TOHOKU UNIVERSITY Researchers

Details of the Researcher

Home

日本語 English

Shuichi Nakamura

Section

Graduate School of Engineering

Job title

Associate Professor

Degree

博士（理学）（大阪大学）
修士（農学）（茨城大学）

researchmap

https://researchmap.jp/shuichinakamura

J-GLOBAL ID

201201056021866155

Research History 4

2021/05 - Present

Tohoku University　Graduate School of Engineering Department of Applied Physics Applied Material Science Biophysical Engineering　Associate Professor
2010/12 - 2021/04

東北大学大学院工学研究科応用物理学専攻応用材料物理学講座生物物理工学分野　助教
2010/04 - 2010/11

大阪大学　グローバルCOE特任研究員
2009/04 - 2010/03

大阪大学　日本学術振興会特別研究員（PD）

Education 1

Osaka University　生命機能研究科　ナノ生体科学専攻

- 2009/03

Committee Memberships 6

日本生物物理学会　分野別専門委員

2022/04 - Present
Scientific Reports, Editorial Board Member (Biological Physics)

2022 - Present
Frontiers in Microbiology, Editorial Board Member (Microbial Physiology and Metabolism)

2021 - Present
日本生物物理学会　分野別専門委員

2019/04 - Present
日本細菌学会　東北支部地方委員

2018/04 - Present
BMC Microbiology, Editorial Board Member (Signaling and cellular microbiology)

2016 - Present

Show all ︎Show first 5

Professional Memberships 3

日本熱帯医学会

2021 - Present
日本細菌学会
日本生物物理学会

Research Interests 6

zoonosis
Salmonella
bio-imaging
spirochetosis
Host-Microbe interaction
Bacterial motility

Research Areas 3

Life sciences / Bacteriology /
Life sciences / Veterinary medicine /
Life sciences / Biophysics /

Awards 7

第 2 回「生命の情報物理学」領域賞

2022　新学術領域研究「情報物理学でひもとく生命の秩序と設計原理」　細菌の遊泳を加速する光活性型アデニル酸シクラーゼの発見
Cellular Microbiology Editor's Choice

2021　Cellular Microbiology
2019 Top cited article & Editor's Choice

2020/08　Biomolecules　Flagella-driven motility of bacteria
第8回Biophysics and Physicobiology論文賞

2019/09　日本生物物理学会
黒屋奨学賞

2019/04　日本細菌学会　細菌の運動機構に関する研究
Editor's choice

2017/02　Microbiology Society
日本生物物理学会若手奨励賞

2015/09/14　日本生物物理学会

Show all ︎Show 5

Papers 85

Structure and Dynamics of the Bacterial Flagellar Motor Complex. International-journal

Shuichi Nakamura, Tohru Minamino

Biomolecules　14　(12)　2024/11/22

DOI： 10.3390/biom14121488 　

More details Close

Many bacteria swim in liquids and move over solid surfaces by rotating flagella. The bacterial flagellum is a supramolecular protein complex that is composed of about 30 different flagellar proteins ranging from a few to tens of thousands. Despite structural and functional diversities of the flagella among motile bacteria, the flagellum commonly consists of a membrane-embedded rotary motor fueled by an ion motive force across the cytoplasmic membrane, a universal joint, and a helical propeller that extends several micrometers beyond the cell surface. The flagellar motor consists of a rotor and several stator units, each of which acts as a transmembrane ion channel complex that converts the ion flux through the channel into the mechanical work required for force generation. The rotor ring complex is equipped with a reversible gear that is regulated by chemotactic signal transduction pathways. As a result, bacteria can move to more desirable locations in response to environmental changes. Recent high-resolution structural analyses of flagella using cryo-electron microscopy have provided deep insights into the assembly, rotation, and directional switching mechanisms of the flagellar motor complex. In this review article, we describe the current understanding of the structure and dynamics of the bacterial flagellum.
It’s not all about flagella – sticky invasion by pathogenic spirochetes Peer-reviewed

Martin Strnad, Nobuo Koizumi, Shuichi Nakamura, Marie Vancová, Ryan O.M. Rego

Trends in Parasitology　40　(5)　378-385　2024/05
Publisher: Elsevier BV
DOI： 10.1016/j.pt.2024.03.004 　

ISSN： 1471-4922
The Role of Morphological Adaptability inVibrio cholerae’s Motility and Pathogenicity

Jun Xu, Keigo Abe, Toshio Kodama, Marzia Sultana, Denise Chac, Susan M. Markiewicz, Erika Kuba, Shiyu Tsunoda, Munirul Alam, Ana A. Weil, Shuichi Nakamura, Tetsu Yamashiro

2024/03/28
Publisher: Cold Spring Harbor Laboratory
DOI： 10.1101/2024.03.27.586043 　

More details Close

ABSTRACT Vibrio cholerae, the etiological agent of cholera, exhibits remarkable adaptability to different environmental conditions by undergoing morphological changes that significantly contribute to its pathogenicity and impact the epidemiology of the disease globally. This study investigates the morphological adaptability of the clinically isolatedV. choleraeO1 strain, specifically focusing on the motility and pathogenicity differences between the filamentous and original comma-shaped forms within diverse viscosity conditions. Utilizing the El Tor strain ofV. choleraeO1, we induced the transformation into the filamentous form and performed a comparative analysis with the canonical comma-shaped morphology. Our approach involved assessing motility patterns, swimming speeds, rotation rates, kinematics, and reversal frequencies through dark-field microscopy and high-speed imaging techniques. The findings reveal that filamentousV. choleraecell retains enhanced motility in viscous environments. This suggests an evolutionary adaptation enabling survival across a range of habitats, notably the human gastrointestinal tract. Filamentous forms demonstrated increased reversal behavior at mucin interfaces, hinting at an advantage in penetrating the mucus layer. Rabbit intestinal loop assays further showed that both morphological forms exhibit similar fluid accumulation ratios, thus indicating comparable pathogenic potentials. These results underscore the significance ofV. cholerae’s morphological flexibility in adapting to environmental viscosity changes, shedding light on the bacterium’s intricate survival and infection strategies. Our study provides critical insights into the dynamics of cholera, underlining the importance of considering bacterial morphology in developing effective cholera control strategies.
Collective gradient sensing by dilute swimming bacteria without clustering Peer-reviewed

Tatsuro Kai, Takahiro Abe, Natsuhiko Yoshinaga, Shuichi Nakamura, Seishi Kudo, Shoichi Toyabe

Phys. Rev. Research　2024
Machine learning-based motion tracking reveals an inverse correlation between adhesivity and surface motility of the leptospirosis spirochete Peer-reviewed

Keigo Abe, Nobuo Koizumi, Shuichi Nakamura

Nature Communications　14　(1)　2023/12/05
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41467-023-43366-0 　

eISSN： 2041-1723

More details Close

Abstract Bacterial motility is often a crucial virulence factor for pathogenic species. A common approach to study bacterial motility is fluorescent labeling, which allows detection of individual bacterial cells in a population or in host tissues. However, the use of fluorescent labeling can be hampered by protein expression stability and/or interference with bacterial physiology. Here, we apply machine learning to microscopic image analysis for label-free motion tracking of the zoonotic bacterium Leptospira interrogans on cultured animal cells. We use various leptospiral strains isolated from a human patient or animals, as well as mutant strains. Strains associated with severe disease, and mutant strains lacking outer membrane proteins (OMPs), tend to display fast mobility and reduced adherence on cultured kidney cells. Our method does not require fluorescent labeling or genetic manipulation, and thus could be applied to study motility of many other bacterial species.
Analysis of Adhesion and Surface Motility of a Spirochete Bacterium

Shuichi Nakamura, Jun Xu, Nobuo Koizumi

Methods in Molecular Biology　159-168　2023/02/27
Publisher: Springer US
DOI： 10.1007/978-1-0716-3060-0_14 　

