Details of the Researcher

PHOTO

Takakazu Nakabayashi
Section
Graduate School of Pharmaceutical Sciences
Job title
Professor
Degree

  • Doctor of Science (The University of Tokyo)

Research History 7

  • 2014/05 - Present
    Tohoku University　Graduate School of Pharmaceutical Sciences

  • 2002/06 - 2014/04
    Hokkaido University　Research Institute for Electronic Science

  • 1997/12 - 2002/05
    Institute for Molecular Science

  • 1996/04 - 1997/11
    日本学術振興会　特別研究員 (DC2)

  • 1995/04 - 1997/11
    東京大学院理学系研究科　化学専攻　博士課程中退

  • 1993/04 - 1995/03
    東京大学大学院理学研究科　化学専攻　修士課程

  • 1989/04 - 1993/03
    The University of Tokyo　Faculty of Science, Department of Chemistry

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Education 3

  • The University of Tokyo

    - 1997

  • The University of Tokyo

    - 1995

  • The University of Tokyo　Faculty of Science　Department of Chemistry

    - 1993

Professional Memberships 7

  • 日本蛋白質科学会

  • 日本生物物理学会

  • THE PHARMACEUTICAL SOCIETY OF JAPAN

  • 溶液化学研究会

  • 日本分光学会

  • 分子科学会

  • 日本化学会

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Research Interests 17

  • Aggregation of proteins

  • Supersulfides

  • Neurodegenerative disease

  • Intracellular crowding environment

  • Liquid-liquid phase separation

  • Autofluorescence lifetime

  • Fluorescence lifetime

  • Molecular science

  • Raman imaging

  • Physical Pharmaceutical Science

  • 時間分解分光

  • 生物物理化学

  • Brillouin imaging

  • Vibrational Spectroscopy

  • Cell

  • Protein

  • Molecular Spectroscopy

Research Areas 6

  • Life sciences / Pharmaceuticals - analytical and physicochemistry /

  • Natural sciences / Bio-, chemical, and soft-matter physics /

  • Nanotechnology/Materials / Nanobioscience /

  • Nanotechnology/Materials / Analytical chemistry /

  • Nanotechnology/Materials / Biochemistry /

  • Nanotechnology/Materials / Basic physical chemistry /

Awards 4

  1. 第41回日本化学会学術賞

    2024　日本化学会

  2. 第14回日本光医学・光生物学会賞

    2023　日本光医学・光生物学協会

  3. 平成20年度日本化学会北海道支部奨励賞

    2009　日本化学会北海道支部

  4. 分子科学研究奨励森野基金

    2007　森野基金

Papers 131

  1. Size of Biomolecular Condensates Dictates Fate in Liquid–Solid Phase Transitions through Amorphous–Amyloid Competition Peer-reviewed

    Junka Kawakami, Taiki Ozawa, Yoko Maruyama, Honoka Ishikawa, Yuto Oshita, Kota Yamauchi, Koichi Kobayashi, Shinji Kajimoto, Takakazu Nakabayashi, Shunsuke Tomita, Tanushree Agarwal, Tomas Sneideris, Kichitaro Nakajima, Kentaro Shiraki, Hideki Taguchi, Akihide Hibara, Tuomas Knowles, Eri Chatani, Yuta Mizuno, Yumiko Ohhashi, Mao Fukuyama

    Journal of the American Chemical Society　2026/06/06

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/jacs.6c02816 　

    ISSN： 0002-7863

    eISSN： 1520-5126

  2. Label-free Raman chemometric analysis for in situ authentication and molecular profiling of Wagyu beef under retail packaging. International-journal Peer-reviewed

    Jia-En Kao, Chang-Chun Li, Ming-Syue Lin, Kohei Yokosawa, Takakazu Nakabayashi, Tai-Sheng Yeh, Ian Liau

    Talanta　303　129460-129460　2026/06/01

    DOI： 10.1016/j.talanta.2026.129460 　

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    Wagyu beef's distinctive flavor and tenderness arise from its high levels of unsaturated intramuscular triglycerides. Although these compositional distinctions provide a unique Raman signature, extensive band overlap and background attenuation from packaging and frozen conditions hinder reliable in situ classification and constrain the interpretability of raw spectra. This study presents a label-free Raman spectroscopic and chemometric approach for authenticating Wagyu beef under realistic retail-like conditions. A supervised partial least squares discriminant analysis (PLS-DA) model was developed using spectra from unwrapped adipose tissue and evaluated with an independent validation set of frozen, plastic-wrapped samples from multiple breeds and suppliers. The model achieved 100% sample-level classification accuracy, supported by receiver operating characteristic and confusion-matrix analyses. To elucidate the molecular basis of discrimination and resolve spectral congestion, a two-stage decomposition combining singular value decomposition (SVD) and nonnegative matrix factorization (NMF) was applied, followed by nonnegative least squares (NNLS) fitting with pure triglyceride standards. The analyses yielded chemically interpretable components, revealing enrichment of unsaturated triglycerides in Wagyu beef consistent with established compositional data. To our knowledge, this is the first report applying Raman-based PLS-DA for Wagyu authentication and integrating SVD, NMF, and NNLS for molecular-level interpretation in food authentication. Overall, Raman spectroscopy integrated with interpretable spectral decomposition provides a rapid, nondestructive, and chemically transparent tool for meat authentication and quality assurance.

  3. Lipids Contribute to Heterochromatin Condensation Revealed by Quantitative Raman–Brillouin Microscopy Peer-reviewed

    Masato Machida, Shinji Kajimoto, Ren Shibuya, Mayu Isono, Mai Watabe, Yukako Oma, Kayo Hibino, Kentaro Fujii, Masaki Okumura, Masahiko Harata, Atsushi Shibata, Takakazu Nakabayashi

    JACS Au　2026/04/24

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/jacsau.6c00082 　

    ISSN： 2691-3704

    eISSN： 2691-3704

  4. Elucidation of neuronal differentiation induced by nanosecond pulsed electric fields using Raman microscopy Peer-reviewed

    Ohsuke Irikura, Shinji Kajimoto, Kazunori Ban, Takakazu Nakabayashi

    Sensors and Actuators B: Chemical　140051-140051　2026/04

    Publisher: Elsevier BV

    DOI： 10.1016/j.snb.2026.140051 　

    ISSN： 0925-4005

  5. Liquid-liquid phase separation-assisted Raman microscopy for sensitive and label-free analysis of enzymatic reactions and protein-small molecule interactions. International-journal Peer-reviewed

    Lisa Kageyama, Shinya Tahara, Reona Tobita, Shinji Kajimoto, Takakazu Nakabayashi

    The Analyst　151　(3)　807-815　2026/02/02

    DOI： 10.1039/d5an00973a 　

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    Raman spectroscopy enables label-free and non-destructive structural analysis of biomolecules; however, its application is limited by the inherently weak Raman signals, which necessitate high concentrations of biomolecules for detection. In our previous study, we developed a liquid-liquid phase separation (LLPS)-assisted Raman method, in which biomacromolecules are concentrated into PEG-induced droplets, enabling the acquisition of high signal-to-noise (S/N) Raman spectra from dilute solutions with small volumes, such as 30 μM and 50 μL. We demonstrate here its broad analytical utility for several applications, including real-time monitoring of catalytic reactions such as RNA degradation and quantitative detection of protein-small molecule interactions exemplified by the avidin-biotin system. Furthermore, small molecules such as amino acids, monosaccharides and supersulfides were successfully concentrated, allowing their Raman spectra to be obtained with markedly improved S/N ratios. This technique thus provides a simple, highly sensitive and versatile analytical platform for Raman-based biochemical studies, with wide potential applications in analyzing biomolecular structures and intermolecular interactions, as well as diagnostics.

  6. Molecular Mechanism of Metal-Ion-Induced Protofibril Formation of Ataxin-3 Peer-reviewed

    Ippei Suzuki, Shinya Tahara, Nozomi Goso, Takahiro Morito, Dai Kato, Yusuke Hatakawa, Seon Hwa Lee, Tomoyuki Oe, Kunisato Kuroi, Takakazu Nakabayashi

    Bulletin of the Chemical Society of Japan　99　uoag009　2026/01/22

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/bulcsj/uoag009 　

    ISSN： 0009-2673

    eISSN： 1348-0634

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    Abstract Ataxin-3, the causative protein of Machado-Joseph disease, comprises an enzymatic domain, Josephin domain (JD), and a polyglutamine repeat (polyQ). The polyQ expansion promotes the fibrillogenesis of ataxin-3, which is responsible for the disease onset. The fibrillogenesis proceeds through two distinct steps: protofibril formation, in which JD undergoes self-assembly, and subsequent fibrillation. Both polyQ elongation and metal ions accelerate fibrillation; however, the effects of these factors on each stage remain unclear. This study comprehensively analyzed the effects of metal ions on ataxin-3 protofibril formation using spectroscopic analyses. The protofibril formation of JD and a 28-residue polyQ tract (Q28) did not complete within 14 days under metal-free conditions. In contrast, the introduction of Cu²⁺ or Zn²⁺ markedly accelerated the process: JD denatured within one day, and protofibrils formed within four days. The acceleration was similar between JD and Q28, indicating that metal binding primarily affects JD. SH assays and mass spectrometry showed that Cu²⁺ binds to thiols and induces intra- and intermolecular disulfide bonds, whereas Zn²⁺ binds to thiols but does not promote disulfide formation. These observations suggest that Cu²⁺ and Zn²⁺ enhance the protofibril formation through interactions with specific JD sites, but the underlying mechanisms differ between the two metal ions.

  7. Ca2+-driven PDIA6 biomolecular condensation ensures proinsulin folding Peer-reviewed

    Young-Ho Lee, Tomohide Saio, Mai Watabe, Motonori Matsusaki, Shingo Kanemura, Yuxi Lin, Taro Mannen, Tsubura Kuramochi, Yuka Kamada, Katsuya Iuchi, Michiko Tajiri, Kotono Suzuki, Yan Li, Yunseok Heo, Kotone Ishii, Kenta Arai, Kazunori Ban, Mayuko Hashimoto, Shuichiro Oshita, Satoshi Ninagawa, Yoshikazu Hattori, Hiroyuki Kumeta, Airu Takeuchi, Shinji Kajimoto, Hiroya Abe, Eiichiro Mori, Takahiro Muraoka, Takakazu Nakabayashi, Satoko Akashi, Tsukasa Okiyoneda, Michele Vendruscolo, Kenji Inaba, Masaki Okumura

    Nature Cell Biology　27　(11)　1952-1964　2025/11/11

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41556-025-01794-8 　

    ISSN： 1465-7392

    eISSN： 1476-4679

  8. Visualizing Thiol Stress Responses in Cells with a Water-Soluble Raman Sensor Peer-reviewed

    Hiroyuki Yamakoshi, Meichen Wang, Keisuke Koga, Shinji Kajimoto, Yuse Kuriyama, Yusuke Sasano, Takaaki Akaike, Yoshiharu Iwabuchi, Takakazu Nakabayashi

    Analytical Chemistry　97　(46)　25398-25405　2025/11/10

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.analchem.5c04391 　

    ISSN： 0003-2700

    eISSN： 1520-6882

  9. RNA-Mediated Inhibition Mechanism of Liquid–Liquid Phase Separation and Subsequent Aggregation Revealed by Raman Microscopy Peer-reviewed

    Taisei Ogura, Uchu Matsuura, Masato Machida, Kaichi Nagai, Shinji Kajimoto, Shinya Tahara, Takakazu Nakabayashi

    JACS Au　5　(11)　5749-5757　2025/11/03

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/jacsau.5c01234 　

    ISSN： 2691-3704

    eISSN： 2691-3704

  10. Label-free intracellular temperature measurement by integrating Raman imaging and deep learning Peer-reviewed

    Hiroaki Takahashi, Shinji Kajimoto, Ohsuke Irikura, Takakazu Nakabayashi

    Chemistry Letters　54　(3)　upaf032　2025/02/24

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/chemle/upaf032 　

    ISSN： 0366-7022

    eISSN： 1348-0715

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    Abstract We developed a label-free method for visualizing intracellular temperature distribution using Raman image analysis combined with deep learning. Analysis of the entire region of the O-H stretching band of water using deep learning achieves an accuracy of ∼0.2 °C, allowing intracellular temperature mapping without any pretreatments such as fluorescent labeling. Experiments using photoexcitation of carbon nanotubes attached to living cells demonstrate that this method can obtain transient changes in the temperature distribution within a cell.

  11. Nucleic Acid-Rich Stress Granules Are Not Merely Crowded Condensates: A Quantitative Raman Imaging Study Peer-reviewed

    Ren Shibuya, Shinji Kajimoto, Hideyuki Yaginuma, Tetsuro Ariyoshi, Yasushi Okada, Takakazu Nakabayashi

    Analytical Chemistry　96　17078-17085　2024/10

    DOI： 10.1021/acs.analchem.4c01096 　

  12. Raman quantitative analysis of methionine oxidation and its promotion effect on dissolution of phase-separated liquid droplets of Pbp1 Peer-reviewed

    Lisa Kageyama, Shinya Tahara, Kohei Yokosawa, Mana Kamijo, Shinji Kajimoto, Takakazu Nakabayashi

    Chemical Physics Letters　141671-141671　2024/10

    Publisher: Elsevier BV

    DOI： 10.1016/j.cplett.2024.141671 　

    ISSN： 0009-2614

  13. Establishment of a Method for the Introduction of Poorly Water-Soluble Drugs in Cells and Evaluation of Intracellular Concentration Distribution Using Resonance Raman Imaging Peer-reviewed

    Keisuke Koga, Shinji Kajimoto, Yuta Yoshizaki, Hiroaki Takahashi, Lisa Kageyama, Tomohiro Konno, Takakazu Nakabayashi

    The Journal of Physical Chemistry B　128　(6)　1350-1359　2024/01/31

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jpcb.3c06601 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  14. Highly sensitive Raman measurements of protein aqueous solutions using liquid–liquid phase separation

    Reona Tobita, Lisa Kageyama, Ayaka Saito, Shinya Tahara, Shinji Kajimoto, Takakazu Nakabayashi

    Chemical Communications　60　(66)　8732-8735　2024

    Publisher: Royal Society of Chemistry (RSC)

    DOI： 10.1039/d4cc03035d 　

    ISSN： 1359-7345

    eISSN： 1364-548X

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    A highly sensitive method is proposed for obtaining the Raman spectra of low-concentration proteins and nucleic acids in an aqueous solution using liquid–liquid phase separation.

