研究者詳細

顔写真

カマノ　ユウヤ
鎌野　優弥
Yuya Kamano
所属
病院　口腔修復系診療科　歯内療法科
職名
講師
学位

  • 博士（歯学）（大阪大学）

経歴 3

  • 2023年4月 ～ 継続中
    東北大学病院　病院（歯科診療部門） 歯内療法科　講師

  • 2020年4月 ～ 2023年3月
    厚生労働省東北厚生局　健康福祉部医事課　再生医療等推進専門官

  • 2015年4月 ～ 2020年3月
    東北大学病院　クラウンブリッジ補綴学　助教

学歴 2

  • 大阪大学大学院　歯学研究科

    2011年4月 ～ 2015年3月

  • 大阪大学　歯学部　歯学科

    2004年4月 ～ 2010年3月

研究キーワード 3

  • 歯科保存学

  • 骨代謝

  • 分子細胞生物学

研究分野 1

  • ライフサイエンス / 保存治療系歯学 /

受賞 2

  1. デンツプライ賞

    2015年　日本補綴歯科学会

  2. Arthur Frechette Award Finalist

    2012年　IADR

論文 22

  1. Biocompatibility of a Novel Light-Curable Hydrogel-Based Root Canal Obturation Material: In Vivo and In Vitro Analyses. 国際誌

    Moe Sandar Kyaw, Yoshio Yahata, Masato Nakano, Fusami Toyama, Chen Ke, Wang Shuai, Yuya Kamano, Futaba Harada, Tomose Noguchi, Masahiro Saito

    Journal of endodontics　51　(12)　1814-1822　2025年12月

    DOI： 10.1016/j.joen.2025.08.017 　

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    INTRODUCTION: This study aims to evaluate the biocompatibility of a new light-curable hydrogel-based root canal obturation material, OdneFill, through in vivo and in vitro analyses. METHODS: Intentional overinstrumentation and overobturation were performed on the mandibular first molars of 10-week-old male rats, divided into 4 groups: group 1 (instrumentation only), group 2 (OdneFill), group 3 (gutta-percha + AH Plus), and group 4 (sound teeth). Mandibles were dissected after 3, 28, and 90 days for micro-computed tomography, histological analysis, and immunohistochemical staining and analyzed by two-way analysis of variance and post hoc Tukey test. Cytotoxicity and proinflammatory cytokine expression were assessed using RAW 264.7 cells and analyzed by 1-way analysis of variance and Tukey test (α = 0.05). RESULTS: Micro-computed tomographic analysis showed no significant difference in bone resorption (P > .05). However, AH Plus exhibited a higher inflammatory score (score: 1%-90%, score: 2%-10%) with increased neutrophil and macrophage infiltration in immunostaining (P < .05) compared to the instrumentation only group at day 90. In contrast, Odnefill showed comparable results (score: 0%-50%, score: 1%-30%, score: 2%-20%) to the instrumentation-only group (P > .05). Moreover, Odnefill did not affect the viability of RAW 264.7 cells, whereas the AH Plus extract decreased cell viability and upregulated inflammatory cytokines such as interleukin-1 beta and interleukin-6. CONCLUSIONS: OdneFill demonstrated superior biocompatibility, and a minimal inflammatory response compared to AH Plus.

  2. Endodontic Regeneration Therapy: Current Strategies and Tissue Engineering Solutions. 国際誌

    Moe Sandar Kyaw, Yuya Kamano, Yoshio Yahata, Toshinori Tanaka, Nobuya Sato, Fusami Toyama, Tomose Noguchi, Marina Saito, Masato Nakano, Futaba Harada, Masahiro Saito

