Details of the Researcher

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Tomonori Nochi
Section
Graduate School of Agricultural Science
Job title
Professor
Degree

  • 博士（農学）（東北大学）

  • 修士（農学）（東北大学）

e-Rad No.
10708001
Researcher ID
U-2533-2019
Profile

静岡県浜松市出身。１９歳で浜松を離れ、仙台、京都、東京、アメリカ（ノースカロライナ州）で生活した後、仙台に戻り、母校である東北大学で教育・研究活動に従事。研究テーマは、粘膜組織に発達する免疫臓器の形態形成、機能成熟に関する機構解明と家畜感染症予防・治療への応用。大学ならではの基礎研究を通した畜産業への貢献が目標。

Research History 18

  • 2025/04 - Present
    Tohoku University

  • 2025/04 - Present
    Tohoku University

  • 2024/10 - Present
    Tohoku University

  • 2023/04 - Present
    Tohoku University　Graduate School of Agricultural Science　Professor

  • 2022/11 - Present
    台北医学大学　栄養学院　客員教授

  • 2022/09 - Present
    University of Guelph　Ontario Agricultural College　Adjunct professor

  • 2022/06 - Present
    Tohoku University　Institute for Excellence in Higher Education　Professor

  • 2022/04 - Present
    The University of Tokyo

  • 2021/04 - Present
    Tohoku University Graduate School of Agricultural Science　Professor

  • 2020/04 - Present
    Tohoku University　Graduate School of Agricultural Science

  • 2023/04 - 2025/03
    東北大学高度教養教育・学生支援機構　大学教育支援センター　センター長

  • 2020/07 - 2022/10
    台北医学大学　客員准教授

  • 2015/04 - 2022/03
    The University of Tokyo　The Institute of Medical Science

  • 2013/04 - 2021/03
    Tohoku University　Graduate School of Agricultural Science

  • 2015/04 - 2020/03
    Tohoku University　Graduate School of Agricultural Science

  • 2009/07 - 2013/03
    The University of North Carolina at Chapel Hill

  • 2008/04 - 2011/03
    Japan Society for the Promotion of Science

  • 2005/04 - 2009/06
    The University of Tokyo　The Institute of Medical Science

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Education 3

  • Tohoku University　Graduate School of Agricultural Science

    2002/04 - 2005/03

  • Tohoku University　Graduate School of Agricultural Science

    2000/04 - 2002/03

  • Tohoku University　Faculty of Agriculture

    1996/04 - 2000/03

Committee Memberships 9

  • 日本無菌生物ノートバイオロジー学会　理事

    2025/01 - Present

  • 日本免疫学会　評議委員

    2024/01 - Present

  • 大学教育イノベーション日本　幹事

    2023/04 - Present

  • 日本乳房炎研究会　事務局長

    2019/10 - Present

  • 日本獣医学会　評議委員

    2013/10 - Present

  • 家畜感染症学会　事務局員

    2013/10 - Present

  • 東北畜産学会　幹事長

    2021/04 - 2023/03

  • 日本乳房炎研究会　評議委員

    2013/10 - 2019/09

  • 東北畜産学会　編集委員

    2015/04 - 2019/03

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Professional Memberships 13

  • 日本無菌生物ノートバイオロジー学会

  • Society for Mucosal Immunology

  • 東北畜産学会

  • 日本乳房炎研究会

  • 家畜感染症学会

  • 日本獣医学会

  • 日本畜産学会

  • 日本免疫学会

  • 大学教育学会

  • 日本産肉研究会

  • 動物用ワクチンーバイオ医薬品研究会

  • The American Association of Immunologists

  • 日本細菌学会

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Research Interests 4

  • 機能形態学

  • 腸管

  • 乳腺

  • Mucosal Immunology

Research Areas 2

  • Life sciences / Animal production science / 粘膜免疫学

  • Life sciences / Animal production science /

Awards 4

  1. 第３回高居百合子学術賞

    2015/10/09　日本乳房炎研究会

  2. 研究奨励賞

    2011　予防衛生協会

  3. 研究奨励賞

    2010　日本免疫学会

  4. 研究奨励賞

    2002　日本獣医解剖学会

Papers 109

  1. Freeze-dried fecal microorganisms as an effective biomaterial for the treatment of calves suffering from diarrhea Peer-reviewed

    Jahidul Islam, Natsuki Ohtani, Yu Shimizu, Masae Tanimizu, Yoshiaki Goto, Masumi Sato, Eiji Makino, Toru Shimada, Chise Ueda, Ayumi Matsuo, Yoshihisa Suyama, Yoshifumi Sakai, Niel A. Karrow, Hiroshi Yoneyama, Ryota Hirakawa, Mutsumi Furukawa, Hidekazu Tanaka, Tomonori Nochi

    Scientific Reports　14　(1)　2024/11/14

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-024-79267-5 　

    eISSN： 2045-2322

  2. Formation of the junctions between lymph follicles in the Peyer's patches even before postweaning activation Peer-reviewed

    Anri Teshigahara, Yuri Banba, Hiromi Yoshida, Mitsuji Kaji, Zhou Zhou, Nao Koyama, Yoshifumi Sakai, Niel A. Karrow, Kouetsu Ogasawara, Ryota Hirakawa, Jahidul Islam, Mutsumi Furukawa, Tomonori Nochi

    Scientific Reports　14　(1)　2024/07/09

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-024-65984-4 　

    eISSN： 2045-2322

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    Abstract Peyer’s patches (PPs), which contain an abundance of B and T cells, play a key role in inducing pivotal immune responses in the intestinal tract. PPs are defined as aggregated lymph follicles, which consist of multiple lymph follicles (LFs) that may interact with each other in a synergistic manner. LFs are thought to be spherical in shape; however, the characteristics of their structure are not fully understood. To elucidate changes in the structure of PPs as individuals grow, we generated serial 2D sections from entire PPs harvested from mice at 2, 4, and 10 weeks of age and performed a 3D analysis using a software, Amira. Although the number of LFs in PPs was not changed throughout the experiment, the volume and surface area of LFs increased significantly, indicating that LFs in PPs develop continuously by recruiting immune cells, even after weaning. In response to the dramatic changes in the intestinal environment after weaning, the development of germinal centers (GCs) in LFs was observed at 4 and 10 weeks (but not 2 weeks) of age. In addition, GCs gradually began to form away from the center of LFs and close to the muscle layer where export lymphatic vessels develop. Importantly, each LF was joined to the adjacent LF; this feature was observed even in preweaning nonactivated PPs. These results suggest that PPs may have a unique organization and structure that enhance immune functions, allowing cells in LFs to have free access to adjacent LFs and egress smoothly from PPs to the periphery upon stimulation after weaning.

  3. Development of a rational framework for the therapeutic efficacy of fecal microbiota transplantation for calf diarrhea treatment International-journal Peer-reviewed

    Jahidul Islam, Masae Tanimizu, Yu Shimizu, Yoshiaki Goto, Natsuki Ohtani, Kentaro Sugiyama, Eriko Tatezaki, Masumi Sato, Eiji Makino, Toru Shimada, Chise Ueda, Ayumi Matsuo, Yoshihisa Suyama, Yoshifumi Sakai, Mutsumi Furukawa, Katsuki Usami, Hiroshi Yoneyama, Hisashi Aso, Hidekazu Tanaka, Tomonori Nochi

    Microbiome　10　(1)　31-31　2022/02

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1186/s40168-021-01217-4 　

    eISSN： 2049-2618

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    <title>Abstract</title><sec> <title>Background</title> Establishing fecal microbiota transplantation (FMT) to prevent multifactorial diarrhea in calves is challenging because of the differences in farm management practices, the lack of optimal donors, and recipient selection. In this study, the underlying factors of successful and unsuccessful FMT treatment cases are elucidated, and the potential markers for predicting successful FMT are identified using fecal metagenomics via 16S rRNA gene sequencing, fecal metabolomics via capillary electrophoresis time-of-flight mass spectrometry, and machine learning approaches. </sec><sec> <title>Results</title> Specifically, 20 FMT treatment cases, in which feces from healthy donors were intrarectally transferred into recipient diarrheal calves, were conducted with a success rate of 70%. <italic>Selenomonas</italic> was identified as a microorganism genus that showed significant donor–recipient compatibility in successful FMT treatments. A strong positive correlation between the microbiome and metabolome data, which is a prerequisite factor for FMT success, was confirmed by Procrustes analysis in successful FMT (<italic>r</italic> = 0.7439, <italic>P</italic> = 0.0001). Additionally, weighted gene correlation network analysis confirmed the positively or negatively correlated pairs of bacterial taxa (family <italic>Veillonellaceae</italic>) and metabolomic features (i.e., amino acids and short-chain fatty acids) responsible for FMT success. Further analysis aimed at establishing criteria for donor selection identified the genus <italic>Sporobacter</italic> as a potential biomarker in successful donor selection. Low levels of metabolites, such as glycerol 3-phosphate, dihydroxyacetone phosphate, and isoamylamine, in the donor or recipients prior to FMT, are predicted to facilitate FMT. </sec><sec> <title>Conclusions</title> Overall, we provide the first substantial evidence of the factors related to FMT success or failure; these findings could improve the design of future microbial therapeutics for treating diarrhea in calves. </sec>

  4. The gut microbiota induces Peyer’s-patch-dependent secretion of maternal IgA into milk Peer-reviewed

    Katsuki Usami, Kanae Niimi, Ayumi Matsuo, Yoshihisa Suyama, Yoshifumi Sakai, Shintaro Sato, Kohtaro Fujihashi, Hiroshi Kiyono, Saeka Uchino, Mutsumi Furukawa, Jahidul Islam, Kaori Ito, Taiki Moriya, Yutaka Kusumoto, Michio Tomura, Russell C. Hovey, Junichi Sugawara, Hiroshi Yoneyama, Haruki Kitazawa, Kouichi Watanabe, Hisashi Aso, Tomonori Nochi

    Cell Reports　36　(10)　109655-109655　2021/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.celrep.2021.109655 　

    ISSN： 2211-1247

  5. Elucidation of the Effects of a Current X-SCID Therapy on Intestinal Lymphoid Organogenesis Using an In Vivo Animal Model. International-journal International-coauthorship Peer-reviewed

    Tomonori Nochi, Shunichi Suzuki, Shun Ito, Shotaro Morita, Mutsumi Furukawa, Daiichiro Fuchimoto, Yoji Sasahara, Katsuki Usami, Kanae Niimi, Osamu Itano, Minoru Kitago, Sachiko Matsuda, Ayumi Matsuo, Yoshihisa Suyama, Yoshifumi Sakai, Guoyao Wu, Fuller W Bazer, Kouichi Watanabe, Akira Onishi, Hisashi Aso

    Cellular and molecular gastroenterology and hepatology　10　(1)　83-100　2020/02/01

    DOI： 10.1016/j.jcmgh.2020.01.011 　

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    BACKGROUND & AIMS: Organ-level research using an animal model lacking Il2rg, the gene responsible for X-linked severe combined immunodeficiency (X-SCID), is clinically unavailable and would be a powerful tool to gain deeper insights into the symptoms of patients with X-SCID. METHODS: We used an X-SCID animal model, which was first established in our group by the deletion of Il2rg gene in pigs, to understand the clinical signs from multiple perspectives based on pathology, immunology, microbiology, and nutrition. We also treated the X-SCID pigs with bone marrow transplantation (BMT) for mimicking a current therapeutic treatment for patients with X-SCID and investigated the effect at the organ-level. Moreover, the results were confirmed using serum and fecal samples collected from patients with X-SCID. RESULTS: We demonstrated that X-SCID pigs completely lacked Peyer's patches (PPs) and IgA production in the small intestine, but possessed some dysfunctional intestinal T and B cells. Another novel discovery was that X-SCID pigs developed a heterogeneous intestinal microflora and possessed abnormal plasma metabolites, indicating that X-SCID could be an immune disorder that affects various in vivo functions. Importantly, the organogenesis of PPs in X-SCID pigs was not promoted by BMT. Although a few isolated lymphoid follicles developed in the small intestine of BMT-treated X-SCID pigs, there was no evidence that they contributed to IgA production and microflora formation. Consistently, most patients with X-SCID who received BMT possessed abnormal intestinal immune and microbial environments regardless of the presence of sufficient serum IgG. CONCLUSIONS: These results indicate that the current BMT therapies for patients with X-SCID may be insufficient to induce the organogenesis of intestinal lymphoid tissues that are associated with numerous functions in vivo.

  6. Development of immune and microbial environments is independently regulated in the mammary gland Peer-reviewed

    K. Niimi, K. Usami, Y. Fujita, M. Abe, M. Furukawa, Y. Suyama, Y. Sakai, M. Kamioka, N. Shibata, E. J. Park, S. Sato, H. Kiyono, H. Yoneyama, H. Kitazawa, K. Watanabe, T. Nochi, H. Aso

    Mucosal Immunology　11　(3)　643-653　2018/05/01

    Publisher: Nature Publishing Group

    DOI： 10.1038/mi.2017.90 　

    ISSN： 1935-3456 1933-0219

    eISSN： 1935-3456

  7. Cryptopatches Are Essential for the Development of Human GALT Peer-reviewed

    Tomonori Nochi, Paul W. Denton, Angela Wahl, J. Victor Garcia

    CELL REPORTS　3　(6)　1874-1884　2013/06

    DOI： 10.1016/j.celrep.2013.05.037 　

    ISSN： 2211-1247

  8. Nanogel antigenic protein-delivery system for adjuvant-free intranasal vaccines Peer-reviewed

    Tomonori Nochi, Yoshikazu Yuki, Haruko Takahashi, Shin-Ichi Sawada, Mio Mejima, Tomoko Kohda, Norihiro Harada, Il Gyu Kong, Ayuko Sato, Nobuhiro Kataoka, Daisuke Tokuhara, Shiho Kurokawa, Yuko Takahashi, Hideo Tsukada, Shunji Kozaki, Kazunari Akiyoshi, Hiroshi Kiyono

    Nature Materials　9　(7)　572-578　2010

    Publisher: Nature Publishing Group

    DOI： 10.1038/nmat2784 　

    ISSN： 1476-4660 1476-1122

    eISSN： 1476-4660

  9. Uptake through glycoprotein 2 of FimH+ bacteria by M cells initiates mucosal immune response Peer-reviewed

    Koji Hase, Kazuya Kawano, Tomonori Nochi, Gemilson Soares Pontes, Shinji Fukuda, Masashi Ebisawa, Kazunori Kadokura, Toru Tobe, Yumiko Fujimura, Sayaka Kawano, Atsuko Yabashi, Satoshi Waguri, Gaku Nakato, Shunsuke Kimura, Takaya Murakami, Mitsutoshi Iimura, Kimiyo Hamura, Shin-Ichi Fukuoka, Anson W. Lowe, Kikuji Itoh, Hiroshi Kiyono, Hiroshi Ohno

    Nature　462　(7270)　226-230　2009/11/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/nature08529 　

    ISSN： 0028-0836 1476-4687

    eISSN： 1476-4687

  10. A novel M cell-specific carbohydrate-targeted mucosal vaccine effectively induces antigen-specific immune responses Peer-reviewed

    Tomonori Nochi, Yoshikazu Yuki, Akiko Matsumura, Mio Mejima, Kazutaka Terahara, Dong-Young Kim, Satoshi Fukuyama, Kiyoko Iwatsuki-Horimoto, Yoshihiro Kawaoka, Tomoko Kohda, Shunji Kozaki, Osamu Igarashi, Hiroshi Kiyono

    Journal of Experimental Medicine　204　(12)　2789-2796　2007/11/26

    Publisher: Rockefeller University Press

    DOI： 10.1084/jem.20070607 　

    ISSN： 0022-1007

    eISSN： 1540-9538

  11. Rice-based mucosal vaccine as a global strategy for cold-chain- and needle-free vaccination Peer-reviewed

    Tomonori Nochi, Hidenori Takagi, Yoshikazu Yuki, Lijun Yang, Takehiro Masumura, Mio Mejima, Ushio Nakanishi, Akiko Matsumura, Akihiro Uozumi, Takachika Hiroi, Shigeto Morita, Kunisuke Tanaka, Fumio Takaiwa, Hiroshi Kiyono

    Proceedings of the National Academy of Sciences of the United States of America　104　(26)　10986-10991　2007/06/26

    Publisher: Proceedings of the National Academy of Sciences

    DOI： 10.1073/pnas.0703766104 　

    ISSN： 0027-8424

    eISSN： 1091-6490

  12. Development of prediction equations for immunoglobulin A, immunoglobulin G, and immunoglobulin M concentrations in mature milk from Holstein cows using milk infrared spectral data Peer-reviewed

    Yuri Satake, Teppei Katsura, Tao Zhuang, Megumi Urakawa, Yugo Mineshima, Toshimi Baba, Gaku Yoshida, Haruki Kitazawa, Hitoshi Shirakawa, Takehiko Nakamura, Tomonori Nochi, Yoshifumi Sakai, Masahiro Satoh, Satoshi Haga, Hisashi Aso, Yoshinobu Uemoto

    Journal of Dairy Science　108　(7)　7354-7369　2025/07

    Publisher: American Dairy Science Association

    DOI： 10.3168/jds.2024-25991 　

    ISSN： 0022-0302

  13. Milk sialyl-oligosaccharides mediate the early colonization of gut commensal microbes in piglets Peer-reviewed

    Ryoga Hashimoto, Keita Nishiyama, Fu Namai, Kasumi Suzuki, Taiga Sakuma, Itsuko Fukuda, Yuta Sugiyama, Kenji Okano, Takafumi Shanoh, Eita Toyoshi, Ryusuke Ohgi, Sudeb Saha, Sae Tsuchida, Eri Nishiyama, Takao Mukai, Mutsumi Furukawa, Tomonori Nochi, Julio Villena, Wakako Ikeda-Ohtsubo, Gou Yoshioka, Eri Nakazaki, Yoshihito Suda, Haruki Kitazawa

    Microbiome　13　(1)　2025/05/24

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1186/s40168-025-02129-3 　

    eISSN： 2049-2618

  14. Breast milk stabilizes bacterial communities in the large intestine even after weaning Peer-reviewed

    Kaori Ito, Jahidul Islam, Kota Sakurai, Saeka Koyama, Ayumi Matsuo, Kunihiro Okano, Ryota Hirakawa, Mutsumi Furukawa, Tomonori Nochi

    Biochemical and Biophysical Research Communications　151585-151585　2025/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.bbrc.2025.151585 　

    ISSN： 0006-291X

  15. Elevated levels of cyclophilin A secreted in milk during bovine mastitis

    Satoru Takanashi, Kozue Yoshimura, Yuma Endo, Megumi Urakawa, Hidetoshi Sato, Tao Zhuang, Tomohito Hayashi, Yoshio Kiku, Yuya Nagasawa, Haruki Kitazawa, Michael T. Rose, Yoshinobu Uemoto, Kouichi Watanabe, Tomonori Nochi, Hisashi Aso

    Journal of Dairy Science　2024/10

    Publisher: American Dairy Science Association

    DOI： 10.3168/jds.2024-24715 　

    ISSN： 0022-0302

  16. Male mice are susceptible to brain dysfunction induced by early-life acephate exposure Peer-reviewed

    Takahiro Sasaki, Jahidul Islam, Kenshiro Hara, Tomonori Nochi, Kentaro Tanemura

    Frontiers in Neuroscience　18　2024/07/10

    Publisher: Frontiers Media SA

    DOI： 10.3389/fnins.2024.1404009 　

    eISSN： 1662-453X

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    Background Acephate is a widely used organophosphate insecticide. Exposure to endocrine-disrupting chemicals, such as acephate, can interfere with neurodevelopment in childhood, increasing the risk of higher brain dysfunction later in life. Furthermore, brain dysfunction may be related to chemical exposure-related disturbances in the gut microbiota. However, the effects of early acephate exposure on the brains of adult males and females as well as on the adult gut environment remain poorly understood. Methods This study investigated the effects of perinatal acephate exposure on the central nervous system and gut microbiota of mice, including sex differences and environmentally relevant concentrations. C57BL/6 N pups were exposed to acephate (0, 0.3, 10, and 300 ppm) via the dam in their drinking water from embryonic day (E) 11.5 to postnatal day 14. We examined its effects on the central nervous system of adult males and females. Results In the male treatment group, impairments in learning and memory were detected. Immunohistochemical analysis revealed a decrease in SOX2-, NeuN-, DCX-, and GFAP-positive cells in the hippocampal dentate gyrus in males compared to the control group, whereas GFAP-positive cells were fewer in females. In addition, gut microbiota diversity was reduced in both sexes in the experimental group. Conclusion Our study demonstrates that the effects of early-life exposure to acephate are more pronounced in males than in females and can lead to a lasting impact on adult behavior, even at low doses, and that the gut microbiota may reflect the brain environment.

  17. The impact of high-IgE levels on metabolome and microbiome in experimental allergic enteritis. International-journal Peer-reviewed

    Elisa Zubeldia-Varela, Frank Blanco-Pérez, Tomás Clive Barker-Tejeda, David Rojo, Alma Villaseñor, Jahidul Islam, Irene Gonzalez-Menendez, Jonathan Laiño, Maren Krause, Hanna Steigerwald, Manuela Martella, Leticia Quintanilla-Martinez, Philipp Yu, Coral Barbas, Stefan Vieths, Tomonori Nochi, Domingo Barber, Masako Toda, Marina Pérez-Gordo

    Allergy　2024/06/23

    DOI： 10.1111/all.16202 　

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    BACKGROUND: The pathological mechanism of the gastrointestinal forms of food allergies is less understood in comparison to other clinical phenotypes, such as asthma and anaphylaxis Importantly, high-IgE levels are a poor prognostic factor in gastrointestinal allergies. METHODS: This study investigated how high-IgE levels influence the development of intestinal inflammation and the metabolome in allergic enteritis (AE), using IgE knock-in (IgEki) mice expressing high levels of IgE. In addition, correlation of the altered metabolome with gut microbiome was analysed. RESULTS: Ovalbumin-sensitized and egg-white diet-fed (OVA/EW) BALB/c WT mice developed moderate AE, whereas OVA/EW IgEki mice induced more aggravated intestinal inflammation with enhanced eosinophil accumulation. Untargeted metabolomics detected the increased levels of N-tau-methylhistamine and 2,3-butanediol, and reduced levels of butyric acid in faeces and/or sera of OVA/EW IgEki mice, which was accompanied with reduced Clostridium and increased Lactobacillus at the genus level. Non-sensitized and egg-white diet-fed (NC/EW) WT mice did not exhibit any signs of AE, whereas NC/EW IgEki mice developed marginal degrees of AE. Compared to NC/EW WT mice, enhanced levels of lysophospholipids, sphinganine and sphingosine were detected in serum and faecal samples of NC/EW IgEki mice. In addition, several associations of altered metabolome with gut microbiome-for example Akkermansia with lysophosphatidylserine-were detected. CONCLUSIONS: Our results suggest that high-IgE levels alter intestinal and systemic levels of endogenous and microbiota-associated metabolites in experimental AE. This study contributes to deepening the knowledge of molecular mechanisms for the development of AE and provides clues to advance diagnostic and therapeutic strategies of allergic diseases.

  18. Broad specificity of monoclonal IgA (TEPC15-IgA) for enteric bacteria via phosphorylcholine-mediated interaction. Peer-reviewed

    Saeka Koyama, Kaori Ito, Katsuki Usami, Shino Wada, Tsukasa Yamashita, Wakako Ikeda-Ohtsubo, Haruki Kitazawa, Ryota Hirakawa, Jahidul Islam, Mutsumi Furukawa, Tomonori Nochi

    The Journal of veterinary medical science　2024/06/04

    DOI： 10.1292/jvms.23-0441 　

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    Immunoglobulin A (IgA) is notable for its broad specificity toward multiple bacteria. Phosphorylcholine (PC) plays a role in the infection of pathogenic bacteria carrying PC and in the induction of IgA responses in the host immune system. The commercially available mouse monoclonal IgA, TEPC15-IgA, is a distinctive antibody with specificity for PC, warranting further exploration of its response to PC-bearing enteric bacteria. In this study, using 17 different enteric bacteria, including 3 aerobic and 14 anerobic bacteria that could be cultured in vitro, we confirmed that TEPC15-IgA recognizes 4 bacterial species: Lactobacillus taiwanensis, Limosilactobacillus frumenti, Streptococcus infantis, and Escherichia coli, although reactivity varied. Interestingly, TEPC15-IgA did not react with four of six Lactobacillus species used. Moreover, distinct target molecules associated with PC in L. taiwanensis and L. frumenti were evident, differing in molecular weight. These findings suggest that the natural generation of PC-specific IgA could prevent PC-mediated infections and potentially facilitate the formation of a microflora rich in indigenous bacteria with PC, particularly in the gastrointestinal tract.

