Details of the Researcher

PHOTO

Atsushi Higashitani
Section
Graduate School of Life Sciences
Job title
Professor
Degree

  • 理学博士（名古屋大学）

  • 農学修士（京都工芸繊維大学）

e-Rad No.
40212162

Research History 3

  • 2001/04 - Present
    東北大学 大学院生命科学研究科　教授

  • 1997/03 - 2001/03
    東北大学 遺伝生態研究センター 適応生態研究部門　助教授

  • 1990/03 - 1997/02
    国立遺伝学研究所 細胞遺伝研究系　助手 総合研究大学院大学 遺伝学専攻 助手（兼担） （1995-1997)

Education 3

  • Nagoya University　Graduate School, Division of Natural Science　生物学専攻

    - 1990/02/28

  • Kyoto Institute of Technology　Graduate School, Division of Textile Science and Technology　応用生物学専攻

    - 1987/03/31

  • Kyoto Institute of Technology　Faculty of Textile Science and Technology　蚕糸生物学科

    - 1985/03/31

Professional Memberships 2

  • 日本ミトコンドリア学会

  • 日本宇宙生物科学会

Research Interests 10

  • イネ低温障害（冷害）

  • モデル生物

  • 筋萎縮

  • ミトコンドリア

  • high temperature injury

  • radiation effect

  • meiosis

  • DNA repair

  • cell cycle

  • genome

Research Areas 4

  • Life sciences / Genetics /

  • Environmental science/Agricultural science / Environmental effects of chemicals /

  • Environmental science/Agricultural science / Environmental effects of radiation /

  • Life sciences / Molecular biology / Molecular Biology

Awards 6

  1. Catalyst Award

    2020/10　National Academy of Medicine　Exploring ways to maintain and improve elderly muscle function with mitochondrial mechano-medicine

  2. 日本宇宙生物科学会賞

    2013/09/27　日本宇宙生物科学会

  3. 学術奨励賞

    2007/12/09　日本植物学会東北支部大会　オオムギとシロイヌナズナを用いた花粉形成時にみられる高温障害の研究

  4. Genes and Genetic Systems GSS Prize 2005

    2005/09　日本遺伝学会

  5. 山田科学振興財団 研究援助

    2001/07　財団法人 山田科学振興財団　減数分裂過程の染色体ダイナミクスとその制御機構に関する研究

  6. 日産学術研究助成

    1998/02　日産科学振興財団　細胞周期checkpoint制御の研究

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Papers 157

  1. Mitochonic acid 5 attenuates age-related neuromuscular dysfunction associated with mitochondrial Ca2+ overload in Caenorhabditis elegans. International-journal

    XinTong Wu, Miku Seida, Takaaki Abe, Atsushi Higashitani

    npj aging　9　(1)　20-20　2023/08/01

    DOI： 10.1038/s41514-023-00116-2 　

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    Mitochonic acid-5 ameliorates the pathophysiology of human mitochondrial-disease fibroblasts and Caenorhabditis elegans Duchenne muscular dystrophy and Parkinson's disease models. Here, we found that 10 μM MA-5 attenuates the age-related decline in motor performance, loss of muscle mitochondria, and degeneration of dopaminergic neurons associated with mitochondrial Ca2+ overload in C. elegans. These findings suggest that MA-5 may act as an anti-aging agent against a wide range of neuromuscular dysfunctions in metazoans.

  2. Increased mitochondrial Ca 2+ contributes to health decline with age and Duchene muscular dystrophy in C. elegans International-journal

    Atsushi Higashitani, Mika Teranishi, Yui Nakagawa, Yukou Itoh, Surabhi Sudevan, Nathaniel J. Szewczyk, Yukihiko Kubota, Takaaki Abe, Takeshi Kobayashi

    The FASEB Journal　37　(4)　e22851　2023/03/19

    Publisher: Wiley

    DOI： 10.1096/fj.202201489rr 　

    ISSN： 0892-6638

    eISSN： 1530-6860

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    Sarcopenia is a geriatric syndrome characterized by an age-related decline in skeletal muscle mass and strength. Here, we show that suppression of mitochondrial calcium uniporter (MCU)-mediated Ca2+ influx into mitochondria in the body wall muscles of the nematode Caenorhabditis elegans improved the sarcopenic phenotypes, blunting movement and mitochondrial structural and functional decline with age. We found that normally aged muscle cells exhibited elevated resting mitochondrial Ca2+ levels and increased mitophagy to eliminate damaged mitochondria. Similar to aging muscle, we found that suppressing MCU function in muscular dystrophy improved movement via reducing elevated resting mitochondrial Ca2+ levels. Taken together, our results reveal that elevated resting mitochondrial Ca2+ levels contribute to muscle decline with age and muscular dystrophy. Further, modulation of MCU activity may act as a potential pharmacological target in various conditions involving muscle loss.

  3. Loss of physical contact in space alters the dopamine system in C. elegans. International-journal

    Surabhi Sudevan, Kasumi Muto, Nahoko Higashitani, Toko Hashizume, Akira Higashibata, Rebecca A Ellwood, Colleen S Deane, Mizanur Rahman, Siva A Vanapalli, Timothy Etheridge, Nathaniel J Szewczyk, Atsushi Higashitani

    iScience　25　(2)　103762-103762　2022/02/18

    DOI： 10.1016/j.isci.2022.103762 　

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    Progressive neuromuscular decline in microgravity is a prominent health concern preventing interplanetary human habitation. We establish functional dopamine-mediated impairments as a consistent feature across multiple spaceflight exposures and during simulated microgravity in C. elegans. Animals grown continuously in these conditions display reduced movement and body length. Loss of mechanical contact stimuli in microgravity elicits decreased endogenous dopamine and comt-4 (catechol-O-methyl transferase) expression levels. The application of exogenous dopamine reverses the movement and body length defects caused by simulated microgravity. In addition, increased physical contact made comt-4 and dopamine levels rise. It also increased muscular cytoplasmic Ca2+ firing. In dop-3 (D2-like receptor) mutants, neither decrease in movement nor in body length were observed during simulated microgravity growth. These results strongly suggest that targeting the dopamine system through manipulation of the external environment (contact stimuli) prevents muscular changes and is a realistic and viable treatment strategy to promote safe human deep-space travel.

  4. Mitochondrial dysfunction causes Ca2+ overload and ECM degradation-mediated muscle damage in C. elegans. International-journal Peer-reviewed

    Surabhi Sudevan, Mai Takiura, Yukihiko Kubota, Nahoko Higashitani, Michael Cooke, Rebecca A Ellwood, Timothy Etheridge, Nathaniel J Szewczyk, Atsushi Higashitani

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology　33　(8)　9540-9550　2019/08

    DOI： 10.1096/fj.201802298R 　

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    Mitochondrial dysfunction impairs muscle health and causes subsequent muscle wasting. This study explores the role of mitochondrial dysfunction as an intramuscular signal for the extracellular matrix (ECM)-based proteolysis and, consequentially, muscle cell dystrophy. We found that inhibition of the mitochondrial electron transport chain causes paralysis as well as muscle structural damage in the nematode Caenorhabditis elegans. This was associated with a significant decline in collagen content. Both paralysis and muscle damage could be rescued with collagen IV overexpression, matrix metalloproteinase (MMP), and Furin inhibitors in Antimycin A-treated animal as well as in the C. elegans Duchenne muscular dystrophy model. Additionally, muscle cytosolic calcium increased in the Antimycin A-treated worms, and its down-regulation rescued the muscle damage, suggesting that calcium overload acts as one of the early triggers and activates Furin and MMPs for collagen degradation. In conclusion, we have established ECM degradation as an important pathway of muscle damage.-Sudevan, S., Takiura, M., Kubota, Y., Higashitani, N., Cooke, M., Ellwood, R. A., Etheridge, T., Szewczyk, N. J., Higashitani, A. Mitochondrial dysfunction causes Ca2+ overload and ECM degradation-mediated muscle damage in C. elegans.

  5. Reduction of Gibberellin by Low Temperature Disrupts Pollen Development in Rice1[W][OPEN] International-journal Peer-reviewed

    Tadashi Sakata, Susumu Oda, Yuta Tsunaga, Hikaru Shomura, Makiko Kawagishi-Kobayashi, Koichiro Aya, Kenichi Saeki, Takashi Endo, Kuniaki Nagano, Mikiko Kojima, Hitoshi Sakakibara, Masao Watanabe, Makoto Matsuoka, Atsushi Higashitani

    PLANT PHYSIOLOGY　164　(4)　2011-2019　2014/04

    DOI： 10.1104/pp.113.234401 　

    ISSN： 0032-0889

    eISSN： 1532-2548

  6. Auxins reverse plant male sterility caused by high temperatures Peer-reviewed

    Tadashi Sakata, Takeshi Oshino, Shinya Miura, Mari Tomabechi, Yuta Tsunaga, Nahoko Higashitani, Yutaka Miyazawa, Hideyuki Takahashi, Masao Watanabe, Atsushi Higashitani

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　107　(19)　8569-8574　2010/05

    DOI： 10.1073/pnas.1000869107 　

    ISSN： 0027-8424

  7. Minus-strand origin of filamentous phage versus transcriptional promoters in recognition of RNA polymerase Peer-reviewed

    A Higashitani, N Higashitani, K Horiuchi

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　94　(7)　2909-2914　1997/04

    DOI： 10.1073/pnas.94.7.2909 　

    ISSN： 0027-8424

  8. The Growth of Soybean (Glycine max) Under Salt Stress Is Modulated in Simulated Microgravity Conditions

    Setsuko Komatsu, Haruka Misaki, Wei Zhu, Hisateru YAMAGUCHI, Keisuke Hitachi, Kunihiro Tsuchida, Atsushi Higashitani

    Cells　2025/04

    DOI： 10.3390/cells14070541 　

  9. Gene expression and mucilage adaptations to salinity in germination of extreme halophyte Schrenkiella parvula seeds International-journal

    Keriman Şekerci, Nahoko Higashitani, Rengin Ozgur, Atsushi Higashitani, Ismail Turkan, Baris Uzilday

    Plant Physiology and Biochemistry　220　109517-109517　2025/03

    DOI： 10.1016/j.plaphy.2025.109517 　

    ISSN： 0981-9428

    eISSN： 1873-2690

  10. Induction of systemic resistance through calcium signaling in Arabidopsis exposed to air plasma-generated dinitrogen pentoxide International-journal

    Shota Sasaki, Hiroto Iwamoto, Keisuke Takashima, Masatsugu Toyota, Atsushi Higashitani, Toshiro Kaneko

    PLOS ONE　20　(2)　e0318757　2025/02/06

    DOI： 10.1371/journal.pone.0318757 　

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    Plasma technology, which can instantaneously transform air molecules into reactive species stimulating plants, potentially contributes to developing a sustainable agricultural system with high productivity and low environmental impact. In fact, plant immunity activation by exposure to a reactive gas mainly consisting of dinitrogen pentoxide (N2O5) was recently discovered, while physiological responses to N2O5 are rarely known. Here, we demonstrate early (within 10 min) physiological responses to N2O5 gas in Arabidopsis. Exposure to N2O5 gas induced an increase in cytosolic Ca2+ concentration within seconds in directly exposed leaves, followed by systemic long-distance Ca2+-based signaling within tens of seconds. In addition, jasmonic acid (JA)-related gene expression was induced within 10 minutes, and a significant upregulation of the defense-related gene PDF1.2 was observed after 1 day of exposure to N2O5 gas. These systemic resistant responses to N2O5 were found unique among air-plasma-generated species such as ozone (O3) and nitric oxide (NO)/nitrogen dioxide (NO2). Our results provide new insights into understanding of plant physiological responses to air-derived reactive species, in addition to facilitating the development of plasma applications in agriculture.

  11. Plasma nitrogen fixation for plant cultivation with air‐derived dinitrogen pentoxide

    Shouki Takeshi, Keisuke Takashima, Shota Sasaki, Atsushi Higashitani, Toshiro Kaneko

    Plasma Processes and Polymers　21　(10)　2024/07/11

    DOI： 10.1002/ppap.202400096 　

    ISSN： 1612-8850

    eISSN： 1612-8869

  12. Increase of secondary metabolites in sweet basil (Ocimum basilicum L.) leaves by exposure to N2O5 with plasma technology. International-journal

    Rie Tateishi, Natsumi Ogawa-Kishida, Nobuharu Fujii, Yuji Nagata, Yoshiyuki Ohtsubo, Shota Sasaki, Keisuke Takashima, Toshiro Kaneko, Atsushi Higashitani

    Scientific reports　14　(1)　12759-12759　2024/06/04

    DOI： 10.1038/s41598-024-63508-8 　

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    Exposure to N2O5 generated by plasma technology activates immunity in Arabidopsis through tryptophan metabolites. However, little is known about the effects of N2O5 exposure on other plant species. Sweet basil synthesizes many valuable secondary metabolites in its leaves. Therefore, metabolomic analyses were performed at three different exposure levels [9.7 (Ex1), 19.4 (Ex2) and 29.1 (Ex3) μmol] to assess the effects of N2O5 on basil leaves. As a result, cinnamaldehyde and phenolic acids increased with increasing doses. Certain flavonoids, columbianetin, and caryophyllene oxide increased with lower Ex1 exposure, cineole and methyl eugenol increased with moderate Ex2 exposure and L-glutathione GSH also increased with higher Ex3 exposure. Furthermore, gene expression analysis by quantitative RT-PCR showed that certain genes involved in the syntheses of secondary metabolites and jasmonic acid were significantly up-regulated early after N2O5 exposure. These results suggest that N2O5 exposure increases several valuable secondary metabolites in sweet basil leaves via plant defense responses in a controllable system.

  13. Solo regulates the localization and activity of PDZ-RhoGEF for actin cytoskeletal remodeling in response to substrate stiffness. International-journal

    Aoi Kunitomi, Shuhei Chiba, Nahoko Higashitani, Atsushi Higashitani, Shinichi Sato, Kensaku Mizuno, Kazumasa Ohashi

    Molecular biology of the cell　35　(6)　mbcE23110421　2024/04/24

    DOI： 10.1091/mbc.E23-11-0421 　

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    Recent findings indicate that Solo, a RhoGEF, is involved in cellular mechanical stress responses. However, the mechanism of actin cytoskeletal remodeling via Solo remains unclear. Therefore, this study aimed to identify Solo-interacting proteins using the BioID, a proximal-dependent labeling method, and elucidate the molecular mechanisms of function of Solo. We identified PDZ-RhoGEF (PRG) as a Solo-interacting protein. PRG co-localized with Solo in the basal area of cells, depending on Solo localization, and enhanced actin polymerization at the Solo accumulation sites. Additionally, Solo and PRG interaction was necessary for actin cytoskeletal remodeling. Furthermore, the purified Solo itself had little or negligible GEF activity, even the GEF-inactive mutant directly activated the GEF activity of the PRG through interaction. Moreover, overexpression of the Solo and PRG binding domains, respectively, had a dominant-negative effect on actin polymerization and actin stress fiber formation in response to substrate stiffness. Therefore, Solo restricts the localization of PRG and regulates actin cytoskeletal remodeling in synergy with PRG in response to the surrounding mechanical environment.

  14. Proteomic profiling of an extreme halophyte Schrenkiella parvula with accelerated root elongation under mild salt stress.

    Keriman Şekerci, Nahoko Higashitani, Rengin Ozgur, Baris Uzilday, Atsushi Higashitani, Ismail Turkan

    Plant Stress　11　2024/03

    DOI： 10.1016/j.stress.2024.100357 　

    ISSN： 2667-064X

  15. SoloとPDZ-RhoGEFの相互作用を介したメカノストレス応答におけるアクチン骨格の制御機構

    國富 葵, 千葉 秀平, 東谷 篤志, 東谷 なほ子, 水野 健作, 大橋 一正

    日本生化学会大会プログラム・講演要旨集　96回　[1P-251]　2023/10

    Publisher: (公社)日本生化学会

  16. Comparative Analysis of Muscle Atrophy During Spaceflight, Nutritional Deficiency and Disuse in the Nematode Caenorhabditis elegans. International-journal

    Ban-Seok Kim, Alfredo V Alcantara Jr, Je-Hyun Moon, Atsushi Higashitani, Nahoko Higashitani, Timothy Etheridge, Nathaniel J Szewczyk, Colleen S Deane, Christopher J Gaffney, Akira Higashibata, Toko Hashizume, Kyoung-Hye Yoon, Jin I Lee

    International journal of molecular sciences　24　(16)　2023/08/10

    DOI： 10.3390/ijms241612640 　

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    While spaceflight is becoming more common than before, the hazards spaceflight and space microgravity pose to the human body remain relatively unexplored. Astronauts experience muscle atrophy after spaceflight, but the exact reasons for this and solutions are unknown. Here, we take advantage of the nematode C. elegans to understand the effects of space microgravity on worm body wall muscle. We found that space microgravity induces muscle atrophy in C. elegans from two independent spaceflight missions. As a comparison to spaceflight-induced muscle atrophy, we assessed the effects of acute nutritional deprivation and muscle disuse on C. elegans muscle cells. We found that these two factors also induce muscle atrophy in the nematode. Finally, we identified clp-4, which encodes a calpain protease that promotes muscle atrophy. Mutants of clp-4 suppress starvation-induced muscle atrophy. Such comparative analyses of different factors causing muscle atrophy in C. elegans could provide a way to identify novel genetic factors regulating space microgravity-induced muscle atrophy.

  17. Caenorhabditis elegans in microgravity: An omics perspective. International-journal

    Amanda Scott, Craig R G Willis, Masafumi Muratani, Atsushi Higashitani, Timothy Etheridge, Nathaniel J Szewczyk, Colleen S Deane

    iScience　26　(7)　107189-107189　2023/07/21

    DOI： 10.1016/j.isci.2023.107189 　

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    The application of omics to study Caenorhabditis elegans (C. elegans) in the context of spaceflight is increasing, illuminating the wide-ranging biological impacts of spaceflight on physiology. In this review, we highlight the application of omics, including transcriptomics, genomics, proteomics, multi-omics, and integrated omics in the study of spaceflown C. elegans, and discuss the impact, use, and future direction of this branch of research. We highlight the variety of molecular alterations that occur in response to spaceflight, most notably changes in metabolic and neuromuscular gene regulation. These transcriptional features are reproducible and evident across many spaceflown species (e.g., mice and astronauts), supporting the use of C. elegans as a model organism to study spaceflight physiology with translational capital. Integrating tissue-specific, spatial, and multi-omics approaches, which quantitatively link molecular responses to phenotypic adaptations, will facilitate the identification of candidate regulatory molecules for therapeutic intervention and thus represents the next frontiers in C. elegans space omics research.

  18. Self-grafting induced epigenetic changes leading to drought stress tolerance in tomato plants International-journal

    Maria Isabel Fuentes-Merlos, Masaru Bamba, Shusei Sato, Atsushi Higashitani

    DNA Research　30　(4)　2023/07/15

    Publisher: Oxford University Press (OUP)

    DOI： 10.1093/dnares/dsad016 　

    ISSN： 1340-2838

    eISSN： 1756-1663

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    Abstract Grafting is widely used as a method to increase stress tolerance in good fruiting lines of Solanaceae plants. However, little is known about how grafting, affects epigenetic modifications and leads to stress tolerance, especially within the same line. Here, we studied the effects of self-grafting in tomato plants on histone and DNA modifications and changes in gene expression related to drought stress. We found that at the 3-leaf stage, one week after self-grafting, histone H3 K4 trimethylation and K27 trimethylation changes were observed in more than 500 genes each, and DNA methylation changes in more than 5,000 gene regions at the shoot apex compared to the non-grafted control. In addition, two weeks after the epigenomic changes, global expression changes continued to be observed at the shoot apex in several genes related to the metabolic process of nitrogen compounds, responses to stimulus, chromosome organization, cell cycle-related genes, and regulation of hormone levels. Finally, these grafted seedlings acquired remarkable drought tolerance, suggesting that epigenomic modifications during the wound-healing process mitigate stress tolerance in tomato plants.

  19. Proteomic features of skeletal muscle adaptation to resistance exercise training as a function of age. International-journal

    Colleen S Deane, Bethan E Phillips, Craig R G Willis, Daniel J Wilkinson, Ken Smith, Nahoko Higashitani, John P Williams, Nathaniel J Szewczyk, Philip J Atherton, Atsushi Higashitani, Timothy Etheridge

    GeroScience　45　(3)　1271-1287　2023/06

    DOI： 10.1007/s11357-022-00658-5 　

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    Resistance exercise training (RET) can counteract negative features of muscle ageing but older age associates with reduced adaptive capacity to RET. Altered muscle protein networks likely contribute to ageing RET adaptation; therefore, associated proteome-wide responses warrant exploration. We employed quantitative sarcoplasmic proteomics to compare age-related proteome and phosphoproteome responses to RET. Thigh muscle biopsies were collected from eight young (25 ± 1.1 years) and eight older (67.5 ± 2.6 years) adults before and after 20 weeks supervised RET. Muscle sarcoplasmic fractions were pooled for each condition and analysed using Isobaric Tags for Relative and Absolute Quantification (iTRAQ) labelling, tandem mass spectrometry and network-based hub protein identification. Older adults displayed impaired RET-induced adaptations in whole-body lean mass, body fat percentage and thigh lean mass (P > 0.05). iTRAQ identified 73 differentially expressed proteins with age and/or RET. Despite possible proteomic stochasticity, RET improved ageing profiles for mitochondrial function and glucose metabolism (top hub; PYK (pyruvate kinase)) but failed to correct altered ageing expression of cytoskeletal proteins (top hub; YWHAZ (14-3-3 protein zeta/delta)). These ageing RET proteomic profiles were generally unchanged or oppositely regulated post-RET in younger muscle. Similarly, RET corrected expression of 10 phosphoproteins altered in ageing, but these responses were again different vs. younger adults. Older muscle is characterised by RET-induced metabolic protein profiles that, whilst not present in younger muscle, improve untrained age-related proteomic deficits. Combined with impaired cytoskeletal adhesion responses, these results provide a proteomic framework for understanding and optimising ageing muscle RET adaptation.

  20. A stratagem for primary root elongation under moderate salt stress in the halophyte Schrenkiella parvula International-journal

    Keriman Şekerci, Nahoko Higashitani, Rengin Ozgur, Baris Uzilday, Atsushi Higashitani, Ismail Turkan

    Physiologia Plantarum　175　(3)　e13937　2023/05

    DOI： 10.1111/ppl.13937 　

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    Schrenkiella parvula, an Arabidopsis-related halophyte, grows around Lake Tuz (Salt) in Turkey and can survive up to 600 mM NaCl. Here, we performed physiological studies on the roots of S. parvula and A. thaliana seedlings cultivated under a moderate salt condition (100 mM NaCl). Interestingly, S. parvula germinated and grew at 100 mM NaCl, but germination did not occur at salt concentrations above 200 mM. In addition, primary roots elongated much faster at 100 mM NaCl, while being thinner with fewer roots hair, than under NaCl-free conditions. Salt-induced root elongation was due to epidermal cell elongation, but meristem size and meristematic DNA replication were reduced. The expression of genes related to auxin response and biosynthesis was also reduced. Application of exogenous auxin abolished the changes in primary root elongation, suggesting that auxin reduction is the main trigger for root architectural changes in response to moderate salinity in S. parvula. In A. thaliana seeds, germination was maintained up to 200 mM NaCl, but post-germination root elongation was significantly inhibited. Furthermore, primary roots did not promote elongation even under fairly low salt conditions. Compared to A. thaliana, cell death and ROS content in primary roots of salt-stressed plants were significantly lower in S. parvula. These changes in the roots of S. parvula seedlings may be an adaptive strategy to reach lower salinity by advancing into deeper soils, while being impaired by moderate salt stress.

