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Daisuke Sano

Section

Graduate School of Engineering

Job title

Professor

Degree

博士（工学）（東北大学）
修士（工学）（東北大学）

researchmap

https://researchmap.jp/read0065526

J-GLOBAL ID

200901034718778778

Research History 9

2021/04 - Present

Tohoku University　Department of Civil and Environmental Engineering, Graduate School of Engineering　Professor
2015/04 - Present

The University of Kitakyushu
2018/04 - 2021/03

Tohoku University　Department of Frontier Sciences for Advanced Environment, Graduate School of Environmental Studies　Associate Professor
2017/04 - 2018/03

Tohoku University　Department of Civil and Environmental Engineering　Associate Professor
2009/04 - 2017/03

Hokkaido University　Faculty of Engineering　Associate Professor
2015/05 - 2015/06

University of Barcelona　Department of Microbiology, Faculty of Biology　Visiting Associate Professor
2007/04 - 2009/03

University of Barcelona　Department of Microbiology, Faculty of Biology　JSPS Fellow for Research Abroad
2003/04 - 2007/03

Tohoku University　Graduate School of Engineering　JSPS Research Fellow (PD)
2002/04 - 2003/03

Tohoku University　Faculty of Engineering　JSPS Research Fellow (DC2)

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Education 6

Tohoku University

- 2003
Tohoku University　Graduate School, Division of Engineering　Department of Civil Engineering

- 2003
Tohoku University

- 2000
Tohoku University　Graduate School, Division of Engineering　Department of Civil Engineeing

- 2000
Tohoku University　Faculty of Engineering

- 1998
Tohoku University　Faculty of Engineering　Department of Civil Engineering

- 1998

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Committee Memberships 25

Japan Society on Water Environment　Director

2021/07 - Present
Health-Related Water Microbiology study group (IWA)　Secretary

2017/05 - Present
平成２７年度総合地球環境学研究所　共同研究員

2015/10 - Present
日本水環境学会・水中健康関連微生物研究委員会　幹事

2014/04 - Present
Journal of Water and Health　Editor

2013/10 - Present
日本水環境学会　運営幹事

2013/08 - 2021/06
Water and Environment Technology Conference　Secretary

2013/11 - 2017/08
Health-Related Water Microbiology study group (IWA)　Steering committee member

2013/09 - 2017/05
土木学会・環境工学委員会　次世代下水道小委員会委員

2015/06 - 2017/03
土木学会・環境工学委員会　副幹事長

2015/04 - 2017/03
北海道大学大学院工学研究院　国際交流室

2014/04 - 2017/03
北海道大学大学院工学研究院　ヒトを対象とする研究倫理審査委員会

2014/04 - 2017/03
日本水環境学会北海道支部　幹事

2013/04 - 2017/03
土木学会　環境工学委員会

2011/04 - 2017/03
46. International Symposium for Food and Environmental Virology 2016　Secretary of Organizing Committee

2015/02 - 2016/10
Sapporo Pioneer Toastmaster Club　Vice President Membership

2015/07 - 2016/06
経済産業省北海道経済産業局・ミルキングパーラー排水の低コスト排水処理施設モデル構築のための検討ワーキング会議　委員長

2015/09 - 2016/03
札幌市　環境影響評価審議会委員

2014/09 - 2015/08
Sapporo Pioneer Toastmaster Club　President

2014/07 - 2015/06
北海道大学工学部・日韓共同理工系学部留学生等専門部会　座長

2014/05 - 2015/03
土木学会・環境工学委員会　今後の水環境保全に貢献する下水道システムの技術的課題と管理手法についての調査研究に関する小委員会

2012/06 - 2015/03
経済産業省北海道経済産業局・北海道根釧地域における環境対策支援のための検討ワーキング会議　委員長

2014/08 - 2015/02
Sapporo Pioneer Toastmaster Club　Vise President Education

2013/07 - 2014/06
土木学会論文集編集委員会　G分冊編集小委員会

2012/06 - 2014/05
土木学会・環境工学委員会　下水道関連震災調査小委員会

2012/02 - 2013/03

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Professional Memberships 8

ウイルス性下痢症研究会
THE JAPANESE SOCIETY FOR VIROLOGY
臨床ウイルス学会
American Chemical Society
International Water Association
American Society for Microbiology
日本水環境学会
土木学会

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Research Interests 7

ノロウイルス
腸内細菌
水環境中動態
消毒
ウイルス吸着タンパク質
感染症
Civil and Environmental Engineering

Research Areas 4

Life sciences / Hygiene and public health (non-laboratory) /
Life sciences / Hygiene and public health (laboratory) /
Life sciences / Healthcare management, medical sociology /
Social infrastructure (civil Engineering, architecture, disaster prevention) / Civil engineering (environmental systems) /

Awards 21

論文賞

2018/06　日本水環境学会　Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane
総長研究賞（奨励賞）

2016/02　北海道大学
若手奨励賞

2014/06　日本臨床ウイルス学会
第50回環境工学研究フォーラム優秀ポスター発表賞

2013/11　土木学会・環境工学委員会
第49回環境工学研究フォーラム優秀ポスター発表賞

2012/11　土木学会・環境工学委員会
メタウォーター賞（年間優秀論文賞）

2011/09　日本水環境学会
文部科学大臣表彰若手科学者賞

2008
The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology, The Young Scientists' Prize

2008
Presentation Award in the 7th meeting of Water Microbiology Specialist Group in Spanish Society of Microbiology

2008
土木学会環境工学研究フォーラム論文奨励賞

2007
土木学会論文奨励賞

2007
Encourage paper award from Japan Society for Civil Engineers

2007
Encourage paper award from Committee of Environmental Engineering, Japan Society for Civil Engineers

2007
土木学会東北支部技術開発賞

2006
Technological development award from northeast area chapter of Japan Society for Civil Engineers

2006
土木学会環境工学研究フォーラム論文賞

2005
Excellent paper award from Committee of Environmental Engineering, Japan Society for Civil Engineers

2005
土木学会優秀講演者表彰

2004/09
Best presentation award from Japan Society for Civil Engineers

2004
Grant for the research presentation in abroad by the Fundation for promotion of sewage system

2002
Sato Atsuhisa Fund for young researcher

2000

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Papers 160

State-space modelling using wastewater virus and epidemiological data to estimate reported COVID-19 cases and the potential infection numbers Peer-reviewed

Syun-suke Kadoya, Yubing Li, Yilei Wang, Hiroyuki Katayama, Daisuke Sano

Journal of The Royal Society Interface　22　20240456　2025/01/08

DOI： 10.1098/rsif.2024.0456 　
Quantitative microbial risk assessment of antibiotic-resistant Salmonella enterica contaminating hydroponic leafy vegetables Peer-reviewed

Shun Takayama, Qian Zhang, Ye Htut Zwe, Dan Li, Daisuke Sano, Wakana Oishi

Microbial Risk Analysis　27-28　100330-100330　2024/12
Publisher: Elsevier BV
DOI： 10.1016/j.mran.2024.100330 　

ISSN： 2352-3522
Quantitative evaluation of water, sanitation, and hygiene measures on COVID-19 pandemic peak reduction in the Philippines. International-journal

Sewwandi Bandara, Clyde Dapat, Wakana Oishi, Emmanuel Kagning Tsinda, Mayuko Saito, Daisuke Sano

The Science of the total environment　949　175055-175055　2024/11/01

DOI： 10.1016/j.scitotenv.2024.175055 　

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New cases of coronavirus disease 2019 (COVID-19) are continually being recorded worldwide, despite global efforts in implementing non-pharmaceutical interventions and establishing vaccination programs. This trend highlights the need to identify the factors associated with the continued spread of COVID-19. The World Health Organization recommends hand washing as a cost-effective intervention for preventing COVID-19, indicating that water, sanitation, and hygiene (WaSH) are central to the prevention of the disease. However, low- and middle-income countries lack adequate access to WaSH, which increases the risk of contracting COVID-19. The aim of this study was to identify the WaSH factors associated with the incidence of COVID-19 and quantitatively estimate the effects of improvements in WaSH on reducing the incidence of COVID-19 during the peak of the pandemic. Lasso regression and extreme gradient boosting models were used to identify the WaSH factors. Distinct estimation models were developed to assess the effect of WaSH in rural regions under two assumptions: increasing regional basic sanitation coverage up to 25 % and 50%. The reduction in the incidence of COVID-19 during the peak of the pandemic was calculated for each rural region. The results of the analyses indicated that basic sanitation is important for reducing the incidence of COVID-19 in rural regions compared to urban regions in the Philippines. In addition, the results suggested that increasing basic sanitation coverage could reduce the incidence of COVID-19 by 2-66 %, alleviating the burden on healthcare facilities. This study indicates that improved basic sanitation infrastructure are needed in rural Philippines. The results of this study emphasise the significance of WaSH as an indicator of COVID-19 incidence, highlighting the need for its enhancement to enable the achievement of sustainable disease prevention and pandemic preparedness goals.
Identification of protein biomarkers in wastewater linked to the incidence of COVID-19 Peer-reviewed

Luhur Akbar Devianto, Mohan Amarasiri, Luyao Wang, Takehito Iizuka, Daisuke Sano

Science of The Total Environment　951　175649-175649　2024/11
Publisher: Elsevier BV
DOI： 10.1016/j.scitotenv.2024.175649 　

ISSN： 0048-9697
Identification of environmental, socioeconomic, water, sanitation, and hygiene (WaSH) factors associated with COVID-19 incidence in the Philippines: A nationwide modelling study. International-journal Peer-reviewed

Sewwandi Bandara, Clyde Dapat, Wakana Oishi, Emmanuel Kagning Tsinda, Lea Necitas G Apostol, Naoko Hirayama, Mayuko Saito, Daisuke Sano

The Science of the total environment　946　174214-174214　2024/10/10

DOI： 10.1016/j.scitotenv.2024.174214 　

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Despite the implementation of non-pharmaceutical interventions, the threat of coronavirus disease 2019 (COVID-19) remains significant on a global scale. Identifying external factors contributing to its spread is crucial, especially given the World Health Organization's recommendation emphasizing access to water, sanitation, and hygiene as essential in curbing COVID-19. There is a notable discrepancy in access to sanitation facilities, particularly evident in low- and middle-income countries. However, there is a lack of quantitative assessments regarding these factors. This study examines various environmental, socioeconomic, water, sanitation, and hygiene factors and their associations with COVID-19 incidence. All regions in the Philippines were categorized into clusters based on socioeconomic factors. A conceptual structural equation model (SEM) was developed using domain knowledge. The best-fitting SEM for each cluster was determined, and associations between factors and COVID-19 incidence were estimated. The correlation analysis revealed that rainfall, minimum temperature, and relative humidity were positively correlated with weekly COVID-19 incidence in urban regions. Maximum temperature, mean temperature, wind speed, and wind direction were negatively correlated with weekly COVID-19 incidence in rural regions, with time lags of 0, 3, and 7 weeks. In urban regions (Cluster 1), factors such as urbanization rate (1.00), area (-0.93), and population (0.54) were found to be associated with weekly COVID-19 incidence. Conversely, in rural regions (Cluster 2), factors including area (0.17), basic sanitation (0.84), and wind direction (0.83) showed associations with weekly COVID-19 incidence. These factors were causally associated with a latent variable reflecting the hidden confounders associated with COVID-19 incidence. It is important to note that sanitation factors were associated only in rural regions. Improving access to sanitation facilities in rural regions of the Philippines is imperative to effectively mitigate disease transmission in future pandemics. Identification of the causal effect of unobserved confounders with COVID-19 incidence is recommended for future research.
Community-based mpox and sexually transmitted disease surveillance using discarded condoms in the global south International-journal International-coauthorship Peer-reviewed

Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, John Jefferson V Besa, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Puey Ounjai, W.K.C.P. Werawatte, Ali Hosseini Rad S.M., Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Phitsanuruk Kanthawee, Marily Al-Dois, Rapong Bani, Wanwara Thuptiang, Sifat Khan, Ratana Tacharoenmuang, Bernadina Cynthia, S.P.H Spencer Vitharana, Natharin Ngamwongsatit, Hitoshi Ishikawa, Andrew C Singer, Naveen Kumar Devanga Ragupathi, Takashi Furukawa, Kazunari Sei, Asuka Nanbo, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Tanittha Chatsuwan, Paul G Higgins, Daisuke Sano, Anthony Kicic, Jürgen Kurt Rockstroh, Richard Siow, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe

The Lancet Infectious Diseases　24　(10)　e610-e613　2024/10
Publisher: Elsevier BV
DOI： 10.1016/s1473-3099(24)00514-0 　

ISSN： 1473-3099
Degradation of extracellular antibiotic resistance genes using peracetic acid (PAA) and performic acid (PFA) International-journal Peer-reviewed

Mohan Amarasiri, Hiroki Kobayashi, Takashi Furukawa, Daisuke Sano, Kazunari Sei

Environmental Technology & Innovation　103862-103862　2024/10
Publisher: Elsevier BV
DOI： 10.1016/j.eti.2024.103862 　

ISSN： 2352-1864
Network-based Virus Dynamic Simulation: Evaluating the Fomite Disinfection Effectiveness on SARS-CoV-2 Transmission in Indoor Environment Peer-reviewed

Syun-suke Kadoya, Sewwandi Bandara, Masayuki Ogata, Takayuki Miura, Michiko Bando, Daisuke Sano

Infectious Disease Modelling　2024/10
Publisher: Elsevier BV
DOI： 10.1016/j.idm.2024.10.004 　

ISSN： 2468-0427
A soft-sensor approach for predicting an indicator virus removal efficiency of a pilot-scale anaerobic membrane bioreactor (AnMBR) Peer-reviewed

Syun-suke Kadoya, Yifan Zhu, Rong Chen, Chao Rong, Yuyou Li, Daisuke Sano

Journal of Water and Health　2024/05/25
Publisher: IWA Publishing
DOI： 10.2166/wh.2024.251 　

ISSN： 1477-8920

eISSN： 1996-7829

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ABSTRACT <p></p> The anaerobic membrane bioreactor (AnMBR) is a promising technology for not only water reclamation but also virus removal; however, the virus removal efficiency of AnMBR has not been fully investigated. Additionally, the removal efficiency estimation requires datasets of virus concentration in influent and effluent, but its monitoring is not easy to perform for practical operation because the virus quantification process is generally time-consuming and requires specialized equipment and trained personnel. Therefore, in this study, we aimed to identify the key, monitorable variables in AnMBR and establish the data-driven models using the selected variables to predict virus removal efficiency. We monitored operational and environmental conditions of AnMBR in Sendai, Japan and measured virus concentration once a week for six months. Spearman's rank correlation analysis revealed that the pH values of influent and mixed liquor suspended solids (MLSS) were strongly correlated with the log reduction value of pepper mild mottle virus, indicating that electrostatic interactions played a dominant role in AnMBR virus removal. Among the candidate models, the random forest model using selected variables including influent and MLSS pH outperformed the others. This study has demonstrated the potential of AnMBR as a viable option for municipal wastewater reclamation with high microbial safety.
Pepper mild mottle virus intended for use as a process indicator for drinking water treatment: present forms and quantitative relations to norovirus and rotavirus in surface water Peer-reviewed

Takayuki Miura, Syun-suke Kadoya, Yohei Miura, Hiroyuki Takino, Michihiro Akiba, Daisuke Sano, Takanori Masuda

Water Research　257　121713　2024/05

DOI： 10.1016/j.watres.2024.121713 　
Multiple pathogen contamination of water, hands, and fomites in rural Nepal and the effect of WaSH interventions. International-journal Peer-reviewed

Sital Uprety, Isaac Ngo, Marika Maggos, Bipin Dangol, Samendra P Sherchan, Joanna L Shisler, Mohan Amarasiri, Daisuke Sano, Thanh H Nguyen

International journal of hygiene and environmental health　257　114341-114341　2024/04
Publisher: Elsevier BV
DOI： 10.1016/j.ijheh.2024.114341 　

ISSN： 1438-4639

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Water, Sanitation, and Hygiene (WaSH) interventions are the most effective in reducing diarrheal disease severity and prevalence. However, very few studies have investigated the effectiveness of WaSH intervention in reducing pathogen presence and concentration. In this study, we employed a microfluidic PCR approach to quantify twenty bacterial pathogens in water (n = 360), hands (n = 180), and fomite (n = 540) samples collected in rural households of Nepal to assess the pathogen exposures and the effect of WaSH intervention on contamination and exposure rates. The pathogen load and the exposure pathways for each pathogen in intervention and control villages were compared to understand the effects of WaSH intervention. Pathogens were detected in higher frequency and concentration from fomites samples, toilet handle (21.42%; 5.4,0 95%CI: mean log10 of 4.69, 5.96), utensils (23.5%; 5.47, 95%CI: mean log10 of 4.77, 6.77), and water vessels (22.42%; 5.53, 95%CI: mean log10 of 4.79, 6.60) as compared to cleaning water (14.36%; 5.05, 95%CI: mean log10 of 4.36, 5.89), drinking water (14.26%; 4.37, 85%CI: mean log10 of 4.37, 5.87), and hand rinse samples (16.92%; 5.49, 95%CI: mean log10 of 4.77, 6.39). There was no clear evidence that WaSH intervention reduced overall pathogen contamination in any tested pathway. However, we observed a significant reduction (p < 0.05) in the prevalence, but not concentration, of some target pathogens, including Enterococcus spp. in the intervention village compared to the control village for water and hands rinse samples. Conversely, no significant reduction in target pathogen concentration was observed for water and hand rinse samples. In swab samples, there was a reduction mostly in pathogen concentration rather than pathogen prevalence, highlighting that a reduction in pathogen prevalence was not always accompanied by a reduction in pathogen concentration. This study provides an understanding of WaSH intervention on microbe concentrations. Such data could help with better planning of intervention activities in the future.
Increased faecal shedding in SARS-CoV-2 variants BA.2.86 and JN.1. International-journal International-coauthorship Peer-reviewed

Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Puey Ounjai, Andrew C Singer, Naveen Kumar Devanga Ragupathi, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Tanittha Chatsuwan, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Richard Siow, Sam Trowsdale, Parichart Hongsing, Aisha Khatib, Kenji Shibuya, Shuichi Abe, Hitoshi Ishikawa

The Lancet. Infectious diseases　2024/03/21

DOI： 10.1016/S1473-3099(24)00155-5 　
Exploring indoor and outdoor dust as a potential tool for detection and monitoring of COVID-19 transmission. International-journal International-coauthorship Peer-reviewed

Suparinthon Anupong, Sudarat Chadsuthi, Parichart Hongsing, Cameron Hurst, Phatthranit Phattharapornjaroen, Ali Hosseini Rad S M, Stefan Fernandez, Angkana T Huang, Porames Vatanaprasan, Thammakorn Saethang, Sirirat Luk-In, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Natharin Ngamwongsatit, Vishnu Nayak Badavath, Wanwara Thuptimdang, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Kazuhiko Miyanaga, Longzhu Cui, Asuka Nanbo, Kenji Shibuya, Rosalyn Kupwiwat, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Paul G Higgins, Anthony Kicic, Andrew C Singer, Tanittha Chatsuwan, Sam Trowsdale, Shuichi Abe, Hitoshi Ishikawa, Mohan Amarasiri, Charin Modchang, Dhammika Leshan Wannigama

iScience　27　(3)　109043-109043　2024/03/15

DOI： 10.1016/j.isci.2024.109043 　

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This study investigated the potential of using SARS-CoV-2 viral concentrations in dust as an additional surveillance tool for early detection and monitoring of COVID-19 transmission. Dust samples were collected from 8 public locations in 16 districts of Bangkok, Thailand, from June to August 2021. SARS-CoV-2 RNA concentrations in dust were quantified, and their correlation with community case incidence was assessed. Our findings revealed a positive correlation between viral concentrations detected in dust and the relative risk of COVID-19. The highest risk was observed with no delay (0-day lag), and this risk gradually decreased as the lag time increased. We observed an overall decline in viral concentrations in public places during lockdown, closely associated with reduced human mobility. The effective reproduction number for COVID-19 transmission remained above one throughout the study period, suggesting that transmission may persist in locations beyond public areas even after the lockdown measures were in place.
Wastewater-based epidemiological surveillance of SARS-CoV-2 new variants BA.2.86 and offspring JN.1 in south and Southeast Asia. International-journal International-coauthorship Peer-reviewed

Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, W K C P Werawatte, S M Ali Hosseini Rad, Stefan Fernandez, Angkana T Huang, Porames Vatanaprasan, Thammakorn Saethang, Sirirat Luk-In, Robin James Storer, Puey Ounjai, Ratana Tacharoenmuang, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Bernadina Cynthia, John Jefferson V Besa, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Andrew C Singer, Tanittha Chatsuwan, Sam Trowsdale, Takashi Furukawa, Kazunari Sei, Daisuke Sano, Hitoshi Ishikawa, Kenji Shibuya, Aisha Khatib, Shuichi Abe, Parichart Hongsing

Journal of travel medicine　2024/03/04

DOI： 10.1093/jtm/taae040 　
Stochastic Dual Coordinate Ascent for Learning Sign Constrained Linear Predictors

Yuya TAKADA, Rikuto MOCHIDA, Miya NAKAJIMA, Syun-suke KADOYA, Daisuke SANO, Tsuyoshi KATO

IEICE Transactions on Information and Systems　2024
Publisher: Institute of Electronics, Information and Communications Engineers (IEICE)
DOI： 10.1587/transinf.2023edp7139 　

