TOHOKU UNIVERSITY Researchers - Narufumi Kitamura

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Narufumi Kitamura

Section

Graduate School of Medicine

Job title

Associate Professor

Degree

Ph.D in Engineering (Kyoto University)

researchmap

https://researchmap.jp/n-kitamura

J-GLOBAL ID

201801003506004974

e-Rad No.

50624912

Research History 6

2021/06 - Present

Graduate School of Medicine, Tohoku University　Department of Medical Physics　Associate Professor
2019/02 - 2021/05

Graduate School of Medicine, Tohoku University　Department of Medical Physics　Lecturer
2017/04 - 2019/01

Graduate School of Medicine, Tohoku University　Department of Medical Physics　Assistant Professor
2013/10 - 2017/03

Graduate School of Medicine, Tohoku University　Department of Surgical Oncology　Assistant Professor
2011/10 - 2013/10

Institute for Frontier Medical Sciences, Kyoto University　Department of Reparative Materials　Postdoctoral fellow
2006/04 - 2007/09

KASYU CO., LTD.

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Education 4

Kyoto University　Graduate School of Engineering　Department of Polymer Chemistry

2008/10 - 2011/09
Kyoto University　Graduate School of Engineering　Department of Chemical Engineering

2007/10 - 2008/09
Kyoto University　Graduate School of Engineering　Department of Chemical Engineering

2005/04 - 2006/03
Kyoto University　Faculty of Engineering　Undergraduate School of Industrial Chemistry

2000/04 - 2005/03

Professional Memberships 4

Society of Nano Science and Technology
THE JAPANESE CANCER ASSOCIATION
THE SOCIETY OF POLYMER SCIENCE, JAPAN
THE CHEMICAL SOCIETY OF JAPAN

Research Interests 5

cancer
radiotherapy
nanomaterial
biochemistry
biomaterial

Research Areas 5

Energy / Quantum beam science / Angiography, Radiotherapy
Life sciences / Radiology / Angiography, Radiotherapy
Other / Other / pathological diagnosis Laboratory medicine
Nanotechnology/Materials / Polymer chemistry / nanomaterial
Nanotechnology/Materials / Biochemistry / Imaging Agent, Sensitizer

Papers 32

Intracellular oxygen monitoring and oxygen demand assessment using Ir(ppy)3-encapsulated polymeric micelles. International-journal Peer-reviewed

Narufumi Kitamura, Keiji Kajiwara, Shoichi Yamamoto, Mayumi Takano-Kasuya, Kunimasa Hiyama, Hiroshi Kita, Kohsuke Gonda

Bioorganic & medicinal chemistry　137　118632-118632　2026/03/14

DOI： 10.1016/j.bmc.2026.118632 　

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Intracellular oxygen concentration is a pivotal indicator of cellular metabolic and functional states that may be influenced by pathologic conditions, including hypoxia in solid tumors and hyperglycemia. Currently, methods for quantifying cellular oxygen levels-particularly within immune cells implicated in hypoxic dysfunction-are underdeveloped. Here, we introduce a novel biocompatible intracellular oxygen sensor developed by encapsulating the poorly water-soluble phosphorescent dye tris(2-phenylpyridinato)iridium(III) (Ir(ppy)₃) within micelles formed from 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers. This micelle system enables efficient delivery and stable retention of the phosphorescent dye within the cytoplasm. Building on prior confocal microscopy studies characterizing the properties of MPC polymers, our findings reveal that cellular uptake of these polymers occurs via a cell-penetrating translocation mechanism, effectively bypassing significant endosomal sequestration and thus facilitating cytoplasmic oxygen sensing. Micelles prepared with a 30-μM Ir(ppy)₃ stock solution exhibited optimal phosphorescence and clear oxygen sensitivity. Using this sensor, we observed distinct oxygen consumption kinetics between KPL-4 and MDA-MB231 breast cancer cells. Moreover, analysis of the response of NK92-CD16 cells, an NK cell-derived immune cell line, to varying glucose levels revealed that high glucose conditions (300-400 mg/dL) significantly suppress the hypoxia-induced increase in phosphorescence, indicating reduced metabolic oxygen demand. Overall, this MPC micelle-encapsulated Ir(ppy)₃ platform serves as a robust tool for evaluating cellular metabolic states and in vitro responses to microenvironmental cues, such as hypoxia and hyperglycemia.
Microregional HER2/HER3 density on cancer cells based on multi-point binding detection using nanoparticle-modified cantilever AFM Peer-reviewed

Narufumi Kitamura, Daisuke Yamamoto, Mayumi Takano-Kasuya, Hiroshi Tada, Naoya Saito, Naoko Furusawa, Yasushi Nakano, Takanori Ishida, Kohsuke Gonda

Colloids and Surfaces B: Biointerfaces　256　115043-115043　2025/12
Publisher: Elsevier BV
DOI： 10.1016/j.colsurfb.2025.115043 　

ISSN： 0927-7765
Mitochondrial dynamics as a novel treatment strategy for triple-negative breast cancer. International-journal Peer-reviewed

Yuechen Wang, Narumi Harada-Shoji, Narufumi Kitamura, Yuto Yamazaki, Akiko Ebata, Masakazu Amari, Mika Watanabe, Minoru Miyashita, Hiroshi Tada, Takaaki Abe, Takashi Suzuki, Kohsuke Gonda, Takanori Ishida