  21. Cover Image

    Atsushi Higashitani, Mika Teranishi, Yui Nakagawa, Yukou Itoh, Surabhi Sudevan, Nathaniel J. Szewczyk, Yukihiko Kubota, Takaaki Abe, Takeshi Kobayashi

    The FASEB Journal　2023/04

    DOI： 10.1096/fsb2.22909 　

  22. 222 nm far-UVC efficiently introduces nerve damage in Caenorhabditis elegans. International-journal

    Kaoru Okamoto Yoshiyama, Norihiko L Okamoto, Jun Hidema, Atsushi Higashitani

    PloS one　18　(1)　e0281162　2023

    DOI： 10.1371/journal.pone.0281162 　

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    Far-ultraviolet radiation C light (far-UVC; 222 nm wavelength) has received attention as a safer light for killing pathogenic bacteria and viruses, as no or little DNA damage is observed after irradiation in mammalian skin models. Far-UVC does not penetrate deeply into tissues; therefore, it cannot reach the underlying critical basal cells. However, it was unclear whether far-UVC (222-UVC) irradiation could cause more biological damage at shallower depths than the 254 nm UVC irradiation (254-UVC), which penetrates more deeply. This study investigated the biological effects of 222- and 254-UVC on the small and transparent model organism Caenorhabditis elegans. At the same energy level of irradiation, 222-UVC introduced slightly less cyclobutane pyrimidine dimer damage to naked DNA in solution than 254-UVC. The survival of eggs laid during 0-4 h after irradiation showed a marked decrease with 254-UVC but not 222-UVC. In addition, defect of chromosomal condensation was observed in a full-grown oocyte by 254-UVC irradiation. In contrast, 222-UVC had a significant effect on the loss of motility of C. elegans. The sensory nervous system, which includes dopamine CEP and PVD neurons on the body surface, was severely damaged by 222-UVC, but not by the same dose of 254-UVC. Interestingly, increasing 254-UVC irradiation by about 10-fold causes similar damage to CEP neurons. These results suggest that 222-UVC is less penetrating, so energy transfer occurs more effectively in tissues near the surface, causing more severe damage than 254-UVC.

  23. Global landscape of replicative DNA polymerase usage in the human genome. International-journal

    Eri Koyanagi, Yoko Kakimoto, Tamiko Minamisawa, Fumiya Yoshifuji, Toyoaki Natsume, Atsushi Higashitani, Tomoo Ogi, Antony M Carr, Masato T Kanemaki, Yasukazu Daigaku

    Nature communications　13　(1)　7221-7221　2022/11/24

    DOI： 10.1038/s41467-022-34929-8 　

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    The division of labour among DNA polymerase underlies the accuracy and efficiency of replication. However, the roles of replicative polymerases have not been directly established in human cells. We developed polymerase usage sequencing (Pu-seq) in HCT116 cells and mapped Polε and Polα usage genome wide. The polymerase usage profiles show Polε synthesises the leading strand and Polα contributes mainly to lagging strand synthesis. Combining the Polε and Polα profiles, we accurately predict the genome-wide pattern of fork directionality plus zones of replication initiation and termination. We confirm that transcriptional activity contributes to the pattern of initiation and termination and, by separately analysing the effect of transcription on co-directional and converging forks, demonstrate that coupled DNA synthesis of leading and lagging strands is compromised by transcription in both co-directional and convergent forks. Polymerase uncoupling is particularly evident in the vicinity of large genes, including the two most unstable common fragile sites, FRA3B and FRA3D, thus linking transcription-induced polymerase uncoupling to chromosomal instability. Together, our result demonstrated that Pu-seq in human cells provides a powerful and straightforward methodology to explore DNA polymerase usage and replication fork dynamics.

  24. Mitochonic Acid 5 Improves Duchenne Muscular Dystrophy and Parkinson's Disease Model of Caenorhabditis elegans. International-journal

    Xintong Wu, Satoi Nagasawa, Kasumi Muto, Maiko Ueda, Chitose Suzuki, Takaaki Abe, Atsushi Higashitani

    International journal of molecular sciences　23　(17)　2022/08/24

    DOI： 10.3390/ijms23179572 　

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    Mitochonic Acid 5 (MA-5) enhances mitochondrial ATP production, restores fibroblasts from mitochondrial disease patients and extends the lifespan of the disease model "Mitomouse". Additionally, MA-5 interacts with mitofilin and modulates the mitochondrial inner membrane organizing system (MINOS) in mammalian cultured cells. Here, we used the nematode Caenorhabditis elegans to investigate whether MA-5 improves the Duchenne muscular dystrophy (DMD) model. Firstly, we confirmed the efficient penetration of MA-5 in the mitochondria of C. elegans. MA-5 also alleviated symptoms such as movement decline, muscular tone, mitochondrial fragmentation and Ca2+ accumulation of the DMD model. To assess the effect of MA-5 on mitochondria perturbation, we employed a low concentration of rotenone with or without MA-5. MA-5 significantly suppressed rotenone-induced mitochondria reactive oxygen species (ROS) increase, mitochondrial network fragmentation and nuclear destruction in body wall muscles as well as endogenous ATP levels decline. In addition, MA-5 suppressed rotenone-induced degeneration of dopaminergic cephalic (CEP) neurons seen in the Parkinson's disease (PD) model. Furthermore, the application of MA-5 reduced mitochondrial swelling due to the immt-1 null mutation. These results indicate that MA-5 has broad mitochondrial homing and MINOS stabilizing activity in metazoans and may be a therapeutic agent for these by ameliorating mitochondrial dysfunction in DMD and PD.

  25. Comparative Transcriptome Analysis of Grafted Tomato with Drought Tolerance International-journal

    Maria Isabel Fuentes-Merlos, Masaru Bamba, Shusei Sato, Atsushi Higashitani

    Plants　11　(15)　1947-1947　2022/07/27

    Publisher: MDPI AG

    DOI： 10.3390/plants11151947 　

    eISSN： 2223-7747

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    Grafting is a method used in agriculture to improve crop production and tolerance to biotic and abiotic stress. This technique is widely used in tomato, Solanum lycopersicum L.; however, the effects of grafting on changes in gene expression associated with stress tolerance in shoot apical meristem cells are still under-discovered. To clarify the effect of grafting, we performed a transcriptomic analysis between non-grafted and grafted tomatoes using the tomato variety Momotaro-scion and rootstock varieties, TD1, GS, and GF. Drought tolerance was significantly improved not only by a combination of compatible resistant rootstock TD1 but also by self-grafted compared to non-grafted lines. Next, we found the differences in gene expression between grafted and non-grafted plants before and during drought stress treatment. These altered genes are involved in the regulation of plant hormones, stress response, and cell proliferation. Furthermore, when comparing compatible (Momo/TD1 and Momo/Momo) and incompatible (Momo/GF) grafted lines, the incompatible line reduced gene expression associated with phytohormones but increased in wounding and starvation stress-response genes. These results conclude that grafting generates drought stress tolerance through several gene expression changes in the apical meristem.

  26. Phenolic Acids Induce Nod Factor Production in Lotus japonicus-Mesorhizobium Symbiosis.

    Masayuki Shimamura, Takashi Kumaki, Shun Hashimoto, Kazuhiko Saeki, Shin-Ichi Ayabe, Atsushi Higashitani, Tomoyoshi Akashi, Shusei Sato, Toshio Aoki

    Microbes and environments　37　(1)　n/a-n/a　2022

    Publisher: Japanese Society of Microbial Ecology

    DOI： 10.1264/jsme2.ME21094 　

    ISSN： 1342-6311

    eISSN： 1347-4405

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    In legume-rhizobia symbiosis, partner recognition and the initiation of symbiosis processes require the mutual exchange of chemical signals. Chemicals, generally (iso)flavonoids, in the root exudates of the host plant induce the expression of nod genes in rhizobia, and, thus, are called nod gene inducers. The expression of nod genes leads to the production of lipochitooligosaccharides (LCOs) called Nod factors. Natural nod gene inducer(s) in Lotus japonicus-Mesorhizobium symbiosis remain unknown. Therefore, we developed an LCO detection method based on ultra-high-performance liquid chromatography-tandem-quadrupole mass spectrometry (UPLC-TQMS) to identify these inducers and used it herein to screen 40 phenolic compounds and aldonic acids for their ability to induce LCOs in Mesorhizobium japonicum MAFF303099. We identified five phenolic acids with LCO-inducing activities, including p-coumaric, caffeic, and ferulic acids. The induced LCOs caused root hair deformation, and nodule numbers in L. japonicus inoculated with M. japonicum were increased by these phenolic acids. The three phenolic acids listed above induced the expression of the nodA, nodB, and ttsI genes in a strain harboring a multicopy plasmid encoding NodD1, but not that encoding NodD2. The presence of p-coumaric and ferulic acids in the root exudates of L. japonicus was confirmed by UPLC-TQMS, and the induction of ttsI::lacZ in the strain harboring the nodD1 plasmid was detected in the rhizosphere of L. japonicus. Based on these results, we propose that phenolic acids are a novel type of nod gene inducer in L. japonicus-Mesorhizobium symbiosis.

  27. Histone deacetylase HDA-4-mediated epigenetic regulation in space-flown C. elegans. International-journal

    Atsushi Higashitani, Toko Hashizume, Mai Takiura, Nahoko Higashitani, Mika Teranishi, Rika Oshima, Sachiko Yano, Kana Kuriyama, Akira Higashibata

    NPJ microgravity　7　(1)　33-33　2021/09/01

    DOI： 10.1038/s41526-021-00163-7 　

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    Epigenetic changes during long-term spaceflight are beginning to be studied by NASA's twin astronauts and other model organisms. Here, we evaluate the epigenetic regulation of gene expression in space-flown C. elegans by comparing wild type and histone deacetylase (hda)-4 mutants. Expression levels of 39 genes were consistently upregulated in all four generations of adult hda-4 mutants grown under microgravity compared with artificial Earth-like gravity (1G). In contrast, in the wild type, microgravity-induced upregulation of these genes occurred a little. Among these genes, 11 contain the domain of unknown function 19 (DUF-19) and are located in a cluster on chromosome V. When compared with the 1G condition, histone H3 trimethylation at lysine 27 (H3K27me3) increased under microgravity in the DUF-19 containing genes T20D4.12 to 4.10 locus in wild-type adults. On the other hand, this increase was also observed in the hda-4 mutant, but the level was significantly reduced. The body length of wild-type adults decreased slightly but significantly when grown under microgravity. This decrease was even more pronounced with the hda-4 mutant. In ground-based experiments, one of the T20D4.11 overexpressing strains significantly reduced body length and also caused larval growth retardation and arrest. These results indicate that under microgravity, C. elegans activates histone deacetylase HDA-4 to suppress overregulation of several genes, including the DUF-19 family. In other words, the expression of certain genes, including negative regulators of growth and development, is epigenetically fine-tuned to adapt to the space microgravity.

  28. Morin attenuates dexamethasone-mediated oxidative stress and atrophy in mouse C2C12 skeletal myotubes. International-journal

    Anayt Ulla, Takayuki Uchida, Yukari Miki, Kosuke Sugiura, Atsushi Higashitani, Takeshi Kobayashi, Ayako Ohno, Reiko Nakao, Katsuya Hirasaka, Iori Sakakibara, Takeshi Nikawa

    Archives of biochemistry and biophysics　704　108873-108873　2021/06/15

    DOI： 10.1016/j.abb.2021.108873 　

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    Glucocorticoids are the drugs most commonly used to manage inflammatory diseases. However, they are prone to inducing muscle atrophy by increasing muscle proteolysis and decreasing protein synthesis. Various studies have demonstrated that antioxidants can mitigate glucocorticoid-induced skeletal muscle atrophy. Here, we investigated the effect of a potent antioxidative natural flavonoid, morin, on the muscle atrophy and oxidative stress induced by dexamethasone (Dex) using mouse C2C12 skeletal myotubes. Dex (10 μM) enhanced the production of reactive oxygen species (ROS) in C2C12 myotubes via glucocorticoid receptor. Moreover, Dex administration reduced the diameter and expression levels of the myosin heavy chain protein in C2C12 myotubes, together with the upregulation of muscle atrophy-associated ubiquitin ligases, such as muscle atrophy F-box protein 1/atrogin-1, muscle ring finger protein-1, and casitas B-lineage lymphoma proto-oncogene-b. Dex also significantly decreased phosphorylated Foxo3a and increased total Foxo3a expression. Interestingly, Dex-induced ROS accumulation and Foxo3a expression were inhibited by morin (10 μM) pretreatment. Morin also prevented the Dex-induced reduction of myotube thickness, together with muscle protein degradation and suppression of the upregulation of atrophy-associated ubiquitin ligases. In conclusion, our results suggest that morin effectively prevents glucocorticoid-induced muscle atrophy by reducing oxidative stress.

  29. Findings from recent studies by the Japan Aerospace Exploration Agency examining musculoskeletal atrophy in space and on Earth. International-journal

    Satoshi Furukawa, Masahiro Chatani, Atsushi Higashitani, Akira Higashibata, Fuminori Kawano, Takeshi Nikawa, Takuro Numaga-Tomita, Toshihiko Ogura, Fuminori Sato, Atsuko Sehara-Fujisawa, Masahiro Shinohara, Toru Shimazu, Satoru Takahashi, Haruko Watanabe-Takano

    NPJ microgravity　7　(1)　18-18　2021/05/26

    DOI： 10.1038/s41526-021-00145-9 　

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    The musculoskeletal system provides the body with correct posture, support, stability, and mobility. It is composed of the bones, muscles, cartilage, tendons, ligaments, joints, and other connective tissues. Without effective countermeasures, prolonged spaceflight under microgravity results in marked muscle and bone atrophy. The molecular and physiological mechanisms of this atrophy under unloaded conditions are gradually being revealed through spaceflight experiments conducted by the Japan Aerospace Exploration Agency using a variety of model organisms, including both aquatic and terrestrial animals, and terrestrial experiments conducted under the Living in Space project of the Japan Ministry of Education, Culture, Sports, Science, and Technology. Increasing our knowledge in this field will lead not only to an understanding of how to prevent muscle and bone atrophy in humans undergoing long-term space voyages but also to an understanding of countermeasures against age-related locomotive syndrome in the elderly.

  30. Combined Environment Simulator for Low-Dose-Rate Radiation and Partial Gravity of Moon and Mars International-journal

    Akihisa Takahashi, Sakuya Yamanouchi, Kazuomi Takeuchi, Shogo Takahashi, Mutsumi Tashiro, Jun Hidema, Atsushi Higashitani, Takuya Adachi, Shenke Zhang, Fady Nagy Lotfy Guirguis, Yukari Yoshida, Aiko Nagamatsu, Megumi Hada, Kunihito Takeuchi, Tohru Takahashi, Yuji Sekitomi

    Life　10　(11)　274-274　2020/11/06

    Publisher: MDPI AG

    DOI： 10.3390/life10110274 　

    eISSN： 2075-1729

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    Deep space exploration by humans has become more realistic, with planned returns to the Moon, travel to Mars, and beyond. Space radiation with a low dose rate would be a constant risk for space travelers. The combined effects of space radiation and partial gravity such as on the Moon and Mars are unknown. The difficulty for such research is that there are no good simulating systems on the ground to investigate these combined effects. To address this knowledge gap, we developed the Simulator of the environments on the Moon and Mars with Neutron irradiation and Gravity change (SwiNG) for in vitro experiments using disposable closed cell culture chambers. The device simulates partial gravity using a centrifuge in a three-dimensional clinostat. Six samples are exposed at once to neutrons at a low dose rate (1 mGy/day) using Californium-252 in the center of the centrifuge. The system is compact including two SwiNG devices in the incubator, one with and one without radiation source, with a cooling function. This simulator is highly convenient for ground-based biological experiments because of limited access to spaceflight experiments. SwiNG can contribute significantly to research on the combined effects of space radiation and partial gravity.

  31. Ultra-high-purity iron is a novel and very compatible biomaterial. International-journal Peer-reviewed

    Luqman Khan, Katsumi Sato, Shinichi Okuyama, Takeshi Kobayashi, Kazumasa Ohashi, Katsuya Hirasaka, Takeshi Nikawa, Kunio Takada, Atsushi Higashitani, Kenji Abiko

    Journal of the mechanical behavior of biomedical materials　106　103744-103744　2020/03/27

    DOI： 10.1016/j.jmbbm.2020.103744 　

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    Metals and alloys are used widely in bone prosthetic materials, stents and dental tissue reconstructions. The most common materials are stainless steels and cobalt-chromium-nickel and titanium alloys. These alloys can be easily deformed but are hard to break. However, their affinity for cells and tissues is very low. In addition, they can sometimes provoke unexpected metal allergies. Iron is an abundant trace element essential for humans. However, excess amounts in particular of Fe2+ ions are toxic. We previously succeeded in obtaining 99.9996% ultra-high-purity iron (ABIKO iron). The chemical properties of ABIKO iron are completely different from that of conventional pure iron. For example, the reaction rate in hydrochloric acid is very slow and there is barely any corrosion. Here, we found that, in the absence of any type of coating, mammalian cells could easily attach to, and normally proliferate and differentiate on, ABIKO iron. On the other hand, cell densities and proliferation rate of the surfaces of plates made from Co-Cr-Mo or Ti-6Al-4V were significantly reduced. In addition, several stress and iron response genes, HSP70, SOD1, ATM and IRP2 did not change in the cells on ABIKO iron, while these genes were induced with exogenous application of FeSO4. Cells also secreted and fastened some organics on ABIKO iron. In vitro collagen binding assay showed that ABIKO iron binds higher amount of collagens. These findings highlight ABIKO iron as a novel biocompatible prosthetic material.

  32. Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C. elegans. International-journal Peer-reviewed

    Naoshi Watanabe, Masatoshi Morimatsu, Ayano Fujita, Mika Teranishi, Surabhi Sudevan, Masaru Watanabe, Hiroaki Iwasa, Yutaka Hata, Hiroyuki Kagi, Masayoshi Nishiyama, Keiji Naruse, Atsushi Higashitani

    Biochemical and biophysical research communications　523　(4)　853-858　2020/03/19

    DOI： 10.1016/j.bbrc.2020.01.047 　

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    Mechanical stimulation is well known to be important for maintaining tissue and organ homeostasis. Here, we found that hydrostatic pressure induced nuclear translocation of a forkhead box O (FOXO) transcription factor DAF-16, in C. elegans within minutes, whereas the removal of this pressure resulted in immediate export of DAF-16 to the cytoplasm. We also monitored DAF-16-dependent transcriptional changes by exposure to 1 MPa pressure for 5 min, and found significant changes in collagen and other genes in a DAF-16 dependent manner. Lifespan was markedly prolonged with exposure to cyclic pressure treatment (1 MPa once a day for 5 min from L1 larvae until death). Furthermore, age-dependent decline in locomotor activity was suppressed by the treatment. In contrast, the nuclear translocation of the yes-associated protein YAP-1 was not induced under the same pressure conditions. Thus, moderate hydrostatic pressure improves ageing progression through activation of DAF-16/FOXO in C. elegans.

  33. Lotus Accessions Possess Multiple Checkpoints Triggered by Different Type III Secretion System Effectors of the Wide-Host-Range Symbiont Bradyrhizobium elkanii USDA61. Peer-reviewed

    Shohei Kusakabe, Nahoko Higasitani, Takakazu Kaneko, Michiko Yasuda, Hiroki Miwa, Shin Okazaki, Kazuhiko Saeki, Atsushi Higashitani, Shusei Sato

    Microbes and environments　35　(1)　2020

    DOI： 10.1264/jsme2.ME19141 　

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    Bradyrhizobium elkanii, a rhizobium with a relatively wide host range, possesses a functional type III secretion system (T3SS) that is involved in symbiotic incompatibility against Rj4-genotype soybean (Glycine max) and some accessions of mung bean (Vigna radiata). To expand our knowledge on the T3SS-mediated partner selection mechanism in the symbiotic legume-rhizobia association, we inoculated three Lotus experimental accessions with wild-type and T3SS-mutant strains of B. elkanii USDA61. Different responses were induced by T3SS in a host genotype-dependent manner. Lotus japonicus Gifu inhibited infection; L. burttii allowed infection, but inhibited nodule maturation at the post-infection stage; and L. burttii and L. japonicus MG-20 both displayed a nodule early senescence-like response. By conducting inoculation tests with mutants of previously reported and newly identified effector protein genes of B. elkanii USDA61, we identified NopF as the effector protein triggering the inhibition of infection, and NopM as the effector protein triggering the nodule early senescence-like response. Consistent with these results, the B. elkanii USDA61 gene for NopF introduced into the Lotus symbiont Mesorhizobium japonicum induced infection inhibition in L. japonicus Gifu, but did not induce any response in L. burttii or L. japonicus MG-20. These results suggest that Lotus accessions possess at least three checkpoints to eliminate unfavorable symbionts, including the post-infection stage, by recognizing different T3SS effector proteins at each checkpoint.

  34. Autophagy mitigates high-temperature injury in pollen development of Arabidopsis thaliana. International-journal Peer-reviewed

    Gönül Dündar, Zhenhua Shao, Nahoko Higashitani, Mami Kikuta, Masanori Izumi, Atsushi Higashitani

    Developmental biology　456　(2)　190-200　2019/12/15

    DOI： 10.1016/j.ydbio.2019.08.018 　

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    Autophagy is one of the cellular processes that break down cellular components during senescence, starvation, and stress. The susceptibility of plant pollen development to high-temperature (HT) stress is well known, but the involvement of autophagy in HT injury is yet to be clarified. Here, we found that following transfer to 30 °C, all autophagy-deficient (atg) mutants (atg2-1, 5-1, 7-2, and 10-1) of Arabidopsis thaliana tested displayed visibly impaired pollen development and anther dehiscence. HT-induced male sterility significantly increased in the atg mutants, but the degree of HT-induced obstacles did not change between the wild type (WT) and mutants from the seedling stage to the bolting stage. Cytological analyses showed that 30 °C promoted autophagy and autolysosome formation in both anther wall cells and microspores in developing anthers of WT, but the atg5-1 mutant did not show completion of tapetum degeneration and microspore maturation. HT upregulated hydrogen peroxide and dehydroascorbate reductase 1 production in both WT and atg5-1 anthers, but the basal levels were already higher in the mutant. HT repressed expression of UNDEAD and its regulator MYB80, which are required for tapetal programmed cell death (PCD) for proper pollen development. Taken together, our results suggest that autophagy functions in tapetum degeneration and pollen development during HT-caused tapetal PCD abortion.

  35. Vital roles of PCNA K165 modification during C. elegans gametogenesis and embryogenesis. International-journal Peer-reviewed

    Zhenhua Shao, Shinsuke Niwa, Atsushi Higashitani, Yasukazu Daigaku

    DNA repair　82　102688-102688　2019/10

    DOI： 10.1016/j.dnarep.2019.102688 　

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    In eukaryotes, the DNA damage bypass pathway is promoted by ubiquitylation of PCNA at the conserved lysine 164. Using CRISPR-Cas9 system, we introduced amino acid substitution at K165 of C. elegans PCNA that corresponds to K164 in other characterised organisms and examined the contribution of this residue at a variety of stages during development. In the presence of UV-induced DNA lesions, PCNA-K165 is crucial for not only the early embryonic stages but also during larval development, implicating its functions for a broad time period during animal development. We also show that, without induction of DNA damage, concomitant inhibition of PCNA ubiquitylation and checkpoint activation causes abnormal gametogenesis events and severely impairs reproduction of worms. Our findings suggest a conserved function of PCNA ubiquitylation in tolerance of UV-induced damage and also propose that PCNA ubiquitylation contributes to gametogenesis during unperturbed C. elegans development.

