Details of the Researcher

PHOTO

Motoi Kikusato
Section
Graduate School of Agricultural Science
Job title
Associate Professor
Degree

  • 農学 (Tohoku University)

e-Rad No.
90613042

Research History 1

  • 2008/04 - 2011/03
    東北大学大学院農学研究科　日本学術振興会DC1特別研究員

Education 2

  • Tohoku University　Graduate School, Division of Agriculture　応用生命科学

    - 2011/03/25

  • Kagoshima University　Faculty of Agriculture　生物資源化学科

    - 2006/03/25

Committee Memberships 9

  • 日本畜産学会　Animal Science Journal編集委員（Section Editor）

    2026/04 - Present

  • Frontiers in Avian Physiology　Associate Editor

    2025/05 - Present

  • Veterinary Sciences (MDPI Journal)　Academic Editor

    2025/04 - Present

  • 日本家禽学会　The Journal of Poultry Science編集委員（Section Editor）

    2022/04 - Present

  • 日本家禽学会　評議員

    2021/04 - Present

  • Journal of Feed and Animal Sciences　Editorial Board Member (Associate Editor)

    2019/04 - Present

  • 日本家禽学会　国際交流委員

    2021/04 - 2026/03

  • 日本畜産学会　Animal Science Journal編集委員（Associate Editor）

    2020/04 - 2026/03

  • Frontiers in Physiology　Associate Editor

    2021/10 - 2024/08

Show all ︎Show first 5

Professional Memberships 5

  • 公益財団法人・腸内細菌学会

    2023/06 - Present

  • World Poultry Science Association

  • 家畜栄養生理研究会

  • 日本畜産学会

  • 日本家禽学会

Research Interests 8

  • イソフラボン

  • 炎症

  • 腸内細菌

  • Chicken

  • Polyphenols

  • Feed additives

  • Heat stress

  • Mitochondria

Research Areas 1

  • Life sciences / Animal production science /

Awards 4

  1. 日本畜産学会 Animal Science Journal Best Reviewers Awards

    2020/03　日本畜産学会

  2. Animal Science Journal Best Reviewers Awards

    2017/03　日本畜産学会

  3. Young Investigator Awards, 5th SFRR-Asia/8th ASMRM/11th J-mit International Conference

    2011/09/04　5th SFRR-Asia/8th ASMRM/11th J-mit International Conference

  4. 日本家禽学会2010年度春季学術大会 優秀発表賞

    2010/03/30　日本家禽学会　急性暑熱感作における活性酸素種（ROS）の産生増大はどんなときにおこるか？

Papers 60

  1. Equol‐Producing Bacteria in the Chicken Intestine: PCR Analysis on the Bacteria Quantitatively Correlating With Equol Concentration in Daidzein‐Fed Laying Hens Peer-reviewed

    Sota Sakai, Kyohei Furukawa, Fu Namai, Keita Nishiyama, Dipson Gyawali, Tatsuhiko Goto, Motoi Kikusato

    Animal Science Journal　2025/01

    DOI： 10.1111/asj.70068 　

  2. Effect of Feeding Sugarcane Bagasse-Extracted Polyphenolic Mixture on the Growth Performance, Meat Quality, and Oxidative and Inflammatory Status of Chronic Heat-Stressed Broiler Chickens. International-journal

    Motoi Kikusato, Fu Namai, Katsushige Yamada

    Animals : an open access journal from MDPI　14　(23)　2024/11/28

    DOI： 10.3390/ani14233443 　

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    BACKGROUND: This study aimed to evaluate the effects of sugarcane bagasse-extracted polyphenolic mixture (SBPM) supplementation on the harmful effects of chronic heat stress (HS) in broiler chickens. METHODS: Two hundred and eighty-eight day-old male Ross 308 chicks were fed an SBPM in 0, 75, 150, or 300 ppm-supplemented diets and reared under thermoneutral (TN, 22.1-24.8 °C) or chronic HS (28.3-36.2 °C) conditions from 11 d to 42 d. RESULTS: The chronic HS treatment negatively affected body weight, feed intake, and feed conversion ratio (p < 0.05), and these changes were partially attenuated by the SBPM supplementation (p < 0.05). Plasma lipid peroxidation content, inflammatory cytokines [interleukin (IL)-6, IL-β], corticosterone, and uric acid concentrations were significantly increased by HS, and these increases were attenuated by the SBPM supplementation (p < 0.05). Intestinal permeability indicator and serum fluorescein isothiocyanate-dextran levels after oral gavage were increased by HS and were also suppressed by the supplementation (p < 0.05). The HS-decreased muscle drip loss, lipid peroxidation, and glutathione content were also suppressed by the SBPM supplementation. The abovementioned alleviating effects of the SBPM were of a dose-dependent manner in most cases. CONCLUSION: This study demonstrated that SBPM supplementation can improve the growth performance, meat quality, inflammation, and intestinal permeability of chronic HS-treated broiler chickens.

  3. Suppressive Effects of β-Hydroxybutyrate Administration on Lipopolysaccharide-Induced Inflammation in Broiler Chickens. International-journal

    Tae Horiuchi, Kyohei Furukawa, Motoi Kikusato

    Veterinary sciences　11　(9)　2024/09/02

    DOI： 10.3390/vetsci11090405 　

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    BACKGROUND: This study aimed to evaluate the suppressive effects of β-hydroxybutyrate (BHB) administration on lipopolysaccharide (LPS)-induced inflammation in broiler chickens. METHODS: Twenty-day-old male broiler chickens were randomly allocated to three groups, each of which was treated with saline (control), intraperitoneal administration of LPS [1.5 mg/kg body weight (BW), Escherichia coli O127:B8], or LPS plus BHB (3 mmol/kg BW). RESULTS: Plasma albumin and total protein concentration were significantly reduced by LPS administration, while BHB co-treatment partially attenuated the effects. The LPS treatment significantly induced plasma aspartate and alanine aminotransferase activities, and interleukin (IL)-6 concentration, with the increases suppressed by BHB co-treatment (p < 0.05). The LPS treatment significantly increased the gene expression levels of IL-1β, IL-6, and IL-18 in the spleen and peripheral blood monocytes (PBMC), while the increases were partially attenuated by BHB in the spleen. Relatively higher levels of BHB dehydrogenase 1 and succinyl-CoA:3-ketoacid CoA transferase were observed in the spleen and skeletal muscle, while these gene levels were lower in PBMC and the liver. CONCLUSIONS: The present results suggest that BHB can suppress LPS-induced inflammation, in which ketolytic enzyme expression levels may be involved in broiler chickens.

  4. Mechanisms underlying the Effects of Heat Stress on Intestinal Integrity, Inflammation, and Microbiota in Chickens. International-journal Invited Peer-reviewed

    Motoi Kikusato, Masaaki Toyomizu

    The Journal of Poultry Science　60　(2)　2023021-2023021　2023/09

    DOI： 10.2141/jpsa.2023021 　

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    Poultry meat and egg production benefits from a smaller carbon footprint, as well as feed and water consumption, per unit of product, than other protein sources. Therefore, maintaining a sustainable production of poultry meat is important to meet the increasing global demand for this staple. Heat stress experienced during the summer season or in tropical/subtropical areas negatively affects the productivity and health of chickens. Crucially, its impact is predicted to grow with the acceleration of global warming. Heat stress affects the physiology, metabolism, and immune response of chickens, causing electrolyte imbalance, oxidative stress, endocrine disorders, inflammation, and immunosuppression. These changes do not occur independently, pointing to a systemic mechanism. Recently, intestinal homeostasis has been identified as an important contributor to nutrient absorption and the progression of systemic inflammation. Its mechanism of action is thought to involve neuroendocrine signaling, antioxidant response, the presence of oxidants in the diet, and microbiota composition. The present review focuses on the effect of heat stress on intestinal dysfunction in chickens and the underlying causative factors. Understanding these mechanisms will direct the design of strategies to mitigate the negative effect of heat stress, while benefiting both animal health and sustainable poultry production.

  5. Oleuropein suppresses mitochondrial reactive oxygen species generation possibly via an activation of transient receptor potential V1 and sirtuin‐1 in cultured chicken muscle cells International-journal Peer-reviewed

    Hikaru Muroi, Kouhei Hori, Yukako Tokutake, Yuki Hakamata, Fuminori Kawabata, Masaaki Toyomizu, Motoi Kikusato

    Animal Science Journal　93　(1)　e13677　2022/01

    Publisher: Wiley

    DOI： 10.1111/asj.13677 　

    ISSN： 1344-3941

    eISSN： 1740-0929

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    This study investigated the intracellular mechanism governing the effects of oleuropein (OLE), a phenolic compound of Olea europaea, on mRNA expression of avian uncoupling protein (avUCP) and mitochondrial biogenesis-related factors, and reactive oxygen species (mitROS) generation in a primary cultured chicken muscle cells. The OLE-treated cells exhibited increases in Avucp and ATP5a1z expression and a decrease in mitROS generation (p < 0.05), while the effects was canceled by sirtuin-1 (SIRT1) or transient receptor potential vanilloid 1 (TRPV1) inhibitors, EX-527 or BCTC, respectively. Intracellular Ca2+ concentration was significantly increased by OLE, while the induction was canceled by BCTC. The study also found that TRPV1 was expressed in the cell membrane and endoplasmic reticulum (ER), and Ca2+ could be released from ER in the OLE-treated cells. The OLE-treated cells exhibited increases in the phosphorylation ratio of AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) protein content. EX-527 and BCTC inhibitors canceled the effects of OLE on p-AMPK ratio and PGC-1α content, while EX-527 SIRT did not change PGC-1α content. The results suggest that the OLE effects may be due to Ca2+ release, possibly from TRPV1 at ER, and increased p-AMPK ratio, followed by SIRT1 activation and PGC-1α protein expression.

  6. In vivo emergence of beige-like fat in chickens as physiological adaptation to cold environments. International-journal Peer-reviewed

    Rina Sotome, Akira Hirasawa, Motoi Kikusato, Taku Amo, Kyohei Furukawa, Anna Kuriyagawa, Kouichi Watanabe, Anne Collin, Hitoshi Shirakawa, Ryota Hirakawa, Yuta Tanitaka, Hideki Takahashi, Guoyao Wu, Tomonori Nochi, Tsuyoshi Shimmura, Craig H Warden, Masaaki Toyomizu

    Amino Acids　53　(3)　381-393　2021/03

    DOI： 10.1007/s00726-021-02953-5 　

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    While it has been hypothesized that brown adipocytes responsible for mammalian thermogenesis are absent in birds, the existence of beige fat has yet to be studied directly. The present study tests the hypothesis that beige fat emerges in birds as a mechanism of physiological adaptation to cold environments. Subcutaneous neck adipose tissue from cold-acclimated or triiodothyronine (T3)-treated chickens exhibited increases in the expression of avian uncoupling protein (avUCP, an ortholog of mammalian UCP2 and UCP3) gene and some known mammalian beige adipocyte-specific markers. Morphological characteristics of white adipose tissues of treated chickens showed increased numbers of both small and larger clusters of multilocular fat cells within the tissues. Increases in protein levels of avUCP and mitochondrial marker protein, voltage-dependent anion channel, and immunohistochemical analysis for subcutaneous neck fat revealed the presence of potentially thermogenic mitochondria-rich cells. This is the first evidence that the capacity for thermogenesis may be acquired by differentiating adipose tissue into beige-like fat for maintaining temperature homeostasis in the subcutaneous fat 'neck warmer' in chickens exposed to a cold environment.

  7. Growth performance responses to increased tryptophan supplementation in growing barrows fed three different very low crude protein corn and soybean meal-based diets fortified with essential amino acids Peer-reviewed

    Sato, H., Miura, M., Fujieda, T., Taciak, M., Kikusato, M., Sato, K., Toyomizu, M.

    Animal Science Journal　92　(1)　2021/01

    Publisher: Wiley

    DOI： 10.1111/asj.13605 　

    ISSN： 1740-0929 1344-3941

    eISSN： 1740-0929

  8. Effect of trehalose supplementation on growth performance and intestinal morphology in broiler chickens Peer-reviewed

    Ruangpanit, Y., Matsushita, K., Mukai, K., Kikusato, M.

    Veterinary and Animal Science　10　100142-100142　2020/12

    Publisher: Elsevier BV

    DOI： 10.1016/j.vas.2020.100142 　

    ISSN： 2451-943X

  9. Differences in breast muscle mitochondrial respiratory capacity, reactive oxygen species generation, and complex characteristics between 7-week-old meat- and laying-type chickens. Peer-reviewed

    Yuki Hakamata, Masaaki Toyomizu, Motoi Kikusato

    The Journal of Poultry Science　57　(4)　319-327　2020/10/25

    DOI： 10.2141/jpsa.0190133 　

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    The skeletal muscle growth rate is a major feature differentiating meat- and laying-type chickens. A large amount of ATP is required during skeletal muscle synthesis, in which mitochondrial energy production capacities play a significant role. Additionally, mitochondria may participate in muscle protein degradation via reactive oxygen species generation. To investigate the differences in mitochondrial energetic characteristics between chickens exhibiting different growth rates, this study evaluated respiratory capacities in response to different types of respiratory substrate, protein abundances, assembly of individual respiratory complexes (I-V) and supercomplexes, and reactive oxygen species generation rates. These characteristics were compared between mitochondria from the breast muscle (M. pectoralis superficialis) of seven-week-old meat- and laying-type male chickens. Blue native polyacrylamide gel electrophoresis analysis revealed that meat-type chickens exhibited a significantly lower protein abundance of complex III (cytochrome bc1 complex), complex V (F0F1 ATP synthase), and total amount of supercomplexes than did laying-type chickens. There were no differences between chicken types in the respiration rate of mitochondria incubated with either pyruvate/malate or succinate, each of which drives complex I- and complex II-linked respiration. Carnitine palmitoyltransferase-1-dependent and -independent respiration during ATP synthesis and carnitine palmitoyltransferase-2 enzymatic activity were significantly lower in meat-type chickens than in layingtype chickens. For mitochondria receiving pyruvate/malate plus succinate, the reactive oxygen species generation rate and its ratio to the oxygen consumed (the percentage of free radical leak) were also significantly lower in meat-type chickens than in laying-type chickens. These results suggested that the mitochondrial energetic capacities of the breast muscle of meat-type chickens could be lower than those of laying-type chickens at seven weeks of age. Furthermore, the lower reactive oxygen species generation rate in meat-type chickens might have implications for rapid muscle development, which is possibly related to their lower muscle protein degradation rates.

