration of the air collected in the summer and that in the winter. The use of dental vacuum was advocated during the root canal dressing of FG in order to decrease the vapor concentration .

  29. Development of diagnostic system of gingivitis based upon the objective index of peripheral vessel of inflamed tissues

    TAURA Katsuhiko, KOSEKI Takeyoshi, TAKAHASHI Nobuhiro, IKAWA Kyouko, TANDA Naoko, KOSEKI Ichie, SAITO Keiichi, HOSOKAWA Ryouichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2008 - 2010

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    To distinguish the inflamed gingivae and the healthy gingivae objectively and reproductively, we analyzed the bleeding and rubor, which are known as the clinical symptoms of inflamed tissues. From the researches of the estimation of rubor of inflamed gingival tissues, it might be not useful indicators of inflammation. On the other hand, assessing bleeding upon probing by the standardized probing method with force-controlled probe, would be indicated one of the objective diagnose method of gingivitis.

  30. Two dimensional evaluation by volatile gases for oral condition of diabetes mellitus

    TANDA Naoko, KOSEKI Takeyoshi, HINOKIO Yoshinori, TAKAHASHI Nobuhiro, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2008 - 2010

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    We studied acetone in exhaled air, volatile sulfur compounds and acetone in mouth air, to analyze relation between general condition and oral environment of patients with diabetes mellitus, with healthy volunteers as control. Acetone in exhaled and mouth air of type 2 diabetes patients decreased with improvement of plasma glucose during hospitalization. Study of healthy volunteers with good oral condition showed significantly strong correlation between acetone in exhaled air and acetoacetic acid or 3-hydroxybutyric acid in plasma after fasting overnight. There was no significant correlation between acetone and volatile sulfur compounds. This study suggests possibility that non-invasive acetone measuring indicates ketone bodies in plasma, which reflect pathologic condition of diabetes mellitus.

  31. Development of accelerated remineralization method based upon the non-invasive assessment of mineral density of tooth

    KOSEKI Takeyoshi, TAKAHASHI Nobuhiro, SUZUKI Osamu, TAURA Katsuhiko, IKAWA Kyoko, TANDA Naoko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2008 - 2010

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    Ultrasonic measurement system for mineral density of tooth surfaces had been developed. This system actualized significantly sensitive, non-destructive, and quick measurement. Using this system, we analyzed the relationship between mineral density of tooth and oral environment, especially function of saliva. The result revealed that acid resistivity was correlated with ultrasonic density of tooth mineral. This indicated the possibility of developing new preventive program against tooth decay according to the ultrasonic density of tooth mineral, buffering capacity of saliva, and living practice of daily life.

  32. パウダージェット法と硬組織自己再構成法による瞬時小窩裂溝填塞術の開発

    小関 健由, 佐々木 啓一, 鈴木 治, 田浦 勝彦, 丹田 奈緒子, 細川 亮一, 井川 恭子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的萌芽研究

    Institution: 東北大学

    2008 - 2009

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    パウダージェットデポジション(PJD)法を応用して歯質上に歯の主成分であるハイドロキシアパタイト(HAp)膜の生成し,歯質-生体材料間に全く新しいインターフェイスの創成を実施した。エナメル質の成膜に関しては、PJD装置、HAp微粒子,噴射条件等を検討してきた結果、小窩裂溝内への成膜が確認された。特にPJD装置では、実験用装置ではなく、ハンドピース型装置での成膜が可能になっており、実際の治療に準じたハンドピース先端の噴射ノズルの速度、角度等の条件を自由に設定することにより、より良い成膜条件の検討を可能とした。様々な粒子半径、焼成温度のHAp微粒子を使用し、成膜に適した条件(粒子半径3-4μm,焼成温度1200℃)を明らかにした。さらに、HAp膜の象牙細管透過性へ与える影響を中心に評価を行ったところ、HAp粒子が象牙細管に入り込み、象牙細管開口部を閉鎖し、象牙細管透過性が低下することが判明した。また、条件を変化させることで、PJD装置をそのまま利用して、歯質を切削するアブレイシブジェット加工も行えることが判明した。これらの結果から、今回申請した方法で、軟化象牙質と脱灰エナメル質の選択的除去し清掃された硬組織の実質欠損部分に、PJD法にてハイドロキシアパタイトの歯質と一体化したハイドロキシアパタイト膜を形成させて象牙細管の封鎖と歯髄の保護を行う裏層を施し、その上で充填術を施す全く新しい治療法の可能性が示唆された。

