Details of the Researcher

PHOTO

Yoshiyuki Kasahara
Section
Graduate School of Medicine
Job title
Senior Assistant Professor
Degree

  • 博士（農学）（東北大学）

  • 修士（農学）（東北大学）

e-Rad No.
20511835

Research History 9

  • 2023/04 - Present
    Tohoku University　Graduate School of Medicine

  • 2020/01 - Present
    Tohoku University　Graduate School of Medicine

  • 2018/09 - Present
    東北大学大学院医工学研究科　近未来生命情報工学分野　講師

  • 2020/06 - 2023/03
    東北大学大学院医学系研究科　母子ヘルスケア医科学共同研究講座　講師

  • 2018/08 - 2019/12
    Tohoku University　Graduate School of Medicine

  • 2017/06 - 2018/07
    Tohoku University　Graduate School of Medicine

  • 2013/10 - 2017/05
    Tohoku University　International Research Institute of Disaster Science

  • 2009/04 - 2013/09
    Tohoku University　Graduate School of Medicine

  • 2008/04 - 2009/03
    Tohoku University　Graduate School of Medicine

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Education 3

  • Tohoku University　Graduate School, Division of Agriculture

    2005/04 - 2008/03

  • Tohoku University

    2003/04 - 2005/03

  • Tohoku University　Faculty of Agriculture

    1999/04 - 2003/03

Professional Memberships 7

  • 日本分子生物学会

  • Society for Neuroscience

  • THE JAPANESE SOCIETY OF NEUROPSYCHOPHARMACOLOGY

  • THE JAPAN NEUROSCIENCE SOCIETY

  • 日本神経内分泌学会

  • THE PHYSIOLOGICAL SOCIETY OF JAPAN

  • THE JAPANESE BIOCHEMICAL SOCIETY

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Research Interests 4

  • 胎児生理学

  • 神経科学

  • 神経内分泌学

  • 精神神経生物学

Research Areas 4

  • Life sciences / Fetal medicine/Pediatrics /

  • Life sciences / Psychiatry /

  • Life sciences / Obstetrics and gynecology /

  • Life sciences / Neuroscience - general /

Papers 53

  1. Effect of Japanese Traditional Medication on Males with Diarrhea-predominant Irritable Bowel Syndrome and Gut Microbiome: A Single-arm Prospective Study Peer-reviewed

    Ryutaro Arita, Shin Takayama, Toru Tamahara, Yuichi Aoki, Soichiro Kaneko, Akiko Kikuchi, Junko Kawashima, Yoshiyuki Kasahara, Kota Ishizawa, Ritsuko Shimizu, Tadashi Ishii

    European Journal of Integrative Medicine　102512-102512　2025/06

    Publisher: Elsevier BV

    DOI： 10.1016/j.eujim.2025.102512 　

    ISSN： 1876-3820

  2. Pattern-Based Assessment of the Association of Fetal Heart Variability With Fetal Development and Maternal Heart Rate Variability. Peer-reviewed

    Namareq Widatalla, Mohanad Alkhodari, Kunihiro Koide, Chihiro Yoshida, Yoshiyuki Kasahara, Masatoshi Saito, Yoshitaka Kimura, Ahsan Khandoker

    IEEE Access　13　87941-87949　2025/05

    DOI： 10.1109/ACCESS.2025.3570326 　

  3. Multilevel Factors and Indicators of Atypical Neurodevelopment During Early Infancy in Japan: Prospective, Longitudinal, Observational Study. International-journal Peer-reviewed

    Daigo Kato, Akiko Okuno, Tetsuo Ishikawa, Shoji Itakura, Shinji Oguchi, Yoshiyuki Kasahara, Kenji Kanenishi, Yuzo Kitadai, Yoshitaka Kimura, Naoki Shimojo, Kazushige Nakahara, Akiko Hanai, Hiromichi Hamada, Haruta Mogami, Seiichi Morokuma, Kazuhiro Sakurada, Yukuo Konishi, Eiryo Kawakami

    JMIR pediatrics and parenting　8　e58337　2025/04/04

    DOI： 10.2196/58337 　

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    BACKGROUND: The early identification of developmental concerns requires understanding individual differences that may represent early signs of neurodevelopmental conditions. However, few studies have longitudinally examined how child and maternal factors interact to shape these early developmental characteristics. OBJECTIVE: We aim to identify factors from the perinatal to infant periods associated with early developmental characteristics that may precede formal diagnoses and propose a method for evaluating individual differences in neurodevelopmental trajectories. METHODS: A prospective longitudinal observational study of 147 mother-child pairs was conducted from gestation to 12 months post partum. Assessments included prenatal questionnaires and blood collection, cord blood at delivery, and postpartum questionnaires at 1, 6, and 12 months. The Modified Checklist for Autism in Toddlers (M-CHAT) was used to evaluate developmental characteristics that might indicate early signs of atypical neurodevelopment. Polychoric or polyserial correlation coefficients assessed relationships between M-CHAT scores and longitudinal variables. L2-regularized logistic regression and Shapley Additive Explanations predicted M-CHAT scores and determined feature contributions. RESULTS: Twenty-one factors (4 prenatal, 3 at birth, and 14 postnatal) showed significant associations with M-CHAT scores (adjusted P values<.05). The predictive accuracy for M-CHAT scores demonstrated reasonable predictive accuracy (area under the receiver operating characteristic curve=0.79). Key predictors included infant sleep status after 6 months (nighttime sleep duration, bedtime, and difficulties falling asleep), maternal Kessler Psychological Distress Scale scores, and Mother-to-Infant Bonding Scale scores after late gestation. CONCLUSIONS: Maternal psychological distress, mother-infant bonding, and infant sleep patterns were identified as significant predictors of early developmental characteristics that may indicate emerging developmental concerns. This study advances our understanding of early developmental assessment by providing a novel approach to identifying and evaluating early indicators of atypical neurodevelopment.

  4. Effects of pharmacological inhibition of FABP4 during gestation and lactation on offspring neurodevelopment and behavior. International-journal Peer-reviewed

    Sun Zhengkang, Hinako Kirikae, He Xiaofeng, Fumiko Yoshimachi, Minori Ikuta, Tetsuo Ohnishi, Yui Yamamoto, Hirofumi Miyazaki, Yoshiyuki Kasahara, Mai Sakai, Zhiqian Yu, Noriko Osumi, Hiroaki Tomita, Yuji Owada, Motoko Maekawa

    Neuroscience letters　853　138199-138199　2025/03/14

    DOI： 10.1016/j.neulet.2025.138199 　

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    Fatty acid-binding protein 4 (FABP4), a key regulator of lipid metabolism and inflammation, has been implicated in neurodevelopmental disorders, including autism spectrum disorder (ASD). This study investigated the effects of FABP4 inhibition during gestation and lactation on offspring neurodevelopment using the selective FABP4 inhibitor BMS309403. Female mice received BMS309403 (15 mg/kg) via oral gavage from two weeks before mating to postnatal day 28 (P28). Administration of BMS309403 to mouse dams resulted in autism-like phenotypes in male offspring (behavioral tests: n = 7-10 per group; spine analysis: 6 mice per group, n = 26-38 dendrites per group), characterized by increased dendritic spine density in the prefrontal cortex, impaired vocal communication, increased repetitive behaviors, and depression-like symptoms. Fatty acid analysis (n = 4-6 per group) revealed significant alterations in maternal and fetal lipid profiles, including elevated arachidonic acid levels in maternal plasma and increased n6PUFAs in the fetal brain, suggesting a pro-inflammatory lipid environment. Principal component analysis demonstrated distinct clustering of lipid profiles between control and BMS309403-treated groups. Cytokine analysis (n = 6 per group) indicated reductions in IL-10 and IL-12(p40) in maternal plasma and decreased TNFα in the fetal plasma, suggesting dysregulation in systemic inflammatory signaling. These findings suggest that FABP4 inhibition during the perinatal period perturbs lipid metabolism and may influence neurodevelopment through systemic metabolic changes. Although the direct effects of BMS309403 on the fetal brain cannot be excluded, alteration in maternal metabolism and placental function may have contributed to the observed neurodevelopmental changes in offspring.

  5. Impairments of social interaction in a valproic acid model in mice Peer-reviewed

    Masatoshi Ukezono, Yoshiyuki Kasahara, Chihiro Yoshida, Yuki Murakami, Takashi Okada, Yuji Takano

    Frontiers in Behavioral Neuroscience　18　2024/08/29

    Publisher: Frontiers Media SA

    DOI： 10.3389/fnbeh.2024.1430267 　

    eISSN： 1662-5153

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    Background A rodent autism spectrum disorder (ASD) model based on prenatal exposure to valproic acid (VPA) is widely recognized as a prominent model. Social behavior in rodent ASD models has primarily been evaluated through a three-chamber approach test. However, in this study, we focused on social attention in the VPA model of ASD. Methods In male C57BL/6 J mice, attentional behaviors toward conspecifics were examined through reaching tasks around 9–11 weeks of age. On embryonic day 12.5, pregnant mice underwent a subcutaneous injection of 600 mg/kg VPA sodium salt dissolved in 0.9% saline solution (VPA group) or saline solution alone (Sal group) into their neck fat. Thirty-six mice—nine each in the VPA and saline groups, and 18 partners—underwent training in reaching behavior. Subsequently, we examined whether the VPA or Sal group demonstrated focused attention toward their partners during reaching tasks. A two-way analysis of variance (ANOVA) (condition [VPA/Sal] × situation [face-to-face (attention)/not paying attention (not attention)]) was conducted on the average success rate of the situation. Additionally, we measured the duration of sniffing behavior between pairs of mice in an open field twice in total at 4 and 8 weeks of age before reaching task. The pairs were constructed by pairing a VPA or Sal group mouse with its partner, with the objective of facilitating initial encounters between the mice. A one-way ANOVA was conducted on the average duration of sniffing behavior data from 4 weeks and a second one-way ANOVA on data from 8 weeks. Results The analysis revealed a significant interaction between condition and situation in the reaching task [F (1, 28) = 6.75, p = 0.015, ηp2 = 0.19]. The simple main effect test exhibited that the “not paying attention” rate was significantly higher than that of the “face-to-face” in the VPA group (p &amp;lt; 0.01). The results revealed a not significant difference in the average duration of sniffing behavior at 4 weeks [F (3, 32) = 2.71, p = 0.06, n.s., ηp2 = 0.20], but significant difference at 8 weeks [F (3, 32) = 4.12, p &amp;lt; 0.05, ηp2 = 0.28]. Multiple comparisons using the Bonferroni method revealed significant differences in the sniffing duration at 8 weeks between from the partner toward the VPA mouse and from the partner toward the Sal mouse (p &amp;lt; 0.05). Conclusion The VPA rodent model of ASD exhibited differences in social attention compared to the saline group. By focusing on social attention and exploring various ASD models, insights can be gained from the neural mechanisms underlying gaze abnormalities during social interaction in individuals with ASD.

  6. Prediction of fetal RR intervals from maternal factors using machine learning models Peer-reviewed

    Namareq Widatalla, Mohanad Alkhodari, Kunihiro Koide, Chihiro Yoshida, Yoshiyuki Kasahara, Masatoshi Saito, Yoshitaka Kimura, Ahsan Habib Khandoker

    Scientific Reports　13　(1)　19765　2023/11/13

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-023-46920-4 　

    eISSN： 2045-2322

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    Abstract Previous literature has highlighted the importance of maternal behavior during the prenatal period for the upbringing of healthy adults. During pregnancy, fetal health assessments are mainly carried out non-invasively by monitoring fetal growth and heart rate (HR) or RR interval (RRI). Despite this, research entailing prediction of fHRs from mHRs is scarce mainly due to the difficulty in non-invasive measurements of fetal electrocardiogram (fECG). Also, so far, it is unknown how mHRs are associated with fHR over the short term. In this study, we used two machine learning models, support vector regression (SVR) and random forest (RF), for predicting average fetal RRI (fRRI). The predicted fRRI values were compared with actual fRRI values calculated from non-invasive fECG. fRRI was predicted from 13 maternal features that consisted of age, weight, and non-invasive ECG-derived parameters that included HR variability (HRV) and R wave amplitude variability. 156 records were used for training the models and the results showed that the SVR model outperformed the RF model with a root mean square error (RMSE) of 29 ms and an average error percentage (&lt; 5%). Correlation analysis between predicted and actual fRRI values showed that the Spearman coefficient for the SVR and RF models were 0.31 (P &lt; 0.001) and 0.19 (P &lt; 0.05), respectively. The SVR model was further used to predict fRRI of 14 subjects who were not included in the training. The latter prediction results showed that individual error percentages were (≤ 5%) except in 3 subjects. The results of this study show that maternal factors can be potentially used for the assessment of fetal well-being based on fetal HR or RRI.