ISSN： 0916-8532

eISSN： 1745-1361
Genetic diversity of murine norovirus populations less susceptible to chlorine Peer-reviewed

Aken Puti Wanguyun, Wakana Oishi, Andri Taruna Rachmadi, Kazuhiko Katayama, Daisuke Sano

Frontiers in Microbiology　15　2024

DOI： 10.3389/fmicb.2024.1372641 　

eISSN： 1664-302X
Prediction of algal bloom using a combination of sparse modeling and a machine learning algorithm: Automatic relevance determination and support vector machine Peer-reviewed

Yohei Miura, Hiroomi Imamoto, Yasuhiro Asada, Masaki Sagehashi, Michihiro Akiba, Osamu Nishimura, Daisuke Sano

Ecological Informatics　78　2023/12

DOI： 10.1016/j.ecoinf.2023.102337 　

ISSN： 1574-9541
Tracing the new SARS-CoV-2 variant BA.2.86 in the community through wastewater surveillance in Bangkok, Thailand. International-journal International-coauthorship Peer-reviewed

Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T Huang, Puey Ounjai, Ratana Tacharoenmuang, Naveen Kumar Devanga Ragupathi, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Andrew C Singer, Tanittha Chatsuwan, Sam Trowsdale, Aisha Khatib, Kenji Shibuya, Shuichi Abe, Hitoshi Ishikawa, Parichart Hongsing

The Lancet Infectious Diseases　23　(11)　e464-e466　2023/10/06

DOI： 10.1016/S1473-3099(23)00620-5 　
Tracing the transmission of mpox through wastewater surveillance in Southeast Asia. International-journal International-coauthorship Peer-reviewed

Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, Wanwara Thuptimdang, S M Ali Hosseini Rad, Stefan Fernandez, Angkana T Huang, Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-In, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G Higgins, Asuka Nanbo, Anthony Kicic, Andrew C Singer, Tanittha Chatsuwan, Sam Trowsdale, Richard Siow, Kenji Shibuya, Shuichi Abe, Hitoshi Ishikawa, Parichart Hongsing

Journal of travel medicine　30　(5)　2023/09/05

DOI： 10.1093/jtm/taad096 　

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High population density and tourism in Southeast Asia increase the risk of mpox due to frequent interpersonal contacts. Our wastewater surveillance in six Southeast Asian countries revealed positive signals for Monkeypox virus (MPXV) DNA, indicating local transmission. This alerts clinicians and helps allocate resources like testing, vaccines and therapeutics in resource-limited countries.
COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities Peer-reviewed

Dhammika Leshan Wannigama, Mohan Amarasiri, Parichart Hongsing, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Phatthranit Phattharapornjaroen, Ali Hosseini Rad S.M., Stefan Fernandez, Angkana T. Huang, Porames Vatanaprasan, Dylan John Jay, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Nattiya Hirankarn, Paul G. Higgins, Anthony Kicic, Andrew C. Singer, Tanittha Chatsuwan, Sam Trowsdale, Shuichi Abe, Alexander D. McLellan, Hitoshi Ishikawa

iScience　26　(7)　107019-107019　2023/06
Publisher: Elsevier BV
DOI： 10.1016/j.isci.2023.107019 　

ISSN： 2589-0042
Microbial assessment of water, sanitation, and hygiene (WaSH) in temporary and permanent settlements two years after Nepal 2015 earthquake Peer-reviewed

Sital Uprety, Samendra P. Sherchan, Preeti Narayanan, Bipin Dangol, Marika Maggos, Alex Celmer, Joanna Shisler, Mohan Amarasiri, Daisuke Sano, Thanh H. Nguyen

Science of The Total Environment　877　162867-162867　2023/06
Publisher: Elsevier BV
DOI： 10.1016/j.scitotenv.2023.162867 　

ISSN： 0048-9697
Decay rate estimation of respiratory viruses in aerosols and on surfaces under different environmental conditions Peer-reviewed

Sewwandi Bandara, Wakana Oishi, Syun-suke Kadoya, Daisuke Sano

International Journal of Hygiene and Environmental Health　251　114187-114187　2023/06
Publisher: Elsevier BV
DOI： 10.1016/j.ijheh.2023.114187 　

ISSN： 1438-4639
Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia Peer-reviewed

Dhammika Leshan Wannigama, Mohan Amarasiri, Parichart Hongsing, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Phatthranit Phattharapornjaroen, Ali Hosseini Rad S.M., Stefan Fernandez, Angkana T. Huang, Naris Kueakulpattana, Chanikan Tanasatitchai, Porames Vatanaprasan, Thammakorn Saethang, Sirirat Luk-in, Robin James Storer, Puey Ounjai, Naveen Kumar Devanga Ragupathi, Phitsanuruk Kanthawee, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Nattiya Hirankarn, Paul G. Higgins, Anthony Kicic, Tanittha Chatsuwan, Alexander D. McLellan, Shuichi Abe

Science of The Total Environment　858　159816-159816　2023/02
Publisher: Elsevier BV
DOI： 10.1016/j.scitotenv.2022.159816 　

ISSN： 0048-9697
Estimation of alkali dosage and contact time for treating human excreta containing viruses as an emergency response: a systematic review Peer-reviewed

Wakana Oishi, Daisuke Sano

Frontiers in Public Health　11　2023

DOI： 10.3389/fpubh.2023.1286595 　

eISSN： 2296-2565
Temporal variations of human and animal Rotavirus A genotypes in surface water used for drinking water production Peer-reviewed

Takayuki Miura, Syun-suke Kadoya, Hiroyuki Takino, Daisuke Sano, Michihiro Akiba

Frontiers in Microbiology　13　2022/08/09
Publisher: Frontiers Media SA
DOI： 10.3389/fmicb.2022.912147 　

eISSN： 1664-302X

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Rotavirus is a major cause of gastroenteritis among infants and children. In this study, nested PCR assays were developed to amplify partial regions of the VP7, VP4, and VP6 genes of Rotavirus A (RVA) for amplicon-based Illumina MiSeq sequencing to investigate RVA genotypes in environmental water samples. Eight sets of inner primers were first designed and screened for use in the nested PCR assays, and four sets of them could produce amplicons. Six sets of outer primers were then designed and combined with the four sets of inner primers that worked. The assays were evaluated for sensitivity using raw water samples collected from one drinking water treatment plant between April 2019 and March 2020 (Sample Set 1; N = 12) and seven DWTPs between 2018 and 2020 (Sample Set 2; N = 18). In total, 43 amplicons from Set 1 were sequenced and diverse sequences from human, porcine, bovine, equine, and feline RVA were observed. Human G8, G3, and G2 genotypes were obtained, with G8 predominant (relative abundance, 36–87%) in samples taken during the rotavirus epidemic season between April and June. Porcine G5, G11, and G4, and bovine G10 and G6 genotypes were also detected. VP4 sequence analysis revealed that the human P[8] genotype was present throughout the year, whereas P[4] and P[9] were present only in the epidemic season. The vaccine strains P[5] and P[8] (RotaTeq^®) were also detected. Our approach enables the identification of prevalent human and animal RVA genotypes and their host species that potentially caused fecal contamination in water sources.
Unveiling the characterization and development of prokaryotic community during the start-up and long-term operation of a pilot-scale anaerobic membrane bioreactor for the treatment of real municipal wastewater Peer-reviewed

Zhe Kong, Lu Li, Jiang Wu, Chao Rong, Tianjie Wang, Rong Chen, Daisuke Sano, Yu-You Li

Science of The Total Environment　813　152643-152643　2022/03
Publisher: Elsevier BV
DOI： 10.1016/j.scitotenv.2021.152643 　

ISSN： 0048-9697

eISSN： 1879-1026
COVID-19 case prediction via wastewater surveillance in a low-prevalence urban community: a modeling approach. International-journal Peer-reviewed

Yifan Zhu, Wakana Oishi, Chikako Maruo, Sewwandi Bandara, Mu Lin, Mayuko Saito, Masaaki Kitajima, Daisuke Sano

Journal of water and health　20　(2)　459-470　2022/02

DOI： 10.2166/wh.2022.183 　

ISSN： 1477-8920

eISSN： 1996-7829
Comparison of five polyethylene glycol precipitation procedures for the RT-qPCR based recovery of murine hepatitis virus, bacteriophage phi6, and pepper mild mottle virus as a surrogate for SARS-CoV-2 from wastewater International-journal Peer-reviewed

Shotaro Torii, Wakana Oishi, Yifan Zhu, Ocean Thakali, Bikash Malla, Zaizhi Yu, Bo Zhao, Chisato Arakawa, Masaaki Kitajima, Akihiko Hata, Masaru Ihara, Shigeru Kyuwa, Daisuke Sano, Eiji Haramoto, Hiroyuki Katayama

Science of The Total Environment　807　(Pt 2)　150722-150722　2022/02
Publisher: Elsevier BV
DOI： 10.1016/j.scitotenv.2021.150722 　

ISSN： 0048-9697

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Polyethylene glycol (PEG) precipitation is one of the conventional methods for virus concentration. This technique has been used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater. The procedures and seeded surrogate viruses were different among implementers; thus, the reported whole process recovery efficiencies considerably varied among studies. The present study compared five PEG precipitation procedures, with different operational parameters, for the RT-qPCR-based whole process recovery efficiency of murine hepatitis virus (MHV), bacteriophage phi6, and pepper mild mottle virus (PMMoV), and molecular process recovery efficiency of murine norovirus using 34 raw wastewater samples collected in Japan. The five procedures yielded significantly different whole process recovery efficiency of MHV (0.070%-2.6%) and phi6 (0.071%-0.51%). The observed concentration of indigenous PMMoV ranged from 8.9 to 9.7 log (8.2 × 108 to 5.6 × 109) copies/L. Interestingly, PEG precipitation with 2-h incubation outperformed that with overnight incubation partially due to the difference in molecular process recovery efficiency. The recovery load of MHV exhibited a positive correlation (r = 0.70) with that of PMMoV, suggesting that PMMoV is the potential indicator of the recovery efficiency of SARS-CoV-2. In addition, we reviewed 13 published studies and found considerable variability between different studies in the whole process recovery efficiency of enveloped viruses by PEG precipitation. This was due to the differences in operational parameters and surrogate viruses as well as the differences in wastewater quality and bias in the measurement of the seeded load of surrogate viruses, resulting from the use of different analytes and RNA extraction methods. Overall, the operational parameters (e.g., incubation time and pretreatment) should be optimized for PEG precipitation. Co-quantification of PMMoV may allow for the normalization of SARS-CoV-2 RNA concentration by correcting for the differences in whole process recovery efficiency and fecal load among samples.
PROJECTING VIRUS INACTIVATION EFFICIENCY IN CHLORINE DISINFECTION TOWARDS HYGIENIC SAFETY MANAGEMENT OF WASTEWATER EFFLUENT WITH AN ENVIRONMENAL PREDICTIVE MICROBIOLOGICAL APPROACH

Atsuhiro ISHII, Wakana OISHI, Syun-suke KADOYA, Daisuke SANO

Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research)　78　(7)　III_11-III_21　2022
Publisher: Japan Society of Civil Engineers
DOI： 10.2208/jscejer.78.7_iii_11 　

eISSN： 2185-6648
The Intrapopulation Genetic Diversity of RNA Virus May Influence the Sensitivity of Chlorine Disinfection. International-journal Peer-reviewed

Syun-Suke Kadoya, Syun-Ichi Urayama, Takuro Nunoura, Miho Hirai, Yoshihiro Takaki, Masaaki Kitajima, Toyoko Nakagomi, Osamu Nakagomi, Satoshi Okabe, Osamu Nishimura, Daisuke Sano

Frontiers in microbiology　13　839513-839513　2022

DOI： 10.3389/fmicb.2022.839513 　

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RNA virus populations are not clonal; rather, they comprise a mutant swarm in which sequences are slightly different from the master sequence. Genetic diversity within a population (intrapopulation genetic diversity) is critical for RNA viruses to survive under environmental stresses. Disinfection has become an important practice in the control of pathogenic viruses; however, the impact of intrapopulation genetic diversity on the sensitivity of disinfection, defined as -log10 (postdisinfected infectious titer/predisinfected titer), has not been elucidated. In this study, we serially passaged populations of rhesus rotavirus. We demonstrated that populations with reduced chlorine sensitivity emerged at random and independently of chlorine exposure. Sequencing analysis revealed that compared with sensitive populations, less-sensitive ones had higher non-synonymous genetic diversity of the outer capsid protein gene, suggesting that changes in the amino acid sequences of the outer capsid protein were the main factors influencing chlorine sensitivity. No common mutations were found among less-sensitive populations, indicating that rather than specific mutations, the diversity of the outer capsid protein itself was associated with the disinfection sensitivity and that the disinfection sensitivity changed stochastically. Simulation results suggest that the disinfection sensitivity of a genetically diverse population is destabilized if cooperative viral clusters including multiple sequences are formed. These results advocate that any prevention measures leading to low intrapopulation genetic diversity are important to prevent the spread and evolution of pathogenic RNA viruses in society.
Experimental Adaptation of Murine Norovirus to Calcium Hydroxide. International-journal Peer-reviewed

Wakana Oishi, Mikiko Sato, Kengo Kubota, Ryoka Ishiyama, Reiko Takai-Todaka, Kei Haga, Kazuhiko Katayama, Daisuke Sano

Frontiers in microbiology　13　848439-848439　2022

DOI： 10.3389/fmicb.2022.848439 　

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Slaked lime (calcium hydroxide) is a commonly used disinfectant for fecal sludge. Although viruses are inactivated by lime treatment, whether RNA viruses adapt to lime treatment has not yet been determined. Here, we show that murine norovirus developed higher tolerance during serial passages with lime treatment. We compared synonymous and non-synonymous nucleotide diversities of the three open reading frames of viral genome and revealed that virus populations were subjected to enhanced purifying selection over the course of serial passages with lime treatment. Virus adaptation to lime treatment was coincident with amino acid substitution of lysine to arginine at position 345 (K345R) on the major capsid protein VP1, which accounted for more than 90% of the population. The infectious clones with the K345R produced using a plasmid-based reverse genetics system exhibited greater tolerance in a lime solution, which indicated that the specific amino acid substitution was solely involved in the viral tolerance in lime treatment.
Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance Peer-reviewed

Warish Ahmed, Stuart L. Simpson, Paul M. Bertsch, Kyle Bibby, Aaron Bivins, Linda L. Blackall, Sílvia Bofill-Mas, Albert Bosch, João Brandão, Phil M. Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas H. Farnleitner, Daniel Gerrity, Raul Gonzalez, John F. Griffith, Pradip Gyawali, Charles N. Haas, Kerry A. Hamilton, Hapuarachchige Chanditha Hapuarachchi, Valerie J. Harwood, Rehnuma Haque, Greg Jackson, Stuart J. Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe A. Layton, Erin Lipp, Sandra L. McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim G. Meijer, Jochen F. Mueller, Heather Murphy, Coleen C. Naughton, Rachel T. Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkänen, Veronica B. Rajal, Brandon Reyneke, Fernando A. Roman, Joan B. Rose, Marta Rusiñol, Michael J. Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra P. Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua A. Steele, Rosalie Sabburg, Erin M. Symonds, Phong Thai, Kevin V. Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy S. Whiteley, Judith Chui Ching Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity G. Zimmer-Faust, Orin C. Shanks

Science of The Total Environment　805　149877-149877　2022/01
Publisher: Elsevier BV
DOI： 10.1016/j.scitotenv.2021.149877 　

ISSN： 0048-9697
Disinfection efficacy and mechanism of olanexidine gluconate against norovirus Peer-reviewed

Kaoru Imai, Makoto Tanaka, Seiji Miyoshi, Ren Murakami, Akifumi Hagi, Sachi Yamagawa, Daisuke Sano

American Journal of Infection Control　2021/12
Publisher: Elsevier BV
DOI： 10.1016/j.ajic.2021.11.020 　

ISSN： 0196-6553
Predictive Environmental Microbiology for Safe Use of Sanitation Products in Agriculture: Challenges and Perspectives Peer-reviewed

Wakana Oishi, Björn Vinnerås, Joan B. Rose, Daisuke Sano

Environmental Science & Technology Letters　8　(11)　924-931　2021/11/09
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.estlett.1c00537 　

ISSN： 2328-8930

eISSN： 2328-8930
The water temperature changes the effect of pH on copper toxicity to the green microalgae Raphidocelis subcapitata Peer-reviewed

Gissela Pascual, Daisuke Sano, Takashi Sakamaki, Michihiro Akiba, Osamu Nishimura

Chemosphere　133110-133110　2021/11
Publisher: Elsevier BV
DOI： 10.1016/j.chemosphere.2021.133110 　

ISSN： 0045-6535
Wastewater-based Epidemiology for Infectious Diseases: The Foundations and Future Perspectives Peer-reviewed

大石若菜, ZHU Yifan, 丸尾知佳子, 斉藤繭子, 北島正章, 佐野大輔, 佐野大輔

水環境学会誌(Web)　44　(5)　125-133　2021/09

ISSN： 1881-3690
Early warning of COVID-19 in Tokyo via wastewater-based epidemiology: How feasible it really is? Peer-reviewed

Yifan Zhu, Wakana Oishi, Mayuko Saito, Masaaki Kitajima, Daisuke Sano

Journal of Water and Environment Technology　19　(3)　170-183　2021/06
Publisher: Japan Society on Water Environment
DOI： 10.2965/jwet.21-024 　

eISSN： 1348-2165
Olanexidine gluconate formulations as environmental disinfectants for enveloped viruses infection control Peer-reviewed

Kaoru Imai, Akifumi Hagi, Sachi Yamagawa, Daisuke Sano

Journal of Hospital Infection　112　37-41　2021/06
Virus disinfection and population genetics: Toward the control of waterborne virus diseases by water engineering Peer-reviewed

Syun-suke Kadoya, Hiroyuki Katayama, Daisuke Sano

Current Pollution Reports　7　407-416　2021/05
Predictive water virology using regularized regression analyses for projecting virus inactivation efficiency in ozone disinfection Peer-reviewed

Syun-suke Kadoya, Osamu Nishimura, Hiroyuki Kato, Daisuke Sano

Water Research X　11　100093　2021/05

DOI： 10.1016/j.wroa.2021.100093 　

eISSN： 2589-9147
Early warning of COVID-19 via wastewater-based epidemiology: potential and bottlenecks International-journal Peer-reviewed

Yifan Zhu, Wakana Oishi, Chikako Maruo, Mayuko Saito, Rong Chen, Masaaki Kitajima, Daisuke Sano

Science of the Total Environment　767　145124-145124　2021/05

DOI： 10.1016/j.scitotenv.2021.145124 　

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An effective early warning tool is of great administrative and social significance to the containment and control of an epidemic. Facing the unprecedented global public health crisis caused by COVID-19, wastewater-based epidemiology (WBE) has been given high expectations as a promising surveillance complement to clinical testing which had been plagued by limited capacity and turnaround time. In particular, recent studies have highlighted the role WBE may play in being a part of the early warning system. In this study, we briefly discussed the basics of the concept, the benefits and critical points of such an application, the challenges faced by the scientific community, the progress made so far, and what awaits to be addressed by future studies to make the concept work. We identified that the shedding dynamics of infected individuals, especially in the form of a mathematical shedding model, and the back-calculation of the number of active shedders from observed viral load are the major bottlenecks of WBE application in the COVID-19 pandemic that deserve more attention, and the sampling strategy (location, timing, and interval) needs to be optimized to fit the purpose and scope of the WBE project.
Hierarchical Bayesian modeling for predictive environmental microbiology toward a safe use of human excreta: Systematic review and meta-analysis Peer-reviewed

Wakana Oishi, Syun-suke Kadoya, Osamu Nishimura, Joan B. Rose, Daisuke Sano

Journal of Environmental Management　284　112088　2021/04

DOI： 10.1016/j.jenvman.2021.112088 　

ISSN： 0301-4797

eISSN： 1095-8630
Biochar and GAC intensify anaerobic phenol degradation via distinctive adsorption and conductive properties Peer-reviewed

Qian Li, Xin Gao, Yaqian Liu, Gaojun Wang, Yu-You Li, Daisuke Sano, Xiaochang Wang, Rong Chen

Journal of Hazardous Materials　124183-124183　2021/03
Publisher: Elsevier BV
DOI： 10.1016/j.jhazmat.2020.124183 　

ISSN： 0304-3894
Human norovirus disease burden of consuming Crassostrea gigas oysters: A case-study from Japan Peer-reviewed

Yo Ueki, Mohan Amarasiri, Sayaka Kamio, Akie Sakagami, Hiroshi Ito, Sital Uprety, Arief Nurul Umam, Takayuki Miura, Thanh H. Nguyen, Daisuke Sano

Food Control　121　107556-107556　2021/03
Publisher: Elsevier BV
DOI： 10.1016/j.foodcont.2020.107556 　

ISSN： 0956-7135
Corrigendum to “Effects of chemical interaction of nutrients and EDTA on metals toxicity to Pseudokirckneriella subcapitata” [Ecotoxicol. Environ. Saf. 203 (2020) 110966] (Ecotoxicology and Environmental Safety (2020) 203, (S0147651320308058), (10.1016/j.ecoenv.2020.110966))

Gissela Pascual, Daisuke Sano, Takashi Sakamaki, Osamu Nishimura

Ecotoxicology and Environmental Safety　208　2021/01/15

DOI： 10.1016/j.ecoenv.2020.111465 　

ISSN： 0147-6513

eISSN： 1090-2414
Relationship between Viral Infections, Routes of Transmission to Humans and Infection Control-To Prevent Airborne Transmission-

