Details of the Researcher

PHOTO

Shohei Ochi
Section
Graduate School of Medicine
Job title
Assistant Professor
Degree

  • 博士（医学）（京都大学）

  • 修士（医科学）（京都大学）

e-Rad No.
30884217

Research History 3

  • 2020/10 - Present
    東北大学 大学院医学系研究科

  • 2020/04 - 2020/09
    東北大学 大学院医学系研究科

  • 2017/04 - 2019/03
    日本学術振興会 特別研究員 DC2

Education 3

  • Kyoto University　Graduate School of Medicine

    2015/04 - 2020/03

  • Kyoto University　Graduate School of Medicine

    2013/04 - 2015/03

  • Tokyo University of Science　Faculty of Pharmaceutical Sciences　Department of Medicinal and Life Science

    2009/04 - 2013/03

Professional Memberships 5

  • Society for Neuroscience

  • 日本神経精神薬理学会

  • 日本解剖学会

  • 日本神経科学学会

  • 日本分子生物学会

Research Areas 7

  • Informatics / Biological, health, and medical informatics /

  • Informatics / Computational science /

  • Life sciences / Psychiatry /

  • Life sciences / Laboratory animal science /

  • Life sciences / Anatomy /

  • Life sciences / Neuroscience - general /

  • Life sciences / Developmental biology /

Awards 3

  1. Excellence Award for the Training Session for Early-Career Scientists

    2024/07　NEURO2024

  2. JSNP Excellent Presentation Award for CINP 2024

    2024/05

  3. 日本薬学会 第133年会 優秀発表賞

    2013/03　日本薬学会

Papers 9

  1. IntelliProfiler: a novel software pipeline for analyzing multiple animals with a high-resolution home-cage RFID system

    Shohei Ochi, Hitoshi Inada, Noriko Osumi

    bioRxiv　2025/04/16

    Publisher: Cold Spring Harbor Laboratory

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    Abstract There is an increasing need for unbiased behavioral analysis systems that accommodate multi-animal interactions in semi-natural environments. Here we present “IntelliProfiler,” a software pipeline designed to analyze locomotor activity and social proximity using a commercially available, previously validated radio frequency identification (RFID) floor array. IntelliProfiler can track and analyze up to 16 mice simultaneously, enabling long-term assessment of both locomotor and social behaviors over extended periods. Using this system, we found that male mice tend to maintain greater social distances than females, and that group size critically influences male social network dynamics. Furthermore, aging significantly affected both locomotor activity and social interaction in a group size-dependent manner. Additionally, a mouse model of autism spectrum disorder (ASD) derived from aged fathers exhibited hyperactivity and increased social distance compared to controls, indicating altered patterns of social interaction, an ASD-related phenotype. These findings demonstrate that IntelliProfiler facilitates the identification of previously unknown signatures and group dynamics, contributing valuable insights to the field of behavioral neuroscience.

  2. AUTOMATED ANALYSIS OF RODENT BEHAVIOR USING INTELLIPROFILLER: A POTENTIAL TOOL FOR STUDYING NEURODEVELOPMENTAL DISORDER-LIKE MODEL MICE

    Shohei Ochi, Hitoshi Inada, Noriko Osumi

    International Journal of Neuropsychopharmacology　28　(Supplement_1)　i58-i59　2025/02/12