  15. Visualization of the sequence of changes in the full wavenumber range by two-dimensional correlation spectroscopy Peer-reviewed

    Kazunori Ban, Daisuke Miyata, Yoshiteru Matsumoto, Shinya Tahara, Hiroaki Takahashi, Shin-ichi Morita, Takakazu Nakabayashi

    Chemical Physics Letters　140930-140930　2023/11

    Publisher: Elsevier BV

    DOI： 10.1016/j.cplett.2023.140930 　

    ISSN： 0009-2614

  16. Quantification of the concentration in a droplet formed by liquid–liquid phase separation of G-quadruplex-forming RNA Peer-reviewed

    Kohei Yokosawa, Mitsuki Tsuruta, Shinji Kajimoto, Naoki Sugimoto, Daisuke Miyoshi, Takakazu Nakabayashi

    Chemical Physics Letters　826　140634-140634　2023/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.cplett.2023.140634 　

    ISSN： 0009-2614

  17. Supersulphides provide airway protection in viral and chronic lung diseases Peer-reviewed

    Tetsuro Matsunaga, Hirohito Sano, Katsuya Takita, Masanobu Morita, Shun Yamanaka, Tomohiro Ichikawa, Tadahisa Numakura, Tomoaki Ida, Minkyung Jung, Seiryo Ogata, Sunghyeon Yoon, Naoya Fujino, Yorihiko Kyogoku, Yusaku Sasaki, Akira Koarai, Tsutomu Tamada, Atsuhiko Toyama, Takakazu Nakabayashi, Lisa Kageyama, Shigeru Kyuwa, Kenji Inaba, Satoshi Watanabe, Péter Nagy, Tomohiro Sawa, Hiroyuki Oshiumi, Masakazu Ichinose, Mitsuhiro Yamada, Hisatoshi Sugiura, Fan-Yan Wei, Hozumi Motohashi, Takaaki Akaike

    Nature Communications　14　(1)　4476　2023/07/25

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41467-023-40182-4 　

    eISSN： 2041-1723

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    Abstract Supersulphides are inorganic and organic sulphides with sulphur catenation with diverse physiological functions. Their synthesis is mainly mediated by mitochondrial cysteinyl-tRNA synthetase (CARS2) that functions as a principal cysteine persulphide synthase (CPERS). Here, we identify protective functions of supersulphides in viral airway infections (influenza and COVID-19), in aged lungs and in chronic lung diseases, including chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF). We develop a method for breath supersulphur-omics and demonstrate that levels of exhaled supersulphides increase in people with COVID-19 infection and in a hamster model of SARS-CoV-2 infection. Lung damage and subsequent lethality that result from oxidative stress and inflammation in mouse models of COPD, IPF, and ageing were mitigated by endogenous supersulphides production by CARS2/CPERS or exogenous administration of the supersulphide donor glutathione trisulphide. We revealed a protective role of supersulphides in airways with various viral or chronic insults and demonstrated the potential of targeting supersulphides in lung disease.

  18. In situ Quantification of Biomolecular Concentration of Cytoplasmic Membraneless Organelles in a Living Cell

    Ren Shibuya, Shinji Kajimoto, Hideyuki Yaginuma, Tetsuro Ariyoshi, Yasushi Okada, Takakazu Nakabayashi

    2023/05/16

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1101/2023.05.15.540722 　

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    ABSTRACT Liquid droplets formed via intracellular Liquid–liquid phase separation (LLPS) are called membraneless organelles and provide enzymatic reaction fields for maintaining cellular homeostasis, while they can be sources of protein aggregates and fibrils, causing neurodegenerative diseases. To understand the nature of intracellular liquid droplets, it is essential to quantify liquid droplets inside a living cell. Here, we performed near-IR fluorescence and Raman imaging to quantify chemical components inside stress granules (SGs) formed via LLPS in living cells under oxidative stress. The Raman images of stressed cells indicate the concentration of nucleic acids in the SGs was 20% higher than surrounding cytoplasm, while the lipid concentration was lower. The intensity of biomolecular C–H bands relative to the water band shows the net concentration of biomolecules was almost the same inside and outside the SGs, indicating intracellular droplets are not highly condensed, but the crowding environments are similar to the surroundings. TOC GRAPHICS

  19. Label-Free Tracking of Nanoprodrug Cellular Uptake and Metabolism Using Raman and Autofluorescence Imaging Peer-reviewed

    Masato Machida, Toshiki Sugimura, Shinji Kajimoto, Farsai Taemaitree, Yoshitaka Koseki, Hitoshi Kasai, Takakazu Nakabayashi

    The Journal of Physical Chemistry B　127　(17)　3851-3860　2023/04/24

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jpcb.3c01133 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  20. Label-free autofluorescence lifetime reveals the structural dynamics of ataxin-3 inside droplets formed via liquid–liquid phase separation Peer-reviewed

    Uchu Matsuura, Shinya Tahara, Shinji Kajimoto, Takakazu Nakabayashi

    Scientific Reports　13　(1)　6389　2023/04/19

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-023-33268-y 　

    eISSN： 2045-2322

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    Abstract Liquid–liquid phase separation is a phenomenon that features the formation of liquid droplets containing concentrated solutes. The droplets of neurodegeneration-associated proteins are prone to generate aggregates and cause diseases. To uncover the aggregation process from the droplets, it is necessary to analyze the protein structure with keeping the droplet state in a label-free manner, but there was no suitable method. In this study, we observed the structural changes of ataxin-3, a protein associated with Machado–Joseph disease, inside the droplets, using autofluorescence lifetime microscopy. Each droplet showed autofluorescence due to tryptophan (Trp) residues, and its lifetime increased with time, reflecting structural changes toward aggregation. We used Trp mutants to reveal the structural changes around each Trp and showed that the structural change consists of several steps on different timescales. We demonstrated that the present method visualizes the protein dynamics inside a droplet in a label-free manner. Further investigations revealed that the aggregate structure formed in the droplets differs from that formed in dispersed solutions and that a polyglutamine repeat extension in ataxin-3 hardly modulates the aggregation dynamics in the droplets. These findings highlight that the droplet environment facilitates unique protein dynamics different from those in solutions.

  21. Ratiometric analysis of reversible thia-Michael reactions using nitrile-tagged molecules by Raman microscopy Peer-reviewed

    Hiroyuki Yamakoshi, Daiki Shibata, Kazuki Bando, Shinji Kajimoto, Aki Kohyama, Syusuke Egoshi, Kosuke Dodo, Yoshiharu Iwabuchi, Mikiko Sodeoka, Katsumasa Fujita, Takakazu Nakabayashi

    Chemical Communications　59　14563-14566　2023

    Publisher: Royal Society of Chemistry (RSC)

    DOI： 10.1039/d3cc05015g 　

    ISSN： 1359-7345

    eISSN： 1364-548X

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    Ratiometric Raman analysis of reversible thia-Michael reactions was achieved using α-cyanoacrylic acid (αCNA) derivatives.

  22. Multiple deuteration of triphenylphosphine and live-cell Raman imaging of deuterium-incorporated Mito-Q Peer-reviewed

    Shogo Moriyama, Miyu Mae, Daiki Shibata, Hiroyuki Yamakoshi, Shinji Kajimoto, Takakazu Nakabayashi, Takayoshi Ishimoto, Kaiki Mogi, Hironao Sajiki, Shuji Akai, Yoshinari Sawama

    Chemical Communications　59　(81)　12100-12103　2023

    Publisher: Royal Society of Chemistry (RSC)

    DOI： 10.1039/d3cc04410f 　

    ISSN： 1359-7345

    eISSN： 1364-548X

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    All aromatic C–H bonds of triphenylphosphine (PPh3) were efficiently replaced by C–D bonds using Ru/C and Ir/C co-catalysts in 2-PrOH and D2O, an inexpensive deuterium source.

  23. SOD1 gains pro-oxidant activity upon aberrant oligomerization: change in enzymatic activity by intramolecular disulfide bond cleavage Peer-reviewed

    Kosuke Yamazaki, Shinya Tahara, Takumi Ohyama, Kunisato Kuroi, Takakazu Nakabayashi

    Scientific Reports　12　(1)　11750　2022/07/11

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-022-15701-w 　

    eISSN： 2045-2322

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    Abstract Copper-zinc superoxide dismutase (SOD1) has been proposed as one of the causative proteins of amyotrophic lateral sclerosis (ALS). The accumulation of non-native conformers, oligomers, and aggregates of SOD1 in motor neurons is considered responsible for this disease. However, it remains unclear which specific feature of these species induces the onset of ALS. In this study, we showed that disulfide-linked oligomers of denatured SOD1 exhibit pro-oxidant activity. Substituting all the cysteine residues in the free thiol state with serine resulted in the loss of both the propensity to oligomerize and the increase in pro-oxidant activity after denaturation. In contrast, these cysteine mutants oligomerized and acquired the pro-oxidant activity after denaturation in the presence of a reductant that cleaves the intramolecular disulfide bond. These results indicate that one of the toxicities of SOD1 oligomers is the pro-oxidant activity induced by scrambling of the disulfide bonds. Small oligomers such as dimers and trimers exhibit stronger pro-oxidant activity than large oligomers and aggregates, consistent with the trend of the cytotoxicity of oligomers and aggregates reported in previous studies. We propose that the cleavage of the intramolecular disulfide bond accompanied by the oligomerization reduces the substrate specificity of SOD1, leading to the non-native enzymatic activity.

  24. Concentration Quantification of the Low-Complexity Domain of Fused in Sarcoma inside a Single Droplet and Effects of Solution Parameters. International-journal Peer-reviewed

    Kohei Yokosawa, Shinji Kajimoto, Daiki Shibata, Kunisato Kuroi, Tomohiro Konno, Takakazu Nakabayashi

    The journal of physical chemistry letters　13　(24)　5692-5697　2022/06/23

    DOI： 10.1021/acs.jpclett.2c00962 　

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    Liquid-liquid phase separation (LLPS) is an important phenomenon in biology, and it is desirable to develop quantitative methods to analyze protein droplets generated by LLPS. This study quantified the change in protein concentration in a droplet in label-free and single-droplet conditions using Raman imaging and the Raman band of water as an intensity standard. Small changes in the protein concentration with variations in pH and salt concentration were observed, and it was shown that the concentration in the droplet decreases as the conditions become less favorable for droplet formation. The effect of exposure to 1,6-hexanediol was also examined, and this additive was found to decrease the protein concentration in the droplet. A model can be proposed in which the addition of 1,6-hexanediol reduces the protein concentration in the droplet, and the droplet disappears when the concentration falls below a certain threshold value.

  25. Regulation of Cell Volume by Nanosecond Pulsed Electric Fields Peer-reviewed

    Qi Yang, Shinji Kajimoto, Yuki Kobayashi, Hirotsugu Hiramatsu, Takakazu Nakabayashi

    The Journal of Physical Chemistry B　125　(38)　10692-10700　2021/09/30

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jpcb.1c06058 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  26. Label-free tracking of intracellular molecular crowding with cell-cycle progression using Raman microscopy Peer-reviewed

    Daiki Shibata, Shinji Kajimoto, Takakazu Nakabayashi

    Chemical Physics Letters　138843-138843　2021/06

    Publisher: Elsevier BV

    DOI： 10.1016/j.cplett.2021.138843 　

    ISSN： 0009-2614

  27. Observation of liquid–liquid phase separation of ataxin-3 and quantitative evaluation of its concentration in a single droplet using Raman microscopy Peer-reviewed

    Kazuki Murakami, Shinji Kajimoto, Daiki Shibata, Kunisato Kuroi, Fumihiko Fujii, Takakazu Nakabayashi

    Chemical Science　2021

    Publisher: Royal Society of Chemistry (RSC)

    DOI： 10.1039/d0sc06095j 　

    ISSN： 2041-6520

    eISSN： 2041-6539

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    <p>Liquid–liquid phase separation (LLPS) plays an important role in a variety of biological processes. We have established a method to quantify a single droplet formed by LLPS using the Raman band of water as an internal standard.</p>

  28. Automatic Determination of the Sequential Order of Dynamic Data and Its Application to Vibrational Spectroscopy Peer-reviewed

    Daisuke Miyata, Takakazu Nakabayashi, Shin-ichi Morita

    Journal of Chemical Information and Modeling　60　(10)　5070-5079　2020/10/26

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jcim.0c00627 　

    ISSN： 1549-9596

    eISSN： 1549-960X

  29. Pro-Oxidant Activity of an ALS-Linked SOD1 Mutant in Zn-Deficient Form. International-journal Peer-reviewed

    Chise Nagao, Kunisato Kuroi, Taiyu Wakabayashi, Takakazu Nakabayashi

    Molecules　25　(16)　2020/08/07

    DOI： 10.3390/molecules25163600 　

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    Cu, Zn superoxide dismutase (SOD1) is a representative antioxidant enzyme that catalyzes dismutation of reactive oxygen species in cells. However, (E,E)-SOD1 mutants in which both copper and zinc ions were deleted exhibit pro-oxidant activity, contrary to their antioxidant nature, at physiological temperatures, following denaturation and subsequent recombination of Cu2+. This oxidative property is likely related to the pathogenesis of amyotrophic lateral sclerosis (ALS); however, the mechanism by which Cu2+ re-binds to the denatured (E,E)-SOD1 has not been elucidated, since the concentration of free copper ions in cells is almost zero. In this study, we prepared the (Cu,E) form in which only a zinc ion was deleted using ALS-linked mutant H43R (His43→Arg) and found that (Cu,E)-H43R showed an increase in the pro-oxidant activity even at physiological temperature. The increase in the pro-oxidant activity of (Cu,E)-H43R was also observed in solution mimicking intracellular environment and at high temperature. These results suggest that the zinc-deficient (Cu,E) form can contribute to oxidative stress in cells, and that the formation of (E,E)-SOD1 together with the subsequent Cu2+ rebinding is not necessary for the acquisition of the pro-oxidant activity.