TAKATSUKA Takeshi, UDAGAWA Yohichi, KITAJIMA Masaaki, SANO Daisuke, UTAGAWA Etsuko

Transactions of the Society of Heating,Air-conditioning and Sanitary Engineers of Japan　46　(286)　55-58　2021/01/05
Publisher: The Society of Heating, Air-Conditioning & Sanitary Engineers of Japan
DOI： 10.18948/shase.46.286_55 　

ISSN： 0385-275X

eISSN： 2424-0486

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Influenza viruses and noroviruses are the most common causes of respiratory and gastrointestinal infections. Viruses are tiny protein particles that cannot self-propagate, but they carry genetic materials (DNA and RNA) that can negatively affect humans if they infect them. Viruses are small in size (30-300 nm) and can reach deep into the respiratory tract and into the alveoli. On the contrary, bacteria as well as viruses may also affect the severity of the disease after virus infection. This report summarizes the characteristics of viruses, the route of infection, humidity and airflow around a toilet.
Wastewater-based Epidemiology for Infectious Diseases: The Foundations and Future Perspectives

OISHI Wakana, ZHU Yifan, MARUO Chikako, SAITO Mayuko, KITAJIMA Masaaki, SANO Daisuke

Journal of Japan Society on Water Environment　44　(5)　125-133　2021
Publisher: Japan Society on Water Environment
DOI： 10.2965/jswe.44.125 　

ISSN： 0916-8958

eISSN： 1881-3690

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Wastewater-base epidemiology may be a promising approach to capturing the dynamics of the spread of diseases as well as emerging mutants in a catchment. Herein, we describe the key components of the wastewater-based epidemiology of viral infectious diseases, including the optimization of the methodology of the detection and quantification of viral genomes and the development of a back-calculation model to estimate the number of infected individuals. Early warning based on the influent viral concentration in a wastewater treatment plant may be feasible when it takes longer than one week to tally the number of patients of the target infectious disease by the currently used sentinel surveillance under the infectious diseases control law. The variables of a back-calculation model are categorized into two types: common variables irrespective of the type of infectious disease and variables specific to the infectious disease including shedding profiles of viruses. Greater effort is required to develop a robust back-calculation model against variability and uncertainty due to environmental, geological, and personal differences.
Viral Interference as a Factor of False-Negative in the Infectious Adenovirus Detection Using Integrated Cell Culture-PCR with a BGM Cell Line Peer-reviewed

Daisuke Sano, Ryosuke Watanabe, Wakana Oishi, Mohan Amarasiri, Masaaki Kitajima, Satoshi Okabe

Food and Environmental Virology　13　84-91　2021/01
Virus removal by membrane bioreactors: A review of mechanism investigation and modeling efforts Peer-reviewed

Yifan Zhu, Rong Chen, Yu-You Li, Daisuke Sano

Water Research　188　116522-116522　2021/01
Publisher: Elsevier BV
DOI： 10.1016/j.watres.2020.116522 　

ISSN： 0043-1354
スパース推定法と階層ベイズ推定法による環境水中ウイルス自然死滅モデルの構築 Peer-reviewed

大石若菜, 加藤郁生, 西村修, 佐野大輔

土木学会論文集　76　(6)　III_449-III_460　2020/12
Updated research agenda for water, sanitation and antimicrobial resistance Peer-reviewed

Daisuke Sano, Astrid Louse Wester, Heike Schmitt, Mohan Amarasiri, Amy Kirby, Kate Medlicott, Ana Maria de, Roda Husman

Journal of Water and Health　18　(6)　858-868　2020/12
The Effect of GD1a Ganglioside-Expressing Bacterial Strains on Murine Norovirus Infectivity International-journal Peer-reviewed

Yifan Zhu, Hiroki Kawai, Satoshi Hashiba, Mohan Amarasiri, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano

Molecules　25　(18)　4084-4084　2020/09/07
Publisher: MDPI AG
DOI： 10.3390/molecules25184084 　

eISSN： 1420-3049

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In this study, we investigated the impact of GD1a-expressing bacterial strains on the infectivity of murine norovirus (MNV). Eligible bacterial strains were screened from a sewage sample using flow cytometry, and their genetic sequences of 16S rRNA were determined. The enzyme-linked immunosorbent assay (ELISA) was employed to analyze the binding between bacteria and MNV particles, and the plaque assay was used to assess the effects of GD1a-positive and negative strains on MNV infectivity. The result from ELISA shows that MNV particles are able to bind to both GD1a-positive and negative bacterial strains, but the binding to the GD1a-positive strain is more significant. The infectivity assay result further shows that the MNV infectious titer declined with an increasing concentration of GD1a-positive bacteria. The addition of anti-GD1a antibody in the infectivity assay led to the recovery of the MNV infectious titer, further confirming that the binding between MNV particles and bacterial GD1a ganglioside compromises MNV infectivity. Our findings highlight the role indigenous bacteria may play in the lifecycle of waterborne enteric viruses as well as the potential of exploiting them for virus transmission intervention and water safety improvement.
Inactivation kinetics modeling of Escherichia coli in concentrated urine for implementing predictive environmental microbiology in sanitation safety planning. International-journal Peer-reviewed

Wakana Oishi, Ikuo Kato, Nowaki Hijikata, Ken Ushijima, Ryusei Ito, Naoyuki Funamizu, Osamu Nishimura, Daisuke Sano

Journal of Environmental Management　268　110672-110672　2020/08/15

DOI： 10.1016/j.jenvman.2020.110672 　

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Urine concentration (condensation) leads to the inactivation of pathogens in urine owing to a hyperosmotic environment. This study proposed an inactivation kinetic model of Escherichia coli (E. coli), a surrogate of human bacterial pathogens, in concentrated synthetic urine. The model parameters were obtained under an assumption that the inactivation rate of E. coli followed a binomial distribution, which made it possible to accurately simulate the time-course decay of E. coli in synthetic urine. The inactivation rate constant values obtained in concentrated urine samples, ammonium buffer solutions and carbonate buffer solutions indicated that the osmotic pressure was a relatively predominant cause for the inactivation of E. coli. The appropriate storage time was estimated using the approach of quantitative microbial risk assessment, which indicated that the 5-fold concentrated urine could be safely collected after 1-day storage when urea was hydrolyzed, whereas 91-hour storage was required for non-concentrated urine. The occupational risk was not negligible even with 6-month storage at 20 °C when urea was not hydrolyzed, which suggested that the urine storage styles should be clarified more minutely. The present study highlights the importance of "predictive environmental microbiology," which deals with inactivation kinetic models of microorganisms under varied environmental conditions to fully implement the hazard analysis and critical control point (HACCP) approach for the safe use of human excreta in agriculture.
Identification of novel norovirus polymerase genotypes from pediatric fecal samples collected between the year 1997 and 2000 in Japan. International-journal Peer-reviewed

Mohan Amarasiri, Etsuko Utagawa, Daisuke Sano, Kazuhiko Katayama

Infection, Genetics and Evolution　82　104313-104313　2020/08

DOI： 10.1016/j.meegid.2020.104313 　

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We analyzed 46 pediatric fecal samples collected between the years 1997 and 2000 to retrospectively evaluate the norovirus strains circulating during that era and to identify possible re-emergence patterns. From the tested fecal samples, we detected GII.1, GII.3, GII.4 (95/96-US) and GII.6 strains. Most importantly, two novel polymerase genotypes (GI.PNA4 and GII.PNA7) were detected during the study. Two possible recombinant strains (GII.6[P7] and GII.3[P29]) were identified and SimPlot analysis confirmed that GII.6[P7] is a recombinant strain. The study emphasizes the importance of retrospective evaluation of human fecal samples in obtaining a better understanding of norovirus circulation, re-emergence and evolution.
Effects of chemical interaction of nutrients and EDTA on metals toxicity to Pseudokirckneriella subcapitata. International-journal Peer-reviewed

Gissela Pascual, Daisuke Sano, Takashi Sakamaki, Osamu Nishimura

Ecotoxicology and Environmental Safety　203　110966-110966　2020/07/14
Publisher: Elsevier BV
DOI： 10.1016/j.ecoenv.2020.110966 　

ISSN： 0147-6513

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We studied the effect of the chemical interaction of nutrients and the ethylenediamine tetraacetic acid (EDTA) on metals toxicity. Growth inhibition tests of Pseudokirchneriella subcapitata by nutrient metals copper (Cu) and zinc (Zn), and the non-nutrient metal lead (Pb), were performed. The high-enriched Bold's Basal medium (BBm) and two low-enriched standard media, recommended by the Organization for Economic Cooperation and Development (OECDm) and Environmental Protection Agency-algal assay procedure medium (AAPm), were used in this study. The metals toxicity was affected by the interaction of nutrients and EDTA. Cu+2 was more toxic in the OECDm (EC50 20.3 μg/L), while Pb+2 (EC50 23.1 μg/L) and Zn+2 (EC50 99.4 μg/L) in the AAPm. Non-toxic effect of these metals was observed in BBm, but the exclusion of EDTA shifted it into a toxic medium. Finally, we found that the toxicity of the studied nutrient metals is mainly influenced by EDTA, which reduced the concentration of ionized metals, while the toxicity of the non-nutrient metal is affected by EDTA and phosphates.
Virucidal efficacy of olanexidine gluconate as a hand antiseptic against human norovirus International-journal Peer-reviewed

Kaoru Imai, Akifumi Hagi, Yasuhide Inoue, Mohan Amarasiri, Daisuke Sano

Food and Environmental Virology　12　(2)　180-190　2020/06
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s12560-020-09422-4 　

ISSN： 1867-0334

eISSN： 1867-0342

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Human noroviruses are the major cause of non-bacterial acute gastroenteritis worldwide. Since no therapeutic agent has been proven to prevent human norovirus infection yet, preventive healthcare interventions to block the infection routes play an important role in infection control. One of the possible infection routes of human noroviruses are through contaminated hands, but no hand antiseptics have been proven effective. Olanexidine gluconate is a new biguanide compound that has already been approved for sale as an antiseptic for the surgical field in Japan. A new hand antiseptic was developed using olanexidine gluconate in this study, and its virucidal efficacy against human noroviruses was evaluated using modified RT-qPCR that can account for genome derived from intact viruses using RNase A and photo-reactive intercalators. We tested the virucidal efficacy of five materials; two olanexidine gluconate antiseptics (hand rub formulation and surgical field formulation), two kinds of ethanol solutions at different pH (approx. 3 or 7), and a base component of olanexidine gluconate hand rub formulation against 11 human norovirus genotypes by culture-independent methods. The infectivity of murine norovirus (MNV), a surrogate for human norovirus, was significantly reduced after use of the antiseptics. The olanexidine gluconate hand rub demonstrated the strongest virucidal efficacy against human norovirus among the five tested materials. This study showed that olanexidine gluconate has the potential to become a strong tool for the prevention of human norovirus infection.
Improvement of Electrochemical Conditions for Detecting Redox Reaction of K₃[Fe(CN)₆] toward the Application in Norovirus Aptasensor Peer-reviewed

Seiya HIRANO, Junki SAITO, Tomoki YUKAWA, Daisuke SANO, Akihiro OKAMOTO, Satoshi OKABE, Masaaki KITAJIMA

Electrochemistry　88　(3)　205-209　2020/05/05
Publisher: The Electrochemical Society of Japan
DOI： 10.5796/electrochemistry.20-00017 　

ISSN： 1344-3542

eISSN： 2186-2451
Bottleneck size-eependent changes in the genetic diversity and specific growth rate of a rotavirus A strain International-journal Peer-reviewed

Syun-Suke Kadoya, Syun-Ichi Urayama, Takuro Nunoura, Miho Hirai, Yoshihiro Takaki, Masaaki Kitajima, Toyoko Nakagomi, Osamu Nakagomi, Satoshi Okabe, Osamu Nishimura, Daisuke Sano

Journal of Virology　94　(10)　e02083-19　2020/05/04

DOI： 10.1128/JVI.02083-19 　

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RNA viruses form a dynamic distribution of mutant swarms (termed "quasispecies") due to the accumulation of mutations in the viral genome. The genetic diversity of a viral population is affected by several factors, including a bottleneck effect. Human-to-human transmission exemplifies a bottleneck effect, in that only part of a viral population can reach the next susceptible hosts. In the present study, two lineages of the rhesus rotavirus (RRV) strain of rotavirus A were serially passaged five times at a multiplicity of infection (MOI) of 0.1 or 0.001, and three phenotypes (infectious titer, cell binding ability, and specific growth rate) were used to evaluate the impact of a bottleneck effect on the RRV population. The specific growth rate values of lineages passaged under the stronger bottleneck (MOI of 0.001) were higher after five passages. The nucleotide diversity also increased, which indicated that the mutant swarms of the lineages under the stronger bottleneck effect were expanded through the serial passages. The random distribution of synonymous and nonsynonymous substitutions on rotavirus genome segments indicated that almost all mutations were selectively neutral. Simple simulations revealed that the presence of minor mutants could influence the specific growth rate of a population in a mutant frequency-dependent manner. These results indicate a stronger bottleneck effect can create more sequence spaces for minor sequences.IMPORTANCE In this study, we investigated a bottleneck effect on an RRV population that may drastically affect the viral population structure. RRV populations were serially passaged under two levels of a bottleneck effect, which exemplified human-to-human transmission. As a result, the genetic diversity and specific growth rate of RRV populations increased under the stronger bottleneck effect, which implied that a bottleneck created a new space in a population for minor mutants originally existing in a hidden layer, which includes minor mutations that cannot be distinguished from a sequencing error. The results of this study suggest that the genetic drift caused by a bottleneck in human-to-human transmission explains the random appearance of new genetic lineages causing viral outbreaks, which can be expected according to molecular epidemiology using next-generation sequencing in which the viral genetic diversity within a viral population is investigated.
Assessment of microbial risks by characterization of Escherichia coli presence to analyze the public health risks from poor water quality in Nepal International-journal Peer-reviewed

Sital Uprety, Bipin Dangol, Pramina Nakarmi, Isha Dhakal, Samendra P Sherchan, Joanna L Shisler, Antarpreet Jutla, Mohan Amarasiri, Daisuke Sano, Thanh H Nguyen

International Journal of Hygiene and Environmental Health　226　113484-113484　2020/05

DOI： 10.1016/j.ijheh.2020.113484 　

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The combination of natural disasters and inadequate infrastructure introduce unique challenges in providing safe drinking water in low-income countries. For example, Nepal faces several challenges in managing sporadic diarrheal outbreaks across the country, given its complex geographic terrain, and its susceptibility to extreme natural events like earthquakes, floods, and landslides. To assess the risks of diarrheal diseases caused by fecal contamination in several water sources in different geographical regions of Nepal, we conducted a two months cross-sectional study throughout 37 out of 75 districts in Nepal, including the ones affected by Nepal 2015 earthquake. Quantitative Microbial Risk Assessment (QMRA) was applied to estimate the human health risk based on Escherichia coli (E. coli) count for 2,822 water samples collected at source and households. Disease burden calculations suggested that Hilly and Terai (low-land) regions are at the highest risk with 0.27 and 0.16 DALYs per person per year (DALYpppy), respectively, whereas mountain region disease burden was 0.02 DALYpppy. The risk comparison among exposure pathways indicated that the water used in households, including drinking water and water for washing, posed higher risks than from source water, reservoir water or tap water, suggesting deteriorated sanitation conditions in households. These findings suggest that the combination of QMRA and spatial analysis can help to prioritize intervention activities after a major natural disaster.
Required chlorination doses to fulfill the credit value for disinfection of enteric viruses in water: A critical review International-journal Peer-reviewed

Andri Taruna Rachmadi, Masaaki Kitajima, Tsuyoshi Kato, Hiroyuki Kato, Satoshi Okabe, Daisuke Sano

Environmental Science & Technology　54　(4)　2068-2077　2020/02/18

DOI： 10.1021/acs.est.9b01685 　

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A credit value of virus inactivation has been assigned to the disinfection step in international and domestic guidelines for wastewater reclamation and reuse. To fulfill the credit value for water disinfection, water engineers need to apply an appropriate disinfection strength, expressed as a CT value (mg × min/L), which is a product of disinfectant concentration and contact time, against enteric viruses in wastewater. In the present study, we extracted published experimental data on enteric virus inactivation using free chlorine and monochloramine and applied the Tobit analysis and simple linear regression analysis to calculate the range of CT values (mg × min/L) needed for 4-log10 inactivation. Data were selected from peer-reviewed papers containing kinetics data of virus infectivity and chlorine residual in water. Coxsackie B virus and echovirus require higher CT values (lower susceptibility) for 4-log10 inactivation than adenovirus and a human norovirus surrogate (murine norovirus) with free chlorine. On the other hand, adenovirus has lower susceptibility to monochloramine compared to murine norovirus, coxsackievirus, and echovirus. The factors that influence the required CT value are virus type, pH, water temperature, and water matrix. This systematic review demonstrates that enteroviruses and adenovirus are appropriate representative enteric viruses to evaluate water disinfection using free chlorine and monochloramine, respectively.
Regularized regression analysis for the prediction of virus inactivation efficiency by chloramine disinfection Peer-reviewed

Syun-suke Kadoya, Osamu Nishimura, Hiroyuki Kato, Daisuke Sano

Environmental Science: Water Research & Technology　6　(12)　3341-3350　2020
Publisher: Royal Society of Chemistry (RSC)
DOI： 10.1039/d0ew00539h 　

ISSN： 2053-1400

eISSN： 2053-1419
Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered Peer-reviewed

Mohan Amarasiri, Daisuke Sano, Satoru Suzuki

Critical Reviews in Environmental Science and Technology　50　(19)　2016-2059　2020
Publisher: Informa {UK} Limited
DOI： 10.1080/10643389.2019.1692611 　
Revisiting the effects of powdered activated carbon on membrane fouling mitigation in an anaerobic membrane bioreactor by evaluating long-term impacts on the surface layer International-journal Peer-reviewed

Zhen Lei, Shuming Yang, Xiang Li, Wen Wen, Xingyuan Huang, Yuan Yang, Xiaochang Wang, Yu-You Li, Daisuke Sano, Rong Chen

Water Research　167　115137-115137　2019/12/15

DOI： 10.1016/j.watres.2019.115137 　

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Two submerged anaerobic membrane bioreactors (AnMBRs) with and without powdered activated carbon (PAC) were studied to revisit the effect of PAC on membrane fouling performance by long-term operation when treating synthetic sewage. The results showed that PAC remained efficient for membrane fouling control after long-term operation (over 140 d), and it reduced the fouling rate at a hydraulic retention time of 8 h from 3.12 to 0.89 kPa/d. PAC mainly mitigated the membrane fouling by restraining the formation of a cake layer while generating a gel layer on the membrane surface, which was attributed to the PAC-induced microbial community change in mixed liquor and the membrane surface. Microbial community analysis indicated the genera Pseudomonas (26.5%) and Methanothrix (79.21%) were the predominant bacteria and archaea, respectively, in the gel layer, and this result is completely different from the presence of a high abundance of Levilinea (7.1%), Aminivibrio (4.9%) and Methanothrix (90.04%) in the cake layer on the membrane surface without PAC. The significant difference in the predominant microbes in the membrane surface layer was attributed to the reduced enrichment of Levilinea and Methanothrix with PAC addition.
Health Risk Management for the Users of a household-installed water supply system Based on Water Safety Planning Approach Peer-reviewed

門屋俊祐, 牛島健, 伊藤竜生, 長谷川祥樹, 三浦尚之, 秋葉道宏, 西村修, 佐野大輔

土木学会論文集　75　(7)　III_403-III_412　2019/12
Predictive water virology: Hierarchical Bayesian modeling for estimating virus inactivation curve Peer-reviewed

Syun-suke Kadoya, Osamu Nishimura, Hiroyuki Kato, Daisuke Sano

Water　11　(10)　2187　2019/10

DOI： 10.3390/w11102187 　

eISSN： 2073-4441
Fecal Source Tracking in A Wastewater Treatment and Reclamation System Using Multiple Waterborne Gastroenteritis Viruses. International-journal Peer-reviewed

Zheng Ji, Xiaochang C Wang, Limei Xu, Chongmiao Zhang, Cheng Rong, Andri Taruna Rachmadi, Mohan Amarasiri, Satoshi Okabe, Naoyuki Funamizu, Daisuke Sano

Pathogens (Basel, Switzerland)　8　(4)　170-170　2019/09/30
Publisher: {MDPI} {AG}
DOI： 10.3390/pathogens8040170 　

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Gastroenteritis viruses in wastewater reclamation systems can pose a major threat to public health. In this study, multiple gastroenteritis viruses were detected from wastewater to estimate the viral contamination sources in a wastewater treatment and reclamation system installed in a suburb of Xi'an city, China. Reverse transcription plus nested or semi-nested PCR, followed by sequencing and phylogenetic analysis, were used for detection and genotyping of noroviruses and rotaviruses. As a result, 91.7% (22/24) of raw sewage samples, 70.8% (17/24) of the wastewater samples treated by anaerobic/anoxic/oxic (A2O) process and 62.5% (15/24) of lake water samples were positive for at least one of target gastroenteritis viruses while all samples collected from membrane bioreactor effluent after free chlorine disinfection were negative. Sequence analyses of the PCR products revealed that epidemiologically minor strains of norovirus GI (GI/14) and GII (GII/13) were frequently detected in the system. Considering virus concentration in the disinfected MBR effluent which is used as the source of lake water is below the detection limit, these results indicate that artificial lake may be contaminated from sources other than the wastewater reclamation system, which may include aerosols, and there is a possible norovirus infection risk by exposure through reclaimed water usage and by onshore winds transporting aerosols containing norovirus.
Sign-constrained linear regression for prediction of microbe concentration based on water quality datasets International-journal Peer-reviewed