  10. Effects of low-dose oleuropein diet supplementation on the oxidative status of skeletal muscles and plasma hormonal concentration of growing broiler chickens Peer-reviewed

    R. Shimao, H. Muroi, K. Furukawa, M. Toyomizu, M. Kikusato

    British Poultry Science　60　(6)　784-789　2019/11/02

    DOI： 10.1080/00071668.2019.1662886 　

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    © 2019, © 2019 British Poultry Science Ltd. 1. Oleuropein (Ole) is a major phenolic compound in Olea europaea, with anti-oxidative, anti-obesity, and anti-inflammatory properties. To explore the effect of Ole on the physiology and metabolism of poultry, this study, evaluated the effects of feeding low-dose Ole on the growth performance, metabolic hormonal status, muscle oxidative status in growing broiler chickens. 2. Thirty-two 8-day-old chickens were assigned to four different treatments, and fed either 0 (control), 0.1, 0.5, or 2.5 ppm Ole-supplemented diets for 2 weeks. 3. There were no differences in the body weight gain, feed consumption, and feed efficiency during the feeding periods between the groups tested. Birds fed Ole 0.5- and 2.5 ppm-supplemented diets exhibited a significant decrease in muscle carbonyl content compared to the control group. In the group fed Ole 0.5 ppm, the mRNA expression levels of mitochondrial ROS-reducing factors: avian uncoupling protein and manganese superoxide dismutase, as well as peroxisome proliferator-activated receptor γ coactivator 1-α, sirtuin-1 and −3 (each of which co-ordinately induce the transcription of the previous two factors) were upregulated compared to the control group, and the changes were independent of plasma noradrenaline and thyroid hormone levels. The group fed Ole-2.5 ppm did not show such transcriptional changes, but exhibited a higher corticosterone concentration. 4. This study demonstrates that ingesting a low dose of Ole can reduce muscle oxidative damage, and that the suppression machinery may differ depending on the amount of Ole ingested by growing broiler chickens.

  11. Differential effects of heat stress on oxidative status of skeletal muscle with different muscle fibre compositions in broiler chicken Peer-reviewed

    Kikusato, M., Toyomizu, M.

    Journal of Animal and Feed Sciences　28　(1)　78-82　2019/01/31

    Publisher: The Kielanowski Institute of Animal Physiology and Nutrition, {PAS}

    DOI： 10.22358/jafs/102830/2019 　

    ISSN： 1230-1388

  12. Characterization of mitochondrial content and respiratory capacities of broiler chicken skeletal muscles with different muscle fiber compositions. Peer-reviewed

    Yuki Hakamata, Kouichi Watanabe, Taku Amo, Masaaki Toyomizu, Motoi Kikusato

    The Journal of Poultry Science　55　(3)　210-216　2018

    DOI： 10.2141/jpsa.0170141 　

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    Mitochondrial content is regarded a useful feature to distinguish muscle-fiber types in terms of energy metabolism in skeletal muscles. Increasing evidence suggests that specific mitochondrial bioenergetic phenotypes exist in metabolically different muscle fibers. A few studies have examined the energetic properties of skeletal muscle in domestic fowls; however, no information on muscle bioenergetics in broiler chickens selectively bred for faster growth is available. In this study, we aimed to characterize the mitochondrial contents and functions of chicken skeletal muscle consisting entirely of type I (oxidative) (M. pubo-ischio-femoralis pars medialis), type IIA (glycolytic/oxidative) (M. pubo-ischio-femoralis pars lateralis), and type IIB (glycolytic) (M. pectoralis) muscle fibers. Citrate synthase (CS) activity was the highest in type IIA muscle tissues and isolated mitochondria, among the muscle tissues tested. Although no difference was registered in mitochondrial CS activity between type IIB and type I muscles, tissue CS activity was significantly higher in the latter. Histochemical staining for NADH tetrazolium reductase and the ratio of muscle-tissue to mitochondrial CS activity indicated that type I, type IIA, and type IIB muscle-fiber types showed decreasing mitochondrial content. Mitochondria from type I muscle exhibited a higher coupled respiration rate induced by pyruvate/malate, palmitoyl-CoA/malate, and palmitoyl-carnitine, as respiratory substrates, than type IIB-muscle mitochondria, while the response of mitochondria from type IIA muscle to those substrates was comparable to that of mitochondria from type I muscle. Type IIA-muscle mitochondria exhibited the highest carnitine palmitoyltransferase-2 level among all tissues tested, which may contribute to the higher fatty acid oxidation in these mitochondria. The results suggest that mitochondrial abundance is one of the features differentiating metabolic characteristics of different chicken skeletal muscle types. Moreover, the study demonstrated that type IIA-muscle mitochondria may have distinct metabolic capacities.

  13. Oleuropein induces mitochondrial biogenesis and decreases reactive oxygen species generation in cultured avian muscle cells, possibly via an up-regulation of peroxisome proliferator-activated receptor γ coactivator-1α. International-journal Peer-reviewed

    Motoi Kikusato, Hikaru Muroi, Yuichiro Uwabe, Kyohei Furukawa, Masaaki Toyomizu

    Animal science journal = Nihon chikusan Gakkaiho　87　(11)　1371-1378　2016/11

    DOI： 10.1111/asj.12559 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  14. Effects of trehalose supplementation on the growth performance and intestinal innate immunity of juvenile chicks Peer-reviewed

    M. Kikusato, F. Nanto, K. Mukai, M. Toyomizu

    British Poultry Science　57　(3)　375-380　2016/06

    DOI： 10.1080/00071668.2016.1166475 　

    ISSN： 0007-1668

    eISSN： 1466-1799

  15. Responses of Chick Myoblast Proliferation to Branched‐Chain Amino Acids in a Three‐Component System

    Kunihiko Soma, Motoi Kikusato, Kan Sato, Masaaki Toyomizu

    Animal Science Journal　2025/01

    DOI： 10.1111/asj.70106 　

  16. Effect of Bacillus hisashii Supplementation on the Growth Performance, Nitrogen Excretion, and Intestinal Microbiota of Broiler Chickens Fed Low Crude Protein Diet

    Motoi Kikusato, Fu Namai

    Animal Science Journal　2025/01

    DOI： 10.1111/asj.70088 　

  17. Trehalose Supplementation Effects on Growth, Intestinal Morphology, Gut Bacteria, and Footpad Dermatitis of Broiler Chickens Reared at High Density. International-journal International-coauthorship Peer-reviewed

    Takawan Sooksridang, Chantaluk Rachatapibul, Saksit Srinongkote, Kazuhisa Mukai, Motoi Kikusato

    The Journal of Poultry Science　61　2024001-2024001　2024

    DOI： 10.2141/jpsa.2024001 　

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    This study aimed to measure the effects of trehalose (Tre) supplementation on the growth, intestinal morphology, gut bacteria, and footpad dermatitis (FPD) of broiler chickens reared at different stocking densities (SD). Four hundred newly hatched Ross 308 male chicks were randomly allocated to four groups of eight, following a 2 × 2 factorial arrangement in a randomized complete block design using two SDs (normal, 11; high, 14 birds/m2) and two diets: basal with and without 0.5% Tre. Tre supplementation was provided during the starter/grower phase, but not the finisher phase. Data were analyzed using a two-way analysis of variance. We observed no significant effects of SD or Tre, individually or combined, on body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) during the starter/grower period. However, high SD decreased both BWG (P < 0.001) and FI (P < 0.05), and increased FCR (P < 0.001), during the finisher period. Whereas Tre reduced FCR (P < 0.05) as a main effect, no combined effect was observed on FCR. Over the total period, high SD negatively affected BWG and FCR (P < 0.001), and Tre significantly reduced FCR, with its effect unaffected by SD. No significant effects of SD or Tre were observed on jejunal morphology. The ileal abundance of Clostridium perfringens (P > 0.05) was not affected by high SD but was significantly reduced by Tre. Neither high SD nor Tre altered Lactobacillus spp. counts; however, high SD increased FPD lesion scores, whereas Tre had no effect. The study showed that Tre supplementation during the starter/grower period improved FCR during the finisher period, possibly by decreasing the abundance of C. perfringens in broiler chickens.

  18. Effect of Dietary 4-Phenylbuthyric Acid Supplementation on Acute Heat-Stress-Induced Hyperthermia in Broiler Chickens. International-journal Peer-reviewed

    Yukako Tokutake, Ryo Takanashi, Motoi Kikusato, Masaaki Toyomizu, Kan Sato

    Animals : an open access journal from MDPI　12　(16)　2022/08/12

    DOI： 10.3390/ani12162056 　

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    Hot, humid weather causes heat stress (HS) in broiler chickens, which can lead to high mortality. A recent study found that HS causes endoplasmic reticulum (ER) stress. However, the possible involvement of ER stress in HS-induced physiological alterations in broiler chickens is unclear. This study aimed to evaluate the effect of the dietary supplementation of 4-phenylbutyric acid (4-PBA), an alleviator of ER stress, in acute HS-exposed young broiler chickens. Twenty-eight 14-day-old male broiler chickens (ROSS 308) were divided into two groups and fed either a control diet or a diet containing 4-PBA (5.25 g per kg of diet feed) for 10 days. At 24 days old, each group of chickens was kept in thermoneutral (24 ± 0.5 °C) or acute HS (36 ± 0.5 °C) conditions for 2 h. The results showed that thermoneutral birds supplemented with 4-PBA exhibited no negative effects in terms of broiler body weight gain and tissue weight compared to non-supplemental birds. HS increased body temperature in both the control and 4-PBA groups, but the elevation was significantly lower in the 4-PBA group than in the control group. The plasma non-esterified fatty acid concentration was significantly increased by HS treatment in non-supplemental groups, while the increase was partially attenuated in the 4-PBA group. Moreover, 4-PBA prevented HS-induced gene elevation of the ER stress markers GRP78 and GRP94 in the skeletal muscle. These findings suggest that the 4-PBA effect may be specific to the skeletal muscle in HS-exposed birds and that 4-PBA supplementation attenuated HS-induced muscle ER stress, which could be associated with a supplementation of the body temperature elevation and lipolysis.

  19. Characteristics of Essential Oils of Apiaceae Family: Their Chemical Compositions, in vitro Properties and Effects on Broiler Production Invited Peer-reviewed

    Usman Ali, Saima Naveed, Shafqat Nawaz Qaisrani, Athar Mahmud, Zafar Hayat, Muhammad Abdullah, Motoi Kikusato, Masaaki Toyomizu

    The Journal of Poultry Science　59　(1)　16-37　2022

    Publisher: Japan Poultry Science Association

    DOI： 10.2141/jpsa.0210042 　

    ISSN： 1346-7395

    eISSN： 1349-0486

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    There has been an upsurge of interest in the phytobiotics coincident with the onset of the potential ban on the use of antibiotic growth promoters (AGPs) in the broiler industry and because many kinds of nutraceuticals play an important role in improving growth performance, feed efficiency, and gut health of broilers. In the previous years, significant biological activities of essential oils (EOs) belonging to phytobiotics were observed, including anti-bacterial, antifungal, antiviral, and antioxidant properties. We found new perspectives on the roles of EOs, particularly extracts from the Apiaceae family, which is one of the largest plant families, in potential replacement of AGPs, and on the chemical composition involved in regulating microorganism activity and oxidative damage. Furthermore, the positive effects of EOs on broiler production and the possible mechanisms inducing the involvement of gut health and growth performance have been studied.

  20. Comparative study of certain antioxidants-electrolyzed reduced water, tocotrienol and vitamin E in heat-induced oxidative damage and performance in broilers Peer-reviewed

    MAK Azad, M Kikusato, I Zulkifli, MM Rahman, MS Ali, MA Hashem, M Toyomizu

    Meat Research　1　(1)　2021/12/02

    Publisher: Bangladesh Meat Science Association

    DOI： 10.55002/mr.1.1.7 　

    eISSN： 2790-1971

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    This study was designed to examine the anti-oxidative effect of electrolyzed reduced water, tocotrienol and vitamin E on heat-induced oxidative damage and performance in an experimental model in broilers. On day 12, broiler chickens were subjected to one of the following dietary groups; (i) basal diet and untreated drinking water (control), (ii) basal diet and electrolyzed reduced drinking water (ERW), (iii) basal diet supplemented with 2% rice bran scum oil (as a source of tocotreinol) and untreated drinking water (TOCO), and (iv) basal diet supplemented with vitamin E at 50 mg/kg and untreated drinking water (VITE). On day 14, chickens were exposed to either 34°C continuously for a period of 14 days, or maintained at 24°C on the same diet. Heat-exposed birds consumed significantly less feed resulting in lower weight gain and feed efficiency compared with birds kept at 24°C. Skeletal muscle and liver MDA levels were significantly increased in heat-exposed control birds. The heat-exposed ERW chicks showed significantly improved growth performance and lower levels of MDA contents in tissues than heat-stressed control broilers. Following heat exposure, TOCO and VITE chicks did not exhibit improved performance, while those chicks significantly reduced oxidative damage to the various organs. The results demonstrate that electrolyzed reduced water, tocotrienol and vitamin E effectively protect heat-induced oxidative damage in broilers but they do not improve growth performance except electrolyzed reduced water treatment.