  30. Practical Computation of FFT-based Generalized Two-dimensional Correlation Spectroscopy Peer-reviewed

    Mengmeng HE, Daisuke MIYATA, Takakazu NAKABAYASHI, Shin-ichi MORITA

    Analytical Sciences　36　(7)　885-887　2020/07/10

    Publisher: Japan Society for Analytical Chemistry

    DOI： 10.2116/analsci.20n005 　

    ISSN： 0910-6340

    eISSN： 1348-2246

  31. Enhancement of Oxidative Reaction by the Intramolecular Electron Transfer between the Coordinated Redox-Active Metal Ions in SOD1. International-journal Peer-reviewed

    Takumi Ohyama, Kunisato Kuroi, Taiyu Wakabayashi, Nobuhiro Fujimaki, Takakazu Nakabayashi

    The journal of physical chemistry. B　124　(11)　2116-2123　2020/03/19

    DOI： 10.1021/acs.jpcb.9b11807 　

    More details Close

    The denatured Cu, Zn superoxide dismutase (SOD1) has the pro-oxidant activity that is suggested to be related with the pathogenesis of amyotrophic lateral sclerosis (ALS). We showed from the changes in the coordinated metal ions that the Cu ion in the Cu-binding site is the catalytic site of the pro-oxidant activity, and a redox-active metal ion in the Zn-binding site has the auxiliary function to enhance the pro-oxidant activity. The auxiliary function is suggested to arise from the intramolecular electron transfer between the coordinated metal ions in the denatured SOD1. The oxidation/reduction cycle of Cu in the Cu-binding site is assisted with changing the oxidation state of a metal ion in the Zn-binding site. The magnitude of the toxicity of the denatured SOD1 is discussed based on the ability of the auxiliary function.

  32. Automatic removal of binary background components expecting Raman big data and its application to human hair imaging Peer-reviewed

    Akunna Francess Ujuagu, Momoko Furuta, Takakazu Nakabayashi, Len Ito, Shin-Ichi Morita

    Applied Physics Express　13　(3)　036501-036501　2020/03/01

    Publisher: IOP Publishing

    DOI： 10.35848/1882-0786/ab6fb1 　

    ISSN： 1882-0778

    eISSN： 1882-0786

  33. A millisecond structured illumination microscope for super-resolution live cell imaging Peer-reviewed

    Tomu Suzuki, Shinji Kajimoto, Narufumi Kitamura, Mayumi Takano-Kasuya, Naoko Furusawa, Yasushi Nakano, Hiroshi Fukumura, Kohsuke Gonda, Takakazu Nakabayashi

    Applied Physics Express　13　(4)　045002 (4 page)　2020/03

    DOI： 10.35848/1882-0786/ab7cef 　

  34. Time-resolved FTIR study on the structural switching of human galectin-1 by light-induced disulfide bond formation. International-journal Peer-reviewed

    Kunisato Kuroi, Mana Kamijo, Mutsuki Ueki, Yusuke Niwa, Hirotsugu Hiramatsu, Takakazu Nakabayashi

    Physical chemistry chemical physics : PCCP　22　(3)　1137-1144　2020/01/22

    DOI： 10.1039/c9cp04881b 　

    More details Close

    Disulfide bonds play a fundamental role in controlling the tertiary structure of proteins; the formation or cleavage of some disulfide bonds can switch the structures and/or functions of proteins. Human galectin-1 (hGal-1), which is a lectin protein, exemplifies how both structure and function are changed by disulfide bonds; the structure and sugar-binding ability of hGal-1 are altered by the formation and cleavage of its three intra-molecular disulfide bonds. In the present study, the dynamics of the structural change of hGal-1 by the formation of disulfide bonds were investigated by time-resolved FTIR spectroscopy combined with a modification in which its thiol groups (-SH) were replaced with S-nitrosylated groups (SNO). Photodissociation of NO from SNO in reduced hGal-1 induced disulfide bond formation and transformed it into the oxidised form. The structural change to the oxidised form involved three distinct kinetics with fast (<300 s), middle (∼600 s), and slow (∼6400 s) lifetimes. In an examination of hGal-1 in the lactose-bound form, structural changes owing to the release of substrate lactose were also observed upon disulfide bond formation. The present method using the photodissociation of NO is useful for monitoring the dynamics of structural changes following disulfide formation.

  35. Observation of the changes in the chemical composition of lipid droplets using Raman microscopy Peer-reviewed

    Hiroaki Takahashi, Aya Yanamisawa, Shinji Kajimoto, Takakazu Nakabayashi

    Physical Chemistry Chemical Physics　2020

    Publisher: Royal Society of Chemistry (RSC)

    DOI： 10.1039/d0cp03805a 　

    ISSN： 1463-9076

    eISSN： 1463-9084

    More details Close

    <p>We report the dynamics of lipid droplet formation induced by introducing cis- and/or trans-fatty acids into cells. Raman imaging allows the chemical analysis of each droplet, showing that exogenous fatty...</p>

  36. Label‐Free Imaging of Intracellular Temperature by Using the O−H Stretching Raman Band of Water Peer-reviewed

    Toshiki Sugimura, Shinji Kajimoto, Takakazu Nakabayashi

    Angewandte Chemie International Edition　2020

    DOI： 10.1002/anie.201915846 　

  37. Effects of molecular crowding environment on the acquisition of toxic properties of wild-type SOD1 Peer-reviewed

    Takahashi A, Nagao C, Murakami K, Kuroi K, Nakabayashi T

    Biochimica et Biophysica Acta (BBA) - General Subjects　in press　2020

    DOI： 10.1016/j.bbagen.2019.07.010 　

  38. Application of IR spectra of two successive isotope labeled residues to the evaluation of dihedral angles of polyproline II structure Peer-reviewed

    Miyata D, Hiramatsu H, Nakabayashi T

    Chemical Physics Letters　718　27-31　2019

    DOI： 10.1016/j.cplett.2019.01.026 　

  39. Evaluation of Dihedral Angles of Peptides Using IR Bands of Two Successive Isotope Labeled Residues Peer-reviewed

    Okabe H, Miyata D, Nakabayashi T, Hiramatsu H

    Bulletin of the Chemical Society of Japan　92　(1)　80-86　2019

    DOI： 10.1246/bcsj.20180183 　

  40. Examination of the association states of dehydroergosterol towards understanding the association structures of sterols in a membrane Peer-reviewed

    Kuroi T, Imaizumi M, Miura T, Nakabayashi T

    Biochemical and Biophysical Research Communications　515　228-233　2019

    DOI： 10.1016/j.bbrc.2019.05.109 　

  41. Embedding a Metal-Binding Motif for Copper Transporter into a Lipid Bilayer by Cu(I) Binding Peer-reviewed

    Mariko Okada, Shinji Kajimoto, Takakazu Nakabayashi

    Journal of Physical Chemistry B　122　(24)　6364-6370　2018/06/21

    Publisher: American Chemical Society

    DOI： 10.1021/acs.jpcb.8b03179 　

    ISSN： 1520-5207 1520-6106

  42. Preparation and photo-induced activities of water-soluble amyloid β-C60 complexes Peer-reviewed

    Naoki Hasunuma, Masahiro Kawakami, Hirotsugu Hiramatsu, Takakazu Nakabayashi

    RSC Advances　8　(32)　17847-17853　2018

    Publisher: Royal Society of Chemistry

    DOI： 10.1039/c8ra02789g 　

    ISSN： 2046-2069

  43. Generalized Two-dimensional Correlation Analysis for Unimodal Waveforms Modeled by Quadratic Polynomials. Peer-reviewed

    Sugawara T, Nakabayashi T, Morita SI

    Analytical Sciences　34　(7)　845-847　2018

    Publisher: Japan Society for Analytical Chemistry

    DOI： 10.2116/analsci.17N040 　

    ISSN： 0910-6340

    eISSN： 1348-2246

  44. A Proposal for Automated Background Removal of Bio-Raman Data Peer-reviewed

    Tatsuro Sugawara, Qi Yang, Takakazu Nakabayashi, Shin-ichi Morita

    ANALYTICAL SCIENCES　33　(12)　1323-1325　2017/12

    DOI： 10.2116/analsci.33.1323 　

    ISSN： 0910-6340

    eISSN： 1348-2246

  45. Experimental Evaluation of the Density of Water in a Cell by Raman Microscopy Peer-reviewed

    Mizuki Takeuchi, Shinji Kajimoto, Takakazu Nakabayashi

    JOURNAL OF PHYSICAL CHEMISTRY LETTERS　8　(21)　5241-5245　2017/11

    DOI： 10.1021/acs.jpclett.7b02154 　

    ISSN： 1948-7185

  46. Change in the structure and function of lectin by photodissociation of NO Peer-reviewed

    T. Kurota, I. Sato, K. Kuroi, H. Hiramatsu, T. Nakabayashi

    CHEMICAL COMMUNICATIONS　53　(72)　10014-10017　2017/09

    DOI： 10.1039/c7cc04795a 　

    ISSN： 1359-7345

    eISSN： 1364-548X

  47. Effects of Nanosecond Pulsed Electric Field on Intracellular NADH Autofluorescence: A Comparison between Normal and Cancer Cells Peer-reviewed

    Kamlesh Awasthi, Takakazu Nakabayashi, Liming Li, Nobuhiro Ohta

    ACS Omega　2　(6)　2916-2924　2017/06/30

    Publisher: American Chemical Society

    DOI： 10.1021/acsomega.7b00315 　

    ISSN： 2470-1343

  48. Comparison of extracellular Cys/Trp motif between Schizosaccharomyces pombe Ctr4 and Ctr5 Peer-reviewed

    Mariko Okada, Takashi Miura, Takakazu Nakabayashi

    JOURNAL OF INORGANIC BIOCHEMISTRY　169　97-105　2017/04

    DOI： 10.1016/j.jinorgbio.2017.01.009 　

    ISSN： 0162-0134

    eISSN： 1873-3344

  49. Raman imaging microscopy for quantitative analysis of biological samples Peer-reviewed

    Shinji Kajimoto, Mizuki Takeuchi, Takakazu Nakabayashi

    Advances in Experimental Medicine and Biology　1035　163-172　2017

    Publisher: Springer New York LLC

    DOI： 10.1007/978-3-319-67358-5_12 　

    ISSN： 2214-8019 0065-2598

  50. Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell Peer-reviewed

    Takakazu Nakabayashi, Kamlesh Awasthi, Nobuhiro Ohta

    Advances in Experimental Medicine and Biology　1035　121-133　2017

    Publisher: Springer New York LLC

    DOI： 10.1007/978-3-319-67358-5_8 　

    ISSN： 2214-8019 0065-2598

  51. Sensitive detection of intracellular environment of normal and cancer cells by autofluorescence lifetime imaging Peer-reviewed

    Kamlesh Awasthi, Daiki Moriya, Takakazu Nakabayashi, Liming Li, Nobuhiro Ohta

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY　165　256-265　2016/12

    DOI： 10.1016/j.jphotobiol.2016.10.023 　

    ISSN： 1011-1344

  52. Acquisition of pro-oxidant activity of fALS-linked SOD1 mutants as revealed using circular dichroism and UV-resonance Raman spectroscopy Peer-reviewed

    Nobuhiro Fujimaki, Ken Nishiya, Takashi Miura, Takakazu Nakabayashi

    CHEMICAL PHYSICS　479　5-10　2016/11

    DOI： 10.1016/j.chemphys.2016.08.029 　

    ISSN： 0301-0104

    eISSN： 1873-4421

  53. Effects of Nanosecond Pulsed Electric Fields on the Intracellular Function of HeLa Cells As Revealed by NADH Autofluorescence Microscopy Peer-reviewed

    Kamlesh Awasthi, Takakazu Nakabayashi, Nobuhiro Ohta

    ACS Omega　1　(3)　396-406　2016/09/30

    DOI： 10.1021/acsomega.6b00090 　

    ISSN： 2470-1343

  54. Generation of self-clusters of galectin-1 in the farnesyl-bound form Peer-reviewed

    Kazumi Yamaguchi, Yusuke Niwa, Takakazu Nakabayashi, Hirotsugu Hiramatsu

    SCIENTIFIC REPORTS　6　32999　2016/09

    DOI： 10.1038/srep32999 　

    ISSN： 2045-2322

  55. The structural analysis of the pro-oxidant copper-binding site of denatured apo-H43R SOD1 and the elucidation of the origin of the acquisition of the pro-oxidant activity Peer-reviewed

    Nobuhiro Fujimaki, Takashi Miura, Takakazu Nakabayashi

    PHYSICAL CHEMISTRY CHEMICAL PHYSICS　18　(6)　4468-4475　2016/02

    DOI： 10.1039/c5cp07729j 　

    ISSN： 1463-9076

    eISSN： 1463-9084

  56. External Electric Field Effects on Excited-State Intramolecular Proton Transfer in 4 '-N,N-Dimethylamino-3-hydroxyflavone in Poly(methyl methacrylate) Films Peer-reviewed

    Kazuki Furukawa, Kazuyuki Hino, Norifumi Yamamoto, Kamlesh Awasthi, Takakazu Nakabayashi, Nobuhiro Ohta, Hiroshi Sekiya

    JOURNAL OF PHYSICAL CHEMISTRY A　119　(37)　9599-9608　2015/09

    DOI： 10.1021/acs.jpca.5b03672 　

    ISSN： 1089-5639

  57. Fluorescence Characteristics and Lifetime Images of Photosensitizers of Talaporfin Sodium and Sodium Pheophorbide a in Normal and Cancer Cells Peer-reviewed

    Kamlesh Awasthi, Kazuhito Yamamoto, Kazunari Furuya, Takakazu Nakabayashi, Liming Li, Nobuhiro Ohta

    SENSORS　15　(5)　11417-11430　2015/05

    DOI： 10.3390/s150511417 　

    ISSN： 1424-8220

  58. Redox sensor proteins for highly sensitive direct imaging of intracellular redox state Peer-reviewed

    Kazunori Sugiura, Takeharu Nagai, Masahiro Nakano, Hiroshi Ichinose, Takakazu Nakabayashi, Nobuhiro Ohta, Toru Hisabori

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　457　(3)　242-248　2015/02

    DOI： 10.1016/j.bbrc.2014.12.095 　

    ISSN： 0006-291X

    eISSN： 1090-2104

  59. DNA G-Quadruplex Detection System Employing a Protein Fibril Ligand Peer-reviewed

    Maeda R, Yaku H, Nakabayashi T, Murashima T, Sugimoto N, Ohta N, Miyoshi D

    Telomere and Telomerase　2　(1)　e691　2015

    DOI： 10.14800/tt.691 　

  60. Electric Field Effects on Photoluminescence of CdSe Nanoparticles in a PMMA Film Peer-reviewed

    Takakazu Nakabayashi, Ruriko Ohshima, Nobuhiro Ohta

    CRYSTALS　4　(2)　152-167　2014/06

    DOI： 10.3390/cryst4020152 　

    ISSN： 2073-4352

  61. Studies on external electric field effects on absorption and fluorescence spectra of NADH Peer-reviewed

    Takakazu Nakabayashi, Md. Serajul Islam, Liming Li, Masahide Yasuda, Nobuhiro Ohta

