研究者詳細

顔写真

ドウーデイ　カデイヤ
Daoudi Khadija
Daoudi Khadija
所属
大学院農学研究科　農芸化学専攻　生物化学講座（酵素化学分野）
職名
助教
学位

  • Ｐｈ．Ｄ．（Hassan II University of Casablanca）

  • Ｍ．Ｓ．（Hassan II University of Casablanca）

委員歴 1

  • Multidisciplinary Digital Publishing Institute (MDPI)　Volunteer Reviewer

    2026年3月 ～ 継続中

所属学協会 3

  • Asian Society of Arachnology

    2026年3月 ～ 継続中

  • International Society on Toxinology

    2026年1月 ～ 継続中

  • Laboratory of Enzymology, Graduate School of Agricultural Sciences, Tohoku University, Sendai 980-8572, Japan

    2023年5月 ～ 継続中

研究キーワード 1

  • Venomics, Ion channel-toxin interaction, drug discovery, biopesticides

研究分野 1

  • ライフサイエンス / 応用分子細胞生物学 /

受賞 1

  1. Second prize for poster

    2019年4月　The Polydisciplinary Faculty of Taroudant, Morocco　Evaluation of cytotoxic activity of Moroccan snake and scorpion venoms on cancer cell lines/ 2nd international scientific conference for research and ethics

論文 11

  1. Saccharomyces cerevisiae Models of Alzheimer's Disease to Screen Genes, Mutations, and Chemicals Affecting Amyloid Beta Production by γ-Secretase. 国際誌

    Eugene Futai, Mio Shiina, Ying Dai, Khadija Daoudi, Masafumi Hidaka, Tomohisa Ogawa

    Journal of visualized experiments : JoVE　(220)　2025年6月24日

    DOI： 10.3791/67733 　

    詳細を見る 詳細を閉じる

    γ-Secretase is a protease complex embedded in the cell membrane, consisting of the catalytic presenilin subunits (PS1 or PS2) and three additional co-factors: nicastrin, Aph-1, and Pen2. It cleaves the transmembrane domains of type-I transmembrane proteins, such as the amyloid precursor protein (APP) and Notch. The cleavage of APP generates the amyloid β peptides (Aβ), which accumulate in patients with Alzheimer's disease. Despite significant research, the exact mechanism of this unique proteolysis, which occurs within the lipid bilayers, is still not fully understood. To study the enzymatic properties of γ-secretase, we have established a yeast reporter system using artificial γ-secretase substrates containing APP or Notch fragments fused to the transcriptional activator Gal4. The γ-secretase activity was evaluated by transcriptional activation of reporter genes upon Gal4 release from the membrane-bound substrates, as assessed by the growth of yeast or β-galactosidase activity. Furthermore, we have developed an in vitro assay to identify the different forms of Aβ produced from yeast microsomes. These yeast models provide a platform to screen mutations, genes, and compounds that affect γ-secretase function. By studying the loss of function properties of PS1 familial Alzheimer's disease (FAD) mutants, it is possible to screen for FAD suppressor mutations and γ-secretase modulators (GSMs), in addition to γ-secretase inhibitors (GSIs). In this report, we describe the genetic and biochemical methods used to study γ-secretase activity in the yeast system with the essential steps of the protocol for the video.

  2. Microcystis viridis NIES-102 Cyanobacteria Lectin (MVL) Interacts with SARS-CoV-2 Spike Protein Receptor Binding Domains (RBDs) via Protein-Protein Interaction. 国際誌

    Zhengguang Wang, Zhihan Yang, Mami Shishido, Khadija Daoudi, Masafumi Hidaka, Hiroaki Tateno, Eugene Futai, Tomohisa Ogawa

