Details of the Researcher

PHOTO

Nobuhiro Takahashi
Section
Graduate School of Dentistry
Job title
Specially Appointed Professor(Research)
Degree

  • 歯学博士（東北大学）

Research History 14

  • 2025/04 - Present
    Tohoku University

  • 2025/04 - Present
    東北大学大学院歯学研究科　特任教授

  • 2001/03 - 2025/03
    東北大学大学院歯学研究科　口腔生化学分野　教授

  • 2020/04 - 2023/03
    東北大学大学院歯学研究科　研究科長・学部長

  • 2010/04 - 2023/03
    Tohoku University

  • 2009/02 - 2020/03
    東北大学大学院歯学研究科　副研究科長・副学部長

  • 2010/04 - 2012/03
    Tohoku University

  • 2004/04 - 2008/03
    東北大学大学院歯学研究科　副研究科長・副学部長

  • 2000/04 - 2001/02
    東北大学大学院歯学研究科　口腔生化学分野　助教授

  • 1998/04 - 2000/03
    東北大学歯学部　口腔生化学講座　助教授

  • 1990/05 - 1998/03
    東北大学歯学部　口腔生化学講座　助手

  • 1988/05 - 1990/04
    ミネソタ大学歯学部　Visiting Assistant Professor

  • 1988/04 - 1988/04
    東北大学歯学部附属病院 口腔外科　医員

  • 1986/04 - 1988/03
    日本学術振興会　特別研究員DC

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Education 2

  • 東北大学大学院歯学研究科

    1984/04 - 1988/03

  • 東北大学歯学部

    1978/04 - 1984/03

Committee Memberships 17

  • 東北大学創薬戦略推進機構基礎探索研究PF推進室　室員

    2024/12 - Present

  • 宮城県歯科保健推進協議会　会長

    2023/04 - Present

  • 大学基準協会歯学教育評価委員会　委員

    2023/04 - Present

  • 口腔保健用機能性食品研究会　理事長

    2020/01 - Present

  • 東北大学未来型医療創造卓越大学院プログラム未来型医療創造教育研究センター教務委員会　副委員長

    2019/04 - Present

  • 東北大学未来型医療創造卓越大学院プログラム未来型医療創造教育研究センター運営委員会　副委員長

    2019/04 - Present

  • 口腔保健用機能性食品研究会　理事

    2011/08 - Present

  • 国際歯科研究学会日本部会（JADR）　理事

    2003/01 - Present

  • 歯科基礎医学会　理事

    2002/04 - Present

  • 日本生化学会　評議員

    2001/04 - Present

  • 歯科基礎医学会　評議員

    2001/04 - Present

  • 東北大学歯学会　評議員

    2001/04 - 2025/03

  • International Association for Dental Research　Regional Board Member

    2021/07 - 2024/03

  • 東北大学歯学会　会長

    2020/06 - 2023/05

  • 日本口腔衛生学会　代議員

    2018/04 - 2022/03

  • 国際歯科研究学会日本部会（JADR）　会長

    2013/01 - 2014/12

  • 日本齲蝕学研究会議　理事

    1999/04 - 2010/03

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Professional Memberships 7

  • 日本う蝕研究会議(1999/04- 理事)

  • 日本生化学会(2001/04- 評議員)

  • 国際歯科研究学会日本部会（JADR）(2003/01- 理事)

  • 歯科基礎医学会(2002/04- 理事)

  • 歯科基礎医学会(2001/04- 評議員)

  • 東北大学歯学会(2001/04-2003/03 理事)

  • 東北大学歯学会(2001/04- 評議員)

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Research Interests 6

  • 生体材料の微生物学的劣化

  • 低酸素細胞生物学

  • 口腔生態系と全身疾患／健康（発がん、高血圧、健康維持・増進）

  • Anaerobic Chambers for Biochemical and Microbiological Experiments

  • Metabolism of Oral Microorganisms and Analysis of Oral Microbial Flora

  • Oral Ecosystem and Oral Diseases (Dental Caries and Periodontal Diseases)

Research Areas 5

  • Life sciences / Oral medicine /

  • Life sciences / Healthcare management, medical sociology /

  • Life sciences / Hygiene and public health (non-laboratory) /

  • Life sciences / Hygiene and public health (laboratory) /

  • Life sciences / Bacteriology /

Awards 3

  1. Yngve Ericsson Prize

    2019/07

  2. 学術奨励賞

    1997/10　歯科基礎医学会

  3. IADR Travel Award

    1993/03　IADR

Papers 215

  1. Profiling of the microbes on the surface of smartphone touchscreens Peer-reviewed

    Nanase Takahashi, Anna Wakui, Yume Sekizawa, Miho Kawachi, Mirai Sekiguchi, Takashi Abe, Aya Sato, Misato Miyazawa, Manami Imai, Nagara Kaku, Shingo Maruyama, Hiroto Sano, Nahoko Kakihara, Jumpei Washio, Yuki Abiko, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of Oral Biosciences　67　(1)　100607-100607　2025/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.job.2024.100607 　

    ISSN： 1349-0079

    eISSN： 1880-3865

  2. Extracellular Z-DNA Enhances Cariogenicity of Biofilm Peer-reviewed

    S. L. Han, J. Wang, H. S. Wang, P. Yu, L. Y. Wang, Y. L. Ou, L. J. Ding, J. Washio, N. Takahashi, L. L. Zhang

    Journal of Dental Research　2025

    DOI： 10.1177/00220345251316822 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  3. Microbiome Signatures and Dysbiotic Patterns in Oral Cancer and Precancerous Lesions Peer-reviewed

    Cheng Chieh Yang, Jumpei Washio, Yu Cheng Lin, Ming Lun Hsu, Ding Han Wang, Fa Tzu Tsai, Yu Min Lin, Hsi Feng Tu, Hsiu Chuan Chang, Nobuhiro Takahashi

    Oral Diseases　2025

    DOI： 10.1111/odi.15317 　

    ISSN： 1354-523X

    eISSN： 1601-0825

  4. Nitrite Production from Nitrate in the Oral Microbiome and Its Contribution to Oral and Systemic Health Peer-reviewed

    Jumpei Washio, Nobuhiro Takahashi

    Advances in experimental medicine and biology　1472　89-101　2025

    DOI： 10.1007/978-3-031-79146-8_6 　

    ISSN： 0065-2598

  5. Candida albicans and NCAC species: acidogenic and fluoride-resistant oral inhabitants Peer-reviewed

    Haneen Raafat Fathi Mousa, Yuki Abiko, Jumpei Washio, Satoko Sato, Nobuhiro Takahashi

    Journal of Oral Microbiology　17　(1)　2025

    DOI： 10.1080/20002297.2025.2473938 　

    eISSN： 2000-2297

  6. The Effect of Environmental Factors on the Nitrate and Nitrite Metabolism of Oral Actinomyces and Schaalia Species Peer-reviewed

    Tomona Otake, Jumpei Washio, Kazuko Ezoe, Satoko Sato, Yuki Abiko, Kaoru Igarashi, Nobuhiro Takahashi

    Molecular Oral Microbiology　40　(2)　104-115　2025

    DOI： 10.1111/omi.12492 　

    ISSN： 2041-1006

    eISSN： 2041-1014

  7. Effects of flushing of dental waterlines in portable dental units on water quality management Peer-reviewed

    Kunihiro Fushimi, Jun Watanabe, Masahiro Yamada, Jumpei Washio, Nobuhiro Takahashi, Hiroshi Egusa

    Journal of Dental Sciences　19　S61-S69　2024/12

    DOI： 10.1016/j.jds.2024.10.014 　

    ISSN： 1991-7902

    eISSN： 2213-8862

  8. Antimicrobial effects of epigallocatechin-3-gallate, a catechin abundant in green tea, on periodontal disease-associated bacteria Peer-reviewed

    Mayu Higuchi, Yuki Abiko, Jumpei Washio, Nobuhiro Takahashi

    Archives of Oral Biology　167　2024/11

    DOI： 10.1016/j.archoralbio.2024.106063 　

    ISSN： 0003-9969

    eISSN： 1879-1506

  9. Molecular microbiological profiling of bottled unsweetened tea beverages: A screening experiment Peer-reviewed

    Nagara Kaku, Miho Kawachi, Anna Wakui, Misato Miyazawa, Manami Imai, Nanase Takahashi, Aya Sato, Takashi Abe, Haruna Sato, Yuki Kato, Rika Okabe, Yuka Naruse, Nao Sato, Nanami Asano, Momoko Morohashi, Hiroto Sano, Jumpei Washio, Yuki Abiko, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of Oral Biosciences　66　(3)　628-632　2024/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.job.2024.07.006 　

    ISSN： 1349-0079

    eISSN： 1880-3865

  10. Effects of nitrate and nitrite on plaque pH decrease and nitrite-producing and -degrading activities of plaque in vitro. International-journal Peer-reviewed

    Yuji Yamamoto, Jumpei Washio, Koichi Shimizu, Nobuhiro Takahashi

    Caries research　2024/07/02

    DOI： 10.1159/000540017 　

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    INTRODUCTION: The purpose of this study was to investigate the effects of nitrate and nitrite on the pH-lowering activity of human plaque, the nitrite-producing and -degrading activities of human plaque, and their correlation. METHODS: Nitrate and nitrite were added to human plaque suspensions collected from the buccal aspect of maxillary molars of patients visiting a general dental clinic, and changes in pH were measured with and without glucose addition. Nitrite-producing and -degrading activities were evaluated by adding nitrate and nitrite to the plaque suspension and measuring the increase and decrease in nitrite with Griess reagent, respectively. RESULTS: The addition of nitrate inhibited both endogenous and glucose-induced plaque pH lowering. The addition of glucose enhanced the production of nitrite from nitrate by about 3.3-fold. The addition of nitrite also inhibited endogenous plaque pH-lowering, but the addition of glucose promoted nitrite degradation by only about 1.1-fold. Nitrite-producing activity was positively correlated with age, but not with nitrite-degrading activity. CONCLUSION: This study revealed that nitrite was produced from nitrate and inhibited the pH-lowering activity of human plaque, which may contribute to caries control. Both nitrite-producing and -degrading activities occurred in human plaque, but no correlation was found between them. Furthermore, nitrite production was enhanced by glucose metabolism, which may function as a self-regulatory mechanism (resilience) to prevent excessive acidification by glucose metabolism.

  11. Electrochemical difference between single‐ and poly‐crystalline LaF3 for a compact and low‐price fluoride ion sensor Peer-reviewed

    Kaito Kanda, Tetsuya Yamada, Yasuko Yanagida, Yuta Kubota, Nobuhiro Matsushita, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi

    Electroanalysis　36　(4)　2024/02/27

    Publisher: Wiley

    DOI： 10.1002/elan.202300318 　

    ISSN： 1040-0397

    eISSN： 1521-4109

  12. Application of omics technologies in cariology research: A critical review with bibliometric analysis Peer-reviewed

    Josie Shizhen Zhang, Shi Huang, Zigui Chen, Chun Hung Chu, Nobuhiro Takahashi, Ollie Yiru Yu

    Journal of Dentistry　141　2024/02

    DOI： 10.1016/j.jdent.2023.104801 　

    ISSN： 0300-5712

  13. Lactic Acid Bacteria in the Human Oral Cavity: Assessing Metabolic Functions Relevant to Oral Health and Disease Peer-reviewed

    Jumpei Washio, Yuki Abiko, Takuichi Sato, Nobuhiro Takahashi

    Methods in molecular biology (Clifton, N.J.)　2851　151-172　2024

    DOI： 10.1007/978-1-0716-4096-8_14 　

    eISSN： 1940-6029

  14. Green Tea-Derived Catechins Suppress the Acid Productions of Streptococcus mutans and Enhance the Efficiency of Fluoride International-journal Peer-reviewed

    Sili Han, Jumpei Washio, Yuki Abiko, Linglin Zhang, Nobuhiro Takahashi

    Caries Research　57　(3)　255-264　2023/11/01

    DOI： 10.1159/000534055 　

    ISSN： 0008-6568

    eISSN： 1421-976X

  15. Questionnaire survey on the satisfaction of SimEx dental education system Peer-reviewed

    Guang Hong, Chun Cheng Hung, Gen Mayanagi, Takashi Nishioka, Lu Sun, Eddie Hsiang Hua Lai, Ting Hsun Lan, Keiichi Sasaki, Nobuhiro Takahashi

    Journal of Dental Sciences　18　(2)　840-847　2023/04

    DOI： 10.1016/j.jds.2023.01.028 　

    ISSN： 1991-7902

    eISSN： 2213-8862

  16. In situ detection of endogenous proteolytic activity and the effect of inhibitors on tooth root surface Peer-reviewed

    Izumi Sakurai, Gen Mayanagi, Satoru Yamada, Nobuhiro Takahashi

    Journal of Dentistry　131　2023/04

    DOI： 10.1016/j.jdent.2023.104471 　

    ISSN： 0300-5712

  17. All-solid-state Fluoride Ion-selective Electrode using LaF<inf>3</inf> Single Crystal with Poly(3,4-ethylenedioxythiophene) as Solid Contact Layer Peer-reviewed

    Tetsuya Yamada, Kaito Kanda, Yasuko Yanagida, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi

    Electroanalysis　35　(4)　2023/04

    DOI： 10.1002/elan.202200103 　

    ISSN： 1040-0397

    eISSN： 1521-4109

  18. Fluoride ion sensor based on LaF3 nanocrystals prepared by low-temperature process Peer-reviewed

    Tetsuya Yamada, Kaito Kanda, Yasuko Yanagida, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi

    Journal of the Ceramic Society of Japan　131　(3)　31-36　2023/03/01

    Publisher: Ceramic Society of Japan

    DOI： 10.2109/jcersj2.22127 　

    ISSN： 1882-0743

    eISSN： 1348-6535

  19. Combined Treatment with Fluoride and Antimicrobial Peptide GH12 Efficiently Controls Caries in vitro and in vivo Peer-reviewed

    Yufei Wang, Yuhao Zeng, Zening Feng, Zhongxu Li, Xuelian Jiang, Sili Han, Jumpei Washio, Nobuhiro Takahashi, Linglin Zhang

    Caries Research　56　(5-6)　524-534　2023/02/01

    DOI： 10.1159/000528057 　

    ISSN： 0008-6568

    eISSN： 1421-976X

  20. Glycolysis-Related Gene Analyses Indicate That DEPDC1 Promotes the Malignant Progression of Oral Squamous Cell Carcinoma via the WNT/β-Catenin Signaling Pathway Peer-reviewed

    Guangzhao Huang, Su Chen, Jumpei Washio, Grace Paka Lubamba, Nobuhiro Takahashi, Chunjie Li

    International Journal of Molecular Sciences　24　(3)　2023/02

    DOI： 10.3390/ijms24031992 　

    ISSN： 1661-6596

    eISSN： 1422-0067

  21. Investigation of drug resistance of caries-related streptococci to antimicrobial peptide GH12 Peer-reviewed

    Xinwei Li, Yufei Wang, Xuelian Jiang, Yuhao Zeng, Xinran Zhao, Jumpei Washio, Nobuhiro Takahashi, Linglin Zhang

    Frontiers in Cellular and Infection Microbiology　12　2022/09/08

    DOI： 10.3389/fcimb.2022.991938 　

    eISSN： 2235-2988

  22. Quantification of Porphyromonas gingivalis Bacteria in Final Trimester of Pregnant Women According to Their Oral Health Status Peer-reviewed

    Retno I. Roestamadji, Udijanto Tedjosasongko, Nuraini Indrastie, Indeswati Diyatri, Meircurius D.C. Surboyo, Subijanto M. Sudarmo, Budi Santoso, Nobuhiro Takahashi

    European Journal of General Dentistry　11　(3)　201-206　2022/09

    DOI： 10.1055/s-0042-1759755 　

    ISSN： 2278-9626

    eISSN： 2320-4753

  23. Profiling of the microbiota of breast milk before and after feeding with an artificial nipple Peer-reviewed

    Hiroto Sano, Anna Wakui, Miho Kawachi, Shingo Maruyama, Sachie Moriyama, Mayumi Nishikata, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Reiko Sakashita, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of Oral Biosciences　64　(4)　431-436　2022/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.job.2022.09.004 　

    ISSN： 1349-0079

    eISSN： 1880-3865

  24. Profiling of the microbiota in the remaining sports drink and orange juice in plastic bottles after direct drinking. International-journal Peer-reviewed

    Miho Kawachi, Anna Wakui, Nagara Kaku, Nanase Takahashi, Shingo Maruyama, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of Oral Biosciences　64　(4)　437-444　2022/08/22

    DOI： 10.1016/j.job.2022.08.003 　

    ISSN： 1349-0079

    eISSN： 1880-3865

  25. Development and evaluation of a chewing gum containing antimicrobial peptide GH12 for caries prevention. International-journal Peer-reviewed

    Xuelian Jiang, Yufei Wang, Xinwei Li, Zening Feng, Yuhao Zeng, Sili Han, Nobuhiro Takahashi, Linglin Zhang

    European journal of oral sciences　130　(5)　e12887　2022/08/02

    DOI： 10.1111/eos.12887 　

    ISSN： 0909-8836

    eISSN： 1600-0722

  26. Microbiota profiles on the surface of non-woven fabric masks after wearing Peer-reviewed

    Shingo Maruyama, Hiroto Sano, Anna Wakui, Miho Kawachi, Nagara Kaku, Nanase Takahashi, Misato Miyazawa, Takashi Abe, Aya Sato, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of Oral Biosciences　64　(3)　376-379　2022/08

    Publisher: Elsevier BV

    DOI： 10.1016/j.job.2022.07.002 　

    ISSN： 1349-0079

    eISSN： 1880-3865

  27. Oral Microbiome Profiles and Inflammation in Pregnant Women Who Used Orthodontic Appliances. International-journal Peer-reviewed

    Fajar Kusuma Dwi Kurniawan, Retno Indrawati Roestamadji, Nobuhiro Takahashi, Udijanto Tedjosasongko, Ida Bagus Narmada, Meircurius Dwi Condro Surboyo, Indeswati Diyatri

    Dentistry journal　10　(7)　118　2022/07/01

    DOI： 10.3390/dj10070118 　

    eISSN： 2304-6767

  28. Influence of alcohol sensitivity on bone metastases and skeletal-related events in primary operable breast cancer: A retrospective cohort study Peer-reviewed

    Naoko Tanda, Hiroshi Tada, Jumpei Washio, Nobuhiro Takahashi, Takanori Ishida, Takeyoshi Koseki

    PLOS ONE　17　(6)　e0269335-e0269335　2022/06/03

    Publisher: Public Library of Science (PLoS)

    DOI： 10.1371/journal.pone.0269335 　

    eISSN： 1932-6203

  29. Effectiveness of an actuator-driven pulsed water jet for removal of softened carious dentin. Peer-reviewed

    Yuka Yokoyama-Sato, Takashi Nishioka, Yukihiro Naganuma, Masatoshi Takahashi, Atsuhiro Nakagawa, Nobuhiro Yoda, Keiichi Sasaki, Nobuhiro Takahashi, Teiji Tominaga, Masahiro Iikubo

    Dental materials journal　41　(4)　527-533　2022/03/10

    DOI： 10.4012/dmj.2021-234 　

    ISSN： 0287-4547

    eISSN： 1881-1361

  30. The Importance of Nitrate Reduction for Oral Health. International-journal Peer-reviewed

    B T Rosier, N Takahashi, E Zaura, B P Krom, R M MartÍnez-Espinosa, S G J van Breda, P D Marsh, A Mira

    Journal of dental research　101　(8)　887-897　2022/02/23

    DOI： 10.1177/00220345221080982 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  31. Rewired Cellular Metabolic Profiles in Response to Metformin under Different Oxygen and Nutrient Conditions. International-journal Peer-reviewed

    Shan Liu, Jumpei Washio, Satoko Sato, Yuki Abiko, Yuta Shinohara, Yuri Kobayashi, Haruki Otani, Shiori Sasaki, Xiaoyi Wang, Nobuhiro Takahashi

    International journal of molecular sciences　23　(2)　2022/01/17

    DOI： 10.3390/ijms23020989 　

    ISSN： 1661-6596

    eISSN： 1422-0067

  32. Facile Fabrication of All-solid-state Ion-selective Electrodes by Laminating and Drop-casting for Multi-sensing Peer-reviewed

    Shingo Okubo, Yoshihisa Ozeki, Tetsuya Yamada, Kosuke Saito, Noboru Ishihara, Yasuko Yanagida, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi

    Electrochemistry　90　(7)　2022

    DOI： 10.5796/ELECTROCHEMISTRY.22-00020 　

    ISSN： 1344-3542

    eISSN： 2186-2451

  33. A detection and analysis of Fusobacterium utilizing changes in the magnetic properties of magnetic nanoparticlesantibody-antigen aggregates Peer-reviewed

    S. Yabukami, T. Murayama, S. Takahashi, S. Ohno, J. Washio, N. Takahashi

    IEEE Transactions on Magnetics　58　(81)　5300306-1　2022

    Publisher: Institute of Electrical and Electronics Engineers (IEEE)

    DOI： 10.1109/tmag.2022.3161508 　

    ISSN： 0018-9464

    eISSN： 1941-0069

  34. A water-soluble glass-based temporary restorative resin inhibited bacteria-induced pH reductions at the bacteria-material interface. Peer-reviewed

    Gen Mayanagi, Luo Yufang, Tomohiro Hoshino, Nobuhiro Takahashi

    Dental materials journal　41　(1)　95-100　2021/09/04

    DOI： 10.4012/dmj.2021-129 　

    ISSN： 0287-4547

    eISSN： 1881-1361

  35. Profiling system of oral microbiota utilizing polymerase chain reaction-restriction fragment length polymorphism analysis. International-journal Peer-reviewed

    Hiroto Sano, Anna Wakui, Miho Kawachi, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of oral biosciences　63　(3)　292-297　2021/06/07

    DOI： 10.1016/j.job.2021.05.003 　

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    OBJECTIVES: Profiling of oral microbiota has traditionally been performed using conventional methods. These methods are relatively time-consuming and labor-intensive. Metagenomic analysis of oral microbiota using high-speed next-generation sequencing is a highly promising technology. However, it is expensive. This study sought to develop a simple and cost-effective profiling method for oral microbiota using 16S rRNA gene polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of PCR-amplified 16S ribosomal RNA genes. METHODS: Oral isolates of 59 bacterial species from human saliva, including Streptococcus, Actinomyces, and Veillonella, were cultured anaerobically on CDC Anaerobe 5% sheep blood agar plates. Genomic DNA was extracted from single colonies and 16S rRNA genes were PCR-amplified using the 27F and 1492R universal primers. The PCR products were purified and characterized by single digestion with HpaII restriction endonuclease. 16S rRNA gene sequences were obtained from the GenBank database, and the expected restriction profiles were compared with the RFLP patterns obtained from agarose gel electrophoresis. RESULTS: Sixty-five RFLP patterns were obtained from 27 genera and 59 species. The expected fragment sizes of these species were calculated based on GenBank 16S rRNA gene sequences. Fifty-nine patterns were obtained from the analysis of GenBank sequences. The RFLP patterns produced with HpaII distinguished many oral bacterial species. RFLP patterns enabling identification of oral bacteria were generated. The 16S rRNA gene PCR-RFLP analysis did not require expensive equipment and reagents and was cost-effective. CONCLUSION: PCR-RFLP analysis based on 16S rRNA genes could be an alternative method for oral microbiota analysis in smaller laboratories.

  36. Effectiveness and safety of a new dental plaque removal device utilizing micro mist spray for removing oral biofilm in vitro. International-journal Peer-reviewed

    Hiroki Hihara, Ryo Tagaino, Jumpei Washio, Kittipong Laosuwan, Dimas Prasetianto Wicaksono, Kuniyuki Izumita, Rie Koide, Nobuhiro Takahashi, Keiichi Sasaki

    BMC oral health　21　(1)　286-286　2021/06/04

    DOI： 10.1186/s12903-021-01647-4 　

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    BACKGROUND: Removal of oral biofilm from the oral mucosa is essential for preventing risk of respiratory and gastrointestinal infection in elderly people. Currently, no device is available which can remove oral biofilm from oral mucosa effectively and safely. Therefore, the effectiveness and safety of the Micro Scale Mist UNIT (MSM-UNIT), a newly developed dental plaque removal device utilizing high speed sprays of fine water droplets, were evaluated for biofilm removal, including the rate and surface roughness for simulated tooth surface and mucous membrane. METHODS: Simulated tooth and oral mucosa coated with an artificial biofilm of Streptococcus mutans were used for evaluation of effectiveness, with uncoated substrates as the controls. The MSM-UNIT and a conventional air ablation device were operated under recommended instructions. The effectiveness was evaluated from the rate of removal of the biofilm, and the safety was evaluated from the damage observed by scanning electron microscope and surface roughness. RESULTS: The biofilm removal rate of the MSM-UNIT was significantly higher than that of AIRFLOW. Little damage was observed in the area treated by the MSM-UNIT. The surface roughness of the MSM-UNIT treated area on simulated tooth surface and oral mucosa showed no significant difference to the control area. In contrast, cracks and powder were observed in the area treated by AIRFLOW. In particular, the surface roughness of the AIRFLOW treated area for Toughsilon was significantly larger than that of the control. CONCLUSIONS: The MSM-UNIT could be used safely and effectively for removing biofilm not only on simulated tooth surfaces but also simulated mucous membrane. The MSM-UNIT has no harmful effect on teeth or oral mucosa, and may be used for comprehensive oral care for patients during nursing care and the perioperative period.

  37. Bacterial concentration and composition in liquid baby formula and a baby drink consumed with an artificial nipple. International-journal Peer-reviewed

    Anna Wakui, Hiroto Sano, Miho Kawachi, Ayaka Aida, Yuta Takenaka, Akane Yonezawa, Nana Nakahata, Sachie Moriyama, Mayumi Nishikata, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Reiko Sakashita, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of oral biosciences　63　(2)　161-168　2021/06

    DOI： 10.1016/j.job.2021.03.001 　

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    OBJECTIVES: To clarify the characteristics and growth of bacteria that may infiltrate liquid baby formula during feeding and after storage for more than 3 h, the transfer of oral bacteria through artificial nipples, and bacterial survival in liquid baby formula and a baby drink were examined immediately after drinking and after storage at 4 °C for 12 h and 24 h. METHODS: Thirteen human subjects (aged 19-24 years) were asked to drink approximately 50 mL of liquid baby formula and a baby drink, via the artificial nipple of a baby bottle. Samples of the remaining liquid after storage at 4 °C for 12 h and 24 h were inoculated onto blood agar plates and incubated anaerobically at 37 °C for 7 days. Genomic DNA was extracted from individual colonies, and the bacterial species were identified by 16S rRNA gene sequencing. RESULTS: The mean concentrations of bacteria in the liquid baby formula were (2.6 ± 2.8) × 104 and (4.1 ± 6.6) × 104 colony-forming unit/mL after storage at 4 °C for 12 h and 24 h, respectively. Streptococcus (43.2%), Veillonella (9.3%), and Schaalia (8.2%) species were recovered from the remaining liquid baby formula after storage at 4 °C for 12 h. In contrast, no bacteria were detected in the remaining baby drink after storage at 37 °C for 24 h. CONCLUSIONS: The levels of bacteria immediately after drinking and after storage at 4 °C for 12 h or 24 h were similar, suggesting that remaining liquid baby formula may be preserved safely in a refrigerator for more than 3 h.

  38. Profiling of Microbiota at the Mouth of Bottles and in Remaining Tea after Drinking Directly from Plastic Bottles of Tea. International-journal Peer-reviewed

    Anna Wakui, Hiroto Sano, Yuka Hirabuki, Miho Kawachi, Ayaka Aida, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Dentistry journal　9　(6)　58　2021/05/21

    DOI： 10.3390/dj9060058 　

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    It has been speculated that oral bacteria can be transferred to tea in plastic bottles when it is drunk directly from the bottles, and that the bacteria can then multiply in the bottles. The transfer of oral bacteria to the mouth of bottles and bacterial survival in the remaining tea after drinking directly from bottles were examined immediately after drinking and after storage at 37 °C for 24 h. Twelve healthy subjects (19 to 23 years of age) were asked to drink approximately 50 mL of unsweetened tea from a plastic bottle. The mouths of the bottles were swabbed with sterile cotton, and the swabs and the remaining tea in the bottles were analyzed by anaerobic culture and 16S rRNA gene sequencing. Metagenomic analysis of the 16S rRNA gene was also performed. The mean amounts of bacteria were (1.8 ± 1.7) × 104 colony-forming units (CFU)/mL and (1.4 ± 1.5) × 104 CFU/mL at the mouth of the bottles immediately after and 24 h after drinking, respectively. In contrast, (0.8 ± 1.6) × 104 CFU/mL and (2.5 ± 2.6) × 106 CFU/mL were recovered from the remaining tea immediately after and 24 h after drinking, respectively. Streptococcus (59.9%) were predominant at the mouth of the bottles immediately after drinking, followed by Schaalia (5.5%), Gemella (5.5%), Actinomyces (4.9%), Cutibacterium (4.9%), and Veillonella (3.6%); the culture and metagenomic analyses showed similar findings for the major species of detected bacteria, including Streptococcus (59.9%, and 10.711%), Neisseria (1.6%, and 24.245%), Haemophilus (0.6%, and 15.658%), Gemella (5.5%, and 0.381%), Cutibacterium (4.9%, and 0.041%), Rothia (2.6%, and 4.170%), Veillonella (3.6%, and 1.130%), Actinomyces (4.9%, and 0.406%), Prevotella (1.6%, and 0.442%), Fusobacterium (1.0%, and 0.461%), Capnocytophaga (0.3%, and 0.028%), and Porphyromonas (1.0%, and 0.060%), respectively. Furthermore, Streptococcus were the most commonly detected bacteria 24 h after drinking. These findings demonstrated that oral bacteria were present at the mouth of the bottles and in the remaining tea after drinking.

