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Hiroaki Murakami

Section

Graduate School of Agricultural Science

Job title

Assistant Professor

Degree

博士（農学）（京都大学）
修士（生命科学）（京都大学）

researchmap

https://researchmap.jp/hiroaki_murakami

J-GLOBAL ID

202001011909038470

e-Rad No.

60880721

ORCID

https://orcid.org/0000-0003-0283-7106

Researcher ID

JHQ-3968-2023

Profile

Research History 4

2024/05 - Present

The Open University of Japan
2022/04 - Present

Tohoku University　Fisheries Biology and Ecology, Graduate School of Agricultural Science　Assistant Professor
2020/04 - 2022/03

Kyoto University　Field Science Education and Research Center, Maizuru Fisheries Research Station (MFRS))　Postdoctoral Fellow
2019/10 - 2020/03

Kyoto University　Field Science Education and Research Center

Professional Memberships 3

環境DNA学会

2018/11 - Present
The Ecological society of Japan

2016/03 - Present
日本水産学会

2016/03 - Present

Research Interests 3

魚類生態学
水産学
Environmental DNA

Research Areas 1

Life sciences / Ecology and environmental science / Environmental DNA

Awards 2

Paper Award

2022/03　Dispersion and degradation of environmental DNA from caged fish in a marine environment
Certificate for excellence in poster presentation

2013/12　The 12th NTU-Japan International Student Mini-Symposium on Life Sciences at National Taiwan University　Genetic analysis of a mechanism to maintain proper distribution of CENP-A

Papers 19

Cdc48 and its co-factor Ufd1 extract CENP-A from centromeric chromatin and can induce chromosome elimination in the fission yeast Schizosaccharomyces pombe Peer-reviewed

Yukiko Nakase, Hiroaki Murakami, Michiko Suma, Kaho Nagano, Airi Wakuda, Teppei Kitagawa, Tomohiro Matsumoto

Biology Open　2024/03/25
Publisher: The Company of Biologists
DOI： 10.1242/bio.060287 　

eISSN： 2046-6390

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CENP-A determines the identity of the centromere. Because the position and size of the centromere and its number per chromosome must be maintained, the distribution of CENP-A is strictly regulated. In this study, we have aimed to understand mechanisms to regulate the distribution of CENP-A (Cnp1SP) in fission yeast. A mutant of the ufd1+ gene (ufd1-73) encoding a cofactor of Cdc48 ATPase is sensitive to Cnp1 expressed at a high level and allows mislocalization of Cnp1. The level of Cnp1 in centromeric chromatin is increased in the ufd1-73 mutant even when Cnp1 is expressed at a normal level. A preexisting mutant of the cdc48+ gene (cdc48-353) phenocopies the ufd1-73 mutant. We have also shown that Cdc48 and Ufd1 proteins physically interact with centromeric chromatin. Finally, Cdc48 ATPase with Ufd1 artificially recruited to the centromere of a mini-chromosome (Ch16) induce a loss of Cnp1 from Ch16, leading to an increased rate of chromosome loss. It appears that Cdc48 ATPase, together with its cofactor Ufd1 remove excess Cnp1 from chromatin, likely in a direct manner. This mechanism may play a role in centromere disassembly, a process to eliminate Cnp1 to inactivate the kinetochore function during development, differentiation, and stress response.
Analysis of the Persistence and Particle Size Distributional Shift of Sperm-Derived Environmental DNA to Monitor Jack Mackerel Spawning Activity Peer-reviewed

Satsuki Tsuji, Hiroaki Murakami, Reiji Masuda

Environmental Science & Technology　2022/07/22
Publisher: American Chemical Society (ACS)
DOI： 10.1021/acs.est.2c01904 　

ISSN： 0013-936X

eISSN： 1520-5851
Dispersion and degradation of environmental DNA from caged fish in a marine environment

弘章村上, 錫鎭尹, 亮秀笠井, 利文源, 哲史山本, 雅之坂田, 智矢堀内, 英樹澤田, 倫生近藤, 洋山下, 玲爾益田

NIPPON SUISAN GAKKAISHI　88　(4)　206-206　2022/07/15
Publisher: Japanese Society of Fisheries Science
DOI： 10.2331/suisan.133 　

ISSN： 0021-5392

eISSN： 1349-998X
An efficient early‐pooling protocol for environmental <scp>DNA</scp> metabarcoding Peer-reviewed

