Details of the Researcher

PHOTO

Hiroyuki Hattori
Section
Graduate School of Agricultural Science
Job title
Assistant Professor

Research History 5

  • 2023/04 - Present
    Tohoku University　Graduate School of Agricultural Science

  • 2020/04 - 2023/03
    Nagoya University　Graduate School of Bioagricultural Sciences

  • 2020/04 - 2023/03
    Nagoya University　Asian Satellite Campuses Institute and Graduate School of Bioagricultural Sciences

  • 2019/04 - 2020/03
    Japan Society for the Promotion of Science

  • 2018/03 - 2019/03
    University of Alberta　Faculty of Science, Department of Chemistry

Education 3

  • Gifu University　The United Graduate School of Agricultural Science

    2017/04 - 2020/03

  • 岐阜大学大学院　応用生物科学研究科　応用生命科学専攻

    2015/04 - 2017/03

  • Gifu University　Faculty of Applied Biological Sciences　Molecular Life Science, The Food and Life Science program

    2011/04 - 2015/03

Professional Memberships 3

  • 日本農芸化学会

    2021/03 - Present

  • 日本木材学会

    2015 - Present

  • 日本生薬学会

    2015 - Present

Research Interests 1

  • 香辛料，天然物化学，食品機能化学，ケミカルバイオロジー

Research Areas 1

  • Life sciences / Bioorganic chemistry /

Awards 5

  1. 日本栄養・食糧学会 東北支部 若手奨励賞

    2025/10　日本栄養・食糧学会　ヨーロッパイエコオロギ抽出物の抗炎症作用と機能性成分の解析

  2. 学長表彰

    2020/03　岐阜大学

  3. 研究科長表彰

    2020/03　岐阜大学大学院連合農学研究科

  4. 優秀発表賞

    2016/10　2016年度日本木材学会中部支部大会　ホワイトサイプレス材精油香気成分の肥満抑制効果について

  5. Best Presentation Award

    2016/08　5th UGS5th UGSAS-GU & BWEL JISAES　Anti-obese effect of Grains of Paradise (GP) extract

Papers 19

  1. Adductome-Based Identification of Novel Oxidized Phospholipid-Lysine Adducts Formed in Low-Density Lipoprotein. International-journal Peer-reviewed

    Fumie Nakashima, Yuto Otsuka, Takuo Aoyama, Naoya Hirade, Haruka Sei, Hiroyuki Hattori, Ryosuke Kikuchi, Tadashi Matsushita, Ken-Ichi Yamada, Koji Uchida, Takahiro Shibata

    Biochemistry　65　(17)　2689-2698　2026/09/01

    DOI： 10.1021/acs.biochem.6c00425 　

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    The oxidative modification of low-density lipoprotein (LDL) is involved in the generation of lipid peroxidation-derived electrophilic aldehydes, which covalently react with apolipoprotein B-100 to form various adducts. Although the oxidative modification of LDL is critical in the pathogenesis of atherosclerosis, the structural properties of adducts in oxidized LDL (oxLDL) remain unclear. Thus, we aimed to characterize oxLDL using the adductome approach, a comprehensive mass spectrometry-based analysis designed to detect specific product ions from positively ionized oxidized phosphatidylcholine (oxPC) adducts. The amounts of several adducts, including four major oxPC-lysine (oxPC-Lys) adducts, prominently increased when LDL was oxidized with Cu2+. Analysis of the synthetic adduct candidates through mass spectrometry revealed two oxPC-Lys Schiff base adducts and two novel oxPC-Lys amide-type adducts as the major lysine adducts in oxLDL. The oxPC-Lys Schiff base adducts could be transformed into stable amide-type adducts during LDL oxidation. The amounts of oxPC-Lys adducts were significantly higher in the sera of hyperlipidemic mice than in the sera of control mice. The recognition of oxPC-Lys adducts by macrophages suggested that these adducts were involved in the phagocytosis of oxLDL by macrophages. These findings provide insights into the structural properties of lipoproteins modified under oxidative stress and their biological implications. Moreover, this study is the first to identify and quantify oxPC-Lys amide-type adducts in vitro and in vivo.