Cancer medicine　13　(2)　e6987　2024/01

DOI： 10.1002/cam4.6987 　

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INTRODUCTION: Triple-negative breast cancer (TNBC), recognized as the most heterogeneous type of breast cancer (BC), exhibits a worse prognosis than other subtypes. Mitochondria dynamics play a vital role as mediators in tumorigenesis by adjusting to the cell microenvironments. However, the relationship between mitochondrial dynamics and metabophenotype exhibits discrepancies and divergence across various research and BC models. Therefore, this study aims to explore the role of mitochondrial dynamics in TNBC drug resistance and tumorigenesis. METHODS: The Wst-8 test was conducted to assess doxorubicin sensitivity in HCC38, MDA-MB-231 (TNBC), and MCF-7 (luminal). Confocal microscopy and FACS were used to quantify the mitochondrial membrane potential (ΔφM), mitophagy, and reactive oxygen species (ROS) production. Agilent Seahorse XF Analyzer was utilized to measure metabolic characteristics. Dynamin-related protein-1 (DRP1), Parkin, and p62 immunohistochemistry staining were performed using samples from 107 primary patients with BC before and after neoadjuvant chemotherapy (NAC). RESULTS: MDA-MB-231, a TNBC cell line with reduced sensitivity to doxorubicin, reduced ΔφM, and enhanced mitophagy to maintain ROS production through oxidative phosphorylation (OXPHOS)-based metabolism. HCC38, a doxorubicin-sensitive cell line, exhibited no alterations in ΔφM or mitophagy. However, it demonstrated an increase in ROS production and glycolysis. Clinicopathological studies revealed that pretreatment (before NAC) expression of DRP1 was significant in TNBC, as was pretreatment expression of Parkin in the hormone receptor-negative group. Furthermore, low p62 levels seem to be a risk factor for recurrence-free survival. CONCLUSION: Our findings indicated that the interplay between mitophagy, linked to a worse clinical prognosis, and OXPHOS metabolism promoted chemotherapy resistance in TNBC. Mitochondrial fission is prevalent in TNBC. These findings suggest that targeting the unique mitochondrial metabolism and dynamics in TNBC may offer a novel therapeutic strategy for patients with TNBC.
Clinical Significance of ABCG2/BCRP Quantified by Fluorescent Nanoparticles in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy Peer-reviewed

Hiroshi Tada, Kohsuke Gonda, Narufumi Kitamura, Takanori Ishida

Cancers　15　(8)　2365-2365　2023/04/18
Publisher: MDPI AG
DOI： 10.3390/cancers15082365 　

eISSN： 2072-6694

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Breast cancer resistance protein (BCRP), also known as ATP-binding cassette transporter G2 (ABCG2), is associated with chemotherapy resistance. BCRP is also implicated in breast cancer stem cells, and is reported as a poor prognostic factor. However, the relationship of BCRP levels in breast cancer tissues with chemotherapy resistance and prognosis has not been clarified. We aimed to evaluate the correlation between BCRP expression and prognosis in breast cancer using immunohistochemistry with fluorescent phosphor-integrated dots (IHC-PIDs). A total of 37 breast cancer patients with residual cancer in the primary tumor and axillary lymph nodes were evaluated. BCRP levels in breast cancer tissue and metastatic lymph nodes were quantitatively detected after neoadjuvant chemotherapy (NAC). Among these 37 patients, 24 had corresponding core needle biopsies obtained before NAC. Biomarker assay with IHC-PIDs showed high accuracy for the quantitative assessment of BCRP with low expression. High BCRP expression in the primary tumor and metastatic lymph nodes after preoperative chemotherapy was associated with worse overall survival. In conclusion, high BCRP levels may be associated with poor prognosis in patients with breast cancer, having residual tumors within the primary tumor and lymph nodes after preoperative chemotherapy. These findings provide a basis for further appropriate adjuvant therapy in these patients.
Development of gold-coated magnetic nanoparticles as a theranostic agent for magnetic hyperthermia and CT imaging applications Peer-reviewed

Loi Tonthat, Mone Kimura, Tomoyuki Ogawa, Narufumi Kitamura, Yoshio Kobayashi, Kohsuke Gonda, Shin Yabukami

AIP Advances　13　(2)　025239-025239　2023/02/01
Publisher: AIP Publishing
DOI： 10.1063/9.0000592 　

eISSN： 2158-3226

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In this study, we aim to develop gold-coated Fe₃O₄ nanoparticles (Fe₃O₄@Au NPs) as theranostic agents for magnetic hyperthermia and CT imaging applications. The Fe₃O₄ NPs were synthesized via thermal decomposition method, and the gold was then deposited onto the surface of Fe₃O₄ NPs by reducing gold acetate at 190 °C. The average sizes of Fe₃O₄ and Fe₃O₄@Au NPs were 5.2 nm and 6.1 nm, respectively, which are effectively removed by the kidneys. The magnetization of Fe₃O₄@Au NPs (9.7 emu/g-Fe₃O₄) at 300 K was much smaller than that of Fe₃O₄ NPs (52.4 emu/g-Fe₃O₄). The heating efficiency of Fe₃O₄@Au NPs in water was sufficient to treat the tumor at 43–45 °C, and their high CT value of 851 HU was obtained. The synthesized ultrasmall Fe₃O₄@Au NPs showed great promise as a potential theranostic agent for magnetic hyperthermia and CT imaging applications.
X-ray irradiation negatively affects immune responses in the lymphatic network Peer-reviewed

Sawa Kanabuchi, Narufumi Kitamura, Mayumi Takano-Kasuya, Tomoya Inose, Chihiro Nishidate, Mizuki Yamanashi, Makoto Kudo, Tatsuki Ito, Naho Ito, Hiroshi Okamoto, Yusuke Taniyama, Yoshio Kobayashi, Takashi Kamei, Kohsuke Gonda

Microvascular Research　104511-104511　2023/02
Publisher: Elsevier BV
DOI： 10.1016/j.mvr.2023.104511 　

