Details of the Researcher

PHOTO

Hiroyuki Ishida
Section
Graduate School of Agricultural Science
Job title
Professor
Degree
  • 農学博士 (Tohoku University)

e-Rad No.
60312625

Research History 4

  • 2022/04 - Present
    Tohoku University Graduate School of Agricultural Science

  • 2009/04 - 2022/03
    東北大学大学院農学研究科 准教授

  • 2007/04 - 2009/03
    東北大学大学院農学研究科 助教

  • 1999/04 - 2007/03
    東北大学大学院農学研究科 助手

Education 2

  • Tohoku University Graduate School of Agricultural Science

    - 1999/03

  • 東北大学 農学部 農芸化学科

    - 1994/03

Committee Memberships 24

  • 財団法人翠生農学振興会助成金選考委員会 委員

    2014/04 - 2015/03

  • 財団法人翠生農学振興会募金委員会 委員

    2014/04 - 2015/03

  • 財団法人翠生農学振興会助成金選考委員会 委員

    2014/04 - 2015/03

  • 財団法人翠生農学振興会募金委員会 委員

    2014/04 - 2015/03

  • 財団法人翠生農学振興会助成金選考委員会 委員

    2013/04 - 2014/03

  • 財団法人翠生農学振興会募金委員会 委員

    2013/04 - 2014/03

  • 財団法人翠生農学振興会助成金選考委員会 委員

    2013/04 - 2014/03

  • 財団法人翠生農学振興会募金委員会 委員

    2013/04 - 2014/03

  • Saclay Plant Sciences 書面審査委員

    2017/03 -

  • Saclay Plant Sciences 書面審査委員

    2017/03 -

  • United States-Israel Binational Science Foundation 書面審査委員

    2016/03 -

  • Saclay Plant Sciences 書面審査委員

    2016/03 -

  • United States-Israel Binational Science Foundation 書面審査委員

    2016/03 -

  • Saclay Plant Sciences 書面審査委員

    2016/03 -

  • 米国植物生物学会 Plant Physiology 論文審査委員

    2015/10 -

  • 米国植物生物学会 Plant Physiology 論文審査委員

    2015/10 -

  • 米国植物生物学会 Plant Physiology 論文審査委員

    2015/03 -

  • 日本植物生理学会 Plant Cell Physiology 論文審査委員

    2015/03 -

  • 米国植物生物学会 Plant Physiology 論文審査委員

    2015/03 -

  • 日本植物生理学会 Plant Cell Physiology 論文審査委員

    2015/03 -

  • 米国植物生物学会 Plant Cell 論文審査委員

    2014/11 -

  • 米国植物生物学会 Plant Cell 論文審査委員

    2014/11 -

  • 日本植物生理学会 Plant Cell Physiology 論文審査委員

    2014/06 -

  • 日本植物生理学会 Plant Cell Physiology 論文審査委員

    2014/06 -

Show all ︎Show first 5

Professional Memberships 5

  • 米国植物生物学会

  • 日本光合成学会

  • 日本農芸化学会

  • 日本土壌肥料学会

  • 日本植物生理学会

Research Interests 7

  • 葉間窒素分配

  • イネ群落

  • Chloroplast/Plastid

  • Leaf senescence

  • Rubisco

  • Nitrogen remobilization

  • Autophagy

Research Areas 3

  • Environmental science/Agricultural science / Crop production science /

  • Life sciences / Applied molecular and cellular biology / Plant protein metabolism

  • Life sciences / Plant nutrition, soil science / Nitorgen use and remobilization in plants

Awards 1

  1. 第14回国際光合成会議ベストポスター賞

    2007/07 International Society of Photosynthesis Research 当該会議における全669題の発表のうち特に内容が優れていた7題に授与された

Papers 56

  1. Autophagy Contributes to the Quality Control of Leaf Mitochondria Peer-reviewed

    Sakuya Nakamura, Shinya Hagihara, Kohei Otomo, Hiroyuki Ishida, Jun Hidema, Tomomi Nemoto, Masanori Izumi

    Plant and Cell Physiology 2020/12/23

    Publisher: Oxford University Press (OUP)

    DOI: 10.1093/pcp/pcaa162  

    ISSN: 0032-0781

    eISSN: 1471-9053

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    <title>Abstract</title> In autophagy, cytoplasmic components of eukaryotic cells are transported to lysosomes or the vacuole for degradation. Autophagy is involved in plant tolerance to the photooxidative stress caused by ultraviolet B (UVB) radiation, but its roles in plant adaptation to UVB damage have not been fully elucidated. Here, we characterized organellar behavior in UVB-damaged Arabidopsis (Arabidopsis thaliana) leaves and observed the occurrence of autophagic elimination of dysfunctional mitochondria, a process termed mitophagy. Notably, Arabidopsis plants blocked in autophagy displayed increased leaf chlorosis after a 1-h UVB exposure compared to wild-type plants. We visualized autophagosomes by labeling with a fluorescent protein-tagged autophagosome marker, AUTOPHAGY8 (ATG8), and found that a 1-h UVB treatment led to increased formation of autophagosomes and the active transport of mitochondria into the central vacuole. In atg mutant plants, the mitochondrial population increased in UVB-damaged leaves due to the cytoplasmic accumulation of fragmented, depolarized mitochondria. Furthermore, we observed that autophagy was involved in the removal of depolarized mitochondria when mitochondrial function was disrupted by mutation of the FRIENDLY gene, which is required for proper mitochondrial distribution. Therefore, autophagy of mitochondria functions in response to mitochondrion-specific dysfunction as well as UVB damage. Together, these results indicate that autophagy is centrally involved in mitochondrial quality control in Arabidopsis leaves.

  2. Chloroplast Autophagy and Ubiquitination Combine to Manage Oxidative Damage and Starvation Responses. International-journal Peer-reviewed

    Yuta Kikuchi, Sakuya Nakamura, Jesse D Woodson, Hiroyuki Ishida, Qihua Ling, Jun Hidema, R Paul Jarvis, Shinya Hagihara, Masanori Izumi

    Plant Physiology 183 (4) 1531-1544 2020/08

    DOI: 10.1104/pp.20.00237  

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    Autophagy and the ubiquitin-proteasome system are the major degradation processes for intracellular components in eukaryotes. Although ubiquitination acts as a signal inducing organelle-targeting autophagy, the interaction between ubiquitination and autophagy in chloroplast turnover has not been addressed. In this study, we found that two chloroplast-associated E3 enzymes, SUPPRESSOR OF PPI1 LOCUS1 and PLANT U-BOX4 (PUB4), are not necessary for the induction of either piecemeal autophagy of chloroplast stroma or chlorophagy of whole damaged chloroplasts in Arabidopsis (Arabidopsis thaliana). Double mutations of an autophagy gene and PUB4 caused synergistic phenotypes relative to single mutations. The double mutants developed accelerated leaf chlorosis linked to the overaccumulation of reactive oxygen species during senescence and had reduced seed production. Biochemical detection of ubiquitinated proteins indicated that both autophagy and PUB4-associated ubiquitination contributed to protein degradation in the senescing leaves. Furthermore, the double mutants had enhanced susceptibility to carbon or nitrogen starvation relative to single mutants. Together, these results indicate that autophagy and chloroplast-associated E3s cooperate for protein turnover, management of reactive oxygen species accumulation, and adaptation to starvation.

  3. Transgenic rice overproducing Rubisco exhibits increased yields with improved nitrogen-use efficiency in an experimental paddy field Peer-reviewed

    Dong-Kyung Yoon, Keiki Ishiyama, Mao Suganami, Youshi Tazoe, Mari Watanabe, Serina Imaruoka, Maki Ogura, Hiroyuki Ishida, Yuji Suzuki, Mitsuhiro Obara, Tadahiko Mae, Amane Makino

    Nature Food 1 (2) 134-139 2020/02

    Publisher: Springer Science and Business Media LLC

    DOI: 10.1038/s43016-020-0033-x  

    eISSN: 2662-1355

  4. Vacuolar protein degradation via autophagy provides substrates to amino acid catabolic pathways as an adaptive response to sugar starvation in arabidopsis thaliana Invited Peer-reviewed

    Takaaki Hirota, Masanori Izumi, Masanori Izumi, Masanori Izumi, Shinya Wada, Shinya Wada, Amane Makino, Hiroyuki Ishida

    Plant and Cell Physiology 59 (7) 1363-1376 2018/07

    DOI: 10.1093/pcp/pcy005  

    ISSN: 0032-0781

  5. Selective elimination of membrane-damaged chloroplasts via microautophagy International-journal

    Sakuya Nakamura, Jun Hidema, Wataru Sakamoto, Hiroyuki Ishida, Masanori Izumi, Masanori Izumi, Masanori Izumi

    Plant Physiology 177 (3) 1007-1026 2018/06

    DOI: 10.1104/pp.18.00444  

    ISSN: 0032-0889

  6. Impacts of autophagy on nitrogen use efficiency in plants Invited Peer-reviewed

    Hiroyuki Ishida, Amane Makino

    Soil Science and Plant Nutrition 64 (1) 100-105 2018/01/02

    Publisher: Taylor and Francis Ltd.

    DOI: 10.1080/00380768.2017.1412239  

    ISSN: 1747-0765 0038-0768

  7. Entire Photodamaged Chloroplasts Are Transported to the Central Vacuole by Autophagy Peer-reviewed

    Masanori Izumi, Hiroyuki Ishida, Sakuya Nakamura, Jun Hidema

    PLANT CELL 29 (2) 377-394 2017/02

    DOI: 10.1105/tpc.16.00637  

    ISSN: 1040-4651

    eISSN: 1532-298X

  8. Autophagy is induced under Zn limitation and contributes to Zn-limited stress tolerance in Arabidopsis (Arabidopsis thaliana) Peer-reviewed

    Masatake Eguchi, Kazuhiko Kimura, Amane Makino, Hiroyuki Ishida

    SOIL SCIENCE AND PLANT NUTRITION 63 (4) 342-350 2017

    DOI: 10.1080/00380768.2017.1360750  

    ISSN: 0038-0768

    eISSN: 1747-0765

  9. Autophagy Supports Biomass Production and Nitrogen Use Efficiency at the Vegetative Stage in Rice Peer-reviewed

    Shinya Wada, Yasukazu Hayashida, Masanori Izumi, Takamitsu Kurusu, Shigeru Hanamata, Keiichi Kanno, Soichi Kojima, Tomoyuki Yamaya, Kazuyuki Kuchitsu, Amane Makino, Hiroyuki Ishida

    PLANT PHYSIOLOGY 168 (1) 60-U721 2015/05

    DOI: 10.1104/pp.15.00242  

    ISSN: 0032-0889

    eISSN: 1532-2548

  10. Establishment of Monitoring Methods for Autophagy in Rice Reveals Autophagic Recycling of Chloroplasts and Root Plastids during Energy Limitation

    Masanori Izumi, Jun Hidema, Shinya Wada, Eri Kondo, Takamitsu Kurusu, Kazuyuki Kuchitsu, Amane Makino, Hiroyuki Ishida

    PLANT PHYSIOLOGY 167 (4) 1307-U316 2015/04

    DOI: 10.1104/pp.114.254078  

    ISSN: 0032-0889

    eISSN: 1532-2548

  11. OsATG7 is required for autophagy-dependent lipid metabolism in rice postmeiotic anther development

    Takamitsu Kurusu, Tomoko Koyano, Shigeru Hanamata, Takahiko Kubo, Yuhei Noguchi, Chikako Yagi, Noriko Nagata, Takashi Yamamoto, Takayuki Ohnishi, Yozo Okazaki, Nobutaka Kitahata, Daichi Ando, Masaya Ishikawa, Shinya Wada, Akio Miyao, Hirohiko Hirochika, Hiroaki Shimada, Amane Makino, Kazuki Saito, Hiroyuki Ishida, Tetsu Kinoshita, Nori Kurata, Kazuyuki Kuchitsu

    AUTOPHAGY 10 (5) 878-888 2014/05

    DOI: 10.4161/auto.28279  

    ISSN: 1554-8627

    eISSN: 1554-8635

  12. Roles of autophagy in chloroplast recycling Invited Peer-reviewed

    Hiroyuki Ishida, Masanori Izumi, Shinya Wada, Amane Makino

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1837 (4) 512-521 2014/04

    DOI: 10.1016/j.bbabio.2013.11.009  

    ISSN: 0005-2728

    eISSN: 0006-3002

  13. Evidence for contribution of autophagy to Rubisco degradation during leaf senescence in Arabidopsis thaliana

    Yuki Ono, Shinya Wada, Masanori Izumi, Amane Makino, Hiroyuki Ishida

    PLANT CELL AND ENVIRONMENT 36 (6) 1147-1159 2013/06

    DOI: 10.1111/pce.12049  

    ISSN: 0140-7791

  14. Autophagy Contributes to Nighttime Energy Availability for Growth in Arabidopsis Peer-reviewed

    Masanori Izumi, Jun Hidema, Amane Makino, Hiroyuki Ishida

    PLANT PHYSIOLOGY 161 (4) 1682-1693 2013/04

    DOI: 10.1104/pp.113.215632  

    ISSN: 0032-0889

  15. A possible involvement of autophagy in amyloplast degradation in columella cells during hydrotropic response of Arabidopsis roots Peer-reviewed