    Cells　14　(6)　2025年3月12日

    DOI： 10.3390/cells14060422 　

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    With increasing life expectancy and an aging population, the demand for dental treatments that preserve natural teeth has grown significantly. Among these treatments, endodontic therapies for pulpitis and apical periodontitis play a vital role, not only in keeping occlusal function, but also in preventing the exacerbation of systemic diseases. Both pulpitis and apical periodontitis are primarily caused by infections of the oral pathobiont within the root canal, leading to inflammation and destruction of the pulp, apical periodontal tissue, and bone. Standard root canal therapy aims to remove the infection source and facilitate natural tissue healing through the body's regenerative capacity. However, challenges remain, including limited tooth functionality after complete pulp removal in pulpitis and insufficient recovery of the large bone defect in apical periodontitis. To address these limitations, endodontic regenerative therapies have emerged as promising alternatives. Pulp regeneration therapy seeks to restore the functionality of dental pulp, while bone regeneration therapy aims to repair and regenerate large bone defects affected by apical periodontal tissue.

  3. Japanese Laws and the Current Status of Regenerative Medicine in the Tohoku Region. 国際誌

    Yuya Kamano, Noboru Terajima, Yuta Chiba, Venkata V Suresh, Masahiro Saito

    The journal of contemporary dental practice　24　(2)　120-128　2023年2月1日

    DOI： 10.5005/jp-journals-10024-3487 　

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    AIM: The aim of this study was to review Japanese laws regarding regenerative medicine and the current status of clinical application of regenerative medicine, to learn about the advantages and problems, and to thereby serve as a reference for measures necessary for the development of regenerative medicine. BACKGROUND: Regenerative medicine started in 1957 with the transplantation of hematopoietic stem cells, followed by the establishment of embryonic stem cells in 1981 and induced pluripotent stem cells in 2006, and continues to evolve progressively. At the same time, however, problems have emerged due to lax legal regulations, such as the use of treatments that lack scientific evidence. REVIEW RESULTS: The Japanese government enacted two laws to regulate regenerative medicine: the Law to Ensure the Safety of Regenerative Medicine and the Amend the Pharmaceutical Affairs Law in 2013. These laws were enacted with the aim of providing safe regenerative medicine promptly and smoothly and developing many regenerative medicine products. In these laws, regenerative medicine is defined as medical treatment that restores lost functions of damaged organs and tissues with the help of cellular and tissue-based products. Nowadays, there are two major methods of regenerative medicine. One representative method involves the transplantation of devices that activates self-regenerative ability by introducing living cells into patients' body. The other method is the activation and differentiation of endogenous stem cells with cell growth and differentiation factors. CONCLUSION: The current status of regenerative medicine in the Tohoku region after the enactment of these laws is described in detail. This clarified the advantages and disadvantages associated with regenerative medicine as it is currently practiced in Japan. CLINICAL SIGNIFICANCE: Development of regenerative medicine in dentistry will be advanced by learning about its clinical application in medicine.

  4. Rapid and efficient generation of cartilage pellets from mouse induced pluripotent stem cells by transcriptional activation of BMP-4 with shaking culture. 国際誌 査読有り

    Maolin Zhang, Kunimichi Niibe, Takeru Kondo, Phoonsuk Limraksasin, Hiroko Okawa, Xinchao Miao, Yuya Kamano, Masahiro Yamada, Xinquan Jiang, Hiroshi Egusa

    Journal of tissue engineering　13　(20417314221114616)　20417314221114616-20417314221114616　2022年7月