  2. Effects of γ-aminobutyric acid-rich fermented rice bran on hepatic histology, gut microbiota, and fecal metabolites in a rat model of high-fat diet-induced NAFLD-like hepatic injury. International-journal Peer-reviewed

    Yuh-Ting Huang, Lu-Chi Fu, Yi-Hsiu Chen, Qian Xiao, Hitoshi Shirakawa, Takuya Koseki, Akira Oikawa, Afifah Zahra Agista, Hiroyuki Hattori, Yusuke Ohsaki, Masako Toda, Suh-Ching Yang

    Food & function　2026/08/30

    DOI： 10.1039/d6fo02614a 　

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    γ-Aminobutyric acid (GABA)-rich fermented rice bran (GFRB) is a multi-component fermented food matrix with potential metabolic benefits, but its effects on high-fat diet (HFD)-induced liver injury and associated gut microbial and metabolic changes remain unclear. In this study, we characterized the metabolite profile of GFRB and evaluated its effects in rats with HFD-induced liver injury. Male Sprague-Dawley rats were fed a control diet or HFD with or without GABA or low- or high-dose GFRB supplementation for 12 weeks. Gut microbiota, fecal short-chain fatty acids, and fecal metabolomic profiles were further analyzed as exploratory outcomes. GFRB contained markedly increased GABA and was enriched in amino acid- and fermentation-related metabolites, including glutamine, ornithine, citrulline, lactate, and succinate. HFD feeding induced a relatively mild non-alcoholic fatty liver disease-like phenotype characterized by hepatic histopathological alterations, including fatty changes and inflammation. GFRB supplementation attenuated these hepatic alterations, as reflected by a reduced NAFLD activity score in the low-dose GFRB group and decreased hepatic interleukin-6 levels in the high-dose GFRB group. Exploratory analyses also identified alterations in gut microbiota composition and fecal metabolite profiles, particularly in amino acid- and nitrogen-related metabolic pathways. Overall, these findings suggest that GFRB may attenuate early HFD-induced liver injury, potentially through modulation of hepatic inflammation. The accompanying gut microbiota and metabolomic changes should be considered exploratory observations that warrant further mechanistic investigation.

  3. Long-term antibiotic treatment attenuates the development of food allergy in a murine model. International-journal Peer-reviewed

    Yingnan Guo, Junjie Li, Shunsuke Nishio, Hiroyuki Hattori, Tomonori Nochi, Tsukasa Matsuda, Masako Toda

    Bioscience, biotechnology, and biochemistry　2026/05/13

    DOI： 10.1093/bbb/zbag069 　

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    While short-term antibiotic exposure has been reported to exacerbate the development of allergies, the effects of long-term administration remain less understood. This study addressed the influences of prolonged antibiotic administration in a murine model of food allergy. Mice received a broad-spectrum antibiotic cocktail (ABX) for six weeks from pre-sensitization through allergen challenge. ABX reduced allergic reactions, serum IgE levels, and intestinal mast cell counts. Production of Th2 cytokine, but not of Th1 and Th17 cytokines, was lowered in allergen-stimulated splenocytes of ABX-treated allergic mice, whereas the levels of these cytokines in their intestines decreased. ABX did not significantly alter the levels of regulatory cytokine IL-10 in intestines and regulatory T cells in spleens. ABX reduced gut bacterial amount, though Lactobacillus, Lactococcus, and Streptococcus remained detectable. Collectively, the long-term ABX suppresses allergic reaction by modulating Th2-mediated events dominantly, providing insights into the complex role of antibiotics in allergy models.

  4. Structural and Functional Basis of G Protein-Coupled Receptor 35 Activation by Pyrroloquinoline Quinone. International-journal Peer-reviewed

    Harumi Ando, Akari Kanamori, Mayuka Wakabayashi, Fumie Nakashima, Haruka Sei, Hiroyuki Hattori, Masaki Kita, Asuka Inoue, Ayana Murakami, Mitsugu Akagawa, Koji Uchida, Takahiro Shibata

    Biochemistry　64　(24)　4758-4767　2025/12/16

    DOI： 10.1021/acs.biochem.5c00566 　

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    Pyrroloquinoline quinone (PQQ), an o-quinone-type nutrient, has been shown to exert diverse beneficial effects on the biochemical and physiological processes of mammals. However, the molecular mechanisms underlying these effects remain incompletely understood. Here, through screening of metabolite-sensing G protein-coupled receptors (GPCRs)─which respond to metabolites produced by gut microbiota or derived from nutrients─we found that PQQ selectively activates G-protein-coupled receptor 35 (GPR35) and characterized the molecular basis of its ligand recognition. Using a transforming growth factor α shedding assay, we demonstrated that PQQ selectively activated GPR35, a class A rhodopsin-like GPCR expressed in various tissues, including adipose tissue and the gastrointestinal tract. PQQ also promoted β-arrestin 1 recruitment to the plasma membrane, further supporting its role as a GPR35 agonist. Direct binding of PQQ to GPR35 was demonstrated using a clickable photoaffinity probe derived from PQQ. Molecular docking simulation and site-directed mutagenesis revealed that the 2-carboxylic acid moiety of PQQ forms critical hydrogen bonds with Arg100, Tyr101, and Arg151 of GPR35. Additionally, Phe163 appears to contribute to π-H interaction with PQQ. These findings indicate that PQQ functions as a food-derived agonist of GPR35 and provide new insights into the molecular mechanisms underlying the potential beneficial effects of PQQ.

