Details of the Researcher

PHOTO

Toshiyuki Kowada
Section
Institute of Multidisciplinary Research for Advanced Materials
Job title
Associate Professor
Degree

  • 博士（工学） (Kyoto University)

Research History 6

  • 2023/07 - Present
    Tohoku University　Institute of Multidisciplinary Research for Advanced Materials　Associate Professor

  • 2016/11 - 2023/06
    Tohoku University　Institute of Multidisciplinary Research for Advanced Materials　Assistant Professor

  • 2023/02 - 2023/03
    Tohoku University

  • 2014/10 - 2016/10
    School of medicine, Stanford University, USA　Postdoctoral Fellow

  • 2011/04 - 2014/09
    WPI Immunology Frontier Research Center, Osaka University, Japan　Specially appointed assistant professor

  • 2010/04 - 2011/03
    WPI Immunology Frontier Research Center, Osaka University, Japan　Specially appointed researcher

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Education 2

  • Kyoto University　Graduate School, Division of Engineering　Department of Energy and Hydrocarbon Chemistry

    2005/04 - 2010/03

  • Kyoto University　Faculty of Engineering　Division of Industrial Chemistry

    2001/04 - 2005/03

Committee Memberships 8

  • 化学フロンティア研究会　運営委員代表

    2012/03 - Present

  • UW- TU:AOS (University of Washington – Tohoku University : Academic Open Space)　化学分科会委員

    2022/11 - 2025/03

  • 日本化学会 生体機能関連化学部会 若手の会第32回サマースクール　世話人代表

    2019/09 - 2021/08

  • 日本化学会 生体機能関連化学部会　若手幹事

    2019/03 - 2021/02

  • 日本化学会 生体機能関連化学部会　若手幹事 代表

    2019/03 - 2020/02

  • 第13回バイオ関連化学シンポジウム　実行委員

    2019/09 - 2019/09

  • 日本化学会 生体機能関連化学部会　第7回バイオ関連化学シンポジウム若手フォーラム世話人

    2018/09 - 2019/09

  • 第23回日本バイオイメージング学会学術集会　運営委員

    2014/09 - 2014/09

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Professional Memberships 7

  • 日本分子イメージング学会

  • Chemical Frontier Workshop

  • Bioimaging Society

  • The Society of Synthetic Organic Chemistry, Japan

  • Japanese Society for Chemical Biology

  • Division of Biofunctional Chemistry, The Chemical Society of Japan

  • Chemical Society of Japan

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Research Interests 2

  • Chemical Biology

  • Biofunctional Chemistry

Research Areas 3

  • Nanotechnology/Materials / Synthetic organic chemistry /

  • Nanotechnology/Materials / Chemical biology /

  • Nanotechnology/Materials / Biochemistry /

Awards 6

  1. “Young Scholar Lecture” in the 103rd CSJ Annual Meeting (2023)

    2023/03　The Chemical Society of Japan

  2. Young Scientist Award in Image Science

    2023/01　Konica Minolta Science and Technology Foundation

  3. バイオ関連化学シンポジウム講演賞

    2021/09　Division of Biofunctional Chemistry & Division of Biotechnology, The Chemical Society of Japan　Development of small-molecule fluorescent probes for quantification of labile Zn2+ concentration and monitoring of Zn2+ dynamics in organelles

  4. Award for Research Proposal

    2019/12　The Society of Synthetic Organic Chemistry. Japan

  5. Young Research Award

    2019/09　Bioimaging Society

  6. The 8th Annual Meeting of Japanese Society for Chemical Biology, Poster Presentation Award

    2013/06　日本ケミカルバイオロジー学会

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Papers 34

  1. Zinc-redox crosstalk regulates proteostasis in the endoplasmic reticulum Peer-reviewed

    Yuta Amagai, Chihiro Arai, Wakana Yamamoto, Satoshi Watanabe, Toshiyuki Kowada, Roberto Sitia, Jun Hoseki, Shin Mizukami, Masaki Matsumoto, Kenji Inaba

    Nature Communications　2026/04/22

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41467-026-72250-w 　

    eISSN： 2041-1723

  2. Azopyrazole-based Photochromic Dimerizer Enables Optical Control of Cellular Signaling with Minimal Basal Activation International-journal

    Toshiyuki Kowada, Toshizo Sato, Kento Kasahara, Satoshi Hamaguchi, Konoka Baba, Takato Mashita, Muhammad Wildan Saifudin, Hayashi Yamamoto, Nobuyuki Matubayasi, Shin Mizukami

    ChemRxiv　2026/02/25

    Publisher: American Chemical Society (ACS)

    DOI： 10.26434/chemrxiv.10001881/v2 　

  3. Pathophysiological significance of impaired KAT7-dependent histone H3K14 acetylation during zinc deficiency International-journal Peer-reviewed

    Takao Fujisawa, Satoshi Takenaka, Lila Maekawa, Motoyuki Ogawa, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami, Yugo Kato, Michio Suzuki, Hisashi Noma, Isao Naguro, Hidenori Ichijo

    Nature Communications　17　(1)　1710　2026/02/17

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41467-026-69476-z 　

    eISSN： 2041-1723

  4. A Cell-Surface-Targeted Fluorogenic Probe for Detection of Sulfatase 2 Activity International-journal Peer-reviewed

    Yi Ding, Toshiyuki Kowada, Toshitaka Matsui, Norihiko Sasaki, Shin Mizukami

    Bioconjugate Chemistry　9　(36)　1980-1986　2025/08/23

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.bioconjchem.5c00251 　

    ISSN： 1043-1802

    eISSN： 1520-4812

  5. Zinc-redox crosstalk regulates proteostasis in the endoplasmic reticulum

    Kenji Inaba, Yuta Amagai, Chihiro Arai, Wakana Yamamoto, Satoshi Watanabe, Toshiyuki Kowada, Roberto Sitia, Jun Hoseki, Shin Mizukami, Masaki Matsumoto, Kenji Inaba

    Research square (preprint)　2025/08/11

    Publisher: Springer Science and Business Media LLC

    DOI： 10.21203/rs.3.rs-7251469/v1 　

    More details Close

    Abstract <p>Zinc homeostasis is crucial for various biological processes, including gene regulation, signal transduction, and proteostasis. ZIP7 is a membrane transporter that exports zinc ions (Zn2+) from the lumen of the endoplasmic reticulum (ER) to the cytosol, and its dysfunction causes ER stress, although the underlying mechanism remains unclear. Here, we show that ZIP7 inhibition increases the labile Zn2+ concentration in the ER to micromolar levels, approximately 106 times higher than its steady-state level. Such abnormally high Zn2+ concentrations disrupt the function and trafficking of the Zn2+-dependent chaperone ERp44 at the ER-Golgi interface. In vitro assays using recombinant proteins demonstrated that Zn2+ inhibits the Ero1α-PDI oxidative system, and that ERp44 enhances this inhibitory effect. Consequently, the ER redox environment becomes more reducing, severely impairing the oxidative folding of key membrane receptors such as Notch1 and EGFR. These findings reveal the essential role of zinc homeostasis in redox-dependent proteostasis within the ER.</p>

  6. Quantification of lysosomal labile Zn2+ and monitoring of Zn2+ efflux using a small-molecule–protein hybrid fluorescent probe International-journal Peer-reviewed

    Yuyin Du, Toshiyuki Kowada, EunHye Sung, Rong Liu, Andrei Soloviev, Toshitaka Matsui, Shin Mizukami

