Details of the Researcher

PHOTO

Kan Saito
Section
Graduate School of Dentistry
Job title
Professor
Degree

  • 博士（歯学） (Nagasaki University)

Research History 10

  • 2024/04 - Present
    Tohoku University Graduate School of Dentistry　Division of Pediatric Dentistry, Department of Community Social Dentistry　professor

  • 2023/04 - Present
    Miyagi Gakuin Women's University

  • 2018/04 - Present
    菅原学園　仙台総合医療大学校　非常勤講師

  • 2015/04 - 2024/03
    新英学園　仙台歯科技工士専門学校　非常勤講師

  • 2015/04 - 2024/03
    Tohoku University　Pediatric dentistry

  • 2016/04 - 2019/03
    東北大学歯学部附属　歯科技工士学校　非常勤講師

  • 2013/02 - 2015/03
    Tohoku University　Pediatric dentistry

  • 2009 - 2011
    ヘルシンキ大学バイオテクノロジー研究所 客員研究員

  • 2004 - 2009
    Nagasaki University　Pediatric dentistry　research associate

  • 2011 -
    Nagasaki University　Pediatric dentistry

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Education 4

  • Nagasaki University

    - 2004

  • Nagasaki University　Graduate School, Division of Dental Research

    - 2004

  • Nagasaki University　School of Dentistry

    - 2000

  • Nagasaki University　Faculty of Dentistry

    - 2000

Committee Memberships 6

  • 日本小児口腔外科学会　代議員

    2024/11 - Present

  • 日本小児歯科学会　理事

    2024/05 - Present

  • 宮城県小児保健協会　理事

    2024/04 - Present

  • 日本小児歯科学会　ガイドライン作成委員

    2018/09 - Present

  • 日本小児歯科学会　代議員

    2018/04 - 2024/05

  • 日本小児歯科学会　小児歯科学雑誌編集委員

    2013/04 - 2014/03

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Professional Memberships 4

  • 障害者歯科学会

  • 歯科基礎医学会

  • 日本細菌学会

  • 日本小児歯科学会

Research Interests 3

  • 小児歯科

  • 免疫学

  • 硬組織

Research Areas 1

  • Life sciences / Developmental dentistry /

Awards 10

  1. 第62回日本小児歯科学会大会 SHOFU AWARD

    2024/05　日本小児歯科学会

  2. 第62回日本小児歯科学会大会 優秀発表賞

    2024/05　日本小児歯科学会

  3. 第41回日本小児歯科北日本地方会 優秀発表賞

    2023/10　日本小児歯科学会

  4. 61st Annual Congress of the Japanese Society of Pediatric Dentistry, Best Presentation Award

    2023/05　Japanese Society of Pediatric Dentistry

  5. 第56回 日本小児歯科学会 Lion award

    2018/05　日本小児歯科学会

  6. 第51回 日本小児歯科学会 優秀発表賞

    2013/05　日本小児歯科学会

  7. 第11回 口腔医科学フロンティア 奨励賞

    2013/03　口腔医科学フロンティア

  8. 第47回 日本小児歯科学会 奨励賞

    2009/05　日本小児歯科学会

  9. 第42回 日本小児歯科学会 デンツプライ賞

    2004/06　日本小児歯科学会

  10. 平成15年度 長崎大学学長賞

    2004/03　長崎大学

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Papers 59

  1. Enamel protection in pediatric patients using a bracket bonding agent with a bioactive monomer

    Manami Tadano, Ryoko Hino, Seira Hoshikawa, Yuma Tsuruta, Shinji Otake, Yuta Chiba, Aya Yamada, Satoshi Fukumoto, Yuriko Maruya, Kan Saito

    Pediatric Dental Journal　2025/12

    DOI： 10.1016/j.pdj.2025.100368 　

  2. Expression patterns of desmosome family members during tooth development and the role of Desmocollin-3 in cytodifferentiation of stratum intermedium

    Triana Marchelina, Yuta Chiba, Shinji Otake, Li Wanshu, Hiroshi Sato, Yumiko Nakashima, Asuna Sugimoto, Tsutomu Iwamoto, Aya Yamada, Kan Saito, Satoshi Fukumoto

    Archives of Oral Biology　2025/12

    DOI： 10.1016/j.archoralbio.2025.106404 　

  3. Integration of single cell analysis of dental mesenchyme and human disease database identifies the responsible gene of dentin disorders

    Hidenori Kokubo, Yuta Chiba, Triana Marchelina, Kifu Miyata, Hidenori Oikawa, Rion Sho, Kan Saito, Aya Yamada, Satoshi Fukumoto

    Pediatric Dental Journal　2025/04

    DOI： 10.1016/j.pdj.2024.100337 　

  4. Case Report: Dental treatment under general anesthesia and dental management of a child with congenital ichthyosis Peer-reviewed

    Ryoko Hino, Yuta Chiba, Yuriko Maruya, Manami Tadano, Shinji Otake, Seira Hoshikawa, Yoji Sasahara, Kan Saito

    Frontiers in Dental Medicine　5　2024/10/17

    Publisher: Frontiers Media SA

    DOI： 10.3389/fdmed.2024.1481658 　

    eISSN： 2673-4915

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    Congenital ichthyosis is a disease in which the stratum corneum on the surface of the skin becomes thick from the time of the fetus and the barrier function of the skin is impaired. Congenital ichthyosis is a genetic disorder that causes ectodermal abnormalities and sometimes affects skin, nails, and tooth enamel. Therefore, some patients require special care in their daily life and during dental treatments. Here, the authors report a case of congenital ichthyosis that developed into severe dental caries at two years and nine months of age. The authors performed whole-exome sequencing in his peripheral blood and found that the patient had compound heterozygous mutations in ALOX12B gene (c.159C>G and c.1579G>A), which is responsible for autosomal recessive congenital ichthyosis-2 (MIM#2421000). Mutation of c.159C>G is a nonsense mutation that has never been reported, therefore novel symptoms might have found. The patients exhibited severe caries by hypoplastic teeth. Here, the authors report the treatment of dental caries in a patient with congenital ichthyosis under general anesthesia and its oral management until mixed dentition.

  5. Orthodontic management of severe inversely impacted maxillary central incisors: a case series Peer-reviewed

    Yuriko Maruya, Ryoko Hino, Manami Tadano, Seira Hoshikawa, Shinji Otake, Yuta Chiba, Kan Saito

    Frontiers in Oral Health　5　2024/10/16

    Publisher: Frontiers Media SA

    DOI： 10.3389/froh.2024.1474190 　

    eISSN： 2673-4842

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    Background Abnormal positioning and dislocation of the central incisor can disturb tooth eruption. Generally, inversely impacted maxillary central incisors do not erupt naturally. Performing traction and applied extrusion of an inversely impacted maxillary central incisor with a high inclination angle of the crown is challenging. This study aimed to examine the possibility of orthodontic treatment for severely inversely impacted maxillary central incisors in a series of case studies. Methods The inclination angle of the tooth crown, curvature of the tooth root, and length of the formed tooth root were measured using radiography. The teeth were then fenestrated and traction was applied using a lingual arch appliance with elastics. Results The average crown axis inclination was 113°, the degree of root curvature was 97.3°, and the root formation was 36.1%. Although the crown axis inclination and root curvature were severe, all the incisors were aligned in the correct position as vital teeth through surgical and orthodontic treatments. Conclusions Traction should be performed in the early period of incisor development when root formation is not progressing, regardless of the tooth angle.

  6. S100a6 knockdown promotes the differentiation of dental epithelial cells toward the epidermal lineage instead of the odontogenic lineage Peer-reviewed

    Shinji Otake, Kan Saito, Yuta Chiba, Aya Yamada, Satoshi Fukumoto

    The FASEB Journal　38　(7)　2024/04/15

    DOI： 10.1096/fj.202302412RR 　

  7. The correlation between the inner canthal distance and maxillary mesiodens in children Peer-reviewed

    Manami Tadano, Yasunori Matsunaga, Kan Saito, Yuria Suzuki, Tomoaki Nakamura, Seira Hoshikawa, Mitsuki Chiba, Ryoko Hino, Yuriko Maruya, Emiko Fukumoto, Aya Yamada, Satoshi Fukumoto

    Pediatric Dental Journal　2023/08

    DOI： 10.1016/j.pdj.2023.04.001 　

  8. Deficiency of G protein-coupled receptor Gpr111/Adgrf2 causes enamel hypomineralization in mice by alteration of the expression of kallikrein-related peptidase 4 (Klk4) during pH cycling process. International-journal Peer-reviewed

    Yuta Chiba, Keigo Yoshizaki, Hiroshi Sato, Tomoko Ikeuchi, Craig Rhodes, Mitsuki Chiba, Kan Saito, Takashi Nakamura, Tsutomu Iwamoto, Aya Yamada, Yoshihiko Yamada, Satoshi Fukumoto

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology　37　(4)　e22861　2023/04

    DOI： 10.1096/fj.202202053R 　

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    Enamel is formed by the repetitive secretion of a tooth-specific extracellular matrix and its decomposition. Calcification of the enamel matrix via hydroxyapatite (HAP) maturation requires pH cycling to be tightly regulated through the neutralization of protons released during HAP synthesis. We found that Gpr115, which responds to changes in extracellular pH, plays an important role in enamel formation. Gpr115-deficient mice show partial enamel hypomineralization, suggesting that other pH-responsive molecules may be involved. In this study, we focused on the role of Gpr111/Adgrf2, a duplicate gene of Gpr115, in tooth development. Gpr111 was highly expressed in mature ameloblasts. Gpr111-KO mice showed enamel hypomineralization. Dysplasia of enamel rods and high carbon content seen in Gpr111-deficient mice suggested the presence of residual enamel matrices in enamel. Depletion of Gpr111 in dental epithelial cells induced the expression of ameloblast-specific protease, kallikrein-related peptidase 4 (Klk4), suggesting that Gpr111 may act as a suppressor of Klk4 expression. Moreover, reduction of extracellular pH to 6.8 suppressed the expression of Gpr111, while the converse increased Klk4 expression. Such induction of Klk4 was synergistically enhanced by Gpr111 knockdown, suggesting that proper enamel mineralization may be linked to the modulation of Klk4 expression by Gpr111. Furthermore, our in vitro suppression of Gpr111 and Gpr115 expression indicated that their suppressive effect on calcification was additive. These results suggest that both Gpr111 and Gpr115 respond to extracellular pH, contribute to the expression of proteolytic enzymes, and regulate the pH cycle, thereby playing important roles in enamel formation.