  39. Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions. International-journal Peer-reviewed

    Yuta Shinohara, Jumpei Washio, Yuri Kobayashi, Yuki Abiko, Keiichi Sasaki, Nobuhiro Takahashi

    PloS one　16　(10)　e0254966　2021

    DOI： 10.1371/journal.pone.0254966 　

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    OBJECTIVE: The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore, we investigated the effects of oxygen concentration fluctuations on the glucose metabolism of cancer cells. METHODS: The glucose metabolism of oral squamous cell carcinoma (HSC-2 and HSC-3) and normal epithelial (HaCaT) cells cultured under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions was measured using a pH-stat system under normoxic or hypoxic conditions. The acidic end-products and reactive oxygen species (ROS) generated by glucose metabolism were also measured. RESULTS: Under normoxic conditions, the metabolic activity of hypoxically cultured cancer cells was significantly increased, and the production of acids other than lactate was upregulated, while the normal cells did not respond to rapid increases in oxygen levels. ROS production was higher in normoxic conditions in all cells, especially the hypoxically cultured HSC-3 cells. CONCLUSIONS: Rapid increases in oxygen levels might enhance the glucose metabolism of hypoxically cultured cancer cells by mainly activating the TCA cycle and electron transport system, which might activate cancer cells through the ATP and ROS generation.

  40. Green Tea-Derived Epigallocatechin Gallate Inhibits Acid Production and Promotes the Aggregation of Streptococcus mutans and Non-Mutans Streptococci. International-journal Peer-reviewed

    Sili Han, Yuki Abiko, Jumpei Washio, Yufang Luo, Linglin Zhang, Nobuhiro Takahashi

    Caries research　55　(3)　205-214　2021

    DOI： 10.1159/000515814 　

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    It has been suggested that green tea-derived epigallocatechin gallate (EGCG), which has antimicrobial properties, might help prevent dental caries. However, the detailed properties of EGCG remain unclear. In this study, the antimicrobial properties of EGCG were evaluated by examining its bactericidal activity, its inhibitory effects against bacterial growth, acid production, acidic end-product formation, and sugar uptake (phosphoenolpyruvate-dependent phosphotransferase system, PEP-PTS activity), and its effects on bacterial aggregation, using monocultured planktonic cells of Streptococcus mutans and non-mutans streptococci. Coincubating S. mutans with EGCG (1 mg/mL) for 4 h had no bactericidal effects, while it decreased the growth and acid production of S. mutans by inhibiting the activity of the PEP-PTS. EGCG (2 mg/mL) caused rapid bacterial cell aggregation and had reduced the optical density of S. mutans cell suspension by 86.7% at pH 7.0 and 90.7% at pH 5.5 after 2 h. EGCG also reduced the acid production of non-mutans streptococci, including S. sanguinis, S. gordonii, and S. salivarius, and promoted the aggregation of these non-mutans streptococci. Furthermore, these antimicrobial effects of short-term EGCG treatment persisted in the presence of saliva. These results suggest that EGCG might have short-term antibacterial effects on caries-associated streptococci in the oral cavity.

  41. Nitrite-producing oral microbiome in adults and children International-journal Peer-reviewed

    Yuria Sato-Suzuki, Jumpei Washio, Dimas Prasetianto Wicaksono, Takuichi Sato, Satoshi Fukumoto, Nobuhiro Takahashi

    Scientific Reports　10　(1)　16652-16652　2020/12/01

    DOI： 10.1038/s41598-020-73479-1 　

    eISSN： 2045-2322

  42. Nitrite Production from Nitrate and Its Link with Lactate Metabolism in Oral Veillonella spp. International-journal Peer-reviewed

    Dimas Prasetianto Wicaksono, Jumpei Washio, Yuki Abiko, Hitomi Domon, Nobuhiro Takahashi

    Applied and Environmental Microbiology　86　(20)　01255-20　2020/08/07

    Publisher: American Society for Microbiology

    DOI： 10.1128/aem.01255-20 　

    ISSN： 0099-2240

    eISSN： 1098-5336

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    <title>ABSTRACT</title> <italic>Veillonella</italic> species are among the major anaerobes in the oral cavity and are frequently detected in both caries lesions and healthy oral microbiomes. They possess the ability to utilize lactate and convert nitrate (NO3–) into nitrite (NO2–). Recently, interest in NO2– has increased rapidly because of its beneficial effects on oral and general health; i.e., it inhibits the growth and metabolism of oral pathogenic bacteria, such as <named-content content-type="genus-species">Streptococcus mutans</named-content>, and lowers systemic blood pressure. However, there is only limited information about the biochemical characteristics of NO2– production by <italic>Veillonella</italic> species. We found that NO3– did not inhibit the growth of <named-content content-type="genus-species">Veillonella atypica</named-content> or <named-content content-type="genus-species">Veillonella parvula</named-content>, and it inhibited the growth of <named-content content-type="genus-species">Streptococcus mutans</named-content> only at a high concentration (100 mM). However, NO2– inhibited the growth of <named-content content-type="genus-species">Streptococcus mutans</named-content> at a low concentration (0.5 mM), while a higher concentration of NO2– (20 mM) was needed to inhibit the growth of <italic>Veillonella</italic> species. NO2– production by <italic>Veillonella</italic> species was increased by environmental factors (lactate, acidic pH, and anaerobic conditions) and growth conditions (the presence of NO3– or NO2–) and was linked to anaerobic lactate metabolism. A stoichiometric evaluation revealed that NO3– is reduced to NO2– by accepting reducing power derived from the oxidization of lactate. These findings suggest that the biochemical characteristics of NO2– production from NO3– and its linkage with lactate metabolism in oral <italic>Veillonella</italic> species may play a key role in maintaining good oral and general health. <bold>IMPORTANCE</bold> The prevalence of dental caries is still high around the world. Dental caries is initiated when the teeth are exposed to acid, such as lactic acid, produced via carbohydrate metabolism by acidogenic microorganisms. <italic>Veillonella</italic> species, which are among the major oral microorganisms, are considered to be beneficial bacteria due to their ability to convert lactic acid to weaker acids and to produce NO2– from NO3–, which is thought to be good for both oral and general health. Therefore, it is clear that there is a need to elucidate the biochemical characteristics of NO2– production in <italic>Veillonella</italic> species. The significance of our research is that we have found that lactate metabolism is linked to NO2– production by <italic>Veillonella</italic> species in the environment found in the oral cavity. This study suggests that <italic>Veillonella</italic> species are potential candidates for maintaining oral and general health.

  43. Electrochemical evaluation of the hydrogen peroxide- and fluoride-induced corrosive property and its recovery on the titanium surface Peer-reviewed

    Satoko Furiya-Sato, Azusa Fukushima, Gen Mayanagi, Keiichi Sasaki, Nobuhiro Takahashi

    Journal of Prosthodontic Research　64　(3)　307-312　2020/07

    DOI： 10.1016/j.jpor.2019.09.002 　

    ISSN： 1883-1958

  44. Sugar Metabolism of Scardovia wiggsiae, a Novel Caries-Associated Bacterium Peer-reviewed

    Mai Kameda, Yuki Abiko, Jumpei Washio, Anne C.R. Tanner, Christine A. Kressirer, Itaru Mizoguchi, Nobuhiro Takahashi

    Frontiers in Microbiology　11　479　2020/03/25

    DOI： 10.3389/fmicb.2020.00479 　

    eISSN： 1664-302X

  45. Integrated hypothesis of dental caries and periodontal diseases Peer-reviewed

    Bente Nyvad, Nobuhiro Takahashi

    Journal of Oral Microbiology　12　(1)　1710953　2020/01/01

    DOI： 10.1080/20002297.2019.1710953 　

    eISSN： 2000-2297

  46. Use of cellulose nanofibers as a denture immersing solution Peer-reviewed

    Wei Zou, Guang Hong, Yukiko Yamazaki, Kazuma Takase, Toru Ogawa, Jumpei Washio, Nobuhiro Takahashi, Keiichi Sasaki

    Dental Materials Journal　39　(1)　80-88　2020

    DOI： 10.4012/dmj.2018-388 　

    ISSN： 0287-4547

    eISSN： 1881-1361

  47. Metabolic property of acetaldehyde production from ethanol and glucose by oral Streptococcus and Neisseria Peer-reviewed

    Ryo Tagaino, Jumpei Washio, Yuki Abiko, Naoko Tanda, Keiichi Sasaki, Nobuhiro Takahashi

    Scientific Reports　9　(1)　10446　2019/12/01

    DOI： 10.1038/s41598-019-46790-9 　

    eISSN： 2045-2322

  48. Draft genome sequences of two veillonella tobetsuensis clinical isolates from intraoperative bronchial fluids of elderly patients with pulmonary carcinoma Peer-reviewed

    Izumi Mashima, Tohru Miyoshi-Akiyama, Junko Tomida, Ryo Kutsuna, Jumpei Washio, Nobuhiro Takahashi, Futoshi Nakazawa, Takuichi Sato, Yoshiaki Kawamura

    Microbiology Resource Announcements　8　(38)　e00397-19　2019/09/19

    DOI： 10.1128/MRA.00397-19 　

    eISSN： 2576-098X

  49. Sodium fluoride and silver diamine fluoride-coated tooth surfaces inhibit bacterial acid production at the bacteria/tooth interface Peer-reviewed

    Tomoko Ishiguro, Gen Mayanagi, Marika Azumi, Haruki Otani, Azusa Fukushima, Keiichi Sasaki, Nobuhiro Takahashi

    Journal of Dentistry　84　30-35　2019/05

    DOI： 10.1016/j.jdent.2018.12.017 　

    ISSN： 0300-5712

  50. フッ化物応用をめぐる誤解を解く 生化学的吸収メカニズムを再確認し、フッ化水素を理解する

    高橋 信博

    口腔衛生学会雑誌　69　(増刊)　111-112　2019/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  51. Professional oral care reduces carcinogenic acetaldehyde levels in mouth air of perioperative esophageal cancer patients: A prospective comparative study Peer-reviewed

    Naoko Tanda, Jumpei Washio, Takashi Kamei, Kohei Akazawa, Nobuhiro Takahashi, Takeyoshi Koseki

    Tohoku Journal of Experimental Medicine　249　(1)　75-83　2019

    DOI： 10.1620/tjem.249.75 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  52. Profiling of microbiota in liquid baby formula consumed with an artificial nipple Peer-reviewed

    Hiroto Sano, Anna Wakui, Miho Kawachi, Rito Kato, Sachie Moriyama, Mayumi Nishikata, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Reiko Sakashita, Junko Tomida, Yoshiaki Kawamura, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Biomedical Research (Japan)　40　(4)　163-168　2019

    DOI： 10.2220/biomedres.40.163 　

    ISSN： 0388-6107

    eISSN： 1880-313X

  53. Acidogenic potential of oral bifidobacterium and its high fluoride tolerance Peer-reviewed

    Ayumi Manome, Yuki Abiko, Junko Kawashima, Jumpei Washio, Satoshi Fukumoto, Nobuhiro Takahashi

    Frontiers in Microbiology　10　(MAY)　1099　2019

    DOI： 10.3389/fmicb.2019.01099 　

    eISSN： 1664-302X

  54. Exhaled acetone and isoprene in perioperative lung cancer patients under intensive oral care: Possible indicators of inflammatory responses a nd metabolic changes Peer-reviewed

    Naoko Tanda, Yasushi Hoshikawa, Takuichi Sato, Nobuhiro Takahashi, Takeyoshi Koseki

    Biomedical Research (Japan)　40　(1)　29-36　2019

    DOI： 10.2220/biomedres.40.29 　

    ISSN： 0388-6107

    eISSN： 1880-313X

  55. Inhibitory effect of resin composite containing S-PRG filler on Streptococcus mutans glucose metabolism. International-journal Peer-reviewed

    Haruaki Kitagawa, Saeki Miki-Oka, Gen Mayanagi, Yuki Abiko, Nobuhiro Takahashi, Satoshi Imazato

    Journal of dentistry　70　92-96　2018/03

    DOI： 10.1016/j.jdent.2017.12.017 　

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    OBJECTIVES: Resin composites containing surface pre-reacted glass-ionomer (S-PRG) fillers have been reported to inhibit Streptococcus mutans growth on their surfaces, and their inhibitory effects were attributed to BO33- and F- ions. The aim of this study was to evaluate S. mutans acid production through glucose metabolism on resin composite containing S-PRG fillers and assess inhibitory effects of BO33- and F- on S. mutans metabolic activities. METHODS: The pH change through S. mutans acid production on experimental resin composite was periodically measured after the addition of glucose. Inhibitory effects of BO33- or F- solutions on S. mutans metabolism were evaluated by XTT assays and measurement of the acid production rate. RESULTS: The pH of experimental resin containing S-PRG fillers was significantly higher than that of control resin containing silica fillers (p < 0.05). OD450 values by XTT assays and S. mutans acid production rates significantly decreased in the presence of BO33- and F- compared with the absence of these ions (p < 0.05). CONCLUSIONS: pH reduction by S. mutans acid production was inhibited on resin composite containing S-PRG fillers. Moreover, S. mutans glucose metabolism and acid production were inhibited in the presence of low concentrations of BO33- or F-. CLINICAL SIGNIFICANCE: BO33- or F- released from resin composite containing S-PRG fillers exhibits inhibitory effects on S. mutans metabolism at concentrations lower than those which inhibit bacterial growth.

  56. 歯科用ユニットの水質管理－“フラッシング”の重要性－ Invited Peer-reviewed

    山田将博, 鷲尾純平, 高橋信博, 江草 宏

    日本歯科理工学会誌　37　(4)　223-226　2018

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  57. Microbiological deterioration of dental materials - A consideration of biochemical mechanisms behind bacterial metabolic activities - Peer-reviewed

    Nobuhiro Takahashi

    Funtai Oyobi Fummatsu Yakin/Journal of the Japan Society of Powder and Powder Metallurgy　65　489-494　2018/01

    DOI： 10.2497/jjspm.65.489 　

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    Since the oral cavity is open to the external environment, a tremendous number of microorganisms adhere to its surface and form a biofilm on it. The microorganisms in the oral biofilm cohabit with the host as the indigenous microbiota and utilize nutrients, such as carbohydrates, proteins, and amino acids, which are supplied from bodily fluid (saliva, etc.) and dietary foods. However, when the balance between the host and the bacteria is disrupted by changes in the host&#039;s eating habits, prophylactic behavior, immunological activity, etc., oral diseases can occur. The excessive and frequent intake of carbohydrates might increase bacterial acid production from carbohydrates, initiating dental caries, while poor oral hygiene might cause bacteria to accumulate and promote the bacterial production of cytotoxic metabolites from proteins and amino acids, initiating periodontal diseases. When dental biomaterials are placed in the oral cavity, their surfaces also become covered by the oral biofilm and are influenced by bacterial metabolism. Our recent studies suggested that bacteria can invade polymethyl methacrylate and reduce its hardness and alter its components, and bacterial oxygen consumption can create oxygen concentration cells on titanium surfaces and cause then to corrode. Possible mechanisms that might underpin these microbiological deteriorations were discussed.

  58. Corrosive effects of fluoride on titanium under artificial biofilm Peer-reviewed

    Azusa Fukushima, Gen Mayanagi, Keiichi Sasaki, Nobuhiro Takahashi

    Journal of Prosthodontic Research　62　(1)　104-109　2018/01/01

    Publisher: Elsevier Ltd

    DOI： 10.1016/j.jpor.2017.08.004 　

    ISSN： 1883-1958

  59. Real-time monitoring system for evaluating the acid-producing activity of oral squamous cell carcinoma cells at different environmental pH Peer-reviewed

    Hiromitsu Morishima, Jumpei Washio, Jun Kitamura, Yuta Shinohara, Tetsu Takahashi, Nobuhiro Takahashi

    SCIENTIFIC REPORTS　7　1092　2017/08

    DOI： 10.1038/s41598-017-10893-y 　

    ISSN： 2045-2322

  60. 連携機能を活用した歯学教育高度化プログラム

    竹石 龍右, 加来 咲子, 天雲 太一, 冨士 岳志, 三村 純代, 首藤 崇裕, 石田 陽子, 秋葉 奈美, 魚島 勝美, 小野 和宏, 高橋 信博, 二川 浩樹, 加藤 功一, 佐々木 啓一, 前田 健康

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　36回　125-125　2017/07

    Publisher: (一社)日本歯科医学教育学会

  61. Second Era of OMICS in Caries Research: Moving Past the Phase of Disillusionment Peer-reviewed

    M. M. Nascimento, E. Zaura, A. Mira, N. Takahashi, J. M. ten Cate

    JOURNAL OF DENTAL RESEARCH　96　(7)　733-740　2017/07

    DOI： 10.1177/0022034517701902 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  62. Inhibitory Effects of Nitrite on Acid Production in Dental Plaque in Children Peer-reviewed

    Yuji Yamamoto, Jumpei Washio, Koichi Shimizu, Koei Igarashi, Nobuhiro Takahashi

    ORAL HEALTH & PREVENTIVE DENTISTRY　15　(2)　153-156　2017/04

    DOI： 10.3290/j.ohpd.a37926 　

    ISSN： 1602-1622

    eISSN： 1757-9996

  63. pH Response and Tooth Surface Solubility at the Tooth/Bacteria Interface Peer-reviewed

    Gen Mayanagi, Koei Igarashi, Jumpei Washio, Nobuhiro Takahashi

    CARIES RESEARCH　51　(2)　160-166　2017

    DOI： 10.1159/000454781 　

    ISSN： 0008-6568

    eISSN： 1421-976X

  64. Formation of bioactive N-doped TiO2 on Ti with visible light-induced antibacterial activity using NaOH, hot water, and subsequent ammonia atmospheric heat treatment Peer-reviewed

    Masakazu Kawashita, Naoko Endo, Tomoaki Watanabe, Toshiki Miyazaki, Maiko Furuya, Kotoe Yokota, Yuki Abiko, Hiroyasu Kanetaka, Nobuhiro Takahashi

    COLLOIDS AND SURFACES B-BIOINTERFACES　145　285-290　2016/09

    DOI： 10.1016/j.colsurfb.2016.05.017 　

    ISSN： 0927-7765

    eISSN： 1873-4367

  65. Metabolomic studies of oral biofilm, oral cancer, and beyond Peer-reviewed

    Jumpei Washio, Nobuhiro Takahashi

    International Journal of Molecular Sciences　17　(6)　2016/06/01

    DOI： 10.3390/ijms17060870 　

    ISSN： 1422-0067 1661-6596

  66. Profiling subgingival microbiota of plaque biofilms in the elderly Peer-reviewed

    Yuki Abiko, Takuichi Sato, Reiko Sakashita, Junko Tomida, Yoshiaki Kawamura, Nobuhiro Takahashi

    Journal of Oral Biosciences　58　(2)　62-65　2016/05/01

    Publisher: Japanese Association for Oral Biology

    DOI： 10.1016/j.job.2015.12.002 　

    ISSN： 1349-0079

  67. Amino acid composition and amino acid-metabolic network in supragingival plaque Peer-reviewed

    Jumpei Washio, Tamaki Ogawa, Keisuke Suzuki, Yosuke Tsukiboshi, Motohiro Watanabe, Nobuhiro Takahashi

    BIOMEDICAL RESEARCH-TOKYO　37　(4)　251-257　2016

    DOI： 10.2220/biomedres.37.251 　

    ISSN： 0388-6107

    eISSN： 1880-313X

  68. PCR-dipstick DNA chromatography for profiling of a subgroup of caries-associated bacterial species in plaque from healthy coronal surfaces and periodontal pockets

    Lingyang Tian, Takuichi Sato, Kousuke Niwa, Mitsuo Kawase, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi

    BIOMEDICAL RESEARCH-TOKYO　37　(1)　29-36　2016

    DOI： 10.2220/biomedres.37.29 　

    ISSN： 0388-6107

    eISSN： 1880-313X

  69. Ecological Hypothesis of Dentin and Root Caries

    Nobuhiro Takahashi, Bente Nyvad

    CARIES RESEARCH　50　(4)　422-431　2016

    DOI： 10.1159/000447309 　

    ISSN： 0008-6568

    eISSN： 1421-976X

  70. Oral Microbiome Metabolism: From "Who Are They?" to "What Are They Doing?"

    N. Takahashi

    JOURNAL OF DENTAL RESEARCH　94　(12)　1628-1637　2015/12

    DOI： 10.1177/0022034515606045 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  71. Nitrogenous compounds stimulate glucose-derived acid production by oral Streptococcus and Actinomyces

    Yuka Norimatsu, Junko Kawashima, Teruko Takano-Yamamoto, Nobuhiro Takahashi

    MICROBIOLOGY AND IMMUNOLOGY　59　(9)　501-506　2015/09

    DOI： 10.1111/1348-0421.12283 　

    ISSN： 0385-5600

    eISSN： 1348-0421

  72. Porphyromonas bronchialis sp nov Isolated from Intraoperative Bronchial Fluids of a Patient with Non-Small Cell Lung Cancer

    Takuichi Sato, Junko Tomida, Takashi Naka, Nagatoshi Fujiwara, Ayako Hasegawa, Yasushi Hoshikawa, Junko Matsuyama, Naoko Ishida, Takashi Kondo, Kaori Tanaka, Nobuhiro Takahashi, Yoshiaki Kawamura

    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE　237　(1)　31-37　2015/09

    DOI： 10.1620/tjem.237.31 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  73. Real-time monitoring of the metabolic activity of periodontopathic bacteria

    Kazuko Ishiguro, Jumpei Washio, Keiichi Sasaki, Nobuhiro Takahashi

    JOURNAL OF MICROBIOLOGICAL METHODS　115　22-26　2015/08

    DOI： 10.1016/j.mimet.2015.05.015 　

    ISSN： 0167-7012

    eISSN： 1872-8359

  74. ラット大臼歯における感染歯髄のαTCP含有抗生物質に対する応答(Responses of infected dental pulp to αTCP-containing antimicrobials in rat molars)

    Sato Takuichi, Kenmotsu Shin-ichi, Nakakura-Ohshima Kuniko, Takahashi Nobuhiro, Ohshima Hayato

    Archives of Histology and Cytology　73　(4-5)　165-175　2015/07

    Publisher: 国際組織細胞学会

    ISSN： 0914-9465

  75. Microbiota profiling of bronchial fluids of elderly patients with pulmonary carcinoma

    Naoko Ishida, Takuichi Sato, Yasushi Hoshikawa, Naoko Tanda, Keiichi Sasaki, Takashi Kondo, Nobuhiro Takahashi

    Journal of Oral Biosciences　57　(2)　110-117　2015/05/01

    Publisher: Japanese Association for Oral Biology

    DOI： 10.1016/j.job.2014.11.001 　

    ISSN： 1349-0079

  76. Deterioration of polymethyl methacrylate dentures in the oral cavity

    Hiroshi Matsuo, Hanako Suenaga, Masatoshi Takahashi, Osamu Suzuki, Keiichi Sasaki, Nobuhiro Takahashi

    DENTAL MATERIALS JOURNAL　34　(2)　234-239　2015/04

    DOI： 10.4012/dmj.2014-089 　

    ISSN： 0287-4547

  77. Oral malodorous gases and oral microbiota: From halitosis to carcinogenesis

    Naoko Tanda, Yasushi Hoshikawa, Naoko Ishida, Takuichi Sato, Nobuhiro Takahashi, Ryoichi Hosokawa, Takeyoshi Koseki

    Journal of Oral Biosciences　57　(4)　175-178　2015

    Publisher: Japanese Association for Oral Biology

    DOI： 10.1016/j.job.2015.05.004 　

    ISSN： 1349-0079

  78. Analysis of ketone bodies in exhaled breath and blood of ten healthy Japanese at OGTT using a portable gas chromatograph

    Naoko Tanda, Yoshinori Hinokio, Jumpei Washio, Nobuhiro Takahashi, Takeyoshi Koseki

    JOURNAL OF BREATH RESEARCH　8　(4)　2014/12

    DOI： 10.1088/1752-7155/8/4/046008 　

    ISSN： 1752-7155

    eISSN： 1752-7163

  79. Glucose and glutamine metabolism in oral squamous cell carcinoma: insight from a quantitative metabolomic approach

    Tamaki Ogawa, Jumpei Washio, Tetsu Takahashi, Seishi Echigo, Nobuhiro Takahashi

    ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY　118　(2)　218-225　2014/08

    DOI： 10.1016/j.oooo.2014.04.003 　

    ISSN： 2212-4403

    eISSN： 1528-395X

  80. Detection and identification of oral anaerobes in intraoperative bronchial fluids of patients with pulmonary carcinoma

    Ayako Hasegawa, Takuichi Sato, Yasushi Hoshikawa, Naoko Ishida, Naoko Tanda, Yoshiaki Kawamura, Takashi Kondo, Nobuhiro Takahashi

    MICROBIOLOGY AND IMMUNOLOGY　58　(7)　375-381　2014/07

    DOI： 10.1111/1348-0421.12157 　

    ISSN： 0385-5600

    eISSN： 1348-0421

  81. Effects of pH and Lactate on Hydrogen Sulfide Production by Oral Veillonella spp.

    Jumpei Washio, Yuko Shimada, Masakazu Yamada, Ryouichi Sakamaki, Nobuhiro Takahashi

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY　80　(14)　4184-4188　2014/07

    DOI： 10.1128/AEM.00606-14 　

    ISSN： 0099-2240

    eISSN： 1098-5336

  82. 口腔生態系から見た口臭 生化学的、生態学的アプローチ

    高橋 信博

    日本口臭学会会誌　5　(1)　3-7　2014/06

    Publisher: 日本口臭学会

    ISSN： 1884-4901

  83. Inhibitory effect of Ti-Ag alloy on artificial biofilm formation

    Kazuko Nakajo, Masatoshi Takahashi, Masafumi Kikuchi, Yukyo Takada, Osamu Okuno, Keiichi Sasaki, Nobuhiro Takahashi

    DENTAL MATERIALS JOURNAL　33　(3)　389-393　2014/06

    DOI： 10.4012/dmj.2013-334 　

    ISSN： 0287-4547

  84. Microbiologically Induced Corrosive Properties of the Titanium Surface

    A. Fukushima, G. Mayanagi, K. Nakajo, K. Sasaki, N. Takahashi

    JOURNAL OF DENTAL RESEARCH　93　(5)　525-529　2014/05

    DOI： 10.1177/0022034514524782 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  85. Rapid and Sensitive PCR-Dipstick DNA Chromatography for Multiplex Analysis of the Oral Microbiota

    Lingyang Tian, Takuichi Sato, Kousuke Niwa, Mitsuo Kawase, Anne C. R. Tanner, Nobuhiro Takahashi

    BIOMED RESEARCH INTERNATIONAL　2014　2014

    DOI： 10.1155/2014/180323 　

    ISSN： 2314-6133

    eISSN： 2314-6141

  86. Effect of fluoride-releasing restorative materials on bacteria-induced pH fall at the bacteria-material interface: An in vitro model study

    Gen Mayanagi, Koei Igarashi, Jumpei Washio, Hitomi Domon-Tawaraya, Nobuhiro Takahashi

    JOURNAL OF DENTISTRY　42　(1)　15-20　2014/01

    DOI： 10.1016/j.jdent.2013.11.006 　

    ISSN： 0300-5712

    eISSN： 1879-176X

  87. 東北大学における災害歯科学への取り組み

    冨士 岳志, 鈴木 敏彦, 坪井 明人, 小坂 健, 笹野 泰之, 高橋 信博, 佐々木 啓一

    みちのく歯学会雑誌　44　(1-2)　22-23　2013/12

    Publisher: 東北地区歯科医学会

    ISSN： 0385-0099

  88. Fluoride-sensitivity of growth and acid production of oral Actinomyces: comparison with oral Streptococcus

    Junko Kawashima, Kazuko Nakajo, Jumpei Washio, Gen Mayanagi, Hidetoshi Shimauchi, Nobuhiro Takahashi

    MICROBIOLOGY AND IMMUNOLOGY　57　(12)　797-804　2013/12

    DOI： 10.1111/1348-0421.12098 　

    ISSN： 0385-5600

    eISSN： 1348-0421

  89. A high-sensitive and non-radioisotopic fluorescence dye method for evaluating bacterial adhesion to denture materials

    Yoko Sakuma, Jumpei Washio, Keiichi Sasaki, Nobuhiro Takahashi

    DENTAL MATERIALS JOURNAL　32　(4)　585-591　2013/08

    DOI： 10.4012/dmj.2013-060 　

    ISSN： 0287-4547

  90. 東北大学歯学部・歯学研究科における新機軸の教育研究の展開(解説),

    佐々木 啓一, 竹内 裕尚, 天雲 太一, 洪 光, 真柳 弦, 西岡 貴志, 鈴木 治, 高橋 信博, 小坂 健

    みちのく歯学会雑誌　44　(1-2)　17-21　2013

    ISSN： 0385-0099

  91. Divalent Cations Enhance Fluoride Binding to Streptococcus mutans and Streptococcus sanguinis Cells and Subsequently Inhibit Bacterial Acid Production

