Details of the Researcher

PHOTO

Wakako Ohtsubo
Section
Graduate School of Agricultural Science
Job title
Assistant Professor
Degree

  • Doctor of Natural Sciences (Doktor der Naturwissenschaften) (University of Marburg (Philipps-Universität Marburg))

  • Master of Life Sciences (Tohoku University)

  • 学士（農学） (Tohoku University)

e-Rad No.
00598203
Profile

よく知られている環境中の、まだ知られていない微生物の多様性や機能に興味があります。

研究室HP:https://www.agri.tohoku.ac.jp/lacto/

https://tohokuagri.wixsite.com/lapc

Research History 5

  • 2015/04 - Present
    Graduate School of Agricultural Science, Tohoku University　Assistant Professor

  • 2018/09 - 2019/06
    Wageningen University & Research Visiting Researcher

  • 2009/12 - 2015/03
    Tohoku Gakuin University

  • 2008/09 - 2008/11
    Tohoku University　Graduate School of Life Sciences

  • 2004/05 - 2008/08
    Max Planck Institute for Terrestrial Microbiology

Education 4

  • Philipps-University Marburg

    - 2007/12

  • International Max Planck Research School (IMPRS-Mic)

    - 2007/12

  • 東北大学大学院 生命科学研究科 博士課程前期

    - 2004/03

  • Tohoku University　Faculty of Agriculture

    - 2002/03

Committee Memberships 2

  • Frontiers in Microbiology　Topic Editor

    2020 - 2021

  • 日本無菌生物ノートバイオロジー学会　評議員

    2019 -

Professional Memberships 5

  • Japanese Society of Microbial Ecology

  • Japanese Society of Equine Science

    2000/11 - Present

  • JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

  • Japanese Association of Germfree Life and Gnotobiology

  • Japanese Society of Animal Science

Research Interests 14

  • 農芸化学

  • microbial community

  • Animal science

  • Gut microorganisms

  • functional food

  • metagenome

  • anaerobic bacteria

  • prebiotics

  • probiotics

  • Food microbiology

  • gut microbiota

  • Environmental Microbiology

  • Microbial Ecology

  • Applied Microbiology

Research Areas 6

  • Life sciences / Bacteriology /

  • Life sciences / Animal production science /

  • Life sciences / Nutrition and health science /

  • Life sciences / Food sciences / Food Microbiology

  • Life sciences / Applied microbiology /

  • Life sciences / Ecology and environmental science / Microbial Ecology

Awards 4

  1. Murasaki Sendai Hagi Award 2021

    2021/03　Tohoku University　Study of diversity, functionality and application of gut microorganisms

  2. The 33rd Annual Meeting, Prize for Best Presentation

    2020/11　Japanese Society of Equine Science　Interhost dispersal of gut commensal bacteria among horses in the same facility

  3. Presidents Award for Encouragement of Research

    2017/06

  4. 平成28年度先端農学研究奨励賞

    2016/09/12　農学研究科

Papers 69

  1. Aging impairs type 2 immune responses to nematodes associated with reduced gut microbiota responsiveness. International-journal

    Motoko Morimoto, Sota Tanaka, Kyoko Jinguji, Wakako Ikeda-Ohtsubo

    Scientific reports　15　(1)　31385-31385　2025/08/26

    DOI： 10.1038/s41598-025-16730-x 　

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    Gastrointestinal nematode infections elicit robust type 2 immune responses that facilitate rapid parasite expulsion. Our previous studies demonstrated that 18-month-old mice exhibit both impaired nematode clearance and reduced type 2-cytokine production, suggesting that aging diminishes the host's potential to mount effective immune defenses. To further investigate the underlying mechanisms, we compared young (3 months old) and aged mice (18 months old) infected with the nematode Heligmosomoides polygyrus (Hp), focusing on the interplay between type 2 immune responses and intestinal ecology. Hp infected young mice exhibited increased expression of Th2 cytokines (e.g., il-4) and short-chain fatty acid (SCFA) receptors GPR41/GPR43, while these responses were markedly diminished in aged mice. Correspondingly, cecal SCFA levels-particularly acetate and propionate-increased in Hp infected young mice but decreased in aged counterparts. Moreover, Hp infection induced a pronounced shift in the cecal microbiota composition of young mice, notably a reduced Bacillota/Bacteroidota ratio (F/B) ratio, a change much less evident in aged mice. These findings suggest that the age-related decline in type 2 immune responses to gastrointestinal nematode infection is linked to reduced gut microbiota responsiveness, which may compromise host resistance to the gastrointestinal parasites.

  2. Milk sialyl-oligosaccharides mediate the early colonization of gut commensal microbes in piglets. International-journal

    Ryoga Hashimoto, Keita Nishiyama, Fu Namai, Kasumi Suzuki, Taiga Sakuma, Itsuko Fukuda, Yuta Sugiyama, Kenji Okano, Takafumi Shanoh, Eita Toyoshi, Ryusuke Ohgi, Sudeb Saha, Sae Tsuchida, Eri Nishiyama, Takao Mukai, Mutsumi Furukawa, Tomonori Nochi, Julio Villena, Wakako Ikeda-Ohtsubo, Gou Yoshioka, Eri Nakazaki, Yoshihito Suda, Haruki Kitazawa

    Microbiome　13　(1)　135-135　2025/05/24

    DOI： 10.1186/s40168-025-02129-3 　

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    BACKGROUND: The suckling period in pigs is a key phase in development for shaping the gut microbiota, which is essential for maintaining biological homeostasis in neonates. In piglets fed sow milk, the gut microbiota comprises predominantly lactobacilli, indicating a host-gut microbiota symbiosis that is influenced by sow milk components. In this study, we sought to elucidate the mechanisms underlying the establishment and maintenance of the gut microbiome in suckling piglets, with a specific focus on the metabolism of sialyl-oligosaccharides by lactobacilli. RESULTS: Based on liquid chromatography-mass spectrometry analysis, we identified 3'-sialyl-lactose (3'SL) as the major oligosaccharide in porcine milk, and microbiome profiling revealed the predominance of Ligilactobacillus salivarius during the suckling period, with a subsequent transition to Limosilactobacillus reuteri dominance post-weaning. Notably, sialic acid metabolism was established to be exclusively attributable to L. salivarius, thereby highlighting the pivotal role of 3'SL in determining species-specific bacterial segregation. L. salivarius was found to metabolize 3'SL when co-cultured with Bacteroides thetaiotaomicron, resulting in a shift in the predominant short-chain fatty acid produced, from lactate to acetate. This metabolic shift, in turn, inhibits the growth of enterotoxigenic Escherichia coli. Furthermore, the comparison of the gut microbiota between suckling piglets and those fed a low-3'SL formula revealed distinct diversity profiles. We accordingly speculate that an absence of sialyl-oligosaccharides in the formula-fed piglets may have restricted the growth of sialic acid-utilizing bacteria such as L. salivarius, thereby leading to a higher abundance of Enterobacteriaceae. CONCLUSIONS: Our findings reveal the influence of sialyl-oligosaccharides in promoting microbial diversity and gut homeostasis, thereby highlighting the importance of sialic acid as a key factor in shaping milk-driven microbial colonization during the early stages of piglet development. Video Abstract.

  3. Suppression of fecal phenol production by oral supplementation of sesamol: inhibition of tyrosine phenol-lyase by sesamol International-journal

    Daiki Oikawa, Zion Byun, Bunzo Mikami, Aina Gotoh, Toshihiko Katoh, Ryo Ueno, Aruto Nakajima, Satoshi Yamashita, Wakako Ikeda-Ohtsubo, Seiji Takahashi, Toshiyuki Waki, Koichi Kikuchi, Takaaki Abe, Takane Katayama, Toru Nakayama

    Food & Function　16　(9)　3542-3552　2025

    DOI： 10.1039/D4FO04839C 　

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    Phenol is produced from dietary L-tyrosine by the action of tyrosine phenol-lyase (TPL) of gut bacteria and contributes to various physiological disorders, including skin diseases, certain cancers, and kidney dysfunction. We found that oral supplementation of sesamol (36 or 180 μg mL-1) ad libitum for 14 days in mice significantly suppressed fecal phenol production. Fecal microbiota structure analysis in sesamol-supplemented and control groups revealed that their overall microbiota structures were indistinguishable. To explain the sesamol-induced suppression of fecal phenol production, we characterized inhibition of bacterial TPL by sesamol in vitro. Sesamol specifically inhibited bacterial TPL in a mixed-type fashion (Ki, 135 μM), which was rationalized by computational docking studies using the crystal structure of Pantoea agglomerans TPL that was determined at 1.3 Å resolution. Sesamol was detected at 0-0.295 μmol g-1 feces in the sesamol-supplemented group. Given the Ki value of sesamol for TPL inhibition, these levels may not have been sufficient to fully inhibit TPL and suppress fecal phenol production. Therefore, the observed suppression of fecal phenol production upon oral sesamol supplementation arose not solely from the inhibition of TPL by sesamol, but also potentially from the effects of metabolites derived from sesamol and the antioxidant activities of sesamol and related metabolites. Nevertheless, these findings highlight the potential for using sesamol to prevent physiological disorders associated with phenol production by the gut microbiota.

  4. Gut microbiota–mediated lipid accumulation as a driver of evolutionary adaptation to blue light toxicity in Drosophila International-journal

    Yuta Takada, Toshiharu Ichinose, Naoyuki Fuse, Kokoro Saito, Wakako Ikeda-Ohtsubo, Hiromu Tanimoto, Masatoshi Hori

    Communications biology　8　(1)　998-998　2024/08/23

    DOI： 10.1101/2024.08.22.608892 　

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    Human agriculture has always raced against insect adaptation, requiring updated pest control methods and knowledge of evolutionary processes. Excessive exposure to blue light (BL) kills a wide range of insect species and has attracted attention as an alternative to chemical pesticides. Here, to understand how insects adapt to BL toxicity, we investigated evolutionary responses to BL toxicity in Drosophila melanogaster over 70 generations using laboratory selection experiments. The selected line exhibited an obese phenotype accompanied by midgut elongation, with BL tolerance dependent on gut microbiota-mediated lipid accumulation. Whole-genome and transcriptome analyses consistently highlighted interactions between the microbiota and host lipid metabolism-related genes. Remarkably, manipulating genes associated with lipid accumulation conferred BL tolerance even in the absence of selection. We suggest that the acquisition of BL tolerance occurs through 'adaptive obesity'. Our study introduces a mechanism of evolutionary adaptation of insects against BL-based selective pressure by maximising the benefits from the gut microbiota via midgut elongation.

  5. Zebrafish as a Model for the Study of Gut Microbiome

    大坪和香子, 横井勇人

    腸内細菌学雑誌　38　(4)　2024

    ISSN： 1343-0882

  6. Neural control of redox response and microbiota-triggered inflammation in Drosophila gut International-journal

    Naoyuki Fuse, Haruka Hashiba, Kentaro Ishibashi, Takuro Suzuki, Quang-Dat Nguyen, Kiho Fujii, Wakako Ikeda-Ohtsubo, Haruki Kitazawa, Hiromu Tanimoto, Shoichiro Kurata

    Frontiers in Immunology　14　1268611-1268611　2023/10/26

    Publisher: Frontiers Media SA

    DOI： 10.3389/fimmu.2023.1268611 　

    eISSN： 1664-3224

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    Background The neural system plays a critical role in controlling gut immunity, and the gut microbiota contributes to this process. However, the roles and mechanisms of gut-brain-microbiota interactions remain unclear. To address this issue, we employed Drosophila as a model organism. We have previously shown that NP3253 neurons, which are connected to the brain and gut, are essential for resistance to oral bacterial infections. Here, we aimed to investigate the role of NP3253 neurons in the regulation of gut immunity. Methods We performed RNA-seq analysis of the adult Drosophila gut after genetically inactivating the NP3253 neurons. Flies were reared under oral bacterial infection and normal feeding conditions. In addition, we prepared samples under germ-free conditions to evaluate the role of the microbiota in gut gene expression. We knocked down the genes regulated by NP3253 neurons and examined their susceptibility to oral bacterial infections. Results We found that immune-related gene expression was upregulated in NP3253 neuron-inactivated flies compared to the control. However, this upregulation was abolished in axenic flies, suggesting that the immune response was abnormally activated by the microbiota in NP3253 neuron-inactivated flies. In addition, redox-related gene expression was downregulated in NP3253 neuron-inactivated flies, and this downregulation was also observed in axenic flies. Certain redox-related genes were required for resistance to oral bacterial infections, suggesting that NP3253 neurons regulate the redox responses for gut immunity in a microbiota-independent manner. Conclusion These results show that NP3253 neurons regulate the appropriate gene expression patterns in the gut and contribute to maintain homeostasis during oral infections.