  36. ミトコンドリアを介した無重力ストレス感知機構の解明

    内田 貴之, 三木 裕加里, 坂下 禎宏, 真板 綾子, 中尾 玲子, 平坂 勝也, 東谷 篤志, 東端 晃, 小林 剛, 曽我部 正博, 二川 健

    日本栄養・食糧学会大会講演要旨集　73回　221-221　2019/04

    Publisher: (公社)日本栄養・食糧学会

  37. Target of rapamycin-signaling modulates starch accumulation via glycogenin phosphorylation status in the unicellular red alga Cyanidioschyzon merolae International-journal Peer-reviewed

    Pancha, I., Shima, H., Higashitani, N., Igarashi, K., Higashitani, A., Tanaka, K., Imamura, S.

    Plant Journal　97　(3)　485-499　2019/02

    DOI： 10.1111/tpj.14136 　

    ISSN： 1365-313X 0960-7412

  38. Divergent evolution of rice blast resistance Pi54 locus in the genus Oryza. International-journal Peer-reviewed

    Zhang L, Nakagomi Y, Endo T, Teranishi M, Hidema J, Sato S, Higashitani A

    Rice (New York, N.Y.)　11　(1)　63-63　2018/12

    DOI： 10.1186/s12284-018-0256-8 　

    ISSN： 1939-8425

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    BACKGROUND: The rice blast resistance gene Pi54 was cloned from Oryza sativa ssp. indica cv. Tetep, which conferred broad-spectrum resistance against Magnaporthe oryzae. Pi54 allelic variants have been identified in not only domesticates but also wild rice species, but the majority of japonica and some indica cultivars lost the function. RESULTS: We here found that Pi54 (Os11g0639100) and its homolog Os11g0640600 (named as #11) were closely located on a 25 kbp region in japonica cv. Sasanishiki compared to a 99 kbp region in japonica cv. Nipponbare. Sasanishiki lost at least six genes containing one other R-gene cluster (Os11g0639600, Os11g0640000, and Os11g0640300). Eight AA-genome species including five wild rice species were classified into either Nipponbare or Sasanishiki type. The BB-genome wild rice species O. punctata was Sasanishiki type. The FF-genome wild rice species O. brachyantha (the basal lineage of Oryza) was neither, because Pi54 was absent and the orientation of the R-gene cluster was reversed in comparison with Nipponbare-type species. The phylogenetic analysis showed that #11gene of O. brachyantha was on the root of both Pi54 and #11 alleles. All Nipponbare-type Pi54 alleles were specifically disrupted by 143 and 37/44 bp insertions compared to Tetep and Sasanishiki type. In addition, Pi54 of japonica cv. Sasanishiki lost nucleotide-binding site and leucine-rich repeat (NBS-LRR) domains owing to additional mutations. CONCLUSIONS: These results suggest that Pi54 might be derived from a tandem duplication of the ancestor #11 gene in progenitor FF-genome species. Two divergent structures of Pi54 locus caused by a mobile unit containing the nearby R-gene cluster could be developed before domestication. This study provides a potential genetic resource of rice breeding for blast resistance in modern cultivars sustainability.

  39. モデル生物「線虫」を用いた放射線ストレス応答研究の新展開 線虫C.elegansのDAF-16/FoxOを介した新たな静水圧応答

    渡辺 尚, 森松 賢順, 西山 雅祥, 渡邉 賢, 鍵 裕之, 成瀬 恵治, 東谷 篤志

    日本放射線影響学会大会講演要旨集　61回　36-36　2018/11

    Publisher: (一社)日本放射線影響学会

    ISSN： 1347-8680

  40. Morin suppresses cachexia-induced muscle wasting by binding to ribosomal protein S10 in carcinoma cells International-journal Peer-reviewed

    Yoshimura, T., Saitoh, K., Sun, L., Wang, Y., Taniyama, S., Yamaguchi, K., Uchida, T., Ohkubo, T., Higashitani, A., Nikawa, T., Tachibana, K., Hirasaka, K.

    Biochemical and Biophysical Research Communications　506　(4)　773-779　2018/10/30

    DOI： 10.1016/j.bbrc.2018.10.184 　

    ISSN： 1090-2104 0006-291X

  41. Reactive oxygen species upregulate expression of muscle atrophy-associated ubiquitin ligase Cbl-b in rat L6 skeletal muscle cells International-journal Peer-reviewed

    Takayuki Uchida, Yoshihiro Sakashita, Kanako Kitahata, Yui Yamashita, Chisato Tomida, Yuki Kimori, Akio Komatsu, Katsuya Hirasaka, Ayako Ohno, Reiko Nakao, Atsushi Higashitani, Akira Higashibata, Noriaki Ishioka, Toru Shimazu, Takeshi Kobayashi, Yuushi Okumura, Inho Choi, Motoko Oarada, Edward M. Mills, Shigetada Teshima-Kondo, Shin’ichi Takeda, Eiji Tanaka, Keiji Tanaka, Masahiro Sokabe, Takeshi Nikawa

    American Journal of Physiology - Cell Physiology　314　(6)　C721-C731　2018/06/01

    Publisher: American Physiological Society

    DOI： 10.1152/ajpcell.00184.2017 　

    ISSN： 1522-1563 0363-6143

  42. 無重力による廃用性筋萎縮における酸化ストレスの重要性

    内田 貴之, 木森 有希, 坂下 禎宏, 真板 綾子, 中尾 玲子, 平坂 勝也, 東谷 篤志, 東端 晃, 小林 剛, 曽我部 正博, 武田 伸一, 二川 健

    日本栄養・食糧学会大会講演要旨集　72回　187-187　2018/04

    Publisher: (公社)日本栄養・食糧学会

  43. Heat-Induced Calcium Leakage Causes Mitochondrial Damage in Caenorhabditis elegans Body-Wall Muscles International-journal Peer-reviewed

    Kenta Momma, Takashi Homma, Ruri Isaka, Surabhi Sudevan, Atsushi Higashitani

    GENETICS　206　(4)　1985-1994　2017/08

    DOI： 10.1534/genetics.117.202747 　

    ISSN： 0016-6731

    eISSN： 1943-2631

  44. Dietary Supplementation with Isoflavones Prevents Muscle Wasting in Tumor-Bearing Mice Peer-reviewed

    Katsuya Hirasaka, Shinobu Saito, Saki Yamaguchi, Riho Miyazaki, Yao Wang, Marie Haruna, Shigeto Taniyama, Atsushi Higashitani, Junji Terao, Takeshi Nikawa, Katsuyasu Tachibana

    JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY　62　(3)　178-184　2016/06

    DOI： 10.3177/jnsv.62.178 　

    ISSN： 0301-4800

    eISSN： 1881-7742

  45. Fluid dynamics alter Caenorhabditis elegans body length via TGF-beta/DBL-1 neuromuscular signaling International-journal Peer-reviewed

    Shunsuke Harada, Toko Hashizume, Kanako Nemoto, Zhenhua Shao, Nahoko Higashitani, Timothy Etheridge, Nathaniel J. Szewczyk, Keiji Fukui, Akira Higashibata, Atsushi Higashitani

    NPJ MICROGRAVITY　2　16006-16006　2016/04

    DOI： 10.1038/npjmgrav.2016.6 　

    ISSN： 2373-8065

  46. Microgravity elicits reproducible alterations in cytoskeletal and metabolic gene and protein expression in space-flown Caenorhabditis elegans International-journal Peer-reviewed

    Akira Higashibata, Toko Hashizume, Kanako Nemoto, Nahoko Higashitani, Timothy Etheridge, Chihiro Mori, Shunsuke Harada, Tomoko Sugimoto, Nathaniel J. Szewczyk, Shoji A. Baba, Yoshihiro Mogami, Keiji Fukui, Atsushi Higashitani

    NPJ MICROGRAVITY　2　15022-15022　2016/01

    DOI： 10.1038/npjmgrav.2015.22 　

    ISSN： 2373-8065

  47. High and low temperature injuries to microsporogenesis by decreasing phytohormones, auxin and gibberellin Invited Peer-reviewed

    Atsushi Higashitani

    Regulation of Plant Growth and Development　50　(2)　162-165　2015/12/21

  48. Fine mapping of a major quantitative trait locus, qLG-9, that controls seed longevity in rice (Oryza sativa L.) Peer-reviewed

    K. Sasaki, Y. Takeuchi, K. Miura, T. Yamaguchi, T. Ando, T. Ebitani, A. Higashitani, T. Yamaya, M. Yano, T. Sato

    THEORETICAL AND APPLIED GENETICS　128　(4)　769-778　2015/04

    DOI： 10.1007/s00122-015-2471-7 　

    ISSN： 0040-5752

    eISSN： 1432-2242

  49. Caenorhabditis elegans ATR checkpoint kinase ATL-1 influences life span through mitochondrial maintenance International-journal Peer-reviewed

    Kazuhiro Suetomi, Stefan Mereiter, Chihiro Mori, Takako Takanami, Atsushi Higashitani

    MITOCHONDRION　13　(6)　729-735　2013/11

    DOI： 10.1016/j.mito.2013.02.004 　

    ISSN： 1567-7249

    eISSN： 1872-8278

  50. Over-expression of ATR causes autophagic cell death Peer-reviewed

    Chihiro Mori, Yoshihiro Yamaguchi, Mika Teranishi, Takako Takanami, Takahiro Nagase, Shinichiro Kanno, Akira Yasui, Atsushi Higashitani

    Genes to Cells　18　(4)　278-287　2013/04

    DOI： 10.1111/gtc.12034 　

    ISSN： 1356-9597 1365-2443

  51. High temperature injury and auxin biosynthesis in microsporogenesis International-journal Peer-reviewed

    Atsushi Higashitani

    FRONTIERS IN PLANT SCIENCE　4　47-47　2013/03

    DOI： 10.3389/fpls.2013.00047 　

    ISSN： 1664-462X

  52. Isolation of a novel mutant gene for soil-surface rooting in rice (Oryza sativa L.) International-journal Peer-reviewed

    Eiko Hanzawa, Kazuhiro Sasaki, Shinsei Nagai, Mitsuhiro Obara, Yoshimichi Fukuta, Yusaku Uga, Akio Miyao, Hirohiko Hirochika, Atsushi Higashitani, Masahiko Maekawa, Tadashi Sato

    Rice　6　(1)　1-11　2013

    DOI： 10.1186/1939-8433-6-30 　

    ISSN： 1939-8425

    eISSN： 1939-8433

  53. Innate Immune Genes Including a Mucin-Like Gene, mul-1, Induced by Ionizing Radiation in Caenorhabditis elegans Peer-reviewed

    Takafumi Kimura, Takako Takanami, Tetsuya Sakashita, Seiichi Wada, Yasuhiko Kobayashi, Atsushi Higashitani

    RADIATION RESEARCH　178　(4)　313-320　2012/10

    DOI： 10.1667/RR2989.1 　

    ISSN： 0033-7587

  54. CDC-48/p97 is required for proper meiotic chromosome segregation via controlling AIR-2/Aurora B kinase localization in Caenorhabditis elegans Peer-reviewed

    Yohei Sasagawa, Atsushi Higashitani, Takeshi Urano, Teru Ogura, Kunitoshi Yamanaka

    JOURNAL OF STRUCTURAL BIOLOGY　179　(2)　104-111　2012/08

    DOI： 10.1016/j.jsb.2012.06.009 　

    ISSN： 1047-8477

    eISSN： 1095-8657

  55. Genes down-regulated in spaceflight are involved in the control of longevity in Caenorhabditis elegans Peer-reviewed

    Yoko Honda, Akira Higashibata, Yohei Matsunaga, Yukiko Yonezawa, Tsuyoshi Kawano, Atsushi Higashitani, Kana Kuriyama, Toru Shimazu, Masashi Tanaka, Nathaniel J. Szewczyk, Noriaki Ishioka, Shuji Honda

    SCIENTIFIC REPORTS　2　487-487　2012/07

    DOI： 10.1038/srep00487 　

    ISSN： 2045-2322

  56. Impaired development of melanoblasts in the black-eyed white Mitfmi-bw mouse, a model for auditory-pigmentary disorders Peer-reviewed

    Hiroki Hozumi, Kazuhisa Takeda, Yasuko Yoshida-Amano, Yuji Takemoto, Ryota Kusumi, Urara Fukuzaki-Dohi, Atsushi Higashitani, Hiroaki Yamamoto, Shigeki Shibahara

    GENES TO CELLS　17　(6)　494-508　2012/06

    DOI： 10.1111/j.1365-2443.2012.01603.x 　

    ISSN： 1356-9597

  57. Ag 2O 3 clathrate is a novel and effective antimicrobial agent Peer-reviewed

    Saaya Ando, Tomosato Hioki, Takamichi Yamada, Naoshi Watanabe, Atsushi Higashitani

    Journal of Materials Science　47　(6)　2928-2931　2012/03

    DOI： 10.1007/s10853-011-6125-0 　

    ISSN： 0022-2461 1573-4803

  58. Excellent thermal control ability of Cell Biology Experimental Facility (CBEF) for ground-based experiments and experiments onboard the Kibo Japanese Experiment Module of International Space Station Peer-reviewed

    Yano S, Masuda D, Kasahara H, Omori K, Higashibata A, Asashima M, Ohnishi T, Yatagai F, Kamisaka S, Furusawa T, Higashitani A, Majima HJ, Nikawa T, Wakabayashi K, Takahashi H, Suzuki H, Shimazu T, Fukui K, Hattori A, Tanigaki F, Shirakawa M, Nakamura T, Yoshimura Y, Suzuki N, Ishioka N

    Biological Sciences in Space　26　12-20　2012

  59. Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.) International-journal Peer-reviewed

    Hyun-Sook Lee, Kazuhiro Sasaki, Atsushi Higashitani, Sang-Nag Ahn, Tadashi Sato

    RICE　5　(1)　13-13　2012

    DOI： 10.1186/1939-8433-5-13 　

    ISSN： 1939-8425

    eISSN： 1939-8433

  60. The next phase of life-sciences spaceflight research: Harnessing the power of functional genomics. Peer-reviewed

    Etheridge T, Nemoto K, Hashizume T, Mori C, Sugimoto T, Suzuki H, Fukui K, Yamazaki T, Higashibata A, Szewczyk NJ, Higashitani A

    Communicative & integrative biology　4　(6)　668-669　2011/11

    Publisher: Informa UK Limited

    DOI： 10.4161/cib.16975 　

    eISSN： 1942-0889

  61. The Effectiveness of RNAi in Caenorhabditis elegans Is Maintained during Spaceflight Peer-reviewed

    Timothy Etheridge, Kanako Nemoto, Toko Hashizume, Chihiro Mori, Tomoko Sugimoto, Hiromi Suzuki, Keiji Fukui, Takashi Yamazaki, Akira Higashibata, Nathaniel J. Szewczyk, Atsushi Higashitani

    PLOS ONE　6　(6)　e20459-e20459　2011/06

    DOI： 10.1371/journal.pone.0020459 　

    ISSN： 1932-6203

  62. 植物の高温障害による雄性不稔とオーキシン : 葯特異的にシグナル伝達活性が低下

    阪田 忠, 東谷 篤志

    化学と生物　49　(3)　152-154　2011/03/01

    Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry

    DOI： 10.1271/kagakutoseibutsu.49.152 　

    ISSN： 0453-073X

  63. Auxin depletion in barley plants under high-temperature conditions represses DNA proliferation in organelles and nuclei via transcriptional alterations Peer-reviewed

    Takeshi Oshino, Shinya Miura, Syunsuke Kikuchi, Kazuki Hamada, Kentaro Yano, Masao Watanabe, Atsushi Higashitani

    PLANT CELL AND ENVIRONMENT　34　(2)　284-290　2011/02

    DOI： 10.1111/j.1365-3040.2010.02242.x 　

    ISSN： 0140-7791

  64. Radiation Biology of Caenorhabditis elegans: Germ Cell Response, Aging and Behavior Peer-reviewed

    Tetsuya Sakashita, Takako Takanami, Sumino Yanase, Nobuyuki Hamada, Michiyo Suzuki, Takafumi Kimura, Yasuhiko Kobayashi, Naoaki Ishii, Atsushi Higashitani

    JOURNAL OF RADIATION RESEARCH　51　(2)　107-121　2010/03

    DOI： 10.1269/jrr.09100 　

    ISSN： 0449-3060

    eISSN： 1349-9157

  65. Tissue-specific auxin signaling in response to temperature fluctuation Invited Peer-reviewed

    Tadashi Sakata, Nao Yagihashi, Atsushi Higashitani

    Plant Signaling and Behavior　5　(11)　1510-1511　2010

    Publisher: Landes Bioscience

    DOI： 10.4161/psb.5.11.13706 　

    ISSN： 1559-2324 1559-2316

  66. High Temperatures Cause Male Sterility in Rice Plants with Transcriptional Alterations During Pollen Development Peer-reviewed

    Makoto Endo, Tohru Tsuchiya, Kazuki Hamada, Shingo Kawamura, Kentaro Yano, Masahiro Ohshima, Atsushi Higashitani, Masao Watanabe, Makiko Kawagishi-Kobayashi

    PLANT AND CELL PHYSIOLOGY　50　(11)　1911-1922　2009/11

    DOI： 10.1093/pcp/pcp135 　

    ISSN： 0032-0781

  67. C. elegans RNAi space experiment (CERISE) in Japanese Experiment Module KIBO. Peer-reviewed

    Higashitani A, Hashizume T, Sugimoto, T, Mori C Nemoto, K, Etheridge T, Higashitani, N, Takanami T, Suzuki H Fukui, K, Yamazaki T, Ishioka N Szewczyk, N, Higashibata A

    Biol Sci Space　23　(4)　183-187　2009/10/01

    Publisher: Japanese Society for Biological Sciences in Space

    DOI： 10.2187/bss.23.183 　

    ISSN： 0914-9201

    eISSN： 1349-967X

  68. Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner Peer-reviewed

    Yohei Sasagawa, Mieko Otani, Nahoko Higashitani, Atsushi Higashitani, Ken Sato, Teru Ogura, Kunitoshi Yamanaka

    JOURNAL OF CELL SCIENCE　122　(20)　3663-3672　2009/10

    DOI： 10.1242/jcs.052415 　

    ISSN： 0021-9533

  69. Localization of X-ray cross complementing gene 1 protein in the nuclear matrix is controlled by casein kinase II-dependent phosphorylation in response to oxidative damage Peer-reviewed

    Yoshiko Kubota, Takako Takanami, Atsushi Higashitani, Saburo Horiuchi

    DNA REPAIR　8　(8)　953-960　2009/08

    DOI： 10.1016/j.dnarep.2009.06.003 　

    ISSN： 1568-7864

  70. Effects of Ionizing Radiation on Locomotory Behavior and Mechanosensation in Caenorhabditis elegans Peer-reviewed

    Michiyo Suzuki, Tetsuya Sakashita, Sumino Yanase, Masahiro Kikuchi, Hirofumi Ohba, Atsushi Higashitani, Nobuyuki Hamada, Tomoo Funayama, Kana Fukamoto, Toshio Tsuji, Yasuhiko Kobayashi

    JOURNAL OF RADIATION RESEARCH　50　(2)　119-125　2009/03

    DOI： 10.1269/jrr.08087 　

    ISSN： 0449-3060

  71. Nitrous Oxide Emission and Microbial Community in the Rhizosphere of Nodulated Soybeans during the Late Growth Period Peer-reviewed

    Shoko Inaba, Kenji Tanabe, Shima Eda, Seishi Ikeda, Atsushi Higashitani, Hisayuki Mitsui, Kiwamu Minamisawa

    MICROBES AND ENVIRONMENTS　24　(1)　64-67　2009

    DOI： 10.1264/jsme2.ME08544 　

    ISSN： 1342-6311

  72. The Effect of High Strength Static Magnetic Fields and Ionizing Radiation on Gene Expression and DNA Damage in Caenorhabditis elegans Peer-reviewed

    Takafumi Kimura, Kohki Takahashi, Yoko Suzuki, Yukinaga Konishi, Yuta Ota, Chihiro Mori, Takahiko Ikenaga, Takako Takanami, Rumiko Saito, Eiichiro Ichiishi, Satoshi Awaji, Kazuo Watanabe, Atsushi Higashitani

    BIOELECTROMAGNETICS　29　(8)　605-614　2008/12

    DOI： 10.1002/bem.20425 　

    ISSN： 0197-8462

  73. Maintenance of mitochondrial DNA by the Caenorhabditis elegans ATR checkpoint protein ATL-1 Peer-reviewed

    Chihiro Mori, Takako Takanami, Atsushi Higashitani

    GENETICS　180　(1)　681-686　2008/09

    DOI： 10.1534/genetics.108.090704 　

    ISSN： 0016-6731

    eISSN： 1943-2631

  74. Description of international Caenorhabditis elegans experiment first flight (ICE-FIRST) Peer-reviewed

    N. J. Szewczyk, J. Tillman, C. A. Conley, L. Granger, L. Segalat, A. Higashitani, S. Honda, Y. Honda, H. Kagawa, R. Adachi, A. Higashibata, N. Fujimoto, K. Kuriyama, N. Ishioka, K. Fukui, D. Baillie, A. Rose, G. Gasset, B. Eche, D. Chaput, M. Viso

    ADVANCES IN SPACE RESEARCH　42　(6)　1072-1079　2008/09

    DOI： 10.1016/j.asr.2008.03.017 　

    ISSN： 0273-1177

    eISSN： 1879-1948

  75. Involvement of HMG-12 and CAR-1 in the cdc-48.1 expression of Caenorhabditis elegans Peer-reviewed

    Seiji Yamauchi, Nahoko Higashitani, Mieko Otani, Atsushi Higashitani, Teru Ogura, Kunitoshi Yamanaka

    DEVELOPMENTAL BIOLOGY　318　(2)　348-359　2008/06

    DOI： 10.1016/j.ydbio.2008.02.057 　

    ISSN： 0012-1606

  76. Caenorhabditis elegans par2.1/mtssb-1 is essential for mitochondrial DNA replication and its defect causes comprehensive transcriptional alterations including a hypoxia response Peer-reviewed

    Tomoko Sugimoto, Chihiro Mori, Takako Takanami, Yohei Sasagawa, Rumiko Saito, Eiichiro Ichiishi, Atsushi Higashitani

    EXPERIMENTAL CELL RESEARCH　314　(1)　103-114　2008/01

    DOI： 10.1016/j.yexcr.2007.08.015 　

    ISSN： 0014-4827

  77. Genomic response of the nematode Caenorhabditis elegans to spaceflight Peer-reviewed

    Florian Selch, Akira Higashibata, Mari Imamizo-Sato, Atsushi Higashitani, Noriaki Ishioka, Nathaniel J. Szewczyk, Catharine A. Conley

    ADVANCES IN SPACE RESEARCH　41　(5)　807-815　2008

    DOI： 10.1016/j.asr.2007.11.015 　

    ISSN： 0273-1177

  78. Premature progression of anther early developmental programs accompanied by comprehensive alterations in transcription during high-temperature injury in barley plants Peer-reviewed

    Takeshi Oshino, Mafumi Abiko, Rumiko Saito, Eiichiro Ichiishi, Makoto Endo, Makiko Kawagishi-Kobayashi, Atsushi Higashitani

    MOLECULAR GENETICS AND GENOMICS　278　(1)　31-42　2007/07

    DOI： 10.1007/s00438-007-0229-x 　

    ISSN： 1617-4615

  79. A gene essential for hydrotropism in roots Peer-reviewed

    Akie Kobayashi, Akiko Takahashi, Yoko Kakimoto, Yutaka Miyazawa, Nobuharu Fujii, Atsushi Higashitani, Hideyuki Takahashi

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　104　(11)　4724-4729　2007/03

    DOI： 10.1073/pnas.0609929104 　

    ISSN： 0027-8424

  80. C-elegans RBX-2-CUL-5- and RBX-1-CUL-2-based complexes are redundant for oogenesis and activation of the MAP kinase MPK-1 Peer-reviewed