  21. Possible role of corticosterone in proteolysis, glycolytic, and amino acid metabolism in primary cultured avian myotubes incubated at high-temperature conditions Peer-reviewed

    Furukawa, K., Toyomizu, M., Kikusato, M.

    Domestic Animal Endocrinology　76　106608-106608　2021/07

    Publisher: Elsevier BV

    DOI： 10.1016/j.domaniend.2021.106608 　

    ISSN： 0739-7240

  22. Phytobiotics to improve health and production of broiler chickens: functions beyond the antioxidant activity International-journal Invited Peer-reviewed

    Motoi Kikusato

    Animal Bioscience　34　(3)　345-353　2021/03/01

    Publisher: Asian Australasian Association of Animal Production Societies

    DOI： 10.5713/ab.20.0842 　

    ISSN： 2765-0189

    eISSN： 2765-0235

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    Phytobiotics, also known as phytochemicals or phytogenics, have a wide variety of biological activities and have recently emerged as alternatives to synthetic antibiotic growth promoters. Numerous studies have reported the growth-promoting effects of phytobiotics in chickens, but their precise mechanism of action is yet to be elucidated. Phytobiotics are traditionally known for their antioxidant activity. However, extensive investigations have shown that these compounds also have anti-inflammatory, antimicrobial, and transcription-modulating effects. Phytobiotics are non-nutritive constituents, and their bioavailability is low. Nonetheless, their beneficial effects have been observed in several tissues or organs. The health benefits of the ingestion of phytobiotics are attributed to their antioxidant activity. However, several studies have revealed that not all these benefits could be explained by the antioxidant effects alone. In this review, I focused on the bioavailability of phytobiotics and the possible mechanisms underlying their overall effects on intestinal barrier functions, inflammatory status, gut microbiota, systemic inflammation, and metabolism, rather than the specific effects of each compound. I also discuss the possible mechanisms by which phytobiotics contribute to growth promotion in chickens.

  23. Effects of plant-derived isoquinoline alkaloids on growth performance and intestinal function of broiler chickens under heat stress Peer-reviewed

    Kikusato, M., Xue, G., Pastor, A., Niewold, T.A., Toyomizu, M.

    Poultry Science　100　(2)　957-963　2021/02

    Publisher: Elsevier BV

    DOI： 10.1016/j.psj.2020.11.050 　

    ISSN： 1525-3171 0032-5791

  24. Effect of dipeptide on intestinal peptide transporter 1 gene expression: An evaluation using primary cultured chicken intestinal epithelial cells Peer-reviewed

    Tokutake, Y., Taciak, M., Sato, K., Toyomizu, M., Kikusato, M.

    Animal Science Journal　92　(1)　2021/01

    Publisher: Wiley

    DOI： 10.1111/asj.13604 　

    ISSN： 1740-0929 1344-3941

    eISSN： 1740-0929

  25. Heat stress directly affects intestinal integrity in broiler chickens Peer-reviewed

    Fumika Nanto-Hara, Motoi Kikusato, Shyuichi Ohwada, Masaaki Toyomizu

    The Journal of Poultry Science　57　(4)　284-290　2020/10/25

    DOI： 10.2141/jpsa.0190004 　

    More details Close

    A study using pair-feeding technique was conducted to determine whether heat exposure directly or indirectly (via reduced feed intake) increases intestinal mucosal damage and permeability to endotoxin in broiler chickens. Male broiler chickens (Ross 308), 27-d-old, were subjected to one of the three treatments (n=8): 1) thermo-neutral conditions (24°C) with ad libitum feed intake, 2) heat stress conditions (33°C) with ad libitum feed intake, or 3) pair-feeding under thermo-neutral conditions, with the feed intake identical to that of heat-stressed chickens. Using these groups, two experiments were performed to evaluate temporal changes in the intestinal morphology in response to each treatment. In experiment 1, chickens were sacrificed after 24 h of exposure to the treatment conditions, while in experiment 2, chickens were sacrificed after 12 or 72 h of exposure to the treatment conditions. In experiment 1, exposure to heat stress conditions for 24 h significantly decreased both the villus height to crypt depth ratio and number of proliferating cell nuclear antigen (PCNA)-positive cells in the duodenum and increased the plasma endotoxin concentration. These findings were not observed in pair-fed chickens. In experiment 2, intestinal integrity and function were unaffected by 12 h of heat stress. On the other hand, chickens exposed to heat stress for 72 h exhibited significantly damaged intestinal morphology in the duodenum as well as increased plasma endotoxin concentration; these negative effects were not observed in pair-fed chickens. These findings suggest that the intestinal morphology and permeability changes observed in chickens that are heat-stressed for 24-72 h are due to the heat stress conditions and not due to reduced feed intake.

  26. Variation in lysine, threonine, and tryptophan availability in meat and bone meal as estimated by the slope-ratio growth assay technique in growing rats

    Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Marcin Taciak, Motoi Kikusato, Masaaki Toyomizu

    Animal Science Journal　91　(1)　2020/01/01

    Publisher: Blackwell Publishing

    DOI： 10.1111/asj.13413 　

    ISSN： 1740-0929 1344-3941

  27. Heat stress causes immune abnormalities via massive damage to effect proliferation and differentiation of lymphocytes in broiler Chickens International-journal Peer-reviewed

    Ryota Hirakawa, Siti Nurjanah, Kyohei Furukawa, Atsushi Murai, Motoi Kikusato, Tomonori Nochi, Masaaki Toyomizu

    Frontiers in Veterinary Science　7　46-46　2020

    DOI： 10.3389/fvets.2020.00046 　

    More details Close

    Broiler chickens are highly sensitive to high ambient temperatures due to their feathers, lack of skin sweat glands, and high productivity. Heat stress (HS) is a major concern for the poultry industry because it negatively affects growth as well as immune functions, which increase the potential risk of infectious disease outbreaks. Therefore, it is vital to elucidate HS's effect on the avian immune system, especially considering the global rise in average surface temperature. Our study identified a series of immunological disorders in heat-stressed broiler chickens. We exposed 22-day-old broiler chickens to a continuous HS condition (34.5 ± 0.5°C) for 14 days and immunized them with a prototype bovine serum albumin (BSA) antigen. The plasma and lymphoid tissues (thymus, bursa of Fabricius, and spleen) were harvested at the end of the experiments to investigate the induction of BSA-specific immune responses. Our results revealed that plasma titers of immunoglobulin (Ig)Y, IgM, and IgA antibodies specific for BSA were lower than those of thermoneutral chickens immunized with BSA. Furthermore, the spleens of the heat-stressed broiler chickens displayed severe depression of Bu1+ B cells and CD3+ T cells, including CD4+ T cells and CD8+ T cells, and lacked a fully developed germinal center (GC), which is crucial for B cell proliferation. These immunological abnormalities might be associated with severe depression of CD4-CD8- or CD4+CD8+ cells, which are precursors of either helper or killer T cells in the thymus and Bu1+ B cells in the bursa of Fabricius. Importantly, HS severely damaged the morphology of the thymic cortex and bursal follicles, where functional maturation of T and B cells occur. These results indicate that HS causes multiple immune abnormalities in broiler chickens by impairing the developmental process and functional maturation of T and B cells in both primary and secondary lymphoid tissues.

  28. Correlations between mitochondrial respiration activity and residual feed intake after divergent genetic selection for high and low oxygen consumption in mice Peer-reviewed

    Hongyu DARHAN, Atsushi ZODA, Motoi KIKUSATO, Masaaki TOYOMIZU, Kazuo KATOH, Sang-gun ROH, Shinichiro OGAWA, Yoshinobu UEMOTO, Masahiro SATOH, Keiichi SUZUKI

    Animal Science Journal　90　(7)　818-826　2019/04

    Publisher: Wiley

    DOI： 10.1111/asj.13210 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  29. Nutritional regulation of mitochondrial ROS production of chickens exposed to acute and chronic heat stress

    M. Toyomizu, A. Mujahid, R. Hirakawa, K. Furukawa, M. A.K. Azad, M. Taciak, M. Kikusato

    EAAP Scientific Series　138　73-81　2019

    DOI： 10.3920/978-90-8686-891-9_5 　

    ISSN： 0071-2477

  30. Selection for high and low oxygen consumption-induced differences in maintenance energy requirements of mice. International-journal Peer-reviewed

    Hongyu Darhan, Motoi Kikusato, Masaaki Toyomizu, Sang-Gun Roh, Kazuo Katoh, Masahiro Sato, Keiichi Suzuki

    Animal Science Journal　88　(7)　959-965　2017/07

    DOI： 10.1111/asj.12740 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  31. Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases. International-journal Peer-reviewed

    Tetsuro Matsuhashi, Takeya Sato, Shin-Ichiro Kanno, Takehiro Suzuki, Akihiro Matsuo, Yuki Oba, Motoi Kikusato, Emi Ogasawara, Tai Kudo, Kosuke Suzuki, Osamu Ohara, Hiroko Shimbo, Fumika Nanto, Hiroaki Yamaguchi, Daisuke Saigusa, Yasuno Mukaiyama, Akiko Watabe, Koichi Kikuchi, Hisato Shima, Eikan Mishima, Yasutoshi Akiyama, Yoshitsugu Oikawa, H O Hsin-Jung, Yukako Akiyama, Chitose Suzuki, Mitsugu Uematsu, Masaki Ogata, Naonori Kumagai, Masaaki Toyomizu, Atsushi Hozawa, Nariyasu Mano, Yuji Owada, Setsuya Aiba, Teruyuki Yanagisawa, Yoshihisa Tomioka, Shigeo Kure, Sadayoshi Ito, Kazuto Nakada, Ken-Ichiro Hayashi, Hitoshi Osaka, Takaaki Abe

    EBioMedicine　20　27-38　2017/06

    DOI： 10.1016/j.ebiom.2017.05.016 　

    More details Close

    Mitochondrial dysfunction increases oxidative stress and depletes ATP in a variety of disorders. Several antioxidant therapies and drugs affecting mitochondrial biogenesis are undergoing investigation, although not all of them have demonstrated favorable effects in the clinic. We recently reported a therapeutic mitochondrial drug mitochonic acid MA-5 (Tohoku J. Exp. Med., 2015). MA-5 increased ATP, rescued mitochondrial disease fibroblasts and prolonged the life span of the disease model "Mitomouse" (JASN, 2016). To investigate the potential of MA-5 on various mitochondrial diseases, we collected 25 cases of fibroblasts from various genetic mutations and cell protective effect of MA-5 and the ATP producing mechanism was examined. 24 out of the 25 patient fibroblasts (96%) were responded to MA-5. Under oxidative stress condition, the GDF-15 was increased and this increase was significantly abrogated by MA-5. The serum GDF-15 elevated in Mitomouse was likewise reduced by MA-5. MA-5 facilitates mitochondrial ATP production and reduces ROS independent of ETC by facilitating ATP synthase oligomerization and supercomplex formation with mitofilin/Mic60. MA-5 reduced mitochondria fragmentation, restores crista shape and dynamics. MA-5 has potential as a drug for the treatment of various mitochondrial diseases. The diagnostic use of GDF-15 will be also useful in a forthcoming MA-5 clinical trial.

  32. The suppressive effect of dietary coenzyme Q10 on mitochondrial reactive oxygen species production and oxidative stress in chickens exposed to heat stress. International-journal Peer-reviewed

    Motoi Kikusato, Kasumi Nakamura, Yukiko Mikami, Ahmad Mujahid, Masaaki Toyomizu

    Animal Science Journal　87　(10)　1244-1251　2016/10

    DOI： 10.1111/asj.12543 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  33. Mitochonic Acid 5 Binds Mitochondria and Ameliorates Renal Tubular and Cardiac Myocyte Damage. International-journal Peer-reviewed

    Takehiro Suzuki, Hiroaki Yamaguchi, Motoi Kikusato, Osamu Hashizume, Satoru Nagatoishi, Akihiro Matsuo, Takeya Sato, Tai Kudo, Tetsuro Matsuhashi, Kazutaka Murayama, Yuki Ohba, Shun Watanabe, Shin-Ichiro Kanno, Daichi Minaki, Daisuke Saigusa, Hiroko Shinbo, Nobuyoshi Mori, Akinori Yuri, Miyuki Yokoro, Eikan Mishima, Hisato Shima, Yasutoshi Akiyama, Yoichi Takeuchi, Koichi Kikuchi, Takafumi Toyohara, Chitose Suzuki, Takaharu Ichimura, Jun-Ichi Anzai, Masahiro Kohzuki, Nariyasu Mano, Shigeo Kure, Teruyuki Yanagisawa, Yoshihisa Tomioka, Masaaki Toyomizu, Kohei Tsumoto, Kazuto Nakada, Joseph V Bonventre, Sadayoshi Ito, Hitoshi Osaka, Ken-Ichi Hayashi, Takaaki Abe

    Journal of the American Society of Nephrology : JASN　27　(7)　1925-32　2016/07

    DOI： 10.1681/ASN.2015060623 　

    ISSN： 1046-6673

    eISSN： 1533-3450

  34. Time-course changes in muscle protein degradation in heat-stressed chickens: Possible involvement of corticosterone and mitochondrial reactive oxygen species generation in induction of the ubiquitin-proteasome system. International-journal Peer-reviewed

    Kyohei Furukawa, Motoi Kikusato, Tomomi Kamizono, Masaaki Toyomizu

    General and Comparative Endocrinology　228　105-110　2016/03/01

    DOI： 10.1016/j.ygcen.2016.02.007 　

    ISSN： 0016-6480

    eISSN： 1095-6840

  35. Effect of feeding diets combining whole-grain paddy rice and high levels of fat on broiler chicken growth Peer-reviewed

