研究者詳細

顔写真

ミヤザキ　ヒロフミ
宮崎　啓史
Hirofumi Miyazaki
所属
大学院医学系研究科　医科学専攻　細胞生物学講座（器官解剖学分野）
職名
講師
学位

  • 博士（医学）（東北大学）

  • 修士（保健学）（山口大学）

経歴 1

  • 2017年4月 ～ 継続中
    東北大学　大学院医学系研究科器官解剖学分野　助教

学歴 3

  • 東北大学　大学院医学系研究科医科学専攻

    2013年4月 ～ 2017年3月

  • 山口大学　大学院医学系研究科保健学専攻

    2011年4月 ～ 2013年3月

  • 山口大学　医学部保健学科

    2007年4月 ～ 2011年3月

研究分野 1

  • ライフサイエンス / 解剖学 /

論文 12

  1. FABP7 Protects Astrocytes Against ROS Toxicity via Lipid Droplet Formation. 国際誌 査読有り

    Ariful Islam, Yoshiteru Kagawa, Hirofumi Miyazaki, Subrata Kumar Shil, Banlanjo A Umaru, Yuki Yasumoto, Yui Yamamoto, Yuji Owada

    Molecular neurobiology　56　(8)　5763-5779　2019年8月

    DOI： 10.1007/s12035-019-1489-2 　

    ISSN：0893-7648

    詳細を見る 詳細を閉じる

    Fatty acid-binding proteins (FABPs) bind and internalize long-chain fatty acids, controlling lipid dynamics. Recent studies have proposed the involvement of FABPs, particularly FABP7, in lipid droplet (LD) formation in glioma, but the physiological significance of LDs is poorly understood. In this study, we sought to examine the role of FABP7 in primary mouse astrocytes, focusing on its protective effect against reactive oxygen species (ROS) stress. In FABP7 knockout (KO) astrocytes, ROS induction significantly decreased LD accumulation, elevated ROS toxicity, and impaired thioredoxin (TRX) but not peroxiredoxin 1 (PRX1) signalling compared to ROS induction in wild-type astrocytes. Consequently, activation of apoptosis signalling molecules, including p38 mitogen-activated protein kinase (MAPK) and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), and increased expression of cleaved caspase 3 were observed in FABP7 KO astrocytes under ROS stress. N-acetyl L-cysteine (NAC) application successfully rescued the ROS toxicity in FABP7 KO astrocytes. Furthermore, FABP7 overexpression in U87 human glioma cell line revealed higher LD accumulation and higher antioxidant defence enzyme (TRX, TRX reductase 1 [TRXRD1]) expression than mock transfection and protected against apoptosis signalling (p38 MAPK, SAPK/JNK and cleaved caspase 3) activation. Taken together, these data suggest that FABP7 protects astrocytes from ROS toxicity through LD formation, providing new insights linking FABP7, lipid homeostasis, and neuropsychiatric/neurodegenerative disorders, including Alzheimer's disease and schizophrenia.

  2. The role of fatty acid binding protein 7 in spinal cord astrocytes in a mouse model of experimental autoimmune encephalomyelitis. 国際誌 査読有り

    Kenyu Kamizato, Sho Sato, Subrata Kumar Shil, Banlanjo A Umaru, Yoshiteru Kagawa, Yui Yamamoto, Masaki Ogata, Yuki Yasumoto, Yuko Okuyama, Naoto Ishii, Yuji Owada, Hirofumi Miyazaki