ISSN： 0026-2862
Development of X-ray contrast agents using single nanometer-sized gold nanoparticles and lactoferrin complex and their application in vascular imaging Peer-reviewed

Tomoya Inose, Narufumi Kitamura, Mayumi Takano-Kasuya, Masayuki Tokunaga, Norikazu Une, Chihiro Kato, Mayu Tayama, Yukina Kobayashi, Noriko Yamauchi, Daisuke Nagao, Takuji Aimiya, Naoko Furusawa, Yasushi Nakano, Yoshio Kobayashi, Kohsuke Gonda

Colloids and Surfaces B: Biointerfaces　203　111732-111732　2021/07
Publisher: Elsevier BV
DOI： 10.1016/j.colsurfb.2021.111732 　

ISSN： 0927-7765
The anti-angiogenic agent lenvatinib induces tumor vessel normalization and enhances radiosensitivity in hepatocellular tumors International-journal Peer-reviewed

Norikazu Une, Mayumi Takano-Kasuya, Narufumi Kitamura, Mineto Ohta, Tomoya Inose, Chihiro Kato, Ryuichi Nishimura, Hiroshi Tada, Shigehito Miyagi, Takanori Ishida, Michiaki Unno, Takashi Kamei, Kohsuke Gonda

Medical Oncology　38　(6)　60-60　2021/06
Publisher: Springer Science and Business Media LLC
DOI： 10.1007/s12032-021-01503-z 　

ISSN： 1357-0560

eISSN： 1559-131X

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The evaluation of angiogenesis inhibitors requires the analysis of the precise structure and function of tumor vessels. The anti-angiogenic agents lenvatinib and sorafenib are multi-target tyrosine kinase inhibitors that have been approved for the treatment of hepatocellular carcinoma (HCC). However, the different effects on tumor vasculature between lenvatinib and sorafenib are not well understood. In this study, we analyzed the effects of both drugs on vascular structure and function, including vascular normalization, and investigated whether the normalization had a positive effect on a combination therapy with the drugs and radiation using micro X-ray computed tomography with gold nanoparticles as a contrast agent, as well as immunohistochemical analysis and interstitial fluid pressure (IFP) measurement. In mice subcutaneously transplanted with mouse HCC cells, treatment with lenvatinib or sorafenib for 14 days inhibited tumor growth and reduced the tumor vessel volume density. However, analysis of integrated data on vessel density, rates of pericyte-covering and perfused vessels, tumor hypoxia, and IFP measured 4 days after drug treatment showed that treatment with 3 mg/kg of lenvatinib significantly reduced the microvessel density and normalized tumor vessels compared to treatment with 50 mg/kg of sorafenib. These results showed that lenvatinib induced vascular normalization and improved the intratumoral microenvironment in HCC tumors earlier and more effectively than sorafenib. Moreover, such changes increased the radiosensitivity of tumors and enhanced the effect of lenvatinib and radiation combination therapy, suggesting that this combination therapy is a powerful potential application against HCC.
Heterogeneous Drug Efficacy of an Antibody-Drug Conjugate Visualized Using Simultaneous Imaging of Its Delivery and Intracellular Damage in Living Tumor Tissues Peer-reviewed

Kohsuke Gonda, Hiroshi Negishi, Mayumi Takano-Kasuya, Narufumi Kitamura, Naoko Furusawa, Yasushi Nakano, Yoh Hamada, Masayuki Tokunaga, Hideo Higuchi, Hiroshi Tada, Takanori Ishida

TRANSLATIONAL ONCOLOGY　13　(6)　100764-100764　2020/06

DOI： 10.1016/j.tranon.2020.100764 　

ISSN： 1936-5233
A millisecond structured illumination microscope for super-resolution live cell imaging Peer-reviewed

Tomu Suzuki, Shinji Kajimoto, Narufumi Kitamura, Mayumi Takano-Kasuya, Naoko Furusawa, Yasushi Nakano, Hiroshi Fukumura, Kohsuke Gonda, Takakazu Nakabayashi

Applied Physics Express　13　(4)　045002 (4 page)　2020/03

DOI： 10.35848/1882-0786/ab7cef 　
Automated Quantification of Extranuclear ERα using Phosphor-integrated Dots for Predicting Endocrine Therapy Resistance in HR+/HER2- Breast Cancer. International-journal Peer-reviewed

Zhaorong Guo, Hiroshi Tada, Narufumi Kitamura, Yoh Hamada, Minoru Miyashita, Narumi Harada-Shoji, Akiko Sato, Yohei Hamanaka, Kouki Tsuboi, Nobuhisa Harada, Mayumi Takano-Kasuya, Hisatake Okada, Yasushi Nakano, Noriaki Ohuchi, Shin-Ichi Hayashi, Takanori Ishida, Kohsuke Gonda