    Journal of Inorganic Biochemistry　264　112811-112811　2025/03

    Publisher: Elsevier BV

    DOI： 10.1016/j.jinorgbio.2024.112811 　

    ISSN： 0162-0134

  7. Pathophysiological Significance of Impaired KAT7-Dependent Histone H3K14 Acetylation During Zinc Deficiency

    Takao Fujisawa, Satoshi Takenaka, Lila Maekawa, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami, Yugo Kato, Michio Suzuki, Hisashi Noma, Isao Naguro, Hidenori Ichijo

    bioRxiv　2025/01/29

    Publisher: Cold Spring Harbor Laboratory

    DOI： 10.1101/2023.10.18.562865 　

    More details Close

    Summary Zinc is an indispensable micronutrient for optimal physiological functions, and zinc deficiency has been implicated in the pathogenesis of various human diseases. One potential mechanism underlying such pathogenic effects is the alteration of gene expression caused by zinc deficiency; however, the details of this process remain largely unexplored. Here, we show that during zinc deficiency, the histone acetyltransferase KAT7 loses its enzymatic activity, leading to the attenuated acetylation of histone H3 at Lys14 (H3K14ac) at enhancer regions. Physiologically, the decrease in H3K14ac leads to the upregulation of the expression of ZIP10, a plasma membrane-localized zinc transporter, thereby facilitating the import of extracellular zinc to maintain cellular zinc homeostasis. Moreover, prolonged zinc deficiency in mice induced by a zinc-deficient diet or high-fat diet, accompanied by decreased H3K14ac levels in the liver, upregulated the expression of genes associated with intracellular lipid droplet formation, leading to the accumulation of lipids within liver tissue. Our findings demonstrate that cells respond to zinc deficiency by converting it into an epigenetic signal that drives physiological or pathophysiological biological processes.

  8. Quantitative control of subcellular protein localization with a photochromic dimerizer International-journal Peer-reviewed

    Takato Mashita, Toshiyuki Kowada, Hayashi Yamamoto, Satoshi Hamaguchi, Toshizo Sato, Toshitaka Matsui, Shin Mizukami

    Nature Chemical Biology　20　1461-1470　2024/06/18

    Publisher: Springer Science and Business Media LLC

    DOI： 10.1038/s41589-024-01654-w 　

    ISSN： 1552-4450

    eISSN： 1552-4469

  9. Long-term Imaging of Intranuclear Mg2+ Dynamics during Mitosis Using a Localized Fluorescent Probe Peer-reviewed

    Yusuke Matsui, Toshiyuki Kowada, Yi Ding, Priya Ranjan Sahoo, Kazuya Kikuchi, Shin Mizukami

    Chemical Communications　59　7048-7051　2023/05

    Publisher: Royal Society of Chemistry (RSC)

    DOI： 10.1039/d2cc05930d 　

    ISSN： 1359-7345

    eISSN： 1364-548X

    More details Close

    A novel fluorescent Mg2+ probe was developed based on a small molecule–protein hybrid. This probe enables subcellular targeting, long-term imaging, and high selectivity for Mg2+ over Ca2+. Using ratiometric fluorescence...

  10. Zinc homeostasis governed by Golgi-resident ZnT family members regulates ERp44-mediated proteostasis at the ER-Golgi interface International-journal Peer-reviewed

    Yuta Amagai, Momo Yamada, Toshiyuki Kowada, Tomomi Watanabe, Yuyin Du, Rong Liu, Satoshi Naramoto, Satoshi Watanabe, Junko Kyozuka, Tiziana Anelli, Tiziana Tempio, Roberto Sitia, Shin Mizukami, Kenji Inaba

    Nat. Commun.　14　2683　2023/05

    DOI： 10.1038/s41467-023-38397-6 　

  11. Arylazopyrazole-Based Photoswitchable Inhibitors Selective for Escherichia coli Dihydrofolate Reductase International-journal Peer-reviewed

    Himadri S. Sarkar, Takato Mashita, Toshiyuki Kowada, Satoshi Hamaguchi, Toshizo Sato, Kento Kasahara, Nobuyuki Matubayasi, Toshitaka Matsui, Shin Mizukami

    ACS Chemical Biology　18　(2)　340-346　2023/01/20

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acschembio.2c00749 　

    ISSN： 1554-8929

    eISSN： 1554-8937

  12. Quantitative and Repetitive Control of Subcellular Protein–Protein Interaction Using a Photochromic Dimerizer International-journal

    Takato Mashita, Toshiyuki Kowada, Hayashi Yamamoto, Satoshi Hamaguchi, Toshitaka Matsui, Shin Mizukami

    ChemRxiv　2022/11/09

    DOI： 10.26434/chemrxiv-2022-7vq8s-v2 　

  13. Flexible Target Recognition of the Intrinsically Disordered DNA-Binding Domain of CytR Monitored by Single-Molecule Fluorescence Spectroscopy International-journal International-coauthorship Peer-reviewed

    Shrutarshi Mitra, Hiroyuki Oikawa, Divya Rajendran, Toshiyuki Kowada, Shin Mizukami, Athi N. Naganathan, Satoshi Takahashi

    The Journal of Physical Chemistry B　126　(33)　6136-6147　2022/08/15

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acs.jpcb.2c02791 　

    ISSN： 1520-6106

    eISSN： 1520-5207

  14. Clip to Click: Controlling Inverse Electron-Demand Diels–Alder Reactions with Macrocyclic Tetrazines International-journal Peer-reviewed

    Ira Novianti, Toshiyuki Kowada, Shin Mizukami

    Org. Lett.　24　(17)　3223-3226　2022/04

    DOI： 10.1021/acs.orglett.2c01010 　

  15. Organelle-Level Labile Zn2+ Mapping Based on Targetable Fluorescent Sensors Peer-reviewed

    Rong Liu, Toshiyuki Kowada, Yuyin Du, Yuta Amagai, Toshitaka Matsui, Kenji Inaba, Shin Mizukami

    ACS Sens.　7　748-757　2022/03/03

    Publisher: American Chemical Society (ACS)

    DOI： 10.1021/acssensors.1c02153 　

    ISSN： 2379-3694

    eISSN： 2379-3694

  16. Fluorescent Probes for the Quantification of Labile Metal Ions in Living Cells Invited Peer-reviewed

    Toshiyuki Kowada, Shin Mizukami

    Journal of Synthetic Organic Chemistry, Japan　79　(11)　1020-1032　2021/11/01

    Publisher: The Society of Synthetic Organic Chemistry, Japan

    DOI： 10.5059/yukigoseikyokaishi.79.1020 　

    ISSN： 0037-9980

    eISSN： 1883-6526

  17. Functional small-molecule probes for fluorescence imaging of osteoclasts in vivo International-journal Invited Peer-reviewed

    Toshiyuki Kowada

    Bioimages　29　1-10　2021/08

    DOI： 10.11169/bioimages.29.1 　

  18. Protocol for synthesis and use of a turn-on fluorescent probe for quantifying labile Zn2+ in the Golgi apparatus in live cells International-journal Peer-reviewed

    Toshiyuki Kowada, Tomomi Watanabe, Rong Liu, Shin Mizukami

    STAR Protocols　2　(2)　100395-100395　2021/06

    Publisher: Elsevier BV

    DOI： 10.1016/j.xpro.2021.100395 　

    ISSN： 2666-1667

  19. Optical manipulation of subcellular protein translocation using a photoactivatable covalent labeling system International-journal Peer-reviewed

    Toshiyuki Kowada, Keisuke Arai, Akimasa Yoshimura, Toshitaka Matsui, Kazuya Kikuchi, Shin Mizukami

    Angew. Chem. Int. Ed.　60　(20)　11378-11383　2021/02

    DOI： 10.1002/anie.202016684 　

  20. Quantitative Imaging of Labile Zn2+ in the Golgi Apparatus Using a Localizable Small-Molecule Fluorescent Probe International-journal Peer-reviewed