    International journal of molecular sciences　25　(12)　2024年6月18日

    DOI： 10.3390/ijms25126696 　

    詳細を見る 詳細を閉じる

    The emergence of coronavirus disease 2019 (COVID-19) posed a major challenge to healthcare systems worldwide, especially as mutations in the culprit Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) complicated the development of vaccines and antiviral drugs. Therefore, the search for natural products with broad anti-SARS-CoV-2 capabilities is an important option for the prevention and treatment of similar infectious diseases. Lectins, which are widely recognized as antiviral agents, could contribute to the development of anti-SARS-CoV-2 drugs. This study evaluated the binding affinity of six lectins (including the cyanobacterial lectin from Microcystis viridis NIES-102 (MVL), and Jacalin, a lectin from the breadfruit, Artocarpus altilis) to the receptor binding domain (RBD) of the spike protein on the original (wild) SARS-CoV-2 and three of its mutants: Alpha, Delta, and Omicron. MVL and Jacalin showed distinct binding affinity to the RBDs of the four SARS-CoV-2 strains. The remaining four lectins (DB1, ConA, PHA-M and CSL3) showed no such binding affinity. Although the glycan specificities of MVL and Jacalin were different, they showed the same affinity for the spike protein RBDs of the four SARS-CoV-2 strains, in the order of effectiveness Alpha > Delta > original > Omicron. The verification of glycan-specific inhibition revealed that both lectins bind to RBDs by glycan-specific recognition, but, in addition, MVL binds to RBDs through protein-protein interactions.

  3. A Metalloproteinase Cocktail from the Venom of Protobothrops flavoviridis Cleaves Amyloid Beta Peptides at the α-Cleavage Site

    Eugene Futai, Hajime Kawasaki, Shinichi Sato, Khadija Daoudi, Masafumi Hidaka, Taisuke Tomita, Tomohisa Ogawa

    Toxins　15　(8)　2023年8月

    DOI： 10.3390/toxins15080500 　

    eISSN：2072-6651

  4. Anti-Cancer Activity of Buthus occitanus Venom on Hepatocellular Carcinoma in 3D Cell Culture

    Ayoub Lafnoune, Su Yeon Lee, Jin Yeong Heo, Khadija Daoudi, Bouchra Darkaoui, Salma Chakir, Rachida Cadi, Khadija Mounaji, David Shum, Haeng Ran Seo, Naoual Oukkache

    Molecules　27　(7)　2022年4月

    DOI： 10.3390/molecules27072219 　

    eISSN：1420-3049

  5. Induced pathophysiological alterations by the venoms of the most dangerous Moroccan scorpions Androctonus mauretanicus and Buthus occitanus: A comparative pathophysiological and toxic-symptoms study 国際誌

    Bouchra Darkaoui, Ayoub Lafnoune, Fatima Chgoury, Khadija Daoudi, Salma Chakir, Khadija Mounaji, Mehdi Karkouri, Rachida Cadi, Oukkache Naoual

    Human and Experimental Toxicology　41　9603271211072872-9603271211072872　2022年1月7日

    DOI： 10.1177/09603271211072872 　

    ISSN：0960-3271

    eISSN：1477-0903

  6. Mass spectrometry-based top-down and bottom-up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom

    Khadija Daoudi, Christian Malosse, Ayoub Lafnoune, Bouchra Darkaoui, Salma Chakir, Jean Marc Sabatier, Julia Chamot-Rooke, Rachida Cadi, Naoual Oukkache

    FEBS Open Bio　11　(7)　1867-1892　2021年7月

    DOI： 10.1002/2211-5463.13143 　

    eISSN：2211-5463

  7. Top-down and Bottom-up Approaches Revealed New Categories of Peptides from the Venom of Moroccan Scorpion Androctonus mauretanicus

    Venom and toxins　2021年4月

    DOI： 10.2174/2666121701666210401120929 　

  8. Anti-cancer effect of moroccan cobra naja haje venom and its fractions against hepatocellular carcinoma in 3d cell culture

    Ayoub Lafnoune, Su Yeon Lee, Jin Yeong Heo, Imane Gourja, Bouchra Darkaoui, Zaineb Abdelkafi-Koubaa, Fatima Chgoury, Khadija Daoudi, Salma Chakir, Rachida Cadi, Khadija Mounaji, Najet Srairi-Abid, Naziha Marrakchi, David Shum, Haeng Ran Seo, Naoual Oukkache

    Toxins　13　(6)　2021年

    DOI： 10.3390/toxins13060402 　

    eISSN：2072-6651

  9. Screening of active biomolecules from the venom of the Moroccan viper Daboia mauritanica

    Chakir Salma, Daoudi Khadija, Darkaoui Bouchra, Lafnoune Ayoub, Hmyne Aziz, Oukkache Naoual