    Mayumi Nakayama, Yasuko Kaneko, Yutaka Miyazawa, Nobuharu Fujii, Nahoko Higashitani, Shinya Wada, Hiroyuki Ishida, Kohki Yoshimoto, Ken Shirasu, Kenji Yamada, Mikio Nishimura, Hideyuki Takahashi

    PLANTA 236 (4) 999-1012 2012/10

    DOI: 10.1007/s00425-012-1655-5  

    ISSN: 0032-0935

  16. RBCS1A and RBCS3B, two major members within the Arabidopsis RBCS multigene family, function to yield sufficient Rubisco content for leaf photosynthetic capacity Peer-reviewed

    Masanori Izumi, Honami Tsunoda, Yuji Suzuki, Amane Makino, Hiroyuki Ishida

    JOURNAL OF EXPERIMENTAL BOTANY 63 (5) 2159-2170 2012/03

    DOI: 10.1093/jxb/err434  

    ISSN: 0022-0957

  17. The Autophagic Degradation of Chloroplasts via Rubisco-Containing Bodies Is Specifically Linked to Leaf Carbon Status But Not Nitrogen Status in Arabidopsis Peer-reviewed

    Masanori Izumi, Shinya Wada, Amane Makino, Hiroyuki Ishida

    PLANT PHYSIOLOGY 154 (3) 1196-1209 2010/11

    DOI: 10.1104/pp.110.158519  

    ISSN: 0032-0889

  18. Autophagy Plays a Role in Chloroplast Degradation during Senescence in Individually Darkened Leaves Peer-reviewed

    Shinya Wada, Hiroyuki Ishida, Masanori Izumi, Kohki Yoshimoto, Yoshinori Ohsumi, Tadahiko Mae, Amane Makino

    PLANT PHYSIOLOGY 149 (2) 885-893 2009/02

    DOI: 10.1104/pp.108.130013  

    ISSN: 0032-0889

    eISSN: 1532-2548

  19. Mobilization of rubisco and stroma-localized fluorescent proteins of chloroplasts to the vacuole by an ATG gene-dependent autophagic process Peer-reviewed

    Hiroyuki Ishida, Kohki Yoshimoto, Masanori Izumi, Daniel Reisen, Yuichi Yano, Amane Makino, Yoshinori Ohsumi, Maureen R. Hanson, Tadahiko Mae

    PLANT PHYSIOLOGY 148 (1) 142-155 2008/09

    DOI: 10.1104/pp.108.122770  

    ISSN: 0032-0889

  20. Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts

    Masanori Izumi, Sakuya Nakamura, Kohei Otomo, Hiroyuki Ishida, Jun Hidema, Tomomi Nemoto, Shinya Hagihara

    eLife 2024/11/07

    DOI: 10.7554/eLife.93232  

  21. The gs3 allele from a large‐grain rice cultivar, Akita 63, increases yield and improves nitrogen‐use efficiency

    Dong‐Kyung Yoon, Mao Suganami, Keiki Ishiyama, Takaaki Kagawa, Marin Tanaka, Rina Nagao, Daisuke Takagi, Hiroyuki Ishida, Yuji Suzuki, Tadahiko Mae, Amane Makino, Mitsuhiro Obara

    Plant Direct 6 (7) 2022/07

    Publisher: Wiley

    DOI: 10.1002/pld3.417  

    ISSN: 2475-4455

    eISSN: 2475-4455

  22. Photosynthetic Enhancement, Lifespan Extension, and Leaf Area Enlargement in Flag Leaves Increased the Yield of Transgenic Rice Plants Overproducing Rubisco Under Sufficient N Fertilization. International-journal

    Marin Tanaka, Mamoru Keira, Dong-Kyung Yoon, Tadahiko Mae, Hiroyuki Ishida, Amane Makino, Keiki Ishiyama

    Rice (New York, N.Y.) 15 (1) 10-10 2022/02/09

    DOI: 10.1186/s12284-022-00557-5  

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    BACKGROUND: Improvement in photosynthesis is one of the most promising approaches to increase grain yields. Transgenic rice plants overproducing Rubisco by 30% (RBCS-sense rice plants) showed up to 28% increase in grain yields under sufficient nitrogen (N) fertilization using an isolated experimental paddy field (Yoon et al. in Nat Food 1:134-139, 2020). The plant N contents above-ground sections and Rubisco contents of the flag leaves were higher in the RBCS-sense plants than in the wild-type rice plants during the ripening period, which may be reasons for the increased yields. However, some imprecise points were left in the previous research, such as contributions of photosynthesis of leaves below the flag leaves to the yield, and maintenance duration of high photosynthesis of RBCS-sense rice plants during ripening periods. RESULT: In this research, the photosynthetic capacity and canopy architecture were analyzed to explore factors for the increased yields of RBCS-sense rice plants. It was found that N had already been preferentially distributed into the flag leaves at the early ripening stage, contributing to maintaining higher Rubisco content levels in the enlarged flag leaves and extending the lifespan of the flag leaves of RBCS-sense rice plants throughout ripening periods under sufficient N fertilization. The higher amounts of Rubisco also improved the photosynthetic activity in the flag leaves throughout the ripening period. Although the enlarged flag leaves of the RBCS-sense rice plants occupied large spatial areas of the uppermost layer in the canopy, no significant prevention of light penetration to leaves below the flag leaves was observed. Additionally, since the CO2 assimilation rates of lower leaves between wild-type and RBCS-sense rice plants were the same at the early ripening stage, the lower leaves did not contribute to an increase in yields of the RBCS-sense rice plants. CONCLUSION: We concluded that improvements in the photosynthetic capacity by higher leaf N and Rubisco contents, enlarged leaf area and extended lifespan of flag leaves led to an increase in grain yields of RBCS-sense rice plants grown under sufficient N fertilization.

  23. Flavodiiron protein rescues defects in electron transport around PSI due to overproduction of Rubisco activase in rice. International-journal

    Mao Suganami, So Konno, Ryo Maruhashi, Daisuke Takagi, Youshi Tazoe, Shinya Wada, Hiroshi Yamamoto, Toshiharu Shikanai, Hiroyuki Ishida, Yuji Suzuki, Amane Makino

    Journal of experimental botany 73 (8) 2589-2600 2022/02/02

    DOI: 10.1093/jxb/erac035  

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    The fragility of photosystem I (PSI) was observed in transgenic rice plants overproducing Rubisco activase (RCA) (RCA-ox). This study examined the effects of RCA overproduction on PSI photoinhibition sensitivity in three lines of RCA-ox plants. The quantum yield of PSI [Y(I)] decreased, and the quantum yield of acceptor-side limitation of PSI [Y(NA)] conversely increased in all lines of RCA-ox plants, especially under low light conditions. In RCA-ox plants with the highest RCA content (RCA-ox 1), the quantum yield of PSII [Y(II)] and CO2 assimilation also decreased under low light. When leaves were exposed to high light (2,000 μmol photon m -2 s -1) for 60 min, the maximal activity of PSI (Pm) drastically decreased in RCA-ox 1. These results suggested that RCA overproduction disturbs PSI electron transport control, increasing PSI photoinhibition susceptibility. When flavodiiron protein (FLV), which functions as a large electron sink downstream of PSI, was introduced into RCA-ox 1 (RCA-FLV), PSI, PSII parameters, and CO2 assimilation in RCA-FLV plants were recovered to wild-type plant levels. Thus, FLV introduction restored PSI robustness in RCA-ox plants.

  24. GFS9 affects piecemeal autophagy of plastids in young seedlings of Arabidopsis thaliana.

    Hiroyuki Ishida, Yu Okashita, Hiromi Ishida, Makoto Hayashi, Masanori Izumi, Amane Makino, Nazmul H Bhuiyan, Klaas J Van Wijk

    Plant and Cell Physiology 2021/06/04

    DOI: 10.1093/pcp/pcab084  

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    Chloroplasts, and plastids in general, contain abundant protein pools that can be major sources of carbon and nitrogen for recycling. We have previously shown that chloroplasts are partially and sequentially degraded by piecemeal autophagy via the Rubisco-containing body (RCB). This degradation occurs during plant development and in response to the environment; however, little is known about the fundamental underlying mechanisms. To discover the mechanisms of piecemeal autophagy of chloroplasts/plastids, we conducted a forward-genetics screen following ethyl-methanesulfonate (EMS) mutagenesis of an Arabidopsis (Arabidopsis thaliana) transgenic line expressing chloroplast-targeted GFP. This screen allowed us to isolate a mutant, gfs9-5, which hyperaccumulated cytoplasmic bodies labeled with chloroplast-targeted GFP of up to 1.0 μm in diameter in the young seedlings. We termed these structures plastid bodies (PBs). The mutant was defective in a membrane-trafficking factor, GREEN FLUORESCENT SEED 9 (GFS9), and PB accumulation in gfs9-5 was promoted by darkness and nutrient deficiency. Transmission electron microscopy indicated that gfs9-5 hyperaccumulated structures corresponding to autophagosomes and PBs. gfs9-5 hyperaccumulated membrane-bound endogenous ATG8 proteins, transgenic YFP-ATG8e proteins, and autophagosome-like structures labeled with YFP-ATG8e. The YFP-ATG8e signal was associated with the surface of plastids and their protrusions in gfs9-5. Double mutants of gfs9 and autophagy-defective 5 (atg5) did not accumulate PBs. In gfs9-5, the YFP-ATG8e proteins and PBs could be delivered to the vacuole and autophagic flux was increased. We discuss a possible connection between GFS9 and autophagy and propose a potential use of gfs9-5 as a new tool to study piecemeal plastid autophagy.

  25. An additional role for chloroplast proteins-an amino acid reservoir for energy production during sugar starvation Peer-reviewed

    Masanori Izumi, Hiroyuki Ishida

    Plant Signaling and Behavior 14 (1) 1552057-1552057 2019/01

    Publisher: Informa {UK} Limited

    DOI: 10.1080/15592324.2018.1552057  

    ISSN: 1559-2324

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    本人がCorresponding author(*)である。<br /> IF=1.395

  26. Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

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    Autophagy 12 (2) 443-443 2016

    DOI: 10.1080/15548627.2016.1147886  

    ISSN: 1554-8635 1554-8627

  27. From Arabidopsis to cereal crops: Conservation of chloroplast protein degradation by autophagy indicates its fundamental role in plant productivity Peer-reviewed

    Masanori Izumi, Jun Hidema, Hiroyuki Ishida

    PLANT SIGNALING & BEHAVIOR 10 (11) e1101199 2015/11

    DOI: 10.1080/15592324.2015.1101199  

    ISSN: 1559-2316

    eISSN: 1559-2324

  28. Deficiency of autophagy leads to significant changes of metabolic profiles in Arabidopsis Peer-reviewed

    Masanori Izumi, Jun Hidema, Hiroyuki Ishida

    Plant Signaling and Behavior 8 (8) 20130000 2013/08

    DOI: 10.4161/psb.25023  

    ISSN: 1559-2316 1559-2324

  29. Crystal Structure of Rice Rubisco and Implications for Activation Induced by Positive Effectors NADPH and 6-Phosphogluconate

    Hiroyoshi Matsumura, Eiichi Mizohata, Hiroyuki Ishida, Ayako Kogami, Takeshi Ueno, Amane Makino, Tsuyoshi Inoue, Akiho Yokota, Tadahiko Mae, Yasushi Kai

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    DOI: 10.1016/j.jmb.2012.05.014  

    ISSN: 0022-2836

  30. Guidelines for the use and interpretation of assays for monitoring autophagy

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Zakeri, Z., Zeh III, H.J., Zeitlin, S.O., Zhang, H., Zhang, H.-L., Zhang, J., Zhang, J.-P., Zhang, L., Zhang, L., Zhang, M.-Y., Zhang, X.D., Zhao, M., Zhao, Y.-F., Zhao, Y., Zhao, Z.J., Zheng, X., Zhivotovsky, B., Zhong, Q., Zhou, C.-Z., Zhu, C., and Zhu, W.-G., and Zhu, X.-F., Zhu, X., Zhu, Y., Zoladek, T., Zong, W.-X., Zorzano, A., Zschocke, J., Zuckerbraun, B.