    Fumika Nanto, Chiaki Ito, Motoi Kikusato, Masaaki Toyomizu

    The Journal of Poultry Science　53　(1)　34-39　2016/01/25

    DOI： 10.2141/jpsa.0150070 　

    ISSN： 1346-7395

  36. Roles of mitochondrial oxidative phosphorylation and reactive oxygen species generation in the metabolic modification of avian skeletal muscle Peer-reviewed

    Kikusato M, Furukawa K, Kamizono T, Hakamata Y, Toyomizu M

    Proceedings of Japan Society of Animal Nutrition and Metabolism　60　(2)　57-68　2016

    Publisher:

    ISSN： 0286-4754

  37. Moderate dependence of reactive oxygen species production on membrane potential in avian muscle mitochondria oxidizing glycerol 3-phosphate. Peer-reviewed

    Motoi Kikusato, Masaaki Toyomizu

    The Journal of Physiological Sciences　65　(6)　555-9　2015/11

    DOI： 10.1007/s12576-015-0395-2 　

    ISSN： 1880-6546

    eISSN： 1880-6562

  38. Possible involvement of mitochondrial reactive oxygen species production in protein degradation induced by heat stress in avian muscle cells Peer-reviewed

    Kyohei Furukawa, Motoi Kikusato, Tomomi Kamizono, Hayami Yoshida, Masaaki Toyomizu

    The Journal of Poultry Science　52　(4)　260-267　2015/10/25

    DOI： 10.2141/jpsa.0150028 　

    ISSN： 1346-7395

    eISSN： 1349-0486

  39. Selection for high and low oxygen consumption altered hepatic mitochondrial energy efficiency in mice Peer-reviewed

    Yu Hong, Astrid Ardiyanti, Motoi Kikusato, Tomoyuki Shimazu, Masaaki Toyomizu, Keiichi Suzuki

    Animal Science Journal　86　(9)　818-825　2015/09

    DOI： 10.1111/asj.12366 　

    ISSN： 1344-3941

    eISSN： 1740-0929

  40. Effect of heat stress-induced production of mitochondrial reactive oxygen species on NADPH oxidase and heme oxygenase-1 mRNA levels in avian muscle cells. International-journal Peer-reviewed

    Motoi Kikusato, Hayami Yoshida, Kyohei Furukawa, Masaaki Toyomizu

    Journal of Thermal Biology　52　8-13　2015/08

    DOI： 10.1016/j.jtherbio.2015.04.005 　

    ISSN： 0306-4565

  41. Effect of feeding butoxybutyl alcohol on the growth performance and status of skeletal muscle proteolysis in broiler chickens Peer-reviewed

    T. Kamizono, D. Saputra, I. Miura, M. Kikusato, K. Hayashi, M. Toyomizu

    Journal of Agricultural Science　153　(5)　920-928　2015/07

    DOI： 10.1017/S0021859615000039 　

    ISSN： 0021-8596

    eISSN： 1469-5146

  42. Effects of Whole-Grain Paddy Rice on Growth Performance, Oxidative Stress and Morphological Alterations of the Intestine in Broiler Chickens Exposed to Acute and Chronic Heat Stress Peer-reviewed

    Fumika Nanto, Chiaki Ito, Motoi Kikusato, Masaaki Toyomizu

    The Journal of Poultry Science　52　(2)　109-118　2015/04

    DOI： 10.2141/jpsa.0140009 　

    ISSN： 1346-7395

  43. Methionine deficiency leads to hepatic fat accretion via impairment of fatty acid import by carnitine palmitoyltransferase I Peer-reviewed

    M. Kikusato, S. Sudo, M. Toyomizu

    British Poultry Science　56　(2)　225-231　2015/03

    DOI： 10.1080/00071668.2014.996529 　

    ISSN： 0007-1668

    eISSN： 1466-1799

  44. Mitochonic Acid 5 (MA-5), a Derivative of the Plant Hormone Indole-3-Acetic Acid, Improves Survival of Fibroblasts from Patients with Mitochondrial Diseases Peer-reviewed

    Takehiro Suzuki, Hiroaki Yamaguchi, Motoi Kikusato, Tetsuro Matsuhashi, Akihiro Matsuo, Takeya Sato, Yuki Oba, Shun Watanabe, Daichi Minaki, Daisuke Saigusa, Hiroko Shimbo, Nobuyoshi Mori, Eikan Mishima, Hisato Shima, Yasutoshi Akiyama, Yoichi Takeuchi, Akinori Yuri, Koichi Kikuchi, Takafumi Toyohara, Chitose Suzuki, Masahiro Kohzuki, Jun-ichi Anzai, Nariyasu Mano, Shigeo Kure, Teruyuki Yanagisawa, Yoshihisa Tomioka, Masaaki Toyomizu, Sadayoshi Ito, Hitoshi Osaka, Ken-ichiro Hayashi, Takaaki Abe

    The Tohoku Journal of Experimental Medicine　236　(3)　225-232　2015

    DOI： 10.1620/tjem.236.225 　

    ISSN： 0040-8727

    eISSN： 1349-3329

  45. Effect of paddy rice diets on intestinal morphology and inflammatory responses in broiler chickens under chronic heat stress conditions Peer-reviewed

    Nanto, F, Kikusato, M, Ohwada, S, Takahashi, K, Toyomizu, M

    The 10th Asian Pacific Poultry Conference　2014/10

  46. Oleuropein, one of polyphenols in olive oil, attenuates heat stress-induced overproduction of mitochondrial ROS in skeletal muscle of birds Peer-reviewed

    Kikusato, M, Uwabe, Y, Toyomizu, M

    14th European Poultry Conference (EPC2014)　2014/06

  47. The heat-induced production of reactive oxygen species regulates protein content in cultured chick skeletal muscle cells Peer-reviewed

    Yoshida H, Kikusato M, Souma K, Toyomizu M

    The International Symposium on Energy and Protein Metabolism and Nutrition(ISEP)　134　309-310　2013/09

  48. Heat stress-induced overproduction of mitochondrial ROS is down-regulated in laying-type chickens Peer-reviewed

    Kikusato M, Toyomizu M

    The International Symposium on Energy and Protein Metabolism and Nutrition(ISEP)　134　267-268　2013/09

  49. Electrolysed reduced water decreases reactive oxygen species-induced oxidative damage to skeletal muscle and improves performance in broiler chickens exposed to medium-term chronic heat stress Peer-reviewed

    M. A. K. Azad, M. Kikusato, I. Zulkifli, M. Toyomizu

    British Poultry Science　54　(4)　503-509　2013/08

    DOI： 10.1080/00071668.2013.801067 　

    ISSN： 0007-1668

    eISSN： 1466-1799

  50. Crucial role of membrane potential in heat stress-induced overproduction of reactive oxygen species in avian skeletal muscle mitochondria Peer-reviewed

    Motoi Kikusato, Masaaki Toyomizu

    PLoS ONE　8　(5)　e64412　2013/05/09

    DOI： 10.1371/journal.pone.0064412 　

    ISSN： 1932-6203

  51. Effects of dehulled, crushed and untreated whole-grain paddy rice on growth performance in Broiler Chickens Peer-reviewed

    Fumika Nanto, Motoi Kikusato, Chiaki Ito, Shota Sudo, Masaaki Toyomizu

    The Journal of Poultry Science　49　(4)　291-299　2012/10

    DOI： 10.2141/jpsa.0120049 　

    ISSN： 1346-7395

  52. Meat-type chickens have a higher efficiency of mitochondrial oxidative phosphorylation than laying-type chickens Peer-reviewed

    Masaaki Toyomizu, Motoi Kikusato, Yusuke Kawabata, Md. Abul Kalam Azad, Eriko Inui, Taku Amo

    Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiolog　159　(1)　75-81　2011/05

    DOI： 10.1016/j.cbpa.2011.01.020 　

    ISSN： 1095-6433

    eISSN： 1531-4332

  53. Time course of ROS production in skeletal muscle mitochondria from chronic heat-exposed broiler chicken Peer-reviewed

    M. A. K. Azad, M. Kikusato, S. Sudo, T. Amo, M. Toyomizu

    Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiolog　157　(3)　266-271　2010/11

    DOI： 10.1016/j.cbpa.2010.07.011 　

    ISSN： 1095-6433

  54. Effect of chronic heat stress on performance and oxidative damage in different strains of chickens Peer-reviewed

    Kalam M. A. Azad, Motoi Kikusato, Azharul M. Hoque, Masaaki Toyomizu

    The Journal of Poultry Science　47　(4)　333-337　2010/10

    DOI： 10.2141/jpsa.010025 　

    ISSN： 1346-7395

  55. Application of modular kinetic analysis to mitochondrial oxidative phosphorylation in skeletal muscle of birds exposed to acute heat stress Peer-reviewed

    Motoi Kikusato, A. Jon J. Ramsey, Taku Amo, Masaaki Toyomizu

    FEBS Letters　584　(14)　3143-3148　2010/07

    DOI： 10.1016/j.febslet.2010.05.057 　

    ISSN： 0014-5793

  56. Lactate and adenosine triphosphate in the extender enhance the cryosurvival of rat epididymal sperm Peer-reviewed

    Hideaki Yamashiro, Masaaki Toyomizu, Motoi Kikusato, Natsuki Toyama, Satoshi Sugimura, Yumi Hoshino, Hiroyuki Abe, Stefan Moisyadi, Eimei Sato

    The Journal of the American Association for Laboratory Animal Science　49　(2)　160-166　2010/03

    ISSN： 1559-6109

  57. Metabolic characteristics and oxidative damage to skeletal muscle in broiler chickens exposed to chronic heat stress Peer-reviewed

    Azad, M.A.K., Kikusato, M., Maekawa, T., Shirakawa, H., Toyomizu, M.

    Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology　155　(3)　401-406　2010/03

    DOI： 10.1016/j.cbpa.2009.12.011 　

    ISSN： 1531-4332 1095-6433

    eISSN： 1531-4332

  58. Mitochondrial energetics and ROS production in chicken skeletal muscle: Application of modular kinetic analysis Invited Peer-reviewed

    Toyomizu, M., Kikusato, M., Amo, T., Azad, M.A.K.

    EAAP Scientific Series　127　(1)　39-46　2010

    Publisher: EAAP Scientific Series

    ISSN： 0071-2477

  59. Mechanistic analysis of mitochondrial ROS production in chicken skeletal muscle under acute heat stress conditions Peer-reviewed

    Kikusato, M., Toyomizu, M.

    EAAP Scientific Series　127　(1)　69-70　2010

    Publisher: EAAP Scientific Series

    ISSN： 0071-2477

  60. Up-regulation or activation of avian UCP attenuates mitochondrial ROS production and oxidative damage in broiler chickens exposed to acute heat stress Peer-reviewed

    A. Mujahid, Y. Akiba, M. Kikusato, M. Toyomizu

    ENERGY AND PROTEIN METABOLISM AND NUTRITION　(124)　55-56　2007

    ISSN： 0071-2477

Show all ︎Show first 5

Misc. 49

  1. ニワトリにおけるエクオール産生菌の探索

    坂井創太, 生井楓, 古川恭平, 西山啓太, 喜久里基

    日本畜産学会大会講演要旨　132nd　2024

    ISSN： 1342-4688

  2. 加熱処理プロバイオティクスが高密度飼育時のブロイラーの飼育成績,免疫応答,腸内細菌叢におよぼす影響

    喜久里基, 古川恭平, 福井和夫

    日本家禽学会誌　60　2023

    ISSN： 0029-0254

  3. ニワトリにおけるリポポリサッカライド誘導性炎症時の3-ヒドロキシ酪酸の抗炎症作用の検証

    堀内多恵, 古川恭平, 喜久里基

    日本畜産学会大会講演要旨　131st　2023

    ISSN： 1342-4688

  4. 暑熱環境下における肉用鶏ブロイラーの脂質代謝特性の変化

    徳武優佳子, 河野涼香, 喜久里基, 佐藤幹

    日本家禽学会誌　59　2022

    ISSN： 0029-0254

  5. 小胞体ストレス緩和剤4-フェニル酪酸が急性暑熱感作後の肉用鶏の血中成分と骨格筋に及ぼす効果

    徳武優佳子, 高梨涼, 喜久里基, 豊水正昭, 佐藤幹

    日本畜産学会大会講演要旨　129th　2021

    ISSN： 1342-4688

  6. Both shochu distillery by-product, and butoxybutyl alcohol promote growth via suppression of skeletal muscle proteolysis in broiler chickens