    H. Domon-Tawaraya, K. Nakajo, J. Washio, T. Ashizawa, T. Ichino, H. Sugawara, S. Fukumoto, N. Takahashi

    CARIES RESEARCH　47　(2)　141-149　2013

    DOI： 10.1159/000344014 　

    ISSN： 0008-6568

  92. Dental caries from a molecular microbiological perspective

    B. Nyvad, W. Crielaard, A. Mira, N. Takahashi, D. Beighton

    Caries Research　47　(2)　89-102　2013

    DOI： 10.1159/000345367 　

    ISSN： 0008-6568

  93. Effects of a coating resin containing S-PRG filler to prevent demineralization of root surfaces

    Sai Ma, Satoshi Imazato, Ji-hua Chen, Gen Mayanagi, Nobuhiro Takahashi, Takuya Ishimoto, Takayoshi Nakano

    DENTAL MATERIALS JOURNAL　31　(6)　909-915　2012/11

    DOI： 10.4012/dmj.2012-061 　

    ISSN： 0287-4547

  94. Quantification and identification of bacteria in acrylic resin dentures and dento-maxillary obturator-prostheses

    Yasuhisa Takeuchi, Kazuko Nakajo, Takuichi Sato, Shigeto Koyama, Keiichi Sasaki, Nobuhiro Takahashi

    AMERICAN JOURNAL OF DENTISTRY　25　(3)　171-175　2012/06

    ISSN： 0894-8275

  95. Microflora Profiling of Infected Root Canal before and after Treatment Using Culture-Independent Methods

    Yasuhiro Ito, Takuichi Sato, Keiko Yamaki, Gen Mayanagi, Kazuhiro Hashimoto, Hidetoshi Shimauchi, Nobuhiro Takahashi

    JOURNAL OF MICROBIOLOGY　50　(1)　58-62　2012/02

    DOI： 10.1007/s12275-012-0459-4 　

    ISSN： 1225-8873

  96. Metabolomic approach to oral biofilm characterization-A future direction of biofilm research

    Nobuhiro Takahashi, Jumpei Washio, Gen Mayanagi

    Journal of Oral Biosciences　54　(3)　138-143　2012

    Publisher: Japanese Association for Oral Biology

    DOI： 10.1016/j.job.2012.02.005 　

    ISSN： 1349-0079

  97. Microbial composition of dental plaque microflora on first molars with orthodontic bands and brackets, and the acidogenic potential of these bacteria

    Ryo Komori, Takuichi Sato, Teruko Takano-Yamamoto, Nobuhiro Takahashi

    Journal of Oral Biosciences　54　(2)　107-112　2012

    Publisher: Japanese Association for Oral Biology

    DOI： 10.1016/j.job.2012.01.009 　

    ISSN： 1349-0079

  98. Cultivable anaerobic microbiota of infected root canals

    Takuichi Sato, Keiko Yamaki, Naoko Ishida, Kazuhiro Hashimoto, Yasuhisa Takeuchi, Megumi Shoji, Emika Sato, Junko Matsuyama, Hidetoshi Shimauchi, Nobuhiro Takahashi

    International Journal of Dentistry　2012

    DOI： 10.1155/2012/609689 　

    ISSN： 1687-8728 1687-8736

  99. Rapid quantification of bacteria in infected root canals using fluorescence reagents and a membrane filter: A pilot study on its clinical application to the evaluation of the outcomes of endodontic treatment

    Takuichi Sato, Keiko Yamaki, Naoko Ishida, Megumi Shoji, Emika Sato, Yuki Abiko, Kazuhiro Hashimoto, Yasuhisa Takeuchi, Junko Matsuyama, Hidetoshi Shimauchi, Nobuhiro Takahashi

    International Journal of Dentistry　2012

    DOI： 10.1155/2012/172935 　

    ISSN： 1687-8728 1687-8736

    eISSN： 1687-8736

  100. Evaluation of pH at the Bacteria-Dental Cement Interface

    G. Mayanagi, K. Igarashi, J. Washio, K. Nakajo, H. Domon-Tawaraya, N. Takahashi

    JOURNAL OF DENTAL RESEARCH　90　(12)　1446-1450　2011/12

    DOI： 10.1177/0022034511423392 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  101. Metabolomic Effects of Xylitol and Fluoride on Plaque Biofilm in Vivo

    N. Takahashi, J. Washio

    JOURNAL OF DENTAL RESEARCH　90　(12)　1463-1468　2011/12

    DOI： 10.1177/0022034511423395 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  102. Profiling of dental plaque microflora on root caries lesions and the protein-denaturing activity of these bacteria Peer-reviewed

    Kazuhiro Hashimoto, Takuichi Sato, Hidetoshi Shimauchi, Nobuhiro Takahashi

    AMERICAN JOURNAL OF DENTISTRY　24　(5)　295-299　2011/10

    ISSN： 0894-8275

  103. Effects of the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on bacterial viability and metabolism

    Naomi Izutani, Satoshi Imazato, Kazuko Nakajo, Nobuhiro Takahashi, Yusuke Takahashi, Shigeyuki Ebisu, Roy R. B. Russell

    EUROPEAN JOURNAL OF ORAL SCIENCES　119　(2)　175-181　2011/04

    DOI： 10.1111/j.1600-0722.2011.00817.x 　

    ISSN： 0909-8836

  104. The Role of Bacteria in the Caries Process: Ecological Perspectives

    N. Takahashi, B. Nyvad

    JOURNAL OF DENTAL RESEARCH　90　(3)　294-303　2011/03

    DOI： 10.1177/0022034510379602 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  105. Responses of infected dental pulp to αTCP-containing antimicrobials in rat molars

    Takuichi Sato, Shin-ichi Kenmotsu, Kuniko Nakakura-Ohshima, Nobuhiro Takahashi, Hayato Ohshima

    Archives of Histology and Cytology　73　(4+5)　165-175　2011

    Publisher: International Society of Histology & Cytology

    DOI： 10.1679/aohc.73.165 　

    ISSN： 0914-9465

    eISSN： 1349-1717

    More details Close

    α-tricalcium phosphate (a TCP) with the addition of antimicrobials such as ciprofloxacin, metronidazole, and cefaclor (3Mix) has been applied to sterilize the infected dentin and pulp in vivo. Both clinical and animal experiments have shown that 3Mix is effective for sterilizing infected tissues. However, the responses of the infected dental pulp to 3Mix remain to be fully determined at the cellular level. This study aims to clarify the responses of neural elements and immune cells to antimicrobials during the healing process of infected pulp using immunohistochemistry for protein gene product (PGP) 9.5 and class II major histocompatibility complex molecules using both light and electron microscopy. An artificial pulp exposure was prepared on the maxillary molar of 14-week-old rats and maintained without any treatment for 12 - 24 h. Subsequently, the exposed pulp was covered with αTCP or α TCP containing 3Mix, followed with glass ionomer cement. A pulp abscess lacking both dendritic cells and PGP 9.5-reactive nerve fibers was induced after pulp capping with α TCP; in contrast, numerous dendritic cells accumulated along the pulp-dentin border followed by the differentiation of odontoblast - like cells and matrix deposition after the application of α TCP containing 3Mix. PGP 9.5-reactive nerve fibers were also densely distributed and surrounded the accumulated dendritic cells in the medial dental pulp beneath α TCP containing 3Mix. The findings indicate that the application of α TCP containing 3Mix to the infected pulp induces an intense accumulation of dendritic cells, suggesting that these cells play crucial roles in the differentiation of odontoblast like cells under pathological conditions. ? 2011, International Society of Histology and Cytology. All rights reserved.

  106. Detection and identification of non-Candida albicans species in human oral lichen planus

    Mika Masaki, Takuichi Sato, Yumiko Sugawara, Takashi Sasano, Nobuhiro Takahashi

    MICROBIOLOGY AND IMMUNOLOGY　55　(1)　66-70　2011/01

    DOI： 10.1111/j.1348-0421.2010.00285.x 　

    ISSN： 0385-5600

  107. Metabolomics of Supragingival Plaque and Oral Bacteria

    N. Takahashi, J. Washio, G. Mayanagi

    JOURNAL OF DENTAL RESEARCH　89　(12)　1383-1388　2010/12

    DOI： 10.1177/0022034510377792 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  108. Ammonia Concentration and pH-lowering Activity of Marginal Dental Plaque from Teeth with and without Periodontitis

    KUMAGAMI Takahiro, SHIMIZU Koichi, IGARASHI Koei, TAKAHASHI Nobuhiro

    Journal of dental health　60　(5)　563-568　2010/10/30

    Publisher: The Japanese Society for Dental Health

    ISSN： 0023-2831

    More details Close

    While the pH-lowering activity and ammonia productivity of dental plaque are known to be associated with caries, the association with periodontal diseases is not clear. Thus, the pH-lowering activity and ammonia concentration of dental plaque were compared between teeth with and without periodontitis. Plaque samples (approximately 2.4μL) were obtained from marginal areas of sound buccal surfaces of upper first or second permanent molars of 71 subjects, and the probing depth (PD) and bleeding on probing (BOP) of the same tooth were recorded. Samples were measured for minimum pH after glucose addition and ammonia concentration. The ammonia concentration in subjects with PD&ge;3mm and BOP(+)(6.81±3.22mM) was significantly higher than in subjects with PD&ge;3mm and BOP(-)(3.50±2.38mM) and subjects with PD<3mm and BOP(-)(2.92±2.23mM). The minimum pH was in the order of subjects with PD&ge;3mm and BOP(+)(5.28±0.57), PD&ge;3mm and BOP(-)(5.54±0.74), and PD<3mm and BOP(-)(5.75±0.76), although there was no significant difference between subject groups. In addition, the ammonia concentration was weakly correlated with the minimum pH in each subject group. Marginal plaque from teeth with PD&ge;3mm and/or BOP(+) contained a significantly higher concentration of ammonia, indicating that periodontitis increases the ammonia level in marginal plaque. The inverse association between the ammonia concentration and minimum pH may be due to the metabolic characteristics of microbial flora in marginal dental plaque.

  109. Profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time PCR

    Y. Abiko, T. Sato, G. Mayanagi, N. Takahashi

    JOURNAL OF PERIODONTAL RESEARCH　45　(3)　389-395　2010/06

    DOI： 10.1111/j.1600-0765.2009.01250.x 　

    ISSN： 0022-3484

  110. Short-term effect of single NaF-mouthrinse on glucose-induced pH fall in dental plaque Peer-reviewed

    Kazuko Nakajo, Tomofumi Asanoumi, Akinobu Shibata, Yoko Yagishita, Kazuo Kato, Nobuhiro Takahashi

    INTERFACE ORAL HEALTH SCIENCE 2009　267-+　2010

    DOI： 10.1007/978-4-431-99644-6_73 　

  111. Short-time effect of fluoride on acid production by Streptococcus mutans Peer-reviewed

    Hitomi Domon, Kazuko Nakajo, Jumpei Washio, Harumi Miyasawa-Hori, Satoshi Fukumoto, Nobuhiro Takahashi

    INTERFACE ORAL HEALTH SCIENCE 2009　269-+　2010

    DOI： 10.1007/978-4-431-99644-6_74 　

  112. Experimental Ti-Ag alloys inhibit biofilm formation Peer-reviewed

    Masatoshi Takahashi, Kazuko Nakajo, Nobuhiro Takahashi, Keiichi Sasaki, Osamu Okuno

    INTERFACE ORAL HEALTH SCIENCE 2009　283-+　2010

    DOI： 10.1007/978-4-431-99644-6_80 　

  113. Hydrogen-sulfide production from various substrates by oral Veillonella and effects of lactate on the production Peer-reviewed

    Jumpei Washio, Yoko Sakuma, Yuko Shimada, Nobuhiro Takahashi

    INTERFACE ORAL HEALTH SCIENCE 2009　250-251　2010

    DOI： 10.1007/978-4-431-99644-6_66 　

  114. Isolation and identification of viable bacteria within acrylic resin denture bases Peer-reviewed

    Takeuchi Yasuhisa, Nakajo Kazuko, Sato Takuichi, Sakuma Yoko, Sasaki Keiichi, Takahashi Nobuhiro

    INTERFACE ORAL HEALTH SCIENCE 2009　230-+　2010

    DOI： 10.1007/978-4-431-99644-6_58 　

  115. New quantitative fluorometry for evaluating oral bacterial adhesion to biomaterials Peer-reviewed

    Sakuma Yoko, Washio Jumpei, Takeuchi Yasuhisa, Sasaki Keiichi, Takahashi Nobuhiro

    INTERFACE ORAL HEALTH SCIENCE 2009　215-+　2010

    DOI： 10.1007/978-4-431-99644-6_52 　

  116. Challenge to metabolomics of oral biofilm -From "What are they?" to "What are they doing?"-

    Jumpei Washio, Gen Mayanagi, Nobuhiro Takahashi

    Journal of Oral Biosciences　52　(3)　225-232　2010

    Publisher: Japanese Association for Oral Biology

    DOI： 10.2330/joralbiosci.52.225 　

    ISSN： 1349-0079

  117. Autoactivation of proteolytic activity in human whole saliva

    Yoshitada Miyoshi, Makoto Watanabe, Nobuhiro Takahashi

    Journal of Oral Biosciences　52　(4)　402-408　2010

    Publisher: Japanese Association for Oral Biology

    DOI： 10.2330/joralbiosci.52.402 　

    ISSN： 1349-0079

  118. Resistance to Acidic Environments of Caries-Associated Bacteria: Bifidobacterium dentium and Bifidobacterium longum

    K. Nakajo, N. Takahashi, D. Beighton

    CARIES RESEARCH　44　(5)　431-437　2010

    DOI： 10.1159/000318582 　

    ISSN： 0008-6568

  119. Transient acid-impairment of growth ability of oral Streptococcus, Actinomyces, and Lactobacillus: a possible ecological determinant in dental plaque

    M. Horiuchi, J. Washio, H. Mayanagi, N. Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　24　(4)　319-324　2009/08

    DOI： 10.1111/j.1399-302X.2009.00517.x 　

    ISSN： 0902-0055

  120. Fluoride released from glass-ionomer cement is responsible to inhibit the acid production of caries-related oral streptococci

    Kazuko Nakajo, Satoshi Imazato, Yusuke Takahashi, Wakako Kiba, Shigeyuki Ebisu, Nobuhiro Takahashi

    DENTAL MATERIALS　25　(6)　703-708　2009/06

    DOI： 10.1016/j.denta1.2008.10.014 　

    ISSN： 0109-5641

    eISSN： 1879-0097

  121. Effects of alpha-Amylase and Its Inhibitors on Acid Production from Cooked Starch by Oral Streptococci

    S. Aizawa, H. Miyasawa-Hori, K. Nakajo, J. Washio, H. Mayanagi, S. Fukumoto, N. Takahashi

    CARIES RESEARCH　43　(1)　17-24　2009

    DOI： 10.1159/000189703 　

    ISSN： 0008-6568

    eISSN： 1421-976X

  122. Mutual induction of noncollagenous bone proteins at the interface between epithelial cells and fibroblasts from human periodontal ligament Peer-reviewed

    M. Shimonishi, J. Hatakeyama, Y. Sasano, N. Takahashi, M. Komatsu, M. Kikuchi

    JOURNAL OF PERIODONTAL RESEARCH　43　(1)　64-75　2008/02

    DOI： 10.1111/j.1600-0765.2007.00995.x 　

    ISSN： 0022-3484

  123. Chairside evaluation of pH-lowering activity and lactic acid production of dental plaque: Correlation with caries experience and incidence in preschool children

    Koichi Shimizu, Koei Igarashi, Nobuhiro Takahashi

    QUINTESSENCE INTERNATIONAL　39　(2)　151-158　2008/02

    ISSN： 0033-6572

  124. Caries Ecology Revisited: Microbial Dynamics and the Caries Process

    N. Takahashi, B. Nyvad

    CARIES RESEARCH　42　(6)　409-418　2008

    DOI： 10.1159/000159604 　

    ISSN： 0008-6568

  125. Profiling of subgingival plaque biofilm microflora of healthy and periodontitis subjects by real-time PCR. Invited Peer-reviewed

    Yuki Abiko, Takuichi Sato Gen, Mayanagi Nobuhiro Takahashi

    Interface Oral Health Science 2007　213-218　2007/12

  126. Hydrogen sulfide production by oral Veillonella: effects of substrate and environmental pH. Invited Peer-reviewed

    Jumpei Washio, Shohei Matoba, Tomoyuki Seki Naohide Yamamoto, Miou Yamamoto, Nobuhiro Takahashi

    Interface Oral Health Science 2007　219-224　2007/12

  127. Real-time PCR analyses of genera Veillonella and Streptococcus in healthy supragingival plaque biofilm microflora of children. Peer-reviewed

    Junko Matsuyama, Takuichi Sato, Nobuhiro, Takahashi, Michiko Sato, Etsuro Hoshino

    Interface Oral Health Science 2007　255-256　2007/12

  128. Inhibitory effects of maltotriitol on the growth and adhesion of mutans streptococci. Peer-reviewed

    Harumi Miyasawa-Hori, Shizuko Aizawa Jumpei Washio Nobuhiro Takahashi

    Interface Oral Health Science 2007　257-258　2007/12

  129. The effect of amylase and its inhibitors on acid production from starch by Streptococcus mutans and Streptococcus sanguinis Peer-reviewed

    Shizuko Aizawa, Harumi Miyasawa-Hori, Haruhide Mayanagi, Nobuhiro Takahashi

    Interface Oral Health Science 2007　261-262　2007/12

  130. Fluoride ion released from glass-ionomer cement is responsible to inhibit the acid production of caries-related oral streptococci. Peer-reviewed

    Kazuko Nakajo, Yusuke, Takahashi Wakako Kiba, Satoshi Imazato, Nobuhiro Takahashi

    Interface Oral Health Science 2007　263-264　2007/12

  131. Microflora profiling of root canal utilizing real-time PCR and cloning-sequence analyses based on 16S rRNA genes -differences between before and after root canal treatments. Peer-reviewed

    Yasuhiro Ito, Takuichi Sato Gen, Mayanagi Keiko, Yamaki Hidetoshi, Shimauchi Nobuhiro Takahashi

    Interface Oral Health Science 2007　265-266　2007/12

  132. Detection of periodontopathic bacteria in periodontal pockets by nested polymerase chain reaction. Peer-reviewed

    Takuichi Sato, Yuki Abiko Gen, Mayanagi Junko, Matsuyama Nobuhiro Takahashi

    Interface Oral Health Science 2007　267-268　2007/12

  133. Candida species as members of oral microflora in oral lichen planus. Peer-reviewed

    Mika Masaki, Takuichi Sato, Yumiko Sugawara, Takashi Sasano, Nobuhiro Takahashi

    Interface Oral Health Science 2007　273-274　2007/12

  134. Gelatinase activity in human saliva and its fluctuation in the oral cavity. Peer-reviewed

    Yoshitada Miyoshi Makoto Watanabe, Nobuhiro Takahashi

    Interface Oral Health Science 2007　291-292　2007/12

  135. In vitro differentiation of epithelial cells cultured from human periodontal ligament Peer-reviewed

    M. Shimonishi, J. Hatakeyama, Y. Sasano, N. Takahashi, T. Uchida, M. Kikuchi, M. Komatsu

    JOURNAL OF PERIODONTAL RESEARCH　42　(5)　456-465　2007/10

    DOI： 10.1111/j.1600-0765.2007.00969.x 　

    ISSN： 0022-3484

  136. Nested PCR for the sensitive detection of cariogenic bacteria Invited

    Sato T, Matsuyama J, Mayanagi G, Abiko Y, Kato K, Takahashi N

    Cariology Today　3-4　(1)　17-20　2007/03

  137. Profiling of bacterial flora in crevices around titanium orthodontic anchor plates Peer-reviewed

    Renya Sato, Takuichi Sato, Ichiro Takahashi, Junji Sugawara, Nobuhiro Takahashi

    CLINICAL ORAL IMPLANTS RESEARCH　18　(1)　21-26　2007/02

    DOI： 10.1111/j.1600-0501.2006.01294.x 　

    ISSN： 0905-7161

  138. Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis Peer-reviewed

    K. Nakajo, R. Komori, S. Ishikawa, T. Ueno, Y. Suzuki, Y. Iwami, N. Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　21　(5)　283-288　2006/10

    DOI： 10.1111/j.1399-302X.2006.00289.x 　

    ISSN： 0902-0055

  139. Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and articular cartilage in rats Peer-reviewed

    Hidetoshi Mitani, Ichiro Takahashi, Kazuyuki Onodera, Jin-Wan Bae, Takuichi Sato, Nobuhiro Takahashi, Yasuyuki Sasano, Kaoru Igarashi, Hideo Mitani

    HISTOCHEMISTRY AND CELL BIOLOGY　126　(3)　371-380　2006/09

    DOI： 10.1007/s00418-006-0171-8 　

    ISSN： 0948-6143

  140. Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains and the biochemical mechanism Peer-reviewed

    H. Miyasawa-Hori, S. Aizawa, N. Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　21　(4)　201-205　2006/08

    DOI： 10.1111/j.1399-302X.2006.00273.x 　

    ISSN： 0902-0055

  141. Quantication and detection of bacteria from postoperative maxillary cyst by polymerase chain reaction. (vol 20, pg 333, 2005)

    M Yamaura, T Sato, S Echigo, N Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　21　(2)　136-136　2006/04

    ISSN： 0902-0055

  142. 培養ヒト歯根膜由来上皮細胞と線維芽細胞の境界面におけるオステオポンチンおよびオステオカルシンの発現 Peer-reviewed

    佐藤充太, 下西充, 高橋信博, 小松正志

    日本歯科保存学雑誌　49　(1)　92-98　2006/02

    Publisher:

    ISSN： 0387-2343

    eISSN： 2188-0808

  143. Quantification and detection of bacteria from postoperative maxillary cyst by polymerase chain reaction Peer-reviewed

    M Yamaura, T Sato, S Echigo, N Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　20　(6)　333-338　2005/12

    DOI： 10.1111/j.1399-302X.2005.00229.x 　

    ISSN： 0902-0055

  144. Microbial ecosystem in the oral cavity: Metabolic diversity in an ecological niche and its relationship with oral diseases Invited Peer-reviewed

    Nobuhiro Takahashi

    International Congress Series　1284　103-112　2005/09

    DOI： 10.1016/j.ics.2005.06.071 　

    ISSN： 0531-5131

  145. Microflora profiling of subgingival and supragingival plaque of healthy and periodontitis subjects by nested PCR Peer-reviewed

    G. Mayanagi, T. Sato, H. Shimauchi, N. Takahashi

    International Congress Series　1284　195-196　2005/09

    DOI： 10.1016/j.ics.2005.06.069 　

    ISSN： 0531-5131

  146. Microflora profiling of crevices around titanium orthodontic anchor plates Peer-reviewed

    R. Sato, T. Sato, I. Takahashi, J. Sugawara, N. Takahashi

    International Congress Series　1284　197-198　2005/09

    DOI： 10.1016/j.ics.2005.06.061 　

    ISSN： 0531-5131

  147. Relationship between hydrogen sulfide-producing bacteria of the tongue coating and oral malodor Peer-reviewed

    J. Washio, T. Sato, K. Ikawa, N. Tanda, M. Iwakura, T. Koseki, N. Takahashi

    International Congress Series　1284　199-200　2005/09

    DOI： 10.1016/j.ics.2005.06.068 　

    ISSN： 0531-5131

  148. The resistance to acidic and alkaline environments of endodontic pathogen Enterococcus faecalis Peer-reviewed

    K. Nakajo, Y. Iwami, R. Komori, S. Ishikawa, T. Ueno, Y. Suzuki, N. Takahashi

    International Congress Series　1284　191-192　2005/09

    DOI： 10.1016/j.ics.2005.06.060 　

    ISSN： 0531-5131

  149. Density profiles of total bacteria and S. mutans within dental plaque treated with stannous fluoride gel Peer-reviewed

    K. Kato, K. Fukui, H. Nakagaki, T. Sato, N. Takahashi

    International Congress Series　1284　185-186　2005/09

    DOI： 10.1016/j.ics.2005.07.010 　

    ISSN： 0531-5131

  150. PCR for detection of mutans streptococci in human dental plaque Peer-reviewed

    J. Matsuyama, T. Sato, J. Washio, G. Mayanagi, Y. Ito, Y. Abiko, K. Hashimoto, H. Miyasawa-Hori, K. Nakajo, K. Kato, N. Takahashi

    International Congress Series　1284　158-162　2005/09

    DOI： 10.1016/j.ics.2005.07.030 　

    ISSN： 0531-5131

  151. Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains, and its biochemical mechanism Peer-reviewed

    H. Miyasawa-Hori, S. Aizawa, N. Takahashi

    International Congress Series　1284　187-188　2005/09

    DOI： 10.1016/j.ics.2005.06.049 　

    ISSN： 0531-5131

  152. Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodour Peer-reviewed

    J Washio, T Sato, T Koseki, N Takahashi

    JOURNAL OF MEDICAL MICROBIOLOGY　54　(9)　889-895　2005/09

    DOI： 10.1099/jmm.0.46118-0 　

    ISSN： 0022-2615

  153. Quantification of total bacterial DNA by real-time PCR and detection of bacteria by nested PCR from postoperative maxillary cyst Peer-reviewed

    M. Yamaura, T. Sato, S. Echigo, N. Takahashi

    International Congress Series　1284　193-194　2005/09

    DOI： 10.1016/j.ics.2005.06.062 　

    ISSN： 0531-5131

  154. Expression of non-collagenous bone proteins in cultured epithelial rests of Malassez

    M. Shimonishi, N. Takahashi, M. Komatsu

    International Congress Series　1284　81-82　2005/09

    DOI： 10.1016/j.ics.2005.06.046 　

    ISSN： 0531-5131

  155. インターフェイス口腔健康科学 -東北大学大学院歯学研究科が掲げる次世代の歯学- Peer-reviewed

    高橋信博

    東北大学歯学雑誌　24　(1)　33-34　2005/04

  156. Expression of type IV collagen and laminin at the interface between epithelial cells and fibroblasts from human periodontal ligament Peer-reviewed

    M Shimonishi, J Sato, N Takahashi, M Komatsu

    EUROPEAN JOURNAL OF ORAL SCIENCES　113　(1)　34-40　2005/02

    DOI： 10.1111/j.1600-0722.2004.00178.x 　

    ISSN： 0909-8836

  157. Synergistic inhibition by combination of fluoride and xylitol on glycolysis by mutans streptococci and its biochemical mechanism Peer-reviewed

    H Maehara, Y Iwami, H Mayanagi, N Takahashi

    CARIES RESEARCH　39　(6)　521-528　2005

    DOI： 10.1159/000088190 　

    ISSN： 0008-6568

  158. Detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects Peer-reviewed

    G Mayanagi, T Sato, H Shimauchi, N Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　19　(6)　379-385　2004/12

    DOI： 10.1111/j.1399-302x.2004.00172.x 　

    ISSN： 0902-0055

  159. PTC gene mutations and expression of SHH, PTC, SMO and GLI-1 in odontogenic keratocysts Peer-reviewed

    K Ohki, H Kumamoto, R Ichinohasama, T Sato, N Takahashi, K Ooya

    INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY　33　(6)　584-592　2004/09

    DOI： 10.1016/j.ijom.2004.01.013 　

    ISSN： 0901-5027

    eISSN： 1399-0020

  160. K-Ras gene status and expression of Ras/mitogen-activated protein kinase (MAPK) signaling molecules in ameloblastomas Peer-reviewed

    H Kumamoto, N Takahashi, K Ooya

    JOURNAL OF ORAL PATHOLOGY & MEDICINE　33　(6)　360-367　2004/07

    DOI： 10.1111/j.1600-0714.2004.00141.x 　

    ISSN： 0904-2512

  161. p53 gene status and expression of p53, MDM2, and p14(ARF) proteins in ameloblastomas Peer-reviewed

    H Kumamoto, T Izutsu, K Ohki, N Takahashi, K Ooya

    JOURNAL OF ORAL PATHOLOGY & MEDICINE　33　(5)　292-299　2004/05

    DOI： 10.1111/j.0904-2512.2004.00044.x 　

    ISSN： 0904-2512

  162. A method for mapping the distribution pattern of cariogenic streptococci within dental plaque in vivo Peer-reviewed

    K Kato, T Sato, N Takahashi, K Fukui, K Yamamoto, H Nakagaki

    CARIES RESEARCH　38　(5)　448-453　2004

    DOI： 10.1159/000079626 　

    ISSN： 0008-6568

  163. K-Ras gene status and expression of Ras/mitogen-activated protein kinase (MAPK) signaling molecules in ameloblastomas

    Hiroyuki Kumamoto, Nobuhiro Takahashi, Kiyoshi Ooya

    Journal of Oral Pathology and Medicine　33　(6)　360-367　2004

    Publisher: Blackwell Publishing Ltd

    DOI： 10.1111/j.1600-0714.2004.00141.x 　

    ISSN： 0904-2512

  164. Xylitol inhibition of acid production and growth of mutans streptococci in the presence of various dietary sugars under strictly anaerobic conditions Peer-reviewed

    H Kakuta, Y Iwami, H Mayanagi, N Takahashi

    CARIES RESEARCH　37　(6)　404-409　2003/11

    DOI： 10.1159/000073391 　

    ISSN： 0008-6568

  165. Fermentation of five sucrose isomers by human dental plaque bacteria Peer-reviewed

    J Matsuyama, T Sato, E Hoshino, T Noda, N Takahashi

    CARIES RESEARCH　37　(6)　410-415　2003/11

    DOI： 10.1159/000073392 　

    ISSN： 0008-6568

  166. Identification of mutans streptococci by restriction fragment length polymorphism analysis of polymerase chain reaction-amplified 16S ribosomal RNA genes Peer-reviewed

    T Sato, JP Hu, K Ohki, M Yamaura, J Washio, J Matsuyama, N Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　18　(5)　323-326　2003/10