  7. Intestinal Microbiota and Immune Modulation in Zebrafish by Fucoidan From Okinawa Mozuku (Cladosiphon okamuranus). International-journal Peer-reviewed

    Wakako Ikeda-Ohtsubo, Adrià López Nadal, Edoardo Zaccaria, Masahiko Iha, Haruki Kitazawa, Michiel Kleerebezem, Sylvia Brugman

    Frontiers in nutrition　7　67-67　2020

    DOI： 10.3389/fnut.2020.00067 　

  8. Recent progress in understanding swine microbiome and its application to development of alternatives to antimicrobial growth promoters (AGP)

    Wakako Ikeda-Ohtsubo, Haruki Kitazawa

    Tohoku Journal of Animal Science and Technology　68　(1)　1-7　2018/06

    Publisher:

    ISSN： 1341-626X

  9. How Can We Define "Optimal Microbiota?": A Comparative Review of Structure and Functions of Microbiota of Animals, Fish, and Plants in Agriculture. International-journal Peer-reviewed

    Wakako Ikeda-Ohtsubo, Sylvia Brugman, Craig H Warden, Johanna M J Rebel, Gert Folkerts, Corné M J Pieterse

    Frontiers in nutrition　5　90-90　2018

    Publisher: Frontiers Media {SA}

    DOI： 10.3389/fnut.2018.00090 　

    ISSN： 2296-861X

  10. 'Candidatus Adiutrix intracellularis', an endosymbiont of termite gut flagellates, is the first representative of a deep-branching clade of Deltaproteobacteria and a putative homoacetogen. International-journal Peer-reviewed

    Wakako Ikeda-Ohtsubo, Jürgen F H Strassert, Tim Köhler, Aram Mikaelyan, Ivan Gregor, Alice C McHardy, Susannah Green Tringe, Phil Hugenholtz, Renate Radek, Andreas Brune

    Environmental microbiology　18　(8)　2548-64　2016/09

    DOI： 10.1111/1462-2920.13234 　

    ISSN： 1462-2912

  11. Characteristics of gut microbiota in high-methylated colorectal cancer.

    Tatsushi Saito, Hideaki Karasawa, Kota Ouchi, Tomoyuki Ono, Taiki Kajiwara, Atsushi Kohyama, Wakako Ikeda-Ohtsubo, Shinji Fukuda, Fumiyoshi Fujishima, Yohei Ozawa, Hideyuki Suzuki, Kazuhiro Watanabe, Chikashi Ishioka, Takashi Kamei, Shinobu Ohnuma, Takaaki Abe, Michiaki Unno

    International journal of clinical oncology　30　(9)　1805-1817　2025/09

    DOI： 10.1007/s10147-025-02812-3 　

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    BACKGROUND: Epigenetic alterations, including DNA methylation, significantly contribute to colorectal cancer (CRC); the gut microbiota is also involved. However, studies on the possible role of microbiota in DNA methylation are limited. This study investigates the association between gut microbiota composition and high-methylated CRC (HMCC). METHODS: Fecal and tumor tissue samples were collected from 86 patients with sporadic CRC. HMCC was defined based on the methylation status of 16 CpG sites of tumor-derived genomic DNA. 16S rRNA gene sequencing was performed to reveal the composition of the gut microbiota. The load of Fusobacterium nucleatum (F. nucleatum) in tumor tissues was assessed using quantitative polymerase chain reaction (qPCR). RESULTS: HMCC was identified in 21 patients, whereas 65 were classified as having low-methylated CRC (LMCC). HMCC was significantly associated with proximal location, large diameter, undifferentiated histology, and high frequency of BRAF mutation. In gut microbial analyses, the relative abundances of 84 bacteria, including F. nucleatum, were significantly different between HMCC and LMCC. The load of F. nucleatum in CRC specimens was significantly correlated with its relative abundance in fecal samples and tended to be enriched in HMCC tissues. CONCLUSIONS: This study characterizes the gut microbiota profile in HMCC and suggests that bacteria, such as F. nucleatum, may contribute to HMCC pathogenesis through DNA methylation. Further studies are needed to determine whether the microbiome acts as a promoter or bystander in HMCC development.

  12. The Originally Established PBE Cell Line as a Reliable In Vitro Model for Investigating SIV Infection and Immunity. International-journal

    Xi-Chen Bai, Kohtaro Fukuyama, Leonardo Albarracin, Yoshiya Imamura, Fu Namai, Weichen Gong, Wakako Ikeda-Ohtsubo, Keita Nishiyama, Julio Villena, Haruki Kitazawa

    International journal of molecular sciences　26　(12)　2025/06/16

    DOI： 10.3390/ijms26125764 　

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    Previously, we developed a porcine bronchial epithelial cell line designated as PBE cells and demonstrated that this cell line possesses functional Toll-like receptor 3 (TLR3), triggering the expressions of interferons (IFNs), antiviral factors, and inflammatory cytokines after its stimulation with the synthetic double-stranded ARN poly(I:C). In this work, we aimed to further characterize the PBE cell line as a reliable in vitro model for investigating swine influenza virus (SIV) infection and immunity. We evaluated the capacity of two SIV subtypes, H1N1 and H3N2, to replicate and induce cytopathic effects in PBE cells and to modulate the expressions of IFNs, antiviral factors, inflammatory cytokines, and negative regulators of the TLR signaling. We demonstrated that PBE cells are susceptible to both H1N1 and H3N2. SIV infected PBE cells inducing notable cytopathic effects as shown by the alteration of transepithelial electrical resistance (TEER) and cilia. Both SIV subtypes replicated in PBE cells in similar proportion and altered TEER values in comparable magnitudes. However, SIV H3N2 induced higher alterations of cilia than H1N1. SIV infection induced changes in all the immune factors evaluated in PBE cells. We detected quantitative differences when the subtypes H1N1 and H3N2 were compared. The fold expression changes of IFN-β, Mx1, Mx2, IFITM1, OAS1, OAS2, and OASL were higher in PBE cells infected with H3N2 than in cells challenged with H1N1. In addition, although both subtypes stimulated IL-8 expression, only the H3N2 induced IL-6 in infected PBE cells. SIV H1N1 and H3N2 also upregulated the expressions of the negative regulators A20, BCL-3, and MKP-1, while only H1N1 increased SIGIRR and Tollip. Immortalized respiratory cell lines from pigs can be useful in vitro systems for the study of viral infections and immune responses. These studies are of importance in the context of influenza infections not only for the agricultural field because pigs are natural hosts of these viruses but also because these animals serve as intermediate reservoirs of viruses that can threaten humans' health. We demonstrated here that the PBE cell line can be a useful in vitro model to study SIV infection and immunity.

  13. Exploring strain-level diversity in the gut microbiome through mucin particle adhesion. International-journal

    Keita Nishiyama, Ryuta Murakami, Masaki Nakahata, Binghui Zhou, Nanami Hashikura, Hiroki Kaneko, Fu Namai, Wakako Ikeda-Ohtsubo, Jin-Zhong Xiao, Haruki Kitazawa, Toshitaka Odamaki

    Applied and environmental microbiology　90　(9)　e0123524　2024/08/12

    DOI： 10.1128/aem.01235-24 　

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    UNLABELLED: Mucin glycoproteins are a significant source of carbon for the gut bacteria. Various gut microbial species possess diverse hydrolytic enzymes and catabolic pathways for breaking down mucin glycans, resulting in competition for the limited nutrients within the gut environment. Adherence to mucin glycans represents a crucial strategy used by gut microbes to access nutrient reservoirs. Understanding these properties is pivotal for comprehending the survival mechanisms of bacteria in the gastrointestinal tract. However, characterization of individual strains within the vast array of coexisting bacteria in the microbiome is challenging. To investigate this, we developed mucin-immobilized particles by immobilizing porcine gastric mucin (PGM) onto glass beads chemically modified with boronic acid. These PGM-immobilized particles were then anaerobically cultured with human fecal microbiota, and the bacteria adhering to PGM were isolated. Interestingly, the microbiome composition remained largely unchanged irrespective of PGM immobilization. Nonetheless, bacteria isolated from PGM-immobilized glass particles exhibited notably higher N-acetylgalactosaminidase activity compared to the control beads. Furthermore, Bacteroides strains isolated from PGM-immobilized glass particles displayed enhanced adhesive and metabolic properties to PGM. These findings underscore the utility of PGM particles in enriching and isolating specific microbes. Moreover, they highlight substantial differences in microbial properties at the strain level. We anticipate that PGM-immobilized particles will advance culture-based microbiome research, emphasizing the significance of strain-level characterization. IMPORTANCE: Metabolism of mucin glycans by gut bacteria represents a crucial strategy for accessing nutrient reservoirs. The efficacy of mucin glycan utilization among gut bacteria hinges on the metabolic capabilities of individual strains, necessitating meticulous strain-level characterization. In this investigation, we used glass beads chemically immobilized with mucins to selectively enrich bacteria from fecal fermentation cultures, based on their superior adhesion to and metabolism of mucin glycoproteins. These findings lend support to the hypothesis that the physical interactions between bacteria and mucin glycoprotein components directly correlate with their capacity to utilize mucins as nutrient sources. Furthermore, our study implies that physical proximity may significantly influence bacterial nutrient acquisition within the ecosystem, facilitating gut bacteria's access to carbohydrate components.

  14. Broad specificity of monoclonal IgA (TEPC15-IgA) for enteric bacteria via phosphorylcholine-mediated interaction.

    Saeka Koyama, Kaori Ito, Katsuki Usami, Shino Wada, Tsukasa Yamashita, Wakako Ikeda-Ohtsubo, Haruki Kitazawa, Ryota Hirakawa, Jahidul Islam, Mutsumi Furukawa, Tomonori Nochi

    The Journal of veterinary medical science　86　(7)　801-808　2024/06/04

    DOI： 10.1292/jvms.23-0441 　

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    Immunoglobulin A (IgA) is notable for its broad specificity toward multiple bacteria. Phosphorylcholine (PC) plays a role in the infection of pathogenic bacteria carrying PC and in the induction of IgA responses in the host immune system. The commercially available mouse monoclonal IgA, TEPC15-IgA, is a distinctive antibody with specificity for PC, warranting further exploration of its response to PC-bearing enteric bacteria. In this study, using 17 different enteric bacteria, including 3 aerobic and 14 anerobic bacteria that could be cultured in vitro, we confirmed that TEPC15-IgA recognizes 4 bacterial species: Lactobacillus taiwanensis, Limosilactobacillus frumenti, Streptococcus infantis, and Escherichia coli, although reactivity varied. Interestingly, TEPC15-IgA did not react with four of six Lactobacillus species used. Moreover, distinct target molecules associated with PC in L. taiwanensis and L. frumenti were evident, differing in molecular weight. These findings suggest that the natural generation of PC-specific IgA could prevent PC-mediated infections and potentially facilitate the formation of a microflora rich in indigenous bacteria with PC, particularly in the gastrointestinal tract.

  15. Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses

    Kaho MATSUMOTO, Fu NAMAI, Ayako MIYAZAKI, Yoshiya IMAMURA, Kohtaro FUKUYAMA, Wakako IKEDA-OHTSUBO, Keita NISHIYAMA, Julio VILLENA, Kohtaro MIYAZAWA, Haruki KITAZAWA

    Bioscience of Microbiota, Food and Health　43　(4)　342-351　2024

    Publisher: BMFH Press

    DOI： 10.12938/bmfh.2024-0046 　

    eISSN： 2186-3342

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    Intestinal homeostasis and integrity are important factors for maintaining host health. This study established intestinal epithelial cell lines and organoids from the same swine jejunal crypts to develop seamless swine intestinal in vitro evaluation systems. The study evaluated the proliferative capacity and tight junction formation of the epithelial cell line and characterized the cell differentiation potential of the intestinal organoids. The evaluation systems were subsequently exposed to the Toll-like receptor 3 (TLR3) agonist poly(I:C) to simulate viral infections and assess the antiviral responses. The results demonstrated no differences in the response to type I interferons. There were, however, significant differences in the expression of interferon-stimulated genes. This study collectively introduced a flexible evaluation system using cell lines and organoids and revealed notable differences in the expression of interferon-stimulated genes, highlighting the complexity of the immune responses in these in vitro systems and the importance of intestinal heterogeneity in assessing viral responses.

  16. In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk. International-journal

    Ryusuke Ohgi, Sudeb Saha, Binghui Zhou, Taiga Sakuma, Mitsuki Sakurai, Yuhka Nakano, Fu Namai, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Keita Nishiyama, Julio Villena, Haruki Kitazawa

    Frontiers in microbiology　15　1324999-1324999　2024

    DOI： 10.3389/fmicb.2024.1324999 　

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    The emergence and spread of antibiotic resistance threat forced to explore alternative strategies for improving the resistance to pathogens in livestock production. Probiotic lactic acid bacteria represent an alternative for this objective. In this study, seven Lactiplantibacillus plantarum strains from porcine colostrum and milk were isolated, identified and characterized in terms of their abilities to modulate immunity in porcine intestinal epithelial (PIE) cells. Then, two potential immunoregulatory strains were studied in terms of their ability to utilize and grow in wakame (Undaria pinnafida). Isolates were identified by 16S rRNA gene and evaluated by studying their interaction with PIE cells. The expressions of peptidoglycan recognition proteins (PGRPs), nucleotide-binding oligomerization domain (NODs), host defense peptides (pBD), and type I interferons (IFNs) were evaluated by RT-qPCR. The strain 4M4417 showed a remarkable capacity to differentially regulate the expression of PGRP1, PGRP3, NOD1, NOD2, and pBD1 in PIE cells. On the other hand, the strain 4M4326 was the most efficient to improve the expression of IFN-α and IFN-β in PIE cells challenged with poly (I:C). Both L. plantarum 4M4326 and 4M4417 were characterized in terms of their ability to utilize wakame. Results demonstrated that both strains efficiently grew in wakame-based broth. Our results suggest that L. planatrum 4M4326 and 4M4417 are interesting candidates to develop immunomodulatory feeds based on wakame utilization. These new immunosynbiotic feeds could help to reduce severity of intestinal infections and improve immune health status in pigs.

  17. A simple antigen-antibody reaction using ultrasmall FeCo nanoparticles

    Loi Tonthat, Toru Murayama, Nobukiyo Kobayashi, Shin Yabukami, Wakako Ikeda-Ohtsubo, Ken Ichi Arai

    AIP Advances　13　(2)　2023/02/01

    Publisher: AIP Publishing

    DOI： 10.1063/9.0000569 　

    eISSN： 2158-3226

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    In this study, we prepared ultrasmall FeCo nanoparticles (NPs) with a high magnetic moment and examined their antigen-antibody reaction for biodetection applications. The FeCo NPs were collected from the FeCo-BaF2 nanogranular film with Fe:Co:Ba:F = 14:11:21:54 at.%, by dissolving the film in water since the BaF2 matrix was deliquescent. The size of FeCo NPs was ∼5 nm and the saturation magnetization was estimated to be ∼15.30 kG (149.0 emu/g). The Candida albicans antibodies (abcam ab53891)-conjugated FeCo NPs were collected by using an ultracentrifugal separation (110 000 rpm, 90 min), they were then reacted with Candida albicans. The obtained result indicates that Candida albicans were absorbed successfully onto FeCo NPs, and the number of Candida albicans bound to FeCo NPs counted from the micrographs of the aggregates of FeCo NPs and Candida albicans increased significantly by adding sonication treatment of the film in water before binding them to the antibodies. The success of antigen-antibody reaction of ultrasmall NPs with high magnetic moment improves detection sensitivity as well as offers potential detection for smaller biomolecules.

