Details of the Researcher

PHOTO

Tatsuhiko Hoshino
Section
Advanced Institute for Marine Ecosystem Change
Job title
Professor
Degree

Research History 12

  • 2025/04 - Present
    Hiroshima University

  • 2024/06 - Present
    Tohoku University

  • 2023/04 - Present
    Kochi University

  • 2014 - Present
    海洋研究開発機構　高知コア研究所　主任研究員

  • 2009/11 - Present
    海洋研究開発機構　高知コア研究所　研究員

  • 2016/04 - 2025/04
    Hiroshima University

  • 2008/04 - 2009/10
    The University of Tokyo

  • 2008/04 - 2009/10
    オーフス大学（デンマーク）　生態学部　博士研究員

  • 2008/04 - 2009/10
    日本学術振興会　特別研究員SPD

  • 2006/04 - 2008/03
    ウィーン大学（オーストリア）　微生物生態学科　博士研究員

  • 2006/04 - 2008/03
    日本学術振興会　海外特別研究員

  • 2004/04 - 2006/03
    Waseda University　Faculty of Science and Engineering

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Education 1

  • Waseda University　Graduate School of Science and Engineering

    2002/04 - 2005/03

Committee Memberships 4

  • 日本地球掘削科学コンソーシアム (J-DESC)　科学推進専門部会委員

    2024 - Present

  • IODP3　Science Evaluation Panel

    2025/04 - 2027/03

  • 科学技術予測センター　専門調査員

    2022 - 2025

  • 日本地球掘削科学コンソーシアム (J-DESC)　科学技術専門部会委員

    2015 - 2019

Research Interests 5

  • 海洋掘削科学

  • 進化

  • 遺伝子解析

  • 環境微生物

  • 微生物生態

Research Areas 3

  • Life sciences / Biodiversity and systematics /

  • Environmental science/Agricultural science / Landscape science /

  • Environmental science/Agricultural science / Environmental agriculture /

Awards 3

  1. Deep Life Paper awards of 2020

    2021/03　The International Center for Deep Life Investigation　Global diversity of microbial communities in marine sediment

  2. PNAS Cozzarreli Prize 2017

    2018/12　米国アカデミー

  3. 年会優秀発表賞

    2002/03　日本水環境学会

Papers 56

  1. Temperature limits to deep subseafloor life in the Nankai Trough subduction zone International-journal Peer-reviewed

    Verena B. Heuer, Fumio Inagaki, Yuki Morono, Yusuke Kubo, Arthur J. Spivack, Bernhard Viehweger, Tina Treude, Felix Beulig, Florence Schubotz, Satoshi Tonai, Stephen A. Bowden, Margaret Cramm, Susann Henkel, Takehiro Hirose, Kira Homola, Tatsuhiko Hoshino, Akira Ijiri, Hiroyuki Imachi, Nana Kamiya, Masanori Kaneko, Lorenzo Lagostina, Hayley Manners, Harry-Luke McClelland, Kyle Metcalfe, Natsumi Okutsu, Donald Pan, Maija J. Raudsepp, Justine Sauvage, Man-Yin Tsang, David T. Wang, Emily Whitaker, Yuzuru Yamamoto, Kiho Yang, Lena Maeda, Rishi R. Adhikari, Clemens Glombitza, Yohei Hamada, Jens Kallmeyer, Jenny Wendt, Lars Wörmer, Yasuhiro Yamada, Masataka Kinoshita, Kai-Uwe Hinrichs

    Science　370　(6521)　1230-1234　2020/12/04

    Publisher: American Association for the Advancement of Science (AAAS)

    DOI： 10.1126/science.abd7934 　

    ISSN： 0036-8075

    eISSN： 1095-9203

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    Microorganisms in marine subsurface sediments substantially contribute to global biomass. Sediments warmer than 40°C account for roughly half the marine sediment volume, but the processes mediated by microbial populations in these hard-to-access environments are poorly understood. We investigated microbial life in up to 1.2-kilometer-deep and up to 120°C hot sediments in the Nankai Trough subduction zone. Above 45°C, concentrations of vegetative cells drop two orders of magnitude and endospores become more than 6000 times more abundant than vegetative cells. Methane is biologically produced and oxidized until sediments reach 80° to 85°C. In 100° to 120°C sediments, isotopic evidence and increased cell concentrations demonstrate the activity of acetate-degrading hyperthermophiles. Above 45°C, populated zones alternate with zones up to 192 meters thick where microbes were undetectable.

  2. Global diversity of microbial communities in marine sediment Peer-reviewed

    Tatsuhiko Hoshino, Hideyuki Doi, Go-Ichiro Uramoto, Lars Wörmer, Rishi R. Adhikari, Nan Xiao, Yuki Morono, Steven D’Hondt, Kai-Uwe Hinrichs, Fumio Inagaki

    Proceedings of the National Academy of Sciences　117　(44)　27587-27597　2020/11/03

    DOI： 10.1073/pnas.1919139117 　

    ISSN： 0027-8424

    eISSN： 1091-6490

  3. Aerobic microbial life persists in oxic marine sediment as old as 101.5 million years. International-journal Peer-reviewed

    Yuki Morono, Motoo Ito, Tatsuhiko Hoshino, Takeshi Terada, Tomoyuki Hori, Minoru Ikehara, Steven D'Hondt, Fumio Inagaki

    Nature communications　11　(1)　3626-3626　2020/07/28

    DOI： 10.1038/s41467-020-17330-1 　

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    Sparse microbial populations persist from seafloor to basement in the slowly accumulating oxic sediment of the oligotrophic South Pacific Gyre (SPG). The physiological status of these communities, including their substrate metabolism, is previously unconstrained. Here we show that diverse aerobic members of communities in SPG sediments (4.3‒101.5 Ma) are capable of readily incorporating carbon and nitrogen substrates and dividing. Most of the 6986 individual cells analyzed with nanometer-scale secondary ion mass spectrometry (NanoSIMS) actively incorporated isotope-labeled substrates. Many cells responded rapidly to incubation conditions, increasing total numbers by 4 orders of magnitude and taking up labeled carbon and nitrogen within 68 days after incubation. The response was generally faster (on average, 3.09 times) for nitrogen incorporation than for carbon incorporation. In contrast, anaerobic microbes were only minimally revived from this oxic sediment. Our results suggest that microbial communities widely distributed in organic-poor abyssal sediment consist mainly of aerobes that retain their metabolic potential under extremely low-energy conditions for up to 101.5 Ma.

  4. Microbial dormancy in the marine subsurface: Global endospore abundance and response to burial Peer-reviewed

    Woermer Lars, Hoshino Tatsuhiko, Bowles Marshall W, Viehweger Bernhard, Adhikari Rishi R, Xiao Nan, Uramoto Go-ichiro, Koenneke Martin, Lazar Cassandre S, Morono Yuki, Inagaki Fumio, Hinrichs Kai-Uwe

    SCIENCE ADVANCES　5　(2)　2019/02

    DOI： 10.1126/sciadv.aav1024 　

    ISSN： 2375-2548

  5. Abundance and distribution of Archaea in the subseafloor sedimentary biosphere Peer-reviewed

    Tatsuhiko Hoshino, Fumio Inagaki

    ISME Journal　2018/01

    DOI： 10.1038/s41396-018-0253-3 　

    ISSN： 1751-7362

  6. Impact of Anaerobic Cold Storage on Subseafloor Microbial Communities

    Tastuhiko Hoshino, Kana Jitsuno, Yohei Nishikawa, Ken Ikehara, Michael Strasser, Haruko Takeyama, Fumio Inagaki

    Journal of Geophysical Research: Biogeosciences　130　(12)　2025/12/23

    Publisher: American Geophysical Union (AGU)

    DOI： 10.1029/2025jg009286 　

    ISSN： 2169-8953

    eISSN： 2169-8961

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    Abstract Sediment cores recovered from the deep sea often cannot be sampled for microbiological analysis immediately due to the need for core splitting and processing for subsequent onboard measurements and core storage. Consequently, sections are often stored at 4°C under anaerobic conditions for extended periods. However, the impact of the temporal storage on microbiological analysis remains uncertain. In this study, using paired sediment samples from the Japan Trench collected during International Ocean Discovery Program (IODP) Expedition 386, we performed comparative analysis of microbial community composition between samples immediately frozen after core recovery onboard and samples stored anaerobically for 9–11 months onshore. Although the overall community structure was broadly preserved, we observed substantial increases in some opportunistic taxa (e.g., Shewanella, Psychrobacter, and Firmicutes) and a marked decrease in archaeal abundance and diversity. Nevertheless, more than 95% of amplicon sequence variants (ASVs) were retained, and key subseafloor bacterial lineages such as Atribacterota and Chloroflexi showed no significant change in abundance. These results indicate that although long‐term cold storage can introduce identifiable preservation‐related biases, DNA‐based analyses of archived cores can still provide meaningful insights into subseafloor microbial communities when interpreted with appropriate caution.

  7. Spatiotemporal distribution of eukaryotic environmental DNA in global subseafloor sediments. Peer-reviewed

    Hoshino, T, Inagaki, F

    Progress in Earth and Planetary Sciences　11　1-9　2024/04/07

    DOI： 10.1186/s40645-024-00621-2 　

  8. The copy number of the eukaryotic rRNA gene can be counted comprehensively Peer-reviewed

    Akinori Yabuki, Tatsuhiko Hoshino, Tamiko Nakamura, Keiko Mizuno

    MicrobiologyOpen　13　(2)　2024/03/04

    Publisher: Wiley

    DOI： 10.1002/mbo3.1399 　

    ISSN： 2045-8827

    eISSN： 2045-8827

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    Abstract Gene sequence has been widely used in molecular ecology. For instance, the ribosomal RNA (rRNA) gene has been widely used as a biological marker to understand microbial communities. The variety of the detected rRNA gene sequences reflects the diversity of the microorganisms existing in the analyzed sample. Their biomass can also be estimated by applying quantitative sequencing with information on rRNA gene copy numbers in genomes; however, information on rRNA gene copy numbers is still limited. Especially, the copy number in microbial eukaryotes is much less understood than that of prokaryotes, possibly because of the large and complex structure of eukaryotic genomes. In this study, we report an alternative approach that is more appropriate than the existing method of quantitative sequencing and demonstrate that the copy number of eukaryotic rRNA can be measured efficiently and comprehensively. By applying this approach widely, information on the eukaryotic rRNA copy number can be determined, and their community structures can be depicted and compared more efficiently.

  9. Comparative single-cell genomics of Atribacterota JS1 in the Japan Trench hadal sedimentary biosphere Peer-reviewed

    Kana Jitsuno, Tatsuhiko Hoshino, Yohei Nishikawa, Masato Kogawa, Katsuhiko Mineta, Michael Strasser, Ken Ikehara, Jeremy Everest, Lena Maeda, Fumio Inagaki, Haruko Takeyama, Piero Bellanova, Morgane Brunet, Zhirong Cai, Antonio Cattaneo, Katharina Hochmuth, Kanhsi Hsiung, Takashi Ishizawa, Takuya Itaki, Kana Jitsuno, Joel Johnson, Toshiya Kanamatsu, Myra Keep, Arata Kioka, Christian Maerz, Cecilia McHugh, Aaron Micallef, Luo Min, Dhananjai Pandey, Jean Noel Proust, Troy Rasbury, Natascha Riedinger, Rui Bao, Yasufumi Satoguchi, Derek Sawyer, Chloe Seibert, Maxwell Silver, Susanne Straub, Joonas Virtasalo, Yonghong Wang, Ting-Wei Wu, Sarah Zellers, Martin Kölling, Jyh-Jaan Steven Huang, Yoshitaka Nagahashi

    mSphere　2024/01/03

    Publisher: American Society for Microbiology

    DOI： 10.1128/msphere.00337-23 　

    eISSN： 2379-5042

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    ABSTRACT Deep-sea and subseafloor sedimentary environments host heterotrophic microbial communities that contribute to Earth’s carbon cycling. However, the potential metabolic functions of individual microorganisms and their biogeographical distributions in hadal ocean sediments remain largely unexplored. In this study, we conducted single-cell genome sequencing on sediment samples collected from six sites (7,445–8,023 m water depth) along an approximately 500 km transect of the Japan Trench during the International Ocean Discovery Program Expedition 386. A total of 1,886 single-cell amplified genomes (SAGs) were obtained, offering comprehensive genetic insights into sedimentary microbial communities in surface sediments (<1 m depth) above the sulfate-methane transition zone along the Japan Trench. Our genome data set included 269 SAGs from Atribacterota JS1, the predominant bacterial clade in these hadal environments. Phylogenetic analysis classified SAGs into nine distinct phylotypes, whereas metagenome-assembled genomes were categorized into only two phylotypes, advancing JS1 diversity coverage through a single cell-based approach. Comparative genomic analysis of JS1 lineages from different habitats revealed frequent detection of genes related to organic carbon utilization, such as extracellular enzymes like clostripain and α-amylase, and ABC transporters of oligopeptide from Japan Trench members. Furthermore, specific JS1 phylotypes exhibited a strong correlation with in situ methane concentrations and contained genes involved in glycine betaine metabolism. These findings suggest that the phylogenomically diverse and novel Atribacterota JS1 is widely distributed in Japan Trench sediment, playing crucial roles in carbon cycling within the hadal sedimentary biosphere. IMPORTANCE The Japan Trench represents tectonically active hadal environments associated with Pacific plate subduction beneath the northeastern Japan arc. This study, for the first time, documented a large-scale single-cell and metagenomic survey along an approximately 500 km transect of the Japan Trench, obtaining high-quality genomic information on hadal sedimentary microbial communities. Single-cell genomics revealed the predominance of diverse JS1 lineages not recoverable through conventional metagenomic binning. Their metabolic potential includes genes related to the degradation of organic matter, which contributes to methanogenesis in the deeper layers. Our findings enhance understanding of sedimentary microbial communities at water depths exceeding 7,000 m and provide new insights into the ecological role of biogeochemical carbon cycling in the hadal sedimentary biosphere.

  10. Origins of sediments and fluids in submarine mud volcanoes off Tanegashima Island, northern Ryukyu Trench, Japan Peer-reviewed

    Ijiri, A, Setoguchi, R, Mitsutome, Y, Toki, T, Murayama, M, Hagino, K, Hamada, Y, Yamagata, T, Matsuzaki, H, Tanikawa, W, Tadai, O, Kitada, K, Hoshino, T, Noguchi, T, Ashi, J, Inagaki, F

    Frontiers in Earth Science　11　2023/06

    Publisher: Frontiers Media SA

    DOI： 10.3389/feart.2023.1206810 　

    eISSN： 2296-6463

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    Mud volcanoes are topographic features through which over-pressurized sediments are intruded upward and erupted, transporting fluids and sediments from the deep subsurface to the surface/seafloor. To understand material and biogeochemical cycling through mud volcanoes, we investigated the origins of sediments and fluids erupted by four submarine mud volcanoes off Tanegashima Island, along the northern Ryukyu Trench: MV#1–3 and MV#14. We estimated the ages of the source sequences of the mud volcano sediments to be middle to late Miocene based on nannofossils and 10Be ages. The sediments were characteristically enriched in illite. The similar mineral compositions and vitrinite reflectance values (0.42%–0.45%) of sediments erupted by the mud volcanoes strongly suggest that each is rooted in the same source sequence. Pore waters had Cl− concentrations ∼40% that of seawater and were proportionally enriched in 18O and depleted in D, indicating the addition of freshwater from the dehydration of clay minerals. However, the smectite and illite contents (<40%) in the clay size fraction ruled out in situ smectite dewatering as the cause of the pore water Cl− dilution. Thus, fluids derived from clay dewatering must have originated from deeper than the source sequence of the mud volcano sediments. Vertical Cl− profiles indicate that the upward fluid advection rate and eruption frequency decrease from MV#3 to MV#2, MV#1, and MV#14 (MV#14 being dormant). At the active mud volcanoes, the C1/C2 ratios (<100) and methane δ13C values (−56‰ to −42‰) indicate that hydrocarbon gases are mostly derived from the thermal decomposition of organic matter in deep sediments where the in situ temperature exceeds 80°C. At the dormant MV#14, high C1/C2 ratios (700–4,000) and low methane δ13C values (ca. −75‰) suggest the limited supply of thermogenic methane and the subsequent shallow methanogenesis. Because vitrinite reflectance values indicate that the source sediments are too immature to produce thermogenic hydrocarbons, the hydrocarbon gases, like the fluids derived from clay dewatering, were probably supplied from deeper than the source strata. The supply of deep fluids into the source sequence might be a universal phenomenon in subduction zones, and may play an important role in mud volcanism and associated biogeochemical cycling.

