Details of the Researcher

PHOTO

Yuki Kajita
Section
Graduate School of Medicine
Job title
Research Associate
Degree

  • 博士（医学）（群馬大学）

  • 修士（生命医科学）（群馬大学）

Research History 2

  • 2016/05 - Present
    Tohoku University

  • 2016/04 - 2016/05
    Tohoku University

Education 2

  • Gunma University　Faculty of Medicine

    2010 - 2016

  • Waseda University　School of Human Sciences

    2006 - 2010

Committee Memberships 1

  • NeuroMarkers（KeAi Publishing / Elsevier）　Youth Editorial Board Member

    2025/07 - 2026/08

Professional Memberships 4

  • 日本動物学会

    - Present

  • THE PHYSIOLOGICAL SOCIETY OF JAPAN

  • THE JAPANESE SOCIETY FOR NEUROCHEMISTRY

  • THE JAPAN NEUROSCIENCE SOCIETY

Research Interests 4

  • Neural development in early life

  • 行動実験

  • 成体神経新生

  • 精神疾患

Research Areas 3

  • Life sciences / Animals: biochemistry, physiology, behavioral science /

  • Life sciences / Laboratory animal science /

  • Life sciences / Neuroscience - general /

Awards 3

  1. 国際学会派遣支援助成

    2015　群馬大学

  2. ISNトラベルアワード

    2015　日本神経化学会

  3. ISN Special conference travel award for the 6th Special conference of the International Society of Neurochemistry

    2014　International Society of Neurochemistry

Papers 8

  1. Maternal separation after postnatal day 10 induces increase in depression-like behavior with decrease in hippocampal dendritic spines, but no change in anxiety-like behavior in male rats International-journal Peer-reviewed

    Kento Takabayashi, Yuki Kajita*, Hajime Mushiake, *KT and YK contributed equally to this work

    Behavioural Brain Research　115617-115617　2025/05

    Publisher: Elsevier BV

    DOI： 10.1016/j.bbr.2025.115617 　

    ISSN： 0166-4328

  2. Pterostilben upregulates GAD67-mediated GABA synthesis in hippocampal parvalbumin-positive cells International-journal Peer-reviewed

    Yuki Kajita, Ko Ono, Saya Kaneda, Hajime Mushiake

    Neuroscience　573　482-490　2025/05

    Publisher: Elsevier BV

    DOI： 10.1016/j.neuroscience.2025.03.060 　

    ISSN： 0306-4522

  3. Dynamic changes in seizure state and anxiety-like behaviors during pentylenetetrazole kindling in rats. International-journal Peer-reviewed

    Yuki Kajita, Hajime Mushiake

    Epilepsy & behavior : E&B　159　110019-110019　2024/08/29

    Publisher:

    DOI： 10.1016/j.yebeh.2024.110019 　

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    INTRODUCTION: Excessive anxiety is a mental disorder, and its treatment involves the use of benzodiazepines, a class of drugs that enhance the effects of the neurotransmitter gamma-aminobutyric acid (GABA) at the GABAA receptor. Anxiety disorders are frequent comorbidities in patients with epilepsy, and it has been speculated that anxiety disorders and epileptic seizures share common neurobiological mechanisms. However, conflicting results regarding anxiolytic and anxiogenic effects have been reported in animal models of epilepsy induced by pentylenetetrazole (PTZ) injections, and the causes of this discrepancy are unknown. We hypothesized that anxiety-like behaviors would change dynamically according to the changes in epilepsy susceptibility that occur during the PTZ kindling process. Therefore, we attempted to change anxiety-like behaviors bidirectionally depending on the number of PTZ injections. METHODS: Adult male rats were injected with PTZ 20 times every other day, and stages of seizure onset were classified according to the Racine staging system. Anxiety-like behaviors were measured after 10 and 20 injections. The control group was injected with an equal volume of saline solution. Anxiety-like behaviors were investigated using the open-field, light/dark transition, elevated plus maze, and social interaction tests. RESULTS: Bimodal changes in seizure stage were observed in response to PTZ kindling. The increase in the seizure stage was transiently suppressed after 10 injections, and this decrease in epileptic sensitivity disappeared after 20 injections. However, none of the rats reached a fully kindled state after 20 PTZ injections. After 10 PTZ injections, anxiety-like behaviors decreased compared with those of the control group in the open field, light/dark transition, and elevated plus-maze tests. The anxiolytic effects correlated with the seizure stage in individual rats. After 20 PTZ injections, anxiety-like behaviors returned to control levels. CONCLUSION: PTZ kindling induced bimodal changes in the seizure stage. Anxiety-like behaviors decreased with transient decrease in epileptic sensitivity and returned to control levels with the disappearance of these states. These findings suggest a common neurobiological mechanism underlying anxiety disorders and epileptic seizures. In addition, the discrepancy in the previous studies, in which anxiety levels increase or decrease in PTZ-kindled animals, may be due to examination at different phases of the kindling process.