    神園巴美, 神園巴美, 喜久里基, 林國興, 豊水正昭

    栄養生理研究会報　64　(1/2)　2020

    ISSN： 0286-4754

  7. 暑熱感作鶏の骨格筋タンパク質分解に対するグルタミン・グルタミン酸同時給与の抑制効果

    古川恭平, 喜久里基, WU Guoyao, 豊水正昭

    日本家禽学会誌　56　2019

    ISSN： 0029-0254

  8. 急性暑熱感作時における肉用鶏の小胞体ストレス応答の解析

    高梨涼, 徳武優佳子, 徳武優佳子, 古川恭平, 谷高優太, 喜久里基, 喜久里基, 豊水正昭, 豊水正昭

    日本畜産学会大会講演要旨　125th　2019

    ISSN： 1342-4688

  9. 暑熱環境下のニワトリにおける液性免疫機能低下機序の解明

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    日本畜産学会大会講演要旨　125th　2019

    ISSN： 1342-4688

  10. Heat stress-induced muscle proteolytic mechanism and the nutritional regulation in broiler chickens

    63　(1)　43-53　2019

    Publisher: 家畜栄養生理研究会

    ISSN： 0286-4754

  11. 肉用鶏へのCNSLならびに加熱CNSL給与時におけるその動態と腸内細菌叢の解析

    永津健太郎, AZAD Md. Abul Kalam, 南都文香, 古川恭平, 平川良太, 喜久里基, 陶山佳久, 森田英利, 嶋田武志, 豊水正昭

    日本家禽学会誌　55　18　2018/03/30

    ISSN： 0029-0254

  12. 暑熱環境が肉用鶏のリンパ組織に及ぼす影響

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    日本畜産学会大会講演要旨　124th　2018

    ISSN： 1342-4688

  13. 鶏における暑熱感作時の骨格筋タンパク質分解亢進の分子メカニズムの解明

    古川恭平, 喜久里基, 豊水正昭

    日本家禽学会誌　55　2018

    ISSN： 0029-0254

  14. 暑熱条件下で抗原接種した鶏の液性免疫応答が遅延する原因解明

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    日本家禽学会誌　55　2018

    ISSN： 0029-0254

  15. 飼料用米給与は暑熱感作時の肉用鶏の生産性に影響するか

    近藤あずさ, 南都文香, 南都文香, 喜久里基, 豊水正昭

    日本家禽学会誌　54　20　2017/03/30

    ISSN： 0029-0254

  16. 寒冷曝露および甲状腺ホルモン給与はニワトリ脂肪組織のベージュ化を誘導するか

    五月女里梨, 喜久里基, 南都文香, 平澤明, 豊水正昭

    日本畜産学会大会講演要旨　122nd　170　2017/03/28

    ISSN： 1342-4688

  17. 黒麹給与が肉用鶏の腸管形態および酸化ストレスに及ぼす影響

    神園巴美, 南都文香, 日置久美子, 山元正博, 喜久里基, 豊水正昭

    日本畜産学会大会講演要旨　122nd　128　2017/03/28

    ISSN： 1342-4688

  18. 熱ストレスを負荷したニワトリにおける解糖減少は免疫応答と関連している(Decreased Glycolysis Relates to Immune Response in Heat-Stressed Chicken)

    Nurjanah Siti, 野地 智法, 南都 文香, 古川 恭平, 阿部 汐里, 福田 栞, 喜久里 基, 豊水 正昭

    日本畜産学会大会講演要旨集　122回　127-127　2017/03

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  19. 生理的濃度のコルチコステロンはニワトリ高温培養筋細胞のタンパク質分解を増強しない

    古川恭平, 喜久里基, 豊水正昭

    日本畜産学会大会講演要旨　122nd　2017

    ISSN： 1342-4688

  20. 飼料用米給与時における鶏の暑熱感作応答

    南都文香, 近藤あずさ, 喜久里基, 松木悠, 陶山佳久, 森田英利, 豊水正昭

    日本畜産学会大会講演要旨　121st　143　2016/03/27

    ISSN： 1342-4688

  21. エネルギー源の異なる高代謝エネルギー(ME)飼料が肉用鶏の飼養成績および肉質におよぼす影響

    近藤あずさ, 南都文香, 喜久里基, 鈴木和明, 豊水正昭

    日本家禽学会誌大会号　52　17　2015/09/10

    ISSN： 0029-0254

  22. 籾米飼料へのトレハロース添加は慢性暑熱曝露鶏の成長と盲腸内細菌叢に影響をおよぼす

    南都文香, 喜久里基, 松木悠, 陶山佳久, 向井和久, 豊水正昭

    日本家禽学会誌大会号　52　23　2015/03/10

    ISSN： 0029-0254

  23. トレハロース給与が幼雛期の肉用鶏の飼養成績・炎症応答におよぼす影響

    喜久里基, 南都文香, 向井和久, 豊水正昭

    日本家禽学会誌大会号　52　23　2015/03/10

    ISSN： 0029-0254

  24. 高温処理時の鶏骨格筋細胞におけるミトコンドリア活性酸素種(ROS)がタンパク質分解に及ぼす影響

    古川恭平, 喜久里基, 神園巴美, 吉田隼巳, 豊水正昭

    日本畜産学会大会講演要旨　119th　2015

    ISSN： 1342-4688

  25. ニワトリ筋細胞におけるオレウロペインのPGC-1α発現誘導作用がミトコンドリア生合成・活性酸素産生におよぼす影響

    室井ひかる, 喜久里基, 上部雄一郎, 古川恭平, 豊水正昭

    日本畜産学会大会講演要旨　120th　2015

    ISSN： 1342-4688

  26. 慢性暑熱曝露時のタンパク質分解関連遺伝子発現亢進におけるミトコンドリア活性酸素種(ROS)の関与

    古川恭平, 喜久里基, 神園巴美, 豊水正昭

    日本家禽学会誌　52　2015

    ISSN： 0029-0254

  27. Mitochondrial Function and Poultry Production

    Masaaki TOYOMIZU, Motoi KIKUSATO

    The 10th Asian Pacific Poultry Conference　19-23　2014/10

  28. 暑熱曝露にともなう鶏腸管の形態損傷ならびに内毒素の血中流入は飼料摂取量低下に起因しない

    南都文香, 喜久里基, 大和田修一, 豊水正昭

    日本家禽学会誌大会号　51　25　2014/09/10

    ISSN： 0029-0254

  29. コエンザイムQ10(CoQ10)を給与した暑熱感作鶏の肝臓脂質過酸化抑制作用の解明:α‐トコフェロールとアスコルビン酸との相互作用

    三上由紀子, 喜久里基, 南都文香, 伊藤千晶, 豊水正昭

    日本家禽学会誌大会号　51　17　2014/09/10

    ISSN： 0029-0254

  30. 肉用鶏における飼料由来の放射性および非放射性セシウムの体内動態

    土井雄太, 和田道治, 木村和彦, 菅野均志, 白川仁, 喜久里基, 後藤知子, 南都文香, 豊水正昭

    日本畜産学会大会講演要旨　118th　107　2014/03/27

    ISSN： 1342-4688

  31. 高脂肪含有籾米飼料が肉用鶏の発育に及ぼす影響

    伊藤千晶, 南都文香, 神園巴美, 喜久里基, 豊水正昭

    日本畜産学会大会講演要旨　118th　107　2014/03/27

    ISSN： 1342-4688

  32. 慢性暑熱環境下における飼料用籾米飼料給与が肉用鶏腸管の形態,機能および免疫応答に与える影響

    南都文香, 喜久里基, 大和田修一, 高橋和昭, 豊水正昭

    日本家禽学会誌大会号　51　24　2014/03/10

    ISSN： 0029-0254

  33. 枯草菌添加飼料が鶏の増体量・腸管形態・腸内細菌叢に及ぼす影響

    平畝真奈美, 喜久里基, 南都文香, 芦田延久, 今林寛和, 豊水正昭

    日本家禽学会誌大会号　51　18　2014/03/10

    ISSN： 0029-0254

  34. 電解還元水給与による慢性暑熱曝露鶏の酸化ストレス緩和機構の解明ならびに炎症応答抑制効果の検証

    松野良平, 喜久里基, 古川恭平, 大津晴彦, 高橋和昭, 豊水正昭

    日本家禽学会誌　51　2014

    ISSN： 0029-0254

  35. 培養骨格筋細胞を用いた家禽の暑熱ストレス発生機序の解明 －活性酸素の過剰産生が体タンパク質分解亢進に及ぼす影響̶－

    喜久里基, 吉田隼巳, 古川恭平, 神園巴美, 豊水正昭

    家畜栄養生理研究会報　54　(57)　66　2014

  36. In vitro studies investigating a possible mechanism by which heat-induced reactive oxygen species (ROS) down-regulates the protein contents in skeletal muscle of broilers chickens

    喜久里基, 吉田隼巳, 古川恭平, 神園巴美, 豊水正昭

    家畜栄養生理研究会報　58　(2)　53-63　2014

    Publisher: 家畜栄養生理研究会

    ISSN： 0286-4754

  37. 高脂肪飼料摂取マウスにおける末梢セロトニンの褐色脂肪組織ミトコンドリア活性化作用

    齋藤遼, 渡邉一史, 中野辰也, 住吉敬介, 長澤裕哉, 喜久里基, 渡邊康一, 麻生久

    日本畜産学会大会講演要旨　116th　96-96　2013/03/28

    Publisher: (公社)日本畜産学会

    ISSN： 1342-4688

  38. 肉用鶏が放射性セシウムを摂取した時の体内蓄積について

    豊水正昭, 土井雄太, 南都文香, 喜久里基, 木島裕美, 白川仁, 和田道治

    日本畜産学会大会講演要旨　116th　128　2013/03/28

    ISSN： 1342-4688

  39. 慢性暑熱環境下におけるMEの異なる飼料用籾米飼料給与が肉用鶏の生産性に与える影響

    南都文香, 伊藤千晶, 喜久里基, 豊水正昭

    日本家禽学会誌大会号　50　15　2013/03/08

    ISSN： 0029-0254

  40. 末梢セロトニンの褐色脂肪細胞活性化作用

    齋藤遼, 渡邉一史, 中野辰也, 住吉敬介, 喜久里基, 長澤裕哉, 寺田俊介, 岡田夏実, 渡邊康一, 麻生久

    日本獣医学会学術集会講演要旨集　154th　170-170　2012/08/31

    Publisher: (公社)日本獣医学会

    ISSN： 1347-8621

  41. 非放射性セシウム添加飼料を肉用鶏に給与し続けた時の体内蓄積応答

    土井雄太, 菅野均志, 木村和彦, 後藤知子, 白川仁, 喜久里基, 南都文香, 豊水正昭

    日本家禽学会誌大会号　49　15　2012/08/20

    ISSN： 0029-0254

  42. 植物性油脂を多く含む全粒籾米飼料給与により肉用鶏の成長は低下する

    伊藤千晶, 南都文香, 神園巴美, 松枝朝子, 喜久里基, 豊水正昭

    日本家禽学会誌大会号　49　14　2012/03/10

    ISSN： 0029-0254

  43. 飼料用米給与が肉用鶏の生産性におよぼす影響

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本畜産学会大会講演要旨　114th　85　2011/08/26

    ISSN： 1342-4688

  44. 飼料用米給与が肉用鶏の生産性におよぼす影響

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本畜産学会大会講演要旨　114th　120　2011/08/26

    ISSN： 1342-4688

  45. 肉用鶏生産における急性および慢性暑熱ストレス応答に対する飼料用籾米の影響

    南都文香, 喜久里基, 神園巴美, 豊水正昭

    日本家禽学会誌大会号　48　12　2011/08/10

    ISSN： 0029-0254

  46. 肉用鶏生産における飼料用米利用の有効性

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本家禽学会誌大会号　48　18　2011/03/05

    ISSN： 0029-0254

  47. 急性暑熱にともなう鶏骨格筋におけるROS過剰産生機構の解明 -バイオエナジェティクスの観点からのアプローチ-

    喜久里基, 豊水正昭

    家畜栄養生理研究会報　54　57-66　2010/05

  48. Exogenous lactate and adenosine triphosphate in the extender regulated motility, oxidative activity and enhanced cryosurvival of rat epididymal sperm

    Yamashiro,H, Toyomizu,M, Kikusato,M, Toyama,N, Sugimura, S, Hoshino,Y, Abe,H, Moisyadi,S, Sato,E

    J. Am. Assoc. Lab. Anim. Sci.　49　(2)　1-7　2010

  49. 還元水飲水が暑熱時の鶏肉生産性および品質に及ぼす影響

    KALAM Azad Abul, 今成麻衣, 喜久里基, 藤村忍, 樺山繁, 豊水正昭

    日本家禽学会誌　45　2008

    ISSN： 0029-0254

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Books and Other Publications 8