    Toshiyuki Kowada, Tomomi Watanabe, Yuta Amagai, Rong Liu, Momo Yamada, Hiroto Takahashi, Toshitaka Matsui, Kenji Inaba, Shin Mizukami

    Cell Chem. Biol.　27　1521-1531　2020/09/29

    Publisher: Elsevier BV

    DOI： 10.1016/j.chembiol.2020.09.003 　

    ISSN： 2451-9456

  21. Exploring Condensation Reaction Between Aromatic Nitriles and Amino Thiols to Form Nanoparticles in Cells for Imaging the Activity of Protease and Glycosidase Peer-reviewed

    Zixin Chen, Min Chen, Yunfeng Cheng, Toshiyuki Kowada, Jinghang Xie, Xianchuang Zheng, Jianghong Rao

    Angew. Chem. Int. Ed.　59　3272-3279　2020/01

    DOI： 10.1002/anie.201913314 　

  22. Light-wavelength-based Quantitative Control of Dihydrofolate Reductase Activity Using Photochromic Isostere of Inhibitor Peer-reviewed

    Takato Mashita, Toshiyuki Kowada, Hiroto Takahashi, Toshitaka Matsui, Shin Mizukami

    ChemBioChem　20　1382-1386　2019/01

    DOI： 10.1002/cbic.201800816 　

  23. Real-time intravital imaging of pH variation associated with osteoclast activity Peer-reviewed

    Hiroki Maeda, Toshiyuki Kowada, Junichi Kikuta, Masayuki Furuya, Mai Shirazaki, Shin Mizukami, Masaru Ishii, Kazuya Kikuchi

    Nat. Chem. Biol.　12　(8)　579-585　2016/08

    DOI： 10.1038/nchembio.2096 　

  24. BODIPY-based probes for the fluorescence imaging of biomolecules in living cells Peer-reviewed

    Toshiyuki Kowada, Hiroki Maeda, Kazuya Kikuchi

    Chem. Soc. Rev.　44　(14)　4953-4972　2015

    DOI： 10.1039/C5CS00030K 　

  25. Dynamic visualization of RANKL and Th17-mediated osteoclast function Peer-reviewed

    Junichi Kikuta, Yoh Wada, Toshiyuki Kowada, Ze Wang, Ge-Hong Sun-Wada, Issei Nishiyama, Shin Mizukami, Nobuhiko Maiya, Hisataka Yasuda, Atsushi Kumanogoh, Kazuya Kikuchi, Ronald, N. Germain, Masaru Ishii

    J. Clin. Invest.　123　(2)　866-873　2013/02

    DOI： 10.1172/JCI65054 　

  26. Electron-rich carbon nanorings as macrocyclic hosts for fullerenes Peer-reviewed

    Koji Miki, Tsuyoshi Matsushita, Yuki Inoue, Yoshinori Senda, Toshiyuki Kowada, Kouichi Ohe

    Chem. Commun.　49　(80)　9092-9094　2013

    DOI： 10.1039/c3cc42561d 　

  27. In Vivo Fluorescence Imaging of Bone-Resorbing Osteoclasts Peer-reviewed

    Toshiyuki Kowada, Junichi Kikuta, Atsuko Kubo, Masaru Ishii, Hiroki Maeda, Shin Mizukami, Kazuya Kikuchi

    J. Am. Chem. Soc.　133　(44)　17772-17776　2011/11/09

    DOI： 10.1021/ja2064582 　

  28. Near-infrared BODIPY dyes modulated with spirofluorene moieties Peer-reviewed

    Toshiyuki Kowada, Shuhei Yamaguchi, Hiroki Fujinaga, Kouichi Ohe

    Tetrahedron　67　(17)　3105-3110　2011/04/29

    DOI： 10.1016/j.tet.2011.02.073 　

  29. Synthesis of Strained Pyridine-Containing Cyclyne via Reductive Aromatization Peer-reviewed

    Koji Miki, Michiyasu Fujita, Yuki Inoue, Yoshinori Senda, Toshiyuki Kowada, Kouichi Ohe

    J. Org. Chem.　75　(10)　3537-3540　2010/05/21

    DOI： 10.1021/jo1006202 　

  30. Synthesis and Characterization of Highly Fluorescent and Thermally Stable pi-Conjugates involving Spiro[fluorene-9,4 '-[4H]indeno[1,2-b]furan] Peer-reviewed

    Toshiyuki Kowada, Kouichi Ohe

    Bull. Korean Chem. Soc.　31　(3)　577-581　2010/03/20

    DOI： 10.5012/bkcs.2010.31.03.577 　

  31. Synthesis, Structures, and Optical Properties of Heteroarene-Fused Dispiro Compounds Peer-reviewed

    Toshiyuki Kowada, Toshihisa Toshihisa, Kouichi Ohe

    J. Org. Chem.　75　(3)　906-913　2010/02/05

    DOI： 10.1021/jo902482n 　

  32. Highly Fluorescent BODIPY Dyes Modulated with Spirofluorene Moieties Peer-reviewed

    Toshiyuki Kowada, Shuhei Yamaguchi, Kouichi Ohe

    Org. Lett.　12　(2)　296-299　2010/01/15

    DOI： 10.1021/ol902631d 　

  33. Platinum- and Palladium-Catalyzed Sequential Reactions: Regioselective Synthesis of 9-Fluorenylidenes from 9-Ethynylfluoren-9-yl Carboxylates and Furans Peer-reviewed

    Koji Miki, Yoshinori Senda, Toshiyuki Kowada, Kouichi Ohe

    Synlett　(12)　1937-1940　2009/07/22

    DOI： 10.1055/s-0029-1217531 　

  34. Synthesis, characterization, and photoluminescence of thiophene-containing spiro compounds Peer-reviewed

    Toshiyuki Kowada, Yoshio Matsuyama, Kouichi Ohe

    Synlett　(12)　1902-1906　2008/07/16

    DOI： 10.1055/s-2008-1078571 　

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Misc. 9

  1. 局在型蛍光プローブによる細胞内遊離亜鉛の定量イメージングとその局所分布の機能的役割

    Shin Mizukami, Toshiyuki Kowada

    日本分子イメージング学会機関紙　19　(2)　8-15　2026/05

  2. 生細胞内タンパク質の局在を自在に操る－タンパク質ラベル化の光制御法の開発 Invited

    Toshiyuki Kowada

    CHEMISTRY & CHEMICAL INDUSTRY　77-1　034-035　2024/01

  3. 若手研究者からのメッセージ Invited

    小和田 俊行

    日本化学会バイオテクノロジー部会NEWS LETTER　25　(2)　15-20　2022/02

  4. 細胞小器官内遊離Zn2+の濃度定量と動態観察を可能とする小分子蛍光プローブの開発 Invited

    Toshiyuki Kowada

    36　(2)　4-9　2021/12

  5. 合成小分子と光の力で細胞内蛋白質の局在を自在に操る！ Invited

    Toshiyuki Kowada

    2021/07

  6. 化学フロンティア研究会―化学を楽しむ人びとが集まる合宿形式の講演会 Invited

    小和田俊行

    月刊「化学」　76　(4)　30-32　2021/04

    Publisher: 化学同人

  7. 一酸化窒素の謎をノックアウト～新しいモジュール型NO検出蛍光プローブの開発～ Invited

    Toshiyuki Kowada

    Chemistry　75　(9)　62-63　2020/09

  8. pH 感受性蛍光プローブを用いた破骨細胞活性の生体イメージング

    Toshiyuki Kowada, Hiroki Maeda, Kazuya Kikuchi

    Chemical Biology　9　(1)　3-6　2016

    Publisher: Japanese Society for Chemical Biology

  9. 二光子励起顕微鏡で捉える硬い骨の中の生命現象

    Toshiyuki KOWADA

    Chemistry & Chemical Industry　67　(8)　698-698　2014/08

    Publisher: The Chemical Society of Japan

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Books and Other Publications 7