ISSN： 1064-3745

eISSN： 1940-6029
Frontiers of microbial movement research

Tohru Minamino, Daisuke Nakane, Shuichi Nakamura, Hana Kiyama, Yusuke V. Morimoto, Makoto Miyata

Biophysics and Physicobiology　2023
Publisher: Biophysical Society of Japan
DOI： 10.2142/biophysico.bppb-v20.0033 　

eISSN： 2189-4779
Force Measurement of Bacterial Swimming Using Optical Tweezers. International-journal

Keigo Abe, Kyosuke Takabe, Shuichi Nakamura

Methods in molecular biology (Clifton, N.J.)　2646　169-179　2023

DOI： 10.1007/978-1-0716-3060-0_15 　

More details Close

Velocity is a physical parameter most commonly used to quantify bacterial swimming. In the steady-state motion at a low Reynolds number, the swimming force can be estimated from the swimming velocity and the drag coefficient based on the assumption that the swimming force balances with the drag force exerted on the bacterium. Though the velocity-force relation provides a significant clue to understand the swimming mechanism, the odd configuration of bacteria could develop problems with the accuracy of the force estimation. This chapter describes the force measurement using optical tweezers. The method uses parameters obtained from the shape and movement of a microsphere attached to the bacteria, improving the quantitativeness of force measurement.
High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Having a Rod-Like Straight Hook. International-journal

Shuichi Nakamura, Tohru Minamino

Methods in molecular biology (Clifton, N.J.)　2646　125-131　2023

DOI： 10.1007/978-1-0716-3060-0_11 　

More details Close

The bacterial flagellar motor is embedded within the cell envelop and rotates the long helical filament, which acts as a molecular screw to propel the bacterium. The flagellar motor comprises a rotor and a dozen stator units, converting ion flux through the stator unit into torque. However, the energy coupling mechanism has not been fully understood. Various methods for rotation measurement have been developed to understand the rotation mechanism of the flagellar motor, but the most preferred method in recent studies is a bead assay, which tracks the rotation of a micron to submicron bead attached to the partially sheared flagellar filament at high temporal and spatial resolutions. The bead assay allows us to assess the motor rotation over a wide range of external load, but the elasticity of the axial parts of the flagellum, such as the hook and filament, limits the spatiotemporal resolution. In this chapter, we describe a bead assay optimized for the analysis of the flagellar motor dynamics at near zero load.
Light dependent synthesis of a nucleotide second messenger controls the motility of a spirochete bacterium Peer-reviewed

Jun Xu, Nobuo Koizumi, Yusuke V. Morimoto, Ryo Ozuru, Toshiyuki Masuzawa, Shuichi Nakamura

Scientific Reports　12　(1)　2022/12
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41598-022-10556-7 　

eISSN： 2045-2322

More details Close

Abstract Nucleotide second messengers are universally crucial factors for the signal transduction of various organisms. In prokaryotes, cyclic nucleotide messengers are involved in the bacterial life cycle and in functions such as virulence and biofilm formation, mainly via gene regulation. Here, we show that the swimming motility of the soil bacterium Leptospira kobayashii is rapidly modulated by light stimulation. Analysis of a loss-of-photoresponsivity mutant obtained by transposon random mutagenesis identified the novel sensory gene, and its expression in Escherichia coli through codon optimization elucidated the light-dependent synthesis of cyclic adenosine monophosphate (cAMP). GFP labeling showed the localization of the photoresponsive enzyme at the cell poles where flagellar motors reside. These findings suggest a new role for cAMP in rapidly controlling the flagella-dependent motility of Leptospira and highlight the global distribution of the newly discovered photoactivated cyclase among diverse microbial species.
Complete Genome Sequence of Leptospira kobayashii Strain E30, Isolated from Soil in Japan International-journal Peer-reviewed

Ryo Nakao, Toshiyuki Masuzawa, Shuichi Nakamura, Nobuo Koizumi

Microbiology Resource Announcements　10　(45)　e0090721　2021/11/11
Publisher: American Society for Microbiology
DOI： 10.1128/mra.00907-21 　

eISSN： 2576-098X

More details Close

The spirochete bacterium <named-content content-type="genus-species">Leptospira kobayashii</named-content> is a recently designated species of the genus <italic>Leptospira</italic> . Here, we report the complete genome sequence of <named-content content-type="genus-species">L. kobayashii</named-content> strain E30, consisting of two circular chromosomes and two plasmids.
Crawling motility of Treponema denticola modulated by outer sheath protein Peer-reviewed

Eitoyo Kokubu, Yuichiro Kikuchi, Kazuko Okamoto‐Shibayama, Shuichi Nakamura, Kazuyuki Ishihara

Microbiology and Immunology　2021/09/09
Publisher: Wiley
DOI： 10.1111/1348-0421.12940 　

ISSN： 0385-5600

eISSN： 1348-0421
Corrigendum: Crawling Motility on the Host Tissue Surfaces Is Associated With the Pathogenicity of the Zoonotic Spirochete Leptospira (Frontiers in Microbiology, (2020), 11, (1886), 10.3389/fmicb.2020.01886)

Jun Xu, Nobuo Koizumi, Shuichi Nakamura

Frontiers in Microbiology　12　2021/08/17
Publisher: Frontiers Media S.A.
DOI： 10.3389/fmicb.2021.736406 　

ISSN： 1664-302X
Light-dependent synthesis of a nucleotide second messenger controls bacterial motility

Jun Xu, Nobuo Koizumi, Yusuke V. Morimoto, Ryo Ozuru, Toshiyuki Masuzawa, Shuichi Nakamura

bioRxiv　2021/07/06
Publisher: Cold Spring Harbor Laboratory
DOI： 10.1101/2021.07.06.451194 　

More details Close

Nucleotide second messengers are universally crucial factors for the signal transduction of various organisms. In prokaryotes, cyclic nucleotide messengers are involved in the bacterial life cycle and function, such as virulence, biofilm formation, and others mainly via gene regulation. Here we show that the swimming motility of a soil bacterium is rapidly modulated by cyclic adenosine monophosphate (cAMP) synthesized upon light exposure. Analysis of a loss-of-photoresponsivity mutant obtained by transposon random mutagenesis determined the novel sensory gene, and its expression in <italic>Escherichia coli</italic> through codon optimization revealed the light-dependent synthesis of cAMP. GFP labeling showed the localization of the photoresponsive enzyme at the cell poles where flagellar motors reside. The present findings highlight the new role of cAMP that rapidly controls the flagella-dependent bacterial motility and the global distribution of the discovered photoactivated cyclase among diverse microbial species.
Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cells Peer-reviewed

Isabel Sebastián, Nobuhiko Okura, Bruno M. Humbel, Jun Xu, Idam Hermawan, Chiaki Matsuura, Malgorzata Hall, Chitoshi Takayama, Tetsu Yamashiro, Shuichi Nakamura, Claudia Toma

Cellular Microbiology　23　(9)　2021/05/06
Publisher: Wiley
DOI： 10.1111/cmi.13343 　

ISSN： 1462-5814

eISSN： 1462-5822
Cooperative stator assembly of bacterial flagellar motor mediated by rotation International-journal Peer-reviewed

Kenta I. Ito, Shuichi Nakamura, Shoichi Toyabe

Nature Communications　12　(1)　3218-3218　2021/05
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41467-021-23516-y 　

eISSN： 2041-1723

More details Close

<title>Abstract</title>Cooperativity has a central place in biological regulation, providing robust and highly-sensitive regulation. The bacterial flagellar motor implements autonomous torque regulation based on the stator’s dynamic structure; the stator units bind to and dissociate from the motor dynamically in response to environmental changes. However, the mechanism of this dynamic assembly is not fully understood. Here, we demonstrate the cooperativity in the stator assembly dynamics. The binding is slow at the stalled state, but externally forced rotation as well as driving by motor torque in either direction boosts the stator binding. Hence, once a stator unit binds, it drives the rotor and triggers the avalanche of succeeding bindings. This cooperative mechanism based on nonequilibrium allostery accords with the recently-proposed gear-type coupling between the rotor and stator.
Implications of back-and-forth motion and powerful propulsion for spirochetal invasion International-journal Peer-reviewed