    DOI： 10.1177/20417314221114616 　

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    Induced pluripotent stem cells (iPSCs) offer an unlimited source for cartilage regeneration as they can generate a wide spectrum of cell types. Here, we established a tetracycline (tet) controlled bone morphogenetic protein-4 (BMP-4) expressing iPSC (iPSC-Tet/BMP-4) line in which transcriptional activation of BMP-4 was associated with enhanced chondrogenesis. Moreover, we developed an efficient and simple approach for directly guiding iPSC-Tet/BMP-4 differentiation into chondrocytes in scaffold-free cartilaginous pellets using a combination of transcriptional activation of BMP-4 and a 3D shaking suspension culture system. In chondrogenic induction medium, shaking culture alone significantly upregulated the chondrogenic markers Sox9, Col2a1, and Aggrecan in iPSCs-Tet/BMP-4 by day 21. Of note, transcriptional activation of BMP-4 by addition of tet (doxycycline) greatly enhanced the expression of these genes. The cartilaginous pellets derived from iPSCs-Tet/BMP-4 showed an oval morphology and white smooth appearance by day 21. After day 21, the cells presented a typical round morphology and the extracellular matrix was stained intensively with Safranin O, alcian blue, and type II collagen. In addition, the homogenous cartilaginous pellets derived from iPSCs-Tet/BMP-4 with 28 days of induction repaired joint osteochondral defects in immunosuppressed rats and integrated well with the adjacent host cartilage. The regenerated cartilage expressed the neomycin resistance gene, indicating that the newly formed cartilage was generated by the transplanted iPSCs-Tet/BMP-4. Thus, our culture system could be a useful tool for further investigation of the mechanism of BMP-4 in regulating iPSC differentiation toward the chondrogenic lineage, and should facilitate research in cartilage development, repair, and osteoarthritis.

  5. Titanium surface with nanospikes tunes macrophage polarization to produce inhibitory factors for osteoclastogenesis through nanotopographic cues. 国際誌

    Nadia Kartikasari, Masahiro Yamada, Jun Watanabe, Watcharaphol Tiskratok, Xindie He, Yuya Kamano, Hiroshi Egusa

    Acta biomaterialia　137　316-330　2022年1月1日

    出版者・発行元： Elsevier BV

    DOI： 10.1016/j.actbio.2021.10.019 　

    ISSN：1742-7061

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    Definitive prevention of inflammatory osteolysis around peri-implant bone tissue remains unestablished. M1 macrophages play a key role in the host defense against inflammatory osteolysis, and their polarization depends on cell shape. Macrophage polarization is controlled by environmental stimuli, particularly physicochemical cues and hence titanium nanosurface might tune macrophage polarization and function. This study determined whether titanium nanosurfaces with anisotropically patterned nanospikes regulates macrophage polarization for inhibiting osteoclast differentiation of osteoclast precursors. Alkaline-etching treatment with different protocols created two types of titanium nanosurfaces that had anisotropically patterned nanospikes with high or low distribution density, together with superhydrophilicity and the presence of hydroxyl groups. J774A.1 cells (mouse macrophage-like cell line), cultured on both titanium nanosurfaces, exhibited truly circulated shapes and highly expressed M1, but less M2, markers, without loss of viability. M1-like polarization of macrophages on both titanium nanosurfaces was independent of protein-mediated ligand stimulation or titanium surface hydrophilic or chemical status. In contrast, other smooth or micro-roughened titanium surfaces with little or no nanospikes did not activate macrophages under any culture conditions. Macrophage culture supernatants on both titanium nanosurfaces inhibited osteoclast differentiation of RAW264.7 cells (mouse osteoclast precursor cell line), even when co-incubated with osteoclast differentiation factors. The inhibitory effects on osteoclast differentiation tended to be higher in macrophages cultured on titanium nanosurfaces with denser nanospikes. These results showed that titanium nanosurfaces with anisotropically patterned nanospikes tune macrophage polarization for inhibiting osteoclast differentiation of osteoclast precursors, with nanotopographic cues rather than other physicochemical properties. STATEMENT OF SIGNIFICANCE: Peri-implant inflammatory osteolysis is one of the serious issues for dental and orthopedic implants. Macrophage polarization and function are key for prevention of peri-implant inflammatory osteolysis. Macrophage polarization can be regulated by the biomaterial's surface physicochemical properties such as hydrophilicity or topography. However, there was no titanium surface modification to prevent inflammatory osteolysis through immunomodulation. The present study showed for the first time that the titanium nanosurfaces with anisotropically patterned nanospikes, created by the simple alkali-etching treatment polarized macrophages into M1-like type producing the inhibitory factor on osteoclast differentiation. This phenomenon attributed to nanotopographic cues, but not hydrophilicity on the titanium nanosurfaces. This nanotechnology might pave the way to develop the smart implant surface preventing peri-implant inflammatory osteolysis through immunomodulation.