  5. Dietary anthocyanidin pelargonidin activates G protein-coupled receptor 35. International-journal Peer-reviewed

    Fumie Nakashima, Sayako Shimomura, Mayuka Wakabayashi, Wei Qi Loh, Harumi Ando, Haruka Sei, Hiroyuki Hattori, Didik Huswo Utomo, Masaki Kita, Asuka Inoue, Koji Uchida, Takahiro Shibata

    The FEBS journal　2025/10/29

    DOI： 10.1111/febs.70311 　

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    G protein-coupled receptors (GPCRs) are the largest superfamily of cell surface receptors. They regulate critical physiological events and serve as potential therapeutic targets. G protein-coupled receptor 35 (GPR35), a class A rhodopsin-like GPCR expressed in various tissues, including adipose tissue and the gastrointestinal tract, has roles in diverse functions, including antioxidant, anticarcinogenic, and anti-inflammatory effects. Although many endogenous and synthetic GPR35 agonists have been identified, the understanding of food-derived agonists is limited. In this study, we discovered pelargonidin as a newly identified food-derived GPR35 agonist through a systematic screening approach. We evaluated 28 dietary phytochemicals using a transforming growth factor α (TGFα) shedding assay to evaluate GPR35 activation, and found that cyanidin, a common 3-hydroxyanthocyanidin present in various red fruits and vegetables, induced GPR35 activation. Among a series of 3-hydroxyanthocyanidins tested, pelargonidin, characterized by its monohydroxylated B-ring, exhibited the most potent agonistic activity. Mutational studies demonstrated that the hydrogen bond between the 3-hydroxy group in the C-ring of pelargonidin and Asn169, as well as the hydrophobic interaction between the A-ring of pelargonidin and Phe163, is crucial for GPR35 activation. Furthermore, pelargonidin inhibited the production of interleukin-8, a pro-inflammatory cytokine, by activating endogenous GPR35 in Caco-2 cells. These findings suggest that GPR35 may serve as a potential receptor for dietary anthocyanidins, such as pelargonidin, and provide new insights into the molecular mechanisms underlying the potential chemopreventive effects of anthocyanidins.

  6. Complex Chalcone Scaffolds from Mallotus philippinensis Fruits as Potential iNOS Inhibitors. International-journal Peer-reviewed

    Yen Thi Hoang Kieu, Hiroyuki Hattori, Masako Toda, Emiko Yanase

    Journal of natural products　2025/09/25

    DOI： 10.1021/acs.jnatprod.5c00739 　

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    The chalcone skeleton is a fundamental structural motif in numerous natural and synthetic compounds, playing a crucial role in various biological activities, including anticancer and anti-inflammatory effects. Mallotus philippinensis is a remarkable source of chalcones, recognized for its diverse chemical structures and significant bioactivity. The presence of complex dimeric chalcones in this species is both characteristic and rare. However, to the best of our knowledge, only five such complex dimeric chalcones have been identified to date. Here, we report six novel complex chalcones, runachalcones A-F (1-6), which significantly expand the chemical space of dimeric chalcones and highlight the untapped structural diversity within M. philippinensis fruits. Their planar structures were elucidated using a combination of NMR spectroscopy and HR-ESIMS, while their absolute configurations were determined through J-based configurational analysis, ROESY spectra, and quantum mechanical calculations of ECD. In an assay using a macrophage line RAW 264.7, only runachalcone F suppressed lipopolysaccharide (LPS)-induced expression of inducible nitric oxide synthase (iNOS) protein and the production of nitric oxide, suggesting that the compound possesses potential anti-inflammatory properties.