  11. Genome characterization of two novel deep-sea sediment fungi, Penicillium pacificagyrus sp. nov. and Penicillium pacificasedimenti sp. nov., from South Pacific Gyre subseafloor sediments, highlights survivability. International-journal Peer-reviewed

    Morgan S Sobol, Tatsuhiko Hoshino, Victor Delgado, Taiki Futagami, Chihiro Kadooka, Fumio Inagaki, Brandi Kiel Reese

    BMC genomics　24　(1)　249-249　2023/05/10

    DOI： 10.1186/s12864-023-09320-6 　

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    BACKGROUND: Marine deep subsurface sediments were once thought to be devoid of eukaryotic life, but advances in molecular technology have unlocked the presence and activity of well-known closely related terrestrial and marine fungi. Commonly detected fungi in deep marine sediment environments includes Penicillium, Aspergillus, Cladosporium, Fusarium, and Schizophyllum, which could have important implications in carbon and nitrogen cycling in this isolated environment. In order to determine the diversity and unknown metabolic capabilities of fungi in deep-sea sediments, their genomes need to be fully analyzed. In this study, two Penicillium species were isolated from South Pacific Gyre sediment enrichments during Integrated Ocean Drilling Program Expedition 329. The inner gyre has very limited productivity, organic carbon, and nutrients. RESULTS: Here, we present high-quality genomes of two proposed novel Penicillium species using Illumina HiSeq and PacBio sequencing technologies. Single-copy homologues within the genomes were compared to other closely related genomes using OrthoMCL and maximum-likelihood estimation, which showed that these genomes were novel species within the genus Penicillium. We propose to name isolate SPG-F1 as Penicillium pacificasedimenti sp. nov. and SPG-F15 as Penicillium pacificagyrus sp. nov. The resulting genome sizes were 32.6 Mbp and 36.4 Mbp, respectively, and both genomes were greater than 98% complete as determined by the presence of complete single-copy orthologs. The transposable elements for each genome were 4.87% for P. pacificasedimenti and 10.68% for P. pacificagyrus. A total of 12,271 genes were predicted in the P. pacificasedimenti genome and 12,568 genes in P. pacificagyrus. Both isolates contained genes known to be involved in the degradation of recalcitrant carbon, amino acids, and lignin-derived carbon. CONCLUSIONS: Our results provide the first constructed genomes of novel Penicillium isolates from deep marine sediments, which will be useful for future studies of marine subsurface fungal diversity and function. Furthermore, these genomes shed light on the potential impact fungi in marine sediments and the subseafloor could have on global carbon and nitrogen biogeochemical cycles and how they may be persisting in the most energy-limited sedimentary biosphere.

  12. Terpene Synthase Gene Amplicons from Subseafloor Sediment. International-journal Peer-reviewed

    Ruth L Schmidt, Tatsuhiko Hoshino, Yuki Morono, Julien Tremblay, Dana Ulanova

    Microbiology resource announcements　e0103722　2023/01/18

    DOI： 10.1128/mra.01037-22 　

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    In this announcement, we present the set of putative terpene synthase (TS) gene fragments detected in a subseafloor sediment sample collected off Shimokita Peninsula, Japan. This data set contains sequences with 72 to 100% identity to TS from actinobacteria and cyanobacteria.

  13. Simultaneous absolute quantification and sequencing of fish environmental DNA in a mesocosm by quantitative sequencing technique

    Tatsuhiko Hoshino, Ryohei Nakao, Hideyuki Doi, Toshifumi Minamoto

    Scientific Reports　11　(1)　2021/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-021-83318-6 　

    eISSN： 2045-2322

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    <title>Abstract</title>The combination of high-throughput sequencing technology and environmental DNA (eDNA) analysis has the potential to be a powerful tool for comprehensive, non-invasive monitoring of species in the environment. To understand the correlation between the abundance of eDNA and that of species in natural environments, we have to obtain quantitative eDNA data, usually via individual assays for each species. The recently developed quantitative sequencing (qSeq) technique enables simultaneous phylogenetic identification and quantification of individual species by counting random tags added to the 5′ end of the target sequence during the first DNA synthesis. Here, we applied qSeq to eDNA analysis to test its effectiveness in biodiversity monitoring. eDNA was extracted from water samples taken over 4 days from aquaria containing five fish species (<italic>Hemigrammocypris neglectus</italic>, <italic>Candidia temminckii</italic>, <italic>Oryzias latipes</italic>, <italic>Rhinogobius flumineus</italic>, and <italic>Misgurnus anguillicaudatus</italic>), and quantified by qSeq and microfluidic digital PCR (dPCR) using a TaqMan probe. The eDNA abundance quantified by qSeq was consistent with that quantified by dPCR for each fish species at each sampling time. The correlation coefficients between qSeq and dPCR were 0.643, 0.859, and 0.786 for <italic>H. neglectus</italic>, <italic>O. latipes</italic>, and <italic>M. anguillicaudatus</italic>, respectively, indicating that qSeq accurately quantifies fish eDNA.

  14. Hydrothermal formation of iron-oxyhydroxide chimney mounds in a shallow semi-enclosed bay at Satsuma Iwo-Jima Island, Kagoshima, Japan Peer-reviewed

    Shoichi Kiyokawa, Takashi Kuratomi, Tatsuhiko Hoshino, Shusaku Goto, Minoru Ikehara

    Bulletin of the Geological Society of America　133　(9-10)　1890-1908　2021

    DOI： 10.1130/B35782.1 　

    ISSN： 0016-7606

    eISSN： 1943-2674

  15. A Seafloor Survey Using Multiple Methodologies to Verify the Existence of a Village Submerged after a Historical Nankai Earthquake in Nomi Bay, Kochi Prefecture

    TANIKAWA Wataru, OKAZAKI Keishi, TOKUYAMA Hidekazu, MURAYAMA Masafumi, IJIRI Akira, HIROSE Takehiro, URAMOTO Go-ichiro, HOSHINO Tatsuhiko, TANAKA Kouki, YAMAMOTO Yuhji, HAMADA Yohei

    Bulletin on Coastal Oceanography　59　(1)　2021

    Publisher: Coastal Oceanography Research Committee, the Oceanographic Society of Japan

    DOI： 10.32142/engankaiyo.2021.4.001 　

    ISSN： 1342-2758

  16. Innovative microfossil (radiolarian) analysis using a system for automated image collection and AI-based classification of species Peer-reviewed

    Takuya Itaki, Yosuke Taira, Naoki Kuwamori, Hitoshi Saito, Minoru Ikehara, Tatsuhiko Hoshino

    Scientific Reports　10　(1)　2020/12

    DOI： 10.1038/s41598-020-77812-6 　

    eISSN： 2045-2322

  17. Deep microbial proliferation at the basalt interface in 33.5–104 million-year-old oceanic crust Peer-reviewed

    Yohey Suzuki, Seiya Yamashita, Mariko Kouduka, Yutaro Ao, Hiroki Mukai, Satoshi Mitsunobu, Hiroyuki Kagi, Steven D’Hondt, Fumio Inagaki, Yuki Morono, Tatsuhiko Hoshino, Naotaka Tomioka, Motoo Ito

    Communications Biology　3　(1)　2020/12/01

    DOI： 10.1038/s42003-020-0860-1 　

    eISSN： 2399-3642

  18. A New Method for Quality Control of Geological Cores by X-Ray Computed Tomography: Application in IODP Expedition 370 Peer-reviewed

    Satoshi Tonai, Yusuke Kubo, Man-Yin Tsang, Stephen Bowden, Kotaro Ide, Takehiro Hirose, Nana Kamiya, Yuzuru Yamamoto, Kiho Yang, Yasuhiro Yamada, Yuki Morono, Verena B. Heuer, Fumio Inagaki, Lena Maeda, Margaret Cramm, Susann Henkel, Kira Homola, Tatsuhiko Hoshino, Akira Ijiri, Hiroyuki Imachi, Masanori Kaneko, Lorenzo Lagostina, Hayley Manners, Ilarry-Luke McClelland, Kyle Metcalfe, Natsumi Okutsu, Donald Pan, Maija Jocelyn Raudsepp, Justine Sauvage, Florence Schubotz, Arthur Spivack, Tina Treude, Bernhard Viehweger, David T. Wang, Emily Whitaker, Masataka Kinoshita

    FRONTIERS IN EARTH SCIENCE　7　2019/05

    DOI： 10.3389/feart.2019.00117 　

    eISSN： 2296-6463

  19. Naturally occurring, microbial induced smectite-to-illite reaction Peer-reviewed

    Jinwook Kim, Hailiang Dong, Kiho Yang, Hanbeom Park, W Crawford Elliott, Arthur Spivack, Tae-hee Koo, Gilyoung Kim, Yuki Morono, Susann Henkel, Fumio Inagaki, Qiang Zeng, Tatsuhiko Hoshino, Verena B Heuer

    Geology　47　(6)　535-539　2019/04

  20. Cultivable microbial community in 2-km-deep, 20-million-year-old subseafloor coalbeds through ~1000 days anaerobic bioreactor cultivation. International-journal Peer-reviewed

    Hiroyuki Imachi, Eiji Tasumi, Yoshihiro Takaki, Tatsuhiko Hoshino, Florence Schubotz, Shuchai Gan, Tzu-Hsuan Tu, Yumi Saito, Yuko Yamanaka, Akira Ijiri, Yohei Matsui, Masayuki Miyazaki, Yuki Morono, Ken Takai, Kai-Uwe Hinrichs, Fumio Inagaki

    Scientific reports　9　(1)　2305-2305　2019/02/19

    DOI： 10.1038/s41598-019-38754-w 　

    ISSN： 2045-2322

  21. Draft Genome Sequences of Penicillium spp. from Deeply Buried Oligotrophic Marine Sediments. International-journal Peer-reviewed

    Morgan S Sobol, Tatsuhiko Hoshino, Taiki Futagami, Fumio Inagaki, Brandi Kiel Reese

    Microbiology resource announcements　8　(6)　2019/02

    DOI： 10.1128/MRA.01613-18 　

    ISSN： 2576-098X

  22. Microbial Metabolism and Community Dynamics in Hydraulic Fracturing Fluids Recovered From Deep Hydrocarbon-Rich Shale. International-journal Peer-reviewed

    Yuki Morono, Jessie R Wishart, Motoo Ito, Akira Ijiri, Tatsuhiko Hoshino, Marta Torres, Circe Verba, Takeshi Terada, Fumio Inagaki, Frederick S Colwell

    Frontiers in microbiology　10　376-376　2019

    DOI： 10.3389/fmicb.2019.00376 　

    ISSN： 1664-302X

  23. In-situ mechanical weakness of subducting sediments beneath a plate boundary décollement in the Nankai Trough Peer-reviewed

    Yohei Hamada, Takehiro Hirose, Akira Ijiri, Yasuhiro Yamada, Yoshinori Sanada, Saneatsu Saito, Noriaki Sakurai, Takamitsu Sugihara, Takahiro Yokoyama, Tomokazu Saruhashi, Tatsuhiko Hoshino, Nana Kamiya, Stephen Bowden, Margaret Cramm, Susann Henkel, Kira Homola, Hiroyuki Imachi, Masanori Kaneko, Lorenzo Lagostina, Hayley Manners, Harry-Luke McClelland, Kyle Metcalfe, Natsumi Okutsu, Donald Pan, Maija Jocelyn Raudsepp, Justine Sauvage, Florence Schubotz, Arthur Spivack, Satoshi Tonai, Tina Treude, Man-Yin Tsang, Bernhard Viehweger, David T. Wang, Emily Whitaker, Yuzuru Yamamoto, Kiho Yang, Masataka Kinoshita, Lena Maeda, Yusuke Kubo, Yuki Morono, Fumio Inagaki, Verena B. Heuer

    Progress in Earth and Planetary Science　5　(1)　2018/12

    DOI： 10.1186/s40645-018-0228-z 　

    ISSN： 2197-4284

    eISSN： 2197-4284

  24. Magnetic Mineral Diagenesis in a High Temperature and Deep Methanic Zone in Izu Rear Arc Marine Sediments, Northwest Pacific Ocean Peer-reviewed

    Kars Myriam, Musgrave Robert J, Hoshino Tatsuhiko, Jonas Ann-Sophie, Bauersachs Thorsten, Inagaki Fumio, Kodama Kazuto

    JOURNAL OF GEOPHYSICAL RESEARCH-SOLID EARTH　123　(10)　8331-8348　2018/10

    DOI： 10.1029/2018JB015861 　

    ISSN： 2169-9313

  25. Magnetic Mineral Diagenesis in a High Temperature and Deep Methanic Zone in Izu Rear Arc Marine Sediments, Northwest Pacific Ocean Peer-reviewed

    JGR Solid Earth　2018/09

    DOI： 10.1029/2018JB015861 　

  26. Collaborative environmental DNA sampling from petal surfaces of flowering cherry Cerasus × yedoensis 'Somei-yoshino' across the Japanese archipelago. Peer-reviewed

    Tazro Ohta, Takeshi Kawashima, Natsuko O Shinozaki, Akito Dobashi, Satoshi Hiraoka, Tatsuhiko Hoshino, Keiichi Kanno, Takafumi Kataoka, Shuichi Kawashima, Motomu Matsui, Wataru Nemoto, Suguru Nishijima, Natsuki Suganuma, Haruo Suzuki, Y-H Taguchi, Yoichi Takenaka, Yosuke Tanigawa, Momoka Tsuneyoshi, Kazutoshi Yoshitake, Yukuto Sato, Riu Yamashita, Kazuharu Arakawa, Wataru Iwasaki

    Journal of plant research　131　(4)　709-717　2018/07

    DOI： 10.1007/s10265-018-1017-x 　

    ISSN： 0918-9440

  27. Deep-biosphere methane production stimulated by geofluids in the Nankai accretionary complex. International-journal Peer-reviewed

    Akira Ijiri, Fumio Inagaki, Yusuke Kubo, Rishi R Adhikari, Shohei Hattori, Tatsuhiko Hoshino, Hiroyuki Imachi, Shinsuke Kawagucci, Yuki Morono, Yoko Ohtomo, Shuhei Ono, Sanae Sakai, Ken Takai, Tomohiro Toki, David T Wang, Marcos Y Yoshinaga, Gail L Arnold, Juichiro Ashi, David H Case, Tomas Feseker, Kai-Uwe Hinrichs, Yojiro Ikegawa, Minoru Ikehara, Jens Kallmeyer, Hidenori Kumagai, Mark A Lever, Sumito Morita, Ko-Ichi Nakamura, Yuki Nakamura, Manabu Nishizawa, Victoria J Orphan, Hans Røy, Frauke Schmidt, Atsushi Tani, Wataru Tanikawa, Takeshi Terada, Hitoshi Tomaru, Takeshi Tsuji, Urumu Tsunogai, Yasuhiko T Yamaguchi, Naohiro Yoshida