    CHEMICAL PHYSICS LETTERS　595　25-30　2014/03

    DOI： 10.1016/j.cplett.2014.01.035 　

    ISSN： 0009-2614

    eISSN： 1873-4448

  62. pH dependence of the fluorescence lifetime of FAD in solution and in cells Peer-reviewed

    Md. Serajul Islam, Masato Honma, Takakazu Nakabayashi, Masataka Kinjo, Nobuhiro Ohta

    International Journal of Molecular Sciences　14　(1)　1952-1963　2013

    DOI： 10.3390/ijms14011952 　

    ISSN： 1422-0067 1661-6596

  63. Application of Nanosecond Pulsed Electric Fields into He La Cells Expressing Enhanced Green Fluorescent Protein and Fluorescence Lifetime Microscopy Peer-reviewed

    Kamlesh Awasthi, Takakazu Nakabayashi, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY B　116　(36)　11159-11165　2012/09

    DOI： 10.1021/jp306550v 　

    ISSN： 1520-6106

  64. Changes in the electric dipole moments and molecular polarizabilities of enol and keto forms of 2-(2 '-hydroxyphenyl)benzimidazole along the proton transfer reaction path in a PMMA film Peer-reviewed

    Kazuki Furukawa, Norifumi Yamamoto, Takakazu Nakabayashi, Nobuhiro Ohta, Kiichi Amimoto, Hiroshi Sekiya

    CHEMICAL PHYSICS LETTERS　539　45-49　2012/06

    DOI： 10.1016/j.cplett.2012.05.021 　

    ISSN： 0009-2614

  65. pH dependence of the fluorescence lifetime of enhanced yellow fluorescent protein in solution and cells Peer-reviewed

    Takakazu Nakabayashi, Shugo Oshita, Ryoya Sumikawa, Fan Sun, Masataka Kinjo, Nobuhiro Ohta

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY　235　(1)　65-71　2012/05

    DOI： 10.1016/j.jphotochem.2012.02.016 　

    ISSN： 1010-6030

  66. pH-Dependent Network Formation of Quantum Dots and Fluorescent Quenching by Au Nanoparticle Embedding Peer-reviewed

    Shota Sekiguchi, Kenichi Niikura, Naoki Iyo, Yasutaka Matsuo, Asumi Eguchi, Takakazu Nakabayashi, Nobuhiro Ohta, Kuniharu Ijiro

    ACS APPLIED MATERIALS & INTERFACES　3　(11)　4169-4173　2011/11

    DOI： 10.1021/am201013n 　

    ISSN： 1944-8244

  67. Intracellular pH Sensing Using Autofluorescence Lifetime Microscopy Peer-reviewed

    Shinya Ogikubo, Takakazu Nakabayashi, Takashi Adachi, Md. Serajul Islam, Tomokazu Yoshizawa, Masataka Kinjo, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY B　115　(34)　10385-10390　2011/09

    DOI： 10.1021/jp2058904 　

    ISSN： 1520-6106

  68. External Electric Field Effects on State Energy and Photoexcitation Dynamics of Water-Soluble CdTe Nanoparticles Peer-reviewed

    Ruriko Ohshima, Takakazu Nakabayashi, Yoichi Kobayashi, Naoto Tamai, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY C　115　(31)　15274-15281　2011/08

    DOI： 10.1021/jp204660m 　

    ISSN： 1932-7447

  69. Electric-Field-Induced Changes in Absorption and Fluorescence of the Green Fluorescent Protein Chromophore in a PMMA Film Peer-reviewed

    Takakazu Nakabayashi, Kazuyuki Hino, Yuka Ohta, Sayuri Ito, Hirofumi Nakano, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY B　115　(26)　8622-8626　2011/07

    DOI： 10.1021/jp203090e 　

    ISSN： 1520-6106

  70. Electroabsorption spectra of PbSe nanocrystal quantum dots Peer-reviewed

    Xiaoming Liu, Toshifumi Iimori, Ruriko Ohshima, Takakazu Nakabayashi, Nobuhiro Ohta

    APPLIED PHYSICS LETTERS　98　(16)　161911-1-161911-3　2011/04

    DOI： 10.1063/1.3583450 　

    ISSN： 0003-6951

    eISSN： 1077-3118

  71. Fluorescence Decay Dynamics of Flavin Adenine Dinucleotide in a Mixture of Alcohol and Water in the Femtosecond and Nanosecond Time Range Peer-reviewed

    Takakazu Nakabayashi, Md. Serajul Islam, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY B　114　(46)　15254-15260　2010/11

    DOI： 10.1021/jp1063066 　

    ISSN： 1520-6106

  72. Fluorescence lifetime imaging spectroscopy in living cells with particular regards to pH dependence and electric field effect Peer-reviewed

    Nobuhiro Ohta, Takakazu Nakabayashi, Shugo Oshita, Masataka Kinjo

    Progress in Biomedical Optics and Imaging - Proceedings of SPIE　7576　2010

    DOI： 10.1117/12.841475 　

    ISSN： 1605-7422

  73. Fluorescence lifetime imaging spectroscopy in living cells with particular regards to pH dependence and electric field effect Peer-reviewed

    Nobuhiro Ohta, Takakazu Nakabayashi, Shugo Oshita, Masataka Kinjo

    REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS II　7576　2010

    DOI： 10.1117/12.841475 　

    ISSN： 0277-786X

  74. Electroabsorption and Electrophotoluminescence Spectra of Porphyrin Supramolecules in a Polymer Film Peer-reviewed

    Takakazu Nakabayashi, Takefumi Yotsutsuji, Kazuya Ogawa, Yoshiaki Kobuke, Nobuhiro Ohta

    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN　83　(1)　33-38　2010/01

    DOI： 10.1246/bcsj.20090264 　

    ISSN： 0009-2673

    eISSN： 1348-0634

  75. Fluorescence Lifetime Imaging Study on Living Cells with Particular Regard to Electric Field Effects and pH Dependence Peer-reviewed

    Nobuhiro Ohta, Takakazu Nakabayashi

    Molecular Nano Dynamics: Vol. I: Spectroscopic Methods and Nanostructures / Vol. II: Active Surfaces, Single Crystals and Single Biocells　2　607-621　2009/11/27

    Publisher: John Wiley and Sons

    DOI： 10.1002/9783527627820.ch31 　

  76. External Electric Field Effects on Emission of a Dendrimer-encapsulated Gold Nanocluster in a Polymer Film Peer-reviewed

    Ruriko Ohshima, Takakazu Nakabayashi, Tatsuo Hamada, Nobuhiro Ohta

    CHEMISTRY LETTERS　38　(6)　546-547　2009/06

    DOI： 10.1246/cl.2009.546 　

    ISSN： 0366-7022

    eISSN： 1348-0715

  77. Fluorescence lifetime imaging study of a single cell: Stress-induced environmental change and electric field effects onfluorescence Peer-reviewed

    Nobuhiro Ohta, Takakazu Nakabayashi, Issei Nagao, Masataka Kinjo, Yumiko Aoki, Minoru Tanaka

    Progress in Biomedical Optics and Imaging - Proceedings of SPIE　7190　2009

    DOI： 10.1117/12.808484 　

    ISSN： 1605-7422

  78. Fluorescence lifetime imaging study of a single cell: Stress-induced environmental change and electric field effects on fluorescence Peer-reviewed

    Nobuhiro Ohta, Takakazu Nakabayashi, Issei Nagao, Masataka Kinjo, Yumiko Aoki, Minoru Tanaka

    REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS　7190　2009

    DOI： 10.1117/12.808484 　

    ISSN： 0277-786X

  79. Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-alpha induced apoptosis Peer-reviewed

    Toshiyuki Ito, Shugo Oshita, Takakazu Nakabayashi, Fan Sun, Masataka Kinjo, Nobuhiro Ohta

    PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES　8　(6)　763-767　2009

    DOI： 10.1039/b902341k 　

    ISSN： 1474-905X

  80. Electric field effects on fluorescence of the green fluorescent protein Peer-reviewed

    Takakazu Nakabayashi, Masataka Kinjo, Nobuhiro Ohta

    CHEMICAL PHYSICS LETTERS　457　(4-6)　408-412　2008/05

    DOI： 10.1016/j.cplett.2008.04.018 　

    ISSN： 0009-2614

  81. Magnetic field effect on fluorescence in a mixture of N-ethylcarbazole and dimethyl terephthalate in a polymer film in tine presence of electric fields Peer-reviewed

    Kamlesh Awasthi, Miwako Mizoguchi, Toshifumi Iimori, Takakazu Nakabayashi, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY A　112　(19)　4432-4436　2008/05

    DOI： 10.1021/jp8001965 　

    ISSN： 1089-5639

  82. Studies on effects of external electric fields on halobacteria with fluorescence intensity and fluorescence lifetime imaging microscopy Peer-reviewed

    Takakazu Nakabayashi, Hui-Ping Wang, Kazuo Tsujimoto, Seiji Miyauchi, Naoki Kamo, Nobubiro Ohta

    CHEMISTRY LETTERS　37　(5)　522-523　2008/05

    DOI： 10.1246/cl.2008.522 　

    ISSN： 0366-7022

    eISSN： 1348-0715

  83. Direct measurements of the electric-field-induced change in fluorescence decay profile of a methylene-linked compound of pyrene Peer-reviewed

    Takakazu Nakabayashi, Takehiro Morikawa, Nobuhiro Ohta

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY　196　(2-3)　227-232　2008/05

    DOI： 10.1016/j.jphotochem.2007.07.026 　

    ISSN： 1010-6030

  84. Stress-induced environmental changes in a single cell as revealed by fluorescence lifetime imaging Peer-reviewed

    Takakazu Nakabayashi, Issei Nagao, Masataka Kinjo, Yumiko Aoki, Minoru Tanaka, Nobuhiro Ohta

    PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES　7　(6)　671-674　2008

    DOI： 10.1039/b805032e 　

    ISSN： 1474-905X

  85. Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements Peer-reviewed

    Takakazu Nakabayashi, Hui-Ping Wang, Masataka Kinjo, Nobuhiro Ohta

    PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES　7　(6)　668-670　2008

    DOI： 10.1039/b800391b 　

    ISSN： 1474-905X

  86. Electric field effects on absorption and fluorescence spectra of trimethylsilyl- and trimethylsilylethynyl-substituted compounds of pyrene in a PMMA film Peer-reviewed

    Anine Mane Ara, Toshifumi Iimori, Takakazu Nakabayashi, Haffirne Maeda, Kazuhiko Mizuno, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY B　111　(36)　10687-10696　2007/09

    DOI： 10.1021/jp073718a 　

    ISSN： 1520-6106

  87. Femtosecond time-resolved photo-absorption studies on the excitation dynamics of chromium(III) porphyrin complexes in solution Peer-reviewed

    Masahiko Inamo, Chie Okabe, Takakazu Nakabayashi, Nobuyuki Nishi, Mikio Hoshino

    CHEMICAL PHYSICS LETTERS　445　(4-6)　167-172　2007/09

    DOI： 10.1016/j.cplett.2007.07.079 　

    ISSN： 0009-2614

  88. External electric field effects on absorption, fluorescence, and phosphorescence spectra of diphenylpolyynes in a polymer film Peer-reviewed

    Md. Wahadoszamen, Tatsuo Hamada, Toshifumi Iimori, Takakazu Nakabayashi, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY A　111　(38)　9544-9552　2007/09

    DOI： 10.1021/jp073812r 　

    ISSN： 1089-5639

  89. Fluorescence lifetime image of a single halobacterium Peer-reviewed

    Hui-Ping Wang, Takakazu Nakabayashi, Kazuo Tsujimoto, Seiji Miyauchi, Naoki Kamo, Nobuhiro Ohta

    CHEMICAL PHYSICS LETTERS　442　(4-6)　441-444　2007/07

    DOI： 10.1016/j.cplett.2007.06.034 　

    ISSN： 0009-2614

  90. Molecular distortion effect on ff-emission in a Pr(III) complex with 4,7-diphenyl-1,10-phenanthroline Peer-reviewed

    Ayumi Ishii, Shinobu Kishi, Hideki Ohtsu, Toshifumi Iimori, Takakazu Nakabayashi, Nobuhiro Ohta, Naoto Tamai, Milan Melnik, Miki Hasegawa, Yuzo Shigesato

    CHEMPHYSCHEM　8　(9)　1345-1351　2007/06

    DOI： 10.1002/cphc.200700146 　

    ISSN： 1439-4235

  91. A correlation between pH and fluorescence lifetime of 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in vivo and in vitro Peer-reviewed

    Takakazu Nakabayashi, Hui-Ping Wang, Kazuo Tsujimoto, Seiji Miyauchi, Naoki Kamo, Nobuhiro Ohta

    CHEMISTRY LETTERS　36　(2)　206-207　2007/02

    DOI： 10.1246/cl.2007.206 　

    ISSN： 0366-7022

    eISSN： 1348-0715

  92. External electric field effects on fluorescence of pyrene butyric acid in a polymer film: Concentration dependence and temperature dependence Peer-reviewed

    Anjue Mane Ara, Toshifumi Iimori, Tomokazu Yoshizawa, Takakazu Nakabayashi, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY B　110　(47)　23669-23677　2006/11

    DOI： 10.1021/jp063865t 　

    ISSN： 1520-6106

  93. Electric-field-induced changes in absorption and emission spectra of CdS nanoparticles doped in a polymer film nanoparticles doped in a polymer film Peer-reviewed

    Yuhki Ohara, Takakazu Nakabayashi, Kentaro Iwasaki, Tsukasa Torimoto, Bunsho Ohtani, Takayuki Hiratani, Katsuaki Konishi, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY B　110　(42)　20927-20936　2006/10

    DOI： 10.1021/jp064205r 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  94. External electric field effects on absorption and fluorescence spectra of a fullerene derivative and its mixture with zinc-tetraphenylporphyrin doped in a PMMA film Peer-reviewed