  7. Eriodictyol and thymonin act as GPR35 agonists International-journal Peer-reviewed

    Fumie Nakashima, Wei Qi Loh, Mayuka Wakabayashi, Sayako Shimomura, Hiroyuki Hattori, Masaki Kita, Asuka Inoue, Koji Uchida, Takahiro Shibata

    Bioscience, Biotechnology, and Biochemistry　2023/09

    DOI： 10.1093/bbb/zbad125 　

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    Although herbs and spices have been used in traditional medicine for more than a century owing to their health benefits, the associated underlying mechanism is still not clear. Since the G protein-coupled receptor 35 (GPR35) has been linked to exert various antioxidant and anti-inflammatory effects, we screened 19 different herbs and spices for possible GPR35 agonist(s) to understand the GPR35-dependent functions on herbs and spices. Among the screened extracts, the ethyl acetate extract of thyme exhibited a remarkable GPR35 agonistic activity. Activity-guided separations allowed us to identify two polyphenolic phytochemicals, eriodictyol and thymonin, acting as GPR35 agonists. Both eriodictyol and thymonin showed a potent and specific agonist activity toward GPR35 with half maximal effective concentration values of 5.48 and 8.41 μM, respectively. These findings indicate that these phytochemicals may have beneficial health effects upon GPR35 activation.

  8. Identification of Ecdysteroid Sinapate Esters with COX-2 Inhibitory Effects from Fibraurea recisa Using Molecular Networking and MS2LDA Peer-reviewed

    Yuya Kakumu, Minh Tu Thi Nguyen, Tohru Mitsunaga, Hiroyuki Hattori

    Journal of Natural Products　2023/08/02

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jnatprod.3c00371 　

    ISSN： 0163-3864

    eISSN： 1520-6025

  9. Advanced glycation end products and allergy Peer-reviewed

    Masako Toda, Michael Hellwig, Hiroyuki Hattori, Thomas Henle, Stefan Vieths

    Allergo Journal International　2023/06/28

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s40629-023-00259-4 　

    eISSN： 2197-0378

  10. Molecular networking-based lipid profiling and multi-omics approaches reveal new contributions of functional vanilloids to gut microbiota and lipometabolism changes. International-journal Peer-reviewed

    Hiroyuki Hattori, Akihiro Moriyama, Tomoki Ohno, Takahiro Shibata, Hitoshi Iwahashi, Tohru Mitsunaga

    Food chemistry. Molecular sciences　5　100123-100123　2022/12/30

    DOI： 10.1016/j.fochms.2022.100123 　

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    Obesity is now recognized as an epidemic, requiring rapid intervention. We previously demonstrated that vanilloids from the African spice known as Grains of Paradise (GOP) exhibit strong anti-obesity effects. Here, the molecular mechanism behind the obesity prevention property of the GOP extract was investigated by employing molecular networking-based lipid profiling and 16S rRNA sequencing. Administration of either GOP extract or two of its components, 6-paradol and 6-gingerol, reversed the unbalanced gut microbiota composition induced by a high-fat diet (HFD) with a decrease in Firmicutes / Bacteroidetes ratio and increase in genera Bifidobacterium and Akkermansia. Moreover, GOP extract ameliorated abnormal fatty acid metabolism in gut bacteria. Furthermore, lipid molecular networking revealed differences in lipid composition, such as triglycerides and bile acids, in the feces. These results suggest that 6-paradol and 6-gingerol can restore an unhealthy gut environment and prevent lipid absorption, a possible consequence of the reconstruction of the gut microbiota.

  11. 6‐Paradol acts as a potential anti‐obesity vanilloid from Grains of Paradise International-journal

    Hiroyuki Hattori, Takashi Mori, Takahiro Shibata, Masaki Kita, Tohru Mitsunaga

    Molecular Nutrition & Food Research　2100185-2100185　2021/04

    Publisher: Wiley

    DOI： 10.1002/mnfr.202100185 　

    ISSN： 1613-4125

    eISSN： 1613-4133

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    SCOPE: Grains of Paradise (GOP), the seeds of Aframomum melegueta, has anti-obesity effects. However, the mechanisms underlying the effects remain unclear. METHODS AND RESULTS: We set up to study the anti-obesity impact and homeostatic effects of 6-paradol, a major vanilloid found in GOP, and investigated the physiological outputs and the lipometabolism-related gene in fat and liver in high-fat-induced obese mice with a comparison with structurally similar vanilloids (6-gingerol and 6-shogaol). The vanilloids were synthesized in adequate quantities for performing animal experiments and orally administered to six-week-old male mice over two weeks. We found that 6-paradol decreased body weight gain and visceral and subcutaneous fats in two weeks, whereas 6-gingerol and 6-shogaol had no effect. Additionally, 6-paradol suppressed the hepatic cholesterol and triglyceride and significantly decreased the gene expression related to fatty acid synthesis, lipid transportation, and adipocyte differentiation in both liver and adipose tissue. Moreover, phosphorylation of AMP-activated protein kinase (AMPK) that greatly contributes to lipometabolism was promoted by 6-gingerol but not 6-paradol. CONCLUSION: These results suggest that 6-paradol regulates several obesity-related genes in an AMPK-independent manner. Therefore, it could be the principal active vanilloid in GOP giving it anti-obesity properties with a different mechanism. This article is protected by copyright. All rights reserved.