  6. Gene transfection achieved by utilizing antibacterial calcium phosphate nanoparticles for enhanced regenerative therapy. 国際誌

    Chen Xiang, Taichi Tenkumo, Toru Ogawa, Yoshiaki Kanda, Keisuke Nakamura, Midori Shirato, Viktoriya Sokolova, Matthias Epple, Yuya Kamano, Hiroshi Egusa, Keiichi Sasaki

    Acta biomaterialia　119　375-389　2021年1月1日

    DOI： 10.1016/j.actbio.2020.11.003 　

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    Protamine-coated multi-shell calcium phosphate (CaP) was developed as a non-viral vector for tissue regeneration therapy. CaP nanoparticles loaded with different amounts of plasmid DNA encoding bone morphogenetic protein 2 (BMP-2) and insulin-like growth factor 1 (IGF-1) were used to treat MC3T3E1 cells, and the yield of the released BMP-2 or IGF-1 was measured using ELISA 3 days later. Collagen scaffolds containing CaP nanoparticles were implanted into rat cranial bone defects, and BMP-2 and IGF-1 yields, bone formation, and bone mineral density enhancement were evaluated 28 days after gene transfer. The antibacterial effects of CaP nanoparticles against Streptococcus mutans and Aggregatibacter actinomycetemcomitans increased with an increase in the protamine dose, while they were lower for Staphylococcus aureus and Porphyromonas gingivalis. In the combination treatment with BMP-2 and IGF-1, the concentration ratio of BMP-2 and IGF-1 is an important factor affecting bone formation activity. The calcification activity and OCN mRNA of MC3T3E1 cells subjected to a BMP-2:IGF-1 concentration ratio of 1:4 was higher at 14 days. During gene transfection treatment, BMP-2 and IGF-1 were released simultaneously after gene transfer; the loaded dose of the plasmid DNA encoding IGF-1 did not impact the BMP-2 or IGF-1 yield or new bone formation ratio in vitro and in vivo. In conclusion, two growth factor-releasing systems were developed using an antibacterial gene transfer vector, and the relationship between the loaded plasmid DNA dose and resultant growth factor yield was determined in vitro and in vivo.

  7. Prolonged release of bone morphogenetic protein-2 in vivo by gene transfection with DNA-functionalized calcium phosphate nanoparticle-loaded collagen scaffolds. 国際誌 査読有り

    Taichi Tenkumo, Juan Ramón Vanegas Sáenz, Keisuke Nakamura, Yoshinaka Shimizu, Viktoriya Sokolova, Matthias Epple, Yuya Kamano, Hiroshi Egusa, Tsutomu Sugaya, Keiichi Sasaki

    Materials science & engineering. C, Materials for biological applications　92　172-183　2018年11月1日

    DOI： 10.1016/j.msec.2018.06.047 　

    ISSN：0928-4931

  8. Binding of PICK1 PDZ domain with calcineurin B regulates osteoclast differentiation 国際誌 査読有り

    Yuya Kamano, Jun Watanabe, Tsutomu Iida, Takeru Kondo, Hiroko Okawa, Hirofumi Yatani, Makio Saeki, Hiroshi Egusa

    Biochemical and Biophysical Research Communications　496　(1)　83-88　2018年1月29日

    DOI： 10.1016/j.bbrc.2017.12.173 　

    ISSN：1090-2104 0006-291X

  9. Amiloride-enhanced gene transfection of octa-arginine functionalized calcium phosphate nanoparticles. 国際誌 査読有り

    Juan Ramón Vanegas Sáenz, Taichi Tenkumo, Yuya Kamano, Hiroshi Egusa, Keiichi Sasaki

    PloS one　12　(11)　e0188347　2017年11月

    DOI： 10.1371/journal.pone.0188347 　

    ISSN：1932-6203

  10. アミロライドがオクタアルギニン/リン酸カルシウムを用いた遺伝子導入に及ぼす影響(Amiloride-enhanced gene transfection of octa-arginine functionalized calcium phosphate nanoparticles)