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/ijnp/pyae059.101 　

    ISSN： 1461-1457

    eISSN： 1469-5111

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    Abstract   Autism spectrum disorder (ASD) is a prevalent neurodevelopmental disorder (NDD) characterized by deficits in social communication and repetitive behaviors. With an increased number of children diagnosed with ASD, the need to understand the mechanisms and symptoms of NDDs is growing to develop effective treatments and interventions. Animal models have been instrumental in studying NDDs, employing classical behavioral paradigms such as the open field test and the social interaction test. However, obtaining consistent results is still challenging due to the handling effects of experimenters. To address this issue, we have developed a novel system, IntelliProfiler, enabling automated analysis of rodent behavior. IntelliProfiller allows us to analyze the relative distances between individual mice and the travel distances of multiple mice, each embedded with a transponder, without image analysis. The system provides a unique opportunity to evaluate the relative social distance between individuals and groups, which could indicate affinity to others or anxiety in a social environment. Our preliminary analysis using IntelliProfiler revealed pronounced differences in social interaction between wild-type (WT) littermates and a mouse model of ASD (Pax6 heterozygous mutant; Pax6Sey/+). Notably, our previous study did not observe these differences in the three-chamber test (Yoshizaki et al., 2016). Additionally, Pax6Sey/+ female mice exhibited hyperactive behavior and sleep disturbance, suggesting potential comorbidity with attention deficit hyperactivity disorder (ADHD). Our previous research using Pax6rSey2/+ female rats also supports these findings, which exhibited reduced vocal communication and decreased brain volume (Umeda et al., 2010; Hiraoka et al., 2016). Our data suggest that Pax6Sey/+ female mice may serve as a valuable model for studying severe sex-biased ASD-like features, potentially with concomitant ADHD. The application of IntelliProfiller in studying neuropsychiatric behaviors in NDD model mice holds significant promise, offering several advantages over classical behavior analyses. These advantages include 1) the ability to conduct long-term studies of basal activity in a non-contact setting and 2) quantitative analyses of anxiety in social environments using multiple animals. References 1. Ochi S, Manabe S, Kikkawa T, Osumi N. Thirty Years' History since the Discovery of Pax6: From Central Nervous System Development to Neurodevelopmental Disorders. Int J Med Sci 30; 23(11):6115 (2022). 2. Kikkawa T, Casingal CR, Chun SH, Shinohara H, Hiraoka K, Osumi N. The role of Pax6 in brain development and its impact on pathogenesis of autism spectrum disorder. Brain Res 15;1705:95-103 (2019). 3. Hiraoka K, Sumiyoshi A, Nonaka H, Kikkawa T, Kawashima R, Osumi N. Regional Volume Decreases in the Brain of Pax6 Heterozygous Mutant Rats: MRI Deformation-Based Morphometry. PLoS One 29;11(6):e0158153 (2016). 4. Yoshizaki K, Furuse T, Kimura R, Tucci V, Kaneda H, Wakana S, Osumi N. Paternal Aging Affects Behavior in Pax6 Mutant Mice: A Gene/Environment Interaction in Understanding Neurodevelopmental Disorders. PLoS One 17;11(11):e0166665 (2016). 5. Umeda T, Takashima N, Nakagawa R, Maekawa M, Ikegami S, Yoshikawa T, Kobayashi K, Okanoya K, Inokuchi K, Osumi N. Evaluation of Pax6 mutant rat as a model for autism. PLoS One 21;5(12):e15500 (2010). 6. Osumi N, Shinohara H, Numayama-Tsuruta K, Maekawa M. Concise review: Pax6 transcription factor contributes to both embryonic and adult neurogenesis as a multifunctional regulator. Stem Cells. 26(7):1663-72 (2008).

  3. Multiple Mouse Tracking by Joint Estimation of Matching and Missing Values by using RFID based- and Visual-Tracking

    Naoya CHIBA, Shohei OCHI, Matus TANONWONG, Noriko OSUMI, Koichi HASHIMOTO

    SICE SI SI2024　2024/12

  4. IntelliProfiler: a novel analytic tool for behavior dynamics of multiple animals in a home cage

    Shohei Ochi, Hitoshi Inada, Noriko Osumi

    bioRxiv　2024/10/26

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1101/2024.10.23.619967 　

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    There is a growing demand for sophisticated behavioral analysis systems that minimize bias in multi-animal and semi-natural environments. We present "IntelliProfiler," an advanced system using radio frequency identification (RFID) technology to enable fully automated behavior analyses of multiple mice within a home cage. IntelliProfiler continuously monitors up to 16 mice, capturing both locomotor activity and social dynamics over extended periods. Our findings revealed that male mice maintain broader social distances than females, with group size playing a key role in shaping male social network dynamics. Furthermore, aging in males significantly impacts both locomotor activity and social interaction in a group size-dependent manner. With its ability to provide in-depth analysis of individual and group behaviors, IntelliProfiler offers a novel approach for exploring complex social interactions and group dynamics, advancing the field of behavioral science.

  5. A Transcriptomic Dataset of Embryonic Murine Telencephalon Peer-reviewed

    Shohei Ochi, Shyu Manabe, Takako Kikkawa, Sara Ebrahimiazar, Ryuichi Kimura, Kaichi Yoshizaki, Noriko Osumi

    Scientific Data　11　(1)　2024/06/05

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41597-024-03421-x 　

    eISSN： 2052-4463

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    Abstract Sex bias is known in the prevalence/pathology of neurodevelopmental disorders. Sex-dependent differences of the certain brain areas are known to emerge perinatally through the exposure to sex hormones, while gene expression patterns in the rodent embryonic brain does not seem to be completely the same between male and female. To investigate potential sex differences in gene expression and cortical organization during the embryonic period in mice, we conducted a comprehensive analysis of gene expression for the telencephalon at embryonic day (E) 11.5 (a peak of neural stem cell expansion) and E14.5 (a peak of neurogenesis) using bulk RNA-seq data. As a result, our data showed the existence of notable sex differences in gene expression patterns not obviously at E11.5, but clearly at E14.5 when neurogenesis has become its peak. These data can be useful for exploring potential contribution of genes exhibiting sex differences to the divergence in brain development. Additionally, our data underscore the significance of studying the embryonic period to gain a deeper understanding of sex differences in brain development.