    Md. Wahadoszamen, Takakazu Nakabayashi, Soonchul Kang, Hiroshi Imahori, Nobuhiro Ohta

    JOURNAL OF PHYSICAL CHEMISTRY B　110　(41)　20354-20361　2006/10

    DOI： 10.1021/jp0635967 　

    ISSN： 1520-6106

  95. External electric field effects on fluorescence of perylene doped in a polymer film Peer-reviewed

    Anjue Mane Ara, Toshifumi Iimori, Tomokazu Yoshizawa, Takakazu Nakabayashi, Nobuhiro Ohta

    CHEMICAL PHYSICS LETTERS　427　(4-6)　322-328　2006/08

    DOI： 10.1016/j.cplett.2006.07.001 　

    ISSN： 0009-2614

  96. Photo-switching behavior of CdS nanoparticles doped in a polymer film Peer-reviewed

    Y Ohara, T Nakabayashi, K Iwasaki, T Torimoto, B Ohtani, N Ohta

    COMPTES RENDUS CHIMIE　9　(5-6)　742-749　2006/05

    DOI： 10.1016/j.crci.2005.02.040 　

    ISSN： 1631-0748

    eISSN： 1878-1543

  97. Effects of electric and magnetic fields on fluorescence in electron donor and acceptor pairs of pyrene and N-methylphthalimide doped in a polymer film Peer-reviewed

    T Yoshizawa, M Mizoguchi, T Iimori, T Nakabayashi, N Ohta

    CHEMICAL PHYSICS　324　(1)　26-39　2006/05

    DOI： 10.1016/j.chemphys.2005.09.051 　

    ISSN： 0301-0104

  98. External electric field effects on absorption and fluorescence of anthracene-(CH2)(n)-naphthalene bichromophoric molecules doped in a polymer film Peer-reviewed

    T Nakabayashi, B Wu, T Morikawa, T Iimori, MB Rubin, S Speiser, N Ohta

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY　178　(2-3)　236-241　2006/03

    DOI： 10.1016/j.jphotochem.2005.10.025 　

    ISSN： 1010-6030

  99. Electroab sorption spectra of a complex formed between tetraphenyl-porphyrin and fullerene in a polymer film Peer-reviewed

    M Wahadoszamen, T Nakabayashi, N Ohta

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY　178　(2-3)　177-184　2006/03

    DOI： 10.1016/j.jphotochem.2005.06.019 　

    ISSN： 1010-6030

  100. External electric field effects on emission of a mixture of tetraphenylporphyrin and fullerene doped in a polymer film Peer-reviewed

    M Wahadoszamen, T Nakabayashi, N Ohta

    JOURNAL OF THE CHINESE CHEMICAL SOCIETY　53　(1)　85-92　2006/02

    ISSN： 0009-4536

    eISSN： 2192-6549

  101. 蛍光寿命イメージングシステムの製作と生体試料および高分子試料への応用 Peer-reviewed

    中林 孝和, 飯森 俊文, 金城 政孝, 太田 信廣

    分光研究　55　(1)　31-39　2006

    Publisher: The Spectroscopical Society of Japan

    DOI： 10.5111/bunkou.55.31 　

    ISSN： 0038-7002 1884-6785

    More details Close

    We have constructed a fluorescence lifetime imaging (FLIM) system to study the photo-induced dynamics of living cells. The output from a femtosecond mode-locked Ti: sapphire laser is frequency-doubled and focused onto the sample with an objective lens of a confocal microscope. The fluorescence decay of the sample is measured at each pixel of a scanning image. Each fluorescence lifetime is evaluated quickly using time-gating electronics, and the fluorescence lifetime image can be obtained within several minutes. We have combined the FLIM system with a picosecond time-correlated single-photon counting system in which a microchannel-plate photomultiplier is used for detection, so that the value of the fluorescence lifetime at a position can be analyzed quantitatively. A high contrast light-scattering image of a polymer film is also found to be obtained with the FLIM system.

  102. Time-resolved measurements of the external electric field effects on fluorescence in electron donor and acceptor pairs of N-ethylcarbazole and dimethyl terephthalate doped in a polymer film Peer-reviewed

    T Iimori, T Yoshizawa, T Nakabayashi, N Ohta

    CHEMICAL PHYSICS　319　(1-3)　101-110　2005/12

    DOI： 10.1016/j.chemphys.2005.03.035 　

    ISSN： 0301-0104

  103. Electric field effects on IR absorption of alpha-helical polypeptide Peer-reviewed

    T Nakabayashi, N Ohta

    CHEMISTRY LETTERS　34　(8)　1194-1195　2005/08

    DOI： 10.1246/cl.2005.1194 　

    ISSN： 0366-7022

    eISSN： 1348-0715

  104. External electric field effects on state energy and photoexcitation dynamics of diphenylpolyenes Peer-reviewed

    T Nakabayashi, M Wahadoszamen, N Ohta

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　127　(19)　7041-7052　2005/05

    DOI： 10.1021/ja0401444 　

    ISSN： 0002-7863

  105. Remarkable temperature dependence of electric field-induced change in fluorescence spectra of pyrene doped in a polymer matrix Peer-reviewed

    T Iimori, AM Ara, T Yoshizawa, T Nakabayashi, N Ohta

    CHEMICAL PHYSICS LETTERS　402　(1-3)　206-211　2005/01

    DOI： 10.1016/j.cplett.2004.12.027 　

    ISSN： 0009-2614

  106. External electric field effects on absorption, fluorescence and phosphorescence spectra of 4-(dimethylamino)benzonitrile in a polymer film Peer-reviewed

    T Yoshizawa, Y Iwaki, N Osaka, T Nakabayashi, KA Zachariasse, N Ohta

    JOURNAL OF PHYSICAL CHEMISTRY B　108　(50)　19132-19139　2004/12

    DOI： 10.1021/jp040012x 　

    ISSN： 1520-6106

  107. Ultrafast excited-state dynamics in photochromic N-salicylideneaniline studied by femtosecond time-resolved REMPI spectroscopy Peer-reviewed

    C Okabe, T Nakabayashi, Y Inokuchi, N Nishi, H Sekiya

    JOURNAL OF CHEMICAL PHYSICS　121　(19)　9436-9442　2004/11

    DOI： 10.1063/1.1801991 　

    ISSN： 0021-9606

  108. Direct measurements of the electric-field-induced change in fluorescence decay profile of pyrene doped in a polymer film Peer-reviewed

    T Nakabayashi, T Morikawa, N Ohta

    CHEMICAL PHYSICS LETTERS　395　(4-6)　346-350　2004/09

    DOI： 10.1016/j.cplett.2004.07.103 　

    ISSN： 0009-2614

  109. Electric field effects on photoisomerization process of diphenylpolyenes doped in a polymer film as revealed by a field-induced change in fluorescence spectrum Peer-reviewed

    M Wahadoszamen, T Nakabayashi, N Ohta

    CHEMICAL PHYSICS LETTERS　387　(1-3)　124-129　2004/03

    DOI： 10.1016/j.cplett.2004.01.107 　

    ISSN： 0009-2614

  110. A relation between external electric field effect on photoluminescence and generation efficiency of electroluminescence: methylene-linked compounds of pyrene doped in a polymer film Peer-reviewed

    N. Ohta, T. Nakabayashi, T. Iimori, T. Yoshizawa, T. Morikawa

    Proceedings of Electroluminescence Conference　2004　358-361　2004

  111. Picosecond time-resolved stokes and anti-stokes Raman studies on the photochromic reactions of diarylethene derivatives Peer-reviewed

    C Okabe, T Nakabayashi, N Nishi, T Fukaminato, T Kawai, M Irie, H Sekiya

    JOURNAL OF PHYSICAL CHEMISTRY A　107　(28)　5384-5390　2003/07

    DOI： 10.1021/jp035055c 　

    ISSN： 1089-5639

  112. States of molecular associates in binary mixtures of acetic acid with protic and aprotic polar solvents: A Raman spectroscopic study Peer-reviewed

    T Nakabayashi, N Nishi

    JOURNAL OF PHYSICAL CHEMISTRY A　106　(14)　3491-3500　2002/04

    DOI： 10.1021/jp012606v 　

    ISSN： 1089-5639

  113. Observation of formation dynamics of solvated aromatic cation radicals following photoionization Peer-reviewed

    T Nakabayashi, S Kamo, K Watanabe, H Sakuragi, N Nishi

    CHEMICAL PHYSICS LETTERS　355　(3-4)　241-248　2002/04

    DOI： 10.1016/S0009-2614(02)00182-3 　

    ISSN： 0009-2614

  114. Time-resolved Raman studies of photoionization of aromatic compounds in polar solvents: Picosecond relaxation dynamics of aromatic cation radicals Peer-reviewed

    T Nakabayashi, S Kamo, H Sakuragi, N Nishi

    JOURNAL OF PHYSICAL CHEMISTRY A　105　(38)　8605-8614　2001/09

    DOI： 10.1021/jp0110105 　

    ISSN： 1089-5639

  115. Theoretical study on the structures and energies of acetic acid dimers in aqueous solution Peer-reviewed

    T Nakabayashi, H Sato, F Hirata, N Nishi

    JOURNAL OF PHYSICAL CHEMISTRY A　105　(1)　245-250　2001/01

    DOI： 10.1021/jp0030239 　

    ISSN： 1089-5639

  116. Time-resolved absorption studies on the photochromic process of 2H-benzopyrans in the picosecond to submillisecond time domain Peer-reviewed

    Y Kodama, T Nakabayashi, K Segawa, E Hattori, M Sakuragi, N Nishi, H Sakuragi

    JOURNAL OF PHYSICAL CHEMISTRY A　104　(49)　11478-11485　2000/12

    DOI： 10.1021/jp002032e 　

    ISSN： 1089-5639

  117. Transient vibrational temperatures estimated from anti-Stokes and Stokes Raman intensities and vibrational cooling of electronically excited trans-stilbene in solution Peer-reviewed

    H Okamoto, T Nakabayashi, M Tasumi

    JOURNAL OF RAMAN SPECTROSCOPY　31　(4)　305-309　2000/04

    DOI： 10.1002/(SICI)1097-4555(200004)31:4<305::AID-JRS543>3.0.CO;2-0 　

    ISSN： 0377-0486

  118. Raman spectroscopic study on acetic acid clusters in aqueous solutions: Dominance of acid-acid association producing microphases Peer-reviewed

    N Nishi, T Nakabayashi, K Kosugi

    JOURNAL OF PHYSICAL CHEMISTRY A　103　(50)　10851-10858　1999/12

    DOI： 10.1021/jp9929061 　

    ISSN： 1089-5639

  119. Liquid structure of acetic acid studied by Raman spectroscopy and ab initio molecular orbital calculations Peer-reviewed

    T Nakabayashi, K Kosugi, N Nishi

    JOURNAL OF PHYSICAL CHEMISTRY A　103　(43)　8595-8603　1999/10

    DOI： 10.1021/jp991501d 　

    ISSN： 1089-5639

  120. Picosecond anti-Stokes Raman excitation profiles as a method for investigating vibrationally excited transients Peer-reviewed

    H Okamoto, T Nakabayashi, M Tasumi

    LASER CHEMISTRY　19　(1-4)　335-341　1999

    ISSN： 0278-6273

  121. Pump- and probe-wavelength dependencies of picosecond anti-Stokes Raman spectrum of trans-stilbene in the S-1 state Peer-reviewed

    T Nakabayashi, H Okamoto, M Tasumi

    LASER CHEMISTRY　19　(1-4)　75-78　1999

    ISSN： 0278-6273

  122. Vibrational relaxation dynamics of trans-stilbene in the lowest excited singlet state. Pump and probe wavelength dependencies of the picosecond time-resolved anti-Stokes Raman spectrum Peer-reviewed

    T Nakabayashi, H Okamoto, M Tasumi

    JOURNAL OF PHYSICAL CHEMISTRY A　102　(48)　9686-9695　1998/11

    DOI： 10.1021/jp982287g 　

    ISSN： 1089-5639

  123. Sub-picosecond excited-state dynamics of a carotenoid (spirilloxanthin) in the light-harvesting systems of Chromatium vinosum. Relaxation process from the optically allowed S-2 state Peer-reviewed

    H Okamoto, M Ogura, T Nakabayashi, M Tasumi

    CHEMICAL PHYSICS　236　(1-3)　309-318　1998/09

    DOI： 10.1016/S0301-0104(98)00207-9 　

    ISSN： 0301-0104

  124. Low-frequency Raman spectra of crystalline and liquid acetic acid and its mixtures with water. Is the liquid dominated by hydrogen-bonded cyclic dimers? Peer-reviewed

    K Kosugi, T Nakabayashi, N Nishi

    CHEMICAL PHYSICS LETTERS　291　(3-4)　253-261　1998/07

    DOI： 10.1016/S0009-2614(98)00630-7 　

    ISSN： 0009-2614

  125. Probe-wavelength dependency of picosecond anti-stokes Raman spectra of trans-stilbene in the S-1 state Peer-reviewed

    T Nakabayashi, H Okamoto, M Tasumi

    JOURNAL OF PHYSICAL CHEMISTRY A　101　(39)　7189-7193　1997/09

    DOI： 10.1021/jp971495q 　

    ISSN： 1089-5639

  126. Analysis of anti-Stokes resonance Raman excitation profiles as a method for studying vibrationally excited molecules Peer-reviewed

    H Okamoto, T Nakabayashi, M Tasumi

    JOURNAL OF PHYSICAL CHEMISTRY A　101　(19)　3488-3493　1997/05

    DOI： 10.1021/jp963302p 　

    ISSN： 1089-5639

  127. Probe-wavelength dependence of picosecond time-resolved anti-Stokes Raman spectrum of canthaxanthin: Determination of energy states of vibrationally excited molecules generated via internal conversion from the lowest excited singlet state Peer-reviewed