    バネガス・サネンズ・フアンラモン, 天雲 太一, 鎌野 優弥, 江草 宏, 小川 徹, 佐々木 啓一

    日本補綴歯科学会誌　9　(特別号)　276-276　2017年6月

    出版者・発行元： (公社)日本補綴歯科学会

    ISSN：1883-4426

    eISSN：1883-6860

  11. Gene delivery and expression systems in induced pluripotent stem cells 招待有り

    Zhang M, Niibe K, Kondo T, Kamano Y, Saeki M, Egusa H

    Interface Oral Health Science 2016, Springer　121-133　2017年

  12. Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells. 国際誌 査読有り

    Hiroko Okawa, Hiroki Kayashima, Jun-Ichi Sasaki, Jiro Miura, Yuya Kamano, Yukihiro Kosaka, Satoshi Imazato, Hirofumi Yatani, Takuya Matsumoto, Hiroshi Egusa

    Stem cells international　2016　6240794-6240794　2016年

    出版者・発行元： Hindawi Limited

    DOI： 10.1155/2016/6240794 　

    ISSN：1687-9678

  13. Gingival Fibroblasts as Autologous Feeders for Induced Pluripotent Stem Cells 査読有り

    G. Yu, H. Okawa, K. Okita, Y. Kamano, F. Wang, M. Saeki, H. Yatani, H. Egusa

    Journal of Dental Research　95　(1)　110-118　2016年1月1日

    出版者・発行元： SAGE Publications Inc.

    DOI： 10.1177/0022034515611602 　

    ISSN：1544-0591 0022-0345

  14. Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells 査読有り

    Hiroko Okawa, Hiroki Kayashima, Jun-Ichi Sasaki, Jiro Miura, Yuya Kamano, Yukihiro Kosaka, Satoshi Imazato, Hirofumi Yatani, Takuya Matsumoto, Hiroshi Egusa

    STEM CELLS INTERNATIONAL　2016年

    DOI： 10.1155/2016/6240794 　

    ISSN：1687-966X

    eISSN：1687-9678

  15. Controlled Osteogenic Differentiation of Mouse Mesenchymal Stem Cells by Tetracycline-Controlled Transcriptional Activation of Amelogenin. 国際誌 査読有り

    Fangfang Wang, Hiroko Okawa, Yuya Kamano, Kunimichi Niibe, Hiroki Kayashima, Thanaphum Osathanon, Prasit Pavasant, Makio Saeki, Hirofumi Yatani, Hiroshi Egusa

    PloS one　10　(12)　e0145677　2015年12月

    DOI： 10.1371/journal.pone.0145677 　

    ISSN：1932-6203

  16. PIH1D1 positively regulates mTOR complex 1-dependent rRNA transcription 査読有り

    Kamano Yuya, Saeki Makio, Kamisaki Yoshinori, Egusa Hiroshi

    JOURNAL OF PHARMACOLOGICAL SCIENCES　128　(3)　S120　2015年7月

    ISSN：1347-8613

  17. Feeder cell sources and feeder-free methods for human iPS cell culture 招待有り

    Yu G, Kamano Y, Wang F, Okawa H, Yatani H, Egusa H

    Interface Oral health Science 2014, Eds: Sasaki K, Suzuki O, Takahashi N, Springer　2014　145-159　2015年

    DOI： 10.1007/978-4-431-55192-8_12 　

  18. 歯肉を用いた医療応用に安全なiPS 細胞の開発 ―新たな再生歯科医療技術の創成に向けて― 招待有り

    江草 宏, 于 冠男, 鎌野優弥, 大川博子, 山本治毅, 矢谷博文

    日歯医学会誌　33　54-58　2014年3月

  19. PICK1 Regulates Osteoclastogenesis by Binding to Calcineurin B 査読有り

    Yuya Kamano, Makio Saeki, Hiroshi Egusa, Hirofumi Yatani, Yoshinori Kamisaki

    JOURNAL OF PHARMACOLOGICAL SCIENCES　124　93P-93P　2014年

    ISSN：1347-8613

    eISSN：1347-8648

  20. PIH1D1 interacts with mTOR complex 1 and enhances ribosome RNA transcription. 国際誌 査読有り

    Yuya Kamano, Makio Saeki, Hiroshi Egusa, Yoshito Kakihara, Walid A Houry, Hirofumi Yatani, Yoshinori Kamisaki

