Details of the Researcher

PHOTO

Sanggun Roh
Section
Graduate School of Agricultural Science
Job title
Professor
Degree

  • 博士（農学）（岩手大学）

  • 修士（農学）（ソウル大学）

e-Rad No.
90322130

Research History 11

  • 2021/04 - Present
    Tohoku University　Graduate School of Agricultural Science

  • 2009/04 - 2021/03
    Tohoku University　Graduate School of Agricultural Science　Associate Professor

  • 2020/04 - 2020/09
    京都大学大学院農学研究科　非常勤講師

  • 2017/11 - 2019/08
    Seoul National University　Adjunct Associate Professor

  • 2014/09 - 2014/09
    Tohoku University

  • 2003/12 - 2009/03
    Shinshu University　Faculty of Agriculture　Associate Professor

  • 2008/04 - 2009/02
    新潟大学大学院自然科学研究科　非常勤講師

  • 2004/06 - 2004/08
    Shinshu University

  • 1999/10 - 2003/11
    Shinshu University　Faculty of Agriculture　Assistant Professor

  • 2002/10 - 2002/12
    Shinshu University

  • 1997/05 - 1999/09
    Prince Henrys Institute of Medical Research　Post-doctor Research Fellow

Show all Show first 5

Education 3

  • Iwate University　The United Graduate School of Agricultural Sciences

    1994/04 - 1997/03

  • Seoul National University　College of Agricultural and Life Science,

    1991/03 - 1993/02

  • Seoul National University　Faculty of Agriculture

    1987/03 - 1991/02

Committee Memberships 7

  • 日本畜産学会　常務理事

    2023/04 - Present

  • Integrative Medicine Research　編集委員

    2017 - Present

  • 家畜栄養生理研究会　評議委員、編集委員

    2015/05 - Present

  • Journal of Animal Science and Technology　編集委員

    2014/01 - Present

  • Asian Australian Journal of Animal Science　編集委員

    2007/05 - Present

  • 家畜栄養生理研究会　庶務幹事

    2011/05 - 2013/04

  • 北信越畜産学会　会計監査

    2006/04 - 2007/03

Show all ︎Show first 5

Professional Memberships 10

  • 肉用牛研究会

    2015/03 - Present

  • 日本栄養食糧学会

    2014/03 - Present

  • アメリカ内分泌学学会

    2010/01 - Present

  • 日本産肉研究会会員

    2009/04 - Present

  • 東北畜産学会

    2009/04 - Present

  • 日本畜産学会

    2004/04 - Present

  • 日本分子生物学会

    2002/04 - Present

  • 日本比較内分泌学会

    2001/04 - Present

  • 家畜栄養生理研究会

    2001/04 - Present

  • American Society of Animal Science

    2000/01 - Present

︎Show all ︎Show first 5

Research Interests 9

  • Physiological parameter

  • Mammry Gland

  • Peripartum

  • Liver tissue

  • Growth Physiology

  • Japanese Black cattle

  • Weaning

  • Ruminant

  • Resilient Rumen

Research Areas 2

  • Life sciences / Veterinary medicine /

  • Life sciences / Animal production science /

Awards 7

  1. Animal Science Journal Excellent Paper Award

    2020/03

  2. Animal Science Journal Excellent Paper Award

    2017/03　日本畜産学会

  3. 伊藤記念財団賞

    2016/06/15　伊藤記念財団

  4. Animal Science Journal Reviewers Award

    2016/03/27　日本畜産学会

  5. AAAP-2nd Woogene B & G Award Protocol

    2012/11/27　Asian-Australian Society of Animal Production

  6. 日本農学進歩賞

    2007/11　財団法人農学会　家畜脂肪蓄積の分子機構の解明に関する研究

  7. 日本畜産学会奨励賞受賞

    2003/03　日本畜産学会

Show all ︎Show 5

Papers 148

  1. Methane emission prediction models for lactating cows based on feed intake, body weight, and milk yield and composition: Variable methane conversion factor-based approach

    Kohei Oikawa, Fuminori Terada, Mitsunori Kurihara, Tomoyuki Suzuki, Itoko Nonaka, Kenji Hosoda, Yuko Kamiya, Sanggun Roh, Satoshi Haga

    Journal of Dairy Science　108　(7)　7248-7261　2025/07

    Publisher: American Dairy Science Association

    DOI： 10.3168/jds.2025-26312 　

    ISSN： 0022-0302

  2. Regulation of IL-1β gene expression and its function of defense mechanism in rumen epithelial cells from pre- and postweaning calves

    Huseong Lee, Naoto Sugiyama, Koki Nishihara, Minji Kim, Satoshi Haga, Sanggun Roh

    Animal Bioscience　2025/05/19

    Publisher: Asian Australasian Association of Animal Production Societies

    DOI： 10.5713/ab.25.0042 　

    ISSN： 2765-0189

    eISSN： 2765-0235

  3. Dynamics of Blood Taurine Concentration and its Correlation with Nutritional and Physiological Status during the Fattening Period of Japanese Black Cattle

    Shuntaro Takai, Huseong Lee, Minji Kim, Shinichiro Torii, Nishihara Koki, Joonpyo Oh, Tatsunori Masaki, Kentaro Ikuta, Eiji Iwamoto, Kota Masuda, Yoshinobu Uemoto, Fuminori Terada, Satoshi Haga, Sanggun Roh

    Journal of Animal Science　2024/11/13

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/jas/skae347 　

    ISSN： 0021-8812

    eISSN： 1525-3163

    More details Close

    Abstract Taurine, biosynthesized from methionine or cysteine in the liver, plays a crucial regulatory role in bile acid conjugation, antioxidant effects, and glucose and cholesterol metabolism. This may influence the metabolic changes associated with fat accumulation in beef cattle. However, the physiological role of taurine in this species has not been fully elucidated. In this study, we explored the physiological role of taurine in Japanese Black steers (Bos taurus) in different phases during the fattening period. To examine the correlation among plasma taurine concentrations, various physiological parameters, and genes related to taurine synthesis in the liver, we used biopsied liver tissues, blood samples, and rumen fluids collected from 21 steers at three different stages, i.e., early (T1; 13 months of age), middle (T2; 20 months of age), and late (T3; 28 months of age) phases. Additionally, to investigate the regulatory mechanisms underlying the expression profile of taurine synthesis genes, primary bovine hepatocytes obtained from 4-week-old Holstein calves were treated with palmitate, oleate, acetate, propionate, or β-hydroxybutyrate (BHBA). Plasma taurine and cholesterol concentrations significantly (P < 0.001) increased in the T2 phase, which is potentially attributable to increased energy intake and assimilation induced by increased intake of concentrated feed. Cysteine sulfinic acid decarboxylase (CSAD) expression significantly increased (P < 0.01) in T2 than in other phases. The expression levels of cysteine dioxygenase type 1 (CDO1) and cholesterol 7 alpha-hydroxylase (CYP7A1) were significantly higher (P < 0.001) in T2 than in T3; moreover, the CDO1/glutamate-cysteine ligase catalytic subunit (GCLC) ratio was higher (P < 0.05) in T2 than in T1. Plasma taurine concentrations were positively correlated with plasma methionine (r = 0.51; P < 0.05) and total cholesterol (r = 0.56; P < 0.05) concentrations at T2. Relative CDO1 mRNA expression was upregulated in cultured bovine hepatocytes treated with oleate and propionate, whereas it was downregulated upon acetate treatment. These findings indicate that the increase in plasma taurine concentrations in the T2 phase is associated with changes in lipid and methionine metabolism in Japanese Black steers.

  4. Assessing the impact of three feeding stages on rumen bacterial community and physiological characteristics of Japanese Black cattle

    Huseong Lee, Minji Kim, Tatsunori Masaki, Kentaro Ikuta, Eiji Iwamoto, Koki Nishihara, Itoko Nonaka, Akane Ashihara, Youlchang Baek, Sungdae Lee, Yoshinobu Uemoto, Satoshi Haga, Fuminori Terada, Sanggun Roh

    Scientific Reports　14　(1)　2024/02/28

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-024-55539-y 　

    eISSN： 2045-2322

    More details Close

    Abstract In Japan, Japanese Black cattle, known for their exceptional meat quality owing to their abundant intramuscular fat, undergo a unique three-stage feeding system with varying concentrate ratios. There is limited research on physiological and rumen microbial changes in Japanese Black cattle during these stages. Therefore, this study aimed to examine Japanese Black steers in these three stages: early (T1, 12–14 months), middle (T2, 15–22 months), and late (T3, 23–30 months). The rumen bacteria of 21 cattle per phase was analyzed using 16S rRNA gene sequencing. Rumen bacterial diversity was significantly higher in T1, with a distinct distribution, than in T2 and T3. Specific phyla and genera were exclusive to each stage, reflecting the shifts in feed composition. Certain genera dominated each stage: T1 had Flexilinea, Streptococcus, Butyrivibrio, Selenomonas, and Kandleria; T2 had Bifidobacterium, Shuttleworthia, and Sharpea; and T3 had Acetitomaculum, Mycoplasma, Atopobium, and Howardella. Correlation analysis revealed significant associations between certain microbial populations and physiological parameters. These findings indicate that changes in energy content and feed composition are associated with physiological and ruminal alterations. This study may guide strategies to improve rumen health and productivity in Japanese Black cattle by modifying diets to specific fattening stages.

  5. Advances in Methane Emission Estimation in Livestock: A Review of Data Collection Methods, Model Development and the Role of AI Technologies

    Jalil Ghassemi Nejad, Mun-Su Ju, Jang-Hoon Jo, Kyung-Hwan Oh, Yoon-Seok Lee, Sung-Dae Lee, Eun-Joong Kim, Sanggun Roh, Hong-Gu Lee

    Animals　14　(3)　435-435　2024/01/29

    Publisher: MDPI AG

    DOI： 10.3390/ani14030435 　

    eISSN： 2076-2615

    More details Close

    This review examines the significant role of methane emissions in the livestock industry, with a focus on cattle and their substantial impact on climate change. It highlights the importance of accurate measurement and management techniques for methane, a potent greenhouse gas accounting for 14–16% of global emissions. The study evaluates both conventional and AI-driven methods for detecting methane emissions from livestock, particularly emphasizing cattle contributions, and the need for region-specific formulas. Sections cover livestock methane emissions, the potential of AI technology, data collection issues, methane’s significance in carbon credit schemes, and current research and innovation. The review emphasizes the critical role of accurate measurement and estimation methods for effective climate change mitigation and reducing methane emissions from livestock operations. Overall, it provides a comprehensive overview of methane emissions in the livestock industry by synthesizing existing research and literature, aiming to improve knowledge and methods for mitigating climate change. Livestock-generated methane, especially from cattle, is highlighted as a crucial factor in climate change, and the review underscores the importance of integrating precise measurement and estimation techniques for effective mitigation.

  6. TLR5 ligand induces the gene expression of antimicrobial peptides and CXCL8 through IL-1β gene expression in cultured rumen epithelial cells. International-journal

    Koki Nishihara, Yutaka Suzuki, Satoshi Haga, Sanggun Roh

    Animal science journal = Nihon chikusan Gakkaiho　95　(1)　e13972　2024

    DOI： 10.1111/asj.13972 　

    More details Close

    High grain feeding or weaning, which could compromise the rumen epithelium by increasing ruminal short-chain fatty acid (SCFA) concentrations with pH reduction, is associated with high levels of ruminal toll-like receptor 5 (TLR5). This study aimed to determine the role of TLR5 in the rumen epithelium. Immunohistochemistry revealed that TLR5 was localized in cells on the basal side (i.e., basal and spinous layers) rather than in the granular layer in the rumen epithelium, where tight junctions are most potent, in pre- and post-weaning calves (n = 9). Primary bovine rumen epithelial cells (BRECs) obtained from Holstein cows (n = 3) were cultured to investigate the factors that upregulate TLR5; however, SCFA, low pH (pH 5.6), BHBA, L-lactate, D-lactate, and LPS did not upregulate TLR5 gene expression in BREC. Primary BREC treated with flagellin (TLR5 ligand) had higher expression of interleukin-1β (IL-1β) (P < 0.05) than BREC treated with vehicle. In addition, BREC treated with IL-1β had higher expression of antimicrobial peptides and C-X-C motif chemokine ligand 8 than BREC treated with vehicle (P < 0.05). These results suggest that ruminal TLR5 may recognize epithelial disruption via flagellin and mediate the immune response via IL-1β during high-grain feeding or weaning.

  7. Effect of residual methane emission on physiological characteristics and carcass performance in Japanese Black cattle

    Minji Kim, Tatsunori Masaki, Kohei Oikawa, Akane Ashihara, Kentaro Ikuta, Eiji Iwamoto, Huseong Lee, Satoshi Haga, Yoshinobu Uemoto, Sanggun Roh, Fuminori Terada, Itoko Nonaka

    Animal Science Journal　95　(1)　2024/01

    Publisher: Wiley

    DOI： 10.1111/asj.13954 　

    ISSN： 1344-3941

    eISSN： 1740-0929

    More details Close

    Abstract This study investigated the physiological characteristics and carcass performance associated with residual methane emissions (RME), and the effects of bull differences on CH4‐related traits in Japanese Black cattle. Enteric methane (CH4) emissions from 156 Japanese Black cattle (111 heifers and 45 steers) were measured during early fattening using the sniffer method. Various physiological parameters were investigated to clarify the physiological traits between the high, middle, and low RME groups. CH4‐related traits were examined to determine whether bull differences affected progeny CH4 emissions. Ruminal butyrate and NH3 concentrations were significantly higher in the high‐RME group than in the low‐RME group, whereas the propionate content was significantly higher in the low‐RME group. Blood urea nitrogen, β‐hydroxybutyric acid, and insulin concentrations were significantly higher, and blood amino acids were lower in the high‐RME group than in the other groups. No significant differences were observed in the carcass traits and beef fat composition between RME groups. CH4‐related traits were significantly different among bull herds. Our results show that CH4‐related traits are heritable, wherein bull differences affect progeny CH4 production capability, and that the above‐mentioned rumen fermentations and blood metabolites could be used to evaluate enteric methanogenesis in Japanese Black cattle.

  8. Effects of Rumen Fermentation Characteristics on Stress-Related Hormones and Behavior in Sheep

    Qianrige, Sanggun Roh, Da-Hye Kim, Tetsuro Shishido, Shin-Ichiro Ogura

    Animals　13　(23)　3701-3701　2023/11/29

    Publisher: MDPI AG

    DOI： 10.3390/ani13233701 　

    eISSN： 2076-2615

    More details Close

    This study investigated the relationship between rumen fermentation, stress-related hormones, and behavior in sheep with the aim of providing insights for improving animal welfare and feed management practices. Eight lambs were assigned to either a high concentration or control group. Blood samples were collected for hormone analysis, and an open field test was conducted to observe behavioral stress responses. The results showed that diet composition may affect the behavior of ruminants in response to stressors and novel situations, as evidenced by the higher number of escape attempts in the high-concentration group. In addition, analyses of individual volatile fatty acids (VFAs) showed a significant positive correlation between the acetic acid/propionic acid ratio and sniffing behavior of the novel object (p &lt; 0.05, ρ = −0.414). These findings have important implications for animal welfare and feed management practices. Overall, this study provides insights into the potential impact of diet composition on the behavior of ruminants in response to stressors and novel situations, highlighting the importance of improving animal welfare through feed management practices. Further research is needed to fully elucidate the mechanisms underlying the complex relationship between rumen fermentation, stress-related hormones, and behavior in ruminant animals.

  9. Physiological roles and regulation of hepatic angiopoietin-like protein 3 in Japanese Black cattle (Bos taurus) during the fattening period. International-journal

    Rika Shikida, Minji Kim, Makoto Futohashi, Koki Nishihara, Huseong Lee, Yutaka Suzuki, Yeolchang Baek, Tatsunori Masaki, Kentaro Ikuta, Eiji Iwamoto, Yoshinobu Uemoto, Satoshi Haga, Fuminori Terada, Sanggun Roh

    Journal of animal science　2023/06/15

    DOI： 10.1093/jas/skad198 　

    More details Close

    Angiopoietin-like protein 3 (ANGPTL3) is expressed predominantly in the liver and plays a major role in regulating the circulating triglyceride and lipoprotein fraction concentrations by inhibiting lipoprotein lipase (LPL) activity. Given these physiological roles, ANGPTL3 may play an important role in metabolic changes related to fat accumulation during the fattening period in Japanese Black. This study aimed to reveal the physiological roles of hepatic ANGPTL3 in Japanese Black steers (Bos taurus) during the fattening period and investigate the regulatory effects of hepatic ANGPTL3. To investigate the gene expression and protein localization of ANGPTL3, 18 tissue samples were collected from tree male Holstein bull calves aged 7 weeks. Biopsied liver tissues and blood samples were collected from 21 Japanese Black steers during the early (T1; 13 months of age), middle (T2; 20 months), and late fattening phases (T3; 28 months). Relative mRNA expression, blood metabolite concentrations, hormone concentrations, growth, and carcass traits were analyzed. To identify the regulatory factors of hepatic ANGPTL3, primary bovine hepatocytes collected by 2 Holstein calves aged 7 weeks were incubated with insulin, palmitate, oleate, propionate, acetate, or beta-hydroxybutyric acid (BHBA). The ANGPTL3 gene was most highly expressed in the liver, with minor expression in the renal cortex, lungs, reticulum, and jejunum in Holstein bull calves. In Japanese Black steers, relative ANGPTL3 mRNA expressions were less as fattening progressed, and blood triglyceride, total cholesterol, and non-esterified fatty acid (NEFA) concentrations increased. Relative ANGPTL8 and Liver X receptor alpha (LXRα) mRNA expressions decreased in late and middle fattening phases, respectively. Furthermore, relative ANGTPL3 mRNA expression was positively correlated with ANGPTL8 (r = 0.650; P < 0.01) and ANGPTL4 (r = 0.540; P < 0.05) in T3 and T1, respectively, and LXRα showed no correlation with ANGPTL3. Relative ANGTPL3 mRNA expression was negatively correlated with total cholesterol (r = -0.434; P < 0.05) and triglyceride (r = -0.645; P < 0.01) concentrations in T3 and T1, respectively; There was no significant correlation between ANGTPL3 and carcass traits. Relative ANGTPL3 mRNA expression in cultured bovine hepatocytes was downregulated in oleate treatment. Together, these findings suggest that ANGPTL3 downregulation in late fattening phases is associated with the changes in lipid metabolism.

  10. Characteristics of Physiological Parameters of Japanese Black Calves Relate to Carcass Weight Peer-reviewed

    Shotaro Arakawa, Minji Kim, Tatsuya Aonuma, Michihiro Takagi, Satoshi Watanabe, Huseong Lee, Koki Nishihara, Satoshi Haga, Yoshinobu Uemoto, Sanggun Roh

    Animals　13　(3)　487-487　2023/01/31

    Publisher: MDPI AG

    DOI： 10.3390/ani13030487 　

    eISSN： 2076-2615

    More details Close

    This study aimed to identify the growth performance and blood factors associated with carcass weight in Japanese Black calves based on 675 performance tests and field carcass records. We measured the body weight, withers height, and chest girth at the start of fattening age (approximately 8–10 months) and analyzed eight blood factors, including vitamins and metabolites. Single- and two-trait animal models were used to estimate the heritability and genetic correlations. The heritability estimates for growth performance were moderate to high (ranging from 0.48 to 0.74), and those for blood metabolites were low to moderate (ranging from 0.19 to 0.51). Estimates for genetic correlations of carcass or body weight with body weight, withers height, and chest girth were high (ranging from 0.42 to 0.80). The body weight and withers height at 8 months of age are possibly closely related to the final carcass weight. The blood metabolites associated with body weight were vitamin E in steers (castrated males) and β-carotene in heifers. Our findings indicate that body measurements and blood metabolites measured during the growing period could be used to determine the nutritional and physiological status of cattle as well as predict carcass weight.

  11. Expression of chemerin in intestinal mucosa of calves with comparable expression level with other antimicrobial proteins Peer-reviewed

    Yutaka Suzuki, Kanako Kubota, Satoshi Haga, Hideaki Hayashi, Mutsumi Oishi, Hiroto Miura, Sanggun Roh, Satoshi Koike, Yasuo Kobayashi

    Animal Science Journal　93　(1)　e13750　2022/07

    Publisher: Wiley

    DOI： 10.1111/asj.13750 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  12. Physiological responses and adaptations to high methane production in Japanese Black cattle Peer-reviewed

    Minji Kim, Tatsunori Masaki, Kentaro Ikuta, Eiji Iwamoto, Koki Nishihara, Makoto Hirai, Yoshinobu Uemoto, Fuminori Terada, Sanggun Roh

    Scientific Reports　12　(1)　11154　2022/07

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41598-022-15146-1 　

    eISSN： 2045-2322

    More details Close

    Abstract In this study, using enteric methane emissions, we investigated the metabolic characteristics of Japanese Black cattle. Their methane emissions were measured at early (age 13 months), middle (20 months), and late fattening phases (28 months). Cattle with the highest and lowest methane emissions were selected based on the residual methane emission values, and their liver transcriptome, blood metabolites, hormones, and rumen fermentation characteristics were analyzed. Blood β-hydroxybutyric acid and insulin levels were high, whereas blood amino acid levels were low in cattle with high methane emissions. Further, propionate and butyrate levels differed depending on the enteric methane emissions. Hepatic genes, such as SERPINI2, SLC7A5, ATP6, and RRAD, which were related to amino acid transport and glucose metabolism, were upregulated or downregulated during the late fattening phase. The above mentioned metabolites and liver transcriptomes could be used to evaluate enteric methanogenesis in Japanese Black cattle.

  13. Changes in the liver transcriptome and physiological parameters of Japanese Black steers during the fattening period. International-journal Peer-reviewed

    Minji Kim, Tatsunori Masaki, Kentaro Ikuta, Eiji Iwamoto, Yoshinobu Uemoto, Fuminori Terada, Sanggun Roh

    Scientific reports　12　(1)　4029-4029　2022/03/07

    DOI： 10.1038/s41598-022-08057-8 　

    More details Close

    We investigated the physiological changes during the fattening period and production characteristics in Japanese Black steers bred and raised using the typical feeding system in Japan. Here, 21 Japanese Black steers aged 12 months were used, with experimental period divided into early (12-14 months of age), middle (15-22 months), and late fattening phases (23-30 months). The liver transcriptome, blood metabolites, hormones, and rumen fermentation characteristics were analyzed. Blood triglyceride and non-esterified fatty acid concentrations increased, whereas blood ketone levels decreased, with fattening phases. Blood insulin increased with fattening phases and was positively correlated with carcass weight and marbling in late fattening phases. Rumen fermentation characteristics showed high propionate levels and low butyrate levels in late fattening phases, likely due to increased energy intake. Genes related to glucose metabolism, such as SESN3, INSR, LEPR, and FOXO3, were down-regulated in late fattening phases. Genes related to lipid metabolism, such as FABP4, were up-regulated, whereas FADS1 and FADS2 were down-regulated. These findings suggest that the physiological changes resulted from changes in the energy content and composition of diets. Liver metabolism changed with changes in fat metabolism. Insulin was strongly associated with physiological changes and productivity in Japanese Black cattle.