    T Nakabayashi, H Okamoto, M Tasumi

    JOURNAL OF PHYSICAL CHEMISTRY A　101　(19)　3494-3500　1997/05

    DOI： 10.1021/jp963303h 　

    ISSN： 1089-5639

  128. PICOSECOND TRANSIENT RAMAN-SPECTRA OF PHOTOEXCITED 4-DIMETHYLAMINO-4'-NITROSTILBENE IN POLAR-SOLVENTS Peer-reviewed

    T NAKABAYASHI, H OKAMOTO, M TASUMI

    JOURNAL OF RAMAN SPECTROSCOPY　26　(8-9)　841-845　1995/08

    ISSN： 0377-0486

  129. TIME-RESOLVED PUMP-PROBE RAMAN-SPECTROSCOPY WITH TEMPORALLY INCOHERENT-LIGHT Peer-reviewed

    H OKAMOTO, T NAKABAYASHI, M TASUMI

    JOURNAL OF RAMAN SPECTROSCOPY　25　(7-8)　631-639　1994/07

    ISSN： 0377-0486

  130. Incoherent time-resolved pump-probe Raman spectroscopy Peer-reviewed

    H OKAMOTO, T NAKABAYASHI, M TASUMI

    FOURTEENTH INTERNATIONAL CONFERENCE ON RAMAN SPECTROSCOPY　480-481　1994

  131. INCOHERENT TIME-RESOLVED PUMP-PROBE RAMAN-SPECTROSCOPY Peer-reviewed

    H OKAMOTO, T NAKABAYASHI, M TASUMI

    JOURNAL OF PHYSICAL CHEMISTRY　97　(39)　9871-9873　1993/09

    DOI： 10.1021/j100141a001 　

    ISSN： 0022-3654

Show all ︎Show first 5

Misc. 39

  1. ラマンイメージングによる細胞内液液相分離の定量 Invited

    梶本 真司, 澁谷 蓮, 中林 孝和

    生物物理　66　154-158　2026/06

  2. Liquid–Liquid Phase Separation from the Viewpoint of Molecular Crowding Environment: A Raman Imaging Study Invited Peer-reviewed

    Takakazu Nakabayashi, Shinya Tahara, Shinji Kajimoto

    The Journal of Physical Chemistry B　129　8087-8098　2025/08/04

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jpcb.5c02288 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  3. リン脂質ポリマーを用いた難水溶性薬物分子の高効率細胞内導入と光を用いたラベルフリー濃度定量法の確立 Invited Peer-reviewed

    古賀圭祐, 梶本真司, 金野智浩, 中林孝和

    Photomedicine and Photobiology　46　9-14　2025

  4. ラマン顕微鏡を用いた相分離液滴のラベルフリー濃度定量 Invited

    澁谷蓮, 中林孝和, 梶本真司

    医学のあゆみ　292　268-274　2025

  5. Capturing the Natural States of Biomolecules in Crowded Environments: Label-free in situ Quantitative Analysis Using Raman Microscopy Invited Peer-reviewed

    Takakazu Nakabayashi

    Molecular Science　19　(1)　A0142-A0142　2025

    Publisher: Japan Society for Molecular Science

    DOI： 10.3175/molsci.19.a0142 　

    eISSN： 1881-8404

  6. ラマンイメージングによる脂肪滴形成過程のラベルフリー可視化 Invited

    高橋大智, 梶本真司, 中林孝和

    The Lipid　35　102-106　2024

  7. 酸化促進性の獲得からみSOD1の毒性発現機構 Invited

    田原 進也, 中林孝和

    ファルマシア　60　398-403　2024

  8. ラマンイメージングを用いた細胞内の水・夾雑環境の理解 Invited

    Takakazu Nakabayashi, Shinji Kajimoto

    100　(7)　367-370　2022/07

    DOI： 10.34565/seibutsukogaku.100.7_367 　

  9. ラマン顕微鏡によるラベルフリーなタンパク質液滴定量法 Invited

    中林孝和, 黒井邦巧, 梶本真司

    生化学　94　444-448　2022

  10. ラマン分光による細胞の中の水の計測と分子夾雑環境・温度イメージングへの応用 Invited

    中林孝和, 梶本真司

    光学　51　238-242　2022

  11. ラマン顕微鏡で細胞内の水を見る Invited

    中林孝和, 梶本真司

    化学と工業　75　209　2022

  12. 量子化学：硫黄代謝物の構造とラマン分光法を用いた検出 Invited

    影山莉沙, 中林孝和

    実験医学　39　2103-2106　2021

  13. 量子化学計算・ラマン分光法を用いた硫黄化合物の構造解析の動向 Invited

    影山莉沙, 中林孝和

    生化学　93　621-627　2021

  14. ラマン顕微鏡を用いた疾病関連タンパク質の液-液相分離の定量評価 Invited

    村上一輝, 梶本真司, 中林孝和

    細胞　53　548-551　2021

  15. 水による細胞内夾雑環境の定量評価とその応用 Invited

    柴田大輝, 梶本真司, 中林孝和

    細胞　53　370-372　2021

  16. 生きた細胞のなかの分子を直接見る Invited

    Shinji Kajimoto, Takakazu Nakabayashi

    Chemistry　75　(7)　64-65　2020/07

  17. 細胞内の水を用いたラベルフリー細胞内温度計測 Invited

    杉村俊紀, 梶本真司, 中林孝和

    細胞　52　20-21　2020

  18. 疾病の発症における分子狭雑環境と液液相分離の役割 Invited

    中林孝和, 横澤公平, 黒井邦巧

    化学工業　71　216-222　2020

  19. 細胞の水の密度を観る Invited

    梶本真司, 竹内瑞貴, 杉村俊紀, 中林孝和

    生物物理　59　97-99　2019

  20. バイオ・ラマン研究の現状とこれから Invited Peer-reviewed

    宮田 大輔, 中林 孝和, 盛田 伸一

    レーザー研究　47　85-88　2019

  21. 共焦点レーザー走査顕微鏡: V. FLIM (タイムドメイン) Invited

    NAKABAYASHI Takakazu

    実験医学増刊　36　(20)　55-56　2018

  22. 蛍光寿命イメージングの基礎と細胞内環境計測への応用 Invited

    中林孝和, 太田信廣

    バイオマテリアル 生体材料　34　(3)　210-215　2016

    Publisher: 日本バイオマテリアル学会 ; 2002-

    ISSN： 1347-7080

  23. Sensing of Intracellular Environments by Fluorescence Lifetime Imaging of Exogenous Fluorophores Invited Peer-reviewed

    Takakazu Nakabayashi, Nobuhiro Ohta

    ANALYTICAL SCIENCES　31　(4)　275-285　2015/04

    DOI： 10.2116/analsci.31.275 　

    ISSN： 0910-6340

    eISSN： 1348-2246

  24. NADHの自家蛍光寿命イメージングを用いた細胞内環境計測 Invited

    中林孝和, 太田信廣

    光学　44　(6)　221-226　2015

    Publisher: 日本光学会

    ISSN： 0389-6625

  25. 自家蛍光寿命イメージングによる細胞観察 Invited Peer-reviewed

    中林孝和, 太田信廣

    生物物理　53　(3)　166-169　2014

    Publisher: The Biophysical Society of Japan General Incorporated Association

    DOI： 10.2142/biophys.53.166 　

    ISSN： 0582-4052

  26. ラベルフリー細胞内自家蛍光寿命イメージング研究の進展 Invited

    中林孝和, 太田信廣

    化学と工業　67　(3)　262-262　2014

  27. 自家蛍光寿命イメージングの基礎と展開 Invited

    中林孝和, 太田信廣

    光化学　42　(2)　52-59　2011/04

  28. 蛍光寿命イメージングによる生体分析 Invited Peer-reviewed

    中林 孝和, 太田 信廣

    日本レーザー医学会誌　30　(4)　441-448　2010

  29. Studies on Microenvironment in a Single Cell Using Fluorescence Lifetime Imaging Microscopy Invited Peer-reviewed

    Takakazu Nakabayashi, Nobuhiro Ohta

    BUNSEKI KAGAKU　58　(6)　473-485　2009/06

    DOI： 10.2116/bunsekikagaku.58.473 　

    ISSN： 0525-1931

  30. 共焦点顕微鏡と半透明金属基板を用いた反射・散乱光画像計測 Invited Peer-reviewed

    中林 孝和, 太田 信廣

    分光研究　58　(6)　275-276　2009

    ISSN： 0038-7002

  31. 蛍光寿命を用いた生体試料の画像観測 Invited

    中林孝和, 太田信廣

    ぶんせき　597-600　2007/04

  32. 蛍光寿命イメージングによる単一細胞内の生体分子間の相互作用の観察 Invited Peer-reviewed

    中林 孝和

    分光研究　54　(4)　256-257　2005

    Publisher: The Spectroscopical Society of Japan

    DOI： 10.5111/bunkou.54.256 　

    ISSN： 0038-7002 1884-6785

  33. 高速時間分解分光による孤立分子および分子集合体のフォトクロミック機構の解明 Invited

    関谷博, 入江正浩, 迫田憲治, 深港豪, 大坪暢人, 田中伸幸, 西信之, 岡部智絵, 中林孝和, 井口佳哉, 河合壮

    分子研レターズ　50　80-89　2004

  34. 水のクラスター構造 −基本的な考え方と応用の可能性− Invited

    中林 孝和

    食品膜技術懇談会誌　29　(29)　35-40　2003

    Publisher: 食品膜・分離技術研究会事務局

  35. 液体アルコール分子の分子内振動エネルギー移動の実時間観測 Invited Peer-reviewed

    中林 孝和

    化学と工業　56　(10)　1147-1147　2003

    ISSN： 0022-7684

  36. 化学物質が溶けること・混ざること Invited

    中林 孝和

    ガイア　10　3-3　2002

  37. Photochemistry of Photochromic Benzopyrans Studied by Time-Resolved Absorption Spectroscopy Invited Peer-reviewed

    T. Nakabayashi, N. Nishi, H. Sakuragi

    Science Progress　84　(2)　137-156　2001

  38. 溶液中での局所構造とクラスターレベルで見た混合状態 Invited

    中林孝和, 西信之

    先端ウオッチング報告書　5　87-92　2001

  39. 中赤外領域でのピコ・フェムト秒分光 Invited Peer-reviewed

    中林孝和

    化学と工業　48　1268　1995/04

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Books and Other Publications 11

  1. 実験医学別冊「相分離の解析プロトコール「それ本当に相分離」と言われないための戦略と先端解析方法」

    梶本真司, 柴田大輝, 中林孝和

    2022

  2. ラマン分光スペクトル解析事例集

    中林孝和, 柴田大輝, 高橋大智, 梶本真司

    技術情報協会　2022

  3. 先端の分析法 第2版

    中林孝和

    2022

  4. Prime Archives in Sensors

    Kamlesh Awasthi, Kazuhito Yamamoto, Kazunari Furuya, Takakazu Nakabayashi, Liming Li, Nobuhiro Ohta

    2020

  5. 現代化学増刊46 相分離生物学の全貌

    中林孝和, 黒井邦巧

    2020

  6. Multi-Parametric Live Cell Microscopy of 3D Tissue Models

    Kajimoto S, Takeuchi M, Nakabayashi T

    Springer　2017

  7. 光と生命の事典

    NAKABAYASHI Takakazu

    朝倉書店　2016

  8. 発光の事典

    中林孝和

    朝倉書店　2015

  9. Natural Biomarkers for Cellular Metabolism: Biology, Techniques, and Applications

    Ohta N, Nakabayashi, T

    CRC Press　2014

  10. 光化学の事典– 誰でもわかる光化学の初歩 –

    中林孝和

    朝倉書店　2014

  11. Molecular Nanodynamics

    Ohta N, Nakabayashi, T

    Wiley-VCH　2009

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Presentations 102

  1. ラマン顕微鏡を用いたその場定量解析の生体高分子の液滴・水和水への応用 Invited

    中林孝和

    高分子学会講演会 相分離液滴を基盤とするバイオマテリアル・デバイス開発　2026/07

  2. ラマンイメージングを用いた液 - 液相分離による液滴の構成成分のその場定量計測 Invited

    中林孝和

    第26回日本蛋白質科学年会　2026/06

  3. 水のラマン可視化で解く細胞内・生体高分子の水環境およびタンパク質の液-液相分離 Invited

    中林孝和

    第1回豊田理研フェロー交流会ワークショップ　2026/03

  4. ラマン分光の深化：生体分子・時間分解解析から細胞内定量イメージングへ Invited

    中林孝和

    シンポジウム：先端光科学研究の新展開　2026/03

  5. The role of RNAs in droplet formation and dissipation: Raman microscopic studies Invited

    Takakazu Nakabayashi

    LLPS symposium 2025　2025/11

  6. “水”を標的としたラマン可視化：細胞内・生体高分子の水環境およびタンパク質の液-液相分離・凝集化の理解 Invited

    中林孝和

    分子研コロキウム　2025/09

  7. Raman bands of water: Quantifying water density and biomolecules in a liquid droplet and a living cell Invited

    Takakazu Nakabayashi

    Frontiers in Water Biophysics　2025/07

  8. ラマン顕微鏡による細胞内の超硫黄分子とタンパク質の超硫黄構造のラベルフリー検出 Invited

    日本薬学会第145年会 シンポジウムレドックスバイオロジーのためのイメージング・検出技術の最前線　2025/03

  9. Label-free Detection of Supersulfides and Their Metabolic Reactions in a Cell Using Raman Imaging Invited

    Takakazu Nakabayashi

    17h International Symposium on Nanomedicine　2024/12

  10. Highly Sensitive Raman Measurements of Biomolecules in a Liquid Droplet formed by Liquid–Liquid Phase Separation Invited