  6. Thirty Years' History since the Discovery of Pax6: From Central Nervous System Development to Neurodevelopmental Disorders. International-journal Peer-reviewed

    Shohei Ochi, Shyu Manabe, Takako Kikkawa, Noriko Osumi

    International journal of molecular sciences　23　(11)　2022/05/30

    DOI： 10.3390/ijms23116115 　

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    Pax6 is a sequence-specific DNA binding transcription factor that positively and negatively regulates transcription and is expressed in multiple cell types in the developing and adult central nervous system (CNS). As indicated by the morphological and functional abnormalities in spontaneous Pax6 mutant rodents, Pax6 plays pivotal roles in various biological processes in the CNS. At the initial stage of CNS development, Pax6 is responsible for brain patterning along the anteroposterior and dorsoventral axes of the telencephalon. Regarding the anteroposterior axis, Pax6 is expressed inversely to Emx2 and Coup-TF1, and Pax6 mutant mice exhibit a rostral shift, resulting in an alteration of the size of certain cortical areas. Pax6 and its downstream genes play important roles in balancing the proliferation and differentiation of neural stem cells. The Pax6 gene was originally identified in mice and humans 30 years ago via genetic analyses of the eye phenotypes. The human PAX6 gene was discovered in patients who suffer from WAGR syndrome (i.e., Wilms tumor, aniridia, genital ridge defects, mental retardation). Mutations of the human PAX6 gene have also been reported to be associated with autism spectrum disorder (ASD) and intellectual disability. Rodents that lack the Pax6 gene exhibit diverse neural phenotypes, which might lead to a better understanding of human pathology and neurodevelopmental disorders. This review describes the expression and function of Pax6 during brain development, and their implications for neuropathology.

  7. Oscillatory expression of Hes1 regulates cell proliferation and neuronal differentiation in the embryonic brain. International-journal Peer-reviewed

    Shohei Ochi, Yui Imaizumi, Hiromi Shimojo, Hitoshi Miyachi, Ryoichiro Kageyama

    Development (Cambridge, England)　147　(4)　2020/02/26

    DOI： 10.1242/dev.182204 　

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    The expression of the transcriptional repressor Hes1 oscillates in many cell types, including neural progenitor cells (NPCs), but the significance of Hes1 oscillations in development is not fully understood. To examine the effect of altered oscillatory dynamics of Hes1, we generated two types of Hes1 knock-in mice, a shortened (type-1) and an elongated (type-2) Hes1 gene, and examined their phenotypes focusing on neural development. Although both mutations affected Hes1 oscillations, the type-1 mutation dampened Hes1 oscillations more severely, resulting in much lower amplitudes. The average levels of Hes1 expression in type-1 mutant NPCs were also lower than in wild-type NPCs but similar to or slightly higher than those in Hes1 heterozygous mutant mice, which exhibit no apparent defects. Whereas type-2 mutant mice were apparently normal, type-1 mutant mice displayed smaller brains than wild-type mice and upregulated proneural gene expression. Furthermore, proliferation of NPCs decreased and cell death increased in type-1 mutant embryos. When Hes3 and Hes5 were additionally deleted, neuronal differentiation was also accelerated, leading to microcephaly. Thus, robust Hes1 oscillations are required for maintenance and proliferation of NPCs and the normal timing of neurogenesis, thereby regulating brain morphogenesis.

  8. The significance of gene expression dynamics in neural stem cell regulation. Peer-reviewed

    Ryoichiro Kageyama, Shohei Ochi, Risa Sueda, Hiromi Shimojo

    Proceedings of the Japan Academy. Series B, Physical and biological sciences　96　(8)　351-363　2020

    DOI： 10.2183/pjab.96.026 　

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    Neural stem cells (NSCs) actively proliferate and generate neurons and glial cells (active state) in the embryonic brain, whereas they are mostly dormant (quiescent state) in the adult brain. The expression dynamics of Hes1 are different between active and quiescent NSCs. In active NSCs, Hes1 expression oscillates and periodically represses the expression of proneural genes such as Ascl1, thereby driving their oscillations. By contrast, in quiescent NSCs, Hes1 oscillations maintain expression at higher levels even at trough phases (thus continuous), thereby continuously suppressing proneural gene expression. High levels of Hes1 expression and the resultant suppression of Ascl1 promote the quiescent state of NSCs, whereas oscillatory Hes1 expression and the resultant oscillatory Ascl1 expression regulate their active state. Furthermore, in other developmental contexts, high, continuous Hes1 expression induces astrocyte differentiation or the formation of boundaries, which function as signaling centers. Thus, the expression dynamics of Hes1 are a key regulatory mechanism generating and maintaining various cell types in the nervous system.

  9. Effects of mouse utricle stromal tissues on hair cell induction from induced pluripotent stem cells. International-journal Peer-reviewed

    Akiko Taura, Hiroe Ohnishi, Shohei Ochi, Fumi Ebisu, Takayuki Nakagawa, Juichi Ito