  7. Heterozygous and homozygous gene knockout of the 5-HT1B receptor have different effects on methamphetamine-induced behavioral sensitization. International-journal Peer-reviewed

    Yuki Moriya, Yoshiyuki Kasahara, Kana Ishihara, Frank Scott Hall, Yoko Hagino, René Hen, Kazutaka Ikeda, George R Uhl, Ichiro Sora

    Behavioural pharmacology　34　(7)　393-403　2023/10/01

    DOI： 10.1097/FBP.0000000000000745 　

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    The psychostimulant drug methamphetamine (METH) causes euphoria in humans and locomotor hyperactivity in rodents by acting on the mesolimbic dopamine (DA) pathway and has severe abuse and addiction liability. Behavioral sensitization, an increased behavioral response to a drug with repeated administration, can persist for many months after the last administration. Research has shown that the serotonin 1B (5-HT1B) receptor plays a critical role in the development and maintenance of drug addiction, as well as other addictive behaviors. This study examined the role of 5-HT1B receptors in METH-induced locomotor sensitization using 5-HT1B knockout (KO) mice. To clarify the action of METH in 5-HT1B KO mice the effects of METH on extracellular levels of DA (DAec) and 5-HT (5-HTec) in the caudate putamen (CPu) and the nucleus accumbens (NAc) were examined. Locomotor sensitization and extracellular monoamine levels were determined in wild-type mice (5-HT1B +/+), heterozygous 5-HT1B receptor KO (5-HT1B +/-) mice and homozygous 5-HT1B receptor KO mice (5-HT1B -/-). Behavioral sensitization to METH was enhanced in 5-HT1B -/- mice compared to 5-HT1B +/+ mice but was attenuated in 5-HT1B +/- mice compared to 5-HT1B +/+ and 5-HT1B -/- mice. In vivo, microdialysis demonstrated that acute administration of METH increases DAec levels in the CPu and NAc of 5-HT1B KO mice compared to saline groups. In 5-HT1B +/- mice, METH increased 5-HTec levels in the CPu, and DAec levels in the NAc were higher than in others.5-HT1B receptors play an important role in regulating METH-induced behavioral sensitization.

  8. Investigating the association of maternal heart rate variability with fetal birth weight. International-journal Peer-reviewed

    Namareq Widatalla, Chihiro Yoshida, Kunihiro Koide, Yoshiyuki Kasahara, Masatoshi Saito, Yoshitaka Kimura, Ahsan Khandoker

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference　2023　1-4　2023/07

    DOI： 10.1109/EMBC40787.2023.10340182 　

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    Maternal heart rate (HR) was reported to affect birth weight and birth outcomes. Low birth weight constitutes a major health problem, and it is estimated that around 15% to 20% of births worldwide are low weight. In our previous study, we discussed the presence of similarities between maternal and fetal HRs, therefore, here, we propose to develop a parameter based on maternal and fetal HR variability (HRV) to divide data into two patterns to investigate the association of fetal birth weight with maternal HR and HRV. The parameter was derived from non-invasive records of maternal and fetal electrocardiograms (ECGs) that were collected from 78 subjects (age: 22 - 44 years old, gestational age (GA): 19 - 40 weeks). The HRV parameter was calculated by first evaluating the standard deviation (SD) of the number of R peaks occurring per 2 seconds (snRpp2s). Then, the difference between maternal and fetal snRpp2s (dmf) was calculated. The correlation between our derived parameter [dmf] with GA revealed a significant correlation that suggested the dmf's association with fetal development. The association analysis results between birthweight with maternal HR and HRV per pattern showed that significant negative correlations exist between them in one pattern. Still, the same correlations were not observed in the other pattern. This study's findings emphasise maternal health's role in fetal development assessment. In addition, this study highlights the importance of developing novel factors for properly assessing fetal development and birth outcomes.

  9. Maternal Inflammation with Elevated Kynurenine Metabolites Is Related to the Risk of Abnormal Brain Development and Behavioral Changes in Autism Spectrum Disorder Peer-reviewed

    Yuki Murakami, Yukio Imamura, Yoshiyuki Kasahara, Chihiro Yoshida, Yuta Momono, Ke Fang, Daisuke Sakai, Yukuo Konishi, Toshimasa Nishiyama

    Cells　12　(7)　1087-1087　2023/04/04

    Publisher: MDPI AG

    DOI： 10.3390/cells12071087 　

    eISSN： 2073-4409

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    Several studies show that genetic and environmental factors contribute to the onset and progression of neurodevelopmental disorders. Maternal immune activation (MIA) during gestation is considered one of the major environmental factors driving this process. The kynurenine pathway (KP) is a major route of the essential amino acid L-tryptophan (Trp) catabolism in mammalian cells. Activation of the KP following neuro-inflammation can generate various endogenous neuroactive metabolites that may impact brain functions and behaviors. Additionally, neurotoxic metabolites and excitotoxicity cause long-term changes in the trophic support, glutamatergic system, and synaptic function following KP activation. Therefore, investigating the role of KP metabolites during neurodevelopment will likely promote further understanding of additional pathophysiology of neurodevelopmental disorders, including autism spectrum disorder (ASD). In this review, we describe the changes in KP metabolism in the brain during pregnancy and represent how maternal inflammation and genetic factors influence the KP during development. We overview the patients with ASD clinical data and animal models designed to verify the role of perinatal KP elevation in long-lasting biochemical, neuropathological, and behavioral deficits later in life. Our review will help shed light on new therapeutic strategies and interventions targeting the KP for neurodevelopmental disorders.

  10. Role for μ-opioid receptor in antidepressant effects of δ-opioid receptor agonist KNT-127 Peer-reviewed

    Yuki Moriya, Yoshiyuki Kasahara, Masafumi Shimada, Yasufumi Sakakibara, Hideaki Fujii, Hiroshi Nagase, Soichiro Ide, Kazutaka Ikeda, F. Scott Hall, George R. Uhl, Ichiro Sora

    Journal of Pharmacological Sciences　151　(3)　135-141　2023/03

    DOI： 10.1016/j.jphs.2022.12.008 　

    ISSN： 1347-8613

    eISSN： 1347-8648

  11. Further notice of omics profiles of fecal and oral microbiota change in irritable bowel syndrome patients with diarrhea and symptom exacerbation Peer-reviewed

    Yukari Tanaka, Riu Yamashita, Junko Kawashima, Hiroshi Mori, Ken Kurokawa, Shinji Fukuda, Yasuhiro Gotoh, Keiji Nakamura, Tetsuya Hayashi, Yoshiyuki Kasahara, Yukuto Sato, Shin Fukudo

    Journal of Gastroenterology　58　(4)　427-428　2023/02/07

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s00535-022-01951-y 　

    ISSN： 0944-1174

    eISSN： 1435-5922

  12. Employing Support Vector Machine Regression to Estimate the Fetal Gestational Age Peer-reviewed

    Maisam Wahbah, Raghad Al Sakaji, Kiyoe Funamoto, Anita Krishnan, Yoshiyuki Kasahara, Yoshitaka Kimura, Ahsan Khandoker

    Computing in Cardiology Conference (CinC)　2022/12/31

    Publisher: Computing in Cardiology

    DOI： 10.22489/cinc.2022.170 　

    ISSN： 2325-887X

  13. Similarities between maternal and fetal RR interval tachograms and their association with fetal development. International-journal Peer-reviewed

    Namareq Widatalla, Ahsan Khandoker, Mohanad Alkhodari, Kunihiro Koide, Chihiro Yoshida, Yoshiyuki Kasahara, Yoshitaka Kimura, Masatoshi Saito

    Frontiers in physiology　13　964755-964755　2022/11

    DOI： 10.3389/fphys.2022.964755 　

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    An association between maternal and fetal heart rate (HR) has been reported but, so far, little is known about its physiological implication and importance relative to fetal development. Associations between both HRs were investigated previously by performing beat-by-beat coupling analysis and correlation analysis between average maternal and fetal HRs. However, studies reporting on the presence of similarities between maternal and fetal HRs or RR intervals (RRIs) over the short term (e.g., 5-min) at different gestational ages (GAs) are scarce. Here, we demonstrate the presence of similarities in the variations exhibited by maternal and fetal RRl tachograms (RRITs). To quantify the same similarities, a cross-correlation (CC) analysis between resampled maternal and fetal RRITs was conducted; RRITs were obtained from non-invasive electrocardiogram (ECG). The degree of similarity between maternal and fetal RRITs (bmfRRITs) was quantified by calculating four CC coefficients. CC analysis was performed for a total of 330 segments (two 5-min segments from 158 subjects and one 5-min from 14 subjects). To investigate the association of the similarity bmfRRITs with fetal development, the linear correlation between the calculated CC coefficients and GA was calculated. The results from the latter analysis showed that similarities bmfRRITs are common occurrences, they can be negative or positive, and they increase with GA suggesting the presence of a regulation that is associated with proper fetal development. To get an insight into the physiological mechanisms involved in the similarity bmfRRITs, the association of the same similarity with maternal and fetal HR variability (HRV) was investigated by comparing the means of two groups in which one of them had higher CC values compared to the other. The two groups were created by using the data from the 158 subjects where fetal RRI (fRRI) calculation from two 5-min ECG segments was feasible. The results of the comparison showed that the maternal very low frequency (VLF) HRV parameter is potentially associated with the similarity bmfRRITs implying that maternal hormones could be linked to the regulations involved in the similarity bmfRRITs. Our findings in this study reinforce the role of the maternal intrauterine environment on fetal development.

  14. Correlation between maternal and fetal heart rate increases with fetal mouse age in typical development and is disturbed in autism mouse model treated with valproic acid. International-journal Peer-reviewed

    Namareq Widatalla, Ahsan Khandoker, Chihiro Yoshida, Kana Nakanishi, Miyabi Fukase, Arisa Suzuki, Masatoshi Saito, Yoshitaka Kimura, Yoshiyuki Kasahara

    Frontiers in psychiatry　13　998695-998695　2022/11

    DOI： 10.3389/fpsyt.2022.998695 　

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    INTRODUCTION: Autism spectrum disorder (ASD) is considered a significant behavioral problem that is characterized by impairment in social interaction and communication. It is believed that some cases of ASD originate in the intrauterine maternal environment. Therefore, we hypothesized that there might be qualitative changes in the interaction between the mother and fetus in ASD during the prenatal period, hence, we investigated the similarity patterns between maternal and fetal heart rate (HR). METHODS: In this study, we first demonstrate the presence and formation of similarities between maternal and fetal RR interval (RRI) collected from typical developmental mice at different embryonic days (EDs), ED13.5, ED15.5, ED17.5, and ED18.5. The similarities were quantified by means of cross-correlation (CC) and magnitude-squared coherence (MSC) analyses. Correlation analysis between the CC coefficients and EDs and between MSC coefficients and EDs showed that the same coefficients increase with EDs, suggesting that similarities between maternal and fetal RRI are associated with typical fetal development. Next, because maternal and fetal similarities were indicative of development, a comparison analysis between the autism mouse model (injected with valproic acid (VPA)), and the control group (injected with saline) was performed for ED15.5 and ED18.5. RESULTS: The results of the comparison showed that the CC and MSC coefficients of VPA fetuses were significantly lower than that of the control group. The lower coefficients in VPA-treated mice suggest that they could be one of the features of ASD symptoms. The findings of this study can assist in identifying potential ASD causes during the prenatal period.

  15. Omics profiles of fecal and oral microbiota change in irritable bowel syndrome patients with diarrhea and symptom exacerbation. Peer-reviewed

    Yukari Tanaka, Riu Yamashita, Junko Kawashima, Hiroshi Mori, Ken Kurokawa, Shinji Fukuda, Yasuhiro Gotoh, Keiji Nakamura, Tetsuya Hayashi, Yoshiyuki Kasahara, Yukuto Sato, Shin Fukudo

    Journal of gastroenterology　57　(10)　748-760　2022/07/30

    DOI： 10.1007/s00535-022-01888-2 　

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    BACKGROUND: Irritable bowel syndrome (IBS) is a disorder of gut-brain interaction, including dysregulation of the hypothalamic-pituitary-adrenal axis with salivary cortisol changes. However, the role of gastrointestinal microbiota during IBS symptom exacerbation remains unclear. We tested the hypothesis that the microbial species, gene transcripts, and chemical composition of fecal and oral samples are altered during the exacerbation of IBS symptoms. METHODS: Fecal, salivary, and dental plaque samples were collected at baseline from 43 men with IBS with diarrhea (IBS-D) and 40 healthy control (HC) men. Samples in the IBS-D patients were also collected during symptom exacerbation. The composition of the fecal microbiota was determined by analyzing the 16S rRNA gene, RNA-based metatranscriptome, and metabolites in samples from HC and IBS patients with and without symptom exacerbation. Oral samples were also analyzed using omics approaches. RESULTS: The fecal microbiota during IBS symptom exacerbation exhibited significant differences in the phylogenic pattern and short-chain fatty acid compared with fecal samples during defecation when symptoms were not exacerbated. Although there were no significant differences in the phylogenic pattern of fecal microbiota abundance between HCs and IBS-D patients, significant differences were detected in the expression patterns of bacterial transcriptomes related to butyrate production and neuroendocrine hormones, including tryptophan-serotonin-melatonin synthesis and glutamine/GABA. The composition of plaque microbiota was different between HC and IBS-D patients during normal defecation. CONCLUSIONS: Our findings suggest that colonic host-microbial interactions are altered in IBS-D patients during exacerbation of symptoms. There were no overlaps between feces and oral microbiomes.

  16. Model-based estimation of QT intervals of mouse fetal electrocardiogram. International-journal Peer-reviewed

    Namareq Widatalla, Kiyoe Funamoto, Motoyoshi Kawataki, Chihiro Yoshida, Kenichi Funamoto, Masatoshi Saito, Yoshiyuki Kasahara, Ahsan Khandoker, Yoshitaka Kimura

    Biomedical engineering online　21　(1)　45-45　2022/06/29

    DOI： 10.1186/s12938-022-01015-5 　

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    BACKGROUND: Abnormal prolongation in the QT interval or long QT syndrome (LQTS) is associated with several cardiac complications such as sudden infant death syndrome (SIDS). LQTS is believed to be linked to genetic mutations which can be understood by using animal models, such as mice models. Nevertheless, the research related to fetal QT interval in mice is still limited because of challenges associated with T wave measurements in fetal electrocardiogram (fECG). Reliable measurement of T waves is essential for estimating their end timings for QT interval assessment. RESULTS: A mathematical model was used to estimate QT intervals. Estimated QT intervals were validated with Q-aortic closure (Q-Ac) intervals of Doppler ultrasound (DUS) and comparison between both showed good agreement with a correlation coefficient higher than 0.88 (r > 0.88, P < 0.05). CONCLUSION: Model-based estimation of QT intervals can help in better understanding of QT intervals in fetal mice.