  9. Single-cell RNA-sequence of dental epithelium reveals responsible genes of dental anomalies in human Peer-reviewed

    Kifu Miyata, Yuta Chiba, Triana Marchelina, Saori Inada, Sae Oka, Kan Saito, Aya Yamada, Satoshi Fukumoto

    Pediatric Dental Journal　2023/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.pdj.2023.03.004 　

    ISSN： 0917-2394

  10. GSK3beta inhibitor-induced dental mesenchymal stem cells regulate ameloblast differentiation International-journal Peer-reviewed

    Aya Yamada, Keigo Yoshizaki, Kan Saito, Masaki Ishikawa, Yuta Chiba, Seira Hoshikawa, Mitsuki Chiba, Ryoko Hino, Yuriko Maruya, Hiroshi Sato, Keiji Masuda, Haruyoshi Yamaza, Takashi Nakamura, Tsutomu Iwamoto, Satoshi Fukumoto

    Journal of Oral Biosciences　64　(4)　400-409　2022/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.job.2022.10.002 　

    ISSN： 1349-0079

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    OBJECTIVES: Epithelial-mesenchymal interactions are extremely important in tooth development and essential for ameloblast differentiation, especially during tooth formation. We aimed to identify the type of mesenchymal cells important in ameloblast differentiation. METHODS: We used two types of cell culture systems with chambers and found that a subset of dental mesenchymal cells is important for the differentiation of dental epithelial cells into ameloblasts. Among odontogenic mesenchymal cells, dental pulp stem cell-like cells induced the expression of ameloblast differentiation markers. Therefore, we induced dental pulp stem cell-like cells from dental pulp stem cells using the small molecule compound BIO (a GSK-3 inhibitor IX) to clarify the mechanism involved in inducing ameloblast differentiation of dental pulp stem cells. RESULTS: The BIO-induced dental pulp cells promoted the expression of mesenchymal stem cell markers Oct3/4 and Bcrp1. Furthermore, we used artificial dental pulp stem cells induced by BIO to identify the molecules expressed in dental pulp stem cells required for ameloblast differentiation. Panx3 expression was induced in the dental pulp stem cell through interaction with the dental epithelial cells. In addition, ATP release from cells increased in Panx3-expressing cells. We also confirmed that ATP stimulation is accepted in dental epithelial cells. CONCLUSIONS: These results showed that the Panx3 expressed in dental pulp stem cells is important for ameloblast differentiation and that ATP release by Panx3 may play a role in epithelial-mesenchymal interaction.

  11. The Retention Effect of Resin-Based Desensitizing Agents on Hypersensitivity—A Randomized Controlled Trial Peer-reviewed

    Manami Tadano, Tomoaki Nakamura, Seira Hoshikawa, Ryoko Hino, Yuriko Maruya, Aya Yamada, Satoshi Fukumoto, Kan Saito

    Materials　15　(15)　5172　2022/07/26

    DOI： 10.3390/ma15155172 　

  12. von Willebrand factor D and EGF domains regulate ameloblast differentiation and enamel formation International-journal Peer-reviewed

    Kokoro Iwata, Keita Kawarabayashi, Keigo Yoshizaki, Tian Tian, Kan Saito, Asuna Sugimoto, Rika Kurogoushi, Aya Yamada, Akihito Yamamoto, Yasuei Kudo, Naozumi Ishimaru, Satoshi Fukumoto, Tsutomu Iwamoto

    Journal of Cellular Physiology　237　(3)　1964-1979　2021/12/26

    Publisher: Wiley

    DOI： 10.1002/jcp.30667 　

    ISSN： 0021-9541

    eISSN： 1097-4652

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    Cell- and tissue-specific extracellular matrix (ECM) composition plays an important role in organ development, including teeth, by regulating cell behaviors, such as cell proliferation and differentiation. Here, we demonstrate for the first time that von Willebrand factor D and epidermal growth factor (EGF) domains (Vwde), a previously uncharacterized ECM protein, is specifically expressed in teeth and regulates cell proliferation and differentiation in inner enamel epithelial cells (IEEs) and enamel formation. We identified the Vwde as a novel ECM protein through bioinformatics using the NCBI expressed sequence tag database for mice. Vwde complementary DNA encodes 1773 amino acids containing a signal peptide, a von Willebrand factor type D domain, and tandem calcium-binding EGF-like domains. Real-time polymerase chain reaction demonstrated that Vwde is highly expressed in tooth tissue but not in other tissues including the brain, lung, heart, liver, kidney, and bone. In situ hybridization revealed that the IEEs expressed Vwde messenger RNA in developing teeth. Immunostaining showed that VWDE was localized at the proximal and the distal ends of the pericellular regions of the IEEs. Vwde was induced during the differentiation of mouse dental epithelium-derived M3H1 cells. Vwde-transfected M3H1 cells secreted VWDE protein into the culture medium and inhibited cell proliferation, whereas ameloblastic differentiation was promoted. Furthermore, Vwde increased the phosphorylation of extracellular signal-regulated kinase 1/2 and protein kinase B and strongly induced the expression of the intercellular junction protein, N-cadherin (Ncad). Interestingly, the suppression of endogenous Vwde inhibited the expression of Ncad. Finally, we created Vwde-knockout mice using the CRISPR-Cas9 system. Vwde-null mice showed low mineral density, rough surface, and cracks in the enamel, indicating the enamel hypoplasia phenotype. Our findings suggest that Vwde assembling the matrix underneath the IEEs is essential for Ncad expression and enamel formation.

  13. Evaluation of a Hypersensitivity Inhibitor Containing a Novel Monomer That Induces Remineralization—A Case Series in Pediatric Patients Peer-reviewed

    Manami Tadano, Aya Yamada, Yuriko Maruya, Ryoko Hino, Tomoaki Nakamura, Seira Hoshikawa, Satoshi Fukumoto, Kan Saito

    Children　2021/12/16

    DOI： 10.3390/children8121189 　

  14. The tooth‐specific basic helix‐loop‐helix factor AmeloD promotes differentiation of ameloblasts International-journal Peer-reviewed

    LingLing Jia, Yuta Chiba, Kan Saito, Keigo Yoshizaki, Tian Tian, Xu Han, Kanji Mizuta, Mitsuki Chiba, Xin Wang, Shahad Al Thamin, Aya Yamada, Satoshi Fukumoto

    Journal of Cellular Physiology　237　(2)　1597-1606　2021/11/23

    Publisher: Wiley

    DOI： 10.1002/jcp.30639 　

    ISSN： 0021-9541

    eISSN： 1097-4652

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    Tissue-specific basic helix-loop-helix (bHLH) transcription factors play an important role in cellular differentiation. We recently identified AmeloD as a tooth-specific bHLH transcription factor. However, the role of AmeloD in cellular differentiation has not been investigated. The aim of this study was to elucidate the role of AmeloD in dental epithelial cell differentiation. We found that AmeloD-knockout (AmeloD-KO) mice developed an abnormal structure and altered ion composition of enamel in molars, suggesting that AmeloD-KO mice developed enamel hypoplasia. In molars of AmeloD-KO mice, the transcription factor Sox21 encoding SRY-Box transcription factor 21 and ameloblast differentiation marker genes were significantly downregulated. Furthermore, overexpression of AmeloD in the dental epithelial cell line M3H1 upregulated Sox21 and ameloblast differentiation marker genes, indicating that AmeloD is critical for ameloblast differentiation. Our study demonstrated that AmeloD is an important transcription factor in amelogenesis for promoting ameloblast differentiation. This study provides new insights into the mechanisms of amelogenesis.

  15. Integration of Single-Cell RNA- and CAGE-seq Reveals Tooth-Enriched Genes International-journal Peer-reviewed

    Y. Chiba, K. Yoshizaki, T. Tian, K. Miyazaki, D. Martin, K. Saito, A. Yamada, S. Fukumoto

    Journal of Dental Research　002203452110497-002203452110497　2021/11/20

    Publisher: SAGE Publications

    DOI： 10.1177/00220345211049785 　

    ISSN： 0022-0345

    eISSN： 1544-0591

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    Organ development is dictated by the regulation of genes preferentially expressed in tissues or cell types. Gene expression profiling and identification of specific genes in organs can provide insights into organogenesis. Therefore, genome-wide analysis is a powerful tool for clarifying the mechanisms of development during organogenesis as well as tooth development. Single-cell RNA sequencing (scRNA-seq) is a suitable tool for unraveling the gene expression profile of dental cells. Using scRNA-seq, we can obtain a large pool of information on gene expression; however, identification of functional genes, which are key molecules for tooth development, via this approach remains challenging. In the present study, we performed cap analysis of gene expression sequence (CAGE-seq) using mouse tooth germ to identify the genes preferentially expressed in teeth. The CAGE-seq counts short reads at the 5′-end of transcripts; therefore, this method can quantify the amount of transcripts without bias related to the transcript length. We hypothesized that this CAGE data set would be of great help for further understanding a gene expression profile through scRNA-seq. We aimed to identify the important genes involved in tooth development via bioinformatics analyses, using a combination of scRNA-seq and CAGE-seq. We obtained the scRNA-seq data set of 12,212 cells from postnatal day 1 mouse molars and the CAGE-seq data set from postnatal day 1 molars. scRNA-seq analysis revealed the spatiotemporal expression of cell type–specific genes, and CAGE-seq helped determine whether these genes are preferentially expressed in tooth or ubiquitously. Furthermore, we identified candidate genes as novel tooth-enriched and dental cell type–specific markers. Our results show that the integration of scRNA-seq and CAGE-seq highlights the genes important for tooth development among numerous gene expression profiles. These findings should contribute to resolving the mechanism of tooth development and establishing the basis for tooth regeneration in the future.