  19. Identification of four genes responsible for antimicrobial resistance of MEL-B against S. aureus Peer-reviewed

    Shinya Yamauchi, So Shimoda, Akio Kawahara, Tomohiro Sugahara, Shuhei Yamamoto, Masao Kitabayashi, Atsushi Sogabe, Christine A. Jansen, Ryuta Tobe, Ryota Hirakawa, Jahidul Islam, Mutsumi Furukawa, Hiroshi Yoneyama, Tomonori Nochi

    Biochemical and Biophysical Research Communications　149566-149566　2024/01

    Publisher: Elsevier BV

    DOI： 10.1016/j.bbrc.2024.149566 　

    ISSN： 0006-291X

  20. Combined Lycium barbarum Polysaccharides with Plasmon-Activated Water Affect IFN-γ/TNF-α Induced Inflammation in Caco-2 Cells Peer-reviewed

    Yu Zhi Lian, Yu-Chuan Liu, Chun-Chao Chang, Tomonori Nochi, Jane C.-J. Chao

    Pharmaceuticals　16　(10)　1455-1455　2023/10/13

    Publisher: MDPI AG

    DOI： 10.3390/ph16101455 　

    eISSN： 1424-8247

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    The effects of Lycium barbarum polysaccharides (LBP) and plasmon-activated water (PAW) against IFN-γ/TNF-α induced inflammation in human colon Caco-2 cells were investigated. Cells were divided into the control, induction, LBP treatment (100–500 μg/mL), and combination groups with PAW. Inflammation was induced 24 h with 10 ng/mL IFN-γ when cell confluency reached &gt;90%, and various doses of LBP with or without PAW were treated for 3 h, and subsequently 50 ng/mL TNF-α was added for another 24 h to provoke inflammation. Combination of LBP with PAW significantly decreased the secretion of IL-6 and IL-8. Cyclooxygenase-2 and inducible NO synthase expression was attenuated in all LBP-treated groups with or without PAW. NLRP3 inflammasome and related protein PYCARD expression were inhibited by LBP at the highest dose (500 μg/mL). All doses of LBP alone significantly decreased p-ERK expression, but combination with PAW increased p-ERK expression compared to those without PAW. Additionally, 250 and 500 μg/mL of LBP with or without PAW inhibited procaspase-3/caspase-3 expression. Therefore, LBP possesses anti-inflammation and anti-apoptosis by inhibiting the secretion of inflammatory cytokines and the expression of NLRP3 inflammasome-related protein. The combination with PAW exerts additive or synergistic effect on anti-inflammation.

  21. Genetic aspects of immunoglobulins and cyclophilin A in milk as potential indicators of mastitis resistance in Holstein cows Peer-reviewed

    Yoshinobu Uemoto, Teppei Katsura, Yuma Endo, Koutaro Tanaka, Tao Zhuang, Megumi Urakawa, Toshimi Baba, Gaku Yoshida, Haifei Wang, Haruki Kitazawa, Hitoshi Shirakawa, Takehiko Nakamura, Tomonori Nochi, Hisashi Aso

    Journal of Dairy Science　2023/10

    Publisher: American Dairy Science Association

    DOI： 10.3168/jds.2022-23075 　

    ISSN： 0022-0302

  22. Protective Effects of Gnetin C from Melinjo Seed Extract against High-Fat Diet-Induced Hepatic Steatosis and Liver Fibrosis in NAFLD Mice Model Peer-reviewed

    Tohfa Kabir, Haruki Yoshiba, Afifah Zahra Agista, Halima Sultana, Yusuke Ohsaki, Chiu-Li Yeh, Ryota Hirakawa, Hiroko Tani, Tomoki Ikuta, Tomonori Nochi, Suh-Ching Yang, Hitoshi Shirakawa

    Nutrients　15　(18)　3888-3888　2023/09/06

    Publisher: MDPI AG

    DOI： 10.3390/nu15183888 　

    eISSN： 2072-6643

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    Nonalcoholic fatty liver disease (NAFLD), the most common form of chronic liver disease, can progress to hepatic steatosis, inflammation, and advanced fibrosis, increasing the risk of cirrhosis. Resveratrol, a natural polyphenol with antioxidant and anti-inflammatory properties, is beneficial in treating multiple metabolic diseases. Gnetin C, a resveratrol derivative obtained from Melinjo seed extract (MSE), shares similar health-promoting properties. We investigated the role of gnetin C in preventing NAFLD in a mouse model and compared it with resveratrol. Male C57BL/6J mice were fed a control diet (10% calories from fat), a high-fat choline-deficient (HFCD) diet (46% calories from fat) and HFCD diet supplemented with gnetin C (150 mg/kg BW·day−1) or resveratrol (150 mg/kg BW·day−1) for 12 weeks. Gnetin C supplementation reduced body and liver weight, and improved blood glucose levels and insulin sensitivity. Both gnetin C- and resveratrol reduced hepatic steatosis, with gnetin C also decreasing liver lipid content. Gnetin C and resveratrol ameliorated HFCD diet-induced hepatic fibrosis. The mRNA expression results, and western blot analyses showed that gnetin C and, to some extent, resveratrol downregulated fibrosis markers in the TGF-β1 signaling pathway, indicating a possible safeguarding mechanism against NAFLD. These results suggest that gnetin C supplementation may protect against lipid deposition and hepatic fibrosis.

  23. β‐1,3‐glucan, but not β‐1,3/1,6‐glucan, exacerbates experimental food allergy, while both increase <scp>IgA</scp> induction International-journal Peer-reviewed

    Chaoqi He, Yunhui Liu, Stefan Schülke, Shunsuke Nishio, Yingnan Guo, Hannah Rainer, Krause Maren, Ting‐Yu Cheng, Tomonori Nochi, Stefan Vieths, Stephan Scheurer, Tsukasa Matsuda, Masako Toda

    Allergy　79　(2)　503-506　2023/08/11

    Publisher: Wiley

    DOI： 10.1111/all.15841 　

    ISSN： 0105-4538

    eISSN： 1398-9995

  24. Effects of early-life tosufloxacin tosilate hydrate administration on growth rate, neurobehavior, and gut microbiota at adulthood in male mice. Peer-reviewed

    Ayano Hasegawa, Takahiro Sasaki, Jahidul Islam, Takashi Tominaga, Tomonori Nochi, Kenshiro Hara, Kentaro Tanemura

    The Journal of toxicological sciences　48　(3)　149-159　2023

    DOI： 10.2131/jts.48.149 　

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    Reportedly, antibiotics, which are frequently prescribed in children, have long-term effects owing to gut microbiota dysregulation. Tosufloxacin tosilate hydrate (TFLX) is the first orally administered new quinolone with high efficacy and broad-spectrum action approved as an antibacterial agent for pediatric use in Japan. However, studies on the effects of its early-stage administration are limited. Therefore, we aimed to analyze the later effects of its developmental administration by monitoring growth rate, neurobehavior, and gut microbiota in mice. The TFLX was administered via drinking water at a dose of up to 300 mg/kg for two consecutive weeks during the developmental period (4-6 weeks of age) or adulthood (8-10 weeks of age). Thereafter, the body weights of the mice were measured weekly to monitor growth rate. Behavioral tests were also conducted on 11-12-week-old mice to examine the neurobehavioral effects of the treatment. Further, to examine the effects of the treatment on microbiota, fecal samples were collected from the rectum of mice dissected at 12 weeks of age, and 16s rRNA analysis was conducted. Our results showed increased body weights after TFLX administration, without any long-term effects. Behavioral analysis suggested alterations in anxiety-like behaviors and memory recall dysregulation, and gut microbiota analysis revealed significant differences in bacterial composition. These findings indicated that TFLX administration during the developmental period affects mice growth rate, neurobehavior, and gut microbiota structure. This is the first study to report that TFLX is potentially associated with the risk of long effects.

  25. Roles of mannosylerythritol lipid-B components in antimicrobial activity against bovine mastitis-causing Staphylococcus aureus International-journal Peer-reviewed

    Shinya Yamauchi, Mutsumi Furukawa, Akio Kawahara, Tomohiro Sugahara, Shuhei Yamamoto, Masao Kitabayashi, Atsushi Sogabe, So Shimoda, Eiji Hata, Kouichi Watanabe, Hiroshi Yoneyama, Hisashi Aso, Tomonori Nochi

    World Journal of Microbiology and Biotechnology　38　(3)　54-54　2022/03

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s11274-022-03243-2 　

    ISSN： 0959-3993

    eISSN： 1573-0972

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    <title>Abstract</title>Mannosylerythritol lipid-B (MEL-B), which comprises ester-bonded hydrophilic ME and hydrophobic fatty acids, is a bio-surfactant with various unique properties, including antimicrobial activity against most gram-positive bacteria. The gram-positive <italic>Staphylococcus aureus</italic> is a causative pathogen of dairy cattle mastitis, which results in considerable economic loss in the dairy industry. Here, we demonstrate the efficacy of MEL-B as a disinfectant against bovine-derived <italic>S. aureus</italic> and elucidate a mechanism of action of MEL-B in the inhibition of bacterial growth. The growth of bovine mastitis causative <italic>S. aureus</italic> BM1006 was inhibited when cultured with MEL-B above 10 ppm. The activity of MEL-B required fatty acids (i.e., caprylic and myristoleic acids) as ME, the component of MEL-B lacking fatty acids, did not inhibit the growth of <italic>S. aureus</italic> even at high concentrations. Importantly, ME-bound fatty acids effectively inhibited the growth of <italic>S. aureus</italic> when compared with free fatty acids. Specifically, the concentrations of ME-bound fatty acids and free caprylic and myristoleic acids required to inhibit the growth of <italic>S. aureus</italic> were 10, 1442, and 226 ppm, respectively. The involvement of ME in the antimicrobial activity of MEL-B was confirmed by digestion of MEL-B with alkali, which dissociated ME and fatty acids. These results indicated that a mechanism of action of MEL-B in inhibiting the growth of <italic>S. aureus</italic> could be explained by the effective transporting of antimicrobial fatty acids to the bacterial surface via hydrophilic ME.

  26. Prevention of mastitis in multiparous dairy cows with a previous history of mastitis by oral feeding with probiotic <scp> Bacillus subtilis </scp>

    Megumi Urakawa, Tao Zhuang, Hidetoshi Sato, Satoru Takanashi, Kozue Yoshimura, Yuma Endo, Teppei Katsura, Tsuyoshi Umino, Koutaro Tanaka, Hitoshi Watanabe, Hiroko Kobayashi, Naokazu Takada, Tomoyuki Kozutsumi, Hiroaki Kumagai, Takafumi Asano, Kohko Sazawa, Nobuhisa Ashida, Guoqi Zhao, Michael T. Rose, Haruki Kitazawa, Hitoshi Shirakawa, Kouichi Watanabe, Tomonori Nochi, Takehiko Nakamura, Hisashi Aso

    Animal Science Journal　93　(1)　2022/01

    Publisher: Wiley

    DOI： 10.1111/asj.13764 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  27. Effects of vitamin E supplementation on serum oxidative stress biomarkers, antibody titer after live bovine respiratory syncytial virus vaccination, as well as serum and fecal immunoglobulin A in weaned Japanese Black calves

    Konosuke OTOMARU, Takuro MIYAHARA, Hiroto SAITA, Shinya YAMAUCHI, Tomonori NOCHI

    Journal of Veterinary Medical Science　84　(8)　1128-1133　2022

    Publisher: Japanese Society of Veterinary Science

    DOI： 10.1292/jvms.22-0170 　

    ISSN： 0916-7250

    eISSN： 1347-7439

  28. Differential expression of CD11c defines two types of tissue-resident macrophages with different origins in steady-state salivary glands Peer-reviewed

    Lu Lu, Toshinobu Kuroishi, Yukinori Tanaka, Mutsumi Furukawa, Tomonori Nochi, Shunji Sugawara

    Scientific Reports　12　(1)　2022/01

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-022-04941-5 　

    eISSN： 2045-2322

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    <title>Abstract</title>Gland macrophages are primed for gland development and functions through interactions within their niche. However, the phenotype, ontogeny, and function of steady-state salivary gland (SG) macrophages remain unclear. We herein identified CD11c+ and CD11c− subsets among CD64+ macrophages in steady-state murine SGs. CD11c− macrophages were predominant in the SGs of embryonic and newborn mice and decreased with advancing age. CD11c+ macrophages were rarely detected in the embryonic period, but rapidly expanded after birth. CD11c+, but not CD11c−, macrophage numbers decreased in mice treated with a CCR2 antagonist, suggesting that CD11c+ macrophages accumulate from bone marrow-derived progenitors in a CCR2-dependent manner, whereas CD11c− macrophages were derived from embryonic progenitors in SGs. CD11c+ and CD11c− macrophages strongly expressed colony-stimulating factor (CSF)-1 receptor, the injection of an anti-CSF-1 receptor blocking antibody markedly reduced both subsets, and SGs strongly expressed CSF-1, indicating the dependency of SG resident macrophage development on CSF-1. The phagocytic activity of SG macrophages was extremely weak; however, the gene expression profile of SG macrophages indicated that SG macrophages regulate gland development and functions in SGs. These results suggest that SG CD11c+ and CD11c− macrophages are developed and instructed to perform SG-specific functions in steady-state SGs.

  29. Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows International-journal

    Yutaka Suzuki, Sachi Chiba, Koki Nishihara, Keiichi Nakajima, Akihiko Hagino, Won-Seob Kim, Hong-Gu Lee, Tomonori Nochi, Toru Suzuki, Sang-Gun Roh

    Animals　11　(11)　3194-3194　2021/11/09

    Publisher: MDPI AG

    DOI： 10.3390/ani11113194 　

    eISSN： 2076-2615

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    Epithelial barrier function in the mammary gland acts as a forefront of the defense mechanism against mastitis, which is widespread and a major disorder in dairy production. Chemerin is a chemoattractant protein with potent antimicrobial ability, but its role in the mammary gland remains unelucidated. The aim of this study was to determine the function of chemerin in mammary epithelial tissue of dairy cows in lactation or dry-off periods. Mammary epithelial cells produced chemerin protein, and secreted chemerin was detected in milk samples. Chemerin treatment promoted the proliferation of cultured bovine mammary epithelial cells and protected the integrity of the epithelial cell layer from hydrogen peroxide (H2O2)-induced damage. Meanwhile, chemerin levels were higher in mammary tissue with mastitis. Tumor necrosis factor alpha (TNF-α) strongly upregulated the expression of the chemerin-coding gene (RARRES2) in mammary epithelial cells. Therefore, chemerin was suggested to support mammary epithelial cell growth and epithelial barrier function and to be regulated by inflammatory stimuli. Our results may indicate chemerin as a novel therapeutic target for diseases in the bovine mammary gland.

  30. Fermented rice bran supplementation attenuates chronic colitis-associated extraintestinal manifestations in female C57BL/6N mice International-journal

    Jahidul Islam, Afifah Zahra Agista, Kouichi Watanabe, Tomonori Nochi, Hisashi Aso, Yusuke Ohsaki, Takuya Koseki, Michio Komai, Hitoshi Shirakawa

    The Journal of Nutritional Biochemistry　99　108855-108855　2021/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.jnutbio.2021.108855 　

    ISSN： 0955-2863

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    Patients with inflammatory bowel disease (IBD) have higher incidence of extraintestinal manifestations (EIM), including liver disorders, sarcopenia, and neuroinflammation. Fermented rice bran (FRB), generated from rice bran (RB), is rich in bioactive compounds, and exhibits anti-colitis activity. However, its role in EIM prevention is still unclear. Here, for the first time, we investigated whether EIM in female C57Bl/6N mice is attenuated by FRB supplementation. EIM was induced by repeated administration of 1.5% dextran sulfate sodium (DSS) in drinking water (4 d) followed by drinking water (12 d). Mice were divided into 3 groups-control (AIN93M), 10% RB, and 10% FRB. FRB ameliorated relapsing colitis and inflammation in muscle by significantly lowering proinflammatory cytokines Tnf-α and Il-6 in serum and advanced glycation end product-specific receptor (Ager) in serum and muscle when compared with the RB and control groups. As FRB reduced aspartate aminotransferase levels and oxidative stress, it might prevent liver disorders. FRB downregulated proinflammatory cytokine and chemokine transcripts responsible for neuroinflammation in the hippocampus and upregulated mRNA expression of G protein coupled receptors (GPRs), Gpr41 and Gpr43, in small and large intestines, which may explain the FRB-mediated protective mechanism. Hence, FRB can be used as a supplement to prevent IBD-associated EIM.

  31. Ligilactobacillus salivarius Strains Isolated From the Porcine Gut Modulate Innate Immune Responses in Epithelial Cells and Improve Protection Against Intestinal Viral-Bacterial Superinfection International-journal

    Yuhki Indo, Shugo Kitahara, Mikado Tomokiyo, Shota Araki, Md. Aminul Islam, Binghui Zhou, Leonardo Albarracin, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Takato Takenouchi, Hirohide Uenishi, Hisashi Aso, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa

    Frontiers in Immunology　12　652923-652923　2021/06/07

    Publisher: Frontiers Media SA

    DOI： 10.3389/fimmu.2021.652923 　

    eISSN： 1664-3224

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    Previously, we constructed a library of <italic>Ligilactobacillus salivarius</italic> strains from the intestine of wakame-fed pigs and reported a strain-dependent capacity to modulate IFN-β expression in porcine intestinal epithelial (PIE) cells. In this work, we further characterized the immunomodulatory activities of <italic>L. salivarius</italic> strains from wakame-fed pigs by evaluating their ability to modulate TLR3- and TLR4-mediated innate immune responses in PIE cells. Two strains with a remarkable immunomodulatory potential were selected: <italic>L. salivarius</italic> FFIG35 and FFIG58. Both strains improved IFN-β, IFN-λ and antiviral factors expression in PIE cells after TLR3 activation, which correlated with an enhanced resistance to rotavirus infection. Moreover, a model of enterotoxigenic <italic>E. coli</italic> (ETEC)/rotavirus superinfection in PIE cells was developed. Cells were more susceptible to rotavirus infection when the challenge occurred in conjunction with ETEC compared to the virus alone. However, <italic>L. salivarius</italic> FFIG35 and FFIG58 maintained their ability to enhance IFN-β, IFN-λ and antiviral factors expression in PIE cells, and to reduce rotavirus replication in the context of superinfection. We also demonstrated that FFIG35 and FFIG58 strains regulated the immune response of PIE cells to rotavirus challenge or ETEC/rotavirus superinfection through the modulation of negative regulators of the TLR signaling pathway. <italic>In vivo</italic> studies performed in mice models confirmed the ability of <italic>L. salivarius</italic> FFIG58 to beneficially modulate the innate immune response and protect against ETEC infection. The results of this work contribute to the understanding of beneficial lactobacilli interactions with epithelial cells and allow us to hypothesize that the FFIG35 or FFIG58 strains could be used for the development of highly efficient functional feed to improve immune health status and reduce the severity of intestinal infections and superinfections in weaned piglets.

  32. Fermented Rice Bran Supplementation Prevents the Development of Intestinal Fibrosis Due to DSS-Induced Inflammation in Mice

    Afifah Zahra Agista, Tubagus Bahtiar Rusbana, Jahidul Islam, Yusuke Ohsaki, Halima Sultana, Ryota Hirakawa, Kouichi Watanabe, Tomonori Nochi, Ardiansyah, Slamet Budijanto, Suh-Ching Yang, Takuya Koseki, Hisashi Aso, Michio Komai, Hitoshi Shirakawa

    Nutrients　13　(6)　1869-1869　2021/05/30

    Publisher: MDPI AG

    DOI： 10.3390/nu13061869 　

    eISSN： 2072-6643

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    Fermented rice bran (FRB) is known to protect mice intestines against dextran sodium sulfate (DSS)-induced inflammation; however, the restoration of post-colitis intestinal homeostasis using FRB supplementation is currently undocumented. In this study, we observed the effects of dietary FRB supplementation on intestinal restoration and the development of fibrosis after DSS-induced colitis. DSS (1.5%) was introduced in the drinking water of mice for 5 days. Eight mice were sacrificed immediately after the DSS treatment ended. The remaining mice were divided into three groups, comprising the following diets: control, 10% rice bran (RB), and 10% FRB-supplemented. Diet treatment was continued for 2 weeks, after which half the population of mice from each group was sacrificed. The experiment was continued for another 3 weeks before the remaining mice were sacrificed. FRB supplementation could reduce the general observation of colitis and production of intestinal pro-inflammatory cytokines. FRB also increased intestinal mRNA levels of anti-inflammatory cytokine, tight junction, and anti-microbial proteins. Furthermore, FRB supplementation suppressed markers of intestinal fibrosis. This effect might have been achieved via the canonical Smad2/3 activation and the non-canonical pathway of Tgf-β activity. These results suggest that FRB may be an alternative therapeutic agent against inflammation-induced intestinal fibrosis.

  33. L-Alanine Prototrophic Suppressors Emerge from L-Alanine Auxotroph through Stress-Induced Mutagenesis in Escherichia coli International-journal

    Harutaka Mishima, Hirokazu Watanabe, Kei Uchigasaki, So Shimoda, Shota Seki, Toshitaka Kumagai, Tomonori Nochi, Tasuke Ando, Hiroshi Yoneyama

    Microorganisms　9　(3)　472-472　2021/02/25

    Publisher: MDPI AG

    DOI： 10.3390/microorganisms9030472 　

    eISSN： 2076-2607

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    In Escherichia coli, L-alanine is synthesized by three isozymes: YfbQ, YfdZ, and AvtA. When an E. coli L-alanine auxotrophic isogenic mutant lacking the three isozymes was grown on L-alanine-deficient minimal agar medium, L-alanine prototrophic mutants emerged considerably more frequently than by spontaneous mutation; the emergence frequency increased over time, and, in an L-alanine-supplemented minimal medium, correlated inversely with L-alanine concentration, indicating that the mutants were derived through stress-induced mutagenesis. Whole-genome analysis of 40 independent L-alanine prototrophic mutants identified 16 and 18 clones harboring point mutation(s) in pyruvate dehydrogenase complex and phosphotransacetylase-acetate kinase pathway, which respectively produce acetyl coenzyme A and acetate from pyruvate. When two point mutations identified in L-alanine prototrophic mutants, in pta (D656A) and aceE (G147D), were individually introduced into the original L-alanine auxotroph, the isogenic mutants exhibited almost identical growth recovery as the respective cognate mutants. Each original- and isogenic-clone pair carrying the pta or aceE mutation showed extremely low phosphotransacetylase or pyruvate dehydrogenase activity, respectively. Lastly, extracellularly-added pyruvate, which dose-dependently supported L-alanine auxotroph growth, relieved the L-alanine starvation stress, preventing the emergence of L-alanine prototrophic mutants. Thus, L-alanine starvation-provoked stress-induced mutagenesis in the L-alanine auxotroph could lead to intracellular pyruvate increase, which eventually induces L-alanine prototrophy.

  34. In vivo emergence of beige-like fat in chickens as physiological adaptation to cold environments

    Rina Sotome, Akira Hirasawa, Motoi Kikusato, Taku Amo, Kyohei Furukawa, Anna Kuriyagawa, Kouichi Watanabe, Anne Collin, Hitoshi Shirakawa, Ryota Hirakawa, Yuta Tanitaka, Hideki Takahashi, Guoyao Wu, Tomonori Nochi, Tsuyoshi Shimmura, Craig H. Warden, Masaaki Toyomizu

    Amino Acids　53　(3)　381-393　2021/02/17

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00726-021-02953-5 　

    ISSN： 0939-4451

    eISSN： 1438-2199

  35. Interorgan Metabolism, Nutritional Impacts, and Safety of Dietary L-Glutamate and L-Glutamine in Poultry International-journal

    Wenliang He, Kyohei Furukawa, Masaaki Toyomizu, Tomonori Nochi, Christopher A. Bailey, Guoyao Wu

    Advances in Experimental Medicine and Biology　1332　107-128　2021

    Publisher: Springer International Publishing

    DOI： 10.1007/978-3-030-74180-8_7 　

    ISSN： 0065-2598

    eISSN： 2214-8019

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    L-glutamine (Gln) is the most abundant amino acid (AA) in the plasma and skeletal muscle of poultry, and L-glutamate (Glu) is among the most abundant AAs in the whole bodies of all avian tissues. During the first-pass through the small intestine into the portal circulation, dietary Glu is extensively oxidized to CO2, but dietary Gln undergoes limited catabolism in birds. Their extra-intestinal tissues (e.g., skeletal muscle, kidneys, and lymphoid organs) have a high capacity to degrade Gln. To maintain Glu and Gln homeostasis in the body, they are actively synthesized from branched-chain AAs (abundant AAs in both plant and animal proteins) and glucose via interorgan metabolism involving primarily the skeletal muscle, heart, adipose tissue, and brain. In addition, ammonia (produced from the general catabolism of AAs) and α-ketoglutarate (α-KG, derived primarily from glucose) serve as substrates for the synthesis of Glu and Gln in avian tissues, particularly the liver. Over the past 20 years, there has been growing interest in Glu and Gln metabolism in the chicken, which is an agriculturally important species and also a useful model for studying some aspects of human physiology and diseases. Increasing evidence shows that the adequate supply of dietary Glu and Gln is crucial for the optimum growth, anti-oxidative responses, productivity, and health of chickens, ducklings, turkeys, and laying fowl, particularly under stress conditions. Like mammals, poultry have dietary requirements for both Glu and Gln. Based on feed intake, tissue integrity, growth performance, and health status, birds can tolerate up to 12% Glu and 3.5% Gln in diets (on the dry matter basis). Glu and Gln are quantitatively major nutrients for chickens and other avian species to support their maximum growth, production, and feed efficiency, as well as their optimum health and well-being.