Cancers　11　(4)　2019/04/12

DOI： 10.3390/cancers11040526 　

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In addition to genomic signaling, Estrogen receptor alpha (ERα) is associated with cell proliferation and survival through extranuclear signaling contributing to endocrine therapy (ET) resistance. However, the relationship between extranuclear ERα and ET resistance has not been extensively studied. We sought to measure extranuclear ERα expression by immunohistochemistry using phosphor-integrated dots (IHC-PIDs) and to assess its predictive value for ET resistance. After quantitative detection of ERα by IHC-PIDs in vitro, we developed "the nearest-neighbor method" to calculate the extranuclear ERα. Furthermore, tissue sections from 65 patients with HR+/HER2- BC were examined by IHC-PIDs, and the total ERα, nuclear ERα, extranuclear ERα PIDs score, and ratio of extranuclear-to-nuclear ERα (ENR) were measured using the novel method. We demonstrate that quantification of ERα using IHC-PIDs exhibited strong correlations to real-time qRT-PCR (r2 = 0.94) and flow cytometry (r2 = 0.98). High ERα ENR was significantly associated with poor overall survival (p = 0.048) and disease-free survival (DFS) (p = 0.007). Multivariate analysis revealed that the ERα ENR was an independent prognostic factor for DFS [hazard ratio, 3.8; 95% CI, 1.4-11.8; p = 0.006]. Our automated measurement has high accuracy to localize and assess extranuclear ERα. A high ERα ENR in HR+/HER2- BC indicates decreased likelihood of benefiting from ET.
Quantitative analyses of amount and localization of radiosensitizer gold nanoparticles interacting with cancer cells to optimize radiation therapy Peer-reviewed

Keiichiro Hatoyama, Narufumi Kitamura, Mayumi Takano-Kasuya, Masayuki Tokunaga, Takahiro Oikawa, Mineto Ohta, Yoh Hamada, Hiroshi Tada, Yoshio Kobayashi, Takashi Kamei, Kohsuke Gonda

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　508　(4)　1093-1100　2019/01

DOI： 10.1016/j.bbrc.2018.12.016 　

ISSN： 0006-291X

eISSN： 1090-2104
Quantitative diagnosis of HER2 protein expressing breast cancer by single-particle quantum dot imaging Peer-reviewed

Minoru Miyashita, Kohsuke Gonda, Hiroshi Tada, Mika Watanabe, Narufumi Kitamura, Takashi Kamei, Hironobu Sasano, Takanori Ishida, Noriaki Ohuchi

CANCER MEDICINE　5　(10)　2813-2824　2016/10

DOI： 10.1002/cam4.898 　

ISSN： 2045-7634
X-ray computed tomography imaging of a tumor with high sensitivity using gold nanoparticles conjugated to a cancer-specific antibody via polyethylene glycol chains on their surface Peer-reviewed

Tomohiko Nakagawa, Kohsuke Gonda, Takashi Kamei, Liman Cong, Yoh Hamada, Narufumi Kitamura, Hiroshi Tada, Takanori Ishida, Takuji Aimiya, Naoko Furusawa, Yasushi Nakano, Noriaki Ohuchi

SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS　17　(1)　387-397　2016

DOI： 10.1080/14686996.2016.1194167 　

ISSN： 1468-6996

eISSN： 1878-5514
Interaction between cells and poly(ethylene glycol)-lipid conjugates Peer-reviewed

Toru Itagaki, Yusuke Arima, Rei Kuwabara, Narufumi Kitamura, Hiroo Iwata

COLLOIDS AND SURFACES B-BIOINTERFACES　135　765-773　2015/11

DOI： 10.1016/j.colsurfb.2015.08.014 　

ISSN： 0927-7765

eISSN： 1873-4367
A DNA hybridization system for labeling of neural stem cells with SPIO nanoparticles for MRI monitoring post-transplantation Peer-reviewed

Edgar Y. Egawa, Narufumi Kitamura, Ryusuke Nakai, Yusuke Arima, Hiroo Iwata

BIOMATERIALS　54　158-167　2015/06

DOI： 10.1016/j.biomaterials.2015.03.017 　

ISSN： 0142-9612

eISSN： 1878-5905
Highly Sensitive Imaging of Cancer with Functional Nanoparticles Peer-reviewed

Kohsuke Gonda, Yoh Hamada, Narufumi Kitamura, Hiroshi Tada, Minoru Miyashita, Takashi Kamei, Takanori Ishida, Noriaki Ohuchi

JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY　28　(6)　731-736　2015

DOI： 10.2494/photopolymer.28.731 　

ISSN： 0914-9244
Labeling of islet cells with iron oxide nanoparticles through DNA hybridization for highly sensitive detection by MRI Peer-reviewed

Narufumi Kitamura, Ryusuke Nakai, Haruyasu Kohda, Keiko Furuta-Okamoto, Hiroo Iwata

BIOORGANIC & MEDICINAL CHEMISTRY　21　(22)　7175-7181　2013/11

DOI： 10.1016/j.bmc.2013.08.063 　

ISSN： 0968-0896

eISSN： 1464-3391
Heat-initiated detection for reduced glutathione with F-19 NMR probes based on modified gold nanoparticles Peer-reviewed

Narufumi Kitamura, Kazuo Tanaka, Yoshiki Chujo

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　23　(1)　281-286　2013/01

DOI： 10.1016/j.bmcl.2012.10.105 　

ISSN： 0960-894X

eISSN： 1464-3405
Reduced glutathione-resisting F-19 NMR sensors for detecting HNO Peer-reviewed

Narufumi Kitamura, Tatsuhiro Hiraoka, Kazuo Tanaka, Yoshiki Chujo

BIOORGANIC & MEDICINAL CHEMISTRY　20　(15)　4668-4674　2012/08

DOI： 10.1016/j.bmc.2012.06.013 　

ISSN： 0968-0896
Heavy metal-free F-19 NMR probes for quantitative measurements of glutathione reductase activity using silica nanoparticles as a signal quencher Peer-reviewed

Kazuo Tanaka, Narufumi Kitamura, Yoshiki Chujo

BIOORGANIC & MEDICINAL CHEMISTRY　20　(1)　96-100　2012/01

DOI： 10.1016/j.bmc.2011.11.026 　

ISSN： 0968-0896
Bimodal Quantitative Monitoring for Enzymatic Activity with Simultaneous Signal Increases in F-19 NMR and Fluorescence Using Silica Nanoparticle-Based Molecular Probes Peer-reviewed