    BMC neuroscience　15　121-121　2014/11/06

    DOI： 10.1186/s12868-014-0121-7 　

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    BACKGROUND: Hair cells are important for maintaining our sense of hearing and balance. However, they are difficult to regenerate in mammals once they are lost. Clarification of the molecular mechanisms underlying inner ear disorders is also impeded by the anatomical limitation of experimental access to the human inner ear. Therefore, the generation of hair cells, possibly from induced pluripotent stem (iPS) cells, is important for regenerative therapy and studies of inner ear diseases. RESULTS: We generated hair cells from mouse iPS cells using an established stepwise induction protocol. First, iPS cells were differentiated into the ectodermal lineage by floating culture. Next, they were treated with basic fibroblast growth factor to induce otic progenitor cells. Finally, the cells were co-cultured with three kinds of mouse utricle tissues: stromal tissue, stromal tissue + sensory epithelium, and the extracellular matrix of stromal tissue. Hair cell-like cells were successfully generated from iPS cells using mouse utricle stromal tissues. However, no hair cell-like cells with hair bundle-like structures were formed using other tissues. CONCLUSIONS: Hair cell-like cells were induced from mouse iPS cells using mouse utricle stromal tissues. Certain soluble factors from mouse utricle stromal cells might be important for induction of hair cells from iPS cells.

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Misc. 8

  1. 大脳皮質形成過程におけるPax6 下流のδカテニンの発現に関する性差研究への取り組み

    越智 翔平, 眞鍋 柊, Seung Hee Chun, 吉川 貴子, 大隅 典子

    第128回日本解剖学会総会・全国学術集会 プログラム集　[2P-027]　2023/03

  2. IntelliProfiler system: Development of a novel social behavior evaluation system and its application to autism spectrum disorder model mouse

    Shohei Ochi, Hitoshi Inada, Noriko Osumi

    The 21st Takeda Science Foundation Symposium on Bioscience, Towards Understanding Human Development and Evolution, Program & Abstracts　[Poster 059]　2023/01

  3. マウス大脳皮質原基における雌雄差形成に関わる分子メカニズム解明への取り組み

    越智 翔平

    文部科学省科学研究費助成事業 学術変革領域研究 「学術研究支援基盤形成」『先進ゲノム解析研究推進プラットフォーム（先進ゲノム支援）』 ー拡大班会議ー プログラム&要旨集　[45]　2023/01

  4. インテリボード：新規社会行動評価系の開発および自閉症モデルマウスへの展開

    越智 翔平, 稲田 仁, 大隅 典子

    NEURO2022 プログラム集　[1LBA-084]　2022/06

  5. Understanding sex differences in the expression pattern of the δ- catenin, a downstream to Pax6, in the mammalian corticogenesis

    Shohei Ochi, Shyu Manabe, Seung Hee Chun, Kikkawa Takako, Noriko Osumi

    [2P-01-01]　2022/03

  6. 胎生期大脳皮質におけるPax6下流因子δカテニンの発現推移と雌雄差解析

    越智 翔平, 眞鍋 柊, Seung Hee Chun, 吉川 貴子, 大隅 典子

    第44回日本分子生物学会年会プログラム集　[3P-0436]　2021/12

  7. 胎生期マウス大脳皮質における脳構築の遺伝的プログラムの性差研究

    新学術領域研究「個性」創発脳 第 5 回領域会議プログラム集　[P15]　2020/12

  8. Hes1遺伝子の発現振動は胎生期の脳において細胞増殖や神経分化を制御する

    越智 翔平, 今泉 結, 下條 博美, 宮地 均, 影山 龍一郎

    第43回 日本神経科学大会プログラム集　[LBA-001]　2020/07

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Presentations 61

  1. Investigating the Role of G-CSF in a Maternal Immune Activation Mouse Model

    Hinako Kirikae, Sakai Mai, Zhiqian Yu, Hiroaki Tomita, Yoshiyuki Kasahara, Shohei Ochi, Noriko Osumi, Motoko Maekawa, Yuji Owada

    第48回日本神経科学大会　2025/07/26

  2. Analyzing multi-channel LFP during social interactions between two mice using machine-learning

    Kyosuke Osaka, Xiaoying Tuo, Ren Saito, Shohei Ochi, Takako Kikkawa, Ken-Ichiro Tsutsui, Noriko Osumi, Atushi Tamura, Takuya Sasaki

    第48回日本神経科学大会　2025/07/24

  3. Sex-specific behavioral effects of testosterone in group- housed mice

    Masashi Azuma, Shohei Ochi, Jasper Germeraad, Iori Hara, Hitoshi Inada, Noriko Osumi

    第48回日本神経科学大会　2025/07/24

  4. An RFID-vision hybrid tracking system for behavior analysis in group-housed mice

    Rafael Mugi Suzuki, Shohei Ochi, Naoya Chiba, Matus Tanonwong, Hitoshi Inada, Koichi Hashimoto, Noriko Osumi

    第48回日本神経科学大会　2025/07/24

  5. Sex differences in social behavior among autism spectrum disorder model mice: insights from group behavior analyses

    Iori Hara, Shohei Ochi, Masashi Azuma, Hitoshi Inada, Noriko Osumi

    第48回日本神経科学大会　2025/07/24

  6. Study towards understanding the molecular mechanism underlying female-specific ASD pathogenesis in Pax6 mutant mouse, a neurodevelopmental disorder model