    DOI： 10.1034/j.1399-302X.2003.00095.x 　

    ISSN： 0902-0055

  167. Biochemical and functional properties of a pyruvate formate-lyase (PFL)-activating system in Streptococcus mutans Peer-reviewed

    S Takahashi-Abbe, K Abe, N Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　18　(5)　293-297　2003/10

    DOI： 10.1034/j.1399-302X.2003.00081.x 　

    ISSN： 0902-0055

  168. Xylitol inhibition of anaerobic acid production by Streptococcus mutans at various pH levels Peer-reviewed

    H Miyasawa, Y Iwami, H Mayanagi, N Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　18　(4)　215-219　2003/08

    DOI： 10.1034/j.1399-302X.2003.00068.x 　

    ISSN： 0902-0055

  169. Proteolysis of ICAM-1 on human oral epithelial cells by cysteine proteinase, (Gingipains) from Porphyromonas gingivalis leading to down-regulation of neutrophil adhesion. International-journal International-coauthorship Peer-reviewed

    Tada H, Sugawara S, Nemoto E, Takahashi N, Imamura T, Potempa J, Travis J, Shimauchi H, Takada H

    Journal of Dental Research　82　(10)　B41-B41　2003/06

    DOI： 10.1177/154405910308201007 　

    ISSN： 0022-0345

  170. Acid-neutralizing activity during amino acid fermentation by Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum Peer-reviewed

    N Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　18　(2)　109-113　2003/04

    DOI： 10.1034/j.1399-302X.2003.00054.x 　

    ISSN： 0902-0055

  171. Nested PCR for detection of mutans streptococci in dental plaque Peer-reviewed

    T Sato, J Matsuyama, T Kumagai, G Mayanagi, M Yamaura, J Washio, N Takahashi

    LETTERS IN APPLIED MICROBIOLOGY　37　(1)　66-69　2003

    DOI： 10.1046/j.1472-765X.2003.01359.x 　

    ISSN： 0266-8254

  172. Rapid identification of cariogenic bacteria by 16S rRNA genes PCR-RFLP analysis Invited

    Sato T, Hu JP, Matsuyama J, Takahashi N

    Cariology Today　2　(1)　8-13　2002/09

  173. nested PCR を利用した歯垢内の齲蝕関連菌の層別分布の分析

    加藤 一夫, 佐藤 拓一, 高橋 信博, 山本 恭子, 福井 敬子, 中垣 晴男

    口腔衛生学会雑誌　52　(4)　568-569　2002/08/15

    ISSN： 0023-2831

  174. Intracellular and extracellular pHs of Streptococcus mutans after addition of acids: loading and efflux of a fluorescent pH indicator in streptococcal cells Peer-reviewed

    Y Iwami, K Kawarada, Kojima, I, H Miyasawa, H Kakuta, H Mayanagi, N Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　17　(4)　239-244　2002/08

    DOI： 10.1034/j.1399-302X.2002.170406.x 　

    ISSN： 0902-0055

  175. Biochemical Approach to Dental Plaque Ecosystem

    Nobuhiro Takahashi, Division of Oral Biochemistry Department of Oral Biology Tohoku University Graduate School of Dentistry

    21　(1)　18-32　2002/06/01

    ISSN： 0287-3915

  176. Proteolysis of CD14 on human gingival fibroblasts by arginine-specific cysteine proteinases from Porphyromonas gingivalis leading to down-regulation of lipopolysaccharide-induced interleukin-8 production Peer-reviewed

    H Tada, S Sugawara, E Nemoto, N Takahashi, T Imamura, J Potempa, J Travis, H Shimauchi, H Takada

    INFECTION AND IMMUNITY　70　(6)　3304-3307　2002/06

    DOI： 10.1128/IAI.70.6.3304-3307.2002 　

    ISSN： 0019-9567

  177. Dipeptide utilization by the periodontal pathogens Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens and Fusobacterium nucleatum Peer-reviewed

    N Takahashi, T Sato

    ORAL MICROBIOLOGY AND IMMUNOLOGY　17　(1)　50-54　2002/02

    DOI： 10.1046/j.0902-0055.2001.00089.x 　

    ISSN： 0902-0055

  178. Effects of glucose on formation of cytotoxic end-products and proteolytic activity of Prevotella intermedia, Prevotella nigrescens and Porphyromonas gingivalis Peer-reviewed

    K Saito, N Takahashi, H Horiuchi, T Yamada

    JOURNAL OF PERIODONTAL RESEARCH　36　(6)　355-360　2001/12

    ISSN： 0022-3484

  179. Preferential utilization of dipeptides by Porphyromonas gingivalis Peer-reviewed

    N Takahashi, T Sato

    JOURNAL OF DENTAL RESEARCH　80　(5)　1425-1429　2001/05

    ISSN： 0022-0345

  180. Inhibitory effect of sorbitol on sugar metabolism of Streptococcus mutans in vitro and on acid production in dental plaque in vivo Peer-reviewed

    S Takahashi-Abbe, K Abbe, N Takahashi, Y Tamazawa, T Yamada

    ORAL MICROBIOLOGY AND IMMUNOLOGY　16　(2)　94-99　2001/04

    DOI： 10.1034/j.1399-302X.2001.016002094.x 　

    ISSN： 0902-0055

  181. 口腔内pH電極内臓法によるチューインガムの酸蝕性およびヒト歯垢における酸産生性の検討. Peer-reviewed

    阿部昌子, 玉澤佳純, 阿部一彦, 高橋信博

    日本食品新素材研究会誌　4　(2)　13-19　2001/04

  182. The time-course of acid excretion, levels of fluorescence dependent on cellular nicotinamide adenine nucleotide and glycolytic intermediates of Streptococcus mutans cells exposed and not exposed to air in the presence of glucose and sorbitol Peer-reviewed

    Y Iwami, S Takahashi-Abbe, N Takahashi, T Yamada, N Kano, H Mayanagi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　16　(1)　34-39　2001/02

    DOI： 10.1034/j.1399-302X.2001.160106.x 　

    ISSN： 0902-0055

  183. Effects of glucose on formation of cytotoxic end-products and proteolytic activity of Prevotella intermedia, Prevotella nigrescens and Porphyromonas gingivalis

    Kaoru Saito, Nobuhiro Takahashi, Hiroshi Horiuchi, Tadashi Yamada

    Journal of Periodontal Research　36　(6)　355-360　2001

    Publisher: Blackwell Munksgaard

    DOI： 10.1034/j.1600-0765.2001.360602.x 　

    ISSN： 0022-3484

  184. Preferential utilization of dipeptides by Porphyromonas gingivalis

    N. Takahashi, T. Sato

    Journal of Dental Research　80　(5)　1425-1429　2001

    Publisher: Intern. and American Associations for Dental Research

    DOI： 10.1177/00220345010800050801 　

    ISSN： 0022-0345

  185. 16S rRNA genes PCR-RFLP analysis for rapid identification of oral anaerobic gram-positive bacilli Peer-reviewed

    Sato T, Matsuyama J, Takahashi N

    International Journal of Oral Biology　25　(3)　87-91　2000/09

  186. Comparison between 16S rRNA genes PCR-RFLP analysis and biochemical tests for identification of Actinomyces naeslundii Peer-reviewed

    Matsuyama J, Sato T, Takahashi N

    International Journal of Oral Biology　25　(3)　83-86　2000/09

  187. Metabolic pathways for cytotoxic end product formation from glutamate- and aspartate-containing peptides by Porphyromonas gingivalis

    N Takahashi, T Sato, T Yamada

    JOURNAL OF BACTERIOLOGY　182　(17)　4704-4710　2000/09

    DOI： 10.1128/JB.182.17.4704-4710.2000 　

    ISSN： 0021-9193

  188. Characterization of the Streptococcus mutans pyruvate formate-lyase (PFL)-activating enzyme gene by complementary reconstitution of the in vitro PFL-reactivating system

    Y Yamamoto, Y Sato, S Takahashi-Abbe, N Takahashi, H Kizaki

    INFECTION AND IMMUNITY　68　(8)　4773-4777　2000/08

    DOI： 10.1128/IAI.68.8.4773-4777.2000 　

    ISSN： 0019-9567

  189. Glucose metabolism by Prevotella intermedia and Prevotella nigrescens

    N Takahashi, T Yamada

    ORAL MICROBIOLOGY AND IMMUNOLOGY　15　(3)　188-195　2000/06

    DOI： 10.1034/j.1399-302X.2000.150307.x 　

    ISSN： 0902-0055

  190. Pathways for amino acid metabolism by Prevotella intermedia and Prevotella nigrescens

    N Takahashi, T Yamada

    ORAL MICROBIOLOGY AND IMMUNOLOGY　15　(2)　96-102　2000/04

    DOI： 10.1034/j.1399-302X.2000.150205.x 　

    ISSN： 0902-0055

  191. Coptidis rhizoma inhibits growth and proteases of oral bacteria

    J. P. Hu, N. Takahashi, T. Yamada

    Oral Diseases　6　(5)　297-302　2000

    ISSN： 1354-523X

  192. Rate-limiting steps of glucose and sorbitol metabolism in streptococcus mutans cells exposed to air

    Y. Iwami, S. Takahashi-Abbe, N. Takahashi, K. Abbe, T. Yamada

    Oral Microbiology and Immunology　15　(5)　325-328　2000

    DOI： 10.1034/j.1399-302X.2000.150510.x 　

    ISSN： 0902-0055

  193. Functions of two types of NADH oxidases in energy metabolism and oxidative stress of Streptococcus mutans

    M Higuchi, Y Yamamoto, LB Poole, M Shimada, Y Sato, N Takahashi, Y Kamio

    JOURNAL OF BACTERIOLOGY　181　(19)　5940-5947　1999/10

    ISSN： 0021-9193

  194. Acid-induced acid tolerance and acidogenicity of non-mutans streptococci

    N Takahashi, T Yamada

    ORAL MICROBIOLOGY AND IMMUNOLOGY　14　(1)　43-48　1999/02

    DOI： 10.1034/j.1399-302X.1999.140105.x 　

    ISSN： 0902-0055

  195. Effects of pH on the glucose and lactate metabolisms by the washed cells of Actinomyces naeslundii under anaerobic and aerobic conditions

    N Takahashi, T Yamada

    ORAL MICROBIOLOGY AND IMMUNOLOGY　14　(1)　60-65　1999/02

    DOI： 10.1034/j.1399-302X.1999.140108.x 　

    ISSN： 0902-0055

  196. Glucose and lactate metabolism by actinomyces naeslundii

    N. Takahashi, T. Yamada

    Critical Reviews in Oral Biology and Medicine　10　(4)　487-503　1999

    Publisher: Intern. and American Associations for Dental Research

    DOI： 10.1177/10454411990100040501 　

    ISSN： 1045-4411

  197. Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length polymorphism

    T Sato, J Matsuyama, N Takahashi, M Sato, J Johnson, C Schachtele, E Hoshino

    ARCHIVES OF ORAL BIOLOGY　43　(3)　247-252　1998/03

    DOI： 10.1016/S0003-9969(98)00005-3 　

    ISSN： 0003-9969

  198. Acid tolerance and acid-neutralizing activity of Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum

    N Takahashi, K Saito, CF Schachtele, T Yamada

    ORAL MICROBIOLOGY AND IMMUNOLOGY　12　(6)　323-328　1997/12

    ISSN： 0902-0055

  199. Effects of acidification on growth and glycolysis of Streptococcus sanguis and Streptococcus mutans

    N. Takahashi, M. Horiuchi, T. Yamada

    Oral Microbiology and Immunology　12　(2)　72-76　1997

    Publisher: Blackwell Publishing Ltd

    DOI： 10.1111/j.1399-302X.1997.tb00620.x 　

    ISSN： 0902-0055

  200. Catabolic pathway for aerobic degradation of lactate by Actinomyces naeslundii

    N. Takahashi, T. Yamada

    Oral Microbiology and Immunology　11　(3)　193-198　1996

    Publisher: Blackwell Publishing Ltd

    DOI： 10.1111/j.1399-302X.1996.tb00357.x 　

    ISSN： 0902-0055

  201. Effect of acetate on sorbitol fermentation by oral lactobacilli

    N. Takahashi, S. Kalfas, T. Yamada

    Oral Microbiology and Immunology　10　(6)　349-354　1995

    DOI： 10.1111/j.1399-302X.1995.tb00165.x 　

    ISSN： 1399-302X 0902-0055

  202. Phosphorylating enzymes involved in glucose fermentation of Actinomyces naeslundii

    N. Takahashi, S. Kalfas, T. Yamada

    Journal of Bacteriology　177　(20)　5806-5811　1995

    Publisher: American Society for Microbiology

    DOI： 10.1128/jb.177.20.5806-5811.1995 　

    ISSN： 0021-9193

  203. Acid Profiles and pH of Carious Dentin in Active and Arrested Lesions

    S. Hojo, M. Komatsu, R. Okuda, N. Takahashi, T. Yamada

    Journal of Dental Research　73　(12)　1853-1857　1994

    DOI： 10.1177/00220345940730121001 　

    ISSN： 1544-0591 0022-0345

  204. Initial catabolism of sorbitol in Actinomyces naeslundii and Actinomyces viscosus

    S. Kalfas, N. Takahashi, T. Yamada

    Oral Microbiology and Immunology　9　(6)　372-375　1994

    DOI： 10.1111/j.1399-302X.1994.tb00288.x 　

    ISSN： 1399-302X 0902-0055

  205. The role of the succinate pathway in sorbitol fermentation by oral Actinomyces viscosus and Actinomyces naeslundii

    N. Takahashi, S. Kalfas, T. Yamada

    Oral Microbiology and Immunology　9　(4)　218-223　1994

    DOI： 10.1111/j.1399-302X.1994.tb00061.x 　

    ISSN： 1399-302X 0902-0055

  206. IMMUNOGLOBULINS IN MILK FROM COWS IMMUNIZED WITH ORAL STRAINS OF ACTINOMYCES, PREVOTELLA, PORPHYROMONAS, AND FUSOBACTERIUM

    N TAKAHASHI, G EISENHUTH, LEE, I, N LAIBLE, S BINION, C SCHACHTELE

    JOURNAL OF DENTAL RESEARCH　71　(8)　1509-1515　1992/08

    DOI： 10.1177/00220345920710080901 　

    ISSN： 0022-0345

  207. NONSPECIFIC ANTIBACTERIAL FACTORS IN MILK FROM COWS IMMUNIZED WITH HUMAN ORAL BACTERIAL PATHOGENS

    N TAKAHASHI, G EISENHUTH, LEE, I, C SCHACHTELE, N LAIBLE, S BINON

    JOURNAL OF DAIRY SCIENCE　75　(7)　1810-1820　1992/07

    DOI： 10.3168/jds.S0022-0302(92)77939-9 　

    ISSN： 0022-0302

  208. Stimulatory effect of bicarbonate on the glycolysis of Actinomyces viscosus and its biochemical mechanism

    N. Takahashi, T. Yamada

    Oral Microbiology and Immunology　7　(3)　165-170　1992

    DOI： 10.1111/j.1399-302X.1992.tb00530.x 　

    ISSN： 1399-302X 0902-0055

  209. Metabolism of intracellular polysaccharide in the cells of Streptococcus mutatis under strictly anaerobic conditions

    Nobuhiro Takahashi, Yoshimichi Iwami, Tadashi Yamada

    Oral Microbiology and Immunology　6　(5)　299-304　1991

    DOI： 10.1111/j.1399-302X.1991.tb00497.x 　

    ISSN： 1399-302X 0902-0055

  210. Acid Profile in Carious Dentin

    S. Hojo, N. Takahashi, T. Yamada

    Journal of Dental Research　70　(3)　182-186　1991

    DOI： 10.1177/00220345910700030501 　

    ISSN： 1544-0591 0022-0345

  211. Acid profiles and pH in carious dentin Peer-reviewed

    Sachiko Hojo, N.Takahashi, M.Komatsu, R.Okuda, T.Yamada

    Journal of Dental Research, Vol.69, Special lssue., pp.1045, 1990　Special lssue　1045-1045　1990

  212. Effect of pH on the Growth and Proteolytic Activity of Porphyromonas gingivalis and Bacteroides intermedius

    N. Takahashi, C. F. Schachtele

    Journal of Dental Research　69　(6)　1266-1269　1990

    DOI： 10.1177/00220345900690060801 　

    ISSN： 1544-0591 0022-0345

  213. DIFFERENCE IN AMOUNTS BETWEEN TITRATABLE ACID AND TOTAL CARBOXYLIC-ACIDS PRODUCED BY ORAL STREPTOCOCCI DURING SUGAR METABOLISM

    Y IWAMI, S HATA, N TAKAHASHI, T YAMADA

    JOURNAL OF DENTAL RESEARCH　68　(1)　16-19　1989/01

    DOI： 10.1177/00220345890680010101 　

    ISSN： 0022-0345

  214. Evalution of the acidogenic activity of dental plaque in vivo by an in-dwelling electrode method

    TAKAHASHI N., Nobuhiro Takahashi, Department of Oral Biochemistry Tohoku University School of Dentistry

    Tohoku Univ. Dent. J.　6　(2)　91-98　1987/12/01

    ISSN： 0287-3915

  215. Oxygen sensitivity of sugar metabolism and interconversion of pyruvate formate-lyase in intact cells of Streptococcus mutans and Streptococcus sanguis

    N. Takahashi, K. Abbe, S. Takahashi-Abbe, T. Yamada

    Infection and Immunity　55　(3)　652-656　1987

    ISSN： 0019-9567

Show all ︎Show first 5

Misc. 50

  1. 口腔microbiomeとmicrobiotaの現状と今後の課題 口腔細菌叢からacetaldehyde産生、分解菌を探索する(The Current Reports on Oral Microbiome and Microbiota by Promising Challengers Search for Acetaldehyde-producing and -degrading Bacteria from the Oral Microbiome)

    Sato Chika, Tagaino Ryo, Washio Jumpei, Abiko Yuki, Igarashi Kaoru, Takahashi Nobuhiro

    Journal of Oral Biosciences Supplement　2024　[US8-03]　2024/11

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  2. 口腔保健用機能性食品研究会の挑戦 ~口腔保健から、口腔機能を活かした全身の健康へ~ Peer-reviewed

    高橋信博

    デンタルビジョン　25-28　2021/04

  3. Study of all-solid-state ion selective electrodes for multi-ion monitoring in the intraoral

    真柳弦, 鷲尾純平, 高橋信博, 大久保信吾, 山田哲也, 石原昇, 柳田保子

    生体医歯工学共同研究拠点成果報告書　2020　96-96　2021

    Publisher: 東京医科歯科大学生体材料工学研究所

  4. プラークのもつ硝酸還元活性・亜硝酸分解活性と酸産生活性抑制効果との関連

    山本 祐慈, 清水 弘一, 五十嵐 公英, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　69　(増刊)　168-168　2019/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  5. 細菌-材料インターフェイスにおけるフッ素徐放性材料のpH低下抑制効果の評価および将来への展望

    真柳 弦, 鷲尾 純平, 高橋 信博, 柳田 保子, 伊藤 浩之, 石原 昇, 益 一哉

    生体医歯工学共同研究拠点成果報告書　平成29年度　98-98　2018/04

    Publisher: 東京医科歯科大学生体材料工学研究所

  6. プラークのもつ硝酸還元活性と亜硝酸還元活性

    山本 祐慈, 清水 弘一, 五十嵐 公英, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　68　(増刊)　178-178　2018/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  7. 連携機能を活用した歯学教育高度化プログラム

    竹石 龍右, 加来 咲子, 天雲 太一, 冨士 岳志, 三村 純代, 首藤 崇裕, 石田 陽子, 秋葉 奈美, 魚島 勝美, 小野 和宏, 高橋 信博, 二川 浩樹, 加藤 功一, 佐々木 啓一, 前田 健康

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　36回　125-125　2017/07

    Publisher: 日本歯科医学教育学会

  8. 健康長寿を育む歯学教育コンソーシアム 東北大学の取り組み(第3報)

    真柳 弦, 高橋 信博, 笹野 泰之, 福本 敏, 服部 佳功, 佐々木 啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　36回　150-150　2017/07

    Publisher: 日本歯科医学教育学会

  9. 歯学系グローバル人材育成における東北大学の取り組み

    洪 光, 斎藤 恵一, 小坂 健, 高橋 信博, 佐々木 啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　35回　96-96　2016/07

    Publisher: 日本歯科医学教育学会

  10. 連携機能を活用した歯学教育高度化プログラム 平成26〜27年度の実績

    竹石 龍右, 加来 咲子, 天雲 太一, 竹内 裕尚, 三村 純代, 首藤 崇裕, 石田 陽子, 秋葉 奈美, 魚島 勝美, 小野 和宏, 高橋 信博, 二川 浩樹, 佐々木 啓一, 菅井 基行, 前田 健康

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　35回　99-99　2016/07

    Publisher: 日本歯科医学教育学会

  11. 東日本大震災直後に入学した歯学部学生の災害歯科に対する意識調査

    冨士 岳志, 洪 光, 鈴木 敏彦, 小坂 健, 高橋 信博, 佐々木 啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　35回　99-99　2016/07

    Publisher: 日本歯科医学教育学会

  12. 健康長寿を育む歯学教育コンソーシアム 東北大学の取り組み(第2報)

    真柳 弦, 高橋 信博, 笹野 泰之, 福本 敏, 服部 佳功, 佐々木 啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　35回　155-155　2016/07

    Publisher: 日本歯科医学教育学会

  13. 東北大学歯学研究科・歯学部での留学生を対象とした災害歯科学教育

    冨士 岳志, 洪 光, 鈴木 敏彦, 齋藤 恵一, 坪井 明人, 小坂 健, 高橋 信博, 佐々木 啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　34回　138-138　2015/07

    Publisher: 日本歯科医学教育学会

  14. 健康長寿を育む歯学教育コンソーシアム―東北大学の取り組み―

    真柳弦, 高橋信博, 笹野泰之, 福本敏, 服部佳功, 佐々木啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　34th　162-162　2015/07

    Publisher: 日本歯科医学教育学会

  15. Oral microbiota in crevices around dental implants: Profiling of oral biofilm

    Takuichi Sato, Lingyang Tian, Yuki Abiko, Jumpei Washio, Nobuhiro Takahashi

    Interface Oral Health Science 2014: Innovative Research on Biosis-Abiosis Intelligent Interface　45-50　2015/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-55192-8_4 　

  16. Interface oral health science 2014: Innovative research on biosis-abiosis intelligent interface

    Keiichi Sasaki, Suzuki Osamu, Nobuhiro Takahashi

    Interface Oral Health Science 2014: Innovative Research on Biosis-Abiosis Intelligent Interface　1-351　2015/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-55192-8 　

  17. 東北大学歯学部の災害歯科学に対する取り組み

    冨士 岳志, 鈴木 敏彦, 小坂 健, 笹野 泰之, 高橋 信博, 坪井 明人, 佐々木 啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　33回　73-73　2014/07

    Publisher: 日本歯科医学教育学会

  18. 歯学大学院教育グローバル化への東北大学の取り組み

    洪 光, 佐々木 啓一, 高橋 信博, 小坂 健, 斎藤 恵一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　32回　98-98　2013/07

    Publisher: 日本歯科医学教育学会

  19. 口腔バイオフィルムの分子生物学的プロファイリング－メタゲノム解析－ Invited

    佐藤 拓一, 河村 好章, 八巻 惠子, 島内 英俊, 高橋 信博

    歯界展望　(特別号)　190　2013

  20. 蛍光フィルターを利用した、感染根管内細菌の迅速定量検出法 Invited

    庄司 恵, 佐藤 愛美加, 佐藤 拓一, 石田 直子, 八巻 惠子, 高橋 信博

    歯界展望　(特別号)　294　2013

  21. メタボローム解析で見えてきた口腔バイオフィルムの機能 「何がいるか」から「何をしているか」へ

    鷲尾 純平, 眞柳 弦, 小川 珠生, 高橋 信博

    日本歯科医師会雑誌　65　(5)　616-616　2012/08

    Publisher: (公社)日本歯科医師会

    ISSN： 0047-1763

  22. プラークと唾液のメタボローム解析 口腔内でのアミノ酸の利用に注目して

    鈴木 啓佑, 月星 陽介, 渡邊 基博, 小川 珠生, 鷲尾 純平, 高橋 信博

    日本歯科医師会雑誌　65　(5)　686-686　2012/08

    Publisher: (公社)日本歯科医師会

    ISSN： 0047-1763

  23. Silent aspiration of oral bacteria – Microbiological analysis of intraoperative bronchial fluids from patients with pulmonary carcinoma

    Hasegawa A, Sato T, Hoshikawa Y, Kondo T, Takahashi N

    Interface Oral Health Science 2011　181-182　2012/02

  24. Bicarbonate decreases inhibitory effects of fluoride on acid production by Actinomyces naeslundii

    Junko Kawashima, Kazuko Nakajo, Jumpei Washio, Hidetoshi Shimauchi, Nobuhiro Takahashi

    Interface Oral Health Science 2011　189-191　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_51 　

  25. A new method to evaluate pH at the interface between parasites and biomaterials

    Gen Mayanagi, Koei Igarashi, Jumpei Washio, Kazuko Nakajo, Hitomi Domon-Tawaraya, Nobuhiro Takahashi

    Interface Oral Health Science 2011　192-194　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_52 　

  26. Metabolomics of oral plaque biofilm using CE-TOFMS

    Jumpei Washio, Gen Mayanagi, Nobuhiro Takahashi

    Interface Oral Health Science 2011　218-220　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_61 　

  27. Interface oral health science 2011

    Keiichi Sasaki, Osamu Suzuki, Nobuhiro Takahashi, Philip Stashenko, Thomas E. Van Dyke, Toshihisa Kawai, Hatice Hasturk, Alpdogan Kantarci

    Interface Oral Health Science 2011　1-422　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0 　

  28. Change in infected root canal microflora during the course of root canal therapy

    Keiko Yamaki, Takuichi Sato, Ayako Hasegawa, Yuki Abiko, Kazuhiro Hashimoto, Yasuhisa Takeuchi, Junko Matsuyama, Hidetoshi Shimauchi, Nobuhiro Takahashi

    Interface Oral Health Science 2011　181-182　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_62 　

  29. A highly sensitive alamarBlue® method for evaluating bacterial adhesion to biomaterials

    Yoko Sakuma, Jumpei Washio, Yasuhisa Takeuchi, Keiichi Sasaki, Nobuhiro Takahashi

    Interface Oral Health Science 2011　201-203　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0-55 　

  30. Quantification and identification of bacteria in maxillary obturator-prostheses

    Yasuhisa Takeuchi, Kazuko Nakajo, Takuichi Sato, Yoko Sakuma, Shigeto Koyama, Keiichi Sasaki, Nobuhiro Takahashi

    Interface Oral Health Science 2011　209-211　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_58 　

  31. Silent aspiration of oral bacteria in elderly patients

    Ayako Hasegawa, Takuichi Sato, Yasushi Hoshikawa, Takashi Kondo, Nobuhiro Takahashi

    Interface Oral Health Science 2011　181-182　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_48 　

  32. Divalent cations enhance short-time fluoride exposure-induced inhibition on acid production by Streptococcus mutans

    Hitomi Domon-Tawaraya, Kazuko Nakajo, Jumpei Washio, Satoshi Fukumoto, Nobuhiro Takahashi

    Interface Oral Health Science 2011　178-180　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_47 　

  33. Subgingival plaque biofilm microflora of elderly subjects

    Yuki Abiko, Takuichi Sato, Reiko Sakashita, Nobuhiro Takahashi

    Interface Oral Health Science 2011　176-177　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_46 　

  34. Acid tolerance and endogenous acid production by oral bifidobacteria

    Kazuko Nakajo, David Beighton, Nobuhiro Takahashi

    Interface Oral Health Science 2011　195-197　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_53 　

  35. Breath acetone in type 1 and type 2 diabetes mellitus

    Naoko Tanda, Yoshinori Hinokio, Jumpei Washio, Nobuhiro Takahashi, Takeyoshi Koseki

    Interface Oral Health Science 2011　212-214　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_59 　

  36. Interdisciplinary and international research/education based on interface oral health science

    Nobuhiro Takahashi

    Interface Oral Health Science 2011　43-45　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_8 　

  37. Metabolomic approach to oral microbiota

    Nobuhiro Takahashi, Jumpei Washio, Gen Mayanagi

    Interface Oral Health Science 2011　334-340　2012/01/01

    Publisher: Springer Japan

    DOI： 10.1007/978-4-431-54070-0_98 　

  38. ヒト・プラークとう蝕関連口腔細菌Streptococcus mutansの糖代謝に対するキシリトールの影響の相違

    鷲尾 純平, 高橋 信博

    日本生化学会大会プログラム・講演要旨集　84回　4P-0453　2011/09

    Publisher: (公社)日本生化学会

  39. Detection of viable bacterial cells in acrylic resin denture bases

    Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Sasaki K, Takahashi N

    Interface Oral Health Science 2009　230-231　2010

  40. Porphyromonas gingivalis is widely distributed in subgingival plaque biofilm of elderly people

    Abiko Y, Sato T, Matsushita K, Sakashita R, Takahashi N

    Interface Oral Health Science 2009　240-242　2010

  41. Profiling of dental plaque microflora on root caries lesions and the protein-degrading activity of these bacteria

    Hashimoto K, Sato T, Shimauchi H, Takahashi N

    Interface Oral Health Science 2009　243-244　2010

  42. Involvement of cough reflex impairment and silent aspiration of oral bacteria in postoperative pneumonia: A model of aspiration pneumonia

    Sato T, Hoshikawa Y, Kondo T, Hashimoto K, Abiko Y, Hasegawa A, Matsuyama J, Takahashi N

    Interface Oral Health Science 2009　273-274　2010

  43. Profiling of dental plaque biofilm on first molars with orthodontic bands and brackets

    Komori R, Sato T, Takano-Yamamoto T, Takahashi N

    Interface Oral Health Science 2009.　2010

  44. う蝕関連口腔細菌の糖代謝メタボローム解析 糖代謝機能の類似と相違

    鷲尾 純平, 真柳 弦, 高橋 信博

    日本生化学会大会プログラム・講演要旨集　82回　4T15a-2　2009/09

    Publisher: (公社)日本生化学会

  45. CE-TOFMSを用いたプラークバイオフィルムの糖代謝メタボローム解析

    鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　59　(4)　370-370　2009/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  46. 試作Ti‐Ag合金はバイオフィルム形成を抑制する

    高橋正敏, 中條和子, 菊地聖史, 高田雄京, 佐々木啓一, 高橋信博, 奥野攻

    東北大学歯学雑誌　28　(1)　27-27　2009/06/30

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  47. 口腔環境中の乳酸はVeillonellaの硫化水素産生を促進する

    鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　57　(4)　389-389　2007/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  48. 根尖性歯周炎により破壊された歯周組織の治癒のメカニズムの解明と再生医療への応用 16S rRNA遺伝子解析による根尖性歯周炎罹患歯の根管内細菌叢のプロファイリング-感染根管治療前後における細菌叢の変動-

    伊藤康博, 伊藤康博, 佐藤拓一, 真柳弦, 八巻恵子, 高橋信博, 島内英俊

    根尖性歯周炎により破壊された歯周組織の治癒のメカニズムの解明と再生医療への応用 平成16-18年度 No.16209056　2007

  49. 高齢者歯髄の免疫防御機構に関する研究

    大島勇人, 佐藤拓一, 高橋信博, 野村修一, 大島邦子, 監物新一, 川岸恵理子, 楯泰昌

    大和証券ヘルス財団研究業績集　(27)　98-103　2004/03

    Publisher: (公財)大和証券ヘルス財団

  50. 口腔領域の慢性感染症,特に下顎骨骨髄炎及び感染性歯根嚢砲に関する研究

    佐藤拓一, 高橋信博

    大和証券ヘルス財団研究業績集　(26)　10-14　2003/03

Show all ︎Show first 5

Books and Other Publications 30

  1. Oral Microbiome - Symbiosis, dysbiosis and microbiome interventions for maintaining oral and systemic health

    Washio J., Takahashi N.