Tsuyoshi Kato, Ayano Kobayashi, Wakana Oishi, Syun-suke Kadoya, Satoshi Okabe, Naoya Ohta, Mohan Amarasiri, Daisuke Sano

Journal of Water and Health　17　(3)　404-415　2019/06

DOI： 10.2166/wh.2019.001 　

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This study presents a novel methodology for estimating the concentration of environmental pollutants in water, such as pathogens, based on environmental parameters. The scientific uniqueness of this study is the prevention of excess conformity in the model fitting by applying domain knowledge, which is the accumulated scientific knowledge regarding the correlations between response and explanatory variables. Sign constraints were used to express domain knowledge, and the effect of the sign constraints on the prediction performance using censored datasets was investigated. As a result, we confirmed that sign constraints made prediction more accurate compared to conventional sign-free approaches. The most remarkable technical contribution of this study is the finding that the sign constraints can be incorporated in the estimation of the correlation coefficient in Tobit analysis. We developed effective and numerically stable algorithms for fitting a model to datasets under the sign constraints. This novel algorithm is applicable to a wide variety of the prediction of pollutant contamination level, including the pathogen concentrations in water.
Specific interactions between human norovirus and environmental matrices: Effects on the virus ecology Peer-reviewed

Mohan Amarasiri, Daisuke Sano

Viruses　11　(3)　224　2019/03
Specific interactions of rotavirus HAL1166 with Enterobacter cloacae SENG-6 and their contribution on rotavirus HAL1166 removal Peer-reviewed

Mohan Amarasiri, Hiroki Kawai, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano

Water Science and Technology　79　(2)　342-348　2019/01
Publisher: {IWA} Publishing
DOI： 10.2166/wst.2019.050 　

ISSN： 0273-1223
Assays for specific growth rate and cell-binding ability of rotavirus Peer-reviewed

Syun-suke Kadoya, Daisuke Sano

Journal of Visualized Experiments　143　e58821　2019/01
Free-Chlorine Disinfection as a Selection Pressure on Norovirus. International-journal Peer-reviewed

Andri Taruna Rachmadi, Masaaki Kitajima, Kozo Watanabe, Sakiko Yaegashi, Joeselle Serrana, Arata Nakamura, Toyoko Nakagomi, Osamu Nakagomi, Kazuhiko Katayama, Satoshi Okabe, Daisuke Sano

Applied and environmental microbiology　84　(13)　2018/07/01

DOI： 10.1128/AEM.00244-18 　

ISSN： 0099-2240
Virus Particle Detection by Convolutional Neural Network in Transmission Electron Microscopy Images Peer-reviewed

Eisuke Ito, Takaaki Sato, Daisuke Sano, Etsuko Utagawa, Tsuyoshi Kato

Food and Environmental Virology　10　(2)　201-208　2018/06/01

DOI： 10.1007/s12560-018-9335-7 　

ISSN： 1867-0342 1867-0334
A review on recent progress in the detection methods and prevalence of human enteric viruses in water Peer-reviewed

Eiji Haramoto, Masaaki Kitajima, Akihiko Hata, Jason R. Torrey, Yoshifumi Masago, Daisuke Sano, Hiroyuki Katayama

Water Research　135　168-186　2018/05/15

DOI： 10.1016/j.watres.2018.02.004 　

ISSN： 1879-2448 0043-1354

eISSN： 1879-2448
Microfluidic PCR amplification and MiSeq amplicon sequencing techniques for high-throughput detection and genotyping of human pathogenic RNA viruses in human feces, sewage, and oysters Peer-reviewed

Mamoru Oshiki, Takayuki Miura, Shinobu Kazama, Takahiro Segawa, Satoshi Ishii, Masashi Hatamoto, Takashi Yamaguchi, Kengo Kubota, Akinori Iguchi, Tadashi Tagawa, Tsutomu Okubo, Shigeki Uemura, Hideki Harada, Naohiro Kobayashi, Nobuo Araki, Daisuke Sano

Frontiers in Microbiology　9　830　2018/04/27

DOI： 10.3389/fmicb.2018.00830 　

ISSN： 1664-302X
Reverse transcription-quantitative PCR assays for genotype-specific detection of human noroviruses in clinical and environmental samples Peer-reviewed

Mohan Amarasiri, Masaaki Kitajima, Akiho Miyamura, Ricardo Santos, Silvia Monteiro, Takayuki Miura, Shinobu Kazama, Satoshi Okabe, Daisuke Sano

International Journal of Hygiene and Environmental Health　221　(3)　578-585　2018/04

DOI： 10.1016/j.ijheh.2018.02.008 　

ISSN： 1438-4639
Selection of cellular genetic markers for the detection of infectious poliovirus Peer-reviewed

D. Sano, M. Tazawa, M. Inaba, S. Kadoya, R. Watanabe, T. Miura, M. Kitajima, S. Okabe

Journal of Applied Microbiology　124　(4)　1001-1007　2018/04/01
Publisher: Blackwell Publishing Ltd
DOI： 10.1111/jam.13621 　

ISSN： 1365-2672 1364-5072
Disinfection as a Selection Pressure on RNA Virus Evolution. International-journal

Andri Taruna Rachmadi, Masaaki Kitajima, Kozo Watanabe, Satoshi Okabe, Daisuke Sano

Environmental science & technology　52　(5)　2434-2435　2018/03/06

DOI： 10.1021/acs.est.8b00590 　

ISSN： 0013-936X
Sapovirus in wastewater treatment plants in Tunisia: Prevalence, removal, and genetic characterization Peer-reviewed

Miguel F. Varela, Imen Ouardani, Tsuyoshi Kato, Syunsuke Kadoya, Mahjoub Aouni, Daisuke Sano, Jesús L. Romalde

Applied and Environmental Microbiology　84　(6)　e02093-17　2018/03/01

DOI： 10.1128/AEM.02093-17 　

ISSN： 1098-5336 0099-2240
Identification of the inactivating factors and mechanisms exerted on MS2 coliphage in concentrated synthetic urine Peer-reviewed

Wakana Oishi, Daisuke Sano, Loic Decrey, Syunsuke Kadoya, Tamar Kohn, Naoyuki Funamizu

Science of the Total Environment　598　213-219　2017/11

DOI： 10.1016/j.scitotenv.2017.04.088 　

ISSN： 0048-9697

eISSN： 1879-1026
Target virus log(10) reduction values determined for two reclaimed wastewater irrigation scenarios in Japan based on tolerable annual disease burden Peer-reviewed

Toshihiro Ito, Masaaki Kitajima, Tsuyoshi Kato, Satoshi Ishii, Takahiro Segawa, Satoshi Okabe, Daisuke Sano

Water Research　125　438-448　2017/11

DOI： 10.1016/j.watres.2017.08.057 　

ISSN： 0043-1354
Bacteriophage removal efficiency as a validation and operational monitoring tool for virus reduction in wastewater reclamation: Review Peer-reviewed

Mohan Amarasiri, Masaaki Kitajima, Thanh H. Nguyen, Satoshi Okabe, Daisuke Sano

Water Research　121　258-269　2017/09

DOI： 10.1016/j.watres.2017.05.035 　

ISSN： 0043-1354
下水再生処理におけるヒト腸管系ウイルスの目標除去効率の算定法 Peer-reviewed

伊藤寿宏, 押木守, 小林直央, 加藤毅, 瀬川高弘, 幡本将史, 山口隆司, 原田秀樹, 北島正章, 岡部聡, 佐野大輔

環境工学研究論文集　72　(7)　305-313　2017/04/03
Publisher: 土木学会
DOI： 10.2208/jscejer.72.III_305 　

ISSN： 2185-6648
Removal of human pathogenic viruses in a down-flow hanging sponge (DHS) reactor treating municipal wastewater and health risks associated with utilization of the effluent for agricultural irrigation Peer-reviewed

Naohiro Kobayashi, Mamoru Oshiki, Toshihiro Ito, Takahiro Segawa, Masashi Hatamoto, Tsuyoshi Kato, Takashi Yamaguchi, Kengo Kubota, Masanobu Takahashi, Akinori Iguchi, Tadashi Tagawa, Tsutomu Okubo, Shigeki Uemura, Hideki Harada, Toshiki Motoyama, Nobuo Araki, Daisuke Sano

Water Research　110　389-398　2017/03

DOI： 10.1016/j.watres.2016.10.054 　

ISSN： 0043-1354
Evaluation of virus reduction efficiency in wastewater treatment unit processes as a credit value in the multiple-barrier system for wastewater reclamation and reuse Peer-reviewed

Toshihiro Ito, Tsuyoshi Kato, Makoto Hasegawa, Hiroyuki Katayama, Satoshi Ishii, Satoshi Okabe, Daisuke Sano

Journal of Water and Health　14　(6)　879-889　2016/12

DOI： 10.2166/wh.2016.096 　

ISSN： 1477-8920
Bactericidal and virucidal mechanisms in the alkaline disinfection of compost using calcium lime and ash Peer-reviewed

Nowaki Hijikata, Rui Tezuka, Shinobu Kazama, Masahiro Otaki, Ken Ushijima, Ryusei Ito, Satoshi Okabe, Daisuke Sano, Naoyuki Funamizu

Journal of Environmental Management　181　721-727　2016/10

DOI： 10.1016/j.jenvman.2016.08.026 　

ISSN： 0301-4797

eISSN： 1095-8630
Effect of leaf surface chemical properties on efficacy of sanitizer for rotavirus inactivation Peer-reviewed

Miyu Fuzawa, Kang-Mo Ku, Sindy Paola Palma-Salgado, Kenya Nagasaka, Hao Feng, John A. Juvik, Daisuke Sano, Joanna L. Shisler, Thanh H. Nguyen

Applied and Environmental Microbiology　82　(20)　6214-6222　2016/10

DOI： 10.1128/AEM.01778-16 　

ISSN： 0099-2240

eISSN： 1098-5336
Risk management of viral infectious diseases in wastewater reclamation and reuse: Review Peer-reviewed

Daisuke Sano, Mohan Amarasiri, Akihiko Hata, Toru Watanabe, Hiroyuki Katayama

Environment International　91　220-229　2016/05

DOI： 10.1016/j.envint.2016.03.001 　

ISSN： 0160-4120
Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane Peer-reviewed

Mohan Amarasiri, Satoshi Hashiba, Takayuki Miura, Toyoko Nakagomi, Osamu Nakagomi, Satoshi Ishii, Satoshi Okabe, Daisuke Sano

Water Research　95　383-391　2016/05

DOI： 10.1016/j.watres.2016.04.018 　

ISSN： 0043-1354
EVIDENCE-BASED DETERMINATION OF THE HYGIENE STANDARD VALUE IN ENVIRONMENTAL WATER Peer-reviewed

KOBAYASHI Ayano, SANO Daisuke, KATO Tsuyoshi, ITO Toshihiro, MIYAMURA Akiho, MIURA Takayuki, ISHII Satoshi, OKABE Satoshi

Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research)　72　(3)　40-49　2016/03
Publisher: Japan Society of Civil Engineers
DOI： 10.2208/jscejer.72.40 　

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 The present study proposes an approach for obtaining a candidate value of a water hygiene standard using water quality monitoring datasets and acceptable infection risks of waterborne pathogenic microbes. In the proposed approach, posterior predictive distributions of the concentration of an indicator microorganism and a pathogen-derived gene are estimated separately based on monitoring datasets, and then the probabilistic distribution of concentration ratio between the indicator microorganism and the pathogen-derived gene is obtained. In addition to it, an acceptable concentration of a pathogen-derived gene in water is derived from an acceptable infection risk (10^-4 infections per person per year) by quantitative microbial risk assessment (QMRA). The candidate value of water hygiene standard is determined by collating the calculated acceptable concentration of a pathogen-derived gene and the estimated distribution of concentration ratio. When a safety factor value is set at 10, 150 most probable number/ 100mL of Escherichia coli was obtained as a candidate value of water hygiene standard for river water that is used as a drinking water source. Although this study identified research areas that have to be investigated further, such as the validation of QMRA assumptions, the proposed approach makes it possible to determine water hygiene standard values based on the scientific basis, including accumulated datasets of environmental water monitoring.
Estimation of concentration ratio of indicator to pathogen-related gene in environmental water based on left-censored data Peer-reviewed

Tsuyoshi Kato, Ayano Kobayashi, Toshihiro Ito, Takayuki Miura, Satoshi Ishii, Satoshi Okabe, Daisuke Sano

Journal of Water and Health　14　(1)　14-25　2016/02

DOI： 10.2166/wh.2015.029 　

ISSN： 1477-8920
Environment and gastroenteritis viruses: Roles of virus-binding bacteria

SANO Daisuke

Uirusu　66　(2)　179-186　2016
Publisher: The Japanese Society for Virology
DOI： 10.2222/jsv.66.179 　

ISSN： 0042-6857

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Gastroenteritis virises, including human norovirus and rotavirus, are transmitted not only through humans but also via contaminated water, foods and environmental fomites such as door knob. It is thus important to disinfect these contaminated stuffs for controlling infectious diseases caused by gastroenteritis viruses. The author of this article has been investigating histo-blood group antigen (HBGA)-positive bacteria as a possible environmental vehicle of human norovirus and rotavirus. In this article, recent publications related to the effect of HBGA-positive bacteria on the life cycles of gastroenteritsi viruses are introduced.
Genome sequence of Enterobacter cloacae strain SENG-6, a bacterium producing histo-blood group antigen-like substances that can bind with human noroviruses

Ishii, S., Amarasiri, M., Hashiba, S., Yang, P., Okabe, S., Sano, D.

Genome Announcements　4　(4)　e00893-16　2016
Publisher: American Society for Microbiology
DOI： 10.1128/genomeA.00893-16 　
Bayesian modeling of virus removal efficiency in wastewater treatment processes Peer-reviewed

T. Ito, T. Kato, K. Takagishi, S. Okabe, D. Sano

Water Science and Technology　72　(10)　1789-1795　2015/11

DOI： 10.2166/wst.2015.402 　

ISSN： 0273-1223

eISSN： 1996-9732
Removal properties of human enteric viruses in a pilot-scale membrane bioreactor (MBR) process Peer-reviewed

Takayuki Miura, Satoshi Okabe, Yoshihito Nakahara, Daisuke Sano

Water Research　75　282-291　2015/05

DOI： 10.1016/j.watres.2015.02.046 　

ISSN： 0043-1354
Culture-independent evaluation of nonenveloped-virus infectivity reduced by free-chlorine disinfection Peer-reviewed

Daisuke Sano, Takatomo Ohta, Arata Nakamura, Toyoko Nakagomi, Osamu Nakagomi, Satoshi Okabe

Applied and Environmental Microbiology　81　(8)　2819-2826　2015/04

DOI： 10.1128/AEM.03802-14 　

ISSN： 0099-2240

eISSN： 1098-5336
Molecular epidemiology of noroviruses detected in Nepalese children with acute diarrhea between 2005 and 2011: Increase and predominance of minor genotype GII.13 Peer-reviewed

Thi Nguyen Hoa-Tran, Toyoko Nakagomi, Daisuke Sano, Jeevan B. Sherchand, Basu D. Pandey, Nigel A. Cunliffe, Osamu Nakagomi

Infection Genetics and Evolution　30　27-36　2015/03

DOI： 10.1016/j.meegid.2014.12.003 　

ISSN： 1567-1348

eISSN： 1567-7257
マイクロバイオームとウイロームマイクロバイオーム衛生環境工学分野におけるマイクロバイオーム研究 Invited

井原賢, 稲葉愛美, 佐野大輔

臨床と微生物　42　(6)　685-689　2015

ISSN： 0910-7029
将来の社会システムにおける感染性胃腸炎リスク Invited

佐野大輔

最新医学　70　2415-2422　2015
水中の健康関連微生物に関する研究動向

片山浩之, 安井宣仁, 猪又明子, 田中宏明, 岸田直裕, 原本英司, 渡部徹, 真砂佳史, 浅田安廣, 端昭彦, 佐野大輔, 井原賢, 稲葉愛美

水環境学会誌　37(A)　(12)　424-433　2014/12/10
Publisher:
ISSN： 0916-8958
Microfluidic quantitative PCR for simultaneous quantification of multiple viruses in environmental water samples Peer-reviewed

Satoshi Ishii, Gaku Kitamura, Takahiro Segawa, Ayano Kobayashi, Takayuki Miura, Daisuke Sano, Satoshi Okabe

Applied and Environmental Microbiology　80　(24)　7505-7511　2014/12

DOI： 10.1128/AEM.02578-14 　

ISSN： 0099-2240

eISSN： 1098-5336
External CO2 and water supplies for enhancing electrical power generation of air-cathode microbial fuel cells Peer-reviewed

So Ishizaki, Itto Fujiki, Daisuke Sano, Satoshi Okabe

Environmental Science & Technology　48　(19)　11204-11210　2014/10

DOI： 10.1021/es5021197 　

ISSN： 0013-936X

eISSN： 1520-5851
Estimation of contamination sources of human enteroviruses in a wastewater treatment and reclamation system by PCR-DGGE Peer-reviewed

Zheng Ji, Xiaochang C. Wang, Limei Xu, Chongmiao Zhang, Naoyuki Funamizu, Satoshi Okabe, Daisuke Sano

Food and Environmental Virology　6　(2)　99-109　2014/06

DOI： 10.1007/s12560-014-9140-x 　

ISSN： 1867-0334

eISSN： 1867-0342
Water quality monitoring and risk assessment by simultaneous multipathogen quantification Peer-reviewed

Satoshi Ishii, Takamitsu Nakamura, Shuji Ozawa, Ayano Kobayashi, Daisuke Sano, Satoshi Okabe

Environmental Science & Technology　48　(9)　4744-4749　2014/05

DOI： 10.1021/es500578s 　

ISSN： 0013-936X

eISSN： 1520-5851
水環境中からのウイルス情報の収集とその活用・総説 Invited

佐野大輔

臨床とウイルス　42　(5)　211-223　2014
培養できないウイルスの感染性の評価法 Invited

佐野大輔, 岡部聡

感染と消毒　21　(2)　24-27　2014
Bayesian modeling of enteric virus density in wastewater using left-censored data Peer-reviewed

Tsuyoshi Kato, Takayuki Miura, Satoshi Okabe, Daisuke Sano

Food and Environmental Virology　5　(4)　185-193　2013/12

DOI： 10.1007/s12560-013-9125-1 　

ISSN： 1867-0334

eISSN： 1867-0342
Use of a genetically-engineered Escherichia coli strain as a sample process control for quantification of the host-specific bacterial genetic markers Peer-reviewed

Ayano Kobayashi, Daisuke Sano, Asami Taniuchi, Satoshi Ishii, Satoshi Okabe

Applied and Environmental Microbiology　97　(20)　9165-9173　2013/10

DOI： 10.1007/s00253-013-5188-z 　

ISSN： 0175-7598
Histo-blood group antigen-like substances of human enteric bacteria as specific adsorbents for human noroviruses Peer-reviewed

Takayuki Miura, Daisuke Sano, Atsushi Suenaga, Takeshi Yoshimura, Miyu Fuzawa, Toyoko Nakagomi, Osamu Nakagomi, Satoshi Okabe

Journal of Virology　87　(17)　9441-9451　2013/09

DOI： 10.1128/JVI.01060-13 　

ISSN： 0022-538X
Chicken- and duck-associated Bacteroides-Prevotella genetic markers for detecting fecal contamination in environmental water Peer-reviewed

Ayano Kobayashi, Daisuke Sano, Jun Hatori, Satoshi Ishii, Satoshi Okabe

Applied Microbiology and Biotechnology　97　(16)　7427-7437　2013/08

DOI： 10.1007/s00253-012-4469-2 　

ISSN： 0175-7598
水環境中の病原ウイルス分析手法

三浦尚之, 佐野大輔

日本水環境学会誌　5　151-155　2013
生活用水に潜むウイルス対策 Invited

佐野大輔

バムサジャーナル　25　(4)　8-13　2013
A new approach for evaluating the infectivity of noncultivatable enteric viruses without cell culture Peer-reviewed

Kazuki Tojo, Daisuke Sano, Takayuki Miura, Toyoko Nakagomi, Osamu Nakagomi, Satoshi Okabe

Water Science and Technology　67　(10)　2236-2240　2013

DOI： 10.2166/wst.2013.114 　

ISSN： 0273-1223

eISSN： 1996-9732
Effects of temperature and predator on the persistence of host-specific Bacteroides-Prevotella genetic markers in water Peer-reviewed

Ayano Kobayashi, Daisuke Sano, Satoshi Okabe

Water Science and Technology　67　(4)　838-845　2013

DOI： 10.2166/wst.2012.626 　

ISSN： 0273-1223
Occurrence of Hand-Foot-and-Mouth Disease Pathogens in Domestic Sewage and Secondary Effluent in Xi'an, China Peer-reviewed

Zheng Ji, Xiaochang Wang, Chongmiao Zhang, Takayuki Miura, Daisuke Sano, Naoyuki Funamizu, Satoshi Okabe