    FEBS letters　587　(20)　3303-8　2013年10月11日

    DOI： 10.1016/j.febslet.2013.09.001 　

    ISSN：0014-5793 1873-3468

  21. Exosome-bound WD repeat protein Monad inhibits breast cancer cell invasion by degrading amphiregulin mRNA. 国際誌 査読有り

    Makio Saeki, Hiroshi Egusa, Yuya Kamano, Yoshito Kakihara, Walid A Houry, Hirofumi Yatani, Shinzaburo Noguchi, Yoshinori Kamisaki

    PloS one　8　(7)　e67326　2013年7月

    DOI： 10.1371/journal.pone.0067326 　

    ISSN：1932-6203

  22. RPAP3 splicing variant isoform 1 interacts with PIH1D1 to compose R2TP complex for cell survival. 国際誌 査読有り

    Miki Yoshida, Makio Saeki, Hiroshi Egusa, Yasuyuki Irie, Yuya Kamano, Shinya Uraguchi, Maki Sotozono, Hitoshi Niwa, Yoshinori Kamisaki

    Biochemical and biophysical research communications　430　(1)　320-4　2013年1月4日

    DOI： 10.1016/j.bbrc.2012.11.017 　

    ISSN：0006-291X

    eISSN：1090-2104

︎全件表示 ︎最初の5件までを表示

MISC 1

  1. mTOR結合タンパク質PIH1D1がrRNA転写に及ぼす影響

    外園真規, 佐伯万騎男, 鎌野優弥, 鎌野優弥, 江草宏, 上崎善規

    日本薬理学会近畿部会プログラム・要旨集　122nd　2012年

共同研究・競争的資金等の研究課題 6

  1. 顎骨由来骨格幹細胞の造骨能力を担保する規格設定技術の開発

    齋藤 正寛, 李 銀星, 松田 安昌, 大庭 伸介, 石垣 司, 八幡 祥生, 鎌野 優弥, MOESANDARKYAW

    提供機関：Japan Society for the Promotion of Science

    制度名：Grants-in-Aid for Scientific Research

    研究種目：Grant-in-Aid for Scientific Research (B)

    研究機関：Tohoku University

    2025年4月1日 ～ 2028年3月31日

  2. 骨格幹細胞による軟骨内骨化を用いた新規骨再生技術の開発

    鎌野 優弥, 八幡 祥生, 齋藤 正寛, 中野 将人

    提供機関：Japan Society for the Promotion of Science

    制度名：Grants-in-Aid for Scientific Research

    研究種目：Grant-in-Aid for Scientific Research (C)