  33. Metabolome analysis of oral biofilm using CE-TOFMS and its application possibility as an oral health indicator

    TAKAHASHI Nobuhiro, HANADA Nobuhiro, KOSEKI Takeyoshi, SENPUKU Hidenobu, SATO Takuichi, SAKAMOTO Mitsuo

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2007 - 2009

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    Metabolome analysis for the representative plaque bacteria and human oral biofilm using a capillary electrophoresis equipped with a time-of-flight mass spectrometer was established. By this method, a new finding was obtained about the pentose-phosphate pathway and the Kreb's tricarboxylic acid cycle, as well as the Embden-Meyerhof-Parnas pathway. In addition, the metabolome analysis was applicable to a very small amount of oral biofilm. These results suggest that the metabolome data can be used as an indicator of oral health status.

  34. Tonometoryによる新しいデジタル歯肉炎診断システム

    井川 恭子, 小関 健由, 井川 資英

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 萌芽研究

    Institution: 東北大学

    2006 - 2007

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    歯肉炎の診査は、発赤・腫脹等の視診による方法、歯周ポケット検査、プロービング時の出血等による方法を使用されることが多い。特に歯肉炎の診査では、若年者が対象であることが多く、侵襲性の少ない診査方法が好ましく、かつ客観性の高い診査方法であることが重要である。 本研究は、トノメーター(i-care^<TM>)改造し、侵襲性の少ない歯肉トノメーター(以下トノメーター)として用い、歯肉炎診査の有用性を検証した。 対象は、健康と思われる成人男性8名(平均年齢24.5歳、ragne:23-27)の可及的健康と思われる前歯部歯肉である。測定対象となる患者からは、前もってインフォームドコンセントを書面で得た。測定装置としてトノメーター(i-care^<TM>)改造し、歯肉トノメーターとして用い、対象者の上下顎前歯部頬側歯間部歯肉の硬度を測定した。対象部位の歯肉溝の深さおよびBOPも同時に測定した。 健常歯肉の硬さは、平均40.8±15.6mmHgで、上顎が下顎より硬いことが示された。歯肉硬さと歯肉溝深さとの相関も示された。 しかし改良型のトノメーターの測定条件からさらなる改良が必要となったことで、再度改良を施した。また、これにより再度測定方法を解析する必要があると考えられ、さらなる研究の必要性が示唆された。

  35. 口腔微生物叢の「揺りかご」:舌苔バイオフィルムの生物学

    高橋 信博, 小関 健由, 佐藤 拓一, 鷲尾 純平, 今井 奨

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 萌芽研究

    Institution: 東北大学

    2005 - 2007

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    本年度は、昨年度に引き続き、口臭原因菌の一つと捉えられる口腔Veillonella属の硫化水素産生活性について検討し、(1)環境因子、とくに口腔内に多く存在する乳酸等の有機酸が硫化水素産生能に与える影響、及び、(2)硫化水素産生に関わる代謝酵素の検索を行った。培養したVeillonella atypica、 V. dispar、 V. parvulaの各標準株(ATCC株)の培養菌にシステインを加えて3時間インキュベートし、産生した硫化水素をメチレンブルー法で定量した。さらにこの反応系に乳酸等の各種有機酸を加え、硫化水素産生活性への影響を検討した。また、培養菌を超音波処理して無細胞抽出液を調整し、システインからの硫化水素産生に関わる酵素活性を検索した。Veillonellaのシステインからの硫化水素産生活性は、乳酸が存在すると著しく増加した。Veillonellaの無細胞抽出液は、システインから硫化水素を産生する酵素活性を示したが、無細胞抽出液に乳酸を添加しても活性の増加は認められなかった。以上のことから、Veillonellaは乳酸の存在下、システインから効率よく硫化水素を産生することが明らかになった。乳酸による促進作用は、酵素の活性化作用ではなく、システインの取込み促進であるものと予測された。口臭源の一つである硫化水素は口腔細菌によって産生されるが、その産生活性はpH(昨年度成果)や有機酸(今年度成果)という口腔環境によって影響を受けるものと考えられ、口腔環境のコントロールによって口臭を抑制し得るものと考えられる。