  12. Garcinoic Acids and a Benzophenone Derivative from the Seeds of Garcinia kola and Their Antibacterial Activities against Oral Bacterial Pathogenic Organisms Peer-reviewed

    Yusuke Hioki, Siaw Onwona-Agyeman, Yuya Kakumu, Hiroyuki Hattori, Kosei Yamauchi, Tohru Mitsunaga

    Journal of Natural Products　83　(7)　2087-2092　2020/07/24

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jnatprod.9b01045 　

    ISSN： 0163-3864

    eISSN： 1520-6025

  13. Synthesis of phenolic components of Grains of Paradise Peer-reviewed

    Hiroyuki Hattori, Tohru Mitsunaga, Derrick L. J. Clive

    TETRAHEDRON LETTERS　60　(30)　1989-1991　2019/07

    DOI： 10.1016/j.tetlet.2019.06.050 　

    ISSN： 0040-4039

  14. Identification of vanilloid compounds in grains of paradise and their effects on sympathetic nerve activity Peer-reviewed

    Hiroyuki Hattori, Kosei Yamauchi, Siaw Onwona-Agyeman, Tohru Mitsunaga

    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE　98　(12)　4742-4748　2018/09

    DOI： 10.1002/jsfa.9009 　

    ISSN： 0022-5142

    eISSN： 1097-0010

  15. Melanogenesis inhibitory activity of components from Salam leaf (Syzygium polyanthum) extract Peer-reviewed

    Andriyana Setyawati, Kae Hirabayashi, Kosei Yamauchi, Hiroyuki Hattori, Tohru Mitsunaga, Irmanida Batubara, Rudi Heryanto, Hiroshi Hashimoto, Mitsuyuki Hotta

    Journal of Natural Medicines　72　(2)　474-480　2018/03

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1007/s11418-018-1171-4 　

    ISSN： 1340-3443

    eISSN： 1861-0293

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    In order to identify a novel whitening agent, the methanol extract of S. polyanthum leaf was focused on by the screening test using nine Indonesian medicinal plants for the inhibition of melanogenesis and tyrosinase activity in B16 melanoma cells. Three novel compounds [(1) 1-(2,3,5-trihydroxy-4-methylphenyl)hexane-1-one, (2) 1-(2,3,5-trihydroxy methylphenyl)octane-1-one, and (3) (4E)-1-(2,3,5-trihydroxy-4-methylphenyl)decan-1-one and one known compound [(4) 1-(2,3,5-trihydroxy-4-methylphenyl)decan-1-one were isolated from the methanol extract. Our study demonstrated that S. polyanthum leaf methanol extract at 25-200 μg/mL decreased extracellular melanin formation ca. 20-80%, with high cell viability. Compounds 1-4 were found to be active in melanogenesis and tyrosinase inhibition. Compound 3 was the most active against tyrosinase activity (83.98 μM), particularly when L-tyrosine was the substrate. Compounds 1-4 significantly diminished extracellular melanin formation in B16 melanoma cells (> 80%), with high cell viability. Thus, our study suggested that compounds 1-4 isolated from the methanol extract of S. polyanthum leaf play important roles in decreasing extracellular melanogenesis and inhibiting tyrosinase.

  16. 香辛料Grains of Paradise成分の肥満抑制および交感神経活動に与える効果

    光永 徹, 服部 浩之

    AROMA RESEARCH　71　(18)　29-34　2017/08

  17. Effect of Grains of Paradise (GP) Extract Intake on Obesity and Sympathetic Nerve Activity Peer-reviewed

    Hiroyuki Hattori, Kosei Yamauchi, Siaw Onwona-Agyeman, Tohru Mitsunaga

    American Journal of Plant Sciences　08　(02)　85-95　2017/01

    Publisher: Scientific Research Publishing, Inc.

