Details of the Researcher

PHOTO

Jumpei Washio
Section
Graduate School of Dentistry
Job title
Associate Professor
Degree

  • 博士 (Tohoku University)

Research History 5

  • 2023/02 - Present
    東北大学大学院歯学研究科　准教授

  • 2016/10 - 2023/01
    東北大学歯学研究科　講師

  • 2007/04 - 2016/09
    東北大学歯学研究科　助教

  • 2006/04 - 2007/03
    東北大学歯学研究科　助手（研究助手）

  • 2005/04 - 2006/03
    東北大学歯学研究科　研究助手

Education 2

  • Tohoku University　Graduate School, Division of Dental Research　preventive dentistry

    - 2005/03/31

  • Tohoku University　Faculty of Dentistry　歯学科

    - 2001/03/31

Professional Memberships 1

  • 日本細菌学会

    2025/03 - Present

Research Areas 3

  • Life sciences / Oral medicine (pathology) /

  • Life sciences / Oral medicine /

  • Life sciences / Community dentistry /

Awards 2

  1. 東北大学歯学会 学術賞

    2023/10

  2. ＪＡＤＲ学術奨励賞

    2006/08　国際歯科学会日本部会

Papers 94

  1. Microbiota in Green Tea Beverages Remaining in Plastic Bottles After Direct Drinking

    Miho Kawachi, Anna Wakui, Manami Imai, Mirai Sekiguchi, Haruna Sato, Yuki Kato, Rika Okabe, Takashi Abe, Nanase Takahashi, Nagara Kaku, Misato Miyazawa, Aya Sato, Yuka Naruse, Nanami Kubo, Ayaka Misumi, Hiroto Sano, Yuki Abiko, Jumpei Washio, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Microbiology and Immunology　2026/01/20

    Publisher: Wiley

    DOI： 10.1111/1348-0421.70041 　

    ISSN： 0385-5600

    eISSN： 1348-0421

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    ABSTRACT This study compared bacterial counts and composition in plastic‐bottled green tea beverages immediately after direct drinking and after storage at 37°C for 1 day. Immediately after drinking, (1.9 ± 1.6) × 10 3 and (4.5 ± 3.9) × 10 3 colony forming units (CFU)/mL were recovered from standard green tea and strong green tea, respectively. After 1 day of storage, (1.3 ± 1.5) × 10 4 and (1.6 ± 3.1) × 10 3 CFU/mL were recovered from standard green tea and strong green tea, respectively. Following storage, Lacticaseibacillus spp., Streptococcus , and Veillonella were isolated from both beverages.

  2. Microbial profiling of bold green tea bottled beverages: A screening experiment

    Manami Imai, Miho Kawachi, Anna Wakui, Misato Miyazawa, Mirai Sekiguchi, Yuki Kato, Haruna Sato, Yuka Naruse, Nanami Asano, Momoko Morohashi, Hiroto Sano, Yuki Abiko, Jumpei Washio, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of Oral Biosciences　67　(4)　100697-100697　2025/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.job.2025.100697 　

    ISSN： 1349-0079

  3. Microfluidic All-Solid-State Electrodes for Real-Time Monitoring of Salivary Cations (Na+, K+, and Ca2 +). International-journal

    Yoshihisa Ozeki, Tetsuya Yamada, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi, Yasuko Yanagida

    ACS omega　10　(45)　53989-53997　2025/11/18

    DOI： 10.1021/acsomega.5c04887 　

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    Saliva is a valuable biofluid for noninvasive diagnostics, offering insights into oral and systemic health through ion composition analysis. Key electrolytes such as calcium (Ca2 +), sodium (Na+), and potassium (K+) are critical for tooth enamel integrity, remineralization, and caries resistance. Conventional detection methods such as atomic absorption spectroscopy (AAS) and inductively coupled plasma mass spectrometry (ICP-MS) are impractical for real-time or point-of-care applications. Here, we report the fabrication and integration of all-solid-state ion-selective electrodes (ISEs) into a microfluidic flow device for real-time, low-volume, and wireless salivary ion monitoring. The platform enables precise fluid handling, automated processing, and simultaneous multi-ion detection. The fabricated ISEs exhibited near-Nernstian responses for Ca2 + and Na+, while the K+ sensor showed a slightly sub-Nernstian behavior due to membrane deposition effects. Importantly, validation with real human saliva confirmed concentration-dependent responses, stable operation in complex biological matrices, and reproducibility across independent samples. The Na+ sensor demonstrated high selectivity (log10 KPot Na,K = -1.63 and log10 KPot Na,Ca = -4.63), while response times of 3-5 min supported near real-time monitoring. These results demonstrate that integrating solid-state ISEs with microfluidics provides a robust and noninvasive diagnostic platform for oral and systemic health assessment. The system represents a step toward miniaturized, cost-effective, and user-friendly point-of-care tools.

  4. Time-Dependent Changes in Effects of Butyrate on Human Gingival Fibroblasts. International-journal

    Haruki Otani, Jumpei Washio, Aoi Kunitomi, Satoko Sato, Yuki Abiko, Shiori Sasaki, Kazumasa Ohashi, Satoru Yamada, Nobuhiro Takahashi

    Clinical and experimental dental research　11　(3)　e70120　2025/06

    DOI： 10.1002/cre2.70120 　

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    OBJECTIVES: Butyrate is one of major metabolites of periodontitis-associated bacteria and often detected in periodontal pockets. Butyrate has been considered to affect human gingival fibroblasts (HGFs); however, there was no information on its long-term effect as occurs in periodontitis. Therefore, this study aimed to evaluate the time-dependent effects of butyrate on HGFs. MATERIAL AND METHODS: The effects of butyrate on HGF proliferation, apoptosis, cell morphology, glucose metabolic activity, butyrate metabolic activity, and cell migration ability were evaluated by cell counting, DNA electrophoresis, cell staining, pH-stat system, HPLC, and scratch test, respectively. RESULTS: HGF proliferation was temporarily inhibited by 5-10 mM butyrate (p < 0.05); however, it resumed at 24 h with morphological changes from spindle to slightly widened (p < 0.05). HGFs cultured with 10 mM butyrate for 12-24 h shifted the glucose metabolic pathway from oxidative phosphorylation to glycolysis (p < 0.05), and increased butyrate consumption, which returned to control levels over 24 h. HGF migration ability tended to decrease at 72 h. CONCLUSIONS: HGF cell proliferation and glucose/butyrate metabolism were temporarily inhibited by butyrate and then recovered in a time-dependent manner, accompanied by changes in cell morphology. These time-dependent effects may help to understand the role of butyrate in the pathology of periodontitis.

  5. The Effect of Environmental Factors on the Nitrate and Nitrite Metabolism of Oral Actinomyces and Schaalia Species. International-journal

    Tomona Otake, Jumpei Washio, Kazuko Ezoe, Satoko Sato, Yuki Abiko, Kaoru Igarashi, Nobuhiro Takahashi

    Molecular oral microbiology　40　(2)　104-115　2025/04

    DOI： 10.1111/omi.12492 　

    ISSN： 2041-1006

    eISSN： 2041-1014

  6. Microbiome Signatures and Dysbiotic Patterns in Oral Cancer and Precancerous Lesions. International-journal

    Cheng-Chieh Yang, Jumpei Washio, Yu-Cheng Lin, Ming-Lun Hsu, Ding-Han Wang, Fa-Tzu Tsai, Yu-Min Lin, Hsi-Feng Tu, Hsiu-Chuan Chang, Nobuhiro Takahashi

    Oral diseases　2025/03/19

    DOI： 10.1111/odi.15317 　

    ISSN： 1354-523X

    eISSN： 1601-0825

  7. Characterization of Lactococcus lactis subspecies lactis strain Y-PDH01-07 isolated from traditional Indonesian fermented milk (dadih) as an oral probiotic candidate. International-journal

    Ambar Kusuma Astuti, Andi Yasmon, Melva Louisa, Puspita Eka Wuyung, Jumpei Washio, Nobuhiro Takahashi, Yuniardini Septorini Wimardhani

    Science progress　108　(4)　368504251383108-368504251383108　2025

    DOI： 10.1177/00368504251383108 　

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    ObjectiveThe balance of oral microbiota is important in oral disease prevention and treatment. Oral microbiomes play an essential role in oral diseases through anti-inflammatory effects and restoring dysbiosis. Lactococcus lactis subspecies lactis strains Y-PDH01-07 (LL01-07) were identified from dadih, a traditional Indonesian fermented milk. Previous research had shown that dadih can reduce colon inflammation. However, the characteristics of LL01-07 as an oral probiotic have not been explored. The objective of this research is to characterize and functionally assess the potential of LL01-07 as an oral probiotic.MethodsStrains were identified using phenotypic features, PCR, and sequencing, and their biosafety status was evaluated. The oral probiotic characterization of LL01-07 consisted of carbohydrate fermentation ability, antimicrobial activity, auto-aggregation, hydrophobicity, and acid resistance. An in vivo experiment was conducted to evaluate its anti-inflammatory effect on the oral mucosal wound model with unpaired t-test to evaluate the group's significance.ResultsGenotypic results confirmed that all isolates were Lactococcus lactis subsp. lactis, and biosafety assessment showed no inhibition zone in antibiotic resistance test and no hemolytic activity. LL05 had the highest % auto aggregation: 15.0 ± 1.7% at 33 h and 54.2 ± 4.8% at 22 h. LL05 had the best acid resistance test (75.2% survival after 4 h of acid exposure). The lesion severity score did not improve in the treatment group, but body weight increased.ConclusionsLL 01-07 was safe to administer to oral cavity but showed less oral probiotic potential. The in vivo study showed that the treatment group had a lower wound healing effect score but a higher average body weight.

  8. Extracellular Z-DNA Enhances Cariogenicity of Biofilm

    S. L. Han, J. Wang, H. S. Wang, P. Yu, L. Y. Wang, Y. L. Ou, L. J. Ding, J. Washio, N. Takahashi, L. L. Zhang

    Journal of Dental Research　2025

    DOI： 10.1177/00220345251316822 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  9. Candida albicans and NCAC species: acidogenic and fluoride-resistant oral inhabitants. International-journal

    Haneen Raafat Fathi Mousa, Yuki Abiko, Jumpei Washio, Satoko Sato, Nobuhiro Takahashi

    Journal of oral microbiology　17　(1)　2473938-2473938　2025

    DOI： 10.1080/20002297.2025.2473938 　

    eISSN： 2000-2297

  10. Profiling of the microbes on the surface of smartphone touchscreens

    Nanase Takahashi, Anna Wakui, Yume Sekizawa, Miho Kawachi, Mirai Sekiguchi, Takashi Abe, Aya Sato, Misato Miyazawa, Manami Imai, Nagara Kaku, Shingo Maruyama, Hiroto Sano, Nahoko Kakihara, Jumpei Washio, Yuki Abiko, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of Oral Biosciences　100607-100607　2024/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.job.2024.100607 　

    ISSN： 1349-0079

  11. Effects of flushing of dental waterlines in portable dental units on water quality management. International-journal

    Kunihiro Fushimi, Jun Watanabe, Masahiro Yamada, Jumpei Washio, Nobuhiro Takahashi, Hiroshi Egusa

    Journal of dental sciences　19　(Suppl 1)　S61-S69-S69　2024/12

    DOI： 10.1016/j.jds.2024.10.014 　

    ISSN： 1991-7902

    eISSN： 2213-8862

  12. 口腔microbiomeとmicrobiotaの現状と今後の課題 口腔細菌叢からacetaldehyde産生、分解菌を探索する(The Current Reports on Oral Microbiome and Microbiota by Promising Challengers Search for Acetaldehyde-producing and -degrading Bacteria from the Oral Microbiome)

    Sato Chika, Tagaino Ryo, Washio Jumpei, Abiko Yuki, Igarashi Kaoru, Takahashi Nobuhiro

    Journal of Oral Biosciences Supplement　2024　[US8-03]　2024/11

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  13. 口腔Neisseria及び口腔Veillonellaによる口腔マイクロバイオーム内のpH低下に対する抑制効果

    鷲尾 純平, 江副 和子, 佐藤 聡子, 安彦 友希, 高橋 信博

    Journal of Oral Biosciences Supplement　2024　[P3-26]　2024/11

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  14. Antimicrobial effects of epigallocatechin-3-gallate, a catechin abundant in green tea, on periodontal disease-associated bacteria. International-journal

    Mayu Higuchi, Yuki Abiko, Jumpei Washio, Nobuhiro Takahashi

    Archives of oral biology　167　106063-106063　2024/11

    DOI： 10.1016/j.archoralbio.2024.106063 　

    ISSN： 0003-9969

    eISSN： 1879-1506

  15. Molecular microbiological profiling of bottled unsweetened tea beverages: A screening experiment. International-journal

    Nagara Kaku, Miho Kawachi, Anna Wakui, Misato Miyazawa, Manami Imai, Nanase Takahashi, Aya Sato, Takashi Abe, Haruna Sato, Yuki Kato, Rika Okabe, Yuka Naruse, Nao Sato, Nanami Asano, Momoko Morohashi, Hiroto Sano, Jumpei Washio, Yuki Abiko, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of oral biosciences　66　(3)　628-632　2024/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.job.2024.07.006 　

    ISSN： 1349-0079

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    To explore the potential storage and safety of drinking leftover bottled tea beverages from various manufacturers after direct drinking from bottles, we conducted a screening experiment on the growth of salivary bacteria in plastic bottles of tea. The diluted saliva samples from 10 participants were inoculated into the test bottled beverages, which resulted in bacteria, particularly former members of the genus Lactobacillus, growing in some green tea beverages with a neutral pH. In contrast, tea beverages with less bacterial growth contained Streptococcus spp., and the leftovers may be safe to store and drink again.

  16. Effects of Nitrate and Nitrite on Plaque pH Decrease and Nitrite-Producing and -Degrading Activities of Plaque in vitro. International-journal

    Yuji Yamamoto, Jumpei Washio, Koichi Shimizu, Nobuhiro Takahashi

    Caries research　58　(6)　552-561　2024/07/02

    DOI： 10.1159/000540017 　

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    INTRODUCTION: The purpose of this study was to investigate the effects of nitrate and nitrite on the pH-lowering activity of human plaque, the nitrite-producing and -degrading activities of human plaque, and their correlation. METHODS: Nitrate and nitrite were added to human plaque suspensions collected from the buccal aspect of maxillary molars of patients visiting a general dental clinic, and changes in pH were measured with and without glucose addition. Nitrite-producing and -degrading activities were evaluated by adding nitrate and nitrite to the plaque suspension and measuring the increase and decrease in nitrite with Griess reagent, respectively. RESULTS: The addition of nitrate inhibited both endogenous and glucose-induced plaque pH lowering. The addition of glucose enhanced the production of nitrite from nitrate by about 3.3-fold. The addition of nitrite also inhibited endogenous plaque pH-lowering, but the addition of glucose promoted nitrite degradation by only about 1.1-fold. Nitrite-producing activity was positively correlated with age, but not with nitrite-degrading activity. CONCLUSION: This study revealed that nitrite was produced from nitrate and inhibited the pH-lowering activity of human plaque, which may contribute to caries control. Both nitrite-producing and -degrading activities occurred in human plaque, but no correlation was found between them. Furthermore, nitrite production was enhanced by glucose metabolism, which may function as a self-regulatory mechanism (resilience) to prevent excessive acidification by glucose metabolism.

  17. 緑茶カテキンEGCGによる歯周病関連細菌に対する抗菌効果,およびう蝕関連細菌に対する効果との比較

    樋口 真由, 安彦 友希, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　74　(増刊)　113-113　2024/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  18. Intracanal microbiome profiles of two apical periodontitis cases in one patient: A comparison with saliva and plaque profiles. International-journal

    Keiko Yamaki, Toru Tamahara, Jumpei Washio, Takuichi Sato, Ritsuko Shimizu, Satoru Yamada

    Clinical and experimental dental research　10　(2)　e862　2024/04

    DOI： 10.1002/cre2.862 　

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    OBJECTIVES: To determine the characteristics of the endodontic microbiome. MATERIAL AND METHODS: Saliva, plaque, and infected root canal wall dentin of two teeth suffering from apical periodontitis were harvested from a 58-year-old man. Bacterial DNA was extracted from each sample, and 16S rRNA gene analysis targeting the V3-V4 region was conducted on the Illumina MiSeq platform using QIIME2. The functional potential of the microbiomes was inferred using PICRUSt2. RESULTS: The four microbiomes were different in structure and membership, yet the nine most abundant metabolic pathways were common among them. The two endodontic microbiomes were more anaerobic, rich in Firmicutes, and scarce in Actinobacteriota and Proteobacteria, compared with saliva and plaque microbiomes. Their profiles were dissimilar despite their clinical and radiographic similarities. CONCLUSIONS: The endodontic microbiomes were anaerobic, rich in Firmicutes, scarce in Actinobacteriota and Proteobacteria, and considerably varied within an individual.

  19. Electrochemical difference between single‐ and poly‐crystalline LaF3 for a compact and low‐price fluoride ion sensor

    Kaito Kanda, Tetsuya Yamada, Yasuko Yanagida, Yuta Kubota, Nobuhiro Matsushita, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi

    Electroanalysis　2024/02/27

    Publisher: Wiley

    DOI： 10.1002/elan.202300318 　

    ISSN： 1040-0397

    eISSN： 1521-4109

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    Abstract Fluoride ion sensor electrodes that use polycrystalline LaF3 prepared by low‐temperature sintering can reduce sensor size and fabrication costs; however, decreasing the sintering temperature deteriorates sensor performance. Further, it is unclear why the performance of polycrystalline LaF3 prepared at low temperatures is inferior to that of the single‐crystal LaF3. Thus, in this study, we investigated factors that deteriorate sensor response under low sintering temperatures, such as solution penetration, fluorine conductivity, and ion exchange. To this end, LaF3 was prepared by changing the sintering temperature, and the electrical characteristics and sensor performance were evaluated and compared with those of the single‐crystal LaF3. The sensor performances of LaF3 sintered at 900 °C (−9.3 mV/dec) and 1000 °C (−5.8 mV/dec) were inferior to that of a single‐crystal LaF3 (−49.9 mV/dec). LaF3 prepared by sintering over 800 °C achieved fluorine removal and showed lower ion conductivity and ion exchange compared to that of the single‐crystal LaF3. LaF3 sintered at 700 °C did not show a sensor response because of solution penetration into the through‐pore. Our results confirmed that solution penetration, ion conductivity, and ion exchange influence the sensor performance of LaF3. Finally, we discussed approaches to achieve high sensor performance with LaF3 prepared by low‐temperature sintering. The proposed approaches are expected to contribute to expanding the utility of fluoride ion sensor electrodes with LaF3.

  20. Lactic Acid Bacteria in the Human Oral Cavity: Assessing Metabolic Functions Relevant to Oral Health and Disease. International-journal

    Jumpei Washio, Yuki Abiko, Takuichi Sato, Nobuhiro Takahashi

    Methods in molecular biology (Clifton, N.J.)　2851　151-172　2024

    DOI： 10.1007/978-1-0716-4096-8_14 　

    eISSN： 1940-6029

  21. Green Tea-Derived Catechins Suppress the Acid Productions of Streptococcus mutans and Enhance the Efficiency of Fluoride International-journal

    Sili Han, Jumpei Washio, Yuki Abiko, Linglin Zhang, Nobuhiro Takahashi

    Caries Research　57　(3)　255-264　2023/11/01

    DOI： 10.1159/000534055 　

    ISSN： 0008-6568

    eISSN： 1421-976X

  22. 細菌性代謝産物の酪酸が歯肉線維芽細胞の機能に与える影響

    大谷 栄毅, 鷲尾 純平, 國富 葵, 佐々木 詩織, 大橋 一正, 山田 聡, 高橋 信博

    日本歯周病学会会誌　65　(秋季特別)　132-132　2023/10

    Publisher: (NPO)日本歯周病学会

    ISSN： 0385-0110

    eISSN： 1880-408X

  23. 歯科訪問診療における水質管理方策の検討

    伏見 国拡, 山田 将博, 渡辺 隼, 鷲尾 純平, 高橋 信博, 江草 宏

    東北大学歯学雑誌　41/42　(2/1)　45-45　2023/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  24. 搾乳母乳と新生児口腔内細菌叢との連関

    涌井 杏奈, 河内 美帆, 佐野 拓人, 坂下 玲子, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    口腔衛生学会雑誌　73　(増刊)　153-153　2023/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  25. Fluoride ion sensor based on LaF&lt;sub&gt;3&lt;/sub&gt; nanocrystals prepared by low-temperature process

    Tetsuya Yamada, Kaito Kanda, Yasuko Yanagida, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi

    Journal of the Ceramic Society of Japan　131　(3)　31-36　2023/03/01

    Publisher: Ceramic Society of Japan

    DOI： 10.2109/jcersj2.22127 　

    ISSN： 1882-0743

    eISSN： 1348-6535

  26. Glycolysis-Related Gene Analyses Indicate That DEPDC1 Promotes the Malignant Progression of Oral Squamous Cell Carcinoma via the WNT/β-Catenin Signaling Pathway. International-journal

    Guangzhao Huang, Su Chen, Jumpei Washio, Grace Paka Lubamba, Nobuhiro Takahashi, Chunjie Li

    International journal of molecular sciences　24　(3)　2023/01/19

    DOI： 10.3390/ijms24031992 　

    ISSN： 1661-6596

    eISSN： 1422-0067

  27. Green Tea-Derived Catechins Suppress the Acid Productions of Streptococcus mutans and Enhance the Efficiency of Fluoride. International-journal

    Sili Han, Jumpei Washio, Yuki Abiko, Linglin Zhang, Nobuhiro Takahashi

    Caries research　57　(3)　255-264　2023

    DOI： 10.1159/000534055 　

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    Green tea-derived catechins, which can be divided into galloylated (epicatechin gallate: ECG, epigallocatechin gallate: EGCG) and non-galloylated (catechin: C, epicatechin: EC, epigallocatechin: EGC) catechins, are considered to be the main contributors to the caries control potential of green tea. In this study, we intended to compare the antimicrobial effects of these representative green tea-derived catechins and their combined effects with fluoride on the acid production and aggregation of Streptococcus mutans. The effects of different catechins on the growth, aggregation and acid production of S. mutans, and the combined effect of catechins and potassium fluoride (2 m<sc>m</sc> at pH 7.0, 0.3 m<sc>m</sc> at pH 5.5) on S. mutans acid production were measured by anaerobic culture, turbidity changes due to aggregation, and pH-stat methods. Molecular docking simulations were also performed to investigate the interactions between catechins and membrane-embedded enzyme II complex (EIIC), a component of the phosphoenolpyruvate-dependent phosphotransferase system (sugar uptake-related enzyme). ECG or EGCG at 1 mg/mL significantly inhibited the growth of S. mutans, induced bacterial aggregation, and decreased glucose-induced acid production (p &lt; 0.05). All catechins were able to bind to EIIC in silico, in the following order of affinity: EGCG, ECG, EGC, EC, and C. Furthermore, they enhanced the inhibitory effects of fluoride at pH 5.5 and significantly inhibited S. mutans acid production by 47.5-86.6% (p &lt; 0.05). These results suggest that both galloylated and non-galloylated catechins exhibit antimicrobial activity, although the former type demonstrates stronger activity, and that the caries control effects of green tea may be due to the combined effects of multiple components, such as catechins and fluoride. The detailed mechanisms underlying these phenomena and the in vivo effect need to be explored further.

  28. Profiling of the microbiota in the remaining sports drink and orange juice in plastic bottles after direct drinking. International-journal

    Miho Kawachi, Anna Wakui, Nagara Kaku, Nanase Takahashi, Shingo Maruyama, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of oral biosciences　64　(4)　437-444　2022/12

    DOI： 10.1016/j.job.2022.08.003 　

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    OBJECTIVES: The survival of bacteria in the sports drink and orange juice remaining in and at the mouth of bottles after direct drinking was examined after immediately drinking and incubation at 37°C for 24 h. METHODS: Nine healthy participants were asked to drink approximately 100 mL of a plastic bottled sports drink or orange juice. The samples were cultured anaerobically at 37°C for 7 days. Genomic DNA was extracted from the resulting individual colonies, and bacterial species were identified using 16S rRNA gene sequencing. RESULTS: The mean amount of bacteria in the remaining sports drink and orange juice, immediately after drinking, were (1.6 ± 2.3) × 103 colony-forming units (CFU)/mL and (2.9 ± 3.3) × 103 CFU/mL, respectively. Additionally, bacteria recovered from the mouths of the sports drink and orange juice bottles were (2.5 ± 5.5) × 104 CFU/mL and (5.8 ± 2.4) × 103 CFU/mL, respectively. Oral bacteria, such as Streptococcus, Actinomyces, Neisseria, and Rothia were found to be transferred in the sports drink and orange juice, and the bacteria were scarcely detected after incubation at 37°C for 24 h. CONCLUSIONS: The bacterial levels differed significantly from the previously reported levels in bottled tea 24 h after drinking, suggesting that remaining drinks with low pH levels can be preserved for a longer period.