  36. The study of the association of immune related genotypes with dental caries in Japanese individuals

    CHIBA Junko, KOSEKI Takeyoshi, OZAWA Yuki, HASHIYADA Masaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2005 - 2007

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    The literature is replete with evidence supporting that human dental caries, an infectious disease of bacterial origin, can be preventable by mucosal immunization. Recently, significant genetic contribution to caries progression and severity was reported in a twin study. However, it has not been reported to confirm the association of certain immune related genotypes with caries susceptibility. The purpose of the present study was to confirm the association of certain immune related genotypes with caries susceptibility in Japanese individuals. Human leukocyte antigens (HLAs) play an important role in regulating the immune system. A total of 104 children aged 10 yrs (48 children with caries and 56 children without caries) were included to examine the susceptibility to early onset caries. The HLA・DQB1*03/*05 genotypes were significantly associated with dental caries susceptibility (P=0.005, Pc=0.004). The DRB1*04/*15 genotypes were significantly associated with dental caries susceptibility in a total of 135 children aged 13 yrs (75 children with caries and 60 children without caries) (P=0.0045, Pc=0.04). The HLA-DQB1*06/*06 genotypes were significantly associated with dental caries susceptibility in a total of 164 subjects, aged 26 yrs or younger (mean age, 20.4 ±2.2, 98 adolescents with caries and 66 adolescents without caries) (P=O.0027, Pc=0.027). In conclusion, this study using a cohort of a 10-year old, 13-year-old children, and adolescents with and without caries demonstrated the possible effect of specific HLA genotype on dental caries progression.

  37. レーザー光による初期病変の齲蝕活動性診断器の開発とその臨床的応用の検討

    小関 健由, 藤崎 渉, 内野 正和, 井川 恭子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 萌芽研究

    Institution: 東北大学

    2005 - 2006

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    平成18年度に製作したレーザー光による歯の表面性状解析装置は、読み取り装置に小型CCDエリアセンサと特殊プリズムを応用することにより、臨床応用可能なレベルに小型化したが、実際には歯の表面の曲率に反射光の光軸が大きく影響を受けて、受光面の面出しが容易でなく、システム全体の再考を迫られた。これを解消する目的で反射光のスペックルをスクリーン投影法にて検出すると共に焦点距離を計測する仕組みを加えた装置を組み立て直した。情報処理装置は開発したプログラムを走らせる小型の可搬型コンピュータを用い、歯科健診の会場や日常臨床で使用できる形態に近づけた。さらに、臨床で使用する場合のキャリブレーションを行う標準切片として、各種の研磨法を用いたエナメル質切片を用意した。 う蝕の進行リスクの診断として用いるための、う蝕モデル歯は、倫理規定に基づいて収集した人抜去歯を用い、他のう蝕診断機器を併用してう蝕の進行度を計測しながら化学的実験的平滑面う蝕を製作し、エナメル質表層の性状の変化を計測した。モデル歯に関しては、臨床から得られた無加工のエナメル質は鏡面に近いスペックル値を示すが、実験室で研磨したエナメル質は研磨によってできた傷によるスペックルの乱れが観察され、これは脱灰課程の各切片において変動が大きかった。さらにこの変化は脱灰の方法でも大きく影響が現れた。これらの変化を臨床に応用する場合は、実際の臨床的データとその症例の転帰をあつめた更なる長期的な追跡データベースを構築する必要性が示された。