    Springer Nature　2025/04

  2. 歯科衛生学シリーズ 生化学・口腔生化学 初版

    高橋信博

    医歯薬出版　2025/01

  3. 歯科衛生学シリーズ 歯科予防処置論・歯科保健指導論 第2版

    高橋信博

    医歯薬出版　2024/10

  4. Dental Caries: The Disease and its Clinical Management, 4th Edition

    Takahashi N., Nyvad B.

    Wiley-Blackwell　2024/10

  5. ポリフェノールの機能と多角的応用

    鷲尾純平, 安彦友希, 髙橋信博

    シーエムシー出版　2022/04

  6. 歯科衛生学辞典

    高橋信博

    末永書店　2020/04

  7. シンプル生化学 第7版

    高橋信博

    南江堂　2020/02

  8. 最新歯科衛生士教本用語集

    髙橋信博

    医歯薬出版　2019/04

  9. 最新歯科衛生士教本用語集ポケット版

    高橋 信博

    医歯薬出版　2019/03

  10. 口腔生化学第6版

    高橋 信博

    医歯薬出版　2018/09

  11. 歯科衛生士国家試験問題研究会（編）「歯科衛生士国家試験予想問題集第2版－新出題基準準拠－」

    高橋 信博

    医歯薬出版　2018/03

  12. Interface Oral Health Science 2016: Innovative Research on Biosis-Abiosis Intelligent Interface

    Keiichi Sasaki, Osamu Suzuki, Nobyuhiro Takahashi

    Springer　2017

  13. Dental Caries: The Disease and its Clinical Management, 3rd Edition

    Marsh PD, Takahashi N, Nyvad B

    Wiley-Blackwell　2015/06

  14. Interface Oral Health Science 2014: Innovative Research on Biosis-Abiosis Intelligent Interface

    K. Sasaki, O. Suzuki, N. Takahashi

    Springer　2015

  15. 「デンタルカリエス―その病態と臨床マネージメント 原著第２版（Dental Caries -The Disease and its Clinical Management」翻訳

    高橋信博, 恵比須繁之

    医歯薬出版　2013/10

  16. 歯学英和辞典

    高橋 信博

    研究社　2012

  17. 口腔生化学 第5版

    高橋 信博

    医歯薬出版　2011

  18. Interface Oral Health Science 2009

    T. Sasano, O. Suzuki, P. Stashenko, K. Sasaki, N. Takahashi, T. Kawai, M. A. Tabman, H. C. Margolis, assoc ed

    Springer　2010

  19. 最新歯科衛生士教本 人体の構造と機能2 栄養と代謝

    高橋信博, 佐藤裕, 阿部昌子, 江指隆年, 花井美保, 酒井映子

    医歯薬出版　2010

  20. 歯科衛生士別冊 クイズう蝕＆歯周病の基礎知識100－解いて理解して歯科衛生士臨床パワーアップ－

    高橋 信博

    クインテッセンス　2009

  21. う蝕学－チェアサイドの予防と回復のプログラム－

    高橋 信博

    2008

  22. Interface Oral Health Science 2007

    Makoto Watanabe, Isamu, Okuno Keiichi, Sasaki Osamu, Suzuki Nobuhiro, Takahashi ed

    Springer　2007/12

  23. シンプル生化学

    高橋 信博

    南江堂　2007

  24. 新・う蝕の科学

    浜田茂幸, 大嶋隆, ed, 高橋信博

    2006/05

  25. 口腔生化学（第4版）

    木崎治俊, 畑隆一郎, 高橋信博, 宇田川信之

    医歯薬出版　2005/04

  26. Congress series 1284: International Symposium for Interface Oral Health Science

    Makoto Watanabe, Nobuhiro Takahashi Haruhiko, Takada ed

    Elsevier　2005/04

  27. 分子生物学歯科小事典

    西澤俊樹, 花田信弘, 今井奨, 西原達次, 高橋信博

    口腔保健協会　2003/04

  28. 一般医化学改訂（第7版）

    菊地吾郎, 立木蔚, 山田正, 柴原茂樹, 田村眞理, 高橋信博

    南山堂　2002/04

  29. 先端医療シリーズ・歯科医学２ 歯周病－新しい治療を求めて

    高橋 信博

    先端医療技術研究所　2000

  30. Flavins and flavoproteins

    Higuchi M, Yamamoto Y, Poole L, Shimada M, Sato Y, Takahashi N, Kamio Y

    Rudolf Weber/Agency for Scientific Publications　1999

Show all Show first 5

Presentations 169

  1. 口腔microbiomeとmicrobiotaの現状と今後の課題 口腔細菌叢からacetaldehyde産生、分解菌を探索する(The Current Reports on Oral Microbiome and Microbiota by Promising Challengers Search for Acetaldehyde-producing and -degrading Bacteria from the Oral Microbiome)

    Sato Chika, Tagaino Ryo, Washio Jumpei, Abiko Yuki, Igarashi Kaoru, Takahashi Nobuhiro

    Journal of Oral Biosciences Supplement　2024/11

  2. 口腔Neisseria及び口腔Veillonellaによる口腔マイクロバイオーム内のpH低下に対する抑制効果

    鷲尾 純平, 江副 和子, 佐藤 聡子, 安彦 友希, 高橋 信博

    Journal of Oral Biosciences Supplement　2024/11

  3. 緑茶カテキンEGCGによる歯周病関連細菌に対する抗菌効果,およびう蝕関連細菌に対する効果との比較

    樋口 真由, 安彦 友希, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　2024/04

  4. Development of micro-device to measure metabolic-related markers with ultra-high sensitivity

    鷲尾純平, 高橋信博, 安彦友希, 米田将太, 柳田保子

    生体医歯工学共同研究拠点成果報告書　2024

  5. 細菌性代謝産物の酪酸が歯肉線維芽細胞の機能に与える影響

    大谷 栄毅, 鷲尾 純平, 國富 葵, 佐々木 詩織, 大橋 一正, 山田 聡, 高橋 信博

    日本歯周病学会会誌　2023/10

  6. 口腔Actinomyces属及びSchaalia属の亜硝酸塩産生活性と活性に対する環境要因の影響

    大竹 知菜, 鷲尾 純平, 安彦 友希, 五十嵐 薫, 高橋 信博

    Journal of Oral Biosciences Supplement　2023/09

  7. 歯周病関連細菌に由来する酪酸が歯周組織細胞機能に与える影響

    大谷 栄毅, 鷲尾 純平, 佐藤 聡子, 佐々木 詩織, 山田 聡, 高橋 信博

    Journal of Oral Biosciences Supplement　2023/09

  8. 静脈麻酔薬プロポフォールが肝細胞の糖代謝活性に及ぼす影響

    佐々木 詩織, 鷲尾 純平, 大谷 栄毅, 佐藤 聡子, 水田 健太郎, 高橋 信博

    Journal of Oral Biosciences Supplement　2023/09

  9. 口腔マイクロバイオームは発がん性物質アセトアルデヒドの分解にも関与する アセトアルデヒド産生能・分解能の簡易スクリーニング法の確立

    佐藤 知佳, 互野 亮, 鷲尾 純平, 安彦 友希, 五十嵐 薫, 高橋 信博

    Journal of Oral Biosciences Supplement　2023/09

  10. 歯科訪問診療における水質管理方策の検討

    伏見 国拡, 山田 将博, 渡辺 隼, 鷲尾 純平, 高橋 信博, 江草 宏

    東北大学歯学雑誌　2023/06

  11. 緑茶カテキンの歯周病関連細菌に対する抗菌作用 アミノ酸代謝活性抑制および菌体凝集作用について

    樋口 真由, 安彦 友希, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　2023/04

  12. 唾液組成のモニタリングに向けた全固体型イオン選択電極のマイクロ流路への統合

    真柳 弦, 鷲尾 純平, 高橋 信博, 大関 祥久, 山田 哲也, 石原 昇, 柳田 保子

    生体医歯工学共同研究拠点成果報告書　2023/04

  13. 搾乳母乳と新生児口腔内細菌叢との連関

    涌井 杏奈, 河内 美帆, 佐野 拓人, 坂下 玲子, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    口腔衛生学会雑誌　2023/04

  14. 口腔マイクロバイオームは発がん性物質アセトアルデヒドの分解にも関与する。-アセトアルデヒド産生能・分解能の簡易スクリーニング法の確立-

    佐藤知佳, 佐藤知佳, 互野亮, 互野亮, 鷲尾純平, 安彦友希, 五十嵐薫, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2023

  15. Scardovia wiggsiaeの増殖促進因子の検討(Growth promoting factors of Scardovia wiggsiae)

    亀田 真衣, 安彦 友希, 鷲尾 純平, 佐藤 聡子, 高橋 信博

    Journal of Oral Biosciences Supplement　2022/09

  16. 口腔内マルチイオン計測に向けた全固体イオン選択電極の開発

    真柳 弦, 鷲尾 純平, 高橋 信博, 大関 祥久, 山田 哲也, 大久保 信吾, 齊藤 滉佑, 石原 昇, 柳田 保子

    生体医歯工学共同研究拠点成果報告書　2022/03

  17. Metabolic functions of the oral microbiome: How does the oral microbiome function and contribute to our health? Invited

    Washio J, Mayanagi G, Abiko Y, Tagaino R, Dimas Prasetianto Wicaksono, Domon H, Ezoe K, Sato S, Takahashi N

    International Symposium for Interface Oral Health Science 2022 (IOHS)　2022/01/15

  18. Shoku-gaku: Transdisciplinary Science of Eating, Food, and Nutrition – Promoting lifelong health and wellbeing through right eating and right food – Invited

    Takahashi N

    International Symposium for Interface Oral Health Science 2022 (IOHS)　2022/01/15

  19. Growth promoting factors of Scardovia wiggsiae

    亀田真衣, 亀田真衣, 安彦友希, 鷲尾純平, 佐藤聡子, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2022

  20. Integration of all-solid-state electrode into the microfluidic channel

    大関祥久, 山田哲也, 石原昇, 柳田保子, 真柳弦, 真柳弦, 鷲尾純平, 高橋信博

    精密工学会大会学術講演会講演論文集　2022

  21. Unique acid production from sugars and fluoride resistance of a novel caries-associated bacterium Scardovia wiggsiae Invited

    Kameda M, Abiko Y, Washio J, Takahashi N

    2021FJMU-HKU-TU International Online Symposium on Oral Health Sciences　2021/11/18

  22. Profiling of microbiota in various drinks of plastic bottle after drinking directly from plastic bottle. Invited

    Miho Kawachi, Nanase Takahashi, Nagara Kaku, Mayu Higuchi, Anna Wakui, Jumpei Washio, Nobuhiro Takahashi, Takuichi Sato

    2021/10/09

  23. The study on the production of acetaldehyde from ethanol by oral bacteria - Effects of oral environmental factors and ethanol concentration Invited

    Ryo TAGAINO, Jumpei WASHIO, Keiichi SASAKI, Nobuhiro TAKAHASHI

    2021/10/09

  24. 若手研究者による口腔微生物叢に関するチャレンジレポート 口腔内細菌によるエタノールからのアセトアルデヒド産生に関する研究 口腔内環境因子の影響とエタノール濃度(The Challenge Reports on Oral Microbiota by Young Researchers The study on the production of acetaldehyde from ethanol by oral bacteria: Effects of oral environmental factors and ethanol concentration)

    Tagaino R, Washio J, Sasaki K, Takahashi N

    Journal of Oral Biosciences Supplement　2021/10

  25. 根面う蝕モデル 酸による根面内在性タンパク分解酵素の活性化(Root caries model: Acid-induced activation of endogenous proteases embedded in root surfaces)

    櫻井 泉, 真柳 弦, 山田 聡, 高橋 信博

    Journal of Oral Biosciences Supplement　2021/10

  26. 口腔Neisseria属による亜硝酸産生活性と環境因子による影響(The Nitrite producing activity of oral Neisseria and the effect of environmental factors)

    鷲尾 純平, 江副 和子, 佐藤 聡子, 安彦 友希, 小峰 英也, 柴田 怜, 田花 航平, 毛 雪竹, 高橋 信博

    Journal of Oral Biosciences Supplement　2021/10

  27. Cariogenic potential of a novel caries-associated bacterium Scardovia wiggsiae, based on its unique carbohydrate metabolism Invited

    Kameda M, Abiko Y, Washio J, Takahashi N

    The 16th International Workshop on Biomaterials in Interface Science　2021/09/21

  28. 小児齲蝕の発症機序と 生物学的要因 Invited

    高橋信博

    第70回日本口腔衛生学会 ミニシンポジウム　2021/05/27

  29. ⼝腔と全⾝の健康に関わる ⼝腔バイオフィルムの代謝機能 Invited

    高橋信博, 鷲尾純平

    第94回⽇本細菌学会 【シンポジウム5】集団微⽣物学と細菌バイオフィルム研究の前線　2021/03/24

  30. Oral microbiome metabolism in oral and systemic health/disease Invited

    Takahashi N

    International Mini-Symposium of the 13th Research Day　2021/03/19

  31. ;飲みかけのペットボトル飲料物(お茶;スポーツ飲料;コーラ;オレンジジュース;および飲み口から検出された細菌の量;構成の解析

    河内美帆, 丸山伸吾, 枡田菜々子, 涌井杏奈, 佐野拓人, 鷲尾純平, 安彦友希, 石黒和子, 真柳 弦, 高橋信博, 佐藤拓一

    第10回口腔保健用機能性食品研究会　2021/02/20

  32. 集団微生物学と細菌バイオフィルム研究の前線 口腔と全身の健康に関わる口腔バイオフィルムの代謝機能(Metabolic function of oral biofilm involved in oral and systemic health)

    高橋 信博, 鷲尾 純平

    日本細菌学雑誌　2021/02

  33. The Nitrite producing activity of oral Neisseria and the effect of environmental factors

    鷲尾純平, 江副和子, 佐藤聡子, 安彦友希, 小峰英也, 柴田怜, 田花航平, MAO X, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2021

  34. Root caries model: Acid-induced activation of endogenous proteases embedded in root surfaces

    櫻井泉, 櫻井泉, 真柳弦, 真柳弦, 山田聡, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2021

  35. Study of all-solid-state ion selective electrodes for multi-ion monitoring in the intraoral

    真柳弦, 鷲尾純平, 高橋信博, 大久保信吾, 山田哲也, 石原昇, 柳田保子

    生体医歯工学共同研究拠点成果報告書　2021

  36. 私たちの考える「食学」とは Invited

    高橋信博

    『食学拠点の形成』キックオフ・シンポジウム　2020/12/21

  37. 「口腔マイクロバイオームの細菌叢・代謝解析を基盤とした「食からのヘルスプロモーション」」 Invited

    鷲尾純平, 互野亮, 真柳弦, 安彦友希, 江副和子, Dimas Prasetianto Wicaksono, 韓思理, 大竹知菜, 柳田保子, 髙橋信博

    『食学拠点の形成』キックオフシンポジウム　2020/12/21

  38. Metformin inhibits oral cancer cell growth by altering glucose metabolism.

    Liu S, Washio J, Takahashi N

    The 68th Annual meeting of Japanese Association for Dental Research　2020/11/07

  39. Involvement of oral bacterial metabolism in oral health and diseases- Importance of analyzing bacterial metabolic activity Invited

    Washio J, Takahashi N

    The 2nd International Cooperation Symposium　2020/10/15

  40. Involvement of oral bacterial metabolism in oral health and diseases- Importance of analyzing bacterial metabolic activity, Invited

    Washio J, Takahashi N

    The 2nd International Cooperation Symposium　2020/10/15

  41. Metabolism of Oral Microbiome: From Its Fundamentals to the Latest Metabolome Analysis Invited

    Takahashi N

    Dental Medicine Doctoral Program (Research Based)　2020/10/10

  42. 飲酒を想定した中から高濃度エタノール環境下におけるStreptococcus属によるアセトアルデヒド産生

    互野 亮, 鷲尾 純平, 佐々木 啓一, 高橋 信博

    第62回歯科基礎医学会学術大会　2020/09/11

  43. 口腔内細菌叢解析システム;PCR-RFLP法によるプロファイリング

    河内 美帆, 丸山 伸吾, 枡田 菜々子, 佐野 拓人, 涌井 杏奈, 八巻 惠子, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    第62回歯科基礎医学会学術大会　2020/09/11

  44. Effect of nitrate;nitrite;on the growth;N;roducing activity of oral Veillonella species

    Wicaksono D. P, Washio J, Takahashi N

    2020/09/11

  45. The antimicrobial;properties of green;tea-derived epigallocatechin gallate on;Streptococcus mutans

    Han S, Abiko Y, Washio J, Luo Y, Zhang L, Takahashi N

    2020/09/11

  46. Profiling of Microbiota of Baby-Drinks after Drinking with Artificial Nipples

    Wakui A, Sano H, Kawachi M, Masuda N, Maruyama S, Washio J, Abiko Y, Mayanagi G, Yamaki K, Takahashi N, Okada Y, Sato T

    2020/09/11

  47. 口腔内環境モニタリングに向けた全固体イオン選択電極センサアレイの開発と多変量解析アルゴリズムの検討．

    大久保信吾, 石原昇, 山田哲也, 栁田保子, 真柳弦, 鷲尾純平, 高橋信博

    日本化学会第15回バイオ関連化学シンポジウム　2020/09/08

  48. Metformin Influenced Glucose Metabolic Activity of Cancer Cells.

    Kobayashi Y, Washio J, Shinohara Y, Takahshi N

    The 98h IADR General Session & Exhibition,　2020/03/01

  49. ニプル（哺乳瓶用乳首）を通した、口腔から液体ミルク内への流入

    涌井杏奈, 佐野拓人, 河内美帆, 鷲尾純平, 安彦友希, 真柳 弦, 高橋信博, 佐藤拓一

    第9回口腔保健用機能性食品研究会　2020/02/01

  50. Green tea-derived epigallocatechin gallate reduced the acid production and promoted the aggregation of Streptococcus mutans.

    Han S, Abiko Y, Washio J, Luo Y, Zhang L, Takahashi N

    2020/02/01

  51. 母乳および新生児の口腔内の細菌叢解析：保管による影響について

    佐野拓人, 涌井杏奈, 河内美帆, 森山幸枝, 西方真弓, 鷲尾純平, 安彦友希, 真柳 弦, 高橋信博, 佐藤拓一

    第9回口腔保健用機能性食品研究会　2020/02/01

  52. Fabrication of calcium ion selective electrode for intraoral multi ion measurement microdevice

    真柳弦, 高橋信博, 齊藤滉佑, 石原昇, 柳田保子

    生体医歯工学共同研究拠点成果報告書　2020

  53. Profiling of Microbiota of Liquid Baby Formula after Drinking with Artificial Nipples

    Wakui A, Sano H, Kawachi M, Kato R, Moriyama S, Nishikata A, Washio J, Yamaki K, Sakashita R, Sato T

    4th Asia Pacific Regional Congress of the International Association for Dental Research　2019/11/01

  54. Profiling System of Oral Biofilm Microbiota Utilizing;PCR-RFLP Analysis

    Sano H, Wakui A, Washio J, Yamaki K, Sato T

    4th Asia Pacific Regional Congress of the International Association for Dental Research　2019/11/01

  55. (Green tea-derived epigallocatechin gallate inhibited the acid production of Streptococcus mutans and promoted the bacterial aggregation)

    2019/10/01

  56. 温熱による癌細胞の糖代謝活性への影響

    小林 ゆり, 鷲尾 純平, 篠原 優太, 高橋 信博

    第61回歯科基礎医学会学術大会　2019/10/01

  57. PCR-RFLP法を用いた口腔内細菌叢解析(プロファイリング)システム

    佐野 拓人, 涌井 杏奈, 八巻 惠子, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    第61回歯科基礎医学会学術大会　2019/10/01

  58. フッ化物による歯周病関連菌Porphyromonas gingivalisの増殖・代謝の抑制効果

    土門 ひと美, 鷲尾 純平, 安彦 友希, 川嶋 順子, 高橋 信博

    第61回歯科基礎医学会学術大会　2019/10/01

  59. The Challenge Reports of Oral Biofilm by Young Researchers Profiling of microbiota in baby-drinks and liquid baby formula consumed with an artificial nipple

    Wakui A, Sano H, Kawachi M, Kato R, Washio J, Abiko Y, Mayanagi G, Yamaki K, Takahashi N, Sato T

    2019/10/01

  60. Which oral environmental factors enhance acetaldehyde production by oral streptococci?

    Tagaino R, Washio J, Abiko Y, Tanda N, Sasaki K, Takahashi N

    The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development (iLIM-4)　2019/10/01

  61. Nitrate induced;the nitrite;producing activity of oral Veillonella -A possible role of nitrate;oral Veilonella on oral;general health

    Dimas Prasetianto Wicaksono, Washio J, Takahashi N

    The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development (iLIM-4)　2019/10/01

  62. Nitrate;nitrite;promote the growth;nitrite;roduction of oral Veillonella

    Dimas Prasetianto Wicaksono, Washio J, Takahashi N

    2019/09/01

  63. Environmental Nitrate/nitrite Accelerated Growth and Increased Nitrite-production of Oral Veillonella

    Dimas Prasetianto Wicaksono, Washio J, Takahashi N

    The 97h IADR General Session & Exhibition　2019/06/01

  64. Physiological properties of acetaldehyde production from ethanol by oral Streptococcus.

    Tagaino R, Washio J, Abiko Y, Tanda N, Sasaki K, Takahashi N

    The 97h IADR General Session & Exhibition　2019/06/01

  65. プラークのもつ硝酸還元活性・亜硝酸分解活性と酸産生活性抑制効果との関連

    山本 祐慈, 清水 弘一, 五十嵐 公英, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　2019/04

  66. A novel method to screen and isolate nitrite-producing bacteria from the oral cavity

    Washio J, Suzuki Y, Sato T, Akiba M, Igarashi A, Kimura S, Tezuka S, Nakazato K, Takahashi N

    A novel method to screen and isolate nitrite-producing bacteria from the oral cavity International Symposium for Multimodal research and Education in IOHS-Liaison 2019　2019/01/11

  67. 口腔内微小環境マルチイオン測定マイクロデバイスのためのイオン選択膜の作製

    真柳弦, 高橋信博, 齊藤滉佑, 石原昇, 柳田保子

    生体医歯工学共同研究拠点成果報告会　2019

  68. Diamond-like carbonのバイオフィルム付着抑制効果

    石幡浩志, 真柳弦, 佐藤秀明, 吉川亮太, 藤本佑典, 高橋信博, 山田聡

    砥粒加工学会学術講演会講演論文集(CD-ROM)　2019

  69. 健康長寿を育む歯学教育コンソーシアム-東北大学の5年間の取り組みとこれから-

    真柳弦, 真柳弦, 高橋信博, 高橋信博, 笹野泰之, 笹野泰之, 福本敏, 福本敏, 服部佳功, 服部佳功, 佐々木啓一, 佐々木啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2019

  70. 健康長寿を育む歯学教育コンソーシアム―東北大学の取り組み 第4報―

    真柳弦, 高橋信博, 笹野泰之, 福本敏, 服部佳功, 佐々木啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2018/07

  71. プラークのもつ硝酸還元活性と亜硝酸還元活性

    山本 祐慈, 清水 弘一, 五十嵐 公英, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　2018/04

  72. 細菌-材料インターフェイスにおけるフッ素徐放性材料のpH低下抑制効果の評価および将来への展望

    真柳 弦, 鷲尾 純平, 高橋 信博, 柳田 保子, 伊藤 浩之, 石原 昇, 益 一哉

    生体医歯工学共同研究拠点成果報告書　2018/04

  73. ニプルを通して飲んだ際の,ベビー飲料内への口腔細菌の流入

    相原瞳, 涌井杏奈, 佐野拓人, 河内美帆, 鷲尾純平, 安彦友希, 真柳弦, 八巻恵子, 高橋信博, 佐藤拓一

    Journal of Oral Biosciences Supplement (Web)　2018

  74. 連携機能を活用した歯学教育高度化プログラム

    竹石 龍右, 加来 咲子, 天雲 太一, 冨士 岳志, 三村 純代, 首藤 崇裕, 石田 陽子, 秋葉 奈美, 魚島 勝美, 小野 和宏, 高橋 信博, 二川 浩樹, 加藤 功一, 佐々木 啓一, 前田 健康

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2017/07

  75. 健康長寿を育む歯学教育コンソーシアム―東北大学の取り組み 第3報―

    真柳弦, 高橋信博, 笹野泰之, 福本敏, 服部佳功, 佐々木啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2017/07

  76. 飲料物の飲み口に付着する細菌の量および構成の解析

    佐野拓人, 平吹有香, 涌井杏奈, 曾田彩花, 安彦友希, 八巻恵子, 真柳弦, 鷲尾純平, 高橋信博, 佐藤拓一

    Journal of Oral Biosciences Supplement (Web)　2017

  77. 長期使用義歯からの細菌由来揮発性代謝産物の検出

    互野 亮, 末永 華子, 鷲尾 純平, 佐々木 啓一, 高橋 信博

    Journal of Oral Biosciences Supplement　2016/09

  78. 連携機能を活用した歯学教育高度化プログラム 平成26〜27年度の実績

    竹石 龍右, 加来 咲子, 天雲 太一, 竹内 裕尚, 三村 純代, 首藤 崇裕, 石田 陽子, 秋葉 奈美, 魚島 勝美, 小野 和宏, 高橋 信博, 二川 浩樹, 佐々木 啓一, 菅井 基行, 前田 健康

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2016/07

  79. 健康長寿を育む歯学教育コンソーシアム―東北大学の取り組み 第2報―

    真柳弦, 高橋信博, 笹野泰之, 福本敏, 服部佳功, 佐々木啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2016/07

  80. 歯学系グローバル人材育成における東北大学の取り組み

    洪 光, 斎藤 恵一, 小坂 健, 高橋 信博, 佐々木 啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2016/07

  81. 連携機能を活用した歯学教育高度化プログラム―平成26~27年度の実績―

    竹石龍右, 加来咲子, 天雲太一, 竹内裕尚, 三村純代, 首藤崇裕, 石田陽子, 秋葉奈美, 魚島勝美, 小野和宏, 高橋信博, 二川浩樹, 佐々木啓一, 菅井基行, 前田健康

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2016/07

  82. 東日本大震災直後に入学した歯学部学生の災害歯科に対する意識調査

    冨士 岳志, 洪 光, 鈴木 敏彦, 小坂 健, 高橋 信博, 佐々木 啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2016/07

  83. 齲蝕関連Bifidobacteriumの酸産生活性とそのフッ化物による抑制効果

    馬目歩実, 安彦友希, 安彦友希, 川嶋順子, 福本敏, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2016

  84. 窒素ドープ酸化チタンを形成させたチタンのアパタイト形成能と可視光下での抗菌性

    川下将一, 遠藤真子, 渡邉友亮, 宮崎敏樹, 古谷真衣子, 横田琴音, 安彦友希, 金高弘恭, 高橋信博

    日本バイオマテリアル学会大会予稿集　2015/11/09

  85. 健康長寿を育む歯学教育コンソーシアム―東北大学の取り組み―

    真柳弦, 高橋信博, 笹野泰之, 福本敏, 服部佳功, 佐々木啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2015/07