  16. Connexin 43-Mediated Gap Junction Communication Regulates Ameloblast Differentiation via ERK1/2 Phosphorylation. International-journal Peer-reviewed

    Aya Yamada, Keigo Yoshizaki, Masaki Ishikawa, Kan Saito, Yuta Chiba, Emiko Fukumoto, Ryoko Hino, Seira Hoshikawa, Mitsuki Chiba, Takashi Nakamura, Tsutomu Iwamoto, Satoshi Fukumoto

    Frontiers in physiology　12　748574-748574　2021

    DOI： 10.3389/fphys.2021.748574 　

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    Connexin 43 (Cx43) is an integral membrane protein that forms gap junction channels. These channels mediate intercellular transport and intracellular signaling to regulate organogenesis. The human disease oculodentodigital dysplasia (ODDD) is caused by mutations in Cx43 and is characterized by skeletal, ocular, and dental abnormalities including amelogenesis imperfecta. To clarify the role of Cx43 in amelogenesis, we examined the expression and function of Cx43 in tooth development. Single-cell RNA-seq analysis and immunostaining showed that Cx43 is highly expressed in pre-secretory ameloblasts, differentiated ameloblasts, and odontoblasts. Further, we investigated the pathogenic mechanisms of ODDD by analyzing Cx43-null mice. These mice developed abnormal teeth with multiple dental epithelium layers. The expression of enamel matrix proteins such as ameloblastin (Ambn), which is critical for enamel formation, was significantly reduced in Cx43-null mice. TGF-β1 induces Ambn transcription in dental epithelial cells. The induction of Ambn expression by TGF-β1 depends on the density of the cultured cells. Cell culture at low densities reduces cell-cell contact and reduces the effect of TGF-β1 on Ambn induction. When cell density was high, Ambn expression by TGF-β1 was enhanced. This induction was inhibited by the gap junction inhibitors, oleamide, and 18α-grycyrrhizic acid and was also inhibited in cells expressing Cx43 mutations (R76S and R202H). TGF-β1-mediated phosphorylation and nuclear translocation of ERK1/2, but not Smad2/3, were suppressed by gap junction inhibitors. Cx43 gap junction activity is required for TGF-β1-mediated Runx2 phosphorylation through ERK1/2, which forms complexes with Smad2/3. In addition to its gap junction activity, Cx43 may also function as a Ca2+ channel that regulates slow Ca2+ influx and ERK1/2 phosphorylation. TGF-β1 transiently increases intracellular calcium levels, and the increase in intracellular calcium over a short period was not related to the expression level of Cx43. However, long-term intracellular calcium elevation was enhanced in cells overexpressing Cx43. Our results suggest that Cx43 regulates intercellular communication through gap junction activity by modulating TGF-β1-mediated ERK signaling and enamel formation.

  17. Transcriptional regulation of the basic helix-loop-helix factor AmeloD during tooth development Peer-reviewed

    Shahad Al Thamin, Yuta Chiba, Keigo Yoshizaki, Tian Tian, Ling Ling Jia, Xin Wang, Kan Saito, Jiyao Li, Aya Yamada, Satoshi Fukumoto

    Journal of Cellular Physiology　236　(11)　7533-7543　2021

    DOI： 10.1002/jcp.30389 　

    ISSN： 0021-9541

    eISSN： 1097-4652

  18. Melnick-Needles syndrome associated molecule, Filamin-A regulates dental epithelial cell migration and root formation Peer-reviewed

    Ryoko Hino, Aya Yamada, Yuta Chiba, Keigo Yoshizaki, Emiko Fukumoto, Tsutomu Iwamoto, Yuriko Maruya, Keishi Otsu, Hidemistu Harada, Kan Saito, Satoshi Fukumoto

    Pediatric Dental Journal　30　(3)　208-214　2020/12

    DOI： 10.1016/j.pdj.2020.09.003 　

    ISSN： 0917-2394

    eISSN： 1880-3997

  19. Identification and function analysis of ameloblast differentiation-related molecules using mouse incisors Peer-reviewed

    Kan Saito, Yuta Chiba, Aya Yamada, Satoshi Fukumoto

    Pediatric Dental Journal　30　(3)　129-138　2020/12

    DOI： 10.1016/j.pdj.2020.08.001 　

    ISSN： 0917-2394

    eISSN： 1880-3997

  20. G protein-coupled receptor Gpr115 (Adgrf4) is required for enamel mineralization mediated by ameloblasts Peer-reviewed

    Yuta Chiba, Keigo Yoshizaki, Kan Saito, Tomoko Ikeuchi, Tsutomu Iwamoto, Craig Rhodes, Takashi Nakamura, Susana de Vega, Robert J. Morell, Erich T. Boger, Daniel Martin, Ryoko Hino, Hiroyuki Inuzuka, Christopher K.E. Bleck, Aya Yamada, Yoshihiko Yamada, Satoshi Fukumoto

    Journal of Biological Chemistry　295　(45)　15328-15341　2020/11/06

    Publisher: American Society for Biochemistry & Molecular Biology (ASBMB)

    DOI： 10.1074/jbc.RA120.014281 　

    ISSN： 0021-9258

    eISSN： 1083-351X

  21. Phosphorylation-dependent osterix degradation negatively regulates osteoblast differentiation International-journal Peer-reviewed

    Seira Hoshikawa, Kouhei Shimizu, Asami Watahiki, Mitsuki Chiba, Kan Saito, Wenyi Wei, Satoshi Fukumoto, Hiroyuki Inuzuka

    FASEB Journal　34　(11)　14930-14945　2020/11/01

    DOI： 10.1096/fj.202001340R 　

    ISSN： 0892-6638

    eISSN： 1530-6860

  22. Expression Patterns of Claudin Family Members During Tooth Development and the Role of Claudin-10 (Cldn10) in Cytodifferentiation of Stratum Intermedium Peer-reviewed

    Xin Wang, Yuta Chiba, Lingling Jia, Keigo Yoshizaki, Kan Saito, Aya Yamada, Man Qin, Satoshi Fukumoto

    Frontiers in Cell and Developmental Biology　8　2020/10/22

    Publisher: Frontiers Media {SA}

    DOI： 10.3389/fcell.2020.595593 　

    ISSN： 2296-634X

    eISSN： 2296-634X

  23. Single-Cell RNA-Sequencing From Mouse Incisor Reveals Dental Epithelial Cell-Type Specific Genes Peer-reviewed

    Yuta Chiba, Kan Saito, Daniel Martin, Erich T. Boger, Craig Rhodes, Keigo Yoshizaki, Takashi Nakamura, Aya Yamada, Robert J. Morell, Yoshihiko Yamada, Satoshi Fukumoto

    Frontiers in Cell and Developmental Biology　8　2020/09/01

    Publisher: Frontiers Media SA

    DOI： 10.3389/fcell.2020.00841 　

    eISSN： 2296-634X

  24. Sox21 Regulates Anapc10 Expression and Determines the Fate of Ectodermal Organ Peer-reviewed

    Kan Saito, Frederic Michon, Aya Yamada, Hiroyuki Inuzuka, Satoko Yamaguchi, Emiko Fukumoto, Keigo Yoshizaki, Takashi Nakamura, Makiko Arakaki, Yuta Chiba, Masaki Ishikawa, Hideyuki Okano, Irma Thesleff, Satoshi Fukumoto

    iScience　23　(7)　101329-101329　2020/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.isci.2020.101329 　

    ISSN： 2589-0042

  25. Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells Peer-reviewed

    Tomoaki Nakamura, Tsutomu Iwamoto, Hannah M. Nakamura, Yuki Shindo, Kan Saito, Aya Yamada, Yoshihiko Yamada, Satoshi Fukumoto, Takashi Nakamura

    Frontiers in Cell and Developmental Biology　8　2020/03/17

    DOI： 10.3389/fcell.2020.00156 　

    ISSN： 2296-634X

    eISSN： 2296-634X

  26. Dentin matrix protein 1 compensates for lack of osteopontin in regulating odontoblastlike cell differentiation after tooth injury in mice Peer-reviewed

    Saito K, Nakatomi M, Ohshima H

    Journal of endodontics　46　(1)　89-96　2020

    DOI： 10.1016/j.joen.2019.10.002 　

  27. Pannexin 3 ER Ca2+ channel gating is regulated by phosphorylation at the Serine 68 residue in osteoblast differentiation Peer-reviewed

    Scientific Reports　2019/12

    DOI： 10.1038/s41598-019-55371-9 　

    ISSN： 2045-2322

  28. 本学小児歯科外来における初診患者の実態調査

    山口 知子, 小山田 優, 日野 綾子, 新垣 真紀子, 齋藤 幹, 山田 亜矢, 福本 敏, 丸谷 由里子

    小児歯科学雑誌　57　(4)　465-472　2019/11

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  29. The transcription factor AmeloD stimulates epithelial cell motility essential for tooth morphology Peer-reviewed

    Yuta Chiba, Bing He, Keigo Yoshizaki, Craig Rhodes, Muneaki Ishijima, Christopher K.E. Bleck, Erin Stempinski, Emily Y. Chu, Takashi Nakamura, Tsutomu Iwamoto, Susana de Vega, Kan Saito, Satoshi Fukumoto, Yoshihiko Yamada

    Journal of Biological Chemistry　294　(10)　3406-3418　2019/03/08

    DOI： 10.1074/jbc.RA118.005298 　

    ISSN： 0021-9258

    eISSN： 1083-351X

  30. The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development. Peer-reviewed

    Han X, Yoshizaki K, Miyazaki K, Arai C, Funada K, Yuta T, Tian T, Chiba Y, Saito K, Iwamoto T, Yamada A, Takahashi I, Fukumoto S

    The Journal of biological chemistry　293　(35)　14572-14584　2018/08

    DOI： 10.1074/jbc.ra118.003373 　

  31. Material properties on enamel and fissure of surface pre-reacted glass-ionomer filler-containing dental sealant Peer-reviewed

    Pediatric Dental Journal　2018/05/24

    DOI： 10.1016/j.pdj.2018.05.001 　

  32. Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains Peer-reviewed

    Arai, C., Yoshizaki, K., Miyazaki, K., Saito, K., Yamada, A., Han, X., Funada, K., Fukumoto, E., Haruyama, N., Iwamoto, T., Takahashi, I., Fukumoto, S.