  1. Zinc in Biology: Molecular Structures, Cellular Processes and Living Systems

    Rong Liu, Toshiyuki Kowada, Shin Mizukami

    Royal Society of Chemistry　2025/07

    DOI： 10.1039/9781837676583-00001 　

    ISBN: 9781837674565

  2. 実験医学

    小和田俊行, 馬場好花, 水上 進

    羊土社　2025/02

    DOI： 10.18958/6941-00005-0000021-00 　

    ISBN: 9784758125888

  3. Fluorescent Chemosensors

    Toshiyuki Kowada, Shin Mizukami

    Royal Society of Chemistry　2023/04

    DOI： 10.1039/9781839167324-00256 　

    ISBN: 9781839163869

  4. Live Cell Imaging: Methods and Protocols (Methods in Molecular Biology)

    Priya Ranjan Sahoo, Toshiyuki Kowada, Shin Mizukami

    Humana (Springer, New York, US)　2021/07

  5. 実験医学増刊「生きてるものは全部観る！イメージングの選び方・使い方100+」

    Toshiyuki Kowada, Shin Mizukami

    Yodosha　2018/12

  6. Optical Probes in Biology, Edited by Jin Zhang, Sohum Mehta, Carsten Schultz

    Toshiyuki KOWADA, Hiroki MAEDA, Kazuya KIKUCHI

    CRC Press　2015

    ISBN: 9781466510111

  7. 実験医学別冊「in vivo イメージング実験プロトコール 原理と導入のポイントから ２光子顕微鏡の応用まで」

    KOWADA Toshiyuki, KIKUCHI Kazuya

    Yodosha Company Limited　2012/12

    ISBN: 9784758101851

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Presentations 132

  1. Minimal-background chemo-optogenetic protein dimerization system for precise control of cellular signaling International-presentation

    Toshiyuki Kowada, Toshizo Sato, Satoshi Hamaguchi, Konoka Baba, Muhammad Wildan Saifudin, Shin Mizukami

    The 55th Naito Conference “Chemical Biology Empowered by Molecular Transformation Science”　2026/07/02

  2. A multifunctional photochromic dimerizer enabling spatiotemporal control and real-time analysis of organelle dynamics International-presentation

    Konoka Baba, Toshiyuki Kowada, Shin Mizukami

    Gordon Research Conference, Bioorganic Chemistry “Chemistry Unlocking Biology for Therapeutic Discovery”　2026/06/08

  3. Development of a photochromic protein trimerization system enabled by intracellular click chemistry

    Toshiyuki Kowada, Konoka Baba, Shin Mizukami

    2026/06/02

  4. 生体内物質の可視化と局在制御から迫る細胞機能の理解 Invited

    小和田俊行

    先端ケミカルバイオロジー 2026　2026/05/31

  5. Development of small-molecule fluorescent probes for visualizing mitochondrial Zn 2+ dynamics

    EunHye Sung, Toshiyuki Kowada, Shin Mizukami

    The 106th CSJ Annual Meeting　2026/03/19

  6. Quantitative imaging of subcellular labile Zn2+ using a small molecule–fluorescent protein hybrid probe

    Keita Ebine, Toshiyuki Kowada, Shin Mizukami

    The 106th CSJ Annual Meeting　2026/03/18

  7. Optical regulation and functional analysis of organelle membrane contact sites using an in situ click-assembled protein trimerizer

    Konoka Baba, Toshiyuki Kowada, Shin Mizukami

    The 106th CSJ Annual Meeting　2026/03/18

  8. Evaluation of halogen substituent effects of a photochromic eDHFR ligand

    Mai Wako, Ira Novianti, Toshiyuki Kowada, Shin Mizukami

    The 106th CSJ Annual Meeting　2026/03/18

  9. Improved photochromic chemically induced protein dimerization system for precise control of cellular signaling International-presentation

    Toshiyuki Kowada, Toshizo Sato, Satoshi Hamaguchi, Konoka Baba, Muhammad Wildan Saifudin, Shin Mizukami

    Pacifichem 2025　2025/12/18

  10. Development of a clickable photochromic protein dimerizer and optical control of intracellular protein localization International-presentation

    Konoka Baba, Toshiyuki Kowada, Shin Mizukami

    Pacifichem 2025　2025/12/16

  11. Development of Optochemogenetic Technology for Regulating Intracellular Droplet Formation International-presentation

    Muhammad Wildan Saifudin, Toshiyuki Kowada, Hayashi Yamamoto, Shin Mizukami

    Pacifichem 2025　2025/12/16

  12. 光操作プローブの開発と細胞内生体分子機能の光制御 Invited

    Toshiyuki Kowada

    これからの研究とキャリアを考えるワークショップ　2025/11/23

  13. Development of optochemogenetic technology for regulating intracellular droplet formation International-presentation

    Muhammad Wildan Saifudin, Toshiyuki Kowada, Hayashi Yamamoto, Shin Mizukami

    International Symposium on Cellular Structural Biology　2025/09/18

  14. In-cell assembly of a light-controlled protein trimerization system for supracomplex formation International-presentation

    Konoka Baba, Toshiyuki Kowada, Shin Mizukami

    International Symposium on Cellular Structural Biology　2025/09/18

  15. Quantitative imaging of intraorganellar labile Zn2+ using small-molecule–protein hybrid fluorescent probes International-presentation

    Toshiyuki Kowada, Rong Liu, Yuyin Du, EunHye Sung, Shin Mizukami

    International Symposium on Cellular Structural Biology　2025/09/18

  16. In-cell assembly of a light-controlled protein trimerization system for supracomplex formation

    Konoka Baba, Toshiyuki Kowada, Shin Mizukami

    第19回バイオ関連化学シンポジウム　2025/09/02

  17. Fast-Blinking Fluorophores Enabling High-Speed Single-Molecule Super-Resolution Imaging International-presentation

    Bochao Li, Toshiyuki Kowada, Takahiro Fujiwara, Shin Mizukami

    19th Conference on Methods and Applications in Fluorescence (MAF2025)　2025/08/25

  18. 多機能性フォトクロミック蛋白質二量化剤の開発

    Konoka Baba, Toshiyuki Kowada, Shin Mizukami

    2025 年生体機能関連化学・生物無機化学・生命金属科学合同サマースクール　2025/07/08

  19. 多機能化可能なフォトクロミック蛋白質二量化剤の開発

    馬場 好花, 小和田 俊行, 水上 進

    日本化学会第105春季年会　2025/03/28

  20. Live-cell super-resolution imaging of mitochondrial inner membranes using a fast-blinking fluorophore

    Bochao Li, Toshiyuki Kowada, Takahiro Fujiwara, Shin Mizukami

    日本化学会第105春季年会　2025/03/26

  21. Degradation of lipid droplets by in-cell self-assembly of autophagy-tethering chimeras

    Yi Ding, Ira Novianti, Toshiyuki Kowada, Shin Mizukami

    日本化学会第105春季年会　2025/03/26

  22. Development of Optochemogenetic Technology for Regulating Intracellular Droplet Formation

    Muhammad Wildan Saifudin, Toshiyuki Kowada, Hayashi Yamamoto, Shin Mizukami

    日本化学会第105春季年会　2025/03/26

  23. Development of a mitochondria-targeting fluorescent probe for visualizing Zn2+ flux

    EunHye Sung, Toshiyuki Kowada, Shin Mizukami

    日本化学会第105春季年会　2025/03/26

  24. Development of Optochemogenetic Technology for Regulating Intracellular Droplet Formation

    Muhammad W. Saifudin, Toshiyuki Kowada, Shin Mizukami

    2024/12/12

  25. クリック反応により機能化可能なフォトクロミック蛋白質二量化剤の開発と細胞内蛋白質局在の光制御への応用

    馬場 好花, 小和田 俊行, 水上 進

    第24回東北大学多元物質科学研究所研究発表会　2024/12/12

  26. Photoactivatable Macrocyclic Tetrazine Enables Spatial Tagging of Mitochondria for Controlled Delivery of Small Molecules