  36. Organogenesis of Ileal Peyer's Patches Is Initiated Prenatally and Accelerated Postnatally With Comprehensive Proliferation of B Cells in Pigs International-journal Peer-reviewed

    Mutsumi Furukawa, Shun Ito, Shunichi Suzuki, Daiichiro Fuchimoto, Akira Onishi, Kanae Niimi, Katsuki Usami, Guoyao Wu, Fuller W. Bazer, Kouetsu Ogasawara, Kouichi Watanabe, Hisashi Aso, Tomonori Nochi

    Frontiers in Immunology　11　604674-604674　2020/12/04

    Publisher: Frontiers Media SA

    DOI： 10.3389/fimmu.2020.604674 　

    eISSN： 1664-3224

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    Morphogenesis and differentiation of organs is required for subsequent functional maturation. The morphological features of Peyer's patches vary among species. In pigs, they develop extensively in the ileum as ileal Peyer's patches (IPPs). However, the role of IPPs in the porcine immune system remains to be elucidated because of a lack of complete understanding of IPP organogenesis. Results of the present study revealed that development of porcine IPPs is initiated prenatally between embryonic days 76 and 91. The process of IPP organogenesis is concomitant with increased transcriptional patterns of CXCL13 and CCL19. IPPs undergo further development postnatally by forming central, marginal, and subepithelial zones. Importantly, a large number of proliferating B cells and apoptotic cells are found in porcine IPPs postnatally, but not prenatally. The expression level of IgM in proliferating B cells depends on the zone in which distinct B cells are separately localized after birth. Specifically, IgM+ cells are predominantly found in the central zone, whereas IgM-/low cells are abundant in the marginal zone. Importantly, the cellular feature of IPPs differs from that of mesenteric lymph nodes (MLNs) where such distinct zones are not formed both prenatally and postnatally. Our findings suggest that IPPs (not MLNs) in postnatal pigs are involved in complementing functions of the primary lymphoid tissue that promotes the differentiation and maturation of B cells.

  37. Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies International-journal Peer-reviewed

    Binghui Zhou, Leonardo Albarracin, Yuhki Indo, Lorena Arce, Yuki Masumizu, Mikado Tomokiyo, Md. Aminul Islam, Valeria Garcia-Castillo, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Hidetoshi Morita, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa

    Microorganisms　8　(11)　1659-1659　2020/10/26

    Publisher: MDPI AG

    DOI： 10.3390/microorganisms8111659 　

    eISSN： 2076-2607

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    In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different “immune” and “adhesion” phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.

  38. Norovirus-specific immunoglobulin A in breast milk for protection against norovirus-associated diarrhea among infants International-journal

    Hannah Karen Mina Labayo, Monica J. Pajuelo, Kentaro Tohma, Lauren A. Ford-Siltz, Robert H. Gilman, Lilia Cabrera, Holger Mayta, Gerardo J. Sanchez, Anniuska Toledo Cornejo, Caryn Bern, Clyde Dapat, Tomonori Nochi, Gabriel I. Parra, Hitoshi Oshitani, Mayuko Saito

    EClinicalMedicine　27　100561-100561　2020/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.eclinm.2020.100561 　

    ISSN： 2589-5370

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    Background: Norovirus (NV) causes acute gastroenteritis in infants. Humoral and fecal immunoglobulin A (IgA) responses have been correlated with protection against NV; however, the role of breast milk IgA against NV infection and associated diarrhea is still unknown. This study aimed to evaluate the protective role of NV-specific IgA (NV-IgA) in breast milk. Methods: Ninety-five breast milk samples collected from mothers enrolled in a 2016-2017 Peruvian birth cohort study were tested for total IgA and NV-IgA by ELISA using GII·4 variants and non-GII·4 genotype virus-like particles (VLPs). Breast milk samples were grouped according to the NV infection and diarrheal status of infants: NV positive with diarrhea (NV+D+, n=18); NV positive without diarrhea (NV+D-, n=37); and NV negative without diarrhea (NV-D-, n=40). The percent positivity and titer of NV-IgA were compared among groups. The cross-reactivity was estimated based on the correlation of ratio between NV-IgA against GII·4 variants and non-GII·4 genotype VLPs. Findings: NV-IgA had high positivity rates against different VLPs, especially against GII (89-100%). The NV+D- group had higher percent positivity (89% vs. 61%, p=0·03) and median titer (1:100 vs 1:50, p=0·03) of NV-IgA than the NV+D+ group against GI·1 VLPs. A relatively high correlation between different GII·4 variants (0·87) and low correlation between genogroups (0·23-0·37) were observed. Interpretation: Mothers with high positivity rates and titers of NV-IgA in breast milk had NV infected infants with reduced diarrheal symptoms. Antigenic relatedness to the genetic diversity of human norovirus was suggested.Funding National Institute of Allergy and Infectious Diseases, National Institute of Health: 1R01AI108695-01A1 and the Japan Society for the Promotion of Science (Fostering Joint International Research B):19KK0241.

  39. Transcriptome Analysis of The Inflammatory Responses of Bovine Mammary Epithelial Cells: Exploring Immunomodulatory Target Genes for Bovine Mastitis. International-journal Peer-reviewed

    Md Aminul Islam, Michihiro Takagi, Kohtaro Fukuyama, Ryoya Komatsu, Leonardo Albarracin, Tomonori Nochi, Yoshihito Suda, Wakako Ikeda-Ohtsubo, Victor Rutten, Willem van Eden, Julio Villena, Hisashi Aso, Haruki Kitazawa

    Pathogens (Basel, Switzerland)　9　(3)　200-200　2020/03/09

    Publisher: MDPI AG

    DOI： 10.3390/pathogens9030200 　

    eISSN： 2076-0817

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    Bovine mastitis is the inflammatory reaction of the mammary gland and is commonly caused by bacterial infections in high-yielding dairy cows. The detailed investigation of the immunotranscriptomic response of bovine mammary epithelial (BME) cells to pattern recognition receptors (PRRs) activation by microbial-associated molecular patterns (MAMPs) can be of great importance for understanding the innate immune defense mechanisms, and for exploring the immunomodulatory candidate genes. In this work, we investigated the transcriptome modifications of BME cells after the in vitro stimulation with Escherichia coli derived lipopolysaccharide (LPS) and heat-killed Staphylococcusaureus JE2 and S. aureus SA003. In addition, the effect of Pam3CSK4 (a synthetic triacylated lipopeptide that activates Toll-like receptor 2 (TLR2)), and the intracellular chemotactic protein cyclophilin A (CyPA), which is secreted by BME cells during mastitis, in the expression changes of selected cytokines and chemokines were evaluated by qPCR. Microarray analysis identified 447, 465 and 520 differentially expressed genes (DEGs) in the BME cells after LPS, S. aureus JE2 and S. aureus SA003 stimulation, respectively. A major differential response in the inflammatory gene expression was noticed between the stimulation of LPS and S. aureus strains. Unlike the S. aureus strains, LPS stimulation resulted in significant upregulation of CCL2, CXCL2, CXCL3, CXCL8,IL1 and IL1, which were confirmed by qPCR analysis. Pam3CSK4 was not able to induce significant changes in the expression of cytokines and chemokines in challenged BME cells. The exogenous CyPA administration was able to upregulate CXCL2, CXCL3, CXCL8, IL1 and IL1 expression in BME cells indicating its ability to promote inflammation. The identification of transcriptional markers of mastitis specific for individual inflammatory factors such as LPS, Pam3CSK4 or CyPA, which can be evaluated in vitro in BME cells, may enable the development of novel diagnostics and/or immunomodulatory treatments, providing new tools for the effective management of mastitis in dairy cows. The results of this work are an advance in this regard.

  40. Editorial: New Horizons in Food Science via Agricultural Immunity Peer-reviewed

    Tomonori Nochi, Corné M. J. Pieterse, Willem Van Eden

    Frontiers in Nutrition　7　2020/02/28

    Publisher: Frontiers Media SA

    DOI： 10.3389/fnut.2020.00019 　

    eISSN： 2296-861X

  41. Heat Stress Causes Immune Abnormalities via Massive Damage to Effect Proliferation and Differentiation of Lymphocytes in Broiler Chickens Peer-reviewed

    Ryota Hirakawa, Siti Nurjanah, Kyohei Furukawa, Atsushi Murai, Motoi Kikusato, Tomonori Nochi, Masaaki Toyomizu

    Frontiers in Veterinary Science　7　2020/02/07

    DOI： 10.3389/fvets.2020.00046 　

    eISSN： 2297-1769

  42. IL‐12p40 gene expression in lung and hilar lymph nodes of MPS‐resistant pigs International-journal Peer-reviewed

    Akiko Sakuma, Shizuka Sugawara, Hikaru Hidaka, Mitsuru Nakajo, Yoshihito Suda, Tomoyuki Shimazu, Michael T. Rose, Megumi Urakawa, Tao Zhuang, Guoqi Zhao, Kouichi Watanabe, Tomonori Nochi, Haruki Kitazawa, Kazuo Katoh, Keiichi Suzuki, Hisashi Aso

    Animal Science Journal　91　(1)　e13450　2020/01

    Publisher: Wiley

    DOI： 10.1111/asj.13450 　

    ISSN： 1344-3941

    eISSN： 1740-0929

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    Mycoplasma pneumonia of swine (MPS) is caused by Mycoplasma hyopneumoniae (M.hp) and is a common chronic respiratory disease of pigs. Recently, a genetically selected variant of the Landrace pig (Miyagino L2) has a lower incidence of pulmonary MPS lesions. We investigated the pathological and immunological characteristics of MPS resistance in these pigs (n = 24) by comparing with the normal landrace pig (control: n = 24). The pathological MPS lung lesion score in MPS-selected landrace pigs was significantly lower than in the control. The gene expression of interleukin (IL)-12p40, which acts as a chemoattractant and a component of the bioactive cytokines IL-12 and IL-23, was significantly higher at the hilar lymph nodes, lung, and spleen in MPS-selected landrace pigs than in control landrace pigs, and these were negatively correlated with the macroscopic MPS lung lesion score. In summary, we demonstrate that resistance against MPS in Miyagino L2 pigs is associated with IL-12p40 up-regulation, in comparison with normal landrace pigs without the MPS vaccine. In addition, a comparative study of macroscopic MPS lung lesions and IL-12p40 gene expression in lung and hilar lymph nodes may lead to beneficial selection traits for the genetic selection for MPS resistance in pigs.

  43. Paraimmunobiotic Bifidobacteria Modulate the Expression Patterns of Peptidoglycan Recognition Proteins in Porcine Intestinal Epitheliocytes and Antigen Presenting Cells. International-journal Peer-reviewed

    Hikaru Iida, Masanori Tohno, Md Aminul Islam, Nana Sato, Hisakazu Kobayashi, Leonardo Albarracin, Akm Humayun Kober, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Tomonori Nochi, Ayako Miyazaki, Hirohide Uenishi, Noriyuki Iwabuchi, Jin-Zhong Xiao, Julio Villena, Haruki Kitazawa

    Cells　8　(8)　1-22　2019/08/14

    DOI： 10.3390/cells8080891 　

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    Peptidoglycan recognition proteins (PGLYRPs) are a family of pattern recognition receptors (PRRs) that are able to induce innate immune responses through their binding to peptidoglycan (PGN), lipopolysaccharide, or lipoteichoic acid, or by interacting with other PRR-ligands. Recently, progress has been made in understanding the immunobiology of PGLYRPs in human and mice, however, their functions in livestock animals have been less explored. In this study, we characterized the expression patterns of PGLYRPs in porcine intestinal epithelial (PIE) cells and antigen-presenting cells (APCs) and their modulation by the interactions of host cells with PRR-ligands and non-viable immunomodulatory probiotics referred to as paraimmunobiotics. We demonstrated that PGLYRP-1, -2, -3, and -4 are expressed in PIE cells and APCs from Peyer's patches, being PGLYPR-3 and -4 levels higher than PGLYRP-1 and -2. We also showed that PGLYRPs expression in APCs and PIE cells can be modulated by different PRR agonists. By using knockdown PIE cells for TLR2, TLR4, NOD1, and NOD2, or the four PGLYRPs, we demonstrated that PGLYRPs expressions would be required for activation and functioning of TLR2, TLR4, NOD1, and NOD2 in porcine epitheliocytes, but PGLYRPs activation would be independent of those PRR expressions. Importantly, we reported for the first time that PGLYRPs expression can be differentially modulated by paraimmunobiotic bifidobacteria in a strain-dependent manner. These results provide evidence for the use of paraimmunobiotic bifidobacteria as an alternative for the improvement of resistance to intestinal infections or as therapeutic tools for the reduction of the severity of inflammatory damage in diseases in which a role of PGLYRPs-microbe interaction has been demonstrated.

  44. Staphylococcus aureus-specific IgA antibody in milk suppresses the multiplication of S. aureus in infected bovine udder International-journal Peer-reviewed

    Yuya Nagasawa, Yoshio Kiku, Kazue Sugawara, Aya Hirose, Chiaki Kai, Nana Kitano, Toshihiko Takahashi, Tomonori Nochi, Hisashi Aso, Shin Ichi Sawada, Kazunari Akiyoshi, Tomohito Hayashi

    BMC Veterinary Research　15　(1)　286-286　2019/08/09

    DOI： 10.1186/s12917-019-2025-3 　

    eISSN： 1746-6148

  45. Microbiota maintain colonic homeostasis by activating TLR2/MyD88/PI3K signaling in IL-10–producing regulatory B cells Peer-reviewed

    Yoshiyuki Mishima, Akihiko Oka, Bo Liu, Jeremy W. Herzog, Chang Soo Eun, Ting-Jia Fan, Emily Bulik-Sullivan, Ian M. Carroll, Jonathan J. Hansen, Liang Chen, Justin E. Wilson, Nancy C. Fisher, Jenny P.Y. Ting, Tomonori Nochi, Angela Wahl, J. Victor Garcia, Christopher L. Karp, R. Balfour Sartor

    Journal of Clinical Investigation　2019/08/05

    Publisher: American Society for Clinical Investigation

    DOI： 10.1172/JCI93820 　

  46. Isolation and Immunocharacterization of Lactobacillus salivarius from the Intestine of Wakame-Fed Pigs to Develop Novel "Immunosynbiotics". International-journal Peer-reviewed

    Yuki Masumizu, Binghui Zhou, A K M Humayun Kober, Md Aminul Islam, Hikaru Iida, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Leonardo Albarracin, Tomonori Nochi, Hisashi Aso, Keiichi Suzuki, Julio Villena, Haruki Kitazawa

    Microorganisms　7　(6)　1-17　2019/06/06

    DOI： 10.3390/microorganisms7060167 　

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    Emerging threats of antimicrobial resistance necessitate the exploration of effective alternatives for healthy livestock growth strategies. 'Immunosynbiotics', a combination of immunoregulatory probiotics and prebiotics with synergistic effects when used together in feed, would be one of the most promising candidates. Lactobacilli are normal residents of the gastrointestinal tract of pigs, and many of them are able to exert beneficial immunoregulatory properties. On the other hand, wakame (Undaria pinnafida), an edible seaweed, has the potential to be used as an immunoregulatory prebiotic when added to livestock feed. Therefore, in order to develop a novel immunosynbiotic, we isolated and characterized immunoregulatory lactobacilli with the ability to utilize wakame. Following a month-long in vivo wakame feeding trial in 8-week-old Landrace pigs (n = 6), sections of intestinal mucous membrane were processed for bacteriological culture and followed by identification of pure colonies by 16S rRNA sequence. Each isolate was characterized in vitro in terms of their ability to assimilate to the wakame and to differentially modulate the expression of interleukin-6 (IL-6) and interferon beta (IFN-β) in the porcine intestinal epithelial (PIE) cells triggered by Toll-like receptor (TLR)-4 and TLR-3 activation, respectively. We demonstrated that feeding wakame to pigs significantly increased the lactobacilli population in the small intestine. We established a wakame-component adjusted culture media that allowed the isolation and characterization of a total of 128 Lactobacilli salivarius colonies from the gut of wakame-fed pigs. Interestingly, several L. salivarius isolates showed both high wakame assimilation ability and immunomodulatory capacities. Among the wakame assimilating isolates, L. salivarius FFIG71 showed a significantly higher capacity to upregulate the IL-6 expression, and L. salivarius FFIG131 showed significantly higher capacity to upregulate the IFN-β expression; these could be used as immunobiotic strains in combination with wakame for the development of novel immunologically active feeds for pigs.

  47. Gut microbiota development in mice is affected by hydrogen peroxide produced from amino acid metabolism during lactation Peer-reviewed

    Yuko Shigeno, Haolin Zhang, Taihei Banno, Kento Usuda, Tomonori Nochi, Ryo Inoue, Gen Watanabe, Wanzhu Jin, Yoshimi Benno, Kentaro Nagaoka

    The FASEB Journal　33　(3)　3343-3352　2019/03

    Publisher: Wiley

    DOI： 10.1096/fj.201801462r 　

    ISSN： 0892-6638

    eISSN： 1530-6860

  48. The Well-Developed Mucosal Immune Systems of Birds and Mammals Allow for Similar Approaches of Mucosal Vaccination in Both Types of Animals Peer-reviewed

    Tomonori Nochi, Christine A. Jansen, Masaaki Toyomizu, Willem van Eden

    Frontiers in Nutrition　5　2018/07/12

    Publisher: Frontiers Media SA

    DOI： 10.3389/fnut.2018.00060 　

    eISSN： 2296-861X

  49. Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method. International-journal Peer-reviewed

    Tao Zhuang, Megumi Urakawa, Hidetoshi Sato, Yuko Sato, Teruaki Taguchi, Tsuyoshi Umino, Shiro Katto, Koutaro Tanaka, Kozue Yoshimura, Naokazu Takada, Hiroko Kobayashi, Megumi Ito, Michael T Rose, Yoshio Kiku, Yuya Nagasawa, Haruki Kitazawa, Kouichi Watanabe, Tomonori Nochi, Tomohito Hayashi, Hisashi Aso

    Animal science journal = Nihon chikusan Gakkaiho　89　(7)　1011-1019　2018/07

    DOI： 10.1111/asj.13014 　

    ISSN： 1344-3941

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    Dendritic cells (DCs) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DCs was identified as CD172a+ /CD11c+ /MHC (major histocompatibility complex) class II+ cells. Although DCs are identified at 0.1%-0.7% of peripheral blood mononuclear cells (PBMC), the phenotype and function of DCs remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DCs from PBMC using magnetic-activated cell sorting, and purified the CD172a+ /CD11c+ DCs, with high expression of MHC class II and CD40, at 84.8% purity. There were individual differences in the expressions of CD205 and co-stimulatory molecules CD80 and CD86 on DCs. There were positive correlations between expression of cytokine and co-stimulatory molecules in DCs, and the DCs maintained their immune tolerance, evidenced by their low expressions of the co-stimulatory molecules and cytokine production. These results suggest that before parturition a half of DCs may be immature and tend to maintain tolerance based on the low cytokine production, and the other DCs with high co-stimulatory molecules may already have the ability of modulating the T-cell linage.

  50. Effects of a moderate-fat diet that is enriched with fish oil on intestinal lipid absorption in a senescence-accelerated prone mouse model Peer-reviewed

    Kazushi Yamamoto, Yui Iwagaki, Kouichi Watanabe, Tomonori Nochi, Hisashi Aso, Tsuyoshi Tsuduki

    Nutrition　50　26-35　2018/06/01

    Publisher: Elsevier Inc.

    DOI： 10.1016/j.nut.2017.10.015 　

    ISSN： 1873-1244 0899-9007

  51. Lipopolysaccharide (LPS)-binding protein stimulates CD14-dependent Toll-like receptor 4 internalization and LPS-induced TBK1-IKKϵ-IRF3 axis activation. International-journal Peer-reviewed

    Tsukamoto H, Takeuchi S, Kubota K, Kobayashi Y, Kozakai S, Ukai I, Shichiku A, Okubo M, Numasaki M, Kanemitsu Y, Matsumoto Y, Nochi T, Watanabe K, Aso H, Tomioka Y

    The Journal of biological chemistry　293　(26)　10186-10201　2018/05

    DOI： 10.1074/jbc.M117.796631 　

    ISSN： 0021-9258

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    Toll-like receptor 4 (TLR4) is an indispensable immune receptor for lipopolysaccharide (LPS), a major component of the Gram-negative bacterial cell wall. Following LPS stimulation, TLR4 transmits the signal from the cell surface and becomes internalized in an endosome. However, the spatial regulation of TLR4 signaling is not fully understood. Here, we investigated the mechanisms of LPS-induced TLR4 internalization and clarified the roles of the extracellular LPS-binding molecules, LPS-binding protein (LBP), and glycerophosphatidylinositol-anchored protein (CD14). LPS stimulation of CD14-expressing cells induced TLR4 internalization in the presence of serum, and an inhibitory anti-LBP mAb blocked its internalization. Addition of LBP to serum-free cultures restored LPS-induced TLR4 internalization to comparable levels of serum. The secretory form of the CD14 (sCD14) induced internalization but required a much higher concentration than LBP. An inhibitory anti-sCD14 mAb was ineffective for serum-mediated internalization. LBP lacking the domain for LPS transfer to CD14 and a CD14 mutant with reduced LPS binding both attenuated TLR4 internalization. Accordingly, LBP is an essential serum molecule for TLR4 internalization, and its LPS transfer to membrane-anchored CD14 (mCD14) is a prerequisite. LBP induced the LPS-stimulated phosphorylation of TBK1, IKKϵ, and IRF3, leading to IFN-β expression. However, LPS-stimulated late activation of NF-κB or necroptosis were not affected. Collectively, our results indicate that LBP controls LPS-induced TLR4 internalization, which induces TLR adaptor molecule 1 (TRIF)-dependent activation of the TBK1-IKKϵ-IRF3-IFN-β pathway. In summary, we showed that LBP-mediated LPS transfer to mCD14 is required for serum-dependent TLR4 internalization and activation of the TRIF pathway.

  52. Identification of a novel mechanism of action of bovine IgG antibodies specific for Staphylococcus aureus. International-journal Peer-reviewed

    Mutsumi Furukawa, Hiroshi Yoneyama, Eiji Hata, Hidetomo Iwano, Hidetoshi Higuchi, Tasuke Ando, Mika Sato, Tomohito Hayashi, Yoshio Kiku, Yuya Nagasawa, Kanae Niimi, Katsuki Usami, Kumiko Ito, Kouichi Watanabe, Tomonori Nochi, Hisashi Aso

    Veterinary research　49　(1)　22-22　2018/02/26

    DOI： 10.1186/s13567-018-0517-y 　

    ISSN： 0928-4249

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    Staphylococcus aureus is a major pathogen that causes subclinical mastitis associated with huge economic losses to the dairy industry. A few vaccines for bovine mastitis are available, and they are expected to induce the production of S. aureus-specific antibodies that prevent bacterial adherence to host cells or promote opsonization by phagocytes. However, the efficacy of such vaccines are still under debate; therefore, further research focusing on improving the current vaccines by seeking additional mechanisms of action is required to reduce economic losses due to mastitis in the dairy industry. Here, we generated S. aureus-specific bovine IgG antibodies (anti-S. aureus) that directly inhibited bacterial growth in vitro. Inhibition depended on specificity for anti-S. aureus, not the interaction between Protein A and the fragment crystallizable region of the IgG antibodies or bacterial agglutination. An in vitro culture study using S. aureus strain JE2 and its deletion mutant JE2ΔSrtA, which lacks the gene encoding sortase A, revealed that the effect of anti-S. aureus was sortase-A-independent. Sortase A is involved in the synthesis of cell-wall-associated proteins. Thus, other surface molecules, such as membrane proteins, cell surface polysaccharides, or both, may trigger the inhibition of bacterial growth by anti-S. aureus. Together, our findings contribute insights into developing new strategies to further improve the available mastitis vaccine by designing a novel antigen on the surface of S. aureus to induce inhibitory signals that prevent bacterial growth.