  29. All‐solid‐state Fluoride Ion‐selective Electrode using LaF 3 Single Crystal with Poly(3,4‐ethylenedioxythiophene) as Solid Contact Layer

    Tetsuya Yamada, Kaito Kanda, Yasuko Yanagida, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi

    Electroanalysis　35　(4)　2022/10/21

    DOI： 10.1002/elan.202200103 　

    ISSN： 1040-0397

    eISSN： 1521-4109

  30. Investigation of drug resistance of caries-related streptococci to antimicrobial peptide GH12. International-journal

    Xinwei Li, Yufei Wang, Xuelian Jiang, Yuhao Zeng, Xinran Zhao, Jumpei Washio, Nobuhiro Takahashi, Linglin Zhang

    Frontiers in cellular and infection microbiology　12　991938-991938　2022/09/08

    DOI： 10.3389/fcimb.2022.991938 　

    eISSN： 2235-2988

  31. Profiling of the microbiota of breast milk before and after feeding with an artificial nipple International-journal

    Hiroto Sano, Anna Wakui, Miho Kawachi, Shingo Maruyama, Sachie Moriyama, Mayumi Nishikata, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Reiko Sakashita, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of Oral Biosciences　64　(4)　431-436　2022/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.job.2022.09.004 　

    ISSN： 1349-0079

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    OBJECTIVES: Breast milk is a valuable and useful source of nutrition; however, surplus milk is routinely discarded for hygiene reasons despite an unclear scientific basis. Here, we profiled the microbiota of expressed breast milk before and after feeding with an artificial nipple and examined the bacterial survival in breast milk stored at 4 °C. METHODS: Eleven mother-baby pairs were included in the study. Samples of expressed breast milk were collected before and after feeding with an artificial nipple and examined both immediately (0 h) and after storage for 3 and 12 h at 4 °C. Each sample was inoculated onto a blood agar plate and incubated anaerobically and aerobically at 37 °C. Genomic DNA was extracted from individual bacterial colonies, which were identified by 16S rRNA gene sequencing. RESULTS: Before feeding, the bacterial counts at 0 and 12 h were (1.4 ± 1.6) × 105 colony-forming units (CFU)/mL and (1.4 ± 0.6) × 105 CFU/mL, respectively. Staphylococcus (47.7% and 41.9%, respectively), Cutibacterium (20.7% and 36.0%, respectively), and Streptococcus (16.1% and 6.6%, respectively) were identified among the samples. In contrast, after feeding, the bacterial counts at 0 and 12 h were (2.7 ± 1.7) × 105 CFU/mL and (2.1 ± 2.5) × 105 CFU/mL, respectively. Staphylococcus (30.1% and 37.4%, respectively), Cutibacterium (11.7% and 31.7%, respectively), and Streptococcus (41.5% and 25.2%, respectively), were identified among the samples. CONCLUSIONS: Bacteria were present in the breast milk before feeding. Although the main component of the microbiota shifted from Staphylococcus to Streptococcus species after feeding, these results suggest that surplus expressed breast milk may be preserved safely in a refrigerator for at least 12 h after feeding with an artificial nipple.

  32. Microbiota profiles on the surface of non-woven fabric masks after wearing. International-journal

    Shingo Maruyama, Hiroto Sano, Anna Wakui, Miho Kawachi, Nagara Kaku, Nanase Takahashi, Misato Miyazawa, Takashi Abe, Aya Sato, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of oral biosciences　64　(3)　376-379　2022/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.job.2022.07.002 　

    ISSN： 1349-0079

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    This study aimed to characterize commensal microbiota on the skin before and after wearing masks, and to characterize the microbiota on the surface of used masks after 1 week of drying. From the 13 human subjects (age range, 19-26 years), mean bacterial concentrations of (6.1 ± 11.0) × 105 and (1.0 ± 1.4) × 106 colony-forming units (CFU)/mL were recovered from the skin of the buccal areas wiped with a sterile cotton swab before and after wearing non-woven fabric masks for 8 h, respectively. Furthermore (3.4 ± 4.9) × 104 CFU/mL of bacteria were recovered from the mask surfaces. The bacteria contained in the masks, which consisted mainly of Cutibacterium acnes and Staphylococcus epidermidis/aureus, virtually disappeared after drying the masks indoors for 1 week.

  33. Rewired Cellular Metabolic Profiles in Response to Metformin under Different Oxygen and Nutrient Conditions. International-journal

    Shan Liu, Jumpei Washio, Satoko Sato, Yuki Abiko, Yuta Shinohara, Yuri Kobayashi, Haruki Otani, Shiori Sasaki, Xiaoyi Wang, Nobuhiro Takahashi

    International journal of molecular sciences　23　(2)　2022/01/17

    DOI： 10.3390/ijms23020989 　

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    Metformin is a metabolic disruptor, and its efficacy and effects on metabolic profiles under different oxygen and nutrient conditions remain unclear. Therefore, the present study examined the effects of metformin on cell growth, the metabolic activities and consumption of glucose, glutamine, and pyruvate, and the intracellular ratio of nicotinamide adenine dinucleotide (NAD+) and reduced nicotinamide adenine dinucleotide (NADH) under normoxic (21% O2) and hypoxic (1% O2) conditions. The efficacy of metformin with nutrient removal from culture media was also investigated. The results obtained show that the efficacy of metformin was closely associated with cell types and environmental factors. Acute exposure to metformin had no effect on lactate production from glucose, glutamine, or pyruvate, whereas long-term exposure to metformin increased the consumption of glucose and pyruvate and the production of lactate in the culture media of HeLa and HaCaT cells as well as the metabolic activity of glucose. The NAD+/NADH ratio decreased during growth with metformin regardless of its efficacy. Furthermore, the inhibitory effects of metformin were enhanced in all cell lines following the removal of glucose or pyruvate from culture media. Collectively, the present results reveal that metformin efficacy may be regulated by oxygen conditions and nutrient availability, and indicate the potential of the metabolic switch induced by metformin as combinational therapy.

  34. Combined Treatment with Fluoride and Antimicrobial Peptide GH12 Efficiently Controls Caries in vitro and in vivo. International-journal

    Yufei Wang, Yuhao Zeng, Zening Feng, Zhongxu Li, Xuelian Jiang, Sili Han, Jumpei Washio, Nobuhiro Takahashi, Linglin Zhang

    Caries research　56　(5-6)　524-534　2022

    DOI： 10.1159/000528057 　

    ISSN： 0008-6568

    eISSN： 1421-976X

  35. Facile Fabrication of All-solid-state Ion-selective Electrodes by Laminating and Drop-casting for Multi-sensing

    Shingo Okubo, Yoshihisa Ozeki, Tetsuya Yamada, Kosuke Saito, Noboru Ishihara, Yasuko Yanagida, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi

    Electrochemistry　90　(7)　2022

    DOI： 10.5796/ELECTROCHEMISTRY.22-00020 　

    ISSN： 1344-3542

    eISSN： 2186-2451

  36. A detection and analysis of Fusobacterium utilizing changes in the magnetic properties of magnetic nanoparticlesantibody-antigen aggregates

    S. Yabukami, T. Murayama, S. Takahashi, S. Ohno, J. Washio, N. Takahashi

    IEEE Transactions on Magnetics　1-1　2022

    Publisher: Institute of Electrical and Electronics Engineers (IEEE)

    DOI： 10.1109/tmag.2022.3161508 　

    ISSN： 0018-9464

    eISSN： 1941-0069

  37. Influence of alcohol sensitivity on bone metastases and skeletal-related events in primary operable breast cancer: A retrospective cohort study. International-journal

    Naoko Tanda, Hiroshi Tada, Jumpei Washio, Nobuhiro Takahashi, Takanori Ishida, Takeyoshi Koseki

    PloS one　17　(6)　e0269335　2022

    DOI： 10.1371/journal.pone.0269335 　

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    BACKGROUND: Bone metastases in breast cancer patients are a common concern for medical doctors and dentists. Bone-modifying agents, which are necessary to prevent skeletal-related events (SREs), are associated with osteonecrosis of the jaw as an adverse side effect. Hypersensitivity to alcohol is an unfavorable response caused by deficiency of aldehyde dehydrogenase-2 (ALDH2) activity. Inactive ALDH2 is associated with osteoporosis, but its influence on bone metastases is unclear. The aim of our study was to evaluate the effects of alcohol sensitivity on bone metastases and SREs in primary operable breast cancer patients. METHODS: We retrospectively analyzed patients who were administered docetaxel, an anti-tumor agent, for histologically diagnosed breast cancer between April 2004 and September 2015. Alcohol sensitivity was assessed based on medical records of hypersensitivity to alcohol. The primary endpoint was time to bone metastases and the secondary endpoint was time to first SRE from the initial docetaxel administration. Data were stratified by alcohol sensitivity and tumor stages, and differences were estimated by the Kaplan-Meier method. Prognostic risk factors were analyzed by the multivariate Cox proportional hazards model. RESULTS: The median follow-up period of patients with high sensitivity to alcohol (n = 45) was 54 months and that for those with low sensitivity (n = 287) was 64 months. Stratification by alcohol sensitivity revealed that tumor stage exhibited significant correlations with the cumulative incidence of bone metastases in low-sensitivity patients; however, no differences were found in high-sensitivity patients. In multivariate analysis, alcohol sensitivity was a significant prognostic risk factor for bone metastases (HR 2.721, 95% CI 1.268-5.841, P = 0.010). CONCLUSION: Alcohol sensitivity may be a prognostic risk factor for bone metastases. More detailed genetic investigations and metabolic analyses are needed.

  38. 口腔Neisseria属による亜硝酸産生活性と環境因子による影響(The Nitrite producing activity of oral Neisseria and the effect of environmental factors)

    鷲尾 純平, 江副 和子, 佐藤 聡子, 安彦 友希, 小峰 英也, 柴田 怜, 田花 航平, 毛 雪竹, 高橋 信博

    Journal of Oral Biosciences Supplement　2021　208-208　2021/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  39. 若手研究者による口腔微生物叢に関するチャレンジレポート 口腔内細菌によるエタノールからのアセトアルデヒド産生に関する研究 口腔内環境因子の影響とエタノール濃度(The Challenge Reports on Oral Microbiota by Young Researchers The study on the production of acetaldehyde from ethanol by oral bacteria: Effects of oral environmental factors and ethanol concentration)

    Tagaino R, Washio J, Sasaki K, Takahashi N

    Journal of Oral Biosciences Supplement　2021　103-103　2021/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  40. Bifacial biological effects of ethanol: acetaldehyde production by oral Streptococcus species and the antibacterial effects of ethanol against these bacteria. International-journal

    Ryo Tagaino, Jumpei Washio, Haruki Otani, Keiichi Sasaki, Nobuhiro Takahashi

    Journal of oral microbiology　13　(1)　1937884-1937884　2021/06/09

    DOI： 10.1080/20002297.2021.1937884 　

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    Background:Many previous studies have focused on the acetaldehyde produced from ethanol by oral bacteria as a risk factor for oral cancer. Most of these studies involved low ethanol concentrations (ca. 10 mM), but oral bacteria are exposed to a wide range of ethanol concentrations (100-10,000 mM) when alcoholic beverages are consumed. In contrast, ethanol is widely used at high concentrations (> 5,000 mM) as an antiseptic/disinfectant, suggesting that ethanol has bifacial biological effects; i.e. it acts as both a metabolic substrate for bacterial acetaldehyde production and an antimicrobial agent. Materials and methods:We examined the acetaldehyde production from ethanol by oral streptococci and the effects of ethanol exposure on the growth and viability of these bacteria at a wide range of ethanol concentrations (10-10,000 mM). Results:Acetaldehyde production was the highest at an ethanol concentration of 2,000 mM (2.1-48-fold higher than that seen at an ethanol concentration of 10 mM). Bacterial growth was inhibited by > 1,000 mM of ethanol, and the bacteria did not seem viable in the presence of > 5,000 mM of ethanol, although they still produced acetaldehyde. Conclusion:Ethanol has bifacial biological effects, and the concentration ranges of these effects overlap.

  41. Profiling system of oral microbiota utilizing polymerase chain reaction-restriction fragment length polymorphism analysis. International-journal

    Hiroto Sano, Anna Wakui, Miho Kawachi, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of oral biosciences　63　(3)　292-297　2021/06/07

    DOI： 10.1016/j.job.2021.05.003 　

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    OBJECTIVES: Profiling of oral microbiota has traditionally been performed using conventional methods. These methods are relatively time-consuming and labor-intensive. Metagenomic analysis of oral microbiota using high-speed next-generation sequencing is a highly promising technology. However, it is expensive. This study sought to develop a simple and cost-effective profiling method for oral microbiota using 16S rRNA gene polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of PCR-amplified 16S ribosomal RNA genes. METHODS: Oral isolates of 59 bacterial species from human saliva, including Streptococcus, Actinomyces, and Veillonella, were cultured anaerobically on CDC Anaerobe 5% sheep blood agar plates. Genomic DNA was extracted from single colonies and 16S rRNA genes were PCR-amplified using the 27F and 1492R universal primers. The PCR products were purified and characterized by single digestion with HpaII restriction endonuclease. 16S rRNA gene sequences were obtained from the GenBank database, and the expected restriction profiles were compared with the RFLP patterns obtained from agarose gel electrophoresis. RESULTS: Sixty-five RFLP patterns were obtained from 27 genera and 59 species. The expected fragment sizes of these species were calculated based on GenBank 16S rRNA gene sequences. Fifty-nine patterns were obtained from the analysis of GenBank sequences. The RFLP patterns produced with HpaII distinguished many oral bacterial species. RFLP patterns enabling identification of oral bacteria were generated. The 16S rRNA gene PCR-RFLP analysis did not require expensive equipment and reagents and was cost-effective. CONCLUSION: PCR-RFLP analysis based on 16S rRNA genes could be an alternative method for oral microbiota analysis in smaller laboratories.

  42. Effectiveness and safety of a new dental plaque removal device utilizing micro mist spray for removing oral biofilm in vitro. International-journal

    Hiroki Hihara, Ryo Tagaino, Jumpei Washio, Kittipong Laosuwan, Dimas Prasetianto Wicaksono, Kuniyuki Izumita, Rie Koide, Nobuhiro Takahashi, Keiichi Sasaki

    BMC oral health　21　(1)　286-286　2021/06/04

    DOI： 10.1186/s12903-021-01647-4 　

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    BACKGROUND: Removal of oral biofilm from the oral mucosa is essential for preventing risk of respiratory and gastrointestinal infection in elderly people. Currently, no device is available which can remove oral biofilm from oral mucosa effectively and safely. Therefore, the effectiveness and safety of the Micro Scale Mist UNIT (MSM-UNIT), a newly developed dental plaque removal device utilizing high speed sprays of fine water droplets, were evaluated for biofilm removal, including the rate and surface roughness for simulated tooth surface and mucous membrane. METHODS: Simulated tooth and oral mucosa coated with an artificial biofilm of Streptococcus mutans were used for evaluation of effectiveness, with uncoated substrates as the controls. The MSM-UNIT and a conventional air ablation device were operated under recommended instructions. The effectiveness was evaluated from the rate of removal of the biofilm, and the safety was evaluated from the damage observed by scanning electron microscope and surface roughness. RESULTS: The biofilm removal rate of the MSM-UNIT was significantly higher than that of AIRFLOW. Little damage was observed in the area treated by the MSM-UNIT. The surface roughness of the MSM-UNIT treated area on simulated tooth surface and oral mucosa showed no significant difference to the control area. In contrast, cracks and powder were observed in the area treated by AIRFLOW. In particular, the surface roughness of the AIRFLOW treated area for Toughsilon was significantly larger than that of the control. CONCLUSIONS: The MSM-UNIT could be used safely and effectively for removing biofilm not only on simulated tooth surfaces but also simulated mucous membrane. The MSM-UNIT has no harmful effect on teeth or oral mucosa, and may be used for comprehensive oral care for patients during nursing care and the perioperative period.

  43. Bacterial concentration and composition in liquid baby formula and a baby drink consumed with an artificial nipple. International-journal

    Anna Wakui, Hiroto Sano, Miho Kawachi, Ayaka Aida, Yuta Takenaka, Akane Yonezawa, Nana Nakahata, Sachie Moriyama, Mayumi Nishikata, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Reiko Sakashita, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Journal of oral biosciences　63　(2)　161-168　2021/06

    DOI： 10.1016/j.job.2021.03.001 　

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    OBJECTIVES: To clarify the characteristics and growth of bacteria that may infiltrate liquid baby formula during feeding and after storage for more than 3 h, the transfer of oral bacteria through artificial nipples, and bacterial survival in liquid baby formula and a baby drink were examined immediately after drinking and after storage at 4 °C for 12 h and 24 h. METHODS: Thirteen human subjects (aged 19-24 years) were asked to drink approximately 50 mL of liquid baby formula and a baby drink, via the artificial nipple of a baby bottle. Samples of the remaining liquid after storage at 4 °C for 12 h and 24 h were inoculated onto blood agar plates and incubated anaerobically at 37 °C for 7 days. Genomic DNA was extracted from individual colonies, and the bacterial species were identified by 16S rRNA gene sequencing. RESULTS: The mean concentrations of bacteria in the liquid baby formula were (2.6 ± 2.8) × 104 and (4.1 ± 6.6) × 104 colony-forming unit/mL after storage at 4 °C for 12 h and 24 h, respectively. Streptococcus (43.2%), Veillonella (9.3%), and Schaalia (8.2%) species were recovered from the remaining liquid baby formula after storage at 4 °C for 12 h. In contrast, no bacteria were detected in the remaining baby drink after storage at 37 °C for 24 h. CONCLUSIONS: The levels of bacteria immediately after drinking and after storage at 4 °C for 12 h or 24 h were similar, suggesting that remaining liquid baby formula may be preserved safely in a refrigerator for more than 3 h.

  44. Profiling of Microbiota at the Mouth of Bottles and in Remaining Tea after Drinking Directly from Plastic Bottles of Tea. International-journal

    Anna Wakui, Hiroto Sano, Yuka Hirabuki, Miho Kawachi, Ayaka Aida, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Dentistry journal　9　(6)　2021/05/21

    DOI： 10.3390/dj9060058 　

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    It has been speculated that oral bacteria can be transferred to tea in plastic bottles when it is drunk directly from the bottles, and that the bacteria can then multiply in the bottles. The transfer of oral bacteria to the mouth of bottles and bacterial survival in the remaining tea after drinking directly from bottles were examined immediately after drinking and after storage at 37 °C for 24 h. Twelve healthy subjects (19 to 23 years of age) were asked to drink approximately 50 mL of unsweetened tea from a plastic bottle. The mouths of the bottles were swabbed with sterile cotton, and the swabs and the remaining tea in the bottles were analyzed by anaerobic culture and 16S rRNA gene sequencing. Metagenomic analysis of the 16S rRNA gene was also performed. The mean amounts of bacteria were (1.8 ± 1.7) × 104 colony-forming units (CFU)/mL and (1.4 ± 1.5) × 104 CFU/mL at the mouth of the bottles immediately after and 24 h after drinking, respectively. In contrast, (0.8 ± 1.6) × 104 CFU/mL and (2.5 ± 2.6) × 106 CFU/mL were recovered from the remaining tea immediately after and 24 h after drinking, respectively. Streptococcus (59.9%) were predominant at the mouth of the bottles immediately after drinking, followed by Schaalia (5.5%), Gemella (5.5%), Actinomyces (4.9%), Cutibacterium (4.9%), and Veillonella (3.6%); the culture and metagenomic analyses showed similar findings for the major species of detected bacteria, including Streptococcus (59.9%, and 10.711%), Neisseria (1.6%, and 24.245%), Haemophilus (0.6%, and 15.658%), Gemella (5.5%, and 0.381%), Cutibacterium (4.9%, and 0.041%), Rothia (2.6%, and 4.170%), Veillonella (3.6%, and 1.130%), Actinomyces (4.9%, and 0.406%), Prevotella (1.6%, and 0.442%), Fusobacterium (1.0%, and 0.461%), Capnocytophaga (0.3%, and 0.028%), and Porphyromonas (1.0%, and 0.060%), respectively. Furthermore, Streptococcus were the most commonly detected bacteria 24 h after drinking. These findings demonstrated that oral bacteria were present at the mouth of the bottles and in the remaining tea after drinking.

  45. 集団微生物学と細菌バイオフィルム研究の前線 口腔と全身の健康に関わる口腔バイオフィルムの代謝機能(Metabolic function of oral biofilm involved in oral and systemic health)

    高橋 信博, 鷲尾 純平

    日本細菌学雑誌　76　(1)　31-31　2021/02

    Publisher: 日本細菌学会

    ISSN： 0021-4930

    eISSN： 1882-4110

  46. Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions. International-journal

    Yuta Shinohara, Jumpei Washio, Yuri Kobayashi, Yuki Abiko, Keiichi Sasaki, Nobuhiro Takahashi

    PloS one　16　(10)　e0254966　2021

    DOI： 10.1371/journal.pone.0254966 　

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    OBJECTIVE: The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore, we investigated the effects of oxygen concentration fluctuations on the glucose metabolism of cancer cells. METHODS: The glucose metabolism of oral squamous cell carcinoma (HSC-2 and HSC-3) and normal epithelial (HaCaT) cells cultured under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions was measured using a pH-stat system under normoxic or hypoxic conditions. The acidic end-products and reactive oxygen species (ROS) generated by glucose metabolism were also measured. RESULTS: Under normoxic conditions, the metabolic activity of hypoxically cultured cancer cells was significantly increased, and the production of acids other than lactate was upregulated, while the normal cells did not respond to rapid increases in oxygen levels. ROS production was higher in normoxic conditions in all cells, especially the hypoxically cultured HSC-3 cells. CONCLUSIONS: Rapid increases in oxygen levels might enhance the glucose metabolism of hypoxically cultured cancer cells by mainly activating the TCA cycle and electron transport system, which might activate cancer cells through the ATP and ROS generation.

  47. Green Tea-Derived Epigallocatechin Gallate Inhibits Acid Production and Promotes the Aggregation of Streptococcus mutans and Non-Mutans Streptococci. International-journal

    Sili Han, Yuki Abiko, Jumpei Washio, Yufang Luo, Linglin Zhang, Nobuhiro Takahashi

    Caries research　55　(3)　205-214　2021

    DOI： 10.1159/000515814 　

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    It has been suggested that green tea-derived epigallocatechin gallate (EGCG), which has antimicrobial properties, might help prevent dental caries. However, the detailed properties of EGCG remain unclear. In this study, the antimicrobial properties of EGCG were evaluated by examining its bactericidal activity, its inhibitory effects against bacterial growth, acid production, acidic end-product formation, and sugar uptake (phosphoenolpyruvate-dependent phosphotransferase system, PEP-PTS activity), and its effects on bacterial aggregation, using monocultured planktonic cells of Streptococcus mutans and non-mutans streptococci. Coincubating S. mutans with EGCG (1 mg/mL) for 4 h had no bactericidal effects, while it decreased the growth and acid production of S. mutans by inhibiting the activity of the PEP-PTS. EGCG (2 mg/mL) caused rapid bacterial cell aggregation and had reduced the optical density of S. mutans cell suspension by 86.7% at pH 7.0 and 90.7% at pH 5.5 after 2 h. EGCG also reduced the acid production of non-mutans streptococci, including S. sanguinis, S. gordonii, and S. salivarius, and promoted the aggregation of these non-mutans streptococci. Furthermore, these antimicrobial effects of short-term EGCG treatment persisted in the presence of saliva. These results suggest that EGCG might have short-term antibacterial effects on caries-associated streptococci in the oral cavity.

  48. Nitrite-producing oral microbiome in adults and children. International-journal

    Yuria Sato-Suzuki, Jumpei Washio, Dimas Prasetianto Wicaksono, Takuichi Sato, Satoshi Fukumoto, Nobuhiro Takahashi

    Scientific reports　10　(1)　16652-16652　2020/10/06

    DOI： 10.1038/s41598-020-73479-1 　

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    Recently, it was suggested that the nitrite (NO2-) produced from NO3- by oral bacteria might contribute to oral and general health. Therefore, we aimed to clarify the detailed information about the bacterial NO2-production in the oral biofilm. Dental plaque and tongue-coating samples were collected, then the NO2-producing activity was measured. Furthermore, the composition of the NO2--producing bacterial population were identified using the Griess reagent-containing agar overlay method and molecular biological method. NO2--producing activity per mg wet weight varied among individuals but was higher in dental plaque. Additionally, anaerobic bacteria exhibited higher numbers of NO2--producing bacteria, except in the adults' dental plaque. The proportion of NO2--producing bacteria also varied among individuals, but a positive correlation was found between NO2--producing activity and the number of NO2--producing bacteria, especially in dental plaque. Overall, the major NO2--producing bacteria were identified as Actinomyces, Schaalia, Veillonella and Neisseria. Furthermore, Rothia was specifically detected in the tongue coatings of children. These results suggest that dental plaque has higher NO2--producing activity and that this activity depends not on the presence of specific bacteria or the bacterial compositions, but on the number of NO2--producing bacteria, although interindividual differences were detected.