  4. Pentylenetetrazole kindling induces dynamic changes in GAD65 expression in hippocampal somatostatin interneurons International-journal Peer-reviewed

    Yuki Kajita, Yuki Fukuda, Riho Kawamatsu, Takanori Oyanagi, Hajime Mushiake

    Pharmacology Biochemistry and Behavior　239　2024/03

    Publisher:

    DOI： 10.1016/j.pbb.2024.173755 　

    ISSN： 0091-3057

    eISSN： 1873-5177

  5. A lack of drebrin causes olfactory impairment. Peer-reviewed

    Yuki Kajita, Nobuhiko Kojima, Tomoaki Shirao

    Brain and Behavior　14　(1)　e3354　2023/12

    Publisher:

    DOI： 10.1002/brb3.3354 　

    eISSN： 2162-3279

  6. Heterogeneous GAD65 Expression in Subtypes of GABAergic Neurons Across Layers of the Cerebral Cortex and Hippocampus. International-journal Peer-reviewed

    Yuki Kajita, Hajime Mushiake

    Frontiers in behavioral neuroscience　15　750869-750869　2021

    Publisher:

    DOI： 10.3389/fnbeh.2021.750869 　

    ISSN： 1662-5153

  7. CRISPR/Cas9-engineered Gad1 elimination in rats leads to complex behavioral changes: implications for schizophrenia. International-journal Peer-reviewed

    Kazuyuki Fujihara, Kazuo Yamada, Yukio Ichitani, Toshikazu Kakizaki, Weiru Jiang, Shigeo Miyata, Takashi Suto, Daiki Kato, Shigeru Saito, Masahiko Watanabe, Yuki Kajita, Tomokazu Ohshiro, Hajime Mushiake, Yoshiki Miyasaka, Tomoji Mashimo, Hiroki Yasuda, Yuchio Yanagawa

    Translational psychiatry　10　(1)　426-426　2020/12/08

    Publisher:

    DOI： 10.1038/s41398-020-01108-6 　

    eISSN： 2158-3188

  8. Drebrin E regulates neuroblast proliferation and chain migration in the adult brain International-journal Peer-reviewed

    Yuki Kajita, Nobuhiko Kojima, Noriko Koganezawa, Hiroyuki Yamazaki, Kenji Sakimura, Tomoaki Shirao

    European Journal of Neuroscience　46　(6)　2214-2228　2017/09/01

    Publisher:

    DOI： 10.1111/ejn.13668 　

    ISSN： 1460-9568 0953-816X

    eISSN： 1460-9568

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Misc. 12

  1. Validation and optimization of quantitative activity-induced manganese-enhanced MRI.

    関本麻衣, 長谷川直樹, 谷平大樹, 菊田里美, 佐野裕美, 南部篤, 梶田裕貴, 大城朝一, 本間経康, 虫明元, 上村優輝, 松下知佳, 小山内実

    電気学会電子・情報・システム部門大会(Web)　2024　2024

  2. てんかんモデルラットにおけるGABA合成酵素の発現量の変化

    福田 湧希, 大柳 貴紀, 川松 里穂, 阿部 聡太, 梶田 裕貴, 虫明 元

    日本生理学雑誌　82　(2)　28-28　2020/05

    Publisher: (一社)日本生理学会

    ISSN： 0031-9341

  3. Loss of drebrin from dendritic spines in hippocampal neurons from Alzheimer's disease model mouse

    Koganezawa Noriko, Kajita Yuki, Yamazaki Hiroyuki, Saito Takashi, Sekino Yuko, Saido Takaomi C, Shirao Tomoaki