    Takakazu Nakabayashi

    International Conference on Optics Within Life Sciences (OWLS-17)　2024/11

  11. 先端分光法を用いて細胞の中のドロドロした環境と神経変性疾患発症との関係を探る Invited

    中林孝和

    日本大学工学部大学院特別講義　2024/10

  12. In situ label-free quantification of molecular concentrations in a single living cell using Raman imaging Invited

    Takakazu Nakabayashi

    令和6年度化学系学協会東北大会（分析化学・環境化学セッション）　2024/09

  13. ラマンイメージングを用いた液滴内に多量に存在する核酸の発見と液滴消滅および凝集化における核酸の役割 Invited

    中林孝和

    第24回日本蛋白質科学年会　2024/06

  14. 分子分光学的手法を駆使した生体分子および細胞内現象のその場解析 Invited

    中林孝和

    日本化学会第104春季年会学術賞受賞講演　2024/03

  15. 細胞の中のドロドロとした環境としての液-液相分離：ラマンイメージングからわかること Invited

    中林孝和

    ニコンシリーズオンラインセミナー「相分離」第4回　2024/03

  16. ラマン顕微鏡の応用：光を使って単一細胞内の薬剤・生体分子の濃度をその場定量する Invited

    中林孝和

    日本薬学会第144年会シンポジウム 光を用いた次世代Photo Bio Medicine　2024/03

  17. 細胞内の液滴の中に何があるのか、どのくらいあるのか、どうしてあるのかをラマンイメージングで探る Invited

    中林孝和

    生体分子凝縮体勉強会　2023/12

  18. Quantification of Intracellular Molecules in a Living Cell Using Raman Imaging Invited

    Takakazu Nakabayashi

    16h International Symposium on Nanomedicine　2023/10

  19. ラマンイメージングを用いた濃度定量法の構築と細胞内分子・液液相分離への応用 Invited

    中林孝和

    医用分光学研究会第21回年会　2023/10

  20. 細胞内分子の濃度を直接測る～細胞内混雑環境とタンパク質液滴との関係～ Invited

    中林孝和

    第13回CSJ化学フェスタ2023　2023/10

  21. Measurements of Water in a Living Cell: Application to Evaluate Intracellular Environments Invited

    Takakazu Nakabayashi

    GP-Chem Kickoff Symposium　2023/09

  22. 水のラマンバンドを用いた細胞・タンパク質のその場定量解析 Invited

    中林孝和

    第45回日本光医学・光生物学会　2023/06

  23. 細胞の中の水のイメージング Invited

    中林孝和

    第17回日本分子イメージング学会学術集会　2023/06

  24. Application of Water Raman Bands to Evaluate Intracellular Environments and Liquid-Liquid Phase Separation Invited

    Takakazu Nakabayashi

    The Eighth Taiwan International Symposium on Raman Spectroscopy　2023/06

  25. 単一液滴内のラベルフリー濃度測定からみた液液相分離と分子夾雑環境 Invited

    中林孝和

    第二回相分離生物物理学研究会　2023/03

  26. 分子夾雑環境の一つとしての液液相分離：ラマン顕微鏡によるラベルフリー濃度測定 Invited

    中林孝和

    日本化学会コロイド先端技術講座2022ソフトマターの「液液」相分離　2023/03

  27. ラマンイメージングを用いた細胞内の水計測とその場定量への応用 Invited

    中林孝和

    電子情報通信学会2023年総合大会シンポジウム　2023/03

  28. Investigation of chemical properties of a single protein droplet formed by liquid-liquid phase separation Invited

    Takakazu Nakabayashi

    15h International Symposium on Nanomedicine　2022/12

  29. Water in a living cell probed by a Raman microscopy Invited

    Takakazu Nakabayashi

    EMLG/JMLG 2022 Annual Meeting　2022/09

  30. 細胞・タンパク質のラベルフリーイメージングでわかったこと・これからの課題 Invited

    中林孝和

    第31回日本バイオイメージング学会　2022/09

  31. Dynamics and structural changes associated with liquid-liquid phase separation in neurodegenerative disease-related proteins Invited

    Takakazu Nakabayashi

    The 7th Japan-Taiwan Joint Symposium for Pharmaceutical Sciences　2022/08

  32. 水のラマンイメージングからみた細胞内環境・細胞内温度・液−液相分離 Invited

    中林孝和

    日本分光学会関西支部 2022年度第1回講演会　2022/07

  33. ラマンイメージングを用いた液液相分離・分子夾雑環境の定量 Invited

    中林孝和

    第22回日本蛋白質科学会年会　2022/07

  34. ラマン顕微鏡を用いた硫黄・細胞内状態計測 Invited

    中林孝和

    第2回レドックスR&D戦略委員会春のシンポジウム　2022/03

  35. ラマン分光を用いた水素結合性液体および細胞内にある水の状態解析 Invited

    中林孝和

    理研シンポジウム 「量子ビームで創る新しい生命科学：X線からテラヘルツまで」　2022/01

  36. 顕微ラマン分光によるラベルフリー細胞生物研究 Invited

    中林孝和

    第35回日本放射光学会年会・放射光科学合同シンポジウム　2022/01

  37. 細胞内の水の測定から細胞内夾雑環境・液-液相分離を探る Invited

    中林孝和

    第30回日本バイオイメージング学会　2021/09

  38. 超硫黄分子の分子科学的アプローチ：量子化学計算・ラマン散乱 Invited

    中林孝和

    生理研研究会: 生命を支える硫黄生物学の最前線　2021/07

  39. ラマンイメージングを用いた細胞内の水の定量とラベルフリー温度計測への応用 Invited

    中林孝和

    Biothermology workshop　2020/12

  40. Water in a living cell: a Raman imaging approach Invited

    中林孝和

    令和2年度化学系学協会東北大会（物理化学コロキウム）　2020/09

  41. ラマンイメージングを用いた細胞内小分子のラベルフリー計測 Invited

    中林孝和

    第93回日本生化学会大会　2020/09

  42. Label-Free Evaluation of Intracellular Environmentsby Raman Imaging of Water International-presentation Invited

    NAKABAYASHI Takakazu

    13th International Symposium on Nanomedicine　2019/12

  43. Intracellular Water: New Parameters for Understanding Intracellular Environment International-presentation Invited

    NAKABAYASHI Takakazu

    Lecture of Department of Applied Chemistry at National Chiao Tung University　2019/11

  44. Raman imaging: application to detect water and biological molecules within a single cell International-presentation Invited

    NAKABAYASHI Takakazu

    1st International Conference on Persulfide and Sulfur Metabolism in Biology and Medicine　2019/09

  45. ラマンイメージングによる細胞内の水の観測－定量・密度分布・応用－ Invited

    NAKABAYASHI Takakazu

    蛋白研セミナー：細胞運命を決定する核空間制御　2019/08

  46. 細胞の中にある水のイメージング Invited

    NAKABAYASHI Takakazu

    第2回形態解析ワークショップ–多様な顕微鏡を用いて　2019/08

  47. Studies in Water in a Cell by Raman Microscopy: New Parameter for Evaluating Intracellular Environment International-presentation Invited

    NAKABAYASHI Takakazu

    12th International Symposium on Nanomedicine　2018/12

  48. Dynamic Response of Living Cells to Nanosecond Pulsed Electric Fields International-presentation Invited

    NAKABAYASHI Takakazu

    The 15th International Conference on Flow Dynamic　2018/11

  49. Label-Free Detection of Chemical Substances inside a Cell using Raman Imaging: Especially for the Detection of Water in a Cell Invited

    NAKABAYASHI Takakazu

    第41回日本分子生物学会年会　2018/11

  50. 細胞の中の水：分光計測と細胞内環境の理解 Invited

    NAKABAYASHI Takakazu

    第12回分子科学討論会　2018/09

  51. Molecular mechanism of the acquisition of toxicity of SOD1: metal depletion and intracellular environment Invited

    NAKABAYASHI Takakazu

    平成30年度化学系学協会東北大会　2018/09

  52. 分子分光学的手法の細胞内環境計測への応用 Invited

    NAKABAYASHI Takakazu

    第58回分子科学若手の会夏の学校　2018/08

  53. Mechanism of the pro-oxidant activity of wild-type SOD1 by the addition of structural instability International-presentation Invited

    NAKABAYASHI Takakazu

    43rd International Conference on Coordination Chemistry (ICCC2018)　2018/08

  54. 分子分光学的手法の細胞内環境計測への応用 Invited

    NAKABAYASHI Takakazu

    分離機能とセンシング機能の化学セミナー2018　2018/03

  55. Spectroscopic Techniques for Understanding Intracellular Environments: FLIM and Raman International-presentation Invited

    NAKABAYASHI Takakazu

    Interhierarchical understanding of materials and life through molecular observation　2018/03

  56. 細胞内環境を明らかにする分光学的手法の開発と薬学研究への展開 Invited

    NAKABAYASHI Takakazu

    日本薬学会東北支部第 39 回東北薬学セミナー　2017/12

  57. Direct Detection of Water inside a Living Cell using Raman Imaging Microscopy International-presentation Invited

    NAKABAYASHI Takakazu

    11th International Symposium on Nanomedicine　2017/12

  58. 分子分光学的手法の細胞計測への応用：ナノ秒パルス電場効果を中心に Invited

    NAKABAYASHI Takakazu

    仙台プラズマフォーラム　2017/11

  59. Spectroscopic and Photochemical Techniques for Understanding and Controlling Biological Systems International-presentation Invited

    NAKABAYASHI Takakazu

    Tohoku University's Chemistry Summer School 2017　2017/08

  60. Application of Spectroscopic and Photochemical Techniques to Nanomedicine International-presentation

    NAKABAYASHI Takakazu

    10th Anniversary International Symposium on Nanomedicine　2016/11

  61. 分子科学的手法を用いた細胞・タンパク質計測 Invited

    NAKABAYASHI Takakazu

    最先端光計測とライフサイエンスの近未来　2016/11

  62. Application of Molecular Spectroscopy to Biological Sciences International-presentation Invited

    NAKABAYASHI Takakazu

    Recent Progress in Molecular Spectroscopy and Dynamics　2016/07

  63. 光分子科学の手法を用いた細胞計測・制御の展開 Invited

    NAKABAYASHI Takakazu

    第38回日本光医学・光生物学会　2016/07

  64. Fluorescence Lifetime Imaging: Application to Intracellular Sensing International-presentation Invited

    NAKABAYASHI Takakazu

    2015 Tohoku University-Chungbuk National University-Sungkyunkwan University Joint Symposium on Pharmaceutical Sciences　2015/12

  65. 蛍光寿命イメージングによる細胞内環境の理解 Invited

    NAKABAYASHI Takakazu

    第10回東北大学学際科学フロンティア研究所FRISセミナー　2015/08

  66. 分子科学の細胞内への展開 Invited

    NAKABAYASHI Takakazu

    森野Discussion　2015/08

  67. 蛍光寿命イメージングによる細胞内環境測定 Invited

    NAKABAYASHI Takakazu

    新学術領域ナノメディシン分子科学公開シンポジウム　2015/07

  68. 蛍光寿命イメージングを用いた細胞計測：蛍光寿命変化の機構解明 Invited

    NAKABAYASHI Takakazu

    日本光学会年次学術講演会　2014/11

  69. 自家蛍光寿命を用いた生体分析化学の展開 Invited

    中林孝和, 太田信廣

    日本分析化学会第63年会　2014/09

  70. 蛍光寿命イメージングを用いた細胞観測の展開 Invited

    NAKABAYASHI Takakazu

    2013年度日本分光学会北海道支部シンポジウム　2014/01

  71. 分光技術を用いて細胞内の環境を探る–蛍光寿命イメージング– Invited

    NAKABAYASHI Takakazu

    ニコンイメージングセンター・ナノテクノロジープラットフォーム 学術講演会　2013/11

  72. 分光技術を用いて細胞内の環境を探る–蛍光寿命からのアプローチ– Invited

    NAKABAYASHI Takakazu

    分光イノベーション研究会第2回シンポジウム　2013/11

  73. 蛍光寿命イメージングを用いた細胞計測の展開 Invited

    中林 孝和

    日本分光学会平成２４年度生細胞分光部会シンポジウム　2013/01

  74. Application of Fluorescence Lifetime Imaging (FLIM) to Measure Intracellular Environments International-presentation Invited

    Takakazu Nakabayashi, Nobuhiro Ohta

    RIES-CIS Joint Symposium　2012/11

  75. 蛍光寿命の基礎と細胞観察への応用 Invited

    中林 孝和

    第18回細胞生物学ワークショップ　2011/11

  76. 蛍光寿命を用いた細胞のその場観察 Invited

    中林 孝和

    分子研シンポジウム2011　2011/05

  77. 蛍光寿命イメージングを用いた単一細胞計測の展開 Invited

    中林 孝和

    分子研研究会「分光学が係わるクラスター科学および機能性ナノ構造体科学 の将来展望」　2011/01

  78. 蛍光寿命の基礎と細胞観察に用いるメリット Invited

    中林 孝和

    第16回細胞生物学ワークショップ　2010/11

  79. Measurements of Intracellular Environments by Fluorescence Lifetime Imaging Microscopy International-presentation Invited

    T. Nakabayashi

    Symposium on Advanced Spectroscopy　2010/10

  80. 蛍光寿命イメージングを用いる細胞内環境計測の展開 Invited

    中林 孝和

    日本分光学会平成２１年度生細胞分光部会シンポジウム　2009/12

  81. 蛍光寿命の基礎と細胞観察への応用 Invited

    中林 孝和

    第14回細胞生物学ワークショップ　2009/11

  82. Studies on Microenvironment in a Single Cell using Fluorescence Lifetime Imaging Microscopy International-presentation Invited

    T. Nakabayashi

    Recent Advances in Fluorescence Spectroscopic Methods for Biological and Chemical Systems　2009/02

  83. 凝縮相の高速ダイナミクスの解明と細胞内現象への展開 Invited

    中林 孝和

    化学系学協会北海道支部2009年冬季研究発表会　2009/02

  84. Application of fluorescence lifetime imaging to the investigation of cellular microenvironments International-presentation Invited

    T. Nakabayashi, N. Ohta

    Annual Meeting of the Spectroscopical Society of Japan　2008/11

  85. ポリマー中における色素分子発光スペクトルへの電場効果と光励起ダイナミクス Invited

    中林 孝和

    有機エレクトロニクスに関する北大−名大合同研究会　2008/10

  86. In vivo measurements of intracellular environments by fluorescence lifetime imaging International-presentation Invited

    T. Nakabayashi, N. Ohta

    Joint Symposium on Dhaka University and Hokkaido University　2007/11

  87. Studies of Microenvironment in a Single Cell Using Fluorescence Lifetime Imaging Microscopy International-presentation Invited

    T. Nakabayashi

    The 12th Korea-Japan Joint Symposium on Frontiers of Molecular Science　2007/07

  88. 蛍光寿命イメージングを用いた細胞内の環境変化のIn Vivo計測 Invited

    中林 孝和

    分子研研究会「生細胞の分子科学」　2007/05

  89. 蛍光寿命イメージングを用いた細胞内の微視的環境変化の計測 Invited

    中林 孝和

    第68回分析化学討論会　2007/05

  90. Control of Emission Intensities of CdS Nanoparticles Using External Electric Fields International-presentation Invited