Masayuki Ushio, Saori Furukawa, Hiroaki Murakami, Reiji Masuda, Atsushi J. Nagano

Environmental DNA　2022/07/05
Publisher: Wiley
DOI： 10.1002/edn3.337 　

ISSN： 2637-4943

eISSN： 2637-4943
Seasonal changes in the distribution of black sea bream Acanthopagrus schlegelii estimated by environmental DNA Peer-reviewed

Sachia Sasano, Hiroaki Murakami, Keita W. Suzuki, Toshifumi Minamoto, Yoh Yamashita, Reiji Masuda

Fisheries Science　88　(1)　91-107　2022/01/30
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s12562-021-01572-z 　

ISSN： 0919-9268

eISSN： 1444-2906
Environmental DNA emission by two carangid fishes in single and mixed-species tanks Peer-reviewed

Hiroaki Murakami, Reiji Masuda, Satoshi Yamamoto, Toshifumi Minamoto, Yoh Yamashita

Fisheries Science　88　(1)　55-62　2022/01
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s12562-021-01565-y 　

ISSN： 0919-9268

eISSN： 1444-2906
Universal performance of benzalkonium chloride for the preservation of environmental DNA in seawater samples Peer-reviewed

Toshiaki Jo, Masayuki K. Sakata, Hiroaki Murakami, Reiji Masuda, Toshifumi Minamoto

Limnology and Oceanography: Methods　19　(11)　758-768　2021/11
Publisher: Wiley
DOI： 10.1002/lom3.10459 　

ISSN： 1541-5856

eISSN： 1541-5856
Estimating fish population abundance by integrating quantitative data on environmental DNA and hydrodynamic modelling Peer-reviewed

Keiichi Fukaya, Hiroaki Murakami, Seokjin Yoon, Kenji Minami, Yutaka Osada, Satoshi Yamamoto, Reiji Masuda, Akihide Kasai, Kazushi Miyashita, Toshifumi Minamoto, Michio Kondoh

Molecular Ecology　30　(13)　3057-3067　2021/07
Publisher: Wiley
DOI： 10.1111/mec.15530 　

ISSN： 0962-1083

eISSN： 1365-294X
Selective collection of long fragments of environmental DNA using larger pore size filter Peer-reviewed

Toshiaki Jo, Hiroaki Murakami, Reiji Masuda, Toshifumi Minamoto

Science of the Total Environment　(in press)　2020/05
Estimating shedding and decay rates of environmental nuclear DNA with relation to water temperature and biomass Peer-reviewed

Toshiaki Jo Mio Arimoto Hiroaki Murakami Reiji Masuda Toshifumi Minamoto

2019/11
Particle Size Distribution of Environmental DNA from the Nuclei of Marine Fish Peer-reviewed

Toshiaki Jo, Mio Arimoto, Hiroaki Murakami, Reiji Masuda, Toshifumi Minamoto

ENVIRONMENTAL SCIENCE & TECHNOLOGY　53　(16)　9947-9956　2019/08
Publisher: AMER CHEMICAL SOC
DOI： 10.1021/acs.est.9b02833 　

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Environmental DNA (eDNA) analyses have enabled a more efficient surveillance of species distribution and composition than conventional methods. However, the characteristics and dynamics of eDNA (e.g., origin, state, transport, and fate) remain unknown. This is especially limited for the eDNA derived from nuclei (nu-eDNA), which has recently been used in eDNA analyses. Here, we compared the particle size distribution (PSD) of nu-eDNA from Japanese Jack Mackerel (Trachurus japonicus) with that of mt-eDNA (eDNA derived from mitochondria) reported in previous studies. We repeatedly sampled rearing water from the tanks under multiple temperatures and fish biomass levels, and quantified the copy numbers of size-fractioned nu-eDNA. We found that the concentration of nu-eDNA was higher than that of mt-eDNA at 3-10 mu m size fraction. Moreover, at the 0.8-3 mu m and 0.4-0.8 mu m size fractions, eDNA concentrations of both types increased with higher temperature and their degradation tended to be suppressed. These results imply that the production of eDNA from large to small size fractions could buffer the degradation of small-sized eDNA, which could improve its persistence in water. Our findings will contribute to refine the difference between nu- and mt-eDNA properties, and assist eDNA analyses as an efficient tool for the conservation of aquatic species.
Biomass‐dependent emission of environmental DNA in jack mackerel Trachurus japonicus juveniles Peer-reviewed