    Science advances　4　(6)　eaao4631　2018/06

    DOI： 10.1126/sciadv.aao4631 　

  28. Exploration of the deep-subseafloor-biosphere frontiers: Achievements and perspectives

    稲垣史生, 稲垣史生, 稲垣史生, 諸野祐樹, 諸野祐樹, 星野辰彦, 星野辰彦, 井尻暁, 井尻暁, XIAO Nan, 鈴木志野, 石井俊一, 浦本豪一郎, 浦本豪一郎, 寺田武志, 井町寛之, 井町寛之, 久保雄介

    地質学雑誌　124　(1)　77-92　2018

    Publisher: The Geological Society of Japan

    DOI： 10.5575/geosoc.2017.0079 　

    ISSN： 0016-7630

    More details Close

    <p>Scientific ocean drilling over the past half-century has significantly expanded our knowledge of life, ocean, and Earth. The discovery of the spatially vast deep-subseafloor biosphere, a milestone scientific achievement, has extended the planetary habitability of life on Earth. To date, multiple lines of evidence form the core samples have demonstrated that a remarkable amount of physiologically unknown microbial life is present deep beneath the ocean floor where water supply and energy substrates are severely limited. Although subseafloor microbial ecosystems generally function extremely slowly, the consequence of long-term activity on geological timescales may play significant ecological roles in global biogeochemical carbon and other elemental cycles.</p>

  29. Assessment of capacity to capture DNA aerosols by clean filters for molecular biology experiments Peer-reviewed

    Yuki Morono, Tatsuhiko Hoshino, Takeshi Terada, Taketo Suzuki, Takahiro Sato, Hisashi Yuasa, Yuji Kubota, Fumio Inagaki

    Microbes and Environments　33　(2)　222-226　2018/01

    DOI： 10.1264/jsme2.ME18012 　

    ISSN： 1342-6311

  30. Unusual metabolic diversity of hyperalkaliphilic microbial communities associated with subterranean serpentinization at The Cedars Peer-reviewed

    Shino Suzuki, Shun'ichi Ishii, Tatsuhiko Hoshino, Amanda Rietze, Aaron Tenney, Penny L. Morrill, Fumio Inagaki, J. Gijs Kuenen, Kenneth H. Nealson

    ISME JOURNAL　11　(11)　2584-2598　2017/11

    DOI： 10.1038/ismej.2017.111 　

    ISSN： 1751-7362

    eISSN： 1751-7370

  31. Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds Peer-reviewed

    Elizabeth Trembath-Reichert, Yuki Morono, Akira Ijiri, Tatsuhiko Hoshino, Katherine S. Dawson, Fumio lnagaki, Victoria J. Orphan

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　114　(44)　E9206-E9215　2017/10

    DOI： 10.1073/pnas.1707525114 　

    ISSN： 0027-8424

  32. Estimation of the influence of sequencing errors and distribution of random sequence tags on quantitative sequencing Peer-reviewed

    Tatsuhiko Hoshino, Yohei Hamada

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING　124　(3)　359-364　2017/09

    DOI： 10.1016/j.jbiosc.2017.04.003 　

    ISSN： 1389-1723

    eISSN： 1347-4421

  33. Atribacteria from the Subseafloor Sedimentary Biosphere Disperse to the Hydrosphere through Submarine Mud Volcanoes

    Tatsuhiko Hoshino, Tomohiro Toki, Akira Ijiri, Yuki Morono, Hideaki Machiyama, Juichiro Ashi, Kei Okamura, Fumio Inagaki

    FRONTIERS IN MICROBIOLOGY　8　2017/06

    DOI： 10.3389/fmicb.2017.01135 　

    ISSN： 1664-302X

  34. Distribution of anaerobic carbon monoxide dehydrogenase genes in deep subseafloor sediments Peer-reviewed

    T. Hoshino, F. Inagaki

    LETTERS IN APPLIED MICROBIOLOGY　64　(5)　355-363　2017/05

    DOI： 10.1111/lam.12727 　

    ISSN： 0266-8254

    eISSN： 1472-765X

  35. Application of Stochastic Labeling with Random-Sequence Barcodes for Simultaneous Quantification and Sequencing of Environmental 16S rRNA Genes Peer-reviewed

    Tatsuhiko Hoshino, Fumio Inagaki

    PLOS ONE　12　(1)　2017/01

    DOI： 10.1371/journal.pone.0169431 　

    ISSN： 1932-6203

  36. Dense microbial community on a ferromanganese nodule from the ultra-oligotrophic South Pacific Gyre: Implications for biogeochemical cycles Peer-reviewed

    Fumito Shiraishi, Satoshi Mitsunobu, Katsuhiko Suzuki, Tatsuhiko Hoshino, Yuki Morono, Fumio Inagaki

    EARTH AND PLANETARY SCIENCE LETTERS　447　10-20　2016/08

    DOI： 10.1016/j.epsl.2016.04.021 　

    ISSN： 0012-821X

    eISSN： 1385-013X

  37. IODP Expedition 337: Deep Coalbed Biosphere off Shimokita - Microbial processes and hydrocarbon system associated with deeply buried coalbed in the ocean Peer-reviewed

    Fumio Inagaki, Kai Uwe Hinrichs, Yusuke Kubo, Monika Bihan, Stephen A. Bowden, Marshall Bowles, Marcus Elvert, Clemens Glombitza, Doris Gross, Guy J. Harrington, Verena Heuer, Wei Li Hong, Tomoyuki Hori, Tatsuhiko Hoshino, Akira Ijiri, Hiroyuki Imachi, Motoo Ito, Masanori Kaneko, Mark A. Lever, Kevin Li, David Limmer, Yu Shih Lin, Chang Hong Liu, Barbara A. Methé, Sumito Morita, Yuki Morono, Masafumi Murayama, Naohiko Ohkouchi, Shuhei Ono, Young Soo Park, Stephen C. Phillips, Xavier Prieto-Mollar, Marcella Purkey, Natascha Riedinger, Yoshinori Sanada, Justine Sauvage, Glen Snyder, Rita Susilawati, Yoshinori Takano, Wataru Tanikawa, Eiji Tasumi, Takeshi Terada, Hitoshi Tomaru, Elizabeth Trembath-Reichert, David T. Wang, Yasuhiro Yamada

    Scientific Drilling　21　17-28　2016/06/27

    DOI： 10.5194/sd-21-17-2016 　

    ISSN： 1816-8957

    eISSN： 1816-3459

  38. Ecophysiology of Zetaproteobacteria Associated with Shallow Hydrothermal Iron-Oxyhydroxide Deposits in Nagahama Bay of Satsuma Iwo-Jima, Japan

    Tatsuhiko Hoshino, Takashi Kuratomi, Yuki Morono, Tomoyuki Hori, Hisashi Oiwane, Shoichi Kiyokawa, Fumio Inagaki

    FRONTIERS IN MICROBIOLOGY　6　2016/01

    DOI： 10.3389/fmicb.2015.01554 　

    ISSN： 1664-302X

  39. Exploring deep microbial life in coal-bearing sediment down to ∼2.5 km below the ocean floor Peer-reviewed

    F. Inagaki, K. U. Hinrichs, Y. Kubo, M. W. Bowles, V. B. Heuer, W. L. Hong, T. Hoshino, A. Ijiri, H. Imachi, M. Ito, M. Kaneko, M. A. Lever, Y. S. Lin, B. A. Methé, S. Morita, Y. Morono, W. Tanikawa, M. Bihan, S. A. Bowden, M. Elvert, C. Glombitza, D. Gross, G. J. Harrington, T. Hori, K. Li, D. Limmer, C. H. Liu, M. Murayama, N. Ohkouchi, S. Ono, Y. S. Park, S. C. Phillips, X. Prieto-Mollar, M. Purkey, N. Riedinger, Y. Sanada, J. Sauvage, G. Snyder, R. Susilawati, Y. Takano, E. Tasumi, T. Terada, H. Tomaru, E. Trembath-Reichert, D. T. Wang, Y. Yamada

    Science　349　(6246)　420-424　2015/07/24

    DOI： 10.1126/science.aaa6882 　

    ISSN： 0036-8075

    eISSN： 1095-9203

  40. Hot-Alkaline DNA Extraction Method for Deep-Subseafloor Archaeal Communities (vol 80, pg 1985, 2014) Peer-reviewed

    Yuki Morono, Takeshi Terada, Tatsuhiko Hoshino, Fumio Inagaki

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY　80　(15)　4785-4785　2014/08

    DOI： 10.1128/AEM.01499-14 　

    ISSN： 0099-2240

    eISSN： 1098-5336

  41. Biological CO2 conversion to acetate in subsurface coal-sand formation using a high-pressure reactor system Peer-reviewed

    Yoko Ohtomo, Akira Ijiri, Yojiro Ikegawa, Masazumi Tsutsumi, Hiroyuki Imachi, Go-Ichiro Uramoto, Tatsuhiko Hoshino, Yuki Morono, Sanae Sakai, Yumi Saito, Wataru Tanikawa, Takehiro Hirose, Fumio Inagaki

    FRONTIERS IN MICROBIOLOGY　4　2013/12

    DOI： 10.3389/fmicb.2013.00361 　

    ISSN： 1664-302X

  42. A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription-FOR Peer-reviewed

    Tatsuhiko Hoshino, Fumio Inagaki

    FRONTIERS IN MICROBIOLOGY　4　2013/06

    DOI： 10.3389/fmicb.2013.00160 　

    ISSN： 1664-302X

  43. Metabolically active microbial communities in marine sediment under high-CO2 and low-pH extremes Peer-reviewed

    Katsunori Yanagawa, Yuki Morono, Dirk de Beer, Matthias Haeckel, Michinari Sunamura, Taiki Futagami, Tatsuhiko Hoshino, Takeshi Terada, Ko-ichi Nakamura, Tetsuro Urabe, Gregor Rehder, Antje Boetius, Fumio Inagaki

    ISME JOURNAL　7　(3)　555-567　2013/03

    DOI： 10.1038/ismej.2012.124 　

    ISSN： 1751-7362

    eISSN： 1751-7370

  44. Molecular quantification of environmental DNA using microfluidics and digital PCR Peer-reviewed

    Tatsuhiko Hoshino, Fumio Inagaki

    SYSTEMATIC AND APPLIED MICROBIOLOGY　35　(6)　390-395　2012/09

    DOI： 10.1016/j.syapm.2012.06.006 　

    ISSN： 0723-2020

  45. Comparative study of subseafloor microbial community structures in deeply buried coral fossils and sediment matrices from the Challenger Mound in the Porcupine Seabight Peer-reviewed

    Tatsuhiko Hoshino, Yuki Morono, Takeshi Terada, Hiroyuki Imachi, Timothy G. Ferdelman, Fumio Inagaki

    FRONTIERS IN MICROBIOLOGY　2　2011

    DOI： 10.3389/fmicb.2011.00231 　

    ISSN： 1664-302X

  46. Detection of denitrification genes by in situ rolling circle amplification-fluorescence in situ hybridization to link metabolic potential with identity inside bacterial cells Peer-reviewed

    Tatsuhiko Hoshino, Andreas Schramm

    ENVIRONMENTAL MICROBIOLOGY　12　(9)　2508-2517　2010/09

    DOI： 10.1111/j.1462-2920.2010.02224.x 　

    ISSN： 1462-2912

  47. Quantification of target molecules needed to by fluorescence in situ hybridization (FISH) and catalyzed reporter deposition-FISH Peer-reviewed

    Tatsuhiko Hoshino, L. Safak Yilmaz, Daniel R. Noguera, Holger Daims, Michael Wagner

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY　74　(16)　5068-5077　2008/08

    DOI： 10.1128/AEM.00208-08 　

    ISSN： 0099-2240

  48. RNA microarray for estimating relative abundance of 16S rRNA in microbial communities Peer-reviewed

    Tatsuhiko Hoshino, Kazuhiro Furukawa, Satoshi Tsuneda, Yuhei Inamori

    Journal of Microbiological Methods　69　(2)　406-410　2007/05

    DOI： 10.1016/j.mimet.2007.01.007 　

    ISSN： 0167-7012

  49. Direct profiling of rRNA in saline wastewater treatment samples using an oligonucleotide Microarray Peer-reviewed

    Hidetoshi Urakawa, Junpei Matsumoto, Tatsuhiko Hoshino, Satoshi Tsuneda

    MICROBES AND ENVIRONMENTS　22　(2)　116-122　2007

    ISSN： 1342-6311

  50. Long-term monitoring of the succession of a microbial community in activated sludge from a circulation flush toilet as a closed system Peer-reviewed

    Tatsuhiko Hoshino, Takeshi Terahara, Kenji Yamada, Hideo Okuda, Isao Suzuki, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

    FEMS Microbiology Ecology　55　(3)　459-470　2006/03

    DOI： 10.1111/j.1574-6941.2005.00047.x 　

    ISSN： 0168-6496 1574-6941

  51. Comprehensive Analysis of Cell Wall-Permeabilizing Conditions for Highly Sensitive Fluorescence in Situ Hybridization Peer-reviewed

    Kazuhiro Furukawa, Tatsuhiko Hoshino, Satoshi Tsuneda, Yuhei Inamori

    Microbes and Environments　21　(4)　227-234　2006

    DOI： 10.1264/jsme2.21.227 　

    ISSN： 1347-4405 1342-6311

  52. 硝化グラニュールを利用した高塩濃度産業廃水処理 Peer-reviewed

    星野辰彦, 広沢英信, 松川忠史, 小川知幸, 江尻慶央, 常田聡, 平田彰

    日本水処理生物学会誌　40　(4)　137-142　2004/12

    Publisher: Japanese Society of Water Treatment Biology

    DOI： 10.2521/jswtb.40.137 　

    ISSN： 0910-6758

    More details Close

    Wastewater containing high nitrogen and high salinity is discharged from the processes of recovering precious metals from industrial waste. Because high salinity inhibits metabolic activity of microorganisms, biological nitrogen removal from such a wastewater has a lot of difficulties. The purpose of this study is to develop an effective nitrification denitrification process for industrial saline-wastewater. At first, nitrogen removal from this wastewater was attempted using a normal suspended nitrifying sludge system. In consequence of estimating tolerance of such a sludge system to salinity, nitrification progressed up to 5% of salt concentration. However, keeping MLSS at 5, 000 g/m3 is necessary to achieve practical nitrification rate. Therefore, nitrifying granules resulting from an aerobic upflow fluidized bed reactor for inorganic wastewater containing high concentration of NH4-N were applied to this industrial saline-wastewater. In consequence of operating nitrification reactor at various salt concentrations, MLSS was kept at 12000g/m3 throughout the operation, and sufficient nitrogen removal rate was achieved. Finally, the nitrifying granules were applied to an aerobic reactor in the aerobic-anaerobic nitrogen removal system. As a result, nitrogen in the saline wastewater was completely removed for 150 days. Through these experiments, it is demonstrated that nitrifying granules are effectively applied to nitrogen removal from industrial saline-wastewater.