  53. Transcriptomic analysis of the innateantiviral immune response in Porcine intestinal epithelial cells: influence of immunobiotic lactobacilli Peer-reviewed

    Leonardo Albarracin, Hisakazu Kobayashi, Hikaru Iida, Nana Sato, Tomonori Nochi, Hisashi Aso, Susana Salva, Susana Alvarez, Haruki Kitazawa, Julio Villena

    Frontiers in Immunology　8　57　2017/02

    DOI： 10.3389/fimmu.2017.00057 　

    ISSN： 1664-3224

  54. Critical role of intestinal interleukin-4 modulating regulatory T cells for desensitization, tolerance, and inflammation of food allergy Peer-reviewed

    Haruyo Nakajima-Adachi, Kyoko Shibahara, Yoko Fujimura, Jun Takeyama, Erika Hiraide, Akira Kikuchi, Hitoshi Murakami, Akira Hosono, Tomonori Nochi, Yoshio Wakatsuki, Naoki Shimojo, Shuichi Kaminogawa, Ryuichiro Sato, Hiroshi Kiyono, Satoshi Hachimura

    PLOS ONE　12　(2)　e0172795　2017/02

    DOI： 10.1371/journal.pone.0172795 　

    ISSN： 1932-6203

  55. Development of an in vitro immunobiotic evaluation system against rotavirus infection in bovine intestinal epitheliocytes Peer-reviewed

    H. Kobayashi, P. Kanmani, T. Ishizuka, A. Miyazaki, J. Soma, L. Albarracin, Y. Suda, T. Nochi, H. Aso, N. Iwabuchi, J. Z. Xiao, T. Saito, J. Villena, H. Kitazawa

    Beneficial Microbes　8　(2)　309-321　2017

    Publisher: Wageningen Academic Publishers

    DOI： 10.3920/BM2016.0155 　

    ISSN： 1876-2891 1876-2883

  56. Cyclophilin A is a new M cell marker of bovine intestinal epithelium Peer-reviewed

    Tetsuya Hondo, Shunsuke Someya, Yuya Nagasawa, Shunsuke Terada, Hitoshi Watanabe, Xiangning Chen, Kouichi Watanabe, Shyuichi Ohwada, Haruki Kitazawa, Michael T. Rose, Tomonori Nochi, Hisashi Aso

    CELL AND TISSUE RESEARCH　364　(3)　585-597　2016/06

    DOI： 10.1007/s00441-015-2342-1 　

    ISSN： 0302-766X

    eISSN： 1432-0878

  57. Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells Peer-reviewed

    Kohichiro Tsukida, Takuya Takahashi, Hikaru Iida, Paulraj Kanmani, Yoshihito Suda, Tomonori Nochi, Shuichi Ohwada, Hisashi Aso, Sou Ohkawara, Seiya Makino, Hiroshi Kano, Tadao Saito, Julio Villena, Haruki Kitazawa

    BMC IMMUNOLOGY　17　(1)　21　2016/06

    DOI： 10.1186/s12865-016-0160-1 　

    ISSN： 1471-2172

  58. Development of Human-Like Advanced Coronary Plaques in Low-Density Lipoprotein Receptor Knockout Pigs and Justification for Statin Treatment Before Formation of Atherosclerotic Plaques Peer-reviewed

    Yuxin Li, Daiichiro Fuchimoto, Mitsumasa Sudo, Hironori Haruta, Qing-Fei Lin, Tadateru Takayama, Shotaro Morita, Tomonori Nochi, Shunichi Suzuki, Shoichiro Sembon, Michiko Nakai, Misaki Kojima, Masaki Iwamoto, Michiko Hashimoto, Shunichi Yoda, Satoshi Kunimoto, Takafumi Hiro, Taro Matsumoto, Masako Mitsumata, Masahiko Sugitani, Satoshi Saito, Atsushi Hirayama, Akira Onishi

    JOURNAL OF THE AMERICAN HEART ASSOCIATION　5　(4)　e002779　2016/04

    DOI： 10.1161/JAHA.115.002779 　

    ISSN： 2047-9980

  59. ART influences HIV persistence in the female reproductive tract and cervicovaginal secretions Peer-reviewed

    Rikke Olesen, Michael D. Swanson, Martina Kovarova, Tomonori Nochi, Morgan Chateau, Jenna B. Honeycutt, Julie M. Long, Paul W. Denton, Michael G. Hudgens, Amy Richardson, Martin Tolstrup, Lars Ostergaard, Angela Wahl, J. Victor Garcia

    JOURNAL OF CLINICAL INVESTIGATION　126　(3)　892-904　2016/03

    DOI： 10.1172/JCI64212 　

    ISSN： 0021-9738

    eISSN： 1558-8238

  60. Immunobiotic Bifidobacteria Strains Modulate Rotavirus Immune Response in Porcine Intestinal Epitheliocytes via Pattern Recognition Receptor Signaling Peer-reviewed

    Takamasa Ishizuka, Paulraj Kanmani, Hisakazu Kobayashi, Ayako Miyazaki, Junichi Soma, Yoshihito Suda, Hisashi Aso, Tomonori Nochi, Noriyuki Iwabuchi, Jin-zhong Xiao, Tadao Saito, Julio Villena, Haruki Kitazawa

    PLOS ONE　11　(3)　e0152416　2016/03

    DOI： 10.1371/journal.pone.0152416 　

    ISSN： 1932-6203

  61. Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937 Peer-reviewed

    H. Kobayashi, L. Albarracin, N. Sato, P. Kanmani, A. K.M.H. Kober, W. Ikeda-Ohtsubo, Y. Suda, T. Nochi, H. Aso, S. Makino, H. Kano, S. Ohkawara, T. Saito, J. Villena, H. Kitazawa

    Beneficial Microbes　7　(5)　769-782　2016

    Publisher: Wageningen Academic Publishers

    DOI： 10.3920/BM2016.0095 　

    ISSN： 1876-2891 1876-2883

  62. Extracellular cyclophilin A possesses chemotaxic activity in cattle. International-journal Peer-reviewed

    Satoru Takanashi, Tomonori Nochi, Miku Abe, Nanami Itaya, Megumi Urakawa, Katsuyoshi Sato, Tao Zhuang, Saori Umemura, Tomohito Hayashi, Yoshio Kiku, Haruki Kitazawa, Michael T Rose, Kouichi Watanabe, Hisashi Aso

    Veterinary research　46　(1)　80-80　2015/07/11

    DOI： 10.1186/s13567-015-0212-1 　

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    Cyclophilin A (CyPA) was originally discovered in bovine thymocytes as a cytosolic binding protein of the immunosuppressive drug cyclosporine A. Recent studies have revealed that in mice and humans, CyPA is secreted from cells in injured or infected tissues and plays a role in recruiting inflammatory cells in those tissues. Here we found that in cattle abundant level of extracellular CyPA was observed in tissues with inflammation. To aid in investigating the role of extracellular CyPA in cattle, we generated recombinant bovine CyPA (rbCyPA) and tested its biological activity as an inflammatory mediator. When bovine peripheral blood cells were treated with rbCyPA in vitro, we observed that rbCyPA reacts with the membranous surface of granulocytes, monocytes and lymphocytes. Chemotaxis analysis showed that the granulocytes migrate toward rbCyPA and the migration is inhibited by pre-treatment with an anti-bovine CyPA antibody. These results indicate that, as for mice and humans, extracellular CyPA possesses chemotactic activity to recruit inflammatory cells (e.g., granulocytes) in cattle, and could thus be a potential therapeutic target for the treatment of inflammation.

  63. Prion protein binds to aldolase A produced by bovine intestinal M cells Peer-reviewed

    Nagasawa Y, Takahashi Y, Itani W, Watanabe H, Hidaka Y, Morita S, Suzuki K, Watanabe K, Ohwada S, Kitazawa H, Imamura M, Yokoyama T, Horiuchi M, Sakaguchi S, Mohri S, Rise MT, Nochi T, Aso H

    Scientific Res.　5　43-60　2015/03/11

    DOI： 10.4236/ojvm.2015.53007 　

  64. Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics Peer-reviewed

    Masahiko Suzuki, Asuka Tada, Paulraj Kanmani, Hitoshi Watanabe, Hisashi Aso, Yoshihito Suda, Tomonori Nochi, Kenji Miyazawa, Kazutoyo Yoda, Fang He, Masataka Hosoda, Tadao Saito, Julio Villena, Haruki Kitazawa

    PLOS ONE　10　(3)　e0119644　2015/03

    DOI： 10.1371/journal.pone.0119644 　

    ISSN： 1932-6203

  65. Vaginal Memory T Cells Induced by Intranasal Vaccination Are Critical for Protective T Cell Recruitment and Prevention of Genital HSV-2 Disease Peer-reviewed

    Ayuko Sato, Aldina Suwanto, Manami Okabe, Shintaro Sato, Tomonori Nochi, Takahiko Imai, Naoto Koyanagi, Jun Kunisawa, Yasushi Kawaguchi, Hiroshi Kiyono

    JOURNAL OF VIROLOGY　88　(23)　13699-13708　2014/12

    DOI： 10.1128/JVI.02279-14 　

    ISSN： 0022-538X

    eISSN： 1098-5514

  66. Specific Expression of Apolipoprotein A-IV in the Follicle-Associated Epithelium of the Small Intestine Peer-reviewed

    Daisuke Tokuhara, Tomonori Nochi, Akiko Matsumura, Mio Mejima, Yuko Takahashi, Shiho Kurokawa, Hiroshi Kiyono, Yoshikazu Yuki

    DIGESTIVE DISEASES AND SCIENCES　59　(11)　2682-2692　2014/11

    DOI： 10.1007/s10620-014-3203-6 　

    ISSN： 0163-2116

    eISSN： 1573-2568

  67. Hypogammaglobulinemia in BLT Humanized Mice - An Animal Model of Primary Antibody Deficiency Peer-reviewed

    Francisco Martinez-Torres, Tomonori Nochi, Angela Wahl, J. Victor Garcia, Paul W. Denton

    PLOS ONE　9　(10)　e108663　2014/10

    DOI： 10.1371/journal.pone.0108663 　

    ISSN： 1932-6203

  68. Peyer's Patches and Mesenteric Lymph Nodes Cooperatively Promote Enteropathy in a Mouse Model of Food Allergy Peer-reviewed

    Haruyo Nakajima-Adachi, Akira Kikuchi, Yoko Fujimura, Kyoko Shibahara, Tsuyoshi Makino, Masae Goseki-Sone, Miran Kihara-Fujioka, Tomonori Nochi, Yosuke Kurashima, Osamu Igarashi, Masafumi Yamamoto, Jun Kunisawa, Masako Toda, Shuichi Kaminogawa, Ryuichiro Sato, Hiroshi Kiyono, Satoshi Hachimura

    PLOS ONE　9　(10)　e107492　2014/10

    DOI： 10.1371/journal.pone.0107492 　

    ISSN： 1932-6203

  69. NFIL3-Deficient Mice Develop Microbiota-Dependent, IL-12/23-Driven Spontaneous Colitis Peer-reviewed

    Taku Kobayashi, Erin C. Steinbach, Steven M. Russo, Katsuyoshi Matsuoka, Tomonori Nochi, Nitsan Maharshak, Luke B. Borst, Bruce Hostager, J. Victor Garcia-Martinez, Paul B. Rothman, Masaki Kashiwada, Shehzad Z. Sheikh, Peter J. Murray, Scott E. Plevy

    JOURNAL OF IMMUNOLOGY　192　(4)　1918-1927　2014/02

    DOI： 10.4049/jimmunol.1301819 　

    ISSN： 0022-1767

    eISSN： 1550-6606

  70. Rice-based oral antibody fragment prophylaxis and therapy against rotavirus infection Peer-reviewed

    Daisuke Tokuhara, Beatriz Alvarez, Mio Mejima, Tomoko Hiroiwa, Yuko Takahashi, Shiho Kurokawa, Masaharu Kuroda, Masaaki Oyama, Hiroko Kozuka-Hata, Tomonori Nochi, Hiroshi Sagara, Farah Aladin, Harold Marcotte, Leon G. J. Frenken, Miren Iturriza-Gomara, Hiroshi Kiyono, Lennart Hammarstrom, Yoshikazu Yuki

    JOURNAL OF CLINICAL INVESTIGATION　123　(9)　3829-3838　2013/09

    DOI： 10.1172/JCI70266 　

    ISSN： 0021-9738

  71. Induction of toxin-specific neutralizing immunity by molecularly uniform rice-based oral cholera toxin B subunit vaccine without plant-associated sugar modification Peer-reviewed

    Yoshikazu Yuki, Mio Mejima, Shiho Kurokawa, Tomoko Hiroiwa, Yuko Takahashi, Daisuke Tokuhara, Tomonori Nochi, Yuko Katakai, Masaharu Kuroda, Natsumi Takeyama, Koji Kashima, Michiyo Abe, Yingju Chen, Ushio Nakanishi, Takehiro Masumura, Yoji Takeuchi, Hiroko Kozuka-Hata, Hiroaki Shibata, Masaaki Oyama, Kunisuke Tanaka, Hiroshi Kiyono

    PLANT BIOTECHNOLOGY JOURNAL　11　(7)　799-808　2013/09

    DOI： 10.1111/pbi.12071 　

    ISSN： 1467-7644

  72. Nanogel-Based PspA Intranasal Vaccine Prevents Invasive Disease and Nasal Colonization by Streptococcus pneumoniae Peer-reviewed

    Il Gyu Kong, Ayuko Sato, Yoshikazu Yuki, Tomonori Nochi, Haruko Takahashi, Shinichi Sawada, Mio Mejima, Shiho Kurokawa, Kazunari Okada, Shintaro Sato, David E. Briles, Jun Kunisawa, Yusuke Inoue, Masafumi Yamamoto, Kazunari Akiyoshi, Hiroshi Kiyono

    INFECTION AND IMMUNITY　81　(5)　1625-1634　2013/05

    DOI： 10.1128/IAI.00240-13 　

    ISSN： 0019-9567

    eISSN： 1098-5522

  73. Nanogel-based antigen-delivery system for nasal vaccines Peer-reviewed

    Yoshikazu Yukia, Tomonori Nochi, Il Gyu Kong, Haruko Takahashi, Shin-Ichi Sawada, Kazunari Akiyoshi, Hiroshi Kiyono

    Biotechnology and Genetic Engineering Reviews　29　(1)　61-72　2013

    Publisher: Informa UK Limited

    DOI： 10.1080/02648725.2013.801226 　

    ISSN： 0264-8725

    eISSN： 2046-5556

  74. IL-2 receptor γ-chain molecule is critical for intestinal T-cell reconstitution in humanized mice Peer-reviewed

    P W Denton, T Nochi, A Lim, J F Krisko, F Martinez-Torres, S K Choudhary, A Wahl, R Olesen, W Zou, J P Di Santo, D M Margolis, J V Garcia

    Mucosal Immunology　5　(5)　555-566　2012/09

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/mi.2012.31 　

    ISSN： 1933-0219

    eISSN： 1935-3456

  75. Human Breast Milk and Antiretrovirals Dramatically Reduce Oral HIV-1 Transmission in BLT Humanized Mice Peer-reviewed

    Angela Wahl, Michael D. Swanson, Tomonori Nochi, Rikke Olesen, Paul W. Denton, Morgan Chateau, J. Victor Garcia

    PLoS Pathogens　8　(6)　e1002732-e1002732　2012/06/14

    Publisher: Public Library of Science (PLoS)

    DOI： 10.1371/journal.ppat.1002732 　

    eISSN： 1553-7374

  76. RNAi suppression of rice endogenous storage proteins enhances the production of rice-based Botulinum neutrotoxin type A vaccine Peer-reviewed

    Yoshikazu Yuki, Mio Mejima, Shiho Kurokawa, Tomoko Hiroiwa, Il Gyu Kong, Masaharu Kuroda, Yoko Takahashi, Tomonori Nochi, Daisuke Tokuhara, Tomoko Kohda, Shunji Kozaki, Hiroshi Kiyono

    Vaccine　30　(28)　4160-4166　2012/06/13

    Publisher: Elsevier BV

    DOI： 10.1016/j.vaccine.2012.04.064 　

    ISSN： 0264-410X 1873-2518

  77. Human Breast Milk and Antiretrovirals Dramatically Reduce Oral HIV-1 Transmission in BLT Humanized Mice Peer-reviewed

    Angela Wahl, Michael D. Swanson, Tomonori Nochi, Rikke Olesen, Paul W. Denton, Morgan Chateau, J. Victor Garcia

    PLOS PATHOGENS　8　(6)　2012/06

    DOI： 10.1371/journal.ppat.1002732 　

    ISSN： 1553-7374

  78. Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors Peer-reviewed

    Yosuke Kurashima, Takeaki Amiya, Tomonori Nochi, Kumiko Fujisawa, Takeshi Haraguchi, Hideo Iba, Hiroko Tsutsui, Shintaro Sato, Sachiko Nakajima, Hideki Iijima, Masato Kubo, Jun Kunisawa, Hiroshi Kiyono

    Nature Communications　3　2012

    DOI： 10.1038/ncomms2023 　

    ISSN： 2041-1723

  79. Generation of HIV Latency in Humanized BLT Mice Peer-reviewed

    P. W. Denton, R. Olesen, S. K. Choudhary, N. M. Archin, A. Wahl, M. D. Swanson, M. Chateau, T. Nochi, J. F. Krisko, R. A. Spagnuolo, D. M. Margolis, J. V. Garcia

    Journal of Virology　86　(1)　630-634　2012/01/01

    Publisher: American Society for Microbiology

    DOI： 10.1128/jvi.06120-11 　

    ISSN： 0022-538X

  80. Nef functions in BLT mice to enhance HIV-1 replication and deplete CD4+CD8+ thymocytes Peer-reviewed

    Wei Zou, Paul W Denton, Richard L Watkins, John F Krisko, Tomonori Nochi, John L Foster, J Garcia

    Retrovirology　9　(1)　44-44　2012

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1186/1742-4690-9-44 　

    ISSN： 1742-4690

  81. M cells expressing the complement C5a receptor are efficient targets for mucosal vaccine delivery Peer-reviewed

    Sae-Hae Kim, Dae-Im Jung, In-Young Yang, Ju Kim, Kyung-Yeol Lee, Tomonori Nochi, Hiroshi Kiyono, Yong-Suk Jang

    European Journal of Immunology　41　(11)　3219-3229　2011/11

    Publisher: Wiley

    DOI： 10.1002/eji.201141592 　

    ISSN： 0014-2980 1521-4141

  82. The Airway Antigen Sampling System: Respiratory M Cells as an Alternative Gateway for Inhaled Antigens Peer-reviewed

    Dong-Young Kim, Ayuko Sato, Satoshi Fukuyama, Hiroshi Sagara, Takahiro Nagatake, Il Gyu Kong, Kaoru Goda, Tomonori Nochi, Jun Kunisawa, Shintaro Sato, Yoshifumi Yokota, Chul Hee Lee, Hiroshi Kiyono

    The Journal of Immunology　186　(7)　4253-4262　2011/04/01

    Publisher: The American Association of Immunologists

    DOI： 10.4049/jimmunol.0903794 　

    ISSN： 0022-1767

    eISSN： 1550-6606

  83. Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress Peer-reviewed

    Kazutaka Terahara, Tomonori Nochi, Masato Yoshida, Yuko Takahashi, Yoshiyuki Goto, Hirotsugu Hatai, Shiho Kurokawa, Myoung Ho Jang, Mi-Na Kweon, Steven E. Domino, Takachika Hiroi, Yoshikazu Yuki, Yasuko Tsunetsugu-Yokota, Kazuo Kobayashi, Hiroshi Kiyono

    Biochemical and Biophysical Research Communications　404　(3)　822-828　2011/01/21

    DOI： 10.1016/j.bbrc.2010.12.067 　

    ISSN： 0006-291X 1090-2104

  84. In vivo molecular imaging analysis of a Nasal vaccine that induces protective immunity against botulism in nonhuman primates Peer-reviewed

    Yoshikazu Yuki, Tomonori Nochi, Norihiro Harada, Yuko Katakai, Hiroaki Shibata, Mio Mejima, Tomoko Kohda, Daisuke Tokuhara, Shiho Kurokawa, Yuko Takahashi, Fumiko Ono, Shunji Kozaki, Keiji Terao, Hideo Tsukada, Hiroshi Kiyono

    Journal of Immunology　185　(9)　5436-5443　2010/11/01

    Publisher: The American Association of Immunologists

    DOI： 10.4049/jimmunol.1001789 　

    ISSN： 0022-1767 1550-6606

    eISSN： 1550-6606

  85. Antigen-sampling cells in the salmonid intestinal epithelium Peer-reviewed

    Bjørn Fuglem, Emilio Jirillo, Inge Bjerkås, Hiroshi Kiyono, Tomonori Nochi, Yoshikazu Yuki, Martin Raida, Uwe Fischer, Erling Olaf Koppang

    Developmental and Comparative Immunology　34　(7)　768-774　2010/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.dci.2010.02.007 　

    ISSN： 0145-305X

  86. Secretory IgA-mediated protection against V. cholerae and heat-labile enterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine Peer-reviewed

    Daisuke Tokuhara, Yoshikazu Yuki, Tomonori Nochi, Toshio Kodama, Mio Mejima, Shiho Kurokawa, Yuko Takahashi, Masanobu Nanno, Ushio Nakanishi, Fumio Takaiwa, Takeshi Honda, Hiroshi Kiyono

    Proceedings of the National Academy of Sciences of the United States of America　107　(19)　8794-8799　2010/05/11

    Publisher: Proceedings of the National Academy of Sciences

    DOI： 10.1073/pnas.0914121107 　

    ISSN： 0027-8424 1091-6490

    eISSN： 1091-6490

  87. Indigenous opportunistic bacteria inhabit mammalian gut-associated lymphoid tissues and share a mucosal antibody-mediated symbiosis Peer-reviewed

    Takashi Obata, Yoshiyuki Goto, Jun Kunisaw, Shintaro Sato, Mitsuo Sakamoto, Hiromi Setoyama, Takahiro Matsuki, Kazuhiko Nonaka, Naoko Shibata, Masashi Gohda, Yuki Kagiyama, Tomonori Nochi, Yoshikazu Yuki, Yoshiko Fukuyama, Akira Mukai, Shinichiro Shinzaki, Kohtaro Fujihashi, Chihiro Sasakawa, Hideki Iijima, Masatoshi Goto, Yoshinori Umesaki, Yoshimi Benno, Hiroshi Kiyono

    Proceedings of the National Academy of Sciences of the United States of America　107　(16)　7419-7424　2010/04/20

    Publisher: Proceedings of the National Academy of Sciences

    DOI： 10.1073/pnas.1001061107 　

    ISSN： 0027-8424 1091-6490

    eISSN： 1091-6490

  88. Localization of fatty acid binding protein of epidermal type common to dendritic cells and presumptive macrophages in Peyer's patches and epithelial M cells of mouse intestine Peer-reviewed

    Ryoji Suzuki, Mohammad Reza Nourani, Sachiko Saino-Saito, Hiroshi Abe, Tomonori Nochi, Hiroshi Kiyono, Friedrich Spener, Hisatake Kondo, Yuji Owada

    Histochemistry and Cell Biology　132　(6)　577-584　2009/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00418-009-0638-5 　

    ISSN： 0948-6143

    eISSN： 1432-119X

  89. A rice-based oral cholera vaccine induces macaque-specific systemic neutralizing antibodies but does not influence pre-existing intestinal immunity Peer-reviewed

    Tomonori Nochi, Yoshikazu Yuki, Yuko Katakai, Hiroaki Shibata, Daisuke Tokuhara, Mio Mejima, Shiho Kurokawa, Yuko Takahashi, Ushio Nakanishi, Fumiko Ono, Hitomi Mimuro, Chihiro Sasakawa, Fumio Takaiwa, Keiji Terao, Hiroshi Kiyono

    Journal of Immunology　183　(10)　6538-6544　2009/11/15

    Publisher: The American Association of Immunologists

    DOI： 10.4049/jimmunol.0901480 　

    ISSN： 0022-1767 1550-6606

    eISSN： 1550-6606

  90. RANKL is necessary and sufficient to initiate development of antigen-sampling M cells in the intestinal epithelium Peer-reviewed

    Kathryn A. Knoop, Nachiket Kumar, Betsy R. Butler, Senthilkumar K. Sakthivel, Rebekah T. Taylor, Tomonori Nochi, Hisaya Akiba, Hideo Yagita, Hiroshi Kiyono, Ifor R. Williams

    Journal of Immunology　183　(9)　5738-5747　2009/11/01

    Publisher: The American Association of Immunologists

    DOI： 10.4049/jimmunol.0901563 　

    ISSN： 0022-1767 1550-6606

    eISSN： 1550-6606

  91. Id2-, RORγt-, and LTβR-independent initiation of lymphoid organogenesis in ocular immunity Peer-reviewed

    Takahiro Nagatake, Satoshi Fukuyama, Dong-Young Kim, Kaoru Goda, Osamu Igarashi, Shintaro Sato, Tomonori Nochi, Hiroshi Sagara, Yoshifumi Yokota, Anton M. Jetten, Tsuneyasu Kaisho, Shizuo Akira, Hitomi Mimuro, Chihiro Sasakawa, Yoshinori Fukui, Kohtaro Fujihashi, Taishin Akiyama, Jun-Ichiro Inoue, Josef M. Penninger, Jun Kunisawa, Hiroshi Kiyono

    Journal of Experimental Medicine　206　(11)　2351-2364　2009/10/26

    Publisher: Rockefeller University Press

    DOI： 10.1084/jem.20091436 　

    ISSN： 0022-1007 1540-9538

    eISSN： 1540-9538

  92. Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity Peer-reviewed

    Yoshikazu Yuki, Daisuke Tokuhara, Tomonori Nochi, Hiroshi Yasuda, Mio Mejima, Shiho Kurokawa, Yuko Takahashi, Nobuhiro Kataoka, Ushio Nakanishi, Yukari Hagiwara, Kohtaro Fujihashi, Fumio Takaiwa, Hiroshi Kiyono

    Vaccine　27　(43)　5982-5988　2009/10/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.vaccine.2009.07.071 　

    ISSN： 0264-410X

  93. Expression of newly identified secretory CEACAM1a isoforms in the intestinal epithelium Peer-reviewed

    Kazutaka Terahara, Masato Yoshida, Fumihiro Taguchi, Osamu Igarashi, Tomonori Nochi, Yoshiyuki Gotoh, Takuya Yamamoto, Yasuko Tsunetsugu-Yokota, Nicole Beauchemin, Hiroshi Kiyono

    Biochemical and Biophysical Research Communications　383　(3)　340-346　2009/06/05

    Publisher: Elsevier BV

    DOI： 10.1016/j.bbrc.2009.04.008 　

    ISSN： 0006-291X 1090-2104

  94. New horizon of mucosal immunity and vaccines Peer-reviewed

    Ichiro Takahashi, Tomonori Nochi, Yoshikazu Yuki, Hiroshi Kiyono

    Current Opinion in Immunology　21　(3)　352-358　2009/06

    Publisher: Elsevier BV

    DOI： 10.1016/j.coi.2009.04.002 　

    ISSN： 0952-7915

  95. Immunological commonalities and distinctions between airway and digestive immunity Peer-reviewed

    Jun Kunisawa, Tomonori Nochi, Hiroshi Kiyono

    Trends in Immunology　29　(11)　505-513　2008/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.it.2008.07.008 　

    ISSN： 1471-4906

  96. A soluble nonglycosylated recombinant infectious hematopoietic necrosis virus (IHNV) G-protein induces IFNs in rainbow trout (Oncorhynchus mykiss) Peer-reviewed

    Noel Verjan, Ei Lin Ooi, Tomonori Nochi, Hidehiro Kondo, Ikuo Hirono, Takashi Aoki, Hiroshi Kiyono, Yoshikazu Yuki

    Fish and Shellfish Immunology　25　(1-2)　170-180　2008/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.fsi.2008.04.004 　