Keigo Abe, Toshiki Kuribayashi, Kyosuke Takabe, Shuichi Nakamura

Scientific Reports　10　(1)　13937-13937　2020/12
Publisher: Springer Science and Business Media LLC
DOI： 10.1038/s41598-020-70897-z 　

eISSN： 2045-2322

More details Close

The spirochete Leptospira spp. can move in liquid and on a solid surface using two periplasmic flagella (PFs), and its motility is an essential virulence factor for the pathogenic species. Mammals are infected with the spirochete through the wounded dermis, which implies the importance of behaviors on the boundary with such viscoelastic milieu; however, the leptospiral pathogenicity involving motility remains unclear. We used a glass chamber containing a gel area adjoining the leptospiral suspension to resemble host dermis exposed to contaminated water and analyzed the motility of individual cells at the liquid-gel border. Insertion of one end of the cell body to the gel increased switching of the swimming direction. Moreover, the swimming force of Leptospira was also measured by trapping single cells using an optical tweezer. It was found that they can generate [Formula: see text] 17 pN of force, which is [Formula: see text] 30 times of the swimming force of Escherichia coli. The force-speed relationship suggested the load-dependent force enhancement and showed that the power (the work per unit time) for the propulsion is [Formula: see text] 3.1 × 10-16 W, which is two-order of magnitudes larger than the propulsive power of E. coli. The powerful and efficient propulsion of Leptospira using back-and-forth movements could facilitate their invasion.
Crawling Motility on the Host Tissue Surfaces Is Associated With the Pathogenicity of the Zoonotic Spirochete Leptospira International-journal Peer-reviewed

Jun Xu, Nobuo Koizumi, Shuichi Nakamura

Frontiers in Microbiology　11　1886-1886　2020/08/05
Publisher: Frontiers Media SA
DOI： 10.3389/fmicb.2020.01886 　

eISSN： 1664-302X

More details Close

Bacterial motility is crucial for many pathogenic species in the process of invasion and/or dissemination. The spirochete bacteria Leptospira spp. cause symptoms, such as hemorrhage, jaundice, and nephritis, in diverse mammals including humans. Although loss-of-motility attenuate the spirochete's virulence, the mechanism of the motility-dependent pathogenicity is unknown. Here, focusing on that Leptospira spp. swim in liquid and crawl on solid surfaces, we investigated the spirochetal dynamics on the host tissues by infecting cultured kidney cells from various species with pathogenic and non-pathogenic leptospires. We found that, in the case of the pathogenic leptospires, a larger fraction of bacteria attached to the host cells and persistently traveled long distances using the crawling mechanism. Our results associate the kinetics and kinematic features of the spirochetal pathogens with their virulence.
Cooperative stator assembly of bacterial flagellar motor for autonomous torque regulation

Kenta I. Ito, Shuichi Nakamura, Shoichi Toyabe

2020/04/27
Publisher: Cold Spring Harbor Laboratory
DOI： 10.1101/2020.04.26.059089 　

More details Close

<title>ABSTRACT</title>Cooperativity has a central place in biological regulation, providing robust and highly-sensitive regulation. The bacterial flagellar motor (BFM) implements autonomous torque regulation by the nonequilibrium structure of the stators; the stators assemble at high load and disperse at low load. It would be natural to suppose that the stator packing is affected by stator-stator interaction. However, the cooperativity among the stators has rarely been explored. Here, we evaluated the energetics and kinetics of the stator assembly by combining dynamic load control of a single motor and the trajectory analysis based on statistical mechanics. We demonstrate that the BFM exploits the dynamic cooperativity of the stator binding for the autonomous torque regulation. The cooperative assembly leads to a discontinuous phase transition and hysteresis, which may implement torque regulation with high sensitivity and robustness.
The 57th Annual Meeting of the Biophysical Society of Japan Peer-reviewed

Shuichi Nakamura, Azusa Kage

Biophysical Reviews　12　(2)　293-294　2020/04
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s12551-020-00629-0 　

ISSN： 1867-2450

eISSN： 1867-2469
Direct observation of speed fluctuations of flagellar motor rotation at extremely low load close to zero International-journal Peer-reviewed

Shuichi Nakamura, Yuta Hanaizumi, Yusuke V. Morimoto, Yumi Inoue, Marc Erhardt, Tohru Minamino, Keiichi Namba

Molecular Microbiology　113　(4)　755-765　2020/04
Publisher: Wiley
DOI： 10.1111/mmi.14440 　

ISSN： 0950-382X

eISSN： 1365-2958

More details Close

The bacterial flagellar motor accommodates ten stator units around the rotor to produce large torque at high load. But when external load is low, some previous studies showed that a single stator unit can spin the rotor at the maximum speed, suggesting that the maximum speed does not depend on the number of active stator units, whereas others reported that the speed is also dependent on the stator number. To clarify these two controversial observations, much more precise measurements of motor rotation would be required at external load as close to zero as possible. Here, we constructed a Salmonella filament-less mutant that produces a rigid, straight, twice longer hook to efficiently label a 60 nm gold particle and analyzed flagellar motor dynamics at low load close to zero. The maximum motor speed was about 400 Hz. Large speed fluctuations and long pausing events were frequently observed, and they were suppressed by either over-expression of the MotAB stator complex or increase in the external load, suggesting that the number of active stator units in the motor largely fluctuates near zero load. We conclude that the lifetime of the active stator unit becomes much shorter when the motor operates near zero load.
Tree of motility – A proposed history of motility systems in the tree of life Peer-reviewed

Makoto Miyata, Robert C. Robinson, Taro Q. P. Uyeda, Yoshihiro Fukumori, Shun‐ichi Fukushima, Shin Haruta, Michio Homma, Kazuo Inaba, Masahiro Ito, Chikara Kaito, Kentaro Kato, Tsuyoshi Kenri, Yoshiaki Kinosita, Seiji Kojima, Tohru Minamino, Hiroyuki Mori, Shuichi Nakamura, Daisuke Nakane, Koji Nakayama, Masayoshi Nishiyama, Satoshi Shibata, Katsuya Shimabukuro, Masatada Tamakoshi, Azuma Taoka, Yosuke Tashiro, Isil Tulum, Hirofumi Wada, Ken‐ichi Wakabayashi

Genes to Cells　25　(1)　6-21　2020/01
Publisher: Wiley
DOI： 10.1111/gtc.12737 　

ISSN： 1356-9597

eISSN： 1365-2443
Evaluation of the Duty Ratio of the Bacterial Flagellar Motor by Dynamic Load Control. International-journal Peer-reviewed

Kento Sato, Shuichi Nakamura, Seishi Kudo, Shoichi Toyabe

Biophysical journal　116　(10)　1952-1959　2019/05/21

DOI： 10.1016/j.bpj.2019.04.004 　

More details Close

The bacterial flagellar motor is one of the most complex and sophisticated nanomachineries in nature. A duty ratio D is a fraction of time that the stator and the rotor interact and is a fundamental property to characterize the motor but remains to be determined. It is known that the stator units of the motor bind to and dissociate from the motor dynamically to control the motor torque depending on the load on the motor. At low load, at which the kinetics such as proton translocation speed limits the rotation rate, the dependency of the rotation rate on the number of stator units N implies D: the dependency becomes larger for smaller D. Contradicting observations supporting both the small and large D have been reported. A dilemma is that it is difficult to explore a broad range of N at low load because the stator units easily dissociate, and N is limited to one or two at vanishing load. Here, we develop an electrorotation method to dynamically control the load on the flagellar motor of Salmonella with a calibrated magnitude of the torque. By instantly reducing the load for keeping N high, we observed that the speed at low load depends on N, implying a small duty ratio. We recovered the torque-speed curves of individual motors and evaluated the duty ratio to be 0.14 ± 0.04 from the correlation between the torque at high load and the rotation rate at low load.
Comparison of Leptospira species isolated from environmental water and soil in Japan Peer-reviewed