    Autophagy 8 (4) 2012

    DOI: 10.4161/auto.19496  

    ISSN: 1554-8627 1554-8635

  31. The changes of leaf carbohydrate contents as a regulator of autophagic degradation of chloroplasts via rubisco-containing bodies during leaf senescence Invited Peer-reviewed

    Masanori Izumi, Hiroyuki Ishida

    Plant Signaling and Behavior 6 (5) 685-687 2011/05

    DOI: 10.4161/psb.6.5.14949  

    ISSN: 1559-2316 1559-2324

  32. Cyclobutane pyrimidine dimer (CPD) photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA Peer-reviewed

    Masaaki Takahashi, Mika Teranishi, Hiroyuki Ishida, Junji Kawasaki, Atsuko Takeuchi, Tomoyuki Yamaya, Masao Watanabe, Amane Makino, Jun Hidema

    PLANT JOURNAL 66 (3) 433-442 2011/05

    DOI: 10.1111/j.1365-313X.2011.04500.x  

    ISSN: 0960-7412

  33. Rubisco turnover and nitrogen in a leaf Invited

    Ishida, H, Suzuki, Y, Makino A

    Nitorgen assimilation in plants 277-285 2010

  34. Protein Turnover in Grass Leaves Invited Peer-reviewed

    Louis John Irving, Yuji Suzuki, Hiroyuki Ishida, Amane Makino

    ADVANCES IN BOTANICAL RESEARCH, VOL 54 54 139-182 2010

    DOI: 10.1016/S0065-2296(10)54004-7  

    ISSN: 0065-2296

  35. Biochemical changes associated with in vivo RbcL fragmentation by reactive oxygen species under chilling-light conditions Peer-reviewed

    R. Nakano, H. Ishida, M. Kobayashi, A. Makino, T. Mae

    PLANT BIOLOGY 12 (1) 35-45 2010/01

    DOI: 10.1111/j.1438-8677.2009.00209.x  

    ISSN: 1435-8603

    eISSN: 1438-8677

  36. The role of autophagy in nutrient starvation and aging Invited

    Yoshimoto K, Ishida H, Wada S, Ohsumi Y, Shirasu K

    Autophagy 2009/09

  37. The analysis of factors affecting the creation of Rubisco-containing bodies(RCBs), a specific autophagic process for chloroplast degradation in plants

    Izumi M, Ishida H, Makino A

    Autophagy 2009/09

  38. The chloroplast degradation in two different pathways in individually darkened leaves of Arabidopsis by autophagy

    Wada S, Ishida H, Yoshimoto K, Ohsumi Y, Makino A

    Autophagy 2009/09

  39. Autophagy of whole and partial chloroplasts in individually darkened leaves A unique system in plants? Invited Peer-reviewed

    Hiroyuki Ishida, Shinya Wada

    AUTOPHAGY 5 (5) 736-737 2009/07

    DOI: 10.4161/auto.5.5.8568  

    ISSN: 1554-8627

  40. Chloroplasts autophagy during senescence of individually darkened leaves Invited Peer-reviewed

    Shinya Wada, Hiroyuki Ishida

    Plant Signaling and Behavior 4 (6) 565-567 2009/06

    DOI: 10.4161/psb.4.6.8877  

    ISSN: 1559-2316 1559-2324

  41. Chloroplasts are partially mobilized to the vacuole by autophagy Invited Peer-reviewed

    Hiroyuki Ishida, Kohki Yoshimoto

    AUTOPHAGY 4 (7) 961-962 2008/10

    DOI: 10.4161/auto.6804  

    ISSN: 1554-8627

    eISSN: 1554-8635

  42. Visualization of Rubisco-containing bodies derived from chloroplasts in living cells of Arabidopsis. Peer-reviewed

    Ishida, H, Yoshimoto, K, Reisen, D, Makino, A, Ohsumi, Y, Hanson, M.R, Mae, T

    Photosynthesis. Energy from the sun 1207-1210 2008/09

  43. Rubisco and photosynthesis in cereal crops Invited

    Makino, A, Ishida, H

    Tohoku Journal of Agricultural Research 58 127-135 2008

  44. Upregulation of a tonoplast-localized cytochrome P450 during petal senescence in Petunia inflata Peer-reviewed

    Yan Xu, Hiroyuki Ishida, Daniel Reisen, Maureen R. Hanson

    BMC PLANT BIOLOGY 6 (6) 8 2006/04

    DOI: 10.1186/1471-2229-6-8  

    ISSN: 1471-2229

  45. In vivo fragmentation of the large subunit of ribulose-1,5-bisphosphate carboxylase by reactive oxygen species in an intact leaf of cucumber under chilling-light conditions Peer-reviewed

    R Nakano, H Ishida, A Makino, T Mae

    PLANT AND CELL PHYSIOLOGY 47 (2) 270-276 2006/02

    DOI: 10.1093/pcp/pci245  

    ISSN: 0032-0781

  46. Ribulose-1,5-bisphosphate carboxylase/oxygenase is excluded from chloroplasts by specific bodies in senescing wheat and rice leaves

    Ishida H, Chiba A, Hikichi R, Nishizawa N.K, Makino A, Mae T

    Photosynthesis: Fundamental Aspects to Global Perspectives (2) 837-838 2005

  47. Fragmentation of the large subunit of ribulose-1,5-bisphosphate carboxylase by reactive oxygen species occurs in vivo

    Nakano R, Ishida H, Makino A, Mae T

    Photosynthesis: Fundamental Aspects to Global Perspectives (1) 509-510 2005

  48. Exclusion of ribulose-1,5-bisphosphate carboxylase/oxygenase from chloroplasts by specific bodies in naturally senescing leaves of wheat Peer-reviewed

    A Chiba, H Ishida, NK Nishizawa, A Makino, T Mae

    PLANT AND CELL PHYSIOLOGY 44 (9) 914-921 2003/09

    DOI: 10.1093/pcp/pcg118  

    ISSN: 0032-0781

  49. The degradation of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase into the 44-kDa fragment in the lysates of chloroplasts incubated in darkness Peer-reviewed

    N Kokubun, H Ishida, A Makino, T Mae

    PLANT AND CELL PHYSIOLOGY 43 (11) 1390-1395 2002/11

    DOI: 10.1093/pcp/pcf159  

    ISSN: 0032-0781

  50. Direct evidence for non-enzymatic fragmentation of chloroplastic glutamine synthetase by a reactive oxygen species Peer-reviewed

    H Ishida, D Anzawa, N Kokubun, A Makino, T Mae

    PLANT CELL AND ENVIRONMENT 25 (5) 625-631 2002/05

    DOI: 10.1046/j.1365-3040.2002.00851.x  

    ISSN: 0140-7791

  51. Fe2+-catalyzed site-specific cleavage of the large subunit of ribulose 1,5-bisphosphate carboxylase close to the active site Peer-reviewed

    S Luo, H Ishida, A Makino, T Mae

    JOURNAL OF BIOLOGICAL CHEMISTRY 277 (14) 12382-12387 2002/04

    DOI: 10.1074/jbc.M111072200  

    ISSN: 0021-9258

  52. Fragmentation of the large subunit of ribulose-1,5-bisphosphate carboxylase by reactive oxygen species occurs near Gly-329 Peer-reviewed

    H Ishida, A Makino, T Mae

    JOURNAL OF BIOLOGICAL CHEMISTRY 274 (8) 5222-5226 1999/02

    DOI: 10.1074/jbc.274.8.5222  

    ISSN: 0021-9258

  53. Light-dependent fragmentation of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase in chloroplasts isolated from wheat leaves Peer-reviewed

    H Ishida, S Shimizu, A Makino, T Mae

    PLANTA 204 (3) 305-309 1998/03

    DOI: 10.1007/s004250050260  

    ISSN: 0032-0935

  54. Determination of the cleavage site of the large subunit of rubisco by active oxygen species and its inhibition by CABP in the lysates of wheat chloroplasts

    H Ishida, A Makino, T Mae

    PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V (3) 2245-2248 1998

  55. Light-dependent fragmentation of ribulose-1,5-bisphosphate carboxylase/oxygenase in chloroplasts isolated from wheat leaves

    H Ishida, S Shimizu, A Makino, T Mae

    PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V (3) 2249-2252 1998

  56. The large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is fragmented into 37-kDa and 16-kDa polypeptides by active oxygen in the lysates of chloroplasts from primary leaves of wheat Peer-reviewed

    H Ishida, Y Nishimori, M Sugisawa, A Makino, T Mae

    PLANT AND CELL PHYSIOLOGY 38 (4) 471-479 1997/04

    ISSN: 0032-0781

Show all ︎Show first 5

Misc. 64

  1. 4-1-21 大粒多収イネ、秋田63号の大粒有用形質を持つ準同質遺伝子系統イネの収量評価試験(4-1 植物の多量栄養素 2021年度北海道大会)

    尹 棟敬, 石山 敬貴, 菅波 真央, 田中 まりん, 永尾 梨奈, 鈴木 雄二, 石田 宏幸, 前 忠彦, 牧野 周, 小原 実広

    日本土壌肥料学会講演要旨集 67 50-50 2021/09/03

    Publisher: 一般社団法人 日本土壌肥料学会

    DOI: 10.20710/dohikouen.67.0_50_3  

    ISSN: 0288-5840

    eISSN: 2424-0575

  2. 4-1-18 欠損GS3 遺伝子により大粒性を有した準同質遺伝子系統ノトヒカリの作出とほ場での収量評価試験(4-1 植物の多量栄養素 2020年度岡山大会)

    尹 棟敬, 香川 昂亮, 石山 敬貴, 菅波 真央, 前 忠彦, 鈴木 雄二, 石田 宏幸, 牧野 周, 小原 実広

    日本土壌肥料学会講演要旨集 66 42-42 2020/09/01

    Publisher: 一般社団法人 日本土壌肥料学会

    DOI: 10.20710/dohikouen.66.0_42_3  

    ISSN: 0288-5840

    eISSN: 2424-0575

  3. 4-1-31 遺伝子組換え植物専用隔離ほ場水田を用いたRubisco増強イネの収量評価試験(4-1 植物の多量栄養素 2020年度岡山大会)

    尹 棟敬, 香川 昂亮, 石山 敬貴, 菅波 眞央, 前 忠彦, 鈴木 雄二, 石田 宏幸, 小原 実広, 牧野 周

    日本土壌肥料学会講演要旨集 66 47-47 2020/09/01

    Publisher: 一般社団法人 日本土壌肥料学会

    DOI: 10.20710/dohikouen.66.0_47_1  

    ISSN: 0288-5840

    eISSN: 2424-0575

  4. 4-1-4 遺伝子組換え隔離ほ場におけるRubisco過剰生産イネのバイオマス生産、窒素利用および収量解析(4-1 植物の多量栄養素,2019年静岡大会)

    尹 棟敬, 石山 敬貴, 菅波 眞央, 香川 昂亮, 渡邊 まり, 伊丸岡 芹菜, 小倉 真紀, 田副 雄士, 石田 宏幸, 鈴木 雄二, 小原 実広, 前 忠彦, 牧野 周

    日本土壌肥料学会講演要旨集 65 42-42 2019/09/03

    Publisher: 一般社団法人 日本土壌肥料学会

    DOI: 10.20710/dohikouen.65.0_42_1  

    ISSN: 0288-5840

    eISSN: 2424-0575

  5. 4-1-15 異なる光環境におけるイネの栄養成長とオートファジー(4-1 植物の多量栄養素,2016年度佐賀大会)

    和田 慎也, 山内 雄太, 石田 宏幸, 牧野 周

    日本土壌肥料学会講演要旨集 62 47-47 2016

    Publisher: 一般社団法人 日本土壌肥料学会

    DOI: 10.20710/dohikouen.62.0_47_3  

  6. P4-1-12 イネの栄養成長と老化葉の窒素リサイクルにおけるオートファジーの役割の解析(ポスター,4-1 植物の多量栄養素,2015年度京都大会)

    和田 慎也, 林田 泰和, 泉 正範, 来須 孝光, 花俣 繁, 朽津 和幸, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (61) 62-62 2015/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  7. 4-1-5 イネの発芽過程におけるオートファジーの役割(4-1 植物の多量栄養素,2015年度京都大会)