  18. Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium. International-journal

    Kohtaro Fukuyama, Tao Zhuang, Eita Toyoshi, Fernanda Raya Tonetti, Sudeb Saha, Binghui Zhou, Wakako Ikeda-Ohtsubo, Keita Nishiyama, Hisashi Aso, Julio Villena, Haruki Kitazawa

    Frontiers in immunology　14　1117102-1117102　2023

    DOI： 10.3389/fimmu.2023.1117102 　

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    In vitro culture models that precisely mirror the porcine respiratory epithelium are needed to gain insight into how pathogens and host interact. In this study, a new porcine bronchial epithelial cell line, designated as PBE cells, was established from the respiratory tract of a neonatal pig. PBE cells assumed a cobblestone-epithelial like morphology with close contacts between the cells when they reached confluence. The PBE cell line was characterized in terms of its expression of pattern recognition receptors (PRRs) and its ability to respond to the activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways, which are key PRRs involved in the defense of the respiratory epithelium against pathogens. PBE cells stimulated with poly(I:C) were able to up-regulate the expression of IFN-β, IFN-λ1 (IL-29), IFN-λ3 (IL-28B), the antiviral factors Mx1, OAS1, and PKR, as well as the viral PRRs RIG-1 and MDA5. The expression kinetics studies of immune factors in PBE cells allow us to speculate that this cell line can be a useful in vitro tool to investigate treatments that help to potentiate antiviral immunity in the respiratory epithelium of the porcine host. In addition, poly(I:C) and LPS treatments increased the expression of the inflammatory cytokines TNF-α, IL-6, IL-8, and MCP-1/CCL2 and differentially modulated the expression of negative regulators of the TLR signaling pathways. Then, PBE cells may also allow the evaluation of treatments that can regulate TLR3- and TLR4-mediated inflammatory injury in the porcine airway, thereby protecting the host against harmful overresponses.

  19. The Mucus Binding Factor Is Not Necessary for Lacticaseibacillus rhamnosus CRL1505 to Exert Its Immunomodulatory Activities in Local and Distal Mucosal Sites. International-journal

    Kae Tomotsune, Fernanda Raya Tonetti, Hiroya Mizuno, Mariano Elean, Kohtaro Fukuyama, Binghui Zhou, Wakako Ikeda-Ohtsubo, Keita Nishiyama, Akihiro Yamamura, Hideaki Karasawa, Shinobu Ohnuma, Akira Horii, Tadao Saito, Haruki Kitazawa, Julio Villena

    International journal of molecular sciences　23　(22)　2022/11/18

    DOI： 10.3390/ijms232214357 　

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    Both viable and non-viable orally administered Lacticaseibacillus rhamnosus CRL1505 modulate immunity in local (intestine) and distal (respiratory) mucosal sites. So, intestinal adhesion and colonization are not necessary for this probiotic strain to exert its immunomodulatory effects. In this work, a mucus-binding factor knockout CRL1505 strain (ΔmbfCRL1505) was obtained and the lack of binding ability to both intestinal epithelial cells and mucin was demonstrated in vitro. In addition, two sets of in vivo experiments in 6-week-old Balb/c mice were performed to evaluate ΔmbfCRL1505 immunomodulatory activities. (A) Orally administered ΔmbfCRL1505 prior to intraperitoneal injection of the Toll-like receptor 3 (TLR3) agonist poly(I:C) significantly reduced intraepithelial lymphocytes (CD3+NK1.1+CD8αα+) and pro-inflammatory mediators (TNF-α, IL-6 and IL-15) in the intestinal mucosa. (B) Orally administered ΔmbfCRL1505 prior to nasal stimulation with poly(I:C) significantly decreased the levels of the biochemical markers of lung tissue damage. In addition, reduced recruitment of neutrophils and levels of pro-inflammatory mediators (TNF-α, IL-6 and IL-8) as well as increased IFN-β and IFN-γ in the respiratory mucosa were observed in ΔmbfCRL1505-treated mice when compared to untreated control mice. The immunological changes induced by the ΔmbfCRL1505 strain were not different from those observed for the wild-type CRL1505 strain. Although it is generally accepted that the expression of adhesion factors is necessary for immunobiotics to induce their beneficial effects, it was demonstrated here that the mbf protein is not required for L. rhamnosus CRL1505 to exert its immunomodulatory activities in local and distal mucosal sites. These results are a step forward towards understanding the mechanisms involved in the immunomodulatory capabilities of L. rhamnosus CRL1505.

  20. Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity International-journal International-coauthorship Peer-reviewed

    Mikado Tomokiyo, Fernanda Raya-Tonetti, Hikari Yamamuro, Ryoko Shibata, Kohtaro Fukuyama, Nadia Gobbato, Leonardo Albarracin, Muhammad Shahid Riaz Rajoka, A. K. M. Humayun Kober, Wakako Ikeda-Ohtsubo, Julio Villena, Haruki Kitazawa

    Cells　11　(2986)　1-23　2022/09/25

    Publisher: MDPI

    DOI： 10.3390/cells11192986 　

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    IF = 7.666

  21. Simplified Fabrication of Magnetic Nanoparticles With Directly Adsorbed Antibodies for Bacteria Detection

    Tsuyoshi Yoneyama, Akihiro Kuwahata, Toru Murayama, Loi Tonthat, Shin Yabukami, Yuya Sato, Yuji Teramura, Wakako Ikeda-Ohtsubo, Tomoyuki Ogawa

    IEEE Transactions on Magnetics　58　(8)　1-6　2022/08

    Publisher: Institute of Electrical and Electronics Engineers (IEEE)

    DOI： 10.1109/tmag.2022.3168360 　

    ISSN： 0018-9464

    eISSN： 1941-0069

  22. Soymilk-fermented with Lactobacillus delbrueckii subsp. delbrueckii TUA4408L improves immune-health in pigs. International-journal

    Y Suda, K Kagawa, K Fukuyama, M Elean, B Zhou, M Tomokiyo, M Aminul Islam, M S R Rajoka, A K M Humayun Kober, T Shimazu, S Egusa, Y Terashima, H Aso, W Ikeda-Ohtsubo, J Villena, H Kitazawa

    Beneficial microbes　1-12　2022/01/31

    DOI： 10.3920/BM2021.0068 　

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    Lactobacillus delbrueckii subsp. delbrueckii TUA4408L has the ability to grow and ferment soymilk and is able to modulate the innate immune response of intestinal epithelial cells in vitro. These two properties prompt us to evaluate whether the soymilk fermented with the TUA4408L strain can induce beneficial immunomodulatory effects in vivo. For this purpose, pigs were selected as a preclinical model. The studies performed here demonstrated that the L. delbrueckii subsp. delbrueckii TUA4408L-fermented soymilk (TUA4408L FSM) reduced blood markers of inflammation and differentially regulated the expression of inflammatory and regulatory cytokines in the intestinal mucosa. These immunological changes induced by the TUA4408L FSM were associated to an enhanced resistance to pathogenic Escherichia coli and an improved grow performance and meat quality of pigs. The experiments and analysis in our study indicate that the immunobiotic TUA4408L FSM could be an interesting non-dairy functional food to beneficially modulate the intestinal immune system, improve protection against pathogens and reduce inflammatory damage. The preclinical study carried out here in pigs could have a better correlation in humans, compared to a rodent model. However, the clinical relevance of these findings still needs to be confirmed by further research, for example, in controlled human challenge studies.

  23. 変わりゆく細菌学名との上手な付き合い方

    大坪 和香子

    生物工学会誌　100　(1)　43-43　2022/01/25

    Publisher: 公益社団法人 日本生物工学会

    DOI： 10.34565/seibutsukogaku.100.1_43 　

    ISSN： 0919-3758

    eISSN： 2435-8630

  24. SGLT-1-specific inhibition ameliorates renal failure and alters the gut microbial community in mice with adenine-induced renal failure. International-journal

    Hsin-Jung Ho, Koichi Kikuchi, Daiki Oikawa, Shun Watanabe, Yoshitomi Kanemitsu, Daisuke Saigusa, Ryota Kujirai, Wakako Ikeda-Ohtsubo, Mariko Ichijo, Yukako Akiyama, Yuichi Aoki, Eikan Mishima, Yoshiaki Ogata, Yoshitsugu Oikawa, Tetsuro Matsuhashi, Takafumi Toyohara, Chitose Suzuki, Takehiro Suzuki, Nariyasu Mano, Yoshiteru Kagawa, Yuji Owada, Takane Katayama, Toru Nakayama, Yoshihisa Tomioka, Takaaki Abe

    Physiological reports　9　(24)　e15092　2021/12

    DOI： 10.14814/phy2.15092 　

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    Sodium-dependent glucose cotransporters (SGLTs) have attracted considerable attention as new targets for type 2 diabetes mellitus. In the kidney, SGLT2 is the major glucose uptake transporter in the proximal tubules, and inhibition of SGLT2 in the proximal tubules shows renoprotective effects. On the other hand, SGLT1 plays a role in glucose absorption from the gastrointestinal tract, and the relationship between SGLT1 inhibition in the gut and renal function remains unclear. Here, we examined the effect of SGL5213, a novel and potent intestinal SGLT1 inhibitor, in a renal failure (RF) model. SGL5213 improved renal function and reduced gut-derived uremic toxins (phenyl sulfate and trimethylamine-N-oxide) in an adenine-induced RF model. Histological analysis revealed that SGL5213 ameliorated renal fibrosis and inflammation. SGL5213 also reduced gut inflammation and fibrosis in the ileum, which is a primary target of SGL5213. Examination of the gut microbiota community revealed that the Firmicutes/Bacteroidetes ratio, which suggests gut dysbiosis, was increased in RF and SGL5213 rebalanced the ratio by increasing Bacteroidetes and reducing Firmicutes. At the genus level, Allobaculum (a major component of Erysipelotrichaceae) was significantly increased in the RF group, and this increase was canceled by SGL5213. We also measured the effect of SGL5213 on bacterial phenol-producing enzymes that catalyze tyrosine into phenol, following the reduction of phenyl sulfate, which is a novel marker and a therapeutic target for diabetic kidney disease DKD. We found that the enzyme inhibition was less potent, suggesting that the change in the microbial community and the reduction of uremic toxins may be related to the renoprotective effect of SGL5213. Because SGL5213 is a low-absorbable SGLT1 inhibitor, these data suggest that the gastrointestinal inhibition of SGLT1 is also a target for chronic kidney diseases.

  25. Immunobiotic Feed Developed with Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and the Soymilk By-Product Okara Improves Health and Growth Performance in Pigs. International-journal

    Yoshihito Suda, Nana Sasaki, Kyoma Kagawa, Mariano Elean, Binghui Zhou, Mikado Tomokiyo, Md Aminul Islam, Muhammad Shahid Riaz Rajoka, A K M Humayun Kober, Tomoyuki Shimazu, Shintaro Egusa, Yuji Terashima, Hisashi Aso, Wakako Ikeda-Ohtsubo, Julio Villena, Haruki Kitazawa

    Microorganisms　9　(5)　2021/04/25

    DOI： 10.3390/microorganisms9050921 　

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    Lactobacillus delbrueckii subsp. delbrueckii TUA4408L is able to differentially modulate the innate immune response of porcine intestinal epithelial cells triggered by TLR4 activation. This strain also has a remarkable ability to grow on plant substrates. These two immunological and biotechnological characteristics prompted us to evaluate whether the soymilk by-product okara fermented with the TUA4408L strain can serve as an immunobiotic feed with the ability to beneficially modulate the intestinal immunity of piglets after weaning to improve their productivity. Our in vivo studies demonstrated that the administration of immunobiotic TUA4408L-fermented okara feed significantly increased piglet growth performance and meat quality. These positive effects were associated with the ability of the TUA4408L-fermented okara feed to beneficially modulate both intestinal microbiota and immunity in pigs. The immunobiotic feed improved the abundance of the beneficial bacteria Lactobacillus and Lactococcus in the gut of pigs, reduced blood markers of inflammation, and differentially regulated the expression of inflammatory and regulatory cytokines in the intestinal mucosa. These findings indicate that the immunobiotic TUA4408L-fermented okara feed could be an economical and environmentally friendly option to improve the growth performance and immune health of pigs.