  14. Maternal Nutrient Restriction Disrupts Gene Expression and Metabolites Associated with Urea Cycle, Steroid Synthesis, Glucose Homeostasis, and Glucuronidation in Fetal Calf Liver International-journal Peer-reviewed

    Susumu Muroya, Yi Zhang, Kounosuke Otomaru, Kazunaga Oshima, Ichiro Oshima, Mitsue Sano, Sanggun Roh, Koichi Ojima, Takafumi Gotoh

    Metabolites　12　(3)　203-203　2022/02/24

    Publisher: MDPI AG

    DOI： 10.3390/metabo12030203 　

    eISSN： 2218-1989

    More details Close

    This study aimed to understand the mechanisms underlying the effects of maternal undernutrition (MUN) on liver growth and metabolism in Japanese Black fetal calves (8.5 months in utero) using an approach that integrates metabolomics and transcriptomics. Dams were fed 60% (low-nutrition; LN) or 120% (high-nutrition; HN) of their overall nutritional requirements during gestation. We found that MUN markedly decreased the body and liver weights of the fetuses; metabolomic analysis revealed that aspartate, glycerol, alanine, gluconate 6-phosphate, and ophthalmate levels were decreased, whereas UDP-glucose, UDP-glucuronate, octanoate, and 2-hydroxybutyrate levels were decreased in the LN fetal liver (p ≤ 0.05). According to metabolite set enrichment analysis, the highly different metabolites were associated with metabolisms including the arginine and proline metabolism, nucleotide and sugar metabolism, propanoate metabolism, glutamate metabolism, porphyrin metabolism, and urea cycle. Transcriptomic and qPCR analyses revealed that MUN upregulated QRFPR and downregulated genes associated with the glucose homeostasis (G6PC, PCK1, DPP4), ketogenesis (HMGCS2), glucuronidation (UGT1A1, UGT1A6, UGT2A1), lipid metabolism (ANGPTL4, APOA5, FADS2), cholesterol and steroid homeostasis (FDPS, HSD11B1, HSD17B6), and urea cycle (CPS1, ASS1, ASL, ARG2). These metabolic pathways were extracted as relevant terms in subsequent gene ontology/pathway analyses. Collectively, these results indicate that the citrate cycle was maintained at the expense of activities of the energy metabolism, glucuronidation, steroid hormone homeostasis, and urea cycle in the liver of MUN fetuses.

  15. Maternal Nutrition During Gestation Alters Histochemical Properties, and mRNA and microRNA Expression in Adipose Tissue of Wagyu Fetuses Peer-reviewed

    Yi Zhang, Konosuke Otomaru, Kazunaga Oshima, Yuji Goto, Ichiro Oshima, Susumu Muroya, Mitsue Sano, Sanggun Roh, Takafumi Gotoh

    Frontiers in Endocrinology　12　2022/02/01

    Publisher: Frontiers Media SA

    DOI： 10.3389/fendo.2021.797680 　

    eISSN： 1664-2392

    More details Close

    We hypothesized that maternal low or high nutrition would give unique effects to morphological and molecular dynamics in adipose tissue of fetus of fatty breed Wagyu (Japanese Black) cattle which produce highly marbled beef. This study aimed to determine the effects of maternal energy intake in Wagyu cows, during gestation on fetal adipose tissue development, histochemical properties, and gene and microRNA (miRNA) expression. Cows were allocated to one of two nutritional energy groups: 120% (HIGH) or 60% nutritional requirements of (LOW). Fetuses (n = 6 per treatment) were removed from pregnant cows by cesarean section at fetal age 260 ± 8 days and euthanized. Subcutaneous adipose tissue (SAT), thoracic cavity visceral adipose tissue (TVAT), and perirenal adipose tissue (PAT) were collected for analysis. In histochemical analysis, in SAT and PAT, HIGH fetuses had greater diameter of adipocytes than LOW fetuses (P&amp;lt;0.05). Only in SAT, LOW fetuses had more Leptin (LEP) mRNA and tended to have more Peroxisome Proliferator-Activated Receptor gamma (PPARG) CCAAT-enhancer-binding proteins alpha (CEBPA) and Glucose transporter (GLUT) 4 mRNA(P&amp;lt;0.10). In all SAT, TVAT, and PAT, LOW fetuses had higher levels of the brown adipose tissue (BAT) biomarkers Uncoupling Protein (UCP) 1 and PPARG coactivator (PGC) 1α mRNA than HIGH fetuses (P&amp;lt;0.08). Meanwhile, in the other adipose tissue, LOW fetuses had lower PPARG, CEBPA, and Zinc Finger Protein (ZFP) 423 (in TVAT and PAT), FASN (in TVAT), LEP and GLUT4 mRNA (in PAT; P&amp;lt;0.10). In particular, in TVAT and PAT, LOW fetuses exhibited lower expression of WAT biomarkers (PPARG and ZFP423). Differential expression of various miRNAs related to adipogenesis between the LOW and HIGH fetuses was detected in an adipose tissue-specific manner (P&amp;lt;0.10). Based on adipose tissue-specific effects of maternal nutrition, these findings suggested that poor maternal nutrition in Wagyu cattle increased BAT development in SAT, TVAT and PAT, while elevated maternal nutrition stimulated fetal SAT development compared with that of TVAT and PAT.

  16. Genetic relationships of feed efficiency and growth traits with carcass traits in Japanese Shorthorn cattle International-journal Peer-reviewed

    Chiharu Shinoda, Jumpei Yasuda, Kousuke Yamagata, Keiichi Suzuki, Masahiro Satoh, Sanggun Roh, Yoshinobu Uemoto

    Animal Science Journal　93　(1)　e13691　2022/01

    Publisher: Wiley

    DOI： 10.1111/asj.13691 　

    ISSN： 1344-3941

    eISSN： 1740-0929

    More details Close

    In this study, we examined genetic parameters for feed efficiency, growth, and carcass traits in Japanese Shorthorn cattle, based on 714 performance tests and 15,790 field carcass records. Feed efficiency traits, including residual feed intake (RFI) and residual body weight gain (RG), were calculated. Single-trait and two-trait animal models were used to estimate heritability and genetic correlations. Heritability estimates for feed efficiency traits were found to be low to moderate (ranging from 0.03 to 0.36); notably, heritability was moderate for RG and low for RFI. Estimates for genetic correlations between feed efficiency traits and average daily gain (DG) were favorably moderate to high (absolute values of 0.43-0.85), and those with daily feed intake were low (absolute values of 0.00-0.32). We also estimated a high genetic correlation between RG and DG. The backfat thickness (BF) of bull calves showed favorable or no genetic correlation estimates with feed efficiency and growth traits, whereas RG and BF showed favorable or no genetic correlation estimates with carcass traits. Our findings indicate that genetic improvements in both feed utilization ability and carcass traits could be achieved by utilizing RG and BF in Japanese Shorthorn cattle.

  17. Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows Peer-reviewed

    Yutaka Suzuki, Sachi Chiba, Koki Nishihara, Keiichi Nakajima, Akihiko Hagino, Won-Seob Kim, Hong-Gu Lee, Tomonori Nochi, Toru Suzuki, Sang-Gun Roh

    Animals　11　(11)　3194-3194　2021/11/09

    Publisher: MDPI AG

    DOI： 10.3390/ani11113194 　

    eISSN： 2076-2615

    More details Close

    Epithelial barrier function in the mammary gland acts as a forefront of the defense mechanism against mastitis, which is widespread and a major disorder in dairy production. Chemerin is a chemoattractant protein with potent antimicrobial ability, but its role in the mammary gland remains unelucidated. The aim of this study was to determine the function of chemerin in mammary epithelial tissue of dairy cows in lactation or dry-off periods. Mammary epithelial cells produced chemerin protein, and secreted chemerin was detected in milk samples. Chemerin treatment promoted the proliferation of cultured bovine mammary epithelial cells and protected the integrity of the epithelial cell layer from hydrogen peroxide (H2O2)-induced damage. Meanwhile, chemerin levels were higher in mammary tissue with mastitis. Tumor necrosis factor alpha (TNF-α) strongly upregulated the expression of the chemerin-coding gene (RARRES2) in mammary epithelial cells. Therefore, chemerin was suggested to support mammary epithelial cell growth and epithelial barrier function and to be regulated by inflammatory stimuli. Our results may indicate chemerin as a novel therapeutic target for diseases in the bovine mammary gland.

  18. Maternal Undernutrition during Pregnancy Alters Amino Acid Metabolism and Gene Expression Associated with Energy Metabolism and Angiogenesis in Fetal Calf Muscle Peer-reviewed

    Susumu Muroya, Yi Zhang, Aoi Kinoshita, Kounosuke Otomaru, Kazunaga Oshima, Yuji Gotoh, Ichiro Oshima, Mitsue Sano, Sanggun Roh, Mika Oe, Koichi Ojima, Takafumi Gotoh

    Metabolites　11　(9)　582-582　2021/08/28

    Publisher: MDPI AG

    DOI： 10.3390/metabo11090582 　

    eISSN： 2218-1989

    More details Close

    To elucidate the mechanisms underlying maternal undernutrition (MUN)-induced fetal skeletal muscle growth impairment in cattle, the longissimus thoracis muscle of Japanese Black fetal calves at 8.5 months in utero was analyzed by an integrative approach with metabolomics and transcriptomics. The pregnant cows were fed on 60% (low-nutrition, LN) or 120% (high-nutrition, HN) of their overall nutritional requirement during gestation. MUN markedly decreased the bodyweight and muscle weight of the fetus. The levels of amino acids (AAs) and arginine-related metabolites including glutamine, GABA, and putrescine were higher in the LN group than those in the HN group. Metabolite set enrichment analysis revealed that the highly different metabolites were associated with the metabolic pathways of pyrimidine, glutathione, and AAs such as arginine and glutamate, suggesting that MUN resulted in AA accumulation rather than protein accumulation. The mRNA expression levels of energy metabolism-associated genes, such as PRKAA1, ANGPTL4, APLNR, CPT1B, NOS2, NOS3, UCP2, and glycolytic genes were lower in the LN group than in the HN group. The gene ontology/pathway analysis revealed that the downregulated genes in the LN group were associated with glucose metabolism, angiogenesis, HIF-1 signaling, PI3K-Akt signaling, pentose phosphate, and insulin signaling pathways. Thus, MUN altered the levels of AAs and expression of genes associated with energy expenditure, glucose homeostasis, and angiogenesis in the fetal muscle.

  19. Effects of low and high levels of maternal nutrition consumed for the entirety of gestation on the development of muscle, adipose tissue, bone, and the organs of Wagyu cattle fetuses Peer-reviewed

    Yi Zhang, Kounosuke Otomaru, Kazunaga Oshima, Yuji Goto, Ichiro Oshima, Susumu Muroya, Mitsue Sano, Rena Saneshima, Yukiko Nagao, Aoi Kinoshita, Yasuko Okamura, Sanggun Roh, Akira Ohtsuka, Takafumi Gotoh

    Animal Science Journal　92　(1)　2021/07

    Publisher: Wiley

    DOI： 10.1111/asj.13600 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  20. The Physiological Roles of Vitamin E and Hypovitaminosis E in the Transition Period of High-Yielding Dairy Cows Peer-reviewed

    Satoshi Haga, Hiroshi Ishizaki, Sanggun Roh

    Animals　11　(4)　1088-1088　2021/04/11

    Publisher: MDPI AG

    DOI： 10.3390/ani11041088 　

    eISSN： 2076-2615

    More details Close

    Levels of alpha-tocopherol (α-Toc) decline gradually in blood throughout prepartum, reaching lowest levels (hypovitaminosis E) around calving. Despite numerous reports about the disease risk in hypovitaminosis E and the effect of α-Toc supplementation on the health of transition dairy cows, its risk and supplemental effects are controversial. Here, we present some novel data about the disease risk of hypovitaminosis E and the effects of α-Toc supplementation in transition dairy cows. These data strongly demonstrate that hypovitaminosis E is a risk factor for the occurrence of peripartum disease. Furthermore, a study on the effectiveness of using serum vitamin levels as biomarkers to predict disease in dairy cows was reported, and a rapid field test for measuring vitamin levels was developed. By contrast, evidence for how hypovitaminosis E occurred during the transition period was scarce until the 2010s. Pioneering studies conducted with humans and rodents have identified and characterised some α-Toc-related proteins, molecular players involved in α-Toc regulation followed by a study in ruminants from the 2010s. Based on recent literature, the six physiological factors: (1) the decline in α-Toc intake from the close-up period; (2) changes in the digestive and absorptive functions of α-Toc; (3) the decline in plasma high-density lipoprotein as an α-Toc carrier; (4) increasing oxidative stress and consumption of α-Toc; (5) decreasing hepatic α-Toc transfer to circulation; and (6) increasing mammary α-Toc transfer from blood to colostrum, may be involved in α-Toc deficiency during the transition period. However, the mechanisms and pathways are poorly understood, and further studies are needed to understand the physiological role of α-Toc-related molecules in cattle. Understanding the molecular mechanisms underlying hypovitaminosis E will contribute to the prevention of peripartum disease and high performance in dairy cows.

  21. Evaluation of inbreeding and genetic diversity in Japanese Shorthorn cattle by pedigree analysis International-journal Peer-reviewed

    Yoshinobu Uemoto, Keiichi Suzuki, Jumpei Yasuda, Sanggun Roh, Masahiro Satoh

    Animal Science Journal　92　(1)　e13643　2021/01

    Publisher: Wiley

    DOI： 10.1111/asj.13643 　

    ISSN： 1344-3941

    eISSN： 1740-0929

    More details Close

    The Japanese Shorthorn is a Japanese Wagyu breed maintained at a small population size. We assessed the degree of inbreeding and genetic diversity among Japanese Shorthorn cattle using pedigree analysis. We analyzed the pedigree records of registered Japanese Shorthorn born between 1980 and 2018, after evaluating the pedigree completeness. The average of the actual inbreeding coefficients increased at the same rates annually from approximately 1.5% in 1980 to 4.2% in 2018 and was higher than the expected inbreeding coefficients over time. The effective population size based on the individual coancestry rate largely decreased from 127.8 in 1980 to 82.6 in 1999, and then remained almost constant at approximately 90. Three effective numbers of ancestors decreased over time until 1995, then remained almost constant. In particular, the effective number of founder genomes (Nge ) decreased from 43.8 in 1980 to 11.9 in 2018. The index of genetic diversity based on Nge decreased from 0.99 in 1980 to 0.96 in 2018 due to genetic drift in non-founder generations. Changes in inbreeding and genetic diversity parameters were similar between Japanese Shorthorn and other Japanese Wagyu breeds, but the magnitude of the changes was lower in the Japanese Shorthorn.

  22. Forestomach developmental failure in an 11-month-old Japanese Black steer with severely retarded growth and chronic ruminal tympany Peer-reviewed

    Tetsushi ONO, Keiichi HISAEDA, Yoichi INOUE, Yutaka YAMADA, Kenichi SHIBANO, Ikki MITSUI, Chizuka HENMI, Yumi UNE, Hideaki HAYASHI, Sanggun ROH, Masakatsu NOHARA, Eiji UCHIDA, Hajime NAGAHATA

    Journal of Veterinary Medical Science　83　(2)　220-225　2021

    Publisher: Japanese Society of Veterinary Science

    DOI： 10.1292/jvms.20-0448 　

    ISSN： 0916-7250

    eISSN： 1347-7439

    More details Close

    This study reports findings from the pathological examination of the forestomach of an 11-month-old Japanese Black steer with severely retarded growth (41% of expected weight) and chronic ruminal tympany. The ruminal papillae were weakly formed (0.3-0.5 cm long) and unevenly distributed. The cellulae and cristae reticuli were underdeveloped; the cristae were 0.4-0.7 cm in height and milky white. The keratinized layer in the stratified squamous epithelium was thickened. Ruminal pH was 5.25, and ruminal volatile fatty acid concentration was 11.7 mM. The steer's severely retarded growth was considered to be caused by malnutrition due to developmental and functional failure of the forestomach.

  23. Development of multipurpose recombinant reporter bovine leukemia virus International-journal Peer-reviewed

    Hironobu Murakami, Yusuke Yajima, Fumiaki Sato, Shinji Kamisuki, Satoshi Taharaguchi, Ken Onda, Sanggun Roh, Jumpei Uchiyama, Masahiro Sakaguchi, Kenji Tsukamoto

    Virology　548　226-235　2020/09

    Publisher: Elsevier BV

    DOI： 10.1016/j.virol.2020.07.011 　

    ISSN： 0042-6822

    More details Close

    Bovine leukemia virus (BLV) is a global problem that results in significant economic losses to the livestock industry. We developed three virus strains by inserting the HiBiT reporter tag from NanoLuc luciferase (NLuc) into limited sites within BLV molecular clones. Initial analysis for site selection of the tag insertion revealed a permissible site immediately downstream of the viral envelope gene. Therefore, NLuc activity could be used to measure virus copy numbers in the supernatant and the levels of cell infection. Productivity and growth kinetics of the reporter virus were similar to those of the wild-type strain; therefore, the reporter virus can be used to characterize the replication of chimeric viruses as well as responses to the antiviral drug, amprenavir. Collectively, our results suggest that the BLV reporter virus with a HiBiT tag insertion is a highly versatile system for various purposes such as evaluating virus replication and antiviral drugs.

  24. Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves Peer-reviewed

    Won-Seob Kim, Jalil Ghassemi Nejad, Sang-Gun Roh, Hong-Gu Lee

    Animals　10　(5)　895-895　2020/05/21

    Publisher: MDPI AG

    DOI： 10.3390/ani10050895 　

    eISSN： 2076-2615

    More details Close

    This study was conducted to investigate the effect of HS on HSPs gene expression in bovine PBMCs of beef calves in in vitro and in vivo models. In the in vitro experiment, blood samples were collected from the jugular vein of five beef calves (age: 174.2 ± 5.20 days, BW: 145.2 ± 5.21 kg). In the in vivo experiment, sixteen Korean native male beef calves (age: 169.6 ± 4.60 days, BW: 136.9 ± 6.23 kg) were exposed to ambient temperature for seven days (22 to 24 °C, relative humidity 60%; temperature–humidity index (THI) = 68 to 70) and subsequently to the temperature and humidity corresponding to the target THI level for 21 days (HS). For PBMC isolation, blood samples were collected every three days. In the in vitro model, the cell viability was significantly decreased in HS groups compared with the control group (p = 0.015). The expression of HSP70 (p = 0.022), HSP90 (p = 0.003) and HSPB1 (p = 0.026) genes was increased in the HS group in in vitro model. In the in vivo experiment, the HSP70 gene expression was increased after sudden exposure to HS conditions (severe THI levels; THI = 88 to 90), whereas HSP90 and HSPB1 showed no differences among the THI groups (p &gt; 0.05). However, in the severe THI group, the HSP70 gene expression returned to normal range after six days of continuous HS. In conclusion, the HSP70 gene plays a pivotal role in protecting cells from damage and is sensitive to HS in immune cells compared with other HSP genes in in vitro and in vivo models. In addition, the in vivo models suggest that calves exhibit active physiological mechanisms of adaptation to HS after six days of continuous exposure by regulating the HSP70 gene expression.

  25. Ruminal epithelial insulin‐like growth factor‐binding proteins 2, 3, and 6 are associated with epithelial cell proliferation Peer-reviewed

    Koki Nishihara, Yutaka Suzuki, Sanggun Roh

    Animal Science Journal　91　(1)　2020/01

    Publisher: Wiley

    DOI： 10.1111/asj.13422 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  26. Addition of Wakame seaweed (Undaria pinnatifida) stalk to animal feed enhances immune response and improves intestinal microflora in pigs Peer-reviewed

    Shimazu, T, L. Borjigin, K. Katoh, S. Roh, H. Kitazawa, K. Abe, Y. Suda, H. Saito, H. Kunii, K. Nihei, Y. Uemoto, H. Aso, K. Suzuki

    Animal Science Journal　90　(9)　1248-1260　2019/10

    DOI： 10.1111/asj.13274 　

    More details Close

    This study was conducted to evaluate the effects of supplementation of Wakame seaweed stalks on the immunity and intestinal microflora of pigs. Three separate experiments were performed: Relatively young (start at 20-30 kg; Experiments 1 and 2) and fattening period (70 kg; Experiment 3). All pigs (including the control group) were fed the same commercial feed, free from antibiotic additives, but in the feed for the treatment groups, 1% seaweed powder was added. There were no group differences observed in daily weight gain and feed intake in Experiments 1 and 2 between groups; however, daily weight gain was significantly higher in the treatment group compared to the control group in Experiment 3. The percentage of peripheral blood natural killer cells of the treatment group was significantly higher than that of the control group in all experiments. Although addition of seaweed changed the gene expression of cytokine and toll-like receptors of the small intestinal Peyer's patches slightly, seaweed seems to alter intestinal microflora preferentially, for instance, there was an increase in Lactobacillus and a decrease of Escherichia coli observed. These results suggest that Wakame seaweed can be used as supplement for pig feed to improve the gut health and immunity of pigs.

  27. ルーメン上皮の恒常性維持機構 Peer-reviewed

    西原 昂来, 盧 尚建

    栄養生理研究会報　63　(2)　27-34　2019/09

  28. Growth of rumen papillae in weaned calves is associated with lower expression of insulin-like growth factor-binding proteins 2, 3, and 6. International-journal Peer-reviewed

    Koki Nishihara, Yutaka Suzuki, Dahye Kim, Sanggun Roh

    Animal Science Journal　90　(9)　1287-1292　2019/09

    DOI： 10.1111/asj.13270 　

    More details Close

    This study aimed to characterize the relationship between the growth of rumen papillae in calves and the mRNA expression of insulin-like growth factor-binding proteins (IGFBPs) in the rumen papillae. The length of rumen papillae, the mRNA expression of IGFBPs in rumen papillae by quantitative real-time PCR, and the presence of insulin-like growth factors I and II (IGF-I and II) by immunohistochemistry (IHC) were analyzed in nine Holstein calves divided into three groups: suckling (2 weeks, n = 3), milk-continued (8 weeks, n = 3), and weaned (8 weeks, n = 3). The length of rumen papillae was greater (p < 0.01) in weaned calves than in suckling and milk-continued calves, whereas the expressions of IGFBP2, IGFBP3, and IGFBP6 genes were lower (p < 0.05) in the rumen papillae of weaned calves than in milk-continued calves. Thus, rumen papillae length and IGFBP2, 3, and 6 expressions were negatively correlated. The IHC analysis showed that IGF-I and IGF-II were present in the rumen epithelium of calves. These results suggested that the growth of rumen papillae after weaning is associated with the induction of IGFs by the low levels of IGFBP2, IGFBP3, and IGFBP6.