    DOI： 10.4236/ajps.2017.82007 　

    ISSN： 2158-2742

    eISSN： 2158-2750

  18. In vivo anti-obese effect of grains of paradise extract Peer-reviewed

    Hiroyuki Hattori, Tohru Mitsunaga, Siaw Onwona Agyeman

    CHEMICAL SENSES　41　(9)　E181-E181　2016/11

    ISSN： 0379-864X

    eISSN： 1464-3553

  19. Anti-obese effect of Grains of Paradise (GP) extract Peer-reviewed

    H. Hattori, S. Onwona-Agyeman, T. Mitsunaga

    Proceedings of the 5th UGSAS-GU & BWEL Joint International Symposium on Agricultural and Environmental Sciences　88-89　2016/08

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Misc. 8

  1. Dioscorea rotundataに含まれる機能性成分の探索

    黒木茜, 中島史恵, 柴田貴広, 服部浩之

    日本生薬学会年会講演要旨集　69th　2023

    ISSN： 0919-1992

  2. Analysis of post-translational modifications of proteins formed in a dyslipidemia-dependent manner

    西川七海, 中島史恵, 服部浩之, 内田浩二, 柴田貴広

    日本農芸化学会大会講演要旨集(Web)　2023　2023

    ISSN： 2186-7976

  3. Comprehensive analysis of lysine adducts formed in oxidized lipoproteins

    京屋陽介, 大塚優人, 服部浩之, 服部浩之, 中島史恵, 内田浩二, 柴田貴広

    日本農芸化学会大会講演要旨集(Web)　2023　2023

    ISSN： 2186-7976

  4. Anti-obesity of Dioscorea rotundata and the search for its bioactive constituents

    黒木茜, 服部浩之, 中島史恵, 柴田貴広

    日本農芸化学会大会講演要旨集(Web)　2023　2023

    ISSN： 2186-7976

  5. 香辛料Grains of Paradise成分の肥満抑制効果とその分子メカニズムの網羅的解明

    服部浩之, 山内恒生, 光永徹

    日本木材学会大会研究発表要旨集(完全版)(CD-ROM)　70th　2020

    ISSN： 1349-0532

  6. Grains of Paradise種子抽出成分の配糖化とスコポラミン誘導性記憶障害に対する効果

    山口佳穂, 服部浩之, 山内恒生, 光永徹, アジマンスィアウ

    日本木材学会大会研究発表要旨集(完全版)(CD-ROM)　69th　2019

    ISSN： 1349-0532

  7. Grains of Paradise種子抽出成分の配糖化とスコポラミン誘導性記憶障害に対する効果

    山口 佳穂, 晋山 典恵, 山内 恒生, 服部 浩之, 光永 徹

    日本生薬学会年会講演要旨集　65回　91-91　2018/08

    Publisher: (一社)日本生薬学会

    ISSN： 0919-1992

  8. 香辛料Grains of Paradise成分のラット交感神経活動に与える効果

    服部 浩之, 山内 恒生, 光永 徹

    日本生薬学会年会講演要旨集　64回　117-117　2017/08

    Publisher: (一社)日本生薬学会

    ISSN： 0919-1992

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Presentations 10

  1. Photoaffinity labelling strategy reveals a new anti-obesity target of capsaicin-like compound Invited

    2025/12/02

  2. 抗肥満作用に寄与する辛味香辛料成分の標的タンパク質探索

    服部浩之, 柴田貴広, 戸田雅子

    日本木材学会北海道・東北支部 令和7年度 (第57回) 研究発表会　2025/11/11

  3. ヨーロッパイエコオロギ抽出物の抗炎症作用と機能性成分の解析

    轟直也, 服部浩之, 戸田雅子

    日本栄養食糧学会東北支部第59回大会　2025/10/26

  4. 香辛料Grains of Paradise成分の抗肥満作用機序の解明

    服部浩之, 柴田貴広, 戸田雅子

    日本農芸化学会2025年度大会　2025/03/07

  5. 西アフリカ香辛料成分による肥満抑制と腸内環境改善効果の関係

    服部浩之, 柴田貴広, 光永徹

    第74回日本木材学会大会　2024/03/13

  6. Elucidation of the chemical structures and anti-oxidant property of Cambodian Melaleuca extract

    Ravor SENG,Akane KUROKI,Masashi KAWAGUCHI,Fumie NAKASHIMA,Takahiro SHIBATA,Hiroyuki HATTORI