    和田 慎也, 中村 萌, 石田 宏幸, 牧野 周

    日本土壌肥料学会講演要旨集 (61) 49-49 2015/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  8. 4-1-6 シロイヌナズナのRCBおよび葉緑体オートファジーにおけるATI(ATG8-interacting proteins)の役割について(4-1 植物の多量栄養素,2015年度京都大会)

    石田 宏幸, 西村 翼, 泉 正範, Galili Gad, 牧野 周

    日本土壌肥料学会講演要旨集 (61) 49-49 2015/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  9. 9 糖欠乏下における一時的なエネルギー供給系としてのオートファジーの役割(東北支部講演会,2013年度各支部会)

    泉 正範, 弘田 隆晃, 日出間 純, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (60) 254-254 2014/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  10. 4-1-3 シロイヌナズナにおける糖欠乏下でのオートファジーの役割の解析(4-1 植物の多量栄養素,2014年度東京大会)

    弘田 隆晃, 泉 正範, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (60) 47-47 2014/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  11. 4-1-5 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に及ぼす影響の解析(4-1 植物の多量栄養素,2014年度東京大会)

    和田 慎也, 横浜 諒, 石田 宏幸, 牧野 周

    日本土壌肥料学会講演要旨集 (60) 48-48 2014/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  12. 4-1-4 シロイヌナズナにおける葉緑体オートファジーの機能解析 : 光障害時の役割について(4-1 植物の多量栄養素,2014年度東京大会)

    泉 正範, 石田 宏幸, 牧野 周, 日出間 純

    日本土壌肥料学会講演要旨集 (60) 48-48 2014/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  13. 4-2-1 シロイヌナズナにおけるオートファジーの欠損が各栄養素の欠乏時の生存と成長に及ぼす影響の解析(4-2 植物の微量栄養素,2014年度東京大会)

    江口 雅丈, 泉 正範, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (60) 61-61 2014/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  14. 植物の栄養リサイクルと葉緑体のオートファジー

    石田 宏幸

    化学と生物 52 610-615 2014/09

    DOI: 10.1271/kagakutoseibutsu.52.610  

  15. 5 シロイヌナズナにおけるオートファジーによる葉緑体分解と栄養素のリサイクル(東北支部講演会,2012年度各支部会)

    江口 雅丈, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (59) 252-252 2013/09/11

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  16. 8 オートファジー関連遺伝子ATG7の欠損がイネのバイオマス及び窒素利用に及ぼす影響の解析(東北支部講演会,2012年度各支部会)

    和田 慎也, 林田 泰和, 来須 孝光, 朽津 和幸, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (59) 253-253 2013/09/11

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  17. 4-1-17 イネにおけるRubisco-containing body/オートファジー系の可視化(4-1 植物の多量栄養素)

    泉 正範, 日出間 純, 近藤 依里, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (59) 59-59 2013/09/11

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  18. 4-1-16 イネオートファジー欠損変異体Osatg7の生理解析 : (その2)葉の老化過程における窒素転流への影響について(4-1 植物の多量栄養素)

    林田 泰和, 和田 慎也, 来須 孝光, 朽津 和幸, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (59) 59-59 2013/09/11

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  19. 4-1-15 イネオートファジー欠損変異体Osatg7の生理解析 : (その1)栄養成長期における個体生育について(4-1 植物の多量栄養素)

    和田 慎也, 林田 泰和, 来須 孝光, 朽津 和幸, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (59) 58-58 2013/09/11

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  20. Deficiency of autophagy leads to significant changes of metabolic profiles in Arabidopsis

    Masanori Izumi, Jun Hidema, Hiroyuki Ishida

    Plant Signaling and Behavior 8 (8) 2013/08

    DOI: 10.4161/psb.25023  

    ISSN: 1559-2316 1559-2324

  21. 9-5 シロイヌナズナ葉の栄養素リサイクルにおいてRCB/オートファジー系が担う役割の解析(9.植物の多量栄養素,2012年度鳥取大会)

    泉 正範, 日出間 純, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (58) 54-54 2012/09/04

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  22. 9-4 葉の老化時のRubisco分解におけるオートファジーの貢献度の評価(9.植物の多量栄養素,2012年度鳥取大会)

    小野 佑樹, 和田 慎也, 泉 正範, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (58) 54-54 2012/09/04

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  23. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd Edition) Peer-reviewed

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Weindl, G., Weis, S.N., Wen, L., Wen, X., Wen, Y., Westermann, B., Weyand, C.M., White, A.R., White, E., Whitton, J.L., Whitworth, A.J., Wiels, J., Wild, F., Wildenberg, M.E., Wileman, T., Wilkinson, D.S., Wilkinson, S., Willbold, D., Williams, C., Williams, K., Williamson, P.R., Winklhofer, K.F., Witkin, S.S., Wohlgemuth, S.E., Wollert, T., Wolvetang, E.J., Wong, E., Wong, G.W., Wong, R.W., Wong, V.K.W., Woodcock, E.A., Wright, K.L., Wu, C., Wu, D., Wu, G.S., Wu, J., Wu, J., Wu, M., Wu, M., Wu, S., d Wu, W.K.K., Wu, Y., Wu, Z., Xavier, C.P.R., Xavier, R.J., Xia, G.-X., Xia, T., Xia, W., Xia, Y., Xiao, H., Xiao, J., Xiao, S., Xiao, W., Xie, C.-M., Xie, Z., Xie, Z., Xilouri, M., Xiong, Y., Xu, C., Xu, C., Xu, F., Xu, H., Xu, H., Xu, J., Xu, J., Xu, J., Xu, L., Xu, X., Xu, Y., Xu, Y., Xu, Z.-X., Xu, Z., Xue, Y., Yamada, T., Yamamoto, A., Yamanaka, K., Yamashina, S., Yamashiro, S., Yan, B., Yan, B., Yan, X., Yan, Z., Yanagi, Y., Yang, D.-S., Yang, J.-M., Yang, L., Yang, M., Yang, P.-M., Yang, P., Yang, Q., Yang, W., Yang, W.Y., Yang, X., Yang, Y., Yang, Y., Yang, Z., Yang, Z., Yao, M.-C., Yao, P.J., Yao, X., Yao, Z., Yao, Z., Yasui, L.S., Ye, M., Yedvobnick, B., Yeganeh, B., Yeh, E.S., Yeyati, P.L., Yi, F., Yi, L., Yin, X.-M., Yip, C.K., Yoo, Y.-M., Yoo, Y.H., Yoon, S.-Y., Yoshida, K.-I., Yoshimori, T., Young, K.H., Yu, H., Yu, J.J., Yu, J.-T., Yu, J., d Yu, L. and Yu, W.H. and Yu, X.-F., Yu, Z., Yuan, J., Yuan, Z.-M., nd Yue, B.Y.J.T., Yue, J., Yue, Z., Zacks, D.N., Zacksenhaus, E., Zaffaroni, N., Zaglia, T., Zakeri, Z., Zecchini, V., Zeng, J., Zeng, M., Zeng, Q., Zervos, A.S., Zhang, D.D., Zhang, F., Zhang, G., Zhang, G.-C., Zhang, H., Zhang, H., Zhang, H., Zhang, J., Zhang, J., Zhang, J., Zhang, J.-P., Zhang, L., Zhang, L., Zhang, L., Zhang, M.-Y., Zhang, X., Zhang, X.D., Zhang, Y., Zhang, Y., Zhang, Y., Zhang, Y., Zhang, Y., Zhao, M., Zhao, W.-L., Zhao, X., Zhao, Y.G., Zhao, Y., Zhao, Y., Zhao, Y.-X., Zhao, Z., Zhao, Z.J., Zheng, D., Zheng, X.-L., Zheng, X., Zhivotovsky, B., Zhong, Q., Zhou, G.-Z., Zhou, G., Zhou, H., Zhou, S.-F., Zhou, X.-J., Zhu, H., Zhu, H., Zhu, W.-G., Zhu, W., nd Zhu, X.-F., Zhu, Y., Zhuang, S.-M., Zhuang, X., Ziparo, E., Zois, C.E., Zoladek, T., Zong, W.-X., Zorzano, A., Zughaier, S.M.

    Autophagy 12 (1) 1-222 2012/04

    DOI: 10.1080/15548627.2015.1100356  

    ISSN: 1554-8635 1554-8627

  24. RBCS1A and RBCS3B, two major members within the Arabidopsis RBCS multigene family, function to yield sufficient Rubisco content for leaf photosynthetic capacity

    Masanori Izumi, Honami Tsunoda, Yuji Suzuki, Amane Makino, Hiroyuki Ishida

    JOURNAL OF EXPERIMENTAL BOTANY 63 (5) 2159-2170 2012/03

    DOI: 10.1093/jxb/err434  

    ISSN: 0022-0957

  25. 9-27 シロイヌナズナにおけるRBCS1AおよびRBCS3Bの変異が葉のRubisco量に与える影響の解析(9.植物の多量栄養素)

    泉 正範, 角田 穂奈美, 鈴木 雄二, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (57) 66-66 2011/08/08

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  26. Cyclobutane pyrimidine dimer (CPD) photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA

    Masaaki Takahashi, Mika Teranishi, Hiroyuki Ishida, Junji Kawasaki, Atsuko Takeuchi, Tomoyuki Yamaya, Masao Watanabe, Amane Makino, Jun Hidema

    PLANT JOURNAL 66 (3) 433-442 2011/05

    DOI: 10.1111/j.1365-313X.2011.04500.x  

    ISSN: 0960-7412

  27. 9-21 シロイヌナズナ個別暗処理葉におけるオートファジー葉緑体分解機構(9.植物の多量栄養素,2010年度北海道大会)

    和田 慎也, 石田 宏幸, 吉本 光希, 大隅 良典, 牧野 周

    日本土壌肥料学会講演要旨集 (56) 60-60 2010/09/07

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  28. 9-17 シロイヌナズナにおけるRubisco-containing body(RCB)形成の栄養要因に対する応答の解析(9.植物の多量栄養素,2010年度北海道大会)

    泉 正範, 牧野 周, 石田 宏幸

    日本土壌肥料学会講演要旨集 (56) 59-59 2010/09/07

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  29. Rubisco turnover and photosynthesis in a leaf

    Ishida, H, Suzuki, Y, Makino, A

    Nitrogen Assimilation in Plants,Research Signpost 2010

  30. 9-21 シロイヌナズナ切離葉における栄養要因がRubisco-containing body(RCB)の形成に及ぼす影響の解析(9.植物の多量栄養素,2009年度京都大会)

    泉 正範, 石田 宏幸, 牧野 周

    日本土壌肥料学会講演要旨集 (55) 71-71 2009/09/15

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  31. Autophagy of chloroplast proteins Invited

    Ishida H, Wada S

    Photosynthesis research (Japanese) 53 89-94 2008/12

  32. 10-13 膜小胞Rubisco-containing body (RCB)を介した液胞におけるRubiscoの分解(10.植物の代謝,2008年度愛知大会)

    泉 正範, 石田 宏幸, 牧野 周

    日本土壌肥料学会講演要旨集 (54) 100-100 2008/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  33. 10-15 シロイヌナズナの個葉・個体暗処理による老化誘導と、オートファジーによる葉緑体分解(10.植物の代謝,2008年度愛知大会)

    和田 慎也, 石田 宏幸, 吉本 光希, 大隅 良典, 前 忠彦, 牧野 周

    日本土壌肥料学会講演要旨集 (54) 100-100 2008/09/09

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  34. Analysis of causal factors involved in Rubisco degradation by reactive oxygen species (ROS) in leaves under chilling-light stress

    Ryouhei Nakano, Hiroyuki Ishida, Amane Makino, Tadahiko Mae

    PLANT AND CELL PHYSIOLOGY 48 S96-S96 2007

    ISSN: 0032-0781

  35. Changes in the amount of Rubisco, leaf nitrogen and chlorophyll, and chloroplast number in the senescing leaf of wild-type Arabidopsis thaliana and autophagy defected mutant, Atatg4a4b-I.