Tomoya Horiuchi, Reiji Masuda, Hiroaki Murakami, Satoshi Yamamoto, Toshifumi Minamoto

Journal of Fish Biology　2019/07/31
Publisher: Wiley
DOI： 10.1111/jfb.14095 　

ISSN： 0022-1112

eISSN： 1095-8649
Effect of water temperature and fish biomass on environmental DNA shedding, degradation, and size distribution Peer-reviewed

Toshiaki Jo, Hiroaki Murakami, Satoshi Yamamoto, Reiji Masuda, Toshifumi Minamoto

ECOLOGY AND EVOLUTION　9　(3)　1135-1146　2019/02
Publisher: WILEY
DOI： 10.1002/ece3.4802 　

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Environmental DNA (eDNA) analysis has successfully detected organisms in various aquatic environments. However, there is little basic information on eDNA, including the eDNA shedding and degradation processes. This study focused on water temperature and fish biomass and showed that eDNA shedding, degradation, and size distribution varied depending on water temperature and fish biomass. The tank experiments consisted of four temperature levels and three fish biomass levels. The total eDNA and size-fractioned eDNA from Japanese Jack Mackerels (Trachurus japonicus) were quantified before and after removing the fish. The results showed that the eDNA shedding rate increased at higher water temperature and larger fish biomass, and the eDNA decay rate also increased at higher temperature and fish biomass. In addition, the small-sized eDNA fractions were proportionally larger at higher temperatures, and these proportions varied among fish biomass. After removing the fish from the tanks, the percentage of eDNA temporally decreased when the eDNA size fraction was >10 mu m, while the smaller size fractions increased. These results have the potential to make the use of eDNA analysis more widespread in the future.
Dispersion and degradation of environmental DNA from caged fish in a marine environment

Hiroaki Murakami, Seokjin Yoon, Akihide Kasai, Toshifumi Minamoto, Satoshi Yamamoto, Masayuki K. Sakata, Tomoya Horiuchi, Hideki Sawada, Michio Kondoh, Yoh Yamashita, Reiji Masuda

Fisheries Science　85　(2)　327-337　2019/01/03
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s12562-018-1282-6 　

ISSN： 0919-9268

eISSN： 1444-2906

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Abstract Environmental DNA (eDNA) consists of DNA fragments shed from organisms into the environment, and can be used to identify species presence and abundance. This study aimed to reveal the dispersion and degradation processes of eDNA in the sea. Caged fish were set off the end of a pier in Maizuru Bay, the Sea of Japan, and their eDNA was traced at sampling stations located at the cage and 10, 30, 100, 300, 600 and 1000 m distances from the cage along two transect lines. Sea surface water was collected at each station at 0, 2, 4, 8, 24 and 48 h after setting the cage, and again after removing the cage. Quantitative PCR analyses using a species-specific primer and probe set revealed that the target DNA was detectable while the cage was present and for up to 1 h after removing the cage, but not at 2 h or later. Among the 57 amplified samples, 45 (79%) were collected within 30 m from the cage. These results suggest that eDNA can provide a snapshot of organisms present in a coastal marine environment.
Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing Peer-reviewed

Masayuki Ushio, Hiroaki Murakami, Reiji Masuda, Tetsuya Sado, Masaki Miya, Sho Sakurai, Hiroki Yamanaka, Toshifumi Minamoto, Michio Kondoh

Metabarcoding and Metagenomics (MBMG)　2017/11
Rapid degradation of longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA Peer-reviewed

Toshiaki Jo, Hiroaki Murakami, Reiji Masuda, Masayuki K. Sakata, Satoshi Yamamoto, Toshifumi Minamoto

MOLECULAR ECOLOGY RESOURCES　17　(6)　e25-e33　2017/11
Publisher: WILEY
DOI： 10.1111/1755-0998.12685 　