    Shyu Manabe, Shohei Ochi, Takako Kikkawa, Mai Saeki, Kohdai Yamada, Hidetaka Kosako, Yusuke Kishi, Tatsuya Sawasaki, Noriko Osumi

    第48回日本神経科学大会　2025/07/26

  7. Analysis of behavioral dynamics of neurodevelopmental disorder model mice in a large-scale group environment

    Shohei Ochi, Hitoshi Inada, Noriko Osumi

    第48回日本神経科学大会　2025/07/26

  8. 集団行動の長期追跡を用いたマウスにおける階層的インタラクションの理解

    越智翔平

    科学研究費助成事業 学術変革領域研究（A）「階層的生物ナビ学」 2025年度 領域会議　2025/07/14

  9. RFID技術を用いたマウス・ハムスターの集団行動パターン比較と疾患モデル解析

    越智翔平, 梶原将大, 直亨則, 高林健人, 稲田仁, 大隅典子

    科学研究費助成事業 学術変革領域研究（A）「階層的生物ナビ学」 2025年度 領域会議　2025/07/13

  10. 大集団飼育環境における神経発達障害様行動特徴の解析

    越智翔平, 稲田仁, 大隅典子

    第34回神経行動薬理若手研究者の集い　2025/03/16

  11. 動物⾏動学を⽤いた新規病態評価法の確⽴

    越智翔平

    新興・再興感染症研究基盤創生事業（海外拠点活用研究領域） 班会議―ガーナとザンビアを繋ぐアフリカ横断的ウイルス研究: 動物 行動から分子メカニズムまでの分野融合的アプローチ　2025/02/12

  12. 自閉症モデルマウスを用いた集団行動動態解析

    越智翔平

    第19回自閉症学研究会　2025/02/08

  13. ガーナとザンビアを繋ぐアフリカ横断的ウイルス研究: 動物行動から分子メカニズムまでの分野融合的アプローチ

    梶原将大, 板倉友香里, 山地俊之, 越智翔平

    新興・再興感染症研究基盤創生事業 (海外拠点活用研究領域) 令和6年度 海外拠点・海外拠点活用研究領域 研究交流会　2025/02/06

  14. RFIDとビジョンの特性を考慮した対応・欠損値同時推定による複数マウスのトラッキング

    千葉直也, 越智翔平, Matus Tanonwong, 大隅典子, 橋本浩一

    SICE SI 2024　2024/12/18

  15. 神経発達障害の病因・病態の理解に資する脳の性差のマルチモーダルな探求

    越智翔平

    科学研究費助成事業 学術変革領域研究(A)「階層的生物ナビ学」 領域対面イベント　2024/11/05

  16. 集団行動の⻑期追跡を用いたマウスにおける階層的インタラクションの理解

    越智翔平

    科学研究費助成事業 学術変革領域研究(A)「階層的生物ナビ学」 領域対面イベント　2024/11/05

  17. 神経発達障害の病因・病態の理解に資する脳の性差のマルチモーダル な探求

    大隅典子, 越智翔平

    AMED 精神・神経疾患メカニズム解明プロジェクト 領域横断的かつ萌芽的脳研究プロジェクト 第3回分科会　2024/09/13

  18. Elucidating sex disparities in gene expression in the embryonic cortex: insights from Pax6 mutant mice, a neurodevelopmental disorder model animal

    Shyu Manabe, Shohei Ochi, Noriko Osumi

    The 3rd Taiwan Society for Neuroscience Meeting　2024/08/25

  19. 集団飼育環境における自閉症モデルマウスの行動評価系の開発

    越智翔平

    第18回自閉症学研究会　2024/07/27

  20. The association between cytokine elevation induced by maternal immune activation and ASD-like phenotypes in Mouse offspring

    Hinako Kirikae, Florian Pans, Minori Ikuta, Erika Uchibori, Mai Sakai, Shohei Ochi, Noriko Osumi, Hiroaki Tomita, Motoko Maekawa, Yuji Owada, Yoshiyuki Kasahara

    NEURO2024　2024/07/24

  21. A novel system for long-term evaluation of social behavior and activity in Pax6Sey/+ male and female mice

    Sander van der Graaf, Shohei Ochi, Noriko Osumi

    NEURO2024　2024/07/24

  22. Analysis of a neurodevelopmental disorder-like mouse model in a large group-housing condition

    Shohei Ochi, Sander van der Graaf, Hitoshi Inada, Noriko Osumi

    NEURO2024　2024/07/24

  23. Unveiling sex differences in gene expression within the embryonic cortex: insights from Pax6 mutant mice

    Shyu Manabe, Shohei Ochi, Noriko Osumi

    15th KSMCB Korea-Japan Neurodevelopmental Biology Society Meeting　2024/07/05

  24. 集団行動の長期追跡を用いたマウスにおける階層的インタラクションの理解

    越智翔平

    科学研究費助成事業 学術変革領域研究（A）「階層的生物ナビ学」 2024年度領域会議　2024/06/25

  25. Automated analysis of rodent behavior using IntelliProfiller—A potential tool for studying neurodevelopmental disorder-like model mice