  1. 鶏の研究「養鶏研究シリーズ：温室効果ガス排出削減と生産成績の両得を目指して② 肉用鶏銘柄鶏「はかた一番どり」における低蛋白質飼料の給与効果」

    東北大学大学院農学研究科, 喜久里基, 福岡県農林業総合試験場畜産部中小家畜チーム, 平川達也, 森美幸, 福原絵理子

    木香書房　2024/06

  2. 鶏の研究「養鶏研究シリーズ：温室効果ガス排出削減と生産成績の両得を目指して① 比内地鶏における低蛋白質飼料の給与効果」

    東北大学大学院農学研究科, 喜久里基, 秋田県畜産試験場比内地鶏研究部, 力丸宗弘, 鹿野亜海, 田澤謙, 中島二千花

    木香書房　2024/04

  3. 鶏の研究「ブロイラーの異常硬化肉の発生と栄養学的制御法に関する最新の知見」

    喜久里基

    木香書房　2024/02

  4. 鶏の研究「鳥インフルエンザと腸内細菌叢」

    喜久里基

    木香書房　2023/12

  5. 鶏の研究「養鶏飼料の抗栄養因子アラビノキシランをいかに処理するか」

    木香書房　2023/10

  6. 鶏の研究「養鶏の暑熱対策資材としてのベタインの効能」

    木香書房　2023/08

  7. 動物の栄養第2版

    唐澤豊, 菅原邦夫, 本人含む

    文英堂出版　2016/02/20

    ISBN: 9784830041303

  8. 日本飼養標準 家禽（2011年度版）

    阿部啓之, 池谷守司, 本人含む

    中央畜産会出版　2012/03/14

Show all Show first 5

Presentations 113

  1. ニワトリの成長と腸内細菌叢 Invited

    喜久里基

    東亜薬品工業マイクロライフ上市セミナー　2025/07/15

  2. 様々なストレス曝露による家畜消化管微生物叢の変化と生産成績への悪影響 Invited

    喜久里基

    日本畜産学会第132回大会 企業協賛シンポジウム　2024/09/18

  3. 加熱処理プロバイオティクスが高密度飼育時のブロイラーの飼育成績、免疫応答、腸内細菌叢におよぼす影響

    喜久里基, 古川恭平, 福井和夫

    日本家禽学会2023秋季大会　2023/09/21

  4. ニワトリにおけるリポポリサッカライド誘導性炎症時の3-ヒドロキシ酪酸の抗炎症作用の検証

    堀内多恵, 古川恭平, 喜久里基

    日本畜産学会第131回大会　2023/09/19

  5. 喜久里基 Invited

    2023/06/13

  6. ブロイラー前期へのトレハロース給与効果：高密度飼育における生産成績改善効果の検討

    Takawan Sooksridang、Chantaluk Rachatapibul, Saksit Srinongkote, 喜久里基, 向井和久

    日本家禽学会2023春季大会　2023/03/29

  7. 暑熱ブロイラーの体温上昇への血中ケトン体濃度低下の関与の可能性

    喜久里基, 谷高優太, 五十嵐大樹, 豊水正昭

    日本家禽学会2022秋季大会　2022/09/17

  8. 家禽における細菌叢研究の紹介 ～こんなことにも細菌叢って関係しているんです～ Invited

    喜久里基

    日本畜産学会第 130 回大会 関連学術集会 一般社団法人 産業動物細菌叢評価・活用機構主催シンポジウム　2022/09/14

  9. 飼料中トウモロコシ粒度が肉用鶏の成長・消化管におよぼす影響

    松橋花菜子, 渡邊康一, 豊水正昭, 喜久里基

    日本家禽学会2018年春季大会　2018/03/30

  10. 肉用鶏へのアナカルド酸給与時におけるその動態と腸内細菌叢の解析

    永津健太郎, Md. Abul Kalam Azad, 南都文香, 古川恭平, 平川良太, 喜久里基, 豊水正昭

    日本家禽学会2018年春季大会　2018/03/30

  11. Effects of oleuropein on lipopolysaccharide-induced hepatic injury in mice

    Ayaka Otani, Hitoshi Shirakawa, Tomonori Nochi, Masaaki Toyomizu, Motoi Kikusato

    日本畜産学会第124回大会　2018/03/28

  12. 暑熱環境が肉用鶏のリンパ組織に及ぼす影響

    平川良太, 野地智法, 喜久里基, 古川恭平, 村井篤嗣, 豊水正昭

    日本畜産学会第124回大会　2018/03/28

  13. Differences in mitochondrial fatty acid utilization and respiratory complex characteristics between meat-type and laying-type chickens International-presentation

    Hakamata Y, Amo T, Toyomizu M, Kikusato, M

    11th Asia Pacific Poultry Conference　2018/03/25

  14. Rice diet containing high fat and rice hull affects the growth performance or heat-exposed broiler chickens International-presentation

    Nanto F, Kikusato M, Owada S, Toyomizu M

    5th EAAP International Symposium on Energy and Protein Metabolism and Nutrition　2017/09/12

  15. 5th EAAP International Symposium on Energy and Protein Metabolism and Nutrition International-presentation

    Kamizono T, Kikusato M, Hayashi K, Toyomizu M

    EAAP International Symposium on Energy and Protein Metabolism and Nutrition　2017/09/12

  16. Roles of corticosterone and superoxide in the ubiquitin proteasome system in heat-stressed chickens International-presentation

    Furukawa K, Kikusato M, Toyomizu M

    5th EAAP International Symposium on Energy and Protein Metabolism and Nutrition　2017/09/12

  17. Comparisons of ROS homeostasis in avian skeletal muscle with different myofiber compositions International-presentation

    Kikusato M, Toyomizu M

    5th EAAP International Symposium on Energy and Protein Metabolism and Nutrition　2017/09/12

  18. Time-course of changes in muscle antioxidant peptide concentrations in heat-stressed broiler chickens International-presentation

    Furukawa K, Waki N, Kikusato M, Ashinara A, Ishida A, Nakashima K, Toyomizu M

    17th Animal Science Congress of Asian Australasian Animal Production　2017/08/22

  19. Effects of bacillary probiotic (Bacillus subtilis C-3102) in spore form on the growth performance and intestinal immunity of heat-stressed broiler

    Kikusato M, Nanto F, Ishikawa T, Ashida N, Imabayashi T, Toyomizu M

    17th Animal Science Congress of Asian Australasian Animal Production　2017/08/22

  20. Defining the mitochondrial histochemical characteristics of avian skeletal muscles with different muscle fiber type compositions

    Hakamata Y, Kikusato M, Watanabe K, Amo T, Toyomizu M

    17th Animal Science Congress of Asian Australasian Animal Production　2017/08/22

  21. Possible roles of corticosterone in proteolysis, antioxidant system, and glycolytic genes in avian skeletal muscle cells under heat stress conditions International-presentation

    Furukawa K, Kikusato M, Toyomizu M

    Tohoku Forum for Creativity Thematic Program 2017　2017/08/07

  22. Effect of chronic heat stress on splenic immunologic function and amino acid concentration in broiler chickens International-presentation

    Hirakawa R, Nurjanah S, Nochi T, Furukawa K, Kikusato M, Toyomizu M

    Tohoku Forum for Creativity Thematic Program 2017　2017/08/07

  23. Effects of oleuropein on lipopolysaccharide-induced liver injury International-presentation

    Otani A, Shirakawa H, Toyomizu M, Kikusato M

    Tohoku Forum for Creativity Thematic Program 2017　2017/08/07

  24. Association of electron transport chain supercomplexes with reactive oxygen species generation in avian skeletal muscles with different muscle fiber compositions International-presentation

    Hakamata Y, Watanabe K, Amo T, Toyomizu M, Kikusato M

    Tohoku Forum for Creativity Thematic Program 2017　2017/08/07

  25. Bioavailability of polyphenol: How do polyphenols act inside the body? International-presentation

    Tohoku Forum for Creativity Thematic Program 2017　2017/08/07

  26. Oleuropein suppresses oxidative damage in avian skeletal muscle, possibly via SIRT1 up-regulation International-presentation

    Kikusato M, Shimao R, Muroi H, Toyomizu M

    21st European Symposium on Poultry Nutrition　2017/05/08

  27. オリーブ特有フェノール化合物オレウロペインがニワトリ骨格筋の活性酸素産生におよぼす影響

    室井ひかる, 嶋尾里紗, 喜久里基, 神園巴美, 豊水正昭

    日本家禽学会2017年春季大会　2017/03/30

  28. 飼料用米給与は暑熱感作時の肉用鶏の生産性に影響するか

    近藤あずさ, 南都文香, 喜久里基, 豊水正昭

    日本家禽学会2017年春季大会　2017/03/30

  29. 酸素消費量高低選抜マウスにおける褐色脂肪組織のミトコンドリア呼吸活性

    紅玉, 田仲誠一郎, 喜久里基, 豊水正昭, 佐藤正寛, 鈴木啓一

    日本畜産学会第122回大会　2017/03/28

  30. 生理的濃度のコルチコステロンはニワトリ高温培養筋細胞のタンパク質分解を増強しない

    古川恭平, 喜久里基, 豊水正昭

    日本畜産学会第122回大会　2017/03/28

  31. 寒冷曝露および甲状腺ホルモン給与はニワトリ脂肪組織のベージュ化を誘導するか

    五月女里梨, 喜久里基, 南都文香, 平澤明, 豊水正昭

    日本畜産学会第122回大会　2017/03/28

  32. Decreased Glycolysis Relates to Immune Response in Heat-Stressed Chicken

    Nurjanah Siti, 野地智法, 南都文香, 古川恭平, 阿部汐里, 福田栞, 喜久里基, 豊水正昭

    日本畜産学会第122回大会　2017/03/28

  33. 黒麹給与が肉用鶏の腸管形態および酸化ストレスに及ぼす影響

    神園巴美, 南都文香, 日置久美子, 山元正博, 喜久里基, 豊水正昭

    日本畜産学会第122回大会　2017/03/28

  34. 家禽⽣産におけるトレハロースの利⽤

    第20 回トレハロースシンポジウム　2016/11/17

  35. 暑熱負荷鶏の浅胸筋および腓腹筋におけるミトコンドリア活性酸素産生制御の比較解析

    喜久里基, 豊水正昭

    日本家禽学会2016年春季大会　2016/03/30

  36. 筋線維型が異なるニワトリ骨格筋におけるミトコンドリア呼吸活性の差異

    袴田祐基, 喜久里基, 渡邊康一, 豊水正昭

    日本畜産学会第121回大会　2016/03/27

  37. 段階的暑熱環境下における肉用鶏の酸化ストレスに対するコエンザイムQ10（CoQ10）の抑制作用

    三上由紀子, 喜久里基, 豊水正昭

    日本畜産学会第121回大会　2016/03/27

  38. 飼料用米給与時における鶏の暑熱感作応答

    南都文香, 近藤あずさ, 喜久里基, 松木悠, 陶山佳久, 森田英利, 豊水正昭

    日本畜産学会第121回大会　2016/03/27

  39. エネルギー源の異なる高代謝エネルギー（ME）飼料が肉用鶏の飼養成績および肉質におよぼす影響

    近藤あずさ, 南都文香, 喜久里基, 豊水正昭

    日本家禽学会2015年秋季大会　2015/09/10

  40. ニワトリ筋細胞におけるオレウロペインのPGC-1α発現誘導作用がミトコンドリア生合成・活性酸素産生におよぼす影響

    室井ひかる, 喜久里基, 上部雄一郎, 古川恭平, 豊水正昭

    日本畜産学会第120回大会　2015/09/10

  41. 鶏における慢性暑熱ストレスに対する緩和飼料資材の探索:電解還元水とセレン酵母の給与効果

    松野良平, 喜久里基, 豊水正昭

    日本家禽学会2015年春季大会　2015/03/30

  42. 慢性暑熱曝露時のタンパク質分解関連遺伝子発現亢進におけるミトコンドリア活性酸素種（ROS）の関与

    古川恭平, 喜久里基, 神園巴美, 豊水正昭

    日本家禽学会2015年春季大会　2015/03/30

  43. 籾米飼料へのトレハロース添加は慢性暑熱曝露鶏の成長と盲腸内細菌叢に影響をおよぼす

    南都文香, 喜久里基, 松木悠, 陶山佳久, 向井和久, 豊水正昭

    日本家禽学会2015年春季大会　2015/03/30

  44. トレハロース給与が幼雛期の肉用鶏の飼養成績・炎症応答におよぼす影響

    喜久里基, 南都文香, 向井和久, 豊水正昭

    日本家禽学会2015年春季大会　2015/03/30

  45. 寒冷曝露下の鶏における骨格筋FNDC5/Irisin発現と脂肪組織avUCPの連関

    栗谷川あんな, 喜久里基, 白川仁, 天羽拓, 豊水正昭

    日本畜産学会第119回大会　2015/03/28

  46. 高温処理時の鶏骨格筋細胞におけるミトコンドリア活性酸素種（ROS）がタンパク質分解に及ぼす影響

    古川恭平, 喜久里基, 神園巴美, 吉田隼巳, 豊水正昭

    日本畜産学会第119回大会　2015/03/28

  47. コエンザイムQ10（CoQ10）を給与した暑熱感作鶏の肝臓脂質過酸化抑制作用の解明：α-トコフェロールとアスコルビン酸との相互作用

    三上由紀子, 喜久里基, 南都文香, 伊藤千晶, 豊水正昭

    日本家禽学会2014年春秋季大会　2014/09/27

  48. 暑熱曝露にともなう鶏腸管の形態損傷ならびに内毒素の血中流入は飼料摂取量低下に起因しない

    南都文香, 喜久里基, 大和田修一, 豊水正昭

    日本家禽学会2014年春秋季大会　2014/09/27

  49. Oleuropein, one of polyphenols in olive oil, attenuates heat stress-induced overproduction of mitochondrial reactive oxygen species in skeletal muscle of birds International-presentation

    Uwabe, Y, Toyomizu, M

    EPC2014　2014/06/23

  50. 枯草菌添加飼料が鶏の増体量・腸管形態・腸内細菌叢に及ぼす影響

    平畝真奈美, 南都文香, 芦田延久, 今林寛和, 豊水正昭

    日本家禽学会2014年春季大会　2014/03/29

  51. ブトキシブチルアルコールはブロイラーの骨格筋タンパク質代謝に影響して成長を促進する

    神園巴美, 林國興, 豊水正昭

    日本家禽学会2014年春季大会　2014/03/29

  52. オリーブオイル中の鶏骨格筋脱共役タンパク質（UCP）発現誘導因子の探索

    上部雄一郎, 豊水正昭

    日本家禽学会2014年春季大会　2014/03/29

  53. 電解還元水給与による慢性暑熱曝露鶏の酸化ストレス緩和機構の解明ならびに炎症応答抑制効果の検証

    松野良平, 喜久里基, 古川恭平, 大津晴彦, 高橋和昭, 豊水正昭

    日本家禽学会2014年春季大会　2014/03/29

  54. 慢性暑熱環境下における飼料用籾米飼料給与が肉用鶏腸管の形態、機能および免疫応答に与える影響

    南都文香, 大和田修一, 高橋和昭, 豊水正昭

    日本家禽学会2014年春季大会　2014/03/29

  55. 高脂肪含有籾米飼料が肉用鶏の発育に及ぼす影響

    伊藤千晶, 南都文香, 神園巴美, 豊水正昭

    日本畜産学会第118回大会　2014/03/27

  56. 肉用鶏における飼料由来の放射性および非放射性セシウムの体内動態

    土井雄太, 和田道治, 木村和彦, 菅野均志, 白川仁, 後藤知子, 南都文香, 豊水正昭

    日本畜産学会第118回大会　2014/03/27

  57. ピロロキノリンキノン（PQQ）の添加が単離鶏ミトコンドリアの呼吸機能に及ぼす影響

    田邉拓也, 天羽拓, 豊水正昭

    日本畜産学会第118回大会　2014/03/27

  58. Electrolyzed reduced water ameliorates heat stress-induced oxidative damage to liver, possibly via a deacetylation of superoxide dismutase 2 International-presentation