  29. Correlations between mitochondrial respiration activity and residual feed intake after divergent genetic selection for high and low oxygen consumption in mice International-journal Peer-reviewed

    Hongyu DARHAN, Atsushi ZODA, Motoi KIKUSATO, Masaaki TOYOMIZU, Kazuo KATOH, Sang-gun ROH, Shinichiro OGAWA, Yoshinobu UEMOTO, Masahiro SATOH, Keiichi SUZUKI

    Animal Science Journal　90　(7)　818-826　2019/04

    Publisher: Wiley

    DOI： 10.1111/asj.13210 　

    ISSN： 1344-3941

    eISSN： 1740-0929

    More details Close

    The aims of the present study were to identify the differences between two mouse lines (high (H)- and low (L)-oxygen consumption) in terms of mitochondrial respiratory activity when GMP (glutamate, malate, and pyruvate) and succinic acid are used as substrates and to examine the relationship between mitochondrial respiration activity and feed efficiency in both lines. The average daily feed intake, feed conversion ratio (FCR), and residual feed intake (RFI) were significantly higher in the H than the L line. The correlation between FCR and RFI was significant (r = 0.60, p < 0.05). RFI was effective as an indicator of feed efficiency. When succinic acid was used as a substrate, mitochondrial respiration states 2-4, ACR, and proton leak were significantly higher in the H than the L line. When GMP was used as a substrate, respiration states 3 and 4 in the H line were significantly higher than those in the L line, and there were significant positive correlations between FCR and RFI and mitochondrial respiration states 2-4. The results indicated that selection for high or low OC changed the basal metabolic rates estimated from liver mitochondrial respiration activity and feed efficiency.

  30. 和牛の胎仔発生に与える母体栄養の影響(Effects of maternal nutrition on fetal development in Wagyu cows)

    Yi Zhang, 實島 伶奈, 長尾 有希子, 木下 葵衣, 大島 一修, 後藤 裕司, 大島 一郎, 佐野 光枝, 室谷 進, 盧 尚建, 太箸 誠, 岡村 保子, 乙丸 孝之介, 後藤 貴文

    日本畜産学会大会講演要旨集　125回　169-169　2019/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  31. Effects of butyrate supplementation in antibiotic-free milk replacer and starter on growth performance in suckling calves. International-journal Peer-reviewed

    Sanggun Roh, Nobuhiro Kimura, Kazushi Sakamoto, Koki Nishihara, Keiichi Suzuki, Kazuo Katoh

    Animal Science Journal　89　(10)　1486-1491　2018/10

    DOI： 10.1111/asj.13096 　

    More details Close

    The aim of this study was to evaluate butyrate supplementation of antibiotic-free milk replacer and starter on growth performance in male Holstein calves. Twenty-nine calves were divided into two groups. Group C (n = 13) was fed antibiotic-free milk replacer without supplementation, and Group B (n = 16) was fed antibiotic-free milk replacer supplemented with butyrate (1.6 % DM of Gustor BP70® ). Starter in Group B contained 0.3 % DM of Gustor BP70® . The intake of milk replacer was lower in group B than in C (p = 0.07 for the treatment x week interaction). Body weight (BW) and heart girth (HG) in group B was higher than in C during the experimental period (p = 0.07 and 0.01 for the treatment × week interaction, respectively). The duration of the weaning period in group B was shorter than in group C (p = 0.02). β-hydroxybutyrate (BHBA) was higher in group B than in C (p = 0.04). Insulin like growth factor-1 (IGF-1) concentrations tended to be higher in group B than in C (p = 0.07 for treatment × week interaction). Our results show that butyrate supplementation in antibiotic-free milk replacer and starter exerted positive effects on growth performance in suckling calves.

  32. Downregulated angiopoietin-like protein 8 production at calving related to changes in lipid metabolism in dairy cows. International-journal Peer-reviewed

    Misato Nakano, Yutaka Suzuki, Satoshi Haga, Eri Yamauchi, Dahye Kim, Koki Nishihara, Keiichi Nakajima, Takafumi Gotoh, Seungju Park, Myunggi Baik, Kazuo Katoh, Sanggun Roh

    Journal of animal science　96　(7)　2646-2658　2018/06/29

    DOI： 10.1093/jas/sky162 　

    More details Close

    Acute physiological adaptation of lipid metabolism during the postpartum transition period of cows facilitates peripheral metabolic regulation. Hepatokines, which are hormones secreted from hepatocytes, are presumed to play a critical role in systemic metabolic regulation. Angiopoietin-like protein 8 (ANGPTL8) has been identified as a novel hepatokine associated with circulating triglyceride concentrations in mice and humans. However, regulation of ANGPTL8 and its physiological effects is still unknown in cattle. The present study aimed to reveal changes in ANGPTL8 expression and secretion during the periparturient period, and to investigate its regulatory effect on adipocytes and mammary epithelial cells. In the peripartum period, liver ANGPTL8 mRNA expression was lesser on the day of parturition and 1 wk postpartum than it was 1 wk before parturition (P < 0.05). Moreover, plasma ANGPTL8 concentrations decreased on the day of parturition as compared with that 1 wk before parturition (P < 0.05). In addition, ANGPTL8 expression in cultured bovine hepatocytes was downregulated after oleate and palmitate treatment but upregulated after insulin treatment (P < 0.05). ANGPTL8 decreased hormone-sensitive lipase (HSL) expression in differentiated adipocytes and cluster of differentiation 36 (CD36), fatty acid synthase (FAS), acetyl-coa carboxylase (ACC), and stearoyl-coa desaturase (SCD) in cultured bovine mammary epithelial cells (P < 0.05). These data suggest that hepatic ANGPTL8 production was downregulated postpartum when the cows experienced a negative energy balance. This downregulation was associated with increased concentrations of NEFA and decreased concentrations of insulin in lactating cows, and it facilitated lipid mobilization from adipose tissue to the mammary glands. We speculate that ANGPTL8 might have beneficial effects in reverting or improving the physiological adaptation and pathological processes of lipid metabolism during the peripartum period.

  33. Comparative transcriptome analysis of rumen papillae in suckling and weaned Japanese Black calves using RNA sequencing. International-journal Peer-reviewed

    Koki Nishihara, Daichi Kato, Yutaka Suzuki, Dahye Kim, Misato Nakano, Yu Yajima, Satoshi Haga, Miwa Nakano, Hiroshi Ishizaki, Ryouka Kawahara-Miki, Tomohiro Kono, Kazuo Katoh, Sang-Gun Roh

    Journal of animal science　96　(6)　2226-2237　2018/06/04

    DOI： 10.1093/jas/skx016 　

    ISSN： 0021-8812

    More details Close

    The length and density of rumen papillae starts to increase during weaning and growth of ruminants. This significant development increases the intraruminal surface area and the efficiency of VFA (acetate, propionate, butyrate, etc.) uptake. Thus, it is important to investigate the factors controlling the growth and development of rumen papillae during weaning. This study aimed to compare the transcriptomes of rumen papillae in suckling and weaned calves. Total RNA was extracted from the rumen papillae of 10 male Japanese Black calves (5 suckling calves, 5 wk old; 5 weaned calves, 15 wk old) and used in RNA-sequencing. Transcript abundance was estimated and differentially expressed genes were identified and these data were then used in Ingenuity Pathway Analysis (IPA) to predict the major canonical pathways and upstream regulators. Among the 871 differentially expressed genes screened by IPA, 466 genes were upregulated and 405 were downregulated in the weaned group. Canonical pathway analysis showed that "atherosclerosis" was the most significant pathway, and "tretinoin," a derivative of vitamin A, was predicted as the most active upstream regulator during weaning. Analyses also predicted IgG, lipopolysaccharides, and tumor-necrosis factor-α as regulators of the microbe-epithelium interaction that activates rumen-related immune responses. The functional category and the up-regulators found in this study provide a valuable resource for studying new candidate genes related to the proliferation and development of rumen papillae from suckling to weaning Japanese Black calves.

  34. Changes in the expression of α-tocopherol-related genes in liver and mammary gland biopsy specimens of peripartum dairy cows Peer-reviewed

    S. Haga, M. Miyaji, M. Nakano, H. Ishizaki, H. Matsuyama, K. Katoh, S. G. Roh

    Journal of Dairy Science　101　(6)　5277-5293　2018/06/01

    Publisher: Elsevier Inc.

    DOI： 10.3168/jds.2017-13630 　

    ISSN： 1525-3198 0022-0302

  35. Apelin administration to sheep promotes insulin secretion through increased AVP secretion. Peer-reviewed

    Katsuyoshi SATO

    Tohoku Journal of Animal Science and Technology　68　(1)　13-17　2018/06

  36. Sensory study of the effect of sweetener and seaweed addition on Japanese deer meat

    YAMANAKA Maho, HIRAYAMA Takuji, ROH Sang-Gun, HAYASHI Hideaki, KATOH Kazuo, SUZUKI Keiichi, OIKAWA Takuro

    Nihon Danchi Chikusan Gakkaihou　61　(2)　83-89　2018

    Publisher: Warm Regional Society of Animal Science, Japan

    DOI： 10.11461/jwaras.61.83 　

    ISSN： 2185-081X

    eISSN： 2185-1670

    More details Close

    Economic loss of agricultural and forestry resources has overwhelmingly increased in recent decades due to feeding damage of Japanese Deer （Cervus nippon centralis） in Japan. Utilization of a captured deer is becoming critical issue in relation to a management of the deer population. In this research, sensory research was carried out in order to investigate the consumer palatability for the deer meat processed by a sweetener （Trehalose and Sucrose） or seaweed （Wakame seaweed and Kombu）. The deer meat was investigated by panel test which consisted of 98 ordinary consumers. Sensory research was carried out using a questionnaire about taste, aroma, toughness, greasiness, juiciness and aggregate score of the meat, where each question was made of five choices. The deer meat processed by Wakame was evaluated significantly higher than the deer meat of no processing in all evaluation items. Interaction between the sex and the preference for deer meat was significant in taste score.

  37. Post-prandial decrease in plasma growth hormone levels is not related to the increase in plasma insulin levels in goats Peer-reviewed

    Koki Nishihara, Ryoko Kobayashi, Yutaka Suzuki, Katsuyoshi Sato, Kazuo Katoh, Sanggun Roh

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　30　(12)　1696-1701　2017/12

    DOI： 10.5713/ajas.16.0965 　

    ISSN： 1011-2367

    eISSN： 1976-5517

  38. Selection for high and low oxygen consumption-induced differences in maintenance energy requirements of mice Peer-reviewed

    Hongyu Darhan, Motoi Kikusato, Masaaki Toyomizu, Sang-gun Roh, Kazuo Katoh, Masahiro Sato, Keiichi Suzuki

    ANIMAL SCIENCE JOURNAL　88　(7)　959-965　2017/07

    DOI： 10.1111/asj.12740 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  39. Effects of mycoplasmal pneumonia of swine (MPS) lung lesion-selected Landrace pigs on MPS resistance and immune competence in three-way crossbred pigs Peer-reviewed

    Liushiqi Borjigin, Tomoyuki Shimazu, Yuki Katayama, Kouichi Watanabe, Haruki Kitazawa, Sang-gun Roh, Hisashi Aso, Kazuo Katoh, Masahiro Satoh, Yoshihito Suda, Akiko Sakuma, Mituru Nakajo, Keiichi Suzuki

    ANIMAL SCIENCE JOURNAL　88　(4)　575-585　2017/04

    DOI： 10.1111/asj.12698 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  40. Relationship of serum oxytocin concentration to positive social behaviors in cattle. Journal of Integrated Field Science Peer-reviewed

    Chen S, Ogura S, Roh S-G, Sato S

    Journal of Integrated Field Science　14　2017/03

    More details Close

    連絡著者

  41. Search for an index for the taste of Japanese Black cattle beef by panel testing and chemical composition analysis Peer-reviewed

    Keiichi Suzuki, Hiroyuki Shioura, Syoko Yokota, Kazuo Katoh, Sang-gun Roh, Fumiko Iida, Tomohiko Komatsu, Noriaki Syoji, Hironori Sakuma, Shinichi Yamada

    ANIMAL SCIENCE JOURNAL　88　(3)　421-432　2017/03

    DOI： 10.1111/asj.12663 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  42. ウシ組織におけるα－トコフェロール体内動態関連遺伝子の 発現特性に関する研究 Peer-reviewed

    芳賀 聡, 中野美和, 宮地 慎, 石崎 宏, 松山裕城, 鈴木 裕, 北山 峻, 小林洋介, 加藤和雄, 盧 尚建

    家畜栄養生理研究会報　61　(1)　9-19　2017/03/01

    Publisher:

    ISSN： 0286-4754

  43. Postweaning changes in the expression of chemerin and its receptors in calves are associated with the modification of glucose metabolism Peer-reviewed

    Y. Suzuki, S. Haga, M. Nakano, H. Ishizaki, M. Nakano, S. Song, K. Katoh, S. Roh

    JOURNAL OF ANIMAL SCIENCE　94　(11)　4600-4610　2016/11

    DOI： 10.2527/jas2016-0677 　

    ISSN： 0021-8812

    eISSN： 1525-3163

  44. 培養脂肪細胞におけるヨード高含有卵の卵黄の脂肪蓄積抑制の効果 Peer-reviewed

    横山 次郎, 蘇 敬夏, 鈴木 裕, 森岡 恵美, 鈴木 啓一, 加藤 和雄, 盧 尚建

    日本畜産学会報　87　(4)　345-350　2016/10/01

    Publisher: Japanese Society of Animal Science

    DOI： 10.2508/chikusan.87.345 　

    ISSN： 1346-907X

    eISSN： 1880-8255

  45. Iodine-enriched egg reduced total body fat and visceral fat among normal individuals: A placebo-controlled, randomized, double-blind study Peer-reviewed

    Yokoyama J, Morioka M, Inoue H, Yonei Y, Suzuki K, Katoh K, Roh SG

    Glycative Stress Research　3　(3)　172-176　2016/09/30

    Publisher: Society for Glycation Stress Research, Glycation Stress Research Center, Doshisha University

    DOI： 10.24659/gsr.3.3_172 　

    ISSN： 2188-3602

  46. Comparison of the gene expression of rumen epithelium in pre- and post-weaning young cattle Peer-reviewed

    Roh SG, Kato D, Suzuki Y, Haga S

    家畜栄養生理研究会報　60　(2)　79-84　2016/09/01

    Publisher:

    ISSN： 0286-4754

  47. Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach Peer-reviewed

    Soundharrajan Ilavenil, Da Hye Kim, Mayakrishnan Vijayakumar, Srisesharam Srigopalram, Sang Gun Roh, Mariadhas Valan Arasu, Jong Suk Lee, Ki Choon Choi

    BIOLOGICAL RESEARCH　49　(1)　38　2016/09

    DOI： 10.1186/s40659-016-0098-z 　

    ISSN： 0716-9760

    eISSN： 0717-6287

  48. Immunogenic properties and mycoplasmal pneumonia of swine (MPS) lung lesions in Large White pigs selected for higher peripheral blood immune capacity Peer-reviewed

    Liushiqi Borjigin, Tomoyuki Shimazu, Yuki Katayama, Meihua Li, Takumi Satoh, Kouichi Watanabe, Haruki Kitazawa, Sang-gun Roh, Hisashi Aso, Kazuo Katoh, Takafumi Uchida, Yoshihito Suda, Akiko Sakuma, Mituru Nakajo, Keiichi Suzuki

    ANIMAL SCIENCE JOURNAL　87　(5)　638-645　2016/05

    DOI： 10.1111/asj.12471 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  49. Utilization of digital differential display to identify differentially expressed genes related to rumen development Peer-reviewed

    Daichi Kato, Yutaka Suzuki, Satoshi Haga, KyoungHa So, Eri Yamauchi, Miwa Nakano, Hiroshi Ishizaki, Kichoon Choi, Kazuo Katoh, Sang-Gun Roh

    ANIMAL SCIENCE JOURNAL　87　(4)　584-590　2016/04

    DOI： 10.1111/asj.12448 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  50. 乳腺上皮細胞の酸化ストレス応答に及ぼすCCl4投与マウス血漿の影響

    河合真吾, 勝和華子, 今野芙実, 萩野顕彦, 盧尚建

    日本乳房炎研究会第２０回学術集会Proceedings　49-52　2016/03

  51. Immunogenic properties of Landrace pigs selected for resistance to mycoplasma pneumonia of swine Peer-reviewed

    Liushiqi Borjigin, Tomoyuki Shimazu, Yuki Katayama, Meihua Li, Takumi Satoh, Kouichi Watanabe, Haruki Kitazawa, Sang-gun Roh, Hisashi Aso, Kazuo Katoh, Takafumi Uchida, Yoshihito Suda, Akiko Sakuma, Mituru Nakajo, Keiichi Suzuki

    ANIMAL SCIENCE JOURNAL　87　(3)　321-329　2016/03

    DOI： 10.1111/asj.12440 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  52. Sensory research of soup of goat meat in Okinawa. Peer-reviewed

    Hirayama T, Hirakawa M, Oikawa T, Roh SG, Hayashi H

    J. Warm Regional Society of Anim. Sci.　59　(1)　17-21　2016/03/01

    Publisher: Warm Regional Society of Animal Science, Japan

    DOI： 10.11461/jwaras.59.17 　

    ISSN： 2185-081X

    More details Close

    Sensory research was carried out in order to investigate the consumer palatability ofgoat meat. One of traditional Okinawa dish, soup of goat meat was investigated by panel test. The sensoryresearch was conducted by 123 ordinary consumers (male: 83, female: 40). The survey research was carriedout based on a questionnaire about taste, aroma, toughness, and greasiness of the meat, where each questionwas made of five choices. Factors related to profile of the panelists were sex, age, place of origin and preferenceof the panelists were investigated. In toughness and greasiness, female panelists tended to evaluatethe meat more tough and greasy than male panelists (P < 0.05). In toughness, the panelists in age of 21-60class tended to evaluate the meat more tough than panelists of under 20 (P < 0.05). In aroma and aggregatescore, local panelists tended to evaluate the meat stronger smell than visitors (P < 0.05). This survey studyreveals preference of the panelists for goat soup is the most important factor to make favor/disfavor decision.This tendency was especially significant in evaluation of aroma, where more local panelists tend to favor thearoma of goat soup.

  53. Physiological Roles of Adipokines, Hepatokines, and Myokines in Ruminants Peer-reviewed

    Sang-Gun Roh, Yutaka Suzuki, Takafumi Gotoh, Ryuichi Tatsumi, Kazuo Katoh

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　29　(1)　1-15　2016/01

    DOI： 10.5713/ajas.16.0001R 　

    ISSN： 1011-2367

    eISSN： 1976-5517

  54. Mycoplasma pneumonia of swine (MPS) resistance and immune characteristics of pig lines generated by crossing an MPS pulmonary lesion selected Landrace line and a highly immune capacity selected Large White line. International-journal Peer-reviewed

    Borjigin L, Shimazu T, Katayama Y, Watanabe K, Kitazawa H, Roh S.G, Aso H, Katoh K, Uchida T, Suda Y, Sakuma A, Nakajo M, Suzuki K

    Animal Science Journal　87　(8)　972-981　2015/12/28

    DOI： 10.1111/asj.12529 　

    More details Close

    IF=1.045

  55. Effect of Suckling Systems on Serum Oxytocin and Cortisol Concentrations and Behavior to a Novel Object in Beef Calves Peer-reviewed

    Siyu Chen, Shigefumi Tanaka, Shin-ichiro Ogura, Sanggun Roh, Shusuke Sato

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　28　(11)　1662-1668　2015/11

    DOI： 10.5713/ajas.15.0330 　

    ISSN： 1011-2367

    eISSN： 1976-5517

  56. 反芻動物におけるヘパトカインとしてのChemerinとANGPTL8の発現調節 Peer-reviewed

    鈴木裕, 中野美智, 芳賀聡, 中島恵一, 加藤和雄, 盧尚建

    家畜栄養生理研究会　59　(2)　59-68　2015/11/01

  57. Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells Peer-reviewed

    Yutaka Suzuki, Satoshi Haga, Daiki Katoh, Kyoung-ha So, Ki-choon Choi, U-suk Jung, Hong-gu Lee, Kazuo Katoh, Sang-gun Roh

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　466　(3)　283-288　2015/10

    DOI： 10.1016/j.bbrc.2015.08.105 　

    ISSN： 0006-291X

    eISSN： 1090-2104

  58. Supplementation of long-chain fatty acids with lactating mammary glands and beef cow has potential benefits Peer-reviewed

    Riho Kurata, Xiaofeng Cui, Roh Sang-gun, Tomo Yonezawa

    2　(5)　00063　2015/08

  59. Single Nucleotide Polymorphism in the Coding Region of Bovine Chemerin Gene and Their Associations with Carcass Traits in Japanese Black Cattle Peer-reviewed

    Eri Yamauchi, Yutaka Suzuki, Kyoung-Ha So, Kei-ichi Suzuki, Kazuo Katoh, Sang-Gun Roh

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　28　(8)　1084-1089　2015/08

    DOI： 10.5713/ajas.14.0560 　

    ISSN： 1011-2367

    eISSN： 1976-5517

  60. Soluble extract of soybean fermented with Aspergillus oryzae GB 107 inhibits fat accumulation in cultured 3T3-L1 adipocytes Peer-reviewed

    Kyoung-Ha So, Yasuki Suzuki, Shinichi Yonekura, Yutaka Suzuki, Chan Ho Lee, Sung Woo Kim, Kazuo Katoh, Sang-Gun Roh

    NUTRITION RESEARCH AND PRACTICE　9　(4)　439-444　2015/08

    DOI： 10.4162/orp.2015.9.4.439 　

    ISSN： 1976-1457

    eISSN： 2005-6168

  61. Effects of dietary trans-9 octadecenoic acid, trans-11 vaccenic acid and cis-9, trans-11 conjugated linoleic acid in mice Peer-reviewed

    Tao Wang, Ji-Na Lim, Jae-Sung Lee, Sang-Bum Lee, Jin-Hee Hwang, U-Suk Jung, Min-Jeong Kim, Dae-Youn Hwang, Sang-Rak Lee, Sang-Gun Roh, Hong-Gu Lee

    MOLECULAR MEDICINE REPORTS　12　(2)　3200-3206　2015/08

    DOI： 10.3892/mmr.2015.3767 　

    ISSN： 1791-2997

    eISSN： 1791-3004

  62. Expression of alpha-tocopherol-associated genes and alpha-tocopherol accumulation in Japanese Black (Wagyu) calves with and without alpha-tocopherol supplementation Peer-reviewed

    S. Haga, M. Nakano, H. Ishizaki, S. G. Roh, K. Katoh

    JOURNAL OF ANIMAL SCIENCE　93　(8)　4048-4057　2015/08

    DOI： 10.2527/jas.2015-9106 　

    ISSN： 0021-8812

    eISSN： 1525-3163

  63. Proteomic Analysis Reveals PGAM1 Altering cis-9, trans-11 Conjugated Linoleic Acid Synthesis in Bovine Mammary Gland Peer-reviewed

    T. Wang, S. B. Lee, J. H. Hwang, J. N. Lim, U. S. Jung, M. J. Kim, H. S. Kang, S. H. Choi, J. S. Lee, S. G. Roh, H. G. Lee

    LIPIDS　50　(5)　469-481　2015/05

    DOI： 10.1007/s11745-015-4009-9 　

    ISSN： 0024-4201

    eISSN： 1558-9307

  64. Effects of fermented soybean meal on innate immunity-related gene expressions in nursery pigs acutely challenged with lipopolysaccharides Peer-reviewed