  17. Classification of pacemaker dynamics in the mouse intestine by field potential microimaging Peer-reviewed

    Naoko Iwata, Chiho Takai, Naoto Mochizuki, Mariko Yamauchi, Noriyuki Kaji, Yoshiyuki Kasahara, Shinsuke Nakayama

    Biosensors and Bioelectronics: X　10　2022/05

    DOI： 10.1016/j.biosx.2022.100111 　

    eISSN： 2590-1370

  18. Investigating the effect of cholinergic and adrenergic blocking agents on maternal-fetal heart rates and their interactions in mice fetuses Peer-reviewed

    Ahsan H. Khandoker, Maisam Wahbah, Chihiro Yoshida, Yoshiyuki Kasahara, Kiyoe Funamoto, Kyuichi Niizeki, Yoshitaka Kimura

    Biology open　11　(4)　2022/04

    DOI： 10.1242/bio.058999 　

    eISSN： 2046-6390

  19. Effect of Valproic Acid on Maternal - Fetal Heart Rates and Coupling in Mice on Embryonic day 15.5 (E15.5) Peer-reviewed

    Namareq Widatalla, Ahsan Khandoker, Chihiro Yoshida, Kana Nakanishi, Miyabi Fukase, Arisa Suzuki, Yoshiyuki Kasahara, Masatoshi Saito, Yoshitaka Kimura

    Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS　5504-5507　2021

    DOI： 10.1109/EMBC46164.2021.9630153 　

    ISSN： 1557-170X

  20. The Effects of Maternal Interleukin-17A on Social Behavior, Cognitive Function, and Depression-Like Behavior in Mice with Altered Kynurenine Metabolites Peer-reviewed

    Yuki Murakami, Yukio Imamura, Yoshiyuki Kasahara, Chihiro Yoshida, Yuta Momono, Ke Fang, Toshimasa Nishiyama, Daisuke Sakai, Yukuo Konishi

    International Journal of Tryptophan Research　14　2021

    DOI： 10.1177/11786469211026639 　

    eISSN： 1178-6469

  21. Assessments of Heart Rate and Sympathetic and Parasympathetic Nervous Activities of Normal Mouse Fetuses at Different Stages of Fetal Development Using Fetal Electrocardiography. International-journal Peer-reviewed

    Yoshiyuki Kasahara, Chihiro Yoshida, Masatoshi Saito, Yoshitaka Kimura

    Frontiers in physiology　12　652828-652828　2021

    DOI： 10.3389/fphys.2021.652828 　

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    Heart rate is controlled by the activity of the autonomic nervous system: the sympathetic and parasympathetic nervous systems increase and suppress heart rate, respectively. To evaluate the activity of the autonomic nervous system, it is possible to determine heart rate variability using electrocardiography (ECG). During the fetal period, the heart and autonomic nerves develop in coordination; however, physiological changes, including autonomic nervous activities that occur during the fetal stage, remain largely unknown. Therefore, in this study, we measured ECG signals of mouse fetuses using our established method to evaluate the development of heart rate and autonomic nervous activity at different fetal developmental stages. We found that heart rate was significantly increased in fetal mice at embryonic day (E) 18.5 compared with that at E13.5, E15.5, and E17.5, indicating that fetal heart rate increases only at the stage immediately prior to birth. Interestingly, fetal parasympathetic nervous activity was reduced at E17.5 and E18.5 compared with that at E13.5, whereas fetal sympathetic nervous activity remained unchanged, at least from E13.5 to E18.5. These results indicate that parasympathetic activity rather than sympathetic activity affects fetal heart rate and that the decrease in parasympathetic activity toward the end of pregnancy could result in the observed increase in fetal heart rate.

  22. Alterations in the autonomic nerve activities of prenatal autism model mice treated with valproic acid at different developmental stages. International-journal Peer-reviewed

    Yoshiyuki Kasahara, Chihiro Yoshida, Kana Nakanishi, Miyabi Fukase, Arisa Suzuki, Yoshitaka Kimura

    Scientific reports　10　(1)　17722　2020/10/20

    DOI： 10.1038/s41598-020-74662-0 　

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    Autism spectrum disorder (ASD) is characterized by impairment of social communication, repetitive behavior and restrictive interest. The risk of ASD is strongly associated with the prenatal period; for instance, the administration of valproic acid (VPA) to pregnant mothers increases risk of ASD in the child. Patients with ASD often exhibit an alteration in the autonomic nervous system. In this study, we assessed the autonomic nervous activity at each prenatal developmental stage of model mice of ASD treated with VPA, to clarify the relationship between timing of exposure and ASD symptoms. The assessment of the autonomic nervous activity was performed based on the analysis of electrocardiography data collected from fetal and adult mice. Interestingly, VPA model mouse fetuses exhibited a significantly lower activity of the sympathetic nervous system. In contrast, sympathetic nervous activity at P0 was significantly higher. In adult VPA model mice, the parasympathetic activity of female VPA mice was suppressed. Moreover, female VPA mice showed reduced the parasympathetic activity after exposure to restraint stress. These results suggest that the autonomic nervous activity of VPA model mice was altered from the fetal stage, and that the assessment of autonomic nervous activities at an early developmental stage could be useful for the understanding of ASD.

  23. Effect of Anesthesia on Fetal-Maternal Heart Rate Variability and Coupling in Pregnant Mice and Fetuses Peer-reviewed

    Ahsan H. Khandoker, Maisam Wahbah, Chihiro Yoshida, Yoshitaka Kimura, Yoshiyuki Kasahara

    Computing in Cardiology　2020-September　2020/09/13

    DOI： 10.22489/CinC.2020.197 　

    ISSN： 2325-8861

    eISSN： 2325-887X

  24. Oxytocin Ameliorates Impaired Behaviors of High Fat Diet-Induced Obese Mice International-journal Peer-reviewed

    Ryotaro Hayashi, Yoshiyuki Kasahara, Shizu Hidema, Satoshi Fukumitsu, Kiyotaka Nakagawa, Katsuhiko Nishimori

    Frontiers in Endocrinology　11　379-379　2020/07/03

    Publisher: Frontiers Media SA

    DOI： 10.3389/fendo.2020.00379 　

    eISSN： 1664-2392

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    Excessive intake of fat is a major risk factor for lifestyle-related diseases such as heart disease and also affects brain function such as object recognition memory, social recognition, anxiety behavior, and depression-like behavior. Although oxytocin (OXT) has been reported to improve object recognition, social recognition, anxiety behavior, and depression-like behavior in specific conditions, previous studies did not explore the impact of OXT in high-fat diet (HFD)-fed mice. Furthermore, it remains unclear whether intake of HFD affects OXT/oxytocin receptor (OXTR) in the brain. Here, we demonstrated that peripheral OXT administration improves not only social recognition but also object recognition and depressive-like behavior in HFD-fed mice. In contrast, peripheral OXT administration to HFD-fed male mice increased fear and anxiety-related behavior. In addition, we observed that intake of HFD decreased OXTR and c-fos mRNA expression in the hippocampus, specifically. Furthermore, peripheral OXT administration increased OXT mRNA expression in the hypothalamus. Altogether, these findings suggest that OXT has the potential to improve various recognition memory processes via peripheral administration but also has side effects that increase fear-related behavior in males.

  25. Model based estimation of QT intervals in non-invasive fetal ECG signals. International-journal Peer-reviewed

    Namareq Widatalla, Yoshiyuki Kasahara, Yoshitaka Kimura, Ahsan Khandoker

    PLOS ONE　15　(5)　e0232769　2020/05

    DOI： 10.1371/journal.pone.0232769 　

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    The end timing of T waves in fetal electrocardiogram (fECG) is important for the evaluation of ST and QT intervals which are vital markers to assess cardiac repolarization patterns. Monitoring malignant fetal arrhythmias in utero is fundamental to care in congenital heart anomalies preventing perinatal death. Currently, reliable detection of end of T waves is possible only by using fetal scalp ECG (fsECG) and fetal magnetocardiography (fMCG). fMCG is expensive and less accessible and fsECG is an invasive technique available only during intrapartum period. Another safer and affordable alternative is the non-invasive fECG (nfECG) which can provide similar assessment provided by fsECG and fMECG but with less accuracy (not beat by beat). Detection of T waves using nfECG is challenging because of their low amplitudes and high noise. In this study, a novel model-based method that estimates the end of T waves in nfECG signals is proposed. The repolarization phase has been modeled as the discharging phase of a capacitor. To test the model, fECG signals were collected from 58 pregnant women (age: (34 ± 6) years old) bearing normal and abnormal fetuses with gestational age (GA) 20-41 weeks. QT and QTc intervals have been calculated to test the level of agreement between the model-based and reference values (fsECG and Doppler Ultrasound (DUS) signals) in normal subjects. The results of the test showed high agreement between model-based and reference values (difference < 5%), which implies that the proposed model could be an alternative method to detect the end of T waves in nfECG signals.

  26. Evaluating the reliability of fetal electrocardiogram monitoring that acts via the abdominal wall of pregnant rabbits Peer-reviewed

    ITO, Takuya, SUGIBAYASHI, Rika, SATO, Naoaki, OWADA, Kazunari, SUDO, Kazuhiko, ODAGIRI, Naoko, KASAHARA, Yoshiyuki, YAEGASHI, Nobuo, KIMURA, Yoshitaka

    Translational and Regulatory Sciences　2　(1)　14-18　2020/03

  27. Effect of Propranolol and Its Dosages on Maternal-Fetal Heart Rates Coupling in Pregnant Mice and Fetuses Peer-reviewed

    Ahsan H. Khandoker, Chihiro Yoshida, Yoshiyuki Kasahara, Kiyoe Funamoto, Yoshitaka Kimura

    Computing in Cardiology　2019-September　2019/09

    DOI： 10.23919/CinC49843.2019.9005883 　

    ISSN： 2325-8861

    eISSN： 2325-887X

  28. A Recurrence Plot Based Method for the Detection of End of T-Wave in Abnormal Non-Invasive Fetal Electrocardiogram Signals Peer-reviewed

    Namareq Widatalla, Ahsan Khandoker, Yoshiyuki Kasahara, Yoshitaka Kimura

    Computing in Cardiology　2019-September　2019/09

    DOI： 10.23919/CinC49843.2019.9005572 　

    ISSN： 2325-8861

    eISSN： 2325-887X

  29. Effect of β-blocker on maternal-fetal heart rates and coupling in pregnant mice and fetuses. International-journal Peer-reviewed

    Ahsan H Khandoker, Chihiro Yoshida, Yoshiyuki Kasahara, Kiyoe Funamoto, Kana Nakanishi, Miyabi Fukase, Keiichi Kanda, Kyuichi Niizeki, Yoshitaka Kimura

    Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference　2019　1784-1787　2019/07

    DOI： 10.1109/EMBC.2019.8856719 　

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    The aim of this preliminary study is to look how maternal-fetal heart rates and their coupling patterns are influenced by injection of β blocker(propranolol) into pregnant mice. Total of 6 pregnant female mice were divided into two groups [control (N=3) and β blockade (N=3)]. On 17.5-day mean heart rate of mothers and fetuses (MHR and FHR) were simultaneously measured for 20 minutes (10 minutes under normal condition and 10 minutes with saline (to control group) and propranolol (to the β blockade group) solution by using an invasive maternal and fetal electrocardiogram techniques with needle electrodes. Results show that FHR decreased and maternal-fetal heart rate coupling (λ) patterns changed with propranolol infusion (no change with saline). Statistical test showed that changes (increase/decrease from pre to post values) in mean, rmssd and power spectral density (PSD) (2~4 Hz)) of MHR, short term variability of FHR, PSD (0.0~1.0 Hz) of FHR and λ were found to be significantly associated with treatment types (saline to propranolol). The presented results and protocol allow for assessment of β adrenergic control of maternal and fetal heart, which will further enhance the value of the mouse as a model of heritable human pregnancy and hypertension.

  30. Detection of End of T-wave in Fetal ECG Using Recurrence Plots. International-journal Peer-reviewed

    Namareq Widatalla, Ahsan Khandoker, Yoshiyuki Kasahara, Yoshitaka Kimura

    Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference　2019　2618-2621　2019/07

    DOI： 10.1109/EMBC.2019.8856737 　

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    Automatic detection of fetal ECG features can assist in diagnosis of fetal cardiac complications and may reduce the time required for diagnosis. Detection of the end of the repolarization period wave in ECG has been proven challenging due to its low amplitude and low frequency range. The prolongation of end of T-wave is associated with sudden cardiac death, thus, methods that can accurately pinpoint it is highly desirable for early diagnosis of cardiac diseases. In this paper, a technique based on recurrence plots is developed for the detection of end of T-wave. The developed technique was tested on maternal ECG (mECG), fetal scalp ECG (fsECG) and non-invasive fetal ECG (nfECG) records. The technique was able to detect end of T-waves in all of the mECG beats, 75% of the non-invasive fECG beats (verified by simultaneously captured doppler ultrasound signals) and 78% of the fsECG beats. Detection of fECG signals were more challenging than mECG signals due to the noise and their low amplitude T-waves.