MICROBES AND ENVIRONMENTS　27　(3)　288-292　2012/09

DOI： 10.1264/jsme2.ME11352 　

ISSN： 1342-6311
Human norovirus occurrence and diversity in the Llobregat river catchment, Spain Peer-reviewed

Unai Perez-Sautu, Daisuke Sano, Susana Guix, Georg Kasimir, Rosa M. Pinto, Albert Bosch

ENVIRONMENTAL MICROBIOLOGY　14　(2)　494-502　2012/02

DOI： 10.1111/j.1462-2920.2011.02642.x 　

ISSN： 1462-2912
Adsorption characteristics of an enteric virus-binding protein to norovirus, rotavirus and poliovirus Peer-reviewed

Takahiro Imai, Daisuke Sano, Takayuki Miura, Satoshi Okabe, Keishi Wada, Yoshifumi Masago, Tatsuo Omura

BMC Biotechnology　11　2011/12/16

DOI： 10.1186/1472-6750-11-123 　

ISSN： 1472-6750
Development of an Effective Method for Recovery of Viral Genomic RNA from Environmental Silty Sediments for Quantitative Molecular Detection Peer-reviewed

Takayuki Miura, Yoshifumi Masago, Daisuke Sano, Tatsuo Omura

APPLIED AND ENVIRONMENTAL MICROBIOLOGY　77　(12)　3975-3981　2011/06

DOI： 10.1128/AEM.02692-10 　

ISSN： 0099-2240
Development and validation of a microarray for the confirmation and typing of norovirus RT-PCR products Peer-reviewed

Kirsten Mattison, Nathalie Corneau, Ingvild Berg, Albert Bosch, Erwin Duizer, Ion Gutierrez-Aguirre, Yvan L'Homme, Yalda Lucero, Zhiyao Luo, Alexander Martyres, Mette Myrmel, Miguel O'Ryan, Franco Pagotto, Daisuke Sano, Sanela Svraka, Ulises Urzua, Sabah Bidawid

JOURNAL OF VIROLOGICAL METHODS　173　(2)　233-250　2011/05

DOI： 10.1016/j.jviromet.2011.02.011 　

ISSN： 0166-0934
感染性胃腸炎の診断,治療,伝播予防 7.感染性胃腸炎と上下水道の衛生工学的管理

佐野大輔

化学療法の領域　27　(4)　734-740　2011/03

ISSN： 0913-2384
Analytical Methods for Virus Detection in Water and Food Peer-reviewed

Albert Bosch, Gloria Sanchez, Morteza Abbaszadegan, Annalaura Carducci, Susana Guix, Francoise S. Le Guyader, Rembuluwani Netshikweta, Rosa M. Pinto, Wim H. M. van der Poel, Saskia Rutjes, Daisuke Sano, Maureen B. Taylor, Walda B. van Zyl, David Rodriguez-Lazaro, Katarina Kovac, Jane Sellwood

FOOD ANALYTICAL METHODS　4　(1)　4-12　2011/03

DOI： 10.1007/s12161-010-9161-5 　

ISSN： 1936-9751

eISSN： 1936-976X
Quantification and Genotyping of Human Sapoviruses in the Llobregat River Catchment, Spain Peer-reviewed

Daisuke Sano, Unai Perez-Sautu, Susana Guix, Rosa Maria Pinto, Takayuki Miura, Satoshi Okabe, Albert Bosch

APPLIED AND ENVIRONMENTAL MICROBIOLOGY　77　(3)　1111-1114　2011/02

DOI： 10.1128/AEM.01721-10 　

ISSN： 0099-2240
Identification and characterization of coagulation inhibitor proteins derived from cyanobacterium Microcystis aeruginosa. Peer-reviewed

Sano, Daisuke, Ishifuji, Shingo, Sato, Yuichi, Imae, Yasutaka, Takaara, Tomoko, Masago, Yoshifumi, Omura, Tatsuo

Chemosphere　82　(8)　1096-1102　2011

DOI： 10.1016/j.chemosphere.2010.12.005 　

ISSN： 0045-6535
Norovirus-binding proteins recovered from activated sludge micro-organisms with an affinity to a noroviral capsid peptide Peer-reviewed

D. Sano, K. Wada, T. Imai, Y. Masago, T. Omura

JOURNAL OF APPLIED MICROBIOLOGY　109　(6)　1923-1928　2010/12

DOI： 10.1111/j.1365-2672.2010.04821.x 　

ISSN： 1364-5072
ノロウイルス制御―院内伝播防止に向けて 2)水系管理によるノロウイルス感染症の制御の可能性

佐野大輔

感染対策ICTジャーナル　5　(4)　421-426　2010/10

ISSN： 1881-4964
Detection of Sapovirus in oysters Peer-reviewed

You Ueki, Mika Shoji, Yoko Okimura, Yasuko Miyota, Yoshifumi Masago, Tomoichiro Oka, Kazuhiko Katayama, Naokazu Takeda, Mamoru Noda, Takayuki Miura, Daisuke Sano, Tatsuo Omura

MICROBIOLOGY AND IMMUNOLOGY　54　(8)　483-486　2010/08

DOI： 10.1111/j.1348-0421.2010.00239.x 　

ISSN： 0385-5600
Surface-retained organic matter of Microcystis aeruginosa inhibiting coagulation with polyaluminum chloride in drinking water treatment Peer-reviewed

Tomoko Takaara, Daisuke Sano, Yoshifumi Masago, Tatsuo Omura

WATER RESEARCH　44　(13)　3781-3786　2010/07

DOI： 10.1016/j.watres.2010.04.030 　

ISSN： 0043-1354
Microcystis aeruginosa由来凝集阻害誘因タンパク質の同定 Peer-reviewed

石藤慎吾, 佐藤有一, 今江泰貴, 高荒智子, 佐野大輔, 真砂佳史, 大村達夫

水環境学会誌　33　(6)　73-79　2010/06
Publisher: Japan Society on Water Environment
DOI： 10.2965/jswe.33.73 　

ISSN： 0916-8958

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Excess growth of cyanobacteria in semiclosed water area caused by eutrophication induces coagulation inhibition in drinking water treatment processes. Thus, it is highly desirable to establish effective countermeasures against such coagulation inhibition by identifying the causative substances released by cyanobacteria. In this study, coagulation inhibitor proteins of Microcystis aeruginosa were screened using a phage display system and an aluminum-ion-immobilized affinity chromatography. From the results, algal proteins with a high level of metal-coordinating amino acids were identified as inhibitor proteins for the coagulation. One of the inhibitor proteins, a homologue of the metal sensor protein ArsR, showed strong inhibition of the coagulation of kaolin suspension with polyaluminum chloride (PAC). It was demonstrated that one of the mechanisms of the coagulation inhibition caused by M. aeruginosa is the inhibition of the cross-linking between aluminum clusters in PAC and suspended solids by the algal inhibitor protein.
Detection of Oxidative Damages on Viral Capsid Protein for Evaluating Structural Integrity and Infectivity of Human Norovirus Peer-reviewed

Daisuke Sano, Rosa M. Pinto, Tatsuo Omura, Albert Bosch

ENVIRONMENTAL SCIENCE & TECHNOLOGY　44　(2)　808-812　2010/01

DOI： 10.1021/es9018964 　

ISSN： 0013-936X
Genetic Diversity and Amino Acid Substitutions among Norovirus Genogroup II Strains in Environmental and Stool Samples Peer-reviewed

IMAI TAKAHIRO, SANO DAISUKE, MASAGO YOSHIFUMI, UEKI YO, FUKUSHI KENSUKE, OMURA TATSUO

環境工学研究論文集　45　(45)　355-360　2008/11
Publisher: Japan Society of Civil Engineers
DOI： 10.11532/proes1992.45.355 　

ISSN： 1341-5115

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Capsid gene sequences of norovirus GII were acquired from patients of gastroenteritis, primary-treated wastewater, activated sludge, secondary-treated wastewater, river water, seawater and oyster in geographically close areas between 2001 and 2006. Most of the norovirus GII genes acquired from gastroenteritis patients were mainly categorized into GII.3 and GII.4. On the other hand, diverse genotypes of norovirus GII including GII.2, 3, 4, 5, 10, 11, 14and 15 were obtained from environmental samples. Amino acid sequences of norovirus GII.4 obtained in this study were compared with that of norovirus GII.4 Bristol. As a result, amino acid substitutions were observed in the 8th (Ala), 9th (Asn), 15th (Ala) and 54th (Asn) residues. The analysis of amino acid substitutions and investigation of the appearance of norovirus mutants could be useful to understand the dynamics of norovirus in human society.
Enzymatic Virus Elution (EVE) Method for Elution of Enteric Viruses in Digestive Diverticulum of Japanese Oyster, Crassostrea gigas Peer-reviewed

OKUMURA CHIE, MASAGO YOSHIFUMI, SANO DAISUKE, UEKI YO, OMURA TATSUO

環境工学研究論文集　45　179-186　2008/11
Publisher: Japan Society of Civil Engineers
DOI： 10.11532/proes1992.45.179 　

ISSN： 1341-5115

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A new virus elution method from digestive diverticulum of Japanese oyster, Crassostrea gigas, using enzymatic virus elution (EVE) method was developed using poliovirus 1 as a surrogate. Among three enzymes, amylase, lipase and pepsin, lipase and amylase were effective for elution of viruses from digestive diverticulum collected in winter, with total recovery efficiency of 11% and 9.6%, respectively. Lipase was also effective for virus elution from digestive diverticula collected in spring when oysters are in reproductive phase and contain more fat in their digestive diverticula. In conclusion, the EVE method using lipase is very effective in quantitative evaluation of virus accumulation by oysters and its seasonal trends.
New tools for the study and direct surveillance of viral pathogens in water Peer-reviewed

Albert Bosch, Susana Guix, Daisuke Sano, Rosa M. Pinto

CURRENT OPINION IN BIOTECHNOLOGY　19　(3)　295-301　2008/06

DOI： 10.1016/j.copbio.2008.04.006 　

ISSN： 0958-1669
活性汚泥細菌から分離されたノロウイルス吸着タンパク質 (Norovirus-Binding Proteins: NoVBPs) の特性評価 Peer-reviewed

和田圭史, 佐野大輔, 今井崇博, 大村達夫

水環境学会誌　30　(12)　731-736　2007/12
Publisher: Japan Society on Water Environment
DOI： 10.2965/jswe.30.731 　

ISSN： 0916-8958

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In this study, norovirus-binding proteins (NoVBPs) were newly recovered from activated sludge culture using affinity chromatography. The C-terminal amino acid sequence (12 residues) of the Lordsdale virus, belonging to the dominant genotype (GII.4) of noroviruses throughout the world, was used as a ligand in the affinity chromatography. Two-dimensional electrophoresis analysis shows that the samples recovered by the affinity chromatography include a variety of proteins, which have a molecular weights between 60 and 10 kDa and isoelectric points between 3 and 6. The binding affinity of NoVBPs to the virus-like particle (VLP) of norovirus GII.4 was higher than that to the VLP of norovirus GI.1, which was indicated by ELISA. A determination of the N-terminal amino acid sequences revealed that several NoVBPs are relatives to a chaperonine protein, GroEL, which can interact with denatured hydrophobic proteins. The NoVBPs recovered in this study might be useful as versatile adsorbents for norovirus recovery or detection from environmental water samples.
Persistence of caliciviruses in artificially contaminated oysters during depuration Peer-reviewed

You Ueki, Mika Shoji, Atsushi Suto, Toru Tanabe, Yoko Okimura, Yoshihiko Kikuchi, Noriyuki Saito, Daisuke Sano, Tatsuo Omura

APPLIED AND ENVIRONMENTAL MICROBIOLOGY　73　(17)　5698-5701　2007/09

DOI： 10.1128/AEM.00290-07 　

ISSN： 0099-2240
Human sapovirus in clams, Japan Peer-reviewed

Grant S. Hansman, Tomoichiro Oka, Reiko Okamoto, Tomoko Nishida, Shoichi Toda, Mamoru Noda, Daisuke Sano, You Ueki, Takahiro Imai, Tatsuo Omura, Osamu Nishio, Hirokazu Kimura, Naokazu Takeda

EMERGING INFECTIOUS DISEASES　13　(4)　620-622　2007/04

DOI： 10.3201/eid1304.061390 　

ISSN： 1080-6040
Cellular proteins of Microcystis aeruginosa inhibiting coagulation with polyaluminum chloride Peer-reviewed

Tomoko Takaara, Daisuke Sano, Hiroshi Konno, Tatsuo Omura

Water Research　41　1653-1658.　2007

DOI： 10.1016/j.watres.2007.01.035 　
Sapovirus in water, Japan Peer-reviewed

Grant S. Hansman, Daisuke Sano, You Ueki, Takahiro Imai, Tomoichiro Oka, Kazuhiko Katayama, Naokazu Takeda, Tatsuo Omura

Emerging Infectious Diseases　13　(1)　133-135　2007/01

DOI： 10.3201/eid1301.061047 　

ISSN： 1080-6040

eISSN： 1080-6059
Genetic variation in the conservative gene region of norovirus genogroup II strains in environmental and stool samples Peer-reviewed

Daisuke Sano, You Ueki, Toru Watanabe, Tatsuo Omura

ENVIRONMENTAL SCIENCE & TECHNOLOGY　40　(23)　7423-7427　2006/12

DOI： 10.1021/es0603453 　

ISSN： 0013-936X
凝集阻害を引き起こすMicrocystis aeruginosa由来親水性物質の分離及び特性評価 Peer-reviewed

高荒智子, 佐野大輔, 須藤丈, 今野弘, 大村達夫

環境工学研究論文集　43　585-591　2006/11
Publisher: Japan Society of Civil Engineers
DOI： 10.11532/proes1992.43.585 　

ISSN： 1341-5115

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Mass propagation of algae in drinking water source affects the coagulation process in water treatment systems. Algogenic organic matter (AOM) interacts with coagulants or suspended substances, and brings about several disorders in water treatment processes, but the inhibitory mechanism on the coagulation by AOMhas remained to be elucidated. In this study, hydrophilic substances extracted from Microcystis aeruginosa (M aeruginosa) were found to involve potent inhibitory substances for coagulation with polyaluminum chloride. Those inhibitory substances were possible to be precipitated by ethanol. The fractionation of the precipitated AOMs with ultrafiltration and anion exchange enable us to confirm that the inhibitory substances have a net negative charge under the pH value of 8.0, and the molecular weights of those are larger than 10kDa. Lipopolysaccharide and RNA could be the possible inhibitory substances involved in the hydrophilic substances from M. aeruginosa, and how this algal organic matter induces the coagulation inhibition would be elucidated in the further study.
Acquisition of Algal Genes Coding Proteins Responsible for Coagulation Inhibition Using Phage Display Technique Peer-reviewed

SANO DAISUKE, TAKAARA TOMOKO, SUZUKI TAKAYOSHI, OMURA TATSUO

環境工学研究論文集　43　127-133　2006/11
Publisher: Japan Society of Civil Engineers
DOI： 10.11532/proes1992.43.127 　

ISSN： 1341-5115

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Seasonal overgrowth of cyanobacteria such as Microcystis aeruginosa (M. aeruginosa) is one of serious problems in semi-closed water area including reservoirs. It is known that algogenic organic matters bring about the inhibition of coagulation on the flocculation process in drinking water treatment systems. However, the inhibitory organic matters produced by algae have remained to be elucidated. In this study, phage display library of proteins from M. aeruginosa was used to isolate algal proteins responsible for the coagulation inhibition. Several algal genes coding possible inhibitory proteins were obtained by biopanning using polyaluminum chloride. Amino acid sequences of proteins deduced from the obtained gene suggested that hydroxyl groups in serine and threonine, formamide groups in glutamine and aspargine, thiol groups in cysteine and carboxyl groups in glutamic acid and aspartic acid were involved in the coagulation inhibition. The result of the homology search suggested that metallloenzymes such as glycosyl transferase could be also responsible for the coagulation inhibition.
Cloning of a heavy-metal-binding protein derived from activated-sludge microorganisms Peer-reviewed

Daisuke Sano, Ken Myojo, Tatsuo Omura

APPLIED AND ENVIRONMENTAL MICROBIOLOGY　72　(9)　6377-6380　2006/09

DOI： 10.1128/AEM.00656-06 　

ISSN： 0099-2240
Contamination of Sea Area with Human Pathogenic Viruses

SANO Daisuke, UEKI You, OMURA Tatsuo

Bulletin of the Society of Sea Water Science, Japan　60　(4)　229-237　2006
Publisher: The Society of Sea Water Science, Japan
DOI： 10.11457/swsj1965.60.229 　

ISSN： 0369-4550

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Human pathogenic viruses such as Noroviruses and Rotaviruses contaminate water environments, including estuary and coastal areas. These pathogenic viruses in sea area would pose risks for infectious diseases among humans who eat contaminated sea products or play in contaminated bathing areas. It is of primary importance to comprehend the actual situation of the virus contamination of sea areas, which could be regarded as one of considerable reservoirs of human pathogenic viruses. In this article, recent knowledge in regards to the fate of human pathogenic viruses in sea areas is reviewed, and the moving pathway of these viruses estimated by scientific evidences is discussed.
河川及び海域における病原ウイルス汚染-ノロウイルス調査事例

佐野大輔, 植木洋

日本水環境学会誌　29　(3)　130-134　2006
水中病原ウイルスによる水環境汚染の実態

佐野大輔, 植木洋, 渡部徹

モダンメディア　52　(2)　115-124　2006
Membrane separation of indigenous noroviruses from sewage sludge and treated wastewater Peer-reviewed

D. Sano, Y. Ueki, T. Watanabe, T. Omura

WATER SCIENCE AND TECHNOLOGY　54　(3)　77-82　2006

DOI： 10.2166/wst.2006.451 　

ISSN： 0273-1223
Heavy metal-binding proteins from metal-stimulated bacteria as a novel adsorbent for metal removal technology Peer-reviewed

D Sano, K Myojo, T Omura

WATER SCIENCE AND TECHNOLOGY　53　(6)　221-226　2006

DOI： 10.2166/wst.2006.200 　

ISSN： 0273-1223
活性汚泥細菌ゲノムDNAライブラリから取得した新規重金属吸着タンパク質遺伝子 Peer-reviewed

佐野大輔, 明星賢, 大村達夫

環境工学研究論文集　42　601-608　2005/12
Publisher: Japan Society of Civil Engineers
DOI： 10.11532/proes1992.42.601 　

ISSN： 1341-5115

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Gene of heavy metal-binding protein (HIVIBP) was newly isolated from genomic DNA library of activated sludge bacteria. In order to obtain the objective gene on the strength of N-terminal amino acid sequence of HMBP, a set of gene screening method was constructed. The constructed screening method employs CODEHOP method, touchdown PCR and semi-nested PCR, which makes it possible to amplify the specific HMBP gene. Two acidic amino acids (aspartic acid and glutamic acid) occupied 24% of a deduced amino acid sequence of HMBP, and the rate of metal-coordinating amino acids (aspartic acid, glutamic acid, serine, methionine and histidine) among the deduced sequence of HMBP reached 35%. The estimated secondary and tertiary structures revealed that HMIBP is composed of abundance of alpha helices in which amino acid side chains expose to outside. A lot of metal-coordinating residues projecting out from the alpha helices would confer the heavy metal-binding ability on HMBP.
Norovirus pathway in water environment estimated by genetic analysis of strains from patients of gastroenteritis, sewage, treated wastewater, river water and oysters Peer-reviewed

Y Ueki, D Sano, T Watanabe, K Akiyama, T Omura

WATER RESEARCH　39　(18)　4271-4280　2005/11

DOI： 10.1016/j.watres.2005.06.035 　

ISSN： 0043-1354
Construction of a cloning system for the mass production of a virus-binding protein specific for poliovirus type 1 Peer-reviewed

D Sano, T Omura

APPLIED AND ENVIRONMENTAL MICROBIOLOGY　71　(5)　2608-2615　2005/05

DOI： 10.1128/AEM.71.5.2608-2615.2005 　

ISSN： 0099-2240
Affinity Isolation of Algal Organic Matters Able to Form Complex with Aluminum Coagulant Peer-reviewed

Tomoko Takaara, Daisuke Sano, Hiroshi Konno, Tatsuo Omura

Water Science and Technology: Water Supply　4　(5-6)　95-102　2005
アデノウイルス吸着タンパク質(Adenovirus‐Binding Protein:ADVBP)の活性汚泥細菌からの分離 Peer-reviewed

佐野大輔, 石井哲平, 大村達夫

環境工学研究論文集　41　331-337　2004/11
Publisher:
DOI： 10.11532/proes1992.41.331 　

ISSN： 1341-5115
Virus-binding proteins recovered from bacterial culture derived from activated sludge by affinity chromatography assay using a viral capsid peptide Peer-reviewed

D Sano, T Matsuo, T Omura

APPLIED AND ENVIRONMENTAL MICROBIOLOGY　70　(6)　3434-3442　2004/06

DOI： 10.1128/AEM.70.6.3434-3442.2004 　

ISSN： 0099-2240
凝集阻害を誘因する藻類由来タンパク質の同定を目的としたファージディスプレイの構築 Peer-reviewed

鈴木孝佳, 高荒智子, 佐野大輔, 大村達夫

環境工学研究論文集　41　339-346　2004
Publisher:
DOI： 10.11532/proes1992.41.339 　

ISSN： 1341-5115
Detection of infectious pathogenic viruses in water and wastewater samples from urbanised areas Peer-reviewed