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The advent of environmental DNA (eDNA) analysis methods has enabled rapid and wide-range ecological monitoring in aquatic ecosystems, but there is a dearth of information on eDNA degradation. The results of previous studies suggest that the decay rate of eDNA varies depending on the length of DNA fragments. To examine this hypothesis, we compared temporal change in copy number of long eDNA fragments (719bp) with that of short eDNA fragments (127bp). First, we isolated rearing water from a target fish species, Japanese Jack Mackerel (Trachurus japonicus), and then quantified the copy number of the long and short eDNA fragments in 1L water samples after isolating the water from the fish. Long DNA fragments showed a higher decay rate than short fragments. Next, we measured the eDNA copy numbers of long and short DNA fragments using field samples, and compared them with fish biomass as measured by echo intensity. Although a previous study suggested that short eDNA fragments could be overestimated because of nontarget eDNA from a nearby fish market and carcasses, the eDNA concentrations of long fragments were correlated with echo intensity. This suggests that the concentration of longer eDNA fragments reflects fish biomass more accurately than the previous study by removing the effects of the fish market and carcasses. The length-related differences in eDNA have a substantial potential to improve estimation of species biomass.
Inconsistency between salinity preference and habitat salinity in euryhaline gobiid fishes in the Isazu River, northern Kyoto Prefecture Peer-reviewed

Yumeki Oto, Masahiro Nakamura, Hiroaki Murakami, Reiji Masuda

JOURNAL OF ETHOLOGY　35　(2)　203-211　2017/05
Publisher: SPRINGER JAPAN KK
DOI： 10.1007/s10164-017-0510-3 　

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Adults of three amphidromous gobiid fishes, Tridentiger brevispinis, Rhinogobius similis, and Gymnogobius petschiliensis, are euryhaline and generally found in both freshwater (FW) and brackish water (BW) areas. The determining factors for their choice of habitat with different salinity have never been explored. In this study, a salinity-choice experiment was conducted using the above species captured in the FW region of the Isazu River, northern Kyoto Prefecture. For comparison, the fluvial goby Rhinogobius flumineus and BW-acclimated G. petschiliensis were also tested. We found that the three euryhaline species, including BW-acclimated G. petschiliensis, preferred BW to FW, whereas R. flumineus preferred FW. These results suggest that salinity preference did not determine habitat in these euryhaline gobiids, which were found in FW. Surveys were also conducted focusing on competitors and predators in their potential habitats. Thus, net sampling captured many other gobiid species, and an environmental DNA method detected Japanese temperate bass, a voracious predator, in the estuarine areas, suggesting that biotic factors are major determinants in the distribution of euryhaline species.
Environmental DNA as a 'Snapshot' of Fish Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan (vol 11, e0149786, 2016) Peer-reviewed

Satoshi Yamamoto, Kenji Minami, Keiichi Fukaya, Kohji Takahashi, Hideki Sawada, Hiroaki Murakami, Satsuki Tsuji, Hiroki Hashizume, Shou Kubonaga, Tomoya Horiuchi, Masamichi Hongo, Jo Nishida, Yuta Okugawa, Ayaka Fujiwara, Miho Fukuda, Shunsuke Hidaka, Keita W. Suzuki, Masaki Miya, Hitoshi Araki, Hiroki Yamanaka, Atsushi Maruyama, Kazushi Miyashita, Reiji Masuda, Toshifumi Minamoto, Michio Kondoh

PLOS ONE　11　(4)　2016/04

DOI： 10.1371/journal.pone.0153291 　

ISSN： 1932-6203
Environmental DNA as a 'Snapshot' of Fish Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan Peer-reviewed

Satoshi Yamamoto, Kenji Minami, Keiichi Fukaya, Kohji Takahashi, Hideki Sawada, Hiroaki Murakami, Satsuki Tsuji, Hiroki Hashizume, Shou Kubonaga, Tomoya Horiuchi, Masamichi Hongo, Jo Nishida, Yuta Okugawa, Ayaka Fujiwara, Miho Fukuda, Shunsuke Hidaka, Keita W. Suzuki, Masaki Miya, Hitoshi Araki, Hiroki Yamanaka, Atsushi Maruyama, Kazushi Miyashita, Reiji Masuda, Toshifumi Minamoto, Michio Kondoh