  38. Establishment of the diagnostic criteria of root caries and the method for its remineralization based on the optical and physical properties of root surfaces

    KOSEKI Takeyoshi, TAKAHASHI Nobuhiro, TAURA Katsuhi, IKAWA Kyoko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2005 - 2006

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    There are no standardized diagnostic criteria of root caries, which occur on the exposed root surface of tooth suffered with periodontitis, especially in elderly people. This absence of evaluation method of decalcification level and mass of soften root surfaces hamper the epidemiological and preventive research of root caries developments. In this study, we evaluated optical measurement with laser and ultrasonic measurement for detecting the mineral loss of root surfaces. Ultrasonic sensor system developed in this study was non-destructive, and small enough to handle in oral cavity. The round shape of front edge of sensor prevents to damage the tooth surfaces during ultrasonic measurements. Furthermore, ultrasonic sensor was placed onto the constant contact-force generator. Then repeatable ultrasonic measurement of decalcification by hand manipulation was achieved. This decalcification detection system of root surface showed reproducible results in considerable clinical conditions, when calibrated with the standards specimen of decalicification. On the other hand, the laser determination method of decalcification was not efficient because root caries and calculus could not be distinguished by using laser fluorescence. These results indicated the diagnostic system of decalcification level of root surfaces will be available by using newly developed non-destructive ultrasonic sensor system. By using this system, the researches of root caries development and preventive method will be proceeded and help to maintain high quality of oral health in elderly people.

  39. Effect of IL-10 over-expression on bone resorption mechanism in apical periodontitis

    SHOJI Noriaki, SASANO Takashi, IIKUBO Masahiro, SUGAWARA Syunji, KOSEKI Kenyoshi, SAKAMOTO Maya

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2004 - 2005

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    Periapical bone resorption occurs following infection of the dental pulp. Bone destruction has been linked to excessive expression of inflammatory cytokines, particularly interleukin (IL)-1. IL-10 is an anti-inflammatory cytokine, produced by various immune cells including macrophages and T2 cells, that we previously found was a key endogeneous inhibitor of infection-stimulated peripheral bone resorption in vivo, likely acting via IL-1 inhibition. More recently, we have shown that IL-10^<-/-> mice are also extremely susceptible Porphyromonas gingivalis (Pg)-stimulated periodontal bone loss, without an increase in gingival IL-1 levels. In contrast, no significant bone loss is induced by the Pg infection in corresponding wild-type (WT) mice. In the present study, we will employ the IL-10^<+/+> mice model, a ‘reverse‘ model of IL-10^<-/-> mice, to test the hypothesis that induction and progression of apical periodontitis can be ameliorated by modulating IL-10, IL-10 receptors their signaling, and cytokine network that IL-10 regulates. The dental pulps of the first molars were exposed and infected with a mixture of four common endodontic pathogens, and bone destruction was determined by micro-computed tomography at sacrifice on day 21. The results demonstrate that IL-10^<+/+> mice model had no significantly greater infection-stimulated bone resorption in vivo compared with wild-type mice, although IL-10 in alveolar bone of them was over-expressed compared with that of wild-type mice.