    Ira Novianti, Toshiyuki Kowada, Shin Mizukami

    2024/12/12

  27. Fluorescence imaging and quantification of intraorganelle labile zinc ions Invited

    Toshiyuki KOWADA

    2024/11/27

  28. 化学プローブを用いた細胞内蛋白質局在の操作技術の開発 Invited

    小和田俊行

    第13回化学フロンティア研究会　2024/10/27

  29. 機能性小分子による細胞内イオン動態の可視化と光制御 Invited

    Toshiyuki Kowada

    次世代薬理学セミナー 2024 in 岩手　2024/09/21

  30. Precise control of subcellular protein localization by improved photoreversible protein labeling system

    Toshiyuki Kowada, Toshizo Sato, Satoshi Hamaguchi, Muhammad Wildan Saifudin, Shin Mizukami

    第18回バイオ関連化学シンポジウム　2024/09/12

  31. Development of fast-blinking fluorophores for super-resolution live imaging of the inner mitochondrial membrane International-presentation

    Bochao Li, Toshiyuki Kowada, Takahiro Fujiwara, Shin Mizukami

    Tohoku University's Chemistry Summer School 2024　2024/07/19

  32. 低分子－蛍光蛋白質ハイブリッド型プローブによる細胞内亜鉛イオンの定量イメージング

    小和田 俊行, 劉 熔, 水上 進

    第3回生命金属科学シンポジウム　2024/06/23

  33. Precise control of subcellular protein localization by improved photoreversible protein labeling system

    2024/05/30

  34. Precise control of subcellular protein localization by improved photoreversible protein labeling system International-presentation

    Toshiyuki Kowada, Toshizo Sato, Satoshi Hamaguchi, Shin Mizukami

    The 3rd International Symposium on Biofunctional Chemistry　2024/04/24

  35. Development of fast-blinking fluorophores for real-time single-molecule super-resolution imaging International-presentation

    Bochao Li, Toshiyuki Kowada, Takahiro Fujiwara, Shin Mizukami

    The 3rd International Symposium on Biofunctional Chemistry　2024/04/24

  36. Development of fluorescent probes to detect sulfatase 2 activity for tumor imaging International-presentation

    Yi Ding, Toshiyuki Kowada, Norihiko Sasaki, Toshitaka Matsui, Shin Mizukami

    The 3rd International Symposium on Biofunctional Chemistry　2024/04/24

  37. Development of fast-blinking fluorophores for real-time single-molecule super-resolution imaging

    Bochao Li, Toshiyuki Kowada, Takahiro Fujiwara, Shin Mizukami

    2023/12/07

  38. フォトクロミック化合物を用いたタグ融合蛋白質の光分解技術の開発

    松尾 章弘, 小和田 俊行, 水上 進

    第13回CSJ化学フェスタ2023　2023/10/18

  39. フォトクロミック蛋白質二量化剤を用いた細胞内蛋白質局在の光制御法の開発

    佐藤 歳三, 小和田 俊行, 水上 進

    第13回CSJ化学フェスタ2023　2023/10/18

  40. 細胞内金属イオンの可視化・定量・操作 Invited

    小和田 俊行

    メタルバイオサイエンス研究会2023　2023/10/06

  41. Development of photo-degradation technology of tag-fused proteins

    Toshiyuki Kowada, Akihiro Matsuo, Himadri S. Sarkar, Toshitaka Matsui, Shin Mizukami

    2023/09/08

  42. Development of fast blinking fluorophores for real-time single-molecule super-resolution imaging International-presentation

    Bochao Li, Toshiyuki Kowada, Takahiro Fujiwara, Shin Mizukami

    The 3rd Meeting of Cross-Scale Biology　2023/09/07

  43. Development of fluorescent probes to detect sulfatase 2 activity for tumor imaging International-presentation

    Yi Ding, Toshiyuki Kowada, Norihiko Sasaki, Toshitaka Matsui, Shin Mizukami

    The 3rd Meeting of Cross-Scale Biology　2023/09/07

  44. Optical regulation of protein-protein interactions in living cells using a photochromic dimerizer International-presentation Invited

    Toshiyuki Kowada

    6th Asian Chemical Biology Conference 2023 (ACBC2023)　2023/08/22

  45. Development of fluorescent probes to detect sulfatase 2 activity for tumor imaging International-presentation

    Yi Ding, Toshiyuki Kowada, Norihiko Sasaki, Toshitaka Matsui, Shin Mizukami

    Tohoku University's Chemistry Summer School 2023　2023/08/09

  46. Development of fast-blinking fluorophores for real-time single-molecule super-resolution imaging International-presentation

    Bochao Li, Toshiyuki Kowada, Takahiro Fujiwara, Shin Mizukami

    Tohoku University's Chemistry Summer School 2023　2023/08/09

  47. Development of photo-responsive protein degraders for tag-fused proteins International-presentation

    Akihiro Matsuo, Toshiyuki Kowada, Himadri S. Sarkar, Toshitaka Matsui, Shin Mizukami

    Tohoku University's Chemistry Summer School 2023　2023/08/09

  48. Quantitative imaging of subcellular Zn2+ using localizable small-molecule fluorescent probes International-presentation

    Rong Liu, Toshiyuki Kowada, Shin Mizukami

    Tohoku University's Chemistry Summer School 2023　2023/08/09

  49. Development of an optochemical tool controlling the formation and dissociation of mitochondria–endoplasmic reticulum contact sites International-presentation

    Quan Minh Duong, Toshiyuki Kowada, Shin Mizukami

    Tohoku University's Chemistry Summer School 2023　2023/08/08

  50. Development of fluorescent probes to detect sulfatase 2 activity for tumor imaging

    Yi Ding, Toshiyuki Kowada, Norihiko Sasaki, Toshitaka Matsui, Shin Mizukami

    2023/06/09

  51. Development of photochromic protein dimerizers having an azopyrazole moiety and optical regulation of intracellular protein localization

    2023/06/08

  52. Development of an optochemical tool controlling the formation of mitochondria–endoplasmic reticulum contact sites

    Quan Minh Duong, Toshiyuki Kowada, Shin Mizukami

    2023/05/30

  53. 生命金属科学研究のための化学プローブ開発 Invited

    小和田 俊行

    第2回生命金属科学シンポジウム　2023/05/20

  54. フォトクロミック蛋白質二量化剤を用いた生細胞内蛋白質局在の光制御

    佐藤 歳三, 小和田 俊行, 濱口 聡志, 松井 敏高, 水上 進

    日本化学会第103春季年会　2023/03/23

  55. Development of fast blinking fluorophores for real-time single-molecule super-resolution imaging

    Bochao Li, Toshiyuki Kowada, Takahiro Fujiwara, Shin Mizukami

    2023/03/23

  56. 蛋白質ラベル化の光制御法の開発と生命システムの理解 Invited

    小和田 俊行

    日本化学会第103春季年会　2023/03/23

  57. タグ融合蛋白質の細胞内分解を光制御する機能性化合物の開発

    松尾 章弘, 小和田 俊行, Himadri S. Sarkar, 松井 敏高, 水上 進

    日本化学会第103春季年会　2023/03/22

  58. Activatable Click Reaction using Macrocyclic Tetrazines

    Ira Novianti, Toshiyuki Kowada, Shin Mizukami

    2022/12/08

  59. Quantitative Mapping of Subcellular Labile Zn2+ Concentrations Using Localizable Small-Molecule Fluorescent Probes International-presentation