    Neuroscience　409　120-129　2019年6月15日

    DOI： 10.1016/j.neuroscience.2019.03.050 　

    ISSN：0306-4522

    詳細を見る 詳細を閉じる

    Fatty acid binding protein 7 (FABP7) is expressed in astrocytes of the developing and mature central nervous system, and modulates astrocyte function by controlling intracellular fatty acid homeostasis. Astrocytes in the spinal cord have an important role in the process of myelin degeneration and regeneration. In the present study, the authors examined the role of FABP7 in astrocytes in a mouse model of experimental autoimmune encephalomyelitis (EAE), which is an established model of multiple sclerosis (MS). FABP7 was expressed in the white matter astrocytes and increased after EAE onset; particularly strong expression was observed in demyelinating regions. In FABP7-knockout (KO) mice, the onset of EAE symptoms occurred earlier than in wild type (WT) mice, and mRNA expression levels of inflammatory cytokines (IL-17 and TNF-α) were higher in FABP7-KO lumbar spinal cord than in WT lumbar spinal cord at early stage of EAE. Interestingly, however, the clinical score was significantly reduced in FABP7-KO mice compared with WT mice in the late phase of EAE. Moreover, the area exhibiting expression of fibronectin, which is an extracellular matrix protein mainly produced by astrocytes and inhibits remyelination of oligodendrocytes, was significantly decreased in FABP7-KO compared with WT mice. Collectively, FABP7 in astrocyte may have a role to protect from the induction of inflammation leading to demyelination in CNS at early phase of EAE. Moreover, FABP7 may be involved in the regulation of fibronectin production through the modification of astrocyte activation at late phase of EAE.

  3. Role of FABP7 in tumor cell signaling. 国際誌 査読有り

    Kagawa Y, Umaru BA, Ariful I, Shil SK, Miyazaki H, Yamamoto Y, Ogata M, Owada Y

    Advances in biological regulation　71　206-218　2019年1月

    DOI： 10.1016/j.jbior.2018.09.006 　

    ISSN：2212-4926

  4. FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase67 Promoter Region. 国際誌 査読有り

    Yui Yamamoto, Hiroyuki Kida, Yoshiteru Kagawa, Yuki Yasumoto, Hirofumi Miyazaki, Ariful Islam, Masaki Ogata, Yuchio Yanagawa, Dai Mitsushima, Kohji Fukunaga, Yuji Owada

    The Journal of neuroscience : the official journal of the Society for Neuroscience　38　(49)　10411-10423　2018年12月5日

    DOI： 10.1523/JNEUROSCI.1285-18.2018 　

    ISSN：0270-6474

  5. Glial Fatty Acid-Binding Protein 7 (FABP7) Regulates Neuronal Leptin Sensitivity in the Hypothalamic Arcuate Nucleus. 国際誌 査読有り

    Yuki Yasumoto, Hirofumi Miyazaki, Masaki Ogata, Yoshiteru Kagawa, Yui Yamamoto, Ariful Islam, Tetsuya Yamada, Hideki Katagiri, Yuji Owada

    Molecular neurobiology　55　(12)　9016-9028　2018年12月

    DOI： 10.1007/s12035-018-1033-9 　

    ISSN：0893-7648

  6. Astrocyte-Expressed FABP7 Regulates Dendritic Morphology and Excitatory Synaptic Function of Cortical Neurons 査読有り

    Majid Ebrahimi, Yui Yamamoto, Kazem Sharifi, Hiroyuki Kida, Yoshiteru Kagawa, Yuki Yasumoto, Ariful Islam, Hirofumi Miyazaki, Chie Shimamoto, Motoko Maekawa, Dai Mitsushima, Takeo Yoshikawa, Yuji Owada

    GLIA　64　(1)　48-62　2016年1月

    DOI： 10.1002/glia.22902 　

    ISSN：0894-1491

    eISSN：1098-1136

  7. Inhibition of Fatty Acid Synthase Decreases Expression of Stemness Markers in Glioma Stem Cells 査読有り

    Yuki Yasumoto, Hirofumi Miyazaki, Linda Koshy Vaidyan, Yoshiteru Kagawa, Majid Ebrahimi, Yui Yamamoto, Masaki Ogata, Yu Katsuyama, Hirokazu Sadahiro, Michiyasu Suzuki, Yuji Owada

    PLOS ONE　11　(1)　e0147717　2016年1月

    DOI： 10.1371/journal.pone.0147717 　

    ISSN：1932-6203

  8. Fatty Acid-Binding Protein 7 Regulates Function of Caveolae in Astrocytes through Expression of Caveolin-1 査読有り

    Yoshiteru Kagawa, Yuki Yasumoto, Kazem Sharifi, Majid Ebrahimi, Ariful Islam, Hirofumi Miyazaki, Yui Yamamoto, Tomoo Sawada, Hiroko Kishi, Sei Kobayashi, Motoko Maekawa, Takeo Yoshikawa, Eiichi Takaki, Akira Nakai, Hiroshi Kogo, Toyoshi Fujimoto, Yuji Owada