  26. Ligilactobacillus salivarius Strains Isolated From the Porcine Gut Modulate Innate Immune Responses in Epithelial Cells and Improve Protection Against Intestinal Viral-Bacterial Superinfection. International-journal

    Yuhki Indo, Shugo Kitahara, Mikado Tomokiyo, Shota Araki, Md Aminul Islam, Binghui Zhou, Leonardo Albarracin, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Takato Takenouchi, Hirohide Uenishi, Hisashi Aso, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa

    Frontiers in immunology　12　652923-652923　2021

    DOI： 10.3389/fimmu.2021.652923 　

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    Previously, we constructed a library of Ligilactobacillus salivarius strains from the intestine of wakame-fed pigs and reported a strain-dependent capacity to modulate IFN-β expression in porcine intestinal epithelial (PIE) cells. In this work, we further characterized the immunomodulatory activities of L. salivarius strains from wakame-fed pigs by evaluating their ability to modulate TLR3- and TLR4-mediated innate immune responses in PIE cells. Two strains with a remarkable immunomodulatory potential were selected: L. salivarius FFIG35 and FFIG58. Both strains improved IFN-β, IFN-λ and antiviral factors expression in PIE cells after TLR3 activation, which correlated with an enhanced resistance to rotavirus infection. Moreover, a model of enterotoxigenic E. coli (ETEC)/rotavirus superinfection in PIE cells was developed. Cells were more susceptible to rotavirus infection when the challenge occurred in conjunction with ETEC compared to the virus alone. However, L. salivarius FFIG35 and FFIG58 maintained their ability to enhance IFN-β, IFN-λ and antiviral factors expression in PIE cells, and to reduce rotavirus replication in the context of superinfection. We also demonstrated that FFIG35 and FFIG58 strains regulated the immune response of PIE cells to rotavirus challenge or ETEC/rotavirus superinfection through the modulation of negative regulators of the TLR signaling pathway. In vivo studies performed in mice models confirmed the ability of L. salivarius FFIG58 to beneficially modulate the innate immune response and protect against ETEC infection. The results of this work contribute to the understanding of beneficial lactobacilli interactions with epithelial cells and allow us to hypothesize that the FFIG35 or FFIG58 strains could be used for the development of highly efficient functional feed to improve immune health status and reduce the severity of intestinal infections and superinfections in weaned piglets.

  27. Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies. International-journal

    Binghui Zhou, Leonardo Albarracin, Yuhki Indo, Lorena Arce, Yuki Masumizu, Mikado Tomokiyo, Md Aminul Islam, Valeria Garcia-Castillo, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Hidetoshi Morita, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa

    Microorganisms　8　(11)　2020/10/26

    DOI： 10.3390/microorganisms8111659 　

  28. Draft Genome Sequence of Ligilactobacillus salivarius FFIG58, Isolated from the Intestinal Tract of Wakame-Fed Pig. International-journal

    Binghui Zhou, Leonardo Albarracin, Yuki Masumizu, Yuhki Indo, M Aminul Islam, Valeria Garcia-Castillo, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Julio Villena, Haruki Kitazawa

    Microbiology resource announcements　9　(34)　2020/08/20

    Publisher: American Society for Microbiology

    DOI： 10.1128/MRA.00839-20 　

  29. Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics. International-journal Peer-reviewed

    Asuka Tada, Akm Humayun Kober, Md Aminul Islam, Manami Igata, Michihiro Takagi, Masahiko Suzuki, Hisashi Aso, Wakako Ikeda-Ohtsubo, Kazutoyo Yoda, Kenji Miyazawa, Fang He, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Cells　9　(7)　2020/07/17

    DOI： 10.3390/cells9071715 　

  30. Erratum: Fukuyama, K., et al. Evaluation of the Immunomodulatory Ability of Lactic Acid Bacteria Isolated from Feedlot Cattle Against Mastitis Using a Bovine Mammary Epithelial Cells In Vitro Assay. Pathogens 2020, 9, 410. International-journal Peer-reviewed

    Kohtaro Fukuyama, Md Aminul Islam, Michihiro Takagi, Wakako Ikeda-Ohtsubo, Shoichiro Kurata, Hisashi Aso, Maria Elena Fatima Nader-Macías, Graciela Vignolo, Julio Villena, Haruki Kitazawa

    Pathogens (Basel, Switzerland)　9　(7)　2020/07/16

    DOI： 10.3390/pathogens9070574 　

  31. Evaluation of the Immunomodulatory Ability of Lactic Acid Bacteria Isolated from Feedlot Cattle Against Mastitis Using a Bovine Mammary Epithelial Cells In Vitro Assay. International-journal Peer-reviewed

    Kohtaro Fukuyama, Md Aminul Islam, Michihiro Takagi, Wakako Ikeda-Ohtsubo, Shoichiro Kurata, Hisashi Aso, Graciela Vignolo, Julio Villena, Haruki Kitazawa

    Pathogens (Basel, Switzerland)　9　(5)　2020/05/25

    DOI： 10.3390/pathogens9050410 　

  32. Transcriptome Analysis of The Inflammatory Responses of Bovine Mammary Epithelial Cells: Exploring Immunomodulatory Target Genes for Bovine Mastitis. International-journal Peer-reviewed

    Md Aminul Islam, Michihiro Takagi, Kohtaro Fukuyama, Ryoya Komatsu, Leonardo Albarracin, Tomonori Nochi, Yoshihito Suda, Wakako Ikeda-Ohtsubo, Victor Rutten, Willem van Eden, Julio Villena, Hisashi Aso, Haruki Kitazawa

    Pathogens (Basel, Switzerland)　9　(3)　2020/03/09

    DOI： 10.3390/pathogens9030200 　

  33. Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin. International-journal Peer-reviewed

    Asuka Tada, Md Aminul Islam, Akm Humayun Kober, Kohtaro Fukuyama, Michihiro Takagi, Manami Igata, Leonardo Albarracin, Wakako Ikeda-Ohtsubo, Kenji Miyazawa, Kazutoyo Yoda, Fang He, Hideki Takahashi, Julio Villena, Hisashi Aso, Haruki Kitazawa

    International journal of molecular sciences　21　(2)　2020/01/18

    DOI： 10.3390/ijms21020638 　

  34. Immunobiotic Lactobacillus jensenii TL2937 Alleviates Dextran Sodium Sulfate-Induced Colitis by Differentially Modulating the Transcriptomic Response of Intestinal Epithelial Cells. International-journal

    Nana Sato, Valeria Garcia-Castillo, Mao Yuzawa, Md Aminul Islam, Leonardo Albarracin, Mikado Tomokiyo, Wakako Ikeda-Ohtsubo, Apolinaria Garcia-Cancino, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Frontiers in immunology　11　2174-2174　2020

    DOI： 10.3389/fimmu.2020.02174 　

  35. Exopolysaccharides From Streptococcus thermophilus ST538 Modulate the Antiviral Innate Immune Response in Porcine Intestinal Epitheliocytes. International-journal Peer-reviewed

    Hiroya Mizuno, Kae Tomotsune, Md Aminul Islam, Ryutaro Funabashi, Leonardo Albarracin, Wakako Ikeda-Ohtsubo, Hisashi Aso, Hideki Takahashi, Katsunori Kimura, Julio Villena, Yasuko Sasaki, Haruki Kitazawa

    Frontiers in microbiology　11　894-894　2020

    DOI： 10.3389/fmicb.2020.00894 　

  36. Feed, Microbiota, and Gut Immunity: Using the Zebrafish Model to Understand Fish Health. International-journal Peer-reviewed

    Adrià López Nadal, Wakako Ikeda-Ohtsubo, Detmer Sipkema, David Peggs, Charles McGurk, Maria Forlenza, Geert F Wiegertjes, Sylvia Brugman

    Frontiers in immunology　11　114-114　2020

    DOI： 10.3389/fimmu.2020.00114 　

  37. Paraimmunobiotic Bifidobacteria Modulate the Expression Patterns of Peptidoglycan Recognition Proteins in Porcine Intestinal Epitheliocytes and Antigen Presenting Cells. International-journal Peer-reviewed

    Hikaru Iida, Masanori Tohno, Md Aminul Islam, Nana Sato, Hisakazu Kobayashi, Leonardo Albarracin, Akm Humayun Kober, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Tomonori Nochi, Ayako Miyazaki, Hirohide Uenishi, Noriyuki Iwabuchi, Jin-Zhong Xiao, Julio Villena, Haruki Kitazawa

    Cells　8　(8)　2019/08/14

    DOI： 10.3390/cells8080891 　

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    Peptidoglycan recognition proteins (PGLYRPs) are a family of pattern recognition receptors (PRRs) that are able to induce innate immune responses through their binding to peptidoglycan (PGN), lipopolysaccharide, or lipoteichoic acid, or by interacting with other PRR-ligands. Recently, progress has been made in understanding the immunobiology of PGLYRPs in human and mice, however, their functions in livestock animals have been less explored. In this study, we characterized the expression patterns of PGLYRPs in porcine intestinal epithelial (PIE) cells and antigen-presenting cells (APCs) and their modulation by the interactions of host cells with PRR-ligands and non-viable immunomodulatory probiotics referred to as paraimmunobiotics. We demonstrated that PGLYRP-1, -2, -3, and -4 are expressed in PIE cells and APCs from Peyer's patches, being PGLYPR-3 and -4 levels higher than PGLYRP-1 and -2. We also showed that PGLYRPs expression in APCs and PIE cells can be modulated by different PRR agonists. By using knockdown PIE cells for TLR2, TLR4, NOD1, and NOD2, or the four PGLYRPs, we demonstrated that PGLYRPs expressions would be required for activation and functioning of TLR2, TLR4, NOD1, and NOD2 in porcine epitheliocytes, but PGLYRPs activation would be independent of those PRR expressions. Importantly, we reported for the first time that PGLYRPs expression can be differentially modulated by paraimmunobiotic bifidobacteria in a strain-dependent manner. These results provide evidence for the use of paraimmunobiotic bifidobacteria as an alternative for the improvement of resistance to intestinal infections or as therapeutic tools for the reduction of the severity of inflammatory damage in diseases in which a role of PGLYRPs-microbe interaction has been demonstrated.

  38. Comparative geochemical evaluation of toxic metals pollution and bacterial communities of industrial effluent tributary and a receiving estuary in Nigeria. International-journal Peer-reviewed

    Ganiyu O Oyetibo, Keisuke Miyauchi, Yi Huang, Wakako Ikeda-Ohtsubo, Mei-Fang Chien, Matthew O Ilori, Olukayode O Amund, Ginro Endo

    Chemosphere　227　638-646　2019/07

    DOI： 10.1016/j.chemosphere.2019.04.048 　

    ISSN： 0045-6535

  39. Isolation and Immunocharacterization of Lactobacillus salivarius from the Intestine of Wakame-Fed Pigs to Develop Novel "Immunosynbiotics". International-journal Peer-reviewed

    Yuki Masumizu, Binghui Zhou, A K M Humayun Kober, Md Aminul Islam, Hikaru Iida, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Leonardo Albarracin, Tomonori Nochi, Hisashi Aso, Keiichi Suzuki, Julio Villena, Haruki Kitazawa

    Microorganisms　7　(6)　2019/06/06

    DOI： 10.3390/microorganisms7060167 　

  40. Draft Genome Sequence of Weissella viridescens UCO-SMC3, Isolated from the Slime of Helix aspersa Müller Snails. International-journal Peer-reviewed

    Apolinaria Garcia-Cancino, Leonardo Albarracin, Marcela Espinoza-Monje, Jorge Campos-Martin, Valeria Garcia-Castillo, Yuka Nakano, Wakako Ikeda-Ohtsubo, Cristian Guitierrez-Zamorano, Hidetoshi Morita, Haruki Kitazawa, Julio Villena

    Microbiology resource announcements　8　(11)　2019/03/14

    DOI： 10.1128/MRA.01654-18 　

  41. Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation. International-journal Peer-reviewed

    Manami Igata, Md Aminul Islam, Asuka Tada, Michihiro Takagi, A K M Humayun Kober, Leonardo Albarracin, Hisashi Aso, Wakako Ikeda-Ohtsubo, Kenji Miyazawa, Kazutoyo Yoda, Fang He, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Frontiers in immunology　10　(MAY)　1180-1180　2019

    DOI： 10.3389/fimmu.2019.01180 　

  42. Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells. International-journal Peer-reviewed

    Paulraj Kanmani, Leonardo Albarracin, Hisakazu Kobayashi, Hikaru Iida, Ryoya Komatsu, A K M Humayun Kober, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Seiya Makino, Hiroshi Kano, Tadao Saito, Julio Villena, Haruki Kitazawa

    Molecular immunology　93　253-265　2018/01

    DOI： 10.1016/j.molimm.2017.07.009 　

    ISSN： 0161-5890

  43. Corrigendum: How Can We Define "Optimal Microbiota?": A Comparative Review of Structure and Functions of Microbiota of Animals, Fish, and Plants in Agriculture. International-journal Peer-reviewed

    Wakako Ikeda-Ohtsubo, Sylvia Brugman, Craig H Warden, Johanna M J Rebel, Gert Folkerts, Corné M J Pieterse

    Frontiers in nutrition　5　113-113　2018

    DOI： 10.3389/fnut.2018.00113 　

  44. Genomic Characterization of Lactobacillus delbrueckii TUA4408L and Evaluation of the Antiviral Activities of its Extracellular Polysaccharides in Porcine Intestinal Epithelial Cells. International-journal Peer-reviewed

    Paulraj Kanmani, Leonardo Albarracin, Hisakazu Kobayashi, Elvira Maria Hebert, Lucila Saavedra, Ryoya Komatsu, Brian Gatica, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Shintaro Egusa, Takashi Mishima, Alexis Salas-Burgos, Hideki Takahashi, Julio Villena, Haruki Kitazawa

    Frontiers in immunology　9　(SEP)　2178-2178　2018

    DOI： 10.3389/fimmu.2018.02178 　

    ISSN： 1664-3224

  45. A Comparative Review on Microbiota Manipulation: Lessons From Fish, Plants, Livestock, and Human Research. International-journal Peer-reviewed

    Sylvia Brugman, Wakako Ikeda-Ohtsubo, Saskia Braber, Gert Folkerts, Corné M J Pieterse, Peter A H M Bakker

    Frontiers in nutrition　5　80-80　2018

    Publisher: Frontiers Media {SA}

    DOI： 10.3389/fnut.2018.00080 　

    ISSN： 2296-861X

  46. Ecophysiology of sulfate-reducing bacteria (SRB) in the intestinal tract

    大坪和香子

    無菌生物　47　(1)　30-38　2017/09

    Publisher: 日本無菌生物ノートバイオロジー学会

    ISSN： 0910-0903

  47. Biodegradation of crude oil and phenanthrene by heavy metal resistant Bacillus subtilis isolated from a multi-polluted industrial wastewater creek Peer-reviewed