    Rong Liu, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami

    10th Asian Biological Inorganic Chemistry Conference (AsBIC10)　2022/11/30

  60. Time-Lapse Ratiometric Imaging of Mitochondrial pH during Mitophagy Using a Localizable Fluorescent Probe International-presentation

    Toshiyuki Kowada, Risa Ito, Makoto Iwashita, Toshitaka Matsui, Shin Mizukami (Speaker)

    10th Asian Biological Inorganic Chemistry Conference (AsBIC10)　2022/11/29

  61. Quantitative imaging of organellar labile Zn2+ using localizable small-molecule fluorescent probes International-presentation Invited

    Toshiyuki Kowada

    10th Asian Biological Inorganic Chemistry Conference (AsBIC10)　2022/11/30

  62. Molecular basis of redox- and zinc-dependent protein quality control at the ER-Golgi interface International-presentation Invited

    Kenji Inaba (Invited speaker), Satoshi Watanabe, Yuta Amagai, Bui Ba Han, Toshiyuki Kowada, Shin Mizukami, Roberto Sitia

    Redox Week in Sendai (4th International Conference on Persulfide and Sulfur Metabolism in Biology and Medicine)　2022/10/31

  63. Development of small-molecule fluorescent probes for the quantitative analysis of labile Zn2+ in cells Invited

    Toshiyuki Kowada

    The 60th Annual Meeting of the Biophysical Society of Japan　2022/09/28

  64. Development of photochromic ligands for quantitative and photo-reversible protein labeling

    Satoshi Hamaguchi, Toshiyuki Kowada, Takato Mashita, Himadri S. Sarkar, Toshitaka Matsui, Shin Mizukami

    The 16th Symposium on Biorelevant Chemistry, CSJ　2022/09/12

  65. 光可逆的蛋白質ラベル化法に基づく細胞内蛋白質局在の光制御

    小和田俊行, 間下貴斗, 山本 林, 松井敏高, 水上進

    クロススケール新生物学 第２回領域会議　2022/07/28

  66. Optical control of intracellular protein localization based on a photo-reversible protein labeling system

    2022/05/27

  67. Optical control of intracellular protein localization based on a photo-reversible protein labeling system

    2022/05/27

  68. Optical regulation of protein translocation using a photo-reversible protein labeling system

    Takato Mashita, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami

    The 102nd CSJ Annual Meeting　2022/03/24

  69. 局在型レシオ蛍光プローブを用いた細胞内局所pHの定量イメージング

    岩下 誠, 小和田俊行, 伊藤理紗, 松井敏高, 水上 進

    日本化学会第102春季年会　2022/03/24

  70. Controlling IEDDA Reaction with Macrocyclic Tetrazine

    Ira Novianti, Toshiyuki Kowada, Shin Mizukami

    The 102nd CSJ Annual Meeting　2022/03/23

  71. Development of photo-reversible protein labeling system

    間下貴斗, 小和田俊行, 松井敏高, 水上 進

    2021/12/13

  72. Development of fluorescent probes for visualizing Zn2+/H+ dynamics in living cells

    小和田俊行, 松井敏高, 水上 進

    学術変⾰領域（A）クロススケール新⽣物学 第一回領域会議　2021/12/13

  73. Development of novel fluorescent probes for detection of extracellular sulfatases

    Yi Ding, Kashfia Ahamed, Toshiyuki Kowada, Norihiko Sasaki, Toshitaka Matsui, Shin Mizukami

    2021/12/09

  74. 細胞内pH変化のリアルタイムイメージング用蛍光プローブの開発

    岩下 誠, 小和田俊行, 伊藤理紗, 松井敏高, 水上 進

    第21回東北大学多元物質科学研究所研究発表会　2021/12/09

  75. Development of a chemical biology tool enabling reversible optical control of protein labeling International-presentation

    Himadri Sekhar Sarkar, Takato Mashita, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami

    The 10th Annual Conference Of The International Chemical Biology Society (ICBS2021)　2021/11/11

  76. Development of small-molecule fluorescent probes for quantification of labile Zn2+ concentration and monitoring of Zn2+ dynamics in organelles

    Toshiyuki Kowada, Rong Liu, Yuyin Du, Toshitaka Matsui, Shin MIzukami

    2021/09/08

  77. Regulated Zn2+ concentrations at the early secretory pathway control function of an ER-Golgi chaperone, ERp44

    2021/06/16

  78. Optical control of intracellular protein dimerization using covalent protein labeling technology

    2021/05/27

  79. Optical control of intracellular protein dimerization using covalent protein labeling technology

    2021/05/26

  80. Quantitative imaging of labile Zn2+ using organelle-localizable fluorescent probes

    Rong Liu, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami

    2021/05/26

  81. Visualization and quantification of labile Zn2+ in the acidic subcellular compartments using a small-molecule fluorescent probe

    Yuyin Du, Rong Liu, Toshiyuki Kowada, Toshitaka Matsui, Hiroshi Kadokura, Kenji Inaba, Shin Mizukami

    2021/05/15

  82. Quantitative Mapping of Cellular Labile Zn2+ via Development of Hybrid Fluorescent Probes

    Rong Liu, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami

    2021/03/19

  83. Development of a novel fluorescent probe for detection of extracellular sulfatase

    Kashfia Ahamed, Toshiyuki Kowada, Norihiko Sasaki, Shin Mizukami

    2021/03/19

  84. 哺乳動物細胞分泌経路における亜鉛調節とタンパク質品質管理

    天貝佑太, 山田桃, 渡邊朝美, 小和田俊行, 楢本悟史, 渡部聡, 経塚淳子, Roberto Sitia, 水上進, 稲葉謙次

    第62回日本植物生理学会年会　2021/03/15

  85. Escherichia coli dihydrofolate reductase-selective photoswitchable inhibitors enabling reversible optical control of protein labeling

    Himadri Sekhar Sarkar, Takato Mashita, Toshiyuki Kowada, Ming Ming Yem, Satoshi, Watanabe, Kenji Inaba, Toshitaka Matsui, Shin Mizukami

    2020/12/03

  86. Subcellular Labeling Performances of Tetrazine–trans-Cyclooctene Cycloadditions and Their Products

    Ira Novianti, Toshiyuki Kowada, Shin Mizukami

    2020/12/03

  87. Development of organelle-localizable fluorescent probes and quantitative imaging of intracellular labile Zn2+

    Toshiyuki Kowada, Tomomi Watanabe, Yuta Amagai, Rong Liu, Momo Yamada, Hiroto, Takahashi, Toshitaka Matsui, Kenji Inaba, Shin Mizukami

    The 29th annual meeting of the bioimaging society　2020/11/21

  88. 光可逆的な蛋白質ラベル化を可能とするリガンドの開発

    間下 貴斗, 小和田 俊行, SARKAR S. Himadri, 高橋 泰人, 松井 敏高, 水上 進

    第31回万有仙台シンポジウム　2020/10/17

  89. Quantitative Mapping of Organellar Labile Zn2+ via the Development of Hybrid Fluorescent Probes

    LIU, Rong, KOWADA, Toshiyuki, MATSUI, Toshitaka, MIZUKAMI, Shin

    2020/09/08

  90. Development of photochromic ligands that allow optical regulation of a binding affinity toward the target protein

    MASHITA, Takato, KOWADA, Toshiyuki, SARKAR, Himadri S, TAKAHASHI, Hiroto, MATSUI Toshitaka, MIZUKAMI Shin

    2020/09/08

  91. Quantitative Mapping of Cellular Labile Zn2+ via Development of Hybrid Fluorescent Probes

    LIU, Rong, KOWADA, Toshiyuki, WATANABE, Tomomi, MATSUI, Toshitaka, MIZUKAMI, Shin