    Proceedings for Annual Meeting of The Japanese Pharmacological Society　93　2-P-135　2020

    Publisher: Japanese Pharmacological Society

    DOI： 10.1254/jpssuppl.93.0_2-p-135 　

    eISSN： 2435-4953

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    Alzheimer's disease (AD) is one of neurodegenerative diseases and the most common cause of dementia. Among pathology of AD, synaptic dysfunction has most correlation with cognitive dysfunction. Drebrin is an actin binding protein and stabilizes actin filaments. Drebrin-decorated stable actin filaments accumulate in dendritic spines and are thought to be crucial for synaptic plasticity but drebrin has been decreased at onset of dementia in AD. We therefore hypothesized that loss of drebrin, that is, loss of stable actin filaments from dendritic spines elicits synaptic dysfunction and causes dementia in AD. Here we used the App knock-in mouse model of AD (AppNL-G-F mouse), to analyze the details of abnormal synapse in AD. First we performed immunohistochemical analysis using AppNL-G-F mice brains. We focused on the cortex and found no drebrin immunoreactivity around amyloid plaques in the AppNL-G-F mice brains. We further used primary hippocampal cultured neurons derived from the AppNL-G-F mice (AppNL-G-F neurons) and evaluated synaptic status based on drebrin cluster number using high-content imaging analysis. Our data showed AppNL-G-F neurons had less drebrin clusters indicating low functionality of synapse. These data suggest that the loss of drebrin from the dendritic spine in AD brains causes synaptic dysfunction.

  4. GAD67ノックアウトラットの認知機能障害

    藤原和之, 藤原和之, 柿崎利和, JIANG Weiru, 宮田茂雄, 宮田茂雄, 須藤貴史, 齋藤繁, 山田一夫, 一谷幸男, 梶田裕貴, 大城朝一, 虫明元, 渡邉雅彦, 宮坂佳樹, 真下知士, 安田浩樹