    Shinya Wada, Hiroyuki Ishida, Kohki Yoshimoto, Yoshinori Ohsumi, Amane Makino, Tadahiko Mae

    PLANT AND CELL PHYSIOLOGY 48 S196-S196 2007

    ISSN: 0032-0781

  36. Involvement of ATG-dependent autophagic process in degradation of stromal proteins of chloroplasts

    Hiroyuki Ishida, Kohki Yoshimoto, Daniel Reisen, Amane Makino, Yoshinori Ohsumi, Maureen Hanson, Tadahiko Mae

    PLANT AND CELL PHYSIOLOGY 48 S196-S196 2007

    ISSN: 0032-0781

  37. 10-11 シロイヌナズナにおける Rubisco-containing body (RCB) の可視化とオートファジーとの関連性の解析(10. 植物の代謝, 2006年度秋田大会講演要旨)

    石田 宏幸, 吉本 光希, Reisen Daniel, 牧野 周, 大隈 良典, Hanson Maureen, 前 忠彦

    日本土壌肥料学会講演要旨集 (52) 87-87 2006/09/05

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  38. 10-10 Rubisco 大サブユニットを44kDに分解する葉緑体プロテアーゼの精製とその特性解析(10. 植物の代謝, 2006年度秋田大会講演要旨)

    和田 慎也, 石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 (52) 87-87 2006/09/05

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  39. 10-9 植物種間差から見た低温ストレス下における活性酸素による Rubisco 分解(10. 植物の代謝, 2006年度秋田大会講演要旨)

    中野 良平, 石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 (52) 86-86 2006/09/05

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  40. Purification and identification of a protease that site-specifically degrades Rubisco-LSU in chloroplasts

    S Wada, H Ishida, A Makino, T Mae

    PLANT AND CELL PHYSIOLOGY 46 S35-S35 2005

    ISSN: 0032-0781

  41. 細胞内でのRubisco分解の場について再考する Invited

    石田 宏幸

    日本光合成研究会会報 (41) 22-24 2004/11

  42. Fragmentation of the large subunit of Rubisco by reactive oxygen species occurs in cucumber leaf discs chilled in the light

    R Nakano, H Ishida, A Makino, T Mae

    PLANT AND CELL PHYSIOLOGY 45 S161-S161 2004

    ISSN: 0032-0781

  43. 10-1 活性酸素によるRubisco-LSUの断片化はin vivoでも起こる(10.植物の代謝)

    中野 良平, 石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 (49) 90-90 2003/08/20

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  44. 10-6 コムギ葉の老化過程におけるRubisco分解 : 免疫電顕とcDNAマイクロアレイによる解析(10.植物の代謝)

    石田 宏幸, 千葉 啓, 西澤 直子, 矢崎 潤史, 石川 雅弘, 藤井 文子, 真保 佳納子, 島谷 善平, 長田 夕子, 橋本 晶子, 太田 智弥, 佐藤 友紀, 宮本 智佳子, 本多 幸子, 小島 恵一, 佐々木 卓治, 岸本 直己, 菊池 尚志, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 (49) 91-91 2003/08

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  45. Exclusion of Rubisco from chloroplasts by the vesicle during natural senescence of wheat leaves

    A Chiba, H Ishida, NK Nishizawa, A Makino, T Mae

    PLANT AND CELL PHYSIOLOGY 44 S181-S181 2003

    ISSN: 0032-0781

  46. Fe2+-catalyzed site-specific cleavage of the large subunit of ribulose 1,5-bisphosphate carboxylase close to the active site

    S Luo, H Ishida, A Makino, T Mae

    JOURNAL OF BIOLOGICAL CHEMISTRY 277 (14) 12382-12387 2002/04

    DOI: 10.1074/jbc.M111072200  

    ISSN: 0021-9258

  47. 5 葉緑体破砕液における暗所下でのRubisco大サブユニットの断片化について(東北支部講演会)

    国分 紀元, 石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 (48) 205-205 2002/03/25

    Publisher: 一般社団法人日本土壌肥料学会

    ISSN: 0288-5840

  48. Rubisco degradation in leaves of wheat during dark-induced senescence

    H Ishida, T Enomoto, A Chiba, A Makino, T Mae

    PLANT AND CELL PHYSIOLOGY 43 S66-S66 2002

    ISSN: 0032-0781

  49. Two possible mechanisms for the Fe2+-mediated site-specific cleavage of the large subunit of ribulose-1,5-bisphosphate caxboxylase/oxygenase

    S Luo, H Ishida, A Makino, T Mae

    PLANT AND CELL PHYSIOLOGY 43 S66-S66 2002

    ISSN: 0032-0781

  50. Fragmentation of the large subunit of Rubisco into the 44-kDa fragment in the lysates of chloroplasts from the primary leaves of wheat

    N Kokubun, H Ishida, A Makino, T Mae

    PLANT AND CELL PHYSIOLOGY 43 S67-S67 2002

    ISSN: 0032-0781

  51. Light stress and Rubisco degradation

    石田 宏幸, 牧野 周, 前 忠彦

    kagaku to seibutsu 38 8-9 2000/01

    Publisher: gakkai syuppan center

  52. DEGRADATION OF GLUTAMINE SYNTHETASE MEDIATED BY REACTIVE OXYGEN SPECIES IN ILLUMINATED CHLOROPLASTS

    ISHIDA Hiroyuki, ANZAWA Daisuke, MAKINO Amane, MAE Tadahiko

    40 s120-s120 1999/03

    ISSN: 0032-0781

  53. Is Rubisco in excess for photosynthesis? Invited Peer-reviewed

    Ishida, H, Makino, A, Mae, T

    Chemistry and Biology 37 (2) 113-114 1999/02

    DOI: 10.1271/kagakutoseibutsu1962.37.113  

  54. Fragmentation of the large subunit of ribulose-1,5-bisphosphate carboxylase by reactive oxygen species occurs near Gly-329

    H Ishida, A Makino, T Mae

    JOURNAL OF BIOLOGICAL CHEMISTRY 274 (8) 5222-5226 1999/02

    DOI: 10.1074/jbc.274.8.5222  

    ISSN: 0021-9258

  55. 12 コムギleaf segmentにおける光と酸化的ストレス条件下でのRubisco分解産物の出現について(東北支部講演会)

    清水 佐知子, 石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 45 357-357 1999

    Publisher: 一般社団法人日本土壌肥料学会

    DOI: 10.20710/dohikouen.45.0_357_2  

    ISSN: 0288-5840

  56. 10-2 単離葉緑体におけるRubiscoの分解 : 活性酸素によるRubisco大サブユニットの部位特異的な断片化と高次構造との関係(10.植物の代謝)

    石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 45 117-117 1999

    Publisher: 一般社団法人日本土壌肥料学会

    DOI: 10.20710/dohikouen.45.0_117_2  

    ISSN: 0288-5840

  57. Rubisco: Photosynthesis and its protein turnover

    Mae, T, Makino, A, Ishida, H

    Applied Biological Science. 5 (2) 79-87 1999

    Publisher: 大阪府立大学生物資源開発センタ-

    ISSN: 1341-5573

  58. 10-1 単離葉緑体におけるRubiscoの分解 : 光ストレス下でのRubisco大サブユニットの活性酸素による断片化とその切断部位の同定(10.植物の代謝)

    石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 44 90-90 1998

    Publisher: 一般社団法人日本土壌肥料学会

    DOI: 10.20710/dohikouen.44.0_90_1  

    ISSN: 0288-5840

  59. The large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is fragmented into 37-kDa and 16-kDa polypeptides by active oxygen in the lysates of chloroplasts from primary leaves of wheat.

    H Ishida, Y Nishimori, M Sugisawa, A Makino, T Mae

    PLANT PHYSIOLOGY 114 (3) 1054-1054 1997/07

    ISSN: 0032-0889

  60. FRAGMENTATION OF THE LARGE SUBUNIT OF RUBISCO BY ACTIVE OXYGEN IN WHEAT CHLOROPLASTS UNDER ILLUMINATION

    ISHIDA Hiroyuki, SHIMIZU Sachiko, MAKINO Amane, MAE Tadahiko

    38 s75 1997/03

    ISSN: 0032-0781

  61. 8 葉緑体破砕液中におけるRubisco大サブユニットの活性酸素による断片化(東北支部講演会)

    石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 43 318-318 1997

    Publisher: 一般社団法人日本土壌肥料学会

    DOI: 10.20710/dohikouen.43.0_318_2  

    ISSN: 0288-5840

  62. 10-10 葉の老化に伴う窒素転流の分子的基盤 : 葉緑体に局在するpeptidaseの精製とその酵素的性質(10.植物の代謝)

    鈴木 渉, 石田 宏幸, 寺田 文子, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 43 104-104 1997

    Publisher: 一般社団法人日本土壌肥料学会

    DOI: 10.20710/dohikouen.43.0_104_2  

    ISSN: 0288-5840

  63. The Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase is Fragmented into 37-kDa and 16-kDa Polypeptides by Active Oxygen in the Lysates of Chloroplasts from Primary Leaves of Wheat

    Hiroyuki Ishida, Yoshito Nishimori, Miki Sugisawa, Amane Makino, Tadahiko Mae

    Plant and Cell Physiology 38 (4) 471-479 1997

    Publisher: Japanese Society of Plant Physiologists

    DOI: 10.1093/oxfordjournals.pcp.a029191  

    ISSN: 0032-0781

  64. IMMUNOCHEMICAL ANALYSIS OF MULTIPLE SUBUNIT POLYPEPTIDES OF GLUTAMINE-SYNTHETASE IN METHIONINE SULFOXIMINE-SENSITIVE AND TOLERANT TOBACCO CELL-CULTURES

    T YAMAYA, H ISHIDA, K KAMACHI, K OJIMA

    PLANT AND CELL PHYSIOLOGY 31 (3) 325-331 1990/04

    ISSN: 0032-0781

Show all ︎Show first 5

Presentations 51

  1. Autophagy and nutrient recycling in plants International-presentation

    Finnish-Japanese symposium 2016 2016/09/05

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    本人が発表

  2. Autophagy provides substrates to amino acid catabolic pathways as an adaptive response to sugar starvation in Arabidopsis International-presentation

    The 17th International Congress on Photosynthesis Research 2016/08/07

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    本人が発表

  3. Autophagy of chloroplasts as an adaptive response to sugar starvation in Arabidopsis International-presentation

    International Conference on Arabidopsis 2016/06/29

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    本人が発表

  4. Chloroplast autophagy as an adaptive response to sugar starvation in Arabidopsis International-presentation

    Gordon Research conference ‘Mitochondria and Chloroplasts' 2016/06/19

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    本人が発表

  5. 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に与える影響の解析

    横浜諒, 和田慎也, 菅野圭一, 小島創一, 山谷知行, 牧野周, 石田宏幸

    日本植物生理学会 2016/03/18

  6. シロイヌナズナの光障害条件におけるオートファジーによる障害葉緑体の除去

    泉正範, 石田宏幸, 中村咲耶, 日出間純

    日本植物生理学会 2016/03/18

  7. 光障害を受けた葉緑体を選択的に除去するクロロファジーの特性について

    中村咲耶, 泉正範, 石田宏幸, 日出間純

    日本植物生理学会 2016/03/18

  8. 4-1-10 シロイヌナズナの光障害時においてオートファジーは異常葉緑体の除去に関わる(4-1 植物の多量栄養素,2016年度佐賀大会)

    泉 正範, 中村 咲耶, 石田 宏幸, 日出間 純

    日本土壌肥料学会講演要旨集 2016

  9. 4-1-15 異なる光環境におけるイネの栄養成長とオートファジー(4-1 植物の多量栄養素,2016年度佐賀大会)

    和田 慎也, 山内 雄太, 石田 宏幸, 牧野 周

    日本土壌肥料学会講演要旨集 2016

  10. イネの発芽過程におけるオートファジーの役割

    和田慎也, 中村 萌, 石田宏幸, 牧野 周

    日本土壌肥料学会 2015/09/09

  11. シロイヌナズナのRCBおよび葉緑体オートファジーにおけるATI(ATG8-interacting proteins)の役割について

    石田宏幸, 西村 翼, 泉 正範, Gad Galili, 牧野 周

    日本土壌肥料学会 2015/09/09

  12. イネの栄養成長と老化葉の窒素リサイクルにおけるオートファジーの役割の解析

    和田慎也, 林田泰和, 泉 正範, 来須孝光, 花俣 繁, 朽津和幸, 牧野 周, 石田宏幸

    日本土壌肥料学会 2015/09/09

  13. シロイヌナズナにおけるオートファジーが亜鉛欠乏時に果たす役割

    江口雅丈, 吉本光希, 木村和彦, 泉 正範, 和田慎也, 牧野 周, 石田宏幸

    日本土壌肥料学会 2015/09/09

  14. Establishment of monitoring methods for autophagy in rice reveals autophagic recycling of chloroplasts and root plastids during energy limitation

    Masanori Izumi, Jun Hidema, Shinya Wada, Eri Kondo, Takamitsu Kurusu, Kazuyuki Kuchitsu, Amane Makino, Hiroyuki Ishida