    Yohei Sasagawa, Shusei Sato, Teru Ogura, Atsushi Higashitani

    FEBS LETTERS　581　(1)　145-150　2007/01

    DOI： 10.1016/j.febslet.2006.12.009 　

    ISSN： 0014-5793

  81. Biochemical and Molecular Biological Analyses of space-flown nematodes in Japan, the First International Caenorhabditis elegans Experiment (ICE-First) Invited Peer-reviewed

    Akira Higashibata, Atsushi Higashitani, Ryota Adachi, Hiroaki Kagawa, Shuji Honda, Yoko Honda, Nahoko Higashitani, Yohei Sasagawa, Yutaka Miyazawa, Nathaniel J. Szewczyk, Catharine A. Conley, Nobuyoshi Fujimoto, Keiji Fukui, Toru Shimazu, Kana Kuriyama, Noriaki Ishioka

    MICROGRAVITY SCIENCE AND TECHNOLOGY　19　(5-6)　159-163　2007

    DOI： 10.1007/BF02919473 　

    ISSN： 0938-0108

  82. マイクロビームを用いた研究の進展 重イオンマイクロビームを用いた線虫Cエレガンスの放射線応答の研究

    東谷 篤志, 森 ちひろ, 杉本 朋子, 太齋 久美子, 坂下 哲哉, 舟山 知夫, 柿崎 竹彦, 浜田 信行, 和田 成一, 小林 泰彦

    日本放射線影響学会大会講演要旨集　49回　84-84　2006/09

    Publisher: (一社)日本放射線影響学会

    ISSN： 1347-8680

  83. モデル生物線虫Cエレガンスの生殖細胞における放射線応答の研究

    森 ちひろ, 池永 貴彦, 杉本 朋子, 太齋 久美子, 坂下 哲哉, 舟山 知夫, 柿崎 竹彦, 浜田 信行, 和田 成一, 小林 泰彦, 一石 英一郎, 齋藤 るみ子, 東谷 篤志

    日本放射線影響学会大会講演要旨集　49回　130-130　2006/09

    Publisher: (一社)日本放射線影響学会

    ISSN： 1347-8680

  84. Decreased expression of myogenic transcription factors and myosin heavy chains in Caenorhabditis elegans muscles developed during spaceflight Peer-reviewed

    Akira Higashibata, Nathaniel J. Szewczyk, Catharine A. Conley, Mari Imamizo-Sato, Atsushi Higashitani, Noriaki Ishioka

    JOURNAL OF EXPERIMENTAL BIOLOGY　209　(16)　3209-3218　2006/08

    DOI： 10.1242/jeb.02365 　

    ISSN： 0022-0949

  85. Anther-specific genes, which expressed through microsporogenesis, are temporally and spatially regulated in model legume, Lotus japonicus Peer-reviewed

    Hiromi Masuko, Makoto Endo, Hiroshi Saito, Hirokazu Hakozaki, Jong-In Park, Makiko Kawagishi-Kobayashi, Yoshinobu Takada, Tomihiro Okabe, Motoshi Kamada, Hideyuki Takahashi, Atsushi Higashitani, Masao Watanabe

    GENES & GENETIC SYSTEMS　81　(1)　57-62　2006/02

    DOI： 10.1266/ggs.81.57 　

    ISSN： 1341-7568

    eISSN： 1880-5779

  86. Study of biological responses to ionizing radiation in the germline cells of C. elegans

    MORI Chihiro, IKENAGA Takahiko, SUGIMOTO Tomoko, DAZAI Kumiko, SAKASHITA Tetsuya, FUNAYAMA Tomoo, KAKIZAKI Takehiko, HAMADA Nobuyuki, WADA Seiichi, KOBAYASHI Yasuhiko, ICHIISHI Eiichiro, SAITO Rumiko, HIGASHITANI Atsushi

    The Japan Radiation Research Society Annual Meeting Abstracts　2006　215-215　2006

    Publisher: The Japanese Radiation Research Society

    DOI： 10.11513/jrrsabst.2006.0.215.0 　

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    Ionizing radiation causes DNA double-strand breaks. Organisms have a DNA repair system to maintain the integrity of genome. When DNA is severely damaged in Metazoa, apoptosis is induced to eliminate the damaged cells. A proto-oncogene c-Abl1 is involved in the positive regulation of DNA repair system in mammals. Furthermore the frequency of radiation-induced germline apoptosis increases in a C. elegans abl-1 deletion mutant. It indicates that another function is involved in the negative regulation of DNA damage-induced apoptosis. In this study, we carried out comprehensive analyses of gene expression after γ-ray irradiation in the wild-type and abl-1 adult hermaphrodites using the nematode DNA microarray. In the wild type, 1313 genes were up-regulated above twice 3 hr after irradiation. Especially 117 genes were up-regulated more than 1.4 times in the abl-1 deletion mutant irradiated as compared with the wild type. BH3-only protein gene egl-1 and ced-13 that are apoptosis-inducing signals were found in the 117 genes. In addition, certain genes of cytochrome P450 subfamily induced after irradiation in the wild type were remarkably repressed and not up-regulated in the abl-1 mutant. In this presentation, we also show the results of cell-cycle arrest, apoptosis, and little bystander effect in the germline cells following heavy-ion microbeam irradiation.

  87. Study of biological responses to heavy-ion microbeams in the nematode <I>C. elegans<I/>

    HIGASHITANI Atsushi, MORI Chihiro, SUGIMOTO Tomoko, DAZAI Kumiko, SAKASHITA Tetsuya, FUNAYAMA Tomoo, KAKIZAKI Takehiko, HAMADA Nobuyuki, WADA Seiichi, KOBAYASHI Yasuhiko

    The Japan Radiation Research Society Annual Meeting Abstracts　2006　79-79　2006

    Publisher: The Japanese Radiation Research Society

    DOI： 10.11513/jrrsabst.2006.0.79.0 　

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    The nematode Caenorhabditis elegans is an experimental model organism used to study several genetic systems for development, neuronal network, locomotion and reproduction. Its genome and cell lineage have been determined, and lots of genetic mutants have been isolated. We are now attempting to study the tissue-specific effects of ionizing radiation on the germline cells using the heavy-ion microbeam irradiation apparatus at TIARA. In this presentation, we show the experimental procedures and the results of cell-cycle arrest and apoptosis in the germline cells following heavy-ion microbeam irradiation, and discuss the future experiment

  88. Epistatic interaction of QTLs controlling leaf bronzing in rice (Oryza sativa L.) grown in a saline paddy field Peer-reviewed

    Hinako Takehisa, Tadamasa Ueda, Yoshimichi Fukuta, Mitsuhiro Obara, Tomoko Abe, Masahiro Yano, Tomoyuki Yamaya, Toshiaki Kameya, Atsushi Higashitani, Tadashi Sato

    Breeding Science　56　(3)　287-293　2006

    DOI： 10.1270/jsbbs.56.287 　

    ISSN： 1344-7610 1347-3735

  89. Cell cycle arrest and apoptosis in Caenorhabditis elegans germline cells following heavy-ion microbeam irradiation Peer-reviewed

    T Sugimoto, K Dazai, T Sakashita, T Funayama, S Wada, N Hamada, T Kakizaki, Y Kobayashi, A Higashitani

    INTERNATIONAL JOURNAL OF RADIATION BIOLOGY　82　(1)　31-38　2006/01

    DOI： 10.1080/09553000600577821 　

    ISSN： 0955-3002

  90. Proteomic analysis of Arabidopsis root gravitropism Peer-reviewed

    Kamada M, Atsushi Higashitani, A, Ishioka N

    Biological Science in Space　19　(3)　148-154　2005/12

    DOI： 10.2187/bss.19.148 　

  91. Perturbation of oocyte meiotic division by hypergravity in Caenorhabditis elegans Peer-reviewed

    Sasagawa Y. Saito, Y. Shimizu M. Takahashi, HIGASHITANI Atsushi

    Biological Science in Space　19　(3)　155-162　2005/12

    DOI： 10.2187/bss.19.155 　

  92. Checkpoint and physiological apoptosis in germ cells proceeds normally in spaceflown Caenorhabditis elegans Peer-reviewed

    A Higashitani, A Higashibata, Y Sasagawa, T Sugimoto, Y Miyazawa, NJ Szewcyk, M Viso, G Gasset, B Eche, K Fukui, T Shimazu, N Fujimoto, K Kuriyama, N Ishioka

    APOPTOSIS　10　(5)　949-954　2005/10

    DOI： 10.1007/s10495-005-1323-3 　

    ISSN： 1360-8185

  93. High-temperature induction of male sterility during barley (Hordeum vulgare L.) anther development is mediated by transcriptional inhibition Peer-reviewed

    M Abiko, K Akibayashi, T Sakata, M Kimura, M Kihara, K Itoh, E Asamizu, S Sato, H Takahashi, A Higashitani

    SEXUAL PLANT REPRODUCTION　18　(2)　91-100　2005/09

    DOI： 10.1007/s00497-005-0004-2 　

    ISSN： 0934-0882

  94. Caenorhabditis elegans Elongin BC complex is essential for cell proliferation and chromosome condensation and segregation during mitosis and meiotic division II Peer-reviewed

    Y Sasagawa, K Kikuchi, K Dazai, A Higashitani

    CHROMOSOME RESEARCH　13　(4)　357-375　2005

    DOI： 10.1007/s10577-005-2687-5 　

    ISSN： 0967-3849

    eISSN： 1573-6849

  95. Drosophila Nedd4 regulates endocytosis of notch and suppresses its ligand-independent activation Peer-reviewed

    T Sakata, H Sakaguchi, L Tsuda, A Higashitani, T Aigaki, K Matsuno, S Hayashi

    CURRENT BIOLOGY　14　(24)　2228-2236　2004/12

    DOI： 10.1016/j.cub.2004.12.028 　

    ISSN： 0960-9822

  96. Molecular characterization of four novel anther-specific genes in the model legume, Lotus japonicus. Peer-reviewed

    Hakozaki, H, Endo, M, Masuko, H, Park, J.-I, Ito, H, Uchida, M, Kamada, M, Takahashi, H, Higashitani, A, Watanabe, M

    Genes Genet. Syst.　79　307-310　2004/10

    DOI： 10.1266/ggs.79.307 　

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    ISI 被引用数：4

  97. Cloning and expression pattern of a novel microspore-specific gene encoding hypersensitive-induced response protein (LjHIR1) from the model legume, Lotus japonicus Peer-reviewed

    Hirokazu Hakozaki, Makoto Endo, Hiromi Masuko, Jong-In Park, Hitoshi Ito, Masanori Uchida, Motoshi Kamada, Hideyuki Takahashi, Atsushi Higashitani, Masao Watanabe

    Genes and Genetic Systems　79　(5)　307-310　2004/10

    DOI： 10.1266/ggs.79.307 　

    ISSN： 1341-7568

  98. Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray Peer-reviewed

    Makoto Endo, Tohru Tsuchiya, Hiroshi Saito, Hitoshi Matsubara, Hirokazu Hakozaki, Hiromi Masuko, Motoshi Kamada, Atsushi Higashitani, Hideyuki Takahashi, Hiroo Fukuda, Taku Demura, Masao Watanabe

    Genes and Genetic Systems　79　(4)　213-226　2004/08

    DOI： 10.1266/ggs.79.213 　

    ISSN： 1341-7568

  99. Gravity-induced modification of auxin transport and distribution for peg formation in cucumber seedlings: possible roles for CS-AUX1 and CS-PIN1 Peer-reviewed

    M Kamada, S Yamasaki, N Fujii, A Higashitani, H Takahashi

    PLANTA　218　(1)　15-26　2003/11

    DOI： 10.1007/s00425-003-1072-x 　

    ISSN： 0032-0935

  100. Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis Peer-reviewed

    Y Sasagawa, T Urano, Y Kohara, H Takahashi, A Higashitani

    GENES TO CELLS　8　(11)　857-872　2003/11

    DOI： 10.1046/j.1365-2443.2003.00682.x 　

    ISSN： 1356-9597

  101. Effects of gravity on early embryogenesis [correction of embryogeneis] in Caenorhabditis elegans. Peer-reviewed

    Sasagawa Y, Saito Y, Shimizu M, Takahashi H, Ishioka N, Fukui K, Yamashita M, Higashitani A

    Uchu Seibutsu Kagaku　17　(3)　217-218　2003/10

    ISSN： 0914-9201

  102. Efficient repair of DNA damage induced by heavy ion particles in meiotic prophase I nuclei of Caenorhabditis elegans Peer-reviewed

    Takako Takanami, Yongzhao Zhang, Hidetoshi Aoki, Tomoko Abe, Shigeo Yoshida, Hideyuki Takahashi, Saburo Horiuchi, Atsushi Higashitani

    Journal of Radiation Research　44　(3)　271-276　2003/09

    DOI： 10.1269/jrr.44.271 　

    ISSN： 0449-3060

  103. Hydrotropism interacts with gravitropism by degrading amyloplasts in seedling roots of Arabidopsis and radish Peer-reviewed

    Nobuyuki Takahashi, Yutaka Yamazaki, Akie Kobayashi, Atsushi Higashitani, Hideyuki Takahashi

    Plant Physiology　132　(2)　805-810　2003/06/01

    DOI： 10.1104/pp.018853 　

    ISSN： 0032-0889

  104. Spiral growth and cell wall properties of the gibberellin-treated first internodes in the seedlings of a wheat cultivar tolerant to deep-sowing conditions Peer-reviewed

    Lei Chen, Atsushi Higashitani, Hiroshi Suge, Kazuyoshi Takeda, Hideyuki Takahashi

    Physiologia Plantarum　118　(1)　147-155　2003/05/01

    DOI： 10.1034/j.1399-3054.2003.00093.x 　

    ISSN： 0031-9317

  105. Morphogenesis, hydrotropism, and distribution of auxin signals in cucumber seedlings grown in microgravity Invited

    H Takahashi, M Kamada, Y Saito, A Kobayashi, A Higashitani, N Fujii

    PLANT BIOTECHNOLOGY 2002 AND BEYOND　359-362　2003

  106. Caenorhabditis elegans Ce-rdh-1/rad-51 functions after double-strand break formation of meiotic recombination Peer-reviewed

    Takako Takanami, Akiyuki Mori, Hideyuki Takahashi, Saburo Horiuchi, Atsushi Higashitani

    Chromosome Research　11　(2)　125-135　2003

    DOI： 10.1023/A:1022863814686 　

    ISSN： 0967-3849

  107. [Gravity-induced asymmetry of localization of auxin-carrier proteins (CS-AUX1 and CS-PIN1) for peg formation in cucumber seedlings]. Peer-reviewed

    Kamada M, Fujii N, Higashitani A, Takahashi H

    Uchu Seibutsu Kagaku　16　(3)　153-154　2002/11

    ISSN： 0914-9201

  108. Generation of 919 expressed sequence tags from immature flower buds and gene expression analysis using expressed sequence tags in the model plant Lotus japonicus Peer-reviewed

    M. Endo, H. Hakozaki, T. Kokubun, H. Masuko, Y. Takahata, T. Tsuchiya, A. Higashitani, S. Tabata, M. Watanabe

    Genes and Genetic Systems　77　(4)　277-282　2002/08

    DOI： 10.1266/ggs.77.277 　

    ISSN： 1341-7568

  109. Response of the first internodes of Hong Mang Mai wheat to ethylene, gibberellins and potassium. Peer-reviewed

    Nishizawa T, Chen L, Higashitani A, Takahashi H, Takeda K, Suge H

    Plant Production Science　5　(2)　93-100　2002/04

    Publisher: CROP SCIENCE SOCIETY OF JAPAN

    DOI： 10.1626/pps.5.93 　

    ISSN： 1343-943X

    More details Close

    Seedlings of Hong Mang Mai wheat (Triticum aestivum L.) emerge from much deeper in the soil than do other cultivars by elongating their first internode. We examined the roles of ethylene, gibberellins (GA) and potassium in the elongation of the first internode in wheat cultivars. Although ethylene stimulated the elongation of the first internode of Hong Mang Mai in the dark, the ethylene evolution and the expression level of ethylene-receptor-like gene of this cultivar were not greater than those of other cultivars. In Hong Mang Mai, gibberellin A3 (GA3) substantially stimulated the elongation of the first internode in the dark. Maximum elongation of the first internode was obtained by GA3 at the concentrations of 2.89 × 10-6 to 2.89 × l0-7 M. This cultivar responded markedly to gibberellins A1 and A3 by elongating the first internode in the dark. A mixture of gibberellins A4 and A7 (GA4+7) was not effective in inducing the elongation. Haruhikari, a cultivar having no GA -insensitive-reducing-height (Rht) genes, substantially responded to GA3, but other cultivars did not. The differences in the sensitivity to GA could account, at least in part, for the varietal differences in the elongation of the first internode in wheat. Takune-komugi, a cultivar whose first internode elongates in response to ethylene, did not respond to GA3. Thus, there are different mechanisms for the GA- and ethylene-stimulated elongation of the first internode in wheat. Also, it was suggested that potassium uptake from the soil might function together with endogenous GA in inducing the elongation of the first internode in Hong Mang Mai wheat.

  110. On-board centrifuge required for space experiments of plants Peer-reviewed

    Higashitani, A., Takahashi, H.

    Biological sciences in space = Uchū seibutsu kagaku　15　(3)　157-159　2001/10

    DOI： 10.2187/bss.15.157 　

    ISSN： 0914-9201

  111. Auxin distribution and transport in the transition zone (peg region) between hypocotyl and root of cucumber seedlings Peer-reviewed

    Kamada, M., Fujii, N., Higashitani, A., Takahashi, H.

    Biological sciences in space = Uchū seibutsu kagaku　15　(3)　244-245　2001/10

    ISSN： 0914-9201

  112. Development of endodermal cells and expression of SCARECROW gene in weeping mutant of morning glory (Pharbitis nil) Peer-reviewed

    Kamada, M., Hatakeda, Y., Higashitani, A., Suge, H., Tasaka, M., Takahashi, H.

    Biological sciences in space = Uchū seibutsu kagaku　15　(3)　248-249　2001/10

    ISSN： 0914-9201

  113. Analysis of circumnutation in the seedlings (correction of seedings) of agravitropic mutants Peer-reviewed

    Hatakeda, Y., Takahashi, N., Kamada, M., Fujii, N., Higashitani, A., Suge, H., Fukaki, H., Tasaka, M., Goto, N., Takahashi, H.

    Biological sciences in space = Uchū seibutsu kagaku　15　(3)　250-251　2001/10

    ISSN： 0914-9201

  114. Repair systems for DNA damage by heavy-ion irradiation in meiotic cells Peer-reviewed

    Takanami, T., Takahashi, H., Abe, T., Yoshida, S., Higashitani, A.

    Biological sciences in space = Uchū seibutsu kagaku　15　(3)　274-275　2001/10

    ISSN： 0914-9201

  115. A variety of wheat tolerant to deep-seeding conditions: Elongation of the first internode depends on the response to gibberellin and potassium Peer-reviewed

    L. Chen, T. Nishizawa, A. Higashitani, H. Suge, Y. Wakui, K. Takeda, H. Takahashi

    Plant, Cell and Environment　24　(4)　469-476　2001

    DOI： 10.1046/j.1365-3040.2001.00688.x 　

    ISSN： 0140-7791

  116. Effects of high temperature on the development of pollen mother cells and microspores in barley Hordeum vulgare L. Peer-reviewed

    T Sakata, H Takahashi, Nishiyama, I, A Higashitani

    JOURNAL OF PLANT RESEARCH　113　(1112)　395-402　2000/12

    DOI： 10.1007/PL00013947 　

    ISSN： 0918-9440

  117. Caenorhabditis elegans Chk2-like gene is essential for meiosis but dispensable for DNA repair Peer-reviewed

    A Higashitani, H Aoki, A Mori, Y Sasagawa, T Takanami, H Takahashi

    FEBS LETTERS　485　(1)　35-39　2000/11

    DOI： 10.1016/S0014-5793(00)02178-5 　

    ISSN： 0014-5793

  118. Hyper-resistance of meiotic cells to radiation due to a strong expression of a single recA-like gene in Caenorhabditis elegans Peer-reviewed

    T Takanami, A Mori, H Takahashi, A Higashitani

    NUCLEIC ACIDS RESEARCH　28　(21)　4232-4236　2000/11

    DOI： 10.1093/nar/28.21.4232 　

    ISSN： 0305-1048

  119. Hyper-resistance of meiotic cells to radiation due to a strong expression of a single recA-like gene in Caenorhabditis elegans Peer-reviewed

    T Takanami, A Mori, H Takahashi, A Higashitani

    NUCLEIC ACIDS RESEARCH　28　(21)　4232-4236　2000/11

    DOI： 10.1093/nar/28.21.4232 　

    ISSN： 0305-1048

  120. [The gravity-regulated formation of peg and auxin transport in cucumber seedlings]. Peer-reviewed

    Kamada M, Sakata T, Fujii N, Higashitani A, Takahashi H

    Uchu Seibutsu Kagaku　14　(3)　240-241　2000/10

    ISSN： 0914-9201

  121. Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans Peer-reviewed

    H Aoki, S Sato, T Takanami, T Ishihara, Katsura, I, H Takahashi, A Higashitani

    MOLECULAR AND GENERAL GENETICS　264　(1-2)　119-126　2000/09

    DOI： 10.1007/s004380000291 　

    ISSN： 0026-8925

  122. Analysis of expressed sequence tags of flower buds in Lotus japonicus Peer-reviewed

    M Endo, T Kokubun, Y Takahata, A Higashitani, S Tabata, M Watanabe

    DNA RESEARCH　7　(3)　213-216　2000/06

    DOI： 10.1093/dnares/7.3.213 　

    ISSN： 1340-2838

  123. Morphogenesis in cucumber seedlings is negatively controlled by gravity Peer-reviewed

    H Takahashi, M Kamada, Y Yamazaki, N Fujii, A Higashitani, S Aizawa, Yoshizaki, I, S Kamigaichi, C Mukai, T Shimazu, K Fukui

    PLANTA　210　(3)　515-518　2000/02

    DOI： 10.1007/s004250050039 　

    ISSN： 0032-0935

  124. Gravimorphogenesis of Cucurbitaceae plants. Development of peg cells and graviperception mechanism in cucumber seedlings. Peer-reviewed

    Takahashi Hideyuki, Mizuno Hidetoshi, Kaneko Yasuko, Murata Takashi, Kamigaichi Shigeki, Aizawa Sachiko, Yoshizaki Izumi, Shimazu Toru, Fukui Keiji, Fujii Nobuharu, Kamada Motoshi, Higashitani Atsushi, Yamazaki Yutaka, Kobayashi Akie, Takano Mamoru, Yamasaki Seiji, Sakata Tadashi

    Biological Sciences in Space　14　(2)　64-74　2000

    Publisher: Japanese Society for Biological Sciences in Space

    DOI： 10.2187/bss.14.64 　

    ISSN： 0914-9201

    More details Close

    We examined the effect of microgravity on the peg formation of cucumber seedlings for clarifying the mechanism of gravimorphogenesis in cucurbitaceous plants. The spaceflight experiments verified that gravity controls the formation of peg, hypocotyl hook and growth orientation of cucumber seedlings. Space-grown cucumber developed a peg on each side of the transition zone of the hypocotyl and root, indicating that on the ground peg formation is regulated negatively by gravity (Takahashi et al. 2000). It was found that the auxin-regulated gene, CS-IAA1, was strongly expressed in the transition zone where peg develops (Fujii et al. 2000). In the seedlings grown horizontally on the ground, CS-IAA1 transcripts were much abundant on the lower side of the transition zone, but no such differential expression of CS-IAA1 was observed in the space-grown cucumber (Kamada et al. 2000). These results imply that gravity plays a role in peg formation through auxin redistribution. By the negative control, peg formation on the upper side of the transition zone in the horizontally growing seedlings might be suppressed due to a reduction in auxin concentration. The threshold theory of auxin concentration accounted for the new concept, negative control of morphogenesis by gravity (Kamada et al. 2000). Anatomical studies have shown that there exists the target cells destined to be a peg and distinguishable at the early stage of the growth. Ultra-structural analysis suggested that endoplasmic reticulum develops well in the cells of the future peg. Furthermore, it was found that re-organization of cortical microtubules is required for the change in cell growth polarity in the process of peg formation. The spaceflight experiment with cucumber seedlings also suggested that in microgravity positive hydrotropic response of roots occurred without interference by gravitropic response (Takahashi et al. 1999b). Thus, this spaceflight experiment together with the ground-based studies has shown that cucumber seedling is an ideal for the study of gravimorphogenesis, hydrotropism and their interaction. Although peg formation is seen specifically in cucurbitaceous seedlings, it involves graviperception, auxin transport and redistribution and cytoskeletal modification for controlling cell growth polarity. This system could be a useful model for studying important current issues in plant biology.