Kazuo Tanaka, Narufumi Kitamura, Yoshiki Chujo

BIOCONJUGATE CHEMISTRY　22　(8)　1484-1490　2011/08

DOI： 10.1021/bc100381x 　

ISSN： 1043-1802
Reductive Glutathione-Responsive Molecular Release Using Water-Soluble POSS Network Polymers Peer-reviewed

Kazuo Tanaka, Wataru Ohashi, Narufumi Kitamura, Yoshiki Chujo

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN　84　(6)　612-616　2011/06

DOI： 10.1246/bcsj.20110032 　

ISSN： 0009-2673

eISSN： 1348-0634
Biodegradable Main-Chain Phosphate-Caged Fluorescein Polymers for the Evaluation of Enzymatic Activity Peer-reviewed

Kazuo Tanaka, Narufumi Kitamura, Yoshiki Chujo

MACROMOLECULES　43　(14)　6180-6184　2010/07

DOI： 10.1021/ma1009066 　

ISSN： 0024-9297
Preparation for Highly Sensitive MRI Contrast Agents Using Core/Shell Type Nanoparticles Consisting of Multiple SPIO Cores with Thin Silica Coating Peer-reviewed

Kazuo Tanaka, Asako Narita, Narufumi Kitamura, Wataru Uchiyama, Masahito Morita, Toshiro Inubushi, Yoshiki Chujo

LANGMUIR　26　(14)　11759-11762　2010/07

DOI： 10.1021/la1015077 　

ISSN： 0743-7463
Reversible signal regulation system of F-19 NMR by redox reactions using a metal complex as a switching module Peer-reviewed

Kazuo Tanaka, Narufumi Kitamura, Yuichi Takahashi, Yoshiki Chujo

BIOORGANIC & MEDICINAL CHEMISTRY　17　(11)　3818-3823　2009/06

DOI： 10.1016/j.bmc.2009.04.039 　

ISSN： 0968-0896

eISSN： 1464-3391
Improving Proton Relaxivity of Dendritic MRI Contrast Agents by Rigid Silsesquioxane Core Peer-reviewed

Kazuo Tanaka, Narufumi Kitamura, Kensuke Naka, Masahito Morita, Toshiro Inubushi, Moeko Chujo, Masaya Nagao, Yoshiki Chujo

POLYMER JOURNAL　41　(4)　287-292　2009

DOI： 10.1295/polymj.PJ2008274 　

ISSN： 0032-3896
Acceleration of guanine oxidation under visible light irradiation by photon upconversion based on triplet-triplet annihilation. Peer-reviewed

Tanaka K, Kitamura N, Inafuku K, Chujo Y

Nucleic acids symposium series (2004)　53　(53)　183-184　2009
Publisher: Oxford University Press
DOI： 10.1093/nass/nrp092 　

ISSN： 1746-8272
Assembly system of direct modified superparamagnetic iron oxide nanoparticles for target-specific MRI contrast agents Peer-reviewed

Kazuo Tanaka, Narufumi Kitamura, Masahito Morita, Toshiro Inubushi, Yoshiki Chujo

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　18　(20)　5463-5465　2008/10

DOI： 10.1016/j.bmcl.2008.09.035 　

ISSN： 0960-894X
Synthesis of Multimodal19F NMR Probes Based on Cubic Silsesquioxanes Peer-reviewed

Kazuo Tanaka, Narufumi Kitamura, Yoshiki Chujo

Photomedicine and Photobiology　30　23-24　2008/07
Publisher: The Japanese Society for Photomedicine and Photobiology
Multi-modal F-19 NMR probe using perfluorinated cubic silsesquioxane-coated silica nanoparticles for monitoring enzymatic activity Peer-reviewed

Kazuo Tanaka, Narufumi Kitamura, Kensuke Naka, Yoshiki Chujo

CHEMICAL COMMUNICATIONS　(46)　6176-6178　2008

DOI： 10.1039/b815022b 　

ISSN： 1359-7345
Properties of superparamagnetic iron oxide nanoparticles assembled on nucleic acids. Peer-reviewed

Tanaka K, Kitamura N, Chujo Y

Nucleic acids symposium series (2004)　(52)　693-694　2008

DOI： 10.1093/nass/nrn350

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Misc. 25

Imaging of Venous Thrombus Formation Mechanisms Using Synchrotron Radiation and X-ray Contrast NanoParticle

Mone KIMURA, Narufumi KITAMURA, Kohsuke GONDA

Medical Imaging Technology　44　(2)　80-85　2026/03
遷移金属錯体を基盤としたバイオイメージング

北村成史

光技術動向調査報告書　2025/03
血管新生阻害剤による腫瘍血管正常化の窓の多次元解析

當山亮太, 細沼由季, 熊谷圭悟, 宇根範和, 春田知洋, 関川明生, 米山明男, 兵藤一行, 北村成史, 権田幸祐

日本DDS学会学術集会プログラム予稿集　40回　609-609　2024/07
Publisher: 日本DDS学会
乳癌基礎研究の最前線腫瘍血管正常化の窓のナノバイオ解析と治療応用

権田幸祐, 當山亮太, 多田寛, 米山明男, 兵藤一行, 石田孝宣, 北村成史

日本乳癌学会総会プログラム抄録集　32回　26-26　2024/07
Publisher: (一社)日本乳癌学会
バイオイメージングにおける蛍光粒子材料の応用動向