    日本生物学的精神医学会(Web)　41st　2019

  5. ドレブリンEは成体脳において神経新生を促進する

    梶田 裕貴, 小金澤 紀子, 児島 伸彦, 崎村 建司, 白尾 智明

    日本生理学雑誌　79　(1)　3-3　2017/02

    Publisher: (一社)日本生理学会

    ISSN： 0031-9341

  6. Transient effect of X-irradiation and carbon ion-irradiation on synaptic function

    A. Puspitasari, N. Koganezawa, Y. Kajita, T. Shirao

    JOURNAL OF NEUROCHEMISTRY　134　307-307　2015/08

    ISSN： 0022-3042

    eISSN： 1471-4159

  7. Drebrin knockout results in the impairment of olfaction and adult neurogenesis

    Y. Kajita, N. Koganezawa, T. Shirao

    JOURNAL OF NEUROCHEMISTRY　134　180-181　2015/08

    ISSN： 0022-3042

    eISSN： 1471-4159

  8. B-34 マーモセット脳におけるシナプス可塑性関連蛋白ドレブリンの免疫組織化学的解析

    白尾 智明, 児島 伸彦, 梶田 裕貴

    霊長類研究所年報　43　102-102　2013/11/13

    Publisher: 京都大学霊長類研究所

    ISSN： 0286-4568

  9. B-7 マーモセット脳におけるシナプス可塑性関連蛋白ドレブリンの免疫組織化学的解析

    白尾 智明, 児島 伸彦, 梶田 裕貴

    霊長類研究所年報　42　102-102　2012/10/04

    Publisher: 京都大学霊長類研究所

    ISSN： 0286-4568

  10. マーモセット脳におけるドレブリンの免疫組織化学的解析

    梶田 裕貴, 三輪 美樹, 児島 伸彦, 中村 克樹, 白尾 智明

    The Kitakanto Medical Journal　61　(3)　454-454　2011/08

    Publisher: 北関東医学会

    ISSN： 1343-2826

    eISSN： 1881-1191

  11. ドレブリンノックアウトマウスを用いた海馬成体新生ニューロンの解析

    梶田 裕貴, 白尾 智明

    神経組織の成長・再生・移植研究会学術集会プログラム・予稿集　26回　32-32　2011/06

    Publisher: 神経組織の成長・再生・移植研究会

  12. Newly generated neurons are decreased in the adult hippocampus of drebrin-null mutant mice

    Yuki Kajita, Nobuhiko Kojima, Tomoaki Shirao

    Neuroscience Research　71　241　2011

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Presentations 26

  1. Temporal and sex-specific effects of childhood stress on depression-like behavior in adolescence

    Yuki Kajita, Kento Takabayashi, Hajime Mushiake

    2023/08

  2. 幼児期ストレスの時期特異性

    高林健人, 梶田裕貴, 虫明元

    第93回日本動物学会 早稲田大会　2022/09

  3. The increase of GAD65 expression by PTZ stimulations occurs specifically in SOM-positive interneurons

    Yuki Kajita, Yuki Fukuda, Riho Kawamatsu, Takanori Oyanagi, Hajime Mushiake

    2022

  4. Dynamic changes in GAD65 expression in PTZ kindling rats

    Yuki Fukuda, Takanori Oyanagi, Riho Kawamatsu, Yuki Kajita, Hajime Mushiake

    2021/03

  5. Dynamic changes in GAD65 expression in somatostatin-positive interneurons during the acquisition of generalized epileptic seizures

    Yuki Kajita, Yuki Fukuda, Riho Kawamatsu, Hajime Mushikae

    2020/07

  6. The regulation of adult neurogenesis in hippocampus by chronic fluoxetine treatment via parvalbumin-positive interneurons Invited

    2020/07

  7. てんかんモデルラットにおけるGABA合成酵素の発現量の変化

    福田 湧希, 大柳 貴紀, 川松 里穂, 阿部 聡太, 梶田 裕貴, 虫明 元

    第５１回 東北生理談話会　2019/11

  8. The GAD65-expression in interneuron subtypes in normal and seizure-prone hippocampus.

    Yuki Kajita, Yuki Fukuda, Takanori Oyanagi, Hajime Mushiake

    Neuro2019　2019/07

  9. マウス生体脳の新生ニューロンと行動におけるドレブリンの役割

    梶田裕貴

    第１回ドレブリン研究会　2019/03/08

  10. GAD65発現量を指標としたPTZキンドリング獲得におけるGABA制御機構の変化

    福田湧希, 梶田裕貴, 虫明元

    第12回リトリート大学院生研究発表会　2019

  11. 成体海馬の神経新生に対するGABA合成酵素の関与

    阿部聡太, 梶田裕貴, 虫明元

    第12回リトリート大学院生研究発表会　2019

  12. Drebrin E regulates neuroblast proliferation and chain migration in the adult brain. Invited

    ISN-APSN-JSN Advanced School　2017

  13. ドレブリン E は成体脳において神経新生を促進する

    Yuki Kajita

    東北生理談話会　2016

  14. Impairment olfaction and adult neurogenesis in drebrin knockout mouse

    2015

  15. Drebrin knockout mice show the impairment of olfaction and adult neurogenesis

    第38回日本神経科学大会　2015

  16. Drebrin knockout results in the impairment of olfaction and adult neurogenesis

    25th Meeting of the International Society for Neurochemistry　2015

  17. Drebrin knockout mice show olfactory dysfunction by impairment of adult neurogenesis and cell survival

    KAJITA Yuuki

    2015

  18. Drebrin knockout mice show the olfaction impairment caused by delayed neural exchange in olfactory bulb

    2014

  19. Drebrin A overexpression causes structural change of microtubule via F-actin modification in cultured fibroblast Invited