D Sano, T Watanabe, T Matsuo, T Omura

WATER SCIENCE AND TECHNOLOGY　50　(1)　247-251　2004

ISSN： 0273-1223
Isolation of alminium-complexing organic matters originated from algae Peer-reviewed

TAKAARA TOMOKO, MARUYAMA AKIKO, SANO DAISUKE, KONNO HIROSHI, OMURA TATSUO

環境工学研究論文集　40　227-236　2003/11
Publisher: Japan Society of Civil Engineers
DOI： 10.11532/proes1992.40.227 　

ISSN： 1341-5115

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Mass propagation of algae in raw water has been known related to disturbing the flocculation in drinking watertreatmentsystems. Many researchers proposed that algal organic matters (AOMs) can interact with flocculants by coordinate bonds, and bring about several disorders due to increases in flocculant demand and in the residual flocculant in treated water. However, the inhibitory mechanism by AOMs have not been elucidated In this study, alminium-complexing substances in AOMs were successfully isolated with affinity chromatography. SDS-PAGE analysis revealed that these substances include proteins which have molecular weight between 40 and 70kDa. However, the results of the gel chromatography show that the amount of these proteins in AOMs were estimated to be very low compared to organic matters with high molecular weights around 2000kDa. These results imply that not only proteins but also other organic matters such as polysaccharides contribute to the inhibition of flocculation.
Detection of enteric viruses in municipal sewage sludge by a combination of the enzymatic virus elution method and RT-PCR Peer-reviewed

D Sano, K Fukushi, Y Yoshida, T Omura

WATER RESEARCH　37　(14)　3490-3498　2003/08

DOI： 10.1013/S0043-1354(03)00208-2 　

ISSN： 0043-1354
Functional metal-binding proteins by metal-stimulated bacteria for the development of an innovative metal removal technology Peer-reviewed

T Antsuki, D Sano, T Omura

WATER SCIENCE AND TECHNOLOGY　47　(10)　109-115　2003

ISSN： 0273-1223
アフィニティクロマトグラフィによる活性汚泥からのウイルス吸着タンパク質（Virus-Binding Protein：VBP）の分離と特性評価 Peer-reviewed

松尾崇宏, 佐野大輔, 大村達夫

環境工学研究論文集　39　345-353　2002
Publisher: Japan Society of Civil Engineers
DOI： 10.11532/proes1992.39.345 　

ISSN： 1341-5115

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Water pollution with pathogenic viruses has been frequently reported in recent years. Since conventionalwater and wastewater treament systems are not enough to inactivate or remove pathogenic viruses, a new technology for virus removal should be developed. In this study, virus-binding proteins (VBPs) were isolated from activated sludge culture with affinity chomatography. The ability of VBPs to capture intact particles of poliovirus I was confirmed with ELISA. SDS-PAGE analysis revealed that VBPs included a number of polypeptidesthat have molecular weight between 94.0 and 14.4k Da. The evaluation of surface charges of VBPs with ion exchange chromatography found that a majority of VBP molecules had a net negative charge under the condition of the affinity chromatography. On the other hand, the viral peptide, which was used as an affinity ligand, was expected to have a net negative charge. As a result, the VBPs must be captured by the viral peptide with stronger binding forces than the electrostatic repulsive force. The affinity adsorption could explain the strong attractive interaction between VBPs and viral peptide. These VBPs could be useful as an innovative material for the virus removal.
Risk evaluation for pathogenic bacteria and viruses in sewages sludge compost Peer-reviewed

T. Watanabe, D. Sano, T. Omura

Water Science and Technology　46　(11-12)　325-330　2002

ISSN： 0273-1223
Enhanced virus recovery front municipal sewage sludge with a combination of enzyme and cation exchange resin Peer-reviewed

D Sano, K Fukushi, K Yano, Y Yoshida, T Omura

WATER SCIENCE AND TECHNOLOGY　43　(2)　75-82　2001

ISSN： 0273-1223
Survey of copper-binding proteins in the activated sludge culture. Peer-reviewed

ANTSUKI TARO, SANO DAISUKE, FUKUI KENSUKE, OMURA TATSUO

環境工学研究論文集　37　131-140　2000/11
Publisher: Japan Society of Civil Engineers
DOI： 10.11532/proes1992.37.131 　

ISSN： 1341-5115

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The toxic metal pollution is one of the major problems in environmental waters. Although the metal pollution has not been recently reported because of the development of successful removal technologies, the problem at relatively low concentration of metals still remains. The enhanced recovery of metals from water and wastewater becomes the social needs because metals have the toxicity to humans even at such low concentrations. Metals in wastewater adsorb easily to proteins in activated sludge and metals adsorbed are immediately dissociated with the increase of hydrogen ion concentration. These properties are available for both a metal recovery from environmental waters and a recycling after the metal recovery.<BR>In this study, proteins that had the high affinity to copper (copper-binding proteins) were isolated from activated sludge culture using the affinity chromatography. As the result of the SDS-PAGE, the proteins were divided into two groups by their molecular weights. One group was from 43kDa to 30kDa and another was less than 30kDa. The latter group was the copper-binding proteins produced when copper ion was present as an inducer in the activated sludge culture.

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Misc. 77

Sensitivity Evaluation of Enveloped and Non-enveloped Viruses to Ethanol Using Machine Learning: A Systematic Review

Aken Puti Wanguyun, Wakana Oishi, Daisuke Sano

Food and Environmental Virology　16　(1)　1-13　2024/03

DOI： 10.1007/s12560-023-09571-2 　

ISSN： 1867-0334

eISSN： 1867-0342
Resource recovery technologies as microbial risk barriers: towards safe use of excreta in agriculture based on hazard analysis and critical control point

Wakana Oishi, Björn Vinnerås, Daisuke Sano

Environmental Science: Water Research and Technology　9　(4)　1008-1029　2023/03/01

DOI： 10.1039/d2ew00832g 　

ISSN： 2053-1400

eISSN： 2053-1419
Development of a Prediction Model for algal Bloom Occurrence in Water Sources Using Satellite Data

三浦耀平, 今本博臣, 峠嘉哉, 浅田安廣, 下ケ橋雅樹, 秋葉道宏, 西村修, 佐野大輔

日本水環境学会年会講演集　57th　2023
PREDICTING MARINE PRIMARY PRODUCTION BY MACHINE LEARNING

丸尾知佳子, 坂巻隆史, 佐野大輔, 西村修

土木学会論文集(Web)　79　(25)　2023

ISSN： 2436-6021
Trends in Research and Activities Related to Health-Related Water Microbiology

片山浩之, 原本英司, 佐野大輔, 渡部徹, 西山正晃, 井原賢, 端昭彦, 五味良太, 鳥居将太郎, 安井碧, 大石若菜

水環境学会誌　46(A)　(12)　2023

ISSN： 0916-8958
A national survey on the occurrence of pepper mild mottle virus as a genetic marker of virus removal in water purification processes

三浦尚之, 瀧野博之, 門屋俊祐, 三浦耀平, 佐野大輔, 秋葉道宏

日本水環境学会年会講演集　56th　2022
気候変動に伴う水道システムの生物障害等リスク評価とその適応性の強化に向けた研究ダム湖の藻類異常発生予測モデルの構築における衛星データ等の活用可能性の検討

西村修, 佐野大輔, 今本博臣, 三浦耀平

気候変動に伴う水道システムの生物障害等リスク評価とその適応性の強化に向けた研究令和3年度総括・分担研究報告書(Web)　2022
Construction of Electrochemical Sensor for Detecting Infection Disease-Related Biomarkers in Wastewater

山崎綾乃, 北島正章, 佐野大輔

日本水環境学会年会講演集　56th　2022
微生物燃料電池(MFC : Microbial Fuel Cells)による二酸化炭素ガス回収・固定技術

宇田川洋一, 高塚威, 佐野大輔, 石﨑創

30　(9)　29-32　2021/09

ISSN： 0918-7510
Detection of SARS-CoV-2 in Wastewater from a COVID-19 Quarantine Hotel: Demonstration of the Applicability of Wastewater-Based Epidemiology to Facility-Level Surveillance

北島正章, 岩本遼, 佐野大輔, 原本英司, 山口貴世志, 空野すみれ, 空野すみれ, 山岸拓也, 村上道夫, 小林博幸, 井元清哉, 岡部聡

日本水環境学会年会講演集　55th　2021
Relationship between Viral Infections, Routes of Transmission to Humans and Infection Control : To Prevent Airborne Transmission

空気調和・衛生工学会論文集　(286)　55-58　2021/01
Publisher: 空気調和・衛生工学会
ISSN： 0385-275X
Relationship between Viral Infections, Routes of Transmission to Humans and Infection Control: To Prevent Airborne Transmission

高塚威, 宇田川洋一, 北島正章, 佐野大輔, 宇田川悦子

空気調和・衛生工学会論文集　(286)　2021

ISSN： 0385-275X
微生物燃料電池(MFC:Microbial Fuel Cells)による二酸化炭素ガス回収・固定技術

宇田川洋一, 高塚威, 佐野大輔, 石崎創

クリーンエネルギー　30　(9)　2021

ISSN： 0918-7510
Carbon Dioxide Gas Capture and Fixation Technology using Microbial Fuel Cells

宇田川洋一, 高塚威, 佐野大輔, 石崎創

日本機械学会年次大会講演論文集(CD-ROM)　2021　2021

ISSN： 2424-2667
微生物燃料電池(MFC;Microbial Fuel Cells)による二酸化炭素ガス回収・固定技術

宇田川洋一, 高塚威, 佐野大輔, 石崎創

新日本空調技術開発研究所技報　(26)　2021
Intra Population Genetic Diversity of Rotavirus A Strain Impacts on Free Chlorine Sensitivity

門屋俊祐, 西村修, 佐野大輔, 浦山俊一, 布浦拓郎, 北島正章, 岡部聡, 中込とよ子, 中込治

日本水環境学会年会講演集　54th　2020
Intra-Population Diversity of Rotavirus Changes the Chlorine Sensitivity

門屋俊祐, 浦山俊一, 布浦拓郎, 中込治, 中込とよ子, 北島正章, 岡部聡, 西村修, 佐野大輔

日本水環境学会シンポジウム講演集　23rd　2020
ウイルス感染症とヒトへの感染と空気-空気を介した感染を防ぐために-

高塚威, 宇田川洋一, 北島正章, 佐野大輔, 宇田川悦子, 宇田川悦子

新日本空調技術開発研究所技報　(25)　2020
共存細菌との結合が水系感染症ウイルスの感染効率に与える影響

河合大樹, 北島正章, 岡部聡, 佐野大輔

日本水環境学会年会講演集　52nd　2018
DNAアプタマーを用いた簡易ノロウイルス検出法の開発

吉原光, 北島正章, 岡部聡, 高橋正宏, 佐藤久, 佐野大輔

日本水環境学会年会講演集　52nd　2018
環境水から単離した溶菌性バクテリオファージによる水中病原細菌の低減効果

高木達馬, 佐野大輔, 岡部聡, 北島正章

日本水環境学会年会講演集　52nd　2018
ウイルス干渉現象がIntegrated cell culture-PCRによる感染性ウイルス検出に与える影響

渡邊亮介, 北島正章, 岡部聡, 佐野大輔

土木学会北海道支部論文報告集(CD-ROM)　(74)　2018
ウイルス干渉現象がIntegrated cell culture-PCRによる感染性アデノウイルス検出に与える影響

佐野大輔, 渡邊亮介, 北島正章, 岡部聡

日本水環境学会シンポジウム講演集　21st　2018
ナノ流路デバイスを用いた下水中RNAウイルス定量およびタイピング:DHSリアクターへの適用例

押木守, 小林直央, 荒木信夫, 伊藤寿宏, 佐野大輔, 三浦尚之, 風間しのぶ, 原田秀樹, 久保田健吾, 幡本将史, 山口隆司, 井口晃徳, 瀬川高弘, 加藤毅, 多川正, 大久保努, 上村繁樹, 高橋優信

日本水環境学会年会講演集　51st　546　2017/03/09
遊離塩素耐性ロタウイルスの変異遺伝子同定に関する研究

門屋俊祐, 浦山俊一, 布浦拓郎, 北島正章, 岡部聡, 佐野大輔

日本水環境学会年会講演集　51st　2017
DNAアプタセンサー:水中ノロウイルスの即時・高感度検出に適した新技術

平野誠也, 岡本章玄, 佐野大輔, 岡部聡, 北島正章

日本水環境学会年会講演集　51st　2017
病原性レジオネラの水系感染制御に資する溶菌性バクテリオファージの探索

高木達馬, 佐野大輔, 岡部聡, 北島正章

日本水環境学会年会講演集　51st　2017
水環境におけるノロウイルスの遺伝子型別存在実態の把握

宮村明帆, 植木洋, 三浦尚之, 風間しのぶ, 北島正章, 岡部聡, 佐野大輔

日本水環境学会年会講演集　51st　2017
ウイルス吸着性細菌との結合が水系感染症ウイルスの感染能力に与える影響

河合大樹, 中込とよ子, 中込治, 北島正章, 岡部聡, 佐野大輔

日本水環境学会年会講演集　51st　2017
環境水中からのノロウイルス吸着性大腸菌の単離と吸着因子同定に関する研究

羽柴聡, AMARASIRI Mohan, 原本英司, 北島正章, 岡部聡, 佐野大輔

日本水環境学会年会講演集　51st　2017
遊離塩素耐性ロタウイルスの変異遺伝子および耐性メカニズムの解明

門屋俊祐, 浦山俊一, 布浦拓郎, 北島正章, 岡部聡, 佐野大輔

環境工学研究フォーラム講演集　54th　2017
水系感染性病原細菌に対するバクテリオファージの単離および溶菌特性解析

高木達馬, 佐野大輔, 岡部聡, 北島正章

環境工学研究フォーラム講演集　54th　2017
DNAアプタマーを用いた簡易ノロウイルス検出法の開発

吉原光, 北島正章, 佐野大輔, 岡部聡, 高橋正宏, 佐藤久

環境工学研究フォーラム講演集　54th　2017
下水再生利用のためのノロウイルス目標除去効率算定に関する研究

伊藤寿宏, 北島正章, 加藤毅, 石井聡, 瀬川高弘, 岡部聡, 佐野大輔

日本微生物生態学会大会(Web)　2017　2017
組織細胞におけるイオンチャネル遺伝子発現プロファイルに着目した水中感染性エンテロウイルス迅速検出手法の開発

渡邊亮介, 稲葉愛美, 岡部聡, 佐野大輔, 北島正章

日本水環境学会年会講演集　51st　2017
DNAアプタマーを用いた簡易ノロウイルス検出法の開発

吉原光, 北島正章, 佐野大輔, 岡部聡, 高橋正宏, 佐藤久

全国会議(水道研究発表会)講演集　2017　2017

ISSN： 2436-1496
Aptamer: The potential application to norovirus research, diagnosis, and therapeutics

Seiya Hirano, Daisuke Sano, Satoshi Okabe, Masaaki Kitajima

Noroviruses: Outbreaks, Control and Prevention Strategies　223-242　2017/01/01
DHS(downflow hanging sponge)リアクターにおける病原ウイルスの除去効率

小林直央, 押木守, 荒木信夫, 本山毅宜, 伊藤寿宏, 佐野大輔, 瀬川高弘, 幡本将史, 山口隆司, 井口晃徳, 上村繁樹, 大久保努, 多川正, 高橋優信, 久保田健吾, 原田秀樹

日本水環境学会年会講演集　50th　657　2016/03/10
遊離塩素処理がノロウイルスの遺伝的多様性に与える影響

中村新, 渡辺幸三, 八重樫咲子, 岡部聡, 中込とよ子, 中込治, 佐野大輔

日本水環境学会年会講演集　50th　398　2016/03/10
ヒト細胞由来遺伝子マーカーを用いた感染性ウイルスの迅速検出

稲葉愛美, 伊藤寿宏, 大村達夫, 岡部聡, 佐野大輔

日本水環境学会年会講演集　50th　2016
ノロウイルスの新知見

門屋俊祐, 伊藤寿宏, 佐野大輔

膜協会ジャーナル　23　(1)　7-15　2016
ノロウイルスの塩素消毒耐性獲得メカニズム解明に関する基礎的研究

富岡哲史, 渡辺幸三, 岡部聡, 佐野大輔

土木学会北海道支部論文報告集(CD-ROM)　(70)　ROMBUNNO.G-01　2014/02
表面プラズモン共鳴を利用した病原微生物バイオセンサの開発

坂槙有紀恵, 山田健太, 佐野大輔, 高橋正宏, 岡部聡, 佐藤久

日本水環境学会年会講演集　47th　436　2013/03/11
表面プラズモン共鳴を利用した病原微生物バイオセンサの開発

坂槙有紀恵, 山田健太, ピタックティーラタムニティ, 石井聡, 佐野大輔, 高橋正宏, 岡部聡, 佐藤久

環境工学研究フォーラム講演集　49th　28-30　2012/11/28
表面プラズモン共鳴を利用した水中病原微生物検出バイオセンサの開発

佐藤久, 坂槙有紀恵, 山田健太, ピタックティーラタムニティ, 石井聡, 佐野大輔, 高橋正宏, 岡部聡

日本水環境学会シンポジウム講演集　15th　233-234　2012/09/10
活性汚泥からのウイルス吸着タンパク質分離方法の新規開発

今井崇博, 佐野大輔, 真砂佳史, 大村達夫

日本水環境学会年会講演集　45th　441　2011/03/18
Norovirus-binding proteins recovered from activated sludge micro-organisms with an affinity to a noroviral capsid peptide

D. Sano, K. Wada, T. Imai, Y. Masago, T. Omura

JOURNAL OF APPLIED MICROBIOLOGY　109　(6)　1923-1928　2010/12

DOI： 10.1111/j.1365-2672.2010.04821.x 　

ISSN： 1364-5072
Detection of Sapovirus in oysters

You Ueki, Mika Shoji, Yoko Okimura, Yasuko Miyota, Yoshifumi Masago, Tomoichiro Oka, Kazuhiko Katayama, Naokazu Takeda, Mamoru Noda, Takayuki Miura, Daisuke Sano, Tatsuo Omura

MICROBIOLOGY AND IMMUNOLOGY　54　(8)　483-486　2010/08

DOI： 10.1111/j.1348-0421.2010.00239.x 　

ISSN： 0385-5600
Surface-retained organic matter of Microcystis aeruginosa inhibiting coagulation with polyaluminum chloride in drinking water treatment

Tomoko Takaara, Daisuke Sano, Yoshifumi Masago, Tatsuo Omura

WATER RESEARCH　44　(13)　3781-3786　2010/07

DOI： 10.1016/j.watres.2010.04.030 　

ISSN： 0043-1354
環境水中におけるノロウイルス(NoV)の挙動

植木洋, 庄司美加, 上村弘, 沖村容子, 御代田恭子, 今井崇博, 三浦尚之, 真砂佳史, 大村達夫, 佐野大輔, 西尾治

日本水環境学会シンポジウム講演集　11th　182-183　2008/09/17
ガラスビーズへのウイルス吸着タンパク質(Virus‐Binding Prote in:VBP)固定化手法の確立

今井崇博, 真砂佳史, 佐野大輔, 大村達夫

土木学会年次学術講演会講演概要集(CD-ROM)　63rd　(Disk 2)　ROMBUNNO.7-160　2008/08/13
New tools for the study and direct surveillance of viral pathogens in water

Albert Bosch, Susana Guix, Daisuke Sano, Rosa M. Pinto

CURRENT OPINION IN BIOTECHNOLOGY　19　(3)　295-301　2008/06

DOI： 10.1016/j.copbio.2008.04.006 　

ISSN： 0958-1669
Enzymatic Virus Elution(EVE)法による牡蠣中腸腺からのウイルス誘出技術の検討

今井崇博, 奥村千恵, 佐野大輔, 真砂佳史, 大村達夫

日本水環境学会年会講演集　42nd　460　2008/03/19
Invenstigation of virus removal with membrance separation activated sludge process

OMURA tatsuo, WATANABE Toru, SANO Daisuke, UEKI You

Annual report of the Society for the Promotion of Construction Engineering　42　1-6　2007/04/01
Publisher: Tohoku University
ISSN： 0288-9994
Cellular proteins of Microcystis aeruginosa inhibiting coagulation with polyaluminum chloride

Tomoko Takaara, Daisuke Sano, Hiroshi Konno, Tatsuo Omura

WATER RESEARCH　41　(8)　1653-1658　2007/04

DOI： 10.1016/j.watres.2007.01.035 　

ISSN： 0043-1354
Human sapovirus in clams, Japan

Grant S. Hansman, Tomoichiro Oka, Reiko Okamoto, Tomoko Nishida, Shoichi Toda, Mamoru Noda, Daisuke Sano, You Ueki, Takahiro Imai, Tatsuo Omura, Osamu Nishio, Hirokazu Kimura, Naokazu Takeda

EMERGING INFECTIOUS DISEASES　13　(4)　620-622　2007/04

DOI： 10.3201/eid1304.061390 　

ISSN： 1080-6040
Virus-binding in water environments: natural ligands for human viruses

D. Sano, T. Omura

Environmental Microbiology Research Trends　257-272　2007
Sapovirus in water, Japan

Grant S. Hansman, Daisuke Sano, You Ueki, Takahiro Imai, Tomoichiro Oka, Kazuhiko Katayama, Naokazu Takeda, Tatsuo Omura