  49. Nitrite Production from Nitrate and Its Link with Lactate Metabolism in Oral Veillonella spp. International-journal

    Dimas Prasetianto Wicaksono, Jumpei Washio, Yuki Abiko, Hitomi Domon, Nobuhiro Takahashi

    Applied and environmental microbiology　86　(20)　2020/10/01

    DOI： 10.1128/AEM.01255-20 　

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    Veillonella species are among the major anaerobes in the oral cavity and are frequently detected in both caries lesions and healthy oral microbiomes. They possess the ability to utilize lactate and convert nitrate (NO3-) into nitrite (NO2-). Recently, interest in NO2- has increased rapidly because of its beneficial effects on oral and general health; i.e., it inhibits the growth and metabolism of oral pathogenic bacteria, such as Streptococcus mutans, and lowers systemic blood pressure. However, there is only limited information about the biochemical characteristics of NO2- production by Veillonella species. We found that NO3- did not inhibit the growth of Veillonella atypica or Veillonella parvula, and it inhibited the growth of Streptococcus mutans only at a high concentration (100 mM). However, NO2- inhibited the growth of Streptococcus mutans at a low concentration (0.5 mM), while a higher concentration of NO2- (20 mM) was needed to inhibit the growth of Veillonella species. NO2- production by Veillonella species was increased by environmental factors (lactate, acidic pH, and anaerobic conditions) and growth conditions (the presence of NO3- or NO2-) and was linked to anaerobic lactate metabolism. A stoichiometric evaluation revealed that NO3- is reduced to NO2- by accepting reducing power derived from the oxidization of lactate. These findings suggest that the biochemical characteristics of NO2- production from NO3- and its linkage with lactate metabolism in oral Veillonella species may play a key role in maintaining good oral and general health.IMPORTANCE The prevalence of dental caries is still high around the world. Dental caries is initiated when the teeth are exposed to acid, such as lactic acid, produced via carbohydrate metabolism by acidogenic microorganisms. Veillonella species, which are among the major oral microorganisms, are considered to be beneficial bacteria due to their ability to convert lactic acid to weaker acids and to produce NO2- from NO3-, which is thought to be good for both oral and general health. Therefore, it is clear that there is a need to elucidate the biochemical characteristics of NO2- production in Veillonella species. The significance of our research is that we have found that lactate metabolism is linked to NO2- production by Veillonella species in the environment found in the oral cavity. This study suggests that Veillonella species are potential candidates for maintaining oral and general health.

  50. Sugar Metabolism of Scardovia wiggsiae, a Novel Caries-Associated Bacterium International-journal Peer-reviewed

    Mai Kameda, Yuki Abiko, Jumpei Washio, Anne C.R. Tanner, Christine A. Kressirer, Itaru Mizoguchi, Nobuhiro Takahashi

    Frontiers in Microbiology　11　479-479　2020/03/25

    DOI： 10.3389/fmicb.2020.00479 　

    eISSN： 1664-302X

  51. Use of cellulose nanofibers as a denture immersing solution. Peer-reviewed

    Wei Zou, Guang Hong, Yukiko Yamazaki, Kazuma Takase, Toru Ogawa, Jumpei Washio, Nobuhiro Takahashi, Keiichi Sasaki

    Dental materials journal　39　(1)　80-88　2020/01/31

    DOI： 10.4012/dmj.2018-388 　

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    The purpose of this study was to investigate the influence of cellulose nanofibers (CNF) solution on the mechanical and biological properties of denture base resins (DBR). Two types of CNFs obtained from bamboo (BB) and needle-leaved (NB) trees were used in this study. We prepared 18 different CNF solutions based on their fibrillation (A-low, B-middle, and C-high) and concentration (0.05, 0.10, and 0.20 wt%). A heat-polymerized acrylic resin was used as DBR. The contact angles for each specimen were measured after immersion. The flexural properties of the immersed specimens, and the biological properties of the CNF solutions were examined. Specimens immersed in CNF-NB-C-0.05 wt% solution presented with the lowest contact angles. Specimens in CNF-NB-C and CNF-BB-A groups showed higher flexural modulus values. No cytotoxic or antimicrobial effects were observed for the CNF solutions. This study suggest that CNF solution may improve the surface wettability of the DBR without affecting its flexural property.

  52. Erratum to: Use of cellulose nanofibers as a denture immersing solution.

    Wei Zou, Guang Hong, Yukiko Yamazaki, Kazuma Takase, Toru Ogawa, Jumpei Washio, Nobuhiro Takahashi, Keiichi Sasaki

    Dental materials journal　39　(2)　327-327　2020

    DOI： 10.4012/dmj.2018-388-e 　

  53. Metabolic property of acetaldehyde production from ethanol and glucose by oral Streptococcus and Neisseria International-journal Peer-reviewed

    Ryo Tagaino, Jumpei Washio, Yuki Abiko, Naoko Tanda, Keiichi Sasaki, Nobuhiro Takahashi

    Scientific Reports　9　(1)　10446-10446　2019/12/01

    DOI： 10.1038/s41598-019-46790-9 　

    eISSN： 2045-2322

  54. PCR-RFLP法を用いた口腔内細菌叢解析(プロファイリング)システム Peer-reviewed

    佐野 拓人, 涌井 杏奈, 八巻 惠子, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    Journal of Oral Biosciences Supplement　2019　256-256　2019/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  55. Draft genome sequences of two veillonella tobetsuensis clinical isolates from intraoperative bronchial fluids of elderly patients with pulmonary carcinoma International-journal Peer-reviewed

    Izumi Mashima, Tohru Miyoshi-Akiyama, Junko Tomida, Ryo Kutsuna, Jumpei Washio, Nobuhiro Takahashi, Futoshi Nakazawa, Takuichi Sato, Yoshiaki Kawamura

    Microbiology Resource Announcements　8　(38)　2019/09/19

    DOI： 10.1128/MRA.00397-19 　

    eISSN： 2576-098X

  56. Acidogenic potential of oral bifidobacterium and its high fluoride tolerance International-journal Peer-reviewed

    Ayumi Manome, Yuki Abiko, Junko Kawashima, Jumpei Washio, Satoshi Fukumoto, Nobuhiro Takahashi

    Frontiers in Microbiology　10　(MAY)　1099-1099　2019

    DOI： 10.3389/fmicb.2019.01099 　

    eISSN： 1664-302X

  57. Profiling of microbiota in liquid baby formula consumed with an artificial nipple. Peer-reviewed

    Hiroto Sano, Anna Wakui, Miho Kawachi, Rito Kato, Sachie Moriyama, Mayumi Nishikata, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Reiko Sakashita, Junko Tomida, Yoshiaki Kawamura, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

    Biomedical research (Tokyo, Japan)　40　(4)　163-168　2019

    DOI： 10.2220/biomedres.40.163 　

    ISSN： 0388-6107

    More details Close

    It is suspected that oral bacteria are transferred to the liquid baby formula through the artificial nipple and multiply in the bottle after feeding. In the present study, in order to understand the influence of bacteria on liquid baby formula after feeding, the transfer of oral bacteria through artificial nipples and their survival in liquid baby formula were examined immediately after drinking as well as after storage at 4°C for 3 h. Four healthy human subjects (20-23 years old) were asked to drink liquid baby formula (Aptamil®, ca. 50 mL) from baby bottles using artificial nipples. Samples of the liquid baby formula (immediately after drinking and 3 h later) were inoculated onto blood agar plates and incubated anaerobically at 37°C for 7 days. Salivary samples from each subject and 6 newborn infants were also cultured. Genomic DNA was extracted from individual colonies, and bacterial species were identified by 16S rRNA gene sequencing. The mean amounts of bacteria (CFU/mL) were (3.2 ± 3.0) ×104 and (3.4 ± 3.3) ×104 immediately after drinking and 3 h later, respectively. Streptococcus (41.6 and 40.5%), Actinomyces (24.3 and 21.5%) and Veillonella (16.2 and 11.0%) were recovered from the samples immediately after drinking and 3 h later, respectively. On the other hand, Streptococcus (38.9%), Actinomyces (17.1%), Neisseria (9.1%), Prevotella (6.9%), Rothia (6.9%) and Gemella (5.1%) were predominant in the saliva of adult subjects, and Streptococcus (65.2%), Staphylococcus (18.5%), Gemella (8.2%) and Rothia (5.4%) were predominant in the saliva of infant subjects. From these findings, oral bacteria, e.g., Streptococcus, Gemella and Rothia, were found to transfer into the liquid baby formula through artificial nipples, and the bacterial composition in the remaining liquid baby formula was found to resemble that of human saliva. The bacterial levels were similar between immediately after drinking and when stored at 4°C for 3 h, suggesting that the remaining liquid baby formula may be preserved in a refrigerator for a specified amount of time.

  58. Professional oral care reduces carcinogenic acetaldehyde levels in mouth air of perioperative esophageal cancer patients: A prospective comparative study Peer-reviewed

    Naoko Tanda, Jumpei Washio, Takashi Kamei, Kohei Akazawa, Nobuhiro Takahashi, Takeyoshi Koseki

    Tohoku Journal of Experimental Medicine　249　(1)　75-83　2019

    DOI： 10.1620/tjem.249.75 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  59. Real-time monitoring system for evaluating the acid-producing activity of oral squamous cell carcinoma cells at different environmental pH International-journal Peer-reviewed

    Hiromitsu Morishima, Jumpei Washio, Jun Kitamura, Yuta Shinohara, Tetsu Takahashi, Nobuhiro Takahashi

    SCIENTIFIC REPORTS　7　(1)　10092-10092　2017/08

    DOI： 10.1038/s41598-017-10893-y 　

    ISSN： 2045-2322

  60. Inhibitory Effects of Nitrite on Acid Production in Dental Plaque in Children International-journal Peer-reviewed

    Yuji Yamamoto, Jumpei Washio, Koichi Shimizu, Koei Igarashi, Nobuhiro Takahashi

    ORAL HEALTH & PREVENTIVE DENTISTRY　15　(2)　153-156　2017/04

    DOI： 10.3290/j.ohpd.a37926 　

    ISSN： 1602-1622

    eISSN： 1757-9996

  61. pH Response and Tooth Surface Solubility at the Tooth/Bacteria Interface International-journal Peer-reviewed

    Gen Mayanagi, Koei Igarashi, Jumpei Washio, Nobuhiro Takahashi

    CARIES RESEARCH　51　(2)　160-166　2017

    DOI： 10.1159/000454781 　

    ISSN： 0008-6568

    eISSN： 1421-976X

  62. Metabolomic studies of oral biofilm, oral cancer, and beyond International-journal

    Jumpei Washio, Nobuhiro Takahashi

    International Journal of Molecular Sciences　17　(6)　2016/06

    DOI： 10.3390/ijms17060870 　

    ISSN： 1661-6596

    More details Close

    Oral diseases are known to be closely associated with oral biofilm metabolism, while cancer tissue is reported to possess specific metabolism such as the ‘Warburg effect’. Metabolomics might be a useful method for clarifying the whole metabolic systems that operate in oral biofilm and oral cancer, however, technical limitations have hampered such research. Fortunately, metabolomics techniques have developed rapidly in the past decade, which has helped to solve these difficulties. In vivo metabolomic analyses of the oral biofilm have produced various findings. Some of these findings agreed with the in vitro results obtained in conventional metabolic studies using representative oral bacteria, while others differed markedly from them. Metabolomic analyses of oral cancer tissue not only revealed differences between metabolomic profiles of cancer and normal tissue, but have also suggested a specific metabolic system operates in oral cancer tissue. Saliva contains a variety of metabolites, some of which might be associated with oral or systemic disease; therefore, metabolomics analysis of saliva could be useful for identifying disease-specific biomarkers. Metabolomic analyses of the oral biofilm, oral cancer, and saliva could contribute to the development of accurate diagnostic, techniques, safe and effective treatments, and preventive strategies for oral and systemic diseases.

  63. Metabolomic Studies of Oral Biofilm, Oral Cancer, and Beyond International-journal Peer-reviewed

    Jumpei Washio, Nobuhiro Takahashi

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES　17　(6)　2016/06

    DOI： 10.3390/ijms17060870 　

    ISSN： 1422-0067

  64. PCR-dipstick DNA chromatography for profiling of a subgroup of caries-associated bacterial species in plaque from healthy coronal surfaces and periodontal pockets Peer-reviewed

    Lingyang Tian, Takuichi Sato, Kousuke Niwa, Mitsuo Kawase, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi

    BIOMEDICAL RESEARCH-TOKYO　37　(1)　29-36　2016

    DOI： 10.2220/biomedres.37.29 　

    ISSN： 0388-6107

    eISSN： 1880-313X

  65. Amino acid composition and amino acid-metabolic network in supragingival plaque Peer-reviewed

    Jumpei Washio, Tamaki Ogawa, Keisuke Suzuki, Yosuke Tsukiboshi, Motohiro Watanabe, Nobuhiro Takahashi

    BIOMEDICAL RESEARCH-TOKYO　37　(4)　251-257　2016

    DOI： 10.2220/biomedres.37.251 　

    ISSN： 0388-6107

    eISSN： 1880-313X

  66. Oral microbiota in crevices around dental implants: profiling of oral biofilm Invited

    Sato T, Kawamura Y, Yamaki K, Ishida N, Tian L, Takeuchi Y, Hashimoto K, Abiko Y, Mayanagi G, Washio J, Matsuyama J, Takahashi N

    Interface Oral Health Science 2014　45-50　2015/09

  67. Real-time monitoring of the metabolic activity of periodontopathic bacteria International-journal Peer-reviewed

    Kazuko Ishiguro, Jumpei Washio, Keiichi Sasaki, Nobuhiro Takahashi

    JOURNAL OF MICROBIOLOGICAL METHODS　115　22-26　2015/08

    DOI： 10.1016/j.mimet.2015.05.015 　

    ISSN： 0167-7012

    eISSN： 1872-8359

  68. Analysis of ketone bodies in exhaled breath and blood of ten healthy Japanese at OGTT using a portable gas chromatograph International-journal Peer-reviewed

    Naoko Tanda, Yoshinori Hinokio, Jumpei Washio, Nobuhiro Takahashi, Takeyoshi Koseki

    JOURNAL OF BREATH RESEARCH　8　(4)　046008-046008　2014/12

    DOI： 10.1088/1752-7155/8/4/046008 　

    ISSN： 1752-7155

    eISSN： 1752-7163

  69. Glucose and glutamine metabolism in oral squamous cell carcinoma: insight from a quantitative metabolomic approach International-journal Peer-reviewed

    Tamaki Ogawa, Jumpei Washio, Tetsu Takahashi, Seishi Echigo, Nobuhiro Takahashi

    ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY　118　(2)　218-225　2014/08

    DOI： 10.1016/j.oooo.2014.04.003 　

    ISSN： 2212-4403

    eISSN： 1528-395X

  70. Effects of pH and Lactate on Hydrogen Sulfide Production by Oral Veillonella spp. International-journal Peer-reviewed

    Jumpei Washio, Yuko Shimada, Masakazu Yamada, Ryouichi Sakamaki, Nobuhiro Takahashi

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY　80　(14)　4184-4188　2014/07

    DOI： 10.1128/AEM.00606-14 　

    ISSN： 0099-2240

    eISSN： 1098-5336

  71. Effect of fluoride-releasing restorative materials on bacteria-induced pH fall at the bacteria-material interface: An in vitro model study International-journal Peer-reviewed

    Gen Mayanagi, Koei Igarashi, Jumpei Washio, Hitomi Domon-Tawaraya, Nobuhiro Takahashi

    JOURNAL OF DENTISTRY　42　(1)　15-20　2014/01

    DOI： 10.1016/j.jdent.2013.11.006 　

    ISSN： 0300-5712

    eISSN： 1879-176X

  72. Fluoride-sensitivity of growth and acid production of oral Actinomyces: comparison with oral Streptococcus International-journal Peer-reviewed

    Junko Kawashima, Kazuko Nakajo, Jumpei Washio, Gen Mayanagi, Hidetoshi Shimauchi, Nobuhiro Takahashi

    MICROBIOLOGY AND IMMUNOLOGY　57　(12)　797-804　2013/12

    DOI： 10.1111/1348-0421.12098 　

    ISSN： 0385-5600

    eISSN： 1348-0421

  73. A high-sensitive and non-radioisotopic fluorescence dye method for evaluating bacterial adhesion to denture materials Peer-reviewed

    Yoko Sakuma, Jumpei Washio, Keiichi Sasaki, Nobuhiro Takahashi

    DENTAL MATERIALS JOURNAL　32　(4)　585-591　2013/08

    DOI： 10.4012/dmj.2013-060 　

    ISSN： 0287-4547

    eISSN： 1881-1361

  74. Metabolomic approach to oral biofilm characterization-A future direction of biofilm research

    Nobuhiro Takahashi, Jumpei Washio, Gen Mayanagi

    Journal of Oral Biosciences　54　138-143　2012/01/01

    DOI： 10.1016/j.job.2012.02.005 　

    ISSN： 1349-0079

    More details Close

    Research approaches to biofilm are stratified by a series of analyses: (1) microbial number and species; (2) microbial proteins, such as enzymes; and (3) microbial activity, such as metabolic activity. On the other hand, the hierarchical structure of biology includes the genome, proteome, and metabolome, in which the metabolome is the final output of biological function. Metabolome analysis is the comprehensive analysis of the metabolome, a new strategy for biological research in the 21st century. The stratified structure of biofilm research corresponds to the biological hierarchy, and the analysis of microbial activity, especially metabolic activity, is comparable to metabolome analysis; however, oral biofilm samples are too small to analyze the metabolome by conventional methods. Recently, a new device involving capillary electrophoresis (CE) and time-of-flight mass spectrometry (MS) has been developed, facilitating metabolomic investigation of the central carbon metabolic pathways (i.e., the EMP pathway, pentose phosphate pathway, and TCA cycle) in oral biofilm. Using CE-MS, we analyzed metabolome profiles of oral biofilm after oral rinsing with glucose in vivo and evaluated the effects of oral rinsing with fluoride and xylitol on the metabolome profiles of oral biofilm. The results were somewhat consistent with previous in vitro data obtained from single bacterial strains, namely, Streptococcus and Actinomyces; however, new information describing the metabolic properties of oral biofilm was also obtained. This metabolomic approach will reveal the functional characteristics of oral biofilm in vivo, potentially providing new insights into the nature of oral biofilm in health and disease. ? 2012 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.

  75. Metabolomic Effects of Xylitol and Fluoride on Plaque Biofilm in Vivo

    N. Takahashi, J. Washio

    JOURNAL OF DENTAL RESEARCH　90　(12)　1463-1468　2011/12

    DOI： 10.1177/0022034511423395 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  76. Evaluation of pH at the Bacteria-Dental Cement Interface

    G. Mayanagi, K. Igarashi, J. Washio, K. Nakajo, H. Domon-Tawaraya, N. Takahashi

    JOURNAL OF DENTAL RESEARCH　90　(12)　1446-1450　2011/12

    DOI： 10.1177/0022034511423392 　

    ISSN： 0022-0345

    eISSN： 1544-0591

  77. Metabolomics of supragingival plaque and oral bacteria. Peer-reviewed

    Takahashi N, Washio J, Mayanagi G

    Journal of dental research　89　(12)　1383-1388　2010/12

    DOI： 10.1177/0022034510377792 　

    ISSN： 0022-0345

  78. Difference between age generation of oral health examination in a rural town Peer-reviewed

    Naoko Tanda, Kyoko Ikawa, Jumpei Washio, Yoshiko Shigihara, Yoshiro Shibuya, Masaki Iwakura, Megumi Naga, Yuhei Ogawa, Katsuhiko Taura, Takeyoshi Koseki

    INTERFACE ORAL HEALTH SCIENCE 2009　323-+　2010

    DOI： 10.1007/978-4-431-99644-6_94 　

  79. Short-time effect of fluoride on acid production by Streptococcus mutans Peer-reviewed

    Hitomi Domon, Kazuko Nakajo, Jumpei Washio, Harumi Miyasawa-Hori, Satoshi Fukumoto, Nobuhiro Takahashi

    INTERFACE ORAL HEALTH SCIENCE 2009　269-+　2010

    DOI： 10.1007/978-4-431-99644-6_74 　

  80. Hydrogen-sulfide production from various substrates by oral Veillonella and effects of lactate on the production Peer-reviewed

    Jumpei Washio, Yoko Sakuma, Yuko Shimada, Nobuhiro Takahashi

    INTERFACE ORAL HEALTH SCIENCE 2009　250-251　2010

    DOI： 10.1007/978-4-431-99644-6_66 　

  81. Challenge to metabolomics of oral biofilm -From "What are they?" to "What are they doing?"-

    Jumpei Washio, Gen Mayanagi, Nobuhiro Takahashi

    Journal of Oral Biosciences　52　(3)　225-232　2010/01/01

    Publisher: Japanese Association for Oral Biology

    DOI： 10.2330/joralbiosci.52.225 　

    ISSN： 1349-0079

    More details Close

    There has been extensive bacteriological research into oral biofilm. Most of these studies examined the bacterial composition of oral biofilm and the bacterial species specific to oral diseases. It is thought that the various metabolites, intracellular metabolic intermediates and end-products produced by metabolic activities of oral biofilm directly relate to oral diseases, such as dental caries and periodontitis. However, the metabolic properties, including functional metabolic pathways and their metabolic regulations, havenot been clarified sufficiently because the comprehensive identification and quantification of metabolites, that is, metabolomics - or metabolome analysis are technically difficult. Recently, a new device consisting of capillary electrophoresis(CE) and a tim-of-flight mass spectrometer (TOFMS) has been developed, facilitating the metabolomic investigation of sugar metabolism in oral biofilm. The results clearly showed that oral biofilm metabolomics is possible even with a very small sample, and that almost all metabolites in the central carbon metabolic pathways, the EMP pathway, the pentose-phosphate pathway, and the TCA cycle are present in the oral biofilm in vivo. Metabolomics using CE-TOFMS may offer a new frame of knowledge to answer the question &quot;What are bacteria doing in oralbiofilm?&quot; from a metabolic perspective, and possibly provide insight into the relationship between the metabolic properties of oral biofilm and the etiology of oral disease,as well as the influences of medicines, such as fluoride, or a sugar substitute, such as xylitol, on metabolism of the oral biofilm.

  82. New quantitative fluorometry for evaluating oral bacterial adhesion to biomaterials Peer-reviewed

    Sakuma Yoko, Washio Jumpei, Takeuchi Yasuhisa, Sasaki Keiichi, Takahashi Nobuhiro

    INTERFACE ORAL HEALTH SCIENCE 2009　215-+　2010

    DOI： 10.1007/978-4-431-99644-6_52 　

  83. Transient acid-impairment of growth ability of oral Streptococcus, Actinomyces, and Lactobacillus: a possible ecological determinant in dental plaque

    M. Horiuchi, J. Washio, H. Mayanagi, N. Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　24　(4)　319-324　2009/08

    DOI： 10.1111/j.1399-302X.2009.00517.x 　

    ISSN： 0902-0055

  84. Hydrogen sulfide production by oral Veillonella: effects of substrate and environmental pH Peer-reviewed

    J.Washio, S.Matoba, T.Seki, NYamamoto, M. Yamamoto, N. Takahashi

    Interface Oral Health Science2007　219-224　2008/02

  85. Inhibitory effects of maltotriitol on growth and adhesion of mutans streptococci Peer-reviewed

    Harumi Miyasawa-Hori, Shizuko Aizawa, Jumpei Washio, N. Takahashi

    Interface Oral Health Science　257　2008/02

  86. Dental examination for oral health promotion in a rural town Peer-reviewed

    Naoko Tanda, Masaki IwakuraKyoko, IkawaJumpei, WashioAyumi, Kusano Kazutaka Amano, Yuhei Ogawa, Yudai Yamada Yoshiko Shigihara Yoshiro, Shibuya Megumi, Haga Ken Osaka, Takeyoshi Koseki

    Interface Oral Health Science 2007　299　2008/02

  87. psychological characterization of halitosis patients by using Egogram and the Halitosis scale questionare Peer-reviewed

    Ayumi Kusano, Masaki Iwakura, Kyoko Ikawa, Naoko Tanda, Jumpei, Washio Yuhei Ogawa, Yudai Yamada, Takeyoshi Koseki

    Interface oral Health Science 2007　307　2008/02

  88. A new portable sulfide monitor with a zinc-oxide semiconductor sensor for daily use and field study International-journal Peer-reviewed

    Naoko Tanda, Jumpei Washio, Kyoko Ikawa, Kengo Suzuki, Takeyoshi Koseki, Masaki Iwakura

    JOURNAL OF DENTISTRY　35　(7)　552-557　2007/07

    DOI： 10.1016/j.jdent.2007.03.003 　

    ISSN： 0300-5712

  89. Relationship between hydrogen sulfide-producing bacteria of the tongue coating and oral malodor Peer-reviewed

    J. Washio, T. Sato, K. Ikawa, N. Tanda, M. Iwakura, T. Koseki, N. Takahashi

    International Congress Series　1284　199-200　2005/09

    DOI： 10.1016/j.ics.2005.06.068 　

    ISSN： 0531-5131

  90. Circadian changes of volatile sulfur compounds measured by Breathtron Peer-reviewed

    k.Ikawa, M.Iwakura, J.Washio, A, Kusano, N, Tanda, T.Koseki

    International Congress Series　1284　89-90　2005/07

    DOI： 10.1016/j.ics.2005.06.008 　

  91. Development of a portable bad-breath monitor and application to field study of halitosis Peer-reviewed

    N .Tanda, M, Iwakura, K.Ikawa, Washio, A, Kusano K.Suzuki, T. Koseki

    International Congress Series　1284　201-202　2005/07

    DOI： 10.1016/j.ics.2005.06.064 　

  92. Hydrogen sulfide-producing bacteria in tongue coating and their relationship with oral malodor International-journal Peer-reviewed

    Washio J, Sato T, Koseki T, Takahashi N

    Journal of Medical Microbiology　54　(9)　889-895　2005/04

    DOI： 10.1099/jmm.0.46118-0 　

    ISSN： 0022-2615

    More details Close

    The aims of this study were to identify hydrogen sulfide (H2S)-producing bacteria among tongue biofilm microflora and to investigate the relationship between bacterial flora and H2S levels in mouth air. Oral malodour levels in 10 subjects (age 21-56 years) were assessed by gas chromatography, and Breathtron and organoleptic scores. Based on these assessments, subjects were divided into two groups: an odour group and a no/low odour group. Tongue coatings were sampled and spread onto Fastidious Anaerobe Agar plates containing 0.05% cysteine, 0.12% glutathione and 0.02% lead acetate, and were then incubated anaerobically at 37 degrees C for 2 weeks. Bacteria forming black or grey colonies were selected as H2S-producing phenotypes. The numbers of total bacteria (P<0.005) and H2S-producing bacteria (P<0.05) in the odour group were significantly larger than those in the no/low odour group. Bacteria forming black or grey colonies (126 isolates from the odour group; 242 isolates from the no/low odour group) were subcultured, confirmed as producing H2S and identified according to 16S rRNA gene sequencing. Species of Veillonella (38.1% in odour group; 46.3% in no/low odour group), Actinomyces (25.4%; 17.7%) and Prevotella (10.3%; 7.8%) were the predominant H2S-producing bacteria in both the odour and no/low odour groups. These results suggest that an increase in the number of H2S-producing bacteria in the tongue biofilm is responsible for oral malodour, although the bacterial composition of tongue biofilm was similar between the two groups.