  40. DNAチップを用いた口腔微生物叢の網羅的定量的プロファイリング

    高橋 信博, 小関 健由, 竹原 直道, 佐藤 拓一

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 萌芽研究

    Institution: 東北大学

    2003 - 2004

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    本年度は、1.前年度設計した口腔細菌特異的プローブを作成し、DNAチップへの搭載の可否(実用性)を検討し、2.DNAチップへの搭載が可能と考えられた24種のプローブを用いて口腔微生物叢解析用DNAチップを試作し、口腔微生物種特異性、使用法などを検討した。 1.DNAチップ搭載用口腔細菌特異的プローブの作成: プローブの特異性についてはGeneBankなどの既存のDNAデータベースから、プローブのDNAチップへの搭載の可否についてはDNAチップ作成手順などの実用性から検討し、その結果、24種の口腔微生物に対する特異的プローブが完成した。さらに、この1年間で新たに入手した口腔微生物16S ribosomal RNA遺伝子配列データに基づき、新たなプローブを設計・作成中である。 2.DNAチップ搭載用の24種の口腔微生物特異的プローブを用いた口腔微生物叢解析用DNAチップの試作: DNAチップ作成共同研究企業にて、24種の口腔微生物特異的プローブを搭載したDNAチップを試作した。各プローブに該当する標準菌株由来DNAを用いて検討したところ、試作したDNAチップは24種の標準菌体由来DNAを特異的に検出することができることがわかった。さらに、DNAチップ上のスポットは肉眼で明瞭に判別でき、市販の可視光用透過型スキャナーで計測・記録することが十分可能であることが分かった。 現在、実際の臨床サンプルを用いて検討中であり、臨床サンプルに含まれる24種の口腔微生物の従来法による検出(通常のnested PCR法での検出)を終え、DNAチップによる検出との照合を進めつつある。

  41. Development of an ultrasonic device to detect early demineralized lesions and establishment of clinical criteria for these diagnoses

    KOSEKI Takeyoshi, IWAKURA Masaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2003 - 2004

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    The onset of dental caries is characterized by white-spots or staining on tooth surfaces. However, estimation of caries activities of these lesions is difficult because we have no tool for recognition which lesion is demineralizing, arresting or re-mineralizing. For detection of the initial changes of the tooth surface affected by dental caries on the enamel and root, we developed a noninvasive, nondestructive dental explorer employing ultrasonic sensor technology. The ultrasonic sensor detection system consists of an oscillating piezoelectric element for sensing, a piezoelectric detective element to obtain frequency characteristics of the tooth surface, and a lightweight arm for positioning the sensor on the tooth surface. Extracted teeth were collected with informed consent and demineralized enamel specimens were prepared with lactate-CMC gel (6%, pH 4.5). The ultrasonic sensor was loaded on these tooth surfaces and scanned for drawing a map of the frequency characteristics. The surface characteristics were represented by the frequency shifts of oscillation in the resonance method. The detecting element of the developed ultrasonic sensor was sufficiently small in the clinic. By measurements using four sets of enamel specimens, early lesions of enamel caries were dearly represented by low frequency shifts of oscillation in a demineralized time-dependent manner. By scanning surfaces of ten extracted teeth, this detection system was able to distinguish sound or caries lesions on the enamel and roots, calculus depositions, staining, white spots and cracks on the enamel surface. However, additional information was need when exploring complex surfaces with overlapping lesions. Our new sensory explorer employing the ultrasonic resonance method was found to be a powerful tool for estimating initial change and degree of progression of dental caries.

  42. STUDY OF THE PATHOGENESIS AND PHYSICAL NATURE OF DENTAL BIOFILM

    TAKEHARA Tadamichi, ANSAI Toshihiro, TAKAHASHI Nobuhiro, KOSEKI Takeyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Kyushu Dental College

    2002 - 2004

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    In the process of developing dental caries on tooth surfaces, characteristic patterns of mineral loss of enamel were observed ahead of collapsing the tooth surface and forming the caries cavity Although this process is affected by microenvironments around the tooth surface, it is difficult to analyze these in detail because of its size and complexity These microenvironments are considered to be determined by several factors, including the presence of microbes and saliva throughout the oral cavity. To understand the microbial factors, we analyzed the interrelationship among the microbes covering the tooth surfaces and host factors which affect the ecology of dental biofilm. Furthermore, to understand the environmental factors, we analyzed biological and physical characteristics of saliva, which play an important role in the microenvironment of the tooth surface. The secretion rates and physical properties of saliva are key factors in the nature of oral circumstances. Thus, physiological properties of saliva, such as viscosity and spinnbarkeit, were analyzed as to their relationship with the pathogenesis and physiological nature of oral cavities. Furthermore, we developed a minute device to obtain data on the microenvironment on tooth surfaces, this device being equipped with semiconductor microprobes for pH and temperature measurement. The memory of this device is sufficiently large to log environmental data for several days. It also includes a highly integrated microcontroller enabling continuous observation of the dynamism of mineral loss. Clinical application of this device to elucidate the nature of the pathogenesis of microbial biofilm will be undertaken to prevent mineral loss due to early dental caries lesions.