    Shohei Ochi, Sander van der Graaf, Hitoshi Inada, Noriko Osumi

    Cold Spring Harbor Asia Conference Autism & Neurodevelopment Disorders – from Genetic Discoveries to Interventions　2024/06/18

  26. Automated analysis of rodent behavior using IntelliProfiller: a potential tool for studying neurodevelopmental disorder-like model mice

    Shohei Ochi, Hitoshi Inada, Noriko Osumi

    CINP2024　2024/05/25

  27. Shedding light on the molecular mechanisms underlying sex difference in the pathogenesis of autism spectrum disorder

    Shyu Manabe, Shohei Ochi, Noriko Osumi

    2024/04/12

  28. Utilising a novel long-term behaviour evaluation system to assess social behaviour and activity in mutant Pax6 Sey/- male and female mice

    Sander van der Graaf, Shohei Ochi, Noriko Osumi

    2024/04/12

  29. マウス大脳発生における性差の解明への取り組み: 神経発達障害モデルPax6変異マウスから得られた知見

    眞鍋 柊, 越智 翔平, 大隅 典子

    第17回神経発生討論会 第20回成体脳のニューロン新生懇談会 合同大会　2024/03/09

  30. Unraveling the molecular mechanisms underlying sex differences in the mammalian corticogenesis: insights from a Pax6 mutant mouse model of neurodevelopmental disorder

    Shyu Manabe, Shohei Ochi, Noriko Osumi

    Society for Neuroscience 2023　2023/11/12

  31. Automated analysis of rodent behavior using IntelliProfiller: a potential tool for studying neurodevelopmental disorder-like model mice

    Shohei Ochi, Hitoshi Inada, Noriko Osumi

    SfN23　2023/11/12

  32. Analysis of a neurodevelopmental disorder-like mouse model in a posthabituated group-housing condition

    Shohei Ochi, Hitoshi Inada, Noriko Osumi

    The 53rd Annual Meeting of the Japanese Society of Neuropsychopharmacology　2023/09/08

  33. マウス大脳皮質原基における雌雄差を形成する分子メカニズムの解明

    眞鍋柊, 越智翔平, 大隅典子

    精神・神経疾患領域/早期ライフ連携推進ワークショップ　2023/08/29

  34. IntelliProfiler：新規社会行動評価系の開発および自閉症モデルマウスへの展開

    越智翔平, 稲田 仁, 大隅 典子

    精神・神経疾患領域/早期ライフ連携推進ワークショップ　2023/08/29

  35. Analyses of molecular profile between male and female during murine brain development

    Takako Kikkawa, Shyu Manabe, Sara Ebrahimiazar, Shohei Ochi, Noriko Osumi

    The 46th Annual Meeting of the Japan Neuroscience Society　2023/08/02

  36. Analysis towards the sex difference in the gene expression pattern during murine corticogenesis

    Shyu Manabe, Shohei Ochi, Noriko Osumi

    The 46th Annual Meeting of the Japan Neuroscience Society　2023/08/02

  37. Analysis of a neurodevelopmental disorder-like mouse model in a post-habituated group-housing condition

    Shohei Ochi, Shyu Manabe, Hitoshi Inada, Noriko Osumi

    The 46th Annual Meeting of the Japan Neuroscience Society　2023/08/01

  38. Sex differences of molecular program in the murine embryonic brain

    Takako Kikkawa, Shyu Manabe, Sara Ebrahimiazar, Shohei Ochi, Noriko Osumi

    The 64th Annual Meeting of the Japanese Society of Neuropathology The 66th Annual Meeting of the Japanese Society for Neurochemistry　2023/07/06

  39. Elucidating the molecular mechanism leading to the sexual differentiation of the murine cortical primordium

    Shyu Manabe, Shohei Ochi, Noriko Osumi

    第6回春の神経発生研究会　2023/04/03

  40. Elucidating the molecular mechanism leading to the sexual differentiation of the murine cortical primordium

    Shyu Manabe, Shohei Ochi, Noriko Osumi

    第128回日本解剖学会総会・全国学術集会　2023/03/19

  41. 大脳皮質形成過程におけるPax6 下流のδカテニンの発現に関する性差研究への取り組み

    越智 翔平, 眞鍋 柊, Seung Hee Chun, 吉川 貴子, 大隅 典子

    The 128th Annual Meeting of The Japanese Association of Anatomists　2023/03/19

  42. マウス集団飼育を用いた長期神経発達症様行動特徴の解析

    The 15th Japanese Consortium for Autism Research　2023/01/28

  43. IntelliProfiler system: Development of a novel social behavior evaluation system and its application to autism spectrum disorder model mouse