Masuzawa T, Uno R, Matsuhashi N, Yamaguchi M, Xu J, Nakamura S

Microbiol. Immunol.　63　(11)　469-473　2019
Publisher: Wiley
DOI： 10.1111/1348-0421.12741 　

ISSN： 0385-5600

eISSN： 1348-0421
Effects of fermentation products of the commensal bacterium Clostridium ramosum on motility, intracellular pH, and flagellar synthesis of enterohemorrhagic Escherichia coli Peer-reviewed

Xu J, Koyanagi Y, Isogai E, Nakamura S

Arch. Microbiol.　201　(6)　841-846　2019
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s00203-019-01656-6 　

ISSN： 0302-8933

eISSN： 1432-072X
Effect of the MotA(M206I) mutation on torque generation and stator assembly in the Salmonella H+-driven flagellar motor Peer-reviewed

Suzuki Y, Morimoto YV, Oono K, Hayashi F, Oosawa K, Kudo S, Nakamura S

J. Bacteriol.　201　(6)　e00727-18　2019

DOI： 10.1128/JB.00727-18 　

ISSN： 0021-9193

eISSN： 1098-5530
Salmonella Typhimurium is attracted to egg yolk and repelled by albumen Peer-reviewed

Okuno K, Xu J, Isogai E, Nakamura S

Curr. Microbiol.　76　(4)　393-397　2019
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s00284-018-1619-5 　

ISSN： 0343-8651

eISSN： 1432-0991
Calibrated electrorotation for characterizing the torque generation by individual flagellar motors

Kento Sato, Shuichi Nakamura, Seishi Kudo, Shoichi Toyabe

arXiv　2018/06
The mechanism of two-phase motility in the spirochete Leptospira: Swimming and crawling Peer-reviewed

Tahara H, Takabe K, Sasaki Y, Kasuga K, Kawamoto A, Koizumi N, Nakamura S

Sci. Adv.　4　eaar7975　2018
Leptospiral flagellar sheath protein FcpA interacts with FlaA2 and FlaB1 in Leptospira biflexa. Peer-reviewed

Sasaki Y, Kawamoto A, Tahara H, Kasuga K, Sato R, Ohnishi M, Nakamura S, Koizumi N

PLOS One　13　e0194923　2018
Implications of coordinated cell-body rotations for Leptospira motility Peer-reviewed

Kyosuke Takabe, Akihiro Kawamoto, Hajime Tahara, Seishi Kudo, Shuichi Nakamura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　491　(4)　1040-1046　2017/09

DOI： 10.1016/j.bbrc.2017.08.007 　

ISSN： 0006-291X

eISSN： 1090-2104
Load- and polysaccharide-dependent activation of the Na+-type MotPS stator in the Bacillus subtilis flagellar motor Peer-reviewed

Naoya Terahara, Yukina Noguchi, Shuichi Nakamura, Nobunori Kami-Ike, Masahiro Ito, Keiichi Namba, Tohru Minamino

SCIENTIFIC REPORTS　7　46081　2017/04

DOI： 10.1038/srep46081 　

ISSN： 2045-2322
Viscosity-dependent variations in the cell shape and swimming manner of Leptospira Peer-reviewed

Kyosuke Takabe, Hajime Tahara, Md. Shafiqul Islam, Samia Affroze, Seishi Kudo, Shuichi Nakamura

MICROBIOLOGY-SGM　163　(2)　153-160　2017/02

DOI： 10.1099/mic.0.000420 　

ISSN： 1350-0872

eISSN： 1465-2080
Bioconvection induced by bacterial chemotaxis in a capillary assay Peer-reviewed

Takahiro Abe, Shuichi Nakamura, Seishi Kudo

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　483　(1)　277-282　2017/01

DOI： 10.1016/j.bbrc.2016.12.152 　

ISSN： 0006-291X

eISSN： 1090-2104
Characterization of Leptospiral Chemoreceptors Using a Microscopic Agar Drop Assay Peer-reviewed

Samia Affroze, Md. Shafiqul Islam, Kyosuke Takabe, Seishi Kudo, Shuichi Nakamura

CURRENT MICROBIOLOGY　73　(2)　202-205　2016/08

DOI： 10.1007/s00284-016-1049-1 　

ISSN： 0343-8651

eISSN： 1432-0991
Mannose-binding lectin inhibits the motility of pathogenic Salmonella through effect to membrane potential and chemotactic pattern Peer-reviewed

Xu J, Nakamura S, Md. I Shafiqul, Guo Y, Ihara K, Tomioka R, Masuda M, Yoneyama H, Isogai E

PLoS One　11　(4)　e0154165-e0154165　2016/04/22
Publisher: Public Library of Science (PLoS)
DOI： 10.1371/journal.pone.0154165 　

eISSN： 1932-6203

More details Close

IF: 3.234
Mannose-Binding Lectin Inhibits the Motility of Pathogenic Salmonella by Affecting the Driving Forces of Motility and the Chemotactic Response Peer-reviewed

Jun Xu, Shuichi Nakamura, Md. Shafiqul Islam, Yijie Guo, Kohei Ihara, Rintaro Tomioka, Mizuki Masuda, Hiroshi Yoneyama, Emiko Isogai

PLOS ONE　11　(4)　e0154165　2016/04

DOI： 10.1371/journal.pone.0154165 　

ISSN： 1932-6203
H+ and Na+ are involved in flagellar rotation of the spirochete Leptospira Peer-reviewed

Md. Shafiqul Islam, Yusuke V. Morimoto, Seishi Kudo, Shuichi Nakamura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　466　(2)　196-200　2015/10

DOI： 10.1016/j.bbrc.2015.09.004 　

ISSN： 0006-291X

eISSN： 1090-2104
Giant Acceleration of Diffusion Observed in a Single-Molecule Experiment on F-1-ATPase Peer-reviewed

Ryunosuke Hayashi, Kazuo Sasaki, Shuichi Nakamura, Seishi Kudo, Yuichi Inoue, Hiroyuki Noji, Kumiko Hayashi

PHYSICAL REVIEW LETTERS　114　(24)　248101　2015/06

DOI： 10.1103/PhysRevLett.114.248101 　

ISSN： 0031-9007

eISSN： 1079-7114
A lactose fermentation product produced by Lactococcus lactis subsp lactis, acetate, inhibits the motility of flagellated pathogenic bacteria Peer-reviewed

Shuichi Nakamura, Yusuke V. Morimoto, Seishi Kudo

MICROBIOLOGY-SGM　161　701-707　2015/04

DOI： 10.1099/mic.0.000031 　

ISSN： 1350-0872
Leptospiral lipopolysaccharide stimulates the expression of toll-like receptor 2 and cytokines in pig fibroblasts Peer-reviewed

Yijie Guo, Tomokazu Fukuda, Kenichiro Donai, Kengo Kuroda, Mizuki Masuda, Shuichi Nakamura, Hiroshi Yoneyama, Emiko Isogai

ANIMAL SCIENCE JOURNAL　86　(2)　238-244　2015/02

DOI： 10.1111/asj.12254 　

ISSN： 1344-3941

eISSN： 1740-0929
Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction Peer-reviewed

Yijie Guo, Tomokazu Fukuda, Shuichi Nakamura, Lanlan Bai, Jun Xu, Kengo Kuroda, Rintaro Tomioka, Hiroshi Yoneyama, Emiko Isogai

ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　28　(2)　273-279　2015/02

DOI： 10.5713/ajas.14.0440 　

ISSN： 1011-2367

eISSN： 1976-5517
Mannose-binding lectin impairs Leptospira activity through the inhibitory effect on the motility of cell Peer-reviewed