    研究機関：Tohoku University

    2024年4月1日 ～ 2027年3月31日

  3. iPS細胞の初期化機構の個体差解明を介した新規再生歯科補綴治療の探索

    鎌野 優弥

    2019年4月1日 ～ 2020年3月31日

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    使用する細胞の効率的な浮遊培養法の確立を行った。接着培養と比較して，浮遊培養では一度に大量の細胞を培養でき，遺伝子導入の際の試薬の量を節減し，費用や時間を節約することが可能となる。遺伝子導入を行う際のコストも浮遊培養のほうが圧倒的に安価となり，その意義は大きい。まずタイテック社製の振盪培養器を用いて，温度，振動数，二酸化炭素濃度等の条件を検討した。その結果，37度・120rpm・8%二酸化炭素濃度で使用する細胞が最も増殖することが明らかとなった。 次に使用するベクターの構築を行った。エントリーベクターをNBRC(NITE)より購入し，LRクローニングによりデスティネーションベクターに導入した。デスティネーションベクターの選考基準として発現効率・タグの付与・非ウイルス性等を考慮した。結果NativePure pcDNA Gateway Vectorが目的に適しており，これを選択した。このベクターはそれぞれC末端およびN末端にV5タグが付与されているものがあり，V5タグの位置やエントリーベクターの組み合わせによりタグを付与しないベクターを作製できることもメリットである。 浮遊培養環境下で作製したベクターを導入し，目的のタンパク質が発現していることが確認された。今後タンパク質の構造解析等を行う予定であったが，申請者の異動に伴い１年目で研究が廃止となった。今後，資格が回復した際には改めて申請行い継続していく所存である。

  4. iPS細胞のNotchシグナル制御を基盤とした顎骨再生の試み 競争的資金

    鎌野 優弥

    提供機関：Japan Society for the Promotion of Science

    制度名：Grants-in-Aid for Scientific Research

    研究種目：Grant-in-Aid for Young Scientists (B)

    研究機関：Tohoku University

    2017年4月1日 ～ 2019年3月31日

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    神経堤細胞分化に重要であるNotchシグナルをドキシサイクリン濃度依存的に調節することが可能である、NICD1-miPS細胞の樹立に成功した。これにより、iPS細胞の骨芽細胞分化におけるNotchシグナルの役割が検討できるようになった。 また、樹立した細胞株を用いて骨芽細胞分化を行った結果、Notchシグナルを恒常的に発現させた群では骨芽細胞分化がわずかながら抑制された。今後さらなる検討を行い、抑制機構の解明を行う予定である。

  5. iPS細胞の軟骨内骨化を利用した顎骨再生技術の開発

    江草 宏, 鎌野 優弥, 齋藤 正寛, 福本 敏, 大川 博子, 河阪 幸宏

    提供機関：Japan Society for the Promotion of Science

    制度名：Grants-in-Aid for Scientific Research

    研究種目：Grant-in-Aid for Challenging Exploratory Research

    研究機関：Tohoku University

    2016年4月1日 ～ 2018年3月31日

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    人工多能性幹細胞（iPS細胞）を用いた顎骨再生医療の開発は、より審美・機能的な補綴歯科治療を可能にするものと期待されている。本研究では、広範囲骨再生部位における軟骨内骨化の利点に着目し、試験管内でiPS細胞から骨/軟骨様細胞塊を作製する技術の開発を目的とした。本研究により、軟骨細胞および骨芽細胞分化誘導因子を併用することで、マウスiPS細胞の胚様体から、軟骨細胞/骨芽細胞の分化程度の割合を制御しながらハイブリッド軟骨/骨様細胞塊が作製可能であることを明らかにした。

  6. グルタミン酸輸送タンパク質PICK1を標的とした顎骨吸収抑制技術の開発 競争的資金

    鎌野 優弥, 江草 宏, 佐伯 万騎男

    提供機関：Japan Society for the Promotion of Science

    制度名：Grants-in-Aid for Scientific Research

    研究種目：Grant-in-Aid for Research Activity Start-up

    研究機関：Tohoku University

    2015年8月28日 ～ 2017年3月31日

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    重度の骨吸収に対して，骨代謝を調節する因子の探索に基づく新たな治療法の開発は重要な課題である。本研究では，神経伝達物質であるグルタミン酸が骨代謝を調節する作用を有することをに着目し，AMPAグルタミン酸レセプターと結合するPICK1をターゲットとして研究を行った。 PICK1の高発現は破骨細胞前駆細胞の破骨細胞分化を有意に促進し，PICK1のPDZドメイン結合阻害薬は破骨細胞分化を有意に抑制した。一方で，PICK1の高発現およびは骨芽細胞前駆細胞の骨芽細胞分化に影響を与えなかった。 以上のことから，PICK1は骨芽細胞分化に影響せず，破骨細胞分化を調整できることが示唆される。

︎全件表示 ︎最初の5件までを表示