  31. LGR4 is essential for R-spondin1-mediated suppression of food intake via pro-opiomelanocortin. International-journal Peer-reviewed

    Ayano Otsuka, Ayana Jinguji, Yuko Maejima, Yoshiyuki Kasahara, Kenju Shimomura, Shizu Hidema, Katsuhiko Nishimori

    Bioscience, biotechnology, and biochemistry　83　(7)　1336-1342　2019/07

    DOI： 10.1080/09168451.2019.1591266 　

    ISSN： 0916-8451

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    Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4) suppresses food intake after its activation by binding of its ligands, R-spondins. We investigated the mechanism of food intake suppression by R-spondin1 in a region-specific Lgr4 gene knockout (LGR4 cKO) mouse model, generated by deletion of the Lgr4 gene in arcuate nucleus (ARC) using Lgr4fx/fx mice combined with infection of an AAV-Cre vector. After R-spondin1 administration, LGR4 cKO mice didn't exhibit a suppressed appetite, compared to that in control mice, which received a vehicle. In ARC of LGR4 cKO mice, Pomc mRNA expression was reduced, leading to suppressed food intake. On the other hand, neurons-specific LGR4 KO mice exhibited no differences in Pomc expression, and no structural differences were observed in the ARC of mutant mice. These results suggest that LGR4 is an essential part of the mechanism, inducing Pomc gene expression with R-spondin1 in ARC neurons in mice, thereby regulating feeding behavior. Abbreviations: LGR4: Leucine-rich repeat-containing G-protein coupled receptor 4; RSPOs: roof plate-specific spondins; ARC: arcuate nucleus; AAV: adeno associated virus; POMC: pro-opiomelanocortin; CART: cocaine and amphetamine-regulated transcript; NPY: neuropeptide Y; AgRP: agouti-related peptide; Axin2: axis inhibition protein 2; Lef1: lymphoid enhancer binding factor 1; ccnd1: cyclin D1.

  32. Repeated methamphetamine treatment increases spine density in the nucleus accumbens of serotonin transporter knockout mice. International-journal Peer-reviewed

    Kasahara Y, Sakakibara Y, Hiratsuka T, Moriya Y, Lesch KP, Hall FS, Uhl GR, Sora I

    Neuropsychopharmacology reports　39　(2)　130-133　2019/02

    DOI： 10.1002/npr2.12049 　

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    AIM: Repeated psychostimulant drug treatment, including methamphetamine, in rodents readily produces behavioral sensitization, which reflects altered brain function caused by repeated drug exposure. Dendritic remodeling of medium spiny neurons in the nucleus accumbens is thought to be an essential mechanism underlying behavioral sensitization. We recently showed that chronic methamphetamine treatment did not produce behavioral sensitization in serotonin transporter knockout mice. METHODS: In this study, we report the spine density of medium spiny neurons in the nucleus accumbens after repeated methamphetamine injection to examine morphological alterations in serotonin transporter knockout mice. RESULTS: Golgi-COX staining clearly showed that the spine density of medium spiny neurons in the nucleus accumbens increased following repeated methamphetamine treatment in both wild-type and serotonin transporter knockout mice. CONCLUSIONS: Our results suggested that augmented serotonergic neurotransmission produced by serotonin transporter deletion prevents the development of behavioral sensitization in a manner that is independent of dendritic remodeling in the nucleus accumbens.

  33. Regulation of maternal-fetal heart rates and coupling in mice fetuses. International-journal Peer-reviewed

    Khandoker AH, Yoshida C, Kasahara Y, Funamoto K, Nakanishi K, Fukase M, Kanda K, Haroun I, Niizeki K, Kimura Y

    Conf Proc IEEE Eng Med Biol Soc.　2018　5257-5260　2018/07

    DOI： 10.1109/EMBC.2018.8513463 　

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    The aim of this preliminary study is to investigate if there is any evidence of maternal-fetal heart rate coupling in mice fetuses and how the coupling patterns are regulated by vagal nervous system on beat by beat. Total of 6 pregnant female mice were divided into two groups [control (N=3) and vagal blockade (N=3)]. On 17.5-day beat-to-beat heart rates of mothers and fetuses (MHR and FHR) were simultaneously measured for 20 minutes (10 minutes under normal condition and 10 minutes with saline (to control group) and atropine (to the vagal blockade group)) solution by using an invasive maternal and fetal electrocardiogram techniques with needle electrodes. Results show that occasional strong maternal-fetal heart rate coupling (strength was measured by $\lambda$) appeared and its patterns changed with atropine infusion (no change with saline). Additionally, fisher's exact test shows that changes (increase/decrease from pre to post injection values) in mean, rmssd and power spectral density (PSD) (2~4 Hz) of MHR, rmssd FHR and PSD (2~4 Hz) of${\lambda }$were found to be significantly (p<0.05) associated with treatment types (saline/ atropine). The presented results and protocol allow for the first time in the assessment of autonomic regulation of maternal and fetal heart and their interactions, which will further enhance the value of the mouse as a murine model of heritable human pregnancy and perinatal complications due to maternal conditions.

  34. Relationship between short term variability (STV) and onset of cerebral hemorrhage at ischemia-reperfusion load in fetal growth restricted (FGR) mice Peer-reviewed

    Takahiro Minato, Takuya Ito, Yoshiyuki Kasahara, Sayaka Ooshio, Tomofumi Fushima, Akiyo Sekimoto, Nobuyuki Takahashi, Nobuo Yaegashi, Yoshitaka Kimura

    Frontiers in Physiology　9　(MAY)　478　2018/05/18

    Publisher: Frontiers Media S.A.

    DOI： 10.3389/fphys.2018.00478 　

    ISSN： 1664-042X

  35. Microglial production of TNF-alpha is a key element of sustained fear memory Peer-reviewed

    Zhiqian Yu, Tiotaka Fukushima, Chiaki Ono, Mai Sakai, Yoshiyuki Kasahara, Yoshie Kikuchi, Nicole Gunawansa, Yuta Takahashi, Hiroo Matsuoka, Satoshi Kida, Hiroaki Tomita

    BRAIN BEHAVIOR AND IMMUNITY　59　313-321　2017/01

    DOI： 10.1016/j.bbi.2016.08.011 　

    ISSN： 0889-1591

    eISSN： 1090-2139

  36. Role of the Oxytocin Receptor Expressed in the Rostral Medullary Raphe in Thermoregulation During Cold Conditions Peer-reviewed

    Yoshiyuki Kasahara, Yuko Tateishi, Yuichi Hiraoka, Ayano Otsuka, Hiroaki Mizukami, Keiya Ozawa, Keisuke Sato, Shizu Hidema, Katsuhiko Nishimori

    FRONTIERS IN ENDOCRINOLOGY　6　180　2015/11

    DOI： 10.3389/fendo.2015.00180 　

    ISSN： 1664-2392

  37. The Regulation of Oxytocin Receptor Gene Expression during Adipogenesis Peer-reviewed

    K. J. Yi, K. H. So, Y. Hata, Y. Suzuki, D. Kato, K. Watanabe, H. Aso, Y. Kasahara, K. Nishimori, C. Chen, K. Katoh, S. G. Roh

    JOURNAL OF NEUROENDOCRINOLOGY　27　(5)　335-342　2015/05

    DOI： 10.1111/jne.12268 　

    ISSN： 0953-8194

    eISSN： 1365-2826

  38. Sex differences in the effects of adolescent social deprivation on alcohol consumption in mu-opioid receptor knockout mice Peer-reviewed

    Yuki Moriya, Yoshiyuki Kasahara, F. Scott Hall, Yasufumi Sakakibara, George R. Uhl, Hiroaki Tomita, Ichiro Sora

    PSYCHOPHARMACOLOGY　232　(8)　1471-1482　2015/04

    DOI： 10.1007/s00213-014-3784-y 　

    ISSN： 0033-3158

    eISSN： 1432-2072

  39. Attenuated methamphetamine-induced locomotor sensitization in serotonin transporter knockout mice is restored by serotonin 1B receptor antagonist treatment Peer-reviewed

    Moe Igari, Hao-Wei Shen, Yoko Hagino, Setsu Fukushima, Yoshiyuki Kasahara, Klaus-Peter Lesch, Dennis L. Murphy, Frank Scott Hall, George R. Uhl, Kazutaka Ikeda, Nobuo Yaegashi, Ichiro Sora

    BEHAVIOURAL PHARMACOLOGY　26　(1-2)　167-179　2015/02

    DOI： 10.1097/FBP.0000000000000120 　

    ISSN： 0955-8810

    eISSN： 1473-5849

  40. Specific regions display altered grey matter volume in mu-opioid receptor knockout mice: MRI voxel-based morphometry Peer-reviewed

    Kazumasu Sasaki, Akira Sumiyoshi, Hiroi Nonaka, Yoshiyuki Kasahara, Kazutaka Ikeda, F. Scott Hall, George R. Uhl, Masahiko Watanabe, Ryuta Kawashima, Ichiro Sora

    BRITISH JOURNAL OF PHARMACOLOGY　172　(2)　654-667　2015/01

    DOI： 10.1111/bph.12807 　

    ISSN： 0007-1188

    eISSN： 1476-5381

  41. Region-Specific Dendritic Spine Loss of Pyramidal Neurons in Dopamine Transporter Knockout Mice Peer-reviewed

    Y. Kasahara, Y. Arime, F. S. Hall, G. R. Uhl, I. Sora

    CURRENT MOLECULAR MEDICINE　15　(3)　237-244　2015

    ISSN： 1566-5240

    eISSN： 1875-5666

  42. Specific Regions Display Altered Grey Matter Volume in m-Opioid Receptor Knockout Mice: MRI Voxel-based Morphometry Peer-reviewed

    Ichiro Sora, Kazumasu Sasaki, Akira Sumiyoshi, Hiroi Nonaka, Yoshiyuki Kasahara, Kazutaka Ikeda, F. Scott Hall, George R. Uhl, Masahiko Watanabe, Ryuta Kawashima

    NEUROPSYCHOPHARMACOLOGY　39　(2)　S134-S135　2014/12

    DOI： 10.1111/bph.12807 　

    ISSN： 0893-133X

    eISSN： 1740-634X

  43. Fluorescently Activated Cell Sorting Followed by Microarray Profiling of Helper T Cell Subtypes from Human Peripheral Blood Peer-reviewed

    Chiaki Ono, Zhiqian Yu, Yoshiyuki Kasahara, Yoshie Kikuchi, Naoto Ishii, Hiroaki Tomita

    PLOS ONE　9　(11)　e111405-e111405　2014/11

    DOI： 10.1371/journal.pone.0111405 　

    ISSN： 1932-6203

  44. Developmental alterations in anxiety and cognitive behavior in serotonin transporter mutant mice Peer-reviewed

    Yasufumi Sakakibara, Yoshiyuki Kasahara, F. Scott Hall, Klaus-Peter Lesch, Dennis L. Murphy, George R. Uhl, Ichiro Sora

    PSYCHOPHARMACOLOGY　231　(21)　4119-4133　2014/10

    DOI： 10.1007/s00213-014-3554-x 　

    ISSN： 0033-3158

    eISSN： 1432-2072

  45. Oxytocin Receptor in the Hypothalamus Is Sufficient to Rescue Normal Thermoregulatory Function in Male Oxytocin Receptor Knockout Mice Peer-reviewed

    Yoshiyuki Kasahara, Keisuke Sato, Yuki Takayanagi, Hiroaki Mizukami, Keiya Ozawa, Shizu Hidema, Kyoung-Ha So, Teruo Kawada, Nao Inoue, Ikuo Ikeda, Sang-Gun Roh, Keiichi Itoi, Katsuhiko Nishimori

    ENDOCRINOLOGY　154　(11)　4305-4315　2013/11

    DOI： 10.1210/en.2012-2206 　

    ISSN： 0013-7227

    eISSN： 1945-7170

  46. Impaired cliff avoidance reaction in dopamine transporter knockout mice Peer-reviewed

    Motoyasu Yamashita, Yasufumi Sakakibara, F. Scott Hall, Yohtaro Numachi, Sumiko Yoshida, Hideaki Kobayashi, Osamu Uchiumi, George R. Uhl, Yoshiyuki Kasahara, Ichiro Sora

    Psychopharmacology　227　(4)　741-749　2013/06

    DOI： 10.1007/s00213-013-3009-9 　

    ISSN： 0033-3158 1432-2072

  47. Exclusive expression of VMAT2 in noradrenergic neurons increases viability of homozygous VMAT2 knockout mice Peer-reviewed

    Arihisa Ohara, Yoshiyuki Kasahara, Hideko Yamamoto, Harumi Hata, Hideaki Kobayashi, Yohtaro Numachi, Ichiro Miyoshi, F. Scott Hall, George R. Uhl, Kazutaka Ikeda, Ichiro Sora

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　432　(3)　526-532　2013/03

    DOI： 10.1016/j.bbrc.2013.02.014 　

    ISSN： 0006-291X

  48. Serotonergic involvement in the amelioration of behavioral abnormalities in dopamine transporter knockout mice by nicotine Peer-reviewed

    Osamu Uchiumi, Yoshiyuki Kasahara, Asami Fukui, F. Scott Hall, George R. Uhl, Ichiro Sora

    NEUROPHARMACOLOGY　64　348-356　2013/01

    DOI： 10.1016/j.neuropharm.2012.07.016 　

    ISSN： 0028-3908

  49. Cortico-Subcortical Neuromodulation Involved in the Amelioration of Prepulse Inhibition Deficits in Dopamine Transporter Knockout Mice Peer-reviewed

    Yosefu Arime, Yoshiyuki Kasahara, F. Scott Hall, George R. Uhl, Ichiro Sora

    NEUROPSYCHOPHARMACOLOGY　37　(11)　2522-2530　2012/10

    DOI： 10.1038/npp.2012.114 　

    ISSN： 0893-133X

  50. Neuronal development of the hyperdopaminergic animal model Peer-reviewed

    Yoshiyuki Kasahara, Yosefu Arime, Yumiko Kubo, Asami Fukui, Ichiro Sora

    Japanese Journal of Neuropsychopharmacology　31　(5-6)　195-199　2011/11

    Publisher: 5-6

    ISSN： 1340-2544

  51. Generation of Adeno-Associated Virus Vector Enabling Functional Expression of Oxytocin Receptor and Fluorescence Marker Genes Using the Human eIF4G Internal Ribosome Entry Site Element Peer-reviewed