    Scientific Reports　7　2017

    DOI： 10.1038/srep45181 　

  33. Finger sucking callus as useful indicator for malocclusion in young children Peer-reviewed

    Oyamada, Y., Ikeuchi, T., Arakaki, M., Hino, R., Ono, M., Kobayashi, M., Yamaguchi, S., Saito, K., Yamada, A., Fukumoto, S.

    Pediatric Dental Journal　26　(3)　2016

    DOI： 10.1016/j.pdj.2016.07.003 　

  34. Connexin 43 is necessary for salivary gland branching morphogenesis and FGF10-induced ERK1/2 phosphorylation Peer-reviewed

    Yamada, A., Futagi, M., Fukumoto, E., Saito, K., Yoshizaki, K., Ishikawa, M., Arakaki, M., Hino, R., Sugawara, Y., Ishikawa, M., Naruse, M., Miyazaki, K., Nakamura, T., Fukumoto, S.

    Journal of Biological Chemistry　291　(2)　904-912　2016

    DOI： 10.1074/jbc.M115.674663 　

  35. Plakophilin-1, a Novel Wnt Signaling Regulator, Is Critical for Tooth Development and Ameloblast Differentiation. Peer-reviewed

    Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I

    Plos One　11　(3)　e0152206　2016

    DOI： 10.1371/journal.pone.0152206 　

  36. Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation Peer-reviewed

    Liu, J., Saito, K., Maruya, Y., Nakamura, T., Yamada, A., Fukumoto, E., Ishikawa, M., Iwamoto, T., Miyazaki, K., Yoshizaki, K., Ge, L., Fukumoto, S.

    Scientific Reports　6　23670　2016

    DOI： 10.1038/srep23670 　

  37. Globoside accelerates the differentiation of dental epithelial cells into ameloblasts Peer-reviewed

    Nakamura, T., Chiba, Y., Naruse, M., Saito, K., Harada, H., Fukumoto, S.

    International Journal of Oral Science　8　(4)　205-212　2016

    DOI： 10.1038/ijos.2016.35 　

  38. 白血病治療を目指したヒト乳歯歯髄細胞から人工骨髄誘導法の開発

    山田 亜矢, 菊入 崇, 中村 卓史, 新垣 真紀子, 齋藤 幹, 福本 敏

    小児歯科学雑誌　53　(2)　220-220　2015/04

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

  39. Interaction between fibronectin and β1integrin is essential for tooth development Peer-reviewed

    Saito, K., Fukumoto, E., Yamada, A., Yuasa, K., Yoshizaki, K., Iwamoto, T., Saito, M., Nakamura, T., Fukumoto, S.

    PLoS ONE　10　(4)　0121667　2015

    DOI： 10.1371/journal.pone.0121667 　

  40. The enamel knot-like structure is eternally maintained in the apical bud of postnatal mouse incisors Peer-reviewed

    Nakatomi, C., Nakatomi, M., Saito, K., Harada, H., Ohshima, H.

    Archives of Oral Biology　60　(8)　1122-1130　2015

    DOI： 10.1016/j.archoralbio.2015.05.001 　

  41. Application of a tooth-surface coating material containing pre-reacted glass-ionomer fillers for caries prevention Peer-reviewed

    Suzuki, M., Yamada, A., Saito, K., Hino, R., Sugawara, Y., Ono, M., Naruse, M., Arakaki, M., Fukumoto, S.

    Pediatric Dental Journal　25　(3)　72-78　2015

    DOI： 10.1016/j.pdj.2015.08.003 　

  42. Molecular contribution to cleft palate production in cleft lip mice Peer-reviewed

    Sasaki, Y., Taya, Y., Saito, K., Fujita, K., Aoba, T., Fujiwara, T.

    Congenital Anomalies　54　(2)　94-99　2014

    DOI： 10.1111/cga.12038 　

  43. Establishment of ex vivo mucocele model using salivary gland organ culture Peer-reviewed

    Hino, R., Futagi, M., Yamada, A., Arakaki, M., Saito, K., Sugawara, Y., Ono, M., Fukumoto, E., Nakamura, T., Fukumoto, S.

    Pediatric Dental Journal　24　(2)　78-82　2014

    DOI： 10.1016/j.pdj.2014.03.003 　

  44. Evaluation of the optimal exposure settings for occlusal photography with digital cameras Peer-reviewed

    Sugawara, Y., Saito, K., Futaki, M., Naruse, M., Ono, M., Hino, R., Chiba, Y., Arakaki, M., Yamada, A., Fukumoto, S.

    Pediatric Dental Journal　24　(2)　89-96　2014

    DOI： 10.1016/j.pdj.2014.04.002 　

  45. Dynamics of bromodeoxyuridine label-retaining dental pulp cells during pulpal healing after cavity preparation in mice Peer-reviewed

    Saito K, Nakatomi M, Ohshima H

    Journal of endodontics　39　(10)　1250-1255　2013

    DOI： 10.1016/j.joen.2013.06.017 　

  46. Removable orthodontic appliance with nickel-titanium spring to reposition the upper incisors in an autistic patient Peer-reviewed

    Kan Saito, Insan Jang, Kazumi Kubota, Tomonori Hoshino, Hitoshi Hotokezaka, Noriaki Yoshida, Taku Fujiwara

    Special Care in Dentistry　33　(1)　35-39　2013/01

    DOI： 10.1111/j.1754-4505.2012.00291.x 　

    ISSN： 0275-1879 1754-4505

  47. Application of a tooth-surface coating material to teeth with discolored crowns Peer-reviewed

    Hashimura, T., Yamada, A., Iwamoto, T., Arakaki, M., Saito, K., Fukumoto, S.

    Pediatric Dental Journal　23　(1)　44-50　2013

    DOI： 10.1016/j.pdj.2013.03.011 　

  48. Establishment of crown-root domain borders in mouse incisor Peer-reviewed

    Juuri, E., Saito, K., Lefebvre, S., Michon, F.

    Gene Expression Patterns　13　(7)　255-264　2013

    DOI： 10.1016/j.gep.2013.05.001 　

  49. Traction of the lower second premolar by application of band-loop space maintainer in an autistic child Peer-reviewed

    Tomonori Hoshino, Kan Saito, Taku Fujiwara

    Pediatric Dental Journal　23　(2-3)　91-94　2013

    Publisher: Elsevier Ltd

    DOI： 10.1016/j.pdj.2013.05.001 　

    ISSN： 1880-3997 0917-2394

  50. Sox2+ Stem Cells Contribute to All Epithelial Lineages of the Tooth via Sfrp5+ Progenitors Peer-reviewed

    Emma Juuri, Kan Saito, Laura Ahtiainen, Kerstin Seidel, Mark Tummers, Konrad Hochedlinger, Ophir D. Klein, Irma Thesleff, Frederic Michon

    DEVELOPMENTAL CELL　23　(2)　317-328　2012/08

    DOI： 10.1016/j.devcel.2012.05.012 　

    ISSN： 1534-5807

  51. Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans Peer-reviewed

    Tomonori Hoshino, Yoshio Kondo, Kan Saito, Yutaka Terao, Nobuo Okahashi, Shigetada Kawabata, Taku Fujiwara

    CLINICAL AND VACCINE IMMUNOLOGY　18　(9)　1552-1561　2011/09

    DOI： 10.1128/CVI.05041-11 　

    ISSN： 1556-6811

  52. Phylogenetic analyses and detection of viridans streptococci based on sequences and denaturing gradient gel electrophoresis of the rod shape-determining protein gene Peer-reviewed

    Ikuri Konishi, Tomonori Hoshino, Yoshio Kondo, Kan Saito, Miyuki Nishiguchi, Kyoko Sato, Taku Fujiwara

    Journal of Oral Microbiology　1　(2009)　1-10　2009

    DOI： 10.3402/jom.v1i0.2015 　

    ISSN： 2000-2297

  53. Amelogenin binds to both heparan sulfate and bone morphogenetic protein 2 and pharmacologically suppresses the effect of noggin Peer-reviewed

    Kan Saito, Ikuri Konishi, Miyuki Nishiguchi, Tomonori Hoshino, Taku Fujiwara

    BONE　43　(2)　371-376　2008/08

    DOI： 10.1016/j.bone.2008.03.029 　

    ISSN： 8756-3282

  54. Amelogenin is a negative regulator of osteoclastogenesis via downregulation of RANKL, M-CSF and fibronectin expression in osteoblasts Peer-reviewed

    Miyuki Nishiguchi, Kenji Yuasa, Kan Saito, Emiko Fukumoto, Aya Yamada, Tomokazu Hasegawa, Keigo Yoshizaki, Yoko Kamasaki, Kazuaki Nonaka, Taku Fujiwara, Satoshi Fukumoto

    ARCHIVES OF ORAL BIOLOGY　52　(3)　237-243　2007/03

    DOI： 10.1016/j.archoralbio.2006.09.016 　

    ISSN： 0003-9969

  55. Infection-induced up-regulation of the costimulatory molecule 4-1BB in osteoblastic cells and its inhibitory effect on M-CSF/RANKL-induced in vitro osteoclastogenesis Peer-reviewed