Jun Xu, Yijie Guo, Shuichi Nakamura, Md. Shafiqul Islam, Rintaro Tomioka, Hiroshi Yoneyama, Emiko Isogai

MICROBIOLOGICAL RESEARCH　171　21-25　2015

DOI： 10.1016/j.micres.2014.12.010 　

ISSN： 0944-5013
Analysis of the chemotactic behaviour of Leptospira using microscopic agar-drop assay Peer-reviewed

Md. Shafiqul Islam, Kyosuke Takabe, Seishi Kudo, Shuichi Nakamura

FEMS MICROBIOLOGY LETTERS　356　(1)　39-44　2014/07

DOI： 10.1111/1574-6968.12495 　

ISSN： 0378-1097

eISSN： 1574-6968
2SCA-03 Structure of the bacterial flagellar motor involved in the directional switching mechanism(2SCA Japan-China-Taiwan joint sympoium on cooperativity in supramolcular machine,Symposium,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Miyata Tomoko, Kato Takayuki, Morimoto Yusuke V., Nakamura Shuichi, Matsunami Hideyuki, Namba Keiichi

Seibutsu Butsuri　54　(1)　S127　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S127_2 　
1SAP-05 Swimming dynamics and energetics of the spirochete Leptospira(1SAP Supramoleular motility machinery functioning in multi-scale scenes,Symposium,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Nakamura Shuichi

Seibutsu Butsuri　54　(1)　S120　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S120_4 　
3P151 Giant Acceleration of diffusion in F1-ATPase II(Molecular motor,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Ryunosuke Hayashi, Shuichi Nakamura, Seishi Kudo, Kazuo Sasaki, Hiroyuki Noji, Kumiko Hayashi

Seibutsu Butsuri　54　(1)　S274　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S274_1 　
3P199 Ion selectivity of the Leptospira flagellar motor(Cell biology,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Md. Shafiqul Islam, V. Morimoto Yusuke, Seishi Kudo, Shuichi Nakamura

Seibutsu Butsuri　54　(1)　S282　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S282_1 　
2P192 Formation process of a ring-like pattern induced by bacterial chemotaxis(12. Cell biology,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Sato Tadahiko, Nakamura Shuichi, Kudo Seishi

Seibutsu Butsuri　54　(1)　S226　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S226_6 　
2P191 Rotation analysis of the spirochete cell body by 3D dark-field microscopy(12. Cell biology,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Takabe Kyosuke, Islam Md. Shafiqul, Kudo Seishi, Nakamura Shuichi

Seibutsu Butsuri　54　(1)　S226　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S226_5 　
2P171 Motility analysis of Pseudomonas syringae possessing two different stator systems(12. Cell biology,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Tensaka Takuto, Nakamura Shuichi, Kudo Seishi

Seibutsu Butsuri　54　(1)　S223　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S223_3 　
2P190 Chemotactic behavior of Salmonella and bioconvection(12. Cell biology,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Abe Takahiro, Nakamura Shuichi, Kudo Seishi

Seibutsu Butsuri　54　(1)　S226　2014
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.54.S226_4 　
Load-sensitive coupling of proton translocation and torque generation in the bacterial flagellar motor Peer-reviewed

Yong-Suk Che, Shuichi Nakamura, Yusuke V. Morimoto, Nobunori Kami-ike, Keiichi Namba, Tohru Minamino

MOLECULAR MICROBIOLOGY　91　(1)　175-184　2014/01

DOI： 10.1111/mmi.12453 　

ISSN： 0950-382X

eISSN： 1365-2958
Effect of the MotB(D33N) mutation on stator assembly and rotation of the proton-driven bacterial flagellar motor Peer-reviewed

Shuichi Nakamura, Tohru Minamino, Nobunori Kami-Ike, Seishi Kudo, Keiichi Namba

Biophysics (Japan)　10　35-41　2014
Publisher: Biophysical Society of Japan
DOI： 10.2142/biophysics.10.35 　

ISSN： 1349-2942
Direct Measurement of Helical Cell Motion of the Spirochete Leptospira Peer-reviewed

Shuichi Nakamura, Alexander Leshansky, Yukio Magariyama, Keiichi Namba, Seishi Kudo

BIOPHYSICAL JOURNAL　106　(1)　47-54　2014/01

DOI： 10.1016/j.bpj.2013.11.1118 　

ISSN： 0006-3495

eISSN： 1542-0086
Common Evolutionary Origin for the Rotor Domain of Rotary Atpases and Flagellar Protein Export Apparatus Peer-reviewed

Jun-ichi Kishikawa, Tatsuya Ibuki, Shuichi Nakamura, Astuko Nakanishi, Tohru Minamino, Tomoko Miyata, Keiichi Namba, Hiroki Konno, Hiroshi Ueno, Katsumi Imada, Ken Yokoyama

PLOS ONE　8　(5)　e64695　2013/05

DOI： 10.1371/journal.pone.0064695 　

ISSN： 1932-6203
The Inhibition Effect of Antiserum on the Motility of Leptospira Peer-reviewed

Yijie Guo, Shuichi Nakamura, Tasuke Ando, Hiroshi Yoneyama, Seishi Kudo, Emiko Isogai

CURRENT MICROBIOLOGY　66　(4)　359-364　2013/04

DOI： 10.1007/s00284-012-0281-6 　

ISSN： 0343-8651
Effect of osmolarity and viscosity on the motility of pathogenic and saprophytic Leptospira Peer-reviewed

Kyosuke Takabe, Shuichi Nakamura, Masamichi Ashihara, Seishi Kudo

Microbiology and Immunology　57　(3)　236-239　2013/03

DOI： 10.1111/1348-0421.12018 　

ISSN： 0385-5600 1348-0421
Distinct roles of highly conserved charged residues at the MotA-FliG interface in bacterial flagellar motor rotation Peer-reviewed

Yusuke V. Morimoto, Shuichi Nakamura, Koichi D. Hiraoka, Keiichi Namba, Tohru Minamino

Journal of Bacteriology　195　(3)　474-481　2013/02

DOI： 10.1128/JB.01971-12 　

ISSN： 0021-9193 1098-5530
2P159 Torque-speed relationship of the flagellar motor consisting of two distinct stators(11. Molecular motor,Poster)

Terahara Naoya, Noguchi Yukina, Nakamura Shuichi, Kamiike Nobunori, Minamino Tohru, Ito Masahiro, Namba Keiichi

Seibutsu Butsuri　53　(1)　S185　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S185_3 　
1P181 Microscopic observation of chemotactic behaviors of Leptospira(12.Cell biology,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Islam Md. Shafiqul, Takabe Kyosuke, Kudo Seishi, Nakamura Shuichi

Seibutsu Butsuri　53　(1)　S135　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S135_6 　
1P178 Bioconvection of Salmonella induced by chemotaxis and gravity(12.Cell biology,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Abe Takahiro, Nakamura Shuichi, Kudo Seishi

Seibutsu Butsuri　53　(1)　S135　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S135_3 　
1P180 Motility analysis of Leptospira in highly viscous environments(12.Cell biology,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Takabe Kyosuke, Islam Md. Shafiqul, Kudo Seishi, Nakamura Shuichi

Seibutsu Butsuri　53　(1)　S135　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S135_5 　
1P179 Effect of in-frame deletion in the periplasmic region of MotB on the torque-speed relationship of Salmonella flagellar motor(12.Cell biology,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Nakamura Shuichi, Morimoto Yusuke V., Castillo David J., Che Yong-Suk, Kami-ike Nobunori, Kudo Seishi, Minamino Tohru, Namba Keiichi

Seibutsu Butsuri　53　(1)　S135　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S135_4 　
3P285 CheY-P binding to the bacterial flagellar motor affects not only the direction but also the speed of rotation(26. Measurements,Poster)

Hiraoka Koichi D., Nakamura Shuichi, Kami-ike Nobunori, Morimoto Yusuke V.