    ISSN： 1050-4648 1095-9947

  97. Biological characterisation of a recombinant Atlantic salmon type I interferon synthesized in Escherichia coli Peer-reviewed

    Ei Lin Ooi, Noel Verjan, Ikuo Hirono, Tomonori Nochi, Hidehiro Kondo, Takashi Aoki, Hiroshi Kiyono, Yoshikazu Yuki

    Fish and Shellfish Immunology　24　(5)　506-513　2008/05

    Publisher: Elsevier BV

    DOI： 10.1016/j.fsi.2007.10.004 　

    ISSN： 1050-4648 1095-9947

  98. Peyer's patches are required for intestinal immunoglobulin A responses to salmonella spp. Peer-reviewed

    Tomomi Hashizume, Atsushi Togawa, Tomonori Nochi, Osamu Igarashi, Mi-Na Kweon, Hiroshi Kiyono, Masafumi Yamamoto

    Infection and Immunity　76　(3)　927-934　2008/03

    Publisher: American Society for Microbiology

    DOI： 10.1128/IAI.01145-07 　

    ISSN： 0019-9567

    eISSN： 1098-5522

  99. Comprehensive gene expression profiling of peyer's patch m cells, villous m-like cells, and intestinal epithelial cells Peer-reviewed

    Kazutaka Terahara, Masato Yoshida, Osamu Igarashi, Tomonori Nochi, Gemilson Soares Pontes, Koji Hase, Hiroshi Ohno, Shiho Kurokawa, Mio Mejima, Naoko Takayama, Yoshikazu Yuki, Anson W. Lowe, Hiroshi Kiyono

    Journal of Immunology　180　(12)　7840-7846　2008

    Publisher: American Association of Immunologists

    DOI： 10.4049/jimmunol.180.12.7840 　

    ISSN： 1550-6606 0022-1767

    eISSN： 1550-6606

  100. Progress towards an AIDS mucosal vaccine: An overview Peer-reviewed

    Yoshikazu Yuki, Tomonori Nochi, Hiroshi Kiyono

    Tuberculosis　87　(1)　S35-S44　2007/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.tube.2007.05.005 　

    ISSN： 1472-9792

  101. Establishment of a Poliovirus Oral Infection System in Human Poliovirus Receptor-Expressing Transgenic Mice That Are Deficient in Alpha/Beta Interferon Receptor Peer-reviewed

    Seii Ohka, Hiroko Igarashi, Noriyo Nagata, Mai Sakai, Satoshi Koike, Tomonori Nochi, Hiroshi Kiyono, Akio Nomoto

    Journal of Virology　81　(15)　7902-7912　2007/08/01

    Publisher: American Society for Microbiology

    DOI： 10.1128/jvi.02675-06 　

    ISSN： 0022-538X

    eISSN： 1098-5514

    More details Close

    <title>ABSTRACT</title> Poliovirus (PV) is easily transferred to humans orally; however, no rodent model for oral infections has been developed because of the alimentary tract's low sensitivity to the virus. Here we showed that PV is inactivated by the low pH of the gastric contents in mice. The addition of 3% NaHCO3 to the viral inoculum increased the titer of virus reaching the small intestine through the stomach after intragastric inoculation of PV. Transgenic mice (Tg) carrying the human PV receptor (hPVR/CD155) gene and lacking the alpha/beta interferon receptor (IFNAR) gene (hPVR-Tg/<italic>Ifnar</italic>KO) were sensitive to the oral administration of PV with 3% NaHCO3, whereas hPVR-Tg expressing IFNAR were much less sensitive. The virus was detected in the epithelia of the small intestine and proliferated in the alimentary tract of hPVR-Tg/<italic>Ifnar</italic>KO. By the ninth day after the administration of a virulent PV, the mice had died. These results suggest that IFNAR plays an important role in determining permissivity in the alimentary tract as well as the generation of virus-specific immune responses to PV via the oral route. Thus, hPVR-Tg/<italic>Ifnar</italic>KO are considered to be the first oral infection model for PV, although levels of anti-PV antibodies were not elevated dramatically in serum and intestinal secretions of surviving mice when hPVR-Tg/<italic>Ifnar</italic>KO were administered an attenuated PV.

  102. Role of Peyer's patches in the induction of Helicobacter pylori-induced gastritis Peer-reviewed

    Shigenori Nagai, Hitomi Mimuro, Taketo Yamada, Yukiko Baba, Kazuyo Moro, Tomonori Nochi, Hiroshi Kiyono, Toshihiko Suzuki, Chihiro Sasakawa, Shigeo Koyasu

    Proceedings of the National Academy of Sciences of the United States of America　104　(21)　8971-8976　2007/05/22

    Publisher: Proceedings of the National Academy of Sciences

    DOI： 10.1073/pnas.0609014104 　

    ISSN： 0027-8424

    eISSN： 1091-6490

  103. Innate immunity in the mucosal immune system Peer-reviewed

    Tomonori Nochi, Hiroshi Kiyono

    Current Pharmaceutical Design　12　(32)　4203-4213　2006/11

    Publisher: Bentham Science Publishers Ltd.

    DOI： 10.2174/138161206778743457 　

    ISSN： 1381-6128

  104. Localization of interleukin-18 and its receptor in somatotrophs of the bovine anterior pituitary gland Peer-reviewed

    Yasuhiro Nagai, Tomonori Nochi, Kouichi Watanabe, Keisuke Watanabe, Hisashi Aso, Haruki Kitazawa, Masatoshi Matsuzaki, Shyuichi Ohwada, Takahiro Yamaguchi

    Cell and Tissue Research　322　(3)　455-462　2005/12

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00441-005-0016-0 　

    ISSN： 0302-766X

    eISSN： 1432-0878

  105. Linkage between innate and acquired immunities at the mucosa Peer-reviewed

    Osamu Igarashi, Tomonori Nochi, Kazutaka Terahara, Hiroshi Kiyono

    International Congress Series　1285　84-93　2005/11

    DOI： 10.1016/j.ics.2005.08.017 　

    ISSN： 0531-5131

  106. Biological role of Ep-CAM in the physical interaction between epithelial cells and lymphocytes in intestinal epithelium Peer-reviewed

    Tomonori Nochi, Yoshikazu Yuki, Kazutaka Terahara, Ayako Hino, Jun Kunisawa, Mi-Na Kweon, Takahiro Yamaguchi, Hiroshi Kiyono

    Clinical Immunology　113　(3)　326-339　2004/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.clim.2004.08.013 　

    ISSN： 1521-6616

  107. Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium Peer-reviewed

    Mayumi Ueta, Tomonori Nochi, Myoung-Ho Jang, Eun Jeong Park, Osamu Igarashi, Ayako Hino, Satoshi Kawasaki, Takashi Shikina, Takachika Hiroi, Shigeru Kinoshita, Hiroshi Kiyono

    Journal of Immunology　173　(5)　3337-3347　2004/09/01

    Publisher: American Association of Immunologists

    DOI： 10.4049/jimmunol.173.5.3337 　

    ISSN： 0022-1767

    eISSN： 1550-6606

  108. Intestinal villous M cells: An antigen entry site in the mucosal epithelium Peer-reviewed

    Myoung Ho Jang, Mi-Na Kweon, Koichi Iwatani, Masafumi Yamamoto, Kazutaka Terahara, Chihiro Sasakawa, Toshihiko Suzuki, Tomonori Nochi, Yoshifumi Yokota, Paul D. Rennert, Takachika Hiroi, Hiroshi Tamagawa, Hideki Iijima, Jun Kunisawa, Yoshikazu Yuki, Hiroshi Kiyono

    Proceedings of the National Academy of Sciences of the United States of America　101　(16)　6110-6115　2004/04/20

    Publisher: Proceedings of the National Academy of Sciences

    DOI： 10.1073/pnas.0400969101 　

    ISSN： 0027-8424

    eISSN： 1091-6490

  109. Neutrophil proteinase 3-mediated induction of bioactive IL-18 secretion by human oral epithelial cells Peer-reviewed

    S. Sugawara, A. Uehara, T. Nochi, T. Yamaguchi, H. Ueda, A. Sugiyama, K. Hanzawa, K. Kumagai, H. Okamura, H. Takada

    Journal of Immunology　167　(11)　6568-6575　2001/12/01

    Publisher: American Association of Immunologists

    DOI： 10.4049/jimmunol.167.11.6568 　

    ISSN： 0022-1767

    eISSN： 1550-6606

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    野地智法

    臨床獣医　51-53　2020/07

  12. 臨床獣医師のためのBrush Up免疫学（第18回）乳汁免疫が発達するメカニズムとは？

    野地智法

    臨床獣医　58-60　2020/05

  13. 臨床獣医師のためのBrush Up免疫学（第17回）遅延型過敏症に関する理解を深めよう！

    野地智法

    臨床獣医　75-77　2020/03

  14. 臨床獣医師のためのBrush Up免疫学（第16回）アレルギーを発症するメカニズムを理解しよう！

    野地智法

    臨床獣医　50-53　2020/01

  15. 臨床獣医師のためのBrush Up免疫学（第15回）ワクチンを適切に使用するために知っておくべきこと

    野地智法

    臨床獣医　38-41　2019/11

  16. 臨床獣医師のためのBrush Up免疫学（第14回）ワクチンが効くメカニズムとは

    野地智法

    臨床獣医　51-53　2019/09

  17. 臨床獣医師のためのBrush Up免疫学（第13回）細胞が死ぬメカニズムと免疫系との関わりとは？

    野地智法

    臨床獣医　51-53　2019/07

  18. 臨床獣医師のためのBrush Up免疫学（第12回）サイトカインについての理解を深めよう

    野地智法

    臨床獣医　55-57　2019/05

  19. 臨床獣医師のためのBrush Up免疫学（第11回）Th1とTh2についての理解を深めよう

    野地智法

    臨床獣医　62-64　2019/03

  20. 臨床獣医師のためのBrush Up免疫学（第10回）細胞障害性T細胞とは？

    野地智法

    臨床獣医　74-76　2019/01

  21. 食餌性トリプトファンが消化管におけるIgA産生に及ぼす影響

    Islam Jahidul, 白川 仁, 駒井 三千夫, 宇佐美 克紀, 野地 智法, 麻生 久

    無菌生物　48　(2)　84-85　2018/12

    Publisher: 日本無菌生物ノートバイオロジー学会

    ISSN： 0910-0903

  22. 臨床獣医師のためのBrush Up免疫学（第9回）T細胞について理解しよう

    野地智法

    臨床獣医　74-76　2018/11

  23. 臨床獣医師のためのBrush Up免疫学（第8回）抗体のアイソタイプとは？

    野地智法

    臨床獣医　62-64　2018/09

  24. 臨床獣医師のためのBrush Up免疫学（第7回）Ｂ細胞について理解しよう

    野地智法

    臨床獣医　61-63　2018/07

  25. 臨床獣医師のためのBrush Up免疫学(第6回) 免疫はいつ発達する?

    野地 智法

    臨床獣医　36　(5)　54-56　2018/05

    Publisher: (株)緑書房

    ISSN： 0912-1501

  26. 臨床獣医師のためのBrush Up免疫学(第5回) 一次リンパ組織と二次リンパ組織

    野地 智法

    臨床獣医　36　(3)　55-57　2018/03

    Publisher: (株)緑書房

    ISSN： 0912-1501

  27. 臨床獣医師のためのBrush Up免疫学(第4回) ノーベル賞研究に近づこう!

    野地 智法

    臨床獣医　36　(1)　49-51　2018/01

    Publisher: (株)緑書房

    ISSN： 0912-1501

  28. LPS-binding proteinはリポ多糖によるCD14依存的Toll様受容体4内在化を媒介し、TRIFシグナルを制御する

    塚本 宏樹, 武内 偲乃, 久保田 佳苗, 小坂井 沙緒, 紫竹 歩, 鵜飼 一歩, 小林 洋平, 大久保 美咲, 沼崎 宗夫, 金光 祥臣, 松本 洋太郎, 野地 智法, 渡邊 康一, 麻生 久, 富岡 佳久

    生命科学系学会合同年次大会　2017年度　[2LBA-013]　2017/12

    Publisher: 生命科学系学会合同年次大会運営事務局

  29. 黄色ブドウ球菌性乳房炎における乳汁中乳腺上皮細胞の割合は乳腺組織の損傷を反映する

    長澤 裕哉, 菊 佳男, 菅原 和恵, 田邉 扶由子, 石川 義春, 市居 修, 野地 智法, 麻生 久, 昆 泰寛, 門田 耕一, 林 智人

    家畜感染症学会誌　6　(4)　165-165　2017/12

    Publisher: 家畜感染症学会誌編集委員会

    ISSN： 2186-7208

  30. LPS‐binding proteinはリポ多糖によるCD14依存的Toll様受容体4内在化を媒介しTRIFシグナルを制御する

    塚本宏樹, 武内偲乃, 久保田佳苗, 小坂井沙緒, 紫竹歩, 鵜飼一歩, 小林洋平, 大久保美咲, 沼崎宗夫, 金光祥臣, 松本洋太郎, 野地智法, 渡邊康一, 麻生久, 富岡佳久

    日本生化学会大会(Web)　90th　ROMBUNNO.2LBA‐013 (WEB ONLY)-013]　2017/12

    Publisher: 生命科学系学会合同年次大会運営事務局

  31. 臨床獣医師のためのBrush Up免疫学(第3回) 補体について知ろう

    野地 智法

    臨床獣医　35　(12)　40-42　2017/11

    Publisher: (株)緑書房

    ISSN： 0912-1501

  32. 臨床獣医師のためのBrush Up免疫学(第2回) 自然免疫について少しだけ学ぼう

    野地 智法

    臨床獣医　35　(10)　54-56　2017/09

    Publisher: (株)緑書房

    ISSN： 0912-1501

  33. 二次リンパ組織の形態形成に関するブタ胎仔を用いた組織学的研究

    古川 睦実, 伊藤 駿, 盛田 彰太郎, 鈴木 俊一, 淵本 大一郎, 中井 美智子, 大西 彰, 新實 香奈枝, 宇佐美 克紀, 佐々木 志保, 渡邊 康一, 麻生 久, 野地 智法

    日本獣医学会学術集会講演要旨集　160回　309-309　2017/08

    Publisher: (公社)日本獣医学会

    ISSN： 1347-8621

  34. X-SCIDモデルブタ(IL2Rg欠損ブタ)の各種臓器内に認められるT細胞およびB細胞分布に関する組織学的研究

    伊藤 駿, 古川 睦実, 盛田 彰太郎, 鈴木 俊一, 淵本 大一郎, 中井 美智子, 大西 彰, 新實 香奈枝, 宇佐美 克紀, 佐々木 志保, 渡邊 康一, 麻生 久, 野地 智法

    日本獣医学会学術集会講演要旨集　160回　373-373　2017/08

    Publisher: (公社)日本獣医学会

    ISSN： 1347-8621

  35. マウス腸管上皮M細胞におけるアルドラーゼA受容体の探索

    鈴木京, 長澤裕哉, 田中孝太朗, 庄涛, 田口輝明, 北澤春樹, 渡邊康一, 野地智法, 麻生久

    日本畜産学会大会講演要旨　122nd　176　2017/03/28

    ISSN： 1342-4688

  36. 熱ストレスを負荷したニワトリにおける解糖減少は免疫応答と関連している(Decreased Glycolysis Relates to Immune Response in Heat-Stressed Chicken)

    Nurjanah Siti, 野地 智法, 南都 文香, 古川 恭平, 阿部 汐里, 福田 栞, 喜久里 基, 豊水 正昭

    日本畜産学会大会講演要旨集　122回　127-127　2017/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  37. 黄色ブドウ球菌特異的IgG抗体はSortase A非依存的に細胞増殖を阻害する(S. aureus-specific IgG antibodies inhibit the bacterial growth in a Sortase A independent manner)

    古川 睦実, 米山 裕, 秦 英司, 安藤 太助, 林 智人, 菊 佳男, 長澤 裕哉, 渡邊 康一, 麻生 久, 野地 智法

    日本細菌学雑誌　72　(1)　65-65　2017/02

    Publisher: 日本細菌学会

    ISSN： 0021-4930

    eISSN： 1882-4110

  38. 黄色ブドウ球菌性乳房炎における乳汁中乳腺上皮細胞の割合は乳腺組織の損傷を反映する

    長澤 裕哉, 菊 佳男, 菅原 和恵, 田邉 扶由子, 石川 義春, 市居 修, 野地 智法, 麻生 久, 昆 泰寛, 門田 耕一, 林 智人

    家畜感染症学会誌　5　(4)　180-180　2016/12

    Publisher: 家畜感染症学会誌編集委員会

    ISSN： 2186-7208

  39. 黄色ブドウ球菌(SA)の死菌接種によって産生されるポリクローナル抗体はSAに対する増殖阻害効果を有する

    古川 睦実, 米山 裕, 秦 英司, 岩野 英知, 樋口 豪紀, 安藤 太助, 佐藤 美佳, 林 智人, 菊 佳男, 長澤 裕哉, 新實 香奈枝, 宇佐美 克紀, 佐々木 志保, 伊藤 駿, 渡邊 康一, 麻生 久, 野地 智法

    家畜感染症学会誌　5　(4)　181-181　2016/12

    Publisher: 家畜感染症学会誌編集委員会

    ISSN： 2186-7208

  40. 豚マイコプラズマ性肺炎抗病性育種豚の肺抗病性機能に対するワクチンの影響

    佐久間晶子, 佐久間晶子, 吉野淳良, 高橋伸和, 氏家哲, 陸拾七, 鈴木啓一, 須田義人, 渡邉康一, 野地智法, 麻生久

    日本養豚学会大会講演要旨　105th　2　2016/11/10

  41. Comparison of innate immune related molecules and an entrail environment between landrace pigs that have been selected for genetic resistance to mycoplasmal pneumonia of swine (MPS) and normal landrace pigs

    佐久間晶子, 吉野淳良, 高橋伸和, 野地智法, 須田義人, 麻生久

    食肉に関する助成研究調査成果報告書　34　266‐271　2016/11

  42. フローサイトメトリーを用いた授乳期の乳腺に認められるIgA産生形質細胞の性状解析

    宇佐美克紀, 新實香奈枝, 古川睦実, 藤田勇気, 阿部未来, 渡邊康一, 麻生久, 野地智法

    日本獣医学会学術集会講演要旨集　159th　362　2016/08/30

    ISSN： 1347-8621

  43. 褐色脂肪細胞の活性化による経口セロトニンの抗肥満作用

    田口輝明, 日高湧介, 海野剛, 對木陽淳, 陳香凝, 北澤春樹, 渡邊康一, 野地智法, 麻生久

    東北畜産学会報　66　(2)　25　2016/08/12

    ISSN： 1341-626X

  44. 枯草菌給与によるウシ分娩前後における血中樹状細胞の機能解析

    庄涛, 浦川めぐみ, 佐藤秀俊, 高田直和, 及川俊徳, 小林宏子, 伊藤愛, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    東北畜産学会報　66　(2)　26　2016/08/12

    ISSN： 1341-626X

  45. 黄色ブドウ球菌のin vitroでの増殖阻害を可能にするウシポリクローナル抗体の作製

    古川睦実, 米山裕, 秦英司, 岩野英知, 樋口豪紀, 安藤太助, 佐藤美佳, 林智人, 菊佳男, 長澤裕哉, 新實香奈枝, 宇佐美克紀, 佐々木志保, 伊藤駿, 渡邊康一, 麻生久, 野地智法

    東北畜産学会報　66　(2)　27　2016/08/12

    ISSN： 1341-626X

  46. ウシ咽頭扁桃は鼻腔投与した黄色ブドウ球菌死菌に対する免疫応答の場になる

    長澤裕哉, 菊佳男, 菅原和恵, 田邉扶由子, 石川義春, 野地智法, 市居修, 昆泰寛, 麻生久, 門田耕一, 林智人

    日本獣医学会学術集会講演要旨集　159回　362-362　2016/08

    Publisher: (公社)日本獣医学会

    ISSN： 1347-8621

  47. 基礎と臨床で理解を深めよう!乳房炎Q and A Question.16 なぜ乳房炎対策に下痢症予防ワクチンを使うのか?

    野地智法, 森本和秀

    臨床獣医　34　(7)　6‐8　2016/07/01

    ISSN： 0912-1501

  48. 乳汁に分泌される白血球遊走因子シクロフィリンAを指標とした乳房炎診断法への応用

    高梨暁, 浦川めぐみ, 鈴木京, 田口輝明, 菊佳男, 林智人, 佐藤秀俊, 渡邊康一, 渡邊康一, 野地智法, 野地智法, 麻生久, 麻生久

    日本畜産学会大会講演要旨　121st　197-197　2016/03/27

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  49. 乳腺を標的としたIgA誘導型粘膜ワクチン開発を目指した学術基盤の形成

    新實香奈枝, 阿部未来, 藤田勇気, 盛田彰太郎, 古川睦実, 宇佐美克紀, 朴恩正, 佐藤慎太郎, 清野宏, 渡邊康一, 麻生久, 野地智法, 野地智法

    日本畜産学会大会講演要旨　121st　148　2016/03/27

    ISSN： 1342-4688

  50. 乳汁中に黄色ブドウ球菌特異的IgA抗体を誘導させる鼻腔を介した抗原取込み機序の解析

    長澤裕哉, 菊佳男, 野地智法, 麻生久, 田邉扶由子, 菅原和恵, 石川義春, 門田耕一, 林智人

    日本獣医学会学術集会講演要旨集　158th　270-270　2015/08/30

    Publisher: (公社)日本獣医学会

    ISSN： 1347-8621

  51. 品種交雑が選抜ランドレース種豚の抗病性関連遺伝子に及ぼす影響

    高橋光, 菅原静夏, 長澤裕哉, 寺田俊介, 板谷奈波, 佐藤貴俊, 藤田勇気, 北澤春樹, 島津朋之, 鈴木啓一, 野地智法, 渡邊康一, 麻生久

    日本畜産学会大会講演要旨　119th　187　2015/03/28

    ISSN： 1342-4688

  52. トランスポゾン挿入変異法を用いたウシ乳房炎関連病原因子の解明に向けた基盤研究

    佐藤美佳, 勝部哲, 野地智法, 林智人, 秦英司, 菊佳男, 安藤太助, 麻生久, 磯貝恵美子, 米山裕

    日本畜産学会大会講演要旨　119th　115-115　2015/03/28

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  53. 乳房炎誘導牛における乳汁中シクロフィリンA濃度の定量分析と乳房炎早期発見への応用

    梅村紗緒里, 高梨暁, 板谷奈波, 浦川めぐみ, 菊佳男, 林智人, 渡邉康一, 野地智法, 麻生久

    日本畜産学会大会講演要旨　119th　115-115　2015/03/28

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  54. 乳房炎と乳汁中シクロフィリン分泌量の関連性

    梅村紗緒里, 高梨暁, 板谷奈波, 佐藤勝祥, 浦川めぐみ, 菊佳男, 林智人, 渡邊康一, 野地智法, 麻生久

    日本乳房炎研究会第19回学術集会proceedings　17-20　2015/03

  55. 枯草菌給与による乳房炎発症予防効果の検討

    佐藤勝祥, 佐藤秀俊, 浦川めぐみ, 板谷奈波, 高梨暁, 梅村紗緒里, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    日本乳房炎研究会第19回学術集会proceedings　49-52　2015/03

  56. 枯草菌給与が乳牛の血中免疫担当細胞へ与える影響

    浦川めぐみ, 佐藤勝祥, 佐藤秀俊, 板谷奈波, 高梨暁, 梅村紗緒里, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    日本乳房炎研究会第19回学術集会proceedings　43-56　2015/03

  57. 枯草菌給与に夜乳房炎発症予防効果とその作用機構

    麻生久, 佐藤秀俊, 浦川めぐみ, 佐藤勝祥, 板谷奈波, 高梨暁, 梅村紗緒里, 芦田延久, 今林寛和, 渡邊康一, 野地智法

    ルーメン研究会報　26　(1)　17-22　2015

  58. Self-assembled polysaccharide nanogels for nasal delivery of biopharmaceuticals

    Tomonori Nochi, Yoshikazu Yuki, Kazunari Akiyoshi, Hiroshi Kiyono

    Mucosal Delivery of Biopharmaceuticals: Biology, Challenges and Strategies　9781461495246　325-332　2014/10/01

    Publisher: Springer US

    DOI： 10.1007/978-1-4614-9524-6_13 　

  59. 乳汁中シクロフィリン測定による乳房炎発症リスクの定量的分析

    梅村紗緒里, 高梨暁, 板谷奈波, 佐藤勝祥, 浦川めぐみ, 菊佳男, 林智人, 渡邊康一, 野地智法, 麻生久

    東北畜産学会報　64　(2)　27-27　2014/08/26

    Publisher: 東北畜産学会

    ISSN： 1341-626X

  60. 新生仔ブタ腸管に認められる初乳取り込み能を持つ上皮細胞の組織学的研究

    盛田彰太郎, 長澤裕哉, 渡邊康一, 野地智法, 麻生久

    日本獣医学会学術集会講演要旨集　157th　321　2014/08/11

    ISSN： 1347-8621

  61. ウシ腸管M細胞における解糖系酵素アルドラーゼAの合成と分泌

    長澤裕哉, 盛田彰太郎, 日高湧介, 鈴木京, 渡邉一史, 渡邊康一, 大和田修一, 野地智法, 北澤春樹, 今村守一, 横山隆, 堀内基広, 坂口末廣, 麻生久