    Ganiyu Oladunjoye Oyetibo, Mei-Fang Chien, Wakako Ikeda-Ohtsubo, Hitoshi Suzuki, Oluwafemi Sunday Obayori, Sunday Adekunle Adebusoye, Matthew Olusoji Ilori, Olukayode Oladipo Amund, Ginro Endo

    International Biodeterioration & Biodegradation　120　143-151　2017/05

    DOI： 10.1016/j.ibiod.2017.02.021 　

    ISSN： 0964-8305

    eISSN： 1879-0208

  48. Draft Genome Sequence of Lactobacillus plantarum MPL16, a Wakame-Utilizing Immunobiotic Strain Isolated from Swine Feces. International-journal Peer-reviewed

    Julio Villena, Lucila Saavedra, Elvira Maria Hebert, Yoshihito Suda, Yuki Masumizu, Leonardo Albarracin, Patricia Clua, Wakako Ikeda-Ohtsubo, Haruki Kitazawa

    Genome announcements　5　(10)　e00006-e00017　2017/03/09

    Publisher: American Society for Microbiology

    DOI： 10.1128/genomeA.00006-17 　

    ISSN： 2169-8287

  49. Draft Genome Sequence of the Immunobiotic Strain Lactobacillus jensenii TL2937. International-journal Peer-reviewed

    Julio Villena, Yuki Masumizu, Hikaru Iida, Wakako Ikeda-Ohtsubo, Leonardo Albarracin, Seiya Makino, Sou Ohkawara, Katsunori Kimura, Lucila Saavedra, Elvira Maria Hebert, Haruki Kitazawa

    Genome announcements　5　(9)　2017/03/02

    Publisher: American Society for Microbiology

    DOI： 10.1128/genomeA.00005-17 　

    ISSN： 2169-8287

  50. ビフィズス菌のクロスフィーディングを利用した炎症性腸疾患を予防するプロバイオティクスの開発

    大坪 和香子, 齋藤 忠夫

    メディカル・サイエンス・ダイジェスト　43　2-4　2017

  51. Draft Genome Sequence of Lactobacillus plantarum TL2766, a Strain with the Ability To Ferment Wakame. International-journal Peer-reviewed

    Julio Villena, Lucila Saavedra, Elvira Maria Hebert, Yuki Masumizu, Nana Sato, A K M Humayun Kober, Leonardo Albarracin, Wakako Ikeda-Ohtsubo, Seiya Makino, Katsunori Kimura, Sou Ohkawara, Haruki Kitazawa

    Genome announcements　4　(6)　2016/11/23

    Publisher: American Society for Microbiology

    DOI： 10.1128/genomeA.01328-16 　

    ISSN： 2169-8287

  52. Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937 Peer-reviewed

    Kobayashi, H., Albarracin, L., Sato, N., Kanmani, P., Kober, A.K.M.H., Ikeda-Ohtsubo, W., Suda, Y., Nochi, T., Aso, H., Makino, S., Kano, H., Ohkawara, S., Saito, T., Villena, J., Kitazawa, H.

    Beneficial Microbes　7　(5)　769-782　2016

    DOI： 10.3920/BM2016.0095 　

    ISSN： 1876-2883

    eISSN： 1876-2891

  53. Mercury bioremoval by Yarrowia strains isolated from sediments of mercury-polluted estuarine water. International-journal Peer-reviewed

    Ganiyu Oladunjoye Oyetibo, Shakirat Titilayo Ishola, Wakako Ikeda-Ohtsubo, Keisuke Miyauchi, Matthew Olusoji Ilori, Ginro Endo

    Applied microbiology and biotechnology　99　(8)　3651-7　2015/04

    DOI： 10.1007/s00253-014-6279-1 　

    ISSN： 0175-7598

  54. Reducing environmental impact in composting of solid wastes from livestock wastewater treatment system

    大坪和香子, 進藤絵里香, 山田剛史, 上田英代, 上田裕一, 渡辺昭, 宮内啓介, 遠藤銀朗

    Journal of Environmental Biotechnology　15　(1)　17-22　2015

    Publisher: 環境バイオテクノロジー学会

    ISSN： 2436-5041

  55. Structural and functional analysis of a novel haloalkane dehalogenase with two halide-binding sites. International-journal Peer-reviewed

    Radka Chaloupkova, Tatyana Prudnikova, Pavlina Rezacova, Zbynek Prokop, Tana Koudelakova, Lukas Daniel, Jan Brezovsky, Wakako Ikeda-Ohtsubo, Yukari Sato, Michal Kuty, Yuji Nagata, Ivana Kuta Smatanova, Jiri Damborsky

    Acta crystallographica. Section D, Biological crystallography　70　(Pt 7)　1884-97　2014/07

    DOI： 10.1107/S1399004714009018 　

    ISSN： 0907-4449

  56. Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor. Peer-reviewed

    Wakako Ikeda-Ohtsubo, Morio Miyahara, Takeshi Yamada, Akira Watanabe, Shinya Fushinobu, Takayoshi Wakagi, Hirofumi Shoun, Keisuke Miyauchi, Ginro Endo

    Journal of bioscience and bioengineering　116　(6)　722-4　2013/12

    DOI： 10.1016/j.jbiosc.2013.05.024 　

    ISSN： 1389-1723

  57. Community structure and population dynamics of ammonia oxidizers in composting processes of ammonia-rich livestock waste. International-journal Peer-reviewed

    Takeshi Yamada, Shinya Araki, Wakako Ikeda-Ohtsubo, Keiko Okamura, Akira Hiraishi, Hideyo Ueda, Yasuichi Ueda, Keisuke Miyauchi, Ginro Endo

    Systematic and applied microbiology　36　(5)　359-67　2013/07

    DOI： 10.1016/j.syapm.2013.02.001 　

    ISSN： 0723-2020

  58. Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2. Peer-reviewed

    Wakako Ikeda-Ohtsubo, Morio Miyahara, Sang-Wan Kim, Takeshi Yamada, Masaki Matsuoka, Akira Watanabe, Shinya Fushinobu, Takayoshi Wakagi, Hirofumi Shoun, Keisuke Miyauchi, Ginro Endo

    Journal of bioscience and bioengineering　115　(1)　37-42　2013/01

    DOI： 10.1016/j.jbiosc.2012.08.015 　

    ISSN： 1389-1723

  59. 'Candidatus Ancillula trichonymphae', a novel lineage of endosymbiotic Actinobacteria in termite gut flagellates of the genus Trichonympha. International-journal Peer-reviewed

    Jürgen F H Strassert, Tim Köhler, Tobias H G Wienemann, Wakako Ikeda-Ohtsubo, Nicolas Faivre, Sibylle Franckenberg, Rudy Plarre, Renate Radek, Andreas Brune

    Environmental microbiology　14　(12)　3259-70　2012/12

    DOI： 10.1111/1462-2920.12012 　

    ISSN： 1462-2912

  60. Complete genome sequence of Bradyrhizobium sp. S23321: insights into symbiosis evolution in soil oligotrophs. Peer-reviewed

    Takashi Okubo, Takahiro Tsukui, Hiroko Maita, Shinobu Okamoto, Kenshiro Oshima, Takatomo Fujisawa, Akihiro Saito, Hiroyuki Futamata, Reiko Hattori, Yumi Shimomura, Shin Haruta, Sho Morimoto, Yong Wang, Yoriko Sakai, Masahira Hattori, Shin-Ichi Aizawa, Kenji V P Nagashima, Sachiko Masuda, Tsutomu Hattori, Akifumi Yamashita, Zhihua Bao, Masahito Hayatsu, Hiromi Kajiya-Kanegae, Ikuo Yoshinaga, Kazunori Sakamoto, Koki Toyota, Mitsuteru Nakao, Mitsuyo Kohara, Mizue Anda, Rieko Niwa, Park Jung-Hwan, Reiko Sameshima-Saito, Shin-Ichi Tokuda, Sumiko Yamamoto, Syuji Yamamoto, Tadashi Yokoyama, Tomoko Akutsu, Yasukazu Nakamura, Yuka Nakahira-Yanaka, Yuko Takada Hoshino, Hideki Hirakawa, Hisayuki Mitsui, Kimihiro Terasawa, Manabu Itakura, Shusei Sato, Wakako Ikeda-Ohtsubo, Natsuko Sakakura, Eli Kaminuma, Kiwamu Minamisawa

    Microbes and environments　27　(3)　306-15　2012

    DOI： 10.1264/jsme2.ME11321 　

    ISSN： 1342-6311

    eISSN： 1347-4405

  61. Survival of the aerobic denitrifier Pseudomonas stutzeri strain TR2 during co-culture with activated sludge under denitrifying conditions. International-journal Peer-reviewed

    Morio Miyahara, Sang-Wan Kim, Shengmin Zhou, Shinya Fushinobu, Takeshi Yamada, Wakako Ikeda-Ohtsubo, Akira Watanabe, Keisuke Miyauchi, Ginro Endo, Takayoshi Wakagi, Hirofumi Shoun

    Bioscience, biotechnology, and biochemistry　76　(3)　495-500　2012

    DOI： 10.1271/bbb.110785 　

    ISSN： 0916-8451

    eISSN： 1347-6947

  62. コンポスト製造過程において発生する亜酸化窒素の削減に関与する脱窒細菌遺伝子の多様性と消長 Peer-reviewed

    進藤絵里香, 大坪和香子, 上田裕一, 上田英代, 宮内啓介, 遠藤銀朗

    土木学会論文集G(環境)　Vol.67, NO.7　441-448　2011

  63. Putatively free-living 'Endomicrobia'- ancestors of the intracellular symbionts of termite gut flagellates? International-journal Peer-reviewed

    Wakako Ikeda-Ohtsubo, Nicolas Faivre, Andreas Brune

    Environmental microbiology reports　2　(4)　554-9　2010/08

    DOI： 10.1111/j.1758-2229.2009.00124.x 　

    ISSN： 1758-2229

  64. Strict cospeciation of devescovinid flagellates and Bacteroidales ectosymbionts in the gut of dry-wood termites (Kalotermitidae). International-journal Peer-reviewed

    Mahesh S Desai, Jürgen F H Strassert, Katja Meuser, Horst Hertel, Wakako Ikeda-Ohtsubo, Renate Radek, Andreas Brune

    Environmental microbiology　12　(8)　2120-32　2010/08

    DOI： 10.1111/j.1462-2920.2009.02080.x 　

    ISSN： 1462-2912

  65. ゲノム解析ソフトウエアGenomeMatcher

    大坪嘉行, 大坪和香子, 永田裕二, 津田雅孝

    化学と生物　48　(5)　313-319　2010/05/01

    Publisher: 公益社団法人 日本農芸化学会

    DOI： 10.1271/kagakutoseibutsu.48.313 　

    ISSN： 0453-073X

    eISSN： 1883-6852

    More details Close

    ゲノム情報のような多量の情報を効率良く取り扱うには，個々の研究者が情報処理技術を有していることが望ましい．しかし，多くのいわゆるウエットな研究者は，プログラミングやコマンドラインツールの取り扱いを苦手としており，グラフィカルなユーザーインターフェースを備えたソフトウエアが必要である．ここでは，ゲノム解析関連だけでなく情報処理に有用な機能を備えたソフトウエア，GenomeMatcherを紹介する．

  66. Cospeciation of termite gut flagellates and their bacterial endosymbionts: Trichonympha species and 'Candidatus Endomicrobium trichonymphae'. International-journal Peer-reviewed

    Wakako Ikeda-Ohtsubo, Andreas Brune

    Molecular ecology　18　(2)　332-42　2009/01

    DOI： 10.1111/j.1365-294X.2008.04029.x 　

    ISSN： 0962-1083

    eISSN： 1365-294X

  67. Genomic analysis of "Elusimicrobium minutum" the first cultivated representative of the phylum "Elusimicrobia" (formerly termite group 1) Peer-reviewed

    Herlemann, D.P.R., Geissinger, O., Ikeda-Ohtsubo, W., Kunin, V., Sun, H., Lapidus, A., Hugenholtz, P., Brune, A.