Seibutsu Butsuri　53　(1)　S259　2013
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.53.S259_2 　
The C-terminal periplasmic domain of MotB is responsible for load-dependent control of the number of stators of the bacterial flagellar motor Peer-reviewed

David J. Castillo, Shuichi Nakamura, Yusuke V. Morimoto, Yong-Suk Che, Nobunori Kami-Ike, Seishi Kudo, Tohru Minamino, Keiichi Namba

Biophysics (Japan)　9　173-181　2013

DOI： 10.2142/biophysics.9.173 　

ISSN： 1349-2942
2PS029 Effect of the D33N mutation in MotB on stator assembly of the bacterial flagellar motor(The 50th Annual Meeting of the Biophysical Society of Japan)

Nakamura Shuichi, Minamino Tohru, Kami-ike Nobunori, Kudo Seishi, Namba Keiichi

Seibutsu Butsuri　52　S115　2012
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.52.S115_1 　
2SE-01 Elucidation of the directional switching mechanism of the bacterial flagellar motor(2SE Harmonized supramolecular machinery for motility and its diversity,Symposium,The 50th Annual Meeting of the Biophysical Society of Japan)

Miyata Tomoko, Kato Takayuki, Fujii Takasi, Nakamura Shuichi, Morimoto Yusuke, Minamino Tohru, Matsunami Hideyuki, Namba Keiichi

Seibutsu Butsuri　52　S12-S13　2012
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.52.S12_6 　
2PS046 Chemotactic response of Salmonella in high cell density(The 50th Annual Meeting of the Biophysical Society of Japan)

Abe Takahiro, Nakamura Shuichi, Kudo Seishi

Seibutsu Butsuri　52　S118　2012
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.52.S118_2 　
Flagellin Redundancy in Caulobacter crescentus and Its Implications for Flagellar Filament Assembly Peer-reviewed

Alexandra Faulds-Pain, Christopher Birchall, Christine Aldridge, Wendy D. Smith, Giulia Grimaldi, Shuichi Nakamura, Tomoko Miyata, Joe Gray, Guanglai Li, Jay X. Tang, Keiichi Namba, Tohru Minamino, Phillip D. Aldridge

JOURNAL OF BACTERIOLOGY　193　(11)　2695-2707　2011/06

DOI： 10.1128/JB.01172-10 　

ISSN： 0021-9193
Structural Insight into the Rotational Switching Mechanism of the Bacterial Flagellar Motor Peer-reviewed

Tohru Minamino, Katsumi Imada, Miki Kinoshita, Shuichi Nakamura, Yusuke V. Morimoto, Keiichi Namba

PLOS BIOLOGY　9　(5)　e1000616　2011/05

DOI： 10.1371/journal.pbio.1000616 　

ISSN： 1544-9173
Charged residues in the cytoplasmic loop of MotA are required for stator assembly into the bacterial flagellar motor Peer-reviewed

Yusuke V. Morimoto, Shuichi Nakamura, Nobunori Kami-ike, Keiichi Namba, Tohru Minamino

MOLECULAR MICROBIOLOGY　78　(5)　1117-1129　2010/12

DOI： 10.1111/j.1365-2958.2010.07391.x 　

ISSN： 0950-382X
Evidence for symmetry in the elementary process of bidirectional torque generation by the bacterial flagellar motor Peer-reviewed

Shuichi Nakamura, Nobunori Kami-ike, Jun-ichi P. Yokota, Tohru Minamino, Keiichi Namba

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　107　(41)　17616-17620　2010/10

DOI： 10.1073/pnas.1007448107 　

ISSN： 0027-8424
2P214 Rotation assay of the proton-driven bacterial flagellar motor with a gold nanoparticle(The 48th Annual Meeting of the Biophysical Society of Japan)

Nakamura Shuichi, Kami-ike Nobunori, Sowa Yoshiyuki, Minamino Tohru, Namba Keiichi

Seibutsu Butsuri　50　(2)　S120　2010
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.50.S120_2 　
2P167 The stator complex of the bacterial flagellar motor senses drag force during motor rotation(The 48th Annual Meeting of the Biophysical Society of Japan)

Che Yong-Suk, Minamino Tohru, Nakamura Shuichi, Kami-ike Nobunori, Namba Keiichi

Seibutsu Butsuri　50　(2)　S111-S112　2010
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.50.S111_6 　
1P161 Localization mechanism of the MotA/B complex to be the stator of the proton-driven bacterial flagellar motor(Molecular motor,The 48th Annual Meeting of the Biophysical Society of Japan)

Morimoto Yusuke, Nakamura Shuichi, Kami-ike Nobunori, Namba Keiichi, Minamino Tohru

Seibutsu Butsuri　50　(2)　S47　2010
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.50.S47_5 　
Role of a Conserved Prolyl Residue (Pro173) of MotA in the Mechanochemical Reaction Cycle of the Proton-Driven Flagellar Motor of Salmonella Peer-reviewed

Shuichi Nakamura, Yusuke V. Morimoto, Nobunori Kam-ike, Tohru Minamino, Keiichi Namba

JOURNAL OF MOLECULAR BIOLOGY　393　(2)　300-307　2009/10

DOI： 10.1016/j.jmb.2009.08.022 　

ISSN： 0022-2836
Effect of Intracellular pH on the Torque-Speed Relationship of Bacterial Proton-Driven Flagellar Motor Peer-reviewed

Shuichi Nakamura, Nobunori Kami-ike, Jun-ichi P. Yokota, Seishi Kudo, Tohru Minamino, Keiichi Namba

JOURNAL OF MOLECULAR BIOLOGY　386　(2)　332-338　2009/02

DOI： 10.1016/j.jmb.2008.12.034 　

ISSN： 0022-2836
2P-148 Role of a Conserved Proline Residue, Pro-173, of MotA in the Mechanochemical Reaction Cycle of Proton-Driven Bacterial Flagellar Motor(Cell biology,The 47th Annual Meeting of the Biophysical Society of Japan)

Nakamura Shuichi, Morimoto Yusuke, Kami-ike Nobunori, Minamino Tohru, Namba Keiichi

Seibutsu Butsuri　49　S129　2009
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.49.S129_4 　
Suppressor analysis of the MotB(D33E) mutation to probe bacterial flagellar motor dynamics coupled with proton translocation Peer-reviewed

Yong-Suk Che, Shuichi Nakamura, Seiji Kojima, Nobunori Kami-ike, Keiichi Namba, Tohru Minamino

JOURNAL OF BACTERIOLOGY　190　(20)　6660-6667　2008/10

DOI： 10.1128/JB.00503-08 　

ISSN： 0021-9193

eISSN： 1098-5530
1P-152 Suppressor analysis of the MotB(D33E) mutation to probe the bacterial flagellar motor dynamics coupled with proton translocation(The 46th Annual Meeting of the Biophysical Society of Japan)

Che Yong-Suk, Nakamura Shuichi, Morimoto Yusuke, Kami-ike Nobunori, Namba Keiichi, Minamino Tohru

Seibutsu Butsuri　48　S44-S45　2008
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.48.S44_8 　
1P-182 Effect of Intracellular pH on the H^+ -driven Flagellar Motor of Salmonella(The 46th Annual Meeting of the Biophysical Society of Japan)

Nakamura Shuichi, Minamino Tohru, Kami-ike Nobunori, Kudo Seishi, Namba Keiichi

Seibutsu Butsuri　48　S49　2008
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.48.S49_4 　
2P178 Effect of Intracellular pH on Flagellar Motor Rotation of Salmonella Slow Motile Mutants(Cell biological problems-adhesion, motility, cytoskeleton, signaling,and membrane,Poster Presentations)

Nakamura Shuichi, Minamino Tohru, Kamiike Nobunori, Namba Keiichi

Seibutsu Butsuri　47　S157　2007
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.47.S157_3 　
3P181 Observation of stepping motions of the proton-driven flagellar motor of Salmonella(Molecular motors,Poster Presentations)