  93. Identification of mutans streptococci by restriction fragment length polymorphism analysis of polymerase chain reaction-amplified 16S ribosomal RNA genes Peer-reviewed

    T Sato, JP Hu, K Ohki, M Yamaura, J Washio, J Matsuyama, N Takahashi

    ORAL MICROBIOLOGY AND IMMUNOLOGY　18　(5)　323-326　2003/10

    DOI： 10.1034/j.1399-302X.2003.00095.x 　

    ISSN： 0902-0055

  94. Nested PCR for detection of mutans streptococci in dental plaque Peer-reviewed

    T Sato, J Matsuyama, T Kumagai, G Mayanagi, M Yamaura, J Washio, N Takahashi

    LETTERS IN APPLIED MICROBIOLOGY　37　(1)　66-69　2003

    DOI： 10.1046/j.1472-765X.2003.01359.x 　

    ISSN： 0266-8254

Show all ︎Show first 5

Misc. 119

  1. Nitrite Production from Nitrate in the Oral Microbiome and Its Contribution to Oral and Systemic Health. International-journal

    Jumpei Washio, Nobuhiro Takahashi

    Advances in experimental medicine and biology　1472　89-101　2025

    DOI： 10.1007/978-3-031-79146-8_6 　

    ISSN： 0065-2598

  2. 緑茶カテキンEGCGによる歯周病関連細菌に対する抗菌効果,およびう蝕関連細菌に対する効果との比較

    樋口 真由, 安彦 友希, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　74　(増刊)　113-113　2024/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  3. Development of micro-device to measure metabolic-related markers with ultra-high sensitivity

    鷲尾純平, 高橋信博, 安彦友希, 米田将太, 柳田保子

    生体医歯工学共同研究拠点成果報告書　2023　2024

  4. 口腔Actinomyces属及びSchaalia属の亜硝酸塩産生活性と活性に対する環境要因の影響

    大竹 知菜, 鷲尾 純平, 安彦 友希, 五十嵐 薫, 高橋 信博

    Journal of Oral Biosciences Supplement　2023　[P2-31]　2023/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  5. 歯周病関連細菌に由来する酪酸が歯周組織細胞機能に与える影響

    大谷 栄毅, 鷲尾 純平, 佐藤 聡子, 佐々木 詩織, 山田 聡, 高橋 信博

    Journal of Oral Biosciences Supplement　2023　[P1-07]　2023/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  6. 静脈麻酔薬プロポフォールが肝細胞の糖代謝活性に及ぼす影響

    佐々木 詩織, 鷲尾 純平, 大谷 栄毅, 佐藤 聡子, 水田 健太郎, 高橋 信博

    Journal of Oral Biosciences Supplement　2023　[P1-10]　2023/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  7. 口腔マイクロバイオームは発がん性物質アセトアルデヒドの分解にも関与する アセトアルデヒド産生能・分解能の簡易スクリーニング法の確立

    佐藤 知佳, 互野 亮, 鷲尾 純平, 安彦 友希, 五十嵐 薫, 高橋 信博

    Journal of Oral Biosciences Supplement　2023　[P1-27]　2023/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  8. 歯科訪問診療における水質管理方策の検討

    伏見 国拡, 山田 将博, 渡辺 隼, 鷲尾 純平, 高橋 信博, 江草 宏

    東北大学歯学雑誌　41/42　(2/1)　45-45　2023/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  9. ペットボトル飲料物の保管に関する食品科学的・細菌学的研究

    河内 美帆, 涌井 杏奈, 賀来 ながら, 高橋 七瀬, 宮沢 美里, 佐藤 彩, 阿部 峰士, 今井 真奈美, 佐藤 遥菜, 加藤 優希, 岡部 璃佳, 成瀬 悠香, 佐藤 奈緒, 鷲尾 純平, 佐藤 拓一

    口腔衛生学会雑誌　73　(増刊)　153-153　2023/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  10. 緑茶カテキンの歯周病関連細菌に対する抗菌作用 アミノ酸代謝活性抑制および菌体凝集作用について

    樋口 真由, 安彦 友希, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　73　(増刊)　197-197　2023/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  11. 唾液組成のモニタリングに向けた全固体型イオン選択電極のマイクロ流路への統合

    真柳 弦, 鷲尾 純平, 高橋 信博, 大関 祥久, 山田 哲也, 石原 昇, 柳田 保子

    生体医歯工学共同研究拠点成果報告書　2022年度　158-158　2023/04

    Publisher: 東京医科歯科大学生体材料工学研究所

  12. 歯周病関連細菌に由来する酪酸が歯周組織細胞機能に与える影響

    大谷栄毅, 大谷栄毅, 鷲尾純平, 佐藤聡子, 佐々木詩織, 山田聡, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2023　2023

    ISSN： 2187-9109

  13. 細菌性代謝産物の酪酸が歯肉線維芽細胞の機能に与える影響

    大谷栄毅, 大谷栄毅, 鷲尾純平, 國富葵, 佐々木詩織, 大橋一正, 山田聡, 高橋信博

    日本歯周病学会会誌(Web)　65　2023

    ISSN： 1880-408X

  14. 口腔マイクロバイオームは発がん性物質アセトアルデヒドの分解にも関与する。-アセトアルデヒド産生能・分解能の簡易スクリーニング法の確立-

    佐藤知佳, 佐藤知佳, 互野亮, 互野亮, 鷲尾純平, 安彦友希, 五十嵐薫, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2023　2023

    ISSN： 2187-9109

  15. 口腔Actinomyces属及びSchaalia属の亜硝酸塩産生活性と活性に対する環境要因の影響

    大竹知菜, 大竹知菜, 鷲尾純平, 安彦友希, 五十嵐薫, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2023　2023

    ISSN： 2187-9109

  16. Scardovia wiggsiaeの増殖促進因子の検討(Growth promoting factors of Scardovia wiggsiae)

    亀田 真衣, 安彦 友希, 鷲尾 純平, 佐藤 聡子, 高橋 信博

    Journal of Oral Biosciences Supplement　2022　260-260　2022/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  17. Growth promoting factors of Scardovia wiggsiae

    亀田真衣, 亀田真衣, 安彦友希, 鷲尾純平, 佐藤聡子, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2022　2022

    ISSN： 2187-9109

  18. Integration of all-solid-state electrode into the microfluidic channel

    大関祥久, 山田哲也, 石原昇, 柳田保子, 真柳弦, 真柳弦, 鷲尾純平, 高橋信博

    精密工学会大会学術講演会講演論文集　2022　2022

  19. 集団微生物学と細菌バイオフィルム研究の前線 口腔と全身の健康に関わる口腔バイオフィルムの代謝機能(Metabolic function of oral biofilm involved in oral and systemic health)

    高橋 信博, 鷲尾 純平

    日本細菌学雑誌　76　(1)　31-31　2021/02

    Publisher: 日本細菌学会

    ISSN： 0021-4930

    eISSN： 1882-4110

  20. The Nitrite producing activity of oral Neisseria and the effect of environmental factors

    鷲尾純平, 江副和子, 佐藤聡子, 安彦友希, 小峰英也, 柴田怜, 田花航平, MAO X, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2021　2021

    ISSN： 2187-9109

  21. Study of all-solid-state ion selective electrodes for multi-ion monitoring in the intraoral

    真柳弦, 鷲尾純平, 高橋信博, 大久保信吾, 山田哲也, 石原昇, 柳田保子

    生体医歯工学共同研究拠点成果報告書　2020　96-96　2021

    Publisher: 東京医科歯科大学生体材料工学研究所

  22. 飲酒を想定した中から高濃度エタノール環境下におけるStreptococcus属によるアセトアルデヒド産生

    互野 亮, 鷲尾 純平, 佐々木 啓一, 高橋 信博

    Journal of Oral Biosciences Supplement　2020　162-162　2020/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  23. 口腔内細菌叢解析システム PCR-RFLP法によるプロファイリング

    河内 美帆, 丸山 伸吾, 枡田 菜々子, 佐野 拓人, 涌井 杏奈, 八巻 惠子, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    Journal of Oral Biosciences Supplement　2020　393-393　2020/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  24. Fabrication of calcium ion selective electrode for intraoral multi ion measurement microdevice

    齊藤滉佑, 石原昇, 柳田保子, 真柳弦, 鷲尾純平, 高橋信博

    応用物理学会春季学術講演会講演予稿集(CD-ROM)　67th　2020

  25. 緑茶カテキンEGCGはStreptococcus mutansの酸産生を抑制し菌体凝集を促進する(Green tea-derived epigallocatechin gallate inhibited the acid production of Streptococcus mutans and promoted the bacterial aggregation)

    Sili Han, 安彦 友希, 鷲尾 純平, Yufang Luo, Linglin Zhang, 高橋 信博

    Journal of Oral Biosciences Supplement　2019　142-142　2019/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  26. 温熱による癌細胞の糖代謝活性への影響

    小林 ゆり, 鷲尾 純平, 篠原 優太, 高橋 信博

    Journal of Oral Biosciences Supplement　2019　199-199　2019/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  27. PCR-RFLP法を用いた口腔内細菌叢解析(プロファイリング)システム

    佐野 拓人, 涌井 杏奈, 八巻 惠子, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    Journal of Oral Biosciences Supplement　2019　256-256　2019/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  28. フッ化物による歯周病関連菌Porphyromonas gingivalisの増殖・代謝の抑制効果

    土門 ひと美, 鷲尾 純平, 安彦 友希, 川嶋 順子, 高橋 信博

    Journal of Oral Biosciences Supplement　2019　281-281　2019/10

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  29. 《研究報告書》液体ミルクの安全性の立証に向けて：飲み残したペットボトルの口の部分に付着する細菌および飲料物内の細菌の量および構成の解析 Invited

    佐藤 拓一, 鷲尾 純平

    総合健康推進財団 第34回 一般研究奨励助成事業による研究報告書　49-56　2019/08

    Publisher: 総合健康推進財団

  30. プラークのもつ硝酸還元活性・亜硝酸分解活性と酸産生活性抑制効果との関連

    山本 祐慈, 清水 弘一, 五十嵐 公英, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　69　(増刊)　168-168　2019/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  31. フッ化物による歯周病関連菌Porphyromonas gingivalisの増殖・代謝の抑制効果

    土門ひと美, 鷲尾純平, 安彦友希, 川嶋順子, 高橋信博

    Journal of Oral Biosciences Supplement (Web)　2019　2019

    ISSN： 2187-9109

  32. 【歯科医療スタッフにおける感染予防対策の実際-歯科医師、歯科技工士、歯科衛生士等の歯科医療従事者連携による院内感染対策-】歯科用ユニットの水質管理 "フラッシング"の重要性

    山田 将博, 鷲尾 純平, 高橋 信博, 江草 宏

    日本歯科理工学会誌　37　(4)　223-226　2018/11

    Publisher: (一社)日本歯科理工学会

    ISSN： 1884-4421

  33. 口腔細菌は弱アルカリ性好気環境で高いアセトアルデヒド産生を示す

    互野 亮, 鷲尾 純平, 丹田 奈緒子, 佐々木 啓一, 高橋 信博

    Journal of Oral Biosciences Supplement　2018　170-170　2018/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  34. 環境中の硝酸塩による口腔Veillonella属の亜硝酸産生能の増加(Environmental nitrate increased the nitrite production of oral Veillonella)

    Dimas Prasetianto Wicaksono, 鷲尾 純平, 高橋 信博

    Journal of Oral Biosciences Supplement　2018　300-300　2018/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  35. 口腔細菌は弱アルカリ性好気環境で高いアセトアルデヒド産生を示す

    互野 亮, 鷲尾 純平, 丹田 奈緒子, 佐々木 啓一, 高橋 信博

    Journal of Oral Biosciences Supplement　2018　307-307　2018/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  36. ニプルを通して飲んだ際の、ベビー飲料内への口腔細菌の流入

    相原 瞳, 涌井 杏奈, 佐野 拓人, 河内 美帆, 鷲尾 純平, 安彦 友希, 真柳 弦, 八巻 恵子, 高橋 信博, 佐藤 拓一

    Journal of Oral Biosciences Supplement　2018　353-353　2018/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  37. プラークのもつ硝酸還元活性と亜硝酸還元活性

    山本 祐慈, 清水 弘一, 五十嵐 公英, 鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　68　(増刊)　178-178　2018/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  38. 細菌-材料インターフェイスにおけるフッ素徐放性材料のpH低下抑制効果の評価および将来への展望

    真柳 弦, 鷲尾 純平, 高橋 信博, 柳田 保子, 伊藤 浩之, 石原 昇, 益 一哉

    生体医歯工学共同研究拠点成果報告書　平成29年度　98-98　2018/04

    Publisher: 東京医科歯科大学生体材料工学研究所

  39. ニプルを通して飲んだ際の,ベビー飲料内への口腔細菌の流入

    相原瞳, 涌井杏奈, 佐野拓人, 河内美帆, 鷲尾純平, 安彦友希, 真柳弦, 八巻恵子, 高橋信博, 佐藤拓一

    Journal of Oral Biosciences Supplement (Web)　2018　2018

    ISSN： 2187-9109

  40. 乳酸濃度およびpHの変動がVeillonella atypicaの亜硝酸産生能に及ぼす影響(Effect of lactate and pH on nitrite producing activity of Veillonella atypica)

    Wicaksono Dimas Prasetianto, 鷲尾 純平, 高橋 信博

    Journal of Oral Biosciences Supplement　2017　354-354　2017/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  41. 飲料物の飲み口に付着する細菌の量および構成の解析

    佐野 拓人, 平吹 有香, 涌井 杏奈, 曾田 彩花, 安彦 友希, 八巻 恵子, 真柳 弦, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    Journal of Oral Biosciences Supplement　2017　399-399　2017/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  42. 口腔バイオフィルム細菌の増殖・代謝抑制作用を有する天然植物抽出物の探索

    北郡 秀晃, 鷲尾 純平, 互野 亮, 高橋 信博

    Journal of Oral Biosciences Supplement　2017　502-502　2017/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  43. 飲料物の飲み口に付着する細菌の量および構成の解析

    佐野拓人, 平吹有香, 涌井杏奈, 曾田彩花, 安彦友希, 八巻恵子, 真柳弦, 鷲尾純平, 高橋信博, 佐藤拓一

    Journal of Oral Biosciences Supplement (Web)　2017　2017

    ISSN： 2187-9109

  44. 代謝モニタリング・システムを用いた癌細胞の糖・アミノ酸代謝活性測定と代謝活性に対する環境pHの影響

    森島 浩允, 鷲尾 純平, 北村 淳, 高橋 哲, 高橋 信博

    Journal of Oral Biosciences Supplement　2016　296-296　2016/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  45. 長期使用義歯からの細菌由来揮発性代謝産物の検出

    互野 亮, 末永 華子, 鷲尾 純平, 佐々木 啓一, 高橋 信博

    Journal of Oral Biosciences Supplement　2016　298-298　2016/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  46. 口腔扁平上皮癌のグルコース代謝に対するジクロロ酢酸ナトリウム及び2-デオキシ-D-グルコースの抑制効果

    北村 淳, 鷲尾 純平, 森島 浩允, 高橋 哲, 高橋 信博

    Journal of Oral Biosciences Supplement　2016　307-307　2016/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  47. 蛍光色素を用いた口腔プラークバイオフィルム代謝活性の高感度測定法

    鷲尾 純平, 石黒 和子, 入江 大貴, 内山 愛理, 高橋 信博

    Journal of Oral Biosciences Supplement　2016　558-558　2016/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  48. プロポリスエタノール抽出物が誘導するGr-1+、CD11b+細胞の評価

    鷲尾 浩平, 藤本 純平, 北村 浩

    日本獣医学会学術集会講演要旨集　159回　508-508　2016/08

    Publisher: (公社)日本獣医学会

    ISSN： 1347-8621

  49. 蛍光色素を用いた細菌の代謝活性リアルタイム・モニター法の確立

    石黒 和子, 鷲尾 純平, 佐々木 啓一, 高橋 信博

    Journal of Oral Biosciences Supplement　2014　152-152　2014/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  50. 亜硝酸塩のStreptococcus mutans酸産生抑制機構をメタボロミクスで解明する

    鷲尾 純平, 山本 祐慈, 高橋 信博

    Journal of Oral Biosciences Supplement　2014　219-219　2014/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  51. CAD/CAM総義歯用アクリルレジンへのS.mutansの付着に関する検討

    折居 雄介, 竹内 裕尚, 道井 貴幸, 福島 庄一, 石黒 和子, 鷲尾 純平, 阿部 二郎, 熊谷 知弘, 高橋 信博, 佐々木 啓一

    日本補綴歯科学会誌　6　(特別号)　193-193　2014/05

    Publisher: (公社)日本補綴歯科学会

    ISSN： 1883-4426

    eISSN： 1883-6860

  52. 母体の歯周病罹患が胎児口蓋裂発症に与える影響の分子生物学的探索

    菅崎 弘幸, 鷲尾 純平, Kawai Toshihisa, Nichols Frank C, 五十嵐 薫

    日本口蓋裂学会雑誌　39　(2)　96-96　2014/04

    Publisher: (一社)日本口蓋裂学会

    ISSN： 0386-5185

    eISSN： 2186-5701

  53. メタボローム解析による口腔扁平上皮癌のエネルギー代謝特性の解明とバイオマーカーの探索

    小川 珠生, 鷲尾 純平, 高橋 哲, 高橋 信博

    Journal of Oral Biosciences Supplement　2013　158-158　2013/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  54. 歯周病関連菌の義歯用レジン(PMMA)表面への付着性および唾液による影響

    石黒 和子, 鷲尾 純平, 佐久間 陽子, 竹内 裕尚, 福島 庄一, 佐々木 啓一, 高橋 信博

    Journal of Oral Biosciences Supplement　2013　160-160　2013/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  55. ヒト・プラークの糖アルコール代謝 メタボロミクス的アプローチ

    鷲尾 純平, 高橋 信博

    Journal of Oral Biosciences Supplement　2013　221-221　2013/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  56. Divalent cations enhance fluoride binding to Streptococcus mutans and Streptococcus sanguinis cells and subsequently inhibit bacterial acid production

    H. Domon-Tawaraya, H. Domon-Tawaraya, K. Nakajo, J. Washio, T. Ashizawa, T. Ichino, H. Sugawara, S. Fukumoto, N. Takahashi

    Caries Research　47　(2)　141-149　2013/01/01

    DOI： 10.1159/000344014 　

    ISSN： 0008-6568

  57. 蛍光色素alamar Blueを用いたバイオマテリアル付着歯周病関連細菌定量法

    石黒 和子, 鷲尾 純平, 佐久間 陽子, 竹内 裕尚, 佐々木 啓一, 高橋 信博

    Journal of Oral Biosciences Supplement　2012　126-126　2012/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  58. Dental plaque biofilm中のアミノ酸の代謝機構 CE-TOFMSを用いたメタボロミクス的視点から

    鷲尾 純平, 高橋 信博

    Journal of Oral Biosciences Supplement　2012　141-141　2012/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 2187-2333

    eISSN： 2187-9109

  59. メタボローム解析で見えてきた口腔バイオフィルムの機能 「何がいるか」から「何をしているか」へ

    鷲尾 純平, 眞柳 弦, 小川 珠生, 高橋 信博

    日本歯科医師会雑誌　65　(5)　616-616　2012/08

    Publisher: (公社)日本歯科医師会

    ISSN： 0047-1763

  60. プラークと唾液のメタボローム解析 口腔内でのアミノ酸の利用に注目して

    鈴木 啓佑, 月星 陽介, 渡邊 基博, 小川 珠生, 鷲尾 純平, 高橋 信博

    日本歯科医師会雑誌　65　(5)　686-686　2012/08

    Publisher: (公社)日本歯科医師会

    ISSN： 0047-1763

  61. Bicarbonate decreases inhibitory effects of fluoride on acid production by Actinomyces naeslundii

    Junko Kawashima, Kazuko Nakajo, Jumpei Washio, Hidetoshi Shimauchi, Nobuhiro Takahashi

    Interface Oral Health Science 2011　189-191　2012/01/01

    DOI： 10.1007/978-4-431-54070-0_51 　

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    ? Springer 2012. This study aimed to evaluate the effects of bicarbonate and fluoride on the acid production ability of Actinomyces naeslundii. In the presence of bicarbonate, the inhibitory effect of fluoride was decreased, suggesting that Actinomyces species have a unique mechanism to escape or weaken the inhibitory effect of fluoride. This might be relevant to that Actinomyces species has a bicarbonate-assimilating pathway for succinate production in addition to the glycolytic pathway.

  62. A new method to evaluate pH at the interface between parasites and biomaterials

    Gen Mayanagi, Koei Igarashi, Jumpei Washio, Kazuko Nakajo, Hitomi Domon-Tawaraya, Nobuhiro Takahashi

    Interface Oral Health Science 2011　192-194　2012/01/01

    DOI： 10.1007/978-4-431-54070-0_52 　

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    ? Springer 2012. Physiochemical assessment of the parasite-biomaterial interface is essential to develop new biomaterials. The purpose of this study was to develop a method to evaluate pH at the parasite-biomaterial interface and demonstrate physiochemical interaction at the interface. An experimental apparatus with a well for pH monitoring was made of acrylic resin with a bottom of test materials. The well was filled with Streptococcus mutans NCTC 10449, and an ion-sensitive field-effect transistor (ISFET) pH electrode was placed at an interface between bacterial cells and materials. The pH was monitored after the addition of 1% glucose at 37°C. The pH at the parasite-biomaterial interface was successfully monitored using the experimental apparatus with an ISFET pH electrode. This method could be useful to assess the pH at the parasite-biomaterial interface.

  63. Metabolomics of oral plaque biofilm using CE-TOFMS

    Jumpei Washio, Gen Mayanagi, Nobuhiro Takahashi

    Interface Oral Health Science 2011　218-220　2012/01/01

    DOI： 10.1007/978-4-431-54070-0_61 　

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    ? Springer 2012. The various metabolites produced by oral plaque biofilm directly relate to oral diseases such as dental caries and periodontitis. However, the metabolic properties in oral plaque biofilm have not been clarified because the comprehensive identification and quantification of metabolites, metabolomics in short, was technically difficult. Recently, CE-TOFMS, a combination of capillary electrophoresis and time-of-flight mass spectrometry, has been developed to perform metabolomics, and therefore, we applied CE-TOFMS to metabolomics of oral plaque biofilm. Consequently, it was suggested that it is possible to detect and quantify metabolites related to the Embden-Meyerhof-Parnas pathway, the pentose-phosphate pathway and the citricacid cycle, the metabolites related to amino acid metabolisms and amino acids in a small amount of oral plaque biofilm, and that these metabolic pathways function in oral plaque biofilm. The metabolomics research using CE-TOFMS may contribute to the clarification of the etiology of oral plaque biofilm-related diseases.

  64. A highly sensitive alamarBlue? method for evaluating bacterial adhesion to biomaterials

    Yoko Sakuma, Jumpei Washio, Yasuhisa Takeuchi, Keiichi Sasaki, Nobuhiro Takahashi

    Interface Oral Health Science 2011　201-203　2012/01/01

    DOI： 10.1007/978-4-431-54070-0-55 　

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    ? Springer 2012. It is known that biofilm formed on the denture surface (denture plaque) causes various oral diseases and the aspiration pneumonia. For prevention of these diseases, new denture materials that inhibit or reduce the formation of denture plaque are strongly required. In order to develop these materials, it is essential to evaluate the bacterial adhesion to material surfaces. Thus, we established a new non-radioisotopic method for evaluating the adhesion of bacteria, using. alamar- Blue?, a fluorescent reduction-oxidation indicator, that responds quantitatively to the bacterial metabolic activity. Furthermore, we enhanced the sensitivity of the method by adding bacterial metabolic substrates to the reaction mixture. It is suggested that the simple and precise quantification of bacterial adhesion to material surfaces becomes possible by this improved alamarBlue? method.