    Shohei Ochi, Hitoshi Inada, Noriko Osumi

    The 21st Takeda Science Foundation Symposium on Bioscience　2023/01/27

  44. マウス大脳皮質原基における雌雄差形成に関わる分子メカニズム解明への取り組み

    越智 翔平

    2022年度「先進ゲノム支援」拡大班会議　2023/01/19

  45. マウス大脳皮質原基における雌雄差を形成する分子メカニズムの解明

    眞鍋柊, 越智翔平, 大隅典子

    NGS発生生物学現場の会2022　2022/12/07

  46. 集団飼育環境における神経発達障害様行動特徴の解析

    越智 翔平, 稲田 仁, 大隅 典子

    第4回共調的社会脳研究会　2022/11/05

  47. IntelliBoardを用いた新規マウス社会行動評価システムの確立

    The 14th Japanese Consortium for Autism Research　2022/07/24

  48. Sex difference in the gene expression pattern during murine corticogenesis

    Shyu Manabe, Shohei Ochi, Takako Kikkawa, Noriko Osumi

    NEURO2022　2022/06/30

  49. IntelliBoard system: Development of a novel social behavior evaluation system and its application to Autism Spectrum Disorder model mouse

    Shohei Ochi, Hitoshi Inada, Noriko Osumi

    NEURO2022　2022/06/30

  50. The study towards understanding sex differences in the murine developing cerebral cortex

    Shyu Manabe, Seung Hee Chun, Shohei Ochi, Takako Kikkawa, Noriko Osumi

    第5回春の神経発生研究会　2022/04/11

  51. Understanding sex differences in the expression pattern of the δ- catenin, a downstream to Pax6, in the mammalian corticogenesis

    Shohei Ochi, Shyu Manabe, Seung Hee Chun, Kikkawa Takako, Noriko Osumi

    The 127th Annual Meeting of The Japanese Association of Anatomists　2022/03/28

  52. The study towards understanding sex differences in the murine developing cerebral cortex

    眞鍋柊, Seun Hee Chun, 越智翔平, 吉川貴子, 大隅典子

    第１５回神経発生討論会　2022/03/19

  53. Understanding sex differences in the expression pattern of the δ- catenin, a downstream to Pax6, in the mammalian corticogenesis

    Shohei Ochi, Shyu Manabe, Seung Hee Chun, Kikkawa Takako, Noriko Osumi

    2021/12/03

  54. 胎生期大脳皮質におけるPax6下流因子δカテニンの発現推移と雌雄差解析

    越智 翔平, 眞鍋 柊, Seung Hee Chun, 吉川 貴子, 大隅 典子

    日本解剖学会 第67回東北・北海道連合支部学術集会　2021/09/05

  55. The study towards understanding the expression patterns and the sexual differences of the δ-catenin, a downstream to Pax6, in the mammalian corticogenesis

    Shyu Manabe, Shohei Ochi, Seung Hee Chun, Takako Kikkawa, Noriko Osumi

    The 80th Fuihara Seminar Molecular and cellular mechanism of brain systems generating individuality　2021/08/30

  56. The analysis towards understanding sex differences in the murine developing cerebral cortex

    Shyu Manabe, Shohei Ochi, Takako Kikkawa, Noriko Osumi

    The 44th Annual Meeting of the Japanese Neuroscience Society The 1st CJK International Meeting　2021/07/31

  57. Analysis of sexual dimorphism in the developing mouse embryonic cerebral cortex

    Shohei Ochi

    The 14th Annual Meeting for Japanese Developmental Neuroscientists　2021/03/19

  58. マウス胎仔大脳皮質発生過程におけるPax6下流因子δカテニンの雌雄差解析

    眞鍋柊, 越智翔平, Seung Hee Chun, 吉川貴子, 大隅典子

    新学術領域研究 「個性」創発脳 第5回領域班会議　2020/12/19

  59. 胎生期マウス大脳皮質における脳構築の遺伝的プログラムの性差研究

    越智 翔平, 眞鍋 柊, 吉川 貴子, 大隅 典子

    新学術領域研究「個性」創発脳 第 5 回領域会議　2020/12/18

  60. 哺乳類の終脳初期発生過程におけるPax6下流因子δカテニンの機能解析

    眞鍋柊, 越智翔平, Seung Hee Chun, 吉川貴子, 大隅典子

    第43回日本分子生物学会年会　2020/12/02

  61. Hes1遺伝子の発現振動は胎生期の脳において細胞増殖や神経分化を制御する

    越智 翔平, 今泉 結, 下條 博美, 宮地 均, 影山 龍一郎

    第43回日本神経科学大会　2020/07/29

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Research Projects 10

  1. 集団飼育環境における自閉スペクトラム症モデルマウスの行動オミクス解析

    越智翔平

    Offer Organization: 武田科学振興財団

    System: 2024年度 武田科学振興財団 医学系研究助成(精神・神経・脳領域)

