Details of the Researcher

PHOTO

Genki Hibi
Section
Graduate School of Pharmaceutical Sciences
Job title
Specially Appointed Research Fellow
Degree

  • Doctor of Agriculture (The University of Tokyo)

e-Rad No.
31017338

Research History 2

  • 2024/08 - Present
    Tohoku University　Graduate School of Pharmaceutical Sciences

  • 2024/04 - 2024/07
    The University of Tokyo　Graduate School of Agricultural and Life Sciences

Education 4

  • The University of Tokyo　Graduate School of Agricultural and Life Sciences　Biotechnology

    2021/04 - 2024/03

  • The University of Tokyo　Graduate School of Agricultural and Life Sciences　Biotechnology

    2019/04 - 2021/03

  • The University of Tokyo

    2017/04 - 2019/03

  • The University of Tokyo　College of Arts and Sciences

    2015/04 - 2017/03

Research Interests 1

  • 生合成

Awards 4

  1. 2024年度 最優秀ポスター賞

    2024/09　2024年度 日本放線菌学会大会　放線菌が生産する炭素五員環骨格含有天然化合物の探索

  2. 令和5年度農学生命科学研究科 研究科長賞

    2024/03　東京大学大学院 農学生命科学研究科

  3. 優秀発表賞

    2022/08　日本農芸化学会関東支部　特殊アミノ酸シスペンタシンの生産とその生合成機構の解析

  4. トピックス賞

    2022/04　日本農芸化学会　アミプリマイシンに含まれる特殊アミノ酸の生合成機構の解析

Papers 2

  1. Identification of the biosynthetic gene cluster for PF1451A: Implications for untapped peptide biosynthetic pathways widely distributed in fungi

    Genki Hibi, Taro Ozaki, Riku Sugimoto, Yohei Morishita, Teigo Asai

    Bioscience, Biotechnology, and Biochemistry　2026/05/21

    DOI： 10.1093/bbb/zbag074 　

  2. Discovery of type II polyketide synthase-like enzymes for the biosynthesis of cispentacin. International-journal

    Genki Hibi, Taro Shiraishi, Tatsuki Umemura, Kenji Nemoto, Yusuke Ogura, Makoto Nishiyama, Tomohisa Kuzuyama

    Nature communications　14　(1)　8065-8065　2023/12/06

    DOI： 10.1038/s41467-023-43731-z 　

    More details Close

    Type II polyketide synthases (PKSs) normally synthesize polycyclic aromatic compounds in nature, and the potential to elaborate further diverse skeletons was recently revealed by the discovery of a polyene subgroup. Here, we show a type II PKS machinery for the biosynthesis of a five-membered nonaromatic skeleton contained in the nonproteinogenic amino acid cispentacin and the plant toxin coronatine. We successfully produce cispentacin in a heterologous host and reconstruct its biosynthesis using seven recombinant proteins in vitro. Biochemical analyses of each protein reveal the unique enzymatic reactions, indicating that a heterodimer of type II PKS-like enzymes (AmcF-AmcG) catalyzes a single C2 elongation as well as a subsequent cyclization on the acyl carrier protein (AmcB) to form a key intermediate with a five-membered ring. The subsequent reactions, which are catalyzed by a collection of type II PKS-like enzymes, are also peculiar. This work further expands the definition of type II PKS and illuminates an unexplored genetic resource for natural products.

Misc. 1

  1. 炭素五員環骨格を形成する新規Ⅱ型ポリケチド合成酵素の発見

    日比玄紀, 葛山智久

    バイオサイエンスとインダストリー(B&I）　82　(5)　492-493　2024/09

Research Projects 1

  1. 真菌特有ペプチド骨格形成マシナリーの試験管内再構成

    日比 玄紀

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 研究活動スタート支援

    Institution: 東北大学

    2025/07/31 - 2027/03/31