  65. Breath acetone in type 1 and type 2 diabetes mellitus

    Naoko Tanda, Yoshinori Hinokio, Jumpei Washio, Nobuhiro Takahashi, Takeyoshi Koseki

    Interface Oral Health Science 2011　212-214　2012/01/01

    DOI： 10.1007/978-4-431-54070-0_59 　

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    ? Springer 2012. Difference of breath acetone between type 1 and type 2 diabetes mellitus (DM) is examined under informed consent. We measure acetone in mouth air and in deep breath by Gas Analyzer? XG-100V (New Cosmos Electric Co., Ltd., Osaka, Japan) and volatile sulfide compound in mouth air by Breathtron? (New Cosmos Electric Co., Ltd., Osaka, Japan), after meal. The acetone ratio of deep exhaled breath to mouth air and concentration of volatile sulfide compound (VSC) are com- pared between type 1 and type 2 DM patients. The acetone ratio of deep exhaled breath to mouth air is significantly higher in type 1 DM than type 2 DM patients. The concentration of VSC shows no significant difference. Though more cases should be studied, there is a possibility that the acetone ratio might reflect metabolic disorder of type 1 DM patients.

  66. Metabolomic approach to oral microbiota

    Nobuhiro Takahashi, Jumpei Washio, Gen Mayanagi

    Interface Oral Health Science 2011　334-340　2012/01/01

    DOI： 10.1007/978-4-431-54070-0_98 　

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    ? Springer 2012. Oral microbiota, known as dental plaque or oral biofilm, is a complex of microorganisms. Functions such as metabolic activity are crucial final outputs of microbiota, since they directly relate to the pathogenicity of oral diseases, such as dental caries and periodontitis. The metabolic characteristics of microbiota have been speculated from the results of in vitro studies of representative bacteria consisting of microbiota, because the amount of oral microbiota sampled from the oral cavity was too small for metabolic study. A new system of metabolome anal ysis (metabolomics), using the combination of capillary electrophoresis and a time of flight mass spectrometer, has been developed, enabling us to quantify hundreds of intracellular metabolic intermediates relevant to the central carbon metabolism in supragingival plaque. The metabolomic approach may offer new insights into understanding the metabolic activity of microbiota, as “a supra organism” consisting of a tremendous number of organisms that may be considered a single organism.

  67. Divalent cations enhance short-time fluoride exposure-induced inhibition on acid production by Streptococcus mutans

    Hitomi Domon-Tawaraya, Hitomi Domon-Tawaraya, Kazuko Nakajo, Jumpei Washio, Satoshi Fukumoto, Nobuhiro Takahashi

    Interface Oral Health Science 2011　178-180　2012/01/01

    DOI： 10.1007/978-4-431-54070-0_47 　

  68. ヒト・プラークの糖代謝に対するフッ化物およびキシリトールの影響 CE-TOFMSを用いたメタボロミクス・アプローチ

    鷲尾 純平, 高橋 信博

    Journal of Oral Biosciences　53　(Suppl.)　139-139　2011/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  69. ヒト・プラークとう蝕関連口腔細菌Streptococcus mutansの糖代謝に対するキシリトールの影響の相違

    鷲尾 純平, 高橋 信博

    日本生化学会大会プログラム・講演要旨集　84回　4P-0453　2011/09

    Publisher: (公社)日本生化学会

  70. 口腔フローラのプロファイリング

    佐藤 拓一, 安彦 友希, 長谷川 彩子, 真柳 弦, 鷲尾 純平, 中條 和子, 堀 はるみ, 宮澤, 高橋 信博

    東北大学歯学雑誌　30　(1)　33-33　2011/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  71. 根面齲触関連菌Actinomycesの酸産生活性とそれに及ぼす環境pH、重炭酸、フッ化物の影響

    川嶋 順子, 中條 和子, 鷲尾 純平, 島内 英俊, 高橋 信博

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集　134回　33-33　2011/05

    Publisher: (NPO)日本歯科保存学会

  72. 口腔環境を考慮した糖負荷試験時呼気中アセトン測定と血中ケトン体との関連

    丹田 奈緒子, 檜尾 好徳, 由浪 有希子, 鷲尾 純平, 高橋 信博, 小関 健由

    糖尿病　54　(Suppl.1)　S-216　2011/04

    Publisher: (一社)日本糖尿病学会

    ISSN： 0021-437X

    eISSN： 1881-588X

  73. CE-TOFMSを用いた口腔プラークバイオフィルムの糖代謝メタボローム解析~フッ化物やキシリトールによる影響をin vivoで探る~

    鷲尾純平, 小川珠生, 真柳弦, 高橋信博

    キャピラリー電気泳動シンポジウム講演要旨集　31st　2011

  74. イオン感受性電界効果型トランジスタ微小pH電極による細菌-歯科修復材料・インターフェイスpH測定

    真柳弦, 五十嵐公英, 鷲尾純平, 中條和子, 土門(俵谷)ひと美, 高橋信博

    粉体粉末冶金協会講演概要集　2011　2011

  75. 短時間フッ化物曝露によるStreptococcus mutans酸産生抑制効果に与える二価金属イオンの影響

    土門 ひと美, 俵谷, 中條 和子, 鷲尾 純平, 福本 敏, 高橋 信博

    Journal of Oral Biosciences　52　(Suppl)　107-107　2010/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  76. プラークバイオフィルムのメタボローム解析 糖代謝からアミノ酸代謝まで

    鷲尾 純平, 真柳 弦, 高橋 信博

    Journal of Oral Biosciences　52　(Suppl)　135-135　2010/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  77. 入院中に口腔環境改善を試みた治療コンプライアンスの低い2型糖尿病の1例

    丹田 奈緒子, 檜尾 好徳, 由浪 有希子, 鷲尾 純平, 高橋 信博, 小関 健由, 片桐 秀樹, 岡 芳知

    糖尿病　53　(7)　528-528　2010/07

    Publisher: (一社)日本糖尿病学会

    ISSN： 0021-437X

    eISSN： 1881-588X

  78. う蝕関連口腔細菌の糖代謝メタボローム解析 糖代謝機能の類似と相違

    鷲尾 純平, 真柳 弦, 高橋 信博

    日本生化学会大会プログラム・講演要旨集　82回　4T15a-2　2009/09

    Publisher: (公社)日本生化学会

  79. CE-TOFMSを用いたStreptococcus mutansおよびActinomyces naeslundiiの糖代謝メタボローム解析

    鷲尾 純平, 真柳 弦, 高橋 信博

    Journal of Oral Biosciences　51　(Suppl.)　104-104　2009/08

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  80. Actinomycesの糖代謝 フッ化物耐性とその重炭酸による影響

    川嶋 順子, 中條 和子, 鷲尾 純平, 島内 英俊, 高橋 信博

    Journal of Oral Biosciences　51　(Suppl.)　105-105　2009/08

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  81. 短時間フッ化物曝露によるStreptococcus mutansのフッ素吸着及び酸産生能への影響

    土門 ひと美, 俵谷, 中條 和子, 鷲尾 純平, 宮澤 はるみ, 堀, 福本 敏, 高橋 信博

    Journal of Oral Biosciences　51　(Suppl.)　113-113　2009/08

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  82. CE-TOFMSを用いたプラークバイオフィルムの糖代謝メタボローム解析

    鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　59　(4)　370-370　2009/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  83. 某高等専門学校男子学生における1975年から2005年のう蝕有病状況の改善について

    田浦 勝彦, 山田 雄大, 鈴木 淳, 伊藤 恵美, 井川 恭子, 丹田 奈緒子, 鷲尾 純平, 小関 健由

    東北大学歯学雑誌　28　(1)　13-17　2009/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  84. Effects of α-amylase and its inhibitors on acid production from cooked starch by oral streptococci

    S. Aizawa, S. Aizawa, H. Miyasawa-Hori, K. Nakajo, J. Washio, H. Mayanagi, S. Fukumoto, N. Takahashi, N. Takahashi

    Caries Research　43　(1)　17-24　2009/03/01

    DOI： 10.1159/000189703 　

    ISSN： 0008-6568

  85. バイオマテリアル表面への各種口腔細菌付着量の評価法

    佐久間 陽子, 鷲尾 純平, 竹内 裕尚, 佐々木 啓一, 高橋 信博

    Journal of Oral Biosciences　50　(Suppl.)　153-153　2008/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  86. 口腔Veillonellaはcysteine含有ペプチドから硫化水素を産生し、乳酸はその硫化水素産生を促進する

    鷲尾 純平, 佐久間 陽子, 島田 裕子, 高橋 信博

    Journal of Oral Biosciences　50　(Suppl.)　214-214　2008/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  87. 某高等専門学校男子学生の齲蝕の減少について 15歳と19歳の1975年から2005年までの成績から

    山田 雄大, 小川 裕平, 天野 一宇, 丹田 奈緒子, 井川 恭子, 田浦 勝彦, 鷲尾 純平, 小関 健由

    東北大学歯学雑誌　26　(2)　43-44　2007/12

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  88. 口腔Veillonellaはcystathionine β-synthase様活性を持ちシステインから硫化水素を産生する

    鷲尾 純平, 高橋 信博

    Journal of Oral Biosciences　49　(Suppl.)　161-161　2007/08

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  89. 口腔環境中の乳酸はVeillonellaの硫化水素産生を促進する

    鷲尾 純平, 高橋 信博

    口腔衛生学会雑誌　57　(4)　389-389　2007/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  90. 某高等専門学校男子学生の齲蝕有病状況について 15歳と19歳の1975年から2005年までの変化

    田浦 勝彦, 井川 恭子, 山田 雄大, 小川 裕平, 天野 一宇, 丹田 奈緒子, 鷲尾 純平, 小関 健由

    口腔衛生学会雑誌　57　(3)　228-228　2007/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  91. 町の一般健診に併設して導入に口臭を用いた学習歯科健診

    蒲生 正若, 蒲生 裕子, 木村 篤, 木村 歩, 吉田 厚子, 玉根 幸恵, 松本 百合恵, 高原 カネ子, 井川 恭子, 丹田 奈緒子, 草野 歩, 山田 雄大, 小川 裕平, 天野 一宇, 鴫原 ヨシ子, 伊藤 恵美, 鷲尾 純平, 渋谷 芳郎, 岩倉 政城, 小関 健由

    口腔衛生学会雑誌　57　(3)　228-229　2007/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  92. 津山町で実施した学習歯科健診の試み

    丹田 奈緒子, 岩倉 政城, 井川 恭子, 天野 一宇, 小川 裕平, 山田 雄大, 草野 歩, 鷲尾 純平, 鴫原 ヨシ子, 渋谷 芳郎, 小関 健由, 阿部 陽子, 小橋 純子, 渋谷 香奈, 小泉 香織, 成澤 喜美子, 芳賀 めぐみ, 小坂 健

    宮城県公衆衛生学会会誌　(39)　12-12　2007/03

    Publisher: 宮城県公衆衛生学会

    ISSN： 0912-747X

  93. 口腔Veillonellaによる硫化水素産生に関する検討 菌種・基質・環境pHによる違い

    鷲尾 純平, 高橋 信博

    Journal of Oral Biosciences　48　(Suppl.)　200-200　2006/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  94. 唾液細菌および口腔Veillonellaによる口臭成分の一つ硫化水素の産生とその唾液や口腔環境との関わり

    鷲尾 純平, 佐藤 拓一, 竹内 裕尚, 高橋 信博

    口腔衛生学会雑誌　56　(4)　609-609　2006/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  95. PCR for detection of mutans streptococci in human dental plaque Invited Peer-reviewed

    Matsuyama J, Sato T, Washio J, Mayanagi G, Ito Y, Abiko Y, Hashimoto K, Miyasawa-Hori H, Nakajo K, Kato K, Takahashi N

    International Congress Series　1284　158-162　2005/09

    DOI： 10.1016/j.ics.2005.07.030 　

    ISSN： 0531-5131

  96. 舌苔中の硫化水素産生菌の網羅的検索と菌種の同定 口臭との関連

    鷲尾 純平, 佐藤 拓一, 小関 健由, 高橋 信博

    Journal of Oral Biosciences　47　(Suppl.)　126-126　2005/09

    Publisher: (一社)歯科基礎医学会

    ISSN： 1349-0079

    eISSN： 1880-3865

  97. 口臭測定の導入が地域の歯科保健活動に果たす役割

    丹田 奈緒子, 岩倉 政城, 井川 恭子, 鷲尾 純平, 草野 歩, 天野 一宇, 今泉 芙由子, 小川 裕平, 山田 雄大, 石井 瑛一郎, 渋谷 芳郎, 芳賀 めぐみ, 鴫原 ヨシ子, 鈴木 健吾, 小関 健由

    口腔衛生学会雑誌　55　(4)　330-330　2005/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  98. 某高等専門学校生のう蝕の状況と歯科受診,フッ化物配合歯磨き剤の使用状況との関係について

    田浦 勝彦, 山田 雄大, 小川 裕平, 天野 一宇, 石井 瑛一郎, 今泉 芙由子, 千葉 順子, 丹田 奈緒子, 井川 恭子, 松坂 朋典, 鷲尾 純平, 小関 健由

    口腔衛生学会雑誌　55　(4)　424-424　2005/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  99. 某高等専門学校生のう蝕罹患状況の改善について

    田浦 勝彦, 鷲尾 純平, 丹田 奈緒子, 井川 恭子, 山田 雄大, 小川 裕平, 天野 一宇, 今泉 芙由子, 石井 瑛一郎, 小関 健由

    口腔衛生学会雑誌　55　(3)　221-221　2005/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  100. 口臭測定の導入が地域歯科保健活動に果たす役割 BreathtronTMによる成人歯科健診受診者への一斉検査

    丹田 奈緒子, 岩倉 政城, 井川 恭子, 鷲尾 純平, 草野 歩, 山田 雄大, 小川 裕平, 天野 一宇, 今泉 芙由子, 石井 瑛一郎, 鈴木 健吾, 小関 健由

    口腔衛生学会雑誌　55　(3)　222-222　2005/07

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  101. LiNbO3を用いた中赤外線波長レーザーの生物学的効果

    鈴木 麻衣子, 鷲尾 純平, 小関 健由

    東北大学歯学雑誌　23　(2)　90-90　2004/12

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  102. 【口臭測定器材】口臭測定器 ブレストロン VSC検出機構と臨床応用

    持田 浩司, 鈴木 健吾, 岩倉 政城, 鷲尾 純平

    DE　(151)　13-14　2004/10

    Publisher: (一社)日本歯科理工学会

    ISSN： 0385-0129

  103. 3歳児のう蝕をきっかけに取り組んだ地域歯科保健計画策定と成人歯科健診

    芳賀 めぐみ, 鷲尾 純平, 井川 恭子, 丹田 奈緒子, 草野 歩, 渋谷 芳郎, 岩倉 政城, 小関 健由

    口腔衛生学会雑誌　54　(4)　372-372　2004/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  104. Breathtronを用いた口臭測定の条件についての検討

    井川 恭子, 鷲尾 純平, 丹田 奈緒子, 針生 ひろみ, 志村 匡代, 草野 歩, 岩倉 政城, 小関 健由

    口腔衛生学会雑誌　54　(2)　152-153　2004/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  105. 住民歯科保健学習組織構成員の助言を契機に始めた歯周病療養の体験報告

    広田 好子, 朝倉 みさ子, 鴫原 ヨシ子, 鷲尾 純平, 小関 健由, 岩倉 政城

    口腔衛生学会雑誌　54　(2)　156-156　2004/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  106. 地域活動体験を経た歯科衛生士の業務進展

    大沼 禎子, 安斉 深雪, 伊東 美香, 折越 文子, 片平 玲子, 鴫原 ヨシ子, 渡辺 淳子, 朝倉 みさ子, 鷲尾 純平, 小関 健由, 岩倉 政城

    口腔衛生学会雑誌　54　(2)　157-157　2004/04

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  107. 口臭と舌苔中の総菌数及び硫化水素産生菌数との関係

    鷲尾 純平, 佐藤 拓一, 井川 恭子, 丹田 奈緒子, 岩倉 政城, 小関 健由, 高橋 信博

    口腔衛生学会雑誌　53　(4)　344-344　2003/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  108. 歯垢中のmutans streptococciのPCR法による検出について

    熊谷 崇, 重光 竜二, 太郎丸 毅, 鷲尾 純平, 小関 健由, 佐藤 拓一, 高橋 信博

    東北大学歯学雑誌　22　(1)　54-54　2003/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  109. 口腔領域の慢性感染症、特に下顎骨骨髄炎及び感染性歯根嚢砲に関する研究． (大和証券ヘルス財団研究業績集)

    佐藤拓一, 高橋信博, 山浦みゆき, 越後成志, 鷲尾純平, 長坂浩, 松山順子, 大島勇人

    大和証券ヘルス財団研究業績集　26　10-14　2003/04

  110. 口臭測定器ブレストロンの開発

    針生 ひろみ, 志村 匡代, 鷲尾 純平, 井川 恭子, 丹田 奈緒子, 岩倉 政城, 鈴木 健吾

    東北大学歯学雑誌　21　(2)　127-127　2002/12

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  111. 東北大学予防歯科口臭外来20年間の受診動態

    鷲尾 純平, 針生 ひろみ, 志村 匡代, 井川 恭子, 丹田 奈緒子, 岩倉 政城

    口腔衛生学会雑誌　52　(5)　718-719　2002/10

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  112. 高専生におけるう蝕有病状況とフッ化物配合歯磨剤の使用状況に関する検討

    井川 恭子, 田浦 勝彦, 針生 ひろみ, 志村 匡代, 丹田 奈緒子, 松坂 朋典, 鷲尾 純平

    口腔衛生学会雑誌　52　(5)　719-719　2002/10

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  113. 某高等専門学校男子学生の1975年から2000年のう蝕有病状況の変化

    井川 恭子, 鷲尾 純平, 楠本 雅子, 松坂 朋典, 針生 ひろみ, 志村 匡代, 丹田 奈緒子, 千葉 潤子, 小澤 雄樹, 田浦 勝彦

    口腔衛生学会雑誌　52　(4)　382-383　2002/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  114. 第一線臨床に向けた口臭測定器の開発

    岩倉 政城, 針生 ひろみ, 鷲尾 純平, 志村 匡代, 井川 恭子, 丹田 奈緒子, 鈴木 健吾

    口腔衛生学会雑誌　52　(4)　456-457　2002/08

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

  115. 臨床理工講座 口臭測定器「ブレストロン」について 口臭臨床における機器測定の意義

    岩倉 政城, 針生 ひろみ, 鷲尾 純平, 志村 匡代, 井川 恭子, 丹田 奈緒子, 鈴木 健吾

    日本歯科評論　(716)　105-108　2002/06

    Publisher: (株)ヒョーロン・パブリッシャーズ

    ISSN： 0289-0909

  116. 予防歯科口臭外来開設以降19年間の受診動態と臨床統計

    針生 ひろみ, 岩倉 政城, 鷲尾 純平, 志村 匡代, 井川 恭子, 丹田 奈緒子, 坂本 征三郎

    東北大学歯学雑誌　21　(1)　43-44　2002/06

    Publisher: 東北大学歯学会

    ISSN： 0287-3915

  117. ZnO厚膜型センサの口臭測定器への応用

    鈴木 健吾, 鷲尾 純平, 岩倉 政城

    Proceedings of the Chemical Sensor Symposium　34　19-21　2002/04

    Publisher: 電気化学会化学センサ研究会

  118. 口臭測定器「ブレストロン」について ─口臭臨床における機器測定の意義―. (日本歯科評論)

    岩倉政城, 針生ひろみ, 鷲尾純平, 志村匡代, 井川恭子, 丹田奈緒子, 鈴木健吾

    日本歯科評論　716　105-108　2002/04

  119. 20年間経年観察した仙台市郊外一中学校生徒のHLD indexの推移

    岩倉 政城, 渋谷 芳郎, 丹田 奈緒子, 鷲尾 純平, 千葉 潤子, 小澤 雄樹, 庵原 義彦, 坂本 征三郎

    口腔衛生学会雑誌　51　(4)　622-623　2001/09

    Publisher: (一社)日本口腔衛生学会

    ISSN： 0023-2831

Show all ︎Show first 5

Books and Other Publications 4

  1. 歯科衛生学辞典

    眞木 吉信, 全国歯科衛生士教育協議会

    永末書店　2019

    ISBN: 9784816013683

  2. 食と微生物の事典

    北本 勝ひこ, 春田 伸, 丸山 潤一, 後藤 慶一, 尾花 望, 齋藤 勝晴

    朝倉書店　2017

    ISBN: 9784254431216

  3. デンタルカリエス : その病態と臨床マネージメント

    Fejerskov O, Kidd Edwina A. M, 高橋 信博, 恵比須 繁之

    医歯薬出版　2013

    ISBN: 9784263444023

  4. 分子生物学歯科小事典

    西沢俊樹, 花田信弘, 今井奨, 西原達次, 高橋信博, 佐藤拓一, 鷲尾純平

    口腔保健協会　2003/04

Presentations 181

  1. Metformin inhibits oral cancer cell growth by altering glucose metabolism.

    Liu S, Washio J, Takahashi N

    The 68th Annual meeting of Japanese Association for Dental Research　2020/11/07

  2. Involvement of oral bacterial metabolism in oral health and diseases- Importance of analyzing bacterial metabolic activity, Invited

    Washio J, Takahashi N

    The 2nd International Cooperation Symposium　2020/10/15

  3. 飲酒を想定した中から高濃度エタノール環境下におけるStreptococcus属によるアセトアルデヒド産生

    互野 亮, 鷲尾 純平, 佐々木 啓一, 高橋 信博

    第62回歯科基礎医学会学術大会

  4. 口腔内細菌叢解析システム;PCR-RFLP法によるプロファイリング

    河内 美帆, 丸山 伸吾, 枡田 菜々子, 佐野 拓人, 涌井 杏奈, 八巻 惠子, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    第62回歯科基礎医学会学術大会

  5. Effect of nitrate;nitrite;on the growth;N;roducing activity of oral Veillonella species

    Wicaksono D. P, Washio J, Takahashi N

  6. The antimicrobial;properties of green;tea-derived epigallocatechin gallate on;Streptococcus mutans

    Han S, Abiko Y, Washio J, Luo Y, Zhang L, Takahashi N

  7. Profiling of Microbiota of Baby-Drinks after Drinking with Artificial Nipples

    Wakui A, Sano H, Kawachi M, Masuda N, Maruyama S, Washio J, Abiko Y, Mayanagi G, Yamaki K, Takahashi N, Okada Y, Sato T

    2020/09/11

  8. 口腔内環境モニタリングに向けた全固体イオン選択電極センサアレイの開発と多変量解析アルゴリズムの検討．

    大久保信吾, 石原昇, 山田哲也, 栁田保子, 真柳弦, 鷲尾純平, 高橋信博

    日本化学会第15回バイオ関連化学シンポジウム　2020/09/08

  9. Metformin Influenced Glucose Metabolic Activity of Cancer Cells.

    Kobayashi Y, Washio J, Shinohara Y, Takahshi N

    The 98h IADR General Session & Exhibition,　2020/03

  10. ニプル（哺乳瓶用乳首）を通した、口腔から液体ミルク内への流入

    涌井杏奈, 佐野拓人, 河内美帆, 鷲尾純平, 安彦友希, 真柳 弦, 高橋信博, 佐藤拓一

    第9回口腔保健用機能性食品研究会　2020/02

  11. Green tea-derived epigallocatechin gallate reduced the acid production and promoted the aggregation of Streptococcus mutans.