    EC Pharmacol. EC Pharmacol (2019). Toxicol.7, 144-149.　7　144-149　2019年12月

  10. Development of an ELISA Assay for the Quantification of Venom Levels of Scorpions “Androctonus mauretanicus” and “Buthusoccitanus” in Stung Patients

    Khadiia Daoudi, Salma Chakir, Fatima Chgoury, ImaneGourja, Abdelaziz Hmyene, Rachida Cadi, Naoual Oukkache. EC Pharmacology, Toxicology

    EC Pharmacology and Toxicology 6.8 (2018): 679-688.　679-688　2018年

  11. Consequences of Androctonus mauretanicus and Buthus occitanus scorpion venoms on electrolyte levels in rabbits

    Khadija Daoudi, Fatima Chgoury, Myriam Rezzak, Oussama Bourouah, Lotfi Boussadda, Abdelaziz Soukri, Jean Marc Sabatier, Naoual Oukkache

    Heliyon　3　(1)　2017年1月1日

    DOI： 10.1016/j.heliyon.2016.e00221 　

    ISSN：2405-8440

︎全件表示 ︎最初の5件までを表示

MISC 3

  1. インテグリンの活性を特異的に制御するハブ毒由来ペプチドの開発

    佐藤拓郎, 二井勇人, 日高將文, DAOUDI Khadija, 小川智久

    日本生化学会大会(Web)　97th　2024年

  2. Effects of Androctonus mauretanicus and Buthus occitanus scorpion venoms on serum electrolytes in injected rabbits: A 24 hour time-course study

    Toxicon　2018年7月

    DOI： 10.1016/j.toxicon.2017.12.034 　

  3. Characterization of Am IT, an anti-insect β-toxin isolated from the venom of scorpionAndroctonus mauretanicus

    Khadija Daoudi, Myriam Rezzak, Oussama Bourouah, Fatima Chgoury, Naoual Oukkache

    Toxicon　2018年7月

    DOI： 10.1016/j.toxicon.2017.12.020 　

講演・口頭発表等 20

  1. What about the Japanese spider venoms from a transcriptomic view?

    Khadija DAOUDI, Zuohan WANG, Takuro SATO, Eugene FUTAI, Tomohisa OGAWA

    The 53rd meeting of the Japanese Society of Toxicology

  2. Transformer-Based Classification of Neurotoxic Ion Channel Peptides with Learned Embeddings, Class Rebalancing, and Focal Loss Optimization.

    Zuohan WANG, Khadija DAOUDI, Takuro SATO, Eugene FUTAI, Yiheng QUAN, Tomohisa OGAWA

    Gordon Conference Reference:Patterns and Processes in Venom Evolution: Comparative, Functional, and Translational Insights

  3. Unveiling the Molecular Diversity of Japanese Spider Venoms: A Transcriptomic View

    Khadija DAOUDI, Zuohan WANG, Takuro SATO, Eugene FUTAI, Tomohisa OGAWA

    Gordon Conference Reference:Patterns and Processes in Venom Evolution: Comparative, Functional, and Translational Insights

  4. Exploring the Molecular Diversity of Japanese Spider Venoms: A Transcriptomic View 招待有り

    DAOUDI Khadija, WANG Zuohan, SATO Takuro, HIDAKA Masafumi, FUTAI Eugene, OGAWA Tomohisa

    Asia-Africa Research Exchange Symposium

  5. 8th Venoms to Drugs 2023

    8th Venom to Drug Conference (V2D), Brisbane, Australia

  6. The 2nd International Electronic Conference on Toxins- virtual event

    (July 14–28, 2023).

  7. Anticancer potential of Androctonus mauritanicus scorpion neurotoxins: In silico modeling approach by virtual screening

    Khadija Daoudi, Samah Nassir, Naoual Oukkache

    9th International Toxinology Meeting Venoms and Toxins (Oxford) 2022

  8. Nouvelles neurotoxines isolées à partir du venin de scorpion marocain Androctonus mauritanicus (Buthidae)

    Khadija Daoudi, Philippe Chan-Tchi-Song, Marie-Laure Walet-Balieu, Laurent coque, Magalie dupré, David vaudry, Reto Stöcklin, Rachida Cadi, Naoual Oukkache

    Louis Pasteur Bicentenary, Mohammed VI Multidisciplinary University (UMP6), Benguerir, Morocco (May 30-31, 2022).