PLOS ONE　11　(3)　e0149786　2016/03

DOI： 10.1371/journal.pone.0149786 　

ISSN： 1932-6203

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Misc. 18

舞鶴湾におけるマアジとカタクチイワシの環境DNAの水平・鉛直分布口頭

村上弘章, 山本哲史, 源利文, 南憲吏, 宮下和士, 深谷肇一, 尹錫鎭, 笠井亮秀, 澤田英樹, 鈴木啓太, 益田玲爾, 山下洋, 近藤倫生

平成30年度日本水産学会春季大会　p.28　2018/03/27
異なるハビタットにおけるスズキ稚魚の生息密度と環境DNAの関係

村上弘章, 曽我部共生, 鈴木勇人, 源利文, 笠井亮秀, 鈴木啓太, 山下洋, 益田玲爾

2017年日本水産学会春季大会　p.33　2017/03/28
沿岸海域における環境DNAの分散過程に関する生簀を用いた検証実験

村上弘章, 尹錫鎭, 笠井亮秀, 源利文, 山本哲史, 坂田雅之, 堀内智矢, 澤田英樹, 益田玲爾

日本水産学会大会講演要旨集　2016　35　2016/03/26
音響テレメトリー手法を用いたスズキの河川に対する固執性と回帰に関する研究

眞名野将大, 眞名野将大, 久米学, 三田村啓理, 村上弘章, 寺島佑樹, 渡邊俊, 和田敏裕, 高木淳一, 市川光太郎, 山下洋

日本水産学会大会講演要旨集(CD-ROM)　2024　2024
スズキの河川利用と成長の地理的変異

高井万葉, 黒木真理, 白井厚太朗, 久米学, 寺島佑樹, 村上弘章, 冨樫博幸, 三田村啓理, 山下洋

日本水産学会大会講演要旨集(CD-ROM)　2024　2024
スズキ成魚が河川に回遊する理由

山下洋, 高井万葉, 黒木真理, 眞名野将大, 村上弘章, 目戸綾乃, 渡邊俊, 久米学, 寺島佑樹, 和田敏裕, 高木淳一, 市川光太郎, 三田村啓理

日本水産学会大会講演要旨集(CD-ROM)　2024　2024
階層的クラスタリング分析を用いたスズキの回遊パターンの分類

高井万葉, 黒木真理, 白井厚太朗, 寺島佑樹, 村上弘章, 久米学, 三田村啓理, 山下洋

日本水産学会大会講演要旨集(CD-ROM)　2024　2024
ホシガレイVerasper variegatusの環境DNA技術の確立と種苗放流後の分布推定

村上弘章, 深野直孝, う倩倩, 源利文, 山野辺貴寛, 高木淳一, 久米学, 山下洋, 三田村啓理, 和田敏裕

日本水産学会大会講演要旨集(CD-ROM)　2023　2023
瀬戸内海における河川の魚類多様性に与える環境要因と人間活動の影響

山崎彩, 村上弘章, 亀岡大真, 山中裕樹, 亀山哲, 久米学, 山下洋, 笠井亮秀

日本水産学会大会講演要旨集(CD-ROM)　2022　2022
Dispersion and degradation of environmental DNA from caged fish in a marine environment (vol 85, pg 327, 2019)

Hiroaki Murakami, Seokjin Yoon, Akihide Kasai, Toshifumi Minamoto, Satoshi Yamamoto, Masayuki K. Sakata, Tomoya Horiuchi, Hideki Sawada, Michio Kondoh, Yoh Yamashita, Reiji Masuda

FISHERIES SCIENCE　85　(6)　1109-1109　2019/11

DOI： 10.1007/s12562-019-01341-z 　

ISSN： 0919-9268

eISSN： 1444-2906
環境DNAから推測されるクロダイの分布様式：海洋および河川域での季節変化ポスター

笹野祥愛, 村上弘章, 鈴木啓太, 源利文, 山下洋, 益田玲爾

第1回環境DNA学会　2018/09/29
環境DNAが示すクロダイの河川進入の季節変化

笹野祥愛, 村上弘章, 山下洋, 益田玲爾

平成30年度日本水産学会秋季大会　p.9　2018/09
環境DNAを用いて調査したクロダイの沿岸依存性と産卵期・仔魚期の分布特性口頭

笹野祥愛, 村上弘章, 鈴木啓太, 源利文, 山下洋, 益田玲爾

平成30年度日本水産学会春季大会　p.47　2018/03/29
Evaluating the efficiency of environmental DNA through tnak experiments and field surveys Invited

MASUDA Reiji, MURAKAMI Hiroaki, TAKAHASHI Kohji, MINAMOTO Toshifumi, MIYA Masaki

Aquabiology　40　(1)　17-22　2018/02
大量シーケンスと標準DNAを利用した魚類環境DNAの網羅的・定量的モニタリング:京都府舞鶴湾での解析事例