    Han S, Abiko Y, Washio J, Luo Y, Zhang L, Takahashi N

    2020/02

  12. 母乳および新生児の口腔内の細菌叢解析：保管による影響について

    佐野拓人, 涌井杏奈, 河内美帆, 森山幸枝, 西方真弓, 鷲尾純平, 安彦友希, 真柳 弦, 高橋信博, 佐藤拓一

    第9回口腔保健用機能性食品研究会　2020/02

  13. Profiling of Microbiota of Liquid Baby Formula after Drinking with Artificial Nipples

    Wakui A, Sano H, Kawachi M, Kato R, Moriyama S, Nishikata A, Washio J, Yamaki K, Sakashita R, Sato T

    4th Asia Pacific Regional Congress of the International Association for Dental Research　2019/11

  14. Profiling System of Oral Biofilm Microbiota Utilizing;PCR-RFLP Analysis

    Sano H, Wakui A, Washio J, Yamaki K, Sato T

    4th Asia Pacific Regional Congress of the International Association for Dental Research　2019/11

  15. (Green tea-derived epigallocatechin gallate inhibited the acid production of Streptococcus mutans and promoted the bacterial aggregation)

    2019/10

  16. 温熱による癌細胞の糖代謝活性への影響

    小林 ゆり, 鷲尾 純平, 篠原 優太, 高橋 信博

    第61回歯科基礎医学会学術大会　2019/10

  17. PCR-RFLP法を用いた口腔内細菌叢解析(プロファイリング)システム

    佐野 拓人, 涌井 杏奈, 八巻 惠子, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    第61回歯科基礎医学会学術大会　2019/10

  18. フッ化物による歯周病関連菌Porphyromonas gingivalisの増殖・代謝の抑制効果

    土門 ひと美, 鷲尾 純平, 安彦 友希, 川嶋 順子, 高橋 信博

    第61回歯科基礎医学会学術大会　2019/10

  19. The Challenge Reports of Oral Biofilm by Young Researchers Profiling of microbiota in baby-drinks and liquid baby formula consumed with an artificial nipple

    Wakui A, Sano H, Kawachi M, Kato R, Washio J, Abiko Y, Mayanagi G, Yamaki K, Takahashi N, Sato T

    2019/10

  20. Which oral environmental factors enhance acetaldehyde production by oral streptococci?

    Tagaino R, Washio J, Abiko Y, Tanda N, Sasaki K, Takahashi N

    The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development (iLIM-4)　2019/10

  21. Nitrate induced;the nitrite;producing activity of oral Veillonella -A possible role of nitrate;oral Veilonella on oral;general health

    Dimas Prasetianto Wicaksono, Washio J, Takahashi N

    The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development (iLIM-4)　2019/10

  22. Nitrate;nitrite;promote the growth;nitrite;roduction of oral Veillonella

    Dimas Prasetianto Wicaksono, Washio J, Takahashi N

    2019/09

  23. Environmental Nitrate/nitrite Accelerated Growth and Increased Nitrite-production of Oral Veillonella

    Dimas Prasetianto Wicaksono, Washio J, Takahashi N

    The 97h IADR General Session & Exhibition　2019/06

  24. Physiological properties of acetaldehyde production from ethanol by oral Streptococcus.

    Tagaino R, Washio J, Abiko Y, Tanda N, Sasaki K, Takahashi N

    The 97h IADR General Session & Exhibition　2019/06

  25. 哺乳瓶用乳首（ニプル）を通して乳児用飲料を飲んだ際の口腔からの逆流について 第8回口腔保健用機能性食品研究会、2019年2月16－17日、新潟杏奈，佐野拓人，河内美帆，高橋彩朱伽，加藤理都，鷲尾純平，安彦友希，石黒和子，真柳 弦，高橋信博，佐藤拓一: 哺乳瓶用乳首（ニプル）を通して乳児用飲料を飲んだ際の，口腔からの逆流について

    涌井杏奈, 佐野拓人, 河内美帆, 高橋彩朱伽, 加藤理都, 鷲尾純平, 安彦友希, 石黒和子, 真柳 弦, 高橋信博, 佐藤拓一

    第8回口腔保健用機能性食品研究会、2019年2月16－17日、新潟　2019/02/17

  26. The Role of Nitrate in Prevention of Oral Diseases by Supporting The Growth and Nitrite Production of Oral Veillonella

    Dimas Prasetianto Wicaksono, Jumpei Washio, Nobuhiro Takahashi

    第8回口腔保健用機能性食品研究会　2019/02/17

  27. 口腔内細菌による糖・エタノール由来のアセトアルデヒド産生と口腔環境

    互野 亮, 鷲尾純平, 安彦友希, 丹田奈緒子, 佐々木啓一, 髙橋信博

    第8回口腔保健用機能性食品研究会　2019/02/17

  28. Carbohydrate metabolism of a novel caries-associated bacterium Scardovia wiggsiae.

    Kameda M, Abiko M, Washio J, Takahashi N

    International Symposium for Multimodal research and Education in IOHS-Liaison 2019.　2019/01/11

  29. Acetaldehyde production by oral Streptococcus and Neisseria depending on oral environmental factors

    Tagaino R, Washio J, Abiko Y, Tanda N, Sasaki K, Takahashi N

    International Symposium for;Multimodal research;Education in IOHS-Liaison　2019/01/11

  30. Electrochemical evaluation of the hydrogen peroxide-and fluoride-induced corrosive property and its recovery on the titanium surface.

    Sato S, Fukushima A, Mayanagi G, Sasaki K, Takahashi N

    International;Symposium for;Multimodal research;Education in IOHS-Liaison 2019　2019/01/11

  31. A novel method to screen and isolate nitrite-producing bacteria from the oral cavity

    Washio J, Suzuki Y, Sato T, Akiba M, Igarashi A, Kimura S, Tezuka S, Nakazato K, Takahashi N

    International Symposium for Multimodal research and Education in IOHS-Liaison 2019. 11-12 January, 2019　2019/01/11

  32. Profiling of Microbiota of Baby-Drinks after Drinking with Artificial Nipples.

    Wakui A, Sano H, Aihara H, Kawachi M, Takahashi A, Washio J, Abiko Y, Mayanagi G, Ishiguro K, Yamaki K, Takahashi N, Sato T

    JADR2018/ 66th Japanese Division Meeting 2018　2018/11/17

  33. ニプルを通して飲んだ際の、ベビー飲料内への口腔細菌の流入

    相原 瞳, 涌井杏奈, 佐野拓人, 河内美帆, 鷲尾純平, 安彦友希, 真柳 弦, 八巻恵子, 高橋信博, 佐藤拓一

    第60回歯科基礎医学会学術大会　2018/09/05

  34. Environmental nitrate increased the nitrite production of oral Veillonella

    2018/09/05

  35. 口腔細菌は弱アルカリ性好気環境で高いアセトアルデヒド産生を示す

    口腔細菌は弱アルカリ性好気環境で高いアセトアルデヒド産生を示す

    第60回歯科基礎医学会学術大会　2018/09/05

  36. 健康長寿を育む歯学教育コンソーシアム 東北大学の取り組み （第4報）

    真柳弦, 高橋 信博, 笹野 泰之, 福本 敏, 服部 佳功, 佐々木 啓一

    第37回日本歯科医学教育学会総会および学術大会　2018/07/27

  37. Anti-acidogenic Effects of Licorice Extracts on Oral Biofilm Bacteria.

    Kitagori H, Washio J, Abiko Y, Tagaino R, Takahashi N

    The 96h IADR General Session & Exhibition　2018/07/25

  38. 山本 祐慈, 清水 弘一, 五十嵐 公英, 鷲尾 純平, 高橋 信博： プラークのもつ硝酸還元活性と亜硝酸還元活性(会議録) 第67回 日本口腔衛生学会総会、2018年5月18日

    山本 祐慈, 清水 弘一, 五十嵐 公英, 鷲尾 純平, 高橋 信博

    第67回 日本口腔衛生学会総会　2018/05/18

  39. 口腔細菌の酸産生抑制作用に着目した天然素材の探索

    北郡 秀晃, 鷲尾 純平, 安彦 友希, 髙橋 信博

    第8回口腔保健用機能性食品研究会　2018/02/17

  40. ペットボトルの口の部分に付着する細菌および飲料物中の細菌の量および構成の解析（第 2 報）

    河内美帆, 佐野拓人, 涌井杏奈, 平吹有香, 曾田彩花, 竹中佑太, 米田茜音, 中畑那奈, 相原 瞳, Vidanapathirana GU, 佐藤拓一, 鷲尾純平, 安彦友希, 石黒和子, 真柳 弦, 高橋信博

    第7回口腔保健用機能性食品研究会　2018/01/21

  41. Science of Oral Biofilm- From a metabolic view- Invited

    Washio J, Takahashi N

    Japan-Mongolia dental;forum in Sendai　2018/01/16

  42. Microbiota Profiling at the Mouth of Plastic Bottles after Drinking Straight from Bottles.

    Sano H, Aida A, Vidanapathirana G.U, Wakui A, Takenaka Y, Kawachi M, Aihara H, Washio J, Abiko Y, Mayanagi G, Ishiguro K, Yamaki K, Takahashi N, Sato T

    International Symposium for Multimodal research and Education in IOHS-Liaison 2018　2018/01/13

  43. A novel method to screen and isolate nitrate-producing bacteria from the oral cavity

    Washio J, Suzuki Y, Dimas Prasetianto Wicaksono, Ishiguro K, Nishino M, Otake T, Sasaki S, Tadokoro S, Takahashi S, Ishiguro K, Takahashi N

    International Symposium for Multimodal research and Education in IOHS-Liaison 2018　2018/01/13

  44. Effect of environmental nitrate on the nitrite-producing activity of oral Veillonella.

    Dimas Prasetianto Wicaksono, Washio J, Takahashi N

    International Symposium for Multimodal research and Education in IOHS-Liaison 2018　2018/01/13

  45. Fluoride inhibition on the growth and metabolism of Porphyromonas gingivalis

    Fluoride inhibition on, the growth, metabolism of Porphyromonas gingivalis

    International;Symposium for Multimodal research and Education in IOHS-Liaison　2018/01/13

  46. Acid production and its fluoride tolerance of a novel caries-associated bacterium Scardovia wiggsiae.

    Kameda M, Abiko M, Washio J, Takahashi N

    International Symposium for Multimodal research and Education in IOHS-Liaison 2018.　2018/01/13

  47. Carbohydrate metabolism related to cariogenicity of oral Bifidobacteirum.

    Abiko Y, Kameda M, Nagai K, Sugahara A, Murakami K, Kawashima J, Washio J, Takahashi N

    International Symposium for Multimodal research and Education in IOHS-Liaison 2018.　2018/01/13

  48. A microbiological study on bacteria in the PET Bottles after Drinking.

    Aida A, Sano H, Wakui A, Hirabuki Y, Takeyama Y, Kawachi M, Vidanapathirana G, Washio J, Sato T

    The 65 th Annual meeting of Japanese Association for Dental Research　2017/11/18

  49. Fluoride inhibits the growth of Porphyromonas gingivalis

    Domon H, Washio J, Takahashi N

    The 65th Annual meeting of Japanese Association for Dental Research　2017/11/18

  50. pH response and tooth surface solubility at the tooth/bacteria interface

    Mayanagi G, Igarashi K, Washio J, Takahashi N

    The 2nd International Symposium on Biomedical Engineering　2017/11/09

  51. スマートデバイスを用いた口腔微小環境マルチイオン測定

    真柳弦, 五十嵐公英, 鷲尾純平, 土門ひと美, 高橋信博, 柳田保子, 伊藤浩之, 石原昇, 益一哉

    東工大未来研;東北大歯学研究科;包括的研究協力協定 第5回研究会　2017/10/23

  52. 飲料物の飲み口に付着する細菌の量および構成の解析

    佐野 拓人, 平吹 有香, 涌井 杏奈, 曾田 彩花, 安彦 友希, 八巻 恵子, 真柳 弦, 鷲尾 純平, 高橋 信博, 佐藤 拓一

    第59回歯科基礎医学会学術大会　2017/09/16

  53. Effect of lactate and pH on nitrite producing activity of Veillonella atypica

    2017/09/16

  54. 口腔バイオフィルム細菌の増殖・代謝抑制作用を有する天然植物抽出物の探索

    北郡秀晃, 鷲尾純平, 互野亮, 高橋信博

    第59回歯科基礎医学会学術大会　2017/09/16

  55. Carbohydrate metabolism of;a;novel caries-associated bacterium Scardovia wiggsiae―A possible role of acetic acid;bacteria in;the;dental caries etiology

    2017/09/16

  56. 健康長寿を育む歯学教育コンソーシアム 東北大学の取り組み （第3報）

    真柳弦, 高橋 信博, 笹野 泰之, 福本 敏, 服部 佳功, 佐々木 啓一

    第36回日本歯科医学教育学会総会および学術大会　2017/07/28

  57. グルコース添加によるプラークの硝酸還元活性の増加と酸産生活性の抑制

    山本祐慈, 五十嵐公英, 清水弘一, 鷲尾純平, 高橋信博

    第66回日本口腔衛生学会・総会　2017/06/02

  58. Lactose and glucose metabolism of oral Bifidobacterium and its fluoride-inhibition

    Manome A, Abiko Y, Kawashima J, Fukumoto S, Takahashi N

    The 95h IADR General Session & Exhibition　2017/03/22

  59. 2-deoxy-D-glucose and dichloroacetate affect the glucose metabolic system of OSCC.

    Kitamura J, Washio J, Morishima H, Takahashi T, Takahashi N

    , The 95h IADR General Session & Exhibition　2017/03/22

  60. Glucose, glutamine and glutamate metabolism of OSCC cells and pH

    Morishima H, Kitamura J, Washio J, Takahashi T, Takahashi N

    The 95h IADR General Session & Exhibition　2017/03/22

  61. スマートISFETを用いた口腔微小環境マルチイオン測定

    真柳弦, 五十嵐公英, 鷲尾純平, 中條和子, 土門ひと美, 高橋信博

    東北大歯学研究科－東工大未来研学術連携シンポジウム　2017/03/09

  62. ペットボトルの口の部分に付着する細菌の量および構成（A pilot study）

    曾田彩花, 佐藤拓一, 石黒和子, 安彦友希, 真柳 弦, 鷲尾純平, 高橋信博

    第6回 口腔保健用機能性食品研究会・総会　2017/01/29

  63. ISFETのう蝕研究への展開

    真柳弦, 五十嵐公英, 鷲尾純平, 中條和子, 土門ひと美, 高橋信博

    第5回生体医歯工学公開セミナー　2016/12/22

  64. Inhibitory effects of sodium dicloroacetate and 2-Deoxy-D-glucose on the acid producing activity of oral squamous cell carcinoma.

    北村淳, 鷲尾純平, 森島浩允, 髙橋哲, 髙橋信博

    第61回 公益社団法人 日本口腔外科学会総会・学術大会　2016/11/25

  65. Evaluation of pH using an ISFET at the bacteria/restorative materials interface

    Mayanagi G, Igarashi K, Washio J, Nakajo K, Domon H, Takahashi N

    International Symposium on Biomedical Engineering　2016/11/10

  66. 代謝モニタリング・システムを用いた癌細胞の糖・アミノ酸代謝活性測定と代謝活性に対する環境pHの影響.

    森島 浩允, 鷲尾 純平, 北村 淳, 高橋 哲, 高橋 信博

    第58回歯科基礎医学会学術大会　2016/08/25

  67. 長期使用義歯からの細菌由来揮発性代謝産物の検出

    互野 亮, 末永 華子, 鷲尾 純平, 佐々木 啓一, 高橋 信博

    第58回歯科基礎医学会学術大会　2016/08/25

  68. 口腔扁平上皮癌のグルコース代謝に対するジクロロ酢酸ナトリウム及び2-デオキシ-D-グルコースの抑制効果

    北村 淳, 鷲尾 純平, 森島 浩允, 高橋 哲, 高橋 信博

    第58回歯科基礎医学会学術大会　2016/08/25

  69. 鷲尾 純平, 石黒 和子, 入江 大貴, 内山 愛理, 高橋 信博: 蛍光色素を用いた口腔プラークバイオフィルム代謝活性の高感度測定法.

    鷲尾 純平, 石黒 和子, 入江 大貴, 内山 愛理, 高橋 信博

    第58回歯科基礎医学会学術大会　2016/08/25

  70. 亜硝酸塩による口腔内細菌に対する酸産生抑制機構, 第５回 口腔保健用機能性食品研究会・総会，2016年1月24日

    鷲尾純平, 山本祐慈, 高橋信博

    第５回 口腔保健用機能性食品研究会・総会　2016/01/24

  71. A high sensitive fluorescence-mediated assessment of plaque metabolic activity.

    Washio J, Ishiguro K, Irie D, Uchiyama A, Takahashi N

    Innovative Research for Biosis-Abiosis Intelligent Interface Symposium: The 6th International Symposium for Interface Oral Health Science　2016/01/18

  72. PCR-dipstick DNA chromatography for multiplex and semi-quantitative analysis of plaque biofilm microbiota.

    Tian L, Sato T, Niwa K, Kawase M, Mayanagi G, Abiko Y, Washio J, Takahashi N

    Innovative Research for Biosis-Abiosis Intelligent Interface Symposium: The 6th International Symposium for Interface Oral Health Science　2016/01/18

  73. Effects of pH on the glucose metabolism of oral squamous cell carcinoma cells.

    Morishima H, Washio J, Kitamura J, Ogawa T, Takahashi T, Takahashi N

    Innovative Research for Biosis-Abiosis Intelligent Interface Symposium: The 6th International Symposium for Interface Oral Health Science　2016/01/18

  74. Inhibitory effects of 2-deoxy-D-glucose on the glucose metabolism of oral squamous cell carcinoma cells.

    Kitamura J, Washio J, Morishima H, Ogawa T, Takahashi T, Takahashi N

    Innovative Research for Biosis-Abiosis Intelligent Interface Symposium: The 6th International Symposium for Interface Oral Health Scienceffects of 2-deoxy-D-glucose on the glucose metabolism of oral squamous cell carcinoma cells. Innovative Research for Biosis-Abiosis Intelligent Interface Symposium: The 6th International Symposium for Interface Oral Health Science　2016/01/18

  75. 口腔疾患の発生機序解明への新たなアプローチ

    鷲尾純平, 小川珠生, 北村 淳, 森島浩允, 田 玲鍚, 石黒和子, 真柳 弦, 安彦友希, 佐藤拓一, 高橋信博

    第68回東北大学歯学会　2015/12/11

  76. Effects of pH on the glucose metabolism of cancer cells.

    Morishima H, Washio J, Kitamura J, Ogawa T, Kawai T, Takahashi T, Takahashi N

    The 63rd Annual Meeting of the Japanese Association for Dental Research　2015/10/31

  77. A novel method for real-time monitoring of bacterial metabolic activity.

    Ishiguro K, Washio J, Sasaki K, Takahashi N

    The 93rd IADR General Session & Exhibition　2015/03/12

  78. Nitrite inhibition on streptococcal acid-production and its biochemical mechanism.

    Washio J, Yamamoto Y, Takahashi N

    The 93rd IADR General Session & Exhibition　2015/03/12

  79. 亜硝酸塩のStreptococcus mutans酸産生抑制機構をメタボロミクスで解明する

    鷲尾純平, 山本祐慈, 高橋信博

    第56回歯科基礎医学会学術大会　2014/09/27

  80. 蛍光色素を用いた細菌の代謝活性リアルタイム・モニター法の確立

    石黒和子, 鷲尾純平, 佐々木啓一, 高橋信博

    第56回歯科基礎医学会学術大会　2014/09/26

  81. Oral microbiota in crevices around dental implants: profiling of the oral biofilm.

    Sato T, Kawamura Y, Mayanagi G, Washio J, Takahashi N

    Sato T, Kawamura Y, Mayanagi G, Washio J, Takahashi N　2014/01/20

  82. ヒト・プラークの糖アルコール代謝：メタボロミクス的アプローチ

    鷲尾純平, 高橋信博

    第55回歯科基礎医学会学術大会　2013/09/22

  83. メタボローム解析による口腔扁平上皮癌のエネルギー代謝特性の解明とバイオマーカーの探索

    小川珠生, 鷲尾純平, 高橋 哲, 高橋信博

    第55回歯科基礎医学会学術大会　2013/09/21

  84. Involvement of tooth surface solubility in Stephan curve.

    Mayanagi G, Igarashi K, Washio J, Takahashi N

    The 2nd IADR-APR (Asia Pacific Region)　2013/08/21

  85. Pathognomonic Glutamine Metabolism in Oral Squamous Cell Carcinoma –Metabolomic Approach-

    Ogawa T, Washio J, Takahashi T, Takahashi N

    Ogawa T, Washio J, Takahashi T, Takahashi N: Pathognomonic Glutamine Metabolism in Oral Squamous Cell Carcinoma –Metabolomic Approach- The 2nd IADR-APR (Asia Pacific Region)　2013/08/21

  86. Stephan curve and tooth surface solubility at tooth-bacteria interface.

    Mayanagi G, Igarashi K, Washio J, Takahashi N

    91st IADR　2013/03/23

  87. プラークと唾液のメタボローム解析 －口腔内でのアミノ酸の利用に注目して－

    鈴木啓佑, 月星陽介, 渡邊基博, 小川珠生, 鷲尾純平, 高橋信博

    第22回日本歯科医学会総会　2012/11/11

  88. メタボローム解析で見えてきた口腔バイオフィルムの機能 －「何がいるか」から「何をしているか」－

    鷲尾純平, 真柳弦, 小川珠生, 高橋信博

    第22回日本歯科医学会総会　2012/11/10

  89. メタボローム解析から見た口腔扁平上皮癌の糖・アミノ酸代謝の特徴

    小川珠生, 鷲尾純平, 高橋 哲, 高橋信博

    第57回日本口腔外科学会総会・学術大会　2012/10/19

  90. 口腔プラークバイオフィルム中のアミノ酸組成およびその代謝に伴うアンモニア産生に関するメタボロミクス的考察

    鷲尾純平, 月星陽介, 渡邊基博, 鈴木啓佑, 高橋信博

    第7回メタボロームシンポジウム　2012/10/10

  91. The composition and the utilization of amino acids in dental plaque biofilm -Metabolomics approach with CE-TOFMS-

    2012/09/16

  92. The method to quantify the amount of periodontitis-associated bacteria adhered to biomaterials using fluorescent dye, alamar Blue®. 第54回歯科基礎医学会学術大会石黒和子，鷲尾純平，佐久間陽子，竹内裕尚，佐々木啓一，高橋信博： 蛍光色素alamar Blue®を用いたバイオマテリアル付着歯周病関連細菌定量法 The method to quantify the amount of periodontitis-associated bacteria adhered to biomaterials using fluorescent dye, alamar Blue®.

    2012/09/15

  93. Metabolome analysis of oral squamous cell carcinoma using CE-TOFMS.

    Ogawa T, Washio J, Echigo S, Takahashi N

    IADR 2012　2012/06/22

  94. Amino acid composition and amino-acid degrading activity of dental plaque.

    Washio J, Ogawa T, Suzuki K, Tsukiboshi Y, Watanabe M, Takahashi N

    IADR 2012　2012/06/21

  95. バイオマテリアル－パラサイト・インターフェイスへの挑戦－バイオマテリアルの生物学的劣化の理解へ－．

    バイオマテリアル-パラサイト, インターフェイスへの挑戦-バイオマテリアルの生物学的劣化の理解へ

    九州大学応用力学研究所平成23年度共同利用研究集会「力学適応能、自己組織化能を有するバイオマテリアル－生体インターフェイスの創製」　2012/03/02

  96. CE-TOFMSを用いた口腔プラークバイオフィルムの糖代謝メタボローム解析～フッ化物やキシリトールによる影響をin vivoで探る～．

    鷲尾純平, 小川珠生, 真柳 弦, 高橋信博

    キャピラリー電気泳動シンポジウム　2011/11/09

  97. イオン感受性電界効果型トランジスタ微小pH電極による細菌－歯科修復材料・インターフェイスpH測定．

    真柳 弦, 五十嵐公英, 鷲尾純平, 中條和子, 土門-俵谷ひと美, 高橋信博

    粉体粉末冶金協会 平成23年度秋季大会（第108回講演大会）　2011/10/26

  98. Fluoride-tolerance of Actinomyces acid production and its enhancement by bicarbonate.

    Kawashima J, Nakajo K, Washio J, Shimauchi H, Takahashi N

    第59回JADR学術大会　2011/10/08

  99. Evaluation of pH at the bacteria-restorative material interface.

    Mayanagi G, Igarashi K, Washio J, Nakajo K, Domon-Tawaraya H, Takahashi N

    第59回JADR学術大会（広島）　2011/10/08

  100. ヒト・プラークの糖代謝に対するフッ化物およびキシリトールの影響～CE-TOFMSを用いたメタボロミクス・アプローチ～．

    鷲尾純平, 高橋信博

    第53回歯科基礎医学会学術大会　2011/10/01

  101. ヒトプラークとう蝕関連口腔細菌Streptococcus mutansの糖代謝に対するキシリトールの影響の相違．

    鷲尾純平, 高橋信博

    第84回日本生化学大会　2011/09/24

  102. Evaluation of pH using an ISFET at the interface between bacteria and restorative materials.

    Mayanagi G, Igarashi K, Washio J, Nakajo K, Domon-Tawaraya H, Takahashi N

    The 6th International Workshop on Nano-, Bio- and Amorphous Materials　2011/08/08

  103. 根面齲触関連菌Actinomycesの酸産生活性とそれに及ぼす環境pH、重炭酸、フッ化物の影響

    川嶋順子, 中條和子, 鷲尾純平, 島内英俊, 高橋信博

    日本歯科保存学会第134回学術大会　2011/06/09

  104. 口腔環境を考慮した糖負荷試験時呼気中アセトン測定と血中ケトン体との関連．

    丹田奈緒子, 檜尾好徳, 由浪有希子, 鷲尾純平, 高橋信博, 小関健由

    54回日本糖尿病学会年次学術集会　2011/05/19

  105. Evaluation of pH using an ISFET at the parasite-biomaterial interface.

    Mayanagi G, Igarashi K, Washio J, Nakajo K, Domon-Tawaraya H, Takahashi N

    The 89th IADR　2011/03/17

  106. Metabolome analysis of oral plaque biofilm using CE-TOFMS.

    Washio J, Mayanagi G, Takahashi N

    The 4th International Symposium for Interface Oral Health Science (Sendai)　2011/03/08