    The 100th CSJ Annual Meeting　2020/03/22

  92. Development of photoswitchable ligands for protein labeling system

    Takato Mashita, Toshiyuki Kowada, Himadri Sekhar Sarkar, Hiroto Takahashi, Toshitaka Matsui, Shin Mizukami

    The 100th CSJ Annual Meeting　2020/03/22

  93. Quantitative imaging analysis of labile Zn2+ in intracellular organelles via the development of hybrid fluorescent probe

    Rong LIU, Toshiyuki KOWADA, Tomomi WATANABE, Tshitaka MATSUI, Shin MIZUKAMI

    2020/02/14

  94. Development of photochromic ligands that enable photoreversible protein labeling International-presentation

    Takato Mashita, Toshiyuki Kowada, Hiroto Takahashi, Toshitaka Matsui, Shin Mizukami

    Chemical Biology and Physiology Conference 2019　2019/12/14

  95. Development of fluorescent probes for visualization and quantitative analysis of free zinc ion in intracellular organelles International-presentation

    Toshiyuki Kowada, Tomomi Watanabe, Rong Liu, Toshitaka Matsui, Shin Mizukami

    Chemical Biology and Physiology Conference 2019　2019/12/14

  96. Quantitative Mapping of Cellular Labile Zn2+ via Development of Hybrid Fluorescent Probes

    Rong Liu, Toshiyuki Kowada, Tomomi Watanabe, Toshitaka Matsui, Shin Mizukami

    第19回東北大学多元物質科学研究所研究発表会　2019/12/12

  97. Development of a small-molecule probe for quantitative analysis of organellar labile Zn2+ Invited

    Toshiyuki Kowada

    2019/12/10

  98. ZnT7 regulates ERp44 through the control of Zn2+ concentrations at the ERGIC/cis-Golgi

    Yuta Amagai, Momo Yamada, Tomomi Watanabe, Toshiyuki Kowada, Satoshi Naramoto, Satoshi Watanabe, Junko Kyozuka, Roberto Sitia, Shin Mizukami, Kenji Inaba

    The 42nd Annual Meeting of the Molecular Biology Society of Japan　2019/12/04

  99. Design and synthesis of functional small-molecule probes for fluorescence imaging International-presentation Invited

    Toshiyuki Kowada

    The 6th International Symposium on Bioimaging　2019/09/23

  100. Development of a bioorthogonal ligand that allows photoreversible protein labeling

    MASHITA, Takato, KOWADA, Toshiyuki, TAKAHASHI, Hiroto, MATSUI, Toshitaka, MIZUKAMI, Shin

    第13回バイオ関連化学シンポジウム　2019/09/05

  101. Optical control of biomolecular localization based on photoactivatable protein labeling system

    Michiyuki Suzuki, Toshiyuki Kowada, Keisuke Arai, Akimasa Yoshimura, Toshitaka Matsui, Kazuya Kikuchi, Shin Mizukami

    The 13th Bio-Related Chemistry Symposium　2019/09/05

  102. Development of fluorescent probes for analysis of zinc ion concentration in intracellular organelles

    Toshiyuki Kowada, Rong Liu, Tomomi Watanabe, Toshitaka Matsui, Shin Mizukami

    The 13th Bio-Related Chemistry Symposium　2019/09/04

  103. Development of Fluorescent Probes for Visualization and Quantification of Zn2+ in Organelles International-presentation

    Rong Liu, Toshiyuki Kowada, Tomomi Watanabe, Toshitaka Matsui, Shin Mizukami

    Tohoku University's Chemistry Summer School 2019　2019/08/27

  104. Development of photoswitchable compounds that selectively bind to Escherichia coli dihydrofolate reductase International-presentation

    Takato Mashita, Toshiyuki Kowada, Hiroto Takahashi, Toshitaka Matsui, Shin Mizukami

    Tohoku University's Chemistry Summer School 2018　2019/08/27

  105. Synthesis of functional small-molecule fluorescent probes for clarifying cell function Invited

    Toshiyuki Kowada

    The 8th Chemical Frontier Workshop　2019/08/24

  106. 光可逆的な蛋白質ラベル化技術を指向した機能性小分子の開発

    Takato Mashita, Toshiyuki Kowada, Hiroto Takahashi, Toshitaka Matsui, Shin Mizukami

    生体機能関連化学部会若手の会 第31回サマースクール　2019/07/16

  107. Molecular mechanisms for the zinc-regulated protein quality control in the early secretory pathway

    Yuta Amagai, Momo Yamada, Tomomi Watanabe, Toshiyuki Kowada, Satoshi Watanabe, Shin Mizukami, Kenji Inaba

    第19回日本蛋白質科学会年会 第71回日本細胞生物学会大会 合同年次大会　2019/06/24

  108. Quantitative analysis of free zinc ion concentration in living cells using novel targetable fluorescent probe International-presentation

    Tomomi Watanabe, Toshiyuki Kowada, Rong Liu, Toshitaka Matsui, Shin Mizukami

    15th International Symposium on Applied Bioinorganic Chemistry　2019/06/04

  109. Development of methotrexate derivatives for reversible photo-control of binding to protein

    Takato Mashita, Toshiyuki Kowada, Hiroto Takahashi, Toshitaka Matsui, Shin Mizukami

    日本化学会第99春季年会　2019/03/18

  110. Photo-regulation of protein dimerization using protein labeling system

    Toshiyuki Kowada, Keisuke Arai, Akimasa Yoshimura, Toshitaka Matsui, Kazuya Kikuchi, Shin Mizukami

    The 99th CSJ Annual Meeting　2019/03/18

  111. Visualization of zinc ion in intracellular organelles

    Tomomi Watanabe, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami

    The 99th CSJ Annual Meeting　2019/03/16

  112. Development of fluorescent probe for visualization and quantitative analysis of zinc ion in intracellular organelles International-presentation

    Toshiyuki Kowada, Tomomi Watanabe, Toshitaka Matsui, Shin Mizukami

    The 2nd Symposium for World Leading Research Centers　2019/02/17

  113. Development of light-switchable protein-protein interaction system for protein degradation International-presentation

    Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami

    The 1st International Symposium on Chemical Communication (ISCC2019)　2019/01/10

  114. Visualization of zinc ion in intracellular organelles

    Tomomi Watanabe, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami

    第18回多元研研究発表会　2018/12/13

  115. 細胞内オルガネラにおける亜鉛イオン検出を可能とする小分子蛍光プローブの開発

    小和田 俊行

    第6回アライアンス若手研究交流会　2018/11/01

  116. Development of a protein ligand capable of photoreversible binding and dissociation

    Takato Mashita, Toshiyuki Kowada, Hiroto Takahashi, Toshitaka Matsui, Shin Mizukami

    第12回バイオ関連化学シンポジウム　2018/09/10

  117. Fluorescent probes for quantitative measurement of local pH in cells

    Risa Ito, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami

    第12回バイオ関連化学シンポジウム　2018/09/09

  118. Development of Photoactivatable protein labeling system International-presentation

    Keisuke Arai, Akimasa Yoshimura, Toshitaka Matsui, Kazuya Kikuchi, Shin Mizukami

    Tohoku University's Chemistry Summer School 2018　2018/08/27

  119. Development of fluorescent probe for visualizing intracellular Mg2+ dynamics International-presentation

    Keisuke Wakabayashi, Toshiyuki Kowada, Shin Mizukami

    Tohoku University's Chemistry Summer School 2018　2018/08/27

  120. Development of fluorescent probes for real-time and quantitative measurement of local pH changes in cell International-presentation

    Risa Ito, Toshiyuki Kowada, Shin Mizukami

    Tohoku University's Chemistry Summer School 2018　2018/08/27

  121. Development of fluorescent probes for visualization of zinc ion in intracellular organelles International-presentation