潮雅之, 潮雅之, 村上弘章, 益田玲爾, 佐土哲也, 宮正樹, 櫻井翔, 山中裕樹, 源利文, 近藤倫生

水産海洋研究　81　(4)　305‐307　2017/11/10

ISSN： 0916-1562
堆積物からのマアジの環境DNAの検出: 水槽実験とフィールドでの検証

尾形瑞紀, 益田玲爾, 村上弘章, 余田昂彌, 澤田英樹, 山下洋, 源利文

2017年日本水産学会春季大会　p.34　2017/03/28
環境DNAを用いたクラゲ防除技術の開発：季節的モニタリングとポリプからの検出

余田昂彌, 益田玲爾, 村上弘章, 尾形瑞紀, 山下洋, 源利文

2017年日本水産学会春季大会　p.34　2017/03/28
日本海側河川の両側回遊性ハゼ科魚類における塩分環境利用パターンとこれに関わる環境要因

大戸夢木, 中村政裕, 村上弘章, 益田玲爾

日本水産学会大会講演要旨集　2016　2016

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Books and Other Publications 4

沿岸フィールド科学

山下洋, 益田玲爾, 甲斐嘉晃ら

2022/10
環境DNA学会ニュースレター no. 3

村上弘章, 学際融合教育研究推進センター, 舞鶴水産実験所, 特定研究員

2021/01
海洋における環境DNA研究の現状と今後の展望

Journal of Coastal Zone Studies　2020/06
環境DNAが拓く魚類生態研究の未来

益田玲爾・村上弘章・高橋宏司・源利文・宮正樹

海洋と生物　2018/02

Presentations 17

ホシガレイ Verasper variegatusの環境DNA技術の確立と種苗放流後の分布推定

村上弘章, 深野直孝, 鄔倩倩, 源利文, 髙木淳一, 久米学, 山下洋, 三田村啓理, 和田敏裕

令和５年度・日本水産学会秋季大会　2023/09/21
環境DNAメタバーコーディング解析で読み解く京都府由良川の魚類相の季節変化

村上弘章 (東北大農), 笹野祥愛 (京大フィールド研・現水産機構), 益田玲爾 (京大フィールド研), 山下洋 (京大フィールド研), 笠井亮秀 (北大院水産)

環境DNA学会「あなたが主役のワークショップ. 2022」　2022/11/19
Substantial seasonal intrusion of marine fish species elucidated by environmental DNA metabarcoding in Yura River, Kyoto

Hiroaki Murakami, Sachia Sasano, Reiji Masuda, Yoh Yamashita, Akihide Kasai

Tohoku University – OIST 3rd Joint Workshop on Biodiversity: From Genes and Species to Ecosystem Services and Resilience　2022/10/25
Distribution and seasonal migration of Japanese temperate bass Lateolabrax japonicus in Yura River detected by environmental DNA

2021/11/20
PicSeaってなんだ、シチズンサイエンスってなんだ?

村上弘章, 伊藤真, 伊勢武史

みんなのカメラで海洋ゴミの今を知る「PicSea」プロジェクトスタート記念オンライントークイベント　2021/09/25
環境DNAで読み解く河川と沿岸域の魚類多様性とその生態～シチズンサイエンスの実例～

村上弘章, 学際融合研, 内藤隆慈, 志水正敏, 北高校, 燈心嶺, 益田玲爾, 大フィールド研, 笠井亮秀

日本生態学会 ESJ68 自由集会 W18「自然を知り環境を守るための新技術・新発想」　2021/03/21
The influence of marine fish mixed with other species on environmental DNA emission

MURAKAMI Hiroaki, yoto, MASUDA Reiji, Kyoto Uni, YAMAMOTO Satoshi, Kyoto Univ, MINAMOTO Toshifumi, Kobe Univ, YAMASHITA Yoh

The 3rd Annual Meeting of The eDNA Society The 36th Annual Meeting of the Society of Population Ecology　2020/11/14
舞鶴湾におけるマアジ環境DNA濃度の日周変化