    K Saito, N Ohara, H Hotokezaka, S Fukumoto, K Yuasa, M Naito, T Fujiwara, K Nakayama

    JOURNAL OF BIOLOGICAL CHEMISTRY　279　(14)　13555-13563　2004/04

    DOI： 10.1074/jbc.M303791200 　

    ISSN： 0021-9258

  56. Laminin alpha 2 is essential for odontoblast differentiation regulating dentin sialoprotein expression Peer-reviewed

    K Yuasa, S Fukumoto, Y Kamasaki, A Yamada, E Fukumoto, K Kanaoka, K Saito, H Harada, E Arikawa-Hirasawa, Y Miyagoe-Suzuki, S Takeda, K Okamoto, Y Kato, T Fujiwara

    JOURNAL OF BIOLOGICAL CHEMISTRY　279　(11)　10286-10292　2004/03

    DOI： 10.1074/jbc.M310013200 　

    ISSN： 0021-9258

  57. U0126 and PD98059, specific inhibitors of MEK, accelerate differentiation of RAW264.7 cells into osteoclast-like cells Peer-reviewed

    H Hotokezaka, E Sakai, K Kanaoka, K Saito, K Matsuo, H Kitaura, N Yoshida, K Nakayama

    JOURNAL OF BIOLOGICAL CHEMISTRY　277　(49)　47366-47372　2002/12

    DOI： 10.1074/jbc.M208284200 　

    ISSN： 0021-9258

  58. Cleft palate- and lip-related gene expression analyzed by cDNA microarray hybridization Peer-reviewed

    Kan Saito, Satoshi Fukumoto, Emiko Fukumoto, Michi-ichiro Itoh, Kenji Yuasa, Yuzo Kato, George Goto

    Pediatric Dental Journal　12　(1)　35-42　2002/03

    ISSN： 0917-2394

  59. 出力可変型キセノン照射器の光重合型レジンの物理的性質に及ぼす影響 Peer-reviewed

    釜崎陽子, 福本敏, 久保田一見, 吉田至純, 斎藤幹, 福本恵美子, 後藤譲治

    小児歯科学雑誌　39　(1)　146-158　2001/03

    Publisher: The Japanese Society of Pediatric Dentistry

    DOI： 10.11411/jspd1963.39.1_146 　

    ISSN： 0583-1199

    More details Close

    This study was undertaken to investigate the effect of the Xenon-lamp curing unit (Flipo) on the elemental properties of light curing resin composite by comparison with a halogen-lamp curing unit (XL).<BR>1) The shear bond strengths of resin composite to bovine dentin using each curing unit were measured. The adhesive system and resin composite used in this study were Clearfil Liner Bond II® and Clearfil AP-X® (shades: A 2 and A 4). The irradiation time of XL was 40 seconds as the control, and that of Flipo was 5 seconds (stepped output of 2 seconds at 50% maximal output followed by full output for 3 seconds). The result of the test showed that the shear bond strength produced by Flipo was about equal to that by XL for shade A 2, but for shade A 4 that produced by Flipo was significantly lower than that by XL (p<0.01). Using XL showed high bond strengths independent of shade.<BR>2) The knoop hardness of the resin composite was measured for XL for 40 seconds, Flipo for 5 seconds and Flipo for 10 seconds (2 seconds at 50% maximal output followed by full output for 8 seconds)using Clearfil AP-X® (shades: A 2 and A 4). The result showed that when the shade was A 2, the knoop hardness produced by Flipo for 5 seconds was about equal to that by XL for 40 seconds, and that of Flipo for 10 seconds was significantly higher than those two groups (p<0.01). For shade of A 4, the knoop hardness produced by Flipo for 10 seconds was about equal to that of XL for 40seconds, and that of Flipo for 5 seconds was significantly lower than those two(p<0.01).<BR>3) The dye penetration test was performed for cavities prepared on extracted human premolar and filled with resin under the light curing condition of XL for 40 seconds, Flipo for 5 seconds (two stepped output) and Flipo for 5 seconds of exposure at 100% intensity to evaluate marginal sealing and wall adaptation. The class V cavity with the base at depth of 1 mm in the dentin was prepared on the buccal surface. The group of XL for 40 seconds showed the best marginal sealing and wall adaptation, but when using Flipo at 100% intensity for 5 seconds, the dye penetration score was higher and both the incidence and width of contraction gap were significantly increased. The results of the present experiment proved that using the Xenon-lamp curing unit (Flipo) is effective for hardening a resin composite of shade A 2 less than 2.0 mm-deep with 1/6 of irradiation time needed for the halogen-lamp curing unit (XL). However, if the cavity is deeper than 2.0 mm, sufficient polymerization may not be obtained by using Flipo for 5 seconds, especially for shade A 4, and moreover, using Flipo for 5 seconds at 100% intensity may degrade the marginal seal effectiveness and wall adaptation.

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    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

  23. 東日本大震災の被災地域における自己記入型食事調査票を用いた間食指導について

    山田 亜矢, 日野 綾子, 千葉 雄太, 馬目 歩実, 齋藤 幹, 福本 敏

    小児歯科学雑誌　55　(1)　81-81　2017/02

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  24. エナメル質期齲蝕への対応

    小野真理子, 小山田優, 齋藤幹, 山田亜矢, 福本敏

    日本歯科評論　76　(9)　155-158　2016/09

  25. 文献と臨床の橋わたし エナメル質の性状と成熟 : 耐酸性の獲得と生活状況の記録

    福本 敏, 齋藤 幹, 日野 綾子, 山田 亜矢

    日本歯科評論　76　(8)　149-152　2016/08

    Publisher: ヒョーロン・パブリッシャーズ

    ISSN： 0289-0909

  26. エナメル質の性状と熟成

    福本敏, 齋藤幹, 日野綾子, 山田亜矢

    日本歯科評論　76　(8)　149-152　2016/08

  27. エナメル質形成不全症に対する対応法について

    福本敏, 山田亜矢, 齋藤幹, 新垣真紀子

    日本歯科評論　76　(7)　157-160　2016/07

  28. 子どものこころと身体を見守り支援する大規模データ収集とリスク予測モデル構築の試み

    西田佳世, 近藤好夫, 西俣はるか, 今村圭吾, 佐藤恭子, 日高聖, 釜崎陽子, 西口美由季, 星野倫範, 藤原卓, 齋藤幹, 山崎要一, 朝田芳信, 八若保孝, 苅部洋行, 新谷誠康, 早崎治明

    小児歯科学雑誌　54　(1)　155-155　2016/02/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  29. S‐PRGフィラーの研磨材としての評価

    小野真理子, 日野綾子, 新垣真紀子, 菅原優, 鈴木真夕, 千葉雄太, 汲川利華, 齋藤幹, 山田亜矢, 福本敏

    小児歯科学雑誌　54　(1)　56-56　2016/02/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

    eISSN： 2186-5078

  30. 当科における過剰歯に関しての臨床統計的検討

    成瀬 正啓, 菅原 優, 日野 綾子, 只木 麻友, 馬目 歩実, 山口 知子, 齋藤 幹, 山田 亜矢, 福本 敏

    小児歯科学雑誌　54　(1)　60-60　2016/02

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

  31. 【歯の細胞生物学】 エナメル芽細胞の分化制御機構

    中村 卓史, 成瀬 正啓, 齋藤 幹, 福本 敏

    腎と骨代謝　29　(1)　15-24　2016/01

    Publisher: (株)日本メディカルセンター

    DOI： 10.19020/J02201.2016149814 　

    ISSN： 0914-5265

  32. デンタルケアグッズの選び方について

    齋藤幹, 福本敏

    健　44　(12)　37-38　2016

  33. 発生から考えた歯の再生

    福本敏, 新垣真紀子, 山田亜矢, 齋藤幹, 中村卓史

    日本抗加齢医学会雑誌　11　(2)　188-193　2015/05

  34. 飲水時に引き起こされた乳歯の完全脱臼の2例

    千葉雄太, 齋藤幹, 成瀬正啓, 日野綾子, 山田亜矢, 福本敏

    小児歯科学雑誌　53　(1)　103-103　2015/02/25

    Publisher: (公社)日本小児歯科学会

    ISSN： 0583-1199

  35. 過去6年間の全身麻酔下歯科治療の実態調査

    西俣 はるか, 河井 真理, 近藤 好夫, 佐藤 恭子, 日高 聖, 齋藤 幹, 釜崎 陽子, 西口 美由季, 倉田 眞治, 星野 倫範, 鮎瀬 卓郎, 藤原 卓

    障害者歯科　35　(3)　324-324　2014/09

    Publisher: (一社)日本障害者歯科学会

    ISSN： 0913-1663

  36. 生後マウス切歯apical budにエナメル結節様構造が恒久的に維持されている(Enamel knot-like structure is eternally maintained in the apical bud of postnatal mouse incisors)

    中富 千尋, 中富 満城, 齋藤 幹, 原田 英光, 大島 勇人

    Journal of Oral Biosciences Supplement　2014　129-129　2014/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  37. 「新う蝕予防」― S-PRG 技術を応用した新しい齲蝕予防の概念―

    新垣真紀子, 山田亜矢, 齋藤 幹, 福本 敏

    小児歯科臨床　19　(8)　53-60　2014/08

  38. New insights into the functions of enamel matrices in calcified tissues Peer-reviewed

    Fukumoto, S., Nakamura, T., Yamada, A., Arakaki, M., Saito, K., Xu, J., Fukumoto, E., Yamada, Y.