  53. Monitoring the microbial population dynamics at the start-up stage of wastewater treatment reactor by terminal restriction fragment length polymorphism analysis based on 16s rDNA and rRNA gene sequences Peer-reviewed

    Takeshi Terahara, Tatsuhiko Hoshino, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

    Journal of Bioscience and Bioengineering　98　(6)　425-428　2004

    Publisher: Society of Fermentation and Bioengineering

    DOI： 10.1016/S1389-1723(05)00307-5 　

    ISSN： 1389-1723

  54. In situ PCR for visualizing distribution of a functional gene "amoA" in a biofilm regardless of activity Peer-reviewed

    Tatsuhiko Hoshino, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

    Journal of Biotechnology　105　(1-2)　33-40　2003/10/09

    Publisher: Elsevier

    DOI： 10.1016/S0168-1656(03)00171-8 　

    ISSN： 0168-1656

  55. Characterization of nitrifying granules produced in an aerobic upflow fluidized bed reactor Peer-reviewed

    Satoshi Tsuneda, Tatsuo Nagano, Tatsuhiko Hoshino, Yoshihiro Ejiri, Naohiro Noda, Akira Hirata

    Water Research　37　(20)　4965-4973　2003

    Publisher: Elsevier Ltd

    DOI： 10.1016/j.watres.2003.08.017 　

    ISSN： 0043-1354

  56. Direct Detection by in Situ PCR of the amoA Gene in Biofilm Resulting from a Nitrogen Removal Process Peer-reviewed

    Tatsuhiko Hoshino, Naohiro Noda, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

    Applied and Environmental Microbiology　67　(3-12)　5261-5266　2001

    ISSN： 0099-2240

Show all ︎Show first 5

Misc. 76

  1. Linking sediment porosity to taxon-specific patterns of eDNA preservation in a temperate, semi-enclosed bay

    Tatsuhiko Hoshino, Saki Toyama, Wataru Tanikawa, Masafumi Murayama

    2025/11/19

    Publisher: openRxiv

    DOI： 10.1101/2025.11.19.689188 　

    More details Close

    Abstract Sedimentary environmental DNA provides unique insights into past community dynamics. However, the influence of physical sediment properties, such as porosity, on taxon-specific eDNA preservation remains poorly understood, particularly in temperate inner-bay environments. Therefore, here, we characterized eukaryotic communities in sediment cores from the surface to 6-m depth in the inner and mouth regions of the Uranouchi Bay, a semi-enclosed embayment in temperate Japan. Both 18S rRNA and mitochondrial cytochrome c oxidase subunit I gene metabarcoding revealed marked spatial variation; specifically, inner-bay sediments were enriched in terrestrial plant DNA and dinoflagellates, whereas mouth sediments contained higher proportions of marine diatoms. Vertical profiles further highlighted differences in DNA persistence, with terrestrial and particle-associated taxa retained deeper in the inner-bay sediments. Land–sea connectivity and basin geography strongly shape sedimentary eDNA composition, reflecting both ecological sourcing and preservation bias. We further systematically analyzed the relationship between sediment porosity, a critical physical property governing pore water mobility and solute diffusion, and taxon-specific sedimentary eDNA preservation. To highlight effects of porosity independent of burial depth, we modeled porosity as a function of depth and correlated the residual porosity with the relative abundances of individual phyla. Notably, the phyla exhibited contrasting preservation patterns: Dinoflagellata, Euglenozoa, and Rotifera showed weak to moderate positive correlations with porosity, whereas Chlorophyta and Ochrophyta displayed weak negative correlations. When depth correlations were examined jointly, multiple distinct pattern groups emerged: some taxa (e.g., Dinoflagellata) exhibited negative depth but positive porosity correlations, whereas others showed opposite or neutral patterns. These divergent responses suggest that DNA preservation in marine sediments involves multiple taxon-specific mechanisms rather than a single universal process, with porosity acting as an independent determinant of burial depth. Our results highlight the importance of integrating physical properties of sediments into sedimentary environmental DNA-based paleoenvironmental reconstructions.

  2. Evolutionary stasis and homogeneous selection structure microbial communities in the deep subseafloor sedimentary biosphere

    Tatsuhiko Hoshino, Atsuhiro Sakuma, Rei Kajitani, Hideyuki Doi, Takehiko Ito, Fumio Inagaki

    2025/11/18

    Publisher: openRxiv

    DOI： 10.1101/2025.11.18.689030 　

    More details Close

    The subseafloor biosphere, one of the Earth's largest and most stable microbial habitats, mainly consists of energy-limited sediments where microbial life persists over geological timescales. However, the mechanisms governing microbial community assembly and evolution under condition of extreme energy limitation remains unclear. Here, we analysed a 296 m sediment core off the Shimokita Peninsula using amplicon sequencing, metagenomics, and genome-resolved analyses from 31 depth intervals spanning ~480 kyr. The microbial community composition was governed primarily by homogeneous selection, consistent with persistent environmental uniformity during burial. Genome-resolved analyses of 224 high-quality metagenome-assembled genomes revealed highy conserved gene repertoires and uniformly low ratios of non-synonymous-to-synonymous substitutions, suggesting strong purifying selection with minimal genomic changes over this timescale. Our results indicate that evolutionary stasis is a pervasive feature of microbial life in deep subseafloor sediments, and propose a conceptual framework linking long-term environmental stability to genomic preservation in the Earth's most persistent biosphere.

  3. 日本海溝の超深海堆積物を対象とした細菌および(プロ)ファージのシングルセルゲノム解析

    西川洋平, 西川洋平, 我妻竜太, 我妻竜太, 實野佳奈, 星野辰彦, 稲垣史生, 稲垣史生, 竹山春子, 竹山春子, 竹山春子, 竹山春子

    マリンバイオテクノロジー学会大会講演要旨集　24th　2024

  4. 日本海溝堆積物微生物群集の地理空間的分布

    星野辰彦, 星野辰彦, 西川洋平, 西川洋平, 竹山春子, 竹山春子, 竹山春子, 竹山春子, 稲垣史生, 稲垣史生

    日本微生物生態学会大会(Web)　37th　2024

  5. Emission of dissolved organic carbon from submarine mud volcanoes off Kikaijima Island, Tanagashima Island and Hyuga-nada, Japan

    吉崎結衣, 星野辰彦, 松井洋平, 川口慎介, 竹内誠, 岡村慶, 野口拓郎, 乙坂重嘉, 井尻暁

    日本地球化学会年会要旨集(Web)　71st　2024

  6. 多様性と存在量を同時に推定する環境DNA解析手法の提案

    矢吹彬憲, 星野辰彦, 中村多実子, 水野恵子

    藻類　72　(1)　2024

    ISSN： 0038-1578

  7. Genetic characterization of deep-sea microorganisms in the Japan Trench sediments

    實野佳奈, 實野佳奈, 西川洋平, 西川洋平, 星野辰彦, 稲垣史生, 稲垣史生, 竹山春子, 竹山春子, 竹山春子, 竹山春子

    日本生物工学会大会講演要旨集　75th　2023

  8. 高知県浦の内湾堆積物中の環境DNA解析による生物変遷分析

    星野辰彦, 遠山早紀, 村山雅氏, 神徳理紗

    日本水産学会大会講演要旨集(CD-ROM)　2023　2023

  9. Understanding of past ecosystem using environmental DNA remaining in the sediment of Uranouchi Bay, Kochi Prefecture

    星野辰彦, 谷川亘, 井尻暁, 遠山早紀, 村山雅史, 神徳理紗

    日本水環境学会年会講演集　57th　2023

  10. 日本海溝に生息する超深海微生物群の遺伝的機能の解明

    實野佳奈, 西川洋平, 星野辰彦, 稲垣史生, 稲垣史生, 稲垣史生, 竹山春子, 竹山春子, 竹山春子

    マリンバイオテクノロジー学会大会講演要旨集　23rd　2023

  11. Subseafloor sediment-One of the largest biospheres on Earth-

    星野辰彦

    月刊細胞　55　(14)　2023

    ISSN： 1346-7557

  12. Comprehensive analysis of electroactive microorganisms inhabiting iron-rich chimneys in the bay of Satsuma-Iwo Jima

    石井俊一, 星野辰彦, 鈴木志野, 鈴木志野, 鈴木志野, 清川昌一

    日本微生物生態学会大会(Web)　36th　2023

  13. Microbial Assembly and Adaptation in Deep Subseafloor Sediment

    星野辰彦, 土居秀幸, 伊藤武彦

    日本微生物生態学会大会(Web)　36th　2023

  14. 溶存態有機物濃度による海洋細菌の表面性状変化とナノ粒子付着の関係解明

    山田洋輔, 望月俊昭, 星野辰彦, PATEL Nirav, AZAM Farooq, 福田秀樹, 御手洗哲司

    日本海洋学会大会講演要旨集(Web)　2023　2023

  15. Occurrence of Small Microplastics in Sediment Columnar Samples in the Japan Trench

    田中周平, 實野佳奈, 實野佳奈, 星野辰彦, 西川洋平, 西川洋平, 竹山春子, 竹山春子, 稲垣史生

    日本水環境学会シンポジウム講演集　26th　2023

  16. 日本海溝の海底堆積物における微生物群集の地域多様性

    實野佳奈, 實野佳奈, 西川洋平, 西川洋平, 星野辰彦, 小川雅人, 稲垣史生, 竹山春子, 竹山春子, 竹山春子, 竹山春子

    マリンバイオテクノロジー学会大会講演要旨集　22nd　2022

  17. Genomic analysis of bacterial diversity and function along the Japan Trench

    實野佳奈, 實野佳奈, 西川洋平, 西川洋平, 小川雅人, 星野辰彦, 稲垣史生, 竹山春子, 竹山春子, 竹山春子, 竹山春子

    日本農芸化学会大会講演要旨集(Web)　2022　2022

    ISSN： 2186-7976

  18. Diversity of microbial community in subseafloor sediment

    星野辰彦

    日本地球化学会年会要旨集(Web)　69th　2022

  19. A seafloor survey using multiple methodologies to verify the existence of a village “Kuroda-gori” submerged after a historical Nankai Earthquake in Nomi Bay, Kochi Prefecture

    谷川亘, 村山雅史, 井尻暁, 廣瀬丈洋, 岡崎啓史, 濱田洋平, 浦本豪一郎, 星野辰彦, 山本裕二, 正木裕香, 徳山英一

    日本地球惑星科学連合大会予稿集(Web)　2021　2021

  20. Sedimentary characteristics, age and event of a marine core collected from Uranouchi Bay, Kochi, Japan

    村山雅史, 村山雅史, 谷川亘, 井尻暁, 星野辰彦, 廣瀬丈洋, 捫垣勝哉, 新井和乃, 浦本豪一郎, 近藤康生, 尾嵜大真, 米田穣

    日本地球惑星科学連合大会予稿集(Web)　2021　2021

  21. Underwater geoarchaeological research at the coastal area of Kochi prefecture to reveal the traditional tale of “Kurodagori“