    Applied and Environmental Microbiology　75　(9)　2841-2849　2009

    DOI： 10.1128/AEM.02698-08 　

    ISSN： 0099-2240

  68. GenomeMatcher: a graphical user interface for DNA sequence comparison. International-journal Peer-reviewed

    Yoshiyuki Ohtsubo, Wakako Ikeda-Ohtsubo, Yuji Nagata, Masataka Tsuda

    BMC bioinformatics　9　(1)　376-376　2008/09/16

    DOI： 10.1186/1471-2105-9-376 　

    ISSN： 1471-2105

  69. Phylogenetic diversity of 'Endomicrobia' and their specific affiliation with termite gut flagellates. International-journal Peer-reviewed

    Wakako Ikeda-Ohtsubo, Mahesh Desai, Ulrich Stingl, Andreas Brune

    Microbiology (Reading, England)　153　(Pt 10)　3458-3465　2007/10

    DOI： 10.1099/mic.0.2007/009217-0 　

    ISSN： 1350-0872

Show all ︎Show first 5

Misc. 67

  1. 腸-肺軸を介する「ポストイムノバイオティクス」の抗ウイルス免疫機能評価

    福山皓太朗, 福山皓太朗, 生井楓, 生井楓, 大坪和香子, 大坪和香子, 西山啓太, 西山啓太, VILLENA Julio, VILLENA Julio, 北澤春樹, 北澤春樹

    日本畜産学会大会講演要旨　132nd　2024

    ISSN： 1342-4688

  2. 乳酸菌によるシアロオリゴ糖代謝から見た子豚の腸内細菌叢形成

    橋本凌河, 西山啓太, 生井楓, 鈴木香澄, 鈴木香澄, 福田伊津子, 杉山友太, 大坪和香子, 吉岡豪, 中崎瑛里, 北澤春樹

    ミルクサイエンス(Web)　73　(2)　2024

    ISSN： 2188-0700

  3. Development of piglet gut microbiota through sialo-oligosaccharide metabolism by lactic acid bacteria.

    橋本凌河, 西山啓太, 生井楓, 鈴木香澄, 鈴木香澄, 福田伊津子, 杉山友太, 豊吉瑛太, 佐久間大河, 大坪和香子, 吉岡豪, 中崎瑛里, 北澤春樹

    日本乳酸菌学会誌　35　(2)　2024

    ISSN： 1343-327X

  4. How female researchers overcome challenges to conduct research abroad

    大坪和香子

    日本農芸化学会大会講演要旨集(Web)　2023　2023

    ISSN： 2186-7976

  5. Regulation of gut starvation response through Drosophila NP3253 neurons

    NGUYEN Quang-Dat, 藤井希帆, 石橋謙太朗, 布施直之, 大坪和香子, 北澤春樹, 谷本拓, 倉田祥一朗

    日本分子生物学会年会プログラム・要旨集(Web)　46th　2023

  6. Regulation of starvation response through neural control in Drosophila

    藤井希帆, NGUYEN Quang-Dat, 石橋謙太朗, 布施直之, 谷本拓, 北澤春樹, 大坪和香子, 倉田祥一朗

    日本分子生物学会年会プログラム・要旨集(Web)　46th　2023

  7. 青色光毒性を用いたDrosophila melanogasterの人為選抜による脂質代謝関連形質および腸内細菌叢の変化

    高田悠太, 大坪和香子, 堀雅敏

    日本応用動物昆虫学会大会講演要旨　67th　2023

  8. Acetobacter symbionts contribute to blue light tolerance in Drosophila

    高田悠太, 大坪和香子, 布施直之, 堀雅敏

    日本微生物生態学会大会(Web)　36th　2023

  9. ブタ腸管上皮細胞においてミトコンドリア機能を改善するポストイムノバイオティクスの探索

    生井楓, 生井楓, 鈴木眞依, 笠原日菜子, 西山啓太, 西山啓太, 大坪和香子, 大坪和香子, 北澤春樹, 北澤春樹

    日本畜産学会大会講演要旨　131st　2023

    ISSN： 1342-4688

  10. 乳腺上皮細胞における乳成分の炎症制御に関する基礎的研究

    福山皓太朗, 生井楓, 生井楓, 周冰卉, 周冰卉, 大坪和香子, 大坪和香子, 西山啓太, 西山啓太, 麻生久, 麻生久, 北澤春樹, 北澤春樹

    ミルクサイエンス(Web)　72　(2)　2023

    ISSN： 2188-0700

  11. 新規抗ウイルス性「ポストイムノバイオティクス」の選抜・機構解明

    今村圭哉, 今村圭哉, 荒木祥多, 荒木祥多, 生井楓, 生井楓, 宮崎綾子, 須田遊人, 大坪和香子, 大坪和香子, 西山啓太, 西山啓太, 北澤春樹, 北澤春樹

    日本畜産学会大会講演要旨　131st　2023

    ISSN： 1342-4688

  12. ウマ腸内細菌Streptococcus equinusによるイヌリンとセロビオースの菌株依存的資化性

    大坪和香子, 西村順子, 神山結斗, 周冰卉, 西山啓太, 北澤春樹

    日本ウマ科学会学術集会講演要旨集　35th　2022

  13. ロタウイルス感染低減性イムノバイオティクスの選抜及び評価

    荒木祥多, 北原秀悟, 扇隆介, 櫻井美月, 周冰卉, 周冰卉, 庄涛, 宮崎綾子, 宮澤光太郎, 大坪和香子, 大坪和香子, 麻生久, 麻生久, 北澤春樹, 北澤春樹

    ミルクサイエンス(Web)　71　(2)　2022

    ISSN： 2188-0700

  14. Evaluation of applicability of horse manure compost for development of sustainable agriculture in Fukushima coastal area: Analysis of microbial microbiota and chemical components of horse manure compost

    NISHIMURA Junko, IKEDA-OHTSUBO Wakako

    Journal of Reconstruction Agriculture and Sciences　1　(2)　1-11　2021/07/30

    Publisher: Society of Reconstruction Agriculture

    DOI： 10.57341/jras.1.2_1 　

    eISSN： 2758-1160

    More details Close

    Development of locally-available microbial materials is a high-priority issue for future food production in sustainable agricultural management. In this study, we focused on the horticultural value of horse manure from retired racehorses reared at a local stable in Minami-soma City as a potential composting material applicable for sustainable agriculture in Fukushima Prefecture and performed a bench-top composting experiment. The maturation of the resulting compost was confirmed by the increase of nutritive elements including P, N and K as well as the final C/N ratio of 27.4, which is in the range of the ideal values of mature composts. Microbial composition before and after composting was investigated by 16S rRNA gene sequencing, which showed that the starting material (horse manure) was dominated by bacterial groups affiliated with obligate anaerobic bacterial taxa from equine cecal microbiota , while the mature compost was dominated by bacterial groups of many aerobic or facultative anaerobic taxa, which were likely to be involved in aerobic degradation of organic substrates during the ripening process, indicating that the microbial composition changed significantly during the ripening period. In addition, the germination rate and oxygen consumption after ripening met the generally approved standard, which emphasized that horse manure from retired racehorses can serve as a good microbial material for producing high-quality composts in a space- and time-efficient way compared to other livestock manures. By reference to these results, we plan to build a local platform to produce horse manure composts with scientifically proven values toward the practical implementation.

  15. Advanced in vitro evaluation to explore novel anti-rotavirus immunobiotics

    北原秀悟, 荒木祥多, 扇隆介, 櫻井美月, 周冰卉, 周冰卉, 庄涛, 宮崎綾子, 宮澤光太郎, 大坪和香子, 大坪和香子, 麻生久, 麻生久, 北澤春樹, 北澤春樹

    日本畜産学会大会講演要旨　129th　2021

    ISSN： 1342-4688

  16. 豚小腸由来の抗ウイルスイムノバイオティクス(Anti-viral immunobiotics from porcine small intestine)

    犬童 優樹, Zhou Binghui, Islam Md. Aminul, 宮崎 綾子, 大坪 和香子, 須田 義人, 麻生 久, Villena Julio, 北澤 春樹

    日本畜産学会大会講演要旨集　126回　85-85　2019/09

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  17. 粘膜結合因子はLactobacillus rhamnosustoの腸管粘膜への接着能を制御する(Mucus-binding factor regulate the adhesion ability of Lactobacillus rhamnosusto intestinal mucosa)

    友常 加惠, 水野 滉也, 周 冰卉, 犬童 優樹, Villena Julio, Albarracin Leonardo, Islam Md. Aminul, 西山 啓太, 大坪 和香子, 北澤 春樹

    日本畜産学会大会講演要旨集　126回　86-86　2019/09

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  18. 炎症性腸疾患in vitroモデル系によるイムノバイオティック評価

    湯澤 真央, 佐藤 菜, 小松 稜弥, 高木 理宏, 大坪 和香子, 麻生 久, 北澤 春樹

    ミルクサイエンス　68　(2)　148-148　2019/08

    Publisher: 日本酪農科学会

    ISSN： 1343-0289

    eISSN： 2188-0700

  19. イムノバイオティックLactobacillus rhamnosusのMBFノックアウト株の樹立

    友常 加惠, 水野 滉也, 周 冰卉, Albarracin Leonardo, Islam Md Aminul, 西山 啓太, 大坪 和香子, Villena Julio, 佐々木 泰子, 北澤 春樹

    ミルクサイエンス　68　(2)　148-148　2019/08

    Publisher: 日本酪農科学会

    ISSN： 1343-0289

    eISSN： 2188-0700

  20. 新規イムノバイオティクス開発のための酪農乳酸菌の検出と同定

    土田 咲絵, 大坪 和香子, 須田 義人, 北澤 春樹

    ミルクサイエンス　68　(2)　150-150　2019/08

    Publisher: 日本酪農科学会

    ISSN： 1343-0289

    eISSN： 2188-0700

  21. オキナワモズクフコイダンのプレバイオティクス効果の研究

    大坪 和香子, 有川 優希, 鈴木 錠, 田村 彩佳, 北澤 春樹

    ミルクサイエンス　68　(2)　150-150　2019/08

    Publisher: 日本酪農科学会

    ISSN： 1343-0289

    eISSN： 2188-0700

  22. 抗下痢イムノバイオティクスを選択するin vitro評価システムの進歩(Advanced in vitro evaluation system for selecting anti-diarrheal immunobiotics)

    小松 稜弥, 飯田 ひかる, Albarracn Leonardo, Islam Aminul Md., 大坪 和香子, 麻生 久, 岩渕 紀介, 清水 金忠[肖], 須田 義人, 齋藤 忠夫, Villena Julio, 北澤 春樹

    日本畜産学会大会講演要旨集　125回　121-121　2019/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  23. 豚消化管ムチンと上皮細胞に付着するワカメ資化性乳酸菌の選択(Selection of wakame utilizing lactobacilli adhering to porcine intestinal mucin and epitheliocytes)

    周 冰卉, 舛水 由季, 中野 祐香, 犬童 優樹, 小松 稜弥, Islam Md. Aminul, 大坪 和香子, 須田 義人, 鈴木 啓一, 麻生 久, 北澤 春樹

    日本畜産学会大会講演要旨集　125回　199-199　2019/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  24. 乳房炎病原菌にて刺激した牛乳腺上皮細胞のマイクロアレイ解析(Microarray analysis of bovine mammary epithelial cells stimulated with mastitis pathogens)

    高木 理宏, 小松 稜弥, Villena Julio, Albarracin Leonardo, Islam Md. Aminul, 須田 義人, 大坪 和香子, 野地 智法, 麻生 久, 北澤 春樹

    日本畜産学会大会講演要旨集　125回　200-200　2019/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  25. Streptococcus thermophilusの菌体外多糖類はウイルス感染防御能をもつ(Exopolysaccharides of Streptococcus thermophilus has potentials against virus infection)

    水野 滉也, 佐々木 泰子, 友常 加恵, 犬童 優樹, Albarracin Leonardo, Islam Md. Aminul, 大坪 和香子, 牧野 聖也, 麻生 久, Villena Julio, 北澤 春樹

    日本畜産学会大会講演要旨集　125回　200-200　2019/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  26. 豚イムノバイオティクスライブラリーの構築(Construction of porcine immunobiotics library)

    中野 祐香, 舛水 由季, 扇 隆介, Albarracin Leonardo, 大坪 和香子, Islam Md Aminul, 須田 義人, 麻生 久, Villena Julio, 北澤 春樹

    日本畜産学会大会講演要旨集　125回　200-200　2019/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  27. イムノバイオティックLactobacillus rhamnosusのMBFノックアウト株の樹立

    友常加惠, 友常加惠, 友常加惠, 水野滉也, 水野滉也, 水野滉也, 周冰卉, 周冰卉, 周冰卉, ALBARRACIN Leonardo, ISLAM Md Aminul, ISLAM Md Aminul, ISLAM Md Aminul, 西山啓太, 大坪和香子, 大坪和香子, 大坪和香子, VILLENA Julio, 佐々木泰子, 北澤春樹, 北澤春樹, 北澤春樹

    ミルクサイエンス(Web)　68　(2)　2019

    ISSN： 2188-0700

  28. ヒト腸管粘膜中に常在する硫酸還元細菌の研究

    日下 練介, 大坪 和香子, 齋藤 忠夫, 北澤 春樹

    無菌生物　48　(2)　68-69　2018/12

    Publisher: 日本無菌生物ノートバイオロジー学会

    ISSN： 0910-0903

  29. ハチミツに含まれるオリゴ糖の特徴とプレバイオティクス効果

    澤田 侑希, 大坪 和香子, 齋藤 忠夫, 北澤 春樹

    無菌生物　48　(2)　73-74　2018/12

    Publisher: 日本無菌生物ノートバイオロジー学会

    ISSN： 0910-0903

  30. Lactobacillus bulgaricusのラクトース取込みの対向輸送解析と低ガラクトースヨーグルトの開発

    山田滉介, 大坪和香子, 北澤春樹, 北澤春樹, 齋藤忠夫

    日本畜産学会大会講演要旨　124回　155-155　2018/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  31. 日本人腸内フローラによるオキナワモズクフコイダン分解メカニズムの解明

    大坪 和香子

    奨励研究報告書 / 東和食品研究振興会 編　91-98　2018

    Publisher: 東京 : 東和食品研究振興会

  32. Identification of sulfate-reducing bacteria in human intestinal mucus

    大坪和香子

    無菌生物　47　(1)　57-58　2017/09

    Publisher: 日本無菌生物ノートバイオロジー学会

    ISSN： 0910-0903

  33. ヒト腸管粘膜中に常在する硫酸還元細菌の検出と同定

    大坪 和香子, 日下 練介, 北澤 春樹, 齋藤 忠夫

    無菌生物　47　(1)　57-58　2017/09

    Publisher: 日本無菌生物ノートバイオロジー学会

    ISSN： 0910-0903

  34. PIP細胞におけるTLRを介する脂質/免疫関連因子のトランスクリプトーム解析

    井形 愛美, Kober A.K.M. Humayun, 多田 明日翔, Albarracin Leonardo, 大坪 和香子, 麻生 久, 宮澤 賢司, 依田 一豊, 何 方, 齋藤 忠夫, Villena Julio, 北澤 春樹