    Keisuke Sato, Shiori Date, Yumi Aoyagi, Yoshiyuki Kasahara, Akihiko Nawa, Hiroaki Mizukami, Shizu Hidema, Keiya Ozawa, Katsuhiko Nishimori

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　73　(9)　2145-2146　2009/09

    DOI： 10.1271/bbb.90287 　

    ISSN： 0916-8451

    eISSN： 1347-6947

  52. Oxytocin receptor-deficient mice developed late-onset obesity Peer-reviewed

    Yuki Takayanagi, Yoshiyuki Kasahara, Tatsushi Onaka, Nobuyuki Takahashi, Teruo Kawada, Katsuhiko Nishimori

    NEUROREPORT　19　(9)　951-955　2008/06

    DOI： 10.1097/WNR.0b013e3283021ca9 　

    ISSN： 0959-4965

  53. Impaired thermoregulatory ability of oxytocin-deficient mice during cold-exposure Peer-reviewed

    Yoshiyuki Kasahara, Yuki Takayanagi, Teruo Kawada, Keiichi Itoi, Katsuhiko Nishimori

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　71　(12)　3122-3126　2007/12

    DOI： 10.1271/bbb.70498 　

    ISSN： 0916-8451

    eISSN： 1347-6947

Show all ︎Show first 5

Misc. 56

  1. 神経発達期におけるキヌレニン代謝産物増加が胎児に及ぼす影響 Peer-reviewed

    村上由希, 今村行雄, 笠原好之, 吉田千尋, 桃野友太, 方軻, 酒井大輔, 小西行郎, 西山利正

    Trace Nutrients Research　40　87-91　2023/10

  2. μオピオイド受容体欠損マウスにおけるδオピオイド受容体作動薬の相加効果による絶望感軽減の雌雄差

    森屋 由紀, 笠原 好之, 嶋田 政史, 榊原 泰史, 藤井 秀明, 長瀬 博, 井手 聡一郎, 池田 和隆, Hall Scott, Uhl George, 曽良 一郎

    日本神経精神薬理学会年会プログラム・抄録集　53回　173-173　2023/09

    Publisher: (一社)日本神経精神薬理学会

  3. 妊娠マウスに対する高脂肪食負荷は胎児自律神経活動を低下させる

    笠原 好之, 吉田 千尋, 木村 芳孝, 齋藤 昌利

    DOHaD研究　11　(3)　35-35　2023/08

    Publisher: (一社)日本DOHaD学会

    ISSN： 2187-2562

    eISSN： 2187-2597

  4. Evaluation of changes in autonomic nervous system activity and maternal and fetal heart rate similarity during fetal period in mouse models of autism based on DOHaD theory

    KASAHARA Yoshiyuki, KASAHARA Yoshiyuki, YOSHIDA Chihiro, WIDATALLA Namareq, UKEZONO Masatoshi, IDA Erika, ITO Ryoma, MOMONO Yuta, ISHIKAWA Ken, KIMURA Yoshitaka, KIMURA Yoshitaka, SAITO Masatoshi, SAITO Masatoshi

    11th Congress of the Asian Society for Child and Adolescent Psychiatry and Allied Professions, 2023　2023

  5. 母体免疫活性化マウスにおける神経発達障害と胎児心電図変化

    桃野 友太, 笠原 好之, 木村 芳孝, 八重樫 伸生, 齋藤 昌利

    DOHaD研究　9　(1)　36-36　2021/09

    Publisher: (一社)日本DOHaD学会

    ISSN： 2187-2562

    eISSN： 2187-2597

  6. Examination of autonomic activity and behavior during development using a mouse model of valproic acid

    請園正敏, 笠原好之

    行動科学　59　(2)　2021

    ISSN： 0919-7435

  7. 【周産期と循環管理】胎児心疾患の管理 母体腹壁誘導胎児心電図

    桃野 友太, 笠原 好之, 川滝 元良, 木村 芳孝, 八重樫 伸生, 齋藤 昌利

    周産期医学　50　(7)　1093-1097　2020/07

    Publisher: (株)東京医学社

    ISSN： 0386-9881

  8. バルプロ酸自閉症モデルマウスにおける社会的促進を用いた他個体認知

    請園正敏, 請園正敏, 笠原好之, 吉田千尋, 村上由希, 桜田一洋, 木村芳孝, 小西行郎

    日本心理学会大会発表論文集　83rd　2019

  9. μオピオイド受容体欠損マウスにおけるストレスとアルコール嗜好性に対する雌雄差の解析

    森屋 由紀, 笠原 好之, 萩野 洋子, 池田 和隆, 曽良 一郎

    日本生物学的精神医学会・日本神経精神薬理学会合同年会プログラム・抄録集　39回・47回　161-161　2017/09

    Publisher: 日本生物学的精神医学会・日本神経精神薬理学会

  10. μ-オピオイド受容体ノックアウトマウスのアルコール摂取量に及ぼす青年期ストレスの影響の性差(Sex Differences in the Effects of Adolescent Stress on Alcohol Consumption in μ-Opioid Receptor Knockout Mice)

    森屋 由紀, 笠原 好之, Hall F. Scott, Uhl George R., 曽良 一郎, 富田 博秋

    日本神経精神薬理学雑誌　36　(5-6)　123-124　2016/11

    Publisher: (一社)日本神経精神薬理学会

    ISSN： 1340-2544

  11. 胎児期オキシトシン投与時の胎児脳および成体脳における遺伝子発現変化の解析 Peer-reviewed

    鈴木 祥恵, 尾之内 勇治, 笠原 好之, 小野 千晶, 菊地 淑恵, 兪 志前, 富田 博秋

    日本神経精神薬理学会年会プログラム・抄録集　46回　224-224　2016/07

    Publisher: (一社)日本神経精神薬理学会

  12. 胎児期のオキシトシン曝露による成育後の不安行動、社会行動への影響 Peer-reviewed

    尾之内 勇治, 鈴木 祥恵, 笠原 好之, 小野 千晶, 菊地 淑恵, 兪 志前, 富田 博秋

    日本神経精神薬理学会年会プログラム・抄録集　46回　224-224　2016/07

    Publisher: (一社)日本神経精神薬理学会

  13. 恐怖記憶の持続におけるミクログリア由来TNF-αの発現(Role of microglial TNF-alpha in fear memory formation) Peer-reviewed

    兪 志前, 福島 穂高, 小野 千晶, 坂井 舞, 笠原 好之, 菊地 淑恵, Gunawansa Nicole, 高橋 雄太, 松岡 洋夫, 喜田 聡, 富田 博秋

    日本神経精神薬理学会年会プログラム・抄録集　46回　238-238　2016/07

    Publisher: (一社)日本神経精神薬理学会

  14. Methamphetamine induced changes of monoamine neurotransmission in 5-HT1B KO mice

    Yuki Moriya, Yoko Hagino, Scott F. Hall, Rene Hen, Kazutaka Ikeda, Yoshiyuki Kasahara, Ichiro Sora, George R. Uhl

    INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY　19　159-159　2016/06

    ISSN： 1461-1457

    eISSN： 1469-5111

  15. Pregnant mice exposure to extreme level of oxytocin influences emotional and social behaviors of the offsprings after growing up

    Yoshiyuki Kasahara, Sachie Suzuki, Yuji Onouchi, Ayaka Yoshida, Chiaki Ono, Yoshie Kikuchi, Zhiqian Yu, Hiroaki Tomita

    INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY　19　50-50　2016/06

    ISSN： 1461-1457

    eISSN： 1469-5111

  16. Association between mu-opioid receptor gene variant 118 A &gt; G and personality traits among Japanese population

    Yumiko Kubo, Hikaru Takeuchi, Yoshie Kikuchi, Yoshiyuki Kasahara, Zhiqian Yu, Yasuyuki Taki, Ryuta Kawashima, Hiroaki Tomita

    INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY　19　299-300　2016/06

    ISSN： 1461-1457

    eISSN： 1469-5111

  17. Region-Specific Dendritic Spine Loss of Pyramidal Neurons in Dopamine Transporter Knockout Mice

    Ichiro Sora, Yosefu Arime, Frank Hall, George R. Uhl, Yoshiyuki Kasahara

    NEUROPSYCHOPHARMACOLOGY　40　S222-S222　2015/12

    ISSN： 0893-133X

    eISSN： 1740-634X

  18. 精神刺激薬によるドパミン作動性神経のホメオスタシス破綻への調節メカニズム モノアミン神経伝達に関わる遺伝子改変マウスを用いた精神疾患研究

    笠原 好之, 久保 有美子, 森屋 由紀, 有銘 預世布, 富田 博秋, 曽良 一郎

    日本アルコール・薬物医学会雑誌　50　(4)　157-157　2015/08

    Publisher: (一社)日本アルコール・アディクション医学会

    ISSN： 1341-8963

  19. 統合失調症のニコチン依存におけるCadherin 13の役割 Peer-reviewed

    大塚郁夫, Hishimoto Akitoyo, 渡部雄一郎, 朴秀賢, 笠原好之, Ichiro Sora

    日本アルコール・薬物医学会雑誌　50　(4号)　207-207　2015/08

    Publisher: (一社)日本アルコール・アディクション医学会

    ISSN： 1341-8963

  20. 胎生期ストレスが胎児のエピゲノム変化および精神行動に及ぼす影響の特定 Peer-reviewed

    兪 志前, 舟山 亮, 植野 和子, 成相 直樹, 小島 要, 小野 千晶, 笠原 好之, 長崎 正朗, 中山 啓子, 富田 博秋

    日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集　24回・44回　204-204　2014/11

    Publisher: 日本臨床精神神経薬理学会・日本神経精神薬理学会

  21. SEX DIFFERENCES IN THE EFFECTS OF CHRONIC SOCIAL ISOLATION ON ALCOHOL CONSUMPTION IN MU-OPIOID RECEPTOR KNOCKOUT MICE

    Y. Moriya, Y. Kasahara, F. S. Hall, G. R. Uhl, I. Sora

    ALCOHOL AND ALCOHOLISM　49　2014/09

    ISSN： 0735-0414

    eISSN： 1464-3502

  22. Analysis of dopamine transporter knockout mice as an animal model of AD/HD

    Yoshiyuki Kasahara, Yumiko Kubo, Ichiro Sora

    Japanese Journal of Neuropsychopharmacology　33　(5-6)　185-189　2013/11

    ISSN： 1340-2544

  23. 中枢刺激薬作用機序から知るAD/HDの病態メカニズム.

    笠原好之, 久保有美子, 曽良一郎

    日本神経精神薬理学雑誌　33　185-189　2013/11

  24. メタンフェタミン依存に関する分子遺伝学的研究.

    森屋由紀, 笠原好之, 曽良一郎

    日本神経精神薬理学雑誌　33　155-160　2013/09

  25. Genetic vulnerability of methamphetamine dependence

    Yuki Moriya, Yoshiyuki Kasahara, Ichiro Sora

    Japanese Journal of Neuropsychopharmacology　33　(4)　155-160　2013/08

    ISSN： 1340-2544

  26. 覚せい剤精神病の分子遺伝学的機序

    曽良一郎, 山崎圭太, 森屋由紀, 笠原好之, 十川千春, 北山滋雄, 花尻瑠理, 合田幸広, 池田和隆

    厚生労働科学研究費補助金 (医薬品・医療機器等レギュラトリーサイエンス総合研究推進事業), 乱用薬物による薬物依存の発症メカニズム・予防・診断及び治療法に関する研究, 平成23年度 総括研究報告書 (研究代表者: 鍋島俊隆)　76-86　2012

  27. 【モデル動物を用いた精神疾患のメカニズム研究;脳発達の視点から】 ドーパミン神経伝達過剰マウスモデルにおける神経発達 (日本神経精神薬理学雑誌)

    笠原好之, 有銘預世布, 久保有美子, 福井麻美, 曽良一郎

    日本神経精神薬理学雑誌　31　(5月6日)　195-199　2011/11

  28. Regulation of the body temperature by oxytocin receptor/serotonin pathway

    Yuko Tateishi, Keisuke Sato, Yoshiyuki Kasahara, Hiroaki Mizukami, Katsuhiko Nishimori

    NEUROSCIENCE RESEARCH　71　E163-E163　2011

    DOI： 10.1016/j.neures.2011.07.705 　

    ISSN： 0168-0102

  29. Oxytocin receptor-deficient mice showed dysfunction of the thermoregulatory ability

    Keisuke Sato, Yoshiyuki Kasahara, Hiroaki Mizukami, Katsuhiko Nishimori

    NEUROSCIENCE RESEARCH　71　E163-E163　2011

    DOI： 10.1016/j.neures.2011.07.704 　

    ISSN： 0168-0102

  30. Methamphetamine-induced behavioral sensitization in 5-HT1B-KO mice

    Yuki Moriya, Kana Ishihara, Yoshiyuki Kasahara, Ichiro Sora

    NEUROSCIENCE RESEARCH　71　E397-E397　2011

    DOI： 10.1016/j.neures.2011.07.1741 　

    ISSN： 0168-0102

  31. 統合失調症動物モデルにおける神経回路の形成・発達の解析 (精神薬療研究年報)

    笠原好之, 有銘預世布, 福井麻美, 久保有美子, 曽良一郎

    精神薬療研究年報　(43)　73-74　2011

  32. 覚せい剤精神病の分子遺伝学的機序

    曽良一郎, 森屋由紀, 小林秀昭, 萩野洋子, 有銘預世布, 笠原好之, 糸川昌成, 岩田仲生, 稲田俊也, 山田光彦, 関根吉統, 内村直尚, 伊豫雅臣, 尾崎紀夫, 氏家寛, 池田和隆