  43. Development of prevention method of periodontal diseases by understanding the mechanisms of intracellular invasion by periodontopathic bacteria

    KOSEKI Takeyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    2001 - 2002

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    Actinobacillus actinomycetemcomitans is a Gram-negative, capnophilic coccobacillus which has been considered to be an etiologic agent especially in the severe form of juvenile and adult periodontitis. Previous studies suggested that A.actinomycetemcomitans can invade into periodontal tissues of advanced periodontitis and then survive inside the host cells. We previously showed that apoptotic cell death of the marine macrophage-like cell line was induced by A. actinomycetemcomitans infection. During the course of this apoptosis, activation of several caspases was observed. In general medicine, the facts that severl important pathogenic bacteria can infect and multiple inside the host cells and cause the severe life-threaten infection has been reported. Intracellular invasion mechanisms of some pathogenic bacteria were analyzed in detail, and several caspases has been reported to activate during the course of invasion. We hypothesized that Legionella pneumophila, which causes legion disease, and periodontopathic bacteria, A.actinomycetemcomitans, might invade inside the host cells in similar manner, because both target macrophaees to invade and activate similar set of caspases in host cells. We first adapted the invasion assay designed for A. actinomycetemcomitans to that for L. pneumphila. Mouse macrophage cell line J744.1 and mouse monocyte cell line RAW264.7 are both showed to induce cell death by infection of these bacteria. However, the cell death cased by L. pneumophila was necrosis because the content of cell is filled with multipled bacterial cells just before cell explosion. The difference of the cell death caused by each bacterium might depended on the different signal pathway which are activated by infection even if the similar set of caspases was activated. The RAW264.7 cells which constitutively expressed anti-apopotic Bcl-2 or Bcl-x_L did not rescue cell death by the infection of A. actinomycetemcomitans. Considering all, the mechanisms of invasion and infection of A. actinomycetemcomitans is unique and we need further analysis of these mechanisms for preventing periodontal diseases.

  44. Analysis of Destruction Mechanisms of Dental Biofilm by Laser and Its Clinical Application

    KOSEKI Takeyoshi, OKAHASHI Nobuo, IWAKURA Makaki, NISHIHARA Tatsuji, YAMAMATO Masao, NISHIZAWA Toshiki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    2000 - 2002

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    Dental plaque is the most important etiologic agent of two major dental diseases, dental caries and periodontal diseases. This consists of bacterial cells and their extracellular products and forms the microbial biofilm which adheres sticky to the tooth surfaces. Dental plaque is divided to two parts according to the location to the gingival margin, supragingival plaque and subgingival plaque. Supragingival plaque is easy to remove and to control by toothbrushes expect which is located in interdental area. Subgingival plaque is located inside the periodontal pockets and is difficult to remove completely without visual observation. In the clinics, dental calculus is also major target to eliminate from the tooth surfaces because their rough surfaces are possible retainers of a significant amount of dental plaque. To destroy and to remove dental biofilm and calculus from tooth surfaces completely, we analyzed the physiological resistance of microbial viability of the dental biofilm against the chemical reagents and physiological stresses. We established the assessment method of dental biofilm model to analyze the bactericidal efficiency of various debridement methods. Next, we analyzed the detection method of subgingival calculus by using autofluorescence properties of dental hard tissues. We found subgingival calculus and dentine caries showed characteristic autofluorescence excited by laser irritation, but sound dentine and enamel did not. This method provides us the new diagnosis device for diseased root surfaces without direct visualization. Understanding the physiological properties of dental plaque and calculus, we would established the easy, safe and effective method for tooth cleaning and debridement, to prevent the oral diseases, especially for elderly people who need to care for daily life.