    ミルクサイエンス　66　(2)　167-167　2017/08

    Publisher: 日本酪農科学会

    ISSN： 1343-0289

  35. Lactobacillus bulgaricusのラクトース取込みの対向輸送解析と低ガラクトースヨーグルトの開発

    山田 滉介, 大坪 和香子, 北澤 春樹, 齋藤 忠夫

    ミルクサイエンス　66　(2)　168-168　2017/08

    Publisher: 日本酪農科学会

    ISSN： 1343-0289

  36. 発酵乳に利用されるビフィズス菌のハチミツオリゴ糖資化性の検討

    澤田 侑希, 大坪 和香子, 北澤 春樹, 齋藤 忠夫

    ミルクサイエンス　66　(2)　172-172　2017/08

    Publisher: 日本酪農科学会

    ISSN： 1343-0289

    eISSN： 2188-0700

  37. 炎症性腸疾患を悪化させる硫酸基還元細菌に対するビフィズス菌の付着阻害作用の研究

    日下 練介, 大坪 和香子, 北澤 春樹, 齋藤 忠夫

    ミルクサイエンス　66　(2)　173-173　2017/08

    Publisher: 日本酪農科学会

    ISSN： 1343-0289

    eISSN： 2188-0700

  38. ブタ腸管におけるペプチドグリカン認識タンパク質の発現とその調節機構

    飯田ひかる, 飯田ひかる, 遠野雅徳, 須田義人, 大坪和香子, 大坪和香子, 齋藤忠夫, JULIO Villena, JULIO Villena, 北澤春樹, 北澤春樹

    日本畜産学会大会講演要旨　122nd　104　2017/03/28

    ISSN： 1342-4688

  39. ブタ腸管由来ワカメ資化性乳酸菌のイムノバイオティック利用性

    舛水由季, 飯田ひかる, 須田義人, 大坪和香子, 麻生久, 齋藤忠夫, 鈴木啓一, 北澤春樹

    日本畜産学会大会講演要旨　122nd　198　2017/03/28

    ISSN： 1342-4688

  40. 炎症性腸疾患を悪化させる硫酸基還元細菌に対するビフィズス菌の付着阻害作用の研究

    日下練介, 大坪和香子, 北澤春樹, 齋藤忠夫

    日本畜産学会大会講演要旨　122nd　199　2017/03/28

    ISSN： 1342-4688

  41. 高シュウ酸植物を摂食するハムシ腸内フローラの特性解析

    大坪和香子, 宮城敦子, LEE Michael, Kwan Fu, 森宙史, 黒川顕, 小島紀幸

    日本農芸化学会大会講演要旨集(Web)　2017　ROMBUNNO.2C19a01 (WEB ONLY)　2017/03/05

    ISSN： 2186-7976

  42. ブタ腸管におけるペプチドグリカン認識タンパク質の発現とその調節機構

    飯田 ひかる, 遠野 雅徳, 須田 義人, 大坪 和香子, 齋藤 忠夫, Julio Villena, 北澤 春樹

    日本畜産学会大会講演要旨集　122回　104-104　2017/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  43. ブタ腸管由来ワカメ資化性乳酸菌のイムノバイオティック利用性

    舛水 由季, 飯田 ひかる, 須田 義人, 大坪 和香子, 麻生 久, 齋藤 忠夫, 鈴木 啓一, 北澤 春樹

    日本畜産学会大会講演要旨集　122回　198-198　2017/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  44. 炎症性腸疾患を悪化させる硫酸基還元細菌に対するビフィズス菌の付着阻害作用の研究

    日下 練介, 大坪 和香子, 北澤 春樹, 齋藤 忠夫

    日本畜産学会大会講演要旨集　122回　199-199　2017/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  45. シロアリ腸内のスピロヘータと仲間たち~木質多糖の嫌気発酵を中心とする進化型微生物生態系~

    大坪和香子

    レプトスピラ・シンポジウム　54th　7　2017

  46. シロアリ腸内フローラ研究から見た腸内共生系の進化と機能

    大坪和香子

    無菌生物　46　(1)　34(JA),35(EN)　2016/09/01

    ISSN： 0910-0903

  47. ブタにおけるペプチドグリカン認識タンパク質ファミリーの発現とその調節

    飯田ひかる, 遠野雅徳, 須田義人, 麻生久, 大坪和香子, 齋藤忠夫, 北澤春樹

    日本畜産学会大会講演要旨　120th　104　2015/09/11

    ISSN： 1342-4688

  48. 畜産廃水処理システム由来の固形廃棄物の堆肥化における環境負荷低減化

    大坪和香子, 上田英代, 上田裕一, 渡辺昭, 宮内啓介, 遠藤銀朗

    日本農芸化学会大会講演要旨集(Web)　2015　4SY22-3 (WEB ONLY)　2015/03/05

    ISSN： 2186-7976

  49. エゾノギシギシを摂食するGastrophysa属ハムシ腸内フローラの地域的差異

    大坪和香子, 宮城敦子, 小島紀幸, 川合真紀, 内宮博文, 宮内啓介, 遠藤銀朗

    日本農芸化学会大会講演要旨集(Web)　2015　4A34A15 (WEB ONLY)　2015/03/05

    ISSN： 2186-7976

  50. ミクロな動物園 : 腸内微生物の豊かな個性

    大坪 和香子

    東北学院大学工学総合研究所紀要 Bulletin of Research Institute for Engineering and Technology, Tohoku Gakuin University　(4)　329-373　2015/03

    Publisher: 東北学院大学工学総合研究所

  51. VOL.16 大坪和香子(スポットライト : イケメン&イケジョ)

    大坪 和香子

    日本微生物生態学会誌　30　(1)　29-30　2015

    Publisher: 日本微生物生態学会

    DOI： 10.20709/jsmeja.30.1_29 　

    eISSN： 2424-1989

  52. 6th KJT symposium報告(国際学会見聞録)

    村瀬 潤, 大坪 和香子, 鈴木 研志

    日本微生物生態学会誌　30　(1)　33-33　2015

    Publisher: 日本微生物生態学会

    DOI： 10.20709/jsmeja.30.1_33 　

    eISSN： 2424-1989

  53. O13-04 酸素存在下において亜酸化窒素(N_2O)発生を抑制する脱窒細菌の単離と特性解析(口頭発表)

    大坪 和香子, 佐賀 武, 鈴木 拓磨, 宮内 啓介, 遠藤 銀朗

    日本微生物生態学会講演要旨集　2014　90-90　2014

    Publisher: 日本微生物生態学会

  54. 国際的な研究キャリアパスを築く : 留学&ライフステージのハードル・メリット・デメリット(2014年度環境微生物系合同大会ランチョンシンポジウム)

    濱村 奈津子, 鮫島 玲子, 大坪 和香子, 三宅 恵子

    日本微生物生態学会講演要旨集　2014　272-273　2014

    Publisher: 日本微生物生態学会

  55. 堆肥化プロセスにおける亜酸化窒素発生に硝化・脱窒細菌が与える影響

    大坪和香子, 進藤絵里香, 上田英代, 上田裕一, 宮内啓介, 遠藤銀朗

    日本農芸化学会大会講演要旨集(Web)　2013　4C11A08 (WEB ONLY)　2013/03/05

    ISSN： 2186-7976

  56. 好気的条件下で脱窒を行う能力を有する堆肥由来細菌の単離と同定

    大坪和香子, 尾形裕明, 宮内啓介, 遠藤銀朗

    日本農芸化学会大会講演要旨集(Web)　2012　2C22A10 (WEB ONLY)　2012/03/05

    ISSN： 2186-7976

  57. 畜産廃棄物のコンポスト化プロセスに存在する脱窒細菌の群集構造の変化

    進藤 絵里香, 大坪 和香子, 宮内 啓介

    東北学院大学工学総合研究所紀要 Bulletin of Research Institute for Engineering and Technology, Tohoku Gakuin University　(1)　47-56　2012/03

    Publisher: 東北学院大学工学総合研究所

  58. PE-21 Effect of mismatches in PCR primers for amplifying nitrous oxide reductase (nosZ) genes on community structure analysis of denitrifying bacteria(PE Microbial community dynamics,Poster session)

    Ikeda-Ohtsubo Wakako, Miyauchi Keisuke, Endo Ginro

    日本微生物生態学会講演要旨集　(28)　156-156　2012

    Publisher: 日本微生物生態学会

  59. 耐酸素性脱窒細菌Pseudomonas stutzeri TR2株の脱窒条件における優勢化へ電子受容体が与える影響の評価

    大坪和香子, 白取早恵, 宮内啓介, 遠藤銀朗

    環境バイオテクノロジー学会大会プログラム講演要旨集　44th　49　2011/06/20

  60. コンポスト製造過程において発生する亜酸化窒素の削減に関与する脱窒細菌遺伝子の多様性と消長—Diversity and succession dynamics of bacterial genes relating to the reduction of nitrous oxide (N₂O) emitted from composting processes

    進藤 絵里香, 大坪 和香子, 上田 裕一, 上田 英代, 宮内 啓介, 遠藤 銀朗

    環境工学研究論文集 = Environmental research / 土木学会環境工学委員会 編　48　Ⅲ_441-448　2011

    Publisher: 東京 : 土木学会

    ISSN： 1341-5115

  61. 101p-8 養豚場排水処理残渣の堆肥化プロセスに存在する脱窒細菌群集の定量および系統解析(101p 水圏生態系/群集構造解析,口頭発表)

    大坪 和香子, 進藤 絵里香, 上田 英代, 上田 裕一, 宮内 啓介, 遠藤 銀朗

    日本微生物生態学会講演要旨集　(27)　50-50　2011

    Publisher: 日本微生物生態学会

  62. 亜酸化窒素発生抑止を目的とした特殊脱窒細菌のバイオオーグメンテーションの試み

    大坪和香子, 宮原盛雄, 渡辺昭, 宮内啓介, 若木高善, 祥雲弘文, 遠藤銀朗

    日本農芸化学会大会講演要旨集　2010　251　2010/03/05

  63. 畜産廃棄物のコンポスト製造過程に関与するアンモニア酸化細菌群の多様性と遷移に関する研究

    荒木伸也, 大坪和香子, 宮内啓介, 遠藤銀朗

    東北学院大学環境防災工学研究所紀要(CD-ROM)　(21)　ROMBUNNO.2-4　2010/03

  64. 0605 GenomeMatcher : GUI tool for genomic analysis

    Ohtsubo Yoshiyuki, Ohtsubo Wakako, Nagata Yuji, Tsuda Masataka

    バイオエンジニアリング講演会講演論文集　2009　(22)　90-90　2010/01/08

    Publisher: 一般社団法人日本機械学会

    DOI： 10.1299/jsmebio.2009.22.90 　

    eISSN： 2424-2829

  65. 高温処理コンポスト化プロセスにおける真核菌類の出現と消長に関する研究

    進藤絵里香, 大坪和香子, 上田裕一, 上田英代, 宮内啓介, 遠藤銀朗

    環境バイオテクノロジー学会大会プログラム講演要旨集　40th　59　2010

  66. P-30 Investigation of denitrifying bacteria involved in N20 reduction during livestock waste composting(Poster Session)

    IKEDA-OHTSUBO WAKAKO, TAKAHASHI RYO, TAKAHASHI MASAYUKI, SHINDO ERIKA, UEDA HIDEYO, UEDA YASUICHI, MIYAUCHI KEISUKE, ENDO GINRO

    日本微生物生態学会講演要旨集　(26)　109-109　2010

    Publisher: 日本微生物生態学会

  67. 1A-5 畜産廃棄物のコンポスト化過程におけるN2O発生に関与する微生物の解析(口頭発表)

    大坪 和香子, 荒木 伸也, 山田 剛史, 上田 英代, 上田 裕一, 宮内 啓介, 遠藤 銀朗

    日本微生物生態学会講演要旨集　(25)　3-3　2009

    Publisher: 日本微生物生態学会

Show all ︎Show first 5

Books and Other Publications 1

  1. Japan after 3/11: Global Perspectives on the Earthquake, Tsunami, and Fukushima Meltdown

    Yukiko Dejima, Wakako Ikeda-Ohtsubo

    University Press of Kentucky　2016

Presentations 22

  1. Lactobacillus plantarum CRL1506における抗ウイルス性イムノジェニクスの解明

    舟橋 龍太郎, 水野 滉也, 友常 加恵, 友清 帝, Md. Aminul Islam、Leonardo Albarracín, 大坪 和香子, 麻生 久, Julio Villena, 北澤 春樹

    日本畜産学会第128回大会　2021/03/29

  2. ウシ乳腺上皮細胞によるイムノバイオティクスの選抜・評価系構築

    福山 皓太朗, 高木 理宏, Md. Aminul Islam, Leonardo Albarracin, 須田 義人, 大坪 和香子, 野地 智法, 麻生 久, Julio Villena, 北澤 春樹

    日本畜産学会第128回大会　2021/03/29

  3. Immunododulatory properties and the genomic studies of immunobiotics and immunosynbiotic characterization

    2021/03/29

  4. Interhost dispersal of intestinal bacteria between horses in the same pasture and stables

    Wakako Ikeda-Ohtsubo, Jasper, A.J. Eikelboom, Yudai Yamakawa, Hiroshi Yoneyama, Junko Nishimura, Haruki Kitazawa

    The 33rd Annual Meeting of Japanese Society of Equine Science　2020/11/30

  5. 新規イムノバイオティクス候補となる酪農乳酸菌の 特異的検出・同定のための新規プライマーの開発

    土田咲絵, 大坪和香子, 須田義人, 北澤春樹

    日本乳酸菌学会2020年度大会　2020/11/27

  6. オキナワモズクフコイダンのプレバイオティクス効果の研究

    有川優希, 大坪和香子

    第 53 回日本無菌生物ノートバイオロジー学会総会　2020/01/25

  7. Microarray analysis of bovine mammary epithelial cells stimulated with mastitis pathogens

    Michihiro Takagi, Ryoya Komatsu, Julio Villena, Leonardo Albarracin, Md Aminul Islam, Yoshihito Suda, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Hisashi Aso, Haruki Kitazawa