EMERGING INFECTIOUS DISEASES　13　(1)　133-135　2007/01

DOI： 10.3201/eid1301.061047 　

ISSN： 1080-6040
Genetic variation in the conservative gene region of norovirus genogroup II strains in environmental and stool samples

Daisuke Sano, You Ueki, Toru Watanabe, Tatsuo Omura

ENVIRONMENTAL SCIENCE & TECHNOLOGY　40　(23)　7423-7427　2006/12

DOI： 10.1021/es0603453 　

ISSN： 0013-936X
Cloning of a heavy-metal-binding protein derived from activated-sludge microorganisms

Daisuke Sano, Ken Myojo, Tatsuo Omura

APPLIED AND ENVIRONMENTAL MICROBIOLOGY　72　(9)　6377-6380　2006/09

DOI： 10.1128/AEM.00656-06 　

ISSN： 0099-2240
Investigation of Norovirus removal from treated wastewater

MURA Tatsuo, WATANABE Toru, SANO Daisuke, UEKI You

Annual report of the Society for the Promotion of Construction Engineering　41　28-32　2006/04/01
Publisher: Tohoku University
ISSN： 0288-9994
Heavy metal-binding proteins from metal-stimulated bacteria as a novel adsorbent for metal removal technology

D Sano, K Myojo, T Omura

WATER SCIENCE AND TECHNOLOGY　53　(6)　221-226　2006

DOI： 10.2166/wst.2006.200 　

ISSN： 0273-1223
Membrane separation of indigenous noroviruses from sewage sludge and treated wastewater

D. Sano, Y. Ueki, T. Watanabe, T. Omura

WATER SCIENCE AND TECHNOLOGY　54　(3)　77-82　2006

DOI： 10.2166/wst.2006.451 　

ISSN： 0273-1223
Norovirus pathway in water environment estimated by genetic analysis of strains from patients of gastroenteritis, sewage, treated wastewater, river water and oysters

Y Ueki, D Sano, T Watanabe, K Akiyama, T Omura

WATER RESEARCH　39　(18)　4271-4280　2005/11

DOI： 10.1016/j.watres.2005.06.035 　

ISSN： 0043-1354
Construction of a cloning system for the mass production of a virus-binding protein specific for poliovirus type 1

Daisuke Sano, Tatsuo Omura

Applied and Environmental Microbiology　71　(5)　2608-2615　2005/05

DOI： 10.1128/AEM.71.5.2608-2615.2005 　

ISSN： 0099-2240
Evaluation of Virus Removal Efficiency with Membrane Separation Activated Sludge

OMURA Tatsuo, WATANABE Toru, SANO Daisuke, UEKI You

Annual report of the Society for the Promotion of Construction Engineering　40　49-55　2005/04/01
Publisher: Tohoku University
ISSN： 0288-9994
Investigation of SRSV Removal from Treated Wastewater

OMURA Tatsuo, WATANABE Toru, SANO Daisuke, KUMAGAI Yukihiro

Annual report of the Society for the Promotion of Construction Engineering　40　42-48　2005/04/01
Publisher: Tohoku University
ISSN： 0288-9994
Virus-binding proteins recovered from bacterial culture derived from activated sludge by affinity chromatography assay using a viral capsid peptide

D Sano, T Matsuo, T Omura

APPLIED AND ENVIRONMENTAL MICROBIOLOGY　70　(6)　3434-3442　2004/06

DOI： 10.1128/AEM.70.6.3434-3442.2004 　

ISSN： 0099-2240
Investigation of Pathogenic Viruses in Wastewater

OMURA Tatsuo, WATANABE Toru, SANO Daisuke, KUMAGAI Yukihiro

Annual report of the Society for the Promotion of Construction Engineering　39　61-67　2004/04/01
Publisher: Tohoku University
ISSN： 0288-9994
Detection of infectious pathogenic viruses in water and wastewater samples from urbanised areas

D Sano, T Watanabe, T Matsuo, T Omura

WATER SCIENCE AND TECHNOLOGY　50　(1)　247-251　2004

ISSN： 0273-1223
Affinity isolation of algal organic matters able to form complex with aluminium coagulant

T Takaara, D Sano, H Konno, T Omura

4TH WORLD WATER CONGRESS: INNOVATION IN DRINKING WATER TREATMENT　4　(5-6)　95-102　2004

ISSN： 1606-9749
Detection of enteric viruses in municipal sewage sludge by a combination of the enzymatic virus elution method and RT-PCR

D Sano, K Fukushi, Y Yoshida, T Omura

WATER RESEARCH　37　(14)　3490-3498　2003/08

DOI： 10.1013/S0043-1354(03)00208-2 　

ISSN： 0043-1354
Functional metal-binding proteins by metal-stimulated bacteria for the development of an innovative metal removal technology

T Antsuki, D Sano, T Omura

WATER SCIENCE AND TECHNOLOGY　47　(10)　109-115　2003

ISSN： 0273-1223
Risk evaluation for pathogenic bacteria and viruses in sewage sludge compost

T Watanabe, D Sano, T Omura

WATER SCIENCE AND TECHNOLOGY　46　(11-12)　325-330　2002

ISSN： 0273-1223
Enhanced virus recovery front municipal sewage sludge with a combination of enzyme and cation exchange resin

D Sano, K Fukushi, K Yano, Y Yoshida, T Omura

WATER SCIENCE AND TECHNOLOGY　43　(2)　75-82　2001

ISSN： 0273-1223
Enhanced Extraction of Enteric Virus from Sewage Sludge in the Presense of Emzymes with Chelater.

佐野大輔, 福士謙介, 大村達夫

日本水環境学会年会講演集　33rd　1999
Effectiveness of polysaccharide degrading enzyme in virus elicitation from sludge.

佐野大輔, 福士謙介, 熊谷幸博, 大村達夫

土木学会年次学術講演会講演概要集第7部　53rd　1998

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Books and Other Publications 3

環境と微生物の事典

2014
水再生利用学

浅野孝, 大垣眞一郎, 江藤隆, 滝沢智, 船水尚行, 松井正樹, 田中宏明

技法堂出版　2010
Virus-binding proteins in water environments: natural ligands for human viruses, p. 257-272. In George V. Kurladze (ed.), Environmental Microbiology Research Trends.

Nova Science Publishers Inc., Hauppauge, N.Y.　2008

Presentations 48

Diverse enteric bacteria can excrete histo-blood group antigens as adsorbents for human gastroenteritis viruses International-presentation

Daisuke Sano

The 63rd Annual Meeting of the Japanese Society for Virology　2015/11
Estimation Accuracy of Pathogen Density Distribution in Water Based on a Left-Censored Dataset: Bayesian vs Maximum a Posteriori International-presentation

Daisuke Sano

IEEC2015　2015/10
衛生環境工学におけるゲノム解析技術の応用 Invited

佐野大輔

第158回日本獣医学会学術集会・公衆衛生学分科会シンポジウム「ゲノム解析技術が生み出す獣医公衆衛生学領域における新知見紹介」　2015/09/07
Diversity of histo-blood group antigen-positive enteric bacteria in water: possible environmental vehicle for human norovirus International-presentation

Daisuke Sano

WaterMicro2015　2015/09
下水再生利用に関わる下水処理装置の性能評価及び運転管理手法の提案 Invited

佐野大輔

7. 土木学会環境工学委員会・次世代下水道小委員会講演会　2015/08/11
胃腸炎ウイルスの特性と水系感染リスク制御 Invited

佐野大輔

京都大学大学院工学研究科・環境微生物学特論　2015/07/07
Genetic variations of norovirus GII.4 within a single host and among hosts International-presentation

Daisuke Sano

Special Workshop on Health-Related Environmental Virology　2015/05/27
胃腸炎ウイルスの感染制御因子 Invited

佐野大輔

第11回小児消化管感染症研究会　2015/02/07
胃腸炎ウイルスの環境中動態を含めた生態の解明に向けて

佐野大輔

5. 第11回小児消化管感染症研究会　2014/11/09
Bayesian modeling of virus removal efficiency in wastewater treatment processes International-presentation

Daisuke Sano

IWA World Water Congress & Exhibition　2014/09
Estimation of quantitative relationships between pathogens and indicator microorganisms in water using left-censored data International-presentation

Daisuke Sano

ISFEV2014　2014/09
Development of a screening assay of norovirus-binding enteric bacteria bearing histo-blood group antigen-like substances International-presentation

Daisuke Sano

IUMS2014　2014/07
Research trends and future perspectives of environmental and public health virology International-presentation

Daisuke Sano

The 4th Young Professors and Students Symposium　2014/03/27
Norovirus-binding enteric bacteria: Significance in the environmental dissemination of gastroenteritis viruses International-presentation

Daisuke Sano

The 2nd Global Leadership Initiative Special Workshop on Water Virology　2014/01
Stability of rotavirus infectivity analyzed by oxidative stress marker detection International-presentation

Daisuke Sano

The 5th European Rotavirus Biology Meeting　2013/10
ノロウイルス〜MBRにおける除去性、感染リスク管理、及び性能基準 Invited

佐野大輔

3. 平成25年度先端膜工学研究推進機構秋季講演会・膜工学サロン　2013/09/10
A hidden relationship between norovirus and enteric bacteria: norovirus exploits histo-blood group antigen-like substances on bacterial cells for its better survival in nature? International-presentation

SANO Daisuke

The 47th Joint Working Conference on Viral Diseases, US-Japan Cooperative Medical Science Program　2013/03/13
Development of a novel photocatalytic nano-composite membrane to create new water resources from wastewater International-presentation

Daisuke Sano

SJ-NANO2013 (Spanich-Japanese Bilateral Workshop)　2013/03/05
Performance target of enteric virus removal with MBR International-presentation

Daisuke Sano

Global Leadership Initiative Special Workshop on Water Virology　2013/01/19
Multiple peak expression of enteric virus density in environmental water International-presentation

Daisuke Sano

The 4th Asia-Pacific Young Water Professionals Conference 2012　2012/12
Infectivity of gastroenteritis viruses analyzed by oxidative stress marker detection and RNase sensitivity test International-presentation

Daisuke Sano

ISFEV2012　2012/10
Human norovirus-binding enteric bacteria bearing histo-blood group antigen-like extracellular polymeric substances International-presentation

SANO Daisuke

The 46th Joint Working Conference on Viral Diseases, US-Japan Cooperative Medical Science Program　2012/06/19
Accumulation of oxidative damages on rotavirus capsid protein and its relationship with infectivity International-presentation

Daisuke Sano

The 2nd COST 929 Symposium, Istanbul, Turkey,　2010/10/07
Human enteric bacteria that capture norovirus particles with a specific interaction through histo-blood group antigen-like moiety International-presentation

Daisuke Sano

The 2nd COST 929 Symposium, Istanbul, Turkey　2010/10/07
Norovirus-binding bacteria: significance in the environmental dissemination of gastroenteritis viruses International-presentation

Daisuke Sano

The 44th Joint Working Conference on Viral Diseases, US-Japan Cooperative Medical Science Program, Sapporo, Japan, June 28-29.　2010
Detection of oxidative damages on capsid proteins of norovirus: a new approach to evaluate the infectivity of non-culturable virus

Daisuke Sano

The 15th Symposium on Health-Related Water Microbiology　2009
Waterborne viral gastroenteritis - could prevention be within our reach? International-presentation Invited

Daisuke Sano

Academie Des Sciences-JSPS Workshop on Environment and Health Challenges, Paris, France. May 28-29.　2009
A new approach to evaluate the infectivity of non-cultivatable viruses International-presentation

Daisuke Sano

The 43rd Joint Working Conference on Viral Diseases, US-Japan Cooperative Medical Science Program, Philadelphia, Pennsylvania, USA, July 20-22.　2009
培養できない腸管系ウイルス不活化評価を目的とした外殻タンパク質酸化傷害検出手法の開発

佐野大輔

第46回環境工学研究フォーラム　2009
New detection method for enteric viruses in digestive diverticulum of Pacific oyster, Crassostrea gigas utilizing enzymes for virus elution

The 15th Symposium on Health-Related Water Microbiology　2009
Quantification and molecular characterization of norovirus, sapovirus and hepatitis A virus in the Llobregat river basin (Catalonia, NE Spain)

The 15th Symposium on Health-Related Water Microbiology　2009
Genetic diversity and evolution in amino acid sequences of norovirus genogroup II strains in environmental and clinical samples

The 15th Symposium on Health-Related Water Microbiology　2009
Research activities on waterborne pathogenic viruses in Japan International-presentation Invited

Daisuke Sano

Seminar of molecular microbiology and virology　2007/06/26
Quantification of human astroviruses using a one-step real-time quantitative RT-PCR International-presentation

Daisuke Sano

The 14th Symposium on Health-Related Water Microbiology (WaterMicro07)　2007
Affinity isolation of norovirus-binding proteins from activated sludge microorganisms using a surface-exposed part of viral capsid protein

The 14th Symposium on Health-Related Water Microbiology (WaterMicro07)　2007
水中病原ウイルスの新規検出技術に用いる固定化ウイルス吸着タンパク質の活性評価

平成17年度土木学会東北支部技術研究発表会　2006
ファージディスプレイ法を用いた凝集阻害誘因タンパク質をコードする藻類遺伝子の分離

第43回環境工学研究フォーラム　2006
Seasonal variation of Norovirus occurrence in water environment

The 5th World Water Congress of International Water Association　2006
活性汚泥細菌ゲノムDNAライブラリから取得した新規重金属吸着タンパク質遺伝子

第42回環境工学研究フォーラム　2005
Heavy metal-binding proteins from metal-stimulated bacteria as a novel adsorbent for metal removal technology International-presentation

Daisuke Sano

The 1st IWA-ASPIRE Conference & Exhibition　2005
Membrane separation of indigenous Norovirus from sewage sludge and treated wastewater International-presentation

Daisuke Sano

The 13th Symposium on Health-Related Water Microbiology (WaterMicro05)　2005
アフィニティクロマトグラフィによる活性汚泥細菌からのアデノウイルス吸着タンパク質（Adenovirus-Binding Protein: ADVBP）の分離

第59回土木学会年次学術講演会　2004
アデノウイルス吸着タンパク質（Adenovirus-Binding Protein: ADVBP）の活性汚泥細菌からの分離

第41回環境工学研究フォーラム　2004
活性汚泥細菌から分離されたウイルス吸着タンパク質とポリオウイルス粒子間の相互作用評価

平成14年度土木学会東北支部技術研究発表会　2003
Detection of infectious pathogenic viruses in water and wastewater samples from urbanized area

The 12th Symposium on Health-Related Water Microbiology　2003
Functional Metal-Binding Proteins by Metal-stimulated Bacteria for the Development of an Innovative Metal Removal Technology International-presentation

Daisuke Sano

The 3rd World Water Congress of International Water Association　2002
Enhanced virus recovery from municipal sewage sludge with the combination of enzyme and cation exchange resin International-presentation

Daisuke Sano

The 1st World Water Congress of International Water Association　2000
Enumeration of enteric viruses in municipal sewage sludge with enzymatic virus elution method. International-presentation

Daisuke Sano

Asian Waterqual '99, 7th IAWQ Asia-Pacific Regional Conference　1999

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Research Projects 43

WASH-related diarrhea risk structure and climate change impact in African peri-urban low-income areas

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Kyoto University

2024/04/01 - 2028/03/31
水中病原体群と指標群の相関解析アルゴリズムの開発

加藤毅, 佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 群馬大学

2022/04/01 - 2026/03/31
Early detection of unknown viral mutants that potentially cause COVID-19-like pandemics using from wastewater

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2022/04/01 - 2025/03/31
脱炭素化に向けた創エネ・リン資源回収型下水高度処理システムの開発

李玉友, 北條俊昌, 安井英斉, 増田周平, 久保田健吾, 佐野大輔, 覃宇

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(A)

Institution: 東北大学

2022/04/01 - 2025/03/31
Establishment of a Platform for Pathogenic Virus Surveillance and Infection Risk Control Using Sewage Epidemiology in Developing Asian Countries

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: The University of Tokyo

2022/04/01 - 2025/03/31
都市下水から薬を創り出す：棄てられたヒト免疫細胞由来遺伝子を活用した抗体医薬開発

佐野大輔, 野地智法, 斉藤繭子, 久保田健吾

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 挑戦的研究(開拓)

Institution: 東北大学

2021/07/09 - 2025/03/31

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本研究は、都市下水から回収した抗体遺伝子を用いて抗体医薬の合成を行う手法の開発に取り組むものである。研究初年度である令和3年度においては、文献情報をもとに免疫細胞由来遺伝子を増幅するためのプライマーを設計し、都市下水から抽出した総DNAを鋳型として用いたPCRを行い、都市下水から免疫細胞由来遺伝子の増幅産物を取得することを試みた。都市下水は3000xgで30分遠心分離し、得られた沈殿物を下水濃縮サンプルとした。プライマーは複数の組み合わせを合成し、試行錯誤的に下水濃縮サンプルから抽出した総DNAに適用した。その結果、目的遺伝子と思われる増幅産物を得ることに成功した。得られた増幅産物に対して配列解析を行ったところ、ヒト免疫グロブリン由来の遺伝子配列が得られていることが確認された。この結果により、都市下水中に免疫細胞由来遺伝子が存在していることが証明され、本研究のコンセプトが成立可能であることが示された。続いて、得られた増幅産物に対して次世代シーケンシングを行うための前処理を行なった。最終的に、十分な量と純度を持つサンプルが得られたため、次世代シーケンス解析を開始した。
水の消毒処理に対するウイルスの耐性―遺伝的要因の解明による安全な水利用の実現―

佐野大輔, 片山浩之, 片山和彦, 北島正章

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(A)

Institution: 東北大学

2021/04/05 - 2025/03/31

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研究初年度である令和3年度においては、世界中で水の消毒に使用されている塩素消毒について、感受性の異なるロタウイルス集団を計40集団取得し、塩素消毒実験結果をもとに、17の低感受性（高抵抗性）集団、及び23の高感受性（低抵抗性）集団に分割した。これらのロタウイルス集団に対して次世代シーケンシングによる全ゲノム解析を行ったところ、低感受性集団に特徴的な遺伝子変異は存在しないことが明らかとなった。そこで低感受性集団と高感受性集団の間で遺伝的多様性を比較したところ、外殻タンパク質VP7のアミノ酸配列において、低感受性集団の方が高感受性集団よりも有意に多様性が高いことが判明した。遺伝的多様性が高い場合に集団内で粒子間の凝集が生じやすくなり、それが消毒耐性をもたらすとの仮説を立て、進化シミュレーションにより再現を試みたところ、凝集しやすい集団ほど、消毒耐性がランダムに変動しつつ遺伝的多様性が高く保たれることが明らかとなった。この結果から、代表的な胃腸炎ウイルスであるロタウイルスに関しては、遺伝的多様性自体が集団として消毒剤への低感受性を示す理由であることが示唆された。
スリランカにおける下水水質情報に基づいた下水管路劣化予測システムの開発

佐野大輔, 水谷大二郎, Amarasiri Mohan

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 国際共同研究加速基金(国際共同研究強化(B))

Institution: 東北大学

2021/10/07 - 2024/03/31

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本研究は、スリランカにおいて、微生物の起源を同定する手法であるMicrobial Source Tracking（MST）の手法を応用し、下水管の破損部分から混入する土壌由来の微生物の下水中に占める割合を空間的・時間的に把握することで、下水管破損箇所を推定し、かつ下水管劣化速度の予測に適用するための新規手法を開発することを目指すものである。研究初年度である令和3年度においては、まずスリランカ側カウンターパート（チャンディカ・ガマゲ（ペラデニヤ大学医学部・講師）、及びチャミンダ・サマラスーリヤ（モラトゥワ大学地球資源工学部・講師））とのオンライン打ち合わせを通じ、スリランカ国内における調査地点の確定を行った。希望する調査地点で下水採取を行うために、所轄官庁向けの要望書を作成・提出したところである。さらに、本研究で想定している手法が適用可能であることを確認するために、日本国内で未処理下水を採取し、総DNAを抽出して、細菌の分類に用いられる16s rRNA遺伝子配列の取得を行った。その結果、下水に特徴的な細菌種の16s rRNA遺伝子配列が確実に得られることを確認することに成功し、スリランカで得られたサンプルに適用する準備が整えられた。
Development of new photochemical reaction model for predicting water quality transformation efficiency

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tokyo Institute of Technology

2021/04/01 - 2024/03/31
Invasion of antibiotic resistance genes persisting in aquatic environments to humans: invasion mechanisms and the risk

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Ehime University

2020/04/01 - 2024/03/31
Wastewater monitoring for hepatitis A and E viruses and drugs without approval or authorization and their risk assessment

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: National Institute of Public Health