    Sang-Gun Roh, Jeffery A. Carroll, Sung Woo Kim

    ANIMAL SCIENCE JOURNAL　86　(5)　508-516　2015/05

    DOI： 10.1111/asj.12319 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  65. The Regulation of Oxytocin Receptor Gene Expression during Adipogenesis Peer-reviewed

    K. J. Yi, K. H. So, Y. Hata, Y. Suzuki, D. Kato, K. Watanabe, H. Aso, Y. Kasahara, K. Nishimori, C. Chen, K. Katoh, S. G. Roh

    JOURNAL OF NEUROENDOCRINOLOGY　27　(5)　335-342　2015/05

    DOI： 10.1111/jne.12268 　

    ISSN： 0953-8194

    eISSN： 1365-2826

  66. 乳腺組織の酸化ストレス応答に及ぼす抗酸化ビタミン類の影響

    勝 和華子, 河合真吾, 小﨑 幸, 萩野顕彦, 盧 尚建, 加藤和雄

    日本乳房炎研究会第19回学術集会Proceedings　45-48　2015/03

  67. Neurotensin Enhances Sperm Capacitation and Acrosome Reaction in Mice Peer-reviewed

    Yuuki Hiradate, Hiroki Inoue, Norio Kobayashi, Yoshiki Shirakata, Yutaka Suzuki, Aina Gotoh, Sang-gun Roh, Takafumi Uchida, Kazuo Katoh, Manabu Yoshida, Eimei Sato, Kentaro Tanemura

    BIOLOGY OF REPRODUCTION　91　(2)　1-9　2014/08

    DOI： 10.1095/biolreprod.113.112789 　

    ISSN： 0006-3363

    eISSN： 1529-7268

  68. Effect of single nucleotide polymorphisms in GH gene promoter region on carcass traits and intramuscular fatty acid compositions in Japanese Black cattle Peer-reviewed

    Haruna Sugita, Astrid Ardiyanti, Shoko Yokota, Shinichi Yonekura, Takuji Hirayama, Noriaki Shoji, Eri Yamauchi, Keiichi Suzuki, Kazuo Katoh, Sang-Gun Roh

    LIVESTOCK SCIENCE　165　15-21　2014/07

    DOI： 10.1016/j.livsci.2014.04.026 　

    ISSN： 1871-1413

    eISSN： 1878-0490

  69. Increased plasma ghrelin suppresses insulin release in wethers fed with a high-protein diet Peer-reviewed

    T. Takahashi, K. Sato, S. Kato, T. Yonezawa, Y. Kobayashi, Y. Ohtani, S. Ohwada, H. Aso, T. Yamaguchi, S. G. Roh, K. Katoh

    JOURNAL OF ENDOCRINOLOGY　221　(3)　371-380　2014/06

    DOI： 10.1530/JOE-13-0501 　

    ISSN： 0022-0795

    eISSN： 1479-6805

  70. Influences of protein ingestion on glucagon-like peptide (GLP)-1-immunoreactive endocrine cells in the chicken ileum Peer-reviewed

    Mohammad M. Monir, Kohzy Hiramatsu, Sachiko Matsumoto, Kei Nishimura, Chihiro Takemoto, Tomomi Shioji, Takafumi Watanabe, Kazumi Kita, Shinichi Yonekura, Sang Gun Roh

    ANIMAL SCIENCE JOURNAL　85　(5)　581-587　2014/05

    DOI： 10.1111/asj.12177 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  71. Immunological characteristics of peripheral blood leukocytes in swine selected for resistance to Mycoplasmal pneumonia Peer-reviewed

    Shimazu T, Borjigin L, Katayama Y, Li M, Satoh T, Watanabe K, Kitazawa H, Roh SG, Aso H, Kazuo K, Suda Y, Sakuma A, Nakajo M, Suzuki K

    Animal Science Journal　85　(4)　365373　2014/04

  72. Trigonelline attenuates the adipocyte differentiation and lipid accumulation in 3T3-L1 cells Peer-reviewed

    Soundharrajan Ilavenil, Mariadhas Valan Arasu, Jeong-Chae Lee, Da Hye Kim, Sang Gun Roh, Hyung Su Park, Gi Jun Choi, Vijayakumar Mayakrishnan, Ki Choon Choi

    PHYTOMEDICINE　21　(5)　758-765　2014/04

    DOI： 10.1016/j.phymed.2013.11.007 　

    ISSN： 0944-7113

    eISSN： 1618-095X

  73. ウシ乳腺上皮細胞の酸化ストレス応答に及ぼすNアセチルLシステインの影響

    萩野顕彦, 木下早織, 勝和華子, 盧 尚建, 加藤和雄

    日本乳房炎研究会第18回学術集会Proceedings　31-34　2014/03

  74. Actions of intravenous injections of AVP and oxytocin on plasma ACTH, GH, insulin and glucagon concentrations in goats Peer-reviewed

    Sang-Gun Roh, Kohta Koiwa, Katsuyoshi Sato, Yoshihisa Ohtani, Tatsuyuki Takahashi, Kazuo Katoh

    Animal Science Journal　85　(3)　286-292　2014/03

    DOI： 10.1111/asj.12142 　

    ISSN： 1344-3941 1740-0929

  75. Genetic selection for resistance to mycoplasmal pneumonia of swine (MPS) in the Landrace line influences the expression of soluble factors in blood after MPS vaccine sensitization Peer-reviewed

    Tomoyuki Shimazu, Liushiqi Borjigin, Yuki Katayama, Meihua Li, Takumi Satoh, Kouichi Watanabe, Haruki Kitazawa, Sang-gun Roh, Hisashi Aso, Katoh Kazuo, Yoshihito Suda, Akiko Sakuma, Mituru Nakajo, Keiichi Suzuki

    Animal Science Journal　85　(4)　365-373　2014

    Publisher: Blackwell Publishing

    DOI： 10.1111/asj.12158 　

    ISSN： 1740-0929 1344-3941

  76. In Vitro and In Vivo Enhancement of Adipogenesis by Italian Ryegrass (Lolium multiflorum) in 3T3-L1 Cells and Mice Peer-reviewed

    Mariadhas Valan Arasu, Soundharrajan Ilavenil, Da Hye Kim, Sang Gun Roh, Jeong-Chae Lee, Ki Choon Choi

    PLOS ONE　9　(1)　e85297　2014/01

    DOI： 10.1371/journal.pone.0085297 　

    ISSN： 1932-6203

  77. Individual difference in serum oxytocin concentrations of calves and the correlation with those in dams Peer-reviewed

    Siyu Chen, Shigefumi Tanaka, Chisako Oyakawa, Sanggun Roh, Shusuke Sato

    ANIMAL SCIENCE JOURNAL　85　(1)　53-57　2014/01

    DOI： 10.1111/asj.12082 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  78. Haplotype C of growth hormone (GH) gene in Japanese Black cattle: Structure of GH protein and a novel method for detection of the gene Peer-reviewed

    Abolfazl Bahrami, Astrid Ardiyanti, Kaoru Tonosaki, Keiichi Suzuki, Nanako Tanida, Takuji Hirayama, Sang-Gun Roh, Takeshi Nishio, Kazuo Katoh

    ANIMAL SCIENCE JOURNAL　84　(12)　802-804　2013/12

    DOI： 10.1111/asj.12132 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  79. Oxytocin Receptor in the Hypothalamus Is Sufficient to Rescue Normal Thermoregulatory Function in Male Oxytocin Receptor Knockout Mice Peer-reviewed

    Yoshiyuki Kasahara, Keisuke Sato, Yuki Takayanagi, Hiroaki Mizukami, Keiya Ozawa, Shizu Hidema, Kyoung-Ha So, Teruo Kawada, Nao Inoue, Ikuo Ikeda, Sang-Gun Roh, Keiichi Itoi, Katsuhiko Nishimori

    ENDOCRINOLOGY　154　(11)　4305-4315　2013/11

    DOI： 10.1210/en.2012-2206 　

    ISSN： 0013-7227

    eISSN： 1945-7170

  80. Immunological characterization of peripheral blood leukocytes using vaccine for mycoplasmal pneumonia of swine (MPS) in swine line selected for resistance to MPS Peer-reviewed

    Tomoyuki Shimazu, Liushiqi Borjigin, Yuki Katayama, Meihua Li, Takumi Satoh, Kouichi Watanabe, Haruki Kitazawa, Sang-gun Roh, Hisashi Aso, Kazuo Katoh, Yoshihito Suda, Akiko Sakuma, Mituru Nakajo, Keiichi Suzuki

    ANIMAL SCIENCE JOURNAL　84　(10)　683-692　2013/10

    DOI： 10.1111/asj.12058 　

    ISSN： 1344-3941

  81. Genetic polymorphisms and protein structures in growth hormone, growth hormone receptor, ghrelin, insulin-like growth factor 1 and leptin in Mehraban sheep Peer-reviewed

    A. Bahrami, Sh. Behzadi, S. R. Miraei-Ashtiani, S. -G. Roh, K. Katoh

    GENE　527　(1)　397-404　2013/09

    DOI： 10.1016/j.gene.2013.05.066 　

    ISSN： 0378-1119

    eISSN： 1879-0038

  82. Effect of nutrient intake on intramuscular glucose metabolism during the early growth stage in cross-bred steers (Japanese Black malexHolstein female) Peer-reviewed

    F. Ebara, S. Inada, M. Morikawa, S. -H. Asaoka, Y. Isozaki, A. Saito, T. Etoh, Y. Shiotsuka, S. G. Roh, J. Wegner, T. Gotoh

    JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION　97　(4)　684-693　2013/08

    DOI： 10.1111/j.1439-0396.2012.01310.x 　

    ISSN： 0931-2439

  83. 反芻動物の糖脂質代謝系におけるChemerinの生理的作用機構と生産性との関連 Peer-reviewed

    鈴木 裕, 北山 峻, 山内 恵利, 宋 相憲, 加藤 和雄, 盧 尚建

    家畜栄養生理研究会　57　(1)　45-54　2013/05

    Publisher:

    ISSN： 0286-4754

  84. ウシ培養肝細胞におけるChemerinの発現と調節因子 Peer-reviewed

    北山 峻, Ardiyanti Astrid, 山内 恵利, 後藤 貴文, 萩野 顕彦, 加藤 和雄, 盧 尚建

    日本畜産学会大会講演要旨集　116回　186-186　2013/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  85. A high-protein diet induces dissociation between plasma concentrations of growth hormone and ghrelin in wethers Peer-reviewed

    T. Takahashi, Y. Kobayashi, S. Haga, Y. Ohtani, K. Sato, Y. Obara, A. Hagino, S. G. Roh, K. Katoh

    JOURNAL OF ANIMAL SCIENCE　90　(13)　4807-4813　2012/12

    DOI： 10.2527/jas.2011-4596 　

    ISSN： 0021-8812

    eISSN： 1525-3163

  86. The Regulation of Chemerin and CMKLR1 Genes Expression by TNF-alpha, Adiponectin, and Chemerin Analog in Bovine Differentiated Adipocytes Peer-reviewed

    Y. Suzuki, Y. H. Hong, S. H. Song, A. Ardiyanti, D. Kato, K. H. So, K. Katoh, S. G. Roh

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　25　(9)　1316-1321　2012/09

    DOI： 10.5713/ajas.2012.12083 　

    ISSN： 1011-2367

  87. 反芻動物のストレス関連ホルモン分泌における下垂体前葉-後葉関連

    佐藤勝祥, 増子孝則, 折橋穀典, 萩野顕彦, 小原嘉昭, 盧 尚建, 加藤和雄

    畜産の研究　66　(9)　889-895　2012/09

  88. 反芻動物の培養肝細胞におけるchemerinの発現調節機構 Peer-reviewed

    北山 峻, 菊池 佑弥, Ardiyanti Astrid, 山内 恵利, 後藤 貴文, 萩野 顕彦, 加藤 和雄, 盧 尚建

    東北畜産学会報　62　(2)　25-25　2012/08

    Publisher: 東北畜産学会

    ISSN： 1341-626X

  89. Effects of Growth Hormone Gene Polymorphism on Lipogenic Gene Expression Levels in Diaphragm Tissues of Japanese Black Heifers Peer-reviewed

    Astrid Ardiyanti, Tsuyoshi Abe, Nanae Tameoka, Eiji Kobayashi, Noriaki Shoji, Yoshihisa Ohtani, Keiichi Suzuki, Sang-gun Roh, Kazuo Katoh

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　25　(8)　1055-1062　2012/08

    DOI： 10.5713/ajas.2012.12029 　

    ISSN： 1011-2367

  90. Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment Peer-reviewed

    Tomokazu Fukuda, Masafumi Katayama, Takayuki Yoshizawa, Takahiro Eitsuka, Hiroaki Mizukami, Kiyotaka Nakagawa, Hisao Ito, Homika Komagata, Sanghoun Song, Sanggun Roh, Yumi Hoshino, Eimei Sato, Hirofumi Hanada, Katsuhiko Nishimori, Teruo Miyazawa, Takafumi Uchida

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　76　(7)　1372-1377　2012/07

    DOI： 10.1271/bbb.120155 　

    ISSN： 0916-8451

    eISSN： 1347-6947

  91. Apelin is involved in postprandial responses and stimulates secretion of arginine-vasopressin, adrenocorticotropic hormone, and growth hormone in the ruminant Peer-reviewed

    K. Sato, T. Takahashi, Y. Kobayashi, A. Hagino, S. G. Roh, K. Katoh

    DOMESTIC ANIMAL ENDOCRINOLOGY　42　(3)　165-172　2012/04

    DOI： 10.1016/j.domaniend.2011.11.006 　

    ISSN： 0739-7240

  92. 黒毛和種の優良種雄牛クローン子牛の初期成長期における高栄養処理が胸最長筋の遺伝子発現に及ぼす影響 Peer-reviewed

    藤村 亮佑, 藤田 達男, 阿比留 真吾, 衛藤 哲次, 塩塚 雄二, 江原 史雄, 松田 謙一郎, 盧 尚建, 西村 正太郎, 後藤 貴文

    日本畜産学会大会講演要旨集　115回　145-145　2012/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  93. Effects at early stage of life of elevated milk replacer feeding on growth rate, plasma IGF-I concentration and intestinal nutrient transporter expression in Holstein bull calves Peer-reviewed

    Takenori Orihashi, Takanori Mashiko, Kenji Sera, Sang-Gun Roh, Kazuo Katoh, Yoshiaki Obara

    ANIMAL SCIENCE JOURNAL　83　(1)　77-82　2012

    DOI： 10.1111/j.1740-0929.2011.00911.x 　

    ISSN： 1344-3941

  94. Chemerin analog regulates energy metabolism in sheep Peer-reviewed

    Yutaka Suzuki, Sang-Houn Song, Katsuyoshi Sato, Kyoung-Ha So, Astrid Ardiyanti, Shun Kitayama, Yeon-Hee Hong, Sung-Dae Lee, Ki-Choon Choi, Akihiko Hagino, Kazuo Katoh, Sang-Gun Roh

    ANIMAL SCIENCE JOURNAL　83　(3)　263-267　2012

    DOI： 10.1111/j.1740-0929.2011.01002.x 　

    ISSN： 1344-3941

  95. Changes in circulating adiponectin and metabolic hormone concentrations during periparturient and lactation periods in Holstein dairy cows Peer-reviewed

    Yoshihisa Ohtani, Tatsuyuki Takahashi, Katsuyoshi Sato, Astrid Ardiyanti, Sang-Houn Song, Reiichiro Sato, Ken Onda, Yasunori Wada, Yoshiaki Obara, Keiichi Suzuki, Akihiko Hagino, Sang-gun Roh, Kazuo Katoh

    ANIMAL SCIENCE JOURNAL　83　(12)　788-795　2012

    DOI： 10.1111/j.1740-0929.2012.01029.x 　

    ISSN： 1344-3941

  96. Effects of Na-butyrate supplementation in milk formula on plasma concentrations of GH and insulin, and on rumen papilla development in calves Peer-reviewed

    Shin-Ichi Kato, Katsuyoshi Sato, Haruka Chida, Sang-Gun Roh, Shyuichi Ohwada, Shusuke Sato, Paul Guilloteau, Kazuo Katoh

    JOURNAL OF ENDOCRINOLOGY　211　(3)　241-248　2011/12

    DOI： 10.1530/JOE-11-0299 　

    ISSN： 0022-0795

  97. Intervention of the insulino/somatotropic axes in the development of digestive function in pre-weaning calves fed a sodium (Na)-butyrate supplemented diet Peer-reviewed

    Katoh, K, Kato, S, Roh, S.-G, Guilloteau, P

    Advances in Animal Biosciences　2　(2)　469　2011/09/03

  98. 反芻動物における血漿中Apelin 濃度の変動と内分泌調節 Peer-reviewed

    佐藤勝祥, 大川夏貴, 萩野顕彦, 盧尚建, 加藤和雄

    家畜栄養生理研究会会報　55　(1)　17-24　2011/05

    Publisher:

    ISSN： 0286-4754

  99. 体外における未成熟マウス精原肝細胞の分化 Peer-reviewed

    船井 咲知, 高橋 大, 杉本 亮, 盧 尚建, 高木 優二, 鏡味 裕, 小野 珠乙, 佐々木 晋一, 辻井 弘忠, 濱野 光市

    北信越畜産学会報　102　19-25　2011/03

  100. Gene expression and hormonal regulation of adiponectin and its receptors in bovine mammary gland and mammary epithelial cells Peer-reviewed

    Yoshihisa Ohtani, Tomo Yonezawa, Sang-Houn Song, Tatsuyuki Takahashi, Astrid Ardiyanti, Katsuyoshi Sato, Akihiko Hagino, Sang-gun Roh, Kazuo Katoh

    ANIMAL SCIENCE JOURNAL　82　(1)　99-106　2011

    DOI： 10.1111/j.1740-0929.2010.00805.x 　

    ISSN： 1344-3941

  101. Cloning, expression analysis, and regulatory mechanisms of bovine chemerin and chemerin receptor Peer-reviewed

    S. -H. Song, K. Fukui, K. Nakajima, T. Kozakai, S. Sasaki, S. -G. Roh, K. Katoh

    DOMESTIC ANIMAL ENDOCRINOLOGY　39　(2)　97-105　2010/08

    DOI： 10.1016/j.domaniend.2010.02.007 　

    ISSN： 0739-7240

  102. ウシ乳腺におけるアディポネクチンとその受容体の発現と機能に関する研究 Peer-reviewed

    大谷 喜永, 萩野 顕彦, 盧 尚建, 加藤 和雄

    家畜栄養生理研究会会報　54　(1)　1-12　2010/05

    Publisher:

    ISSN： 0286-4754

  103. Prostatic Androgen-Repressed Message-1 as a Regulator of Adipocyte Differentiation in the Mouse Peer-reviewed

    Sang-Houn Song, Yeon-Hee Hong, Shin-ichi Sasaki, Sang-Gun Roh, Kazuo Katoh

    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE　219　(4)　311-317　2009/12

    DOI： 10.1620/tjem.219.311 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  104. 黒毛和牛における枝肉形質、脂肪酸組成、内分泌機能および脂肪合成酵素に関する遺伝子発現に及ぼすGH遺伝子多型の影響 Peer-reviewed

    アルディヤンティ アストリッド, 平山琢二, 阿部剛, 庄司則章, 小林栄治, 千石幸一, 鈴木啓一, 盧尚建, 加藤和雄

    栄養生理研究会報　53　11-18　2009/10

  105. Differential action of trans-10, cis-12 conjugated linoleic acid on adipocyte differentiation of ovine and 3T3-L1 preadipocytes Peer-reviewed

    T. Iga, T. Satoh, S. Yamamoto, K. Fukui, S. H. Song, K. C. Choi, S. G. Roh, S. Sasaki

    Asian-Australasian Journal of Animal Sciences　22　(11)　1566-1573　2009

    Publisher: Asian-Australasian Association of Animal Production Societies

    DOI： 10.5713/ajas.2009.80475 　

    ISSN： 1011-2367

  106. ヤギ精上皮におけるFSHレセプターmRNAの発現 Peer-reviewed

    高橋 大, 船井 咲知, 川西 多恵, 後藤 千寿, 盧 尚建, 小野 珠乙, 佐々木 晋一, 辻井 弘忠, 名倉 義夫, 藤田 優, 濱野 光市

    北信越畜産学会報　94　43-47　2007/11

  107. マウス精子の走温性におけるTransient Receptor Potential Vanilloid (TRPV)-4の関与の可能性 Peer-reviewed

    川西 多恵, 高橋 大, 船井 咲知, 鈴木 誠, 水野 敦子, 後藤 千寿, 盧 尚建, 小野 珠乙, 佐々木 晋一, 辻井 弘忠, 濱野 光市

    北信越畜産学会報　94　35-41　2007/11

  108. Chemerin - A new adipokine that modulates adipogenesis via its own receptor Peer-reviewed

    Sang-Gun Roh, Sang-Houn Song, Ki-Choon Choi, Kazuo Katoh, Valerie Wittamer, Marc Parmentier, Shin-ichi Sasaki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　362　(4)　1013-1018　2007/11

    DOI： 10.1016/j.bbrc.2007.08.104 　

    ISSN： 0006-291X

  109. The regulation of adipogenesis through GPR120 Peer-reviewed

    Chizu Gotoh, Yeon-Hee Hong, Tomoyo Iga, Daisuke Hishikawa, Yasuki Suzuki, Sang-Houn Song, Ki-Choon Choi, Tetsuya Adachi, Akira Hirasawa, Gozoh Tsujimoto, Shin-ichi Sasaki, Sang-Gun Roh

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　354　(2)　591-597　2007/03

    DOI： 10.1016/j.bbrc.2007.01.028 　

    ISSN： 0006-291X

  110. Identification of differentially expressed transcripts in bovine rumen and abomasum using a differential display method Peer-reviewed

    S. G. Roh, M. Kuno, D. Hishikawa, Y. H. Hong, K. Katoh, Y. Obara, H. Hidari, S. Sasaki

    JOURNAL OF ANIMAL SCIENCE　85　(2)　395-403　2007/02

    DOI： 10.2527/jas.2006-234 　

    ISSN： 0021-8812

  111. Molecular cloning and expression analysis of cDNA encoding bovine adipogenin Peer-reviewed

    Yeon-Hee Hong, Yumi Ogihara, Daisuke Hishikawa, Chizu Gotoh, Tomoyo Iga, Yasuki Suzuki, Sang-Houn Song, Keiichi Nakajima, Takaharu Kozakai, Shin-ichi Sasaki, Sang-Gun Roh

    ANIMAL SCIENCE JOURNAL　77　(6)　613-619　2006/12

    DOI： 10.1111/j.1740-0929.2006.00393.x 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  112. Production of lean beef containing a high content of trans-10, cis-12 conjugated linoleic acid by feeding a high-temperature-micro-time-treated diet with extruded soybean Peer-reviewed

    Chengxiong Xu, Honggu Lee, Bokyun Lee, Jihui Wang, Zhongshan Hong, Taegyu Kim, Sangkee Kang, Naggin Choi, Sanggun Roh, Yunjaie Choi