北村成史

光技術動向調査報告書　2024/03
静脈血栓塞栓症の発症予測診断を目指した光計測による血栓形成機序の解明

木村森音, 佐々木洋輔, 米山明男, 兵藤一行, 春田知洋, 関川明生, 古澤直子, 檜山邦雅, 北弘志, 北村成史, 権田幸祐, 権田幸祐

日本血栓止血学会誌　35　(2)　2024

ISSN： 0915-7441
Multi-Dimensional Imaging of Venous Thrombosis Mechanisms by X-Ray Phase Contrast CT Using Synchrotron Radiation

木村森音, 佐々木洋輔, 米山明男, 兵藤一行, 春田知洋, 関川明生, 古澤直子, 檜山邦雅, 北弘志, 小林芳男, 北村成史, 権田幸祐, 権田幸祐

量子ビームサイエンスフェスタ(Web)　2023　2024
蛍光ナノ材料を用いたウイルスセンシング技術

北村成史, 権田幸祐

オプトニューズ　18　(4)　2023/03
ナノ粒子を利用した静脈血栓形成過程のマルチモーダルイメージング

木村森音, 佐々木洋輔, 北村成史, 権田幸祐

ナノ学会大会講演予稿集　21st (Web)　2023
静脈血栓塞栓症の発症予測技術への応用を目指した光イメージングによる血栓形成機序の解明

木村森音, 佐々木洋輔, 米山明男, 兵藤一行, 古澤直子, 檜山邦雅, 北弘志, 北村成史, 権田幸祐

日本血管生物医学会学術集会プログラム・抄録集　31st (CD-ROM)　2023
有機蛍光ナノ粒子を用いた生体イメージング技術

北村成史

光技術動向調査報告書　2022/03
Nanomedicine Using Photosensitive Nanoparticles

西館智尋, 金野智浩, 坂本武琉, 木下菜月, 鳥井猛流, 橋本佳樹, 川内敬子, 三好大輔, 今井陽介, 小林芳男, 山内紀子, 北村成史, 権田幸祐

ナノ学会大会講演予稿集　20th　2022
蛍光ナノ粒子を用いた乳癌バイオマーカーの高感度定量法

多田寛, 権田幸祐, 北村成史, 石田孝宣

日本臨床細胞学会雑誌　60　(Suppl.2)　382-382　2021/10
Publisher: (公社)日本臨床細胞学会
ISSN： 0387-1193

eISSN： 1882-7233
様々な手法で見る生体試料高輝度蛍光ナノ粒子を用いた病理解析による乳癌内分泌療法の耐性予測

権田幸祐, 北村成史, 石田孝宣, 多田寛

バイオイメージング　30　(2)　44-44　2021/08
Publisher: 日本バイオイメージング学会
ISSN： 1342-2634
Visualization of Biological Information and Quantitative Evaluation of Pathological Conditions Using Functional Nanoparticles

Narufumi Kitamura, Hiroshi Tada, Mayumi Takano-Kasuya, Yoshio Kobayashi, Takanori Ishida, Takasi Kamei, Kohsuke Gonda

化学工業　72　(4)　258-267　2021/04

ISSN： 0451-2014
【蛍光イメージングと癌治療】蛍光イメージングによる抗体医薬の創薬支援および薬効予測診断

権田幸祐, 根岸洋, 高野真由美, 北村成史, 古澤直子, 中野寧, 多田寛, 石田孝宣

癌と化学療法　48　(2)　170-175　2021/02
核外ERαの定量分析に基づくHR+/HER2乳癌の内分泌療法抵抗性の予測(Prediction of endocrine therapy resistance in HR+/HER2 breast cancer based on quantitative analysis of extranuclear ERα)

北村成史, 多田寛, 濱田庸, 宮下穣, 原田成美, 濱中洋平, 坪井洸樹, 大内憲明, 林慎一, 石田孝宣, 権田幸祐

日本癌学会総会記事　78回　J-1030　2019/09
Publisher: (一社)日本癌学会
ISSN： 0546-0476
血管新生阻害剤に対する薬剤耐性を回避する新たな腫瘍兵糧攻め療法の開発

加藤智尋, 北村成史, 徳永正之, 濱田庸, 鳩山恵一朗, 高野真由美, 福原武志, 今井陽介, 権田幸祐

日本心血管内分泌代謝学会学術総会プログラム及び抄録集　23rd　2019
Quantification of protein heterodimers by using fluorescent nanoparticles for breast cancer diagnosis

Narufumi Kitamura, Hiroshi Tada, Kohsuke Gonda

CANCER SCIENCE　109　521-521　2018/12

ISSN： 1349-7006
乳がん診断技術への応用を指向した蛍光ナノ粒子によるタンパク質ヘテロダイマーの定量(Quantification of protein heterodimers by using fluorescent nanoparticles for breast cancer diagnosis)

北村成史, 多田寛, 権田幸祐

日本癌学会総会記事　77回　557-557　2018/09
Publisher: 日本癌学会
ISSN： 0546-0476
Prognostic value of nuclear and non-nuclear estrogen receptor expression by immunohistochemistry with phosphor-integrated dots in hormone receptor-positive early breast cancer

Zhaorong Guo, Hiroshi Tada, Narufumi Kitamura, Yoh Hamada, Shin-ichi Hayashi, Noriaki Ohuchi, Kosuke Gonda, Takanori Ishida

CANCER RESEARCH　78　(13)　2018/07

DOI： 10.1158/1538-7445.AM2018-5243 　

ISSN： 0008-5472

eISSN： 1538-7445
金ナノ粒子を用いたがん転移リンパ節への光熱療法の開発

及川隆洋, 権田幸祐, 高野真由美, 猪瀬智也, 徳永正之, 北村成史, 小林芳男, 鳩山恵一朗, 小野寺優, 石田裕嵩, 丸山祥太, 佐藤千晃, 濱田庸, 瓶子隆弘, 櫻井直, 谷山裕亮, 亀井尚