  86. 東北大学歯学研究科・歯学部での留学生を対象とした災害歯科学教育

    冨士 岳志, 洪 光, 鈴木 敏彦, 齋藤 恵一, 坪井 明人, 小坂 健, 高橋 信博, 佐々木 啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2015/07

  87. 東北大学歯学部の災害歯科学に対する取り組み

    冨士 岳志, 鈴木 敏彦, 小坂 健, 笹野 泰之, 高橋 信博, 坪井 明人, 佐々木 啓一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2014/07

  88. 歯学大学院教育グローバル化への東北大学の取り組み

    洪 光, 佐々木 啓一, 高橋 信博, 小坂 健, 斎藤 恵一

    日本歯科医学教育学会総会・学術大会プログラム・抄録集　2013/07

  89. 離乳前後および成熟マウスの口腔内プラーク常在菌叢の網羅的解析

    松山順子, 佐藤拓一, QUISPE-SALCEDO Angela, 高橋信博, 大島勇人

    Journal of Oral Biosciences Supplement (Web)　2013

  90. マウス口腔内プラーク常在菌叢の網羅的解析

    松山順子, 佐藤拓一, ANGELA Quispe-Salcedo, 石田直子, 石田直子, 高橋信博, 大島勇人

    Journal of Oral Biosciences Supplement (Web)　2012

  91. ヒト・プラークとう蝕関連口腔細菌Streptococcus mutansの糖代謝に対するキシリトールの影響の相違

    鷲尾 純平, 高橋 信博

    日本生化学会大会プログラム・講演要旨集　2011/09

  92. ヒト・プラークの糖代謝に対するフッ化物およびキシリトールの影響 CE-TOFMSを用いたメタボロミクス・アプローチ

    鷲尾 純平, 高橋 信博

    Journal of Oral Biosciences　2011/09

  93. 高齢者の歯肉縁下プラーク細菌叢 Porphyromonas gingivalisの定量解析とfimA遺伝子型タイピング

    安彦 友希, 佐藤 拓一, 坂下 玲子, 高橋 信博

    Journal of Oral Biosciences　2011/09

  94. 口腔フローラのプロファイリング

    佐藤 拓一, 安彦 友希, 長谷川 彩子, 真柳 弦, 鷲尾 純平, 中條 和子, 堀 はるみ[宮澤], 高橋 信博

    東北大学歯学雑誌　2011/06

  95. 根面齲触関連菌Actinomycesの酸産生活性とそれに及ぼす環境pH、重炭酸、フッ化物の影響

    川嶋 順子, 中條 和子, 鷲尾 純平, 島内 英俊, 高橋 信博

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集　2011/05

  96. 口腔環境を考慮した糖負荷試験時呼気中アセトン測定と血中ケトン体との関連

    丹田 奈緒子, 檜尾 好徳, 由浪 有希子, 鷲尾 純平, 高橋 信博, 小関 健由

    糖尿病　2011/04

  97. 高齢者の歯肉縁下プラーク細菌叢:Porphyromonas gingivalisの定量解析とfimA遺伝子型タイピング

    安彦友希, 佐藤拓一, 坂下玲子, 高橋信博

    Journal of Oral Biosciences　2011

  98. プラークバイオフィルムのメタボローム解析 糖代謝からアミノ酸代謝まで

    鷲尾 純平, 真柳 弦, 高橋 信博

    Journal of Oral Biosciences　2010/09

  99. 短時間フッ化物曝露によるStreptococcus mutans酸産生抑制効果に与える二価金属イオンの影響

    土門 ひと美[俵谷], 中條 和子, 鷲尾 純平, 福本 敏, 高橋 信博

    Journal of Oral Biosciences　2010/09

  100. 入院中に口腔環境改善を試みた治療コンプライアンスの低い2型糖尿病の1例

    丹田 奈緒子, 檜尾 好徳, 由浪 有希子, 鷲尾 純平, 高橋 信博, 小関 健由, 片桐 秀樹, 岡 芳知

    糖尿病　2010/07

  101. う蝕関連口腔細菌の糖代謝メタボローム解析 糖代謝機能の類似と相違

    鷲尾 純平, 真柳 弦, 高橋 信博

    日本生化学会大会プログラム・講演要旨集　2009/09

  102. CE-TOFMSを用いたプラークバイオフィルムの糖代謝メタボローム解析

    鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　2009/08

  103. 短時間フッ化物曝露によるStreptococcus mutansのフッ素吸着及び酸産生能への影響

    土門 ひと美[俵谷], 中條 和子, 鷲尾 純平, 宮澤 はるみ[堀], 福本 敏, 高橋 信博

    Journal of Oral Biosciences　2009/08

  104. Actinomycesの糖代謝 フッ化物耐性とその重炭酸による影響

    川嶋 順子, 中條 和子, 鷲尾 純平, 島内 英俊, 高橋 信博

    Journal of Oral Biosciences　2009/08

  105. CE-TOFMSを用いたStreptococcus mutansおよびActinomyces naeslundiiの糖代謝メタボローム解析

    鷲尾 純平, 真柳 弦, 高橋 信博

    Journal of Oral Biosciences　2009/08

  106. 高齢者の歯肉縁下プラーク細菌叢へのPorphyromonas gingivalisの定着

    安彦 友希, 佐藤 拓一, 坂下 玲子, 高橋 信博

    Journal of Oral Biosciences　2009/08

  107. 試作Ti‐Ag合金はバイオフィルム形成を抑制する

    高橋正敏, 中條和子, 菊地聖史, 高田雄京, 佐々木啓一, 高橋信博, 奥野攻

    東北大学歯学雑誌　2009/06/30

  108. 歯内治療に伴う,感染根管内細菌叢の変動に関する研究

    佐藤拓一, 八巻惠子, 橋本紀洋, 竹内裕尚, 安彦友希, 島内英俊, 高橋信博

    Journal of Oral Biosciences　2009

  109. Inhibitory action of Ti-Ag alloys on biofilm formation

    Takahashi Masatoshi, Nakajo Kazuko, Kikuchi Masafumi, Takahashi Nobuhiro, Takada Yukyo

    Abstracts of the General Sessions of the Japanese Society for Dental Materials and Devices　2009

    More details Close

    Plates of experimental Ti-Ag alloys, pure titanium, pure silver, and an Au-Ag-Pd alloy were prepared. Streptococcus mutans was cultured anaerobically in a complex culture medium containing 0.5% sucrose in the presence of each plate sterilized. The amounts of biofilm formed on the plates were estimated by optical density. In addition, the bactericidal activity of these plates was evaluated.

  110. バイオマテリアル表面への各種口腔細菌付着量の評価法

    佐久間 陽子, 鷲尾 純平, 竹内 裕尚, 佐々木 啓一, 高橋 信博

    Journal of Oral Biosciences　2008/09

  111. Metabolic Diversity in an Ecological Niche and Relationships to Oral Disease International-presentation

    Nobuhiro Takahashi

    第55回JADR　2007/11/17

  112. う蝕リスク別乳幼児プラーク酸・アルカリ酸性モデル曲線の作製とそれを用いたう蝕リスク評価

    清水弘一, 五十嵐公英, 高橋信博

    第56回日本口腔衛生学会　2007/10/04

  113. 口腔環境中の乳酸はVeillonellaの硫化水素産生を促進する

    鷲尾純平, 高橋信博

    第56回日本口腔衛生学会　2007/10/03

  114. Detection of bacteria from within acrylic resin denture bases International-presentation

    Y. Takeuchi, K. Nakajo, Y. Sakuma, T. Sato, K. Sasaki, N. Takahashi

    12th Meeting of the International College of Prosthodontists　2007/09/06

  115. 唾液ゲラチナーゼ活性とその活性化について.

    三好慶忠, 渡辺誠, 高橋信博

    第49回歯科基礎医学会学術大会　2007/08/30

  116. 高齢者の歯肉縁下プラークバイオフィルムにおける歯周病関連菌の存在

    安彦友希, 佐藤拓一, 松下健二, 高橋信博

    第49回歯科基礎医学会学術大会　2007/08/30

  117. 根面う蝕プラークバイオフィルム細菌叢のプロファイリング

    橋本紀洋, 佐藤拓一, 島内英俊, 高橋信博

    第49回歯科基礎医学会学術大会　2007/08/30

  118. 口腔レンサ球菌によるデンプンからの酸産生とアミラーゼ阻害剤による影響

    相澤志津子, 宮澤?堀はるみ, 真柳秀昭, 高橋信博

    第49回歯科基礎医学会学術大会　2007/08/30

  119. 高濃度フッ化物の口腔内細菌に対する生存抑制効果

    中條和子, 川嶋順子, 会場理恵, 木村茉莉恵, 佐々城真, 高橋信博

    第49回歯科基礎医学会学術大会　2007/08/30

  120. 口腔Veillonellaはcystathionine β-synthase様活性を持ちシステインから硫化水素を産生する.

    鷲尾純平, 高橋信博

    第49回歯科基礎医学会学術大会　2007/08/30

  121. レジン床義歯内部に潜む細菌の検出

    竹内裕尚, 中條和子, 佐藤拓一, 佐々木啓一, 高橋信博

    第49回歯科基礎医学会学術大会　2007/08/30

  122. 舌苔細菌と口臭 〜舌苔常在細菌の口臭産生への関与〜

    鷲尾純平, 佐藤拓一, 中條和子, 高橋信博

    第48回歯科基礎医学会学術大会 サテライトシンポジウム「オーラルフローラ研究の潮流」　2007/08/29

  123. Effects of pH and lactate on the production of hydrogen sulfide by oral Veillonella. International-presentation

    J. Washio, Y. Shimada, R. Sakamaoto, M. Yamada, N. Takahashi

    7th International Conference of the International Society for Breath Odor Research　2007/08/22

  124. 口腔環境中の乳酸はVeillonellaの硫化水素産生を促進する

    鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　2007/08

  125. 口腔細菌のデンプンからの酸産生とそれに対するアミラーゼ阻害剤の効果

    相澤志津子, 宮澤-堀はるみ, 真柳秀昭, 高橋信博

    第45回日本小児歯科学会　2007/07/19

  126. 東北大学大学院歯学研究科社会貢献委員会東北地方の地域歯科保健推進へ向けた東北大学の取り組み 東北大学地域歯科保健推進室の設置.

    小関健由, 小坂健, 高橋信博, 佐々木啓一, 高田春比古, 菊地正嘉, 川村仁, 渡邉誠

    日本口腔衛生学会北日本支部会　2007/07/14

  127. 口腔細菌のデンプンからの酸産生とそれに対するアミラーゼ阻害剤の効果

    相澤 志津子, 宮澤 堀 はるみ, 真柳 秀昭, 高橋 信博

    小児歯科学雑誌　2007/05/25

  128. The Hydrogen sulfide production by Oral Veillonella: effects of substrate and environmental pH. International-presentation

    J. Washio, S. Matoba, T. Seki, N. Yamamoto, M. Yamamoto, N. Takahashi

    The 2nd International Symposium for Interface Oral Health Science in Sendai　2007/02/18

  129. Gelatinase activity in human saliva and its fluctuation in the oral cavity International-presentation

    Y. Miyoshi, M.Watanabe, N.Takahashi

    The 2nd International Symposium for Interface Oral Health Science in Sendai　2007/02/18

  130. Profiling of Subgingival Plaque Biofilm Microflora of Healthy and Periodontitis International-presentation

    Yuki Abiko, Takuichi Sato, Gen Mayanagi, Nobuhiro Takahashi

    The 2nd International Symposium for Interface Oral Health Science in Sendai　2007/02/18

  131. Microflora Profiling of Root Canal Utilizing Real-Time PCR and Cloning-Sequence Analyses Based on 16S rRNA Genes -Differences between Before and After Root Canal Treatments. International-presentation

    Yasuhiro Ito, Takuichi Sato, Gen Mayanagi, Keiko Yamaki, Hidetoshi Shimauchi, Nobuhiro Takahashi

    The 2nd International Symposium for Interface Oral Health Science in Sendai　2007/02/18

  132. Fluoride ion released from glass-ionomer cement is responsible to inhibit the acid production of caries-related oral streptococci. International-presentation

    Kazuko Nakajo, Yusuke Takahashi, Wakako Kiba, Satoshi Imazato, Nobuhiro Takahashi

    The 2nd International Symposium for Interface Oral Health Science in Sendai　2007/02/18

  133. Candida Species as Members of Oral Microflora in Oral Lichen Planus. International-presentation

    Mika Masaki, Takuichi Sato, Yumiko Sugawara, Takashi Sasano, Nobuhiro Takahashi

    The 2nd International Symposium for Interface Oral Health Science in Sendai　2007/02/18

  134. Real-Time PCR Analyses of Genera Veillonella and Streptococcus in Healthy Supragingival Plaque Biofilm Microflora of Children. International-presentation

    Junko Matsuyama, Takuichi Sato, Nobuhiro Takahashi, Michiko Sato, Etsuro Hoshino

    The 2nd International Symposium for Interface Oral Health Science in Sendai　2007/02/18

  135. Detection of Periodontopathic Bacteria in Periodontal Pockets by Nested Polymerase Chain Reaction. International-presentation

    Takuichi Sato, Yuki Abiko, Gen Mayanagi, Junko Matsuyama, Nobuhiro Takahashi

    The 2nd International Symposium for Interface Oral Health Science in Sendai　2007/02/18

  136. The effect of amylase and its inhibitors on acid production from starch by Streptococcus mutans and Streptococcus sanguinis International-presentation

    S. Aizawa, H. Miyasawa-Hori, H. Mayanagi, N. Takahashi

    The 2nd International Symposium for Interface Oral Health Science in Sendai　2007/02/18

  137. Inhibitory effects of maltotriitol on the growth and adhesion of mutans streptococci International-presentation

    Harumi Miyasawa-Hori, Shizuko Aizawa, Jumpei Washio, Nobuhiro Takahashi

    The 2nd International Symposium for Interface Oral Health Science in Sendai　2007/02/18

  138. Mutans streptococci and non-mutans streptococci International-presentation

    N. Takahashi

    ORCA Symposium Japan　2006/11/13

  139. フッ化物徐放性修復材料溶出液は齲蝕関連細菌の酸産生を抑制する.

    中條和子, 高橋雄介, 騎馬和歌子, 今里聡, 高橋信博

    第125回歯科保存学会秋季学術大会　2006/11/09

  140. Eluate from fluoride-releasing restorative materials inhibits acid production of caries-related bacteria

    NAKAJO Kazuko, TAKAHASHI Yusuke, KIBA Wakako, IMAZATO Satoshi, TAKAHASHI Nobuhiro

    2006/10/11

  141. 乳幼児プラークの酸産生能、アルカリ産生能と齲蝕増加との関連

    清水弘一, 五十嵐公英, 熊耳隆洋, 高橋信博

    第54回口腔衛生学会・総会　2006/10/08

  142. 唾液細菌および口腔Veillonellaによる口臭成分の一つ硫化水素の産生とその唾液や口腔環境との関わり

    鷲尾純平, 佐藤拓一, 竹内裕尚, 高橋信博

    第55回口腔衛生学会・総会　2006/10/08

  143. 厚生労働省許可特定保健用食品の歯科における課題と展望

    高橋信博

    55回日本口腔衛生学会学術大会　2006/10/06

  144. ミュータンスレンサ球菌のデンプンからの酸産生とそのアミラーゼ阻害剤による影響

    相澤志津子, 宮澤―堀はるみ, 真柳秀昭, 高橋信博

    第48回歯科基礎医学会学術大会　2006/09/22

  145. マルトトリイトールのミュータンスレンサ球菌に対する増殖及び菌体付着抑制効果

    宮澤―堀はるみ, 相澤志津子, 高橋信博

    第48回歯科基礎医学会学術大会　2006/09/22

  146. 唾液ゲラチナーゼ活性とその口腔内での活性変動

    三好慶忠, 渡邉誠, 高橋信博

    第48回歯科基礎医学会学術大会　2006/09/22

  147. 口腔Veillonellaによる硫化水素産生に関する検討―菌種・基質・環境pHによる違い―

    鷲尾純平, 高橋信博

    第48回歯科基礎医学会学術大会　2006/09/22

  148. 歯肉縁下プラーク倍フィルムの多様性解析から見たPorphyromonas gingivalisとStreptococcus gordoniiの関連性

    安彦友希, 佐藤拓一, 真柳弦, 高橋信博

    第48回歯科基礎医学会学術大会　2006/09/22

  149. フッ化物は酸性環境、エタノールはアルカリ環境でう蝕関連菌Enterococcus faecalisとStreptococcus mutansの糖代謝を抑制する

    中條和子, 川嶋順子, 丸尾将太, 山下宗, 高橋信博

    第48回歯科基礎医学会学術大会　2006/09/22

  150. 小児の歯垢細菌叢のStreptococcus、Veillonellaの定量解析

    松山順子, 佐藤拓一, 高橋信博, 佐藤ミチ子, 星野悦郎

    第48回歯科基礎医学会学術大会　2006/09/22

  151. 口腔環境と微生物生態系―多様で微細な小宇宙（ミクロコスモス）

    高橋信博

    第48回歯科基礎医学会学術大会　2006/09/22

  152. 東北大学歯学部の新しい教育カリキュラム ？アドバンス科目の導入？

    笹野泰之, 小関健由, 小松正志, 高田春比古, 高橋信博

    第25回日本歯科医学教育学会・総会　2006/06/16

  153. 歯科医学教育の質の向上を考える

    高橋信博

    第25回日本歯科医学教育学会　2006/06/16

  154. Predominance of Anaerobes in Crevices around Titanium Orthodontic Anchor Plates International-presentation

    R. Sato, T. Sato, I. Takahashi, J. Sugawara, N. Takahashi

    第84回IADR　2006/06/11

  155. Metabolic Properties of Hydrogen Sulfide Production by Oral Veillonella. International-presentation

    J Washio, K. Nakajo, T. Sato, S. Matoba, T. Seki, N. Yamamoto, M. Yamamoto, N. Takahashi

    第84回IADR　2006/06/11

  156. Profiling of Root-canal Microflora Before and After Root-canal Treatments International-presentation

    Y. Ito, T. Sato, G. Mayanagi, K. Yamaki, H. Shimauchi, N. Takahashi

    第84回IADR　2006/06/11

  157. pH-tolerant Acid Production from glucose by Enterococcus faecalis International-presentation

    K. Nakajo, J. Washio, S. Aizawa, H. Miyasawa-Hori, T. Sato, N. Takahashi

    第84回IADR　2006/06/11

  158. α-Amylase and Its Inhibitors Affect Starch Fermentation by Streptococcus mutans International-presentation

    S. Aizawa, H. Miyasawa-Hori, H. Mayanagi, N. Takahashi

    第84回IADR　2006/06/11

  159. Non-collagenous Bone Proteins at Interface of Epithelial Cells and Fibroblasts International-presentation

    M. Shimonishi, J. Hatakeyama, Y. Sasano, N. Takahashi, M. Komatsu

    第84回IADR　2006/06/11

  160. 食品の酸産生評価の現状と問題点

    高橋信博

    第53回日本口腔衛生学会　2004/09/18

  161. キシリトールによる増殖抑制効果のStreptococcus mutans菌株間での違い

    角田 初恵, 田中 光郎, 高橋 信博

    Journal of oral biosciences　2004/09/01

  162. エコロジカルな視点から見たう蝕病因論

    高橋信博

    第42回日本小児歯科学会　2004/05/20

  163. 小児口腔内からのミュータンスレンサ球菌のPCR法による検出について

    松山 順子, 高橋 信博, 野田 忠

    小児歯科学雑誌　2004/04/25

  164. Dental plaque cariogenicity from ecological point of view

    高橋信博

    第51回JADR学術大会　2003/12/01

  165. サンドイッチELISA系を用いた歯垢・唾液中のGTF-B量の定量とS. mutans菌数レベル及びDMFTとの相関について

    佐藤 廉也, 佐藤 拓一, 高橋 一郎, 高橋 信博

    歯科基礎医学会雑誌　2003/09/01

  166. Real-time 定量 PCR を利用した歯垢内細菌の層別密度分布の分析

    加藤 一夫, 福井 敬子, 中垣 晴男, 佐藤 拓一, 高橋 信博

    口腔衛生学会雑誌　2003/08/30

  167. う蝕原性菌を再考する

    高橋信博

    第50回JADR　2002/11/30

  168. 小児歯垢中に存在するS.mutansとS.sobrinusのPCR法による検出頻度の各年齢層別の比較

    松山 順子, 佐藤 拓一, 高橋 信博

    歯科基礎医学会雑誌　2002/09/20

  169. Microbiological comparison between root caries lesion and proximal dental plaque.

    T Wakui, M Nakano, M Yajima, Y Momoi, T Sato, N Takahashi, N Maeda

    JOURNAL OF DENTAL RESEARCH　2002/03

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Research Projects 58

  1. 口腔マイクロバイオームの新・存在意義～全身・口腔健康に寄与する亜硝酸産生の全容～

    鷲尾 純平, 高橋 信博, 安彦 友希, 互野 亮, 佐藤 拓一, 柳田 保子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2024/04/01 - 2028/03/31

  2. 早期小児齲蝕（ECC）関連細菌Scardoviaの増殖を促す口腔環境因子の同定

    安彦 友希, 川嶋 順子, 高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2023/04/01 - 2027/03/31

  3. 口腔細菌叢で脳をまもる：抗認知症プラズマローゲンを産生する嫌気性菌の同定と応用

    高橋 信博, 鷲尾 純平, 仲川 清隆

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2023/06/30 - 2026/03/31

  4. 口腔マイクロバイオームの新機能：「副代謝系」の解明による口腔と全身の健康増進戦略

    高橋 信博, 佐藤 拓一, 鷲尾 純平, 坂本 光央

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2024/04/01 - 2025/03/31

  5. 呼気オミックスを用いた乳がん口腔支援システムの開発

    丹田 奈緒子, 石田 孝宣, 赤池 孝章, 鷲尾 純平, 多田 寛, 高橋 信博, 小関 健由

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

  6. 口腔マイクロバイオームの新機能：「副代謝系」の解明による口腔と全身の健康増進戦略

    高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(B)

    Category: 基盤研究(B)

    Institution: 東北大学

    2021/04/01 - 2025/03/31

  7. 『次代歯周病予防薬』開発のための『選択的予防メカニズム』の解明

    土門 ひと美, 鷲尾 純平, 高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2021/04/01 - 2024/03/31

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    Porphyromonas gingivalis(Pg)、Prevotella intermedia、Fusobacterium nucleatumといった歯周病関連菌は、タンパク質やジペプチドを特異的に代謝し増殖する。現在、歯周病の予防・治療薬は、クロルヘキシジンなどの非特異的殺菌剤が主流である。しかし、これら細菌のタンパク質・ジペプチド代謝を選択的に阻害する薬剤を見い出せれば、歯周病を『選択的に予防』でき『口腔マイクロバイオームの健全化』を図ることが可能となる。 令和３年度は、フッ化物によって阻害される新たな酵素の特定を試みた。令和元年度に、Pg ATCC33277Tの最終代謝産物および菌体内中間代謝物の量の変化を、それぞれHPLCおよびCE-TOFMSを用いて測定した。フッ化物の共存により、細菌数当たりの最終代謝産物量がいくつか増加していると共に、その代謝経路上にある幾つかの中間代謝物の減少がみられた。これらのことから、減少している中間体産物が他の代謝経路に流れていることが想定された。そこで、他の代謝経路に流れていると想定される反応系の中間代謝物を基質とし、超音波粉砕により菌体から抽出した酵素を用いて、フッ化物による酵素活性の影響を検討した。複数の酵素がフッ化物阻害対象として特定されつつあり、現在、阻害効果の最終確定を進めているところである。

  8. 新視点で探る口腔バイオフィルム細菌叢の存在意義～硝酸還元活性と全身・口腔健康～

    鷲尾 純平, 佐藤 拓一, 高橋 信博, 互野 亮

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2020/04/01 - 2023/03/31

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    口腔バイオフィルムは、これまでう蝕、歯周病、誤嚥性肺炎等の様々な病原因子としての“負”の作用がクローズアップされてきた。一方、構成細菌の持する硝酸還元活性により産生される亜硝酸塩は、血液循環の改善や、一部の口腔細菌の増殖を抑制することが近年示され、我々の健康維持に寄与しうるという“正”の作用を持つ可能性に注目が集まっている。 そこで私たちはこれまで、口腔バイオフィルム中の硝酸還元活性を持つ菌について網羅的探索を行い、口腔内における亜硝酸産生を担う細菌種として、Actinomyces, Shaalia, Veillonella, Neisseria, Rothia属がその大半を占めることを明らかにしてきた。 さらに、前述の細菌種のうち、いくつかを用いて、酸素濃度、pH、亜硝酸濃度、糖濃度、乳酸濃度等、種々の環境因子を変動させた際の硝酸還元活性の変化を測定した。その結果、いずれの菌においても、環境条件により、その産生能を大きく変動させることが明らかとなり、その変動は菌種によって傾向が異なることが示唆された。 本年度は、引き続きどのような条件下で最も効率的に亜硝酸が産生されるのかについて検討するとともに、亜硝酸をさらに分解する菌の探索にも着手し、いくつかの菌種で分解活性を持つことを認めた。なお、当初予定していた実際のプラーク試料をもちいた検討は、コロナの感染対策の影響により、少し計画を順延した。 次年度も引き続きこれらの点について明らかにしながら、研究を進める予定である。また、研究内容の公表に向けても取り組みたい。

  9. 脱灰・タンパク質分解包括モデルによる根面う蝕機序解明と予防・治療法の検討

    真柳 弦, 柳田 保子, 鷲尾 純平, 高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2019/04/01 - 2022/03/31

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    う蝕は、歯面に付着するバイオフィルム中の細菌が糖代謝し、産生した酸により歯の表面が脱灰されて生じるため、歯質脱灰を検討する際には、pHやカルシウムイオン（Ca2+）濃度などを同時かつ連続的に測定するマルチイオンモニタリングが必要である。 今年度は、マルチイオンモニタリングによる無機質脱灰評価を可能にするため、カルシウムイオン濃度変化と同時に、ナトリウムイオン、カリウムイオンやpHなどを連続的に測定可能なマルチイオンマイクロデバイスの開発を目指し、複数個の小型な平面形状のイオン選択電極（Solid Contact Ion Selective Electrode(SC-ISE)）を作製した。これを用いてカルシウムイオン濃度応答性を検討した。 イオン選択電極を作製する基板をポリイミドフィルムからポリエチレンテレフタラート(PET)フィルムに変更し、金を蒸着してAu電極を作製することによりイオン選択膜の電極基板への密着性向上を図った。またイオン－電子交換体として導電性ポリマー(PEDOT-PSS)を用い、イオン-電子交換体とイオン選択膜の層を形成した。参照電極の作製は銀/塩化銀(Ag/AgCl)インクを用いた。カルシウム標準溶液(1×10-8～10-1M)を用いて1.0sごとにCWISE、SC-ISEの計2電極の電位を測定した。SC-ISEは62.3mV/log[Ca2+]、CWISEは63.3mV/log[Ca2+]の傾きが得られ、動作範囲はSC-ISE、CWISEともに1.0×10-1～1.0×10-3の間であった。 今年度作製したSC-ISEは薄膜化、構造の簡素化を実現しながら、動作範囲を向上させることができたため、今後のマルチイオン測定デバイスの開発に貢献できると考える。

  10. 包括的糖代謝プロファイリングによるScardoviaの齲蝕関連因子の特定

    安彦 友希, 高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2018/04/01 - 2022/03/31

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    Scardovia wiggsiaeは、最近の研究で重度の早期小児齲蝕（Early Childhood Caries：ECC）や青年期における白斑齲蝕病変から検出されており、新たな齲蝕関連細菌として注目されている。Scardovia属はフルクトース6リン酸経路（F6PPK shunt）という特有の糖代謝経路を有することから、従来の齲蝕関連細菌とは酸産生能や代謝産物、さらにはフッ化物に対する感受性等、糖代謝の性質が異なることが予測される。そこで実際の口腔を模した環境でS. wiggsiaeの糖代謝の性質を明らかにし、その齲蝕関連性について検討した。 S. wiggsiae C1A-55株及びS. mutans NCTC10449株を用い、グルコースを添加した際の酸産生活性測定、代謝産物の同定、またフッ化カリウム（KF）を介在させた場合の酸産生活性の変化について検討した。実験は全て嫌気条件下で行った。 その結果、S. wiggsiaeはS. mutansよりも酸産生活性は低かったものの、pH 5.5の酸性環境においても酸を産生し続けた。またS. mutansよりも高いフッ化物抵抗性を示した。産生された酸の成分は、S. wiggsiaeは酢酸、S. mutansは乳酸がメインであった。S. wiggsiaeが高いフッ化物抵抗性を示した原因として、S. wiggsiaeのF6PPK shuntはフッ化物の影響を受けにくいことが推察された。そこで、メタボローム解析を用いた細胞内代謝中間体の網羅的解析を行なったところ、F6PPK shuntはKFによる代謝阻害を受けずに酸産生能力を維持していることが明らかとなった。さらにS. wiggsiaeの主要な代謝産物である酢酸は、pH の低い酸性環境下では乳酸よりも歯の内部に浸透しやすいことが報告されており、歯を脱灰し、う蝕の進行を早める可能性が高くなると考えられた。

  11. Do Bacteria Cause Cancer? Elucidation of "cell-microbiome interaction" using metabolism as an indicator

    Takahashi Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2018/06/29 - 2021/03/31

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    It is becoming clear that bacteria are involved in the canceration of cells. Therefore, in this study, we examined the effect of bacteria on cells using metabolism as an indicator using "real-time metabolism monitoring system" that we developed. As a result, the following were clarified. It was found that (1) Porphromonas gingivalis (Pg), which is the most representative periodontal disease-associated bacterium among oral bacteria, suppresses cellular glucose metabolism, (2) the cell glucose metabolism inhibitor is present in the culture supernatant of Pg, and (3) it is not a Pg-specific proteolytic enzyme gingipain but a completely different protein.