    日本獣医学会学術集会講演要旨集　157th　321　2014/08/11

    ISSN： 1347-8621

  62. IgA産生細胞を効果的に乳腺組織へ遊走させるための免疫戦略の確立

    藤田勇紀, 阿部未来, 秦英司, 菊佳男, 林智人, 渡邊康一, 野地智法, 麻生久

    日本獣医学会学術集会講演要旨集　157th　386-386　2014/08/11

    Publisher: (公社)日本獣医学会

    ISSN： 1347-8621

  63. セロトニン腹腔内投与が高脂肪飼料摂取マウスの腸管セロトニン分泌細胞に与える影響

    住吉敬介, 渡邉一史, 齋藤遼, 中野辰也, 長澤裕哉, 陳香凝, 岡田夏実, 渡邊康一, 野地智法, 麻生久

    日本畜産学会大会講演要旨　118th　148　2014/03/27

    ISSN： 1342-4688

  64. 抗病性選抜ランドレース豚の免疫特性解析

    管原静夏, 高橋光, 長澤裕哉, 寺田俊介, 白須直樹, 住吉敬介, 板谷奈波, 北澤春樹, 島津朋之, 鈴木啓一, 野地智法, 渡邊康一, 麻生久

    日本畜産学会大会講演要旨　118th　154　2014/03/27

    ISSN： 1342-4688

  65. マウス褐色脂肪細胞株の樹立

    陳香凝, 長澤裕哉, 渡邉一史, 齋藤遼, 住吉敬介, 岡田夏美, 佐藤勝祥, 渡邊康一, 野地智法, 麻生久

    日本畜産学会大会講演要旨　118th　155　2014/03/27

    ISSN： 1342-4688

  66. 枯草菌給与によるウシ乳房炎発症予防と血中栄養因子およびストレス指標の変動

    佐藤勝祥, 浦川めぐみ, 板谷奈波, 佐藤秀俊, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    日本乳房炎研究会第18回学術集会Proceedings　71-74　2014/03

  67. マウス腸管M細胞における解糖系酵素アルドラーゼAを介した異常型プリオン蛋白質取り込み機構

    長澤裕哉, 盛田彰太郎, 日高湧介, 渡邉一史, 大和田修一, 北澤春樹, 今村守一, 横山隆, 堀内基広, 坂口末廣, 西田教行, 渡邊康一, 野地智法, 麻生久

    日本獣医学会学術集会講演要旨集　156th　196　2013/08/30

    ISSN： 1347-8621

  68. Recent progress for the development of mucosal vaccine

    野地 智法, 清野 宏

    Protein, nucleic acid and enzyme　54　(8)　1089-1095　2009/06/01

    Publisher: 共立出版

    ISSN： 0039-9450

  69. Development of M cell-targeted oral vaccine for effective activation of gastrointestinal immune system

    野地 智法, 清野 宏

    Clinical immunology & allergology　51　(1)　97-104　2009/01

    Publisher: 科学評論社

    ISSN： 1881-1930

  70. コメ型経口ワクチンによる粘膜感染症の予防法の確立を目指して

    野地 智法, 幸 義和, 清野 宏

    ファルマシア　44　(7)　689-692　2008/07/01

    Publisher: 公益社団法人日本薬学会

    ISSN： 0014-8601

  71. Oral rice-based non-toxic mutant cholera toxin induces enterotoxin neutralizing immunity

    Yoshikazu Yuki, Daisuke Tokuhara, Tomonori Nochi, Hiroshi Yasuda, Mio Mejima, Shiho Kurokawa, Fumio Takaiwa, Hiroshi Kiyono

    FASEB JOURNAL　22　2008/04

    ISSN： 0892-6638

  72. Influence of commensal bacteria on the induction of UEA-1(+) NKM-16-2-4(+) cells in small intestine

    Yoshiyuki Gotoh, Takashi Obata, Osamu Igarashi, Kazutaka Terahara, Tomonori Nochi, Jun Kunisawa, Shintaro Satoh, Mitsuo Sakamoto, Takahiro Matsuki, Yoshinori Umesaki, Yoshimi Benno, Hiroshi Kiyono

    FASEB JOURNAL　22　2008/04

    ISSN： 0892-6638

  73. A subunit type of botulinum mucosal vaccine effectively induces protective immunity in non-human primates

    Tomonori Nochi, Yoshikazu Yuki, Mio Mejima, Fumiko Ono, Yuko Katakai, Hiroaki Shibata, Tomoko Kohda, Shunji Kozaki, Keiji Terao, Hiroshi Kiyono

    FASEB JOURNAL　22　2008/04

    ISSN： 0892-6638

  74. Clinical impact of cholera toxin B subunit expressed rice-based vaccine(MucoRice): Crucial protective role of antigen specific secretory IgA and cross-protection against cholera toxin and E.coli heat labile enterotoxin

    D. Tokuhara, Y. Yuki, T. Nochi, M. Mejima, S. Kurokawa, H. Kiyono

    VACCINE　2008

    ISSN： 0264-410X

  75. Exploit of nanogel as a novel carrier of mucosal vaccine: Nanogel mucosal vaccine effectively induces protective immunity against botulinum toxin

    Tomonori Nochi, Yoshikazu Yuki, Mio Mejima, Nobuhiro Kataoka, Kazunari Akiyoshi, Hiroshi Kiyono

    VACCINE　2008

    ISSN： 0264-410X

  76. Development of effective protein delivery system to mucosa-associated lymphoid tissues (MALT) with M cell-targeting technology

    Tomonori Nochi, Hiroshi Kiyono

    Drug Delivery System　23　(5)　529-533　2008

    Publisher: THE JAPAN SOCIETY OF DRUG DELIVERY SYSTEM

    DOI： 10.2745/dds.23.529 　

    ISSN： 1881-2732 0913-5006

  77. Rice-based mucosal vaccine

    徳原 大介, 野地 智法, 幸 義和

    Virus report　4　(2)　94-99　2007/11

    Publisher: 医薬ジャーナル社

    ISSN： 1349-6956

  78. Mucosal vaccine in the near future

    野地 智法, 清野 宏

    医学のあゆみ　221　(11)　917-920　2007/06/16

    Publisher: 医歯薬出版

    ISSN： 0039-2359

  79. 免疫系のしくみとその制御 腸管粘膜免疫の特殊性 (生涯教育シリーズ(67)わかりやすい免疫疾患) -- (免疫の基礎)

    野地 智法, 清野 宏

    日本医師会雑誌　134　(0)　S43-45　2005/06

    Publisher: 日本医師会

    ISSN： 0021-4493

  80. Mucosal immunology

    Tomonori Nochi, Hiroshi Kiyono

    Nippon rinsho. Japanese journal of clinical medicine　63 Suppl 4　14-19　2005/01/01

    ISSN： 0047-1852

  81. 乳腺の粘膜免疫機構の解析(1) 泌乳期牛乳腺組織部位でのT細胞サブセットの選択的分布

    黒石 智誠, 野地 智法, 浅井 健一, 山口 高弘, 熊谷 勝男

    日本免疫学会総会・学術集会記録　30　207-207　2000/11

    Publisher: (NPO)日本免疫学会

    ISSN： 0919-1984

Show all ︎Show first 5

Books and Other Publications 1

  1. Humanized Mice for HIV Research

    Denton PW, Nochi T, Garcia JV

    Springer　2014

Presentations 190

  1. Strategies to Increase Antibody Levels in the Milk of Lactating Animals

    Tomonori Nochi

    UC Davis Seminar　2025/08/08

  2. 母乳由来の免疫因子が子の腸管における細菌叢形成に及ぼす影響 Invited

    野地智法

    日本乳酸菌学会2025年度大会　2025/07/05

  3. 粘膜免疫の視座から再考する乳腺の免疫機能 Invited

    野地智法

    BMR研修会　2025/06/28

  4. 母子移行型IgAの機能解明と次世代の健全育成を目的としたその活用 Invited

    野地智法

    第2回IgA抗体医療学会　2025/06/25

  5. 子牛の下痢症治療を目的とした有用微生物叢移植の有効性と作用機序 Invited

    野地智法

    令和６年度長崎県獣医師会産業動物臨床部会講習会　2025/03/21

  6. 農学領域から見える粘膜免疫学研究の魅力 Invited

    野地智法

    千葉大学医学部 cSIMVaセミナー　2025/02/04

  7. 母乳免疫の機能形成に関わる分子・細胞メカニズム Invited

    野地智法

    日本無菌生物ノートバイオロジー学会　2025/01/24

  8. 粘膜免疫学研究の可能性 Invited

    野地智法

    仙台ARTクリニックセミナー　2024/12/20

  9. 家畜の生産性向上を目的とした粘膜免疫学研究の最前線 Invited

    野地智法

    大阪公立大学セミナー　2024/12/14

  10. The origin and role of B cells in the avian gut-associated lymphoid tissues Invited

    Tomonori Nochi

    Wageningen University & Research Seminars　2024/12/04

  11. Gut-mammary gland axis for producing maternal IgA in breast milk Invited

    Tomonori Nochi

    Paul-Ehrlich-Institut Special Seminar　2024/12/02

  12. Research on the microbiota for the treatment of diarrhea in calves Invited

    Tomonori Nochi

    BRIN Summer Seminar 2024　2024/11/05

  13. Development of fecal microbiota transplantation for calf diarrhea treatment Invited

    Tomonori Nochi

    The 11th International Conference of Innovation in Animal Science (ICIAS)　2024/11/04

  14. Cross fecal microbiota transplantation to treat diarrhea in pre-weaned calves Invited

    Tomonori Nochi

    1st International Seminar on Food Science and Technology 2024 (1st IsoFST 2024)　2024/10/30

  15. Fecal microbiota transplantation to improve microbiota for curing diarrhea in calves Invited

    Tomonori Nochi

    VI International Symposium on Lactic Acid Bacteria, Argentinean-Japanese Lactic Acid Bacteria “Tohoku Forum for Creativity” Meeting　2024/08/08

  16. 子牛の下痢症制御を目的とした糞便移植研究の最前線 Invited

    野地智法

    JRA日本中央競馬会「令和６年度畜産振興事業」宮崎大学　2024/07/29

  17. 腸内微生物叢改善による子牛の難治性下痢症の改善 Invited

    野地智法

    第38回中国しゃくなげ会　2024/07/18

  18. Recent advances in milk science based on the knowledge of immunology and microbiology Invited

    Tomonori Nochi

    2023/11/20

  19. 農学領域における粘膜免疫学研究から見えてきた母体の免疫機能のユニーク性 Invited

    野地智法

    第19回日本食品免疫学会　2023/11/14

  20. 持続可能な畜産を追求した有用微生物の活用と総合知によるその応用展望 Invited

    野地智法

    SOKAPキックオフシンポジウム　2023/11/06

  21. 乳腺粘膜免疫の最前線 ～母乳抗体の由来とは～ Invited

    野地智法

    第２回臨床粘膜免疫研究会　2023/10/28

  22. 幼若家畜の腸管に備わる免疫微生物環境を質向上させる意義を問う Invited

    野地智法

    第１回動物生産におけるDOHaDと免疫および遺伝的背景の関係シンポジウム　2023/09/21

  23. 腸内の微生物環境によって制御される免疫機能に関する包括的理解とその賦活化を目的とした応用研究 Invited

    野地智法

    第50回日本毒性学会　2023/06/19

  24. The Gut Microbiota Induces Peyer's-Patch-Dependent Secretion of Maternal IgA into Milk Invited

    Tomonori Nochi

    Gordon Research Conference 2023 Mammary Gland Biology　2023/05/31

  25. 糞便移植の実用化を目指した基礎と臨床の融合研究の最前線 Invited

    野地智法

    NOSAI沖縄講習会　2022/11/22

  26. 乳腺免疫ダイナミクス～母から子に受け継がれる乳汁免疫の仕組み～ Invited

    野地智法

    第９回日本DOHaD学会　2022/10/08

  27. 子牛の下痢症制御を目的とした糞便移植の有効性とその作用機序 Invited

    野地智法

    北獣オホーツク支部新技術講習会　2022/08/26

  28. 腸内細菌が宿主免疫系に与える影響 ～腸内微生物ーパイエル板ー乳腺経路の発見～ Invited

    野地智法

    第55回日本無菌生物ノートバイオロジー学会　2022/01/21

  29. 家畜の生産性向上を目指した粘膜免疫学研究の最前線 Invited

    野地智法

    第156回鹿児島県家畜臨床研究会　2021/11/27

  30. X連鎖重症複合免疫不全症に対する胎生期治療の有用性 ～医療用免疫不全ブタを用いた動物モデル研究から見えてきたこと～ Invited

    野地智法

    第12回関東甲越免疫不全症研究会　2021/09/19

  31. 粘膜免疫学研究の最前線 ～粘膜ワクチン開発に向けた基礎研究から見えてきたこと～ Invited

    野地智法

    大動物臨床研究会 第１０回東京シンポジウム　2021/02/27

  32. 腸管免疫ダイナミクス～臓器間をつなぐ腸管に秘められた免疫の力～ Invited

    野地智法

    第10回家畜感染症学会　2020/12/12

  33. 乳腺免疫研究から見えてきたこと～哺乳動物の繁殖に欠かせない母体の免疫戦略～ Invited

    野地智法

    第113回日本繁殖生物学会　2020/09/25

  34. 免疫不全ブタを実験動物とした医農連携研究の最前線 Invited

    野地智法

    第67回日本実験動物学会（紙上開催）　2020/05/23

  35. 乳腺の機能形成に関わる免疫微生物ダイナミクス Invited

    野地智法

    第26回中四国乳房炎協議会　2018/12/22

  36. 乳房炎研究の最先端～ここまで分かった乳腺免疫～ Invited

    野地智法

    第49回日本家畜臨床学会　2018/11/15

  37. 乳腺免疫のユニーク性と乳房炎予防に向けた新概念の構築 Invited

    野地智法

    第22回日本乳房炎研究会　2017/10/06

  38. 経鼻ワクチン開発の最前線～粘膜組織で効果的なIgA産生を可能にするナノゲルの威力～ Invited

    野地智法

    動物用ワクチン－バイオ医薬品研究会 2016年シンポジウム　2016/09/08

  39. ウシ乳腺免疫系の発達を促す分子メカニズムとは Invited

    野地智法

    東北乳房炎研究協議会 第3回研究集会　2016/03/05

  40. 乳腺でのIgA産生を促す分子メカニズムとは Invited

    野地智法

    中四国乳房炎協議会 第14回勉強会　2015/12/19

  41. 農免疫研究を基盤とした経口ワクチン開発 Invited

    野地智法

    第5回共同開催フォーラム 食科学の近未来-守りと攻めの備えは万全か-　2015/12/04

  42. 粘膜免疫学に立脚した粘膜感染症に対する予防・治療技術開発の重要性 Invited

    野地智法

    農研機構動物衛生研究所北海道支所 平成27年度集談会　2015/11/17

  43. ニワトリ卵管へ向けたIgA産生細胞の遊走に雌性ホルモンが与える影響の解析

    前川紗佳子, 平川良太, 久松基史, 浅井映輝, Jahidul Islam, 古川睦実, 野地智法

    日本家禽学会 2025春季大会

  44. ファブリキウス嚢依存的・非依存的に分泌される腸管IgA抗体の腸内細菌叢制御機構の解明

    平川良太, 久松基史, 前川紗佳子, 浅井映輝, Islam Jahidul, 古川睦実, 野地智法

    日本家禽学会 2025春季大会

  45. Discovery of Bursa-independent B cell differentiation in gastrointestinal tracts of chickens

    Ryota Hirakawa, Motoshi Hisamatsu, Sayoko Maekawa, Eiki Asai, Jahidul Islam, Mutsumi Furukawa, Tomonori Nochi

    The International Congress of Mucosal Immunology (ICMI) 2024

  46. Extensive dissemination and efficacy evaluation of selected donor feces for practical application of fecal microbiota transplantation in livestock

    Tomonori Nochi, Jahidul Islam, Natsuki Ohtani, Yu Shimizu, Junya Yamazaki, Zen Nakamura, Hidekazu Tanaka

    The International Congress of Mucosal Immunology (ICMI) 2024

  47. Formation of the junctions between lymph follicles in the Peyer's patches even before postweaning activation

    Anri Teshigahara, Hiromi Yoshida, Mitsuji Kaji, Ryota Hirakawa, Jahidul Islam, Mutsumi Furukawa, Tomonori Nochi

    The International Congress of Mucosal Immunology (ICMI) 2024

  48. Therapeutic efficacy of freeze-dried microbiota transplantation for calf diarrhea

    Natsuki Ohtani, Yu Shimizu, Jahidul Islam, Hidekazu Tanaka, Tomonori Nochi

    32nd World Buiatrics Congress

  49. Establishing cross-FMT intervention for calf diarrhea treatment in pre-weaned calves

    Jahidul Islam, Natsuki Ohtani, Junya Yamazaki, Zen Nakamura, Hidekazu Tanaka, Tomonori Nochi

    32nd World Buiatrics Congress

  50. 研究大学における未来志向の教育・研究者育成を目的としたプログラムの開発とその実践 Invited

    野地智法

    早稲田大学大学総合研究センター 第5回 Faculty Café　2024/01/19

  51. Milk-derived immune factors affect intestinal immune development in infant

    2024/01/19

  52. MEL-Bが有する黄色ブドウ球菌に対する抗菌活性に関わる遺伝子の同定

    山内清哉, 下田 蒼, 菅原知宏, 山本周平, 曽我部敦, 古川睦実, 米山 裕, 野地智法

    第28回日本乳房炎研究会　2023/10/21

  53. 性成熟前後のニワトリ卵管におけるIgA産生能の評価

    前川紗佳子, 平川良太, 久松基史, 山下司, 古川睦実, 野地智法

    日本家禽学会2023年度秋季大会　2023/09/21

  54. 3D可視化技術を用いたニワトリ盲腸扁桃の形態形成機序の解明

    久松基史, 平川良太, 前川紗佳子, 古川睦実, 野地智法

    第131回日本畜産学会　2023/09/19

  55. シングルセル解析を用いた乳腺由来細胞の遺伝子発現プロファイリング

    和田詩野, Jahidul Islam, 内野紗江佳, 山下司, 平川良太, 古川睦実, 野地智法

    第7回乳房炎サマーキャンプ　2023/09/17

  56. 乳汁IgAの標的微生物の同定と子の腸内微生物叢形成機構の解明

    小山紗江佳, 山下司, 和田誌野, 平川良太, Jahidul Islam, 古川睦実, 野地智法

    第7回乳房炎サマーキャンプ　2023/09/17

  57. 離乳後の乳腺に存在するIgA産生長命型形質細胞の機能解明

    山下司, 小山紗江佳, 和田詩野, 平川良太, Jahidul Islam, 古川睦実, 野地智法

    第7回乳房炎サマーキャンプ　2023/09/17

  58. ニワトリ腸管に備わるファブリキウス嚢非依存的なB細胞分化経路の特定

    平川良太, 久松基史, 前川紗佳子, 宇佐美克紀, 岡田拓歩, Jahidul Islam, 古川睦実, 野地智法

    第166回日本獣医学会学術集会

  59. Detection of S-IgA antibodies in plasma of neonates and identification of the source

    伊東加織、櫻井光太、古川睦実、野地智法

    第51回日本免疫学会

  60. 幼若期のパイエル板形成を理解するための3D可視化技術を用いた機能形態学的解析

    勅使河原杏莉, 小山真, 酒井義文, 吉田裕美, 鍛冶光司, 佐藤慎太郎, 古川睦実, 野地智法

    第130回日本畜産学会

  61. Elucidation of the efficacy of fecal microbiome transplantation (FMT) in healing calves with intractable diarrhea

    Jahidul Islam, Yu Shimizu, Masae Ishii, Yoshiaki Gotou, Eriko Tatezaki, Masumi Satou, Eiji Makino, Touru Shimada, Chise Ueda, Natsuki Ohtani, Mutsumi Furukawa, Hisashi Aso, Hidekazu Tanaka, Tomonori Nochi

    World Buiatrics Congress 2022

  62. Identification of potential microbial taxa involved in the efficacy of fecal microbiota transplantation (FMT) from healthy donors to recipients suffering from diarrhea in calves

    Natsuki Ohtani, Jahidul Islam, Yu Shimizu, Masae Ishii, Yoshiaki Gotou, Eriko Tatezaki, Masumi Satou, Eiji Makino, Touru Shimada, Chise Ueda, Mutsumi Furukawa, Hisashi Aso, Hidekazu Tanaka, Tomonori Nochi

    World Buiatrics Congress 2022

  63. ニワトリ腸管に発達する免疫臓器の機能解明に資する組織学的研究

    平川良太, 久松基史, 古川睦実, 佐藤幹, 野地智法

    日本家禽学会2021年度秋季大会　2021/09/16

  64. 乳汁IgAの産生制御因子としてのPolymeric immunoglobulin Receptor（PigR）の機構解明

    伊東加織, 内野紗江佳, 宇佐美克紀, 古川睦実, 松本敬, 南野昌信, 麻生久, 野地智法

    第129回日本畜産学会　2021/09/14

  65. 3D可視化技術を用いた幼若期のパイエル板の形態学的解析

    勅使河原杏莉, 古川睦実, 野地智法

    第70回東北畜産学会　2021/09/06

  66. 乳汁IgAが有する腸内微生物に対する特異性評価

    内野紗江佳, 宇佐美克紀, 新實香奈枝, 古川睦実, 野地智法

    第70回東北畜産学会　2021/09/06

  67. バイオサーファクタント（MEL-B）の有する脂質依存的な黄色ブドウ球菌増殖抑制効果

    山内清哉, 山本周平, 菅原知宏, 曽我部敦, 秦英司, 古川睦実, 米山裕, 渡邊康一, 麻生久, 野地智法

    第163回日本獣医学会

  68. 初乳中自然免疫因子Serum Amyloid Aの同定とその作用標的

    内野紗江佳, 宇佐美克紀, 佐々木志保, 新實香奈枝, 古川睦実, 渡邊康一, 麻生久, 野地智法

    第163回日本獣医学会

  69. 出生直後のブタ回腸パイエル板の形態形成および機能成熟に関する組織学的研究

    古川睦実, 伊藤駿, 鈴木俊一, 淵本大一郎, 大西彰, 渡邊康一, 麻生久, 野地智法

    第163回日本獣医学会

  70. A role of Peyer’s patches in establishing immunological and microbial environments in the gastrointestinal tract just after weaning

    Niimi K, Usami K, Aso H, Nochi T

    第48回日本免疫学会　2019/12/12

  71. A role of intestinal microorganisms in inducing maternal IgA production

    Usami K, Niimi K, Aso H, Nochi T

    第48回日本免疫学会　2019/12/12

  72. 黒毛和種哺乳子牛におけるビタミンCの投与効果

    乙丸孝之介, 大石祥子, 藤村裕, 岩本悠紀, 永井克尚, 窪田力, 山内清哉, 野地智法

    第9回家畜感染症学会　2019/12/07

  73. 放牧と自給粗飼料で飼養するDM日本短角種における哺乳期の増体特性

    小笠原英毅, 石井絵梨, 野原香菜, 高橋辰行, 渡邊康一, 野地智法, 麻生久, 寳示戸雅之

    第126回日本畜産学会　2019/09/18

  74. 離乳直後の腸内の免疫・微生物環境形成に及ぼすパイエル板の役割

    新實香奈枝, 宇佐美克紀, 古川睦実, 馬場柚里, 山内清哉, 渡邊康一, 麻生久, 野地智法

    第126回日本畜産学会　2019/09/18

  75. 炎症誘起因子シクロフィリンAの乳房炎誘起能の発見

    遠藤佑真, 佐藤佑子, 小堤知之, 浅野貴史, 熊谷弘明, 吉村梢, 庄涛, 浦川めぐみ, 渡邊康一, 野地智法, 麻生久

    第126回日本畜産学会　2019/09/17

  76. 乳汁中のIgA移行抗体産生を促す腸内微生物の生物学的意義

    宇佐美克紀, 新實香奈枝, 古川睦実, 馬場柚里, 内野紗江佳, 山内清哉, 渡邊康一, 麻生久, 野地智法

    第162回日本獣医学会　2019/09/11

  77. 乳房炎牛の乳牛中で増加するシクロフィリンAは乳房炎を誘起する

    遠藤佑真, 吉村梢, 熊谷弘明, 浅野貴史, 小堤知之, 庄涛, 浦川めぐみ, 渡邊康一, 野地智法, 麻生久

    第6回乳房炎サマーキャンプ　2019/09/04

  78. 乳腺で機能する自然免疫関連因子の同定とその発現解析

    内野紗江佳, 宇佐美克紀, 佐々木志保, 新實香奈枝, 古川睦実, 渡邊康一, 麻生久, 野地智法

    第6回乳房炎サマーキャンプ　2019/09/04

  79. 肉質等級の異なる黒毛和種牛における筋線維型構成および細胞外マトリクス成分の局在様式

    矢倉彩乃, 石川澄華, 渡邊康一, 野地智法, 麻生久

    第69回東北畜産学会　2019/08/27

  80. 枯草菌を給与した初産牛は炎症状態に陥りにくく健康な状態を維持する

    吉村梢, 浦川めぐみ, 遠藤佑真, 桂哲平, 浅野貴史, 熊谷弘明, 日野正浩, 庄涛, 渡邊康一, 野地智法, 麻生久

    第69回東北畜産学会　2019/08/27

  81. 暑熱環境下のニワトリにおける液性免疫機能の低下機序の解明

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    第125回日本畜産学会　2019/03/29

  82. Microarray analysis of bovine mammary epithelial cells stimulated with mastitis pathogens

    高木理宏, 小松稜弥, Julio Villena、Leonardo Albarracin, Md. Aminul Islam, 須田義人, 大坪和香子, 野地智法, 麻生久, 北澤春樹