日本外科学会定期学術集会抄録集　118回　2119-2119　2018/04
Publisher: (一社)日本外科学会
腫瘍血管に対するVEGF標的薬の効果を高感度4次元X線CTイメージングで解析する

徳永正之, 北村成史, 濱田庸, 高野真由美, 今井陽介, 権田幸祐

日本心血管内分泌代謝学会学術総会プログラム及び抄録集　21st　110　2017
新規蛍光ナノ粒子を用いた乳癌バイオマーカーの高感度定量評価

郭ショウ蓉, 多田寛, 北村成史, 濱田庸, 権田幸祐, 大内憲明

日本癌学会総会記事　75回　P-2156　2016/10
Publisher: 日本癌学会
ISSN： 0546-0476
Development and evaluation of labeled islet cells using iron oxide nanoparticles and DNA hybridization. Peer-reviewed

Nakai R, Kitamura N, Iwata Hi

Joint Annual Meeting ISMRM & ESMRMB 2014　2014

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Research Projects 14

放射線治療のアブスコパル効果を促進する腫瘍血管正常化の高分解能X線計測と応用

権田幸祐, 北村成史, 多田寛

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2025/04/01 - 2028/03/31
糖尿病におけるがんリスク増加の機序解明とがん種横断的リスク診断技術の開発

高野真由美, 北村成史, 権田幸祐

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2025/04/01 - 2028/03/31
Pharmacokinetic analysis of antibody drug conjugate in tumor cells utilizing synchrotron soft X-ray imaging

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (B)

Institution: National Institutes for Quantum Science and Technology

2023/04/01 - 2026/03/31
放射線治療に有用なアブスコパル効果の発現機序解明と応用

権田幸祐, 多田寛, 北村成史

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2022/04/01 - 2025/03/31

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放射線治療のアブスコパル効果は、研究が停滞していたが、がん免疫研究の進展で再注目されてきた。その結果、放射線照射によるがん細胞傷害が、がん抗原の露出量を増やし、これにより免疫応答を介してアブスコパル効果の発現が促進されることが分かってきた。以上の研究では、腫瘍径の値や腫瘍の一部の組織切片データを用いて解析が行われた。そのためアブスコパル効果を発現する腫瘍を、分子から組織全体に渡り統合的に計測し解析する技術に欠けており、腫瘍内で不均一に起こるアブスコパル効果の実態を正しく評価することが難しかった。本研究では、蛍光計測とX線CT計測を行い、アブスコパル効果発現の鍵となる「腫瘍微小環境」や「がん免疫応答」の経時変化に注目しつつ、両計測データを統合的に解析し、その応用としてこの効果の発現を促進・最適化させる治療法開発を試みる。以上の研究目的を実現するため、2022年度は、血管新生阻害剤を用いたアブスコパル効果の発現を誘導する放射線治療モデルの構築を試みた。この実験では、マウスの足背に1次腫瘍として癌細胞を移植し、2日後に同マウスの腰に同じ癌細胞を移植 (2次腫瘍)した。また治療条件は、コントロール群、放射線照射群 (1次腫瘍のみ)、血管新生阻害剤投与群、併用療法群の4群で行った。その結果、腫瘍径の大きさに関して治療効果データに有意差を示す実験系の確立に成功した。 2023年度は、病理解析によって治療効果の詳細な解析を行なった。その結果、2次腫瘍において、腫瘍血管のペリサイト被覆率は、血管新生阻害剤投与群と併用療法群において促進され、また腫瘍血管面積密度あたりのCD8陽性T細胞の浸潤数が、血管新生阻害剤投与群と併用療法群で優位に増えていることが分かった。この結果は、血管新生阻害剤投与によって腫瘍血管の正常化が進み、その結果、CD8陽性T細胞の浸潤数が増加したことを示唆していた。
非標準的遺伝子四重鎖への効果的薬剤送達を基盤とする新規光線力学療法の開発

北村成史, 石田孝宣, 亀井尚, 多田寛, 金野智浩, 権田幸祐

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2022/04/01 - 2025/03/31
プロテオーム・メタボローム解析による乳癌の新規悪性度評価と代謝標的治療効果予測

多田寛, 権田幸祐, 石田孝宣, 原田成美, 北村成史

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2022/04/01 - 2025/03/31
リンパネットワークのメカノバイオ解析に基づくリンパ節転移機序の解明と診断技術開発

高野真由美, 北村成史, 権田幸祐

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(C)

Institution: 東北大学

2022/04/01 - 2025/03/31
放射線治療に有用なアブスコパル効果の発現機序解明と応用

権田幸祐, 多田寛, 北村成史

Offer Organization: 日本学術振興会

System: 科学研究費助成事業

Category: 基盤研究(B)

Institution: 東北大学

2022/04/01 - 2025/03/31
Development of new cancer therapy by tumor blood vessel-specific radiation therapy

GONDA Kohsuke

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Challenging Research (Exploratory)

Institution: Tohoku University

2019/06/28 - 2022/03/31

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In this study, we aimed to develop a method for improving the antitumor effect of an angiogenesis inhibitor (bevacizumab). In 2019, conditions for low-dose radiation therapy to be used in combination therapy with bevacizumab administration were examined. In addition, the tumor-bearing mice administered with gold nanoparticles (AuNPs) were irradiated with radiation, and radiosensitization effect of AuNPs on tumor shrinkage was investigated. In 2020, to evaluate the histological effect of combination therapy of angiogenesis inhibitor and radiation therapy, we developed techniques to investigate the "morphological change of tumor blood vessels" and "functional change of tumor blood vessels". In 2021, those evaluation technologies developed in 2020 were applied to the evaluation of the antitumor effect of mice in combination with angiogenesis inhibitors and radiation therapy.
Development of early-stage cancer detection method and radiotherapy using ultra-small gold nanoparticles