村上弘章・益田玲爾・山下洋

令和元年度第2回環境DNA学会　2019/11
環境DNAで読み解く舞鶴湾の魚の生態:スズキ、マアジ、カタクチイワシはどこにいる？ Invited

村上弘章

若狭湾生物同好会研修会　2018/06/16
舞鶴湾におけるマアジとカタクチイワシの環境DNA量と水平・鉛直方向の分布特性

村上弘章・源利文・山本哲史・近藤倫生・澤田英樹・山下洋・益田玲爾

平成30年度日本水産学会春季大会　2018/03
異なるハビタットにおけるスズキ稚魚の生息密度と環境DNAの関係

村上弘章・曽我部共生・鈴木勇人・源利文・笠井亮秀・鈴木啓太・山下洋・益田玲爾

平成29年度日本水産学会春季大会　2017/03
沿岸海域における環境DNAの分散過程に関する生簀を用いた検証実験

村上弘章・尹錫鎭・笠井亮秀・源利文・山本哲史・坂田雅之・堀内智矢・澤田英樹・益田玲爾

平成28年度日本水産学会春季大会　2016/03
瀬戸内海における河川の魚類多様性に与える環境要因と人間活動の影響

山崎彩, 村上弘章, 亀岡大真, 学際融合研セ, 山中裕樹, 亀山哲, 国環研, 久米学, 山下洋, 京大フィールド研セ, 笠井亮秀

令和3年度日本水産学会春季大会　2022/03/26
由良川に遡上するスズキの河川利用と成長

高井万葉, 黒木真理, 白井厚太朗, 眞名野将大, 目戸綾乃, 児嶋大地, 院情報, 寺島佑樹, 寺島環境, コンサル, 渡邊俊, 近, 和田敏裕, 村上弘章, 久米学, 市川光太郎, 澤田英樹, 三田村啓理, 山下洋, 京大フィールド研セ

令和3年度日本水産学会春季大会　2022/03/26
スズキの再生産における河川生物生産の貢献 –硫黄安定同位体比分析による推定

目戸綾乃, 眞名野将大, 渡邊俊, 鈴木啓太, 村上弘章, 寺島佑樹, 久米学, 市川光太郎, 和田敏裕, 児嶋大地, 大手信人, 三田村啓理

第11回同位体環境学シンポジウム　2021/12/17
丹後海・由良川におけるスズキの通し回遊

渡邊俊, 近大農, 眞名野将大, 目戸綾乃, 寺島佑樹, 京大フィールド研セ, 村上弘章, 学際融合研セ, 久米学, 三田村啓理, 市川光太郎, 鈴木啓太, 京大フィールド研セ, 蒋薇(京大院農, Edouard Lavergne, 河上哲生, 川上達也, 和田敏裕, 山下洋, 京大フィールド研セ

令和3年度日本水産学会春季大会　2021/03/27
The effect of body size and activity on eDNA in red-spotted grouper Epinephelus akaara

The 3rd Annual Meeting of The eDNA Society The 36th Annual Meeting of the Society of Population Ecology　2020/11/14