  45. 感染マクロファージのアポトーシスの関与するカスパーゼファミリーの解析

    小関 健由

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 奨励研究(A)

    Institution: 国立感染症研究所

    1999 - 2000

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    若年性歯周炎の病原菌であるActinobacillus actinomycetemcomitansをマクロファージに感染させるとアポトーシスが誘導され、さらにこの菌の産生する新規毒素もマクロファージにアポトーシスを誘導することを我々は報告した。ここで歯周炎発症機序を解析するにあたり、この歯周病原菌によるアポトーシスの細胞内メカニズムの解析が大きな課題となった。一方、細胞のアポトーシスの実行分子は、カスパーゼと呼ばれる蛋白質分解酵素ファミリーであり、細菌の細胞内感染によるアポトーシス発症機序の場合のカスパーゼの関与は、サルモネラ菌および赤痢菌がCaspase-1を活性化してIL-1βの分泌を促進すること、およびレジオネラ菌がCaspase-3を活性化してアポトーシスを誘導することが報告されている。そこで本研究では、下位カスパーゼであるCaspase-1及びCaspase-3を活性化する上位カスパーゼであるCaspase-8及びCaspase-9の両者を検索した。J774.1細胞では、A.actinomycetemcomitansの感染マクロファージのアポトーシスの過程で両者が活性化することが示された。しかしながらどちらのCaspaseの活性化経路が重要であるかは、さらなる検証が必要であることも示された。そこで、A.actinomycetemcomitansの感染マクロファージのアポトーシスの過程をこれまでの実験に使用しているJ774.1細胞に加えて、レジオネラ菌の実験に使用されるマクロファージ細胞株RAW264.7細胞へのA.actinomycetemcomitansの感染実験系を確立した。さらにこのRAW264.7細胞にてアポトーシス抑制効果のあるBcl-2及びBcl-X_Lの形質転換株を作製した。この株を使用して上位のCaspaseの活性化経路を検索し、詳細を見当した。

  46. Analysis of apoptosis in alveolar macrophages induced by periodontopathic bacteria and development of a method of protection from pneumonia

    NISHIHARA Tatsuji, KOSEKI Takeyoshi, KAKINOKI Yasuaki, AKIFUSA Sumio

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B).

    Institution: Kyushu Dental College

    1999 - 2000

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    Infection of murine macrophages in vitro with periodontopathic bacterium Actinobacillus actinomycetemcomitans induces apoptotic cell death. In this study, we investigated the involvement of caspases in apoptotic cell death of A.actinomycetemcomitans-infected macrophages. Two peptide inhibitors of caspases, benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethyl ketone(Z-VAD-FMK)and benzyloxycarbonyl-Asp-Glu-Val-Asp(OMe)-fluoromethyl katone(Z-DEVD-FMK), inhibited apoptotic cell death of mouse macrophage cell line J774.1 infected with A.actinomycetemcomitans. During the process of apoptosis, interleukin-1β(IL-1β) was detected in the culture supernatants of J774.1 cells. IL-1β secretion was blocked by the caspase-1 inhibitor, Z-VAD-FMK, indicating that caspase-1 is involved in not only the induction of apoptosis but also the IL-1β secretion from A.actinomycetemcomintas-infected J774.1 cells. Immunoblot analysis revealed that the infection of A.actinomycetemcomitans to J774.1 cells induced the cleavage of retinoblastoma protein(Rb), suggesting that caspase-3 was activated by A.actinomycetemcomitans-infection. The cytosol from A.actinomycetemcomitans-infected J774.1 cells induced Rb proteollysis in vitro, which was inhibited by the caspase-3 inhibitor, Z-DEVD-FMK.Furthermore, caspase-3-like activity was markedly increased in J774.1 cells infected with A.actinomycetemcomitans between 12h and 24 h, which was subsequently inhibited by the addition of caspase-3 inhibitor, Z-DEVD-FMK.These findings indings indicate that caspase-3 induces apoptosis in J774.1 cells infected with A.actinomycetemcomitans. Taken together, these results suggest that caspase-1 and caspase-3 are involved in the induction of apoptosis in A.actinomycetemcomitans-infected macrophages.

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