    Japanese Dental Science Review　50　(2)　47-54　2014

    DOI： 10.1016/j.jdsr.2014.01.001 　

  39. The influence of Sox21 as a novel ameloblast marker on tooth germ differentiation

    SAITO Kan, YAMADA Aya, NAKAMURA Takashi, FUKUMOTO Satoshi

    51　(2)　158-158　2013/04/25

    ISSN： 0583-1199

  40. Analysis of Connexin43-FGF10 signaling on salivary gland development

    FUKUMOTO Satoshi, FUTAGI Masaharu, IWAMOTO Tsutomu, NAKAMURA Takashi, YAMADA Aya, SAITO Kan

    51　(2)　156-156　2013/04/25

    ISSN： 0583-1199

  41. Oral bacterial flora of the eight-month-old infants who consulted the mothers' class in our hospital

    NISHIMATA Haruka, SAITO Kan, HOSHINO Tomonori, HIDAKA Kiyoshi, SATO Kyoko, FUJIWARA Taku

    51　(2)　278-278　2013/04/25

    ISSN： 0583-1199

  42. Regulation of dental epithelial cell proliferation and differentiation by laminin

    OIKAWA Satoko, FUTAGI Masaharu, YAMADA Aya, ARAKAKI Makiko, MIYAMOTO Ryoko, SAITO Kan, NAKAMURA Takashi, FUKUMOTO Satoshi

    51　(2)　274-274　2013/04/25

    ISSN： 0583-1199

  43. フィンランドと日本の歯科事情の違い③

    佐藤恭子, 齋藤 幹, 西俣はるか, 藤原 卓

    小児歯科臨床　17　(6)　83-86　2012/06

  44. フィンランドと日本の歯科事情の違い②

    齋藤 幹, 佐藤恭子, 西俣はるか, 藤原 卓

    小児歯科臨床　17　(5)　79-82　2012/05

  45. 乳歯の残存期間に関する調査研究

    齋藤 幹, 釜崎 陽子, 日高 聖, 藤原 卓

    小児歯科学雑誌　50　(2)　266-266　2012/04/25

    ISSN： 0583-1199

  46. Early Childhood Caries 患児の口腔細菌叢の解析

    佐藤 恭子, 西俣 はるか, 齋藤 幹, 西口 美由季, 星野 倫範, 藤原 卓

    小児歯科学雑誌　50　(2)　290-290　2012/04/25

    ISSN： 0583-1199

  47. フィンランドと日本の歯科事情の違い①

    齋藤 幹, 佐藤恭子, 西俣はるか, 藤原 卓

    小児歯科臨床　17　(4)　63-66　2012/04

  48. The difference of Finland and Japanese dentistry circumstances (No.1)

    Kan Saito, Kyoko Sato, Haruka Nishimata, Taku Fujiwara

    17　(4)　63-66　2012

  49. The difference of Finland and Japanese dentistry circumstances (No.2)

    Kan Saito, Kyoko Sato, Haruka Nishimata, Taku Fujiwara

    17　(5)　79-82　2012

  50. Sox21は新規エナメル芽細胞マーカーである

    齋藤 幹, 藤原 卓

    小児歯科学雑誌　49　(4)　337-337　2011/10/25

    ISSN： 0583-1199

  51. アメロジェニンによる骨代謝への影響

    齋藤 幹

    小児歯科学雑誌　47　(4)　555-560　2009/10

    Publisher: Japanese Society of Pediatric Dentistry

    DOI： 10.11411/jspd.47.4_555 　

    ISSN： 0583-1199

    More details Close

    Amelogenin is a major enamel matrix protein associated with the enamel mineralization. Recently,it was suggested that amelogenin may affect osteoclast. In this study, the effects of amelogenin and amelogenin containing Enamel matrix derivative (EMD) on osteoclastogenesis and osteogenesis were investigated. In co-cultures with calvaria osteoblasts and purified bone marrow cells, amelogenin inhibited osteoclastogenesis. Amelogenin did not have on effect directly on osteoclasts but rather an action on osteoblasts. Amelogenin inhibited the expression of fibronectin lead to down regulate of nuclear factor κB ligand (RANKL) and macrophage-colony stimulating factor (M-CSF), result in osteoclastgenesis was inhibited. Furthermore, 2-Dimension PAGE of EMD followed by western blot analysis revealed that EMD contained amelogenin and BMP 2/4, but not TGF-β . Binding of amelogenin to BMP 2 through heparin/heparansulfate as well as noggin was confirmed in binding assay. Although amelogenin exhibits no noggin-like antagonistic properties against BMP, amelogenin inhibited the binding between BMP 2 and noggin and high density amelogenin reduced the inhibition of alkaline phosphatase activity by noggin. These results suggest that amelogenin effects on osteoblast not only suppressing osteoclast differentiation, but also reducing the inhibition of the BMP by noggin.

  52. Porphyromonas gingivalis の TPR domain protein (PG1385) 解析

    近藤 好夫, 星野 倫範, 齋藤 幹, 藤原 卓

    小児歯科学雑誌　47　(2)　396-396　2009/04/25

    ISSN： 0583-1199

  53. Streptococcus mutans グルコシルトランスフェラーゼの新規エピトープとこれにより誘導された抗血清による活性抑制効果について

    星野 倫範, 齋藤 幹, 藤原 卓

    小児歯科学雑誌　47　(2)　238-238　2009/04/25

    ISSN： 0583-1199

  54. CL/Fr口唇口蓋裂発症胎仔マウスの口蓋棚成長に対する分子関与

    佐々木康成, 田谷雄二, 齋藤幹, 藤田和也, 青葉孝昭, 藤原卓

    Journal of Oral Biosciences　51　(Supplement)　2009

    ISSN： 1349-0079

  55. 過去三年間の全身麻酔下歯科治療の動向調査

    星野 倫範, 久保田 一見, 釜崎 陽子, 西口 美由季, 齋藤 幹, 佐藤 恭子, 日高 聖, 小西 郁理, 近藤 好夫, 佐々木 康成, 細矢 由美子, 藤原 卓

    小児歯科学雑誌　46　(2)　257-257　2008/04/25

    ISSN： 0583-1199

  56. 哺乳齲蝕はなぜできるのか? : 糖原病I型患者の口腔細菌叢の解析から

    佐藤 恭子, 星野 倫範, 齋藤 幹, 西口 美由季, 小西 郁理, 藤原 卓

    小児歯科学雑誌　46　(2)　152-152　2008/04/25

    ISSN： 0583-1199

  57. 診療参加型実習にむけた小児歯科領域の実技実習への取り組み : 学生へのアンケートからの検討

    西口 美由季, 星野 倫範, 齋藤 幹, 佐藤 恭子, 釜崎 陽子, 佐々木 康成, 久保田 一見, 細矢 由美子, 藤原 卓

    小児歯科学雑誌　45　(2)　313-313　2007/05/25

    ISSN： 0583-1199

  58. プラークバイオフィルム中の Streptococcus mutans はラクトース分解酵素を産生するか?

    佐藤 恭子, 星野 倫範, 齋藤 幹, 西口 美由季, 小西 郁理, 近藤 好夫, 藤原 卓

    小児歯科学雑誌　45　(2)　221-221　2007/05/25

    ISSN： 0583-1199

  59. マウス口唇口蓋裂自然発症と口蓋棚間葉織の増殖との関係についての分子生物学的検索

    佐々木 康成, 齋藤 幹, 田谷 雄二, 青葉 孝昭, 藤原 卓

    小児歯科学雑誌　45　(2)　211-211　2007/05/25

    ISSN： 0583-1199

  60. ミュータンスレンサ球菌の定着による口腔細菌叢の推移とPCR法による確認

    小西 郁理, 星野 倫範, 出口 範子, 斎藤 幹, 藤原 卓

    小児歯科学雑誌　44　(2)　168-168　2006/04/25

    ISSN： 0583-1199

  61. ブタエナメル抽出物エムドゲインのプロテオーム解析

    斉藤 幹, 星野 倫範, 小西 郁理, 藤原 卓

    小児歯科学雑誌　44　(2)　237-237　2006/04/25

    ISSN： 0583-1199

  62. 歯原性上皮細胞分化におけるTGF-β1の役割

    小西 郁理, 福本 敏, 湯浅 健司, 釜崎 陽子, 斉藤 幹, 藤原 卓, 野中 和明

    小児歯科学雑誌　43　(2)　227-227　2005/04/25

    ISSN： 0583-1199

  63. アメロジェニンによるフィブロネクチンを介したRANKL,M-CSFの発現制御

    斎藤 幹, 西口 美由季, 湯浅 健司, 藤原 卓, 福本 敏

    Journal of oral biosciences　46　(5)　414-414　2004/09/01

    Publisher: 歯科基礎医学会

    ISSN： 1349-0079

  64. 歯原性上皮細胞における包括的増殖因子シグナルの解明

    小西 郁理, 湯浅 健司, 斎藤 幹, 釜崎 陽子, 山田 亜矢, 原田 英光, 藤原 卓, 福本 敏

    Journal of oral biosciences　46　(5)　413-413　2004/09/01

    Publisher: 歯科基礎医学会

    ISSN： 1349-0079

  65. エナメル蛋白によるエナメル質小柱構造決定の分子メカニズム

    釜崎 陽子, 福本 敏, 斉藤 幹, 山田 亜矢, 西口 美由季, 藤原 卓, 山田 吉彦

    小児歯科学雑誌　42　(2)　238-238　2004/04/25

    ISSN： 0583-1199

  66. アメロジェニンによる歯根吸収抑制メカニズムの解明

    西口 美由季, 福本 敏, 斉藤 幹, 湯浅 健司, 小西 郁理, 藤原 卓

    小児歯科学雑誌　42　(2)　237-237　2004/04/25

    ISSN： 0583-1199

  67. ラミニンα2の歯の発生における役割

    湯浅 健司, 福本 敏, 釜崎 陽子, 山田 亜矢, 福本 恵美子, 斉藤 幹, 岡元 邦彰, 藤原 卓, 加藤 有三

    歯科基礎医学会雑誌　45　(5)　290-290　2003/09/01

    Publisher: 歯科基礎医学会

    ISSN： 0385-0137

  68. アメロブラスチンの細胞接着ドメインの同定

    福本 敏, 福本 恵美子, 斉藤 幹, 山田 亜矢, 釜崎 陽子, 藤原 卓

    歯科基礎医学会雑誌　45　(5)　286-286　2003/09/01

    Publisher: 歯科基礎医学会

    ISSN： 0385-0137

  69. 感染骨芽細胞から産生される4-1BBの解析-各種細菌に対する応答性の違いと標的細胞の検討

    斉藤 幹, 大原 直也, 佛坂 斉祉, 福本 敏, 藤原 卓, 中山 浩次

    歯科基礎医学会雑誌　45　(5)　365-365　2003/09/01

    Publisher: 歯科基礎医学会

    ISSN： 0385-0137

  70. ダウン症候群由来細胞の炎症性サイトカインに対する応答性について

    湯浅 健司, 福本 敏, 斉藤 幹, 福本 恵美子, 長谷川 智一, 加藤 有三, 後藤 讓治

    小児歯科学雑誌　40　(2)　377-377　2002/04/25

    ISSN： 0583-1199

  71. 細菌感染により骨代謝へ影響を与える新たな分子の探索

    斉藤 幹, 福本 敏, 後藤 讓治

    小児歯科学雑誌　39　(2)　463-463　2001/04/01

    ISSN： 0583-1199

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Books and Other Publications 6