    2024/03/09

  7. Dioscorea rotundataに含まれる機能性成分の探索

    黒木茜, 中島史恵, 柴田貴広, 服部浩之

    第69回日本生薬学会　2023/09/10

  8. 脂質分子ネットワーキングとマルチオミクス解析による西アフリカ香辛料成分の肥満抑制機構解明

    服部浩之, 柴田貴広, 光永 徹

    第69回日本生薬学会　2023/09/09

  9. Isolation and identification of a compound from Melaleuca extract and its bioactivities

    Ravor Seng, Akane Kuroki, Fumie Nakashima, Takahiro Shibata, Hiroyuki Hattori

    The 14th ICERD　2023/03/04

  10. 分子ネットワークより見出した新規エクジステロイド-シナピン酸複合体の構造と生物活性

    各務裕也, Minh Tu Thi Nguyen, 柴田貴広, 光永徹, 服部浩之

    第66回香料・テルペンおよび精油化学に関する討論会　2022/11/05

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Research Projects 17

  1. 抗アレルギー戦略におけるポリアミンの活用基盤の構築

    戸田 雅子, 柴田 貴広, 高橋 恭子, 服部 浩之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2025/04/01 - 2028/03/31

  2. Project for Improving the Quality of Cambodian Aromatic Oils – Towards Establishing the One Village, One Product Initiative –

    Offer Organization: Japan International Cooperation Agency

    2026/02 - 2028/01

  3. 細胞壁改変による農産物内在成分のバイオアベイラビリティ制御の可能性

    今泉 鉄平, 中野 浩平, 田中 史彦, 田村 匡嗣, 服部 浩之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(開拓)

    Institution: 岐阜大学

    2024/06/28 - 2027/03/31

  4. 樹木系香辛料バニロイドが示す機能的多様性の分子理解

    服部 浩之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 若手研究

    Institution: 東北大学

    2024/04/01 - 2027/03/31

  5. Exploration of agricultural and rural development approaches in the SDGs era: Empirical research on the effectiveness of nutrition-oriented agriculture.

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Nagoya University

    2022/04/01 - 2027/03/31

  6. メラルーカ精油を用いた高機能エディブルコーティングの開発

    System: みちのくGAPファンド (STEP1：モノづくり・Deep-Tech枠)

    2026/04 - 2027/03

  7. 疾患状態を反映する代謝物アダクトームの解明

    柴田 貴広, 中島 史恵, 板倉 正典, 三城 恵美, 服部 浩之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 挑戦的研究(開拓)

    Institution: 名古屋大学

    2023/06 - 2027/03

  8. Discovering Cambodian Traditional-foods and Establishing a Model for Sustaining traditional-foods by Improving Safety

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    Institution: Nagoya University