    日本植物生理学会 2015/03/16

  15. シロイヌナズナの亜鉛欠乏条件下におけるオートファジーの役割

    江口雅丈, 木村和彦, 和田慎也, 泉正範, 牧野周, 石田宏幸

    日本植物生理学会 2015/03/16

  16. シロイヌナズナの暗処理による炭素欠乏条件下でオートファジーは分岐鎖アミノ酸を代替呼吸基質として供給する

    弘田隆晃, 泉正範, 牧野周, 石田宏幸

    日本植物生理学会 2015/03/16

  17. イネの栄養成長と老化葉の窒素リサイクルにおけるオートファジーの役割の解析

    和田慎也, 林田泰和, 泉正範, 来須孝光, 花俣繁, 朽津和幸, 牧野周, 石田宏幸

    日本植物生理学会 2015/03/16

  18. 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に及ぼす影響の解析

    横浜諒, 和田慎也, 牧野周, 石田宏幸

    日本植物生理学会 2015/03/16

  19. シロイヌナズナにおける糖欠乏下でのオートファジーの役割の解析

    弘田隆晃, 泉正範, 牧野周, 石田宏幸

    日本土壌肥料学会 2014/09/09

  20. シロイヌナズナにおける葉緑体オートファジーの機能解析

    泉正範, 石田宏幸, 牧野周, 日出間純

    日本土壌肥料学会 2014/09/09

  21. 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に及ぼす影響の解析

    和田慎也, 横浜諒, 石田宏幸, 牧野周

    日本土壌肥料学会 2014/09/09

  22. シロイヌナズナにおけるオートファジーの欠損が各栄養素の欠乏時の生存と成長に及ぼす影響の解析

    江口雅丈, 泉正範, 牧野周, 石田宏幸

    日本土壌肥料学会 2014/09/09

  23. Roles of autophagy in chloropalst recycling International-presentation

    Gordon Research conference ‘Mitochondria and Chloroplasts' 2014/07/06

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    本人が発表

  24. 植物の栄養リサイクルと葉緑体のオートファジー

    石田宏幸

    2014年度 日本農芸化学会 シンポジウム 2014/03/28

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    本人が発表

  25. オートファジーの欠損がイネの栄養成長と窒素転流へ与える影響の解析

    林田泰和, 和田慎也, 来須孝光, 朽津和幸, 牧野周, 石田宏幸

    第55回日本植物生理学会 2014/03/18

  26. Autophagy for Photodamaged Chloroplast in Arabidopsis

    Masanori Izumi, Hiroyuki Ishida, Amane Makino, Jun Hidema

    第55回日本植物生理学会 2014/03/18

  27. Roles of autophagy in Rubisco degradation during leaf senescence International-presentation

    6th European workshop on leaf senescence 2013/10/14

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    本人が発表

  28. Identification of a rice autophagy defected mutant, OsATG7, and phenotypes in the life cycle International-presentation

    Shinya Wada, Yasukazu Hatyashida, Takamitsu Kurusu, Kazuyuki Kuchitsu, Amane Makino, Hiroyuki Ishida

    6th European workshop on leaf senescence 2013/10/14

  29. Identification of a rice autophagy defected mutant, OsATG7, and phenotypes in the life cycle International-presentation

    Masanori Izumi, Takaaki Hirota, Jun Hidema, Amane Makino, Hiroyuki Ishida

    6th European workshop on leaf senescence 2013/10/14

  30. イネオートファジー欠損変異体Osatg7 の生理解析- ( その1) 栄養成長期における個体生育について-

    和田慎也, 林田泰和, 来須孝光, 朽津和幸, 牧野周, 石田宏幸

    第59回日本土壌肥料学会 2013/09/13

  31. イネオートファジー欠損変異体Osatg7 の生理解析-(その2)葉の老化過程における窒素転流への影響について-

    林田泰和, 和田慎也, 来須孝光, 朽津和幸, 牧野周, 石田宏幸

    第59回日本土壌肥料学会 2013/09/13

  32. イネにおけるRubisco-containing body/ オートファジー系の可視化

    泉正範, 日手間純, 近藤依里, 牧野周, 石田宏幸

    第59回日本土壌肥料学会 2013/09/13

  33. Autophagy contributes to Rubisco degradation during leaf senescence in Arabidopsis

    小野佑樹, 和田慎也, 泉正範, 牧野周, 石田宏幸

    第54回日本植物生理学会 2013/03/21

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    本人がポスター発表

  34. シロイヌナズナのエネルギー利用におけるオートファジーの寄与について

    泉正範, 発表者, 日出間純, 牧野周, 石田宏幸

    第54回日本植物生理学会 2013/03/21

  35. Autophagy of chloroplasts during leaf senescence International-presentation

    10th International Congress on Plant Molecular Biology 2012/10/21

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    本人が発表。 国際学会の国外招待

  36. Rubisco分解におけるオートファジーの貢献度の評価

    小野佑樹, 和田慎也, 泉正範, 牧野周, 石田宏幸

    第58回日本土壌肥料学会 2012/09/04

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    本人が口頭発表

  37. シロイヌナズナ葉の栄養素リサイクルにおいてRCB/オートファジー系が担う役割の解析

    泉正範, 発表者, 日出間純, 牧野周, 石田宏幸

    第58回日本土壌肥料学会 2012/09/04

  38. Contribution of autophagy to Rubisco degradation during leaf senescence International-presentation

    Gordon Research conference ‘Plant Senescence' 2012/07

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    本人が発表

  39. Ishida, H., Izumi, M., Yoshimoto, K., Hanson, M.R., Makino, A. International-presentation

    Gordon Research conference ‘Mitochondria and Chloroplasts' 2010/07

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    本人が発表者である

  40. Izumi, M., Makino, A., Ishida, H. International-presentation

    Gordon Research conference ‘Mitochondria and Chloroplasts' 2010/07

  41. Wada, S., Ishida, H., Yoshimoto, K., Ohsumi, Y., Makino, A. International-presentation

    Gordon Research conference ‘Mitochondria and Chloroplasts' 2010/07

  42. 泉 正範、石田 宏幸、牧野 周

    日本土壌肥料学会年会 2009/09

  43. Visualization of Rubisco-containing bodies derived from chloroplasts in living cells of Arabidopsis International-presentation

    Ishida, H, Yoshimoto, K, Reisen, D, Makino, A, Ohsumi, Y, Hanson, M.R, Mae, T

    14th International congress of photosynthesis 2008/07

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    本人による発表

  44. Possible involvement of ATG-dependent autophagic process in accumulation and degradation of Rubisco-containing body International-presentation

    Ishida H, Yoshimoto K, Reisen D, Makino A, Ohsumi Y, Hanson MR, Mae T

    The 53rd NIBB Conference 'Dynamic Organelles in Plants' 2006/06

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    本人による発表

  45. Ribulose-1,5-bisphosphate carboxylase/oxygenase is excluded from chloroplasts by specific bodies in senescing wheat and rice leaves International-presentation

    Ishida, H, Chiba, A, Hikichi, R, Nishizawa, N.K, Makino, A, Mae, T

    13th International congress of photosynthesis 2004/09

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    本人による発表

  46. Fragmentation of the large subunit of ribulose-1,5-bisphosphate carboxylase by reactive oxygen species occurs in vivo International-presentation

    Nakano R, Ishida, H, Makino, A, Mae, T

    13th International congress of photosynthesis 2004/09

  47. 12 コムギleaf segmentにおける光と酸化的ストレス条件下でのRubisco分解産物の出現について(東北支部講演会)

    清水 佐知子, 石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 1999

  48. 10-2 単離葉緑体におけるRubiscoの分解 : 活性酸素によるRubisco大サブユニットの部位特異的な断片化と高次構造との関係(10.植物の代謝)

    石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 1999

  49. 10-1 単離葉緑体におけるRubiscoの分解 : 光ストレス下でのRubisco大サブユニットの活性酸素による断片化とその切断部位の同定(10.植物の代謝)

    石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 1998

  50. 8 葉緑体破砕液中におけるRubisco大サブユニットの活性酸素による断片化(東北支部講演会)

    石田 宏幸, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 1997

  51. 10-10 葉の老化に伴う窒素転流の分子的基盤 : 葉緑体に局在するpeptidaseの精製とその酵素的性質(10.植物の代謝)

    鈴木 渉, 石田 宏幸, 寺田 文子, 牧野 周, 前 忠彦

    日本土壌肥料学会講演要旨集 1997

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Research Projects 24

  1. オルガネラ分解システムの理解に基づくイネ群落における葉間窒素分配の改善

    石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2024/04/01 - 2028/03/31

  2. ピースミールクロロファジーの多様性と選択性賦与のメカニズム

    石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東北大学

    2022/04/01 - 2024/03/31

  3. ピースミールオートファジーを介したフレキシブルな葉緑体機能衰退のメカニズム

    石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(B)

    Category: 基盤研究(B)

    Institution: 東北大学

    2019/04/01 - 2023/03/31

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    葉緑体には窒素栄養の多くが分配されタンパク質として機能している。Rubiscoなどの主要な葉緑体タンパク質は葉の老化時や栄養飢餓条件下では分解され、転流窒素源や糖の代替呼吸基質として個体の成長や生存に重要なリソースとなる。オートファジーは様々な発生過程や飢餓などのストレス条件下で働く真核生物に普遍の細胞内分解システムである。葉緑体は特異小胞RCBとしてピースミールオートファジーにより液胞に運ばれ分解されるが、その詳細な機構は不明である。本研究では、葉緑体のピースミールオートファジーの機構について明らかにすることを目的とし、今年度は以下の解析をすすめた。 (1)オートファジー隔離膜による葉緑体包膜の選択的な認識機構の解析 先行研究で同定したレセプター候補遺伝子3種の欠損変異体の単離と、それらの変異体とgfs9-5変異体との2重変異体の作出を進め、そのうち2種類の2重変異体を獲得した。残り1種の変異体については交配後のF2の種子集団を得た。 (2)植物組織からの高純度なRCBおよびオートファゴソームの単離方法の検討 植物組織からの高純度なオートファゴソームの単離方法の確立を目指し、gfs9-5変異体から調製した細胞破砕画分を、OptiPrep の不連続密度勾配による超遠心分離に供し、葉緑体(プラスチド)の一部を内包するRCBとサイトゾルを内包する一般オートファゴソームを密度の違いに基づきそれぞれ分取した。純度に影響を及ぼすパラメータである破砕緩衝液の組成、超遠心分離の条件、免疫磁気ビーズの種類や免疫反応/洗浄液の組成などについて最適化を行った。

  4. ピースミールクロロファジーの多様性と選択性賦与のメカニズム

    石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東北大学

    2020/04/01 - 2022/03/31

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    今年度は以下の項目を中心に解析を進めた。 1. RCB経路に選択性を賦与するメカニズムの解析:RCBに関わるレセプター候補の共免疫沈降解析による同定を行うため、gfs9-5にFLAG-AtATG8a(野生型)と、AIM binding siteに変異を持つ変異型を発現させる形質転換体を作出し、それらの中から組換えタンパク質の発現量が高い系統を複数選抜した。また共免疫沈降に用いる抗体ビーズについて検討し、シロイヌナズナのタンパク質粗抽出画分で高い特異性を発揮する抗体ビーズを選定した。 2. 葉緑体本体からのRCB切り離しのメカニズムの解析:RCB形成における葉緑体の増殖分裂に関わる既知因子の役割について、分裂装置の主要な構成因子のノックアウト変異がRCB経路に及ぼす影響について調べた。その結果、葉緑体分裂が特に異常となり葉緑体が巨大化するarc変異体に加えて、ftszやpdv変異体においてもRCB形成が確認された。またgfs9-5変異体に新たな変異原処理を行い、RCBを蓄積しないサプレッサー変異体のスクリーニングを進め、複数の変異体を獲得した。 3. ピースミールクロロファジー経路の多様性、特にRCBとATI-PS bodyの関係性の解析: ATI1/2がノックアウト/ダウンされたATI-KDでは、野生型と同レベルにPB/RCBの蓄積が見られた。CT-DsRedで可視化されるプラスチド/葉緑体由来のPB/RCBの中で、ATI1-GFPシグナルを持つ小胞(ATI1-PS body)の割合は20%程度であった。以上の結果から、ピールミールクロロファジーの多様性が改めて示唆された。

  5. Molecular basis of recycling and quality control of chloroplasts by selective autophagy

    Ishida Hiroyuki, IZUMI Masanori, WADA Shinya, EGUCHI Masatake, HIROTA Takaaki, YOKOHAMA Ryo, NISHIMURA Tsubasa, NAKAMURA Sakuya, ISHIDA Hiromi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2015/04/01 - 2019/03/31

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    We aimed to reveal molecular mechanisms of selective autophagy of chloroplasts and obtained following results. (1) We revealed roles of known ATG8-interacting proteins, NBR1, ATG11, and ATI1/2 on the RCB (Rubisco-containing body) pathway and chlorophagy (whole-chloroplast autophagy). (2) We isolated novel mutants having abnormality on the RCB pathway. (3) We searched novel autophagy receptors which may act for chloroplast by co-immunoprecipitation and LC-MS/MS analysis.