    谷川亘, 徳山英一, 山本裕二, 村山雅史, 田中幸記, 井尻暁, 星野辰彦

    日本地球惑星科学連合大会予稿集(Web)　2020　2020

  22. Characteristics and its significance of marine cores collected from inner Uranouchi Bay, Kochi Prefecture

    村山雅史, 谷川亘, 井尻暁, 星野辰彦, 廣瀬丈洋, 富士原敏也, 北田数也, 捫垣勝哉, 徳山英一, 浦本豪一郎, 新井和乃, 近藤康生, 山本裕二

    日本地球惑星科学連合大会予稿集(Web)　2020　2020

  23. 次世代シーケンシングを用いた核酸定量法の環境影響評価への適用

    猪又健太郎, 北橋倫, 西島美由紀, 星野辰彦, 下田代雅文, 山本啓之

    ブルーアースサイエンス・テク要旨集　2019　2019

  24. 種子島沖海底泥火山の活動度調査速報

    井尻暁, 星野辰彦, 北田数也, 土岐知弘, 村山雅史, 野口拓郎, 芦寿一郎

    日本地質学会学術大会講演要旨　126th　2019

    ISSN： 1348-3935

  25. 微小生命活動の現場を視る-X線マイクロ/ナノCTによる微生物細胞可視化の試み

    諸野祐樹, 浦本豪一郎, 浦本豪一郎, 上杉健太朗, 竹内晃久, 上椙真之, 久保田健吾, 星野辰彦, 稲垣史生

    日本地球惑星科学連合大会予稿集(Web)　2019　2019

  26. 南海地震の災害記録を海底地形・海底面調査から掘り起こす:高知県須崎市野見湾を例に

    谷川亘, 村山雅史, 徳山英一, 田中幸記, 井尻暁, 星野辰彦, 廣瀬丈洋, 北田数也, 富士原敏也

    日本地球惑星科学連合大会予稿集(Web)　2019　ROMBUNNO.SSS15‐P17 (WEB ONLY)　2019

  27. 人工知能による放散虫の自動分類と今後の展望

    板木拓也, 平陽介, 鍬守直樹, 斎藤仁志, 河地正伸, 星野辰彦

    日本古生物学会例会講演予稿集　167th　2018

  28. 海底下微生物の全球的な多様性分布と規定環境要因

    星野辰彦, 土居秀幸, XIAO Nan, 諸野祐樹, 浦本豪一郎, 稲垣史生

    日本地質学会学術大会講演要旨　125th　2018

    ISSN： 1348-3935

  29. 人工知能による微化石群集解析の自動化:南大洋における放散虫Cycadophora davisianaの産出頻度を例に

    板木拓也, 平陽介, 鍬守直樹, 斎藤仁志, 星野辰彦, 池原実

    日本地質学会学術大会講演要旨　125th　2018

    ISSN： 1348-3935

  30. 人工知能を用いた放散虫群集の自動データ取得へ向けた試み

    板木拓也, 平陽介, 鍬守直樹, 斎藤仁志, 星野辰彦

    日本古生物学会年会講演予稿集　2018　2018

  31. 海底下堆積物中に残存する真核生物由来DNAの分布:古代生態系復元への応用の可能性を探る

    星野辰彦, 星野辰彦, XIAO Nan, 浦本豪一郎, 諸野祐樹, 諸野祐樹, RISHI Adhikari, 稲垣史生, 稲垣史生, 稲垣史生

    日本地球惑星科学連合大会予稿集(Web)　2018　2018

  32. 微化石分析の新たなツール:人工知能と連携した顕微鏡画像の自動取得システム

    板木拓也, 河地正伸, 平陽介, 鍬守直樹, 斎藤仁志, 星野辰彦

    日本地球惑星科学連合大会予稿集(Web)　2018　2018

  33. 画像認識・機械学習による微化石の自動分類の評価

    山口龍彦, 萩野恭子, 平陽介, 濱田洋平, 斎藤仁志, 小野寺丈尚太郎, 板木拓也, 星野辰彦, 星野辰彦, 稲垣史生, 稲垣史生, 稲垣史生

    日本地球惑星科学連合大会予稿集(Web)　2018　2018

  34. スーパークリーン技術を用いた実験環境下におけるDNA汚染の影響評価

    寺田武志, 諸野祐樹, 星野辰彦, 鈴木剛人, 佐藤卓広, 湯浅久史, 久保田裕仁, 稲垣史生

    日本地球惑星科学連合大会予稿集(Web)　2018　2018

  35. ランダムシーケンスタグを応用した定量シーケンス(qSeq)法の開発

    星野辰彦, 濱田洋平, 稲垣史生

    日本微生物生態学会大会(Web)　2017　2017

  36. スーパークリーン技術を用いた実験環境下におけるDNA汚染の影響評価

    寺田武志, 諸野祐樹, 星野辰彦, 鈴木剛人, 佐藤卓広, 湯浅久史, 久保田裕仁, 稲垣史生

    日本微生物生態学会大会(Web)　2017　2017

  37. 種子島沖海底泥火山群の間隙水の化学組成鉛直プロファイルから見積もられた泥火山の活動状況

    井尻暁, 濱田洋平, 土岐知弘, 安慶名昂, 星野辰彦, 萩野恭子, 町山栄章, 芦寿一郎, 稲垣史生

    日本地球惑星科学連合大会予稿集(Web)　2017　2017

  38. 自動年代決定AIシステム開発を目指した画像認識・機械学習による微化石分類

    星野辰彦, 星野辰彦, 平陽介, 斎藤仁志, 萩野恭子, 小野寺丈尚太郎, 板木拓也, 山口龍彦, 稲垣史生, 稲垣史生, 稲垣史生

    日本地球惑星科学連合大会予稿集(Web)　2017　2017

  39. Ecophysiology of the deep subseafloor biosphere with new analytical technologies

    諸野祐樹, 諸野祐樹, 寺田武志, 星野辰彦, 星野辰彦, 伊藤元雄, 伊藤元雄, 稲垣史生, 稲垣史生, 稲垣史生

    日本細菌学雑誌(Web)　72　(1)　2017

    ISSN： 1882-4110

  40. 海底下深部生命圏の実態解明に向けた技術開発

    星野辰彦

    環境バイオテクノロジー学会大会プログラム講演要旨集　2016　2016

  41. 種子島沖海底泥火山における水とメタンの起源

    井尻暁, 井尻暁, 土岐知弘, 安慶名昴, 星野辰彦, 星野辰彦, 町山栄章, 芦寿一郎, 稲垣史生, 稲垣史生

    日本地球惑星科学連合大会予稿集(Web)　2016　2016

  42. CCS現場条件を再現するリアクターシステムの開発及びシステムを利用したCO2資源化菌の培養実験

    大友陽子, 井尻暁, 堤正純, 井町寛之, 浦本豪一郎, 星野辰彦, 諸野祐樹, 酒井早苗, 斉藤由美, 谷川亘, 廣瀬丈洋, 稲垣史生, 池川洋二郎

    資源・素材学会北海道支部春季講演会講演要旨集　2015　2015

  43. 土佐清水市竜串海底の石柱群の歴史地震考古学的意義

    谷川亘, 山本裕二, 浦本豪一郎, 濱田洋平, 井尻暁, 星野辰彦, 若木重行, 村山雅史, 廣瀬丈洋, 林原利明, 徳山英一

    日本地質学会学術大会講演要旨　122nd　2015

    ISSN： 1348-3935

  44. クリーン実験環境下におけるDNA汚染の影響評価

    寺田武志, 諸野祐樹, 星野辰彦, 鈴木剛人, 佐藤卓広, 湯浅久史, 久保田裕二, 稲垣史生

    日本微生物生態学会大会(Web)　30th　2015

  45. 薩摩硫黄島長浜湾内に形成された水酸化鉄マウンドに生息する鉄酸化ゼータプロテオバクテリアの生理生態

    星野辰彦, 倉富隆, 諸野裕樹, 掘知行, 清川昌一, 稲垣史生

    日本微生物生態学会大会(Web)　30th　2015

  46. 歴史南海地震災害の理解に向けた高知県沿岸の海底構造物の形成過程

    谷川亘, 山本裕二, 村山雅史, 徳山英一, 若木重行, 廣瀬丈洋, 井尻暁, 星野辰彦

    日本地球惑星科学連合大会予稿集(Web)　2015　2015

  47. 浅海熱水における水酸化鉄マウンドの構造と形成史:鹿児島県薩摩硫黄島長浜湾の例

    倉冨隆, 清川昌一, 池原実, 後藤秀作, 星野辰彦, 池上郁彦, 蓑和雄人

    日本地球惑星科学連合大会予稿集(Web)　2015　2015

  48. 浅海熱水域薩摩硫黄島における,鉄とシリカに富むマウンドの構造とバクテリアとの関連性

    倉冨隆, 清川昌一, 池原実, 後藤秀作, 星野辰彦, 池上郁彦, 蓑和雄人

    日本地質学会学術大会講演要旨　121st　2014

    ISSN： 1348-3935

  49. nxrBを標的としたin situ RCA-FISH法による亜硝酸酸化細菌の探索

    牛木章友, 藤谷拓嗣, 星野辰彦, 常田聡

    日本土壌微生物学会講演要旨集　2014　2014

  50. P24-2 Metabolic potential and community structure of anaerobically respiring microorganisms enriched from deep subseafloor sediments of the Shimokita Coalbed, Japan :

    Hori Tomoyuki, Aoyagi Tomo, Navarro Ronald, Sato Yuya, Itoh Hideomi, Hoshino Tatsuhiko, Morono Yuki, Inagaki Fumio, Kamagata Yoichi

    2014　235-235　2014

    Publisher: 日本微生物生態学会

  51. O34-03 The Cedars蛇紋岩水系に生息する微生物群のメタゲノムおよび生理機能解析(口頭発表)

    鈴木 志野, 石井 俊一, 星野 辰彦, 稲垣 史生, 鎌形 洋一, Gijs Kuenen J., Nealson H. Kenneth

    日本微生物生態学会講演要旨集　2014　124-124　2014

    Publisher: 日本微生物生態学会

  52. O34-07 基質誘導型遺伝子発現解析による海底下微生物の機能遺伝子探索(口頭発表)

    諸野 祐樹, 寺田 武志, 星野 辰彦, 二神 泰基, 稲垣 史生

    日本微生物生態学会講演要旨集　2014　2014

    Publisher: 日本微生物生態学会

  53. O22-07 海底下生命圏の地球横断的解析 : 存在量・多様性を規定する因子の解明(口頭発表)

    星野 辰彦, 諸野 祐樹, 寺田 武志, 稲垣 史生

    日本微生物生態学会講演要旨集　2014　2014

    Publisher: 日本微生物生態学会

  54. P17-2 海底下微生物の統合的生態・機能解析を実現する基盤方法論の確立とその応用(ポスター発表)

    寺田 武志, 諸野 祐樹, 伊藤 元雄, 星野 辰彦, 稲垣 史生

    日本微生物生態学会講演要旨集　2014　176-176　2014

    Publisher: 日本微生物生態学会

  55. シングルセルレベル解析が明らかにする海底下地下生命圏の微生物生態

    星野辰彦

    Institute for Fermentation, Osaka. Research Communications　(27)　2013

    ISSN： 0073-8751

  56. digital PCRによる高精度環境微生物定量

    星野辰彦, 稲垣史生

    日本農芸化学会大会講演要旨集(Web)　2013　2013

    ISSN： 2186-7976

  57. OI-001 プライマー配列に依存しないバイアスレスな微生物群集構造解析 : poly(A)tailing法とRT-PCR法の比較(方法論,口頭発表)

    星野 辰彦, 稲垣 史生

    日本微生物生態学会講演要旨集　29th　(29)　91-91　2013

    Publisher: 日本微生物生態学会

  58. 3A14-3 Analysis of cell wall permeabilizing conditions for highly-sensitive FISH

    FURUKAWA Kazuhiro, HOSHINO Tatsuhiko, TSUNEDA Satoshi, INAMORI Yuhei

    17　72-72　2005

    Publisher: 日本生物工学会

  59. 高感度FISHの水処理生態系への適用における課題

    古川和寛, 星野辰彦, 常田聡, 平田彰, 稲森悠平

    日本水環境学会年会講演集　39th　2005

  60. 水処理プロセスに関与する微生物に高感度FISHを適用するための最適細胞壁消化条件の解析

    古川和寛, 星野辰彦, 常田聡, 稲森悠平

    日本水処理生物学会誌 別巻　(25)　2005

    ISSN： 0910-6766

  61. rRNAを標的とした環境微生物DNAマイクロアレイ操作条件の最適化

    松本純平, 星野辰彦, 常田聡, 平田彰, 珠坪一晃, 浦川秀敏

    日本水環境学会年会講演集　39th　2005

  62. 高感度FISHを様々な細菌に適用する際の細胞壁消化条件の検討(モニタリング,口頭発表)

    星野辰彦, 古川和寛, 常田聡, 稲森悠平

    日本微生物生態学会講演要旨集　(21)　2005

  63. T-RFLP法による排水処理細菌叢の迅速モニタリング

    星野 辰彦, 寺原 猛, 常田 聡

    用水と廃水　46　(5)　408-412　2004/05

    Publisher: 産業用水調査会

    ISSN： 0513-5907

  64. T-RFLP法およびクローニング法の併用による排水処理脱窒細菌群の同定

    星野辰彦, 寺原猛, 常田聡, 平田彰, 稲森悠平

    日本水処理生物学会誌 別巻　(24)　2004

    ISSN： 0910-6766

  65. T-RFLP法による生物学的排水処理反応槽内のバイオコミュニティー変遷モニタリング

    星野辰彦, 寺原猛, 常田聡, 平田彰, 稲森悠平

    日本水環境学会年会講演集　38th　2004

  66. T-RFLP法による排水処理微生物叢の簡易・迅速モニタリング

    寺原猛, 星野辰彦, 常田聡, 平田彰

    化学工学会年会研究発表講演要旨集　69th　2004

  67. T-RFLP法による排水処理細菌叢の迅速モニタリング

    星野辰彦, 寺原猛, 常田聡, 平田彰, 稲森悠平

    日本水処理生物学会誌 別巻　(23)　2003

    ISSN： 0910-6766

  68. T-RFLP法による排水処理微生物叢の簡易・迅速モニタリング技術の開発

    稲森悠平, 寺原猛, 星野辰彦, 常田聡, 平田彰

    日本水環境学会年会講演集　37th　2003

  69. T-RFLP法を用いた機能遺伝子発現プロファイルの解析

    星野辰彦, 寺原猛, 常田聡, 平田彰, 稲森悠平

    日本水環境学会年会講演集　37th　2003

  70. 機能に基づいた排水処理微生物検出手法の開発

    星野 辰彦, 常田 聡, 平田 彰, 稲森 悠平

    日本水処理生物学会誌. 別巻 = Journal Japan Biological Society of Water and Waste　22　(22)　40-40　2002/10/15

    ISSN： 0910-6766

  71. T-RFLP法による排水処理微生物叢の解析評価

    稲森悠平, 寺原猛, 星野辰彦, 常田聡, 平田彰

    日本水処理生物学会誌 別巻　(22)　2002

    ISSN： 0910-6766

  72. 分子生物学的手法を用いた生物学的リン除去プロセスにおける有用微生物の評価・解析

    近藤貴志, 星野辰彦, 吉江幸子, えび江美孝, 常田聡, 平田彰, 稲森悠平

    日本水処理生物学会誌 別巻　(22)　2002

    ISSN： 0910-6766

  73. B-11 バイオフィルム内における機能遺伝子(amoA)のin situ検出(群集構造解析,B会場,口頭発表)

    星野 辰彦, 永野 達生, 野田 尚宏, 常田 聡, 平田 彰, 稲森 悠平

    日本微生物生態学会講演要旨集　(17)　61-61　2001

    Publisher: 日本微生物生態学会

  74. 硝化グラニュール形成微生物の分子生物学的手法による解析

    星野辰彦, 永野達生, 野田尚宏, 常田聡, 平田彰, 稲森悠平

    日本水処理生物学会誌 別巻　(21)　2001

    ISSN： 0910-6766

  75. 嫌気好気循環生物膜法における生活排水中の環境ホルモン様物質の除去特性の評価

    稲森悠平, 星野辰彦, 平田彰, 常田聡, 野田尚宏, 西村浩

    日本水環境学会年会講演集　34th　2000

  76. The evaluation of removal character of exogenous endocrine disruptor chemicals in daily life drainage by anaerobic and aerobic circulation biofilm process.

    稲森悠平, 星野辰彦, 西村浩, 常田聡, 平田彰, 野田尚宏

    日本水処理生物学会誌　1999

    ISSN： 0910-6758

Show all ︎Show first 5

Presentations 130

  1. Physical Property Constraints on Past Slip-to-the-Trench Megathrust Earthquakes from Trench-Fill Sediments, Central Japan Trench

    橋本 善孝, Shorrock Anthony, Hanifah Dina, Bowden Stephe, Chang Yu-Chun, Cornard Pauline, Diz Paula, 江口 暢久, 藤島 誠也, 福地 里菜, Gea MariaGea, 星野 辰彦, Hovikosko Jussi, Huang Tzu-Ha, 塚, 石澤 尭, 北里 洋, März Christia, Muthre Mishelle, 奥津 なつみ, Pizer Charlotte, Test Claudio, 里口 保, Stipp Michael, Stewart Margare, Strachan Lorn, Toczko Sea, Xiao Wenjie, 山岸 昇玄, 山本 裕二, 横山 由, Zabel Matthia, 前田 玲奈, 池原, Strasser Michael

    2026/05/26

  2. 琉球海溝海底泥火山群の潜航調査（KS-25-15次航海）速報

    井尻 暁, 土岐 知弘, 星野 辰彦, 大塚 宏徳, 波々伯部, 自見 直人, 田中 翔, 河野 龍馬

    JpGU-AGU Joint Meeting 2026　2026/05/27

  3. 津波沈水遺跡と火山灰埋没遺跡の統合解析による日本列島の生存史解明に向けて

    山本 裕二, 井尻 暁, 伊藤 元雄, 浦本 豪一郎, 奥村 知世, 木村 淳, 佐川 拓也, 谷川 亘, 中西 諒, 馬場 基, 星野 辰彦, 山崎 新太郎

    JpGU-AGU Joint Meeting 2026　2026/05/24

  4. Seasonal microbial community dynamics in the Ariake Sea responding to coastal hypoxia and algal blooms

    2026/05/28

  5. 水底考古遺跡に対する柱状試料解析の有効性：桧原湖湖底遺跡の事例

    谷川 亘, 山本 裕二, 三宅 尚, 山本 哲也, 井尻 暁, 星野 辰彦, 木村 淳

    JpGU-AGU Joint Meeting 2026　2026/05/24

  6. 人新世を掘る：東京湾人工地層の地下地球科学的進化

    谷川亘, 井尻暁, 星野辰彦, 朝日博史, 山本裕二

    J-DESCシンポジウム「新たなICDP掘削提案に向けてー成功を共有し、次の挑戦へー　2025/11/29

  7. 海洋一粒子の微生物叢･機能･成分の統合分析

    星野辰彦, 山田洋輔, 長野由梨子, 菊池早希子, 稲垣史生

    日本海洋学会2025年度秋季大会　2025/09/22

  8. 海底下堆積物における微生物ゲノムの進化的静止

    星野 辰彦, 佐久間 敦丈, 伊藤 武彦, 土居 秀幸, 稲垣 史生

    2025/09/09

  9. 北部琉球海溝海底泥火山群からの溶存有機態炭素の放出

    吉崎 結衣, 星野 辰彦, 乙坂 重嘉, 山下 洋平, 松井 洋平, 川口 慎介, 山地 一代, 竹内 誠, 岡村 慶, 野口 拓郎, 井尻 暁

    日本地球惑星科学連合2025年大会　2025/05/25

  10. 海洋深層水を用いた微生物培養によるバイオMn酸化物の合成および有用元素の回収

    菊池 早希子, 星野 辰彦, 宮崎 征行, 秋田 もなみ, 井町 寛之

    日本地球惑星科学連合2025年大会　2025/05/27

  11. 海洋沈降粒子一粒子分析スキームの構築

    星野辰彦, 山田洋輔, 高橋亮, 稲垣史生

    日本地球惑星科学連合2025年大会　2025/05/27

  12. 海洋粒子の一粒子レベル解析による微生物群集組成と代謝機能ポテンシャル

    星野辰彦, 山田洋輔, 長野由梨子, 稲垣史生

    第25回マリンバイオテクノロジー学会大会　2025/05/25

  13. 岩石内地下水圏環境に生命は存続可能か？世界最古の地下水圏環境における微生物学的解析 International-coauthorship

    鈴木志野, 星野辰彦, Oliver Warr, 石井俊一, 稲垣史生, Barbara Sherwood Lollar

    日本微生物生態学会 第37回大会　2024/10/30

  14. 日本海溝超深海微生物群集の地理的空間分布

    星野辰彦, 西川洋平, 竹山春子, 稲垣史生

    日本微生物生態学会 第37回大会　2024/10/29

  15. Discharge and dispersal of deep sub-seafloor microorganisms from mud volcanoes into ocean

    星野 辰彦, 土岐 知弘, 野口 拓郎, 芦 寿一郎, 村山 雅史, 井尻 暁

    日本地球惑星科学連合2024年大会　2024/05/26

  16. 海底泥火山群からの溶存態有機物および栄養塩の放出

    吉崎 結衣, 岡村 慶, 野口 拓郎, 八田 万有美, 星野 辰彦, 竹内 誠, 板木 拓也, 遠山 早紀, 小林 祐大, 塚原 佳穂, 山下 廉太郎, 乙坂 重嘉, 井尻 暁