    Tomomi Watanabe, Toshiyuki Kowada, Toshitaka Matsui, Shin Mizukami

    Tohoku University's Chemistry Summer School 2018　2018/08/27

  122. Photo-switchable Dihydrofolate Reductase (DHFR) Inhibitor International-presentation

    Takato Mashita, Toshiyuki Kowada, Hiroto Takahashi, Toshitaka Matsui, Shin Mizukami

    Tohoku University's Chemistry Summer School 2018　2018/08/27

  123. Development of self-assembling peptide for controlling aggregation state by light International-presentation

    Yuri Hashimoto, Toshiyuki Kowada, Shin Mizukami

    Tohoku University's Chemistry Summer School 2018　2018/08/27

  124. Live-cell fluorescence imaging of zinc ions using localizable small-molecule probe International-presentation

    Toshiyuki Kowada, Tomomi Watanabe, Toshitaka Matsui, Shin Mizukami

    5th CWRU-Tohoku Joint Workshop　2018/08/02

  125. Development of functional small-molecule fluorescent probes for analysis of biological functions Invited

    小和田俊行

    平成30年度東日本分析化学若手交流会　2018/07/06

  126. Visualization of zinc ion dynamics in intracellular organelles

    渡邊 朝美, 小和田 俊行, 松井 敏高, 水上 進

    第28回金属の関与する生体関連反応シンポジウム（SRM2018）　2018/06/29

  127. Creation of light-switchable protein degradation system

    小和田 俊行

    新学術領域「化学コミュニケーションのフロンティア」第3回公開シンポジウム　2018/06/27

  128. Development of Reversibly Photoresponsive Drug

    間下 貴斗, 小和田 俊行, 高橋 泰人, 松井 敏高, 水上 進

    第29回 万有仙台シンポジウム　2018/06/09

  129. Synthesis and functional assessment of a photoresponsive compound for bioanalytical applications

    Takato Mashita, Toshiyuki Kowada, Hiroto Takahashi, Toshitaka Matsui, Shin Mizukami

    日本化学会第98春季年会　2018/03/22

  130. Synthesis and functional assessment of a photoresponsive compound for bioanalytical applications

    Takato Mashita, Toshiyuki Kowada, Hiroto Takahashi, Toshitaka Matsui, Shin Mizukami

    日本化学会第98春季年会　2018/03/20

  131. 化学プローブを用いた生体機能の可視化 Invited

    小和田 俊行

    第7回生命科学研究科交流ミーティング　2017/06/02

  132. Real-time intravital imaging of pH-variation associated with osteoclast activity using small- molecule fluorescent probe International-presentation Invited

    Toshiyuki Kowada

    The 21st International Symposium on Institute for Integrated Cell-Material Sciences　2016/06/03

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Research Projects 18

  1. オルガネラ間コンタクトサイト形成の可逆的光操作技術の開発 Competitive

    小和田 俊行

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業

    Category: 基盤研究(B)

    Institution: 東北大学

    2026/04 - 2029/03

  2. 人工オルガネラ「メタロソーム」の創製に基づく生命現象の 制御と理解 Competitive

    Offer Organization: JST（国立研究開発法人 科学技術振興機構）

    System: 2024年度 創発的研究支援事業

    2025/10 - 2029/03

  3. Next-Generation Fluorogenic Probes for Wash-Free Cell Surface Imaging Competitive

    Shahi Imam REJA, 小和田俊行

    Offer Organization: 東北大学附置研究所・センター連携体

    System: 2026年度若手研究者アンサンブルグラント

    Institution: 東北大学

    2026/06 - 2027/03

  4. オルガネラ間膜接触領域形成の光制御法の開発とイオン流動解析

    小和田 俊行

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(B)

    Category: 基盤研究(B)

    Institution: 東北大学

    2023/04 - 2026/03

  5. オルガネラ間膜接触領域形成の生理的意義理解に向けた光操作化学ツールの開発

    Offer Organization: 公益財団法人 コニカミノルタ科学技術振興財団

    System: 令和4年度コニカミノルタ画像科学奨励賞

    2023/04 - 2024/03

  6. 細胞小器官同士の膜接触領域の形成と解離を光制御する化学ツールの開発

    Offer Organization: 公益財団法人 稲盛財団

    System: 2022年度稲盛研究助成

    Institution: 東北大学

    2022/04 - 2023/03

  7. 環状リガンドの配座制御を基盤としたタンパク質局在の光制御法の開発 Competitive

    小和田 俊行

    Offer Organization: 日本学術振興会

    System: 科学研究費助成事業 基盤研究(C)

    Category: 基盤研究(C)

    Institution: 東北大学

    2020/04 - 2023/03

  8. フッ素原子の特異な性質を利用した長波長蛍光色素群の網羅的合成と生命科学研究への応用

    Offer Organization: 公益社団法人 有機合成化学協会

    System: 令和元年度（第32回）有機合成化学協会研究企画賞

    Institution: 東北大学

    2020/04 - 2022/03

  9. 細胞内亜鉛イオン濃度マップ作成に向けた蛍光プローブの開発

    Offer Organization: 東北大学多元物質科学研究所

    System: 2019年度多元研プロジェクト

    Institution: 東北大学

    2019/04 - 2020/03

  10. 化学シグナル解析に資するタンパク質分解光制御法の創出 Competitive

    小和田 俊行

    Offer Organization: 文部科学省

    System: 科学研究費補助金, 新学術領域研究

    2018/04 - 2020/03

  11. Development of chemical tools for photocontrol of protein-protein interaction Competitive

    KOWADA Toshiyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grant-in-Aid for Scientific Research

    2017/04 - 2020/03

  12. 機能性小分子を用いた生体分子機能の光操作法の確立

    Offer Organization: 東北大学多元物質科学研究所

    System: 平成30年度多元研プロジェクト

    Institution: 東北大学

    2018/06 - 2019/03

  13. シグナル伝達経路の詳細解析を可能とする光操作小分子プローブの開発

    Offer Organization: 公益財団法人 中谷医工計測技術振興財団

    System: 平成29年度奨励研究助成

    Institution: 東北大学

    2018/04 - 2019/03

  14. 生体機能の可逆的光制御を可能とする化学ツールの開発

    Offer Organization: 公益財団法人 興和生命科学振興財団

    System: 平成29年度研究助成

    Institution: 東北大学

    2017/12 - 2018/11

  15. 生体機能の光制御を可能にする化学ツールの開発

    Offer Organization: 公益財団法人 武田科学振興財団

    System: 2017年度ライフサイエンス研究奨励

    Institution: 東北大学

    2017/04 -

  16. Development of fluorescent probes for visualizing low-pH environment and their application in intravital imaging Competitive

    KOWADA Toshiyuki

    Offer Organization: Japan Society for the Promotion of Science

    System: Grant-in-Aid for Scientific Research

    2012/04 - 2014/03

  17. 分子プローブの開発とセラノスティクス

    Offer Organization: The Naito Foundation

    System: Grant for Studying Overseas

    2014 -

  18. Visualization of osteoclast function using pH-activatable fluorescent probes

    Offer Organization: Immunology Frontier Research Center (IFReC)

    Institution: Osaka University

    2010/10 - 2013/09

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Teaching Experience 8

  1. Biochemistry IIA　(AMC course, Tohoku University) Undergraduate (specialized)

  2. 先端分子化学生物学特論Ⅲ

  3. 生命科学B（全学教育科⽬）　東北大学 Undergraduate (liberal arts)

  4. Exercises in Organic Chemistry A

  5. Exercises in Organic Chemistry B

  6. Laboratory Experiments in Chemistry

  7. 学問論演習　全学教育科目, 東北大学

  8. 基礎ゼミ　全学教育科目, 東北大学

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