    厚生労働科学研究費補助金 (医薬品・医療機器等レギュラトリーサイエンス総合研究推進事業), 乱用薬物による薬物依存の発症メカニズム・予防・診断及び治療法に関する研究, 平成22年度 総括研究報告書 (研究代表者: 鍋島俊隆)　68-76　2011

  33. 【薬物依存症 薬物依存症のトレンド】 薬物依存症の臨床各論 最新動向 コカイン依存研究の動向 (日本臨床)

    笠原好之, 有銘預世布, 福井麻美, 内海修, 曽良一郎

    日本臨床　68　(8)　1479-1485　2010/08

    Publisher: 日本臨床社

    ISSN： 0047-1852

  34. 脳内報酬系を担うドーパミン神経伝達 (現代のエスプリ)

    曽良一郎, 久保有美子, 有銘預世布, 笠原好之, 佐藤拓

    現代のエスプリ　513　124-133　2010/04

  35. 【アトモキセチン 基礎と臨床】 アトモキセチンの薬理作用機序 (精神科)

    有銘預世布, 福井麻美, 笠原好之, 曽良一郎

    精神科　16　(3)　223-226　2010/03

    Publisher: 科学評論社

    ISSN： 1347-4790

  36. The Necdin knockout mice, a pathogenically model animal of Prader-Willi syndrome, showed dysfunction in the thermoregulation

    Maki Sasaki, Hiroki Hamada, Keisuke Sato, Yoshiyuki Kasahara, Kazuaki Yoshikawa, Katsuhiko Nishimori

    NEUROSCIENCE RESEARCH　68　E416-E416　2010

    ISSN： 0168-0102

  37. モデル動物を用いた精神疾患のメカニズム研究 脳発達の視点から ドーパミン神経伝達過剰マウスモデルにおける神経発達 (日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集)

    笠原 好之, 有銘 預世布, 久保 有美子, 福井 麻美, 曽良 一郎

    日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集　20回・40回　52-52　2010

  38. ドーパミントランスポーターノックアウトマウスにおける前頭葉の神経可塑性と認知機能障害 (日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集)

    有銘 預世布, 笠原 好之, 曽良 一郎

    日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集　20回・40回　152-152　2010

  39. 若齢AD/HD動物モデルにおける治療薬反応性 (日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集)

    久保 有美子, 有銘 預世布, 笠原 好之, 曽良 一郎

    日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集　20回・40回　192-192　2010

  40. [Trend in the study of cocaine addiction]. (Nihon Rinsho)

    Kasahara Yoshiyuki, Arime Yosefu, Fukui Asami, Uchiumi Osamu, Sora Ichiro

    Nihon Rinsho　68　(8)　1479-1485　2010

    ISSN： 0047-1852

  41. メタンフェタミン依存へのセロトニン1B、アデノシンA2受容体の関与

    曽良一郎, 小林秀昭, 石原佳奈, 有銘預世布, 萩野洋子, 池田和隆, 糸川昌成, 岩田仲生, 稲田俊也, 山田光彦, 関根吉統, 内村直尚, 伊豫雅臣, 尾崎紀夫, 氏家寛, 笠原好之

    厚生労働科学研究費補助金 (医薬品・医療機器等レギュラトリーサイエンス総合研究推進事業), 乱用薬物による神経毒性・依存症に対する診断・予防及び治療法に関する研究, 平成21年度総括研究報告書 (主任研究者: 鍋島俊隆)　56-66　2010

  42. 依存性薬物の精神神経毒性と標的分子への作用機序の解明

    曽良一郎, 笠原好之, 小林秀昭, 李炳錦, 石原佳奈, 猪狩もえ, 萩野洋子, SHEN Haowei, 山本秀子, 有銘預世布, 十川千春, 十川紀夫, 糸川昌成, 岩田仲生, 稲田俊也, 山田光彦, 関根吉統, 内村直尚, 伊豫雅臣, 尾崎紀夫, 氏家寛, 北山滋雄, 池田和隆

    厚生労働科学研究費補助金 (医薬品・医療機器等レギュラトリーサイエンス総合研究推進事業), 乱用薬物による神経毒性・依存症に対する診断・予防及び治療法に関する研究, 平成19年度～平成21年度3年間のまとめ・総合研究報告書 (主任研究者: 鍋島俊隆)　190-204　2010

  43. 【Atomoxetineの導入でAD/HD治療はどう変わるか?】 Atomoxetineのプロフィールと薬理作用 (臨床精神薬理)

    曽良一郎, 福井麻美, 池田和隆, 笠原好之

    臨床精神薬理　12　(9)　1951-1956　2009/09

  44. 【発達障害の新たな遺伝子】 AD/HDの遺伝要因解明の現状 (分子精神医学)

    曽良一郎, 笠原好之, 内海修, 久保有美子, 富田博秋, 池田和隆

    分子精神医学　9　(3)　262-267　2009/07

    Publisher: 先端医学社

    ISSN： 1345-9082

  45. Generation of AAV vector expressing oxytocin receptor to study the rescue experiments with oxytocin receptor knockout mice

    Keisuke Sato, Daichi Osada, Yumi Aoyagi, Yoshiyuki Kasahara, Hiroaki Mizukami, Shizu Hidema, Keiya Ozawa, Larry J. Young, Katsuhiko Nishimori

    NEUROSCIENCE RESEARCH　65　S242-S242　2009

    DOI： 10.1016/j.neures.2009.09.1371 　

    ISSN： 0168-0102

  46. Prefrontal NET blockade ameliorates prepulse inhibition deficits in dopamine transporter knockout mice

    Yosefu Arime, Yoshiyuki Kasahara, Ichiro Sora

    NEUROSCIENCE RESEARCH　65　S252-S252　2009

    DOI： 10.1016/j.neures.2009.09.1436 　

    ISSN： 0168-0102

  47. メタンフェタミン行動感作形成におけるセロトニン神経伝達の関与

    曽良一郎, 猪狩もえ, 萩野洋子, SHEN Haowei, 石原佳奈, 山本秀子, 有銘預世布, 笠原好之, 池田和隆

    厚生労働科学研究費補助金 (医薬品・医療機器等レギュラトリーサイエンス総合研究推進事業), 乱用薬物による神経毒性・依存症に対する診断・予防及び治療法に関する研究, 平成20年度研究報告書 (主任研究者: 鍋島俊隆)　216-224　2009

  48. MONOAMINE TRANSPORTER AS A TARGET MOLECULE FOR PSYCHOSTIMULANTS

    Ichiro Sora, BingJin Li, Setsu Fumushima, Asami Fukui, Yosefu Arime, Yoshiyuki Kasahara, Hiroaki Tomita, Kazutaka Ikeda

    ADVANCES IN NEUROPHARMACOLOGY　85　29-33　2009

    DOI： 10.1016/S0074-7742(09)85003-4 　

    ISSN： 0074-7742

  49. 【中枢神経系のトランスポーターをめぐって】 トランスポーターの遺伝子改変動物と精神神経疾患 (Clinical Neuroscience)

    曽良一郎, 笠原好之

    Clinical Neuroscience　26　(10)　1081-1083　2008/10

  50. オキシトシンと情動

    西森克彦, 吉田匡秀, 笠原好之, 高柳友紀

    分子精神医学　8　(3)　25-33　2008/07/10

    Publisher: 先端医学社

  51. 【神経ペプチド】 オキシトシンと情動 (分子精神医学)

    西森克彦, 吉田匡秀, 笠原好之, 高柳友紀

    分子精神医学　8　(3)　221-229　2008/07

    Publisher: 先端医学社

    ISSN： 1345-9082

  52. New aspects of oxytocin receptor function revealed by knockout mice: sociosexual behaviour and control of energy balance

    Katsuhiko Nishimori, Yuki Takayanagi, Masahide Yoshida, Yoshiyuki Kasahara, Larry J. Young, Masaki Kawamata

    ADVANCES IN VASOPRESSIN AND OXYTOCIN: FROM GENES TO BEHAVIOUR TO DISEASE　170　79-90　2008

    DOI： 10.1016/S0079-6123(08)00408-1 　

    ISSN： 0079-6123

  53. オキシトシン受容体遺伝子の生理機能 本能行動制御から体恒常性維持 (化学と生物)

    西森克彦, 高柳友紀, 吉田匡秀, 笠原好之

    化学と生物　45　(12)　826-833　2007/12

    Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry

    DOI： 10.1271/kagakutoseibutsu1962.45.826 　

    ISSN： 0453-073X

  54. Cold-sensitive phenotype in mice lacking oxytocin receptor

    Yoshiyuki Kasahara, Yuki Takayanagi, Masahide Yoshida, Teruo Kawada, Keiichi Itoi, Katsuhiko Nishimori

    NEUROSCIENCE RESEARCH　58　S220-S220　2007

    ISSN： 0168-0102

  55. Oxytocin receptor gene deficient mice showed multiple disruptions in sociosexual behavior

    Katsuhiko Nishimori, Yuki Takayanagi, Masahide Yoshida, Yoshiyuki Kasahara, Masaki Kawamata

    NEUROSCIENCE RESEARCH　55　S28-S28　2006

    ISSN： 0168-0102

  56. THE ROLE OF CENTRAL NORADRENERGIC (NA) SYSTEM IN NEUROENDOCRINE RESPONSES FOLLOWING INTRAPERITONEAL ADMINISTRATION OF LIPOPOLYSACCHARIDE (LPS)

    OTAKI Ikue, YASOSIMA Yasunobu, NAKAMURA Hiroaki, KASAHARA Yoshiyuki, SUZUKI Saya, KOBAYASHI Kazuto, ITOI Keiichi

    (20)　41-41　2005/11/12

    ISSN： 0913-9036

Show all ︎Show first 5

Books and Other Publications 1

  1. 実験薬理学 実践行動薬理学

    社団法人日本薬理学会, ed, 曽良一郎, 石原佳奈, 笠原好之, 山本秀子, 池田和隆

    金芳堂　2010/03

Presentations 78

  1. 妊娠マウスに対する高脂肪食負荷は胎児自律神経活動を低下させる

    笠原好之, 吉田千尋, 木村芳孝, 齋藤昌利

    第11回日本DOHaD学会学術集会　2023/08/05

  2. 自閉症モデルマウスの胎児期における自律神経活動変化と母体と胎児の心拍類似性の評価 Invited

    笠原好之, 吉田千尋, ナマレックウィダタラ, 木村芳孝, 齋藤昌利

    2023年度北海道実験動物研究会大会（HALAS 2023）　2023/07/09

  3. Evaluation of changes in autonomic nervous system activity and maternal and fetal heart rate similarity during fetal period in mouse models of autism based on DOHaD theory. Invited

    Kasahara Y, Yoshida C, Widatalla N, Ukezono M, Ida E, Ito R, Momono Y, Ishikawa K, Kimura Y, Saito M

    The 11th Congress of The Asian Society for Child and Adolescent Psychiatry and Allied Professions (ASCAPAP 2023)　2023/05/26

  4. 発達障害研究における胎児心電図計測の有用性 Invited

    笠原好之, 木村芳孝

    日本発達神経科学学会第6回大会　2017/11/25

  5. 精神疾患動物モデルとしてのドーパミン神経伝達変異マウス Invited

    笠原好之, 久保有美子, 有銘預世布, 曽良一郎

    第60回日本神経化学大会　2017/09/07

  6. モノアミン神経伝達に関わる遺伝子改変マウスを用いた精神疾患研究 Invited

    笠原好之, 久保有美子, 森屋由紀, 有銘預世布, 曽良一郎

    平成27年度アルコール・薬物依存関連学会合同学術総会　2015/10/12

  7. AD/HD動物モデルで見られる領域特異的なスパイン減少

    笠原好之

    新学術領域「マイクロ精神病態」若手交流研究会　2014/02/13

  8. AD/HD動物モデルの若齢期における治療薬応答性と分子神経基盤の解明

    久保有美子, 笠原好之, 有銘預世布, 富田博秋, 曽良一郎

    東北大学大学院医学系研究科 第7回リトリート 大学院生研究発表会　2014/01/18

  9. Methamphetamine induced changes of monoamine neurotransmission in 5-HT1B KO mice. International-presentation

    Moriya Y, Kasahara Y, Hagino Y, Hall FS, Hen R, Ikeda K, Uhl GR, Sora I

    11th World Congress of Biological Psychiatry　2013/06/23

  10. セロトニントランスポーター欠損マウスにおける不安関連行動および認知柔軟性の発達

    榊原泰史, 笠原好之, 曽良一郎

    第36回日本神経科学大会　2013/06/20

  11. 注意欠陥多動性障害における発達期依存的治療薬反応性の機序

    笠原好之

    第2回東北脳科学ウィンタースクール　2013/02/23

  12. 依存性薬物の作用機序におけるセロトニン神経伝達の機能解析

    森屋由紀, 笠原好之, 曽良一郎

    東北大学大学院医学系研究科 第6回リトリート 大学院生研究発表会　2013/01/19

  13. Different Behavioral Profile at Developmental Stages in Serotonin Transporter Knockout Mice International-presentation

    Sakakibara Y, Kasahara Y, Sora I

    JSPS-NRF Asian Science Seminar 2012　2012/02/13

  14. Society for Neuroscience (SfN) 報告会

    笠原好之

    第39回東北大学脳科学GCOE 第6回脳科学若手の会東北部会 若手フォーラム　2011/11/25

  15. Additive suppressive effects of δ-opioid agonist pretreatment and μ-opioid receptor knockout on responses to forced swim stress. International-presentation

    Kasahara Y, Shimada M, Sasaki K, Ide S, Komatsu H, Ikeda K, Hall FS, Uhl GR, Nagase H, Sora I

    The Society of Neuroscience (SFN) 41th Annual Meeting　2011/11/12

  16. The impairment of synaptic formation in dopamine transporter knockout mice. International-presentation

    Fukui A, Kasahara Y, Arime Y, Hall FS, Uhl GR, Sora I

    The Society of Neuroscience (SFN) 41th Annual Meeting　2011/11/12

  17. Age dependent drug responses in DAT KO mice as an animal model of ADHD. International-presentation

    Kubo Y, Kasahara Y, Arime Y, Hall FS, Ikeda K, Uhl GR, Sora I

    The Society of Neuroscience (SFN) 41th Annual Meeting　2011/11/12

  18. Altered methamphetamine-induced increases in extracellular monoamine levels in 5-HT1B knockout mice. International-presentation

    Moriya Y, Kasahara Y, Hagino Y, Ishihara K, Hall FS, Hen R, Ikeda K, Uhl GR, Sora I

    The Society of Neuroscience (SFN) 41th Annual Meeting　2011/11/12

  19. Serotonergic regulation on the recovery of behavioral abnormalities with nicotine treatment in DAT KO mice. International-presentation

    Uchiumi O, Kasahara Y, Fukui A, Hall FS, Uhl GR, Sora I

    The Society of Neuroscience (SFN) 41th Annual Meeting　2011/11/12

  20. Oxytocin receptor-deficient mice showed dysfunction of the thermoregulatory ability caused by the absence of expression in the hypothalamus. International-presentation

    Sato K, Kasahara Y, Tateishi Y, Mizukami H, Nishimori K

    The Society of Neuroscience (SFN) 41th Annual Meeting　2011/11/12

  21. 5-HT1B受容体KOマウスにおけるメタンフェタミン誘発性行動感作.