Nakamura Shuichi, Minamino Tohru, Kami-ike Nobunori, Namba Keiichi

Seibutsu Butsuri　47　S248　2007
Publisher: The Biophysical Society of Japan General Incorporated Association
DOI： 10.2142/biophys.47.S248_2 　
Improvement in motion eficiency of the spirochete Brachyspira pilosicoli in viscous environments Peer-reviewed

S Nakamura, Y Adachi, T Goto, Y Magariyama

BIOPHYSICAL JOURNAL　90　(8)　3019-3026　2006/04

DOI： 10.1529/biophysj.105.074336 　

ISSN： 0006-3495

Show all ︎Show first 5

Misc. 12

細菌の接着性と運動性の意義～機械学習で明らかになった病原性との関係～ Invited Peer-reviewed

中村修一

生物物理　64　(4)　190-192　2024
Motility of the Zoonotic Spirochete Leptospira: Insight into Association with Pathogenicity Invited Peer-reviewed

Shuichi Nakamura

International Journal of Molecular Sciences　23　(3)　1859-1859　2022/02/07
Publisher: MDPI AG
DOI： 10.3390/ijms23031859 　

eISSN： 1422-0067

More details Close

If a bacterium has motility, it will use the ability to survive and thrive. For many pathogenic species, their motilities are a crucial virulence factor. The form of motility varies among the species. Some use flagella for swimming in liquid, and others use the cell-surface machinery to move over solid surfaces. Spirochetes are distinguished from other bacterial species by their helical or flat wave morphology and periplasmic flagella (PFs). It is believed that the rotation of PFs beneath the outer membrane causes transformation or rolling of the cell body, propelling the spirochetes. Interestingly, some spirochetal species exhibit motility both in liquid and over surfaces, but it is not fully unveiled how the spirochete pathogenicity involves such amphibious motility. This review focuses on the causative agent of zoonosis leptospirosis and discusses the significance of their motility in liquid and on surfaces, called crawling, as a virulence factor.
光活性型アデニル酸シクラーゼによるレプトスピラ・コバヤシイの運動活性化

許駿, 小泉信夫, 森本雄祐, 尾鶴亮, 増澤俊幸, 中村修一

レプトスピラ・シンポジウム抄録(CD-ROM)　58th　2022
Spirochete Flagella and Motility Invited Peer-reviewed

Shuichi Nakamura

Biomolecules　10　(4)　550-550　2020/04/04
Publisher: MDPI AG
DOI： 10.3390/biom10040550 　

eISSN： 2218-273X

More details Close

Spirochetes can be distinguished from other flagellated bacteria by their long, thin, spiral (or wavy) cell bodies and endoflagella that reside within the periplasmic space, designated as periplasmic flagella (PFs). Some members of the spirochetes are pathogenic, including the causative agents of syphilis, Lyme disease, swine dysentery, and leptospirosis. Furthermore, their unique morphologies have attracted attention of structural biologists; however, the underlying physics of viscoelasticity-dependent spirochetal motility is a longstanding mystery. Elucidating the molecular basis of spirochetal invasion and interaction with hosts, resulting in the appearance of symptoms or the generation of asymptomatic reservoirs, will lead to a deeper understanding of host–pathogen relationships and the development of antimicrobials. Moreover, the mechanism of propulsion in fluids or on surfaces by the rotation of PFs within the narrow periplasmic space could be a designing base for an autonomously driving micro-robot with high efficiency. This review describes diverse morphology and motility observed among the spirochetes and further summarizes the current knowledge on their mechanisms and relations to pathogenicity, mainly from the standpoint of experimental biophysics.
Flagella-Driven Motility of Bacteria Invited Peer-reviewed

Shuichi Nakamura, Tohru Minamino

Biomolecules　9　(7)　279-279　2019/07/14
Publisher: MDPI AG
DOI： 10.3390/biom9070279 　

eISSN： 2218-273X

More details Close

The bacterial flagellum is a helical filamentous organelle responsible for motility. In bacterial species possessing flagella at the cell exterior, the long helical flagellar filament acts as a molecular screw to generate thrust. Meanwhile, the flagella of spirochetes reside within the periplasmic space and not only act as a cytoskeleton to determine the helicity of the cell body, but also rotate or undulate the helical cell body for propulsion. Despite structural diversity of the flagella among bacterial species, flagellated bacteria share a common rotary nanomachine, namely the flagellar motor, which is located at the base of the filament. The flagellar motor is composed of a rotor ring complex and multiple transmembrane stator units and converts the ion flux through an ion channel of each stator unit into the mechanical work required for motor rotation. Intracellular chemotactic signaling pathways regulate the direction of flagella-driven motility in response to changes in the environments, allowing bacteria to migrate towards more desirable environments for their survival. Recent experimental and theoretical studies have been deepening our understanding of the molecular mechanisms of the flagellar motor. In this review article, we describe the current understanding of the structure and dynamics of the bacterial flagellum.
Mechanism of bacterial motility Invited

NAKAMURA Shuichi

72　(2)　157-165　2019
菌体内べん毛を利用する螺旋形細菌の遊泳メカニズム Invited Peer-reviewed

高部響介, 川本晃大, 中村修一

生物物理　58　(4)　191-195　2018
スピロヘータの運動メカニズム Invited Peer-reviewed

中村修一

生物工学会誌　96　195-199　2018
A graduate education program "Ethics of life for young engineers / Medical Ethics" - A trial to motivate next generation researchers by engineering and medical divisions

Seishi KUDO, Shuichi NAKAMURA, Tatsuo YOSHINOBU, Takahiro ARIMA, Atsushi ASAI

Bulletin of the Institute for Excellence in Higher Education, Tohoku University　1　(1)　195-199　2015
Publisher: 東北大学高度教養教育・学生支援機構
ISSN： 2189-5945
Morphology and motility of the spirochetes Invited Peer-reviewed

Shuichi Nakamura

日本細菌学雑誌　69　527-538　2014

DOI： 10.3412/jsb.69.527 　
水素イオンで動く細菌べん毛モーターの構造と機能 Invited

中村修一, 南野徹

化学と生物　49　(1)　22-31　2011/01
Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
DOI： 10.1271/kagakutoseibutsu.49.22 　

ISSN： 0453-073X
1145 Improvement in motion efficiency of spirochete in viscous environments

MAGARIYAMA Yukio, NAKAMURA Shuichi, ADACHI Yoshikazu, GOTO Tomonobu

年次大会講演論文集 : JSME annual meeting　2005　(6)　83-84　2005/09/18
Publisher: 一般社団法人日本機械学会
DOI： 10.1299/jsmemecjo.2005.6.0_83 　

More details Close

The motility of spirochete improves in viscous environments. Such phenomena cannot be observed in the macroscopic world. One of the authors has proposed a hypothesis that predicts increase of the motion efficiency with the viscosity because the polymer molecules form a quasi-rigid network. To examine the hypothesis experimentally, we simultaneously measured the swimming speeds and rotation rates of a spirochete Brachyspira pilosicoli in polymer solutions by two-directional-illuminated dark-field microscopy (2DDM), which is an originally developed method to measure rotation rate of a helical body. This experimental result illustrated increase of the motion efficiency with viscosity. Therefore, we concluded that the improvement of spirochete motility in viscous environments at least partly relates with increase of the motion efficiency caused by a quasi-rigid network of polymer molecules.

Show all ︎Show first 5

Books and Other Publications 5

Bacterial and archaeal motility

Tohru Minamino, Makoto Miyata, Keiichi Namba (Editors)

Humana Press　2023

ISBN: 9781071630594
Leptospira spp.