    Matsuno, R, Furukawa, K, Matsueda, T, Toyomizu, M

    SFRRI2014　2014/03/23

  59. Mitochondrial ROS production in skeletal muscle is exquisitely sensitive to the membrane potential regardless of the myofiber type compositions International-presentation

    Toyomizu, M

    Mitochondria 2013　2013/11/06

  60. Does heat-induced ROS regulate protein content in chick muscle cells? International-presentation

    Yoshida, H, Souma, K, Toyomizu, M

    ISEP2013　2013/09/09

  61. Heat stress-induced overproduction of mitochondrial ROS is down-regulated in laying-type chickens International-presentation

    Toyomizu, M

    ISEP2013　2013/09/09

  62. Effect of paddy rice diets on performance in chickens under thermoneutral and heat stress conditions International-presentation

    Nanto F, Ito C, Toyomizu, M

    ISEP2013　2013/09/09

  63. 暑熱ストレス研究の近年の動向

    平成２５年度関東甲信越地域鶏試験研究推進協議会　2013/08/08

  64. 慢性暑熱環境下におけるMEの異なる飼料用籾米飼料給与が肉用鶏の生産性に与える影響

    南都文香, 伊藤千晶, 喜久里基, 豊水正昭

    2013年度日本家禽学会春季大会　2013/03/29

  65. In vitro暑熱ストレスモデルを用いたROS過剰産生機構の解明

    吉田隼巳, 喜久里基, 相馬邦彦, 豊水正昭

    日本畜産学会第116回大会　2013/03/28

  66. ピロロキノリンキノン（PQQ）の添加が単離鶏肝臓ミトコンドリアの酸化的リン酸化能に及ぼす影響

    田邉拓也, 喜久里基, 豊水正昭

    日本畜産学会第116回大会　2013/03/28

  67. 肉用鶏の浅胸筋および腓腹筋におけるミトコンドリアROS産生応答

    喜久里基, 豊水正昭

    日本畜産学会第116回大会　2013/03/28

  68. Electrolyzed-reduced water alleviates oxidative stress induced by chronic heat exposure International-presentation

    Matsueda, T, Kikusato, M, Azad, M.A.K, Toyomizu, M, raduate School of Agricultural Science, Tohoku Universi

    INSDH2012　2012/10/16

  69. Oxidative stability of rice-based diets may affect growth performance of broiler chickens International-presentation

    Ito, C, Nanto, F, Kamizono, T, Matsueda, T, Kikusato, M, Toyomizu, M, Graduate School of Agricultural Science, Tohoku Universi

    INSDH2012　2012/10/16

  70. 非放射性セシウム添加飼料を肉用鶏に給与し続けた時の体内蓄積応答

    土井雄太, 菅野均志, 木村和彦, 後藤知子, 白川仁, 喜久里基, 南都文香, 豊水正昭, 東北大院農, 城大食

    2012年度日本家禽学会秋季大会　2012/09/13

  71. 産卵鶏は急性暑熱曝露時においても酸化ストレスは亢進しない

    喜久里基, 豊水正昭

    2012年度日本家禽学会秋季大会　2012/09/13

  72. Effect of dietary C5 Molasses (derived from Empty Fruit Bunches) on growth performance and meat quality in broilers International-presentation

    Kamizono, T, Kikusato, M, Toyomizu, M, Graduate School of Agricultural Science, Tohoku Universi

    XXIV World’s Poultry Congress　2012/08/05

  73. Effects of whole paddy rice on growth performance and oxidative stress in broiler chickens exposed to acute and chronic heat stress International-presentation

    Nanto, F, Kikusato, M, Kamizono, T, Toyomizu, M, Graduate School of Agricultural Science, Tohoku Universi

    XXIV World’s Poultry Congress　2012/08/05

  74. Dietary coenzyme Q10 suppresses heat stress-induced overproduction of ROS in skeletal muscle of birds, but not via mitochondrial membrane potential or basal proton leak International-presentation

    Kikusato, M, Nakamura, K, Toyomizu, M, Graduate School of Agricultural Science, Tohoku Universi

    XXIV World’s Poultry Congress　2012/08/05

  75. Olive oil (refined)-enriched diet upregulates avian uncoupling protein expression and proton leak in the skeletal muscle mitochondria International-presentation

    Kikusato, M, Yuichiro, U, Toyomizu, M, Graduate School of Agricultural Science, Tohoku University

    10th Congress of the International Society for the Study Fatty Acids and Lipids　2012/05/26

  76. 中山泰史・喜久里基・須藤翔太・松枝朝子・豊水正昭

    日本家禽学会2012年度春季大会　2012/03/30

  77. 植物性油脂を多く含む全粒籾米飼料給与により肉用鶏の成長は低下する

    伊藤千晶, 南都文香, 神園巴美, 松枝朝子, 喜久里基, 豊水正昭

    日本家禽学会2012年度春季大会　2012/03/30

  78. 鶏骨格筋細胞を用いた暑熱ストレスモデルの構築

    吉田隼巳, 相馬邦彦, 喜久里基, 豊水正昭

    日本畜産学会第115回大会　2012/03/28

  79. BCAAs三成分系における鶏骨格筋細胞の増殖および代謝応答

    相馬邦彦, 喜久里基, 佐藤幹, 豊水正昭

    日本畜産学会第115回大会　2012/03/28

  80. Crucial role of membrane potential in heat-stress induced overproduction of reactive oxygen species in skeletal muscle mitochondria International-presentation

    Kikusato, M, Toyomizu, M

    5th SFRR-Asia/8th ASMRM/11th J-mit International Conference　2011/08/31

  81. Modular kinetic analysis reveals that meat-type chickens have a higher efficiency of mitochondrial of mitochondrial oxidative phosphorylation than laying-type chickens International-presentation

    Amo, T, Kikusato, M, Kawabata, Y, Azad, MAK, Inui, E, Toyomizu, M

    5th SFRR-Asia/8th ASMRM/11th J-mit International Conference　2011/08/31

  82. 急性暑熱感作鶏における酸化損傷の組織特異性

    喜久里基, 豊水正昭

    日本畜産学会第114回大会　2011/08/26

  83. メチオニン欠乏時における鶏骨格筋ミトコンドリアの代謝応答

    須藤翔太, 喜久里基, 豊水正昭

    日本畜産学会第114回大会　2011/08/26

  84. 飼料用米給与が肉用鶏の生産性におよぼす影響

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本畜産学会第114回大会　2011/08/26

  85. 鶏における炎症応答に対するカシューナッツ殻油給与の影響

    鈴木脩悟, 斎藤雄介, 喜久里基, 高橋和昭, 豊水正昭

    日本畜産学会第114回大会　2011/08/26

  86. 飼料用米給与が肉用鶏の生産性におよぼす影響

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本畜産学会第114回大会　2011/08/26

  87. 慢性暑熱感作時のブロイラーにおける電解還元水および水素水による酸化ストレス緩和効果

    松枝朝子, 喜久里基, 須藤翔太, 豊水正昭

    日本家禽学会2011年度秋季大会　2011/08/24

  88. 肉用鶏生産における急性および慢性暑熱ストレス応答に対する飼料用米籾米の影響

    南都文香, 喜久里基, 神園巴美, 豊水正昭

    日本家禽学会2011年度秋季大会　2011/08/24

  89. 鶏の急性暑熱ストレスに対する脱共役タンパク質UCPの役割

    第21回東北大学大学院農学研究科先端農学セミナー　2011/06/17

  90. 肉用鶏生産における飼料用米利用の有効性

    南都文香, 喜久里基, 須藤翔太, 豊水正昭

    日本家禽学会2011年度春季大会　2011/03/28

  91. 急性暑熱時のコハク酸酸化にともなう鶏骨格筋ミトコンドリアのROS産生応答

    喜久里基, 豊水正昭

    日本畜産学会第111回大会　2010/09/28

  92. 鶏初代培養肝細胞における活性酸素種（ROS）産生に対するアナカルド酸の影響

    鈴木脩悟, 前川喬彦, 喜久里基, 豊水正昭

    日本畜産学会第111回大会　2010/09/28

  93. 急性暑熱感作鶏におけるエネルギー代謝関連遺伝子発現の網羅的解析

    喜久里基, 白川仁, 豊水正昭

    日本家禽学会2010年度秋季大会　2010/09/14

  94. 鶏骨格筋初代培養細胞の増殖に対する三成分系分岐鎖アミノ酸による栄養制御

    相馬邦彦, 喜久里基, 佐藤幹, 豊水正昭

    日本家禽学会2010年度秋季大会　2010/09/14

  95. 肉用鶏のミトコンドリア酸化的リン酸化効率は産卵鶏より良い

    豊水正昭, 喜久里基, 川端雄亮, Azad, MAK, 乾恵理子, 天羽拓

    日本家禽学会2010年度秋季大会　2010/09/14

  96. Mechanistic analysis of mitochondrial ROS production in chicken skeletal muscle under acute heat stress conditions International-presentation

    Kikusato, M, Toyomizu, M

    3rd EAAP International Symposium of Energy and Protein Metabolism and Nutrition　2010/06/06

  97. Mitochondrial energetics and ROS production in chicken skeletal muscle: application of modular kinetic analysis International-presentation

    Toyomizu, M, Kikusato, M, Amo, T, Azad, MAK

    3rd EAAP International Symposium of Energy and Protein Metabolism and Nutrition　2010/06/06

  98. 急性暑熱感作における活性酸素種（ROS）の産生増大はどんなときにおこるか？

    喜久里基, 豊水正昭

    日本家禽学会2010年度春季大会　2010/03/29

  99. 不飽和度の異なる油脂給与鶏における骨格筋における骨格筋ミトコンドリアのバイオエナジェティクス

    松本祐世, 喜久里基, 須藤翔太, 豊水正昭

    日本畜産学会第112回大会　2010/03/27

  100. メチオニン欠乏が鶏骨格筋ミトコンドリア機能に及ぼす影響

    須藤翔太, 喜久里基, 松本祐世, 高橋和昭, 豊水正昭

    日本畜産学会第112回大会　2010/03/27

  101. Depression in proton leak under heat stress stimulates mitochondrial ROS production regardless of membrane potential International-presentation

    Kikusato, M, Toyomizu, M

    1st International Conference on Lipid Hydroperoxide biology and medicine　2009/11/04

  102. Time course of ROS production in skeletal muscle mitochondria from chronic heat-exposed broiler chickens International-presentation

    Azad, MAK, Kikusato, M, Matsumoto, Y, Toyomizu, M

    1st International Conference on Lipid Hydroperoxide biology and medicine　2009/11/04

  103. Anacardic acid attenuates TNFα/IFNγ-stimulated ROS production in chicken hepatocytes, probably via mild uncoupling action International-presentation

    Suzuki, S, Kikusato, M, Maekawa, T, Takahashi, K, Toyomizu, M

    1st International Conference on Lipid Hydroperoxide biology and medicine　2009/11/04

  104. 急性暑熱曝露にともなう鶏骨格筋ミトコンドリアのプロトリーク応答

    喜久里基, 乾恵理子, 豊水正昭

    日本畜産学会第110回大会　2009/03/27

  105. 低温環境馴化時における鶏脱共役タンパク質avUCPの発現応答とその機能の解明

    乾恵理子, 喜久里基, 松本祐世, 豊水正昭

    日本畜産学会第110回大会　2009/03/27

  106. 高温環境下における家禽ミトコンドリアの代謝応答

    東北大学国際高等融合領域研究所 研究討論会　2009/01/28

  107. 急性暑熱ストレスにともなう鶏骨格筋ミトコンドリアROS過剰産生の代謝解析

    喜久里基, 天羽拓, 豊水正昭

    日本酸化ストレス学会第61回大会　2008/06/19

  108. Effect of chronic heat stress on performance and oxidative damage in different strains of chicken

    Azad, MAK, Kikusato, M, Mujahid, A, Toyomizu, M

    日本家禽学会2008年度春季大会　2008/03/28

  109. 還元水飲水が暑熱時の鶏肉生産性および品質に及ぼす影響

    Azad, MAK, 今成麻衣, 喜久里基, 藤村忍, 樺山繁, 豊水正昭

    日本家禽学会2008年度春季大会　2008/03/28

  110. 急性暑熱曝露下における鶏骨格筋ミトコンドリアの膜電位特性のTop-downアプローチによる解析

    喜久里基, 天羽拓, Ramsey, JJ, 豊水正昭

    日本畜産学会第109回大会　2008/03/27

  111. Up-regulation or activation of avian UCP attenuates mitochondrial ROS production and oxidation damage in broiler chickens exposed to acute heat stress International-presentation

    Mujahid, A, Akiba, Y, Kikusato, M, Toyomizu, M

    2nd EAAP International Symposium of Energy and Protein Metabolism and Nutrition　2007/09/09