    T. Nakabayashi, N. Ohta

    International Symposium on Nanocluster Chemistry and Life Science　2007/01

  91. 蛍光寿命イメージングによる細胞内微視的環境の観測 Invited

    中林 孝和

    日本分光学会北海道支部シンポジウム「イメージング分光の生体および材料研究への応用」　2006/02

  92. 光イオン化に伴う芳香族イオンと取り囲む溶媒分子の超高速ダイナミクス Invited

    中林 孝和

    第22回ライラックセミナー・第11回若手研究者交流会　2005/06

  93. Ultrafast relaxation dynamics in liquid systems International-presentation Invited

    T. Nakabayashi

    The 6th RIES-Hokudai Symposium　2004/12

  94. ポリエン類の光励起ダイナミックスに対する外部電場効果 Invited

    中林 孝和

    分子研研究会「生体関連分子の水素結合とダイナミクスの新展開」　2003/07

  95. 時間分解ラマン分光法からみた溶液中の振動緩和過程の描像と他の超高速現象との関係 Invited

    中林 孝和

    分子研研究会「単純系から複雑系にわたる凝集系振動緩和ダイナミックス研究の現状と展望」　2003/06

  96. 水のクラスター構造―基本的な考え方と応用の可能性― Invited

    中林 孝和

    食品膜技術懇談会第14回春季研究例会　2002/06

  97. 芳香族イオンと取り囲む溶媒分子の超高速緩和過程 Invited

    中林 孝和

    「化学反応現象に特徴的な非平衡非定常性の解明―現代化学からみた統計理論の再考―」第一回公開シンポジウム　2002/03

  98. 分子クラスターの構造と物性：単一分子磁石を中心として Invited

    中林 孝和

    第１回新プログラム若手の会　2002/02

  99. 水の中のクラスター Invited

    中林 孝和, 西 信之

    日本食品科学工学会第48回大会　2001/09

  100. 液体の構造化学：回折法と振動分光法を用いた最近の研究とこれからの展開 Invited

    中林 孝和

    第13回液体の化学夏の学校　2001/08

  101. 水酸基を持つ液体の局所構造と分子レベルでの混合状態の経験則の提案 Invited

    中林 孝和

    分子科学から見た21世紀の溶液化学　2001/05

  102. Time-Resolved Raman and Absorption Studies of Photoionization of Aromatic Compounds in Polar Solvents: Ultrafast Relaxation Dynamics of Ions International-presentation Invited

    T. Nakabayashi

    Joint Seminar under the Japan-Korea Basic Scientific Cooperation Program　2001/01

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Industrial Property Rights 4

  1. 光誘起自家蛍光の時間分解測定による生物試料の低酸素領域分析方法とその装置

    Property Type: Patent

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    2010-053214

  2. 蛍光寿命を利用した細胞内のpHイメージング方法とその装置

    Property Type: Patent

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    2010-053215

  3. pH測定方法、異常領域検出方法、及び生体物質分析方法、並びに各装置

    Property Type: Patent

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    2010-053216

  4. 発光寿命測定装置およびその測定方法

    Property Type: Patent

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    2006-203816

Research Projects 31

  1. 液-液相分離を利用したタンパク質の高感度ラマンスペクトル測定手法の開発

    中林 孝和

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2024/06/28 - 2027/03/31

  2. 軟X線XAFS・ラマン同時測定による溶液試料の構造解析法開発と生体分子への展開

    佐伯 盛久, 中林 孝和, 藤井 健太郎, 中西 隆造

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 国立研究開発法人量子科学技術研究開発機構

    2023/04/01 - 2027/03/31

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    2024年度から稼働した放射光施設ナノテラスでは、高エネルギー分解能を備えた軟X線・テンダーX線ビームを発生できることから、生体分子の構造解析が可能なX線吸収分光装置の整備が求められている。生体分子では溶液中においてその機能を発現するものが多く、その反応機構を明らかにするには、溶液中の試料を「あるがままの状態」で構造解析する技術が必要である。X線吸収微細構造（XAFS）分光はこの目的に適した手法であるが、XAFS分光では化学結合に関与しない内殻電子励起を利用するため、結合長の微妙な変化には鈍感である。一方、同じく溶液中生体分子観察に利用されるラマン分光は、結合長の僅かな変化を反映してスペクトルが変化するので、反応に伴う微妙な構造変化を検出できる。そこで本課題ではXAFS分光とラマン分光の特長を相補的に利用したテンダーX線XAFS＋ラマン同時計測装置を製作し、特に複数の硫黄原子が連結した polysulfide-(S)n-を含む生体分子（超硫黄分子やSOD1タンパク質など）を対象とした構造解析法を開発し、ナノテラスで活用することを目指す。 S K吸収端（2.47 keV）付近でのXAFS測定は大気吸収による入射X線減衰を避けるため、通常真空環境下で行われるが、真空中での溶液試料の取り扱いは難しい。そこで令和5年度は、He大気圧環境下（2 keV以上のテンダーX線をほぼ透過）でのXAFS測定ができ、かつ溶液試料の取り扱いも容易な、He大気圧XAFS測定装置の設計を進めた。そして、①XAFS＋ラマン同時測定用の溶液試料セルを開発し、既存放射光施設での試験を進めるとともに、②多硫化物やSOD1タンパク質などで、適切な試料条件調整（濃度・サンプル量）により溶液試料のXAFSスペクトルを感度よく測定することに成功し、③独自のHe大気圧XAFS測定装置の設計も進めた。

  3. 軟X線XAFS・ラマン同時測定による溶液試料の構造解析法開発と生体分子への展開

    佐伯 盛久, 中林 孝和, 藤井 健太郎, 中西 隆造

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 国立研究開発法人量子科学技術研究開発機構

    2023/04/01 - 2027/03/31

  4. 非線形ラマン散乱を用いた細胞内超硫黄分子のin situ検出と動的変化の解明

    中林 孝和

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 学術変革領域研究(A)

    Institution: 東北大学

    2021/09/10 - 2026/03/31

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    (1) 分光器や検出器などの備品類を購入し、非線形ラマン散乱の一つであるCARS (Coherent anti-Stokes Raman Scattering) を用いた顕微鏡システムの立ち上げを行った。1秒以下の露光時間で高分子のC-H伸縮振動などに由来するCARSバンドの観測に成功した。次年度では、生細胞測定への拡張、またフィルター類を用いて350 cm-1までの低波数側の測定ができるように最適化する。(2) S-S伸縮振動バンド領域におけるシステインおよびグルタチオンの超硫黄分子のモデル化合物のラマンスペクトルが密度汎関数法を用いて再現し、量子化学計算によって構造解析が行えることを示した。得られた結果をもとに、顕微ラマン分光法を用いたタンパク質内に生成した超硫黄構造の検出を行った。リゾチームなどのタンパク質と硫化ナトリウムとの反応によって生じた超硫黄構造をS-S伸縮振動バンドの変化からその場検出した。現在、微量試料も測定できるように表面増強ラマン散乱(SERS)を用いた超硫黄構造の検出の検討を行っている。(3) ALS関連タンパク質SOD1はオリゴマー化することで毒性が増大することが提案されている。我々はS-S結合がSOD1の酸化毒性と関係することを示し、分子間のS-S結合を介して生成したオリゴマーが強い酸化毒性を生じることを示すことができた。オリゴマーの毒性の起源として酸化毒性を提案できる。また硫化ナトリウムをSOD1に作用させることで超硫黄化したSOD1の作成にも成功し、構造や酸化毒性との関係を検討している。

  5. Management of international relation and facility for promotion of research on sulfur biology

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Transformative Research Areas (A)

    Institution: Tohoku University

    2021/09/10 - 2026/03/31

  6. Spectroscopic analysis of phase-separated liquid droplets made from low-complexity sequences

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: National Institutes for Quantum Science and Technology

    2022/04/01 - 2025/03/31

  7. Spectroscopic analysis of phase-separated liquid droplets made from low-complexity sequences

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: National Institutes for Quantum Science and Technology

    2022/04/01 - 2025/03/31

  8. 液液相分離内のタンパク質の構造および分子間相互作用のラベルフリー直接計測

    中林 孝和

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2020/07/30 - 2023/03/31

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    液液相分離によって生じたタンパク質液滴について、紫外共鳴ラマン測定などのラマン分光を用いて分析する新規手法を提案することを目的とする。2021年度は、筋萎縮性側索硬化症関連タンパク質FUSおよびそのLC (Low-complexity) 領域 (FUS-LC)、Ataxin-2、さらにマシャドジョセフ病関連タンパク質Ataxin-3について、再現性の高い液滴生成法を確立し、紫外共鳴ラマン測定、水のラマンバンドを用いた強度標準について検討した。紫外共鳴ラマン測定では、硫酸ナトリウムを強度標準とした濃度測定プロトコルを確立し、液滴形成に伴うタンパク質のラマンバンド間の相対強度の変化、ピーク位置の変化について検討した。Ataxin-3を用いて測定を行い、トリプトファン (Trp) とチロシン (Tyr) の共鳴ラマンバンドのピーク位置に液滴形成に伴う変化は無かったが、これらの一部のバンドで強度増加が観測された。共鳴するTrpとTyrの吸収バンドの微小変化を観測していることが考えられる。FUSについても紫外共鳴ラマンスペクトルとTyrの自家蛍光寿命と組み合わせた検討を行っている。ラマン顕微鏡を用いた液滴内のタンパク質のラベルフリー濃度測定では、FUS-LCを用い、pH・塩濃度・試料調製時のFUS-LCの濃度、これらの依存性について系統的な測定を行いさらに液滴の溶解剤として用いられるヘキサンジオール (HD) の添加に伴う液滴内濃度変化の測定も行った。HDの添加に伴い液滴内のFUS-LCの濃度が減少し、液滴内のFUS-LCの濃度がある閾値以下になると液滴が消滅する液滴消滅機構を提案することができた。

  9. 超解像ラマンイメージングによる細胞内の水の観測と温度計測への応用 Competitive

    KAJIMOTO Shinji

    Offer Organization: 文部科学省

    System: 文部科学省 科学研究費 基盤研究B

    2019/04 - 2023/03

  10. 先駆水和電子チャネル形成仮説による水中プラズマ超高速電荷移動機構の学理構築 Competitive

    SATO Takehiko

    Offer Organization: 文部科学省

    System: 文部科学省 科学研究費 基盤研究A

    2019/04 - 2022/03

  11. 全反射ラマン顕微鏡を用いた生体膜近傍の水の選択的観測 Competitive

    NAKABAYASHI Takakazu

    Offer Organization: コーセーコスメトロジー研究財団

    System: コスメトロジー研究助成

    2019/11 - 2021/03

  12. 深紫外共鳴ラマン分光法を用いた細胞内分子のその場計測法の確立 Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 新学術領域領域 公募研究

    2017/04 - 2019/03

  13. ラマンイメージングによる生細胞内の水の定量 Competitive

    中林 孝和

    Offer Organization: 財団法人住友財団

    System: 基礎科学研究助成

    2017 - 2018

  14. 外部電場の印加によるタンパク質の動的変化の解明 Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 基盤研究B

    2014/04 - 2017/03

  15. 顕微ラマン分光によるがん細胞の電場アポトーシス誘起機構の解析 Competitive

    平松 弘嗣

    Offer Organization: 文部科学省

    System: 科学研究費 基盤研究C

    2015/04 - 2016/06

  16. 蛍光寿命イメージングを用いた細胞内イオン濃度の動的変化の解明 Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 新学術領域領域 公募研究

    2014/04 - 2016/03

  17. 三光子励起自家蛍光寿命イメージングを用いた細胞内環境変化のその場測 定 Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 新学術領域領域 公募研究

    2012/04 - 2014/03

  18. フェムト秒時間分解蛍光イメージングを用いた細胞内状態の三次元計測 Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 基盤研究B

    2010/04 - 2013/03

  19. 光と電場による反応制御と新奇外場応答機能物性の発現 Competitive

    太田 信廣

    Offer Organization: 文部科学省

    System: 科学研究費 基盤研究A

    2008/04 - 2012/03

  20. 非線形分子分光技術を用いた細胞イメージングシステムの開発 Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 萌芽研究

    2008/04 - 2010/03

  21. Development of Fluorescence Lifetime Imaging Microscopy to the Investigation of Cellular Microenvironments Competitive

    中林 孝和

    Offer Organization: 北海道大学

    System: 電子科学研究所プロジェクト研究(B)

    2008/04 - 2009/03

  22. 時間分解顕微吸収イメージングシステムによる非蛍光性の生体試料の画像観測 Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 若手研究A

    2006/04 - 2009/03

  23. 単一細胞内反応ダイナミクスにおけるシュタルク効果の計測 Competitive

    太田 信廣

    Offer Organization: 文部科学省

    System: 科学研究費 特定領域研究

    2004/04 - 2007/03

  24. 光、電場、磁場を摂動とする構造・反応・機能相関の研究 Competitive

    太田 信廣

    Offer Organization: 文部科学省

    System: 科学研究費 基盤研究A

    2003/04 - 2006/03

  25. 赤外シュタルク分光法を用いた巨大分子の構造−機能相関の解明 Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 若手研究A

    2003/04 - 2006/03

  26. 電磁場制御環境下における光化学反応の研究 Competitive

    太田 信廣

    Offer Organization: 文部科学省

    System: 科学研究費 特定領域研究

    2003/04 - 2005/03

  27. 超高速時間分解電場変調分光法の開発と電荷移動反応への応用 Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 萌芽研究

    2003/04 - 2005/03

  28. フェムト時間分解電場蛍光測定システムの開発と光機能性分子への応用 Competitive

    中林 孝和

    Offer Organization: 財団法人住友財団

    System: 基礎科学研究助成

    2004 - 2005

  29. ピコ秒誘導ラマン断熱透過法による溶液中のコヒーレント分布移動 Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 若手研究B

    2001/04 - 2003/03

  30. 水溶液中の特異なクラスター集合構造の発生と機能の発現 Competitive

    西 信之

    Offer Organization: 文部科学省

    System: 科学研究費 基盤研究B

    1999/04 - 2002/03

  31. 液体中に存在する中性およびイオン集合体の光解離ダイナミックス−時間分解 振動分光法による研究− Competitive

    中林 孝和

    Offer Organization: 文部科学省

    System: 科学研究費 奨励研究A

    1999/04 - 2001/03

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Social Activities 3

  1. 分子・スペクトルの外部電場効果：基礎から生体分子、細胞まで

    第58回分子科学若手の会夏の学校

    2018/08/20 - 2018/08/24

  2. 仙台西ロータリークラブ特別講演｢光を用いた最新の研究成果の紹介｣

    2015/11 - 2015/11

  3. 光のちからで生体を見る・測る・治療する ～光を用いた生命科学の最前線～

    2015/10 - 2015/10