Shuichi Nakamura

Springer US　2020
獣医微生物学第4版

中村修一

文永堂出版　2018
分子マシンの科学分子の動きとその機能を見る

Shuichi Nakamura

Kagakudojin　2017
Methods in Microbiology 1593 “The Bacterial Flagellum, Methods and Protocols”

Nakamura S, Md. Islam S

Springer　2017

Presentations 5

Adhesins and flagella-dependent bacterial surface motility Invited

Shuichi Nakamura

IUPAB 2024　2024/06/28
Mechanism and biological significance of flagella-dependent bacterial motility Invited

Shuichi Nakamura

Active matter 2024　2024/01/26
Inverse correlation between adhesivity and crawling motility in Leptospira interrogans Invited

Biology of Spirochetes, Gordon Research Conference　2024/01/16
Exploring Bacterial Flagella and Motility with Biophysical Approaches Invited

Shuichi Nakamura

IEEE-NANOMED 2023　2023/12/07
Biophysics of Spirochetes Invited

Shuichi Nakamura

2023/11/15

Research Projects 18

細胞通過性と宿主特異性から解き明かすスピロヘータ感染症の病態機構

中村修一, 大濱侑季, 小泉信夫, 川本晃大

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2024/04/01 - 2027/03/31
In vivo 4D X-ray tomography of condensed microbial suspension in diorama environments

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Transformative Research Areas (A)

Institution: Tohoku University

2021/09/10 - 2026/03/31
細菌べん毛の同期性を制御する化学－物理情報変換・伝達の理解

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Institution: Tohoku University

2022/04 - 2024/03
バイオイメージングで解き明かす人獣共通感染症細菌の宿主依存的病原性発現機構

中村修一, 小泉信夫, 加藤貴之

Offer Organization: 日本学術振興会

System: 科学研究費助成事業基盤研究(B)

Category: 基盤研究(B)

Institution: 東北大学

2021/04 - 2024/03
細菌分子モーターの長距離同期を制御する物理情報の時空間コンビネーション

中村修一, 森本雄祐

Offer Organization: 日本学術振興会

System: 科学研究費助成事業新学術領域研究(研究領域提案型)

Category: 新学術領域研究(研究領域提案型)

Institution: 東北大学

2020/04 - 2022/03

More details Close

本研究で材料としたLeptospira kobayashii（レプトスピラ・コバヤシイ）は，青～緑の光によって運動パターンが変化するという現象は明らかであったものの，本研究開始当初は，光センサーが未同定であった。トランスポゾンを用いたランダム変異挿入法によりゲノムワイドに得た変異体ライブラリーから光応答を欠損した株を見つけることができ，そのトランスポゾン挿入位置解析から，光応答欠損株ではアデニル酸シクラーゼモチーフを含む新規の遺伝子が破壊されていることが分かった。光依存的なcAMP合成の測定を試みたが，本来の宿主であるL. kobayashiiでは測定限界に近い低濃度であった。コドン最適化によって，L. kobayashiiの光応答関連遺伝子を大腸菌で過剰発現することに成功し，大腸菌発現系によって光依存的cAMP合成が確認できた。我々は，この遺伝子をLprA（leptospiral photoresponsive protein A）と名付けた。LprAに緑色蛍光蛋白質を標識して細胞内局在を調べたところ，菌体当たりの発現量は多くないが，興味深いことにべん毛モーターが存在する菌体両末端に局在していることがわかった。LprAの極局在は，cAMP濃度をべん毛モーター近傍で局所的に高める信号増幅の効果を示唆する。LprA欠損株は光依存的なcAMP合成能を失うが，膜透過性cAMPの培地への添加に瞬時に応答して野生型と同様の運動変化を示すことも明らかとなった。この結果は，cAMPがべん毛運動に作用していることを示す。光活性型アデニル酸シクラーゼはミドリムシで初めて発見され，いくつかの土壌細菌でも類似遺伝子が見つかっている。しかし，細菌の運動を光刺激後1秒程度で瞬時に変化させる例はない。新しいシグナル経路の発見ならびにオプトジェネティクスの新材料開発につながることが期待できる。
Predatory bacteria as a safe, viable antibiotic against pathogenic microbes: combatting antimicrobial resistance

Offer Organization: The Daiwa Anglo-Japanese Foundation

System: Daiwa Foundation Award

2020/01 - 2021/06
レプトスピラ症病原体の宿主選好メカニズムに関する研究 Competitive

中村修一

Offer Organization: 日本学術振興会

System: 基盤研究（C）代表

2018/04 - 2020/03
腎近位尿細管上皮細胞を足場とするレプトスピラの感染機構の解明 Competitive

Toma Claudia

Offer Organization: 日本学術振興会

System: 基盤研究（B）分担

2019/04 -
トランスポゾン挿入変異体を用いたレプトスピラの宿主持続感染機構の総合的な解析 Competitive

小泉信夫

Offer Organization: 日本学術振興会

System: 基盤研究（C）分担

2019/04 -
レプトスピラ症早期診断法の開発－高速・高感度・簡便・高精度を可能にする新技術－ Competitive

林史夫

Offer Organization: 日本学術振興会

System: 基盤研究（C）分担

2017/04 - 2019/03
キメラ技術で挑戦するスピロヘータべん毛特異的イオンチャネルの機能解析 Competitive

中村修一

Offer Organization: 日本学術振興会

System: 挑戦的萌芽研究

2016/04 - 2018/03
スピロヘータ運動の変形と力学 Competitive

中村修一

System: 新学術領域研究（研究領域提案型）

2015/04 - 2017/03
スピロヘータの推進力発生メカニズム Competitive

中村修一

System: 新学術領域研究（研究領域提案型）

2013/04 - 2015/03
プロトン駆動型べん毛モーターのメカノケミカルサイクルの分子機構 Competitive

中村修一

Offer Organization: 日本学術振興会

System: 若手研究（B）代表

2012/04 - 2015/03
べん毛モータの機械的強度と出力特性 Competitive

工藤成史

Offer Organization: 日本学術振興会

System: 基盤研究（C）分担

2012/04 - 2015/03
ナノバイオマーカーを利用したモデル腸内フローラのリアルタイム機能解析 Competitive

中村修一

Offer Organization: （財）発酵研究所

System: 平成24年度一般研究助成

2012/04 - 2013
離れて存在する分子モーターを同調制御する細胞内情報伝達・分岐の分子機構 Competitive

中村修一

Offer Organization: （財）光科学技術研究振興財団

System: 平成24年度研究助成

2012 - 2013
ナノバイオマーカーを利用したモデル腸内フローラのリアルタイム機能解析 Competitive

中村修一

Offer Organization: 財団法人発酵研究所

System: 平成24年度一般研究助成

2012/04 -

Show all Show first 5

Media Coverage 10

動物細胞上の病原体の動きを機械学習で自動追跡＝東北大など Myself

MIT Technology Review

2023/12/12

Type: Newspaper, magazine
東北大、国立感染症研究所と共同で腎臓細胞に付着した細菌の動きを機械学習によって自動追跡する手法を開発

日本経済新聞（電子版）

2023/12/05

Type: Newspaper, magazine
東北大・琉球大・九州工大など、細菌の泳ぎを一瞬で加速する光応答性蛋白質を発見

日本経済新聞（電子版）

2022/04

Type: Newspaper, magazine
レプトスピラ症，感染の仕組み解明

琉球新報

2021/04/24

Type: Newspaper, magazine
琉球大・沖縄科学技術大・東北大、病原細菌が臓器を壊して感染する仕組みを解明

日本経済新聞（電子版）

2021/04/23

Type: Newspaper, magazine
東北大、レプトスピラ細菌の運動の仕組みを解明

日本経済新聞（電子版）

2018/05/31

Type: Newspaper, magazine
サルモネラ菌スイッチひとつ逆回転

読売新聞

2011/06/27
べん毛モーターの方向転換原子レベルで解明阪大

日刊工業新聞

2011/05/12

Type: Newspaper, magazine
細菌移動、分子の仕組み解明阪大

日経産業新聞

2011/05/12

Type: Newspaper, magazine
細菌べん毛運動両方向同じ動き阪大が仕組み解明

日経産業新聞

2010/09/30

Type: Newspaper, magazine

Show all Show first 5