    森屋由紀, 笠原好之, 萩野洋子, 有銘預世布, 池田和隆, 曽良一郎

    第21回日本臨床精神神経薬理学会・第41回日本神経精神薬理学会 合同年会　2011/10/27

  22. ドーパミントランスポーター（DAT）欠損マウス前頭前野皮質における神経発達不全.

    福井麻美, 笠原好之, 有銘預世布, 曽良一郎

    第21回日本臨床精神神経薬理学会・第41回日本神経精神薬理学会 合同年会　2011/10/27

  23. ストレス応答に対するμおよびδオピオイド受容体の関与.

    嶋田政史, 笠原好之, 佐々木一益, 井手聡一郎, 小松浩, 池田和隆, 長瀬博, 曽良一郎

    第21回日本臨床精神神経薬理学会・第41回日本神経精神薬理学会 合同年会　2011/10/27

  24. Novel δ opioid receptor agonist KNT-127 reduced immobility time in μ opioid receptor knockout mouse in forced swim stress test. International-presentation

    Shimada M, Kasahara Y, Sasaki K, Ide S, Komatsu H, Hall FS, Uhl GR, Nagase H, Sora I

    2nd Congress of Asian College of Neuropsychopharmacology (AsCNP)　2011/09/23

  25. Methamphetamine-induced behavioral sensitization in 5-HT1B-KO mice.

    森屋由紀, 石原佳奈, 笠原好之, 曽良一郎

    第34回日本神経科学大会　2011/09/14

  26. Analysis of function in body temperature regulation by oxytocin receptor/serotonin system.

    立石木綿子, 佐藤佳亮, 笠原好之, 水上浩明, 西森克彦

    第34回日本神経科学大会　2011/09/14

  27. Oxytocin receptor-deficient mice showed dysfunction of the thermoregulatory ability.

    佐藤佳亮, 笠原好之, 水上浩明, 西森克彦

    第34回日本神経科学大会　2011/09/14

  28. The Necdin (Ndn)-deficient mice, reminiscent of the human Prader-Willi syndrome (PWS), showed multiple-impairment related to the hypothalamic nucleus International-presentation

    Sasaki M, Sato K, Kasahara Y, Hamada H, Itoi K, Yoshikawa K, Nishimori K

    9th World Congress on Neurohypophysial Hormones　2011/07/27

  29. 遺伝性疾患Prader-Willi syndrome (PWS) モデルNdn変異マウスを用いたOXT/OXTRとの関連性

    佐々木真紀, 佐藤佳亮, 笠原好之, 濱田祥紀, 井樋慶一, 吉川和明, 西森克彦

    第15回日本行動神経内分泌研究会　2011/06/30

  30. Modulation of behavioral responses to stress by opioid systems. International-presentation

    Sasaki K, Shimada M, Kasahara Y, Ide S, Komatsu H, Hall FS, Uhl GR, Nagase H, Sora I

    The International Narcotics Research Conference 2011　2011/06/21

  31. 神経精神疾患動物モデルとしてのモノアミントランスポーター欠損マウス.

    笠原好之, 有銘預世布, 久保有美子, 内海修, 福井麻美, 森屋由紀, 曽良一郎

    第6回トランスポーター研究会年会　2011/06/11

  32. Impaired Thermoregulation in the Necdin (Ndn) Knockout Mice

    佐々木真紀, 佐藤佳亮, 笠原好之, 井樋慶一, 吉川和明, 西森克彦

    第88回日本生理学会大会, 第116回日本解剖学会総会・全国学術集会 合同大会　2011/03/28

  33. Serotonergic regulation on 1 mg/kg of methamphetamine-induced behavioral sensitization. International-presentation

    Igari M, Shen HW, Hagino Y, Fukushima S, Kasahara Y, Lesch KP, Murphy DL, Hall FS, Uhl GR, Ikeda K, Yaegashi N, Sora I

    The 1st Tohoku International Symposium on Multidisciplinary Neuroscience　2011/01/21

  34. Analysis of body thermoregulation function by oxytocin receptor / serotonin system. International-presentation

    Tateishi Y, Sato K, Kasahara Y, Mizukami H, Nishimori K

    The 1st Tohoku International Symposium on Multidisciplinary Neuroscience　2011/01/21

  35. Recovery of PPI deficits in DAT KO mice by treatment with nicotine. International-presentation

    Kasahara Y, Uchiumi O, Arime Y, Hall FS, Uhl GR, Sora I

    The Society of Neuroscience (SFN) 40th Annual Meeting　2010/11/13

  36. ドーパミントランスポーターノックアウトマウスにおける前頭葉の神経可塑性と認知機能障害

    有銘預世布, 笠原好之, 曽良一郎

    第20回日本臨床精神神経薬理学会・第40回日本神経精神薬理学会 合同年会　2010/09/15

  37. 若齢AD/HD動物モデルにおける治療薬反応性

    久保有美子, 有銘預世布, 笠原好之, 曽良一郎

    第20回日本臨床精神神経薬理学会・第40回日本神経精神薬理学会 合同年会　2010/09/15

  38. ドーパミン神経伝達過剰マウスモデルにおける神経発達

    笠原好之, 有銘預世布, 久保有美子, 福井麻美, 曽良一郎

    第20回日本臨床精神神経薬理学会・第40回日本神経精神薬理学会 合同年会　2010/09/15

  39. Prader-Willi syndromeモデルNecdin欠損マウスを用いたオキシトシンシステムとの関連

    佐々木真紀, 濱田祥紀, 佐藤佳亮, 笠原好之, 吉川和明, 西森克彦

    第13回日本行動神経内分泌研究会　2010/09/13

  40. Prader-Willi syndromeモデルのNecdin遺伝子欠損マウスは体温調節能に異常を呈する

    佐々木真紀, 濱田祥紀, 佐藤佳亮, 笠原好之, 吉川和明, 西森克彦

    Neuro2010 第33回日本神経科学大会 第53回日本神経化学会大会 第20回日本神経回路学会大会　2010/09/02

  41. MEETING REPORTS in 台湾

    笠原好之

    第22回脳科学GCOE若手フォーラム　2010/01/27

  42. DOPAMINE TRANSPORTER KNOCKOUT MICE AS A HYPERDOPAMINERGIC ANIMAL MODEL OF SCHIZOPHRENIA. International-presentation

    Kasahara Y, Arime Y, Kubo Y, Li B, Sora I

    Tohoku University - Taiwan Neuroscience Workshop for Young Scientists　2010/01/21

  43. メタンフェタミンによる行動感作へのセロトニン1B受容体の関与

    石原佳奈, 猪狩もえ, 笠原好之, 曽良一郎

    第39回日本神経精神薬理学会・第19回日本臨床精神神経薬理学会合同大会　2009/11/13

  44. The selective norepinephrine transporter blocker nisoxetine attenuates deficits of prepulse inhibition in mice after administration of the NMDA antagonist MK-801. International-presentation

    Arime Y, Kasahara Y, Hall FS, Uhl GR, Sora I

    The Society for Neuroscience (SFN) 39th Annual Meeting　2009/10/17

  45. オキシトシン受容体発現AAV-Oxtrベクターによる生理機能制御領域の解明

    佐藤佳亮, 長田大知, 青?有美, 笠原好之, 水上浩明, 日出間志寿, 小澤敬也, Larry J. Young, 西森克彦

    第32回日本神経科学大会　2009/09/16

  46. 前頭前野のNET阻害によるDAT欠損マウスのPPI改善効果

    有銘預世布, 笠原好之, 曽良一郎

    第32回日本神経科学大会　2009/09/16

  47. Impaired thermoregulatory ability of oxytocin and oxytocin receptor deficient mice during cold-exposure International-presentation

    Kasahara Y, Sato K, Takayanagi Y, Kawada T, Itoi K, Nishimori K

    VIIIth World Congress on Neurohypophysial Hormones　2009/09/04

  48. Glutamatergic neurotransmission modulated with norepinephrine reuptake inhibitor in animal models of schizophrenia International-presentation

    Arime Y, Kasahara Y, Sora I

    The 2nd Brain Science Summer Retreat　2009/07/25

  49. オキシトシン受容体発現Oxtr-AAVベクターの開発と生理機能制御領域の解明

    佐藤佳亮, 青柳有美, 長田大知, 笠原好之, 水上浩明, 日出間志寿, 西森克彦

    日本農芸化学会2009年度大会　2009/03/27

  50. オキシトシン受容体発現Oxtr-AAVベクターの開発

    佐藤佳亮, 笠原好之, 青柳有美, 水上浩明, 小澤敬也, 日出間志寿, Young J. Larry, 西森克彦

    第31回日本分子生物学会年会・第81回日本生化学会大会合同大会　2008/12/09

  51. Reduced brain-derived neurotrophic factor in prefrontal cortex of dopamine transporter knockout mice. International-presentation

    Kasahara Yoshiyuki, Li Bingjin, Arime Yosefu, Yamashita Motoyasu, Sora Ichiro

    Tohoku University-Fudan University Neuroscience Workshop for Young Scientists　2008/10/15

  52. ドーパミン神経伝達過剰マウスモデルにおける前頭前野皮質BDNFの低下

    笠原好之, 李炳錦, 有銘預世布, 山下元康, 曽良一郎

    東北CNSフォーラム　2008/10/11

  53. Oxytocin/Oxytocin receptor system controls social behaviors and body temperature International-presentation

    Katsuhiko Nishimori, Masahide Yoshida, Yoshiyuki Kasahara

    International Symposium on Biologically Active Peptides: Peptide Diversity　2008/08/31

  54. オキシトシン受容体の生理機能解析を目指したAAV-Oxtrベクターの開発と応用

    佐藤佳亮, 笠原好之, 青柳有美, 水上浩明, 小澤敬也, 日出間志寿, 西森克彦

    第7回行動神経内分泌研究会　2008/07/26

  55. 遺伝子欠損マウスを用いたオキシトシン／オキシトシン受容体の体温調節機構に関する研究

    佐藤佳亮, 笠原好之, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    日本農芸化学会2008年度大会　2008/03/26

  56. 体温制御システムと社会行動制御に働くoxytocin/oxytocin受容体系

    西森 克彦, 笠原 好之, 吉田 匡秀, 高柳 友紀, 井樋 慶一, 吉川 和明, 尾仲 達史

    第85回日本生理学会大会　2008/03/25

  57. IMPAIRED THERMOREGULATORY ABILITY OF OXYTOCIN AND OXYTOCIN RECEPTOR DEFICIENT MICE DURING COLD-EXPOSURE

    笠原好之, 佐藤佳亮, 小野擁子, 青?有美, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    The 1st International Conference of Tohoku Neuroscience GCOE　2008/01/23

  58. Study of Physiological and Behavioral Functions of Oxytocin Receptor with Mice Whose OXTR genes were Modified International-presentation

    Nishimori K, Kasahara Y, Yoshida M, Takayanagi Y

    World Congress of Neurohypophysial Hormones 2007　2007/09/18

  59. 遺伝子欠損マウスを用いたオキシトシン受容体の体温調節機構に関する解析

    笠原好之, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    Neuro2007　2007/09/10

  60. OXT受容体遺伝子と中枢性発熱制御

    笠原好之

    第4回行動神経内分泌研究会　2007/07/07

  61. 遺伝子欠損マウスを用いたオキシトシン/オキシトシン受容体の体温調節機構に関する研究

    笠原好之, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    日本生化学会東北支部第73回例会・シンポジウム　2007/05/12

  62. 体温調節機構におけるOXT/OXTR系の役割

    笠原好之, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    第33回日本神経内分泌学会　2006/10/27

  63. オキシトシン受容体遺伝子欠損マウスは様々な社会行動異常をみせる

    西森克彦, 高柳友紀, 吉田匡秀, 笠原好之, 川又理樹

    第２９回日本神経科学大会　2006/07/19

  64. Cold-sensitive phenotype in mice lacking oxytocin receptor gene. International-presentation

    笠原好之, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    20th International Congress of Biochemistry and Molecular Biology　2006/06/18

  65. オキシトシン受容体遺伝子欠損マウスは熱産生能が低下する

    笠原好之, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    第83回日本生理学会大会　2006/03/28

  66. Molecular genetic analysis of oxytocin receptor gene function on thermoregulation

    笠原好之, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    第78回日本生化学会大会　2005/10/19

  67. 免疫？神経内分泌応答におけるオキシトシン受容体（OTR）の機能解析？OTR遺伝子欠損マウスを用いた検討

    笠原好之, 高柳友紀, 西森克彦, 井樋慶一

    第32回日本神経内分学会　2005/07/07

  68. 遺伝子欠損マウスにより生体でのオキシトシン受容体機能を探る

    西森克彦, 高柳友紀, 吉田匡秀, 笠原好之, 尾仲達史, 木村正, Larry J. Young

    第32回日本神経内分学会　2005/07/07

  69. Exploring the functions of the oxytocin receptor (OTR) in mediating hypothalamic neuroendocrine responses following an immuno challenge.