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　70　(11)　2589-2597　2006/11

    DOI： 10.1271/bbb.60037 　

    ISSN： 0916-8451

    eISSN： 1347-6947

  113. Differential regulation of GHRH-receptor and GHS-receptor expression by long-term in vitro treatment of ovine pituitary cells with GHRP-2 and GHRH Peer-reviewed

    Sang-Gun Roh, Maria Doconto, Dan Dan Feng, Chen Chen

    Endocrine　30　(1)　55-62　2006/08

    DOI： 10.1385/ENDO:30:1:55 　

    ISSN： 1355-008X

  114. Differential regulation of GHRH-receptor and GHS-receptor expression by long-term in vitro treatment of ovine pituitary cells with GHRP-2 and GHRH Peer-reviewed

    Sang-Gun Roh, Maria Doconto, Dan Dan Feng, Chen Chen

    ENDOCRINE　30　(1)　55-62　2006/08

    DOI： 10.1385/ENDO:30:1:55 　

    ISSN： 0969-711X

  115. The response of plasma leptin and feed intake to growth hormone administration in Holstein calves with different planes of nutrition Peer-reviewed

    H. G. Lee, Z. S. Hong, M. K. Kim, S. K. Kang, C. X. Xu, J. S. Cho, K. S. Seo, S. G. Roh, Y. J. Choi

    CANADIAN JOURNAL OF ANIMAL SCIENCE　86　(2)　225-233　2006/06

    ISSN： 0008-3984

  116. Reduction in voltage-gated K+ currents in primary cultured rat pancreatic beta-cells by linoleic acids Peer-reviewed

    DD Feng, ZQ Luo, SG Roh, M Hernandez, N Tawadros, DJ Keating, C Chen

    ENDOCRINOLOGY　147　(2)　674-682　2006/02

    DOI： 10.1210/en.2005-0225 　

    ISSN： 0013-7227

  117. Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43 Peer-reviewed

    YH Hong, Y Nishimura, D Hishikawa, H Tsuzuki, H Miyahara, C Gotoh, KC Choi, DD Feng, C Chen, HG Lee, K Katoh, SG Roh, S Sasaki

    ENDOCRINOLOGY　146　(12)　5092-5099　2005/12

    DOI： 10.1210/en.2005-0545 　

    ISSN： 0013-7227

  118. Up-regulation of the claudin-6 gene in adipogenesis Peer-reviewed

    YH Hong, D Hishikawa, H Miyahara, Y Nishimura, H Tsuzuki, C Gotoh, T Iga, Y Suzuki, SH Song, KC Choi, HG Lee, S Sasaki, SG Roh

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　69　(11)　2117-2121　2005/11

    DOI： 10.1271/bbb.69.2117 　

    ISSN： 0916-8451

    eISSN： 1347-6947

  119. Inhibition of growth hormone secretagogue receptor antagonist, [D-Lys-3]-GHRP-6, in adipogenesis of ovine and rat adipocytes Peer-reviewed

    Sang-Gun Roh, Yeon-Hee Hong, Daisuke Hishikawa, Hiroaki Tsuzuki, Hisae Miyahara, Yukihiko Nishimura, Chizu Goto, Yasuki Suzuki, Ki-Choon Choi, Hong-Gu Lee, Shinichi Sasaki

    Animal Science Journal　76　(4)　381-386　2005/08

    DOI： 10.1111/j.1740-0929.2005.00279.x 　

    ISSN： 1344-3941

  120. Up-regulation of adipogenin, an adipocyte plasma transmembrane protein, during adipogenesis Peer-reviewed

    YH Hong, D Hishikawa, H Miyahara, H Tsuzuki, Y Nishimura, C Gotoh, KC Choi, Y Hokari, Y Takagi, HG Lee, KK Cho, SG Roh, S Sasaki

    MOLECULAR AND CELLULAR BIOCHEMISTRY　276　(1-2)　133-141　2005/08

    DOI： 10.1007/s11010-005-3673-0 　

    ISSN： 0300-8177

  121. Identification of genes expressed differentially in subcutaneous and visceral fat of cattle, pig, and mouse Invited Peer-reviewed

    D Hishikawa, YH Hong, S Roh, H Miyahara, Y Nishimura, A Tomimatsu, H Tsuzuki, C Gotoh, M Kuno, KC Choi, H Lee, KK Cho, H Hidari, S Sasaki

    PHYSIOLOGICAL GENOMICS　21　(3)　343-350　2005/05

    DOI： 10.1152/physiolgenomics.00184.2004 　

    ISSN： 1094-8341

  122. Differential expression of the nonmuscle-type cofilin gene between subcutaneous and visceral adipose tissue Peer-reviewed

    KC Choi, SG Roh, D Hishikawa, H Miyahara, M Kuno, H Tsuzuki, A Tomimatsu, YH Hong, KK Cho, KH Han, S Sasaki

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　67　(10)　2262-2265　2003/10

    DOI： 10.1271/bbb.67.2262 　

    ISSN： 0916-8451

    eISSN： 1347-6947

  123. The role of phosphatidylinositol 3-kinase and mitogenic activated protein kinase on the differentiation of ovine preadipocytes Peer-reviewed

    KC Choi, YB Shrestha, SG Roh, D Hishikawa, M Kuno, H Tsuzuki, YH Hong, S Sasaki

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　16　(8)　1199-1204　2003/08

    ISSN： 1011-2367

  124. The role of ghrelin and growth hormone secretagroogues receptor on rat adipogenesis Peer-reviewed

    KC Choi, SG Roh, YH Hong, YB Shrestha, D Hishikawa, C Chen, M Kojima, K Kangawa, SI Sasaki

    ENDOCRINOLOGY　144　(3)　754-759　2003/03

    DOI： 10.1210/en.2002-220783 　

    ISSN： 0013-7227

  125. Orexin-B augments voltage-gated L-type Ca2+ current via protein kinase C-mediated signalling pathway in ovine somatotropes Peer-reviewed

    RW Xu, SG Roh, CH Gong, M Hernandez, Y Ueta, C Chen

    NEUROENDOCRINOLOGY　77　(3)　141-152　2003/03

    DOI： 10.1159/000069507 　

    ISSN： 0028-3835

  126. めん羊前駆および成熟脂肪細胞の増殖・分化制御機構 Peer-reviewed

    佐々木 晋一, 盧 尚建, 崔 基春

    家畜栄養生理研究会会報, , - ()　46　(2)　101-119　2002/11

    Publisher:

    ISSN： 0286-4754

  127. Increase in expression of growth hormone secretagouges-receptor on differentiation of ovine preadipocytes Peer-reviewed

    Roh S.G, es, onding autho, K.C. Choi, Y. B. Shrestha, C. Yoon, J.H. Woo, Sasaki S

    Anim. Sci. J., , - ()　73　305-308　2002/08

    DOI： 10.1046/j.1344-3941.2002.00043.x 　

  128. Is GHRH receptor essential to GHRP-2-induced GH secretion in primary cultured rat pituitary cells? Peer-reviewed

    SG Roh, C Chen, KC Choi, Y Shrestha, SI Sasaki

    ENDOCRINOLOGY　143　(5)　1964-1967　2002/05

    DOI： 10.1210/en.143.5.1964 　

    ISSN： 0013-7227

  129. Direct modification of somatotrope function by long-term leptin treatment of primary cultured ovine pituitary cells Peer-reviewed

    SG Roh, GY Nie, K Loneragan, A Gertler, C Chen

    ENDOCRINOLOGY　142　(12)　5167-5171　2001/12

    DOI： 10.1210/en.142.12.5167 　

    ISSN： 0013-7227

  130. The role of GH-releasing hormone receptor and GH-releasing peptide receptor on GH secretion in ruminant Peer-reviewed

    Roh S.G, S. Sasaki, H. Hidari, C. Chen

    家畜栄養生理研究会会報　45　39-50　2001/05

  131. The in vitro effect of leptin on growth hormone secretion from primary cultured ovine somatotrophs Peer-reviewed

    C Chen, SG Roh, GY Nie, K Loneragan, RW Xu, M Ruan, IJ Clarke, JW Goding, A Gertler

    ENDOCRINE　14　(1)　73-78　2001/02

    ISSN： 0969-711X

  132. Differential signaling pathway on antilipolytic action of insulin in sheep and rat adipocytes Peer-reviewed

    S Sasaki, SG Roh, K Sawada

    PERSPECTIVE IN COMPARATIVE ENDOCRINOLOGY: UNITY AND DIVERSITY　1273-1278　2001

  133. Diverse intracellular signalling systems used by growth hormone-releasing hormone in regulating voltage-gated Ca2+ or K+ channels in pituitary somatotropes Invited Peer-reviewed

    C Chen, RW Xu, IJ Clarke, M Ruan, K Loneragan, SG Roh

    IMMUNOLOGY AND CELL BIOLOGY　78　(4)　356-368　2000/08

    DOI： 10.1046/j.1440-1711.2000.00917.x 　

    ISSN： 0818-9641

  134. Human GHRH reduces voltage-gated K(+) currents via a non-cAMP-dependent but PKC-mediated pathway in human GH adenoma cells Peer-reviewed

    RW Xu, SG Roh, K Loneragan, M Pullar, C Chen

    JOURNAL OF PHYSIOLOGY-LONDON　520　(3)　697-707　1999/11

    DOI： 10.1111/j.1469-7793.1999.00697.x 　

    ISSN： 0022-3751

  135. The in vitro effect of leptin on basal and growth hormone-releasing hormone-stimulated growth hormone secretion from the ovine pituitary gland Peer-reviewed

    SG Roh, IJ Clarke, RW Xu, JW Goding, K Loneragan, C Chen

    NEUROENDOCRINOLOGY　68　(6)　361-364　1998/12

    ISSN： 0028-3835

  136. The relationship between lipogenic activity and the size of adipocytes from subcutaneous adipose tissue of Holstein steers during the fattening period Peer-reviewed

    He M.L, S.G. Roh, E. Shibasaki, S. Hidaka, N. Matsunaga, H. Hidari

    Animal Science and Technology　69　(5)　424-432　1998/05

    Publisher: Japanese Society of Animal Science

    DOI： 10.2508/chikusan.69.424 　

    ISSN： 1346-907X

    More details Close

    Isolated adipocytes were used to evaluate the lipogenic activity in subcutaneous adipose tissue of Holstein steers during various fattening periods. During the fattening period, both the mean diameter of adipocytes and the lipogenesis increased continually. There was a positive correlation between the mean diameter of adipocytes and the lipogenesis from glucose (r =0.74, P<0.05) or from acetate (r=0.81, P<0.05). Further, the relationship between the size of adipocyte and the lipogenic activity was investigated. It can be done through measuring the incorporation rate of substrates into lipids in each fraction of adipocytes which were separated by meshes. It was shown that both the incorporation rate of glucose or acetate into lipids and the relative contribution ratio of incorporation rate of acetate to glucose in the adipocytes taken from finishing steers, were significantly larger (P<0.05) than those from the early fattening steers. In the adipocytes which were taken from early fattening steers, the incorporation rate of glucose or acetate into lipids became larger with increment of the mean diameter from fraction 1 (33μm) to fraction 6 (139μm) (both: r=0.72, P<0.05). In the adipocytes which were taken from finishing fattening steers, the incorporation rate of glucose into lipids increased with the increment of the mean diameter from fraction 1 (36μm) to fraction 6 (164μm) (r=0.79, P<0.05), while the incorporation rate of acetate into lipids increased only with increment of the mean diameter from fraction 1 (36μm) to fraction 4 (116μm), then decreased with the increment of the mean diameter from fraction 4( 116μm) to those very large adipocytes in fraction 6 (164μm).

  137. Effect of cholinergic blockade on inhibited GH secretion by feeding and intraruminal VFA infusion in sheep Peer-reviewed

    Matsunaga N, M. Wakiya, S. G. Roh, M. Hirota, M. L. He, S. Hidaka, H. Hidari

    Am. J. Physiol. Endocrinol. Metab.　274　E45-E51　1998/02

  138. No desensitization of the growth hormone (GH) response between GH-releasing peptide-2 and GH-releasing factor in calves Peer-reviewed

    SG Roh, ML He, N Matsunaga, S Hidaka, H Hidari

    JOURNAL OF ANIMAL SCIENCE　75　(10)　2749-2753　1997/10

    ISSN： 0021-8812

  139. Effect of mesenteric venous volatile fatty acids (VFA) infusion on GH secretion in sheep Peer-reviewed

    N Matsunaga, Kubota, I, SG Roh, ML He, S Hidaka, H Hidari, Y Sasaki

    ENDOCRINE JOURNAL　44　(5)　707-714　1997/10

    DOI： 10.1507/endocrj.44.707 　

    ISSN： 0918-8959

  140. Mechanisms of action of growth hormone-releasing peptide-2 in bovine pituitary cells Peer-reviewed

    SG Roh, ML He, N Matsunaga, S Hidaka, H Hidari

    JOURNAL OF ANIMAL SCIENCE　75　(10)　2744-2748　1997/10

    ISSN： 0021-8812

  141. Relationship between the fatty acid composition and the size of adipocytes from subcutaneous adipose tissue of Holstein steers during the fattening period Peer-reviewed

    He M.L, S. G. Roh, H. Oka, S. Hidaka, N. Matsunaga, H. Hidari

    Animal Science and Technology　68　(9)　838-842　1997/09

    Publisher: Japanese Society of Animal Science

    DOI： 10.2508/chikusan.68.838 　

    ISSN： 1346-907X

    More details Close

    The fatty acid composition and the diameter of subcutaneous adipocytes from 13 Holstein steers at different fattening stages were investigated. The mean diameter of adipocytes from individual steers was positively (P<0.01) correlated with the percentage of myristoleic acid (14:1)(r=0.91), palmitoleic acid (16:1)(r=0.83) or oleic acid (18:1)(r=0.82), and negatively(P<0.01)correlated with the percentage of stearic acid (18:0)(r=-0.89). The isolated adipocytes from the same finishing steer (n=5)were then separated by a 50μm mesh. The large adipocytes which remained on the mesh (118μm mean diameter)contained more (P<0.01) myristoleic acid (14:1)palmitoleic acid (16:1) and oleic acid (18:1), and less (P<0.01) palmitic acid(16:0), stearic acid (18:0) than the small adipocytes which were filtered through (29μm mean diameter). It is suggested that the growth of adipocyte is one of the important determinants of fatty acid composition in subcutaneous adipocytes of Holstein steers throughout the fattening period.

  142. Effect of different experimental conditions on lipogenesis and substrate oxidation in isolated adipocytes from fattening Holstein steers Peer-reviewed

    ML He, S Hidaka, N Matsunaga, SG Roh, H Hidari

    REPRODUCTION NUTRITION DEVELOPMENT　37　(5)　529-539　1997/09

    DOI： 10.1051/rnd:19970504 　

    ISSN： 0926-5287

  143. Competitive enzyme immunoassay for bovine growth hormone Peer-reviewed

    SG Roh, N Matsunaga, A Miyamoto, S Hidaka, H Hidari

    ENDOCRINE JOURNAL　44　(1)　195-198　1997/02

    DOI： 10.1507/endocrj.44.195 　

    ISSN： 0918-8959

  144. Effects of muscarinic and adrenergic blockade on growth hormone secretion induced by growth hormone-releasing peptide-2 (GHRP-2) in ovariectomized ewes Peer-reviewed

    Sang-Gun Roh, Minoru Hirota, Mao Long He, Nobuyoshi Matsunaga, Satoshi Hidaka, Hisashi Hidari

    Endocrine Journal　44　(6)　861-865　1997

    Publisher: Japan Endocrine Society

    DOI： 10.1507/endocrj.44.861 　

    ISSN： 0918-8959

  145. Effects of feed reduction on plasma growth hormone and insulin-like growth factor-1 concentrations and the growth hormone response to growth hormone releasing factor in calves Peer-reviewed

    Hidari H, S. Hidaka, T. Ishida, N. Matsunaga, S.G. Roh

    J. Reprod. Dev., , - ()　42　132-134　1996/11

  146. Characteristics of growth hormone secretion responsiveness to growth hormone-releasing peptide-2 (GHRP-2 or KP102) in calves Peer-reviewed

    Sang-Gun Roh, Nobuyoshi Matsunaga, Satoshi Hidaka, Hisashi Hidari

    Endocrine Journal　43　(3)　291-298　1996

    Publisher: Japan Endocrine Society

    DOI： 10.1507/endocrj.43.291 　

    ISSN： 0918-8959

  147. NONHORMONAL FACTORS ON DIFFERENTIATION OF BOVINE MAMMARY CELLS Peer-reviewed

    SG ROH, JH WOO, MG BAIK, YJ CHOI

    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL　30A　(5)　292-294　1994/05

    ISSN： 1071-2690

    eISSN： 1543-706X

  148. EFFECT OF LACTOGENIC HORMONES ON PROTEIN-SYNTHESIS AND AMINO-ACID-UPTAKE IN RAT MAMMARY ACINAR CELL-CULTURE AT VARIOUS PHYSIOLOGICAL STAGES Peer-reviewed

    SG ROH, MG BAIK, YJ CHOI

    INTERNATIONAL JOURNAL OF BIOCHEMISTRY　26　(4)　479-485　1994/04

    DOI： 10.1016/0020-711X(94)90003-5 　

    ISSN： 0020-711X

Show all ︎Show first 5

Misc. 58

  1. 黒毛和種におけるメタン産生関連形質および生理指標に及ぼす候補種雄牛の影響

    金民知, 正木達規, 及川康平, 岩本英治, 生田健太郎, 芦原茜, 盧尚建, 寺田文典, 野中最子

    日本畜産学会大会講演要旨　131st　2023

    ISSN： 1342-4688

  2. 黒毛和種牛の肥育期における血中タウリン濃度の動態とその栄養生理的な特徴

    高井俊太朗, 鋪田莉佳, 正木達規, 生田健太郎, 岩本英治, LEE Honggu, 鳥居伸一郎, 芦原茜, 金民知, 寺田文典, 芳賀聡, 盧尚建

    日本畜産学会大会講演要旨　131st　2023

    ISSN： 1342-4688

  3. 黒毛和種メタン産生高低牛の栄養生理的な特徴

    金民知, 平井洵, 正木達規, 生田健太郎, 岩本英治, 上本吉伸, 寺田文典, 盧尚建

    日本畜産学会大会講演要旨　129th　2021

    ISSN： 1342-4688

  4. The effect of chemerin as a host-derived factor on intestinal microbial activity in calves

    Yutaka Suzuki, Kanako Kubota, Hiroto Miura, Satoshi Haga, Sanggun Roh, Satoshi Koike, Yasuo Kobayashi

    135　2018/07

  5. CCL5–monocyte/macrophage axis maintains homeostasis in rumen epithelium

    Koki Nishihara, Sanggun Roh, Satoshi Haga

    2018 ASAS-CSAS Annual Meeting & Trade Show　PSXI-17　2018/07

  6. ウシ第１胃（ルーメン）における生体防御とケモカインの関係

    西原昂来, 鈴木裕, 金多慧, 芳賀聡, 盧尚建

    2017年度生命科学系学会合同年次大会（ConBio2017）要旨集　2P-1287　2017/12

  7. 初乳合成期のウシ乳腺におけるGlucose・BHBA取込率および特異輸送体遺伝子発現の時系列的変化

    芳賀聡, 中野美和, 宮地慎, 松山裕城, 石崎宏, 加藤和雄, 盧尚建

    第48回乳腺・泌乳研究会研究集会講演要旨集　6　2017/12

  8. Rumen epithelial cells as a source of inflammatory cytokines

    K. Nishihara, Y. Suzuki, S. Haga, D.H. Kim, S.G. Roh

    50　2017/10

  9. Sensory Study of the Effect of Seaweed Addition on Beef

    60　(2)　129-133　2017/09

    Publisher: 日本暖地畜産学会

    ISSN： 2185-081X

  10. 哺乳仔ウシの内分泌、免疫機能および発育性に及ぼすリゾープス麹抽出物添加代用乳給餌の影響

    矢島優, 西原昂来, 金多慧, 芳賀聡, 中野美和, 石崎宏, 牛越康一郎, 鈴木武人, 鈴木啓一, 加藤和雄, 盧尚建

    日本畜産学会第122回大会講演要旨　122回　178-178　2017/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  11. ウシルーメン絨毛組織の発達におけるIGFとIGFBPsの役割

    西原昂来, 鈴木裕, 金多慧, 中野美智, 中野美和, 石崎宏, 芳賀聡, 盧尚建

    日本畜産学会第122回大会講演要旨　122回　109-109　2017/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  12. 牛肉への海藻類浸漬が食味官能評価に与える影響

    山中麻帆, 平山琢二, 林英明, 盧尚建, 加藤和雄, 鈴木啓一, 及川卓郎

    日本畜産学会大会講演要旨　123rd　2017

    ISSN： 1342-4688

  13. Changes of the hepatic expression of α-tocopherol-related genes in relation to the hypovitaminosis E around calving in dairy cows Peer-reviewed

    Satoshi HAGA, Miwa Nakano, Makoto Miyaji, Hiroshi Ishizaki, Sang-gun Roh

    2016 17th AAAP Animal Science Congress　377　2016/08

  14. Gene expression and secretion of chemerin in bovine mammary epithelial cells. Peer-reviewed

    Suzuki Yutaka, Chiba Sachi, Haga Satoshi, Roh Sanggun

    144　2016/07

  15. 離乳前後の仔牛の肝臓組織における網羅的トランスクリプトーム解析

    盧尚建, 近藤光彦, 鈴木裕, 加藤大地, 芳賀聡, 川原玲香, 河野友宏, 鈴木啓一, 加藤和雄

    日本畜産学会第121回大会講演要旨　198　2016/03

  16. 黒毛和種牛のルーメン発達関連因子の解析

    加藤大地, 鈴木裕, 芳賀聡, 蘇敬夏, 川原玲香, 河野友宏, 加藤和雄, 盧尚建

    日本畜産学会第121回大会講演要旨　243　2016/03

  17. 「妊娠有無」を乾乳牛の乳腺機能変化の要因として考慮すべきか？

    芳賀聡, 中野美和, 宮地慎, 石崎宏, 松山裕城, 米倉真一, 盧尚建

    日本畜産学会第121回大会講演要旨　243　243-243　2016/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  18. Verification of index related to delicious of Japanese Black Cattle Beef meat based on metabolomics analytical result

    鈴木啓一, 加藤和雄, HONG Yu, 鈴木裕, ROH Sanggun, 飯田文子, 小松智彦

    食肉に関する助成研究調査成果報告書　34　2016

  19. 反芻動物の生産に関する最近の研究動向：第4章 泌乳生理機能と乳量制御

    芳賀 聡, 鈴木 裕, 加藤大地, 加藤 和雄, 盧 尚建

    畜産の研究　69　(12)　1005-1009　2015/12

  20. 反芻動物の生産に関する最近の研究動向：第3章 内分泌調節機能としての肝臓の機能

    鈴木 裕, 加藤大地, 加芳賀 聡, 藤 和雄, 盧 尚建

    畜産の研究　69　(11)　929-934　2015/11

  21. 反芻動物の生産に関する最近の研究動向：第2章 反芻動物のルーメン発達と生産性との関連

    加藤大地, 鈴木 裕, 芳賀 聡, 加藤 和雄, 盧 尚建

    畜産の研究　69　(10)　861-866　2015/10

  22. 周産期の肝臓組織におけるANGPTL8の発現調節

    中野美智, 鈴木裕, 芳賀聡, 中野美和, 宮地慎, 石崎宏, 蘇敬夏, 加藤和雄, 盧尚建

    日本畜産学会第120回大会講演要旨　120回　90-90　2015/09

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  23. 泌乳期と乾乳期の乳牛における内分泌因子ケメリンのシグナル調節とその役割