  9. Mass spectrometry-based proteomics approaches for qualitative analysis of the Moroccan Androctonus mauritanicus scorpion venom 招待有り

    Khadija Daoudi, Philippe Chan, Marie-Laure Walet-Balieu, Laurent coque, Julia chamot-Rooke, Rachida Cadi, David vaudry, Reto Stöcklin, Naoual Oukkache

    2nd International Webinar on Mass Spectrometry and Analytical Techniques.

  10. Exhaustive characterization of venoms from Moroccan scorpions through new proteomic approaches

    Daoudi Khadija, Lafnoune A, Chakir S,Cadi R, Oukkache N.

    25th Meeting of the French Society of Toxinology (SFET) “Toxins and health: from molecules to organisms -Institut Pasteur, Paris, France.

  11. Effects of Androcotonus mauretanicus and Buthus occitanus scorpion venoms on serum electrolytes in injected rabbits: a 24-hour time course study

    Daoudi K., Chgoury F., Oukkache N.

    23rd Meeting of the French Society of Toxinology (SFET)"Toxins: Immunity, Inflammation and Pain" - Institut Pasteur Paris – France.

  12. Moroccan scorpion, a great source of neurotoxins: a mass spectrometry-based proteomic view 招待有り

    Lecture at the Graduated School of Agricultural Science

  13. Proteomic approaches to characterize novel neurotoxins from A. mauritanicus scorpion venom

    Khadija Daoudi, Philippe Chan, Marie-Laure Walet-Balieu, Laurent coque, Magalie dupré, Rachida Cadi, David vaudry, Reto Stöcklin, Naoual Oukkache

    10th Austrian Peptide Symposium

  14. Novel neurotoxins isolated from the Moroccan Androctonus mauritanicus scorpion venom (Buthidae)

    Khadija Daoudi, Philippe Chan, Marie-Laure Walet-Balieu, Laurent coque, Magalie dupré, Rachida Cadi, David vaudry, Reto Stöcklin, Naoual Oukkache

    8 th International Toxinology Meeting Venoms and Toxins (Oxford) 2021　2021年8月25日

  15. Evaluation of cytotoxic activity of Moroccan snake and scorpion venoms on cancer cell lines

    Khadija Daoudi, Philippe Chan, David Vaudry, Naoual Oukkache

    2nd international scientific conference for research and ethics, The Polydisciplinary Faculty of Taroudant, Morocco　2019年4月19日

  16. Evaluation of the biological activity of Moroccan snake and scorpions venoms on granule neurons and cancer cell lines

    Khadija Daoudi, Magali Basille, Philippe Chan, Rachida Cadi, David Vaudry, Naoual Oukkache

    IRIB 3R Symposium : Day on the “Development of regenerative strategies for the repair of the nervous system and functional recovery” Rouen- France

  17. Exhaustive characterization of several Moroccan scorpion venoms

    Khadija Daoudi, Ayoub Lafnoune, Salma Chakir, Bouchra Darkaoui, Hafsa Bensrhir, Fatima Chgoury, Rachida Cadi, e, Naoual Oukkache

    22nd LARC-Neurosciences meeting in Rouen – France

  18. Caractérisation exhaustive des venins des scorpions marocains par les nouvelles approches protéomiques

    Khadija Daoudi, Ayoub Lafnoune, Salma Chakir, Bouchra Darkaoui, Hafsa Bensrhir, Fatima Chgoury, Rachida Cadi, e, Naoual Oukkache

    7th International Congress of Toxicology - Antipoison and Pharmacological Center of Morocco, Rabat Morocco　2018年10月10日

  19. Development of an ELISA assay for identification and quantification of the venom of two Moroccan scorpions

    Khadija Daoudi, Fatima Chgoury, Naoual Oukkache

    Pasteur Network Meeting - Institut Pasteur Paris-France.　2016年11月28日

  20. Mise au point d'un test ELISA pour l'identification et la quantification du venin de deux scorpions marocains

    Khadija Daoudi, Fatima Chgoury, Naoual Oukkache.

    Journée Scientifique de l’institut Pasteur du Maroc

︎全件表示 ︎最初の5件までを表示

学術貢献活動 1

  1. Invited peer reviewer for International Scientific Journal

    2023年8月16日 ～ 継続中

    学術貢献活動種別: 査読等