  125. A spaceflight experiment for the study of gravimorphogenesis and hydrotropism in cucumber seedlings Peer-reviewed

    H Takahashi, H Mizuno, M Kamada, N Fujii, A Higashitani, S Kamigaichi, S Aizawa, C Mukai, T Shimazu, K Fukui, M Yamashita

    JOURNAL OF PLANT RESEARCH　112　(1108)　497-505　1999/12

    DOI： 10.1007/PL00013906 　

    ISSN： 0918-9440

  126. [Microtubule reorganization in response to gravistimulation during cucumber peg development]. Peer-reviewed

    Murata T, Kobayashi M, Fujii N, Higashitani A, Aizawa S, Kamigaichi S, Shimazu T, Fukui K, Takahashi H

    Uchu Seibutsu Kagaku　13　(3)　282-283　1999/09

    ISSN： 0914-9201

  127. Filamentous phage replication initiator protein gpII forms a covalent complex with the 5 ' end of the nick it introduced Peer-reviewed

    S Asano, A Higashitani, K Horiuchi

    NUCLEIC ACIDS RESEARCH　27　(8)　1882-1889　1999/04

    DOI： 10.1093/nar/27.8.1882 　

    ISSN： 0305-1048

  128. Endoxyloglucan transferase cDNA isolated from pea roots and its fluctuating expression in hydrotropically responding roots Peer-reviewed

    M Takano, N Fujii, A Higashitani, K Nishitani, T Hirasawa, H Takahashi

    PLANT AND CELL PHYSIOLOGY　40　(2)　135-142　1999/02

    ISSN： 0032-0781

  129. A role of cytoskeletal structure of cortical cells in the gravity-regulated formation of a peg in cucumber seedlings Peer-reviewed

    M Kobayashi, T Murata, N Fujii, M Yamashita, A Higashitani, H Takahashi

    LIFE SCIENCES: MICROGRAVITY RESEARCH II　24　(6)　771-773　1999

    ISSN： 0273-1177

  130. Agravitropic mutant for the study of hydrotropism in seedling roots Peer-reviewed

    H Takahashi, M Takano, N Fujii, A Higashitani, M Yamashita, T Hirasawa, K Nishitani

    LIFE SCIENCES: MICROGRAVITY RESEARCH I　23　(12)　2021-2028　1999

    ISSN： 0273-1177

  131. Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination Peer-reviewed

    Takako Takanami, Shusei Sato, Takeshi Ishihara, Isao Katsura, Hideyuki Takahashi, Atsushi Higashitani

    DNA Research　5　(6)　373-377　1998

    Publisher: Universal Academy Press Inc.

    DOI： 10.1093/dnares/5.6.373 　

    ISSN： 1340-2838

  132. Functional dissection of a cell-division inhibitor, SulA, of Escherichia coli and its negative regulation by Lon Peer-reviewed

    A Higashitani, Y Ishii, Y Kato, K Horiuchi

    MOLECULAR & GENERAL GENETICS　254　(4)　351-357　1997/04

    DOI： 10.1007/s004380050426 　

    ISSN： 0026-8925

  133. Recognition mechanisms of the minus-strand origin of phage f1 by Escherichia coli RNA polymerase Peer-reviewed

    N Higashitani, A Higashitani, ZW Guan, K Horiuchi

    GENES TO CELLS　1　(9)　829-841　1996/09

    ISSN： 1356-9597

  134. SOS INDUCTION IN ESCHERICHIA-COLI BY SINGLE-STRANDED-DNA OF MUTANT FILAMENTOUS PHAGE - MONITORING BY CLEAVAGE OF LEXA REPRESSOR Peer-reviewed

    N HIGASHITANI, A HIGASHITANI, K HORIUCHI

    JOURNAL OF BACTERIOLOGY　177　(12)　3610-3612　1995/06

    ISSN： 0021-9193

    eISSN： 1098-5530

  135. A CELL-DIVISION INHIBITOR SULA OF ESCHERICHIA-COLI DIRECTLY INTERACTS WITH FTSZ THROUGH GTP HYDROLYSIS Peer-reviewed

    A HIGASHITANI, N HIGASHITANI, K HORIUCHI

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　209　(1)　198-204　1995/04

    DOI： 10.1006/bbrc.1995.1489 　

    ISSN： 0006-291X

  136. DNA replication in filamentous bacteriophage Peer-reviewed

    Higashitani, A., Higashitani, N., Horiuchi, K.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　39　(13)　2189-2197　1994/10

    ISSN： 0039-9450

  137. A GENERAL AND FAST METHOD FOR MAPPING MUTATIONS ON THE ESCHERICHIA-COLI CHROMOSOME Peer-reviewed

    A HIGASHITANI, N HIGASHITANI, S YASUDA, K HORIUCHI

    NUCLEIC ACIDS RESEARCH　22　(12)　2426-2427　1994/06

    DOI： 10.1093/nar/22.12.2426 　

    ISSN： 0305-1048

  138. MULTIPLE DNA CONFORMATIONAL-CHANGES INDUCED BY AN INITIATOR PROTEIN PRECEDE THE NICKING REACTION IN A ROLLING CIRCLE REPLICATION ORIGIN Peer-reviewed

    A HIGASHITANI, D GREENSTEIN, H HIROKAWA, S ASANO, K HORIUCHI

    JOURNAL OF MOLECULAR BIOLOGY　237　(4)　388-400　1994/04

    DOI： 10.1006/jmbi.1994.1242 　

    ISSN： 0022-2836

  139. 大腸菌繊維状ファージのDNA複製開始機構 Peer-reviewed

    HIGASHITANI Atsushi

    蛋白質核酸酵素　39　(13)　2189-2197　1994

    Publisher:

    ISSN： 0039-9450

  140. OSMOREGULATION OF THE FATTY-ACID RECEPTOR GENE FADL IN ESCHERICHIA-COLI Peer-reviewed

    A HIGASHITANI, Y NISHIMURA, H HARA, H AIBA, T MIZUNO, K HORIUCHI

    MOLECULAR & GENERAL GENETICS　240　(3)　339-347　1993/09

    ISSN： 0026-8925

  141. NUCLEOTIDE-SEQUENCE OF THE PRIMER RNA FOR DNA-REPLICATION OF FILAMENTOUS BACTERIOPHAGES Peer-reviewed

    N HIGASHITANI, A HIGASHITANI, K HORIUCHI

    JOURNAL OF VIROLOGY　67　(4)　2175-2181　1993/04

    ISSN： 0022-538X

  142. Characterization of meiosis-specific strand transfer activities in Lily microsporocytes

    Higashitani N, Higashitani A, Hotta Y, Tabata S

    Bulletin of the Institute for Chemical Research, Kyoto Univ.　73　(3)　259-267　1993/04

  143. Filamentous bacteriophage of E. coli Peer-reviewed

    Horiuchi, K., Higashitani, A.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　37　(14 Suppl)　2589-2598　1992/10

    ISSN： 0039-9450

  144. A SINGLE AMINO-ACID SUBSTITUTION REDUCES THE SUPERHELICITY REQUIREMENT OF A REPLICATION INITIATOR PROTEIN Peer-reviewed

    A HIGASHITANI, D GREENSTEIN, K HORIUCHI

    NUCLEIC ACIDS RESEARCH　20　(11)　2685-2691　1992/06

    DOI： 10.1093/nar/20.11.2685 　

    ISSN： 0305-1048

  145. SOS INDUCTION IN ESCHERICHIA-COLI BY INFECTION WITH MUTANT FILAMENTOUS PHAGE THAT ARE DEFECTIVE IN INITIATION OF COMPLEMENTARY-STRAND DNA-SYNTHESIS Peer-reviewed

    N HIGASHITANI, A HIGASHITANI, A ROTH, K HORIUCHI

    JOURNAL OF BACTERIOLOGY　174　(5)　1612-1618　1992/03

    ISSN： 0021-9193

  146. 繊維状ファージの複契機構と蛋白質展示ベクターとしての応用 Peer-reviewed

    HIGASHITANI Atsushi

    蛋白質核酸酵素　37　(14)　2589-2598　1992

  147. CLONING, MAPPING, AND CHARACTERIZATION OF THE ESCHERICHIA-COLI PRC GENE, WHICH IS INVOLVED IN C-TERMINAL PROCESSING OF PENICILLIN-BINDING PROTEIN-3 Peer-reviewed

    H HARA, Y YAMAMOTO, A HIGASHITANI, H SUZUKI, Y NISHIMURA

    JOURNAL OF BACTERIOLOGY　173　(15)　4799-4813　1991/08

    ISSN： 0021-9193

    eISSN： 1098-5530

  148. D-LOOP FORMING ACTIVITY AND GENERAL RECOMBINATORY ACTIVITY IN EXTRACTS FROM SHOOT, FLOWER AND CALLUS OF VARIETIES OF RICE Peer-reviewed

    A HIGASHITANI, S TAMAMOTO, S TABATA, K OONO, Y HOTTA

    JAPANESE JOURNAL OF GENETICS　65　(2)　53-64　1990/04

    DOI： 10.1266/jjg.65.53 　

    ISSN： 0021-504X

  149. ATP-INDEPENDENT STRAND TRANSFER PROTEIN FROM MURINE SPERMATOCYTES, SPERMATIDS, AND SPERMATOZOA Peer-reviewed

    A HIGASHITANI, S TABATA, T OGAWA, M SHIBATA, Y HOTTA

    EXPERIMENTAL CELL RESEARCH　186　(2)　317-323　1990/02

    DOI： 10.1016/0014-4827(90)90311-W 　

    ISSN： 0014-4827

  150. PURIFICATION OF DNA LIGASES FROM MOUSE TESTIS AND THEIR BEHAVIOR DURING MEIOSIS Peer-reviewed

    A HIGASHITANI, S TABATA, H ENDO, Y HOTTA

    CELL STRUCTURE AND FUNCTION　15　(1)　67-72　1990/02

    DOI： 10.1247/csf.15.67 　

    ISSN： 0386-7196

    eISSN： 1347-3700

  151. PURIFICATION OF DNA LIGASES FROM MOUSE TESTIS AND THEIR BEHAVIOR DURING MEIOSIS Peer-reviewed

    A HIGASHITANI, S TABATA, H ENDO, Y HOTTA

    CELL STRUCTURE AND FUNCTION　15　(1)　67-72　1990/02

    DOI： 10.1247/csf.15.67 　

    ISSN： 0386-7196

    eISSN： 1347-3700

  152. Erratum: D-loop forming activity and general recombinatory activity in extracts from shoot, flower and callus of varieties of rice (pp. 53-64)

    Higashitani, A., Tamamoto, S., Tabata, S., Oono, K., Hotta, Y.

    Japanese Journal of Genetics　65　(3)　153-153　1990

    ISSN： 0021-504X

  153. PLANT SAPONINS CAN AFFECT DNA RECOMBINATION IN CULTURED MAMMALIAN-CELLS Peer-reviewed

    A HIGASHITANI, S TABATA, T HAYASHI, Y HOTTA

    CELL STRUCTURE AND FUNCTION　14　(5)　617-624　1989/10

    DOI： 10.1247/csf.14.617 　

    ISSN： 0386-7196

    eISSN： 1347-3700

  154. Controls in meiosis Peer-reviewed

    Hotta, Y., Higashitani, A., Tabata, S.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　34　(9)　1082-1096　1989/07

    ISSN： 0039-9450

  155. CONSTRUCTION OF AN ORDERED COSMID COLLECTION OF THE ESCHERICHIA-COLI K-12 W3110 CHROMOSOME Peer-reviewed

    S TABATA, A HIGASHITANI, M TAKANAMI, K AKIYAMA, Y KOHARA, Y NISHIMURA, A NISHIMURA, S YASUDA, Y HIROTA

    JOURNAL OF BACTERIOLOGY　171　(2)　1214-1218　1989/02

    ISSN： 0021-9193

    eISSN： 1098-5530

  156. Molecular Biology of Meiosis Peer-reviewed

    HIGASHITANI Atsushi

    細胞工学　8　(10)　1989

  157. EFFECT OF INOCULATION WITH KLEBSIELLA-OXYTOCA AND ENTEROBACTER-CLOACAE ON DINITROGEN FIXATION BY RICE BACTERIA ASSOCIATIONS Peer-reviewed

    T FUJII, YD HUANG, A HIGASHITANI, Y NISHIMURA, S IYAMA, Y HIROTA, T YONEYAMA, RA DIXON

    PLANT AND SOIL　103　(2)　221-226　1987

    ISSN： 0032-079X

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    梅原真澄, 橋爪藤子, 東谷篤志, 東端晃

    日本宇宙航空環境医学会大会プログラム・予稿集　62nd　132　2016

  9. 高温ならびに低温障害と植物ホルモン─ 花粉形成がこれらストレスになぜ脆弱か ─

    東谷篤志

    Regulation of Plant Growth & Development　50　(2)　162-165　2015/12/21

    Publisher: The Japanese Society for Chemical Regulation of Plants

    DOI： 10.18978/jscrp.50.2_162 　

    ISSN： 1346-5406

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    <p>Studies on high-temperature injury and cool-weather damage in crops, wheat, barley and rice have a long history, and have identified physiological responses including abnormal abortion of developing pollen followed by male sterility. However, a full understanding of the molecular mechanism has not been achieved. We here show that endogenous auxin levels specifically decreased in the developing anthers of barley and Arabidopsis under high temperature conditions. Similarly, endogenous gibberellin levels significantly reduced in the developing anthers of rice by decreasing temperatures. In addition, the temperature fluctuations repressed each gene expression of auxin biosynthesis genes, YUCCA yuc-2 and yuc-6, and gibberellin biosynthesis genes, GA20ox3 and GA3ox1. These set of genes are dominantly expressed in the developing anthers indicating that at least auxin and gibberellin are synthesized in the anthers. It may lead to susceptibility to male sterility in temperature stress.</p>

  10. Caenorhabditis elegans displays different adaptations to single vs. multigenerational growth during spaceflight

    Tim Etheridge, Akira Higashibata, Nathaniel Szewczyk, Atsushi Higashitani

    FASEB JOURNAL　29　2015/04

    ISSN： 0892-6638

    eISSN： 1530-6860

  11. Development of the Experimental Plant Culture System for Clarifying Plant Responses to Space Environment

    Kitaya Yoshiaki, Higashitani Atsushi, Karahara Ichiro, Takahashi Hideyuki, Hoson Takayuki, Hirai Hiroaki, Yano Sachiko

    (29)　27-28　2015/01

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS)

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    Space Utilization Research (January 24-25, 2015. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS)), Sagamihara, Kanagawa Japan

  12. Low-Temperature Injury to Rice Pollen Development Occurs by Reductions of Bioactive Gibberellins

    ODA Susumu, SAKATA Tadashi, TSUNAGA Yuta, KAWAGISHI-KOBAYASHI Makiko, AYA Koichiro, SAEKI Kenichi, ENDO Takashi, NAGANO Kuniaki, SAKAKIBARA Hitoshi, WATANABE Masao, MATSUOKA Makoto, HIGASHITANI Atsushi

    日本植物生理学会年会要旨集　55th　2014

  13. Expression analysis of QTL candidate genes for a cool-temperature tolerance in rice

    SHOMURA Hikaru, ODA Susumu, TOMODA Aya, NAGANO Kuniaki, ENDO Takashi, SAEKI Kenichi, SATOU Tadashi, SATOU Shusei, HIGASHITANI Atsushi

    日本植物生理学会年会要旨集　55th　2014

  14. イネ低温障害の耐性に関わる第7染色体遺伝子座の探索

    友田文, 小田晋, 正村光, 古井瑛恵, 佐伯研一, 遠藤貴司, 永野邦明, 佐藤修正, 東谷篤志

    日本分子生物学会年会プログラム・要旨集(Web)　36th　2013

  15. 微小重力環境下におけるRNA干渉の有効性および筋萎縮とエネルギー代謝変化との関係

    東端晃, 橋爪藤子, 東谷なほ子, 根本華奈子, 森ちひろ, 原田俊介, 杉本朋子, ETHERIDGE Timothy, SZEWCZYK Nathaniel, 東谷篤志

    日本分子生物学会年会プログラム・要旨集(Web)　36th　3P-0045 (WEB ONLY)　2013

  16. 宇宙環境に対する植物反応解明のための実験系構築―宇宙での実験植物育成のための光照射方法の検討

    北宅善昭, 平井宏昭, 高橋秀幸, 山下雅道, 東谷篤志, 後藤英司, 齋藤高弘, 谷晃, 土屋広司, 多胡靖宏, 田山一郎, 神阪盛一郎, 保尊隆享, 高沖宗夫, 矢野幸子, 鎌田源司

    宇宙利用シンポジウム　28th　217-219　2012/03

  17. 2011年度生物科学系スモールペイロード宇宙実験研究チーム活動報告

    東端晃, 夏井坂誠, 福井啓二, 高橋秀幸, 東谷篤志, 馬嶋秀行, 曽我部正博, 高橋昭久, 二川健, 永瀬睦, 嶋津徹, 村瀬浩史, 行徳淳一郎, GUSEV Oleg, 山崎丘, 石岡憲昭

    宇宙利用シンポジウム　28th　185-186　2012/03

  18. 宇宙環境における線虫の老化―WG平成23年度活動報告―

    本田陽子, 東端晃, 石岡憲昭, 東谷篤志, 福井啓二, 田中雅嗣, 本田修二

    宇宙利用シンポジウム　28th　183-184　2012/03

  19. Effects of high- or cool-temperatures on microsporogenesis in plants

    Atsushi Higashitani

    GENES & GENETIC SYSTEMS　86　(6)　379-379　2011/12

    ISSN： 1341-7568

    eISSN： 1880-5779

  20. Molecular Mechanisms of Male Sterility Induced by High Temperatures

    KAWAGISHI KOBAYASHI Makiko, HIGASHITANI Atsushi

    80　304-305　2011/08/30

    ISSN： 0011-1848

  21. 廃用性筋萎縮の発生機序~オミクス技術を用いた無重力応答因子の探索~

    河野尚平, 近藤茂忠, 原田晃子, 平坂勝也, 真板綾子, 奥村裕司, 中尾玲子, 東端晃, 東谷篤志, 二川健

    日本栄養・食糧学会大会講演要旨集　65th　210　2011/04/25

  22. Toward Future Space Experiments for Life Sciences

    HOSON Takayuki, TAKAHASHI Akihisa, NIKAWA Takeshi, FUKUI Keiji, OGAWA Shiho, HIGASHITANI Atsushi

    Biological sciences in space　25　(1)　21-24　2011/04/01

    Publisher: 日本宇宙生物科学会

    ISSN： 0914-9201

  23. 宇宙環境に対する植物反応解明のための実験系構築―微小重力が植物茎内の蒸散流に及ぼす影響

    北宅善昭, 平井宏昭, 高橋秀幸, 山下雅道, 東谷篤志, 後藤英司, 齋藤高弘, 谷晃, 土屋広司, 多胡靖宏, 田山一郎, 神阪盛一郎, 保尊隆享, 高沖宗夫, 矢野幸子, 鎌田源司

    宇宙利用シンポジウム　27th　(27)　166-167　2011/03

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)

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    The Twenty-seventh Space Utilization Symposium (January 24-25, 2011. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)), Sagamihara, Kanagawa JapanA fundamental study was conducted to develop the experimental system to investigate effects of space environment on vegetative and reproductive growth of plants in their life cycles. Sap flow in plant stems plays an important role to transport water and nutrients internally from roots to leaves. In this study, the sap flow in tomato stems was assessed using a simple heat flow method at 0.01, 1.0 and 2.0 g for 20 seconds each during parabolic airplane flights in order to clarify the effect of microgravity on the sap flow in stems. Heat generated with a small heater installed in the stem was transferred upstream and downstream by conduction and upstream by convection with the sap flow through xylems of the vascular tissue. Thermal images of stem surfaces near heated points were captured using infrared thermography and the internal heat convection corresponding to the sap flow was analyzed. In results, the sap flow in stems at 0.01 g was shown to promote under a stirred air condition at a wind speed of 0.5 m s(exp -1) compared with that at 2.0 g. The promoted sap flow under the stirred air condition would be caused by removal of negative gravity effects for upward water flow under the microgravity conditions. The forced air movement is important to grow healthy plants under microgravity condition in space.Number of authors: 16Physical characteristics: Original contains color illustrations

  24. 「線虫C.elegansの宇宙環境におけるRNA干渉とタンパク質リン酸化」(CERISE)のISS宇宙実験速報

    東端晃, 橋爪藤子, 杉本朋子, 森ちひろ, 根本華奈子, 東谷なほ子, SZEWCZYK Nathaniel, 石岡憲昭, 福井啓二, 東谷篤志

    宇宙利用シンポジウム　27th　197　2011/03

  25. 宇宙環境における線虫の老化―WG平成22年度活動報告

    本田陽子, 東端晃, 石岡憲昭, 東谷篤志, 福井啓二, 田中雅嗣, 本田修二

    宇宙利用シンポジウム　27th　195-196　2011/03

  26. 2010年度生物科学系スモールペイロード宇宙実験研究班WG活動報告

    東端晃, 夏井坂誠, 福井啓二, 高橋秀幸, 東谷篤志, 馬嶋秀行, 曽我部正博, 高橋昭久, 二川健, 永瀬睦, 嶋津徹, 村瀬浩史, 行徳淳一郎, GUSEV Oleg, 山崎丘, 石岡憲昭

    宇宙利用シンポジウム　27th　219-220　2011/03

  27. 植物の生殖成長にみられる低温ならびに高温障害の分子メカニズムの解明

    阪田忠, 津長雄太, 八木橋奈央, 押野健, 三浦慎也, 苫米地真理, 藤岡智明, 佐藤修正, 佐藤修正, 川岸万紀子, 松岡信, 宮沢豊, 高橋秀幸, 渡辺正夫, 東谷篤志

    日本植物生理学会年会要旨集　52nd　2011

  28. 運動負荷が線虫の遺伝子およびタンパク質発現に与える影響について

    橋爪藤子, 東谷なほ子, 福井啓二, 石岡憲昭, 東谷篤志, 東端晃

    日本分子生物学会年会プログラム・要旨集(Web)　34th　2P-0141 (WEB ONLY)　2011

  29. 宇宙環境に対する植物反応解明のための実験系構築―微小重力下での植物茎内の水の流れ

    北宅善昭, 平井宏昭, 高橋秀幸, 山下雅道, 東谷篤志, 後藤英司, 齋藤高弘, 谷晃, 土屋広司, 多胡靖宏, 田山一郎, 神阪盛一郎, 保尊隆享, 高沖宗夫, 矢野幸子, 鎌田源司

    宇宙利用シンポジウム　26th　171-172　2010/03

  30. 平成21年度生物科学系スモールペイロード宇宙実験研究班WG活動報告

    東端晃, 夏井坂誠, 福井啓二, 高橋秀幸, 東谷篤志, 馬嶋秀行, 曽我部正博, 高橋昭久, 二川健, 永瀬睦, 嶋津徹, 村瀬浩史, 行徳淳一郎, GUSEV Oleg, 山崎丘, 石岡憲昭

    宇宙利用シンポジウム　26th　201-202　2010/03

  31. イネ低温障害時にみられる植物ホルモン関連遺伝子群の発現変動の解析

    津長雄太, 阪田忠, 藤岡智明, 増子潤実, 諏訪部圭太, 諏訪部圭太, 永野邦明, 川岸万紀子, 渡辺正夫, 東谷篤志

    日本植物生理学会年会要旨集　51st　2010

  32. 17. Auxin reduction is a primary cause of high temperature injury to male reproductive development

    Sakata Tadashi, Miura Shinya, Oshino Takeshi, Tomabechi Mari, Tsunaga Yuta, Yano Kentaro, Miyazawa Yutaka, Takahashi Hideyuki, Watanabe Masao, Higashitani Atsushi

    (44)　31-31　2009/10/06

    Publisher: The Japanese Society for Chemical Regulation of Plants

    ISSN： 0919-1887

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    Plant high-temperature (HT) injury has become a serious problem associated with global warming. The early phase of anther development is especially susceptible to HT injury in wheat, barley and other crops. Here, we have found that HT conditions showed a high correlation coefficiency with transcriptional repression of replication-related and auxin-induced genes. The application of auxin to barley completely reversed HT-induced male sterility. Similar reductions in auxin levels and reversibility by exogenous auxin application occurred in the developing anther cells of HT exposed Arabidopsis. In addition, YUC2 and -6 gene transcripts decreased by HT treatment in developing anther. These data suggest that tissue-specific auxin reduction causes comprehensive transcriptional inhibition including anther specific auxin biosynthesis genes. It is a primary cause of HT injury, which leads to the abortion of pollen development, and moreover suggests a novel use for auxin treatment to sustain steady yields of crops under conditions of climate change.