    GLIA　63　(5)　780-794　2015年5月

    DOI： 10.1002/glia.22784 　

    ISSN：0894-1491

    eISSN：1098-1136

  9. Fatty Acid Binding Protein 3 Is Involved in n-3 and n-6 PUFA Transport in Mouse Trophoblasts 査読有り

    Ariful Islam, Yoshiteru Kagawa, Kazem Sharifi, Majid Ebrahimi, Hirofumi Miyazaki, Yuki Yasumoto, Saki Kawamura, Yui Yamamoto, Syuiti Sakaguti, Tomoo Sawada, Nobuko Tokuda, Norihiro Sugino, Ryoji Suzuki, Yuji Owada

    JOURNAL OF NUTRITION　144　(10)　1509-1516　2014年10月

    DOI： 10.3945/jn.114.197202 　

    ISSN：0022-3166

    eISSN：1541-6100

  10. Fatty Acid Binding Protein 7 Regulates Phagocytosis and Cytokine Production in Kupffer Cells during Liver Injury 査読有り

    Hirofumi Miyazaki, Tomoo Sawada, Miwa Kiyohira, Zhiqian Yu, Keiji Nakamura, Yuki Yasumoto, Yoshiteru Kagawa, Majid Ebrahimi, Ariful Islam, Kazem Sharifi, Saki Kawamura, Takanori Kodama, Yui Yamamoto, Yasuhiro Adachi, Nobuko Tokuda, Shuji Terai, Isao Sakaida, Toshizo Ishikawa, Yuji Owada

    AMERICAN JOURNAL OF PATHOLOGY　184　(9)　2505-2515　2014年9月

    DOI： 10.1016/j.ajpath.2014.05.015 　

    ISSN：0002-9440

    eISSN：1525-2191

  11. Differential expression and regulatory roles of FABP5 and FABP7 in oligodendrocyte lineage cells 査読有り

    Kazem Sharifi, Majid Ebrahimi, Yoshiteru Kagawa, Ariful Islam, Tuerhong Tuerxun, Yuki Yasumoto, Tomonori Hara, Yui Yamamoto, Hirofumi Miyazaki, Nobuko Tokuda, Takeo Yoshikawa, Yuji Owada

    CELL AND TISSUE RESEARCH　354　(3)　683-695　2013年12月

    DOI： 10.1007/s00441-013-1730-7 　

    ISSN：0302-766X

    eISSN：1432-0878

  12. Activation of Different Signals Identified with Glia Cells Contribute to the Progression of Hyperalgesia 査読有り

    Satoru Yamamoto, Yusuke Kishishita, Mitsuhiro Yoshida, Daisuke Miura, Hidenori Suzuki, Kozo Ishikawa, Hirofumi Miyazaki, Junzo Nojima, Misa Yamamoto, Toshizo Ishikawa

    CELLULAR AND MOLECULAR NEUROBIOLOGY　33　(2)　167-174　2013年3月

    DOI： 10.1007/s10571-012-9881-8 　

    ISSN：0272-4340

    eISSN：1573-6830

︎全件表示 ︎最初の5件までを表示

講演・口頭発表等 5

  1. 脱髄疾患におけるアストロサイトの脂肪酸結合蛋白質FABP7の機能的役割の検討

    宮崎啓史, 神里賢勇, 佐藤匠, 香川慶輝, 大和田祐二

    第124回日本解剖学会総会・全国学術集会　2019年

  2. マクロファージの細胞内代謝変化による抗炎症性機能活性化とFABP7の関与の検討

    宮崎啓史, 宍戸愛, 安本有希, 香川慶輝, 大和田祐二

    第123回日本解剖学会総会・全国学術集会　2018年

  3. FABPによって制御される細胞内代謝がマクロファージ機能に及ぼす影響

    宮崎啓史, 大和田祐二

    第122回日本解剖学会総会・全国学術集会　2017年

  4. 高脂肪食摂取肥満マウスにおける肝マクロファージのFABP7の機能的役割の検討

    第121回日本解剖学会総会・全国学術集会　2016年

  5. 高脂肪食誘導脂肪性肝疾患モデルにおけるKupffer細胞のFABP7の機能的役割の検討

    第120回日本解剖学会総会・全国学術集会　2015年