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Research Projects 17

環境DNAを用いた海産魚の遺伝的多様性評価技術の確立

村上弘章, 中臺亮介, 相馬(徐)寿明

Offer Organization: 一般社団法人ヤンマー資源循環支援機構

System: R6年度研究助成（一般）

Institution: 東北大学大学院農学研究科

2024/04 - 2026/04
日本における博士人材の育成環境をシリアスゲームで考える

打田篤彦, 伊藤真, 村上弘章

Offer Organization: 公益財団法人科学技術融合振興財団

System: 2023年度補助金助成

2024/01 - 2026/02
震災後に建設された護岸の種類が魚類の遺伝的多様性に与える影響

村上弘章,中臺亮介,相馬寿明,益田玲爾,横山勝英

Offer Organization: クリタ水・環境科学振興財団

System: R6年度研究助成金

Category: 国内研究・自然科学・技術（２）

Institution: 国立大学法人東北大学大学院農学研究科

2024/10 - 2025/09
生物資源量の把握と適正利用に向けた知の導出（研究開発課題-7）

村上弘章, 片山知史

Offer Organization: 科学技術振興機構（JST）

System: JST 共創の場形成支援プログラム（COI-NEXT）地域共創分野（本格型）「美食地政学に基づくグリーンジョブマーケットの醸成共創拠点」

Category: 課題リーダー

Institution: 国立大学法人東北大学

2025/04 -
気仙沼市西舞根川のフレーム護岸がニホンウナギ Anguilla japonicaの有効な生息場であるかの検証

村上弘章, 小林寛太, 益田玲爾, 横山勝英

Offer Organization: 一般財団法人自然環境研究センター

System: R6年度公益信託増進会自然環境保全研究活動助成基金

Institution: 国立大学法人東北大学大学院農学研究科

2024/07 - 2025/03
浜名湖のノコギリガザミの捕食生物に関する研究

片山知史・村上弘章・鈴木晶子

Offer Organization: 静岡県水産・海洋技術研究所

Institution: 国立大学法人東北大学大学院農学研究科、静岡県水産・海洋技術研究所

2024/07 - 2025/03
高解像度環境DNA分析による琵琶湖産魚類の種内・種間多様性の駆動プロセスの解明

中臺亮介, 相馬寿明, 村上弘章

Offer Organization: 京都大学生態学研究センター

System: R6年度・共同研究a

2024/04 - 2025/03
研究成果展開事業共創の場形成支援プログラム令和5年度次世代を担う人材育成支援

村上弘章, 乙木百合香, Oscar Tiku, 内田正紀, 田村綾海, 甲田紫乃

Offer Organization: 科学技術振興機構（JST）

System: JST 共創の場形成支援プログラム（COI-NEXT）地域共創分野（本格型）「美食地政学に基づくグリーンジョブマーケットの醸成共創拠点」

Institution: 国立大学法人東北大学

2023/11 - 2025/03
環境DNAを用いた只見町の奥地における魚類多様性評価

村上弘章, 深野直孝, 春本宜範, 片山知史

Offer Organization: 福島県只見町

System: 福島県只見町令和6年度「自然首都・只見」学術調査研究助成金

2023/06 - 2025/03
生物多様性ビッグデータの持続的創出に資する環境 DNA 分析手法の高度化

中臺亮介, 村上弘章, 徐寿明

Offer Organization: 独立行政法人環境再生保全機構

System: 令和5年度環境研究総合推進費

2023 - 2025
塩性湿地の創出を基軸とした災害に強い森里海まちづくり

横山勝英, 益田玲爾, 村上弘章他

Offer Organization: 公益財団法人日本生命財団

System: 学際的総合研究助成

2022/10 - 2024/10
環境DNAメタバーコーディング解析で読み解く只見町の魚類多様性

村上弘章, 深野直孝, 春本宜範, 城倉昴, 片山知史

Offer Organization: 福島県只見町

System: 令和５年度「自然首都・只見」学術調査研究助成金

Institution: 東北大学

2023/06 - 2024/03
美食地政学に基づくグリーンジョブマーケットの醸成共創拠点

松八重一代, 三橋正枝, 片山知史, 藤井豊展, 村上弘章他

Offer Organization: JST

System: JST共創の場形成支援プログラム（COI-NEXT）地域共創分野

Category: 農林水産資源の適切な管理と環境保全

Institution: 東北大学

2023/04 -
ICTインフラを用いた効果的な種苗放流による資源の安定化

和田敏裕, 三田村啓理, 久米学, 村上弘章, 山下洋他

Offer Organization: 農林水産省

System: 農林水産分野の先端技術展開事業のうち現地実証研究委託事業

2023/04 -
Development of environmental DNA technology for estimating the distribution of marine fishes and monitoring its seasonal changes

Hiroaki Murakami

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Early-Career Scientists

Institution: Kyoto University

2021/04/01 - 2023/03/31
ネイチャーポジティブ成長実現社会拠点

近藤倫生, 片山知史, 村上弘章他

Offer Organization: JST

System: 共創の場形成支援プログラム（COI-NEXT）【共創分野】

Institution: 東北大学

2023/03 -
環境DNAを用いた海産魚類の資源量および多様性の推定技術の確立

村上弘章

Offer Organization: 笹川科学研究助成

2016/04 - 2017/02

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Social Activities 7

環境DNAを用いた高知県吉野川と早明浦ダムの魚類相調査

2020/08/05 - 2020/08/06
RE:CONNECTウィークス

2022/02/20 -
舞鶴市の小学生向けの京都大学舞鶴水産実験所の施設見学と地Qクイズイベント

2021/10/22 -
舞鶴市おさかな観察会とRE:CONNECT地Qクイズの出題

2021/10/17 -
The relationship between echo intensity of a sonar and environmental DNA of jack mackerel and anchovy in Maizuru Bay

2021/02/17 -
嶺北高校生による吉野川水系の魚種調査共有会

2020/11/16 -
舞鶴市の小学生の京都大学舞鶴水産実験所施設見学と釣り体験

2020/09/27 -

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