    日本地球惑星科学連合2024年大会　2024/05/26

  17. KH-23-4航海で採取された西南海トラフおよび琉球海溝北部の泥火山噴出物の空隙率

    福地 里菜, 趙, 陽, 井尻 暁, 板木 拓也, 吉本 剛瑠, 山口 飛鳥, 村山 雅史, 大塚 宏徳, 土岐 知弘, 星野 辰彦, KH23-4乗船研究者

    2024/05/26

  18. 喜界島～日向灘における泥火山分布 -白鳳丸KH-23-4 地物調査速報- International-presentation

    大塚 宏徳, 井尻 暁, 中西 諒, 福地 里菜, 星野 辰彦, 土岐 知弘, 板木 拓也, 北田 数也, 浅田 美穂, 中尾 眞子, 鈴木 由布, 波多野 泰成, 松下 誠, 辰巳 寛二, 吉本 剛瑠, KH-23-4乗船研究者

    日本地球惑星科学連合2024年大会　2024/05/26

  19. 多様性と存在量を同時に推定する環境DNA解析手法の提案

    2024/03/24

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    Ks00068433

  20. 薩摩硫黄島の鉄リッチなチムニーに生息する電気微生物の網羅的解析 Invited

    2023/11/30

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    Ks00069173

  21. 海底下深部堆積物における微生物叢の成り立ちと適応

    2023/11/28

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    Ks00068580

  22. Comparative single-cell genomics of Atribacterota JS1 in the Japan Trench hadal sedimentary biosphere

    Jitsuno Kana, Hoshino Tatsuhiko, Nishikawa Yohei, Kogawa Masato, Mineta Katsuhiko, Inagaki Fumio, Takeyama Haruko, IODP Expedition 386 Scientists

    13th Asia Pacific Marine Biotechnology Conference　2023/10/03

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    Ks00068618

  23. 溶存態有機物濃度による海洋細菌の表面性状変化とナノ粒子付着の関係解明

    2023/09/25

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    Ks00068719

  24. 日本海溝における底質柱状試料中の微小マイクロプラスチックの存在実態調査

    2023/09/20

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    Ks00067311

  25. 日本海溝に生息する超深海底微生物群集の多様性及び代謝機能の解明

    2023/09/03

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    Ks00068616

  26. 日本海溝に生息する超深海微生物群の遺伝的機能の解明

    2023/05/28

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    Ks00068615

  27. Mechanism of community assembly in the subseafloor biosphere Invited

    Tatasuhiko Hoshino, Rei Kajitani

    Japan Geoscience Union Meeting 2023　2023/05/22

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    Ks00068818

  28. 高知県浦ノ内湾堆積物中の環境DNA解析による生物変遷分析

    2023/03/31

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    Ks00066443

  29. 高知県浦ノ内湾堆積物中に残存する環境DNAによる生物相変遷の試み

    2023/03/16

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    Ks00066175

  30. 海底下深部微生物群の生態と進化

    2023/02/17

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    Ks00066450

  31. 温暖化以前の海洋微⽣物群集復元の試み -PCR発明前のフィルターからの環境DNA分析-

    2022/11/19

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    Ks00065157

  32. 海底下堆積物の微生物多様性 Invited

    2022/09/08

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    Ks00064939

  33. Marine phytoplankton biogeography before the recent global ocean warming

    Kyoko Hagino, Yurika Ujiie, Masao Iwai, Tatsuhiko Hoshino, Richard Jordan, Masayuki Utsunomiya, Yuichiro Tanaka, Jeremy R. Young, El Mahdi Bend, Ian Robert

    18th International Nannoplankton Association Meeting　2022/08/30

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    Ks00064870

  34. Biogeographical distribution and diversity of hadal microbial communities along a ~500 km-transect of the Japan Trench (IODP Expedition 386)

    Kana Jitsuno, Tatsuhiko Hoshino, Yohei Nishikawa, Masato Kogawa, Fumio Inagaki, Haruko Takeyama, Michael Strasser, Ken Ikehara, Jeremy Everest , Lena Maeda, and IODP Expedition 386 Scientists

    18th International Symposium on Microbial Ecology (ISME18)　2022/08/16

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    Ks00065163

  35. 日本海溝の海底微生物の地域多様性と代謝機構の解析

    2022/06/29

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    Ks00065164

  36. 海底下泥火山微生物群集の起源と直上海水との相互作用

    2022/06/01

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    Ks00064788

  37. 日本海溝の海底堆積物における微生物群集の地域多様性

    2022/05/29

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    Ks00064790

  38. 種子島沖海底泥火山群の総合調査（KS-２１-２７次航海）速報

    2022/05/24

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    Ks00064789

  39. ゲノム情報を用いた日本海溝の海底微生物の多様性と機能の解明

    2022/03/18

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    Ks00064787

  40. 全球規模の海底堆積物を用いた古環境DNA分析の予察と地球システム科学への適用可能性について

    2021/11/20

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    Ks00063682

  41. Global microbial diversity in subseafloor sediment Invited

    HOSHINO TATSUHIKO

    IC-DLI Webinar Series for Deep Life Studies　2021/11/18

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    Ks00063683

  42. 浦ノ内湾から採取された海洋コア堆積物の特徴と年代およびイベント堆積物について

    2021/06/05

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    Ks00064054

  43. 南海地震水没伝承「黒田郡」の痕跡発掘に向けた沿岸域海底調査：高知県須崎市野見湾を例に Invited

    2021/06/04

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    Ks00064052

  44. Preliminary investigation on geochemical distribution of heavy metals in Uranouchi Inlet Bay sediments during the the Anthropocene Epoch

    村山 雅史、谷川 亘、星野 辰彦、井尻 暁、廣瀬 丈洋、新井 和乃、浦本 豪一郎、近藤 康生

    JpGU2021 / MIS03-P02　2021/06/03

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    Ks00064053

  45. 高知県浦ノ内湾奥から採取された海洋コアの堆積物の特徴とその意義

    2020/07/12

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    Ks00064066

  46. 黒田郡伝承の謎にせまる高知県沿岸部海底調査：概要紹介

    2020/07/12

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    Ks00064063

  47. Global diversity of subseafloor sedimentary microbiome

    Tatsuhiko Hoshino, Hideyuki Doi, Go-Ichiro Uramoto, Lars Wörmer, Rishi R. Adhikari, Nan Xiao1, Yuki Morono, Steven D'Hondt, Kai-Uwe Hinrichs, Fumio Inagaki

    Deep Carbon 2019　2019/10/25

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    Ks00059884

  48. 種子島沖海底泥火山の活動度調査速報

    2019/09/24

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    Ks00059731

  49. 南海地震の災害記録を海底地形・海底面調査から掘り起こす：高知県須崎市野見湾を例に

    2019/05/28

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    Ks00059764

  50. Interaction between submarine landslides and subseafloor sedimentary microbiome: Chikyu SCORE Expedition 910 off Cape Erimo

    2019/05/27

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    Ks00059881

  51. 次世代シーケンシングを用いた核酸定量法の環境影響評価への適用

    2019/02/21

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    Ks00056970

  52. Exploration of the sedimentary biosphere in Bengal Fan (IODP Exp. 354)

    Adhikari Rishi, Heuer Verena, Elvert Marcus, Hoshino Tatsuhiko, Inagaki Fumio, Kallmeyer Jens, Morono Yuki, Wormer Lars, Hinrichs Kai-Uwe

    AGU Fall Meeting 2018　2018/12/11

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    Ks00055885

  53. Global diversity of subseafloor microbial community

    Hoshino Tatsuhiko, Doi Hideyuki, Wormer Lars, Morono Yuki, D'Hondt Steven, Hinrichs Kai-Uwe, Inagaki Fumio

    AGU Fall Meeting 2018　2018/12/11

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    Ks00055884

  54. A new tool for microfossil analysis in the Southern Ocean -automatic image collector equipped with deep learning-

    Itaki Takuya, Taira Yosuke, Kuwamori Naoki, Saito Hitoshi, Hoshino Tatsuhiko, Ikehara Minoru

    AGU Fall Meeting 2018　2018/12/11

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    Ks00055883

  55. クリーンフィルターはDNAエアロゾルを止められるのか？汚染除去効果の検証

    2018/12/08

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    Ks00055919

  56. 海底下深部堆積物中の真核生物環境DNAによる古代生態系の復元

    2018/09/29

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    Ks00055656

  57. 微小生命活動の現場を視る−X線マイクロ/ナノCTによる微生物細胞可視化の試み X-ray computed nano-tomography for visualizing microbial cells and its surrounding environments

    2018/07/12

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    Ks00055559

  58. 海底下深部生命圏から海水中への泥火山を通した微生物の拡散

    2018/07/12

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    Ks00055423

  59. 微化石分析の新たなツール：人工知能と連携した顕微鏡画像の自動取得システム

    2018/05/23

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    Ks00055229

  60. Distribution of Aerobic Microbial Activities in Ultra-Oligotrophic Sediments of the South Pacific Gyre

    YUKI MORONO, TAKESHI TERADA, MOTOO ITO, STEVEN D'HONDT, TATSUHIKO HOSHINO, FUMIO INAGAKI

    JpGU2018　2018/05/22

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    Ks00055555

  61. 海底下堆積物中に残存する真核生物由来DNAの分布：古代生態系復元への応用の可能性を探る

    2018/05/22

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    Ks00055230

  62. 画像認識・機械学習による微化石の自動分類の評価

    2018/05/20

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    Ks00055231

  63. 人工知能による放散虫の自動分類と今後の展望

    2018/02/02

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    Ks00053587

  64. Global microbial diversity in subseafloor sediment

    Tatsuhiko Hoshino, Hideyuki Doi, Lars Wormer, Yuki Morono, Kai-Uwe Hinrichs, Fumio Inagaki

    The international society for subsurface microbiology 2017 conference　2017/11/09

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    Ks00053564

  65. オープンクリーンシステムを用いた実験環境下におけるDNA汚染の影響評価

    2017/08/30

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    Ks00053895

  66. ランダムシーケンスタグを応用した定量シーケンス（qSeq）法の開発

    2017/08/30

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    Ks00052421

  67. Metabolic diversity of hyperalkaliphilic microbial communities associated with serpentinization at The Cedars Invited

    SHINO SUZUKI, SHUN’ICHI ISHII, TATSUHIKO HOSHINO, FUMIO INAGAKI, J.GIJS KUENEN, KENNETH H. NEALSON

    Goldschmidt 2017　2017/08/18

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    Ks00053541

  68. The global survey of subseafloor sedimentary microbiomes

    TATSUHIKO HOSHINO, HIDEYUKI DOI, LARS WORMER, YUKI MORONO, STEVEN D’HONDT, KAI-UWE HINRICHS, FUMIO INAGAKI

    Goldschmidt2017　2017/08/18

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    Ks00052420

  69. The deep biosphere in Bengal Fan sediments (IODP Exp. 354)

    R.R. ADHIKARI, V.B. HEUER, M. ELVERT, T. HOSHINO, F. INAGAKI, J. KALLMEYER, A. KITTE, L. WORMER, K.-U. HINRICHS

    Goldschmidt2017　2017/08/17

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    Ks00052418

  70. Secondary Methanogenesis in Dormant Submarine Mud Volcano off Tanegashima Island, Japan

    Ijiri A, Toki T, Agena K, Hoshino T, Hagino K, Hamada Y, Machiyama H, Ashi J & Inagaki F

    Goldschmidt 2017　2017/08/15

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    Ks00054901

  71. Characterization of Deep Marine Subsurface Fungi from South Pacific Gyre Sediments

    Morgan Sobol, Mayra Rodriguez, Tatsuhiko Hoshino, Fumio Inagaki, Brandi Kiel Reese

    Astrobiology Graduate Conference 2017　2017/06/05

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    Ks00052322

  72. 自動年代決定AIシステム開発を目指した画像認識・機械学習による微化石分類

    2017/05/24

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    Ks00052417

  73. お花見メタゲノム プロジェクト結果報告

    2017/05/22

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    Ks00052323

  74. 種子島沖海底泥火山群の間隙水の化学組成鉛直プロファイルから見積もられた泥火山の活動状況

    2017/05/20

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    Ks00054898

  75. The global biogeography of subseafloor sedimentary microbiomes

    Hoshino Tatsuhiko,Fumio Inagaki, Woemer L, Morono Y, D'Hondt S, Hinrichs K-U

    DCO international Science Meeting　2017/03/23

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    Ks00052325

  76. Recent development in the use of per fluorocarbon tracers for contamination monitoring in ocean drilling

    Lever Mark, Torti A, Hoshino Tatsuhiko, Inagaki Fumio, Jorgensen BB, Kevorkian RT, Liu C, Marshall IPG

    DCO international Science Meeting　2017/03/23

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    Ks00052324

  77. Biosphere in subseafloor environment and its ecophysiology Invited

    2017/03/20

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    Ks00050292

  78. Subseafloor life and carbon cycling in the Bengal Fan (IODP Exp. 354)

    R.R. Adhikari, V. B. Heuer, L. Wörmer, S. Jabinski, T. Hoshino, F. Inagaki, A. Kitte, J. Kallmeyer, K.-U. Hinrichs

    IODP/ICDP Colloquium 2017　2017/03/14

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    Ks00051985

  79. Preliminary results from IODP Expedition 370 (Temperature Limit of the Deep Biosphere off Muroto, T-LIMIT)

    V.B. HEUER, F. INAGAKI, Y. MORONO, Y. KUBO, S. HENKEL, F. SCHUBOTZ, B. VIEHWEGER, AND THE IODP EXPEDITION 370 SCIENTISTS

    IODP/ICDP Kolloquium 2017　2017/03/14

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    Ks00051065

  80. IODP室戸沖限界生命圏掘削調査（T-Limit）の概要について

    2017/03/02

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    Ks00052186

  81. 「ちきゅう」によるIODP室戸沖限界生命圏掘削調査（T-Limit）のオペレーション概要

    2017/03/02

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    Ks00052185

  82. Distribution of Aerobic Microbial Activities in Ultra-Oligotrophic Sediments of the South Pacific Gyre

    YUKI MORONO, TAKESHI TERADA, MOTOO ITO, TATSUHIKO HOSHINO, STEVEN D'HONDT, FUMIO INAGAKI

    ISME16　2016/08/22

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    Ks00050425

  83. Assessing Abundance and Relevance of Bacterial Endospores in the Marine Subsurface

    Woermer Lars , HOSHINO TATSUHIKO , Viehweger B , Meador T , Gajendra N , Stern B , INAGAKI FUMIO , Hinrichs Kai-Uwe

    Goldschmidt 2016　2016/06/30

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    Ks00050445

  84. Global distribution pattern of aerobic and anaerobic communities in deep subseafloor sediments

    HOSHINO TATSUHIKO , Woemer Lars , Viehweger B , XIAO NAN , MORONO YUKI , Kai-Uwe Hinrichs , INAGAKI FUMIO

    Goldschmidt 2016　2016/06/30

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    Ks00050444

  85. Metabolic Diversity of Microorganisms Associated with Active Subsurface Serpentinization

    SHINO SUZUKI, SHUN'ICHI ISHII, TATSUHIKO HOSHINO, FUMIO INAGAKI, JOSE MANUEL MARQUES, KENNETH H. NEALSON

    Goldschmidt 2016　2016/06/29

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    Ks00051945

  86. Distribution of Aerobic Microbial Activities in Ultra-Oligotrophic Sediments of the South Pacific Gyre

    YUKI MORONO, TAKESHI TERADA, MOTOO ITO, TATSUHIKO HOSHINO, STEVEN D'HONDT, FUMIO INAGAKI

    Goldschmidt 2016　2016/06/27

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    Ks00050284

  87. 海底下生物圏の解明を目指した技術開発 Invited

    2016/06/13

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    Ks00050435

  88. 海底泥火山から水圏への海底下微生物群集の拡散

    2016/05/24

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    Ks00050697

  89. 種子島沖海底泥火山における水とメタンの起源

    2016/05/24

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    Ks00050384

  90. Ecophysiology of iron-oxidizing Zetaproteobacteria in an iron oxyhydroxide mound in a shallow marine environment at Satsuma Iwo-jima, Japan.