  6. Molecular basis of recycling and quality control of chloroplasts by selective autophagy(Fostering Joint International Research)

    Ishida Hiroyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    Institution: Tohoku University

    2016 - 2019

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    In this study, with the aim of elucidating the molecular mechanism involved in selective autophagy of chloroplasts, we conducted analysis in collaboration with Professor Klaas van Wijk of Cornell University in the United States and obtained the following results. (1) We identified the causal gene of a novel mutant that has abnormality in the RCB pathway isolated by forward genetics. (2) We fractionated vesicles, Rubisco-containing bodies (RCBs) involved in chloroplast autophagy, by density gradient ultracentrifugation. (3) We searched novel autophagy receptors which may act for chloroplast by co-immunoprecipitation and LC-MS/MS analysis.

  7. Does the plant have an ATG 5- or ATG 7- independent autophagy pathway?

    Ishida Hiroyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    The core ATG genes which are essential for autophagy in yeast is conserved in all eukaryotic organisms including plants. However, since Arabidopsis mutants which lack core ATG complete their life cycle, the existence of novel autophagy pathways which are different from the conventional-type autophagy. In this study, we examined the existence of ATG-independent novel autophagy pathway in Arabidopsis. We found that the number and size of chloroplasts per cell, which is known to be degraded by autophagy during leaf senescence, decreased in atg5 or atg7 mutants. We also tried to establish novel visualization methods for ATG-independent autophagy pathways in Arabidopsis.

  8. Roles of autophagy in the life cycle of rice

    Ishida Hiroyuki, MAKINO Amane

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2012/04/01 - 2016/03/31

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    We aimed to reveal roles of autophagy in the life cycle of rice and obtained following results. (1) We isolated autophagy-defective rice mutants. (2) We established monitoring methods for autophagy by generating transgenic rice expressing fluorescent protein markers. (3) We found that autophagy-defective rice mutants show delayed reproductive growth and heading and they show male sterile phenotype due to incomplete development of pollen and anther. (4) We found that autophagy-defective rice mutants show reduced biomass production and nitrogen use efficiency due to suppression of chloroplast protein degradation and nitrogen remobilisation.

  9. 植物の栄養飢餓とオートファジー

    石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東北大学

    2013/04/01 - 2015/03/31

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    独立栄養生物である植物にとって、獲得した貴重な無機栄養素やエネルギーの体内での効率利用・リサイクルは、過酷な環境下での生存・成長戦略の一つとして重要な意味を持つ。本研究では真核生物に普遍的に備わる細胞自己分解システム・オートファジーや、オートファジーによる葉緑体タンパク質の特異的な分解経路(RCB経路)が、植物の栄養飢餓応答に果たす役割について明らかにすることを目的とし、今年度は以下の項目を中心に解析を進めた。 (1)各種無機栄養素の欠乏耐性とオートファジー シロイヌナズナのオートファジー欠損変異体(Atatg5-1, Atatg10-1)では、無機栄養素の中でも特に亜鉛の欠乏条件下で下位葉の枯死や生存率の低下が顕著であった。野生体では亜鉛欠乏によりオートファジーの誘導が確認された。atg変異体では、野生体に対して培地からの亜鉛の吸収量や葉や根への分配比に差がないことから、オートファジーに依存した亜鉛の細胞内リサイクルが亜鉛欠乏耐性に重要な役割を果たしていることが示唆された。 (2)光合成制限下でのエネルギー供給系としてのオートファジーの役割 光合成制限下ではオートファジーが糖に代わる呼吸基質としてのアミノ酸供給に寄与していることが示唆されている。今年度はAtatg変異体、アミノ酸異化代謝系酵素の欠損変異体(ivdh-1, d2hgdh1-2, etfqo-1, gdh1-2 gdh2-1)、及びそれらの多重変異体の暗処理下での表現形や遊離アミノ酸プールの変動を解析することで、オートファジーとアミノ酸異化代謝系の関係性を示した。

  10. 植物の栄養飢餓とオートファジー

    石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 新学術領域研究(研究領域提案型)

    Category: 新学術領域研究(研究領域提案型)

    Institution: 東北大学

    2011/04/01 - 2013/03/31

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    本研究では真核生物に普遍的に備わる細胞自己分解システム「オートファジー」が、植物の栄養飢餓応答に果たす役割についてモデル植物であるシロイヌナズナを中心材料に分子レベルで明らかにすることを目的とした。本年度は特に以下の2項目について成果が得られた。 1)光合成制限環境下におけるエネルギー供給源としてのRCB/オートファジーの役割 葉や個体レベルでの栄養条件を様々な外的、内的条件で変化させ、それらが小胞RCB(Rubisco-containing body)の形成に及ぼす影響について調べた。その結果、RCBの形成は葉の炭水化物含量と特に密接に結びついていることが示された。そこで、炭素制限環境におけるRCB/オートファジーの役割について解析するため、オートファジーとスターチ代謝関連遺伝子の二重変異体の解析を進めた。スターチレス/オートファジー二重変異体では、短日条件において顕著な生育遅延が起き、生殖成長に至る前にほとんどの葉が枯死するというシビアな表現型が見られた。二重変異体では分岐鎖アミノ酸や芳香族アミノ酸含量が低下しており、これらアミノ酸の分解代謝に関わるIVDHやETF/ETFQO複合体のETFQOの遺伝子発現はスターチレス変異体や二重変異体で顕著に上昇していた。以上のことから、オートファジーが、炭素制限時にこれらアミノ酸分解代謝へのアミノ酸供給系として機能していることが示唆された。(Izumi et al., 2013, Plant Physiol.として発表 )。 2)葉の老化時のRubisco分解におけるRCB経路の貢献度の評価 RBCSと蛍光タンパク質(sGFP, mRFP)の融合タンパク質を発現する形質転換体を用いてRCB経路を定量評価する系を確立した。(Ono et al., 2013, Plant Cell Environ.として発表)。

  11. Mechanisms of chloroplast degradation by two-different autophagic pathways in senescent leaves

    ISHIDA Hiroyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2010 - 2011

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    I studied membrane dynamics and related genes among chloroplast autophagy that occurs in senescent leaves. Results suggested that (i)unlike RCBs, chloroplasts are transferred into the vacuolar lumen by invagination of tonoplasts ; (ii)in addition to ATG4, ATG2, 5, 7, and 10 are required for the chloroplast autophagy.

  12. An ingenious strategy for plant survival : Degradation of chloroplast proteins via autophagy and RCB vesicles

    ISHIDA Hiroyuki, YOSIMOTO Kohki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

    Institution: Tohoku University

    2008 - 2010

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    We studied mechanisms and physiological functions of a degradation of chloroplast proteins by autophagy as a strategy of plant survival. It was revealed that (i) tye production of RCBs which contain stromal proteins of chloroplasts is dependent on autophagy; (ii) the RCB production is closely related to leaf carbon status; (iii) autophagy negatively regulates leaf senescence by controlling salicylic acid signaling and the production of reactive oxygen species.

  13. Molecular genetic analysis of Rubisco degradation to maintain leafphotosynthesis

    ISHIDA Hiroyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2008 - 2009

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    I performed genetic analysis of the degradation mechanism of CO2-fixing enzyme, Rubisco, in senescing leaves, which is closely related to plant growth through photosynthesis and nutrient recycling. I isolated mutants which show abnormal stromule formation and mutants which delay the decrease in Rubisco content during leaf senescence. In addition, I revealed the mechanism of Rubisco degradation by autophagy.

  14. 栄養素のリサイクル源としての葉緑体タンパク質の分解機構の解析

    石田 宏幸, 牧野 周

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特定領域研究

    Category: 特定領域研究

    Institution: 東北大学

    2007 - 2008

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    本研究では栄養素のリサイクル機構を通して植物の環境応答性と深く関わる葉緑体タンパク質の分解機構について、主に葉緑体、液胞、および膜輸送系の動態の面から明らかにすることを目的とし、本年度は以下の2項目について実験を行った。 (1) RCB形成シグナルの解析 先に確立した葉緑体移行GFP発現シロイヌナズナと共焦点レーザー顕微鏡を用いたRCBの生葉における可視化法を駆使して、RCB経路の発現が、葉齢や生長段階(栄養生長と生殖生長)、窒素栄養や光条件によってどう調節されているのかについて解析した。切離葉においては、RCBは暗所や光合成が阻害されたりする状況下、すなわち炭水化物が枯渇する条件で多く蓄積した。 (2) 野生体およびatg変異体における葉緑体及び主要葉緑体タンパク質の消長の実態解析 atg変異体では野生体と比較して老化時のクロロフィルの減少が早く起こっていた。一方、Rubiscoについては老化初期に減少速度が遅延するものの、老化過程全体では野生体と同様の減少速度を示した。またatg変異体では葉緑体数は老化後期まで一定レベルに保たれていることがわかった。さらに老化が促進される条件である個葉暗処理下では、野生体ではRCBや葉緑体が液胞に輸送され、葉緑体数、サイズが減少するのに対し、atg変異体ではそのような移行は観察されず、処理期間を通して葉緑体数、サイズはほぼ一定に保持されていた。個葉暗処理下におけるRubiscoやクロロフィルの消長は自然老化過程とほぼ同様であった。

  15. 新規の葉緑体タンパク質分解系:RCB経路の分子機構

    石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 若手研究(B)

    Category: 若手研究(B)

    Institution: 東北大学

    2006 - 2007

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    本研究では、窒素転流機構と光合成機能維持の両面から植物の生長に深く関わる老化葉における葉緑体タンパク質の分解機構について分子レベルで明らかにすることを目的とし、本年度は以下の2項目について実験を行った。 (1)RCB経路に関わるプロテオーム解析 先に確立した葉緑体移行GFP発現シロイヌナズナと共焦点レーザー顕微鏡を用いたRCBの生葉における可視化法を用いて、RCBが蓄積した液胞を単離し、これとRCBが蓄積していない液胞を2次元電気泳動に供し、両者で差異の見られるポリペプチドのスポット群を特定することを試みた。まずRCBを含む液胞を単離する方法について検討した。RCBを形成させた葉からセルラーゼとペクチナーゼを用いた酵素法によりプロトプラストを単離した。その後、プロトプラストを破砕し液胞を遊離させた後、パーコールによる密度勾配超遠心法により液胞を単離した。単離した液胞にはRCBの存在が確認された。しかし、液胞画分には少量ながら葉緑体の混入が認められた。よってRCBのプロテオーム解析を行うためにはさらなる精製方法の確立が必要であることがわかった。 (2)ストロミュール形成変異体の選抜 葉緑体移行GFP発現シロイヌナズナの種子にEMS処理を行い、突然変異体のプールを作成した。この中からストロミュールの形成に異常の見られる変異体を共焦点レーザー顕微鏡により選抜する方法を確立した。17000個体のM2植物から、複数の変異個体を得た。さらに得られた変異個体について戻し交配、異なるエコタイプとの交配を進め、原因遺伝子同定のための材料を整備した。

  16. 老化に特異な新規構造体Rubisco-containing bodyの特性解析

    前 忠彦, 牧野 周, 石田 宏幸, 鈴木 雄二

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特定領域研究

    Category: 特定領域研究

    Institution: 東北大学

    2005 - 2006

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    葉緑体ストロマにターゲットされるGFPやDsRedを発現するシロイヌナズナ(CT-GFP,CT-DsRed)の緑葉を用いて、RCBの生細胞における可視化法について検討した。葉を個体から切離後、コンカナマイシンAあるいはE-64dを浸透させインキュベートすると、処理後10時間あたりからGFP蛍光をもつ小胞が液胞内に蓄積した。またCT-DsRedでも同様の結果が得られた。これらの小胞は直径が1μm前後でクロロフィルの自家蛍光を持たないなど、電顕におけるRCB像とよく似た特徴を有していた。抗GFP抗体及び抗RbcL (Rubisco-lATGe subunit)抗体を用いた二重免疫電顕による解析の結果、GFP小胞がRCBであることが確認された。このRCBの生細胞における可視化法を用いて、RCBの蓄積とオートファジー機構との関連性について調べた。その結果、オートファジーに必須の遺伝子ATGの欠損変異体(atg5-1)では、RCBの蓄積は全く見られないことがわかった。その代わりに、atg5-1では飢餓条件下でのインキュベート後、葉肉細胞において葉緑体から突出する管状の構造体、ストロミュールが出現していた。次にCT-DsRedとautophagosomeやautophagic bodyのマーカーであるGFP-ATG8aを共発現する形質転換体を用いて蛍光2重染色による解析を行った。阻害剤の存在下でインキュベートした葉では、CT-DsRedが葉緑体に加えて、液胞に存在するGFP-ATG8aで可視化された小胞、すなわちautophagic bodyにも局在することがわかった。またGFP-ATG8は稀に観察される短い球状のストロミュールにも局在していた。これらの結果から、RCBの蓄積はATGに依存したオートファジー機構により進行していることが示された。