    Institution: 東北大学

    2024/07 - 2029/03

  2. Exploring shared molecular mechanisms between neurodevelopmental disorders and epilepsy.

    Hitoshi Inada, Shohei Ochi, Noboru Hiroi, Takeshi Hiramoto, Takeshi Takano

    Offer Organization: 自然科学研究機構

    System: 2025年度日米科学技術協力事業「脳研究」分野

    2025/04 - 2028/03

  3. ガーナとザンビアを繋ぐアフリカ横断的ウイルス研究: 動物行動から分子メカニズムまでの分野融合的アプローチ

    梶原将大, 板倉友香里, 山地俊之, 越智翔平

    Offer Organization: 国立研究開発法人日本医療研究開発機構

    System: 新興・再興感染症研究基盤創生事業（海外拠点活用研究領域）基礎的研究公募枠

    2024/12 - 2027/03

  4. 脆弱X症候群とプラダー・ウィリー症候群の間を繋ぐインプリンティング機構の解明

    吉川貴子, 越智翔平

    Offer Organization: 国立研究開発法人日本医療研究開発機構

    System: 令和6年度脳神経科学統合プログラム（個別重点研究課題）

    Institution: 東北大学

    2024/09 - 2027/03

  5. Understanding sex differences in behavior and underlying neurodevelopmental mechanisms using mouse models of neurodevelopmental disorders

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2024/04 - 2027/03

  6. 集団行動の長期追跡を用いたマウスにおける階層的インタラクションの理解

    越智 翔平

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 学術変革領域研究(A)

    Institution: 東北大学

    2024/04 - 2026/03

  7. 脳とこころの研究推進プログラム（精神・神経疾患メカニズム解明プロジェクト）

    大隅典子, 洲崎悦生, 越智翔平

    Offer Organization: 国立研究開発法人日本医療研究開発機構

    Category: 神経発達障害の病因・病態の理解に資する脳の性差のマルチモーダルな探求

    2021/04 - 2025/03

  8. 集団飼育環境における神経発達障害モデルマウスの包括的行動評価システムの開発

    越智翔平

    Offer Organization: 東北大学

    System: 令和6年度 東北大学大学院医学系研究科 若手共同研究奨励研究費

    Institution: 東北大学

    2024 - 2025

  9. Elucidation of the mechanisms of differential gene expression in the male and female mice on the sexual differentiation of the cerebral cortex

    Shohei Ochi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    Category: Grant-in-Aid for Early-Career Scientists

    Institution: Tohoku University

    2021/04 - 2023/03

  10. 神経発生過程におけるＨｅｓ１遺伝子の発現振動の意義の解明

    越智 翔平

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 京都大学

    2017/04/26 - 2019/03/31

    More details Close

    神経発生過程において、Hes1遺伝子は神経幹細胞の維持に重要な役割を担う。加えて、その発現は2-3時間の一定の周期で振動する。しかし、Hes1遺伝子の発現振動が神経分化にどのような役割をもつのかは未解明である。この問題を明らかにする手法として、Hes1遺伝子のイントロン配列を除去し、転写にかかる時間を短縮した。これによって、Hes1遺伝子の発現動態を振動発現から減衰振動に改変した。以上の手法により、発現振動が神経分化に与えている影響の解明を試みた。 Hes1遺伝子のイントロン配列を除去したノックインマウス（Hes1イントロン欠損マウス）を新たに作製した。Hes1イントロン欠損マウスでは、野生型マウスに比べ体のサイズが小さくなる傾向が認められた。細胞分裂・細胞死について観察したところ、Hes1イントロン欠損マウスでは、細胞分裂の数が少なくなり、細胞死に至る細胞の数が多くなる傾向が認められた。これらのことが起因となり、体のサイズを縮小化させている可能性がある。Hes1イントロン欠損マウスの脳では、Hes5遺伝子の発現量が増加していた。神経発生過程において、Hes5遺伝子は、Hes1遺伝子と同等の機能を担い補償的に作用することが知られている。そこで、Hes5遺伝子の相補的影響を排除するため、Hes1イントロン欠損マウスとHes5欠損マウスを掛け合わせた二重変異マウスを作製し、神経分化過程を解析した。二重変異マウスの大脳皮質において、基底側面における細胞分裂の数が増加していた。この結果は、中間前駆細胞の数が増大したことが示唆される。それに応じ、二重変異マウスでは、大脳皮質の深層ニューロン、浅層ニューロンの層が肥大化した。この結果は、神経分化のタイミングが加速化していることが示唆できる。 上記の結果より、Hes1遺伝子の発現振動自体が、神経幹細胞の増殖や神経分化のタイミングの制御に重要な機能を担っていることが明らかになった。

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Teaching Experience 3

  1. 発生学実習

  2. 発生学

  3. 脳科学講義Ⅲ／先進脳科学講義Ⅲ

Academic Activities 5

  1. 第48回 日本神経科学大会 座長

    2025/07/26 - 2025/07/26

  2. 第34回 神経行動薬理若手研究者の集い 座長

    2025/03/16 - 2025/03/16

  3. 第19回 自閉症学研究会 フラッシュトークセッション座長

    2025/02/08 - 2025/02/09

  4. NEURO2024, Training School for Next Generation Scientists, Young Chair

    2024/07/26 - 2024/07/26

    Activity type: Academic society, research group, etc.

  5. The 46th Annual Meeting of the Japan Neuroscience Society, Training School for Next Generation Scientists

    2023/08/01 - 2023/08/01

    Activity type: Academic society, research group, etc.