    鈴木裕, 中島恵一, 芳賀聡, 中野美和, 宮地慎, 石崎宏, 盧尚建

    日本畜産学会第120回大会講演要旨　120回　42-42　2015/09

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  24. 初乳生産期の乳腺におけるGlucose・BHBA取込率および輸送体発現

    芳賀聡, 中野美和, 宮地慎, 松山裕城, 加藤和雄, 盧尚建

    日本畜産学会第120回大会講演要旨　87　2015/09

  25. 反芻動物の生産に関する最近の研究動向：第1章 短鎖脂肪酸の生理機構

    加藤和雄, 鈴木啓一, 盧尚建

    畜産の研究　69　(9)　773-782　2015/09

    Publisher: 養賢堂

    ISSN： 0009-3874

  26. Expressional regulation of chemerin and its receptors in the liver and adipose tissues of young cattle by weaning and nutrition. Peer-reviewed

    ADSA-ASAS JAM 2015　588　2015/07

  27. RNA-Seqによる黒毛和種牛ルーメン発達関連遺伝子の網羅的トランスクリプトーム解析

    加藤 大地, 鈴木 裕, 芳賀 聡, 蘇 敬夏, 川原 玲香, 河野 友宏, 加藤 和雄, 盧 尚建

    日本畜産学会大会講演要旨集　119回　186-186　2015/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  28. ウシBetatrophin の遺伝子発現調節について

    盧尚建, 山内恵利, 鈴木裕, 加藤大地, 近藤光彦, 畑中陽弦, 芳賀聡, 蘇敬夏, SeungJu Park, Myunggi Baik, 加藤和雄

    日本畜産学会第119回大会講演要旨　184　2015/03

  29. ウシ乳腺上皮細胞におけるケメリンの糖・脂質代謝関連遺伝子発現の調節作用

    加藤大幾, 鈴木裕, 芳賀聡, 蘇敬夏・Lee Hong-Gu・Jung U-Suk, 加藤和雄, 盧尚建

    日本畜産学会第119回大会講演要旨　184　2015/03

  30. ウシ培養肝細胞におけるケメリン調節因子に関する研究

    近藤光彦, 鈴木裕, 芳賀聡, 畑中陽弦, 加藤大地, 蘇敬夏, 加藤和雄, 盧尚建

    日本畜産学会第119回大会講演要旨　184　2015/03

  31. Tissues distribution and expression changes of the α-tocopherol related genes due to short term high dose oral administration of α-tocopherol in weaned beef calves Peer-reviewed

    芳賀聡, 中野美和, 石崎宏, 盧尚建, 加藤和雄

    Joint ISNH/ISRP International conference 2014　30　142　2014/09

  32. Gene expression and regulation of betatrophin in cultured hepatocytes of preweaning and postweaning calves Peer-reviewed

    Joint ISNH/ISRP International conference 2014　30　306　2014/09

  33. ウシ乳腺上皮細胞におけるαトコフェロール細胞内輸送関連分子の発現に及ぼす催乳性ホルモンの影響

    芳賀聡, 小林洋介, 中野美和, 石崎宏, 盧尚建, 加藤和雄

    日本畜産学会第118回大会講演要旨　118回　148-148　2014/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  34. ウシBetatropinの発現と調節

    山内恵利, 鈴木裕, 東北, 蘇敬夏, 東北, 農, 芳賀聡, 加藤和雄, 盧尚建, 東北大院農

    日本畜産学会第118回大会講演要旨　231　2014/03

  35. MPS肺病変選抜純粋種と末梢血免疫能選抜純粋種を交雑した三元豚の抗病性及び免疫能力の比較

    陸拾七, 島津朋之, 片山雄貴, 大津良輔, 米野智之, 内田隆史, 北澤春樹, 麻生久, 盧尚建, 加藤和雄, 須田義人, 佐久間晶子, 中條満, 鈴木啓一

    日本養豚学会大会講演要旨　100th　2014

  36. 頭脳循環を加速する若手研究者戦略的海外派遣プログラムー(3)ドイツライプニッツ家畜生物学研究所およびミュンヘン工科大学を訪問して

    後藤貴文, 中村真子, 柴田昌宏, 盧尚建, 辰巳隆一, 古瀬充宏

    畜産の研究　67　(9)　907-912　2013/09

  37. ニューロテンシンによる精子受精能力促進作用と雌性生殖器官における発現

    平舘 裕希, 井上 弘貴, 小林 記緒, 白形 芳樹, 盧 尚建, 加藤 和雄, 佐藤 英明, 種村 健太郎

    日本獣医学会学術集会講演要旨集　156回　198-198　2013/08

    Publisher: (公社)日本獣医学会

    ISSN： 1347-8621

  38. Effect of propionate, palmitate and insulin on chemerin gene expression in monolayer cultures of bovine hepatocytes. Peer-reviewed

    T340　2013/07

  39. Hormonal regulation of α-tocopherol transfer related molecules expression in bovine mammary epithelial cells. Peer-reviewed

    Haga Satoshi

    ADSA-ASAS JAM2013　TH283　2013/07

  40. 頭脳循環を加速する若手研究者戦略的海外派遣プログラムー(2)タイチェンマイ大学を訪問して

    後藤貴文, 中村真子, 柴田昌宏, 盧尚建, 辰巳隆一, 古瀬充宏

    畜産の研究　67　(5)　559-566　2013/05

    Publisher: 養賢堂

    ISSN： 0009-3874

  41. The changes of chemerin and chemerin receptor to regulate lipid metabolism in liver and pituitary gland

    Sanggun Roh, Shun Kitayama, Astrid Ardiyanti, Yutaka Suzuki, Eri Yamauchi, Daiki Kato, Kwonjung Yi, Kyoungha So, Akihiko Hagino, Kazuo Katoh

    FASEB JOURNAL　27　2013/04

    ISSN： 0892-6638

  42. 子牛における血中オキシトシン濃度とストレス反応に及ぼす自然哺乳およびブラッシングの効果(日本家畜管理学会・応用動物行動学会2013年度春季合同研究発表会)

    陳 絲宇, 田中 繁史, 有野 祐樹, 盧 尚建, 佐藤 衆介

    日本家畜管理学会誌・応用動物行動学会誌　49　(1)　43　2013

    Publisher: 動物の行動と管理学会

    DOI： 10.20652/abm.49.1_43 　

    ISSN： 1880-2133

    eISSN： 2424-1776

  43. 仔牛のサイエンス（２）

    加藤和雄, 萩野顕彦, 増子孝則, 折橋毅典, 小原嘉昭, 盧 尚建

    畜産の研究　66　(5)　531-537　2012/05

    Publisher: 養賢堂

    ISSN： 0009-3874

  44. 頭脳循環を加速する若手研究者戦略的海外派遣プログラムー(1)ドイツライプニッツ家畜生物学研究所を訪問して

    後藤貴文, 中村真子, 柴田昌宏, 盧尚建, 辰巳隆一, 古瀬充宏

    畜産の研究　66　(5)　501-510　2012/05

  45. Deletion of adipogenin gene in mice is effected to adipogenesis and deferred to growth

    So Kyoung-Ha, Taisei Tachizaki, Astrid Aridiyanti, Yutaka Suzuki, Sang-Houn Song, Sang-gun Roh, Kazuo Katoh

    FASEB JOURNAL　26　2012/04

    ISSN： 0892-6638

  46. 仔牛のサイエンス（１）

    加藤和雄, 萩野顕彦, 増子孝則, 折橋毅典, 小原嘉昭, 盧 尚建

    畜産の研究　66　(4)　429-434　2012/04

    Publisher: 養賢堂

    ISSN： 0009-3874

  47. 子牛における血中オキシトシンの個体差とストレス反応性との関係(日本家畜管理学会・応用動物行動学会合同2012年度春季研究発表会)

    陳 絲宇, 田中 繁史, 有野 祐樹, 千葉 純子, 佐藤 和也, 千葉 孝, 親川 千紗子, 盧 尚建, 佐藤 衆介

    日本家畜管理学会誌・応用動物行動学会誌　48　(1)　27　2012

    Publisher: 動物の行動と管理学会

    DOI： 10.20652/abm.48.1_27 　

    ISSN： 1880-2133

    eISSN： 2424-1776

  48. Pilot survey of feral cattle in the vicinity of Fukushima nuclear power planty

    Wada M, H. Haba, S. Sato, S. Roh, M. Toyomizu

    RIKEN Accel. Prog. Rep　45　248　2012

  49. 頭脳循環を加速する若手研究者戦略的海外派遣プログラム ―(1)ドイツライプニッツ家畜生物学研究所を訪問して

    後藤貴文, 中村真子, 辰巳隆一, 古瀬充宏, 柴田昌宏, 盧 尚建

    畜産の研究（養賢堂）　66　(5)　505-509　2012

  50. 国際反芻動物生理学シンポジウム（ISRP2009）第11回大会参加報告

    加藤和雄, 佐藤勝祥, 宋相憲, 大谷喜永, 萩野顕彦, 盧尚建

    畜産の研究　64　729-735　2010/10

  51. Possible functional roles of chemerin, a new adipokine, in domestic animals

    Sang-Gun Roh, Sang-Houn Song, Yutaka Suzuki, Kazuo Katoh

    FASEB JOURNAL　24　2010/04

    ISSN： 0892-6638

  52. Overexpression of adipogenin enhances the fat accumulation of C2C12 myoblasts

    Sang-Houn Song, Sang-Gun Roh, Kazuo Katoh

    FASEB JOURNAL　23　2009/04

    ISSN： 0892-6638

  53. UP-REGULATION OF CHEMERIN AND CHEMERIN RECEPTOR BY TNF-alpha IN BOVINE DIFFERENTIATED ADIPOCYTES IN VITRO

    Sang-Gun Roh, Sang-Houn Song, Kazuo Katoh

    JOURNAL OF PHYSIOLOGICAL SCIENCES　59　413-413　2009

    ISSN： 1880-6546

  54. Cloning and expression pattern of bovine adipogenin isoform

    S. G. Roh, T. Satoh, S. Shinichi

    JOURNAL OF ANIMAL SCIENCE　85　295-295　2007

    ISSN： 0021-8812

  55. Expression and functional study of proteins involved in the accumulation of intramuscular fat within the bovine longissimus dorsi muscle

    H. G. Lee, J. A. Han, C. Xu, S. H. Kim, K. S. Seo, D. H. Yoon, S. G. Roh, Y. J. Choi

    MOLECULAR & CELLULAR PROTEOMICS　5　(10)　S318-S318　2006/10

    ISSN： 1535-9476

  56. Control of adipogenesis in ruminants

    Sang-Gun Roh, Daisuke Hishikawa, Yeon-Hee Hong, Shinichi Sasaki

    ANIMAL SCIENCE JOURNAL　77　(5)　472-477　2006/10

    DOI： 10.1111/j.1740-0929.2006.00374.x 　

    ISSN： 1344-3941

  57. Characterization of growth hormone secretion to growth hormone-releasing peptide-2 in domestic animals - A review

    SG Roh, HG Lee, LT Phung, H Hidari

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES　15　(5)　757-766　2002/05

    ISSN： 1011-2367

  58. Leptin modulates the synthesis of growth hormone (GH) and receptors of GH-releasing hormone (GHRH-R) and GH-releasing peptides (GHRP-R) in primary cultured ovine somatotropes

    SG Roh, C Chen

    JOURNAL OF NEUROCHEMISTRY　78　75-75　2001/09

    ISSN： 0022-3042

Show all ︎Show first 5

Books and Other Publications 4

  1. ルーメンの科学

    小林, 泰男, 板橋, 久雄

    農山漁村文化協会　2023/05

    ISBN: 9784540231209

  2. 農学生命科学を学ぶための入門生物学

    鳥山欽哉

    東北大学出版会　2020

  3. 新編 家畜生理学

    加藤 和雄, 古瀬 充宏, 盧 尚建

    2015/04/01

  4. Differential signaling pathway on antilipolytic action of insulin in sheep and rat adipocyte, Prospect in Comparative Endocrinology: Unity and Diversity.

    Sasaki S, S.G. Roh, Sawada K

    2001/07

Presentations 112

  1. The Importance of Hepatokines in Metabolic Regulation of Ruminants International-presentation

    Sanggun Roh, Misato Nakano, Koki Nishihara, Dahye Kim, Yutaka Suzuki

    2016 International Animal Sciences and Biotechnology Forum　2016/12/07

  2. Comparison of the Gene Expression of Rumen Epithelium in Pre- and Post-Weaning Young Cattle International-presentation

    Sanggun ROH, Yutaka SUZUKI, Daichi KATO, Satoshi HAGA

    17th AAAP ANIMAL SCIENCE CONGRESS　2016/08/22

  3. Gene expression and secretion of chemerin in bovine mammary epithelial cells International-presentation

    Yutaka Suzuki, Sachi Chiba, Satoshi Haga, Sanggun Roh

    2016 ADSA®-ASAS Joint Annual Meeting Abstract Presentation Notice　2016/07/19

  4. 離乳前後の仔牛の肝臓組織における網羅的トランスクリプトーム解析

    加藤 大地, 鈴木 裕, 芳賀 聡, 蘇 敬夏, 川原 玲香, 河野 友宏, 加藤 和雄, 盧 尚建

    日本畜産学会大121回大会　2016/03/26

  5. 離乳前後の仔牛の肝臓組織における網羅的トランスクリプトーム解析

    盧 尚建, 近藤 光彦, 鈴木 裕, 加藤 大地, 芳賀 聡, 川原 玲香, 河野 友宏, 鈴木 啓一, 加藤 和雄

    日本畜産学会大 121回大会　2016/03/26

  6. 妊娠の有無がホルスタイン経産乾乳牛の乳腺組織機能遺伝子発現に及ぼす影響

    芳賀聡, 中野美和, 宮地慎, 石崎宏, 松山裕城, 米倉真一, 盧尚建

    日本畜産学会大 121回大会　2016/03/26

  7. 培養脂肪細胞におけるヨード卵の卵黄の脂肪蓄積抑制の効果

    横山 次郎, 森岡 恵美, 鈴木 啓一, 加藤 和雄, 盧 尚建

    日本畜産学会大 121回大会　2016/03/26

  8. GH多型による黒毛和種牛の脂肪細胞の生理的特徴

    鈴木裕, 谷田奈々子, 加藤大地, 村井篤嗣, 平山琢二, 加藤和雄, 盧尚建

    日本畜産学会大 121回大会　2016/03/26

  9. Current situation of Japanese Black cattle. International-presentation

    Sang-Gun Roh

    The 2015 International Conference on Beef Cattle Improvement and Industrialization　2015/11/16

  10. Current situation of Japanese Black cattle. International-presentation

    Sang-Gun Roh

    1st International Beef cattle symposium　2015/10/09

  11. Gene expression and regulation of betatrophin in cultured hepatocytes of preweaning and postweaning calves International-presentation

    Roh SG, Yamauchi E, Suzuki Y, Kitayama S, Kondo M, Nakano M, Ishizaki H, Haga S, Katoh K

    Joint ISNH/ISRP International Conference 2014　2014/09/08

  12. Tissues distribution and expression changes of the α-tocopherol related genes due to short term high dose oral administration of α-tocopherol in weaned beef calves International-presentation

    Haga S, Nakano M, Ishizaki H, Roh SG, Katoh K

    Joint ISNH/ISRP International Conference 2014　2014/09/08

  13. Effects of antioxidants on hydrogen peroxide-induced cell death in bovine mammary epithelial cells. International-presentation

    Hagino A, Kishita S, Nicholas K, Roh SG, Katoh K

    Joint ISNH/ISRP International Conference 2014　2014/09/08

  14. Postprandial reduction in plasma GH concentration is caused by insulin in goats International-presentation

    Kobayashi R, Harjanti DW, Sano H, Roh SG, Katoh K

    Joint ISNH/ISRP International Conference 2014　2014/09/08

  15. Chemerin mRNA expression is regulated by insulin and fatty acids in the liver of calves International-presentation

    Suzuki Y, Kitayama S, Kondo M, Haga S, Katoh K, Roh SG

    Joint ISNH/ISRP International Conference 2014　2014/09/08

  16. Adipokines vs Hepatokines in ruminants International-presentation

    Sang-Gun Roh

    The 3rd International Symposium on New Challenge for Animal Science Research　2014/07/03

  17. The importance of oxytocin receptor in adipocytes International-presentation

    Roh SG, Katoh K

    The 2nd International Symposium on New Challenge for Animal Science Research　2013/11/16

  18. Adipogenin, a regulatory gene plays role in fat accumulation of adipogenesis International-presentation

    Kyoung-ha So, Sanghoun Song, Yuki Hata, Yuki Gumagai, Yutaka Suzuki, Kazuo Katoh, Sang-Gun Roh

    4th International Congress on Abdominal Obesity　2013/09/12

  19. Deficiency of adipogenin decreases fat accumulation and alters glucose metabolism International-presentation

    Yuki Hata, Kyoung-ha So, Yuki Gumagai, Yutaka Suzuki, Kazuo Katoh, Sang-Gun Roh

    4th International Congress on Abdominal Obesity　2013/09/12

  20. 黒毛和種牛の内分泌変化と糖代謝に及ぼすGH多型の影響

    小林諒子, 盧尚建, 佐野 宏明, 加藤和雄

    日本畜産学会大117回大会　2013/09/09

  21. 哺乳仔ウシへの甘草給与による代謝・内分泌の調節機能に関する研究

    佐々木 智子, 小林 諒子, 原田 剛之, 大谷 喜永, 増子 孝則, 平山 琢二, 萩野 顕彦, 盧 尚建, 加藤和雄

    日本畜産学会大117回大会　2013/09/09

  22. Effects of GH SNPs on endocrine status and glucose metabolism in Japanese Black calves

    Kobayashi R, Harjanti DW, Sano H, Hirayama T, Katoh K, Roh SG

    The 64th Annnual Meeting of the European Association for Animal Production　2013/08/26

  23. Effect of propionate, palmitate and insulin on chemerin gene expression in monolayer cultures of bovine hepatocytes

    Sanggun Roh, Shun Kitayama, Suzuki Yutaka, Kyoung-Ha So, Kwon-Jung Yi, Eri Yamauchi, Satoshi Haga, Kazuo Katoh

    2013 ADSA®-ASAS Joint Annual Meeting Abstract Presentation Notice　2013/07/08

  24. Hormonal regulation of α-tocopherol transfer related molecules expression in bovine mammary epithelial cells

    Satoshi Haga, Yosuke Kobayashi, Miwa Nakano, Hiroshi Ishizaki, Sanggun Roh, Kazuo Katoh

    2013 ADSA®-ASAS Joint Annual Meeting Abstract Presentation Notice　2013/07/08

  25. Chemerin expression and secretion in adipocyte and hepatocyte International-presentation

    Suzuki Y, Kitayama S, Yamauchi E, So KH, Katoh K, Roh SG

    NIH-Tohoku University-JSPS Symposium　2013/05/09

  26. Adipogenin null mice shows the retard of the growth and fat accumulation International-presentation

    Hata Y, So KH, Suzuki Y, Katoh K, Roh SG

    NIH-Tohoku University-JSPS Symposium　2013/05/09

  27. The changes of chemerin and chemerin receptor to regulate lipid metabolism in liver and pituitary gland International-presentation

    Roh SG, Kitayama S, Ardiyanti A, Suzuki Y, Yamauchi E, Kato K, Yi KJ, So KH, Hagino A, Katoh K

    EB2013 Meeting　2013/04/20

  28. ウシ乳腺上皮細胞の酸化ストレス応答に及ぼす抗酸化物質の影響

    木下早織, 萩野顕彦, 盧 建尚, 加藤和雄

    日本畜産学会大11６回大会　2013/03/27

  29. 北山 峻、Astrid Ardiyanti山内 恵利、後藤貴文、萩野 顕彦、加藤 和雄、盧 尚建

    ウシ培養肝細胞におけるChemerinの発現と調節因子

    日本畜産学会大11６回大会　2013/03/27

  30. 山内恵利、鈴木 裕、北山 峻、Astrid Ardiyanti、鈴木啓一、加藤和雄、盧尚建

    ケメリン遺伝子内の一塩基多型が黒毛和種牛の枝肉形質および脂肪酸組成に与える影響

    日本畜産学会大11６回大会　2013/03/27

  31. 加藤 大幾、谷田奈々子、Astrid Ardiyanti、鈴木 裕、宋 相憲、加藤 和雄、盧 尚建

    成長ホルモンのCC型黒毛和種牛の培養脂肪細胞の遺伝子発現について

    日本畜産学会大11６回大会　2013/03/27

  32. Importance of single nucleotide polymorphisms (SNPs) in the promoter region of bovine growth hormone gene for beef production in Japanese Black cattle International-presentation

    Katoh, K, Sugita, H, Ardiyanti, A, Suzuki, K, Hirayama, T, Oikawa, T, Shoji, N, Yonekura, S, Roh, S.G

    The 15th AAAP Animal Science Congress　2012/11/25

  33. Three SNPs in the coding region of the bovine chemerin gene and its associations with carcass trait in Japanese Black cattle International-presentation

    Yamauchi, E, Ardiyanti, A, Suzuki, K, Katoh, K, Roh, S.G

    The 15th AAAP Animal Science Congress　2012/11/25

  34. Individual Difference of Serum Oxytocin in Calves and their Dams’ Effect on It International-presentation

    Siyu, C, Tanaka, S, Arino, Y, Chiba, J, Sato, K, Chiba, T, Oyakawa, C, Roh, S.G, Sato, S

    The 15th AAAP Animal Science Congress　2012/11/25

  35. The Effect of Dams on Differences in Serum Oxytocin Levels in Calves International-presentation

    Chen, S, Tanaka, S, Arino, Y, Chiba, J, Sato, K, Chiba, T, Oyakawa, C, Roh, S.G, Sato, S

    The 15th AAAP Animal Science Congress　2012/11/25

  36. 反芻動物の培養肝細胞におけるchemerinの発現調節機構

    北山峻, 菊池佑弥, Astrid Ardiyanti, 山内恵利, 後藤貴文, 萩野顕彦, 加藤和雄, 盧尚建

    東北畜産学会第62回大会　2012/08/30

  37. 黒毛和種牛におけるGH遺伝子多型と脂質代謝関連酵素発現調節

    加藤大幾, 杉田春奈, Astrid Ardiyanti, 鈴木啓一, 盧 尚建, 加藤和雄

    東北畜産学会第62回大会　2012/08/30

  38. Importance of polymorphism in the promoter region of GH gene for beef production and quality International-presentation