  33. 宇宙環境に対する植物反応解明のための実験系構築―微小重力下での植物葉からの蒸散の動態および植物育成チャンバー内の環境変動の解明

    北宅善昭, 高橋秀幸, 山下雅道, 東谷篤志, 平井宏昭, 後藤英司, 齋藤高弘, 谷晃, 土屋広司, 多胡靖宏, 田山一郎, 神阪盛一郎, 保尊隆享, 高沖宗夫, 矢野幸子, 鎌田源司

    宇宙利用シンポジウム　25th　(25)　30-31　2009/03

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)

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    The Twenty-fifth Space Utilization Symposium (January 14-15, 2009: ISAS/JAXA Sagamihara, Japan)A fundamental study was conducted to develop the experimental system to investigate effects of space environment on vegetative and reproductive growth of plants in their life cycles. In this study, the transpiration rate of plant leaves were evaluated at 0.01, 1.0 and 2.0 g for 20 sec each during parabolic airplane flights in order to clarify effects of microgravity on the water vapor exchange between plants and the ambient air. The transpiration rate of leaves was suppressed by increasing the water vapor transfer resistance due to restricted free air convection under microgravity conditions. Planning for evaluating performance of the water recycle system in a plant growth chamber under microgravity conditions is also described.

  34. モデル生物線虫を利用した宇宙実験とバイオドシメータ

    東谷篤志, 森ちひろ, 和田成一, 坂下哲哉, 小林泰彦, 杉本朋子, 山崎丘, 福井啓二, 東端晃, 石岡憲昭

    宇宙利用シンポジウム　25th　145-146　2009/03

  35. Ataxia telangiectasiaファミリーによるミトコンドリアDNAの維持機構

    森 ちひろ, 東谷 篤志

    放射線生物研究　43　(3)　272-283　2008/09

    Publisher: 放射線生物研究会

    ISSN： 0441-747X

  36. Male sterility accompanied with abnormal anther development in plants - genes and environmental stresses with special reference to high temperature injury.

    Sakata T, Higashitani A

    International Journal of Plant Developmental Biology　2　(1)　42-51　2008/05

  37. 宇宙環境に対する植物反応解明のための実験系構築―植物生殖成長に対する微小重力の影響

    北宅善昭, 高橋秀幸, 山下雅道, 東谷篤志, 後藤英司, 齋藤高弘, 谷晃, 土屋広司, 多胡靖宏, 田山一郎, 神阪盛一郎, 保尊隆享, 高沖宗夫, 矢野幸子, 鎌田源司

    宇宙利用シンポジウム　24th　304-305　2008/03

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)

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    A fundamental study was conducted to develop the experimental system to investigate the effects of space environment on vegetative and reproductive growth of plants in their life cycles. In this study, the thermal situation of the plant reproductive organs as affected by gravity levels was determined. Effects of gravity levels of 0.01, 1.0 and 2.0 g for 20 seconds each during parabolic airplane flights was investigated in order to make an estimation of temperature increases in the reproductive organs in closed plant growth facilities under microgravity in space.

  38. 宇宙フライトにより発現変動するゲノム遺伝子の網羅的な機能解析とデータベース構築

    東谷篤志, 東端晃

    宇宙利用シンポジウム　24th　240-241　2008/03

  39. 平成19年度生物科学系スモールペイロード宇宙実験研究班WG活動報告

    東端晃, 福井啓二, 高橋秀幸, 東谷篤志, 馬嶋秀行, 曽我部正博, 高橋昭久, 二川健, 最上善広, 永瀬睦, 嶋津徹, 藤本信義, 山崎丘, 石岡憲昭

    宇宙利用シンポジウム　24th　337-338　2008/03

  40. 高温によるイネ雄性不稔における遺伝子発現制御

    川岸万紀子, 遠藤誠, 佐藤繭子, 豊岡公徳, 大島正弘, 東谷篤志, 渡辺正夫

    生化学　2008

    ISSN： 0037-1017

  41. 宇宙環境に対する植物反応解明のための実験系構築―植物生殖器官の温度の実態およびその制御

    北宅善昭, 高橋秀幸, 山下雅道, 後藤英司, 齋藤高弘, 谷晃, 土屋広司, 多胡靖宏, 田山一郎, 神阪盛一郎, 保尊隆享, 東谷篤志, 高沖宗夫, 矢野幸子, 鎌田源司

    宇宙利用シンポジウム　23rd　385-388　2007/03

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)

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    Fundamental studies have been initiated to develop the experimental system to investigate the effects of space environment on vegetative and reproductive growth of plants in their life cycles. In this study, thermal situation of plant reproductive organs as affected by illumination and air movement was accessed on the earth. Thermal images of reproductive organs of rice and strawberry were captured using infrared thermography at air temperatures of 10 to 11 C. Temperatures of petals, stigmata and anthers of strawberry increased by 24, 22 and 14 C, respectively, after lighting at an irradiance of 40 W/sq m under incandescent lamps. Temperatures of reproductive organs and leaves of strawberry were significantly higher than those of rice. The temperatures in petals, stigmata, anthers and leaves of strawberry decreased by 12.8, 11.9, 13.1 and 14.1 C, respectively, when the air velocity increased from 0.1 to 1.0 m/s. Air movement was essential to reduce the temperatures of plant reproductive organs in closed plant growth facilities.

  42. 線虫(C.elegans)を用いる回収衛星宇宙実験研究班WG活動報告

    石岡憲昭, 石原昭彦, 宇佐美真一, 高橋昭久, 夏井坂誠, 二川健, 東谷篤志, 東端晃, 藤本信義, 馬嶋秀行, 保田浩志, 山崎丘

    宇宙利用シンポジウム　23rd　340-341　2007/03

  43. モデル生物Cエレガンスを用いた宇宙実験

    東谷篤志, 東端晃, 森ちひろ, 鈴木蓉子, 東谷なほ子, 山崎丘, 福井啓二, 石岡憲昭

    宇宙利用シンポジウム　23rd　339　2007/03

  44. 宇宙ストレス生物学研究班WG活動報告

    宇佐美真一, 浅島誠, 石岡憲昭, 石原昭彦, 泉龍太郎, 大石浩隆, 大森克徳, 鎌田源司, 黒谷明美, 芝大, 鈴木ひろみ, 曾我部正博, 高橋昭久, 二川健, 東谷篤志, 東端晃, 藤本信義, 馬嶋秀行, 矢野幸子, 山崎丘

    宇宙利用シンポジウム　23rd　349-350　2007/03

  45. 平成18年度生物科学系スモールペイロード宇宙実験研究班WG活動報告

    東端晃, 福井啓二, 高橋秀幸, 東谷篤志, 馬嶋秀行, 曽我部正博, 高橋昭久, 二川健, 最上善広, 永瀬睦, 嶋津徹, 藤本信義, 山崎丘, 石岡憲昭

    宇宙利用シンポジウム　23rd　342-343　2007/03

  46. 宇宙環境を利用するライフサイエンス実験 : 宇宙へヒトが活躍する場の拡大に向けて

    大西 武雄, 馬嶋 秀行, 高橋 秀幸, 東谷 篤志, 石岡 憲昭

    Isotope news　(633)　2-12　2007/01/01

    ISSN： 0285-5518

  47. Expression analysis of rice anther specific genes under high temperature stress condition

    Makoto Endo, Tohru Tsuchiya, Masahiro Oshima, Atsushi Higashitani, Masao Watanabe, Makiko Kawagishi

    PLANT AND CELL PHYSIOLOGY　48　S152-S152　2007

    ISSN： 0032-0781

  48. Analysis of entire gene expression during high-temperature injury to anther development in barley plants

    Takeshi Oshino, Mafumi Abiko, Rumiko Saito, Eiichiro Ichiishi, Makoto Endo, Makiko Kawagishi-Kobayashi, Atsushi Higashitani

    PLANT AND CELL PHYSIOLOGY　48　S185-S185　2007

    ISSN： 0032-0781

  49. A novel gene responsible for hydrotropism in Arabidopsis roots

    Akie Kobayashi, Akiko Takahashi, Yoko Kakimoto, Yutaka Miyazawa, Nobuharu Fujii, Atsushi Higashitani, Hideyuki Takahashi

    PLANT AND CELL PHYSIOLOGY　48　S144-S144　2007

    ISSN： 0032-0781

  50. 線虫の放射線応答:重イオンマイクロビームを用いた研究結果を中心に

    森 ちひろ, 東谷 篤志

    放射線生物研究　41　(4)　399-408　2006/12

    Publisher: 放射線生物研究会

    ISSN： 0441-747X

  51. Proteomics approach to gravitropism in Arabidopisis thaliana

    KAMADA Motoshi, HIGASHITANI Atsushi, ISHIOKA Noriaki

    23　(4)　334-334　2006/11/30

    ISSN： 0915-3616

  52. Decreased expression of myogenic transcription factors and myosin heavy chains in Caenorhabditis elegans muscles developed during spaceflight (vol 209, pg 3209, 2006)

    A. Higashibata, N. J. Szewczyk, C. A. Conley, M. Imamizo-Sato, A. Higashitani, N. Ishioka

    JOURNAL OF EXPERIMENTAL BIOLOGY　209　(18)　3695-3695　2006/09

    DOI： 10.1242/jeb.02504 　

    ISSN： 0022-0949

  53. Study of DNA damage response in germline cells of C. elegans

    HIGASHITANI ATSUSHI

    25　(1)　45-46　2006/03/31

    ISSN： 0912-3636

  54. RNA interference and protein phosphorylation in space environment using the nematode Caenorhabditis elegans

    東谷篤志, 東端晃, 石岡憲昭

    宇宙航空研究開発機構研究開発報告 JAXA-RR-　5　(05-033)　1-8　2006/03/10

    Publisher: Japan Aerospace Exploration Agency

    ISSN： 1349-1113

    More details Close

    It is important for human life in space to study the effects of environmental factors during spaceflight on a number of physiological phenomena. In the ICE-first experiment (International Caenorhabditis elegans Experiment-1, Mission DELTA, April 2004), we found that (1) both pachytene-checkpoint apoptosis and physiological apoptosis in germ cells occurred normally under spaceflight conditions and (2) certain genes related to signaling pathway of G-protein coupled receptor protein and to locomotory behavior tended to decrease in the spaceflown C. elegans. (3) In the hypergravity condition, we determined that the most susceptible aspect in the life cycle of this organism is the oocyte meiotic division for exclusion of polar bodies and the anterior-posterior polarization shortly after fertilization. (4) We also found several genes specifically responding to environmental stresses such as radiation by transcriptome analyses of entire genome using DNA microarray in several mutants and RNA interference (RNAi) animals. Since these phenomena and the identified genes are highly conserved in mammalian systems, the model experiments using this organism are useful to study the effects of space environment on human life. We are now attempting to study the RNAi effect, signal transduction, adaptation and evolution responding to space environment in future space experiments using the nematode C. elegans.

  55. 宇宙環境に対する植物反応解明のための実験系構築

    北宅善昭, 高橋秀幸, 山下雅道, 後藤英司, 斎藤高弘, 谷晃, 土屋広司, 多胡靖宏, 田山一郎, 神阪盛一郎, 保尊隆享, 東谷篤志, 高沖宗夫, 矢野幸子, 鎌田源司

    宇宙利用シンポジウム　22nd　(22)　305-306　2006/03

    Publisher: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)

    More details Close

    The Twenty-second Space Utilization Symposium (January 17-19, 2006: Science Council of Japan, Roppongi, Tokyo, Japan)In order to develop the experimental system for carrying out experiments with plants in space successfully, a working group was organized. The main objective is to clarify the effects of space environment on vegetative growth and reproductive growth of plants in their life cycles.Meeting sponsors: The Science Council of Japan, The Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (ISAS)(JAXA)

  56. モデル生物線虫を用いたICE‐first宇宙実験の成果と今後の展開について

    東谷篤志, 東端晃, 石岡憲昭

    宇宙利用シンポジウム　22nd　257-258　2006/03

  57. 線虫国際共同実験(ICE‐First)の概要および成果について

    東端晃, 香川弘昭, 本田修二, 東谷篤志, 石岡憲昭

    宇宙利用シンポジウム　22nd　254-256　2006/03

  58. High-temperature injury to barley anther development is mediated by transcriptional inhibition.

    M Abiko, K Akibayashi, H Takahashi, A Higashitani

    PLANT AND CELL PHYSIOLOGY　46　S132-S132　2005

    ISSN： 0032-0781

  59. High temperature injury to barley microsporogenesis and its proteomics analysis

    K Akibayashi, T Oshino, M Abiko, N Higashitani, A Higashitani

    PLANT AND CELL PHYSIOLOGY　46　S132-S132　2005

    ISSN： 0032-0781

  60. Role of gibberellin-induced cell division in first internode elongation of a wheat variety tolerant to deep-sowing

    M Araki, L Chen, Y Miyazawa, N Fujii, H Suge, K Takeda, A Higashitani, H Takahashi

    PLANT AND CELL PHYSIOLOGY　46　S211-S211　2005

    ISSN： 0032-0781

  61. High-temperature injury to early development of tapetum cells in barley Hordeum vulgare L.

    M Abiko, T Sakata, H Takahashi, E Asamizu, S Sato, S Tabata, M Kihara, K Ito, A Higashitani

    PLANT AND CELL PHYSIOLOGY　44　S135-S135　2003

    ISSN： 0032-0781

  62. Differences in endophytic colonization of nitrogen fixation bacteria Herbaspirillum sp. B501 in wild and cultivated rice.

    Abe T., Minamisawa K., Higashitani A., Ishii T., Sato T.

    Japanese Journal of Crop Science　71　(2)　70-71　2002/08/25

    Publisher: The Crop Science Society of Japan

    ISSN： 0011-1848

  63. Hydrotropic response of Arabidopsis and Raphanus roots accompanies a rapid reduction of starch-amyloplasts in collumela cells

    Y Yamazaki, N Takahashi, A Higashitani, H Takahashi

    PLANT AND CELL PHYSIOLOGY　43　S232-S232　2002

    ISSN： 0032-0781

  64. High-temperature injury and gene expression during development of barley pollen mother cells.

    M Abiko, T Sakata, H Takahashi, E Asamizu, S Sato, S Tabata, K Ito, A Higashitani

    PLANT AND CELL PHYSIOLOGY　43　S33-S33　2002

    ISSN： 0032-0781

  65. Gravity-induced modification of auxin transport and distribution for pea formation in cucumber seedlings

    M Kamada, N Fujii, A Higashitani, H Takahashi

    PLANT AND CELL PHYSIOLOGY　43　S59-S59　2002

    ISSN： 0032-0781

  66. 植物を用いた宇宙実験系におけるセントリフュージの必要性

    東谷 篤志, 高橋 秀幸

    宇宙生物科学 = Biological sciences in space　15　(3)　157-159　2001/11

    Publisher: 日本宇宙生物科学会

    ISSN： 0914-9201

  67. Morphogenesis in cucumber seedlings is negatively controlled by gravity

    TAKAHASHI Hideyuki, KAMADA Motoshi, YAMAZAKI Yutaka, FUJII Nobuharu, HIGASHITANI Atsushi, AIZAWA Sachiko, YOSHIZAKI Izumi, KAMIGAICHI Shigeki, MUKAI Chiaki, SHIMAZU Toru, FUKUI Keiji

    Biological sciences in space　15　s235-s238　2001/10

    Publisher: 日本宇宙生物科学会

    ISSN： 0914-9201

  68. ROLES OF POTASSIUM AND GIBBERELLIN FOR FIRST INTERNODE ELONGATION OF A WHEAT TOLERANT TO DEEP-SEEDING :

    CHEN Lei, HIGASHITANI Atsushi, SUGE Hiroshi, Takeda Kazuyoshi, TAKAHASHI Hideyuki

    Plant and cell physiology　42　s79　2001

    Publisher: Japanese Society of Plant Physiologists

    ISSN： 0032-0781

  69. Molecular mechanisms for reproductive development and repair systems for DNA damage by radiation in C. elegans.

    HIGASHITANI Athushi, TAKANAMI Takako, AOKI Hidetoshi, MORI Akiyuki, SASAGAWA Yohei, TAKAHASHI Hideyuki

    14　(3)　174-175　2000/10

    ISSN： 0914-9201

  70. Study for complete cereal plant life cycle in space. : Molecular analysis for the effect of high temperature on plant reproductive growth.

    SAKATA Tadashi, ABIKO Mafumi, HIGASHITANI Atsushi, TAKAHASHI Hideyuki

    14　(3)　168-169　2000/10

    ISSN： 0914-9201

  71. A survey reproductive organ specific genes of Lotus japonicus using cDNA microarray

    ENDO M., KOKUBUN T., MATSUBARA H., MASUKO H., TAKAHATA Y., TUTCHIYA T., HIGASHITANI A., TABATA T., WATANABE M.

    2　(2)　261-261　2000/09/25

    ISSN： 1344-7629

  72. 微小重力下における根の水分屈性

    高橋 秀幸, 水野 英俊, 鎌田 源司, 藤井 伸治, 東谷 篤志, 相澤 幸子, 上垣内 茂樹, 向井 千秋, 嶋津 徹

    根の研究 = Root research　9　(2)　92-92　2000/06/22

    ISSN： 0919-2182

  73. APPROACH FOR IDENTIFICATION OF THE ENTIRE GENE EXPRESSION DURING REPRODUCTIVE DEVELOPMENT OF BARLEY (Hordeum vulgare L.) :

    HIGASHITANI Atsushi, SAKATA Tadashi, TAKAHASHI Hideyuki

    Plant and cell physiology　41　s128　2000

    Publisher: Japanese Society of Plant Physiologists

    ISSN： 0032-0781

  74. GIBBERELLIN RESPONSE FOR FIRST INTERNODE ELONGATION OF A WHEAT TOLERANT TO DEEPSEEDING :

    CHEN Lei, HIGASHITANI Atsushi, KOMATSU Setsuko, TAKAHASHI Hideyuki

    Plant and cell physiology　41　s194　2000

    Publisher: Japanese Society of Plant Physiologists

    ISSN： 0032-0781

  75. Molecular mechanisms for sexual reproduction in terrestrial organisms and its relation to planetray environment: analysis of the barley sexual reproduction and an environmental stress

    SAKATA Tadashi, HIGASHITANI Atsushi, NISHIYAMA Iwao, TAKAHASHI Hideyuki

    13　(3)　286-287　1999/09

    ISSN： 0914-9201

  76. Negative control by gravity for the formation of peg in cucurbitaceous seedlings: Expession of the auixn-regulated genes in cucumber seedlings grown on the ground and under microgravity conditions

    KAMADA Motoshi, FUJII Nobuharu, HIGASHITANI Atsushi, AIZAWA Sachiko, KAMIGAICHI Shigeki, SHIMAZU Toru, FUKUI Keiji, TAKAHASHI Hideyuki

    宇宙生物科学 = Biological sciences in space　13　(3)　280-281　1999/09

    ISSN： 0914-9201

  77. STUDY OF HIGH TEMPERATURE EFFECTS AND CONSTRUCTION OF MOLECULAR MARKERS ON REPRODUCTIVEGROWTHIN BARLEYPLANT

    SAKATA Tadashi, TAKAHASHI Hideyuki, NISHIYAMA Iwao, HIGASHITANI Atsushi

    40　s26-s26　1999/03

    ISSN： 0032-0781

  78. DEVELOPMENTAL PATTERN OF PEG IN CUCUMBER SEEDLINGS AND ITS NEGATIVE CONTROL BY GRAVITY

    TAKAHASHI Hideyuki, FUJII Nobuharu, KAMADA Motoshi, TAKANO Mamoru, YAMAZAKI Yutaka, HIGASHITANI Atsushi, KANEKO Yasuko, KAMIGAICHI Shigeki, AIZAWA Sachiko, YOSHIZAKI Izumi, SHIMAZU Toru, FUKUI Keiji

    40　s144-s144　1999/03

    ISSN： 0032-0781

  79. Relationship between Varietal Differences in the Adaptability of Wheat Seedlings and Their Response to Ethylene

    NISHIZAWA Takeaki, SUGE Hiroshi, HIGASHITANI Atushi, TAKAHASHI Hideyuki

    Tohoku Journal of Crop Science　(41)　57-59　1998/12/24

    Publisher: The Crop Science Society of Japan

  80. Mechanism of the differential growth in hydrotropically responding pea roots

    TAKANO Mamoru, FUJII Nobuharu, HIGASHITANI Atushi, HIRASAWA Tadashi, NISHITANI Kazuhiko, TAKAHASHI Hideyuki

    7　(4)　126-126　1998/12/16

    ISSN： 0919-2182

  81. Characterization of a C. elegans recA-like gene Ce-rdh-1.

    TAKANAMI Takako, TAKAHASHI Hideyuki, SATO Shusei, ISHIHARA Takeshi, KATSURA Isao, HIGASHITANI Atsushi

    21　383-383　1998/12/01

  82. Effect of gibberellins on the elongation of first internode due to deep-seeding in wheat seedlings

    NISHIZAWA Takeaki, HIGASHITANI Atushi, SUGE Hiroshi, TAKAHASHI Hideyuki

    Japanese Journal of Crop Science　67　(2)　34-35　1998/10/08

    Publisher: The Crop Science Society of Japan

    ISSN： 0011-1848

  83. A ROLE OF CYTOSKELTAL STRUCTURE OF CORTICAL CELLS IN PEG FORMATION OF CUCUMBER SEEDLINGS

    KOBAYASHI Mayumi, MURATA Takashi, FUJII Nobuharu, HIGASHITANI Atsushi, TAKAHASHI Hideyuki

    39　S90-S90　1998/05

    ISSN： 0032-0781

  84. 微小重力下における根の姿勢制御関連遺伝子の発現

    高野 守, 藤井 伸治, 東谷 篤志, 高橋 秀幸, 西谷 和彦, 平沢 正, 山下 雅道

    宇宙生物科学 = Biological sciences in space　11　(3)　252-252　1997/09

    ISSN： 0914-9201

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Books and Other Publications 6

  1. 宇宙環境を利用するライフサイエンス実験‐宇宙へヒトが活躍する場の拡大に向けて‐

    大西武雄, 馬嶋秀行, 高橋秀幸, 東谷篤志, 石岡憲昭

    Isotope News　2007/01/07

  2. 放射線照射に対する生物応答：モデル生物Cエレガンスを用いたイオンマイクロビームの照射研究

    東谷篤志

    放射線と産業　2006/09/01

  3. 線虫の放射線応答：重イオンマイクロビームを用いた研究結果を中心に

    森ちひろ, 東谷篤志

    放射線生物研究　2006

  4. 減数分裂過程における放射線の影響

    高浪タカ子, 東谷篤志

    化学と生物 41: 138‐141　2003/10

  5. 減数分裂細胞の放射線抵抗性と遺伝子組換え酵素の発現.