  1. 子どものお口の発育段階別で答える小児歯科のQ&A27 (デンタルダイヤモンド増刊号)

    浜野美幸, 岡暁子, 大川玲奈

    デンタルダイヤモンド社　2023/07/01

    ISBN: 4885105706

  2. 小児歯科学 第6版

    白川, 哲夫 (歯科), 福本, 敏, 岩本, 勉(歯科), 森川, 和政

    医歯薬出版　2023/02

    ISBN: 9784263458945

  3. 小児科外来や乳幼児健診で使える食と栄養相談Q&A 第二版

    平岩, 幹男, 大矢, 幸弘, 堤, ちはる, 渡部, 茂 (小児歯科学)

    診断と治療社　2022/12

    ISBN: 9784787825834

  4. COLOR ATLAS No.50 子どもの健やかなお口をつくる GPのための小児の歯科診療

    松風　2021/01

  5. 小児歯科学 第5版

    白川哲夫, 飯沼光生, 福本敏, 香西克之, 野中和明, 山座治義, 島村和宏, 長谷川信乃, 早﨑治明, 齊藤一誠, 山田亜矢, 山﨑要一, 武元嘉彦, 岩﨑智憲, 八若保孝, 大須賀直人, 仲野道代, 有田憲司, 苅部洋行, 宮新美智世, 岩本勉, 牧憲司, 佐伯桂, 藤田優子, 藤原卓, 星野倫範, 渡部茂, 木本茂成, 大久保孝一郎, 田村文誉, 小方清和, 新谷誠康, 仲野和彦, 齋藤幹, 佐々木康成

    2017/12

  6. 小児科外来や乳幼児健診で使える 食と栄養相談Q&A 第一版

    大矢, 幸弘, 堤, ちはる, 渡部, 茂, 平岩, 幹男

    診断と治療社　2016/05/16

    ISBN: 478782208X

Show all Show first 5

Research Projects 19

  1. 抗腫瘍を目指した新規石灰化誘導因子の同定と歯胚発生への役割解明

    齋藤 幹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(B)

    Category: 基盤研究(B)

    Institution: 東北大学

    2023/04/01 - 2026/03/31

  2. Identification of novel calcification-inducing factors for anti-tumor and investigation of their role in tooth development.

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2023/04 - 2026/03

  3. 細胞外環境制御による細胞運命決定にかかる細胞内シグナル作動機構の解明

    岩本 勉, 齋藤 幹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(B)

    Category: 基盤研究(B)

    Institution: 東京医科歯科大学

    2023/04 - 2026/03

  4. The tooth germ is formed utilizing induction of Epithelial to Mesenchymal Transition (EMT) from epithelium

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2018/04 - 2023/03

  5. Retrospective quantitative analysis of juvenile stress using dentin matrix

    YAMADA SATORU

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Challenging Research (Exploratory)

    Category: Challenging Research (Exploratory)

    Institution: Tohoku University

    2020/07 - 2022/03

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    Mice were intravenously administrated corticosterone at early childhood or juvenile stage and the amount of corticosterone in mineralized hard tissue of their teeth was quantified by ELISA method. The results revealed that corticosterone was detected in mineralized hard tissue of the teeth of corticosterone-injected group but not PBS-injected control group. This result suggested that systemically administrated corticosterone could be deposited into hard tissue of teeth. Corticosterone level in circulation of the mice received early life stress was significantly higher than that of control mice (p=0.0019). Corticosterone level in hard tissue of the teeth of adult mice that have experienced early life stress was 0.5343 pg/mg and that of control adult mice was 0.3547 mg/kg (p = 0.2046). These results indicated that corticosterone induced by early life stress at early childhood stage possibly accumulated in hard tissue of the teeth and corticosterone level was retained during the lifespan.

  6. Influence of chromosome 13 abnormality on hard tissue

    SAITO KAN

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04 - 2018/03

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    The osteoblasts were collected from Sox21KO and WT mouse, and the expression of the bone metabolism marker was investigated by RT-PCR. The decrease of expression level was not observed in the Sox21KO mouse. Next, the effect on osteoclasts was examined. The osteoclasts differentiation potency was compared by TRAP staining from the macrophage of spleen origin, and the difference was not found in this either. It was considered indirect effect through the other cells from these results. A blood was tested since Sox21 is expressed in the blood. No changes major in the item about the bone metabolism were identified. The osteoclast differentiation was induced from the bone marrow cell collected from the mouse femur because Sox21 was expressed in the bone marrow too. The number of osteoclasts in the Sox21KO Mouse increased compared with WT mouse.

  7. Regulation and determination of organ derived from invaginated epithelium

    FUKUMOTO SATOSHI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2014/04 - 2017/03

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    Tooth and hair are formed by invagination of epithelium. Invaginated epithelium interacts with neural crest derived mesenchymal tissues, and then formed tooth and hair. However, mechanism of organ determination has never clearly understood. Sox21 is one of the transcription factor, and expressed in dental epithelium. Sox21 knockout mice showed hair formation in incisor indicating that Sox21 may be involved in the determination of organs. Decrease Sox21 inhibited ameloblast marker gene expression, and induced hair marker genes including keratins. ChipSeq analysis showed that Sox21 directly binds to Anapc10 gene promoter region and regulated their expression in dental epithelium. In Sox21 knockout mice, Anapc10 expression was decreased in tooth germ. Further, Sox21 over expression induced Anapc10 expression. These results suggested that Sox21 regulates Anapc10 expression and organ determination.

  8. The metagenome analysis of salivary microflora in Japanese children with Early Childhood Caries and reassessment of causative bacteria and risk factors

    SATOH Kyoko, HOSHINO Tomonori, SAITOH Kan, NISHIGUCHI Miyuki, FUJIWARA Taku

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Nagasaki University

    2013/04 - 2016/03

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    We analyzed the salivary flora of children with Early Childhood Caries to reassess the causative bacteria and risk factors of Early Childhood Caries. Streptococcus mutans was not detected from two caries affected children, and Lactic acid bacteria such as the genus Lactococcus, Enterococcus, Lactbacillus, Bifidobacterium and Pediococcus were not detected in caries non affected and caries affected children. As the results of metagenome analysis of salivary microflora, the average detection ratio of the genus Streptococcus, Abiotrophia, Neisseria, Veillonella, Rothia, Lautropia, Actinomyces, Prevotella, Gemalla and Porphyromonas in caries active children would differ from that of caries free children. In the microorganism community analysis, it was suggested that the composition of salivary bacterial flora would be changed by having caries or not.

  9. Collection and Construction of database for physiological and physical support of children

    FUJIWARA Taku, HOSHINO Tomonori, NISHIGUCHI Miyuki, HIDAKA Kiyoshi, ASADA Yoshinobu, YAMASAKI Youichi, KAMASAKI Youko, MOTOMURA Youichi, SAITOH Kan, KARIBE Hiroyuki, YAWAKA Yasutaka, HAYASAKI Haruaki, SATOH Kyouko, SHINTANI Seikou, HIRATA Souichirou

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Nagasaki University

    2012/04 - 2016/03

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    The purpose of this study is to analyze the data of patients’ background using machine learning method and to develop an application that could output helpful information. We also tried to construct a new system that will enable to continuously collect and utilize these data using tablet. We included data collection system of dental trauma. Data collection system was completed. We will continue data collection using this system. We tried to make decision tree from questionnaire of the mother class using application Weka (Machine Learning Group at the University of Waikato, New Zealand) . Several decision trees were generated, but we could not obtain proper decision trees according with the conventional theory. Since the contents of the sheet were slightly different at each clinic, missing values were likely to influence the analysis. A unified questionnaires style would be necessary. In addition, selection of the objective variable should be more important.

  10. Novel ameloblast marker Sox21 regulate the differentiation of dental epithelial cells?

    SAITO Kan

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2012/04 - 2015/03

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    Sox21 which is a novel mature ameloblast marker was related to the differentiation to terminal ameloblasts from mature ameloblasts. Therefore, The cell polarity of a mature ameloblast disappears by absence of Sox21. For this reason, since a formation of an enamel rod is impeded, the abnormals in a crystal structure and hypomineralization of an enamel rod are triggered. Finally, The defect of Sox21 causes an amelogenesis imperfecta. Moreover, the expression of Sox21 in an ameloblast was regulated by Shh.