    Sugita, H, Ardiyanti, A, Hirayama, T, Suzuki, K, Shoji, N, Yonekura, S, Roh, S.G, Katoh, K

    The 63rd European Association for Animal Production　2012/08/27

  39. アディポジェニン遺伝子欠損マウスにおける脂肪蓄積抑制作用の解析

    蘇 敬夏, 立崎 泰正, 立花 かおる, 畑 優紀, Astrid Ardiyanti, 井上 奈穂, 池田 郁男, 加藤和雄, 盧尚健

    Adiposcience研究会　2012/08/25

  40. The role of oxytocin receptor on adipose tissue lipid metabolism International-presentation

    SG Roh, KH So, Suzuki Y, C Chen, K Katoh

    US ENDO 2012　2012/06/24

  41. 肥満遺伝子の同定とその制御

    日本農芸化学中部支部　2012/05/29

  42. Deletion of adipogenin gene in mice is effected to adipogenesis and deferred to growth International-presentation

    Experimental Biology 2012　2012/04/21

  43. 黒毛和種牛の優良種雄牛クローン子牛の初期成長期における高栄養処理が胸最長筋の遺伝子発現に及ぼす影響

    日本畜産学会大115回大会　2012/03/28

  44. 黒毛和種牛の産肉性に及ぼすGH遺伝子プロモーター領域多型の影響

    日本畜産学会大115回大会　2012/03/28

  45. Apelinはヒツジ下垂体細胞のApelin受容体を介してACTHおよびGH分泌を刺激する

    日本畜産学会大115回大会　2012/03/28

  46. 培養脂肪細胞の脂質蓄積におよぼす5`ーウリジル酸の影響

    日本畜産学会大115回大会　2012/03/28

  47. ウシの脂肪細胞と乳腺上皮細胞におけるオキシトシン受容体（OXT-R）の発現変動

    日本畜産学会大115回大会　2012/03/28

  48. ウシ乳腺上皮細胞およびヒト乳がん細胞の酸化ストレス応答

    日本畜産学会大115回大会　2012/03/28

  49. Intervention of the insulino/somatotropic axes in the development of the digestive function in pre-weaning calves fed sodium (Na)-butyrate supplemented diet International-presentation

    ISNH　2011/09/06

  50. Interaction between GH gene polymorphism and expression of leptin and SCD (stearoyl-coA desaturase) in Japanese Black cattle International-presentation

    Sugimoto, K, Kobashikawa, H, Ardiyanti, A, Sugita, H, Hirayama, T, Suzuki, K, Suda, Y, Yonekura, S, Roh, S.-G, Katoh, K

    EAAP　2011/08/29

  51. 反芻動物における血漿中Apelin濃度の変動と内分泌調節機能の検討

    佐藤勝祥, 大川夏貴, 萩野顕彦, 盧 尚建, 加藤和雄

    日本畜産学会第114回大会　2011/08/26

  52. 反芻動物におけるケメリンの発現制御と糖脂質代謝における生理的作用

    鈴木裕, 加藤和雄, 菊池祐弥, 宋相憲, 佐藤勝祥, 盧 尚建

    日本畜産学会第114回大会　2011/08/26

  53. ヒト肝がん細胞（HepG2）におけるChemerin分泌調節

    盧 尚建, 宋 相憲, 鈴木裕, Asrdiyanti Astrid, 加藤和雄

    BMB2010　2010/12/07

  54. New Regulating Factors in Adipogenesis International-presentation

    Korea-Japan Symposium on Animal Production　2010/09/10

  55. 筋芽細胞および脂肪細胞の脂肪蓄積能におけるAdipogeninの機能解析

    宋 相憲, 鈴木裕, 盧 尚建, 加藤和雄

    Adiposcience研究会　2010/08/21

  56. 脂肪細胞と肝細胞におけるChemerinとChemerin受容体の発現動態

    鈴木裕, 宋 相憲, 盧 尚建, 加藤和雄

    Adiposcience研究会　2010/08/21

  57. Possible functional roles of chemerin, a new adipokine, in domestic animals International-presentation

    Experimental Biology 2010　2010/04/24

  58. The changes of chemerin and chemerin receptor to regulate lipid metabolism in liver and pituitary gland International-presentation

    Sanggun Roh, Shun Kitayama, Astrid Ardiyanti, Yutaka Suzuki, Eri Yamauchi, Daiki Kato, Kwonjung Yi, Kyoungha So, Akihiko Hagino, Kazuo Katoh

    Experimental Biology 2013　2010/04/20

  59. ウシ脂肪細胞におけるケメリン（Chemerin）とその受容体の発現調節機構

    鈴木 裕, 宋 相憲, 盧 尚建, 加藤 和雄

    日本畜産学会第112回大会　2010/03/28

  60. ウシ乳腺上皮細胞におけるアディポネクチンのシグナル伝達とエネルギー代謝及び増殖、生存関連遺伝子発現に及ぼす影響

    大谷 喜永, 宋 相憲, 萩野 顕彦, 盧 尚建, 加藤 和雄

    日本畜産学会第112回大会　2010/03/28

  61. ヒツジとヤギの血中ACTHおよびGH濃度に及ぼす頚静脈内Apelin投与の影響

    佐藤 勝祥, 高橋 辰行, 小岩 幸太, 萩野 顕彦, 盧 尚建, 加藤 和雄

    日本畜産学会第112回大会　2010/03/28

  62. 幼動物へのミルク摂取時に見られる血中GHとインスリン濃度の増加機構

    千田 はるか, 加藤 伸一, 佐藤 勝祥, 高橋 辰行, 星野 由美, 盧 尚建, 加藤 和雄

    日本畜産学会第112回大会　2010/03/28

  63. 黒毛和種肥育牛の枝肉成績・筋肉内脂肪酸組成とFASNおよびSCD遺伝子多型との関連

    杉田 春奈, Ardiyanti Astrid, 横田 祥子, 為岡 奈々恵, 阿部 剛, 庄司 則章

    日本畜産学会第112回大会　2010/03/28

  64. 脂肪細胞由来遺伝子、Adipogeninの発現調節について

    盧 尚建, 宋 相憲, 鈴木 裕, 佐々木 晋一, 加藤 和雄

    日本畜産学会第112回大会　2010/03/28

  65. ウシ乳腺上皮細胞におけるアディポネクチンのシグナル伝達とエネルギー代謝及び増殖、生存関連遺伝子発現に及ぼす影響

    大谷喜永, 宋相憲, 佐藤勝祥, 萩野顕彦, 盧尚建, 加藤和雄

    第32回日本分子生物学会年会　2009/12/09

  66. 哺乳仔ウシの内分泌および消化管機能に及ぼす酪酸給与の影響

    加藤 伸一, 佐藤 勝祥, 大和田 修一, 池田 勝彦, 佐藤 衆介, 盧 尚建, 加藤 和雄

    日本畜産学会第111回大会　2009/09/27

  67. Chemerin, highly expressed in adipose tissues, stimulates te glycerol release in bovine differentiated adipocytes in vitro International-presentation

    Song SH, Fukui K, Hamano K, Sasaki S, Roh SG, Katoh K

    ＸＩｔｈ International Symposium of Ruminant Physiology　2009/09/06

  68. Up-regulation of chemerin and chemerin receptor by TNF-a in bovine differentiated adipocytes in vitro International-presentation

    Roh SG, Song SH, Katoh K

    36th International congress of physiological sciences　2009/07/27

  69. Possible functional roles of chemerin, a new adipokine, in International-presentation

    Song SH, Suzuki Y, Roh SG, Katoh k

    EB2009　2009/04/18

  70. ランドレース種と梅山豚種の日か脂肪組織における遺伝子発現プロファイリングの比較

    中村 允哉, 佐藤 智美, 福井 一晃, 中島 郁世, 千国 幸一, 佐々木 晋一, 盧 尚建

    日本畜産学会第110回大会　2009/03/27

  71. Cloning and expression of bovine chemerin and chemerin receptor International-presentation

    Roh SG, Song SH, Fuki K, Satoh T, Katoh K, Sasaki S

    International Conference of Farm Animal Endocrinology　2008/11/13

  72. C2C12筋芽細胞の脂肪蓄積能におけるAdipogenesisの役割

    宋 相憲, 佐藤 智美, 佐々木 晋一, 盧 尚建

    日本畜産学会第109回大会　2008/03/27

  73. めん羊脂肪細胞分化の自己調節機構に及ぼすVFAの役割

    目黒 壮, 北村 健, 山本 俊輔, 鈴木 玲央奈, 盧 尚建, 佐々木 晋一

    日本畜産学会第109回大会　2008/03/27

  74. めん羊成熟脂肪細胞におけるTNF-α分泌に及ぼす各種脂肪酸の影響 - TNF-αの脂肪細胞増殖・分化制御機構 -

    佐々木 晋一, 杉山 聡子, 目黒 壮, 盧 尚建

    日本畜産学会第109回大会　2008/03/27

  75. マウス精子形成におけるhemidesmosome junction構成員氏：dystoninの関与

    高橋 大, 河西 多恵, 甲木 潤, 盧 尚建, 小野 珠乙, 佐々木晋一, 辻井 弘忠, 濱野 光市

    第100回日本繁殖生物学会大会　2007/10/18

  76. マウス精子の走温性におけるTransient Receptor Potential Vanilloid (TRPV) 4の関与の関与

    河西 多恵, 鈴木 誠, 水野淳子, 高橋 大, 甲木 潤, 橋本 聡, Quzi Sharmin Akter, 盧 尚建, 佐々木晋一, 辻井 弘忠, 濱野 光市

    第100回日本繁殖生物学会大会　2007/10/18

  77. ウシ脂肪細胞におけるGPR40およびGPR120遺伝子の発現動態

    中村 允哉, 佐藤 智美, 目黒 壮, 鈴木 康幾, 宋 相憲, 後藤 千寿, 伊賀 朋世, 盧 尚建, 佐々木 晋一

    日本畜産学会第107回大会　2007/03/27

  78. 牛の脂肪細胞分化・形成、その過程での重要な遺伝子発現について

    盧 尚建, 後藤 千寿, 伊賀 朋世, 佐々木晋一

    平成18年度問題別研究会　2006/11/10

  79. ヤギ清上皮におけるFSHレセプターmＲＮＡの発現

    高橋 大, 船井 咲知, 河西 多恵, 後藤 千寿, 盧 尚建, 小野 珠乙, 佐々木晋一, 辻井 弘忠, 名倉 義夫, 藤田 優, 濱野 光市

    北信越畜産学会 第55回大会　2006/10/26

  80. マウス精子の走温性におけるTransient Receptor Potential Vanilloid (TRPV) 4の投与

    河西 多恵, 高橋 大, 船井 咲知, 鈴木 誠, 水野 淳子, 後藤 千寿, 盧 尚建, 佐々木晋一, 辻井 弘忠, 濱野 光市

    北信越畜産学会 第55回大会　2006/10/26

  81. The Possible Physiological Roles of GPCR120 on Adipogenesis

    Gotoh C, Suzuki Y, Song SH, Nakamura M, Iga T, Roh SG, Sasaki S

    The 11th Adiposcience Symposium　2006/08/19

  82. ゲノムデータベースの高度化利用による脂肪細胞分化形成に関わる機能未知遺伝子群の分離と同定

    宮原 久恵, 西村 幸彦, 後藤 千寿, 伊賀 朋世, 鈴木康幾, 宋 相憲, 盧 尚建, 佐々木 晋一

    家畜栄養生理研究会春季大会　2006/03/30

  83. 長鎖脂肪酸をリガンドとするＧタンパク質共役受容体GPCR40およびGPCR120の発現動態

    後藤 千寿, 宮原 久恵, 鈴木 康幾, 伊賀 朋世, 宋 相憲, 盧 尚建, 佐々木晋一

    日本畜産学会第106回大会　2006/03/29

  84. ウリジル酸の脂肪細胞分化過程に及ぼす影響

    伊賀朋世, 西村幸彦, 増子孝則, 加藤和雄, 小原嘉昭, 盧 尚建, 佐々木晋一

    日本畜産学会第106回大会　2006/03/29

  85. ウシadipogenin遺伝子のクローニングと脂肪細胞分化・形成過程における発現パターン

    盧 尚建, 萩原 由美, 宮原 久恵, 後藤 千寿, 伊賀 朋世, 鈴木 康幾, 宋 相憲, 佐々木晋一

    日本畜産学会第106回大会　2006/03/29

  86. めん羊脂肪細胞の増殖・分化制御における転写因子、PPARの生理学的意義

    杉山 聡子, 富松 愛, 北村 健, 伊賀 朋世, 洪 娟喜, 盧 尚建, 佐々木 晋一

    日本畜産学会第105回大会　2005/09/09

  87. CLA、trans-10,cis-12 isomerのめん羊前駆脂肪細胞におけるadipogenesis促進作用機構

    伊賀 朋世, 杉山 聡子, 北村 健, 洪 娟喜, 後藤 千寿, 盧 尚建, 佐々木 晋一

    日本畜産学会第105回大会　2005/09/09

  88. Plasma-type Gelsolinによる脂肪細胞分化・形成の制御機構

    都築啓晃, 洪娟喜, 菱川大介, 宮原久恵, 西村幸彦, 荻原由美, 鈴木康幾, 野中淑恵, 盧尚建, 佐々木晋一

    日本畜産学会第104回大会　2005/03/27

  89. 遺伝子データベースを利用した脂肪組織由来因子探索の有用性の検討

    菱川 大介, 洪 娟喜, 都築 啓晃, 宮原 久恵, 西村 幸彦, 後藤 千寿, 盧 尚建, 佐々木 晋一

    日本畜産学会第104回大会　2005/03/27

  90. 脂肪酸をリガンドとするＧタンパク共役受容体を介する脂肪細胞分化・形成過程の機能解析

    後藤 千寿, 菱川 大介, 洪 娟喜, 都築 啓晃, 西村 幸彦, 宮原 久恵, 鈴木 康機, 野中 淑恵, 萩原 由美, 伊賀朋世, 盧 尚建, 佐々木晋一

    日本畜産学会第104回大会　2005/03/27

  91. 脂肪細胞特異的膜タンパク質adipogeninの脂肪細胞分化調節機構の解析

    洪 娟喜, 菱川 大介, 宮原 久恵, 都築 啓晃, 西村 幸彦, 後藤 千寿, 帆刈 優, 高木 優二, 盧 尚建, 佐々木晋一

    日本畜産学会第104回大会　2005/03/27

  92. Ｇタンパク質共役受容体 GPR43を介する脂肪細胞分化・形成の分子制御機構

    西村幸彦, 盧 尚建, 菱川大介, 都築啓晃, 宮原久恵, 洪 娟喜, 後藤千寿, 杉山聡子, 北村 健, 富松 愛, 佐々木 晋一

    第27回日本分子生物学会年会　2004/12/08

  93. The novel gene U2 regulates adipocyte differentiation and fat deposition

    Hong YH, Roh SG, Gotoh C, Hishikawa D, Miyahra H, Nishimura Y, Tsuzuki H, Sasaki S

    第27回日本分子生物学会年会　2004/12/08

  94. 脂肪細胞特異的膜タンパク質ＳＭＡＦ１の脂肪細胞分化調節機構の解析

    宮原 久恵, 盧 尚建, 菱川 大介, 都築 啓晃, 洪 娟喜, 西村 幸彦, 後藤 千寿, 帆刈 優, 高木 優二, 佐々木 晋一

    第9回アディポサイエンス研究会シンポジウム　2004/08/21

  95. 脂肪蓄積および脂肪細胞分化におけるTIG2とGPCR-DEZの生理学的意義

    菱川 大介, 盧 尚建, 宮原 久恵, 洪 娟喜, 都築 啓晃, 西村 幸彦, 後藤 千寿, 佐々木 晋一

    第9回アディポサイエンス研究会シンポジウム　2004/08/21

  96. 脂肪細胞分化・形成におけるGPR43の生理学的意義

    西村幸彦, 盧 尚建, 菱川大介, 宮原久恵, 洪 娟喜, 都築啓晃, 後藤千寿, 杉山聡子, 富松 愛, 佐々木 晋一

    第1回GPCR研究会　2004/05/08

  97. 脂肪細胞形成および脂肪沈着の局在化に関わる機能未知遺伝子群の同定とその機能解析

    宮原 久恵, 盧 尚建, 菱川 大介, 洪 娟喜, 都築 啓晃, 富松 愛, 久野 正明, 佐々木 晋一

    日本畜産学会第103回大会　2004/03/29

  98. 三次元コラーゲンゲルマトリックス培養によるめん洋成熟脂肪細胞の培養能

    佐々木 晋一, 杉山 聡子, 北村 健, 富松 愛, 洪 娟喜, 盧 尚建

    日本畜産学会第103回大会　2004/03/29

  99. 培養めん羊皮下脂肪細胞におけるレプチン分泌動態

    杉山 聡子, 北村 健, 富松 愛, 洪 娟喜, 盧 尚建, 佐々木 晋一

    日本畜産学会第103回大会　2004/03/29

  100. 脂肪細胞形成に伴うGelsolinの発現とその意義

    都築 啓晃, 盧 尚建, 菱川 大介, 久野 正明, 富松 愛, 宮原 久恵, 西村 幸彦, 洪 娟喜, 佐々木 晋一

    日本畜産学会第102回大会　2003/09

  101. ウシとブタの脂肪蓄積部位間における発現遺伝子プロファイリング

    盧 尚建, 久野 正明, 菱川 大介, ヨゲンドラ シュレスタ, 崔 基春, 佐々木 晋一

    日本畜産学会第100回大会　2003/09

  102. 脂肪細胞形成におけるグレリンとグレリン受容体の機能解析

    盧 尚建, 菱川 大介, 久野 正明, 西村 幸彦, 崔 基春, 佐々木 晋一

    2003年度 日本農芸化学会　2003/03/29

  103. 脂肪蓄積過程における非筋型コフィリンの生理学的役割

    菱川 大介, 盧 尚建, 宮原 久恵, 洪 娟喜, 久野 正明, 都築 啓晃, 崔 基春, 佐々木 晋一

    2003年度 日本農芸化学会　2003/03/29

  104. 脂肪細胞形成に関する新規遺伝子の機能解析

    盧 尚建, 洪 娟喜, 菱川 大介, 久野 正明, 都築 啓晃, 富松 愛, 崔 基春, 左 久, 佐々木 晋一

    日本畜産学会第101回大会　2003/03/27

  105. ルーメン発達に関与する候補遺伝子群の解析

    久野 正明, 盧 尚建, 西村 幸彦, 菱川 大介, 都築 啓晃, 富松 愛, 洪 娟喜, 加藤 和雄, 小原 嘉昭, 佐々木 晋一

    日本畜産学会第101回大会　2003/03/27

  106. 遺伝子発現プロファイルを用いた脂肪蓄積機構の解析

    菱川 大介, 盧 尚建, 宮原 久恵, 洪 娟喜, 久野 正明, 都築 啓晃, 富松 愛, 崔 基春, 佐々木 晋一

    日本畜産学会第101回大会　2003/03/27

  107. めん羊脂肪細胞におけるインスリン誘引脂質分解作用に関与するインスリンの細胞内情報伝達機構

    佐々木 晋一, 盧 尚建

    日本畜産学会第101回大会　2003/03/27

  108. ウシとブタの脂肪蓄積部位間における発現遺伝子プロファイリング

    盧 尚建, 久野 正明, 菱川 大介, ヨゲンドラ シュレスタ, 崔 基春, 佐々木 晋一

    日本畜産学会第100回大会　2002/03/28

  109. めん羊前駆脂肪細胞の分化に伴うグレリン受容体の変動

    ヨゲンドラ シュレスタ, 盧 尚建, 久野 正明, 菱川 大介, 崔 基春, 佐々木 晋一

    日本畜産学会第100回大会　2002/03/28

  110. Differential Display法による反芻動物のルーメン由来遺伝子の探索

    久野 正明, 盧 尚建, 菱川 大介, ヨゲンドラ シュレスタ, 崔 基春, 佐々木 晋一

    日本畜産学会第100回大会　2002/03/28

  111. 黒毛和種牛における脂肪由来遺伝子のクローニング

    菱川 大介, 盧 尚建, ヨゲンドラ シュレスタ, 崔 基春, 久野 正明, 佐々木 晋一

    日本畜産学会第100回大会　2002/03/28

  112. 豚における皮下および腹腔内脂肪組織間の発現遺伝子の比較検討

    崔 基春, 盧 尚建, 久野 正明, ヨゲンドラ シュレスタ, 菱川 大介, 佐々木 晋一

    日本畜産学会第100回大会　2002/03/28

Show all Show first 5

Research Projects 29

  1. 黒毛和種仔牛の生体情報の利活用事業

    Offer Organization: 日本中央競馬会畜産振興

    2023/04 - 2026/03

  2. 精子の質的不均一性発生機構の解明

    原 健士朗, 盧 尚建

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2021/07/09 - 2023/03/31

    More details Close

    2021年は、以下の解析を実施し、雄体内における精子の品質制御メカニズムの解析技術の開発を進めた。まず、精子の前駆細胞の膜上に時期特異的に蛍光標識を入れるためのCreERマウスの作製を進めた。既報のRNAシークエンス解析のデータセットから分化細胞で特異的に発現する遺伝子を１つ選択し、その発現を免疫組織化学染色で確認した後、同遺伝子のプロモータの下流にCreERを導入したベクターを作製後、培養下の胚性幹（ES）細胞内での相同組換えを行った。得られた組換えES細胞を正常型の胚盤胞にインジェクションし、現在までに毛への高い寄与が確認されたキメラを2系統作成し、F1を得るための交配試験を実施している。キメラマウスについて、生殖系列へも高い寄与が予想されるが、系統数が少なく、うまくF1が得られない場合に備え、追加のキメラ作製も検討中である。次に、Creによる組換えによって特異的に精子の前駆細胞を蛍光標識できるレポータマウスを検討した。具体的には、生殖細胞の細胞膜に蛍光タンパク質を取り込める既報のレポーターマウスを第一候補として導入した。上述のCreERマウスおよびレポーターマウスを掛け合わせることで、2022年に精子品質制御機構解析のモデル動物を開発する。なお、今後の解析に必要なイメージング技術の改良および内分泌影響の予備検討も実施した。以上の研究は、雄体内における精子の品質のばらつきの発生要因解析に資する技術基盤であり、この利活用を通じて野生動物や産業動物の雄繁殖能制御に資する成果が得られることが期待される。

  3. 日本短角種の高品質赤肉生産開発事業

    Offer Organization: 日本中央競馬会畜産振興

    2019/04 - 2022/03

  4. 子ウシの健全な発達を目指した消化管の上皮細胞接着微生物叢の相互関係の解明

    Offer Organization: 伊藤記念財団

    2020/04 - 2021/03

  5. メタン産生量の個体差に影響する諸要因の解析（令和2年度畜産分野における気候変動緩和技術の開発委託事業）

    Offer Organization: 農林水産省

    2018/04 - 2021/03

  6. Development of new rumen immunology by elucidating the interrelationship between rumen tissue and host immune system

    ROH SANGGUN

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2018/04 - 2021/03

    More details Close

    This study was to clarify the interaction between the immune response, homeostasis, and the host immune system that occur in rumen epithelium. The results are as follows:(1) CCL5 elevated after weaning is thought to induce monocyte migration and differentiation into macrophages, and the CCL5-monocyte / macrophage axis may play an important role in maintaining homeostasis in rumen epithelium. (2) In the rumen epithelium, IL-1β contributes to the maintenance of homeostasis when SCFA, LPS, flagellin, etc. increase in the rumen due to weaning and the environment by the environment changes in rumen, and in addition it is suggested to contribute to the repair of damage by promoting cell proliferation.