    第125回日本畜産学会　2019/03/29

  83. IL-2Rg欠損が免疫・微生物・代謝系に与える影響とX-SCIDブタへの胎生期造血幹細胞移植の有用性評価

    伊藤駿, 鈴木俊一, 淵本大一郎, 大西彰, 板野理, 北郷実, 松田祐子, 盛田彰太郎, 古川睦実, 新實香奈枝, 宇佐美克紀, 渡邊康一, 麻生久, 野地智法

    第125回日本畜産学会　2019/03/29

  84. Identification of the origin and specificity of maternal IgA antibodies produced in the mammary gland

    宇佐美克紀, 新實香奈枝, 佐々木志保, 古川睦実, 伊藤駿, 馬場柚里, 山内清哉, 渡邊康一, 麻生久, 野地智法

    第125回日本畜産学会　2019/03/29

  85. The disturbance of maternal microbial environment affects the intestinal immune development in offspring

    Niimi K, Usami K, Aso H, Nochi T

    第47回日本免疫学会　2018/12/10

  86. The role of Peyer’s patches in producing maternal IgA antibodies

    Usami K, Niimi K, Aso H, Nochi T

    第47回日本免疫学会　2018/12/10

  87. ３D可視化技術を用いたパイエル板の濾胞・傍濾胞領域の形態学的解析

    馬場柚里, 古川睦実, 新實香奈枝, 宇佐美克紀, 佐々木志保, 伊藤駿, 渡邊康一, 麻生久, 野地智法

    第161回日本獣医学会　2018/09/11

  88. ウシ乳房炎誘起因子シクロフィリンAの乳汁における存在様式

    吉村梢, 海野剛, 浦川めぐみ, 庄涛, 田中孝太朗, 遠藤佑真, 佐藤佑子, 小堤知行, 佐藤秀俊, 渡邊康一, 野地智法, 麻生久

    第161回日本獣医学会　2018/09/11

  89. パイエル板から乳腺に遊走するIgA産生細胞の同定

    宇佐美克紀, 新實香奈枝, 佐々木志保, 馬場柚里, 古川睦実, 伊藤駿, 渡邊康一, 麻生久, 野地智法

    第161回日本獣医学会　2018/09/11

  90. Elucidation of immunological and metabolic alterations associated with heat-stress in chickens

    Hirakawa R, Nochi T, Furukawa K, Kikusato M, Toyomizu M

    2018 Fall Meeting of Japan Poultry Science Association International Symposium　2018/09/06

  91. 暑熱条件下で抗原接種した鶏の液性免疫応答が遅延する原因解明

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    第124回日本家禽学会　2018/09/05

  92. 乳中の炎症誘起因子シクロフィリンAの存在様式と乳房炎発症との関連性

    吉村梢, 海野剛, 浦川めぐみ, 庄涛, 田中孝太朗, 遠藤佑真, 佐藤佑子, 小堤知行, 佐藤秀俊, 渡邊康一, 野地智法, 麻生久

    第5回乳房炎サマーキャンプ　2018/09/04

  93. パイエル板から乳腺へのIgA産生細胞遊走の実証

    宇佐美克紀, 新實香奈枝, 古川睦実, 佐々木志保, 伊藤駿, 馬場柚里, 渡邊康一, 麻生久, 野地智法

    第5回乳房炎サマーキャンプ　2018/09/04

  94. ファージディスプレイ法による乳房炎由来黄色ブドウ球菌特異的ペプチドの探索

    岩松隼矢, 米山竜太, 古川睦実, 野地智法, 松田敬一, 安藤太助, 米山裕

    第5回乳房炎サマーキャンプ　2018/09/04

  95. 抗菌剤による母体の腸内細菌叢攪乱がもたらす母乳を介した仔への影響評価

    新實香奈枝, 宇佐美克紀, 佐々木志保, 古川睦実, 伊藤駿, 馬場柚里, 渡邊康一, 麻生久, 野地智法

    第68回東北畜産学会　2018/08/30

  96. シクロフィリンAの乳頭投与が乳汁中体細胞数に与える影響

    佐藤佑子, 吉村梢, 浦川めぐみ, 庄涛, 田中孝太朗, 遠藤佑真, 伊藤愛, 小堤知行, 小林宏子, 高田直和, 渡邊康一, 野地智法, 麻生久

    第68回東北畜産学会　2018/08/30

  97. Oral feeding with a probiotics Bacillus subtilis C-3102 modulates the immune and metabolic functions in dairy cattle, resulting in prevention of mastitis

    Urakawa M, Sato H, Zhuang T, Taguchi T, Katto S, Tanaka K, Kobayashi H, Ito M, Sato Y, Ashida N, Otomo N, Watanabe K, Nochi T, Aso H

    The 30th World Buiatrics Congress　2018/08/28

  98. Effect of probiotic Bacillus Subtilis C-3102 strain on function of dendritic cells in bovine peripheral blood before and after parturition

    Zhuang T, Urakawa M, Sato H, Kobayashi H, Sato Y, Ashida N, Otomo N, Watanabe K, Nochi T, Aso H

    The 30th World Buiatrics Congress　2018/08/28

  99. CCL28-mediated chemotaxis for the recruitment of IgA-producing plasma cells into the mammary gland depends on the lactation-related but not bacterial stimulus

    Nochi T, Niimi K, Usami K, Furukawa M, Sasaki S, Kiyono H, Watanabe K, Aso H

    Mucosal Immunology Course & Symposium (MICS2018)　2018/07/19

  100. Intestinal microflora changes during lactation and has relevance to the specificity of milk IgA and diversity of microflora in the mammary gland

    Niimi K, Usami K, Furukawa M, Sasaki S, Watanabe K, Aso H, Nochi T

    Mucosal Immunology Course & Symposium (MICS2018)　2018/07/19

  101. Effects of oleuropein on lipoplysaccharide-induced pepatic injury in mice

    Otani A, Shirakawa H, Nochi T, Toyomizu M, Kikusato M

    第124回日本畜産学会　2018/03/28

  102. 暑熱環境が肉用鶏のリンパ組織に及ぼす影響

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    第124回日本畜産学会　2018/03/28

  103. 末梢セロトニンの褐色脂肪細胞活性化による抗肥満作用

    田口輝明, 甲藤士郎, 田中孝太朗, 庄涛, 吉村梢, 渡邊康一, 野地智法, 麻生久

    第124回日本畜産学会　2018/03/28

  104. ブタにおける二次リンパ組織の形態形成機序の解明

    古川睦実, 伊藤駿, 鈴木俊一, 淵本大一郎, 渡邊康一, 麻生久, 野地智法

    第124回日本畜産学会　2018/03/28

  105. 初産牛における乳汁中の体細胞数と炎症誘起因子シクロフィリンA濃度との相関解析

    海野剛, 浦川めぐみ, 庄涛, 田中孝太朗, 吉村梢, 佐藤佑子, 伊藤愛, 小林宏子, 渡邊康一, 野地智法, 麻生久

    第124回日本畜産学会　2018/03/28

  106. Important role of the immunological network between large intestine and mammary gland in breastfeeding

    Usami K, Niimi K, Kamioka M, Kiyono H, Aso H, Nochi T

    第46回日本免疫学会　2017/12/12

  107. Development of immune and microbial environments is independently regulated in the mammary gland

    Niimi K, Usami K, Aso H, Nochi T

    第46回日本免疫学会　2017/12/12

  108. プロバイオティクス枯草菌によるウシ乳房炎の発症軽減効果

    浦川めぐみ, 佐藤秀俊, 庄涛, 田口輝明, 海野剛, 田中孝太朗, 甲藤士郎, 吉村梢, 小林宏子, 伊藤愛, 高田直和, 芦田延久, 大友直樹, 渡邊康一, 野地智法, 麻生久

    第22回日本乳房炎研究会　2017/10/06

  109. X-SCIDモデルブタ（IL2Rg欠損ブタ）の各種臓器内に認められるＴ細胞およびＢ細胞分布に関する組織学的研究

    伊藤駿, 古川睦実, 盛田彰太郎, 鈴木俊一, 淵本大一郎, 中井美智子, 大西彰, 新實香奈枝, 宇佐美克紀, 佐々木志保, 渡邊康一, 麻生久, 野地智法

    第160回日本獣医学会　2017/09/13

  110. 二次リンパ組織の形態形成に関するブタ胎仔を用いた組織学的研究

    古川睦実, 伊藤駿, 盛田彰太郎, 鈴木俊一, 淵本大一郎, 中井美智子, 大西彰, 新實香奈枝, 宇佐美克紀, 佐々木志保, 渡邊康一, 麻生久, 野地智法

    第160回日本獣医学会　2017/09/13

  111. Dispensable role of indigenous bacteria in developing the mammary gland functions

    Niimi K, Usami K, Furukawa M, Sasaki S, Ito S, Banba Y, Watanabe K, Aso H, Nochi T

    第123回日本畜産学会　2017/09/06

  112. 末梢セロトニンの骨格筋線維型移行および代謝亢進作用の解明

    甲藤士郎, 田口輝明, 田中孝太朗, 庄涛, 吉村梢, 渡邊康一, 野地智法, 麻生久

    第123回日本畜産学会　2017/09/06

  113. 乳腺と大腸をつなぐ新規免疫誘導経路の解明

    宇佐美克紀, 新實香奈枝, 古川睦実, 佐々木志保, 伊藤駿, 馬場柚里, 渡邊康一, 麻生久, 野地智法

    第4回乳房炎サマーキャンプ　2017/08/26

  114. 乳腺の機能形成に与える卵巣由来物質の影響とは

    佐々木志保, 新實香奈枝, 宇佐美克紀, 古川睦実, 伊藤駿, 馬場柚里, 渡邊康一, 麻生久, 野地智法

    第4回乳房炎サマーキャンプ　2017/08/26

  115. 授乳期の乳腺免疫発達における卵巣の機能的役割

    佐々木志保, 新實香奈枝, 宇佐美克紀, 古川睦実, 伊藤駿, 馬場柚里, 渡邊康一, 麻生久, 野地智法

    第67回東北畜産学会　2017/08/18

  116. 乳腺と大腸をつなぐ免疫ネットワークの解明

    宇佐美克紀, 新實香奈枝, 古川睦実, 佐々木志保, 伊藤駿, 渡邊康一, 麻生久, 野地智法

    第67回東北畜産学会　2017/08/18

  117. 乳腺と大腸をつなぐ免疫ネットワークの解明

    宇佐美克紀, 新實香奈枝, 古川睦実, 佐々木志保, 伊藤駿, 渡邊康一, 麻生久, 野地智法

    第122回日本畜産学会　2017/03/27

  118. ウシ血中樹状細胞の精製法確立と枯草菌給与による活性化機構の解明

    庄涛, 浦川めぐみ, 鈴木京, 田口輝明, 佐藤秀俊, 高田直和, 及川俊徳, 小林宏子, 伊藤愛, 芦田延久, 小林寛和, 渡邊康一, 野地智法, 麻生久

    第122回日本畜産学会　2017/03/27

  119. マウス腸管上皮M 細胞におけるアルドラーゼA受容体の探索

    鈴木京, 長澤裕哉, 田中孝太朗, 庄涛, 田口輝明, 北澤春樹, 渡邊康一, 野地智法, 麻生久

    第122回日本畜産学会　2017/03/27

  120. 枯草菌の給与が乳牛の血中栄養因子および免疫因子に与える影響

    浦川めぐみ, 佐藤秀俊, 庄涛, 鈴木京, 田口輝明, 小林宏子, 伊藤愛, 高田直和, 及川俊徳, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第122回日本畜産学会　2017/03/27

  121. Decreased Glycolysis Relates to Immune Response in Heat-Stressed Chicken

    Nurjanah Siti, 野地智法, 南都文香, 古川恭平, 阿部汐里, 福田栞, 喜久里基, 豊水正昭

    第122回日本畜産学会　2017/03/27

  122. 黄色ブドウ球菌特異的IgG抗体はSortase A非依存的に細胞増殖を阻害する

    古川睦実, 米山裕, 秦英司, 安藤太助, 林智人, 菊佳男, 長澤裕哉, 渡邊康一, 麻生久, 野地智法

    第90回日本細菌学会　2017/03/19

  123. 乳腺での感染防御を可能にする免疫賦活化因子の探索

    古川睦実, 野地智法

    平成28年度東京大学医科学研究所共同利用・共同研究拠点事業成果報告会　2017/03/16

  124. 黄色ブドウ球菌性乳房炎における乳汁中乳腺上皮細胞の割合は乳腺組織の損傷を反映する

    長澤裕哉, 菊佳男, 菅原和恵, 田邉扶由子, 石川義春, 市居修, 野地智法, 麻生久, 昆泰寛, 門田耕一, 林智人

    第6回家畜感染症学会　2016/12/02

  125. 黄色ブドウ球菌（SA）の死菌接種によって産生されるポリクローナル抗体はSAに対する増殖阻害効果を有する

    古川睦実, 米山裕, 秦英司, 岩野英知, 樋口豪紀, 安藤太助, 佐藤美佳, 林智人, 菊佳男, 長澤裕哉, 新實香奈枝, 宇佐美克紀, 佐々木志保, 伊藤駿, 渡邊康一, 麻生久, 野地智法

    第6回家畜感染症学会　2016/12/02

  126. 枯草菌のウシ乳房炎発症抑制効果と血中樹状細胞活性化機能の関係

    庄涛, 浦川めぐみ, 佐藤秀俊, 高田直和, 及川俊徳, 小林宏子, 伊藤愛, 芦田延久, 小林寛和, 渡邊康一, 野地智法, 麻生久

    第21回日本乳房炎研究会　2016/10/07

  127. ウシ咽頭扁桃は鼻腔投与した黄色ブドウ球菌死菌に対する免疫応答の場になる

    長澤裕哉, 菊佳男, 菅原和恵, 田邉扶由子, 石川義春, 野地智法, 市居修, 昆泰寛, 麻生久, 門田耕一, 林智人

    第159回日本獣医学会　2016/09/06

  128. フローサイトメトリーを用いた授乳期の乳腺に認められるIgA産生形質細胞の性状解析

    宇佐美克紀, 新實香奈枝, 古川睦実, 藤田勇気, 阿部未来, 渡邊康一, 麻生久, 野地智法

    第159回日本獣医学会　2016/09/06

  129. 褐色脂肪細胞の活性化による経口セロトニンの抗肥満作用

    田口輝明, 日高湧介, 海野剛, 對木陽淳, 陳香凝, 北澤春樹, 渡邊康一, 野地智法, 麻生久

    第66回東北畜産学会　2016/09/06

  130. 枯草菌給与によるウシ分娩前後における血中樹状細胞の機能解析

    庄涛, 浦川めぐみ, 佐藤秀俊, 高田直和, 及川俊徳, 小林宏子, 伊藤愛, 芦田延久, 今林寛和, 小林寛和, 渡邊康一, 野地智法, 麻生久

    第66回東北畜産学会　2016/09/06

  131. 黄色ブドウ球菌のin vitroでの増殖阻害を可能にするウシポリクローナル抗体の作製

    古川睦実, 米山裕, 秦英司, 岩野英知, 樋口豪紀, 安藤太助, 佐藤美佳, 林智人, 菊佳男, 長澤裕哉, 新實香奈枝, 宇佐美克紀, 佐々木志保, 伊藤駿, 渡邊康一, 麻生久, 野地智法

    第66回東北畜産学会　2016/09/06

  132. 黄色ブドウ球菌（SA）特異的ウシポリクローナル抗体を用いたSA増殖阻害効果の検討

    古川睦実, 米山裕, 秦英司, 岩野英知, 樋口豪紀, 安藤太助, 佐藤美佳, 林智人, 菊佳男, 長澤裕哉, 新實香奈枝, 宇佐美克紀, 佐々木志保, 伊藤駿, 渡邊康一, 麻生久, 野地智法

    第3回乳房炎サマーキャンプ　2016/09/03

  133. 授乳期の乳腺でのIgA産生を促す要因とは（その１）

    宇佐美克紀, 新實香奈枝, 古川睦実, 藤田勇気, 阿部未来, 佐々木志保, 伊藤駿, 陶山佳久, 酒井義文, 渡邊康一, 麻生久, 野地智法

    第3回乳房炎サマーキャンプ　2016/09/03

  134. 授乳期の乳腺でのIgA産生を促す要因とは（その２）

    新實香奈枝, 宇佐美克紀, 古川睦実, 藤田勇気, 阿部未来, 佐々木志保, 伊藤駿, 渡邊康一, 麻生久, 野地智法

    第3回乳房炎サマーキャンプ　2016/09/03

  135. 枯草菌の給与が乳牛の乳房炎予防および健康状態に与える影響

    浦川めぐみ, 庄涛, 鈴木京, 田口輝明, 佐藤秀俊, 小林宏子, 伊藤愛, 及川俊徳, 高田直和, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第3回乳房炎サマーキャンプ　2016/09/03

  136. Fat metabolic capacity of ruminant-specific type I myofiber subtype. International-presentation

    Youjun Tsuiki, Takatoshi Sato, Tomonori Nochi, Hisashi Aso, Kouichi Watanabe

    17th AAAP Animal Science Congress 2016　2016/08/23

  137. 乳腺を標的としたIgA誘導型粘膜ワクチン開発を目指した学術基盤の形成

    新實香奈枝, 阿部未来, 藤田勇気, 盛田彰太郎, 古川睦実, 宇佐美克紀, 朴恩正, 佐藤慎太郎, 清野宏, 渡邊康一, 麻生久, 野地智法

    第121回日本畜産学会　2016/03/27

  138. ウシID型筋線維の脂肪代謝能に関する免疫組織化学的解析

    對木陽淳, 佐藤貴俊, 野地智法, 麻生久, 渡邊康一

    第121回日本畜産学会　2016/03/27

  139. 乳汁に分泌される白血球遊走因子シクロフィリンAを標的とした乳房炎診断法への応用

    高梨暁, 浦川めぐみ, 鈴木京, 田口輝明, 菊佳男, 林智人, 佐藤秀俊, 渡邊康一, 野地智法, 麻生久

    第121回日本畜産学会　2016/03/27

  140. 乳房炎多発牛への枯草菌給与と血中免疫担当細胞の動態

    浦川めぐみ, 高梨暁, 佐藤秀俊, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第121回日本畜産学会　2016/03/27

  141. 経口投与したセロトニンの抗肥満作用と骨格筋エネルギー代謝に与える影響

    日高湧介, 寺田俊介, 佐藤勝祥, 海野剛, 田口輝明, 陳香凝, 浦川めぐみ, 渡邊康一, 野地智法, 麻生久

    第121回日本畜産学会　2016/03/27

  142. 免疫不全ブタを用いたパイエル板の機能及び形態形成に関する免疫学的研究

    盛田彰太郎, 鈴木俊一, 渡邊康一, 麻生久, 大西彰, 野地智法

    第121回日本畜産学会　2016/03/27

  143. 乳腺と腸管をつなぐ粘膜免疫ネットワークの実態解明

    阿部未来, 藤田勇気, 盛田彰太郎, 高梨暁, 古川睦実, 新實香奈枝, 宇佐美克紀, 渡邊康一, 麻生久, 野地智法

    第20回日本乳房炎研究会　2015/10/09

  144. 乳汁中に黄色ブドウ球菌特異的IgA抗体を誘導させる鼻腔を介した抗原取り込み機序の解析

    長澤裕哉, 菊佳男, 野地智法, 麻生久, 田邉扶由子, 菅原和恵, 石川義春, 門田耕一, 林智人

    第158回日本獣医学会　2015/09/07

  145. X連鎖重症複合免疫不全症（X-SCID）疾患モデルブタを用いた骨髄移植治療による腸管でのリンパ組織新生に関する研究

    盛田彰太郎, 鈴木俊一, 大西彰, 渡邊康一, 麻生久, 野地智法

    第158回日本獣医学会　2015/09/07

  146. X連鎖重症複合免疫不全症（X-SCID)疾患モデルブタを用いた骨髄移植治療後の腸管関連リンパ組織新生に関する形態学的研究

    盛田彰太郎, 鈴木俊一, 渡邊康一, 麻生久, 大西彰, 野地智法

    第65回東北畜産学会　2015/08/28

  147. 枯草菌給与によるウシ乳房炎発症予防と血中免疫担当細胞の動態解析

    浦川めぐみ, 佐藤秀俊, 芦田延久, 今林寛和, 高梨暁, 渡邊康一, 野地智法, 麻生久

    第65回東北畜産学会　2015/08/28

  148. 授乳期の乳腺免疫の発達を促す腸管を中心とした粘膜免疫システムの実態とは

    新實香奈枝, 阿部未来, 藤田勇気, 盛田彰太郎, 古川睦実, 宇佐美克紀, 渡邊康一, 麻生久, 野地智法

    第2回乳房炎サマーキャンプ　2015/08/22

  149. トランスポゾン挿入変異法を用いたウシ乳房炎関連病原因子の解明に向けた基盤研究

    佐藤美佳, 勝部哲, 野地智法, 林智人, 秦英司, 菊佳男, 安藤太助, 麻生久, 磯貝恵美子, 米山裕

    第119回日本畜産学会　2015/03/28

  150. 乳房炎誘導牛における乳汁中シクロフィリンA濃度の定量分析と乳房炎早期発見への応用

    梅村紗緒里, 高梨暁, 板谷奈波, 浦川めぐみ, 菊佳男, 林智人, 渡邊康一, 野地智法, 麻生久

    第119回日本畜産学会　2015/03/28

  151. 反芻動物特異的I型筋線維サブタイプの免疫組織化学的特性の解明

    佐藤貴俊, 白須直樹, 櫻田隆大, 野地智法, 麻生久, 渡邊康一

    第119回日本畜産学会　2015/03/28

  152. 枯草菌給与による牛乳房炎発症予防効果

    佐藤秀俊, 浦川めぐみ, 佐藤勝祥, 板谷奈波, 芦田延久, 今林寛和, 高梨暁, 梅村紗緒里, 渡邊康一, 野地智法, 麻生久

    第119回日本畜産学会　2015/03/28

  153. 枯草菌給与による乳房炎牛の血中免疫担当細胞の動態解析

    浦川めぐみ, 佐藤秀俊, 佐藤勝祥, 板谷奈波, 芦田延久, 今林寛和, 高梨暁, 梅村紗緒里, 渡邊康一, 野地智法, 麻生久

    第119回日本畜産学会　2015/03/28

  154. 品種交雑が選抜ランドレース種豚の抗病性関連遺伝子に及ぼす影響

    高橋光, 菅原静夏, 長澤裕哉, 寺田俊介, 板谷奈波, 佐藤貴俊, 藤田勇気, 北澤春樹, 島津朋之, 鈴木啓一, 野地智法, 渡邊康一, 麻生久

    第119回日本畜産学会　2015/03/28

  155. セロトニン経口投与がマウス十二指腸セロトニン分泌細胞に与える影響

    日高湧介, 寺田俊介, 佐藤勝祥, 陳香凝, 浦川めぐみ, 渡邊康一, 野地智法, 麻生久

    第119回日本畜産学会　2015/03/28

  156. 乳腺免疫の要となるIgA産生を促す分子メカニズムの解明

    藤田勇気, 阿部未来, 盛田彰太郎, 立田睦実, 渡邊康一, 野地智法, 麻生久

    第119回日本畜産学会　2015/03/28

  157. 乳房炎発症と乳汁中シクロフィリン分泌量の関連性

    梅村紗緒里, 高梨暁, 板谷奈波, 佐藤勝祥, 浦川めぐみ, 菊佳男, 林智人, 渡邊康一, 野地智法, 麻生久

    第19回日本乳房炎研究会　2014/10/10

  158. 乳腺組織に効果的な免疫応答を誘導するためのワクチネーションとは

    藤田勇気, 阿部未来, 秦英司, 菊佳男, 林智人, 渡邊康一, 野地智法, 麻生久

    第19回日本乳房炎研究会　2014/10/10

  159. 枯草菌給与による乳房炎発症予防効果の検討

    佐藤勝祥, 佐藤秀俊, 浦川めぐみ, 板谷奈波, 高梨暁, 梅村紗緒里, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第19回日本乳房炎研究会　2014/10/10

  160. 枯草菌給与が乳牛の血中免疫担当細胞へ与える影響

    浦川めぐみ, 佐藤勝祥, 佐藤秀俊, 板谷奈波, 高梨暁, 梅村紗緒里, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第19回日本乳房炎研究会　2014/10/10

  161. ウシ骨格筋への低周波電気刺激によるID型筋線維の誘導

    佐藤貴俊, 白須直樹, 櫻田隆大, 野地智法, 麻生久, 渡邊康一

    第64回東北畜産学会　2014/09/11

  162. 乳汁中シクロフィリン測定による乳房炎発症リスクの定量的分析

    梅村紗緒里, 高梨暁, 板谷奈波, 佐藤勝祥, 浦川めぐみ, 菊佳男, 林智人, 渡邊康一, 野地智法, 麻生久

    第64回東北畜産学会　2014/09/11

  163. 枯草菌給与による乳牛の血中免疫担当因子への影響

    浦川めぐみ, 佐藤勝祥, 佐藤秀俊, 板谷奈波, 高梨暁, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第64回東北畜産学会　2014/09/11

  164. 枯草菌給与による乳房炎発症予防効果の検討と分娩前後の栄養状態への影響

    佐藤勝祥, 佐藤秀俊, 浦川めぐみ, 板谷奈波, 高梨暁, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第64回東北畜産学会　2014/09/11