    2021/10 - 2027/03

  9. 昆虫資源における低酸素－迅速乾燥技術の研究開発

    今泉鉄平,服部浩之

    Offer Organization: 国立研究開発法人新エネルギー・産業技術総合開発機構

    System: 官民による若手研究者発掘支援事業 共同研究フェーズ

    Institution: 東北大学

    2024/04 - 2026/03

  10. 光親和性標識法による辛味バニロイドの新たな標的受容体探索

    Offer Organization: 公益財団法人 東洋食品研究所

    System: 2023年度研究助成

    2024/04 - 2025/03

  11. 減圧マイクロ波乾燥によるコオロギ粉末の高品質化と有用成分の探索

    今泉鉄平, 服部浩之

    Offer Organization: 国立研究開発法人新エネルギー・産業技術総合開発機構

    System: 官民による若手研究者発掘支援事業 マッチングサポートフェーズ

    Institution: 名古屋大学

    2022/02 - 2024/03

  12. 樹木系香辛料成分の抗肥満活性：作用点の違いから理解するバニロイドの機能的多様性

    服部 浩之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 若手研究

    Category: 若手研究

    Institution: 名古屋大学

    2021/04 - 2024/03

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    本研究では，西アフリカ原産の香辛料Grains of Paradise (GOP) の示した肥満抑制作用の分子メカニズムを解明することを目的とした。これまでの検証から，GOPに含まれるバニロイド成分は，トウガラシの主要な辛味バニロイドであるカプサイシンとは異なり，交感神経系を介さない経路により肥満抑制作用を示すことを発見した。また，GOPバニロイドは非常に類似した構造を有するにも関わらず，脂肪蓄積や脂質代謝に対する効果が異なることを見出した。これらの結果は，GOPバニロイドがカプサイシンとは異なる標的タンパク質との相互作用を介して肥満抑制作用を発現することを示唆しており，肥満抑制効果や活性作用点に対する機能的多様性を有すると考えられる。これらの背景から，本研究では，GOPバニロイドの中でも特に側鎖構造のみがわずかに異なる6-paradolや6-gingerol，6-shogaolに着目し，以下の項目について調査した。 1) マウス3T3L1線維芽細胞 （前駆脂肪細胞） を常法で分化誘導した脂肪細胞にGOP抽出物やGOPバニロイドを投与し，Oil Red O染色法により検出したところ，GOP抽出物や6-shogaolの投与により脂肪蓄積が減少した一方で，6-paradolや6-gingerolの投与により脂肪蓄積が増加した。 2) GOPバニロイドの脂肪細胞分化に対する作用が異なることから，フォトアフィニティープローブ合成を行った。既報に従い，GOPバニロイドを調製し，光反応性官能基にはベンゾフェノンを用いた。パラジヒドロキシベンゾフェノンに対して，プロパギルブロマイドを用いて末端アルキンを導入した。また，ウィリアムソン合成により，アルキルリンカーを導入した後，バニロイドの有するフェノール性水酸基と結合することで，フォトアフィニティープローブを合成した。

  13. 脂質分子ネットワーキングとマルチオミクス解析による西アフリカ香辛料の肥満抑制機構解明に関する研究

    服部 浩之

    Offer Organization: 公益財団法人 山崎香辛料振興財団

    System: 令和4年度研究助成

    Institution: 名古屋大学

    2022/10 - 2023/09

  14. カンボジア産香料・食用植物の新奇機能開拓と活性成分の探索

    服部浩之, 伊藤香純, Seng Ravor, Kong Sothea

    Offer Organization: アジア共創教育研究機構 融合プロジェクト

    Institution: アジア共創教育研究機構 融合プロジェクト

    2022/10 - 2023/03

  15. 「抗肥満受容体」の活性化能を指標とした新規機能性食用植物成分の探索 Competitive

    服部 浩之

    Offer Organization: 公益財団法人 戸部眞紀財団

    System: 2021年度 (令和3年度) 研究助成

    2021/10 - 2022/09

  16. インドネシア産薬用植物・食品由来の新奇有用物質の発見とその応用利用に関する研究

    Offer Organization: アジア共創教育研究機構

    System: 融合プロジェクト

    2021/08 - 2022/03

  17. 樹木系香辛料成分の肥満改善効果とその分子メカニズムの網羅的研究

    服部 浩之

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特別研究員奨励費

    Category: 特別研究員奨励費

    Institution: 岐阜大学

    2019/04/25 - 2021/03/31

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    樹木系香辛料Grains of Paradise(GOP)から単離・構造決定した化合物のうち，新規化合物，絶対立体配置の決まっていないキラル化合物，および主要成分(6-gingerol, 6-shogaol, 6-paradol)の全合成を試みた。バニリンを出発物質としてWittig反応や水素化，DIBAL還元反応などを経て新規化合物(5E)-8-(4-hydroxy-3-methoxyphenyl)oct-5-en-4-one(1)の全合成に成功した。また，不斉中心を有する4-[(3-hydroxydecyl]-2-methoxyphenol(2)をリンゴ酸を出発物質にキラル合成し，S体およびそのラセミ体の全合成に成功した。S体，ラセミ体，および天然型の化合物(2)をキラルカラムHPLC分析に供し，天然型がR:S=1:1.7の割合であるキラル化合物であることを証明した。GOP主要成分(6-gingerol, 6-shogaol, 6-paradol)はバニリンを出発物質に大量合成し，各成分の肥満抑制効果を検証するために動物実験に供した。高脂肪食を給餌したマウスにGOP抽出物や合成した主要成分を経口投与しながら2週間飼育し，各脂質パラメーターを測定したところ，興味深いことに6-gingerolは肝臓中の脂質代謝に対して，6-paradolは脂肪蓄積に対して特に効果を示すことが明らかとなった。さらに，GOP抽出物や各主要成分の示した肥満抑制効果の具体的な作用機序を解明するために，肝臓や脂肪組織における肥満関連遺伝子発現について，RealTime-PCR解析を試みているところである。 ここまでの成果は，国内学会1件，国際論文１報として公表した。

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Teaching Experience 8

  1. Introductory Science Experiments　Tohoku University

  2. Advanced Lecture on Food Science　Tohoku University

  3. Experiments in Agricultural Chemistry Ⅰ　Tohoku University

  4. Reading of Scientific EnglishⅠ　Tohoku University

  5. Integrated Agricultural Seminar　Gifu University

  6. Food Analysis　Chubu University

  7. Research Methods in Applied Biosciences　Nagoya University

  8. The special lecture on Chemical Biology　Nagoya University

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