  7. 「革新的技術開発、緊急展開事業（うち先導プロジェクト）」、国産和牛肉の新たな差別のための評価指標及び育種手法の開発

    Offer Organization: 国立研究開発法人 農業・食品産業技術総合研究機構生物系特定産業技術研究支援センター

    2016/04 - 2021/03

  8. 「革新的技術開発、緊急展開事業（うち先導プロジェクト）」、和牛肥育期間の大幅な短縮技術の開発

    Offer Organization: 国立研究開発法人 農業・食品産業技術総合研究機構生物系特定産業技術研究支援センター

    2016/04 - 2021/03

  9. The analysis of molecular mechanism and the application on the differentiation, proliferation and growth of rumen papillae

    Roh Sanggun

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2015/04/01 - 2017/03/31

    More details Close

    This study was aimed to identify the genes associated with the development of the rumen epithelium between suckling and weaned Japanse Black cattle. Digital differential display analysis in silico can be useful for screening candidate genes related to the rumen development, and 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), aldo-keto reductase family 1, member C1-like (AKR1C1), and fatty acid binding protein 3 (FABP3) showed significant changes in the levels of gene expression in the rumen between suckling and weaned calves. In addition, RNA seq analysis that 871 genes in rumen papillae of suckling and weaned calves by RNA seq analysis were changed. IPA analysis showed that the production of ketone bodies were increased and PPAR was activated after weaning.

  10. 黒毛和種牛のルーメン絨毛組織の発達関連遺伝子の網羅的解析に関する研究

    Offer Organization: 伊藤記念財団

    2015/04 - 2016/03

  11. The analysis of interaction of chemerin and other adipokines in bovine fat accumulation and lipid metabolim

    ROH SANGGUN, SUZUKI Kei-ichi, Nakajima Kei-ichi, HAGINO Akihiko, HAGA Satoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2013/04 - 2016/03

    More details Close

    This study was aimed to elucidate the physiological function of chemerin in the metabolism transitional period of weaning and lactating dairy cattle. Protein expression of Chemerin in the liver was reduced after weaning, but chemerin regulated the gluconeogenesis by propionic acid in the liver without the regulation of insulin secretion in the weaning period. Chemerin gene expression and secretion was reduced in liver of dairy cows after delivery, but this change is directly related with the regulation hepatic gluconeogenesis. This results suggest that the expression and secretion of chemerin in cattle dynamically changes the metabolism transition and revealed to be involved in the regulation of lipid metabolism or glucose metabolism.

  12. Fattening and evaluation of Japan Japanese Black Cattle selected on the basis of the fat metabolism-related gene mutation

    KATOH KAZUO, HIRAYAMA Takujie, ROH Sanggun

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2013/04 - 2016/03

    More details Close

    This study was to conduct the fattening evaluation by characterizing the fat accumulation and endocrine regulation in single nucleotide polymorphism of bovine growth hormone (bGH). (1) GH gene polymorphism changed the oleic acid composition in the intramuscular fat by regulating the SCD-1. (2) Response to GH stimulation was different depending on the GH genotype. (3)Insulin has an action to suppress the secretion of GH acts directly on the anterior pituitary, GH secretion is increased by changes in stress and NEFA concentrations caused by the sharp reduction in blood glucose levels in vivo. These results indicate that endocrine regulations such as insulin may different by GH polymorphism of Japanese Black Cattle, affecting the meat quality and production due to changes in fat accumulation.

  13. ウシの代謝・内分泌ネットワークにおけるケメリンの分泌調節作用の解明

    Offer Organization: 財団法人旗影会

    2014/04 - 2015/03

  14. Swine derived induced pluripotent stem cell and its application to livestock science

    FUKUDA Tomokazu, UENISHI Hirohide, HOSHINO Yumi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2011/04 - 2015/03

    More details Close

    In this study, we applied the induced pluripotent stem (iPS) cell technology to the livestock science. We focused on the porcine, one of the major livestock, and established the porcine derived iPS cell. We obtained porcine derived iPS cells with the expression of six reprogramming factors, including Oct3/4, Klf4, Sox2, c-Myc. The porcine derived iPS cell had different nature from that of human, and it showed the growth dependency to the LIF (leucocyte migration inhibition factor), and low molecular inhibitors. Interestingly, porcine derived iPS did not cause the massive apoptosis even after the enzymatic digestion into the single cell, which nature is different from human. Furthermore, the re-activation of X chromosome was observed in female derived porcine iPS cells. We succeeded the high quality porcine derived iPS cell, which is close to the nature of early embryonic stage.

  15. Offer Organization: JST

    System: 究成果最適展開支援プログラムA-STEP フィージビリティスタディステージ 探索タイプ

    2013/04 - 2014/03

  16. 次世代型の革新的家畜生産システムを構築する若手研究者の戦略的育成

    Offer Organization: 日本学術振興会

    System: 頭脳循環を加速する若手研究者戦略的海外派遣プログラム,

    2012/04 - 2014/03

  17. Analysis of new endocrine regulatory system between posterior pituitary gland and adipose-mammary gland tissues via oxytocin receptor

    ROH Sanggun, NISHIMORI Katsuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Category: Grant-in-Aid for Challenging Exploratory Research

    Institution: Tohoku University

    2011 - 2012

    More details Close

    The aim of this study was to investigate 1) the expression of OXTR in adipose tissues and possible role in regulating lipid metabolism, and 2) the expression of OXTR during the differentiation of mammary epithelial cells. These results suggest 1) that up-regulation of OXTR during fat accumulation and adipocyte development may intensify the catabolic effects by oxytocin in adipose tissue to cause lipolysis, 2) OXTR expressionwas regulated by adiponectin and lactogenic hormones in bovine mammary epithelial cells.

  18. Endocrine and metabolic studies on single nucleotide polymorphism of GH gene in Japanese Black cattle

    KATOH Kazuo, SANO Hiroaki, ROH Sang-gun, HIRAYAMA Takuji, HAGINO Akihiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2010 - 2012

    More details Close

    1) We successfully established an applied method of Dot-plot-SNP for analyzing GH single nucleotide polymorphisms (SNPs), 2) In the studies on gene expression of fatty acid synthesis enzymes (FASN, SCD and SREBP-1), adipocytes isolated from the animal with type CC demonstrated greater sensitivity to GH stimulation than those with another type, 3) Glucose-clamp analysis demonstrated greater insulin sensitivity in the animals with the type AA than that with type BB.

  19. 動物ゲノム情報を活用した新需要創造のための研究のうち「ブタの皮下脂肪厚を制御する遺伝子の解明」,

    Offer Organization: 独立行政法人農業・食品産業技術総合研究機構・畜産草地研究所

    2007/04 - 2011/03

  20. Characterization of molecular mechanisms of bovine fat accumulation by global gene expression analysis

    ROH Sanggun, GOTOH Takfumi, CHIKUNI Koichi, HIRAMATSU Kohzi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Shinshu University

    2008 - 2010

    More details Close

    This study was to investigate the molecular mechanism of fat accumulation and adipogenesis by DNA chip using adipose tissues from 10- and 24-month-old Japanese Black Cattle. Several candidate genes were separated and identified from global gene expression analysis. Adipogenin, chemerin and mPARM-1 genes were confirmed to relate to lipid metabolism and adipocyte differentiation in bovine adipocytes. These results suggested that several genes were involved with adipogenesis and fat accumulation in bovine.

  21. STUDY ON SNP OF GH GENES FAVORING BEEF PRODUCTION

    KATO Kazuo, ROH Sang-gun, SUZUKI Keiichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2007 - 2009

    More details Close

    We investigated the relationship between GH gene SNP and beef production in Japanese Black cattle. (1) C-type allele was for fat production in high-fat meat although A-type allele was profitable for milk production. (2) A-type allele was profitable for carcass weight, dairy gain, BMS and so on, while C-type allele was profitable for unsaturated fatty acid production in meat. (3) A-type allele showed a significant relationship with greater gene expression of SCD and FASN, while C-type allele showed that with lower SCD expression. (4) GHRH-induced GH release in C-type animals was smallest among all types. (5) The effects on meet production was greater for heifers than for bulls.

  22. 逆生理学的研究手法を用いた反芻動物の脂質代謝調節因子の同定と機能解析

    盧 尚建

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 萌芽研究

    Category: 萌芽研究

    Institution: 信州大学

    2007 - 2008

    More details Close

    我々は脂肪組織で発現する新規アデイポカイン(Adipokine)としてマウスケメリン(Chemerin)とその受容体を報告している。平成20年度は、ウシケメリンとその受容体の遺伝子をクローニングし、塩基配列の解析を行った。GenomeNetを用いたBlastan解析を行ったところ、ウシケメリンとケメリン受容体と思われる遺伝子配列が得られた。これらの配列を基に、特異的プライマーを作成し、ウシケメリンとケメリン受容体全長を決定した。黒毛和種牛の組織からtotal RNAを抽出し、ウシケメリン遺伝子分離と遺伝子発現に供した。ウシケメリンcDNAは3個のExonからなり、ORFは489bpであった。得られた配列はヒトとマウスの塩基配列との相同性は、各々79%と69%であり、アミノ酸配列の相同性は、各々74%と60%であった。ケメリン受容体のORFは1089bpであり、ヒトとマウスの塩基配列と84%と79%の相同性を示した。各組織ごとにおけるケメリンの遺伝子発現量については、肝臓と脂肪組織で高い発現が見られたが、ケメリン受容体は脂肪組織で高い発現量を示したものの、各組織において差は見られなかった。ウシ分化脂肪細胞において、ケメリンとケメリン受容体の遺伝子発現量は脂肪細胞分化に伴い発現量は上昇した。また、分化脂肪細胞でのTNF-αの処理はケメリンとケメリン受容体の遺伝子発現量を増大させた。以上のことから、ウシ脂肪組織でも同様に脂肪細胞由来のアデイポカインが存在し、脂質代謝系に影響を与える可能性が示唆された。

  23. Explorations and Functional Analysis of gene related with fat accumulation

    ROH Sanggun, SASAKI Shin-ichi, ONO Tamao

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Shinshu University

    2005 - 2007

    More details Close

    In order to build the system of good beef production of Japanese Black Cattle, understanding of fat accumulation among each adipose tissue is important. Therefore, the candidate genes related with fat depots have investigated in Japanese Black Cattle. (1) The gene of CDC42, prefoldin-5, decorin, phosphate carrier, 12S ribosomal RNA gene, and Kbtbd2 was identified by the analysis of the gene expression profile between subcutaneous and visceral adipose tissue using the differential display RT-PCR method. Expression patterns of these genes in Japanese Black Cattle were different with those of a single stomach animal like a pig or a mouse. (2) GPCR43, Adipogenin and GPCR120 were highly expressed in adipose tissue and up-regulated during adipocyte differentiation. GPCR43, Adipogenin, GPCR120, and Claudin-6 were important genes on adipocyte differentiation and adipose tissue development (4) Bovine Adipogenin isoform cDNA received alternatives splicing as a result of base sequence analysis, and it was confirmed that the base sequence is missing as compared with Adipogenin. Bovine isoform cDNA was highly expressed in adipose tissue and up-regulated during the adipocyte differentiation of bovine. This was similar with the results of the Adipogenin gene. A possibility that the isoform of an Adipogenin gene was involved with adipose tissue developments was suggested. (5) Introducing an Adipogenin gene into a C2C12 cell showed the fat accumulation in cells without the changes of genes related with adipogenesis.

  24. トランスクリプトーム解析による脂質蓄積関連遺伝子群の探索と機能解析

    Offer Organization: ホクト生物科学振興財団

    2005/04 - 2006/03

  25. Transfer of genome information to reconstitute endangered birds

    ONO Tamao, KAGAMI Hiroshi, ROH Sanggun

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Shinshu University

    2004 - 2006

    More details Close

    The aim of this project was the production and reserve of endangered, rare and valuable birds using the technique of germ cell transfer. We developed the protocol to obtain a large amount of PGCs from gonads of chicken and quail embryos using PGC specific monoclonal antibodies (anti-SSEA1, 2C9 and QCR1) and magnetism activated cell sorting (MACS). MACS treatment greatly increased the population ratio of avian PGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. Blastodermal and primordial germ cells were transferred to the recipient avian embryos. Treated chimeras were hatched and the donor-derived cells were tracked using species-specific DNA markers. Donor derived genome was observed using PCR and Immunohistochemistry in chimeric quail which introduced blastodermal cells of chicken and blue-breasted quail embryos.

  26. 前駆脂肪細胞の増殖・分化におけるグレリンの生理学的役割

    Offer Organization: 伊藤記念財団

    2003/04 - 2005/03

  27. アディポジェネシスにおけるグレリンとグレリン受容体の生理学的役割

    盧 尚建

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 若手研究(B)

    Category: 若手研究(B)

    Institution: 信州大学

    2003 - 2004

    More details Close

    成長ホルモンの分泌は、これまで視床下部ホルモンであるGHRH(促進)とソマトスタチン(抑制)によって制御されていると考えられていた。一方、GHS受容体(GHS-R)と呼ばれるオーファン受容体を介した制御機構も存在することが報告され、新たな内因性成長ホルモン分泌刺激ホルモンとして胃分泌物質、グレリンが発見された。しかし、グレリンの脂肪細胞分化・形成(アディポジェネシス)、脂肪細胞における作用機構およびグレリン受容体の発現機序についてはまだ不明である。したがって、我々は、脂肪細胞におけるグレリンとGHS-Rの関係を明らかにするために、めん羊およびラット脂肪細胞の生成過程に対する成長ホルモン刺激受容体アンタゴニストである[D-Lys-3]-GHRP-6の作用を調査した。[D-Lys-3]-GHRP-6はめん羊およびラット脂肪組織由来の前駆脂肪細胞の脂肪細胞分化を有意に抑制した。また、脂肪細胞分化のマーカーであるPPAR-γ2の発現量は[D-Lys-3]-GHRP-6を10日間添加することによって減少した。逆に、グレリンは脂肪細胞分化過程においてGPDH酵素活性を増加させ、PPAR-γ2の発現量を上向き調節することにより、脂肪細胞分化を促進した。また、めん羊およびラット脂肪組織から単離した脂肪細胞において、グレリンによる脂質分化抑制能は[D-Lys-3]-GHRP-6添加によって減弱された。我々の結果はGHS-Rを介する脂肪細胞分化・形成過程が、[D-Lys-3]-GHRP-6によって抑制されることを明らかにしたものである。

  28. 成長ホルモン放出ペプチドによる脳下垂体の成長ホルモン分泌機構の分子生物学的解析

    Offer Organization: 日本科学財団

    2002/04 - 2003/03

  29. レプチンによる脳下垂体の成長ホルモン分泌制御の分子機構

    蘆 尚建

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 若手研究(B)

    Category: 若手研究(B)

    Institution: 信州大学

    2001 - 2002

    More details Close

    本研究は脳下垂体におけるレプチンの成長ホルモン分泌抑制に対する分子生物学的解析を行うことを目的としている。脳下垂体のGH細胞を分離、初代培養を施行し、以下の実験に供する。培養細胞に一定の時間と間隔でレプチンの投与後、total RNAを抽出、GH放出因子とグレリン受容体(成長ホルモン刺激物質受容体)mRNAレベルを測定した。レプチンをヒツジの脳下垂体に短期間(1時間)投与した時には成長ホルモンとGHRHによる成長ホルモン分泌に影響がなかった。長期間(24時間)投与すると10^<-7>-M GHRHによる成長ホルモン分泌を抑制した。レプチンを24、48、72時間投与すると、GHやGH放出因子受容体のmRNAレベルが用量依存的に減少した。しかし、グレリン受容体は24と48時間投与した時には変化がなかったが、72時間投与すると有意に増加した。さらに、レプチンを3日間投与するとGHRHによるGH分泌能は減少したが、GHRP-2によるGH分泌能はやや増加した。しかし、GHRHとGHRP-2を同時に投与した時にはレプチンによるGH分泌能には変化がなかった。以上の結果は2001年Endocrinology 142巻の5167ページに掲載された。 さらに、GH放出因子受容体とグレリン受容体の相互作用をAntisense oligonucleotideを用いて分子レベルで検討し、GH放出因子受容体はGHRP-2によって制御される結果を得て、2002年度Endocrinology143巻の1964ページに発表した。また、この研究費の一部分は、脂肪細胞形成においてグレリン受容体の機能解析に用いられ、グレリン受容体は脂肪細胞分化と伴い増加する結果を得て2002年Animal Science Journal(73巻の305ページ掲載)に発表した。

Show all Show first 5

Social Activities 1

  1. 南相馬市の被爆牛のCs蓄積量の調査

    2011/07/01 - Present

    More details Close

    南相馬市の被爆牛を解体し、各組織のCs蓄積量を調査する。

Other 15

  1. 家畜の産肉性と生産性を特徴づける脂肪細胞由来の新規調節因子の解明に関する研究

    More details Close

    食肉の肉質と美味しさは多数の要因によって決定されるが、「脂肪交雑」と「脂肪の質」などによって主に評価される。したがって、家畜の産肉性と生産性の向上には、体内での筋肉内脂肪、皮下脂肪、内臓脂肪組織における脂肪細胞の形成と蓄積過程に関わる新規調節因子の解明は重要である。本研究ではこれまで脂肪細胞由来の脂肪蓄積に関連する因子について同定、解析および生産性との関連を調査した。

  2. 国際競争力強化に向けた黒毛和種短期肥育技術の開発

    More details Close

    肥育開始月齢を８か月齢280kgとし、早期肥育に向く子牛育成方法を開発する。その後の肥育期間を18か月とし26か月齢で出荷できるようにCPやTDNを調整した管理を行い、この間の栄養状態を把握する。対照として慣行肥育を行い、生産された牛肉の解析を行い、差異について検討する。第2回目以降の肥育においては、1回目のデータを検討しプログラムの修正を行うとともに、明らかとなった基礎的知見をもとにビタミンなどの補給も試みる。

  3. 国産和牛肉の新たな差別化のための評価指標及び育種手法の開発

    More details Close

    和牛肉の構成成分等を詳細かつ網羅的に分析することにより、和牛肉に特徴的な成分項目を抽出し、新たな牛肉評価指標を作成する。和牛のゲノム情報を網羅的に解析し、選抜に有効なＳＮＰマーカーを開発するとともに、開発したＳＮＰマーカー周辺を探索し、遺伝子機能を明らかにした上で和牛肉の特性に関連する遺伝子を解明する。新たな指標に基づく和牛の育種改良技術を開発するとともに、市場における和牛肉の優位性を確保する。

  4. 黒毛和種牛のルーメン絨毛組織の発達関連遺伝子の網羅的解析に関する研究

    More details Close

    反芻動物のルーメンは、ヒトやマウスなどの単胃動物と異なり、飼料の発酵に重要な役割を果たしている器官である。特に、仔牛の離乳前後における適切な栄養・飼養管理によるルーメン発達を促すことは重要であり、丈夫なルーメンづくりとは、その容積とともに筋肉層と上皮層（第一胃乳頭・絨毛）の両方を発育させることである。本研究は次世代シーケンサーを用いてウシのルーメン絨毛組織の形成・発達に関連する候補遺伝子を選別し、多様な飼養条件（エピジェネティクス制御）で得られたウシのルーメン絨毛組織において遺伝子の機能と解析を行うものである。

  5. Animal Science and Technology 編集委員

  6. National Institute of Animal Science, RDA Honary researcher

  7. ウシの代謝・内分泌ネットワークにおけるケメリンの分泌調節作用の解明

    More details Close

    本研究は、ウシの代謝・内分泌調節機構におけるケメリンの新たな生理学的な役割を解明するために、①肥育牛と乳牛において脂肪蓄積と糖脂質代謝に対するケメリンの分泌と他のアディポカインの相互作用（Adipokinome：アディポカインの網羅的解析）を調査し、②培養脂肪細胞、肝細胞と下垂体細胞におけるケメリンの作用と自己分泌調節作用を明らかにすることが目的である。

  8. 牛肉中のオレイン酸蓄積を増大するGH遺伝子多型の簡易検出法の開発

    More details Close

    脂質を豊富に含む牛肉生産に適した黒毛和種牛は、ヨーロッパ種牛には存在しないC型の成長ホルモン（GH）遺伝子多型を特異的に有している。このC型遺伝子が不飽和脂肪酸（オレイン酸など）に富む牛肉生産を促進することが報告されており、この遺伝子の安価で簡易な検出・利用は経済的および健康的波及効果に資すると考えられる。本研究では、高価で時間の要する従来の遺伝子解読技術の代わりに、C型遺伝子の有無を安価で迅速に判断できる現場対応型の新規技術開発を目的とする。

  9. 被爆家畜の放射線セシウム蓄積量のモニタリング

    More details Close

    本研究は１）20km警戒区域内で被爆された牛から血液、尿、、胃内物、内臓や筋肉などを採取し、各組織へのセシウムを含む放射線セシウムの蓄積を調査することと、２）20km警戒区域外で放射線に曝露されたヤギへの非汚染飼料の摂取による放射線蓄積量の変動を観察することを目的とする。

  10. ウシの脂肪交雑に関わる因子の探索

    More details Close

    黒毛和種牛と韓牛の脂肪交雑に関わる因子の探索

  11. ブタの免疫関連の抗生物質の代替物の有効性の生体外における検証方法の開発

    More details Close

    －免疫関連の細胞における抗生物質の代替物の生体外での検証方法の開発 －抗生物質の代替物のフィールド適用および評価

  12. 癌の転移抑制活性を有する大豆由来機能性分子の同定と作用機構の解明

    More details Close

    癌の転移抑制活性を有する大豆由来機能性分子の機能解析を行う。

  13. 発酵大豆タンパク質の機能性に関する研究

    More details Close

    豚における発酵大豆タンパク質の有効性と機能性についてIn Vivoで実験を行っている

  14. ブタの皮下脂肪厚を制御する遺伝子の解明

    More details Close

    ブタの皮下脂肪厚は枝肉価格を決定する最も重要な因子であり、価格に直結する性質である。ブタの皮下脂肪厚は遺伝的な要因と飼料等の環境要因の両者によって決定されているが、環境要因が精密に制御される近代養豚においては、遺伝的な要因の重要性がより高まっている。また消費者の要求は多様化しており、薄脂肪だけではなく味の良い厚脂肪の豚肉を好む方向も現れてきている。皮下脂肪厚の育種改良には長い期間が必要であり、従来型の育種技術では多様化した消費動向に柔軟に対応することは困難である。本課題では、消費者の要望に添った形質のブタを自由に生産する技術を開発するため、ブタの皮下脂肪厚を制御している遺伝子を特定する。

  15. Asian-Australian Journal of Animal Science 編集委員

Show all Show first 5