    高浪タカ子, 堀内三郎, 東谷篤志

    放射線生物研究 38: 180-188　2003/06

  6. 朝倉植物生理学講座第5巻 環境応答

    高橋秀幸, 東谷篤志

    朝倉書店　2001/06

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Presentations 24

  1. Neuromuscular Signaling via TGF-β/DBL-1 Acts to Alter Body Physique in Response to Environmental Conditions International-presentation

    Shunsuke Harada, Toko Hashizume, Kanako Nemoto, Zhenhua Shao, Nahoko Higashitani, Timothy Etheridge, Nathaniel J Szewczyk, Keiji Fukui, Akira Higashibata, Atsushi Higashitani

    AMS2016(11th Asian Microgravity Symposium)　2016/10/25

  2. DAF-16 is required for recovering from heat injury to muscle cells. International-presentation

    Kenta Momma, Yohei Matsunaga, Takashi Homma, Hiroshi Qadota, Guy Benian, Atsushi Higashitani

    The 2016 C. elegans Topic Meeting: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs.　2016/07/21

  3. Neuromuscular Signaling via TGF-/DBL-1 Acts to Alter Body Physique in Response to Environmental Conditions. International-presentation

    Shunsuke Harada, Toko Hashizume, Kanako Nemoto, Zhenhua Shao, Nahoko Higashitani, Timothy Etheridge, Nathaniel J Szewczyk, Keiji Fukui, Akira Higashibata, Atsushi Higashitani

    The 2016 C. elegans Topic Meeting: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs.　2016/07/21

  4. The nematode C. elegans as a model organism for understanding the influence of microgravity International-presentation

    10th Asian Microgravity Symposium　2014/10/28

  5. Plant hormones and male sterility in cereal crops caused by temperature stress International-presentation

    Atsushi Higashitani, Tadashi Sakata, Takeshi Oshino, Kentaro Yano, Masao Watanabe

    International Botanical Congress 2011　2011/07/23

  6. RNA Interference and microRNA Expression in Space International-presentation

    28th International Symposium on Space Technology and Science.　2011/06/05

  7. 最先端の技術で微小重力の影響を狙いうち（線虫の実験）

    国際宇宙ステーション『きぼう』日本実験棟利用成果ミニシンポジウム　2011/02/28

  8. 小さなモデル生物を用いた宇宙実験

    市民公開講演会. 「ナノから宇宙へ、大きく広がる生命の科学」　2010/09/19

  9. 作物の高温. 不稔の生理と制御

    第5回 東北育種研究会　2010/08/26

  10. 植物ホルモンで作物の高温障害を防ぐ

    東谷篤志, 阪田忠, 渡辺正夫

    農林水産省農林水産技術会議：シンポジウム「ここまできた！お米の研究最前線」　2010/07/21

  11. Anther-specific auxin reduction is the primary cause of high temperature injury in Arabidopsis and barley plants International-presentation

    11th Asian and Oceanian Conference on transcription.　2010/07/01

  12. Auxins reverse male sterility caused by high temperatures International-presentation

    A. Higashitani, T. Sakata, T. Oshino, S. Miura, M. Tomabechi, Y. Tsunaga, N. Higashitani, Y. Miyazawa, H. Takahashi, M. Watanabe

    21th International conference on Arabidopsis research　2010/06/06

  13. Auxin reduction is a primary cause of high temperature injury to male reproductive development International-presentation

    Higashitani, Atsushi, Sakata, T, Miura, S, Oshino, T, Tomabechi, M, Tsunaga, Y, Yano, K, Miyazawa, Y, Takahashi, H, Watanabe, M

    Plant Biology 2009　2009/07/18

  14. Proteomics analysis of Synechocystis sp. strain PCC6803 in response to high light International-presentation

    Nahoko Higashitani, Atsushi Higashitani

    Plant Biology 2009　2009/07/18

  15. Checkpoint and physiological apoptosis in spaceflown C. elegans International-presentation

    Higashitani A, Higashibata A, Sasagawa Y, Sugimoto T, Miyazawa Y, Szewcyk NJ, Viso M, Gasset G, Eche B, Fukui K, Shimazu T, Fujimoto N, Kuriyama K, Ishioka N

    ESA international conference “Science on European Soyuz Missions to the International Space Station.”　2006/06/27

  16. Analyses of entire gene expression during high-temperature injuty to anther development in barley plants International-presentation

    Oshino T, Abiko M, Ichiishi E, Saito R, Higashitani A

    20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress　2006/06/18

  17. The decrease in myosin found in muscle developed during spaceflight is transcriptionally regulated. International-presentation

    Higashibata A, Szewczyk NJ, Conley CA, Imamizo-Sato M, Higashitani A, Ishioka N

    20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress　2006/06/18

  18. Characterization of C. elegans mtSSB gene and construction of mitochondria-deficient animals International-presentation

    Sugimoto T, Ichiishi E, Saito R, Higashitani A

    20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress　2006/06/18

  19. 日本放射線影響学会若手放射線生物研究会合同シンポジウム 「線虫を用いたDNA損傷応答、修復の分子機構の解明」 International-presentation

    日本組織培養学会創立50周年記念国際シンポジウム　2006/05/24

  20. Characterization of C. elegans par2.1 gene and construction of mitochondria-deficient animals International-presentation

    Sugimoto T, Higashitani A

    International Conference on Mitochondria and Life 2005　2005/12/14

  21. RNA interference and protein phosphorylation in space environment using the nematode Caenorhabditis elegans International-presentation

    C. elegans Model Specimens in Space (CEMSS 2A)　2005/06/30

  22. Workshops: Worms in Space- ICE-First and the Future International-presentation

    Johnsen B, Viso M, Ishioka N, Conley C, Szewczyk N, Segalat L, Higashitani A, Rose A

    15th International C. elegans Meeting.　2005/06/25

  23. Checkpoint and physiological apotosis in germ cells proceeds normally in spaceflown Caenorhabditis elegans on the ICE first experiment. International-presentation

    Higashitani A, Higashibata A, Sasagawa Y, Sugimoto T, Miyazawa Y, Szewcyk NJ, Viso M, Gasset G, Eche B, Fukui K, Shimazu T, Fujimoto N, Kuriyama K, Ishioka N

    15th International C. elegans Meeting.　2005/06/25

  24. Microgravity-dependent alteration of protein and gene expression in Caenorhabditis elegans on the international C. elegans space experiment-first (ICE-first). International-presentation

    Ishioka N, Higashibata A, Higashitani N, Seki M, Sato(Imamizo) M, Szewcyk N, Viso M, Gasset G, Eche B, Fujimoto N, Kuriyama K, Fukui K, Shimazu T, Higashibata A

    15th International C. elegans Meeting.　2005/06/25

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Research Projects 33

  1. 宇宙環境が生物に及ぼす影響に関する研究 Competitive

    System: Funded Research

    2003/04 - Present

  2. オオムギを用いた高温障害の研究 Competitive

    System: Grant-in-Aid for Scientific Research

    2000/03 - Present

  3. Studies of the effect of radiation on germ line cells Competitive

    System: Grant-in-Aid for Scientific Research

    2000/03 - Present

  4. Studies of gametogenesis and meiosis Competitive

    System: Grant-in-Aid for Scientific Research

    2000/03 - Present

  5. Stress responses in animal and plant Competitive

    System: Grant-in-Aid for Scientific Research

    2000/03 - Present

  6. Elucidation of Mechanism of Pseudo-Gravitational Effects on Plants by Reactive Species Generated by Circulating Atmospheric Pressure Plasma

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Challenging Research (Exploratory)

    Institution: Tohoku University

    2024/06/28 - 2027/03/31

  7. Mitochondrial mechanobiology to unravel its role in muscular atrophy Competitive

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2018/04/01 - 2023/03/31

  8. Mechano-nutrition-signaling for disuse muscle atrophy

    NIKAWA Takeshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: The University of Tokushima

    2019/04/01 - 2022/03/31

    More details Close

    In this study, we investigated the effect of oxidative stress on aconitase activity. Clinorotation inhibited aconitase activity and enhanced the ubiquitin-proteasome system proteolytic pathway. N-Acetylcysteine (NAC) treatment restored this inhibited aconitase activity and suppressed the ubiquitin-proteasome-based proteolytic pathway. In addition, NAC treatment suppressed mitochondrial division, which is increased by clinorotation. Next, mice subjected to tail suspension for 2 weeks were allowed to drink 0.3% NAC solution, and the effect of suppressing muscle atrophy was examined. The hind limb muscles of the mice that drank the NAC solution had significantly suppressed muscle atrophy as compared with the group that drank the water. In addition, the aconitase activity in the muscle was also restored by NAC administration. Based on these results, it was considered that aconitase in the TCA cycle is likely to be a target enzyme for oxidative stress induced by disuse muscle atrophy.

  9. Mitochondrial mechanobiology to unravel its role in muscular atrophy Competitive

    Offer Organization: Japan Agency for Medical Research and Development

    System: Advanced Research and Development Programs for Medical Innovation

    Category: AMED-CREST

    Institution: Tohoku University

    2016/10 - 2022/03

  10. Integral Understanding of Life-regulation Mechanism from "SPACE"

    Furukawa Satoshi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: Japan Aerospace EXploration Agency

    2015/06/29 - 2020/03/31

    More details Close

    During a long-term stay in space, physical stress of zero gravity causes muscle atrophy and bone loss, psychological stress by an enclosed environment brings about biological rhythm dysfunction, environmental risks like space radiation exposure induce DNA mutation. These issues need to be resolved in an era of challenging an ultra-long-term stay in space with human exploration to Mars. At the same time, deep understanding of the issues will help contribute to maintaining homeostasis of life in our super-aged, stressful society on Earth, leading to healthy aging and longevity. Our team of experts attempted to integrally understand the plasticity and failure of life forms on Earth. Nearly 300 peer-reviewed scientific papers were published in the 5-year research period. Joint researches were conducted with more than 12 foreign research institutes from more than 6 countries. Young researchers were fostered.

  11. Mechanisms of mechanostress transmissions from nerves to muscles - metabolisms and adaptive responses

    Higashitani Atsushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Institution: Tohoku University

    2015/06/29 - 2020/03/31

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    The purpose of this study is to investigate the mechanism by which the C. elegans mechanosensor molecule/neuronal system adapts to changes in muscle and metabolism under space experiments and various culture conditions on the ground. As a result, in C. elegans grown under space microgravity, both gene and protein expression levels for muscular thick filaments, cytoskeletal elements, and mitochondrial metabolic enzymes decreased relative to parallel cultures on the 1G centrifuge. In addition, a growth factor BMP released from motor nerves and sensory nerve dopamine are reduced in the spaceflown C. elegans. Furthermore, the mechanism from mitochondrial damage to muscle atrophy has been demonstrated that Ca2+ overaccumulation in the muscular cells, activation of furin and MMPs, and ECM degradation are the major processes of muscle collapse.

  12. Development of new genetic resources of rice against global climate change

    Higashitani Atsushi, ODA Susumu, TOMODA Aya, HOSONUMA Nobutaka, SHAO Zhenhua, ZHANG Lin

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2015/04/01 - 2018/03/31

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    We performed genome and post genome analyses using Oryza sativa japonica cv. "Sasanishiki" and "Hitomebore". The former is susceptible to cold injury and panicle blast disease, and the latter is tolerant to both injuries. We here found that qCT-7 involved in the tolerance to cold injury did not link to the tolerance against panicle blast disease. In addition, <BR> Through these studies, we aim to develop new genetic resources that contribute to the stable supply of rice under abnormal weather on a global scale.

  13. Solar radiation-induced chloroplast damage and the clearance systems of damaged chloroplasts including autophagy

    IZUMI Masanori, HIDEMA Jun, HIGASHITANI Atsushi, NAKAMURA Sakuya

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    2014/04/01 - 2017/03/31

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    In this study, we focused on the chloroplast damage due to solar radiation and the clearance system of damaged chloroplasts in Arabidopsis plants. The data supports three major conclusions as follow; (i) the property of chloroplast damage varies due to the type of radiation, (ii) autophagy plays an important role in plant response to UVB damage, (iii) Photodamage induces the digestion of entire collapsed chloroplasts by an autophagy process termed chlorophagy.

  14. Exploration of the molecules responding to space environment and the critical points in development of nematodes

    Higashibata Akira, HIGASHITANI Atsushi, HASHIZUME Toko, UMEHARA Masumi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Japan Aerospace EXploration Agency

    2014/04/01 - 2017/03/31

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    In the past space flight experiments, we observed the effects of microgravity on the genes expression related with muscles and energy metabolisms. The purpose of this study is to know the molecules that were influenced by gravity changes, and to investigate the critical points on the developmental process of Caenorhabditis elegans known as a model organism. The worms were grown under artificial microgravity condition on a clinostat that was an apparatus widely used for experiments for gravity effects on the ground. The expression level of body morphogenesis related genes showed the changes in the early developmental phase by clinorotation. The result suggested that the body morphogenetic process was sensitive against the gravity changes. During the latter half of the developmental phase of the worms, the artificial microgravity effects were observed on the molecules related with protein phosphorylation that had been also observed in the space experiments.

  15. Development of optogenetics visual servo microscope

    Hashimoto Koichi, HIGASHITANI ATSUSHI, KAGAMI SHINGO, ARAI SHOGO

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Category: Grant-in-Aid for Scientific Research (A)

    Institution: Tohoku University

    2013/05/31 - 2017/03/31

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    In this study, in order to analyze the brain function mainly by the sensory response behavior system, we simultaneously stimulated neuronal activities, developed an external stimulus according to the state, and developed a system to measure behavior. In particular, as the integration platform, we developed the world's first robot microscope "OSACaBeN" that automatically tracks moving observation objects at high speed to stimulate specific nerve cells by projection mapping, and the properties of multiple dopamine cells in nematodes during action are different from each other. Furthermore, regarding the smell response behavior of nematodes, we clarified the possibility of possessing brain functions common to humans and others, performing time-integrated information "decision making".

  16. Study of molecular coordination between nuclear and mitochondrial replications.

    HIGASHITANI Atsushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2009 - 2012

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    ATR is highly conserved in all eukaryotes and functions as a cell-cycle nuclear checkpoint kinase. In mammals, ATR hypomorphic mutation causes a complex disease known as Seckel syndrome. However, molecular mechanisms that cause a wide variety of symptoms including accelerated aging have remained unclear. We here found that in the nematode Caenorhabditis elegans, a deletion mutant of ATR atl-1 achieved longevity. Transcription levels of certain superoxide dismutase genes significantly increased in the mutant. In addition, mammalian ATR over-expression induced autophagic cell death.

  17. 地球環境変動に伴う作物不稔現象の克服研究

    東谷 篤志

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 挑戦的萌芽研究

    Category: 挑戦的萌芽研究

    Institution: 東北大学

    2009 - 2010

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    植物の生殖成長の過程は栄養成長の過程に比べて、様々な環境ストレスに対してより感受性が高く、なかでも雄性配偶子である花粉形成は最もその影響を受けやすい。その結果、自然界において雄性不稔が広く観察されている。本研究では、オオムギならびにシロイヌナズナを用いて高温障害による花粉形成の分子基盤の解明と、イネ低温障害「冷害」の実験系の確立を行なうとともに、それらの原因の探索、そして、克服に目指した研究を進めた。 その結果、昨年度までのオオムギならびにシロイヌナズナによる高温障害に、葯特異的なオーキシンの低下が深く関わる現象の発見と、今年度、それら低下は細胞分裂、複製に関わる遺伝子、ミトコンドリアに関連する遺伝子群とそれぞれ共発現の関係にあり、オーキシンの低下がこれら遺伝子発現に密接に関与することを明らかにした。 イネの冷害に関しては、ジベレリンの影響がより強くみられることを、ジベレリン生合成の変異系統、ジベレリン応答性の変異系統、ならびにジベレリン処理、ジベレリンの阻害剤処理などを用いて、明らかにすることに成功した。さらに、低温に弱いササニシキと低温に強いヒトメボレの二系統を用いて、それら低温感受性に関わる遺伝因子の特定をQTL解析ならびに各種形質の相関を調べることにより実施した。その結果、低温環境下でも花粉数を多く作れる形質は低温耐性であること、一方、低温環境下で一穂あたり穎花を多く作れる形質は逆に低温感受性となること、QTL解析の結果、低温耐性(低温でも種子結実率が高い)と一穂あたりの穎花数(少ない形質)が第7染色体上の同一領域にリンクすることが確認された。従って、多収性のイネの形質は、低温などの障害には、逆にリスク要因になることが示された。

  18. 高温により誘導される雄性不稔実行プログラムの解明

    東谷 篤志

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特定領域研究

    Category: 特定領域研究

    Institution: 東北大学

    2007 - 2008

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    申請者は、DNAマイクロアレイを用いて、オオムギはるな二条の高温障害時の網羅的な遺伝子発現の変動ならびに生殖成長が進展する過程での発生分化に伴う発現変動について、はじめに明らかにし、論文発表した。また、研究支援班の協力により、我々が単離した六条オオムギと親のはるな二条を用いて、それぞれ高温障害時にみられる遺伝子発現の網羅的な解析を行った。その結果、二条と六条を比較して、通常の二条で高く発現している遺伝子の多くが、高温障害時に発現誘導されるこが明らかになった。これらは、本来、六条から二条になる過程で、穎花が退化するのに必要な遺伝子発現と高温障害時の遺伝子発現に一部共通性がみられることを示したものと考えられる。 また、高温は葯特異的にオーキシンの低下を引き起こすこと、その結果、オーキシンシグナル伝達が抑制されることを、オオムギとシロイヌナズナを用いて明らかにすることができた。

  19. Molecular Mechanisms for Rice Male Sterility under High Temperature Conditions

    KAWAGISHI Makiko, HIGASHITANI Atsushi, WATANABE Masao

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: National Agricultural Research Organization

    2007 - 2008

  20. モデル生物を利用したミトコンドリアゲノムの安定性に関する新規遺伝子群の探索

    東谷 篤志

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 萌芽研究

    Category: 萌芽研究

    Institution: 東北大学

    2007 - 2008

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    線虫の細胞周期チェックポイント制御に関わるATL1(ヒトATRの相同的遺伝子)が、ミトコンドリアのDNA複製の制御に深く関わることを、突然変異系統ならびにそのRNAiによる発現抑制個体を用いて、今回、世界に先駆けて明らかにすることが出来た。さらに、この原因がDNA複製の基質やその合成に深く関わるribonucleotide riductaseの発現調節などに関わるものでないことも明らかにした。この研究成果は、Genetics. 2008 180(1):681-6. に発表するに至った。また、ミトコンドリア損傷の分子影響と放射線照射時の影響、強磁場暴露の影響とそれぞれDNAマイクロアレイを用いて、全ゲノムの網羅的な遺伝子発現を解析することで、比較解析を行った。 さらに、ヒト培養細胞を用いた研究を行い、上記の核のチェックポイント制御に関わる遺伝子がミトコンドリアの維持にも同様に関与するのかについて調べた結果、ヒトATR遺伝子が人為的な破壊細胞では、ミトコンドリアDNAのコピー数の低下は認められなかったが、転写やタンパク質間の新規相互作用を含め、核とミトコンドリアの両方に関して、ある種の連携が共通にみられることが明らかになった。

  21. Study of effect of ionizing radiation on germline cells

    HIGASHITANI Atsushi, KOBAYASHI Yasuhiko

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2004 - 2007

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    To investigate positional effects of radiation with an energetic heavy-ion microbeam on germline cells using an experimental model metazoan Caenorhabditis elegans. The germline cells were irradiated with raster-scanned broad beam or collimated microbeam of 220MeV 12C5t particles delivered from the azimuthally varying field (AVF) cyclotron, and subsequently observed for cell cycle arrest and apoptosis. Whole-body irradiation with the broad beam at the L4 larval stage arrested germ cell proliferation. When the tip region of the gonad arm was irradiated locally with the microbeam at the L4 stage, the same arrest was observed. When the microbeams were used to irradiate the pachytene region of the gonad arm, at a young gravid stage, radiation-induced apoptosis occurred in the gonad. In contrast, arrest and apoptosis were not induced in the non-irradiated neighboring region or the opposite gonad. Similar results were confirmed in the c-abl-1 (mammalian ortholog of cellular counterpart of Abelson murine leukemia virus) mutant that is hypersensitive to radiation-induced apoptosis. In addition, we demonstrated that several genes encoding apoptotic cell-death activators and secreted surface proteins were upregulated after ionizing radiation. Some of them were also upregulated by defect of mitochondrial DNA replication. These results indicate that the microbeam irradiation is useful in characterizing tissue-specific, local biological response to radiation in organisms. DNA damage-induced cell cycle arrest and apoptosis were observed in locally irradiated regions, but there was little, if any, ''bystander effect'' in the nematode.