    The 6th ISN Special Conference　2014

  20. Drebrin knockout mice show the impairment of olfactory acuity via affecting adult neurogenesis

    2013

  21. Impairment of Adult Neurogenesis by Drebrin Knockout Results in the Olfactory Dysfunction

    KAJITA Yuuki

    the 2013 International Conference on Brain and Health Informatics　2013

  22. Differential role of drebrin in neuronal cell proliferation and migration in adult neurogenesis

    2012

  23. ドレブリンノックアウトマウスを用いた海馬成体新生ニューロンの解析

    第 26 回神経組織の成長・再生・移植研究会学術集会　2011

  24. Newly-generated neurons are decreased in the adult hippocampus of drebrin-null mutant mice

    2011

  25. マーモセット脳におけるドレブリンの免疫組織化学的解析

    第 58 回北関東医学会総会　2011

  26. ドレブリンノックアウトマウスを用いた成体新生ニューロンの解析

    第 2 回国際放射線神経性物学会大会　2011

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Research Projects 2

  1. GABAアンタゴニスト投与による抗不安作用の検証

    梶田 裕貴

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 若手研究

    Institution: 東北大学

    2020/04 - 2024/03

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    2年目は「短期と長期の痙攣誘発刺激が引き起こす不安様行動変化」を明らかにするため行動試験を中心に研究を行った。 ＧＡＢＡＡ受容体アンタゴニスト、ペンチレンテトラゾール（ＰＴＺ)の投与（１回/日で隔日投与、４０ mg/kg）１０回目ではラットの痙攣ステージはラシーンの分類に当てはめると大体２－３（頭部や四肢の痙攣）程度であった。しかし、殆どの個体が３－５回投与の時点で、ステージ４－５の重度の痙攣を起こし、その後、６-１０回目にかけて、徐々に減弱していくという過程を示した。ＰＴＺ１０回投与から１週間後に、この動物に対して、オープンフィールド試験、明暗箱試験、高架式十字迷路試験を実施したところ、生理食塩水投与（ＣＴＬ）群に比べ、不安様行動の低下が確認された。行動試験後、同個体に対して、ＰＴＺ注射を更に繰り返すと、２０回投与の時点で、多くの個体がステージ４－５の痙攣を繰り返し起こすことが確認された。ＰＴＺ２０回投与から１週間後、同様の行動試験を行ったところ、不安様行動の低下は見られず、ＣＴＬ群と同程度の不安レベルを示した。また、ＰＴＺ１０回投与後の抗不安作用が、より低濃度のＰＴＺ投与でも得られるかを検証する為、４ mg/kgの量で実験を行った。この実験ではラットに痙攣発作は見られず、１０回投与後の不安関連行動にもＣＴＬと有意な差は見られなかった。 今回の結果から痙攣誘発刺激は短期でのみ、不安様行動を減少することが明らかになった。また、この抗不安作用には痙攣発作が引き金となっている可能性が考えられる。

  2. Unvealing a functional implication of beta oscillations in the motor cortex

    Watanabe Hidenori, MUSHIAKE Hajime, TAKAHASHI Kazutaka, KAJITA Yuki, Nambu Atsushi, CHIKEN Satomi, SANO Hiromi, KOBAYASHI Kenta, HOSAKA Ryosuke

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04 - 2018/03

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    Beta oscillation is generally known as neural oscillation related to movements with a frequency range between 12 and 30 Hz. It, however, remains unclear what type of information is carried in beta oscillation. Optogenetics is capable of manipulating neural activity by targeting specific cells at millisecond precision. The proposed research will investigate if modulation of beta oscillations induced optogenetically can cause any changes in arm movement of macaque monkeys, thereby elucidating causality between beta oscillation and motor output. Our preliminary results showed a high level of expression of light-gated ion channel (ChR2) in the primary motor cortex in monkeys, and arm-movements were induced by optical stimuli. Thus, our results will be a basis to use optogenetic approaches to macaque monkeys in order to enhance our understanding of motor cortical circuits and their relation to behaviors.

Teaching Experience 4

  1. 保健学科卒業研究　Tohoku University

  2. 高次医学修練（実験発表指導）　Tohoku University

  3. 基礎医学修練（実験及び論文発表の指導）　東北大学

  4. 生体機能学実習（脳波計測、筋電計測、細胞生理学、薬理学、心電図）　東北大学