  11. マウス口唇裂発症に続く口蓋裂発症機序解明と口蓋裂レスキュー

    佐々木 康成, 藤原 卓, 田谷 雄二, 星野 倫範, 釜崎 陽子, 日高 聖

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 長崎大学

    2009 - 2011

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    過去のマイクロアレイ分析より,自然発症口唇口蓋裂マウスであるCL/Frの罹患胎児の口蓋棚mRNA発現量が,正常胎児と比較して1/2以下に小さい131分子および2倍以上に多い112分子が同定された。そこで,口蓋裂発症との関連分子を明らかにするために,マイクロアレイのデータ解析を進め,Normalized valueが罹患胎児および正常胎児とも1以上のもの,およびcell cycle,cell deathあるいはtranscriptionなど,GO分類で細胞増殖と何らか関係が報告されている口蓋裂発症候補分子をしぼり込んだ。これらの条件を満たす分子として,9分子(cell cycle関連Frk,Nek2,Katnb1,Tacc1,Transcription関連:Tox,Hmg20a,Usfl,cell death関連:Fastkd2,Dnase2a)が同定された。さらにこの9分子について,リアルタイムRT-PCRを用いて,口唇口蓋裂罹胎児および正常胎児の口蓋棚挙上期の口蓋棚mRNA詳細に比較検討した結果,Nek2,Katnb1,Tacc1,Hmg20a,Usf1,Fastkd2の6分子が,マイクロアレイ解析の大小関係と一致して,CL/Fr口蓋裂発症治胎児が正常胎児と比較して有意に大きいものであった。これらの分子は,神経細胞への分化を促進するもの,癌細胞において発現量が増加するもの,あるいはミトコンドリア症と関連するものなど,いずれも口蓋棚の正常な成長に対してはネガティブ因子であった。口唇裂に引き続き発生する口蓋裂のメカニズムは,従来,口唇裂に引き続き起きる2次的なもの以外には考えられていなかったが,本研究から,同定された一つ一つの分子が相互に口蓋棚の成長に働き,口蓋棚の細胞増殖を抑制し口蓋裂の発生につながっていることが明らかとなり、口蓋裂の成長メカニズムの解明および診断や治療のための重要な基礎的データが得られた。

  12. Exhaustive analysis using DGGE method for oral flora causing the infective endocarditis

    NISHIGUCHI Miyuki, FUJIWARA Taku, HOSHINO Tomonori, KAMASAKI Yoko, SAITO Kan

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Nagasaki University

    2009 - 2011

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    We established DGGE method based on the variety of the Rod shape-determining protein gene(rodA) as a target the oral streptococcus which was the maximal bacterial species in the oral cavity causing infective endocarditis(IE). Based on this, we performed exhaustive analysis of the bacterial flora as saliva specimen. Also, we estimated bacterial strain from an examination for biochemical property and a 16S rRNA gene of the strain from a patient who has contracted IE. We determined a draft sequence of the genomic DNA of strain identified with Streptococcus mutans and performed comparison between genomic sequence that was already presented on a database. then, we reviewed the pathogenesis factor that was specific for IE. We made the study of bacterial species associated with dental caries, a periodontal disease-causing bacteria, the investigation of infecting organism for opportunistic infections by using an examination for dental caries, an examination for periodontal disease, fence-sitting bacteria examination of BML company.

  13. Determine the novel amelogenin receptor which influence bone formation

    KONISHI Ikuri, HOSHINO Tomonori, NISHIGUCHI Miyuki, HIDAKA Kiyoshi, FUJIWARA Taku, SATOU Kyouko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Nagasaki University

    2009 - 2011

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    Amelogenin stimulated about 23 kinds transcription factors in osteoblast. These transcription factors were analyzed by in silico data mining. This result was speculated that Amelogenin signaling pathways were ERK signal, Wnt/β-catenin signal and TGF-βBMP/ Smad signal. To confirm this result, it was inspected by a phosphorylation antibody whether Amelogenin through these signals in osteoblast or not. Smad2/ 3/ 5 did not effect but ERK andβ-catenin were activated by amelogenin. Thus, it is suggested that novel amelogenin receptor not involving LAMP1 and CD63 may be Frizzled families which were a Wnt receptor.

  14. エナメルマトリックス蛋白による骨代謝への影響 Competitive

    System: 科学研究費補助金

    2007 - 2009

  15. Study on cleft palate morphogenesis in spontaneous cleft lip and palate

    SASAKI Yasunori, FUJIWARA Taku, HOSHINO Tomonori, SAITO Kan, KAMASAKI Youko, TAYA Yuji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Nagasaki University

    2007 - 2008

  16. Establishment of the juvenile periodontitis therapy by the interaction of amelogenin and BMP

    SAITO Kan

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Nagasaki University

    2007 - 2008

  17. Resolution of quorum-sensing systems in dental plaque biofilm and development of the method that prevents the plaque formation by the control of them

    HOSHINO Tomonori, FUJIWARA Taku, KAN Saito

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Nagasaki University

    2006 - 2007

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    Dental plaque is the biofilm which is formed on the tooth surface, and is a protective barrier for the bacteria against the change of the environment, the antibiotic, and the host immune response. First this biofilm is formed by the extracellular polysaccharide(glucan), which the mitis group streptococci produce from sugar, and then is reinforced by the adhesive and water-insoluble glucan, which the mutans group streptococci produce. Dental plaque biofilm plays an important role in the caries formation. In this biofilm, the plaque bacteria communicate with other bacteria by the auto-inducer like hormone. By this communication system that is commonly known as quorum-sensing, the bacterial population density in the dental plaque is thought to be kept constant, and the bacterial mass against the host immune system is constructed. In this study, we analyzed gtfB, gtfC, gtfD, dexA, and b-galactosidase genes, using real-time RT-PCR based on the comparative CT method and demonstrated that three gtf and β-galactosidase genes were suppressed; however, dexA was upregulated. Thus, S. mutans could control biofilm mass by balancing glucan production and hydrolysis. Such control is necessary to maintain colonization on a smooth surface and to ensure that external carbohydrate present across the glucan layer is not used. On the other hand, the epitope assay of glycosyltransferase was performed and the anti-glycosyltransferase DNA vaccines were developed. Moreover, the change of the secretory IgA Secretion and the bacterial numbers in saliva by menstruation cycle were analyzed.

  18. Inhibition of S.mutans colonization by the DNA vaccine targeted on GTFC and It's anti-cariogenic effects

    FUJIWARA Taku, HOSHINO Tomonori, SASAKI Yasunori, SATOH Kyoko, SAITOH Kan

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Nagasaki University Graduate School of Biomedical Sciences

    2005 - 2006

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    DNA vaccine can induce the immune response by the antigen protein which expressed in situ by the expression vector carrying the antigen gene. Since DNA vaccine is stable and cheap, it has been used to various fields. Streptococcus mutans, which is cariogenic bacterium, possesses three glucosyltransferases (GTFs). These are GTFB, GTFC, and GTFD. Since GTFB possesses the most strong activity of insoluble glucan synthesis, it has been thought as the most important factor of the colonization of S.mutans. Recently, we revealed that GTFC related the first step of the adhesion. Therefore, the inhibition of the activity of GTFC should decrease the cariogenic biofilm formation. 1. Generation of DNA vaccines targeting N-terminal region The specific region in the N-terminal of GTFB and GTFC was selected as the target of DNA vaccine. This region was cloned into a plasmid vector pSecTag2B, then into an adenovirus vector, generating the anti caries DNA vaccine. The antigenic protein was detected by SDS-PAGE and Western blot. The stability and amount of antigen expression by GTFC was better than that of GTFB. 2. Immunization of DNA vaccine to mouse DNA vaccines against GTFB and GTFC were injected to the mice, and antibody titer was determined by ELISA. The immune response against GTFC was greater than that of GTFB. Western blot with the antiserum revealed that both sera were specific to GTFB and GTFC, respectively.

  19. 若年性歯周炎における骨芽細胞を介する重度骨破壊の分子メカニズムの解明

    齋藤 幹

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 若手研究(B)

    Category: 若手研究(B)

    Institution: 長崎大学

    2005 - 2006

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    以前の研究では骨芽細胞にBCGやEscherichia coli等を感染させる事により、4-1BBの増殖が認められた。更にLPSでも4-1BBの昇が認められた事から、免疫反応により4-1BBの発現が上昇すると考えたれていた。今回の研究では以前の研究と同じ手法を用い、若年性歯周炎の原因菌であるA.actinomycetemcomitans(A.a)をマウス骨芽細胞様細胞MC3T3-E1細胞に感染させた。0,6,12,24時間後にPBSにて洗浄し、細胞外の細菌を除去したのち、Trizolを用いて回収した細胞からRT-PCRを行い4-1BBの発現量について検討した。その結果、A.aでは4-1BBの発現量が減少した。一方、Salmonella typhimuriumや、Staphylococcus aureusを骨芽細胞に感染させた場合、4-1BBの上昇が認められた事から、菌種によって4-1BBの発現に違いがある事が判明した。今回、A.aでは4-1BB発現が増加しなかった理由として、A.aはロイコトキシンを有し、白血球や単球に作用して溶血させる白血球抵抗作用があり、また4-1BBはT細胞の副刺激分子として知られており、免疫活性時に発現する事から、今回A.aが有するロイコトキシンによって細胞の免疫活性が低下したため、発現が低下したのではないかと考えられる。 そこで、免疫系に関与する細胞が4-1BBによる破骨細胞抑制効果に影響を与える可能性について検討するべくB細胞、T細胞を有しないSCIDマウスの骨髄細胞を使って破骨細胞誘導実験を行った。その結果、SCIDマウスの場合でも4-1BBによる破骨細胞分化抑制が認められた事から、4-1BBは恐らく単球・マクロファージに直接作用して破骨細胞の分化抑制をしていると考えられる。

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Works 3

  1. 新規エナメル芽細胞マーカーＳｏｘ２１は歯原性上皮細胞の分化を調節するのか？

    2012 - 2015

  2. アメロジェニンとBMPの相互作用による若年性歯周炎治療法の確立

    2007 - 2009

  3. 若年性歯周炎における骨芽細胞を介する重度骨破壊の分子メカニズムの解明

    2005 - 2007