    笠原好之, 高柳友紀, 西森克彦, 井樋慶一

    第82回日本生理学会大会　2005/05/18

  70. オキシトシン受容体遺伝子欠損マウスの体温調節能についての解析

    笠原好之, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    第二回東北大学バイオサイエンスシンポジウム　2005/05/16

  71. 遺伝子欠損マウスを用いたオキシトシン受容体の体温調節機能に関する解析

    笠原好之, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    第10回日本内分泌学会東北地方会　2005/04/23

  72. オキシトシン受容体遺伝子欠損マウスの体温調節能についての解析

    笠原好之, 高柳友紀, 河田照雄, 井樋慶一, 西森克彦

    日本農芸化学会2005年度大会　2005/03/28

  73. Oxytocin receptor (OTR)-deficient mice are obese and cold-sensitive

    笠原好之, 高柳友紀, 河田照雄, 西森克彦

    第77回日本生化学会大会　2004/10/13

  74. オキシトシン受容体遺伝子欠損マウスの肥満病態についての解析

    笠原好之, 高柳友紀, 川又理樹, 吉田匡秀, 河田照雄, 西森克彦

    日本生化学会東北支部会第70回例会　2004/05/28

  75. オキシトシン受容体遺伝子欠損マウスの解析

    高柳友紀, 川又理樹, 笠原好之, 吉田匡秀, Larry J. Young, 木村正, 杉本幸彦, 河田照雄, 柳沢輝行, 西森克彦

    第1回東北大学バイオサイエンスシンポジウム　2004/05/14

  76. オキシトシン受容体（OTR）遺伝子欠損マウスの体温調節能についての解析

    笠原好之, 高柳友紀, 河田照雄, 西森克彦

    日本農芸化学会2004年度大会　2004/03/29

  77. オキシトシン受容体(OTR)遺伝子欠損マウスの肥満についての解析

    高柳友紀, 笠原好之, 河田照雄, 西森克彦

    第26回日本分子生物学会年会　2003/12/10

  78. オキシトシン受容体(OTR)遺伝子欠損マウスにおける肥満傾向についての解析

    高柳友紀, 笠原好之, 河田照雄, 西森克彦

    日本農芸化学会2003年度大会　2003/03/30

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Research Projects 13

  1. 母体環境の変化による胎児期の自律神経機能異常は将来の疾患リスクとなるか？

    笠原 好之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2025/04/01 - 2028/03/31

  2. 参照系AI技術を応用した痙攣性発声障害診断システムとチタンブリッジ手術支援器機開発に関する研究

    讃岐 徹治

    Offer Organization: 国立研究開発法人日本医療研究開発機構

    System: 日本医療研究開発機構研究費難治性疾患等実用化研究事業

    2024/04 - 2027/03

  3. Building a voice disorder diagnosis system based on clinical information and original AI technology

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Nagoya City University

    2024/04 - 2027/03

  4. Impact of taking parental leave on father-child bonding, well-being, and development of parental involvement education during parental leave.

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Kansai University of International Studies

    2023/04 - 2027/03

  5. 恒常性の理解と制御による糖尿病および併発疾患の克服

    片桐 秀樹

    Offer Organization: 科学技術振興機構

    System: 戦略的な研究開発の推進 ムーンショット型研究開発事業

    Institution: 東北大学

    2020 - 2025

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    AI・数理モデル解析などを活用して、代謝・循環の調節に重要である自律神経を介した臓器間ネットワークの機序を包括的に解明し、その制御手法を開発し、未病期段階の状態をより精密に検出します。それにより、2050年には、糖尿病および併発疾患の発症を未然に防ぐ社会の実現を目指します。

  6. 齧歯類における意図理解に関連した神経基盤の探索研究

    高野 裕治, 笠原 好之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    2020/04 - 2024/03

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    本課題では、人行動のモデル動物であるラット・マウスを用いて意図理解の神経基盤を解明することを目的としている。このために、霊長類で発展してきたミラーニューロンを記録するための行動実験をげっ歯類版を用いて、神経活動記録を試みるという全体計画である。初年度は、ミラーニューロン課題のマウス版を研究代表による事前準備であったラット版を参考にして開発研究に主として取り組んだ。以前のラット版は霊長類の実験場面を参考に構成されていたため、意図理解研究をげっ歯類にひらくという意味で萌芽的な研究であった。このような研究背景に基づいて、ラット同様に、マウスにもリーチングで餌報酬を獲得させることを訓練して、その後に他個体が同じ課題に従事するのを観察させるという課題構築に取り組んだ。この課題において、リーチングの事前学習がないと、マウスは他個体のリーチングを観察することが少ないことも確認することができた。そして、これらの成果を取りまとめて、マウスによる意図理解行動場面についての行動実験開発として、Behavioural Brain Res誌に公刊した。加えて、このマウスの意図理解行動は、研究代表らによる新規な行動実験系であるため、その全データを公開するためのデータ論文についても、Data in Brief誌に公刊することができた。以上の初年度までの研究活動において、意図理解の神経基盤解明研究の対象を霊長類から、げっ歯類に拡張することができたといえ、実験心理学および行動神経科学の発展に貢献することができた。加えて、開発した行動実験課題において、神経活動を記録するための計測設備の準備に取り組むことができた。

  7. 可視化された感情データの夫婦間共有による相互理解と乳児への応用

    中村 康香, 川尻 舞衣子, 吉沢 豊予子, 笠原 好之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2020/07 - 2023/03

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    今年度は、研究目的にある妊娠期における母児を対象として、心拍データを用いた感情や同調の検証に向けたデータ測定を行った。COVID-19の影響により、研究対象者のリクルートおよびデータ測定の開始時期の遅延はあったものの、開始後はスムーズに進み、現在までに予定症例数は全70例のうちの9割程が終了したところである。 母子2者間の心拍変動は、本学で開発された腹壁誘導胎児心電図(アイリスモニタ)を用いて、妊娠24～26週、30～32週、36～38週の3時点における妊婦と胎児の心電図を縦断的に測定した。同時に、妊婦の心理的状態の客観的指標を得るために唾液検体を採取し、胎児への愛着やストレスに関連する内分泌ホルモン(オキシトシン、コルチゾル、アミラーゼ)の値を測定した。妊婦と胎児の心電図データは随時、R波とR波のピークを抽出してRRI(R-R Interval)を確定するための下処理を行い、その過程を経たものから時系列解析・周波数解析を進めている。また、各ホルモン値は一定数確保できた段階で解析を外部委託し、得られた値より、妊娠経過における変化や個人内・個人間変動などを検討している。 今後は、母児の心拍変動の同調や交感神経系の評価を行った上で、妊婦の心理的状態のホルモン値の評価も併せて総合的に解析しながら母児の相互作用について検証する予定である。さらに産後を見通して、妊娠期の母児だけの関係にとどまらず、夫も含めた夫婦間の感情における追究を進める。そしてこの妊娠期の評価方法を基盤として、産後は新生児を含めた3者の相互作用・感情理解を検証し、家族が抱える様々なストレスや負担の理解、軽減され夫婦がともに育児に取り組めるための支援について検証したいと考えている。

  8. 母体免疫活性化による胎児発達と生後予後に関する生理学的特徴の抽出

    笠原 好之, 高野 裕治, 請園 正敏

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2020/04 - 2023/03

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    妊娠期の炎症による母体免疫の活性化（MIA）が胎児発達に与える影響を検討するために、ハイドロダイナミクス法による妊娠期サイトカイン高発現モデルを用いて、胎児状態を胎児心電図の計測によって検討した。前年度までに、ハイドロダイナミクス法を用いて妊娠12.5日の母体にインターロイキン17a（IL-17a）を高発現させると、胎児の自律神経の活動性が変化することを、胎児心電図の計測から明らかにした。同様の手法で妊娠期に母体のIL-17aを高発現させ、その産子マウスが成育後に社会性の障害や探索行動の変化を示すことを確認し、母体におけるIL-17aの高発現は胎児の将来に対して、自閉症スペクトラム障害（ASD）様の行動障害を引き起こすことを見出した。一方で、妊娠期のウイルス感染や細菌感染を模したMIAモデルを作成し、同様に胎児心電図計測によって胎児状態を検証した。妊娠12.5日の雌マウスに対してウイルス感染を模したPoly ICあるいは細菌感染を模したLPSを投与し、胎児の自律神経の活動性を胎児心電図計測によって評価したところ、母体Poly IC投与群、および母体LPS投与群の双方において、胎児の自律神経の活動性が変化していた。したがって、胎児期の自律神経系の活動性の変化は、MIA時における特徴のひとつであると考えられ、MIAによって引き起こされるASDなどの発症メカニズムの一端である可能性がある。現在は遺伝子解析、行動解析、組織解析などと組み合わせ、MIA時の変化の詳細とASD病態の機序の解明を進めている。

  9. 胎児心電図を用いた次世代胎児モニタリング診断技術の研究

    木村 芳孝

    Offer Organization: 国立研究開発法人日本医療研究開発機構

    System: 日本医療研究開発機構研究費成育疾患克服等総合研究事業

    2019 - 2021

  10. 基礎と臨床を橋渡しするヒト血清と培養細胞を用いた新たな漢方薬理研究法の開発

    沼田 健裕, 大場 浩史, 高山 真, 菊地 章子, 金子 聡一郎, 笠原 好之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(C)

    Institution: 東北大学

    2016/04 - 2020/03

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    本研究課題の最終的な目的は、臨床研究においてヒトに漢方薬を投与し効果を検証する一方で、細胞実験や動物実験といった基礎研究を並行しておこなうことで、基礎と臨床の橋渡しとなる新たな実験モデルの開発を行うことであった。 細胞実験は臨床研究から得られた血液を用いる計画であったが、被験者募集以前に臨床研究を中止した。そのため、臨床研究と細胞実験から明らかになった研究実績はない。 一方、動物実験については順調に遂行できたためその実績概要を以下に示す。アルコール摂取に対する黄連解毒湯の効果を評価する目的で、C57BL6/J雄マウスに対して、黄連解毒湯を経口投与した1時間後にアルコールを経口投与し、アルコール投与後30分および1時間後における血中アルコールの濃度の測定を行った。アルコールは8％濃度と15％濃度の両方を評価した。黄連解毒湯の対照群には水を同量投与した。本検討の結果、黄連解毒湯の投与によっては血中アルコール濃度には変化は見られず、本検討では効果を見出すことは出来ないという結果が得られた。

  11. Examining feasibility of brain MRI as an objective diagnostic biomarker for glaucoma

    Tatewaki Yasuko, Nakazawa Toru, Omodaka Kazuko, Tomita Hiroaki, Ono Chiaki, Kasahara Yoshiyuki, Matsudaira Izumi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2014/04 - 2018/03

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    We have been conducted longitudinal data aquisition and have analyzed cross-sectional data including mainly anatomical 3D-T1WI images and clinical visual field information, MD(mean deviation). Voxel-based regressing analysis with SPM12(Statistical Parametric Mapping software 12) revealed that gray matter volume of the specific region in primary visual cortex could correspond to the degree of glaucoma-related visual field loss. Patients with glaucoma are often unaware of their visual field loss because that glaucoma-related visual sensitivity loss starts from periphery of visual field. Our results could be of clinical use for picking up asymptomatic glaucoma by detecting specific atrophy via brain MRI medical checkup.

  12. Prenatal oxytocin exposure influences emotional and social behaviors

    KASAHARA Yoshiyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2014/04 - 2017/03

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    Maternal stress during pregnancy increases the risk of psychiatric disorders of the offspring. In response to stress, the level of oxytocin is increased. While oxytocin is well known to play important roles in social interactions, including attachment between mother and child, as well as reproductive functions. In this study, we examined the effects of prenatal oxytocin exposure in mice to future emotional and social behaviors. Mice prenatally treated with oxytocin showed significantly increased anxiety behavior, and significantly lower social behaviors, compared with control animals. Interestingly, oxytocin gene expression level in the amygdala was significantly lower in mice prenatally treated with oxytocin. Our results indicated that prenatal oxytocin exposure influences anxiety and social behaviors after growing up, and therefore, increased level of oxytocin evoked by maternal stress needs to be aware as a risk indicator of deteriorating mental health of the offspring.

  13. Generation of biological models with AD / HD patients induced iPS cells

    SORA Ichiro, KASAHARA Yoshiyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    2012/04 - 2015/03

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    To generate Attention Deficit Hyperactivity Disorder (AD / HD) patients induced pluripotent stem cells (iPS cells), the selection of patients with appropriate diagnostic criteria are important. Diagnostic criteria of DSM-IV was used for AD / HD without comorbid disorders. Such as autism spectrum syndrome were carefully excluded for the subjects. We generated iPS cells from healthy individuals and AD / HD patients. Dopaminergic nervous system in the AD / HD which is considered to midbrain cortical limbic system that projects from the ventral tegmental area to the nucleus accumbens and frontal cortex plays an important role. We have studied on the differentiation to distinguish the midbrain limbic dopaminergic neurons and the nigrostriatal dopaminergic ones induced from the iPS cells.

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