2020/04/01 - 2023/03/31
機械学習による水中病原体と指標微生物の濃度相関解析法の精緻化

加藤毅, 佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 群馬大学

2019/04/01 - 2023/03/31

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環境水や飲用水には感染症を引き起こす病原体が含まれることがある．病原体とは病原細菌，病原ウイルス，および原虫を含む．モニタリングされる指標微生物の水質衛生基準値を設定する際には，指標微生物濃度と病原体濃度との相関関係を適切に定量化することが求められる．しかし，病原体の陽性率の低さが相関計算の障害となっている．本研究では，近年著しい発展を遂げる機械学習を使うことで，指標微生物と病原体の濃度相関解析を改善することを目指した．従来は指標微生物や病原体の濃度を使って相関を計算してきた．これに対し，本研究は，指標微生物や病原体の濃度と同時に取得できる水質データやドメイン知識を利活用することで，相関解析法の高精度化を狙った．統計学において，トビット法という，非定量値と回帰直線を同時に推定する方法がある．トビット法では，観測された病原体濃度の確率密度関数，および検出限界を下回った濃度がその確率モデルにおいて非観測となる確率質量関数を得ることが出来，それらを組み合わせることで回帰係数の尤度関数を構成する．このアプローチをそのまま使ってしまう場合，予測に用いる情報が不十分になるため，非検出値の予測分布は大雑把なものにしかならず，そこから得られる相関解析は十分な精度で得られない．本研究では，水質データの援用によって，非検出値の予測分布を精密にすることで，相関係数の精度を向上させた．個別の説明変数の予測能力はさほど強くないため，標本が小さいとき符号が逆転した標本相関が発生する．本研究では，ドメイン知識を符号制約で表すことで，標本が小さくても，回帰分析の精度を大きく改善させ，これを相関解析のワークフローに取り込むことで，相関解析の精度を向上させることに成功した．
下水再生利用におけるエネルギー回収と健康リスク管理の実現

Offer Organization: 国立研究開発法人科学技術振興機構

System: 日本－中国国際共同研究イノベーション拠点共同研究

Institution: 東北大学

2019/04 - 2022/03
The Sanitation Value Chain: Designing Sanitation Systems as Eco-Community-Value System

Offer Organization: Research Institute for Humanity and Nature

System: Research Project

Institution: Research Institute for Humanity and Nature

2015/04 - 2022/03
Development of nanocarbon-TiO2 composite showing high self-regeneration capacity for water treatment

Yoshimura Chihiro

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tokyo Institute of Technology

2018/04/01 - 2021/03/31

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To remove persistent organic compounds and pathogenic microorganisms from water, we developed simple methods for preparing nanocarbon materials, optimized those composites with metal catalysts, and elucidated their reaction characteristics. The optimized composite material of magnetic carbon nanotubes and titanium dioxide was further investigated on its performance in secondary treated water of sewage, which led to elucidation and modeling of adsorption and photocatalytic degradation of organic pollutants and microbes.
ノロウイルスの水環境中キャリアーとなる糖鎖分泌細菌の多様性と存在実態の解明 Competitive

佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費補助金・基盤研究A

2017/04 - 2021/03
衛生安全計画に基づいた下水処理放流水質の衛生工学的管理スキームの構築

Offer Organization: 国土交通省

System: 下水道技術研究開発

Institution: 東北大学

2018/10 - 2021/02
Why noroviruses are accumulated in oysters: To unravel the mechanisms

Omura Tatsuo

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Pioneering)

Institution: Tohoku University

2017/06/30 - 2020/03/31

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The dynamics of norovirus, one of the causative agent of infectious gastroenteritis, in the estuarine environment is not well understood. In this study, based on the hypothesis that planktons in seawater can be the important carrier of norovirus to oysters, field study was conducted. Norovirus GII RNA were detected from sorted zooplankton and phytoplankton samples, and oyster digestive tissues. The maximum concentrations of norovirus GII RNA were 3900 copies/L in plankton samples, 58 copies/individual of zooplankton, and 2000 copies/g of oyster digestive tissues. DNA metabarcoding analysis indicated that oysters prey on zooplankton (phylums Cercozoa and Ciliophora) and phytoplankton (phylum Chlorophyta, and Ranthkea_octopunctata). Correlation of norovirus GII with some specific planktonic species (e.g., Rathkea_octopunctata, Sarsia_tubulosa) were found, implying the important roles of such species as potential norovirus carrier.
Analysis of salt-resistant Escherichia coli strains using transcriptome analysis

Masago Yoshifumi, INABA Manami

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

2015/04/01 - 2018/03/31

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This study tested a hypothesis that a part of Escherichia coli in coastal area acquires resistance against salt (sodium chloride), and they cannot be detected using ordinary selection media”. A field survey at a coastal area in Japan found higher concentration of E. coli using selective agar supplemented with salt compared to the ordinary agar in summer season. But the salt-resistant E. coli strains isolated from the water samples showed low growth rates in salt-supplemented media, indicating that salt inhibits growth of salt-resistant E. coli strains. These finding implies that E. coli in coastal water shows lower overall decay rates due to the strains possessing salt-resistance.
Development of system for real-time prediction of pathogen level

Kato Tsuyoshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Gunma University

2015/04/01 - 2018/03/31

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To ensure the microbial safety in water usage, it is meaningful to prediction pathogens on a real-time basis. The concentration of pathogens in water, which is measured by culture-dependent methods, has been used to monitor the fecal contamination in water environment, and has been proved to be effective to prevent waterborne infectious diseases in varied water usage styles. On the other hand, the real-time monitoring has not yet been achieved for most of pathogens. In this project, we exploit the fact that it is possible to measure some of the hydrological and water quality data with real-time sensors, for the purpose of the real-time prediction of pathogens by using these hydrological and water quality data as explanatory variables. The methodology was devised by combining the state-of-the-art mathematical theories with the domain knowledge in the water quality engineering. Online software was developed so that any people can use the methodology.
Environmental adaptive evolution of waterborne viruses under water recycling systems

Omura Tatsuo

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tohoku University

2015/04/01 - 2018/03/31

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We investigated the adaptive evolution of gastroenteritis viruses under the repeated disinfection with free chlorine. Murine norovirus (MNV) and Rhesus rotavirus (RRV) were used as surrogates for human norovirus and rotavirus. As a result, resistant populations of these viruses to free chlorine were successfully acquired by the repeated treatment with free chlorine. The single nucleotide polymorphisms revealed that specific mutations were found in outer capsid proteins of these viruses, which implied that some mutations in structural proteins may contribute to the virion stability and lead to the change in free chlorine susceptibility. Based on these results, it was concluded that the free chlorine disinfection can be regarded as a selection pressure on gastroenteritis viruses.
培養細胞のアポトーシスを指標とした下水再生水のウイルス感染リスク評価技術の開発 Competitive

佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費補助金・挑戦的萌芽研究

2016/04 - 2018/03
腸内細菌由来糖鎖が小腸上皮細胞への胃腸炎ウイルス感染に与える影響に関する研究 Competitive

佐野大輔

Offer Organization: 武田科学振興財団

System: ライフサイエンス研究奨励

2015/10 - 2017/09
Rapid detection of infectious enteric viruses in water using cellular responses to virus infection

Omura Tatsuo, DAISUKE SANO, YOSHIFUMI MASAGO

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2014/04/01 - 2016/03/31

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This study aimed to identify candidate genes for detecting infectious viruses when concentrated water samples were inoculated to cultivated human cell lines. A vaccine strain of poliovirus type 1 (PV1) was used as a test virus, and the INT407 cell line derived from human intestinal epithelial cells was used as a host cell line. The expression level of KCNJ4 and SCN7A genes were monitored in the post-infection of PV1. When PV1 was inoculated at multiplicity of infection (MOI) = 1, the expression levels of KCNJ4 and SCN7A genes were significantly increased compared to those in mock cells. These results indicate that these genes of ion-chancel proteins are available as cellular genetic markers for detecting the presence of infectious viruses in water samples.
Elucidation of the fate of human noroviruses in water environments

TATSUO Omura, HARADA Hideki, SANO Daisuke, WATANABE Toru

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tohoku University

2012/04/01 - 2015/03/31

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In this study, we confirmed that the oxidative stress marker detection of viral capsid protein can be applied to the infectivity evaluation of human noroviruses in water environments when 4-log reduction of infectious titer is expected. Furthermore, a novel methodology to recover intact viral genomic RNA from environmental samples was newly established, in which only the target viral genomic RNA can be specifically recovered. In the investigation of cultivated oysters about norovirus contamination, we applied next generation sequencing technology to identify norovirus genotypes contaminatinng cultivated oysters, and found that not only predominant genotypes but laos minor strains can be detected.
先進国社会における胃腸炎ウイルス適応進化による消毒剤耐性獲得メカニズムの解明 Competitive

佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費補助金・挑戦的萌芽研究

2013/04 - 2015/03
組織血液型決定抗原様物質を保持する腸内細菌がヒトノロウイルスの環境中動態に与える影響の解明 Competitive

佐野大輔

Offer Organization: 発酵研究所

System: 一般研究助成

2014/04 -
グライコミクス情報を活用した下水処理水再利用における胃腸炎ウイルス制御の新展開 Competitive

佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費補助金・基盤研究A

2014/04 -
日本で発生しうる輸入感染症予見を目的としたスペインにおける下水中病原微生物の同定 Competitive

佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費補助金・基盤研究B

2014/04 -
糞便汚染マーカー定量検出を基盤とした微生物学的水質管理手法の確立

岡部聡, 石井聡, 佐藤久, 佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(A)

Institution: 北海道大学

2011/04/01 - 2014/03/31

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現行の糞便汚染指標である大腸菌群数による微生物学的水質管理は、水環境中での大腸菌群の増殖、糞便に由来しない大腸菌群の存在など、数多くの問題点が指摘されている。このため、合理的な糞便性汚染指標の確立および水域の微生物学的水質管理手法の確立が急務となっている。このような背景のもと、本研究では、宿主特異的遺伝子マーカー（腸内蛋白質分解細菌の最優占種であるBacteroides-Prevotella 属由来遺伝子）をもとに水域の糞便汚染レベルを定量的に評価し、さらに糞便汚染源（ヒト、家畜及び野生動物等）を迅速かつ正確に特定する新規方法論を確立し、具体的な汚染防止対策の構築を含む合理的な微生物学的水質管理を実現することを目的としている。本年度は、昨年度開発した各宿主特異的糞便汚染マーカーを実際の水環境に適用し、糞便汚染の実態を明らかにした。さらに、遺伝子マーカーを糞便汚染指標として活用するためには、生存細胞と死細胞を区別して定量することが求められる。そこで、Propidium monoazide (PMA)を併用した定量PCR法を確立し、糞便汚染源の特定を行うために重要となる生菌由来の糞便性汚染マーカーの定量が可能となった。次に、環境水中における糞便汚染マーカー（ヒト、ブタ、ウシ、ニワトリ、カモの各宿主特異的遺伝子マーカー）の挙動を解析した。さらに、既存の糞便汚染指標である大腸菌群数、糞便生大腸菌群及び大腸菌の定量も行い、減衰速度の違いを評価した。最後に、水系感染する腸管系感染症起因細菌及びノロウイルス等の腸管系ウイルスの特異的検出を行い、各宿主特異的遺伝子マーカーと病原微生物の環境水中における存在比の相関関係を調査した。
腸内細菌由来糖鎖を活用したノロウイルスの水環境中動態解明に関する研究 Competitive

佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費補助金・若手研究A

2009/04 - 2014/03
Challenges to replicate human norovirus with tissue cells

OMURA Tatsuo, SANO Daisuke, MASAGO Yoshifumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Tohoku University

2011 - 2012

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The main purpose of this project was to acquire precious knowledge for establishing the infection of human norovirus to tissue cells. We successfully constructed a norovirus-like particle with green fluorescence protein (GFP-NoVLP). GFP-NoVLP was applied for confirming the intrusion and decapsidation of human norovirus in tissue cells, but it was found that the amount of fluorescence was not enough to observe the movement of GFP-NoVLP in tissue cells. Therefore, we paid attentions to cellular responses of tissue cells against the virus infection, and tried to identify genetic markers available for the detection of virus infection. Poliovirus type 1 (PV1) Sabin strain was used as a test virus, and a transcriptome analysis of INT407 cells infected with PV1 was conducted. As a result, KCNJ4 gene, responsible for potassium homeostasis, was found as the most promising genetic marker for the detection of virus infection.
Development of a sensor for detection of pathogenic virus in water based on fluorescent molecule and virus binding protein

SATOH Hisashi, SANO Daisuke

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Exploratory Research

Institution: Hokkaido University

2010 - 2011

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The final goal of this study is to develop a sensor for detection of pathogenic virus in water. In this study, we synthesized virus-binding proteins(VBP) and fluorophore. VBP were produced by genetically-modified E. coli. The SDS-PAGE profile showed the extracted soluble proteins from E. coli cells were VBP of which the estimated molecular weight is 57 kDa. We could also synthesize fluorophore(4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene) in our laboratory
Development of virus-binding protein-based technologies for concentration, detection and identification of pathogenic viruses in water environment

OMURA Tatsuo, KATAYAMA Hiroyuki, SANO Daisuke, WATANABE Toru, MASAGO Yoshifumi, UEKI Yo

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (S)

Institution: Tohoku University

2007 - 2011

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The objective of this project was to develop novel technologies for recovery, detection and identification of pathogenic viruses in water environments by taking advantages of our own approaches including virus-binding protein (VBP)and enzymatic virus elution (EVE)method. As a result, enteric virus-binding protein (EVBP)that can capture diverse enteric viruses was successfully identified, and novel methodologies for virus recovery from environmental matrixes such as river and estuarine sediments were established. Furthermore, a novel methodology for detecting infectious pathogenic viruses in water by using ethidium monoazide was also developed. These achievements allow us to complete the original targets of this project.
先進国社会における特異的な水系感染症ウイルス進化による塩素耐性獲得メカニズム解明 Competitive

佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費補助金・研究活動スタート支援

2009/04 - 2010/03
コミュニティーレベルでの胃腸炎ウイルス制御法開発(社会のワクチン)への挑戦

中込治, 中込とよ子, 佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 挑戦的萌芽研究

Institution: 長崎大学

2009 - 2009

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ノロウイルスはどうやって現代の上下水処理制御をかいくぐり自然界でヒトからヒトへの感染環を維持しているのか。ノロウイルスによる被害を最小化するためには、ノロウイルスの自然界での動態を明らかにし、感染制御をコミュニティーレベルで達成する新規な衛生工学的水処理技術の開発が必要である。本研究は、ノロウイルスが組織血液型抗原をレセプターとすることにヒントを得て、ノロウイルスを環境中で安定に生存させるキャリア細菌があるのではないかという仮説を検証することにより、新規な下水処理法の開発につながる突破口をつかむことを目的とした。未処理下水を原材料に6種類の腸内細菌用培地を用いた微好気性培養を行い、これを血液型判定法の原理を応用したELISAによりノロウイルス吸着細菌をスクリーニングした。ここで分離された菌を微好気条件下に単離し、増菌後に組織血液型抗原への結合を確認した上で、16SrDNAの配列を決定し、ホモロジー検索によりPseudomonas,Klebsiella,Proteusに近縁な3株を同定した。これらの3株と対照菌株である大腸菌K12株とを用いて、ノロウイルス粒子の吸着実験を行ったところ、吸着平衡定数が10^12のオーダーの高い値が得られた。すなわち、菌体表面に組織血液型抗原と同等の糖鎖構造をもち、かつ高い平衡定数でノロウイルスに吸着する腸内細菌株を見出すことができた。今後、再現性および推定パラメータの精度確認などの実験が必要であるが、ノロウイルスのキャリア細菌仮説を強く支持する結果が得られたことから、コミュニティーレベルでの胃腸炎ウイルス制御法開発,すなわち,「社会のワクチン」開発に向けての第1歩が得られたと考える。
ウイルス吸着タンパク質と光触媒の複合化による水中病原ウイルス除去・不活化技術開発 Competitive

佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費補助金・特別研究員奨励費

2004/04 - 2007/03
The elucidation of inactivation mechanisms of Noroviruses with the detection of damages on viral gene and capsid protein Competitive

2007 -

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The investigation of norovirus, one of important causative agents for viral diseases, has a big burden that this virus can not grow with cell culture in laboratory. This means that it is impossible to evaluate the norovirus inactivation by disinfection techniques, such as chlorine treatment and ultraviolet radiation. In this study, a new method to directly detect damages on noroviral particle will be developed, which could contribute to the elucidation of the mechanism of norovirus inactivation.
Development of new technology for removing viruses from water environment

OMURA Tatsuo, WATANABE Toru

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: Tohoku University

2004 - 2006

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Virus-binding proteins (VBPs) are bacterial proteins to which human viruses preferentially adsorb in water environment. VBPs were discovered from a bacterial culture derived from activated sludge with affinity chromatography assay in which a viral capsid peptide was used as a ligand. The purpose of this study was to develop a novel technology using VBPs for virus removal from environmental water. Achievements described below were obtained during the 3-year study duration. At the first year of the study duration, the scale-up of VBP production and purification was conducted in order to obtain large amount of VBP. A handmade column for VBP purification was constructed, which made it possible to obtain about 10 times larger volume of VBP than conventional procedure. At the second year, gene of hexalysine-tagged VBP (LysTagVBP), which has 6-consecutive lysine residues in its C terminus, was fabricated with PCR, and used for production of LysTagVBP with E. coli. Then, polylysine region in LysTagVBP was coupled with the surface of glass particles using silane coupling reagent and glutaraldehyde. The creation of LysTagVBP directly linked to the effective immobilization of VBP on carrier surface. At the third year of the study duration, the virus-removing column in which LysTagVBP was immobilized was constructed, and virus removal efficiency with the column was evaluated. As a result, about 40% of viruses were reduced during the first 1-hour operation, and 70% of viruses were removed after the 3-hour operation. The development of virus removal technology using VBPs is a totally new challenge, and the achievements with regards to the utilization of VBPs as viral adsorbent would be useful in the farther application of VBPs to virus removal from water.
Investigation on the Growth Mechanism of the Toxic Cyanobacterium (Lyngbya majuscula) in Moreton Bay, Queensland, Austraria

OMURA Tatsuo, WATANABE Toru

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (A)

Institution: Tohoku University

2004 - 2006

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We have investigated on the behavior of iron in coastal waters and iron uptake mechanism of toxic cyanobacterium, Lyngba majuscula, and developed a numerical model to predict the dynamics of bioavailable iron in coastal waters. Follows are major outcomes from this study. 1.Laboratory experiment and field work were conducted to understand forms of iron in coastal water and behavior of humic iron complex such as equilibrium and coagulation. It was found that ferric iron complexed by humic substances (HS) is predominant iron species in river water, however, 90 % of the complex dissociates in coastal water due to the competition with divalent seawater cations (i.e.,Ca^<2+> and Mg^<2+>). Chromatography analysis revealed that coagulation was least for HS which has low molecular weight and hydrophobicity, indicating that HS having these properties are the most important contributor to transport dissolved iron from river to coastal waters. 2.The numerical model to predict behavior of humic iron complex was developed, considering the physicochemical behavior of humic iron complex such as equilibrium, coagulation and diffusion. The equilibrium model was developed on the basis of Debye-Huckel theory that is able to take account of the potential energy of iron around the surface of HS. The coagulation model was developed by involving hydrophobolic interaction, a strong attractive force working among HS molecules, into the well established DLVO theory. The equilibrium and coagulation model was then combined with diffusion model (Navier-Stokes and diffusion equations), allowing us to numerically calculate the spatial and temporal distributions of humic iron complex in coastal water. 3.The kinetics of bioavailable iron production via superoxide-mediated reactions was investigated to develop a model for the iron uptake by L. majuscula. Although the rate of superoxide-mediated production of bioavailable Fe(II) (i.e., bioavailability of iron) varies due to the different origin of humic iron complex, the humic iron complex with higher content of acid functional groups generally shows lower bioavailability. By determining the kinetic rate constants involved in Fe(II) production, reaction scheme in terms of iron uptake was developed. The model calculated that the strong humic iron complex produces Fe(II) via non-dissociative reduction pathway in which the Fe(III) complexed by HS is reduced by superoxide prior to the complex dissociation. The results indicated that management and environment of catchments such as land use and vegetation (i.e.,origin of HS) significantly affect the bioavailability of iron in coastal waters.. In summary, the numerical model which involves the equilibrium, coagulation, diffusion and bioavailability of humic iron complex was developed in this work. The model allows us to comprehensively assess and predict the process of iron uptake by L.majuscula in coastal waters.
都市域内高度水循環システム構築にための下水処理水からの病原ウイルス除去技術開発 Competitive

佐野大輔

Offer Organization: 日本学術振興会

System: 科学研究費補助金・特別研究員奨励費

2002/04 - 2004/03
Development of virus detection method using virus-binding protein Competitive

System: Grant-in-Aid for Scientific Research

2000 -

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Virus-binding proteins are bacterial proteins that can exhibit the binding ability with human pathogenic viruses. It has been speculated that the presence of virus-binding proteins affects the movement of pathogenic viruses in water environment. In this study, a new method for the detection of viruses from water using the virus-binding protein as viral adsorbent is going to be developed.
The elucidation of virus transmission pathway in water environment Competitive

System: Grant-in-Aid for Scientific Research

1997 -

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It has been elucidated that some pathogenic viruses frequently causing infectious diseases among human are contaminating water environment via treated wastewater. The water environment could have an important role in the transmission of pathogenic viruses in human society, but details have remained to be clear. In this study, in order to elucidate the mechanisms of virus transmission in human society, effective virus detection methods for variety of water samples, including river water, sea water and sediments, are newly developed, and they are applied to the actual environmental water samples to know the pathway of pathogenic viruses in water environment.

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Teaching Experience 6

Water and Public Health　Faculty of Engineering, Hokkaido University
工学基礎演習　北海道大学環境社会工学科
水・公衆衛生特論　北海道大学大学院工学研究院
環境工学序論　北海道大学工学部環境社会工学科
物理化学要論　北海道大学工学部環境社会工学科
分離工学　北海道大学工学部環境社会工学科

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