  112. 急性暑熱（38°C）曝露した肉用鶏の直腸温ならびに血漿・肝臓・骨格筋のMDAに対する絶食とCTCの影響

    喜久里基, 林國興, Mujahid, A, 秋葉征夫, 豊水正昭

    日本畜産学会第107回大会　2007/03/27

  113. Adrenal hormones play important roles to tolerant acute heat stress in broilers International-presentation

    Kikusato, M, Kato, N, Ohtsuka, A, Hayashi, K

    Poultry Science Association 95th Annual Meeting　2006/06/16

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Research Projects 13

  1. リガンド依存性転写因子による骨格筋細胞の分子制御―効率的鶏肉生産の基盤－

    佐藤 幹, 米倉 真一, 喜久里 基, 徳武 優佳子

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2023/04/01 - 2026/03/31

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    Liver X receptor（LXR）やFarnesoid X receptor（FXR）などのリガンド依存性転写因子が骨格筋の代謝に関与していることがマウスなどで明らかとなっている。LXRやFXRのリガンドである胆汁酸や酸化コレステロールは、ヒナの初生期に急増することを考えると、初生期の骨格筋細胞の増殖・分化がリガンド依存性転写因子による調節作用を強く受けているものと推定される。本研究では、LXR、FXRをはじめとする一連の転写因子と、これらの転写因子の機能発現を制御するインスリンシグナルに着目して、家禽骨格筋細胞の増殖・分化に対するこれらの転写因子の作用とメカニズムを解明し、「栄養素（リガンド）による転写活性の制御→初生期の骨格筋細胞増殖促進→食肉の効率的生産」という新たな畜産の技術基盤を構築することを目的として行う。 事業初年度である本年度は、ニワトリ胚から骨格筋細胞を調整し、ニワトリ骨格筋の分化を経時的に観察する培養条件を用いて、LXR、FXR、およびSREBPsの発現変動を調べた。その結果、ニワトリ骨格筋細胞では、分化が進行するに伴いLXRおよびSREBP2の発現が上昇し、コレステロールの代謝が活性化している可能性が示唆された。一方で、FXRやSREBP1の発現は非常に低く、その発現の変動も認められなかった。そこで、LXRのアゴニストであるT0901317およびFXRのアゴニストであるGW4064を培地に添加して、細胞の増殖および分化に対する影響を観察したところ、T0901317の添加により、骨格筋細胞の分化を明確に抑制し、分化マーカーであるMyogeninの発現が有意に低下するとともに細胞の融合が抑制された。よって、ニワトリの骨格筋細胞の分化は、LXRにより制御されている可能性が強く示唆された。

  2. Study on the possible involvment of mitochondrial sulfar respiration on chicken embryo development

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2021/07 - 2024/03

  3. 腸管を基軸とした幼若雛の健全育成を支えるニワトリ粘膜免疫学の創成

    野地 智法, イスラム ジャヒドゥル, 佐藤 幹, 喜久里 基, 陶山 佳久, 松尾 歩

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(萌芽)

    Institution: 東北大学

    2021/07/09 - 2023/03/31

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    ニワトリ（鶏）の免疫学に関する教科書には、骨髄で造血幹細胞から分化したB細胞前駆細胞は、排泄腔近傍に存在するファブリキウス嚢でB細胞へと分化し、その後、脾臓やパイエル板（回腸終末部）、盲腸扁桃（盲腸基部）といった免疫臓器で機能すると記載されている（Avian Immunology 2nd Edition）。一方で、我々は、広大な表面積を有する盲腸にも成熟したB細胞が多数集積する濾胞構造が広範囲に発達していることを見出し、盲腸扁桃やパイエル板とは異なる、腸管に発達する新たな免疫臓器（Cecal patches: CP）の存在を明らかにしてきた。加えて、経時的に実施した形態学的解析を通して、CPは盲腸扁桃と同様に孵化後に形態形成されること、腸管の免疫機能に深く関わるIgA（免疫グロブリンのサブクラスの１つ）を産生する形質細胞へのB細胞からの最終分化が、盲腸扁桃やパイエル板以上に、CPで盛んに行われていることを見出し、既存の教科書には記載されていない、孵化後の鶏の腸管内で発達するCPを中心とした粘膜免疫システムの存在を提唱してきた。 <BR> そこで本研究では、孵化1, 2, 3, 5, 7 週間後の肉用鶏（Ross308系）より、盲腸を採材・固定し、CPの形態形成を組織学的に解析した。また、組織切片をIgM, IgA, IgYをマーカーとした免疫組織化学染色に供することで、抗体産生細胞の局在変化も比較解析した。その結果、CP形成が開始される時期は、盲腸扁桃の発達時期よりも少し遅く、孵化2週齢以降であることが明らかになった。このことは、盲腸扁桃とCPの形態形成を促す刺激は異なっている可能性を強く示唆していた。さらには、CPで濾胞構造が発達する時期は3週齢前後であり、同時期に濾胞内でB細胞からIgA産生細胞への最終分化が起きていることも明らかになった。

  4. Study on anorexia in heat-stressed broiler chickens: possible effects due to intestinal barrier dysfunction

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2020/04 - 2023/03

  5. Study on skeletal muscle proteolysis due to systemic metabolic dysfunction in broiler chickens exposed to heat stress Competitive

    Kikusato Motoi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)

    Category: Grant-in-Aid for Young Scientists (A)

    Institution: Tohoku University

    2016/04 - 2020/03

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    This study was conducted to elucidate trans-organ metabolic network that induces muscle protein degradation in broiler chickens exposed to heat stress (HS). In the study, corticosterone (CORT), mitochondrial reactive oxygen species generation (mitROS), intestinal barrier function, inflammatory response, amino acid metabolism were evaluated. As a result, the study revealed that i) HS-induced muscle proteolysis could be attributed to mitROS rather than CORT, and that ii) LPS influx evoked by intestinal barrier dysfunction may stimulate 3-MH and CORT secretion, and iii) Arg generated by the muscle proteolysis might contribute to the amelioration of inflammation in HS-exposed broiler chickens. These findings suggest that HS-induced muscle proteolysis may occur in the systemic inflammation in broiler chickens.

  6. Characterization of thermo-tolerance of chickens that obtain from heat-exposed breeding hens and embryos

    Kikusato Motoi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)

    Institution: Tohoku University

    2018 - 2020

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    Heat stress (HS) negatively affects the growth performance of broiler chickens; however, the underlying mechanism remains unclear. In this project, we investigated to characterize HS-induced metabolic dysregulation and the positive responses to attenuate the HS effects in chickens. Embryos obtained from breeding hens reared in summer or autumn were hatched, and chickens were reared under the same atmospheric conditions for three weeks. HS treatment increased body temperature, plasma corticosterone, uric acid, interleukin-6 content in chickens from autumn-embryo, while these elevations were not observed in chicken from summer-embryo. Metabolomic analysis revealed that plasma and hepatic 3-hydroxy butyrate levels, which is an anti-inflammatory metabolite, were reduced by HS in chickens from autumn-embryo, while it did not occur in chickens from summer-embryo. These results suggest that anti-inflammatory capacity could be associated with the heat tolerance ability of broiler chickens.

  7. Improving the tolerance to heat stress in animals through integrating control of host and gut microbiota with improved feed ingredients

    TOYOMIZU Masaaki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2015/04 - 2018/03

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    The objectives of this research are to clarify the mechanism underlying decreasing body weight and feed efficiency in broiler chickens exposed to heat stress, and to develop feed ingredients to improve the reduced performance. Heat exposure to chickens was found to induce increased proteolysis of skeletal muscles via more mitochondrial ROS generation and decreased CD3-positive cell in the spleen so that metabolic and immune systems remarkably enhance energy requirement to maintain homeostasis. Streptococcus appears to be the less dominant genus in the ileum of heat-stressed chickens. Feeding diets supplemented with cashew nut shell liquid (CNSL) causes broiler chicken to alleviate reductions of body weight and feed intake when exposed to heat, partially via control of intestinal immune-related gene expression.

  8. 新規未利用飼料資材によるストレス環境下における安定的肉用鶏生産技術の開発 Competitive

    System: Contract Research

    2013/04 - 2017/03

  9. 飼料用米の給与による畜産物の差別化技術及び家畜の健全性向上技術の開発 Competitive

    System: The Other Research Programs

    2015/04 - 2016/03

  10. Integrated control to improve animal performance under environmental stress conditions: interactions between oxidative stress and metabolic and immunological responses Competitive

    TOYOMIZU Masaaki, TAKAHASHI Kazuaki, OHTSU Haruhiko, KIKUSATO Motoi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2012/04/01 - 2015/03/31

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    The present study was conducted to investigate the effect of heat stress (HS) and endotoxin stress on redox and metabolic states of broiler chickens. HS induced oxidative stress in skeletal muscle and liver. The anti-oxidant responses in the muscle were observed, but not in the liver. The treatments of birds with lipopolysaccharide did not induce oxidative damages to those tissues, while expression of inflammatory cytokines was enhanced. Then, electrolyzed reduced water and organic selenium were given HS-birds to aim at alleviating the oxidative stress and growth retardation. As results, oxidative stress was basically restored to normal values, whereas the growth in HS conditions was not improved by both treatments. Taken together, these findings suggest that not only regulation of oxidative stress but also optimization of other physiological alteration such as intestinal microbiota may be required in order to suppress the growth retardation due to HS conditions.

  11. Interaction of mitochondrial reactive oxygen species production with protein degradation in skeletal muscle of birds exposed to heat stress Competitive

    KIKUSATO Motoi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

    Category: Grant-in-Aid for Young Scientists (B)

    Institution: Tohoku University

    2013/04 - 2015/03

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    The present study investigated the effect of mitochondrial reactive oxygen species (mtROS) production on protein degradation in skeletal muscle of birds exposed to heat stress. Heat stress resulted in simultaneous increases in mtROS production and ubiquitin ligase (atrogin-1) mRNA levels in the skeletal muscle tissues, which suggests an involvement of mtROS production in a transcription of atrogin-1 in these tissues. In avian muscle cells incubated in heat-stressed conditions, the simultaneous increases in mtROS and the ubiquitin ligase mRNA level were observed, and following the addition of Tempol into these cells not only mtROS production but also atrogin-1 mRNA level were restored to near-normal values. We here shows that mtROS production may play an important role in inducing protein degradation probably via an up-regulation of protein ubiquitination in avian skeletal muscle under heat stress conditions.

  12. Study on the role of avian uncoupling protein in heat stress-induced overproduction of mitochondrial reactive oxygen species and the regulation mechanism of the expression in birds

    KIKUSATO Motoi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up

    Category: Grant-in-Aid for Research Activity Start-up

    Institution: Tohoku University

    2011 - 2012

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    On exposure of meat-type chickens to heat stress, avian uncoupling protein (avUCP) expression in the skeletal muscle was decreased, which was involved in the overproduction of mitochondrial reactive oxygen species production (ROS) probably via an increase in the mitochondrial membrane potential. This study demonstrates that the heat stress-induced overproduction of mitochondrial ROS did not occur in the muscle mitochondria of laying-type chickens, whose avUCP expression is relatively higher than that of meat-type ones, and in mitochondria of meat-type chickens whose avUCP expression was up-regulated with dietary intake of olive oil and oleuropein. From these results, it is conceivable that avUCP expression may play an important role in suppressing the overproduction of mitochondrial ROS and oxidative damage caused by heat exposure.

  13. 暑熱ストレスが引き起こすROS過剰産生機構の解明とその制御法の開発

    喜久里 基

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 東北大学

    2008 - 2010

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    夏季などの暑熱環境下において、鶏は「酸化ストレス」状態に陥る。これにより、生産性が著しく低下すると考えられるが、酸化ストレスの発現機序は未だ明らかでなく、また、有効的な飼養技術も確立されていない。本研究では、急性暑熱時の酸化ストレス誘導因子であるミトコンドリア活性酸素種(mitROS)の過剰産生機構を明らかにするとともに、酸化ストレスを低減する機能性栄養資材を探索することを目的とする。本年度は、後者すなわち機能性資材コエンザイムQ_<10>(CoQ_<10>)を給与し、暑熱時の酸化ストレスに対する緩和効果を調べた。 CoQ_<10>は、ROSを捕捉する抗酸化物質であり、ミトコンドリア電子伝達鎖の構成因子でもある。このため、CoQ_<10>給与により、抗酸化のみならずmitROS過剰産生抑制の面からも酸化ストレス低減効果が期待できる。実験では、CoQ_<10>添加飼料(40mg/kg飼料)を10日齢より11日間、雄肉用鶏に自由摂取させた後、25℃を対照区として34℃、12時間急性暑熱曝露した(暑熱区)。酸化ストレスマーカーであるマロンジアルデヒド(MDA)およびカルボニル化タンパク質含量を調べた結果、暑熱曝露によって、血漿MDA含量ならびに浅胸筋カルボニル化タンパク質含量は、通常飼料区では有意に増加したが、CoQ_<10>飼料区ではほとんど変化しなかった。血漿中の総抗酸化能は、暑熱感作およびCoQ_<10>給与のいずれによってもほとんど変化しなかった。これに対し、浅胸筋のmitROS産生量は、FADH_2供給時において暑熱によって著しく増加したが、CoQ_<10>給与によりこの増加は抑えられた。以上の結果より、急性暑熱時において、CoQ_<10>は鶏骨格筋mitROS過剰産生を抑制し、酸化ストレスを低減することが実証された。したがって、CoQ_<10>は暑熱時の酸化ストレス緩和において有効な栄養資材になり得る可能性が示された。

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Social Activities 1

  1. 出張講義

    2016/05/13 -

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    仙台市泉高校において、畜産学の出張講義を行った。

Other 5

  1. レシチンが肉用鶏の飼養成績におよぼす影響

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    飼料に脂質レチシンを加えた際における肉用鶏の飼養成績および代謝試験成績（脂肪消化率、粗タンパク質消化率）におよぼす影響を調べた。

  2. 植物抽出物が暑熱ニワトリの飼養成績におよぼす影響

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    植物抽出物が暑熱ニワトリの飼養成績や酸化ストレスにおよぼす影響を調べた。

  3. 枯草菌給与が鶏肉品質におよぼす影響

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    枯草菌（カルスポリン）給与が鶏肉品質（酸化損傷度、ビタミンE含量、水分保持力）におよぼす影響を調べた。

  4. 飼料用米の給与による畜産物の差別化技術及び家畜の健全性向上技術の開発

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    国内自給率を目的とし、飼料用米の給与による畜産物の差別化技術及び家畜の健全性向上技術の開発を行う。

  5. 温暖化の進行に適応する畜産の生産安定技術の開発 肉用鶏での栄養管理に基づく安定生産のための暑熱対策技術の研究開発

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    暑熱環境下における家畜の生体応答を把握し、現場レベルでの生産性改善方法を模索する。