  12. Development of predictive oral supportive system for perioperative breast cancer patients

    Tanda Naoko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2018/04/01 - 2021/03/31

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    Bone is the most frequent metastatic site of breast cancer, and metastases cause a poor quality of life due to bone pain, pathological fracture, hypercalcemia, and spinal cord compression. Bone metastases in breast cancer patients are a common concern for medical doctors and dentists. Bone-modifying agents, which are necessary to prevent skeletal-related events, are associated with osteonecrosis of the jaw as an adverse side effect. Prognostic risk factors of bone metastases are important for predictive oral supportive system for breast cancer patients. Univariate analysis and multivariate analysis were performed using the Cox proportional hazards model to investigate associations between prognostic variables and time to bone metastasis in primary operable breast cancer patients. In multivariate analysis, tumor stage and alcohol sensitivity were significant prognostic risk factors for bone metastases.

  13. What is a healthy oral microbiome? Proposal of oral health indicators based on metabolic resilience

    Takahashi Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2017/04/01 - 2021/03/31

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    1. The "metabolic database of oral microbiome" was expanded by newly discovering glucose metabolism of new caries-associated bacteria Bifidobacaterium and Scardvia, nitrate metabolism of Veillonella and Neisseria, and ethanol metabolism system of Streptococcus and Neisseria. 2. These new caries-related bacteria mainly produced acetic acid from sugars, and their metabolism showed high resistance to fluoride. 3. Furthermore, nitrate metabolism was biochemically coupled with lactic acid metabolism (lactic acid consumption), and the consumption of lactic acid improves the caries-induced environment, and nitrite, which is a nitrate metabolite, has an antibacterial effect. It has been clarified that multiple metabolic systems in the microbiome are coordinated with each other to bring about metabolic resilience.

  14. How is the nitrite contributing to our general and oral health promotion produced in oral microflora?

    WASHIO JUMPEI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2017/04/01 - 2020/03/31

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    In recent years, it attracts attention that oral bacteria can metabolize nitrate included in yellow-green vegetables or saliva to nitrite, that has antibacterial activity and vasodilatation effect, and which may contribute to maintain our general and oral health.However, the details about the production of nitrite in the oral cavity is still unknown. Therefore, we tried to clarify the whole aspect of nitrite production in the oral cavity. The nitrite-producing activity in the oral cavity was higher in dental plaque than tongue coatings. The interindividual differences was also higher. It was clarified that the bacterial species such as Actinomyces, Shaalia, and Veillonella species known as commensal bacteria mainly contribute to the nitrite production in oral cavity. In addition, it was also clarified that those nitrite-producing activity can be fluctuated by the various environmental factors such as an oxygen concentration, pH and the lactate concentration.

  15. Developing the model using pH/calcium fluorescent imaging for prevention and treatment of dental caries

    MAYANAGI Gen

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2016/04/01 - 2020/03/31

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    This study aimed to develop the methods to assess pH change at the interface between material or tooth and bacteria continuously under the same kind of conditions as an oral microenvironment in vitro. The fluoride releasing materials and the silver diamine fluoride coated tooth inhibited pH fall at the interface between material/tooth and bacteria. The degree of inhibition of pH fall seemed to correspond to the amounts of fluoride and silver detected, suggesting that the fluoride and silver released from these materials inhibited pH fall.

  16. Comprehensive analysis in oral microbiome as a promoting factor for esophageal cancer

    TANDA NAOKO, ITO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    Acetaldehyde is a powerful carcinogen in thoracic esophageal cancer. The aim of this study is to evaluate perioperative change of mouth acetaldehyde and its causal oral bacteria in thoracic esophageal cancer patients under intensive oral care. Analysis was done compared to control cases. Drinking and smoking histories were longer and oral condition was worse in patients group than in control. Acetaldehyde in mouth air of thoracic esophageal cancer patients decreased after oral intensive care before operation. It also decreased after operation. It is suggested that intensive oral care for perioperative thoracic esophageal cancer patients decreases acetaldehyde produced in oral microbiome.

  17. The elucidation of the pathogenic activity of periodontal disease-related bacteria by metabolomics approaches; The establishment of new method to monitor their metabolic activity in real-time

    Washio Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04/01 - 2018/03/31

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    It has been suggested that the periodontal disease-related bacterial "metabolism" was strongly related to the pathogenicity of periodontal disease. However, they were still unclear because the study about their metabolism was not done enough by technical limitation. Therefore, in this KAKENHI project, I established the new method to monitor their metabolic activity easily in real-time and improved the technical method for the metabolome analysis of periodontal disease-related bacteria. The results in this study suggested that their metabolism was affected greatly by the environmental factors and drugs in actual. These new methods and knowledges could contribute to elucidate the onset mechanism of periodontal diseases and the development of new strategy for their treatment and prevention.

  18. Metabolome analysis of oral biofilm: a possible risk assessment of oral diseases using oral microbiome metabolism

    TAKAHASHI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    This study aimed to clarify the whole picture of metabolic systems of oral biofilm through the metabolome analysis and metabolic activity measurement, and to consider an assessment of oral health and disease using oral microbiome metabolism. The CE-MS metabolome system enabled to analyze the metabolites associated with carbohydrates and amino acids comprehensively and revealed the whole picture of metabolic systems of carbohydrates and amino acids in the oral biofilm. In addition, the metabolic activity of a tiny amount of oral biofilm was successfully measured using the alamarblue system. Furthermore, the metabolic activity was diverse among sample sites and metabolic substrates, suggesting that these metabolic characteristics of oral microbiome can be used as an indicator of oral health and disease.

  19. Development of new evaluation system of mucosal inflammation based upon vascular imaging and preventive method of oral mucositis with oral mucosa mapping system

    KOSEKI Takeyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    To prevent the oral mucosal problem and to enhance the promotion of oral-related QOL, a preventive method of oral mucositis and mucosal injure was built for the elderly suffering xerostomia and for the patients who were undergoing perioperative medical treatments. The updated technology of visual examination of mucosa, so-called Narrow Band Imaging, was employed in this system to visualize the abnormality of oral mucosa. The oral mucosa mapping system which covered whole oral cavity was also developed. By using these two systems, new diagnostic method of oral mucosa was proposed to prevent oral mucositis. Finally, the comprehensive guideline of diagnosis and treatment of oral mucositis were discussed.

  20. Metabolomics of cancer tissue -challenge to the paradoxical energy metabolism of cancer cells

    TAKAHASHI Nobuhiro, TAKAHASHI Tetsu, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04/01 - 2015/03/31

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    This study aimed to characterize the metabolic system of oral squamous cell carcinoma (OSCC) by metabolome analysis. The metabolome profiles were obtained from OSCC and its surrounding normal tissues, using CE-TOFMS. Enhancement of glucose consumption and lactate production (Warburg effect) was observed in OSCC tissues, while the downstream intermediates in the glycolysis were decreased and glutamine degradation (glutaminolysis) was enhanced . These results suggest that OSCC tissues supply metabolic intermediates from glucose for biosynthesis via the pentose phosphate cycle and obtain energy from glutamine with lactate production.

  21. Investigation of the role of MMP in bone healing with mineral and proteome analyses

    SASANO YASUYUKI, NAKAMURA Megumi, TAKAHASHI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2011/04/28 - 2015/03/31

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    The present study was designed to investigate the process of calcification during bone healing in a standardized rat calvarial bone defect model. A standard defect with diameter 3.8 mm was made on the parietal bone of 12-week-old male Wistar rats under anesthesia. The healing bone was analyzed with micro-computed tomography and energy dispersive X-ray spectroscopy in weeks 1,2,4 and 8. The bone mineral density and the Ca/P ratio increased, whereas the C/Ca and C/P ratios decreased during bone healing. The present study demonstrated that the mineral components increase in density and mature in quality while organic components decrease. Our following study revealed the comparable calcification process during development of rat calvarial bone development.

  22. Metabolomic approach to oral biofilm and saliva: possible risk indicator for oral diseases

    TAKAHASHI Nobuhiro, KOSEKI Takeyoshi, SENPUKU Hidenobu, SAKAMOATO Mitsuo, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2010/04/01 - 2014/03/31

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    Metabolome analysis for oral biofilm and saliva using CE-TOFMS was established. This method made it possible to analyze not only sugar metabolism but also amino acid metabolism in oral biofilm and even in saliva, where only small amount of metabolites (about 1/100 of oral biofilm) was contained. In addition, the analyses of the effect of sugar alcohols and fluorides on the biofilm metabolism suggested close coordination between sugar and amino acid metabolisms. Metabolome profiles of sugar and amino acid metabolisms can be used for risk indicators of dental caries and periodontitis/oral malodor, respectively.

  23. Formulation of the rare sugar delivery system for functional food and the safety assessment method

    SHIRAI Yoko, TAKAHASHI Nobuhiro, KAMIMURA Wataru, KANETAKA Hiroyasu, SHIMIZU Yoshinaka, MATSUO Tatsuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: National Institute for Materials Science

    2009/04/01 - 2014/03/31

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    Although it has been difficult to supply a large amount of rare sugars, an inexpensive synthetic method on a large scale was recently established, leading to the reports on various physiological functions including regulation of blood glucose level and suppression of body fat accumulation. Meanwhile, it has been pointed out that rare sugars are difficult to utilize as foodstuffs by reason that they are excreted after the gut absorption more rapidly than typical monosaccharide. Therefore in present study, by applying drug delivery systems (DDS), we tried to bring the physiological functions of rare sugars into action efficiently by means of improving the stability in the body and prolonging the residence time. In addition, safety assessment methods for functional foods containing saccharides such as those exerted in the oral cavity were examined.

  24. Parameters of oral environment for perioperative patients of lung cancer based on two dimensional analysis of breath gasses

    TANDA Naoko, HOSHIKAWA Yasushi, TAKAHSI Nobuhiro, KOSEKI Takeyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2011 - 2013

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    We analyzed gasses from exhaled and mouth air and saliva of perioperative patients of lung cancer at bedside before oral care, after oral care before operation, and after operation. Protective reflexes (cough and swallowing reflex) were also measured before and after operation. As for patients without postoperative pneumonia, oral care before operation increased ratio of salivary streptococci, usually known as healthy-associated bacteria, in anaerobic condition. Oral care also decreased total number of bacteria in saliva and decreased malodorous gasses. Acetone concentration increased after operation with rapid increase of CRP. This study suggests possibility that non-invasive measuring volatile sulfide compounds and volatile organic compounds at bedside indicate oral environments and general inflammatory conditions of perioperative lung cancer.

  25. Metabolomic approach to metabolic biology of cancer cells

    TAKAHASHI Nobuhiro, ECHIGO Seishi, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

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    Metabolome analyses of oral squamous cancer (OSC) were performed by CE-TOFMS, targeting metabolites of Embden-Meyerhof-Parnas pathway (EMPP),pentose-phosphate pathway, TCA cycle and metabolic pathways for amino acids. Method for metabolome analyses of OSC was established. The results suggest that the enhancement of EMPP (Warburg effect) occurred in OSC, as previously reported for other cancers, and that amino acids such as glutamine were utilized as an additional energy source for OSC.

  26. プラズマローゲン含有口腔内細菌を駆使した認知症治療および予防システムの構築

    神尾 好是, 高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的萌芽研究

    Category: 挑戦的萌芽研究

    2009 - 2010

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    ヒト脳の神経細胞のアポトーシスの防御リン脂質プラズマローゲン(Plasmalogen;以降PLと略す)が、本研究代表者によりヒト口腔内細菌Selenomonas sputigenaに発見された。本年度は以下の事実を明らかにした。 (1)Selenomonas sputigenaにおけるプラズマローゲンの詳細を分析した。本菌のプラズマローゲン含量を分析した結果、炭素源がラクトースの場合において、A/P比は0.4であった。本菌由来プラズマローゲン構成脂肪アルデヒド組成を分析した結果、C_<17:1>およびC_<18:1>が主体であり、偶数脂肪アルデヒドおよび奇数脂肪アルデヒドが混在している点が特徴的であった。本菌由来プラズマローゲン構成リン脂質は、ホスファチジールエタノールアミンおよびホスファチジールセリンが主体であった。 (2)アルツハイマー型認知症の原因物質としてアミロイドβ(Aβ)タンパク質が重要である。 AQは細胞膜貫通型のアミロイド前駆体タンパク質(APP)が複数のセクレターゼにより分解されて生じるペプチドである。それらのうち、最終的に作用するのが膜酵素複合体であるγ-セクレターゼである。γ-セクレターゼが包埋されている酵母ミクロソーム画分の脂質をボスファチジールエタノールアミンPLと置き換えることによるγ-セクレターゼ活性を阻害する基礎データを得た。

  27. Development of accelerated remineralization method based upon the non-invasive assessment of mineral density of tooth

    KOSEKI Takeyoshi, TAKAHASHI Nobuhiro, SUZUKI Osamu, TAURA Katsuhiko, IKAWA Kyoko, TANDA Naoko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2008 - 2010

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    Ultrasonic measurement system for mineral density of tooth surfaces had been developed. This system actualized significantly sensitive, non-destructive, and quick measurement. Using this system, we analyzed the relationship between mineral density of tooth and oral environment, especially function of saliva. The result revealed that acid resistivity was correlated with ultrasonic density of tooth mineral. This indicated the possibility of developing new preventive program against tooth decay according to the ultrasonic density of tooth mineral, buffering capacity of saliva, and living practice of daily life.

  28. Contribution of impairment of cough and swallowing reflexes and silent aspiration of oropharyngeal bacteria to the mechanism of postoperative pneumonia

    HOSHIKAWA Yasushi, NODA Masafumi, SADO Tetsu, KONDO Takashi, SATO Takuichi, TAKAHASHI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2008 - 2010

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    This study reconfirmed the close relevance of impairment of cough and swallowing reflexes to postoperative pneumonia after lung resection. We showed the bronchial fluid from patients developing postoperative pneumonia contained considerable oral anaerobic bacteria, by using oral biochemical technique. Our intensive perioperative oral care program, including professional assessment of oral status, dental cleaning, and patient education for self-oral care by a dentist, followed by intensive oral care at least 3 times a day by ICU nurses, and encouragement of self-oral care by floor nurses, tended to decrease anaerobic bacterial amounts in the bronchial fluid and prevented the incidence of postoperative pneumonia.

  29. Development of novel microflora-profiling methods of infected root canals and their application to endodontic therapy

    SATO Takuichi, SHIMAUCHI Hidetoshi, TAKAHASHI Nobuhiro, YAMAKI Keiko, MAYANAGI Gen, MATSUYAMA Junko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2008 - 2010

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    Development of novel microflora-profiling methods of infected root canals and their application to endodontic therapy The aim of this study was to profile the microflora in infected root canals, using anaerobic culture and molecular biological techniques for bacterial identification, before and after root canal treatment. Samples from infected root canals before and after treatment were collected, followed by anaerobic culture. After incubation, CFUs were counted and isolated bacteria were identified by 16S rRNA gene sequencing. The findings of this study suggest that the environment in root canals is anaerobic and therefore support the growth of anaerobes, and that adequate treatment changes the root canal environment drastically.

  30. Two dimensional evaluation by volatile gases for oral condition of diabetes mellitus

    TANDA Naoko, KOSEKI Takeyoshi, HINOKIO Yoshinori, TAKAHASHI Nobuhiro, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2008 - 2010

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    We studied acetone in exhaled air, volatile sulfur compounds and acetone in mouth air, to analyze relation between general condition and oral environment of patients with diabetes mellitus, with healthy volunteers as control. Acetone in exhaled and mouth air of type 2 diabetes patients decreased with improvement of plasma glucose during hospitalization. Study of healthy volunteers with good oral condition showed significantly strong correlation between acetone in exhaled air and acetoacetic acid or 3-hydroxybutyric acid in plasma after fasting overnight. There was no significant correlation between acetone and volatile sulfur compounds. This study suggests possibility that non-invasive acetone measuring indicates ketone bodies in plasma, which reflect pathologic condition of diabetes mellitus.

  31. Development of diagnostic system of gingivitis based upon the objective index of peripheral vessel of inflamed tissues

    TAURA Katsuhiko, KOSEKI Takeyoshi, TAKAHASHI Nobuhiro, IKAWA Kyouko, TANDA Naoko, KOSEKI Ichie, SAITO Keiichi, HOSOKAWA Ryouichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2008 - 2010

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    To distinguish the inflamed gingivae and the healthy gingivae objectively and reproductively, we analyzed the bleeding and rubor, which are known as the clinical symptoms of inflamed tissues. From the researches of the estimation of rubor of inflamed gingival tissues, it might be not useful indicators of inflammation. On the other hand, assessing bleeding upon probing by the standardized probing method with force-controlled probe, would be indicated one of the objective diagnose method of gingivitis.

  32. Metabolome analysis of oral biofilm using CE-TOFMS and its application possibility as an oral health indicator

    TAKAHASHI Nobuhiro, HANADA Nobuhiro, KOSEKI Takeyoshi, SENPUKU Hidenobu, SATO Takuichi, SAKAMOTO Mitsuo

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2007 - 2009

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    Metabolome analysis for the representative plaque bacteria and human oral biofilm using a capillary electrophoresis equipped with a time-of-flight mass spectrometer was established. By this method, a new finding was obtained about the pentose-phosphate pathway and the Kreb's tricarboxylic acid cycle, as well as the Embden-Meyerhof-Parnas pathway. In addition, the metabolome analysis was applicable to a very small amount of oral biofilm. These results suggest that the metabolome data can be used as an indicator of oral health status.

  33. 生体成分のファウリングを阻止して清浄性を維持する人工材料表面の開発

    鈴木 治, 高橋 信博, 佐々木 啓一, 島内 英俊

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 萌芽研究

    Category: 萌芽研究

    Institution: 東北大学

    2006 - 2007

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    平成19年度は、光重合型レジン表面硬化材にリン酸エステルを含浸したものをアクリル表面に塗布し、表面電位(ゼータ電位)、接触角、モデルタンパク質の吸着特性を測定した。タンパク質として、1)牛血清アルブミン(酸性)、2)ミオグロビン(中性)、3)チトクロームc(塩基性)を用いた。表面改質材として、無機フィラー含有硬化材(市販のナノコートラボ)にリン酸エステルモノマーを1、3、5%含有したものをアクリル表面(角材)で光硬化させ表面をコートしてこれを吸着媒とした。タンパク質溶液(25mM Tris-HC1,pH7.2)を角材の表面に滴下し、室温・湿潤雰囲気下で一定時間保持した後、経時的にサンプリングしブラッド法によるタンパクアッセイを行い、上澄みのタンパク濃度からアクリル表面に吸着したタンパク量を間接的に定量した。その結果、リン酸エステルの含有量またタンパク質の種類により吸着特性に違いが見られ、特に酸性および塩基性タンパク質で吸着に抑制効果があることが示唆された。ゼータ電位はアクリルでは弱く正にチャージしていたが、リン酸エステルを含有すると負のチャージとなった。また、接触角はリン酸エステルが増えるほど小さくなる傾向があった。このことからリン酸エステル含有による表面改質が口腔内タンパク質群の電荷の違いによる表面相互作用を調節する可能性およびその後の口腔内細菌の付着の調節につながる可能性が示唆された。 平成18年度に作製検討していたリン酸化ポリビニルアルコールはリン酸化率17.7%の合成に成功した。しかし、アクリル樹脂との相溶性が低く複合体は作製できたが、その均一分散性にやや難点があり課題を残した。解決策としてリン酸エステルの高分子化を図りそれをアクリルの重合時に加えることで相溶性を高められる可能性があり、本研究から新しい材料作製の着想につながった。

  34. Comprehensive and quantitative profiling of microflora of human apical periodontitis

    SATO Takuichi, SHIMAUCHI Hidetoshi, TAKAHASHI Nobuhiro, YAMAKI Keiko, MATSUYAMA Junko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2005 - 2007

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    The purpose of the present study was to profile microflora of root canals before and after root canal therapy, using real-time PCR, PCR-cloning, PCR-sequence analyses based on 16S rRNA genes. Informed consent was obtained from each subject and single-rooted teeth with periapical lesions were investigated. Upon access opening, each dentin sample was collected from the root canal. When the periapical lesion healed clinically through chemo-mechanical cleaning and intracanal medication, the root canal dentin sample was obtained again. The quantification of total bacterial DNA was performed by real-time PCR using universal primers based on 16S rRNA genes. PCR products were cloned and partially sequenced. The partial 16S rRNA gene sequences were then compared with those from the GenBank database using the Blast search program through the website of the NCBI (National Center for Biotechnology Information). The concentrations of bacterial DNA after root canal therapy were lower than those upon access opening. The PCR-cloning and PCR-sequence analyses revealed that Fusobacterium were initially predominant, and that Pseudomonas, Bradyrhizobium and Methylobacterium were predominant after root canal therapy. These results indicated that drastic shifts occurred in microflora of root canals by root canal therapy.

  35. 口腔微生物叢の「揺りかご」:舌苔バイオフィルムの生物学

    高橋 信博, 小関 健由, 佐藤 拓一, 鷲尾 純平, 今井 奨

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 萌芽研究

    Category: 萌芽研究

    Institution: 東北大学

    2005 - 2007

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    本年度は、昨年度に引き続き、口臭原因菌の一つと捉えられる口腔Veillonella属の硫化水素産生活性について検討し、(1)環境因子、とくに口腔内に多く存在する乳酸等の有機酸が硫化水素産生能に与える影響、及び、(2)硫化水素産生に関わる代謝酵素の検索を行った。培養したVeillonella atypica、 V. dispar、 V. parvulaの各標準株(ATCC株)の培養菌にシステインを加えて3時間インキュベートし、産生した硫化水素をメチレンブルー法で定量した。さらにこの反応系に乳酸等の各種有機酸を加え、硫化水素産生活性への影響を検討した。また、培養菌を超音波処理して無細胞抽出液を調整し、システインからの硫化水素産生に関わる酵素活性を検索した。Veillonellaのシステインからの硫化水素産生活性は、乳酸が存在すると著しく増加した。Veillonellaの無細胞抽出液は、システインから硫化水素を産生する酵素活性を示したが、無細胞抽出液に乳酸を添加しても活性の増加は認められなかった。以上のことから、Veillonellaは乳酸の存在下、システインから効率よく硫化水素を産生することが明らかになった。乳酸による促進作用は、酵素の活性化作用ではなく、システインの取込み促進であるものと予測された。口臭源の一つである硫化水素は口腔細菌によって産生されるが、その産生活性はpH(昨年度成果)や有機酸(今年度成果)という口腔環境によって影響を受けるものと考えられ、口腔環境のコントロールによって口臭を抑制し得るものと考えられる。

  36. Establishment of the diagnostic criteria of root caries and the method for its remineralization based on the optical and physical properties of root surfaces

    KOSEKI Takeyoshi, TAKAHASHI Nobuhiro, TAURA Katsuhi, IKAWA Kyoko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2005 - 2006

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    There are no standardized diagnostic criteria of root caries, which occur on the exposed root surface of tooth suffered with periodontitis, especially in elderly people. This absence of evaluation method of decalcification level and mass of soften root surfaces hamper the epidemiological and preventive research of root caries developments. In this study, we evaluated optical measurement with laser and ultrasonic measurement for detecting the mineral loss of root surfaces. Ultrasonic sensor system developed in this study was non-destructive, and small enough to handle in oral cavity. The round shape of front edge of sensor prevents to damage the tooth surfaces during ultrasonic measurements. Furthermore, ultrasonic sensor was placed onto the constant contact-force generator. Then repeatable ultrasonic measurement of decalcification by hand manipulation was achieved. This decalcification detection system of root surface showed reproducible results in considerable clinical conditions, when calibrated with the standards specimen of decalicification. On the other hand, the laser determination method of decalcification was not efficient because root caries and calculus could not be distinguished by using laser fluorescence. These results indicated the diagnostic system of decalcification level of root surfaces will be available by using newly developed non-destructive ultrasonic sensor system. By using this system, the researches of root caries development and preventive method will be proceeded and help to maintain high quality of oral health in elderly people.

  37. Quantification of oral bacterial flora and application to the risk test of oral diseases

    MATSUYAMA Junko, SATO Takuichi, TOMIZAWA Mieko, TAKAHASHI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Niigata University

    2005 - 2006

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    The genus Veillonella is small, nonfermentative, anaerobic, gram-negative cocci. Because Veillonella species obtain their energy by fermenting organic acids, e.g., lactate, this metabolism has the potential to remove a potent, dental-caries producing acid. Therefore, the presence of Veillonella in plaque biofilm may reduce the caries-producing potential of plaque biofilm. The aim of this study was to quantify genera Veillonella and Streptococcus in healthy supragingival plaque biofilm microflora of children. After informed consent was obtained, supragingival plaque was obtained from 44 orally-healthy children (1-16 years). Total bacteria and the target genera (Veillonella and Streptococcus) were quantified by real-time PCR using universal and species-specific primers, respectively, and the proportion of each genus was calculated. The proportion of genera Veillonella and Streptococcus were 2.1±4.1% and 19.4±16.7%, respectively. In addition, the proportion of genus Streptococcus was various among individuals, while that of genus Veillonella was comparatively stable in supragingival plaque biofilm, suggesting that genus Veillonella normally inhabits the mouths of both deciduous and permanent dentition.

  38. Profiling of oral bacterial flora using DNA chips and its application to the risk evaluation of oral diseases of children.

    TAKAHASHI Nobuhiro, HATA Shinji, SATO Takuichi, MATSUYAMA Junko, NAKAJO Kazuko, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2004 - 2006

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    Oral biofilms have been reported to be associated with oral infections such as dental caries and periodontitis. And also, oral biofilms are microbial ecosystems which contain various amounts and sorts of microorganisms, and thus controlling these microbial florae are thought to be one of the ways toward prevention of these oral infections. The aim of this study was to establish DNA micro arrays system to detect oral infections-associated bacteria utilizing species-specific DNA probes of 16S ribosomal RNA genes. Twenty dentinal endodontic lesions-associated bacteria including mutans streptococci and twenty-three periodontitis-associated bacteria including Porphyromonas gingivalis, Tannerella forsythia and Mogibacterium timidum were selected in this study, and the specificity and sensitivity of the DNA micro arrays were tested. In addition, we have been planning to compare the DNA micro arrays system with the direct-＆ real-time PCR on their specificities and sensitivities utilizing one-hundred subgingival plaque biofilms for the present.

  39. Analysis of Molecular and Biological Basis of Systemic Effects Provoked by Periodontal Diseases

    SHIMAUCHI Hidetoshi, KANAYA Sousuke, ITAGAKI Yumi, SUGAWARA Shunji, TAKAHASHI Nobuhiro, SHOJI Kanako

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku university

    2004 - 2006

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    The purpose of this study is to explore the unidentified "missing link" to connect the relationship between periodontal and systemic diseases by molecular biological approaches. For this purpose, we focused on the most potent pathogen of chronic periodontitis, Porphyromonas gingivalis and its components, and analyzed the molecular basis of exerting virulence of this bacterium on both periodontal lesions and bodies in vivo and in vitro. Using in vivo animal models, we investigated a series of studies clarifying ; 1)the role of P.gingivalis infection on hyperlipidemia in periodontitis patients ; 2)the effect of P.gingivalis virulence factors on production of adipocytokines from adipose tissues ; 3)the molecular effects of type 2 diabetes on the healing process of periodontium. First, our in vitro study revealed that P.gingivalis LPS and fimbriae preferentially induced a unique dendritic cell subset with a weak immunostimulatory activity via TLR2-mediated signaling, finally contributing to the immune escape of this bacterium in the periodontal lesion. Furthermore, in vivo studies indicated that infection of P.gingivalis is essential for up-regulation of serum triglycerides and its LPS down-regulated the serum level of adiponectin, suggesting P.gingivalis could exert its virulence after invasion into the systemic circulation. The concept of metabolic syndrome (MetS) has been evolving, that is a clustering of simple clinical measures including waist circumferences, blood pressure, triglycerides, high-density lipoproteins and glucose. This clustering appears depend on two major factors: excess body fat and metabolic susceptibility. Taken our results into this concept, invaded P.gingivalis may work as a MetS risk factor in periodontitis patients via down-regulating the serum adiponectin level. The increased risk of MetS possibly started the sequential of "Metaboic Domino" that increasing the susceptibility to diabetes and coronary heart diseases. Diabetes can delay the healing process of periodontally diseased tissue that may increase the chance of P.gigivalis invasion. In conclusion, P.gingivalis may have an adaptation system by dealing with the local immune defense that finally contributes to systemic-periodontal connection.