  17. Molecular and physiological basis of Rubisco turnover and its relation to nitrogen nutrition

    MAE Tadahiko, MAKINO Amane, ISHIDA Hiroyuki, SUZUKI Yuji

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2003 - 2006

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    Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of Rubisco synthesized in rice leaves from emergence through senescence. The levels of rbcS and rbcL mRNAs, the amount of Rubisco synthesized, and the N influx greater for the 4 mM treatment than for the 1 mM treatment throughout the experiment. The mount of Rubisco synthesized was well correlated with the levels of both mRNAs during leaf expansion, but not after the completion of leaf expansion in both N treatments. The ratio of the amount of Rubisco synthesized to the levels of both mRNAs dramatically declined after full expansion. On the other hand, the amount of Rubisco synthesized was well correlated with the N influx in both N treatments. These results indicate that the N influx, namely, N availability, rather than the levels of rbcS and rbcL mRNAs, is more closely related to the amount of Rubisco synthesized in the leaf blade of rice throughout the life span of a leaf. Exclusion of Rubisco from chloroplasts by specific bodies in naturally senescing leaves of wheat Immunocytochemical electron-microscopic observation indicated that Rubisco and/or its degradation products are localized in small spherical bodies having a diameter of 0.4-1.2 μm in naturally senescing leaves of wheat. These Rubisco containing bodies (RCBS) were found in the cytoplasm and in the vacuoles,. RCBs contained another stromal protein, chloroplastic glutamine synthetase, but not thylaloid proteins. Ultrastructural analysis suggested that RCBs had double membranes, which seemed to be derived from the chloroplast envelop, and that RCBS were further surrounded by the other membrane structures in the cytoplasm. The appearance of RCBs was the most remarkable when the amount of Rubisco started to decrease at the early phase of leaf senescence. These results suggest that RCBS might be involved in the degradation process of Rubisco out side of chloroplasts during leaf senescence. In vivo frgmentation of the large subunit of Rubisco by reactive oxygen species in an intact leaf of Cucumber under chilling conditions. When lea discs of chilling-sensitive cucumber were illuminated at 4-℃, five major fragments of the LSU were observed. This fragmentation was completely inhibited by ROS scavengers and Tiron stimulated this fragmentation, whereas an iron-specific chelator, deforoxamine, suppressed it. Further, such fragments were identical to those generated from the purified Rubisco by an-OH-generating system in vitro on two-dimensional PAGE. These results indicate that the direct fragmentation of Rubisco by reactive oxygen species also occurs in an intact leaf.

  18. コムギ老化葉におけるRubiscoの膜小胞を介した葉緑体化への排出と分解

    石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 若手研究(B)

    Category: 若手研究(B)

    Institution: 東北大学

    2002 - 2004

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    RCBはRubiscoの分解が盛んな葉の老化初期に最も多く存在していることを先の研究において見出した。そしてRCBがリソソーム様のオルガネラに囲まれていること、およびRCBが液胞内に取り込まれていることを免疫電顕観察により見出した。この結果からRCBを介したRubiscoの葉緑体外への排出が葉の老化過程におけるRubisco分解において重要な役割を果たしていることが示唆されている。本年度は、コムギに加えて、モデル植物であるイネとアラビドプシスにおいてもRCBが見られるかどうか検討した。その結果、イネとアラビドプシスにおいてもRCBの存在が免疫電顕において確認された(Ishida et al.2005)。そこで葉緑体のストロマにターゲットされたGFPを発現するアラビドプシス形質転換体を用いて、RCBの生きた細胞内での可視化を試みた。その結果、液胞のATPase阻害剤であるコンカナマイシンAを加え、液胞内のpHを上昇させた葉の細胞において、RCBが液胞内に存在することを共焦点レーザー顕微鏡を用いて見出した。一方、コンカナマイシンA非存在下ではRCBは観察されなかった。共焦点レーザー顕微鏡において見出されたRCBは直径0.5-1.5マイクロメートルでありクロロフィル自家蛍光を有していなかった。この結果は電顕観察のものと一致した。以上の結果は、RCBが葉緑体外でのRubisco分解に関与していることを極めて強く示唆した(Ishida et al.投稿中)。

  19. Molecular basis for decline of leaf function during senescence: Analysis of major determinants causing decline of the amount of Rubisco

    MAE Tadahiko, ISHIDA Hiroyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2000 - 2001

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    1) Rubisco was actively synthesized during expansion, but the amount of Rubisco synthesized rapidly declined just before full expansion. It already declined to about one fifth of the maximal amount at the time of fall expansion, and further declined to about one-tenth during senescence. The changes in the levels of rbcL and rbcS mRNAs were almost coordinated with those changes in the amount of Rubisco synthesized. Thus, the amount of Rubisco synthesized was primarily determined by the levels of rbcL and rbcS mRNAs throughout the life span of the leaves. Degradation of Rubisco became far more active than synthesis of Rubisco during senescence. Since the synthesis of Rubisco during senescence scarcely contributed to its amount, the degradation of Rubisco is the major determinant for the amount of Rubisco in the senescent leaves. 2) The large subunit of Rubisco (LSU) was directly fragmented at Gly-329 into the 37-kDa and 16-kDa fragments by reactive oxygen species generated in the illuminated lysates of chloroplasts or intact chloroplasts. The LSU could also be cleaved into several other fragments by increasing the incubation time or the Fe^<2+> concentrations. All the cleavage sites were at or very close to the active site, just around the metal binding site within a radius of 12Å, indicating that proximity and favorable orientation are probably the most important parameter determining the cleavage sites. GS2 was also degraded by reactive oxygen species with the same manner as the LSU.

  20. コムギ葉における活性酸素を介した葉緑体タンパク質の分解とその分子機構

    石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 東北大学

    2000 - 2001

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    これまでの研究では、Rubisco大サブユニットが光照射下の単離葉緑体において活性酸素により直接断片化されることが明らかとなっていた。また同様の系においてRubiscoと同じストロマタンパク質であるGS2も活性酸素の1種であるヒドロキシルラジカルを介した反応により主に39,35,32,28kDaのフラグメントに断片化されることが見出された。両タンパク質とも精製標品をヒドロキシルラジカルの発生系にさらした際に、葉緑体と同様の分解を受けることが見出された。そこで本年度は、Rubisco及びGS2のヒドロキシルラジカルによる切断部位の同定を行った。Rubisco大サブユニットについては、6箇所の切断部位が同定され、それらはGly-404,Gly-380,Gly-329,Ala-296,Asp-203,Gly-122であった。これらの切断部位は活性部位に近接しており、金属結合部位から12Å以内の距離に位置していた(Luo et al. 2002)。一方、GS2については4つの分解フラグメントについてそれぞれN末端アミノ酸配列分析を行った。そのうちの35kDaおよび28kDaのフラグメントについてはN末端がブロックされており同定することはできなかった。残りの39kDaおよび32kDaフラグメントのN末端は、共に分解を受けていないGS2のN末端配列と一致した。これらの結果から、GS2の切断部位のうちの2つについてはN末端からそれぞれ39kDaおよび32kDa離れた位置に存在していることが明らかとなった(Ishida et al. 2002)

  21. Molecular Mechanisms of Degradation of chloroplast proteins during leaf senescence Competitive

    System: Basic Science Research Program

    1999/04 -

  22. 窒素転流と光合成機能に関わるRubisco分解の分子機構

    前 忠彦, 石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特定領域研究(A)

    Category: 特定領域研究(A)

    Institution: 東北大学

    1999 - 1999

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    1.コムギの場合に見出されたのと同じ分子量のRubisco大サブユニットの37kDaフラグメントが、ホウレンソウ、オオムギ、エンドウから単離した葉緑体を同様に処理した場合にも見出された。また、オオムギ及びイネから精製したRubiscoをヒドロキシルラジカル発生系(Fe^<2+>-H_2O_2-ascoribic acid)に曝した場合にも、37kDaフラグメントおよび16kDaフラグメントの出現が認められた。これらの結果は、ヒドロキシラジカルによるRubisco大サブユニットの部位特異的な切断化は,高等植物のRubiscoに共通に見られる現象であることを示唆していた。そこで既報のデーターに基づき切断部位近傍のアミノ酸配列について比較した。Gly-329はいずれの植物種においても保存されていたが、その両端のアミノ酸については、ホウレンソウとオオムギがコムギと同様にSとTで、エンドウはAとT、イネはAとAであった。現在、オオムギ、エンドウ、イネのそれぞれのフラグメントの末端アミノ酸についての同定を行っている。 2.葉緑体ストローマ酵素のGS2も光照射下の葉緑体およびその破砕液中で部位特異的に断片化されることが明白となった。すなわち、単離葉緑体及びその破砕液を光照射下におくと、GS2はRubisco同様、部位特異的に断片化された。そしてこの断片化は、、金属キレーターのEDTA,1,10-phenanthrolineにより完全に阻害され、セリンプロテアーゼ、アスパルティックプロテアーゼ、システインプロテアーゼに対する阻害剤によっては阻害されなかった。また活性酸素のスキャベンジャーでは、カタラーゼ、n-propyl gallateが顕著な阻害効果を示した。さらにこの断片化は、GSの阻害剤で酵素に強固に結合するmethionine sulfoximine(MSX)により完全に阻害された。これらの結果は、我々がRubiscoで見出した結果と全く同様であり、GS2が相同の機構により断片化されていることを強く示唆している。

  23. Physiological and biochemical studies on leaf development and senescence : Degradation of Rubisco mediated by reactive oxygen species

    MAE Tadahiko, ISHIDA Hiroyuki, MAKINO Amane

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: TOHOKU UNIVERSITY

    1997 - 1999

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    Rubilose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39, Rubiscso) is a bifunctional enzyme which catalyzes two competing reactions, photosynthetic COィイD22ィエD2 assimilation and photorespiratory carbon oxidation in the stroma of the chloroplasts, and is the most abundant protein in leaves. During leaf senescence or under environmental stress conditions, Rubisco is one of the early proteins that are broken down in leaves. However, only limited information is available on the triggering mechanisms that cause Rubisco degradation in plants. Here, we found that the large subunit of Rubisco is directly, fragmented into the 37-kDa and 16-kDa fragments by reactive oxygen species generated in the illuminated lysates of chloroplasts or intact chloroplasts. Analysis of the terminal amino acid residues of both fragments indicated that the fragmentation of the LSU occurs at Gly-329. Purified Rubisco, exposed to a hydroxyl radical generating system, was also cleaved at the same site. Reactive oxygen species generated at its catalytic site by a Fenton-type reaction may trigger the site specific degradation of the LSU in the light.

  24. コムギ葉緑体におけるRubisco分解の分子機構

    石田 宏幸

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 東北大学

    1998 - 1998

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  1. 日本学術振興会 特別研究員等審査会委員・専門委員

    2015/08/01 - 2017/07/31

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    日本学術振興会 特別研究員等の採用にかかる書面審査を行う

  2. 米国 科学研究費審査員

    2009/08 - 2010/10

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    研究者から応募された申請書のピアレビューを行う。

  3. 米国 科学研究費審査員

    2008/08 - 2008/10

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    研究者から応募された申請書のピアレビューを行う。

  4. オートファジーを可視化 イネ葉緑体の分解過程解明

    2015/04/24 -

  5. イネの葉緑体分解 追跡 東北大など 自食作用が関与

    2015/04/16 -

  6. 東北大など、イネもオートファジーで葉緑体を再利用、ライブセルイメージングで観察

    2015/04/14 -

  7. 東北大など 葉緑体の再利用過程を解明

    2015/04/10 -

  8. 稲の葉緑体再利用を可視化

    2015/04/10 -

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  1. 東北大学、学際科学国際高等研究センター「領域創成研究」

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    本研究では、コヒーレント光発生技術、分光技術、ラマン解析技術を利用することで、より簡便で正確なリン酸化・脱リン酸化タンパクの同定法、さらには非破壊法(細胞を壊さない)によるリン酸化タンパク質の動態検出方法を確立することを目指す。さらに、確立された技術を農学・生命科学の植物科学研究へと応用する。