    HOSHINO TATSUHIKO, Kuratomi Takashi, MORONO YUKI, Hori Tomoyuki, Kiyokawa Shoichi, INAGAKI FUMIO

    2015 AGU Fall meeting　2015/12/14

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    Ks00046981

  91. 海底下生命のセンサス：海底下微生物群集の空間的分布と地球環境との相互作用の解明

    2015/11/07

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    Ks00046979

  92. 薩摩硫黄島長浜湾内に形成された水酸化鉄マウンドに生息する鉄酸化ゼータプロテオバクテリアの生理生態

    2015/10/19

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    Ks00048763

  93. 歴史南海地震の理解にむけた高知県西部海底構造物の地質学的研究

    2015/09/21

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    Ks00048646

  94. 土佐清水市竜串海底の石柱群の歴史地震考古学的意義

    2015/09/12

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    Ks00048639

  95. 地下深部微生物群集構造解析

    2015/07/02

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    Ks00046978

  96. 歴史南海地震災害の理解に向けた高知県沿岸の海底構造物の形成過程

    2015/05/27

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    Ks00048640

  97. 浅海熱水における水酸化鉄マウンドの構造と形成史：鹿児島県薩摩硫黄島長浜湾の例

    2015/05/27

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    Ks00046975

  98. 薩摩硫黄島の浅海性熱水マウンドにおける鉄酸化微生物群集の分子生態学的・鉱物学的特徴

    2015/05/26

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    Ks00046974

  99. Microbial life associated with active subsurface serpentinization at “The Cedars” springs

    SUZUKI SHINO, ISHII SHUNICHI, HOSHINO TATSUHIKO, Morrill Penny L. , INAGAKI FUMIO, Kuenen J. Gijs, Nealson Kenneth H.

    Deep Life Community Lisbon, Portugal Meeting　2015/05/08

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    Ks00047055

  100. Extensive quantification of the genes in subseafloor sediments using microfluidic digital PCR

    HOSHINO TATSUHIKO, INAGAKI FUMIO

    Deep Life Community Science Meeting　2015/05/06

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    Ks00046973

  101. Digging out DNA sequences of indigenous microbial community in the ultra-deep subsurface sediment cores

    HOSHINO TATSUHIKO, Methe Barbara , INAGAKI FUMIO

    IODP Expedition 337, 2nd post cruise meeting　2015/04/13

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    Ks00046972

  102. The structure of iron-oxyhydroxide mounds affected by iron-oxidizing bacteria at shallow submarine hydrothermal vent in Satsuma Iwo-Jima

    Kuratoi Takashi, Kiyokawa Shoichi , Ikehara Minoru , Shusaku Goto , HOSHINO TATSUHIKO, INAGAKI FUMIO, Minowa Yuto

    AGU Fall Meeting 2014　2014/12/18

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    Ks00044228

  103. エアロゾルDNAフリー環境の構築とその評価

    2014/11/02

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    Ks00043312

  104. nxrBを標的としたin situ RCA-FISH法による亜硝酸酸化細菌の探索

    2014/10/24

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    Ks00043769

  105. Metabolic potential and community structure of anaerobically respiring microorganisms enriched from deep subseafloor sediments of the Shimokita Coalbed, Japan

    2014/10/24

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    Ks00043768

  106. 基質誘導型遺伝子発現解析による海底下微生物の機能遺伝子探索

    2014/10/24

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    Ks00043318

  107. 蛇紋岩水系に生息する微生物群のメタゲノムおよび生理機能解析

    2014/10/23

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    Ks00043767

  108. 海底下生命圏の地球横断的解析−存在量・多様性を規定する因子の解明−

    2014/10/23

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    Ks00043765

  109. Development of Analytical Technologies for Low-Biomass Microbial Communities in the Subseafloor Sedimentary Biosphere

    64. Morono, Y., Terada, T., Ito, M., Hoshino, T., and Inagaki, F.

    Ninth International Symposium on Subsurface Microbiology　2014/10/06

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    Ks00043295

  110. 浅海熱水域薩摩硫黄島における、鉄とシリカに富むマウンドの構造解析とバクテリアとの関連性

    2014/09/11

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    Ks00043760

  111. Detection of nxrB genes by in situ RCA-FISH to identify unrecognized nitrite-oxidizing microorganisms in environments

    Ushiki Norisuke, Fujitani Hirotsugu, HOSHINO TATSUHIKO, Tsuneda Satoshi

    15th International Symposium on Microbial Ecology　2014/08/28

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    Ks00043753

  112. Geographical survey of microbiome in the deep subseafloor sediments

    HOSHINO TATSUHIKO, MORONO YUKI, INAGAKI FUMIO

    15th International Symposium on Microbial Ecology　2014/08/25

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    Ks00043749

  113. Metabolic potential and community structure of anaerobically respiring microorganisms enriched from deep subseafloor sediments of the Shimokita Coalbed, Japan

    Hori Tomoyuki, Aoyagi Tomo , Navarro R , Sato Y , Itoh H , HOSHINO TATSUHIKO, MORONO YUKI, INAGAKI FUMIO, Kamagata Yoichi

    15th International Symposium on Microbial Ecology　2014/08/25

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    Ks00043748

  114. Microfluidic digital PCR for less-biased quantification of microbial communities in marine subsurface environments

    HOSHINO TATSUHIKO

    15th International Symposium on Microbial Ecology　2014/08/25

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    Ks00043745

  115. Geographical Molecular Analysis of the Deep Subseafloor Sedimentary Microbiomes

    HOSHINO TATSUHIKO, MORONO YUKI, INAGAKI FUMIO

    9th International Symposium on Subsurface Microbiology　2014/08/06

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    Ks00043754

  116. 熱アルカリ細胞溶菌法による海底下微生物群集構造解析

    2014/04/30

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    Ks00043319

  117. The structure of iro- and silica-rich mounds at hydrothermal environment in shallow marine, Satsuma Iwo-Jima, Kikai caldera, Japan

    Kuratomi Takashi, Kiyokawa Syoichi , Ikehara Minoru , Goto Syusaku , HOSHINO TATSUHIKO, Ikegami Fumihiko , Minowa Yuto

    Japan Geoscience Union Meeting 2014　2014/04/29

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    Ks00043762

  118. The structure of iron-hydroxide mounds at hydrothermal environment in shallow marine, Satsuma Iwo-Jima, Kikai caldera, Japan

    Kuratomi Takashi, Kiyokawa Syoichi, Ikehara Minoru, Goto Syuuraku, HOSHINO TATSUHIKO, Ikegami Fumihiko, Minowa Yuto

    2013 AGU Fall Meeting　2013/12/10

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    Ks00041228

  119. Technological challenges for the advanced study of deep subseafloor life

    Y. Morono, T. Terada, M. Ito T. Hoshino and F. Inagaki

    International Astrobiology Workshop 2013 (6th Japan Astrobiology Network Workshop (JABN6))　2013/11/30

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    Ks00039220

  120. Global census of microbial life in marine subsurface sediments

    HOSHINO TATSUHIKO, TSUTSUMI MASAZUMI, MORONO YUKI, INAGAKI FUMIO, INAGAKI FUMIO

    アストロバイオロジー国際会議　2013/11/29

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    Ks00040423

  121. プライマー配列に依存しないバイアスレスな微生物群集構造解析:poly(A) tailing法とRT-PCR法の比較

    2013/11/23

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    Ks00041217

  122. 強アルカリ条件を用いた海底下試料からの効率的核酸抽出法の確立とその評価

    2013/10/27

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    Ks00041223

  123. An improved hot-alkaline DNA extraction method for high cell-lysis efficiency of subseafloor microbial communties

    Terada, T., Morono, Y., Hoshino, T., and Inagaki, F.

    Goldschmidt2013　2013/08/26

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    Ks00041225

  124. Technological challenges for the advanced study of subseafloor life Invited

    YUKI MORONO, TAKESHI TERADA, MOTOO ITO, TATSUHIKO HOSHINO, AND FUMIO INAGAK

    Goldschmidt2013　2013/08/26

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    Ks00038153

  125. A Global Molecular Ecological Survey of Subseafloor Microbial Communities

    HOSHINO TATSUHIKO, HOSHINO TATSUHIKO, TSUTSUMI MASAZUMI, MORONO YUKI, INAGAKI FUMIO

    Goldschmidt 2013　2013/08/25

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    Ks00041226

  126. The Challenge of Contamination in Deep Life Exploration

    Morono Y., Lever M.A., Hoshino T., and Inagaki F.

    The CHIKYU+10 Workshop　2013/04/22

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    Ks00041227

  127. digital PCRによる高精度環境微生物定量

    2013/03/25

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    Ks00035974

  128. 薩摩硫黄島における浅海熱水環境中での鉄とシリカに富むマウンドの構造解析

    2013/02/23

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    Ks00041221

  129. 海底下に埋もれたサンゴ化石は微生物の住処となっているのか？ −NGSを用いた微生物群集構造の比較解析−

    2012/05/24

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    Ks00035969

  130. Digital PCR法による環境微生物の高精度遺伝子定量

    2011/11/28

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    Ks00030751

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Industrial Property Rights 4

  1. 標的核酸の定量方法及びそのためのキット

    星野 辰彦

    Property Type: Patent

  2. 微生物を含有する充填用組成物及びその利用

    廣瀬丈洋, 谷川亘, 星野辰彦, 井尻暁

    Property Type: Patent

  3. 解析装置、地層年代推定装置、解析方法、地層年代推定方法、およびプログラム

    星野辰彦, 平陽介, 鍬守直樹, 小野寺丈尚太郎, 山口龍彦, 富岡恭子, 板木拓也

    Property Type: Patent

  4. 標的核酸の定量方法及びそのためのキット

    星野 辰彦

    特許第6284137号

    Property Type: Patent

Research Projects 26

  1. Understanding the Impact of Accelerated Ocean Warming on Marine Microbial Ecosystems

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Japan Agency for Marine-Earth Science and Technology

    2025/04 - 2029/03

  2. Geosphere-hydrosphere-biosphere connected by submarine mud volcanism: Diffusion processes of deep life and carbon, and their effects on the ocean

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Kobe University

    2024/04 - 2029/03

  3. 海洋炭素フラックス予測のミッシングピースを埋める一粒子網羅的解析への挑戦

    星野 辰彦, 山口 飛鳥, 山田洋輔

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(開拓)

    Institution: 国立研究開発法人海洋研究開発機構

    2024/06 - 2028/03

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    R6年度は、フィルター上に捕捉した海洋粒子の顕微鏡下での形態観察、個々の海洋粒子のレーザーマイクロダイセクションによる切り出し、DNA増幅のプロトコールを確立するべく検討を行ってきた。レーザーマイクロ第セクションによる個々の粒子のサンプリングは、粒子を捕捉するフィルターの材質によってレーザーによる切り出し効率が変わってくるため、複数の材質・メーカーのフィルターを試した結果、ポリカーボネート製のフィルターが適していることがわかった。また、切り出した海洋粒子から効率よくDNAの増幅を行うためには、微生物の細胞を消化する必要がある。細胞を消化する手段として、アルカリ(KOH)溶液による処理、あるいは酵素（プロテナーゼK、リゾチーム、または複数の酵素）による処理が考えられる。これらの方法で海洋粒子を処理したのちに定量PCRやアンプリコンシーケンスを実施し、それぞれの処理方法を評価した。その結果、酵素処理と比較してKOHで処理したサンプルにおいてDNAがよく増幅されるとともに、多様性の高い微生物群集が得られることがわかった。 また、個々の海洋粒子の元素組成の解析に向けて、海洋粒子を常温常圧かつ湿潤条件で元素分析を行うために、東京大学大気海洋研究所に既設の微小部蛍光X線分析装置（μXRF）にHeパージ機能を追加装備した。また、生物源粒子や海洋堆積物などのマッピング分析・定量分析を行い、さまざまな空間スケールにおける分析手法の確立に努めた。

  4. 海洋深層水を用いた微生物培養による鉱物資源の創出

    菊池 早希子, 星野 辰彦

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 国立研究開発法人海洋研究開発機構

    2025/06/27 - 2027/03/31

  5. Microbial evolution below the seafloor

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Japan Agency for Marine-Earth Science and Technology

    2022/04/01 - 2026/03/31

  6. Exploring the spatiotemporal distribution and interrelationships between hadal microbial ecosystems and turbidite systems in the Japan Trench

    Inagaki Fumio

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Japan Agency for Marine-Earth Science and Technology

    2022/04 - 2025/03

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    We analyzed bottom water and sediment samples collected from 15 sites along the Japan Trench by the International Ocean Discovery Program (IODP) Expedition 386 to estimate the diversity distribution and metabolic functions of hadal microbial communities using single-cell genomics and metagenomics techniques. As a result, we identified nine new phylogenetic groups in the phylum Atribacterota JS1, which is predominant in the Japan Trench samples, and found that their genomes contain genes encoding arginine-degrading enzymes such as clostripain, genes encoding ribose transporters, and genes related to glycine-betaine metabolisms. Furthermore, we detected tiny microplastic particles in those environments and characterized their chemical properties.

  7. Reconstruction of phytoplankton floral distribution before modern Ocean Warming

    Hagino Kyoko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Kochi University

    2020/04/01 - 2024/03/31

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    Global ocean warming has accelerated since the 1990s, resulting in the modification of marine phytoplankton habitats. However, the evidence of such an environmental impact on marine phytoplankton is limited. During the 1960s-70s, a huge collection of filter samples was obtained throughout the world's oceans by Profs. McIntyre of the Lamont-Doherty Earth Observatory and Prof. Okada of Hokkaido Univ.In this study, we assess the diversity of coccolithophores and parmales through morphological and eDNA metabarcoding studies using the filter samples. Our comprehensive survey employing morphological-molecular approaches provide important information on the phytoplankton community structure in the 1960s-70s, and enabled us to make a comparison with that found in the modern ocean. We also conducted culture and molecular phylogenetic studies of several coccolithophore species to be use the data for interpretation of the obtained metabarcoding data.

  8. Reconstruction of ancient ecosystems using sediment core environmental DNA

    HOSHINO Tatsuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Japan Agency for Marine-Earth Science and Technology

    2021/07 - 2024/03

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    DNA was extracted from sediments (up to 4 m deep) from six sites in Uranouchi Bay, Kochi Prefecture, Japan, which are coastal sediments, and amplicon sequencing of the 18S rRNA gene and COI gene was performed. The results showed that although many current organisms were detected in the surface layer, many DNAs from terrestrial plants were detected in sediments deeper than 150 cm, suggesting that these DNAs are likely to reflect past vegetation. Analysis of 18S rRNA in 300 marine sediments from around the world from deeper sediments (maximum depth 650 m below the seafloor) showed that eukaryotic DNA is widely preserved in sediments less than 100,000 years old. DNA from freshwater diatom species was also detected in sediments from glacial freshwater lakes in the Baltic Sea, suggesting that environmental DNA analysis can be used to reconstruct ancient ecosystems.