    日本畜産学会第125回大会　2019/03/29

  8. Construction of porcine immunobiotics library

    Yuhka Nakano, Yuki Masumizu, Ryusuke Ohgi, Leonardo Albarracin, Wakako Ohtsubo, Md Aminul Islam, Yoshihito Suda, Julio Villena, Tomonori Nochi, Haruki Kitazawa

    2019/03/29

  9. Selection of wakame utilizing lactobacilli adhering to porcine intestinal mucin and epitheliocytes

    Binghui Zhou, Yuki Masumizu, Yuhka Nakano, Yuhki Indo, Ryoya Komatsu, Md Aminul Islam, Wakako Ohtsubo, Yoshihito Suda, Keiichi Suzuki, Hisashi Aso, Haruki Kitazawa

    日本畜産学会第125回大会　2019/03/29

  10. Ryoya Komatsu, Hikaru Iida, Leonardo Albarracín, Md Aminul Islam, Wakako Ikeda-Ohtsubo, Hisashi Aso, Noriyuki Iwabuchi, Jin-zhong Xiao, Tadao Saito, Julio Villena, Haruki Kitazawa

    Ryoya Komatsu, Hikaru Iida, Leonardo Albarracín, Md Aminul Islam, Wakako Ikeda-Ohtsubo, Hisashi Aso, Noriyuki Iwabuchi, Jin-zhong Xiao, Tadao Saito, Julio Villena, Haruki Kitazawa

    2019/03/28

  11. Adhesion ability of bifidobacteria to human intestine

    Binghui Zhou, Noriyuki Iwabuchi, Ohtsubo (Ikeda) Wakako, Jin-zhong Xiao, Tadao Saito, Haruki Kitazawa

    2018/09/14

  12. 海藻多糖のプレバイオティクス機能の探索

    有川優希, 大坪和香子, 木村一雅, 伊波匡彦, 嘉手納 築, 齋藤忠夫, 北澤春樹

    酪農科学シンポジウム2018　2018/09/14

  13. TLR2 and 4 ligands stimulate inflammation/adipogenesis in porcine adipocytes

    Igata Manami, AKM Humayun Kober, Tada Asuka, Albarracin Leonardo, Ikeda-Ohtsubo Wakako, Aso Hisashi, Yoda Kazutoyo, Miyazawa Kenji, He Fang, Saito Tadao, Villena Julio, Kitazawa Haruki

    2018/03/28

  14. Development of immunobiotic evaluation system on PIE cells stimulated by DSS

    Sato Nana, Kober Humayun, Albarracin Leonardo, Indoh Yuhki, Ohtsubo Wakako, Aso Hisashi, Saito Tadao, Villena Julio, Haruki Kitazawa

    2018/03/28

  15. Research on adhesion property of Bifidobacteria strains to human intestine

    Zhou Binghui, Ikeda-Ohtsubo Wakako, Kitazawa Haruki, Saito Tadao

    The 124th Annual Meeting of Japanese Society of Animal Science　2018/03/28

  16. ハチミツに含まれるオリゴ糖のビフィズス菌による資化性

    大坪 和香子, 澤田 侑希, 北澤 春樹, 齋藤 忠夫

    日本農芸化学会2018年度大会　2018/03/16

  17. ハチミツに含まれるオリゴ糖の特徴とプレバイオティクス効果

    澤田侑希, 大坪和香子

    2018/01/26

  18. ヒト腸管粘膜中に常在する硫酸還元細菌の検出と同定

    日下練介, 大坪和香子

    第５１回日本無菌生物ノートバイオロジー学会　2018/01/26

  19. Exploration Of Sulfate-Reducing Bacteria Colonizing The Intestinal Tracts International-presentation

    W. Ikeda-Ohtsubo, R. Kusaka

    Microbiology Centennial Symposium, Wageningen University　2017/10/19

  20. Identification of gut bacteria of leaf beetles feeding on plants with high oxalate contents International-presentation

    W. Ikeda-Ohtsubo, A. Miyagi, M. K. F. Lee, H. Mori, N. Ojima, A. Mikaelyan, K. Kurokawa, T. Saito

    International Congress of Bacteriology and Applied Microbiology (IUMS 2017)　2017/07/18

  21. Identification of sulfate-reducing bacteria in human intestinal mucus International-presentation

    W. Ikeda-Ohtsubo, Rensuke Akiba, Haruki Kitazawa, Tadao Saito

    Joint Congress of the 19th International Symposium on Gnotobiology (XIX ISG), the 50th Congress of Japanese Association of Germfree Life and Gnotobiology (L JAGG) and the 39th Congress of the Society for Microbial Ecology and Disease (XXXIX SOMED)　2017/06/08

  22. in Japanese

    The 124th Annual Meeting of Japanese Society of Animal Science　2017/03/28

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Research Projects 17

  1. 動物性食品の健康的摂取の指針となる小腸フローラ制御方法の開発

    大坪 和香子

    Offer Organization: 科学技術振興機構

    System: 戦略的な研究開発の推進 創発的研究支援事業

    Institution: 東北大学

    2023 - 2029

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    乳・肉・卵などの動物性食品には必須アミノ酸「トリプトファン」が多く含まれますが、このアミノ酸は小腸で吸収されるほか、小腸内に生息する細菌群(小腸フローラ)によって糖尿病や認知症等の予防・改善効果が知られる「インドール誘導体」に変換されています。しかし、この「インドール誘導体」を作る腸内細菌の実態はよく知られていません。本研究では、小腸内において有益な「インドール誘導体」産生を促進する新手法の提案を目指し、「インドール誘導体」産生小腸細菌の性状解明と制御方法の開発に取り組みます。

  2. 家畜健全育成向上戦略：イムノバイオティクスと自然免疫受容体の腸-肺軸シンフォニー

    北澤 春樹, 西山 啓太, 大坪 和香子, Binghui Zhou, 野地 智法, 竹之内 敬人, 新開 浩樹, 須田 義人

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(A)

    Institution: 東北大学

    2023/04/01 - 2027/03/31

  3. 食品成分によるビフィズス菌細胞外多糖の産生誘導メカニズムの解明と応用基盤の構築

    大坪 和香子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2023/04/01 - 2026/03/31

  4. Development of a new renal therapy through Gut-renal axis

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2021/04/01 - 2024/03/31

  5. 内在性抗菌因子PGRPに着目した育種と飼養の抗病性シナジー強化による健全畜産戦略

    北澤 春樹, 大坪 和香子, 麻生 久, 野地 智法, 上西 博英, 宮崎 綾子, 宗田 吉広, 須田 義人

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(A)

    Category: 基盤研究(A)

    Institution: 東北大学

    2019/04/01 - 2023/03/31

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    本研究は、家畜が有する抗菌因子に着目し、遺伝育種学的手法とイムノバイオティクスの発展的融合により、家畜の抗病性機能強化から抗菌剤に依存しない新たな健全育成技術基盤の創出を目的とする。本年度は、以下の成果が得られた。 【課題１】豚におけるPGLYRPの遺伝子多型と疾病関連形質との関係性解明： 豚PGLYRP1～4について、デュロック種12頭を用いてコード領域その上流5kbの多様性解析を行った。上流配列にはPGLYRP1～4遺伝子でそれぞれ1・10・31・31個のSNPが検出された。また、コード領域にPGLYRP2～4遺伝子でそれぞれ3・7・2個の非同義置換SNPが検出された。 【課題２】PGLYRP増強活性を有するイムノバイオティクスの選抜と抗病性機構解明：豚由来乳酸菌ライブラリーより、PIE細胞を用いたパターン認識受容体を介する抗病性免疫調節機能の観点から４菌株を選抜した。また、イムノバイオティクス候補菌株のPGLYRP発現増強に関する評価を行うと共に、豚由来樹状細胞におけるPGLYRPの発現増強について評価した。 【課題３】HPCD子豚を用いたイムノバイオティクス候補菌株の評価：課題２で選抜された４菌株についてノトバイオート子豚への経口投与を行い、個体レベルでの影響、免疫応答について検討した。また、菌株投与によるロタウイルス感染増殖抑制や症状軽減効果について検討を開始した。 【課題４】一般豚およびPGLYRP選抜豚における健全育成・産肉性向上の実証： LWD豚を供試しイムノバイオティクス候補菌株投与後、血清における生化学検査とサイトカイン濃度、糞便におけるロタウイルス検出、毒素原性大腸菌の検出、腸管組織における免疫関連遺伝子の測定を行うことで、健康性への効果を評価することが可能と考えられた。

  6. Understanding growth-promoting effect of intestinal butyrate-producing bacteria by fucoinda extracted from Okinawa Mozuku

    Wakako Ikeda-Ohtsubo

    Offer Organization: LOTTE FOUNDATION

    2020/04 - 2023/03

  7. Development of an evaluation system for anti-disease immunobiotics of livestock and its applied strategy

    Kitazawa Haruki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2016/04/01 - 2020/03/31

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    An in vitro unique anti-disease selection and evaluation system for the livestock-responsive immunobiotics (mucosal immunomodulatory functional probiotics) was developmentally established, and the basic technology for the livestock healthy growth strategy was established via the development and use of this system. Specifically, an infection system for bacterial, viral, and other enteric pathogens was established using intestinal epithelial cell lines derived from livestock, and an evaluation system was constructed using the ability to protect against infection as an parameter. In addition, the evaluation system selected anti-disease immunobiotics and validated the usefulness of this system by in vivo confirmation study.

  8. Characterization of commensal gut bacteria in human and animals

    Offer Organization: Tohoku University

    2018/09 - 2019/06

  9. Development of probiotics for the control of inflammatory bowel disease Competitive

    Ohtsubo Wakako, Kitazawa Haruki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2017/06/30 - 2019/03/31

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    Culture enrichments of human gut microbiota using various polysaccharides as a substrate were performed and bacterial strains affiliated with Lachnospiraceae, which produce butyrate and simultaneously degrade algae polysaccharides, were found. We screened probiotic bifidobacterial strains with an ability to utilize fucose to establish a co-culture of butyrate-producing Lachnospiraceae and probiotics. We also identified bifidobacterial strains with an ability of competitive adhesion to purified human mucosal polysaccharides against sulfate-reducing bacteria, which occurrence has been known to be correlated with inflammatory bowel disease. Our findings offer a new aspect of probiotics for preventing inflammatory bowel disease by promoting butyrate-producing bacteria or by preventing sulfate-reducing bacteria in human intestine.

  10. 日本人腸内フローラによるオキナワモズクフコイダン分解メカニズムの解明 Competitive

    大坪 和香子

    Offer Organization: 一般財団法人 東和食品研究振興会

    System: 2018年度 学術奨励金

    2018/06 - 2019/03

  11. 外来雑草エゾノギシギシを摂食するコガタルリハムシの腸内シュウ酸分解細菌の共生機能の解明 Competitive

    大坪 和香子

    Offer Organization: 公益財団法人発酵研究所

    System: 平成２９年度一般研究助成

    2017/04 - 2019/03

  12. 海藻由来食物繊維を利用した新規シンバイオティックヨーグルトの開発研究 Competitive

    大坪 和香子

    Offer Organization: 公益財団法人三島海雲記念財団

    System: 平成２９年度学術研究奨励金

    2017/07 - 2018/06

  13. Ecophysiology of gut bacteria of leaf beetles Gastrophysa atrocyanea

    Wakako Ohtsubo, MIYAGI ATSUKO

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    2013/04/01 - 2016/03/31

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    We conducted 16S rRNA gene analysis and metagenomic analysis of green dock leaf beetles Gastrophysa atrocyanea and Gastrophysa viridula. Different uncultured groups of Enterobacteriaceae, including genera Rahnella, Pantoea, and Serratia dominated the gut microbiota of each beetle, which reflects the location of correction sites. The metagenomic library contained a gene homolog of oxalate decarboxylase, which may be involved in degradation of oxalate, a major recalcitrant compound in Rumex Obtusifolius that the beetles fed on. The homolog showed the highest identity to those from genomes of Enterobacteriaceae species, which suggests its origin from the gut members dominating the Gastrophysa beetles.

  14. コガタルリハムシ腸内共生乳酸菌の機能生態の解明 研究課題 Competitive

    大坪 和香子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的萌芽研究

    2013/06 - 2016/03

  15. 畜産廃棄物堆肥中の亜酸化窒素還元除去能を有する脱窒細菌の生態解明 Competitive

    大坪 和香子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 若手研究(B)

    2011/04 - 2013/03

  16. Microbial ecology of nitrous oxide-reducing denitrifying bacteria in the livestock waste composts

    OHTSUBO Wakako

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku Gakuin University

    2011 - 2012

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    In this study we performed composting experiments using solidwastes from piggery wastewater treatment processes. Our results indicated that the timing of N_2O emission follows emergence of ammonia-oxidizing bacteria, which produce denitrification substrates such as nitrate and nitrite. We also found that hyperthermophilic pre-treatment reduces N_2O emission from composting, which is suggest to have been caused by denitrifying bacteria with N_2O-reducing activities. Phylogenetic analyses showed that community structures of ammonia-oxidizing bacteria and denitrifying bacteria dynamically changed as composting proceeded. Denitrifying bacterial strains isolated from our compost samples were able to perform denitrification under oxic or microoxic conditions. These findings collectively suggest that N_2O emission patterns during composting is strongly affected by community structures and functions of ammonia-oxidizing bacteria and denitrifying bacteria, which should be effectively controlled to reduce N_2O emission from composting processes.

  17. Study of maturation of gut microbiota using insect models

    Offer Organization: Tohoku University

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Social Activities 2

  1. 【東北学院大学 工学総合研究所公開講座】『微生物』が拓く安全・安心な社会

    2014/10/15 -

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    講演「ミクロな動物園 ～腸内微生物の豊かな個性～」

  2. 桜美林大学 2012 年国際シンポジウム — The 2011 Japanese Tsunami: Disaster, Response, and Recovery

    2012/10/19 -

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    講演「インターネット時代における親と子：2011年三陸沖地震津波後」