  40. DNAチップを用いた口腔微生物叢の網羅的定量的プロファイリング

    高橋 信博, 小関 健由, 竹原 直道, 佐藤 拓一

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 萌芽研究

    Category: 萌芽研究

    Institution: 東北大学

    2003 - 2004

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    本年度は、1.前年度設計した口腔細菌特異的プローブを作成し、DNAチップへの搭載の可否(実用性)を検討し、2.DNAチップへの搭載が可能と考えられた24種のプローブを用いて口腔微生物叢解析用DNAチップを試作し、口腔微生物種特異性、使用法などを検討した。 1.DNAチップ搭載用口腔細菌特異的プローブの作成: プローブの特異性についてはGeneBankなどの既存のDNAデータベースから、プローブのDNAチップへの搭載の可否についてはDNAチップ作成手順などの実用性から検討し、その結果、24種の口腔微生物に対する特異的プローブが完成した。さらに、この1年間で新たに入手した口腔微生物16S ribosomal RNA遺伝子配列データに基づき、新たなプローブを設計・作成中である。 2.DNAチップ搭載用の24種の口腔微生物特異的プローブを用いた口腔微生物叢解析用DNAチップの試作: DNAチップ作成共同研究企業にて、24種の口腔微生物特異的プローブを搭載したDNAチップを試作した。各プローブに該当する標準菌株由来DNAを用いて検討したところ、試作したDNAチップは24種の標準菌体由来DNAを特異的に検出することができることがわかった。さらに、DNAチップ上のスポットは肉眼で明瞭に判別でき、市販の可視光用透過型スキャナーで計測・記録することが十分可能であることが分かった。 現在、実際の臨床サンプルを用いて検討中であり、臨床サンプルに含まれる24種の口腔微生物の従来法による検出(通常のnested PCR法での検出)を終え、DNAチップによる検出との照合を進めつつある。

  41. STUDY OF THE PATHOGENESIS AND PHYSICAL NATURE OF DENTAL BIOFILM

    TAKEHARA Tadamichi, ANSAI Toshihiro, TAKAHASHI Nobuhiro, KOSEKI Takeyoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Kyushu Dental College

    2002 - 2004

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    In the process of developing dental caries on tooth surfaces, characteristic patterns of mineral loss of enamel were observed ahead of collapsing the tooth surface and forming the caries cavity Although this process is affected by microenvironments around the tooth surface, it is difficult to analyze these in detail because of its size and complexity These microenvironments are considered to be determined by several factors, including the presence of microbes and saliva throughout the oral cavity. To understand the microbial factors, we analyzed the interrelationship among the microbes covering the tooth surfaces and host factors which affect the ecology of dental biofilm. Furthermore, to understand the environmental factors, we analyzed biological and physical characteristics of saliva, which play an important role in the microenvironment of the tooth surface. The secretion rates and physical properties of saliva are key factors in the nature of oral circumstances. Thus, physiological properties of saliva, such as viscosity and spinnbarkeit, were analyzed as to their relationship with the pathogenesis and physiological nature of oral cavities. Furthermore, we developed a minute device to obtain data on the microenvironment on tooth surfaces, this device being equipped with semiconductor microprobes for pH and temperature measurement. The memory of this device is sufficiently large to log environmental data for several days. It also includes a highly integrated microcontroller enabling continuous observation of the dynamism of mineral loss. Clinical application of this device to elucidate the nature of the pathogenesis of microbial biofilm will be undertaken to prevent mineral loss due to early dental caries lesions.

  42. Microbiological study on oral bacterial floral shifts in children

    MATSUYAMA Junko, TOMIZAWA Mieko, SATO Takuichi, TAKAHASHI Nobuhiro, NODA Tadashi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Niigata University

    2002 - 2004

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    Mutans streptococci have been implicated as cariogenic bacteria in the oral cavity. It has been reported that the diversity of detection frequencies of mutans streptococci from human dental plaque, and these diversity may be due to the differences of subject age and/or target genes tested. In this study, detection of mutans streptococci from human dental plaque were performed with polymerase chain reaction(PCR) using primers base upon 16S ribosomal RNA, glucosyltransferase(gft) and dextranase(dex) genes, and their detection frequencies were compared. Detection frequencies of mutans streptococci by PCR with primers based upon the 16S rRNA genes were higher than those based upon the gtf and dex genes from human dental plaque of children. Utilizing PCR with primers based upon the 16S rRNA genes, S.mutans were detected from all of the samples tested in this study. On the other hand, the detection frequencies of S.sobrinus were lower (10%) than those of S.mutans, and S.sobrinus were likely detected in the subjects of deciduous attained-dentition and mixed-dentition stage. The 16S rRNA gene-based nested PCR method is a rapid and sensitive method for the detection of mutans streptococci, and may also be suitable for carrying out large-scale studies on the cariogenicity of mutans streptococci. Furthermore, this method can potentially detect not only mutans streptococci but also other oral bacterial species, such as periodontopathic bacteria, by substituting specific primers for each oral bacterial species during the second PCR.

  43. APPLICATION OF SUGAR ALCOHOLS COCKTAILS TO HUMAN CARIES PREVENTION

    TAKAHASHI Nobuhiro, HATA Shinji, NODA Tadashi, MAYANAGI Hideaki, SATO Takuichi, MATSUYAMA Junko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: TOHOKU UNIVERSITY

    2002 - 2003

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    Xylitol inhibits the glycolysis and growth of Streptococcus mutans. We studied the inhibitory effect of xylitol on the acid production of S.mutans at several pH levels under the strictly anaerobic conditions found in the deep layer of dental plaque. Xylitol inhibited the rate of acid production from glucose and changed the profile of acidic end products to formate-acetate dominance, with a decrease in the intracellular level of fructose 1,6-bisphosphate and an intracellular accumulation of xylitol 5-phosphate (X5P). These results were notable at pH 5.5-7.0, but were not evident at pH 5.0. Since the activity of phosphoenolpyruvate phosphotransferase for xylitol was greater at higher pH, it is suggested that xylitol could be incorporated more efficiently at higher pH and that the resultant accumulation of X5P could inhibit the glycolysis of S.mutans more effectively. In addtion, xylitol retarded the growth of mutans streptococci in the presence of glucose (G), galactose (Gal), maltose (M), lactose (L) or sucrose (5) as an energy source, though the inhibition of growth on fructose (Fr) was small. Xylitol inhibited acid production by washed cells of S.mutans from G,Gal,M,L or 5 (12-83% inhibition). S.mutans accumulated xylitol 5-phosphate (X5P) intracellularly through activity of the phosphoenolpyruvate-xylitol phosphotransferase system (PEP-xylitol PTS) when they fermented these sugars in the presence of xylitol. However, in the presence of Fr, no inhibition of acid production was observed. In addition, the amounts of X5P during the fermentationof Fr were smaller than those of other sugars in spite of the presence of PEP-xylitol PTS activity. These results suggest that along with the intracellular accumulation of XSP, xylitol decreases the growth and acid production of mutans streptococci in the presence of various dietary sugars except Fr.

  44. MICROBIOLOGICAL STUDY ON DENTAL CARIES PREVENTION USING SUGAR SUBSTITUTES

    MAYANAGI Hideaki, MATSUYAMA Junko, HATA Shinji, TAKAHASHI Nobuhiro, SATO Takuichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: TOHOKU UNIVERSITY

    2001 - 2003

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    Xylitol inhibits the glycolysis and growth of Streptococcus mutans (S. mutans). In this study, we studied the inhibitory effect of xylitol on the acid production of S. mutans at several pH levels under the strictly anaerobic conditions found in the deep layer of dental plaque. Xylitol inhibited the rate of acid production from glucose and changed the profile of acidic end products to formats-acetate dominance, with a decrease in the intracellular level of fructose 1,6-bisphosphate and an intracellular accumulation of xylitol 5-phosphate (X5P). These results were notable at pH 5.5-7.0,but were not evident at pH 5.0. Since the activity of phosphoenolpyruvate phosphotransferase for xylitol was greater at higher pH, it is suggested that xylitol could be incorporated more efficiently at higher pH and that the resultant accumulation of X5P could inhibit the glycolysis of S. mutans more effectively.

  45. STUDY ON METABOLIC SYSTEMS FOR PEPTIDES AND AMINO ACIDS OF PERIODONTAL PATHOGENS, PORPHYROMONAS AND PREVOTELLA.

    TAKAHASHI Nobuhiro, SATO Takuichi, IWAMI Yoshimichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    1999 - 2001

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    1. Peptides and amino acids as sources for the growth and the energy production of Porphyromonas gingivalis (Pg) and Prevotella intermedia(Pi) Periodontal pathogens, P. gingivalis and P. intermedia utilized peptides, especially peptides containing aspartate and/or glutamate, as the sources of their growth and energy production. In addition, P. gingivalis preferentially utilized aspartylaspartate and glutamylglutamate, while P. intermedia utilized aspartate and aspartylaspartate. The observation that both bacteria had no dipeptidase activity on their cell surfaces indicates that they incorporate dipeptides through a dipeptide-specific transport system. These findings suggest the existence of segregation or competition between P. gingivalis and P. intermedia for their nutrients. 2. Metabolic pathways for peptides and amino acids of P. gingivalis and P. intermedia Metabolic system for peptides and amino acids of P. gingivalis and P. intermedia consists of the following steps: (l) peptide uptake (2) hydrolysis of peptide into amino acid (3) deamination of amino acid into 2-oxoacid (4) oxidation of 2-oxoacid into acyl-CoA and (5) production of short-chain fatty acid. 2-oxoacid oxidoreductases, which catalyze the reaction (4), utilized an artificial electron acceptor, methylviologen. This property is similar to that of enzymes found in archea. 3. Molecular biological method for identification of periodontal pathogens From the ecological point of view as described 1, a molecular biological method for bacterial identification, was developed to know the distribution in dental plaque of periodontal pathogens, P. gingivalis and P. intermedia. A new method, PCR-RFLP based on bacterial 16S ribosomal RNA gene, was found to be not only simple and fast but also more reliable than the conventional method such as biological and morphological tests.

  46. エコシステムとしてのデンタルプラーク-共生と競合のメカニズム-

    高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 萌芽的研究

    Category: 萌芽的研究

    Institution: 東北大学

    1999 - 2000

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    歯周病は、複数種の細菌が共存して病原性を発揮する"混合感染症"である。本研究では歯垢細菌叢生態系のモデルとして、歯周病に関連する細菌と歯周病とはあまり関係ないといわれている細菌を混合し、ホスト由来の蛋白質などと共にインキュベートし、細菌間の共生や競合の様相(蛋白質の利用のされかたや病原因子の変化など)を検討し、そのメカニズムを解明することを目的とした。 1.単独で嫌気的に培養したPorphyromonas gingivalis(Pg)とStreptococcus sanguinis(Ss)を混合し、細菌混合懸濁液を調整した。これにホスト由来の蛋白質としてアルブミン、あるいはアルブミンとグルコースを加えて、嫌気条件、pH7でインキュベートした。アルブミン単独添加ではアルブミンが経時的に分解されたのが、アルブミンとグルコースを添加したものではアルブミンの分解はほとんど起こらなかった。アルブミンとグルコースを添加したものではSsによる糖発酵のためpHが低下しており、このpH低下がPgのプロテアーゼを阻害したためと推測された。 2.単独で嫌気的に培養したPgとFusobacterium nucleatum(Fn)を混合し、細菌混合懸濁液を調整した。これにアルブミンとグルタミン酸を加えて、pH7およびpH5で嫌気的にインキュベートした。pH7ではアルブミンが経時的に分解された。一方、pH5ではインキュベートの初期にはほとんど分解されなかったが、時間とともにpHが上昇し、やがてアルブミンの分解が始まった。これはFnのアミノ酸(グルタミン酸)代謝によってpHが上昇し、Pgのプロテアーゼの阻害を解除したためと推測された。 以上のことから、複数の菌を混合することにより、細菌間の相互作用が明らかになってきた。しかし、本研究計画が採択されてから日が浅く(平成11年11月追加採択)、現在、本研究を強力に推進中である。

  47. 黄連・黄柏の抗菌メカニズムの解明

    山田 正, 高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 萌芽的研究

    Category: 萌芽的研究

    Institution: 東北大学

    1999 - 2000

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    「漢方薬」は免疫増強作用などホスト側の体質の改善を主目的とし、結果として疾患を改善させようとするものが多い。その中でも日常的に「健胃薬」などとして用いられている漢方薬は、副作用の心配が少なく、口腔内局所投与によっても影響が少ないと思われる。そこで、本研究計画では、黄連や黄柏がもつ(1)各種歯周病関連菌の増殖に対する影響、(2)その作用様式、(3)有効成分、(4)プロテアーゼなどの菌体外酵素に対する影響などを明らかにし、これらの漢方薬の抗菌メカニズムを解明することを目的とし、以下の結果を得た。 1.黄連・黄柏の水抽出物は歯周病関連菌Porphyromonas gingivalis、Prevotella intermedia、Actinobacillus actinomycetemcomitans、Actinomyces naeslundiiの増殖を抑制した。一方、齲触関連菌であるStreptococcusとLactobacillusの増殖はあまり抑制しなかった。 2.黄連・黄柏水抽出物の有効成分はベルベリンに代表されるアルカロイドであることが分かった。 3.この抗菌効果の作用様式は殺菌作用であることが明らかになった。 2.さらにPorphyromonas gingivalis、Prevotella intermedia、Actinobacillus actinomycetemcomitansの菌体外プロテアーゼを阻害することが分かった。しかし、プロテアーゼ抑制に必要な黄連・黄柏水抽出物の濃度は増殖阻害に必要な濃度よりも高かった。 以上の結果から、黄連・黄柏は歯周病関連菌に対して抗菌作用を示すことが明らかになった。また、プロテアーゼ活性阻害効果があるものの、抗菌効果の本質は殺菌作用であることが推察された。

  48. Inhibitory effects of sugar alcohols on acid production of dental plaque bacteria

    YAMADA Tadashi, IWAMI Yoshimichi, TAKAHASHI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: TOHOKU UNIVERSITY

    1998 - 1999

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    1. The aim of this study was to examine the effect of temporary exposure of the streptococcal cells to air on the intracellular flux of glucose and sorbitol metabolism by measuring acid excretion, fluorescence dependent on cellular level of NADH, glycolytic intermediates and enzyme activities. The exposure of cells to air decreased the acid excretions during glucose and sorbitol metabolism. The analysis of the glycolytic intermediates and the fluorescence suggested that the reduced level of acid excretion in the air-exposed glucose metabolizing cells resulted from the decrease in pyruvate catabolism. In the presence of sorbitol, the decreased acid production resulted from the reduced rates of the reactions catalyzed by sorbitol-phosphoenolpyruvate phosphotransferase and sorbitol 6-phosphate dehydrogenase because of shortage of substrates for these enzymes in addition to the decrease in pyruvate catabolism. 2. The aim of this study was to examine glucose and sorbitol metabolism in Streptococcus mutans cells exposed and not exposed to air at the coexistence of these compounds by measuring acid excretion, levels of fluorescence dependent on cellular NADH and glycolytic intermediates. A part of bacterial cells grown under strictly anaerobic conditions (anaerobic cells) was exposed temporarily to air (aerobic cells). When glucose was added to the anaerobic cells metabolizing sorbitol, the acid excretion was increased. At the same time, the level of NADH was decreased and then reached to the higher plateau level than that during glucose metabolism. The aerobic cells neither metabolized sorbitol nor contained cellular intermediates. At 2 min after glucose was added in the presence of sorbitol, the acid excretion was started slowly and the intermediates appeared. The level of NADH was decreased at first and then increased. These results suggested that the anaerobic S. mutans cells metabolized glucose and sorbitol simultaneously, and that in the presence of sorbitol the aerobic cells could start to metabolize glucose by addition of glucose at 2 min after glucose was added, as the intermediates (PEP potential) for the glucose transport were accumulated.

  49. 歯周病関連菌の病原性と口腔環境ストレスとの相互作用

    高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 東北大学

    1997 - 1998

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    前年度の結果を踏まえ、引き続きPrevotella intermedia(Pi)およびPrevotella nigrescens(Pn)の検討を行った。 1. 環境変動因子としてグルコースに加え、歯周ポケット浸出液成分としてのアルブミンおよび唾液成分としてのムチンの影響を調べた。Piはアルブミンとムチンをともに利用し菌の増殖は増加した。さらに、菌体外プロテアーゼであるキモトリプシン様活性、エラスターゼ様活性、カゼイン分解活性が増加した。一方、グルコースの存在で菌の増殖は増加するもののプロテアーゼ活性は減少した。 2. 増殖基質によってプロテアーゼ活性が変化したことから、酵素活性調節は基質の代謝と密接に関わっていると考えられた。そこでPiおよびPnの主要代謝経路すなわち(1)アミノ酸代謝経路と(2)グルコース代謝経路を決定した。(1)前年度に決定されたアスパラギン酸代謝経路に加え、さらにバリンとロイシンの代謝経路が決定された。バリンとロイシンはともに分枝アミノ酸トランスアミナーゼと分枝オキソ酸酸化還元酵素によって代謝され、それぞれイソブチル酸とイソバレリアン酸になることがわかった。(2)グルコースはエムデン・マイヤホフ解糖系を経て代謝され、コハク酸、酢酸、ギ酸になることがわかった。さらにグルコースはグルコース6リン酸を経てグリコーゲンとなり菌体内に蓄積されることが明らかになった。 以上の結果から、細胞毒性代謝産物であるアンモニア、イソブチル酸、イソバレリアン酸はアミノ酸代謝によって産生されることがわかった。また、グルコース代謝時は解糖系でのエネルギー獲得効率が良いためアミノ酸代謝が抑えられ、その結果、細胞毒性代謝産物産生量が減少すると考えられる。さらに、グリコーゲン蓄積能は口腔環境中でのこの細菌の生存能力を高める一因と思われる。グルコースによるプロテアーゼ活性の減少はいわゆる「カタボライト・リプレッション」の可能性が高いが、さらなる検討が必要である。

  50. Acid shockによる口腔レンサ球菌の傷害とその回避の生化学的機構

    高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 東北大学

    1996 - 1996

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    歯垢細菌は糖代謝によって酸を産生し、歯表面のpHを数分で中性から4程度の酸性に激変させ、歯の脱灰(う蝕)を引き起こす。しかし同時に低pH環境は歯垢細菌自身にも影響を与え、細菌の酸傷害や適応そして菌叢の変化をもたらすと考えられる。(1)Streptococcus mutansとStreptococcus sanguisにAcid shock(pH7→4)を与え、そのまま60分間低pHの状態においたところ、S. sanguisでは糖代謝能、増殖能とも傷害され、糖代謝系酵素のうちGlucokinase、Phosphofructokinase、Glyceraldehyde phosphate dehydrogenase、Enolase活性が選択的に減少した。一方、S. mutansでは糖代謝能、増殖能とも酸傷害を受けず、糖代謝酵素の失活もなかった(歯基礎誌,1996, Oral Microbiol Immunol, in press)。(2)しかし、S. sanguisは穏やかな酸性環境(pH5.5)に僅か30分間曝されただけで耐酸性が増し、S. mutansの耐酸性には及ばないものの、pH4による酸傷害を回避した(J Dent Res, 1996)(3)さらに歯周炎関連菌であるFusobacterium nucleatum、Prevotella intermedia、Porphyromonas gingivalisは、ペプチドなどの窒素源の存在下、環境pHを上昇させることがわかった(J Dent Res, in press)。以上のことから、S. mutansはウ蝕病巣内など低pHが持続しやすい環境にも適応できること、S. sanguisはpHが変動する歯垢環境にうまく適応し、耐酸性を増すことにより酸傷害を回避できること、さらにF. nucleatum、P. intermedia、P. gingivalisは環境pHを上昇させることで酸傷害を回避し、さらに歯周病関連菌の増殖に好都合な中性環境に変えていくことが考えられた。

  51. Acid shock(急激なpH低下)に対する歯垢細菌の応答の生化学的機構

    高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 東北大学

    1995 - 1995

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    歯表面の歯垢細菌は糖代謝によて酸を産生し、歯表面のpHをわずか数分で中性から4程度の酸性に激変させ、歯質の脱灰(=ウ蝕)を引き起こす。低pH環境はウ蝕というHost側への作用だけではなく、歯垢細菌自身にも影響を与え、歯垢細菌の酸性環境への適応や歯垢細菌叢の変化をもたらす。そこで本研究では、代表的な歯垢細菌Streptococcus mutansとStreptococcus sanguisを“急激なpH低下(pH7→4)"に曝し、そのまま60分間低pHの状態におき、その時の細菌の変化を調べた。 S. sanguisでは糖代謝能、増殖能とも傷害され、そのとき、糖代謝系酵素のうちGlucokinase(GK)、Phosphofructokinase(PFK)、Glyceraldehyde phosphate dehydrogenase(GPDH)、Enolaseの活性が50%以下に減少していた。再びこの菌をpH7の培地に戻しインキュベートすると、これらの酵素活性は約2時間かけて徐々に回復し、同時に糖代謝能も回復した。また、糖代謝能が回復するにつれ増殖能も回復した。一方、S. mutansでは糖代謝能、増殖能とも酸傷害を受けず、糖代謝酵素の失活も見られなかった。 以上のことから、“急激なpH低下"に続く60分間の低pH環境によってS. sanguisの糖代謝能および増殖能は傷害され、この時GK、 PFK、 GPDH、 Enolaseという糖代謝系酵素の失活を伴うことが明らかになった。この菌を再び生育に適した環境に戻すと酵素活性が回復したことから、この酸傷害は可逆的であり、酸傷害により失活した酵素を再活性化するシステムの存在が示唆され、S. sanguisはpHが中性と酸性の間を変動する歯垢環境にうまく適応していることが考えられた。一方、S.mutansは酸傷害を回避し、糖代謝能と増殖能を維持したことから、ウ蝕病巣内など常に低pHが持続しやすい環境にも適応できることが考えられた。

  52. 急激なpH低下(acid shock)に対する歯垢細菌の適応

    高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 東北大学

    1994 - 1994

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    歯表面の歯垢細菌は糖代謝によって酸を産生し、pHを低下させ、その結果、歯質の脱灰(=ウ蝕)を引き起こす。低pH環境はウ蝕というHost側への作用だけではなく、歯垢細菌自身にも影響を与え、歯垢細菌の酸性環境への適応やさらには歯垢細菌叢の変化をもたらす。これまでの研究では、連続培養装置を用いた長時間にわたる歯垢細菌の酸性環境への適応を見ており、わずか数分でpHが中性から4程度の酸性に激変し、1時間ほどで再び中性に戻る歯垢環境の影響を見ているとは言い難い。そこで本研究では、代表的な歯垢細菌Streptococcus mutansとStreptococcus sanguisを"急激なpH低下(pH7→4)"に曝し、その時の細菌の反応を調べた。 1.生存率:pH低下後60分間は、S.mutans、S.sanguis共、ほぼ100%生存した。しかし、120分後には、S.sanguisの約50%が死滅した。2.増殖速度:S.mutansではpH低下後60分でもほとんど増殖速度の変化はなかったが、S.sanguisではpH低下後30分で約1.5時間の、60分で約2.5時間のlag phase(増殖停止期間)が見られた。3.糖代謝能:S.mutansはpH低下後60分でもほとんど糖代謝能の変化はなかったが、S.sanguisでは30分で約70%、60分では約90%が失われた。 以上のことから、"急激なpH低下(pH7→4)"後、短時間(30-60分)で、S.sanguisは死滅しないまでも、その増殖能は低下し、これは糖代謝能(=エネルギー産生能)の傷害によるものと考えられた。一方、S.mutansは"急激なpH低下"による傷害を巧く回避し、増殖能を維持していると考えられ、これが、ウ蝕誘発能の高い歯垢(=酸産性能の高い歯垢)にS.mutansが多くなる因子の一つと思われる。現在、これら、S.mutansとS.sanguisの違いをもたらす原因について検討中である。

  53. 口腔疾患関連菌口腔アクチノミセスの代謝活性-INVITROでの基礎的研究

    高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 東北大学

    1993 - 1993

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    主要な歯垢細菌の一つである口腔アクチノミセスは、動物実験や病巣部細菌層の解析、さらに、付着・凝集能や細胞性免疫惹起能についての研究からウ蝕と歯周疾患双方との関連が考えられてきた。しかし、このような生物学的性質の本質である代謝活性についてはまだ不明な点が多い。そこで、アクチノミセスの基本的エネルギー獲得系である糖代謝系の酵素を調べた。 口腔アクチノミセス(Actinomyces viscosusおよびA.naeslundii)はグリコースからピルビン酸に至る全てのエンデンメイヤホフ解糖系酵素活性を示した。しかし、この糖代謝系に関わるいくつかのリン酸化酵素は他の細菌には見られないユニークな性質を示した。1.解糖初発段階を触媒するグルコキナーゼはGTPとポリリン酸だけを、2.次のリン酸化のステップを触媒するホスホフルクトキナーゼはピロリン酸だけをリン酸供与体として利用し、ATPは両酵素のリン酸供与体とはならなかった。3.重炭酸イオンを固定し、アクチノミセスの特徴的代謝産物であるコハク酸産生に関わるホスホエノールピルビン酸カルボキシキナーゼは、GDPとIDPをリン酸受容体とし、ADPを利用しなかった。このGDP/IDP依存性は細菌由来の酵素にはほとんど見られず、動物細胞に一般的にみられる性質であった。4.また、菌体からはGTP、GDP、ピロリン酸、ポリリン酸が検出され、ATP、ADP以外のリン酸供与体および受容体も実際の菌体内で機能していると考えられた。以上のことから、アクチノミセスはユニークな糖代謝系酵素を持ち、他の多くの細菌とはことなり、ATPやGTPに加え、ピロリン酸やポリリン酸という無機リン酸もリン酸供与体として利用していることが考えられる。このことはリン酸化酵素の進化やアクチノミセスの系統樹上での位置付けを考える上で興味深い。

  54. 歯周病関連菌の代謝活性と歯周病巣環境との相互関係-IN VITROでの基礎的研究

    高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 東北大学

    1992 - 1992

  55. Effects of environmental fluctuations on sugar metabolisms of plaque bacteria.

    YAMADA Tadashi, TAKAHASHI Nobuhiro, IWAMI Yoshimiti, ABE Kazuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (B)

    Category: Grant-in-Aid for General Scientific Research (B)

    Institution: TOHOKU UNIVERSITY

    1991 - 1992

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    When oral streptococci was cultured at low pH in continous culture under strictly anaerobic conditions, more lactate was produced with a concomitant decrease in formate and acetate, compared with at neutral pH. Two mechanisms seemed to be mainly involved in the increased production of lactate at acidic pH in the chemostat. Firstly, lactate dehydrogenase (LDH) had lower optimum pH than that of pyruvate formate-lyase (PFL). Secondly, the amount of LDH was markedly increased due to the acidification in the cells. In contrast to other streptococci S. mutans could produce more acid even at pH 5.1-5.5 because of acid tolerance of ATPase which excrete proton from the cells. These properties were considered to render S. mutans higher cariogenicity. It is essential for plaque microorganisms to keep intracellular NADH/NAD balance under anaerobic conditions. Oral streptococci employed the PFL system to metabolize sorbitol anaerobically. Lactobacilli could regenerate NAD to keep the balance via the conversion of acetate into ethanol and metabolize sorbitol in the presence of availabe acetate, even when PFL was irreversibly inactivated. Actinomyces could regenerate NAD via succinate pathway when bicarbonate was available, and increased the rate of acid production three- to four-fold. These effects were not observed in streptococci. Various environmental factors affect sugar metabolisms of several plaque microorganisms, but these effects are quite different depend on bacterial species.

  56. Experimental device designed for assay and purification of oxygen-sensitive enzymes and its application.

    YAMADA Tadashi, TAKAHASHI Nobuhiro, IWAMI Yoshimiti, ABBE Kazuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Developmental Scientific Research (B)

    Category: Grant-in-Aid for Developmental Scientific Research (B)

    Institution: TOHOKU UNIVERSITY

    1990 - 1992

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    An anaerobic glove box, equipped with a purification apparatus and a spectorophotometer, was manufactured as a trial and employed. Several interesting finding were turned out as follows. Pyruvate formate-lyase (PFL) plays an important role in anaerobic sugar metabolism in oral streptococci. PFL was extremely sensitive to oxygen, and assumed to contain a protein radical near a catalytic site. The reaction of molecular oxygen with the radical seemed to generate further harmful active oxygen and bring about irreversible inactivation of the enzyme rapidly. Reversible inactive form of the enzyme was oxygen torelant since it did not contain the radical, and could be activated by PFL-activating enzyme. Partially purified PFL activating enzyme was quite unstable, but incubation of the enzyme with ferrous ion, selenium ion, pterin, restored a part of the activity. The enzyme seemed to contain "a pterin like cofactor" which was assumed to be exist in xanthine oxidase. The activating enzyme of Streptococcus mutans was triggered by pyruvate, but not oxamate, and quite unstable. The properties of the streptococcal enzyme is considered different from that of Escherichia coli. It is essential for plaque microorganisms to keep intracellular NADH/NAD balance under anaerobic conditions. Oral streptococci employed "the PFL system" to keep the balance. Lactobacilli metabolized acetate into ethanol to oxidize NADH to keep it. Actinomyces could regenerate NAD via succinate pathway when bicarbonate was available, and increased the rate of acid production.

  57. 歯周疾患関連細菌の糖・アミノ酸・タンパク質代謝活性とその調節酵素に関する研究

    高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 東北大学

    1991 - 1991

  58. 口腔レンサ球菌の嫌気的糖代謝の酸素傷害、及び、その回復機構についての研究

    高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 東北大学

    1986 - 1987

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  1. [日本国内] 歯学・歯科医療に関する講演・セミナー

    2001/04/01 - 2008/03/31

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    一般市民に対する歯学・歯科医療に関する講演・セミナー

  2. [日本国内] みやぎ県民大学

    2005/04/01 - 2006/03/31

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    宮城県民を対象とした歯学・歯科医療に関する講義

  3. [日本国内] 一日大学

    2004/04/01 - 2006/03/31

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    仙台第二高等学校にて歯学・歯科医学に関する講義

Media Coverage 1

  1. [日本国内] 読売新聞・日本歯科医師会主催 デンタルヘルスシンポジウム

    2006/06/09

    Type: Newspaper, magazine

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  7. pHテレメトリーを用いた歯科用トクホ食品の検定

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