  107. Breath acetone in type 1 and type 2 diabetes mellitus.

    Tanda N, Hinokio Y, Washio J, Takahashi N, Koseki T

    The 4th International Symposium for Interface Oral Health Science (Sendai)　2011/03/08

  108. A high-sensitive alamarBlue® method for evaluating bacterial adhesion to biomaterials.

    Sakuma Y, Washio J, Takeuchi Y, Sasaki K, Takahashi N

    The 4th International Symposium for Interface Oral Health Science (Sendai)　2011/03/08

  109. Evaluation of pH at the interface between bacteria and restorative materials.

    Mayanagi G, Igarashi K, Washio J, Nakajo K, Domon-Tawaraya H, Takahashi N

    The 4th International Symposium for Interface Oral Health Science (Sendai)　2011/03/08

  110. Actinomyces acid production: Effects on bicarbonate and fluoride at neural and acid pH.

    Kawashima J, Nakajo K, Washio J, Shimauchi H, Takahashi N

    The 4th International Symposium for Interface Oral Health Science (Sendai)　2011/03/08

  111. Divalent cations enhance short-time fluoride exposure-induced inhibition on acid production by oral streptococci.

    Domon-Tawaraya H, Nakajo K, Washio J, Fukumoto S, Takahashi N

    The 4th International Symposium for Interface Oral Health Science　2011/03/08

  112. 口腔フローラのプロファイリング

    佐藤拓一, 安彦友希, 長谷川彩子, 真柳 弦, 鷲尾純平, 中條和子, 堀(宮澤)はるみ, 高橋信博

    第58回東北大学歯学会　2010/12/10

  113. Divalent cations enhance short-time fluoride exposure-induced inhibition on streptococcal acidogenicity.

    Domon-Tawaraya H, Nakajo K, Washio J, Fukumoto S, Takahashi N

    第58回JADR学術大会　2010/11/20

  114. Metabolome analysis of oral plaque biofilm –sugar metabolism and amino acid metabolism-

    2010/09/21

  115. Effects of divalent metal ions on the short-term fluoride exposure-induced inhibition of the acid production of Streptococcus mutans

    2010/09/21

  116. Metabolome analysis of glucose fermentation by dental plaque using CE-TOFMS.

    Washio J, Mayanagi G, Katsuda Y, Hata T, Sakurada K, Sato K, Tsuji N, Takahashi N

    Washio J, Mayanagi G, Katsuda Y, Hata T, Sakurada K, Sato K, Tsuji N and Takahashi N: Metabolome analysis of glucose fermentation by dental plaque using CE-TOFMS. The 88th IADR　2010/07/16

  117. An improved alamarBlue® method for evaluating bacterial adhesion.

    Sakuma Y, Washio J, Takeuchi Y, Sasaki K, Takahashi N

    The 88th IADR　2010/07/16

  118. Acetone in mouth air in type 2 diabetes mellitus

    Tanda N, Hinokio Y, Washio J, Takahashi N, Koseki T

    The 88th IADR　2010/07/16

  119. 入院中に口腔環境改善を試みた治療コンプライアンスの低い2型糖尿病の1例

    丹田奈緒子, 檜尾好徳, 由浪有希子, 鷲尾純平, 高橋信博, 小関健由, 片桐秀樹, 岡芳知

    第53回日本糖尿病学会年次学術集会　2010/05/27

  120. ISFETによるバイオフィルム－マテリアルインターフェイス環境測定

    真柳 弦, 五十嵐公英, 鷲尾純平, 高橋信博

    第５回高機能インターフェイス科学カンファレンス　2010/03/14

  121. Metabolome analysis of glucose metabolic pathways of dental caries-associated oral bacteria: similarity and difference in functional glucose metabolism

    2009/10/22

  122. CE-TOFMSを用いたプラークバイオフィルムの糖代謝メタボローム解析

    鷲尾純平, 高橋信博

    第58回日本口腔衛生学会・総会　2009/10/10

  123. Quantitative fluorometric method for evaluating oral bacterial adhesion to biomaterials.

    Sakuma Y, Washio J, Takeuchi Y, Sasaki K, Takahashi N

    The 2nd Meeting of IADR Pan Asian Pacific Federation (PAPF) and the 1st Meeting of IADR Asia/Pacific Region (APR),　2009/09/24

  124. Actinomyces glycolysis: Fluoride tolerance and the effect of bicarbonate on the sensitivity

    2009/09/10

  125. Metabolome analysis of glucose fermentation by Streptococcus mutans and Actinomyces naeslundii using CE-TOFMS

    2009/09/10

  126. 口腔バイオフィルムのメタボローム解析の試み ～何がいるかから、何をしているかへ～

    鷲尾純平, 真柳 弦, 高橋信博

    第51回歯科基礎医学会学術大会サテライトシンポジウム　2009/09/09

  127. Short-term inhibition of plaque acidogenicity by NaF-mouthrinse and fluoride retention within dental plaque.

    Takahashi N, Nakajo K, Asanoumi T, Shibata A, Yagishita Y, Washio J

    ORCA 2009　2009/07/01

  128. Alamar blue®を用いたバイオマテリアル表面細菌付着量の新たな評価法の確立

    佐久間陽子, 鷲尾純平, 竹内裕尚, 佐々木啓一, 高橋信博

    第6回東北大学バイオサイエンスシンポジウム　2009/06/16

  129. 口腔常在菌VeillonellaやActinomycesによるシステインおよびシステイン含有ペプチドからの口臭成分（硫化水素）産生と、それに対する環境pH、乳酸の影響について

    鷲尾純平, 小野瞳, 高野郁子, 高橋信博

    第6回東北大学バイオサイエンスシンポジウム　2009/06/16

  130. Difference between age generations about impression of oral health examination in a rural town.

    Tanda N, Ikawa K, Washio J, Shigihara Y, Shibuya Y, Iwakura M, Haga M, Ogawa Y, Taura K, Koseki T

    The 3 rd International Symposium for Interface Oral Health Science　2009/01/15

  131. Short-term effect of fluoride on acid production by Streptococcus mutans

    Domon H, Nakajo K, Washo J, Miyasawa-Hori H, Fukumoto S, Takahashi N

    The 3 rd International Symposium for Interface Oral Health Science　2009/01/15

  132. Hydrogen-sulfide production from various substrates by oral Veillonella and effects of lactate on the production

    Washio J, Sakuma Y, Shimada Y, Takahashi N

    The 3 rd International Symposium for Interface Oral Health Science　2009/01/15

  133. 口腔Veillonellaはcysteine含有ペプチドから硫化水素を産生し、乳酸はその硫化水素産生を促進する

    鷲尾 純平, 佐久間 陽子, 島田 裕子, 高橋 信博

    歯科基礎医学会学術大会・総会　2008/09/23

  134. バイオマテリアル表面への各種口腔細菌付着量の評価法

    佐久間 陽子, 鷲尾 純平, 竹内 裕尚, 佐々木 啓一, 高橋 信博

    第50回歯科基礎医学会学術大会　2008/09/23

  135. Quantitative fluorometry for evaluating oral bacterial adhesion to biomaterials International-presentation

    Yoko Sakuma, Jumpei Washio, Yasuhisa Takeuchi, Keiichi Sasaki, Nobuhiro Takahashi

    3rd International Symposium onNANO-BIO and AMORPHOUS MATERIALS　2008/08/07

  136. Lactate enhances the production of hydrogen sulfide by oral Veillonella International-presentation

    Jumpei Washio, Azusa Fukushima, Tamaki Ogawa, Satoshi Okada, Nobuhiro Takahashi

    ＩＡＤＲ 国際歯科学会　2008/07/02

  137. 口腔常在菌Veillonellaはシステイン含有ペプチドから口臭の原因となる硫化水素を産生し、その産生量は口腔内の乳酸によって増加する

    鷲尾純平, 島田裕子, 坂巻良一, 山田雅一, 岡田諭, 小川珠生, 福島梓, 高橋信博

    第5回東北大学バイオサイエンスシンポジウム　2008/05/19

  138. 口腔環境中の乳酸はVeillonellaの硫化水素産生を促進する

    鷲尾純平, 高橋信博

    第５６回日本口腔衛生学会　2007/10/03

  139. 舌苔細菌と口臭〜舌苔常在細菌の口臭産生への関与〜

    鷲尾純平, 佐藤拓一, 中條和子, 高橋信博

    歯科基礎医学会学術大会　2007/08/29

  140. 口腔Veillonellaはcystathionine β-synthase を持ちシステインを分解して硫化水素を産生する

    鷲尾純平, 高橋信博

    歯科基礎医学会　2007/08/29

  141. Effects of pH and lactate on the production of hydrogen sulfide by oral Veillonella International-presentation

    Jumpei Washio, Yuko Shimada, Ryoichi Sakamaki, Masakazu Yamada, Nobuhiro Takahashi

    International Society of Breath Odor Research　2007/08/23

  142. 舌苔中のどのような細菌が、どのように口臭を産生しているのか？

    鷲尾 純平, 高橋 信博

    口鼻臭臨床研究会　2007/06/23

  143. The Hydrogen sulfide production by Oral Veillonella: effects of substrate and environmental pH International-presentation

    J.Washio, K.Nakajo, T. Sato, S. Matoba, T. Seki, N. Yamamoto, M. Yamamoto, N. Takahashi

    International Symposium for Interface Oral Health Science　2007/02/18

  144. Inhibitory effects of maltotriitol on the growth and the adhesion of mutans streptococci International-presentation

    H. Miyasawa-Hori, S. Aizawa, J. Washio, N. Takahashi

    International Symposium for Interface Oral Health Science　2007/02/18

  145. Psychological Characteristics of halitosis patients by using Egogram and the halitosis International-presentation

    Ayumi Kusano, Masaki Iwakura, Kyoko Ikawa, Naoko Tanda, Jumpei Washio, Yuhei, Ogawa, Yudai Yamada, Takeyoshi Kosek, i

    International Symposium for Interface Oral Health Science　2007/02/18

  146. Dental examinations for oral health promotion in a rural town International-presentation

    N. Tanda, M. Iwakura, K. Ikawa, J. Washio, A. Kusano, K. Amano, Y. Ogawa, Y. Yamada, Y. Shigihara, Y. Shibuya, M. Haga, K. Osaka, T. Koseki

    International Symposium for Interface Oral Health Science　2007/02/18

  147. 唾液細菌および口腔Veillonellaによる口臭成分の一つ硫化水素の産生とその唾液や口腔環境との関わり

    鷲尾純平, 佐藤拓一, 竹内裕尚, 高橋信博

    日本口腔衛生学会　2006/10/06

  148. 口腔Veillonellaによる硫化水素産生に関する検討―菌種・基質・環境pHによる違い？

    鷲尾純平, 高橋信博

    第48回歯科基礎医学会学術大会　2006/09/21

  149. Metabolic Properties of Hydrogen Sulfide Production by Oral Veillonella International-presentation

    J.Washio, K.Nakajo, T. Sato, S. Matoba, T. Seki, N. Yamamoto, M. Yamamoto, N. Takahashi

    International Association for Dental Research　2006/06/30

  150. pH-tolerant Acid Production from glucose by Enterococcus faecalis International-presentation

    K. Nakajo, J. Washio, S. Aizawa, H. Miyasawa-Hori, T. Sato, N. Takahashi

    International Association for Dental Research　2006/06/30

  151. Psychological Characteristics of halitosis patients by using Egogram and Questionnaires International-presentation

    A. Kusano, M. Iwakura, K .Ikawa, N. Tanda, J. Washio, T.Koseki

    International Association for Dental Research　2006/06/30

  152. Easy Measurement of Unstimulated Saliva by Modified Swab Method International-presentation

    Y. Ogawa, A. Kusano, Y. Yamada, K. Amano, K. Ikawa, N. Tanda, Y. Shigihara, E. Ito, J. Washio, K. Taura, M. Iwakura, T. Koseki

    International Association for Dental Research　2006/06/30

  153. 津山町で実施した学習歯科健診の試み

    丹田奈緒子, 岩倉政城, 井川恭子, 鷲尾純平, 草野歩, 天野一宇, 小川裕平, 山田雄大, 鴫原ヨシ子, 渋谷芳郎, 小関健由, 阿部, 陽子, 小橋純子, 渋谷香奈, 小泉香織, 成澤

    第42回宮城県公衆衛生学会学術総会　2006/06/30

  154. 口臭測定の導入が地域の歯科保健活動に果たす役割

    丹田奈緒子, 岩倉政城, 井川恭子, 鷲尾純平, 草野 歩, 天野一宇, 今泉芙由子, 小川裕平, 山田雄大, 石井瑛一郎, 渋谷芳郎, 芳賀めぐみ, 鴫原ヨシ子, 鈴木健吾, 小関健由

    第54回日本口腔衛生学会・総会　2005/10/06

  155. 某高等専門学校生のう蝕の状況と歯科受診、フッ化物配合歯磨き剤の使用状況との関係について

    田浦勝彦, 山田雄大, 小川裕平, 天野一宇, 石井瑛一郎, 今泉芙由子, 千葉潤子, 丹田奈緒子, 井川恭子, 松阪朋典, 鷲尾純平, 小関健由

    第54回日本口腔衛生学会・総会　2005/10/06

  156. 舌苔中の硫化水素産生菌の網羅的検索と菌種の同定？口臭との関連？

    鷲尾純平, 佐藤拓一, 小関健由, 高橋信博

    歯科基礎医学会　2005/09/28

  157. 口腔微生物叢の重要性：研究の現在と未来

    佐藤拓一, 鷲尾純平, 中條和子, 堀(宮澤)はるみ, 松山順子, 高橋信博

    第47回歯科基礎医学会学術大会　2005/09/28

  158. 某専門学校生のう蝕罹患状況の改善について

    田浦勝彦, 鷲尾純平, 丹田奈緒子, 井川恭子, 山田雄大, 小川裕平, 天野一宇, 今泉芙由子, 石井瑛一郎, 小関健由

    第17回日本口腔衛生学会東北地方会　2005/05/28

  159. 口臭測定の導入が地域の歯科保健活動に果たす役割―BreathtronTMによる成人歯科健診受診者への一斉検査―

    丹田奈緒子, 岩倉政城, 井川恭子, 鷲尾純平, 草野 歩, 山田雄大, 小川裕平, 天野一宇, 今泉芙由子, 石井瑛一郎, 鈴木健吾, 小関健由

    第17回日本口腔衛生学会東北地方会　2005/05/28

  160. A residents-oriented dental health program in a rural town. International-presentation

    M. Iwakura, M. Haga, K. Ikawa, J. Washio, N. Tanda, A. Kusano, Y. Shibuya, Y. Shigihara, K. Suzuki, T. Koseki

    第83回International Association for Dental Research　2005/03/10

  161. Circadian changes of volatile sulfur compounds measured by Breathtron. International-presentation

    K. Ikawa, M. Iwakura, J. Washio, A. Kusano, N. Tanda, T. Koseki

    International Symposium for Interface Oral Health Science　2005/02/03

  162. Relationship between hydrogen sulfide-producing bacteria of the tongue coating and oral malodor. International-presentation

    J. Washio, T. Sato, K. Ikawa, N. Tanda, M. Iwakura, T. Koseki, N. Takahashi

    International Symposium for Interface Oral Health Science　2005/02/03

  163. Development of a portable bad-breath monitor and application for field study of halitosis. International-presentation

    N. Tanda, M. Iwakura, K. Ikawa, J. Washio, A. Kusano, T. Koseki

    International Symposium for Interface Oral Health Science　2005/02/03

  164. Nested PCR for detection of mutans streptococci in human dental plaque. International-presentation

    J. Matsuyama, T. Sato, G. Mayanagi, J. Washio, M. Yamaura, Y. Ito, K. Hashimoto, K. Nakajo, K. Kato, N. Takahashi

    International Symposium for Interface Oral Health Science　2005/02/03

  165. LiNbO3を用いた中赤外波長レーザーの生物学的効果

    鈴木麻衣子, 鷲尾純平, 小関健由

    東北大学歯学会　2004/06/22

  166. 鈴木麻衣子，鷲尾純平，小関健由，伊藤弘昌，三浦賀一

    細菌, 培養細胞への中赤外線波長レーザー照射による生物学的効果

    第１回東北大学バイオサイエンスシンポジウム　2004/05/14

  167. Hydrogen sulfide-producing bacteria in tongue coating and oral malodor. International-presentation

    J. Washio, T. Sato, T. Koseki, N. Takahashi

    第82回International Association for Dental Research　2004/03/12

  168. A field study for halitosis using new VSC monitor. International-presentation

    M. Iwakura, K. Ikawa, J. Washio, N. Tanda, A. Kusano, K. Suzuki, T. Koseki

    第82回International Association for Dental Research　2004/03/12

  169. 口臭と舌苔中の総菌数および硫化水素産生菌数との関係.

    鷲尾純平, 佐藤拓一, 井川恭子, 丹田奈緒子, 岩倉政城, 小関健由, 高橋信博

    第52回口腔衛生学会・総会　2003/09/26

  170. 地域活動体験を経た歯科衛生士の業務進展

    大沼禎子, 安斉深雪, 伊東美香, 折越文子, 片平玲子, 鴨原ヨシ子, 渡辺淳子, 朝倉みさ子, 鷲尾純平, 小関健由, 岩倉政城

    第15回日本口腔衛生学会東北地方会　2003/05/24

  171. 住民歯科保健学習組織構成員の助言を契機に始めた歯周病療養の体験報告

    広田好子, 朝倉みさ子, 鴨原ヨシ子, 鷲尾純平, 小関健由, 岩倉政城

    第15回日本口腔衛生学会東北地方会　2003/05/24

  172. 歯垢中のmutans streptococciのPCR法による検出について.

    熊谷 崇, 重光竜二, 太郎丸 毅, 鷲尾純平, 小関健由, 佐藤拓一, 高橋信博

    第42回東北大学歯学会　2002/12/13

  173. Development of "Breathtron", a bad-breath monitor for chair-side use. International-presentation

    M. Iwakura, K. Suzuki, H. Hariu, J. Washio, M. Shimura, K. Ikawa, N. Tanda

    日中医学大会2002　2002/11/03

  174. 舌苔細菌叢中の硫化水素産生菌の検出法に関する研究

    鷲尾純平, 佐藤拓一, 高橋信博

    第17回口腔嫌気性菌研究会　2002/10/03

  175. 某高等専門学校男子学生の1975年から2000年のウ蝕有病状況の変化

    井川恭子, 鷲尾純平, 楠本雅子, 松坂朋典, 針生ひろみ, 志村匡代, 丹田奈緒子, 千葉潤子, 小澤雄樹, 田浦勝彦

    第51回日本口腔衛生学会・総会　2002/09/14

  176. 第一線臨床に向けた口臭測定器の開発

    岩倉政城, 針生ひろみ, 鷲尾純平, 志村匡代, 井川恭子, 丹田奈緒子, 鈴木健吾

    第51回日本口腔衛生学会・総会　2002/09/14

  177. 口臭測定器ブレストロンの開発

    針生ひろみ, 志村匡代, 鷲尾純平, 井川恭子, 丹田奈緒子, 岩倉政城, 鈴木健吾

    第41回東北大学歯学会　2002/06/26

  178. 東北大学予防歯科口臭外来20年間の受診動態

    鷲尾純平, 針生ひろみ, 志村匡代, 井川恭子, 丹田奈緒子, 岩倉政城

    第14回日本口腔衛生学会東北地方会　2002/05/25

  179. 高専生におけるう蝕有病状況とフッ化物配合歯磨剤の使用状況に関する検討

    井川恭子, 田浦勝彦, 針生ひろみ, 志村匡代, 丹田奈緒子, 松坂朋典, 鷲尾純平

    第14回日本口腔衛生学会東北地方会　2002/05/25

  180. ZnO厚膜型センサの口臭測定器への応用

    鈴木健吾, 鷲尾純平, 岩倉政城

    第34回化学センサ研究発表会　2002/04/01

  181. 予防歯科口臭外来開設以来19年間の受診動態とその臨床統計

    針生ひろみ, 岩倉政城, 鷲尾純平, 志村匡代, 井川恭子, 丹田奈緒子, 坂本征三郎

    第40回東北大学歯学会　2001/12/14

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Research Projects 34

  1. セルフパワードウェアラブル口腔内情報モニタリングシステムの開発

    依田 信裕, 土方 亘, 伊藤 浩之, 柳田 保子, 鷲尾 純平, 佐々木 啓一, 日原 大貴

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2025/04/01 - 2028/03/31

  2. 口腔マイクロバイオームの新・存在意義～全身・口腔健康に寄与する亜硝酸産生の全容～

    鷲尾 純平, 高橋 信博, 安彦 友希, 互野 亮, 佐藤 拓一, 柳田 保子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2024/04/01 - 2028/03/31

  3. Microbial profiling of non-woven fabric masks and tablet-screens: Exploring the possibility of microbial transmission

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Niigata University

    2024/04 - 2027/03

  4. 口腔細菌叢で脳をまもる：抗認知症プラズマローゲンを産生する嫌気性菌の同定と応用

    高橋 信博, 鷲尾 純平, 仲川 清隆

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2023/06/30 - 2026/03/31

  5. 口腔マイクロバイオームの新機能：「副代謝系」の解明による口腔と全身の健康増進戦略

    高橋 信博, 佐藤 拓一, 鷲尾 純平, 坂本 光央

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2024/04/01 - 2025/03/31

  6. 呼気オミックスを用いた乳がん口腔支援システムの開発

    丹田 奈緒子, 石田 孝宣, 赤池 孝章, 鷲尾 純平, 多田 寛, 高橋 信博, 小関 健由

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

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    乳癌は世界で最も罹患率の高い悪性腫瘍で骨転移を伴うことが多く長期的な病状の制御と薬物療法が必要とされている。骨転移に伴う骨関連事象の抑制のため骨吸収抑制薬が使用されるが、その副作用である顎骨壊死に対し日本乳癌学会では薬剤使用開始前の口腔内リスク評価と薬剤使用中の定期的な口腔管理を推奨している。長期的な薬物療法中の乳癌患者の口腔管理には骨転移のリスクを含めた病状の理解が必要となる。２型アルデヒド脱水素酵素（aldehyde dehydrogenase 2: ALDH2）のALDH２遺伝子多型はアセトアルデヒドの体内蓄積を引き起こす。本研究の目的は、アルデヒド等代謝物の蓄積が骨転移の予測因子となりうるかについて呼気オミックスとALDH２遺伝子多型から解析し、薬物療法時ならびに骨吸収抑制薬使用時の口腔環境因子について探索することにより、長期薬物療法中乳癌患者の顎骨壊死等有害事象の予防を目指した口腔支援システムを開発することである。 初年度は「人を対象とする生命科学・医学系研究に関する倫理指針」に従い、東北大学大学院歯学研究科研究倫理委員会へ臨床研究倫理申請を行った。「乳癌患者の骨転移・骨関連事象に関する呼気・口腔環境の解析」という課題名のもと、研究実施許可を得た。その後当院総合外科（乳腺・内分泌外科）患者を対象に、現在まで30名の適格症例の研究登録同意を得た。飲酒・喫煙・口腔衛生についての質問とともに口腔内診査を行い、呼気凝縮液の回収、その際の安静時唾液採取、遺伝子検査等を進めている。

  7. フッ化物抵抗性根面齲蝕関連菌の代謝動態解明のためのトランスクリプトーム解析

    川嶋 順子, 安彦 友希, 鷲尾 純平

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2022/04/01 - 2025/03/31

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    口腔Actinomyces は、ヒトのプラーク細菌において口腔Streptococcus に次いで多く、根面齲蝕病巣からも高頻度で検出されることから、根面齲蝕との関連が注目されている。齲蝕は、主に口腔細菌の糖代謝によって産生される酸に起因するが、Actinomycesの糖代謝経路は代表的な齲蝕関連菌であるStreptococcusとは大きく異なる。Actinomycesは、齲蝕予防剤として世界中で広く利用されているフッ化物に耐性を持つことが明らかとなっているものの、その代謝経路の詳細は不明である。本研究では、口腔Actinomycesの代謝活性に対するフッ化物の効果に着目し、増殖抑制や酸産生抑制作用についてトランスクリプトーム解析による代謝酵素の遺伝子発現量の評価を中心とした代謝メカニズムに関する分析化学的および分子生物学的アプローチによって多角的に解析し、代謝経路の解明と効果的なフッ化物応用法を検討・提案することを目的としたものである。 トランスクリプトーム解析を行うため、今年度はRNA抽出の方法について主に検討を行った。RNA抽出の方法には主にビーズにより機械的破砕、酵素による破砕があり、対象とする細菌の種類・懸濁液の種類によって選択する必要がある。酵素法については、細菌によって最適な種類と濃度があり、今後の実験計画の拡張性を考えた場合には現実的ではないと判断した。また、今年度予定していたフッ化物による代謝動態変化の評価のためのサンプル収集については時間的な制約があり来年度行うこととした。