  22. モデル生物を用いた発ガンと防御に関する研究 Competitive

    System: Millennium Project

    2000/04 - 2005/03

  23. 野生イネへの窒素固定エンドファイトの内生および窒素固定活性に関するQTL解析

    佐藤 雅志, 南澤 究, 東谷 篤志, 石井 尊生

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 萌芽研究

    Category: 萌芽研究

    Institution: 東北大学

    2003 - 2005

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    この研究の目的は、野生イネと栽培イネとの交雑後代自殖系統集団を用い、野生イネ組織内から単離された窒素固定能を持つエンドファイトの野生イネ組織内への感染、コロニー形成、窒素固定活性を支配している遺伝子の数および座位を、量的遺伝子座(QTL)解析法を用いて解明することである。 佐藤は、窒素固定活性を支配するQTLの解析にそなえて最適の交雑自殖後代系統集団を選ぶために、野生イネ系統および栽培イネ系統に内生させたHerbaspirillum sp.B501の窒素固定酵素ニトロゲナーゼを構成する遺伝子nifHの発現およびアセチレン還元活性を再調査した。その結果、栽培イネ系統および野生イネ系統間にアセチレン還元活性の統計的な崔を再現することができなかった。 南澤は、野生イネ系統に内生させたHerbaspirillum sp.B501のnifHの発現を調べ、明期に発現の上昇かあり暗期には発現の低下が認められることを見出した。このnifH発現の明暗サイクルについては国際誌に発表した。 東谷は、栽培イネKasalathから単離された接種菌以外の細菌を16srRNAの塩基配列により同定した結果、イネのエンドファイトとしては初めて検出されたStenotrophomonas maltophiliaであることが見出された。 石井は、これまでと同様に栽培イネと野生イネとの戻し交雑後代(O.sativa Nipponbare///O.rufupogon W630/Nipponbare//Nipponbare, O.sativa IR36///O.rufupogon W630/IR36//IR36, Nipponbare///O.meridionalis W1627/Nipponbare//Nipponbare, IR36///O.meridionalis W1627/IR36//IR36)BC2由来のQTL解析集団の作出を引き続きすすめた。作出された集団に関してはDNAマーカーを用いて遺伝子型データベースの構築をすすめた。

  24. Characterization ofgenes involved in genomic stability using an experinental model organism.

    HIGASHITANI Atsushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Priority Areas

    Category: Grant-in-Aid for Scientific Research on Priority Areas

    Institution: Tohoku University

    2001 - 2004

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    Many human cancers originate from defects in the DNA damage response and the cell cycle control. These defects cause instability of chromosomes. To identify and characterize novel genes of DNA damage response and the cell cycle control, we used an experimental model organism the nematode Caenorthabditis elegans. In eukaryotic cells, members of the ataxia telangiectasia mutated gene (ATM) family play important roles in conserved signal transduction pathways for the DNA damage response. We have identified an ATM-like Ce-atl-1 in the C. elegans genome. Depletion of its gene product by RNA-mediated interference (RNA1) caused pronounced defects in chromosomal segregation and DNA damage responses such as repair, apoptosis, and G1 arrest From the results of yeast two-hybrid analysis, its N-terminal half physically interacted with PCNA and its C-terminal half interacted with several gene products related to transport of ER, endocytosis, transcriptional factor, single strand binding protein SSB. Some of them were essential for apoptosis, but dispensable for DNA repair process. We also analyzed the Ring-H2 finger protein RBX1 that is a common subunit of SKP1-CDC53/CUL1-F box (SCF), other Cullins and von Hippel-Lindau (VHL) tumour suppressor E3 ubiquitin ligase complexes. The RBX1 RNAi experiment suggested that the RBX1 protein participates in diverse functions relevant to mitotic chromosomal condensation and segregation, G1/S progression and cytokinesis. All these defects of RB×1 RNAi were consistent with those found after either depletion of CUL2, Elongin B (ELB1) or Elongin C (ELC1). In addition, direct interactions between ELC1 and CUL2, and ELC1 and ELC2, respectively, were revealed by bacterial two-hybrid analysis. Thus, the RB×1/CUL2/ELC1/ELB1 complex acts as an E3 ubiquitin ligase in C. elegans and is essential for diverse functions relevant to chromosomal dynamics and cell cycle control. These phenomena are probably ubiquitous in human cancer, because all these genes identified in this study are highly conserved in mammals.

  25. Adaptive strategies of plants to drought and high-temperature conditions and their control for plant cultivation in arid area

    TAKAHASHI Hideyuki, FUJII Nobuharu, HIGASHITANI Atsushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Category: Grant-in-Aid for Scientific Research (B)

    Institution: Tohoku University

    2001 - 2003

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    To obtain fundamental knowledge on the adaptive strategies of plants and to develop new methods and plants that could be used for plant cultivation in arid area, we studied the mechanisms for deep-sowing tolerance of seedlings, root hydrotropism, and high-temperature-induced male sterility. Wheat seedlings emerge from the soil by elongating their first internodes. A variety of wheat tolerant to deep-sowing conditions was more sensitive to gibberellin (GA); the first internodes treated with GA elongated longer than 40 cm in the dark. Accordingly, the seedlings successfully emerged even from 40-cm depth in the soil. This GA response of the wheat was achieved by accompanying simultaneous uptake of potassium from the culture media for cellular osmoregulaiion and spiral growth for reducing mechanical impedance in the soil. We established an experimental system for the study of hydrotropism in Arabidopsis roots and revealed the involvement of abscisic acid, auxin and waving response with hydrotropism. In addition, we found that moisture gradient or water stress caused immediate degradation of the starch anchors, amyloplasts, in columella cells of Arabidopsis roots, which reduced the responsiveness of roots to gravity and thus allowed them to exhibit hydrotropism without interference by gravitropism. Also, we isolated unique hydrotropic mutants of Arabidopsis, which enabled us to dissect molecular mechanism for hydrotropism in seedling roots. We found that high temperature during floral organogenesis induced male sterility in barley. In relation to the male sterility, high temperature inhibited the expression of histone H3,H4 and glycine-rich RNA binding protein genes whose expressions are dramatically induced just before anther development. Furthermore, it was suggested that the early development and differentiation of barley anthers are hypersensitive to high temperature stress due to the inhibited transcriptions.

  26. チェックポイント制御機構による発がん防御の分子解明(モデル生物・線虫を用いた基礎的研究)

    東谷 篤志

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 特定領域研究(C)

    Category: 特定領域研究(C)

    Institution: 東北大学

    2000 - 2000

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    ヒトATM(ataxia telangectasia mutated gene)遺伝子の変異疾患においては、免疫不全、生殖不全の他、高頻度に悪性腫瘍を発症することが知られている。本遺伝子は、細胞周期のチェックポイント制御に関わり、酵母からヒトに至る真核生物で広く保存されていることが近年明らかにされてきた。そこで本研究では、モデル生物の一つである線虫(Caenorhabditis elegans)を用いて、ATM様遺伝子とその下流に位置するとされるCHK2様遺伝子を単離し機能解析を行い、DNA損傷時の細胞周期チェックポイント制御機構を個体レベルで解明し、発ガン防御の基礎的な知見を得ることを目的とした。その結果、線虫Ce-atl-1(C.elegans ATM/ATR like 1)が、体細胞分裂ならびに減数分裂時の染色体の維持・安定性に重要な役割を担うこと、本遺伝子の発現抑制個体においては、紫外線などのgenotoxinに高感受性となること、一方、線虫Ce-chk-2は、減数分裂期の相同染色体間の対合の制御に必須で、体細胞分裂時のチェックポイント制御機構には関与しないことを見出した。またこれら研究を行った際に、線虫の減数分裂パキテン期の核が、体細胞分裂を行っている核に比べ、放射線に対して大変強い抵抗性を示し、この原因が相同的遺伝子組換え酵素(Ce-rdh-1:C.elegans RAD51,DMC1/LIM15 homolog 1)の高発現に起因することを見出した。

  27. Molecular analysis of control mechanisms for meiosis.

    HIGASHITANI Atsushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: Tohoku University

    1998 - 2000

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    In this study, we have carried out characterizations of a recA-like, gene Ce-rdh-1, ATM (ataxia telangiectasia mutated gene)-like gene Ce-atl-1 and Chk2 (check point gene 2)-like gene Ce-chk-2 from the nematode Caenorhabditis elegans. Ce-rdh-1 gene was essential for meiotic recombination and for repair of DNA double-strand breaks (Takanami et al., 1998, 2000). Ce-chk-2 gene encoded an essential protein kinase to form meiotic chromosome synapses. The repression of the Ce-chk-2 gene expression resulted in the abnormal and unpaired chromosomes of pachytene nuclei, the loss of meiotic recombination and the phenotype of achiasmata of diplotene and diakinesis nuclei. However, Ce-chk-2 gene was dispensable for repair process of DNA double-strand breaks (Higashitani et al., 2000). On the contrary, Ce-atl-1 gene was necessary for DNA repair process and the sensitivity to UV irradiation was increased by the repression of Ce-atl-1 gene. The repression of Ce-atl-1 gene also increased the incidence of somatic mutations, the extensive embryonic lethality, and the aneuploidy and chromosome instability during mitosis and meiosis. It indicates that Ce-atl-1 acts as a monitoring enzyme for DNA damage checkpoint control (Aoki et al., 2000). We have also carried out characterization of the effects of high-temperature stress on the development of pollen mother cells (PMCs) and microspores in barley plant (Hordeum vulgare L). When plants were exposed to high temperature for five days at the pre-meiotic stage, PMCs and tapetum cells failed to develop. The panicles at the heading stage had a normal appearance, but no pollen grains in their abnormal anthers were observed and their seeds were virtually sterile (Sakata et al., 2000).

  28. Plant growth and cultivation under environmental stresses

    TAKAHASHI Hideyuki, FUJII Nobuharu, NISHIZAWA Takeaki, HIGASHITANI Atsushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B).

    Category: Grant-in-Aid for Scientific Research (B).

    Institution: Tohoku University

    1997 - 2000

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    We have studied the mechanisms useful for the adaptation of plants to the conditions of environmental stresses. (1) We found a new gravimorphism of plants using agravitropic mutants of rice and barley. Furthermore, we discovered that gravimorphogenesis of cucurbitaceous seedlings was negatively controlled by gravity, which was accounted by the threshold theory of auxin concentration. By cloning and expression analysis of auxin-inducible genes and its transcriptional factor, we established a working hypothesis for the auxin regulation of the gravity's negative control. (2) A variety of wheat, Hong Mang Mai, was found to be tolerant to deep-seeding conditions in semi-arid area because it was super-sensitive to gibberellins in inducing strong elongation of the first internode. Surprisingly, the presence of potassium in culture media was absolutely required for the GA-induced elongation. (3) We established the experimental system for the study of hydrotropism in the roots of cucumber and Arabidopsis. It was found that auxin transport and distribution, calcium ion, abscisic acid and endo-xyloglucan transferase played important roles in root hydrotropism. (4) We established a model system for the study of the high-temperature-induced injury of the reproductive growth in barley. It was suggested that the expression of a calcium-dependent protein kinase was involved in the male sterility caused by high-temperature stress. We also discovered some genes that could be induced or suppressed in response to high temperature by adopting the serial analysis of gene expression. We have cloned several genes responsible for above responses of plants, which accounted, at least in part, for the mechanisms of plant adaptation to particular environment such as gravitational field and arid area. The accomplishments of the present study may lead to the development of new plants and regulatory methods of plant growth for improving crop yield.

  29. Regulatory mechnism of cell growth in SOS response of Escherichia coli

    HIGASHITANI Atsushi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Category: Grant-in-Aid for Scientific Research (C)

    Institution: National Institute of Genetics

    1994 - 1996

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    Regulatory mechnisms of cell growth in the SOS response of E.coli were investigated with emphas is on SulA protein, an SOS-inducible cell-division inhibitor. Direct interaction of SulA with FtsZ,which plays a central role in bacterial cell division was studied. Molecular dissection of SulA and the role of single-stranded DNA as a primary signal for SOS induction in vivo were also analyzed. 1.Using purified SulA protein that was fused to the maltose binding protein, I demonstrated in vitro that SulA interacts with FtsZ to from a stable complex. The reaction required GTP and its hydrolysis. The complex was formed in a molar ratio of approximately one to one of the two proteins. 2.From mutation analyzes, Arg 62, Leu 67, Trp 77, and Lys 87 in the central region of SulA were found essential for the cell-division inhibitory activity. N-terminal residues of SulA ranging from the 3rd to the 27th amino acid and C-terminal 21 residues were dispensable for the activity. The C-terminal 20 residues of SulA were enough for its recognition by and for complexformation with Lon protease. They were necessary, but noy enough, for degradation of SulA by Lon protease. 3.Infection of E.coli with mutant filamentous phage that are defective in the initiation of minus-strand DNA snythesis induced the SOS response as monitored by cellular level of LexA.This observation demonstrated that single-stranded DNA serves as a primary signal for SOS induction in vivo.

  30. DNA-PROTEIN INTERACTION IN DNA REPLICATION

    HORIUCHI Kensuke, NISHIMURA Yukinobu, NISHIMURA Akiko, HIGASHITANI Atsushi, HARA Hiroshi, YASUDA Seiichi

    Offer Organization: Japan Society for the Promotion of Science

    System: Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (A)

    Category: Grant-in-Aid for General Scientific Research (A)

    Institution: NATIONAL INSTITUTE OF GENETICS

    1990 - 1993

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    1) We studied protein-DNA interactions in initiation of DNA replication of filamentous bacteriophages of E.coli. In relation to replication of the leading (plus) strand, we found that (a) the initiation frequency is a function of negative superhelical density of template DNA, (b) the initiator protein (gpII) specifically binds and bends DNA in a stepwise fashion, (c) this leads to melting of duplex in the nicking region, (d) the melting is a prerequisite for the initiation, and (e) negative superhelicity is required for the melting to occur. The binding of gpII to DNA is co-operative and involves protein-protein interactions. These results indicate that the initiation of replication is preceded by an ordered series of protein-induced DNA conformational changes. The replication enhancer region contains a strong binding site for the host IHF protein, which bends DNA.Our results indicate that interaction between two regions flanking the IHF site is essential for the function of the enhancer. Replication of the lagging (minus) strand is initiated by a RNA primer that is synthesized by the host RNA polymerase. We studied the interaction between the enzyme and the origin DNA.The results suggested that there is certain similarity in recongnition mechanisms between initiation of transcription and primer synthesis. We also found that mutant phage that are defective in the origin for lagging strand synthesis induce the SOS response in the infected cells, and demonstrated that single-stranded DNA per se is the SOS signal in vivo. 2) We studied how a dnaK mutation in E.coli depresses initiation of chromosomal replication, and found that DnaK stabilizes the initiator protein DnaA in vitro. 3) We completed mapping of a large number of fts genes, which are involved in cell division. 4) We identified the natural promoter for penicillin-binding protein 3. This promoter appears to serve for transcription of a large operon that includes many genes involved in cell division.

  31. 大腸菌リン酸化蛋白質の解析

    東谷 篤志

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 国立遺伝学研究所

    1992 - 1992

  32. DNA複製における大腸菌IHF蛋白の役割

    堀内 賢介, 東谷 篤志, 安田 成一

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 重点領域研究

    Category: 重点領域研究

    Institution: 国立遺伝学研究所

    1990 - 1990

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    大腸菌のIHF(integration host factor)蛋白は、DNA上の特異的塩基配列に結合することによって、遺伝子発現、DNA複製、組換え等、さまざまな現象の制御にあずかることが知られている。我々は大腸菌繊維状ファ-ジのDNA複製がin vivoにおいてIHF蛋白によって正に制御されていることを見出したので、その作用機構を明らかにすることが本研究の目的である。 このファ-ジの複製開始域はcoreとenhancerの二領域から成り、coreには複製開始蛋白(gpII)が結合し、enhancerにはIHFの結合配列と思われるものが3個(左から順に1,2',2"と呼ぶ)存在する。Footprint法を用いた実験結果によれば、IHFは1には強く結合するが、2'及び2"には極めて弱い結合しか示さない。我々は欠失変異を用いて、複製の促進には1のみでなく、2'と2"を含む領域が必要なこと、また1と2'の間の距離を変化させると10塩基対の周期で複製頻度の変動がみられることを明らかにした。これは、IHFが1のみでなく2'もしくは2"への弱い結合によりDNAの高次構造変化を通じて複製にあずかることを示唆している。 我々はさきに、IHFに依存せずに複製できる変異ファ-ジを分離し、これらがgpIIの変異であることを明らかにしたが、本年度においてはこのような変異体の性質を詳細に研究した。特に、これら変異gpIIは野性型と異なり、負超螺旋度の低いDNAに対しても高いnicking活性を示すことを見出した。これらの結果は、(1)IHFが複製開始反応における最も早い段階、すなわちnickingによるprimer形成を制御すること、(2)IHFがDNAの超螺旋構造と類似の機構によって複製開始反応を促進することを示唆するものである。

  33. 真核生物の遺伝子組換え等に関する研究

    東谷 篤志

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 奨励研究(A)

    Category: 奨励研究(A)

    Institution: 名古屋大学

    1989 - 1989

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Social Activities 6

  1. 中学への出前授業「遺伝子をみよう」

    2003/08 - Present

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    遺伝子に関する講義と細胞から実際に抽出する実験指導

  2. 宮城第一高校 出前授業

    2017/05/22 -

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    「宇宙に生きる―生物と重力の関係―」

  3. 大西宇宙飛行士 国際宇宙ステーション（ISS）長期滞在ミッション報告会 in 宮城県仙台市

    2017/05/22 -

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    「国際宇宙ステーションでのモデル生物を利用した宇宙実験」公開講演

  4. 仙台第三高校 出前授業

    2017/01/17 -

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    「モデル生物 線虫の宇宙実験を通して」

  5. 武蔵野市寄付講座 現代教養特講 宇宙進出の最前線

    2016/10/19 -

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    「小さな宇宙飛行士線虫の冒険」公開講座

  6. 宮城第一高校 出前授業

    2016/05/23 -

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    「モデル生物 線虫の宇宙実験を通して」

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Media Coverage 9

  1. 宇宙で生命科学実験

    日刊工業新聞

    2015/01/27

    Type: Newspaper, magazine

  2. 科学の泉「宇宙の科学利用」計6回

    河北新報

    2014/11/11

    Type: Newspaper, magazine

  3. イネ「冷害」メカニズム解明

    河北新報

    2014/03/07

    Type: Newspaper, magazine

  4. 気温低下でホルモン減 イネの冷害、一端解明

    日経産業新聞

    2014/03/05

    Type: Newspaper, magazine

  5. RNA干渉や筋肉の衰え 宇宙で仕組み検証

    河北新聞

    2009/11/08

    Type: Newspaper, magazine

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    ＲＮＡ干渉や筋肉の衰え 宇宙で仕組み検証 東北大 東北大大学院生命科学研究科の東谷篤志教授（ゲノム継承システム）らの研究グループは、ＲＮＡの 断片を細胞内に入れ、遺伝子の働きを抑える「ＲＮＡ干渉」の効果を宇宙空間で検証する実験を近く始める。 併せて筋肉が衰える仕組みの解明にも挑み、人間が宇宙で生活を送る際の医療研究に役立てる。

  6. 宇宙実験 準備着々 東北大の3研究室

    読売新聞

    2005/12/30

    Type: Newspaper, magazine

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    国際公募などにより、東北大生命科学研究科の3研究室がそれぞれ、次期宇宙実験の機会を得て、その準備が進められていることの紹介記事。

  7. 発がん防ぐ機能担う「細胞死」 宇宙でもアポトーシス

    読売新聞

    2005/09/27

    Type: Newspaper, magazine

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    宇宙の微小重力環境下でも生物のアポトーシス（傷ついた細胞を自発的に死に至らす）の機構が正常に働くことを検証した。宇宙放射線などにより、DNAが激しく傷ついても細胞がそれらを除去することができる能力検証につながる。

  8. “細胞死”宇宙でも正常に

    NHKテレビ

    2005/09/18

    Type: TV or radio program

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    宇宙の微小重力環境下でも生物のアポトーシス（傷ついた細胞を自発的に死に至らす）の機構が正常に働くことを検証した。宇宙放射線などにより、DNAが激しく傷ついても細胞がそれらを除去することができる能力検証につながる。

  9. 宇宙放射線の人体影響探る

    河北新聞

    2004/04/30

    Type: Newspaper, magazine

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    モデル生物Cエレンガスを用いて、国際線虫共同実験に参画し、細胞死の有無を調査した研究

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Other 11

  1. 日本近海の海藻からの次世代サルコペニア予防食の創製

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    海藻におけるフラボノイドを活用して、新規、筋委縮を抑制する予防食の創製をめざす。

  2. 植物成長促進剤の作用機構の解明

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    植物成長促進剤に含まれる有効成分の分析とその作用機構を明らかにする。

  3. イネ用環境ストレス耐性付与資材

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    イネの冷害に効果を有する資材の開発

  4. 開花期低温ストレス耐性関連遺伝子の単離と機能解析

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    イネ科作物のイネならびにオオムギを用いて、温度ストレスに対する分子応答と生殖障害の発症機構の解明ならびに、耐性関連遺伝子の単離と機能解析を研究目的とする。

  5. モデル生物を利用した宇宙環境バイオドシメータの開発

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    モデル生物線虫Cエレガンスは、全ゲノム2万遺伝子が既に解読され、その4割はヒトにおいても保存され、細胞複製や発生、筋神経系、減数分裂をはじめとする配偶子形成などの生命基盤の共通性が示唆されている。従って、製薬過程で不可欠な工程の新規化合物の生物影響やその効果、変異原性などを調べることにも利用されはじめている。また、放射線や温度変化など各種のストレスに応答して発現誘導する遺伝子群については、当研究室をはじめ国内外の研究室で、マイクロアレイによる網羅的な解析がなされている。そこで本研究では、これまで物理的な方法に委ねられてきた宇宙放射線の測定において、生きた線虫試料や大腸菌などを用いて、微小重力など複合的な宇宙環境の要素による生物影響も加味することができるバイオドシメータを開発し、最終的には国際宇宙ステーションなどで放射線の環境バイオモニタリングシステムとして構築することを研究目的とする。

  6. モデル生物を用いた強磁場の生物影響に関する研究

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    本研究では、無冷媒型超伝導マグネット6T-CSM装置を用いて、内部温度を20～27℃まで制御しながら、野生型N2ならびにPC72(hsp-16::lacZ)系統2）の線虫における分子応答について解析を行った。今回使用したDNAマイクロアレイは、C. elegans Genome Array (Affymetrix社製)を用いて、線虫の全ゲノム遺伝子に対応する約22,150の転写産物の発現解析を行った。

  7. 宇宙フライトにより発現変動するゲノム遺伝子の網羅的な機能解析とデータベース構築

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    私達は昨年、線虫を用いたICE first実験に参画し、（１）宇宙環境下でのチェックポイント・アポトーシス、（２）網羅的な発現プロテオミクス解析等を行った。また、全ゲノム遺伝子に対するマイクロアレイ解析を実施し、組換え修復、チェックポイント・アポトーシス等の遺伝子発現について詳細に解析した。また、機能未知遺伝子も含め約250の遺伝子発現が有意に変動していたことを見出した。そこで本研究では、このマイクロアレイ結果を用いて、宇宙フライトにより発現が変動した遺伝子群の機能解析と、機能別クラスタリング、さらに各々に対応するヒトやマウスの相同的遺伝子の機能と発現情報を収集したデータベースを構築する。これら線虫における機能解析と哺乳類の同祖遺伝子の情報を含めたデータベース化により、微小重力をはじめとする宇宙フライト環境が、生物へ及ぼす分子影響について統合的に理解し、宇宙利用の先駆的な研究につなげたい。

  8. 線虫C. elegansを用いた宇宙環境におけるRNAiとタンパク質リン酸化

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    モデル生物の1つである線虫C. elegansを用いて、微小重力をはじめとする複合的な宇宙特殊環境が生物に及ぼす影響を、国際宇宙ステーション（ISS)を利用して、分子レベルで解明することを行う。

  9. 強磁場環境が及ぼす生物影響への研究：染色体DNAの切断ならびにアポトーシスへの影響について

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    本研究では、無冷媒型超伝導マグネット6T-CSM装置を主に用いて様々な静磁場強度のもとで、内部温度を20℃に保ち、Cエレガンスを6 cmプラスチックシャーレ内で大腸菌を塗布した寒天培地上で飼育を行った。今回用いたCエレガンスは、野生型N2とhim-172）、ならびにabl-1遺伝子（DNA損傷に依存したアポトーシスの誘導が野生型に比べ高頻度に増加する変異）３）の突然変異系統を用いて、発生、成育、染色体DNAの切断およびアポトーシス誘導の影響について調べた。また、約2万からなるCエレガンスの全ゲノム遺伝子DNA マイクロアレイを用い、強磁場環境が遺伝子発現に及ぼす影響についての網羅的な解析を実施した。

  10. 重イオンマイクロビームによる生殖細胞の放射線影響とバイスタンダー効果の研究

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    TIARAの重イオンマイクロビーム照射装置を用いて、線虫の生殖細胞形成における照射の影響、特にDNA損傷の修復、アポトーシス、そしてバイスタンダー効果に関する分子機構を明らかにすること。

  11. らん藻のタンパク質複合体のプロテオーム解析に関する研究

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    ゲノムの全配列情報が決定されたらん藻を用いて、タンパク質複合体のプロテオーム解析に関する実験研究の手法の確立を行う。

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