  165. 新生仔ブタ腸管に認められる初乳取り込み能を持つ上皮細胞の組織学的研究

    盛田彰太郎, 長澤裕哉, 渡邊康一, 野地智法, 麻生久

    第157回日本獣医学会　2014/09/09

  166. IgA産生細胞を効果的に乳腺組織に遊走させるための免疫戦略の確立

    藤田勇気, 阿部未来, 秦英司, 菊佳男, 林智人, 渡邊康一, 野地智法, 麻生久

    第157回日本獣医学会　2014/09/09

  167. 乳房炎牛乳汁中に増量するシクロフィリンＡの細胞遊走活性

    高梨暁, 梅村紗緒里, 板谷奈波, 阿部未来, 佐藤勝祥, 浦川めぐみ, 渡邊康一, 野地智法, 麻生久

    第1回乳房炎サマーキャンプ　2014/08/09

  168. 乳腺免疫のふしぎ

    野地智法

    第1回乳房炎サマーキャンプ　2014/08/09

  169. マウスへのセロトニン経口投与における糖代謝および脂質代謝への影響

    佐藤勝祥, 寺田俊介, 住吉敬介, 陳香凝, 北澤春樹, 白川仁, 渡邊康一, 野地智法, 麻生久

    第118回日本畜産学会　2014/03/27

  170. セロトニン腹腔内投与が高脂肪飼料摂取マウスの腸管セロトニン分泌細胞に与える影響

    住吉啓介, 渡邉一史, 齋藤遼, 中野辰也, 長澤裕哉, 陳香凝, 岡田夏実, 渡邊康一, 野地智法, 麻生久

    第118回日本畜産学会　2014/03/27

  171. 乳房炎牛乳汁中に増量するシクロフィリンAの細胞遊走活性

    高梨暁, 板谷奈波, 阿部未来, 佐藤勝祥, 浦川めぐみ, 渡邊康一, 野地智法, 麻生久

    第118回日本畜産学会　2014/03/27

  172. ウシ最長筋における筋線維型移行に伴う筋タンパク質分解系の発現解析

    白須直樹, 佐藤貴俊, 櫻田隆大, 野地智法, 麻生久, 渡邊康一

    第118回日本畜産学会　2014/03/27

  173. 抗病性選抜ランドレース豚の免疫特性解析

    菅原静夏, 高橋光, 長澤裕哉, 寺田俊介, 白須直樹, 住吉敬介, 板谷奈波, 北澤春樹, 島津朋之, 鈴木啓一, 野地智法, 渡邊康一, 麻生久

    第118回日本畜産学会　2014/03/27

  174. マウス褐色脂肪細胞株の樹立

    陳香凝, 長澤裕哉, 渡邉一史, 齋藤遼, 住吉敬介, 岡田夏美, 佐藤勝祥, 渡邊康一, 野地智法, 麻生久

    第118回日本畜産学会　2014/03/27

  175. In vivo modeling of HIV pathogenesis in the human gut-associated lymphoid tissue (GALT)

    Tomonori Nochi, Paul W. Denton, Angela Wahl, J. Victor Garcia

    第42回日本免疫学会　2013/12/11

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    本人の発表

  176. Recent progress in mucosal vaccine development International-presentation

    Tomonori Nochi

    The 2nd International Mini-symposium on "New Challenges for Animal Science Research"　2013/11/26

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    本人の発表

  177. 枯草菌給与による乳牛の乳房炎予防

    佐藤英俊, 浦川めぐみ, 佐藤勝祥, 板谷奈波, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第18回日本乳房炎研究会　2013/10/11

  178. 枯草菌給与によるウシ乳房炎発症予防と血中栄養因子およびストレス指標の変動

    佐藤勝祥, 浦川めぐみ, 板谷奈波, 佐藤英俊, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第18回日本乳房炎研究会　2013/10/11

  179. 枯草菌給与によるウシ乳房炎発症予防と血中樹状細胞の動態

    浦川めぐみ, 佐藤勝祥, 板谷奈波, 佐藤英俊, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第18回日本乳房炎研究会　2013/10/11

  180. ヒト化マウスのヒト腸管関連リンパ組織の解析を目的とした組織学的研究

    野地智法, Paul W. Denton, Angela Wahl, J. Victor Garcia

    第156回日本獣医学会　2013/09/20

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    本人の発表

  181. マウス腸管M細胞における解糖系酵素アルドラーゼAを介した異常型プリオン蛋白質取り込み機構

    長澤裕哉, 盛田彰太郎, 日高湧介, 渡邉一史, 大和田修一, 北澤春樹, 今村守一, 横山隆, 堀内基広, 坂口末廣, 西田教行, 渡邊康一, 野地智法, 麻生久

    第156回日本獣医学会　2013/09/20

  182. 乳牛の乳房炎発症に与える枯草菌給与の影響

    佐藤英俊, 浦川めぐみ, 佐藤勝祥, 板谷奈波, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第117回日本畜産学会　2013/09/09

  183. 乳房炎発症牛への枯草菌給与における栄養因子への影響

    佐藤勝祥, 浦川めぐみ, 板谷奈波, 佐藤英俊, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第117回日本畜産学会　2013/09/09

  184. 乳房炎発症牛への枯草菌給与における血中樹状細胞の動態

    浦川めぐみ, 佐藤勝祥, 板谷奈波, 佐藤英俊, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第117回日本畜産学会　2013/09/09

  185. ヒト腸管関連リンパ組織でのHIV感染機序の解明を目的としたヒト化マウス研究

    野地智法, Paul W. Denton, Angela Wahl, J. Victor Garcia

    第67回日本細菌学会東北支部総会　2013/08/30

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    本人の発表

  186. プロバイオティクスを利用した乳房炎の発症予防

    佐藤英俊, 浦川めぐみ, 佐藤勝祥, 板谷奈波, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第63回東北畜産学会　2013/08/29

  187. 乳房炎発症牛への枯草菌給与における分娩後の栄養因子およびストレス指標の変動

    佐藤勝祥, 浦川めぐみ, 板谷奈波, 佐藤英俊, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第63回東北畜産学会　2013/08/29

  188. 乳房炎多発症牛への枯草菌給与による乳房炎発症予防と血中樹状細胞の動態

    浦川めぐみ, 佐藤勝祥, 板谷奈波, 佐藤英俊, 芦田延久, 今林寛和, 渡邊康一, 野地智法, 麻生久

    第63回東北畜産学会　2013/08/29

  189. In vivo modeling of human GALT development and pathogenesis using xenotransplantation International-presentation

    Tomonori Nochi, Paul W. Denton, Angela Wahl, J. Victor Garcia

    16th International Congress of Mucosal Immunology　2013/07/17

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    本人の発表

  190. In vivo modeling of human GALT development and pathogenesis using xenotransplantation

    Tomonori Nochi, Paul W. Denton, Angela Wahl, J. Victor Garcia

    NIH-Tohoku University JSPS Symposium　2013/05/09

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    本人の発表

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Industrial Property Rights 3

  1. 乳汁中のIgA抗体含有量の増加剤

    野地智法, 宇佐美克紀, 麻生久, 清野宏, 藤橋浩太郎, 佐藤慎太郎

    特許7411910

    Property Type: Patent

  2. カチオン性ナノゲルを用いる粘膜ワクチン

    秋吉一成, 清野 宏, 幸 義和, 野地智法

    特許5344558

    Property Type: Patent

  3. ワクチン遺伝子導入イネ

    幸義和, 清野宏, 廣井隆親, 野地智法, 高岩文雄, 高木英典, 楊麗軍, 鈴木一矢

    特許4769977

    Property Type: Patent

Research Projects 20

  1. 子牛への有用微生物叢移植ガイドライン作成事業

    野地智法, Jahidul Islam, 古川睦実, 平川良太, 田中秀和, 大谷夏輝, 山﨑隼也

    Offer Organization: 全国競馬・畜産振興会（JRA）

    System: 畜産振興事業

    2025/06 - 2029/03

  2. Development of an Optimal Method for Generating Humanized Pigs for Practical Applications and the Reconstitution of Anti-Tumor Immune Responses

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Nihon University

    2025/04/01 - 2028/03/31

  3. 乳汁抗体が有する“有用”微生物に対する特異的な作用機序

    野地智法, 濵口祐, 北澤春樹, Jahidul Islam, 西山啓太, 古川睦実, 平川良太

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2025/06 - 2027/03

  4. 微生物叢改善による子牛の健全育成事業

    野地智法

    System: みちのくGAPファンド（Step1）

    2025/06 - 2026/03

  5. Clustering of plasma cells for a comprehensive understanding of immune functions in the mammary glands

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2022/04 - 2026/03

  6. 乳幼児の腸内フローラ形成を助長するミルク開発

    Offer Organization: 三菱財団

    System: 自然科学研究助成

    Institution: 東北大学

    2024/10 - 2025/09

  7. Global research for sustainable livestock production using useful microbiota

    Offer Organization: Tohoku Universityu

    2023/06 - 2025/03

  8. 鳥類の免疫学におけるパラダイムシフトによる鶏のB細胞分化に関する概念の再構築

    野地智法, 古川睦実

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2023/06 - 2025/03

  9. 幼若豚の健全育成を可能にする母豚および仔豚用プロバイオティクス開発

    野地智法, 古川睦実

    Offer Organization: JST

    System: A-STEP（育成型）

    Institution: 東北大学

    2022/10 - 2025/03

  10. 糞便移植の本格普及による子牛の下痢症制御

    野地智法, Jahidul Islam, 古川睦実, 米山裕, 酒井義文, 田中秀和, 中村善, 山崎隼也

    Offer Organization: 全国競馬・畜産振興会（JRA）

    System: 畜産振興事業

    Institution: 東北大学

    2022/04 - 2025/03

  11. 腸管を基軸とした幼若雛の健全育成を支えるニワトリ粘膜免疫学の創成

    野地 智法、Jahidul Islam、佐藤幹、喜久里基、陶山佳久、松尾歩

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的研究(萌芽)

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2021/07 - 2023/03

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    ニワトリ（鶏）の免疫学に関する教科書には、骨髄で造血幹細胞から分化したB細胞前駆細胞は、排泄腔近傍に存在するファブリキウス嚢でB細胞へと分化し、その後、脾臓やパイエル板（回腸終末部）、盲腸扁桃（盲腸基部）といった免疫臓器で機能すると記載されている（Avian Immunology 2nd Edition）。一方で、我々は、広大な表面積を有する盲腸にも成熟したB細胞が多数集積する濾胞構造が広範囲に発達していることを見出し、盲腸扁桃やパイエル板とは異なる、腸管に発達する新たな免疫臓器（Cecal patches: CP）の存在を明らかにしてきた。加えて、経時的に実施した形態学的解析を通して、CPは盲腸扁桃と同様に孵化後に形態形成されること、腸管の免疫機能に深く関わるIgA（免疫グロブリンのサブクラスの１つ）を産生する形質細胞へのB細胞からの最終分化が、盲腸扁桃やパイエル板以上に、CPで盛んに行われていることを見出し、既存の教科書には記載されていない、孵化後の鶏の腸管内で発達するCPを中心とした粘膜免疫システムの存在を提唱してきた。 <BR> そこで本研究では、孵化1, 2, 3, 5, 7 週間後の肉用鶏（Ross308系）より、盲腸を採材・固定し、CPの形態形成を組織学的に解析した。また、組織切片をIgM, IgA, IgYをマーカーとした免疫組織化学染色に供することで、抗体産生細胞の局在変化も比較解析した。その結果、CP形成が開始される時期は、盲腸扁桃の発達時期よりも少し遅く、孵化2週齢以降であることが明らかになった。このことは、盲腸扁桃とCPの形態形成を促す刺激は異なっている可能性を強く示唆していた。さらには、CPで濾胞構造が発達する時期は3週齢前後であり、同時期に濾胞内でB細胞からIgA産生細胞への最終分化が起きていることも明らかになった。

  12. 母子移行型の乳汁免疫強化を可能にする母豚用プロバイオティクス開発

    野地智法, 古川睦実, Islam Jahidul

    Offer Organization: JST

    System: 研究成果最適展開支援プログラム（A-STEP）産学共同（育成型）

    Institution: 東北大学

    2021/04 - 2022/03

  13. 子牛への糞便移植の技術基盤形成と疾病制御事業

    野地智法、Islam Jahidul、米山裕、陶山佳久、酒井義文、麻生久、田中秀和、清水優

    Offer Organization: 全国競馬・畜産振興会（JRA）

    System: 畜産振興事業

    2019/04 - 2022/03

  14. Elucidation of reproductive cycle-specific immunological and microbiological dynamics using bioimaging

    Nochi Tomonori

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2018/04 - 2022/03

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    The evolutionary strategy of transferring maternal antibodies via milk profoundly impacts the survival, lifelong health and wellbeing of all neonates. While there has been increased recognition that interorgan connectivity influences the quality of a mother’s milk, potentially to personalize it for her offspring, the underlying bases for these processes are incompletely resolved. Here we define an essential role of Peyer’s patches (PP) for the generation of plasma cells that secrete maternal IgA into milk. Our metagenomic analysis reveals that the presence of certain residential microorganisms in the gastrointestinal tract, such as Bacteroides acidifaciens and Prevotella buccalis, is indispensable for the programming of maternal IgA synthesis prior to lactational transfer. Our data provide important insights into how the microbiome of the maternal gastrointestinal environment, specifically through the PP, can be communicated to the next generation via milk.

  15. Elucidation of the organ-network between the mammary glands and intestine to enhance maternal immunity

    NOCHI TOMONORI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Challenging Research (Exploratory)

    Category: Challenging Research (Exploratory)

    Institution: Tohoku University

    2017/06 - 2020/03

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    In this study, we demonstrated the development of microbial environment in milk of humans, cows and mice, and such bacteria differ among the host species. Since the maternal immunity is affected by microorganisms that cohabit in the host, we tried to improve the microflora by inoculation of lactic acid bacteria (CRL1505) that has been used as a probiotics to stimulate mucosal immunity. However, the level of maternal antibody production was not changed by administration with CRL1505. Given that the disturbance of microflora affect the level of maternal IgA production in milk, these results suggested that some microorganisms (not lactic acid bacteria) present in mucosal tissues may be involved in inducing maternal antibody transfer to the offspring.

  16. 医療用免疫不全モデルブタを用いた原発性免疫不全症候群に対する新規治療技術開発に向けた基盤研究

    野地智法、鈴木俊一、板野理、北郷実、笹原洋二

    Offer Organization: 日本医療研究開発機構（AMED）

    System: 難治性疾患実用化研究事業

    2016/04 - 2019/03

  17. Elucidation of molecular mechanism that promotes IgA production in the mammary gland

    Nochi Tomonori, Aso Hisashi, Watanabe Kouichi, Kiyono Hiroshi, Sato Shintaro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)

    Category: Grant-in-Aid for Young Scientists (A)

    Institution: Tohoku University

    2016/04 - 2019/03

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    Breastfeeding is important for mammals, providing immunological and microbiological advantages to neonates from the mother. However, the mechanisms of this functional diversity in the mammary gland remain poorly characterized. Here, we show that the mammary gland develops immune and microbial environments consisting of IgA and the microflora, respectively, both of which are important for protecting neonates and the mother from infectious diseases. The IgA production and microflora development are coordinated in the gastrointestinal tract but seem to be independently regulated in the mammary gland. In particular, CCL28, a crucial molecule for the recruitment of IgA-producing cells into the mammary gland, was expressed normally in germ-free lactating mice but were almost undetectable in postweaning mothers, regardless of the microflora presence. Our findings offer insights into potentially improving the quality of breastfeeding, using both immunological and microbiological approaches.

  18. Development of novel technology for prevention of mastitis using nanotechnology

    Nochi Tomonori

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2014/04 - 2017/03

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    Staphylococcus aureus is a major pathogen that occasionally causes subclinical mastitis associated with huge economic losses to the dairy industry. We generated S. aureus-specific bovine IgG antibodies (anti-S. aureus) that directly inhibited bacterial growth in vitro. Inhibition depended on specificity for anti-S. aureus. An in vitro culture study using S. aureus strain JE2 and its deletion mutant JE2ΔSrtA, which lacks the gene encoding sortase A, revealed that the effect of anti-S. aureus was sortase-A-independent. Sortase A is involved in the synthesis of cell-wall-associated proteins. Thus, other surface molecules, such as membrane proteins, cell surface polysaccharides, or both, may trigger the inhibition of bacterial growth by anti-S. aureus. Together, our findings contribute insights into developing new strategies to further improve the available mastitis vaccine by designing a novel antigen on the surface of S. aureus to induce inhibitory signals that inhibit bacterial growth.

  19. ヒトの腸管免疫系を発達させたヒト化マウスの作製

    野地 智法

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 研究活動スタート支援

    Category: 研究活動スタート支援

    Institution: 東北大学

    2013/08 - 2015/03

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    研究初年度にあたる平成２５年度は、申請課題である「ヒトの腸管免疫系を発達させたヒト化マウスの作製」を達成すべく、平成２５年度に発表した論文（Cell Reports, 3: 1-11, 2013)の中で報告した、ヒト化BLTマウスの小腸組織におけるヒトの腸管関連リンパ組織の発達とHIV感染に関する結果に加え、大腸組織におけるヒトの腸管関連リンパ組織の発達とHIV感染に関する解析を行った。 ヒト化BLTマウスの大腸組織を切片免疫組織学的解析を行った結果、NOD/SCIDマウスをレシピエントマウスとして作製したヒト化BLTマウスには、非常によく発達した腸管関連リンパ組織が認められた。また、ヒトのT細胞、B細胞、樹状細胞、マクロファージといった、全てのヒトの免疫担当細胞がそこに集結していることが明らかとなった。しかしながら、IL-2Rｇ鎖を欠損させたNOD/SCIDマウス（通称NSGマウス）をレシピエントマウスとして用いて作製したヒト化BLTマウスの大腸には、ヒトの腸管関連リンパ組織の発達は全く認められなかった。 次にHIVを感染させたヒト化BLTマウス（NOD/SCIDマウスを用いて作製）の大腸組織を、in situ hybridization法により解析したところ、非常に多くのHIV感染細胞が大腸の腸管関連リンパ組織に存在していることが明らかとなった。 以上の結果から、IL-2Rｇ鎖を発現する免疫不全マウス（例：NOD/SCIDマウス）を用いてヒト化BLTマウスを作製することは、ヒトの腸管免疫系を小腸のみならず大腸にも発達させるために非常に重要であることが明らかとなった。さらには、大腸の粘膜関連リンパ組織が、HIVの感染標的組織に成り得ることも明らかとなり、今年度の目標を十分達成することが出来た。

  20. ボツリヌス感染症の予防法の確立を目的とした"M細胞標的型食べるワクチン"開発

    野地 智法

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 東京大学

    2008 - 2010

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    研究最終年度に当たる今年度は、申請課題であるコメ型ワクチンを完成させるべく、トランスレーショナルリサーチを組み合わせた研究を実施した。具体的には、コメ型ワクチンのヒトでの免疫原性を評価すべく、日本学術振興会特別研究員の規定の下、ヒト化マウス研究の権威である、米国ノースカロライナ大学のJ.Victor Garcia-Martinez博士の研究室に研究留学し、ヒト化マウスの腸管組織におけるヒトの粘膜免疫学の発達に関する評価を行った。その結果、これまで多数報告されているヒト化マウスの中で、Garcia研究室で作成するヒト化マウス(BLTマウス)は、腸管免疫システムが最も発達しているモデル動物であること、そして粘膜免疫システムで最も重要であるIgA産生が、このBLTマウスのみに高頻度で認められることを明らかにし、粘膜ワクチン研究に最も適したモデル動物であることを明らかにすることに成功した(論文準備中)。一方で、2007年度に申請者が開発し報告したコメ型コレラワクチンが、コレラ毒素に対する防御免疫誘導能に加えて、コレラ菌や病原性大腸菌(生菌)に対する防御免疫も誘導可能であることも、実証することに成功した(PNAS,107:8794-8789,2010)。その他、今年度は、安全性に優れた経鼻ワクチン開発にも力を注ぎ、ナノメートルサイズの粒子(ナノゲル)をワクチンデリバリーとして応用したナノゲル型経鼻ワクチンの開発にも成功し、論文として発表した(Nature Materials、9:572-578,2010)。以上の通り、今年度は、計5報の原著論文を発表し、また本研究期間である平成20-22年の3年間を通して、計18報の論文を発表することができた。3年間の本研究により、粘膜ワクチンを実用化するための、研究基盤を構築することができたと考える。

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Teaching Experience 7

  1. Food histology　Tohoku University

  2. Animal tissue cytology　Tohoku University

  3. International Food & Agricultural Immunology Lecture　Tohoku University Graduate School of Agricultural Science

  4. Food & Agricultural Immunology Joint Lecture　Tohoku Universtiy Graduate School of Agricultural Science

  5. Current agriculture　Tohoku University

  6. Animal Functional Morphology　Tohoku University

  7. Advanced studies in functions of tissues and cells　Tohoku University

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Social Activities 17

  1. インドネシアIPB Visiting Professor

    2024/10/28 - 2024/11/04

  2. 腸管における免疫と微生物のクロストーク

    セミナー/勉強会「免疫から考える微生物共生」

    2024/12/09 -

  3. 静岡県立浜松西高等学校 大学出前講義

    2024/09/05 -

  4. 静岡県立浜松西高等学校 大学出前講義

    2023/09/05 -

  5. 静岡県立浜松西高等学校 大学出前講義

    2022/09/06 -

  6. 静岡県立浜松西高等学校 大学出前講義

    2021/09/02 -

  7. 静岡県立浜松西高等学校 大学出前講義

    2020/09/03 -

  8. 東京オトナ大学

    2019/12/01 -

  9. 夢ナビライブ2019（仙台）

    2019/10/05 -

  10. 静岡県立浜松西高等学校 大学出前講義

    2019/09/05 -

  11. 夢ナビライブ2019（東京）

    2019/06/08 -

  12. 静岡県立浜松西高等学校 大学出前講義

    2018/12/08 -

  13. 免疫は体を守る？それとも病気にさせる？免疫いいこと、悪いこと。

    サイエンスカフェ

    2018/01/16 -

  14. 静岡県立浜松西高等学校 大学出前講義

    2017/12/09 -

  15. 浜松西高等学校 大学出前講義

    2016/12/10 -

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    静岡県立浜松西高等学校での出張講義

  16. 大学出前講義

    2015/05/20 -

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    宮城県泉高等学校での出張講義

  17. 大学出前講義

    2014/10/25 -

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    静岡県立沼津東高等学校での出張講義

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Media Coverage 9

  1. 乳 進化する神秘の液体

    NHK　ヒューマニエンス

    2023/07

    Type: TV or radio program

  2. 動物薬にも広がる腸内細菌創薬

    日経バイオテク

    2023/06

    Type: Internet

  3. ふん移植で子牛“快腸”

    日本農業新聞

    2023/06

    Type: Newspaper, magazine

  4. 腸内微生物叢改善による子牛の難治性下痢症の改善 ～糞便移植の有効性を裏付ける科学的メカニズムを探索～

    東北大学　https://www.tohoku.ac.jp/japanese/2022/02/press20220221-02-treatment.html

    2022/02

  5. 腸管と乳腺はつながっている！ ～腸内微生物が母乳中の抗体産生を促す～

    東北大学 https://www.tohoku.ac.jp/japanese/2021/09/press20210908-01.html

    2021/09

    Type: Internet

  6. 2019年ニュース 「東京オトナ大学」にて農学研究科 野地智法准教授が講義を行いました

    東北大学　https://www.tohoku.ac.jp/japanese/2019/12/news20191210-01.html

    2019/12

    Type: Internet

  7. 動物が秘めた「免疫」の可能性を探る

    夢ナビ　https://yumenavi.info/lecture.aspx?GNKCD=g009552&OraSeq=9593099&ProId=WNA002&SerKbn=c&SearchMod=3&Page=4&KeyWord=%E6%9D%B1%E5%8C%97%E5%A4%A7%E5%AD%A6

    2019/10

    Type: Internet

  8. 【TOHOKU University Researcher in Focus】Vol.003 ミルクが教える免疫の不思議ー母乳の潜在力に迫るー

    東北大学　https://www.tohoku.ac.jp/japanese/2019/08/infocus-003.html

    2019/08

    Type: Internet

  9. 東北大と東大、授乳期の乳腺に免疫・微生物環境が発達する仕組みを解明

    日本経済新聞　https://www.nikkei.com/article/DGXLRSP466840_R21C17A2000000/

    2017/12

    Type: Newspaper, magazine

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Academic Activities 4

  1. 第８回乳房炎サマーキャンプ

    2024/09/01 - 2024/09/02

    Activity type: Competition, symposium, etc.

  2. Tohoku Forum for Creativity: New Horizons in Food Science via Agricultural Immunology

    2017/07/21 - 2017/09/23

    Activity type: Competition, symposium, etc.

  3. 第５回乳房炎サマーキャンプ

    2018/09/04 -

  4. 第２回乳房炎サマーキャンプ

    2015/08/02 -

    Activity type: Academic society, research group, etc.

Other 6

  1. 東北大学高等大学院機構 食科学国際共同大学院プログラム 代表者

  2. Future Principal Investigator Program

  3. EMBO Lab Leadership for Group Leaders 受講

  4. 東北大学高度教養教育・学生支援機構大学教育支援センター 大学教員準備プログラム（PFFP）、新任教員プログラム（NFP）先達教員

  5. 東北大学高度教養教育・学生支援機構大学教育支援センター アカデミック・リーダー育成プログラム（LAD）受講

  6. 東北大学高度教養教育・学生支援機構大学教育支援センター 新任教員プログラム（NFP）受講

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