  8. 口腔マイクロバイオームの新機能：「副代謝系」の解明による口腔と全身の健康増進戦略

    高橋 信博, 佐藤 拓一, 鷲尾 純平, 坂本 光央

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2021/04/01 - 2025/03/31

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    近年、口腔マイクロバイオームと全身の健康との関連について多くの知見が集まりつつある。その関連をもたらす因子の一つとして、口腔マイクロバイオームが持つ、主要代謝系とは異なった「副代謝系」の代謝産物が推測されている。そこで本研究では、口腔マイクロバイオームに特異的で、口腔のみならず、「高血圧、がん、老化」等の全身の健康状態に関わる「アルコール代謝、硝酸塩代謝、ポリアミン代謝」といった副代謝系を明らかにし、さらに口腔マイクロバイオームにおける副代謝系を持つ細菌の割合や活性の程度と口腔・全身の健康状態との関連を探り、口腔マイクロバイオーム副代謝系の活用による口腔・全身の健康増進を目指すこととした。 初年度（2021年度）は、「高血圧、がん」にそれぞれ関連する「アルコール代謝、硝酸塩代謝」に着目し、口腔マイクロバイオームとしてヒト・プラークを用いてアルコール代謝として「エタノールからのアセトアルデヒド産生」、硝酸塩代謝として「硝酸塩からの亜硝酸産生」を指標として、当該活性を持つ細菌を分離同定した。その結果、前者ではVeillonella、Actinomyces、Neisseria、Streptococcusなどが、後者ではStreptococcus、Neisseriaなどが主体であることが明らかになった。細菌株毎に活性の有無が異なることから、必ずしもそれぞれの代謝活性を持つ細菌を細菌種レベルで分類できるわけではないことも分かった。さらに、それぞれの代謝経路の推定を行ったところ、アルコール代謝ではアルコール脱水素酵素が、硝酸塩代謝では硝酸還元酵素が責任酵素であることが分かった。但し、酸素濃度やpHに対する感受性はそれぞれ異なっていることも明らかになった。

  9. 『次代歯周病予防薬』開発のための『選択的予防メカニズム』の解明

    土門 ひと美, 鷲尾 純平, 高橋 信博

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2021/04/01 - 2024/03/31

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    Porphyromonas gingivalis(Pg)、Prevotella intermedia、Fusobacterium nucleatumといった歯周病関連菌は、タンパク質やジペプチドを特異的に代謝し増殖する。現在、歯周病の予防・治療薬は、クロルヘキシジンなどの非特異的殺菌剤が主流である。しかし、これら細菌のタンパク質・ジペプチド代謝を選択的に阻害する薬剤を見い出せれば、歯周病を『選択的に予防』でき『口腔マイクロバイオームの健全化』を図ることが可能となる。 令和３年度は、フッ化物によって阻害される新たな酵素の特定を試みた。令和元年度に、Pg ATCC33277Tの最終代謝産物および菌体内中間代謝物の量の変化を、それぞれHPLCおよびCE-TOFMSを用いて測定した。フッ化物の共存により、細菌数当たりの最終代謝産物量がいくつか増加していると共に、その代謝経路上にある幾つかの中間代謝物の減少がみられた。これらのことから、減少している中間体産物が他の代謝経路に流れていることが想定された。そこで、他の代謝経路に流れていると想定される反応系の中間代謝物を基質とし、超音波粉砕により菌体から抽出した酵素を用いて、フッ化物による酵素活性の影響を検討した。複数の酵素がフッ化物阻害対象として特定されつつあり、現在、阻害効果の最終確定を進めているところである。

  10. Relationship between breast milk & artificial formula and neonatal oral microbiota: Metagenomic and metabolomic analyses

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Niigata University

    2021/04/01 - 2024/03/31

  11. 新視点で探る口腔バイオフィルム細菌叢の存在意義～硝酸還元活性と全身・口腔健康～

    鷲尾 純平, 佐藤 拓一, 高橋 信博, 互野 亮

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2020/04 - 2024/03

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    口腔バイオフィルムは、これまでう蝕、歯周病、誤嚥性肺炎等の様々な病原因子としての“負”の作用がクローズアップされてきた。一方、構成細菌の持する硝酸還元活性により産生される亜硝酸塩は、血液循環の改善や、一部の口腔細菌の増殖を抑制することが近年示され、我々の健康維持に寄与しうるという“正”の作用を持つ可能性に注目が集まっている。 そこで私たちはこれまで、口腔バイオフィルム中の硝酸還元活性を持つ菌について網羅的探索を行い、口腔内における亜硝酸産生を担う細菌種として、Actinomyces, Shaalia, Veillonella, Neisseria, Rothia属がその大半を占めることを明らかにしてきた。 さらに、前述の細菌種のうち、いくつかを用いて、酸素濃度、pH、亜硝酸濃度、糖濃度、乳酸濃度等、種々の環境因子を変動させた際の硝酸還元活性の変化を測定した。その結果、いずれの菌においても、環境条件により、その産生能を大きく変動させることが明らかとなり、その変動は菌種によって傾向が異なることが示唆された。 本年度は、引き続きどのような条件下で最も効率的に亜硝酸が産生されるのかについて検討するとともに、亜硝酸をさらに分解する菌の探索にも着手し、いくつかの菌種で分解活性を持つことを認めた。なお、当初予定していた実際のプラーク試料をもちいた検討は、コロナの感染対策の影響により、少し計画を順延した。 次年度も引き続きこれらの点について明らかにしながら、研究を進める予定である。また、研究内容の公表に向けても取り組みたい。

  12. Study for maiking process of academia leading expand indications of Microscale Mist Plaque Removal Device

    Hihara Hiroki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2020/04/01 - 2023/03/31

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    This study evaluated the effectiveness and safety of the Micro Scale Mist spray UNIT (MSM-UNIT) in vitro. First, we made artificial biofilm model on simulated oral mucosa. Effectiveness was evaluated by biofilm removal rates. Biofilm removal rates was calculated from banalization images. Safety was evaluated surface roughness from SEM image. The biofilm removal rate of the MSM-UNIT was significantly higher than that of conventional device. The surface roughness of conventional device treated area was significantly larger than that of the MSM-UNIT. The MSM-UNIT could be used safely and effectively for biofilm removal on oral mucosa.

  13. Elucidation of root caries mechanism and consideration of prevention/treatment methods by comprehensive model of demineralization and proteolysis

    MAYANAGI Gen

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2019/04/01 - 2022/03/31

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    We have developed methods to visualize the distribution of proteolytic enzyme activity in the root tissues and its activation, and investigated the distribution and activation of enzyme activity and the effects of inhibitors on them. As a result, it was proved that the distribution of proteolytic enzyme activity in the root tissue and the enzymes are activated by acid. It was also found that proteolytic enzyme activity was inhibited by chlorhexidine, silver diamine fluoride, epigallocatechin gallate, and calcium hydroxide.

  14. Do Bacteria Cause Cancer? Elucidation of "cell-microbiome interaction" using metabolism as an indicator

    Takahashi Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2018/06/29 - 2021/03/31

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    It is becoming clear that bacteria are involved in the canceration of cells. Therefore, in this study, we examined the effect of bacteria on cells using metabolism as an indicator using "real-time metabolism monitoring system" that we developed. As a result, the following were clarified. It was found that (1) Porphromonas gingivalis (Pg), which is the most representative periodontal disease-associated bacterium among oral bacteria, suppresses cellular glucose metabolism, (2) the cell glucose metabolism inhibitor is present in the culture supernatant of Pg, and (3) it is not a Pg-specific proteolytic enzyme gingipain but a completely different protein.

  15. Development of predictive oral supportive system for perioperative breast cancer patients

    Tanda Naoko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2018/04/01 - 2021/03/31

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    Bone is the most frequent metastatic site of breast cancer, and metastases cause a poor quality of life due to bone pain, pathological fracture, hypercalcemia, and spinal cord compression. Bone metastases in breast cancer patients are a common concern for medical doctors and dentists. Bone-modifying agents, which are necessary to prevent skeletal-related events, are associated with osteonecrosis of the jaw as an adverse side effect. Prognostic risk factors of bone metastases are important for predictive oral supportive system for breast cancer patients. Univariate analysis and multivariate analysis were performed using the Cox proportional hazards model to investigate associations between prognostic variables and time to bone metastasis in primary operable breast cancer patients. In multivariate analysis, tumor stage and alcohol sensitivity were significant prognostic risk factors for bone metastases.

  16. What is a healthy oral microbiome? Proposal of oral health indicators based on metabolic resilience

    Takahashi Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2017/04/01 - 2021/03/31

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    1. The "metabolic database of oral microbiome" was expanded by newly discovering glucose metabolism of new caries-associated bacteria Bifidobacaterium and Scardvia, nitrate metabolism of Veillonella and Neisseria, and ethanol metabolism system of Streptococcus and Neisseria. 2. These new caries-related bacteria mainly produced acetic acid from sugars, and their metabolism showed high resistance to fluoride. 3. Furthermore, nitrate metabolism was biochemically coupled with lactic acid metabolism (lactic acid consumption), and the consumption of lactic acid improves the caries-induced environment, and nitrite, which is a nitrate metabolite, has an antibacterial effect. It has been clarified that multiple metabolic systems in the microbiome are coordinated with each other to bring about metabolic resilience.

  17. Structure and functional potential of intracanal microbiome: Bioinformatics for endodontic therapy

    Yamaki Keiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2017/04/01 - 2020/03/31

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    Microbial composition of infected root canals varies consistently among reports. To determine the characteristics of the endodontic microbiome, the intracanal flora of apical periodontitis in 13 patients were profiled and compared with the profiles of their saliva and plaque. Infected root canal wall dentin was harvested from the involved teeth and their saliva and plaque were collected. Bacterial DNA was extracted from the samples and 16S rRNA gene analysis targeting the V3-V4 region was conducted on the Illumina MiSeq platform using QIIME2. The functional potential of the microbiomes was inferred using PICRUSt2. The endodontic microbiomes were more anaerobic, rich in Firmicutes, and scarce in Actinobacteriota and Proteobacteria, compared with saliva and plaque microbiomes. Despite their difference in structure and membership, the nine most abundant metabolic pathways were common among them. The intra-individual variance in endodontic microbiome was also found.

  18. How is the nitrite contributing to our general and oral health promotion produced in oral microflora?

    WASHIO JUMPEI

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2017/04/01 - 2020/03/31

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    In recent years, it attracts attention that oral bacteria can metabolize nitrate included in yellow-green vegetables or saliva to nitrite, that has antibacterial activity and vasodilatation effect, and which may contribute to maintain our general and oral health.However, the details about the production of nitrite in the oral cavity is still unknown. Therefore, we tried to clarify the whole aspect of nitrite production in the oral cavity. The nitrite-producing activity in the oral cavity was higher in dental plaque than tongue coatings. The interindividual differences was also higher. It was clarified that the bacterial species such as Actinomyces, Shaalia, and Veillonella species known as commensal bacteria mainly contribute to the nitrite production in oral cavity. In addition, it was also clarified that those nitrite-producing activity can be fluctuated by the various environmental factors such as an oxygen concentration, pH and the lactate concentration.

  19. Profiling microbiota of bronchial fluids in the elderly and its association with oral cavities

    Sato Takuichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Niigata University

    2017/04/01 - 2020/03/31

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    The source of the bacteria involved in silent aspiration remains to be completely defined. The purpose of the present study was to obtain reliable evidence on silent aspiration of oral bacteria in elderly patients. Our results suggest that bacteria in bronchial fluids associated with silent aspiration are derived from saliva, and that the bronchial fluids of elderly patients with an impaired swallowing reflex may have a characteristic microbiota. In addition, our results suggest that oral hygiene is important to reduce silent aspiration and to subsequently prevent the occurrence of aspiration pneumonia in elderly patients.

  20. Developing the model using pH/calcium fluorescent imaging for prevention and treatment of dental caries

    MAYANAGI Gen

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2016/04/01 - 2020/03/31

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    This study aimed to develop the methods to assess pH change at the interface between material or tooth and bacteria continuously under the same kind of conditions as an oral microenvironment in vitro. The fluoride releasing materials and the silver diamine fluoride coated tooth inhibited pH fall at the interface between material/tooth and bacteria. The degree of inhibition of pH fall seemed to correspond to the amounts of fluoride and silver detected, suggesting that the fluoride and silver released from these materials inhibited pH fall.

  21. Comprehensive analysis in oral microbiome as a promoting factor for esophageal cancer

    TANDA NAOKO, ITO Emi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    Acetaldehyde is a powerful carcinogen in thoracic esophageal cancer. The aim of this study is to evaluate perioperative change of mouth acetaldehyde and its causal oral bacteria in thoracic esophageal cancer patients under intensive oral care. Analysis was done compared to control cases. Drinking and smoking histories were longer and oral condition was worse in patients group than in control. Acetaldehyde in mouth air of thoracic esophageal cancer patients decreased after oral intensive care before operation. It also decreased after operation. It is suggested that intensive oral care for perioperative thoracic esophageal cancer patients decreases acetaldehyde produced in oral microbiome.

  22. Carbohydrate metabolism related to cariogenicity of oral Bifidobacterium

    ABIKO YUKI, MANOME Ayumi, KAMEDA Mai, KAWASHIMA Junko, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    Bifidobacterium have recently been detected in carious lesions. Thus, the purpose was to examine the cariogenic potential of oral Bifidobacterium by analyzing the carbohydrate metabolism and acidic end-products. In addition, intracellular polysaccharide was characterized. The acid production of Bifidobacterium was lower than that of S. mutans, and maintained even after 2 hours. Without glucose addition, the acid production was also found. Both with and without glucose, the acid production was higher at pH 5.5 than that at pH 7.0. The main acidic end-product was acetate. Absorption spectrum indicated that the intracellular polysaccharide was glycogen-like. Bifidobacterium showed a higher acid production at pH 5.5 than 7.0, and had a considerable amount of glycogen-like intracellular polysaccharides, suggesting that oral Bifidobacterium can maintain and promote the environment of carious lesions, i.e. sugar limit and acidic.

  23. The elucidation of the pathogenic activity of periodontal disease-related bacteria by metabolomics approaches; The establishment of new method to monitor their metabolic activity in real-time

    Washio Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04/01 - 2018/03/31

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    It has been suggested that the periodontal disease-related bacterial "metabolism" was strongly related to the pathogenicity of periodontal disease. However, they were still unclear because the study about their metabolism was not done enough by technical limitation. Therefore, in this KAKENHI project, I established the new method to monitor their metabolic activity easily in real-time and improved the technical method for the metabolome analysis of periodontal disease-related bacteria. The results in this study suggested that their metabolism was affected greatly by the environmental factors and drugs in actual. These new methods and knowledges could contribute to elucidate the onset mechanism of periodontal diseases and the development of new strategy for their treatment and prevention.

  24. Metabolome analysis of oral biofilm: a possible risk assessment of oral diseases using oral microbiome metabolism

    TAKAHASHI Nobuhiro

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    This study aimed to clarify the whole picture of metabolic systems of oral biofilm through the metabolome analysis and metabolic activity measurement, and to consider an assessment of oral health and disease using oral microbiome metabolism. The CE-MS metabolome system enabled to analyze the metabolites associated with carbohydrates and amino acids comprehensively and revealed the whole picture of metabolic systems of carbohydrates and amino acids in the oral biofilm. In addition, the metabolic activity of a tiny amount of oral biofilm was successfully measured using the alamarblue system. Furthermore, the metabolic activity was diverse among sample sites and metabolic substrates, suggesting that these metabolic characteristics of oral microbiome can be used as an indicator of oral health and disease.

  25. Multifaceted approach to the analyses of dental plaque biofilm targeting dental caries-associated bacteria, and its application to dental caries prevention

    Matsuyama Junko, SATO Takuichi, WASHIO Jumpei, TAKENAKA Shoji, OHSHIMA Hayato

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Niigata University

    2013/04/01 - 2016/03/31

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    As an animal model of dental caries, mice have been generally utilized. In this study, dental plaque microbiota of pre- and post-weanling, were analyzed by using molecular biological techniques. Enterococcus, Escherichia, Lactobacillus and Lactococcus were predominant. From the findings of the present study, the bacterial composition of dental plaque microbiota of mice varies markedly from that of humans (in which Streptococcus, Actinomyces and Veillonella are predominant); and this may be due to differences in the daily diet.

  26. Metabolomics of cancer tissue -challenge to the paradoxical energy metabolism of cancer cells

    TAKAHASHI Nobuhiro, TAKAHASHI Tetsu, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2013/04/01 - 2015/03/31

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    This study aimed to characterize the metabolic system of oral squamous cell carcinoma (OSCC) by metabolome analysis. The metabolome profiles were obtained from OSCC and its surrounding normal tissues, using CE-TOFMS. Enhancement of glucose consumption and lactate production (Warburg effect) was observed in OSCC tissues, while the downstream intermediates in the glycolysis were decreased and glutamine degradation (glutaminolysis) was enhanced . These results suggest that OSCC tissues supply metabolic intermediates from glucose for biosynthesis via the pentose phosphate cycle and obtain energy from glutamine with lactate production.

  27. The elucidation of the pathogenic activity of oral biofilm by Metabolomics approach

    WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2011/04/28 - 2015/03/31

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    This study aimed to elucidate, in vivo, the metabolism of several substrates in the oral biofilm and the actual influences of medicines such as fluoride and sugar substitutes such as xylitol to them through the metabolomic analysis approach using CE-TOFMS. Consequently, it showed that, as well as sugar, the amino acids, especially glutamine and glutamate, are actually used as substrates in the oral biofilm. Moreover, it suggested that each amino acid is fermented by its respectively characteristic metabolism. The study also showed that fluoride can influence the sugar metabolism, including the inhibition of enolase and acid production, in the real oral biofilm as well, and suggested that fluoride affects some other metabolic reactions. In contrast, xylitol was suggested to have almost no effect on acid production and the metabolome profile in the oral biofilm, although xylitol 5-phosphate was produced.

  28. Metabolomic approach to oral biofilm and saliva: possible risk indicator for oral diseases

    TAKAHASHI Nobuhiro, KOSEKI Takeyoshi, SENPUKU Hidenobu, SAKAMOATO Mitsuo, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2010/04/01 - 2014/03/31

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    Metabolome analysis for oral biofilm and saliva using CE-TOFMS was established. This method made it possible to analyze not only sugar metabolism but also amino acid metabolism in oral biofilm and even in saliva, where only small amount of metabolites (about 1/100 of oral biofilm) was contained. In addition, the analyses of the effect of sugar alcohols and fluorides on the biofilm metabolism suggested close coordination between sugar and amino acid metabolisms. Metabolome profiles of sugar and amino acid metabolisms can be used for risk indicators of dental caries and periodontitis/oral malodor, respectively.

  29. Metabolomic approach to metabolic biology of cancer cells

    TAKAHASHI Nobuhiro, ECHIGO Seishi, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

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    Metabolome analyses of oral squamous cancer (OSC) were performed by CE-TOFMS, targeting metabolites of Embden-Meyerhof-Parnas pathway (EMPP),pentose-phosphate pathway, TCA cycle and metabolic pathways for amino acids. Method for metabolome analyses of OSC was established. The results suggest that the enhancement of EMPP (Warburg effect) occurred in OSC, as previously reported for other cancers, and that amino acids such as glutamine were utilized as an additional energy source for OSC.

  30. Two dimensional evaluation by volatile gases for oral condition of diabetes mellitus

    TANDA Naoko, KOSEKI Takeyoshi, HINOKIO Yoshinori, TAKAHASHI Nobuhiro, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2008 - 2010

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    We studied acetone in exhaled air, volatile sulfur compounds and acetone in mouth air, to analyze relation between general condition and oral environment of patients with diabetes mellitus, with healthy volunteers as control. Acetone in exhaled and mouth air of type 2 diabetes patients decreased with improvement of plasma glucose during hospitalization. Study of healthy volunteers with good oral condition showed significantly strong correlation between acetone in exhaled air and acetoacetic acid or 3-hydroxybutyric acid in plasma after fasting overnight. There was no significant correlation between acetone and volatile sulfur compounds. This study suggests possibility that non-invasive acetone measuring indicates ketone bodies in plasma, which reflect pathologic condition of diabetes mellitus.

  31. Clarification of the acceleration mechanism of the production of oral malodor substances brought by the oral environmental factors and the interaction between various bacterial strains.

    WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2008 - 2010

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    The hydrogen sulfide(H_2S) is the main source of oral malodor. The production activity and the production mechanism of H_2S by the oral indigenous bacteria, Veillonella species and Actinomyces species, were examined. It was shown that these bacterial species have H_2S production activity and the activity was influenced by the environmental factors such as pH and the lactate concentration. Moreover, the mechanisms were slightly different depending on the bacterial strain even within same belonging. There has actually been suggested that there is possibility of H_2S production activity being influenced by its coexistence with other saccharolytic bacteria, therefore it is necessary to understand the cause of oral malodor in relation to bacteria not only as a single bacteria but also as the entire oral cavity including the oral biofilm microorganism and the oral environmental factors.

  32. 口臭原因への細菌学的・生化学的アプローチ〜科学的口臭予防への礎〜

    鷲尾 純平

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 若手研究(スタートアップ)

    Category: 若手研究(スタートアップ)

    Institution: 東北大学

    2006 - 2007

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    口臭の主な原因は口腔細菌の代謝産物によるものが大部分であると考えられるが、中でも口腔Veillonellaが口臭発生部位である舌苔中の主な硫化水素産生菌の一つであることを報告してきた(Washio J et al.,2005)。 Veillonella属は口腔常在細菌の一つであり、システインやシステイン含有ペプチドなどからH_2Sを産生することで口臭の一因を担うと考えられる。また、Veillonella属は乳酸をエネルギー源として利用する。乳酸はStreptococcus属などが糖を代謝することで産生され、口腔内に多く存在すると考えられ、これらの乳酸をVeillonellaが利用していると考えられている。従って実際に、Veillonellaがシステインを代謝する環境中には、同時に乳酸が多く存在することが考えられる。そこで、環境中の乳酸がVeillonella属のH_2S産生能に影響を及ぼすのか検討を行った。 Veillonella属によるH_2S産生量は酸性より中性付近の方が多くなったが、乳酸ナトリウム存在下ではH_2S産生量がさらに4.5〜23.7倍に増加した。また、粗酵素抽出液にシステインを加えるとH_2Sを産生し、その産生量はpH5よりもpH7の方が多かったが、乳酸ナトリウムはH_2S産生に影響しなかった。これらより、実際の口腔では、舌苔中のpHだけではなく乳酸濃度が、口臭レベルに影響する可能性が考えられた。この時、乳酸はVeillonella菌体内の酵素活性を直接活性化するのではなく、Veillonellaのシステイン取り込み系を促進する可能性が示唆された。

  33. 口腔微生物叢の「揺りかご」:舌苔バイオフィルムの生物学

    高橋 信博, 小関 健由, 佐藤 拓一, 鷲尾 純平, 今井 奨

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 萌芽研究

    Category: 萌芽研究

    Institution: 東北大学

    2005 - 2007

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    本年度は、昨年度に引き続き、口臭原因菌の一つと捉えられる口腔Veillonella属の硫化水素産生活性について検討し、(1)環境因子、とくに口腔内に多く存在する乳酸等の有機酸が硫化水素産生能に与える影響、及び、(2)硫化水素産生に関わる代謝酵素の検索を行った。培養したVeillonella atypica、 V. dispar、 V. parvulaの各標準株(ATCC株)の培養菌にシステインを加えて3時間インキュベートし、産生した硫化水素をメチレンブルー法で定量した。さらにこの反応系に乳酸等の各種有機酸を加え、硫化水素産生活性への影響を検討した。また、培養菌を超音波処理して無細胞抽出液を調整し、システインからの硫化水素産生に関わる酵素活性を検索した。Veillonellaのシステインからの硫化水素産生活性は、乳酸が存在すると著しく増加した。Veillonellaの無細胞抽出液は、システインから硫化水素を産生する酵素活性を示したが、無細胞抽出液に乳酸を添加しても活性の増加は認められなかった。以上のことから、Veillonellaは乳酸の存在下、システインから効率よく硫化水素を産生することが明らかになった。乳酸による促進作用は、酵素の活性化作用ではなく、システインの取込み促進であるものと予測された。口臭源の一つである硫化水素は口腔細菌によって産生されるが、その産生活性はpH(昨年度成果)や有機酸(今年度成果)という口腔環境によって影響を受けるものと考えられ、口腔環境のコントロールによって口臭を抑制し得るものと考えられる。

  34. Profiling of oral bacterial flora using DNA chips and its application to the risk evaluation of oral diseases of children.

    TAKAHASHI Nobuhiro, HATA Shinji, SATO Takuichi, MATSUYAMA Junko, NAKAJO Kazuko, WASHIO Jumpei

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2004 - 2006

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    Oral biofilms have been reported to be associated with oral infections such as dental caries and periodontitis. And also, oral biofilms are microbial ecosystems which contain various amounts and sorts of microorganisms, and thus controlling these microbial florae are thought to be one of the ways toward prevention of these oral infections. The aim of this study was to establish DNA micro arrays system to detect oral infections-associated bacteria utilizing species-specific DNA probes of 16S ribosomal RNA genes. Twenty dentinal endodontic lesions-associated bacteria including mutans streptococci and twenty-three periodontitis-associated bacteria including Porphyromonas gingivalis, Tannerella forsythia and Mogibacterium timidum were selected in this study, and the specificity and sensitivity of the DNA micro arrays were tested. In addition, we have been planning to compare the DNA micro arrays system with the direct-＆ real-time PCR on their specificities and sensitivities utilizing one-hundred subgingival plaque biofilms for the present.

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Teaching Experience 1

  1. 基礎研究実習　東北大学