  9. 超低エネルギー環境における微生物の存続メカニズムの解明

    星野辰彦

    Offer Organization: 自然科学研究機構

    System: アストロバイオロジーセンター研究助成

    2022/04 - 2023/03

  10. Changes in the environments and biological communities revealed from the sediment core of Uranouchi Inlet Bay, Kochi during Anthropocene

    Murayama Masafumi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Kochi University

    2020/04 - 2023/03

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    The purpose of this study was to investigate the environmental changes in the Anthropocene from the sediments of Uranouchi inlet Bay, located in Kochi, Japan. Divers directly collected surface sediment cores from the inner (depth: 9.7m) and central bay (depth: 19.0m). The sediment consists of silty clay without any disturbance in sedimentary structures. Heavy metal elements (Cu, Zn, Ni, Cd, Cr) have shown a significant increase since around 1964 in the inner and 1950 in the central bay, currently exhibiting values nearly double at present. The total organic matter content has increased since around 1955, coinciding with the initiation of aquaculture in the region. Carbon and nitrogen isotope ratios have also undergone substantial changes, indicating alterations in their sources and the impact of nutrient input, such as fertilizers. The decrease in Mn content suggests a shift towards a more reduced bottom environment, occurring around 1977 in the inner and 1954 in the central bay.

  11. Construction of a model for the diffusion and circulation of deep subsurface life and carbon to the ocean via submarine mud volcanism

    Ijiri Akira

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Kobe University

    2020/04 - 2023/03

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    This study aims to reveal the interactions between the geosphere, hydrosphere, and biosphere through submarine mud volcano activity. During a research expedition conducted from December 2021 to January 2022, water and sediment samples were collected from the submarine mud volcanoes off Tanegashima Island. The origin of carbon substances and microbes released from the mud volcano was revealed through the analysis of dissolved organic matter, methane, and microbial community analysis. The behavior of these substances in the marine environment was investigated, and a quantitative analysis was performed to examine their relationship with mud volcano activity. Furthermore, the origin of the sediment and fluids erupted onto the seafloor due to the activity of the submarine mud volcanoes off Tanegashima Island was revealed through chemical analysis of the extracted water and gases from the mud volcano sediments.

  12. Elucidating genomic evolution and survival strategies of microbial communities inhabiting the oldest subterranean aquifer of the Earth

    Inagaki Fumio

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Pioneering)

    Institution: Japan Agency for Marine-Earth Science and Technology

    2020/04 - 2022/03

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    The world’s oldest subterranean aquifer has been discovered at the Kidd Creek mine in Ontario, Canada, which was estimated to be ~2.64 billion years old in the Proterozoic era. In this study, in collaboration with the University of Toronto, we conducted metagenomic and metatranscriptomic analyses of subterranean microbial communities. Microbial cells were collected by membrane filter units installed in a horizontal water extraction well at ~2.4 km below the land surface. The results suggest that metabolically active bacterial communities, predominantly belonging to the phylum Proteobacteria and Firmicutes in about 100 cells/mL, contribute to biogeochemical cycling, including alkane degradation and other redox reactions of hydrogen, carbon, sulfur, and arsenate compounds.

  13. 地球最古の地下水圏環境に生息する微生物群のゲノム進化と存続メカニズムの解明

    稲垣 史生, 鈴木 志野, 星野 辰彦, 浦本 豪一郎

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的研究(開拓)

    Category: 挑戦的研究(開拓)

    Institution: 国立研究開発法人海洋研究開発機構

    2019/06 - 2022/03

  14. Exploration of the limits and adaptations of life in subseafloor biosphere Competitive

    MORONO Yuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Japan Agency for Marine-Earth Science and Technology

    2019 - 2022

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    In this study, we investigated the life in subseafloor environments in the view of "nutrient supply (a critical requirement for sustaining life)" and "survival". The subseafloor sedimentary biosphere is characterized by gradual changes of parameters such as temperature, pressure, etc. In the deeper zones, both high temperature and pressure conditions restrict the survival of microbes. We investigated the profiles of microbial abundance and surrounding physicochemical parameters and found that even in extremely nutrient-poor environments, microbes can subsist and adapt to the surrounding environments.

  15. 地球最古の地下水圏環境における微生物生態系の存続と進化プロセスの解明 Competitive

    稲垣 史生

    Offer Organization: 日本学術振興会

    System: 国際共同研究強化B

    Category: 国際共同研究加速基金(国際共同研究強化(B))

    Institution: 国立研究開発法人海洋研究開発機構

    2019 - 2021

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    カナダ・オンタリオ州のキッド・クリーク鉱山の地下約2.5kmの岩体に設置された水平抜湯井から、生物サンプルを採取するため、農林水産省による輸入許可を得た。その後、カナダ・トロント大学のBarbara Sharwood Lollar教授のラボと共同で、10Lの地下水を採取し、DNA分析用と細胞計数・観察用にメンブレンフィルターを用いてろ過を行なった。それらのサンプルを適切に現地で処理し、ドライアイス詰めにして海洋研究開発機構高知コア研究所に発送した。鉱山内での作業時間や使用する機器・電源などに制限があり、水平抜湯井から湧出する地下水の流量が少なく、ガス成分に富んでいることから、採取した10Lの地下水では解析に十分な微生物細胞数を得られない可能性が考えられた。そのため、ろ過面積が大きくガス等による閉塞が少ない中空糸メンブレンフィルターのカートリッジを水平抜湯井にセットし、今後のサンプリングの機会に回収することとした。その後、高知コア研究所のラボにおいて、フィルターに捕獲された微生物細胞を蛍光染色し、当該環境に微生物細胞が存在することを確認した。予察的な検討では、地下水1 mLに含まれる細胞密度は約100細胞程度であることが示唆された。現在、メンブレンからDNAを抽出し、PCRで増幅された16S rRNA遺伝子の塩基配列に基づく微生物群集構造について解析を進めている。

  16. Evolution process of microbes in subseafloor Competitive

    HOSHINO Tatsuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Japan Agency for Marine-Earth Science and Technology

    2017 - 2020

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    Below the seafloor, which accounts for 70% of the Earth's surface, there is a unique ecosystem that is different from the surface biosphere, including ocean and soil, and a huge number of microorganisms have been confirmed to exist. In this study, we investigated how the ecosystem was constructed in the low energy subseafloor environment by analyzing sediments from 0-600 m below the seafloor. Microbial community structure in subseafloor differed significantly between anaerobic-eutrophic and aerobic-oligotrophic environments, with the archaea/bacteria ratio in the former being significantly higher than in the latter. The proportion of archaeal cells in the total sediment was estimated to be about 37%, comparable to that in the ocean.

  17. Exploration of subseafloor microbial biosphere through technical developments

    MORONO Yuki, ITO Motoo, HOSHINO Tatsuhiko, INAGAKI Fumio

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (A)

    Institution: Japan Agency for Marine-Earth Science and Technology

    2015/04/01 - 2018/03/31

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    In this study, we aimed to study microorganisms inhabiting subseafloor biosphere, which is one of the largest microbial biosphere on the Earth. Through developments of techniques to detect, separate, and selectively separate (sort) microbial cells with cleanest possible handling environment, we successfully detected very low biomass in the sediment and probe its metabolic activity through NanoSIMS (Nano-scale Secondary Ion Mass Spectroscopy) analysis.

  18. Development of procedure for single cell genomics of sedimentary microbial cells Competitive

    HOSHINO Tatsuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Japan Agency for Marine-Earth Science and Technology

    2015 - 2017

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    Single cell genomics is an effective tool for understanding microbial ecology in the natural environment because it can provide comprehensive information on microbial phylogeny and metabolic function. However, application of single cell genomics to microbial cells in subseafloor environment is hampered by the fact that DNA content in a single cells is very little to be amplified to enough amount for sequencing. In this study, we developed a procedure where proteinase K is used for lysis of cells and a metal plate is used for multiple displacement amplification of genomic DNA. As a result, we successfully amplified genomic DNA from sorted single cells isolated from subseafloor sediment.

  19. Census of Deep Subseafloor Life: Biogeographical distribution of microbial life in Earth's interior Competitive

    INAGAKI Fumio

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Japan Agency for Marine-Earth Science and Technology

    2014 - 2017

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    To elucidate biogeographical distribution of deep microbial life in the global subseafloor sedimentary environment which accounts for ~70% of the Earth's surface, we extracted environmental genomic DNA from frozen core samples obtained by multiple scientific ocean drilling expeditions in the past decade and performed the comprehensive quantification and sequence analysis of 16S rRNA genes. Consequently, the proportion of Archaea in the global subseafloor sedimentary biosphere was estimated at ~37%, which is nearly consistent with archaeal abundance in the overlying marine environment. In addition, analysis of microbial community structure and taxonomic composition demonstrated that the deep subseafloor biosphere can be categorized into two types: the aerobic microbial ecosystem in the open ocean and the anaerobic microbial ecosystem in the marginal ocean, which populations are persisting vertically into deeper sedimentary habitats.

  20. Estimation of subsidence records and earthquake damages of Nankai earthquakes from the investigation of structures and objects on the seafloor along the Kochi coastline.

    Tokuyama Hidekazu, MURAYAMA Masafumi, YAMAMOTO Yuji, HIROSE Takehiro, IJIRI Akira, HOSHINO Tatsuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Kochi University

    2014/04 - 2016/03

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    The fate of Kuroda-gori, a coastal village that was submerged under seawater due to subsidence after the Hakuho Earthquake in 684 AD, is widely known in Kochi Prefecture. In addition, structures presumed to be the remnants of historical villages have been observed on the seafloor along the Kochi coastline. This study was conducted to investigate the origin and characteristics of the sub-bottom structures to understand the nature of the land subsidence and elucidate the size of the tsunamis that occurred in Kochi region. We observed the structures at 5 sites on the seafloor near coastline. Our results suggest that structures at Tsumajiro and Nomi-bay areas are artificial, though the structures at Kashiwajima, To-chi, and off-Shiwa areas are probably natural. Stone pillars on the seafloor near Tsumajiro are probably carried by the flow of tsunami waves. Small terrace on the seafloor at Nomi bay area is habitable space and probably submerged due to subsidence after Nankai earthquakes.

  21. Quantification of the genes in global subseafloor sediments using a micro-fluidic digital PCR technique Competitive

    星野 辰彦

    Offer Organization: 米国スローン財団

    System: Deep Life Community pilot project

    2015 - 2015

  22. Phylogeny, function and physiology of microbes playing important roles in carbon flow in the marine subsurface environments Competitive

    HOSHINO Tatsuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    2012 - 2014

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    In the past decades, microbial community in the marine subsurface environments have been studied, and total biomass of microbial cells in the marine subsurface was estimated to contribute to 0.6% of the total living biomass carbon on the Earth. However, roles of those deep life were still largely unknown. In this study, we first investigated diversity of cooS gene, which encodes beta subunit of anaerobic carbon monoxide dehydrogenase, in the subseafloor environments. DNA was extracted from marine sediments obtained at Gulf of Mexico, Fuan de Fuca, South Pacific Gyre, Nankai Trough, and off Shimokita peninsula by IODP expeditions. Fragment of cooS gene was amplified by PCR and sequenced. The sequencing results indicated that diversity of cooS gene in the sediments were very small comparing to 16S rRNA gene. Almost all obtained sequences were not related to the known cooS gene sequences in the database and formed distinct clusters in a phylogenetic tree.

  23. 海底下生命圏に棲息する未知微生物の発見を目指した新規rRNA 配列解析手法の開発 Competitive

    星野 辰彦

    Offer Organization: 日本科学協会

    System: 笹川科学研究助成

    2012 - 2012

  24. シングルセルレベル解析が明らかにする海底下地下生命圏の微生物生態 Competitive

    星野 辰彦

    Offer Organization: 発酵研究所

    System: 発酵研究所一般研究助成

    2011 - 2012

  25. バイオフィルム内脱窒細菌の生態解明 -系統・機能・生理生態のin situ検出- Competitive

    星野 辰彦

    Offer Organization: 日本学術振興会

    System: 特別研究員奨励費（SPD）

    Category: 特別研究員奨励費

    2008 - 2009

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    平成20年度はin situ RCAのプロトコールを最適化し、P.stutzeri内部の脱窒に関わる遺伝子群(nirS,nosZ)を同時に検出する事に成功した。平成21年度はこのプロトコールを複合微生物生態系である環境サンプルに適用し、バクテリアの機能と系統をリンクすることを可能にする手法の開発をおこなった。サンプルとして、デンマーク・オールボーにある排水処理施設(生物学的リン除去プロセス)内の活性汚泥を用いた。活性汚泥から抽出したDNAからPCRにより脱窒を担う遺伝子の一つであるnirSを増幅し、クローンライブラリーを作成した。クローンライブラリー中で優占化するいくつかのクラスターを検出するためにin situ RCA用のプローブを設計し、活性汚泥中の脱窒細菌の検出を試みた結果、非常にクリアに脱窒細菌を検出できた。また、2種類のプローブを同時にアプライし、2種類の脱窒細菌の空間的分布を視覚化する事に成功した。さらに、検出された脱窒細菌の形態がリン蓄積細菌のものだと思われたため、16S rRNAを標的としたFISH法によりリン蓄積細菌を検出した後、in situ RCA法でnirS遺伝子を検出した。その結果、今回nirSクローンライブラリー中で優占化した配列がリン蓄積細菌由来であることがわかった。これは複合微生物系においてバクテリアの系統と機能をin situでリンクする事に成功した世界で初めての事例であり、機能と系統をリンクするという環境微生物生態学の10年来の課題を解決する成果である。今後、フローサイトメトリーやレーザーマイクロダイセクションなどにより、in situ RCAで機能に基づいて検出した細胞を分取、16S rRNA遺伝子の配列を決定することでさらに効率的に、機能と系統のリンクを行う事が可能であると思われる。

  26. Development of High-Performance Wastewater Treatment System Capable of Simultaneous Nitrogen and Phosphorus Removal by Controlling Microbial Ecology

    TSUNEDA Satoshi, AOI Yoshiteru, HOSHINO Tatsuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Waseda University

    2005 - 2008

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Teaching Experience 1

  1. 地球史　広島大学地球惑星システム学プログラム

Social Activities 11

  1. DNAワークショップ 海の生命を分子レベルから学ぶ

    東京都立西高等学校

    2026/01/31 - 2026/01/31

  2. ひらめき⭐︎ときめきサイエンス PCRを体験し、その原理を学ぼう

    高知みらい科学館

    2025/08/07 - 2025/08/07

  3. 海底のさらに下には何がいる？海底下生命圏の広がりと多様性

    海と地球の研究所〜海洋研究最前線！〜

    2024/12/08 - 2024/12/08

  4. ひらめきときめき⭐︎サイエンス PCRでDNAを増やし、その原理を学ぼう！

    高知みらい科学館

    2024/08/22 - 2024/08/22

  5. ペットボトルで顕微鏡を作ってみよう！

    「十川を知り、十川に学び、十川を愛する子」プロジェクト　四万十町立十川小学校

    2022/07/19 - 2022/07/19

  6. 顕微鏡をつくってみよう

    舟入小学校わくわく自然教室　香美市立舟入小学校

    2021/09/25 - 2021/09/25

  7. 深海のふしぎな生きものと海洋プラスチック汚染

    高新文化ホール

    2021/02/27 - 2021/02/27

  8. ペットボトル顕微鏡を使っていろいろなものを観察しよう

    高知子ども未来プロジェクト2020

    2020/10/30 - 2020/10/30

  9. 小さい微生物の見えない力

    高知大学附属中学校

    2020/01/31 - 2020/01/31

  10. 顕微鏡をつくってみよう

    高知大学附属中学校

    2019/12/05 - 2019/12/05

  11. 海底化に広がる巨大生命圏

    高知県立美術館ホール

    2019/11/07 - 2019/11/07

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