Kitamura Narufumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2019/04/01 - 2022/03/31

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We prepared ultra-small gold nanoparticles with a diameter of approximately 3 nm by coating with reduced glutathione. In animal experiments using mice, it was confirmed that the nanoparticles could be excreted in urine with a half-life of less than one hour in blood. The initial plan was then slightly modified to integrate multiple gold nanoparticles on a protein basis rather than iron oxide nanoparticles. As a result, biodegradable nanoparticles with 3~4 gold nanoparticles bound on protein particles of about 10~20 nm in diameter were successfully synthesized. The results of CT imaging analysis showed that synthesized nanoparticles had different blood convection times depending on the size and properties of the scaffold protein. The gold nanoparticles can be used as a contrast agent to delineate tiny blood vessels in tumors.
Clinical significance of ABCG2/BCRP quantified by fluorescence nanoparticle in breast cancer patients undergoing neoadjuvant chemotherapy

TADA Hiroshi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research (C)

Institution: Tohoku University

2017/04/01 - 2022/03/31

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We aimed to evaluate the correlation between BCRP expression and prognosis in breast cancer tissue samples using immunohistochemistry with fluorescent phosphor-integrated dots (IHC-PIDs). A total of 37 breast cancer patients with residual cancer in the primary tumor and axillary lymph node were evaluated. BCRP levels in breast cancer tissue (PT) and metastatic lymph nodes (LN) were quantitatively detected after preoperative chemotherapy (NAC) and core needle biopsy (CNB). Biomarker assay with IHC-PIDs showed high accuracy for quantitative assessment of BCRP with low expression. High BCRP expression in Primary tumor and lymphnode after NAC was associated with worse overall survival (log-rank, p=0.0089). In conclusion, high BCRP levels are associated with poor prognosis in breast cancer patients with residual tumor in the primary tumor and lymph node after preoperative chemotherapy. These findings provide a basis for further appropriate adjuvant therapy in these patients.
Detection of HER family dimers and prediction of anticancer drug efficacy based on single particle imaging

Kitamura Narufumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (B)

Institution: Tohoku University

2017/04/01 - 2019/03/31

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We found that the HER2 / HER3 heterodimer can be detected by a technology developed using FRET between fluorescent nanoparticles developed independently. Furthermore, in order to clarify whether this physical phenomenon correctly detects the dimer, the efficiency of energy transfer is studied using a sample in which the distance between fluorescent particles is controlled, and the energy transfer is generally performed only when the dimer is formed. However, some of the particles that are the donor of the fluorescence and the particles that are the acceptor do not correspond on a one-to-one basis, or some of the particles that only exist in the vicinity without forming a dimer were detected. It is necessary to continue to consider the method of evaluation excluding
Three dimensional tissue regeneration through multipoint molecular weak association

IWATA Hiroo, OKAMOTO Yukihiro, KITAMURA Narufumi, ARIMA Yusuke

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Institution: Kyoto University

2011/04/01 - 2016/03/31

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The aim of this study is manipulation of cellular function through the modification of cell surface. We used single-stranded DNA, which was conjugated both to poly(ethylene glycol) and to a terminal phospholipid (ssDNA-PEG-lipid) for cell surface modification. The cell surface of cells can be further functionalized through DNA hybridization. We examined control of 1) biological responses and 2) cell-substrate and cell-cell attachment. 1) The modification of cell surface of endothelial cells with antioxidant-loaded liposomes reduced oxidative stress during ischemia reperfusion. The modification of cell aggregates with superparamagnetic iron oxide nanoparticles allowed for MRI monitoring post-transplantation. 2) We realized cell attachment to poly(lactic acid) scaffold in a spatially controlled manner by taking advantage of variety of DNA sequence. In addition, both cell attachment and detachment can be programmed using DNA containing the cleavage site for restriction enzyme.
Development of direct monitoring method for transplanted cells by MRI based on cell-labeling with SPIO

KITAMURA Narufumi

Offer Organization: Japan Society for the Promotion of Science

System: Grants-in-Aid for Scientific Research

Category: Grant-in-Aid for Young Scientists (B)

Institution: Kyoto University

2012/04/01 - 2014/03/31

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A labeling method for islet cells with superparamagnetic iron oxide nanoparticles (SPIOs) based on DNA hybridization is proposed for monitoring of transplanted islets by MRI, The surfaces of SPIOs were modified by via Michael addition by reacting oligo-(deoxyadenylic acid)-bearing a terminal thiol group at the 5'-end ((dA)20-SH) with maleic acid functional groups on the SPIOs. the SPIOs were immobilized on islet cells which had been pretreated with oligo-(thymidylic acid)-poly(ethylene glycol)-phospholipid conjugates ((dT)20-PEG-DPPE) through DNA hybridization. Transmission electron microscopy observations revealed that SPIOs were initially anchored on the islet cells surfaces and subsequently transferred to endosomes or exfoliated with time. The SPIO-labeled islet cells could be clearly detected as dark spots by T2 weighted MR image, whereas non-labeled islet cells could not be detected.

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Teaching Experience 7

Information and data literacy　Tohoku Universit
Topics and Discussions "Design and syhthesis of functional nanoparticles for bioimaging"　Tohoku University
Tequniques in Nuclear Medicine　Tohoku University
Fundamentals of Electrical Engineering　Tohoku University
Experiments on Radiochemistry　Tohoku University
Information Science Basics A　Tohoku University
Biochemistry　Tohoku University

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